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<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4E7</accession>
    <name>D5G4E7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00004075001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4E7"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004075001"/>
    <dbReference type="HOGENOM" id="CLU_2656248_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4E7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="7"/>
            <end position="25"/>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="76" mass="8233" checksum="70F155F8917FD704" modified="2010-06-15" version="1">MMITRVVRGGVTCLSAVSIVSIYRIKFVPPHPGGGLFCFAFGFILSALPRCYRTVRVLGFTSSRALCSAGDSTRFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4N5</accession>
    <name>D5G4N5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00000026001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429989">
        <property type="protein sequence ID" value="CAZ79478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835321.1">
        <property type="nucleotide sequence ID" value="XM_002835275.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4N5"/>
    <dbReference type="STRING" id="656061.D5G4N5"/>
    <dbReference type="GeneID" id="9188414"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000026001"/>
    <dbReference type="eggNOG" id="ENOG502SHBR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_525990_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4N5"/>
    <dbReference type="OMA" id="TITWNEV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="206"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="206"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="228"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="518" mass="56715" checksum="FC6F697DB0918E05" modified="2010-06-15" version="1">MSPGDINPLDEWIRRQSFKIAYSDMTKGFIGKKALKEAICSSIPAEFGARGYTPEKVRIRLKAMMAERLTSELVRECDARRVASGAAAGNLGTGGIASNGRAASAGAAVGQEMPSREVRGGQSRVLDKGKQRAMDNEERTKAPLHMSSYRTAMPVGVPPSAGGLAPKKKPSECRSPELEVSDEILEGYKRAAAAGVRILKTPTNDSAVPSLGESTRTTALPRSPSILSTSANASTTRTAPSTVTADSVIPSLPFKLQYRIFTALQAILESCCYDFARRYYPQYLRRKSWDCPEAGELTAWIRGLSKEFSSNPVFDMGEDEFDLILKKGGEIRHAAVHRVSVSGLEIQSLIEDARAMAEILDDEEVEGRLREIDEIKVVVDNNIQTLLDRRAAKEEKLRLELEKLERWKRDIELREVMATKGAEEEEESIRRDFQDGIGRELEWLGLEGKGLGVRDRREAIEVAGSASTSAPRGEGAEALATITENAEEDFHEPEGWPAAEDAPLERLLAKGGISPLSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>D5G537</accession>
    <name>D5G537_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Eukaryotic translation initiation factor 3 subunit G</fullName>
            <shortName evidence="5">eIF3g</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Eukaryotic translation initiation factor 3 RNA-binding subunit</fullName>
            <shortName evidence="5">eIF-3 RNA-binding subunit</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Translation initiation factor eIF3 p33 subunit homolog</fullName>
            <shortName evidence="5">eIF3 p33 homolog</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">TIF35</name>
        <name evidence="9" type="ORF">GSTUM_00000237001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation. This subunit can bind 18S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the eIF-3 subunit G family.</text>
    </comment>
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        <property type="protein sequence ID" value="CAZ79622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002835419.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G537"/>
    <dbReference type="FunCoup" id="D5G537">
        <property type="interactions" value="1058"/>
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    <dbReference type="STRING" id="656061.D5G537"/>
    <dbReference type="GeneID" id="9186320"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000237001"/>
    <dbReference type="eggNOG" id="KOG0122">
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    <dbReference type="HOGENOM" id="CLU_034595_0_0_1"/>
    <dbReference type="InParanoid" id="D5G537"/>
    <dbReference type="OMA" id="ICQGDHF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071540">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3e"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071541">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3m"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043614">
        <property type="term" value="C:multi-eIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002188">
        <property type="term" value="P:translation reinitiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006415">
        <property type="term" value="P:translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12933">
        <property type="entry name" value="eIF3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12408">
        <property type="entry name" value="RRM_eIF3G_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000328">
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        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.30.70.330">
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    </dbReference>
    <dbReference type="HAMAP" id="MF_03006">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017334">
        <property type="entry name" value="eIF3_g"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024675">
        <property type="entry name" value="eIF3g_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034240">
        <property type="entry name" value="eIF3G_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10352">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12353">
        <property type="entry name" value="eIF3g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037949">
        <property type="entry name" value="Transl_init_eIF-3_RNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4 5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="210"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="155"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="155"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="171"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ79622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="289" mass="31809" checksum="6395A856FECC009D" modified="2010-06-15" version="1">MPDIASSRANWADDEEFDDPSVLPPQQVINNPDGTKTVVTYRFNDNGKKVKTTRKIRTTIVKEHVNPRVAERKEWAKFGAEKGHSRGPDLSTTSVGENIIFRPSTNWKSTSKEDNTEENKLKDQLKDKKVACRICRGDHFTARCPFKDTLPPLDDAAAPAAEESPAADGPGAIGGSGGGSYVAPHLRGKKGAGESMSGTGGSYKDRDDLATLRVTNVSEFAEESDLREMFERFGRVTRVFLAKDRDTGRAKGFAFISYVDRSDAAKACERMDGFGYGHLILRVEFAKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5G546</accession>
    <name>D5G546_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 19">Autophagy-related protein 9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">GSTUM_00000250001</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Phospholipid scramblase involved in autophagy. Cycles between the preautophagosomal structure/phagophore assembly site (PAS) and the cytoplasmic vesicle pool and supplies membrane for the growing autophagosome. Lipid scramblase activity plays a key role in preautophagosomal structure/phagophore assembly by distributing the phospholipids that arrive through ATG2 from the cytoplasmic to the luminal leaflet of the bilayer, thereby driving autophagosomal membrane expansion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate)(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate)(out)</text>
            <dbReference type="Rhea" id="RHEA:67920"/>
            <dbReference type="ChEBI" id="CHEBI:58088"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out)</text>
            <dbReference type="Rhea" id="RHEA:38663"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine(in) = a 1,2-diacyl-sn-glycero-3-phosphocholine(out)</text>
            <dbReference type="Rhea" id="RHEA:38571"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out)</text>
            <dbReference type="Rhea" id="RHEA:38895"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Golgi apparatus membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3 19">Preautophagosomal structure membrane</location>
            <topology evidence="3 19">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 19">Belongs to the ATG9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="FN429996">
        <property type="protein sequence ID" value="CAZ79631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835474.1">
        <property type="nucleotide sequence ID" value="XM_002835428.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G546"/>
    <dbReference type="FunCoup" id="D5G546">
        <property type="interactions" value="210"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G546"/>
    <dbReference type="GeneID" id="9186329"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000250001"/>
    <dbReference type="eggNOG" id="KOG2173">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006200_1_1_1"/>
    <dbReference type="InParanoid" id="D5G546"/>
    <dbReference type="OMA" id="YKWANVE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061908">
        <property type="term" value="C:phagophore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017128">
        <property type="term" value="F:phospholipid scramblase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034497">
        <property type="term" value="P:protein localization to phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007241">
        <property type="entry name" value="Autophagy-rel_prot_9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13038">
        <property type="entry name" value="APG9 AUTOPHAGY 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13038:SF10">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04109">
        <property type="entry name" value="ATG9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 19" id="KW-0072">Autophagy</keyword>
    <keyword evidence="14" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12 19" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="13 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7 19" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="448"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="537"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="569"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="637"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="24"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="123"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="748"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="65"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="166"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="754"/>
            <end position="782"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006185"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00018074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
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            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00024479"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00024615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00024621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00024631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU364027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="CAZ79631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="898" mass="102754" checksum="38919654CFB12784" modified="2010-06-15" version="1">MTSRILSQIFNQNSASIYETLRQHDATDNSDDDDDLEERAGMLALDNGSHLPPLDIQDGLEDSNFELQQQPPAPSESSFMASENNRRSYFSSMSRSKKNIYAPPRYRDEEEGDDEVPASLLLETGAGPSKRRGSTGGRSGGVVPAPRATRNVLLPNTWIATGLEGHQAREERRADRRQQSEGKDFRRQARLGLIDPKERALWKWANVENLDNFLNDVYDYFLGKGIWSIALHRSLNQLTLAFVVGFTTFMSRCVDYTLVPERKKLSEVIIPHCMSRMSGVTTLFMWAISFYWVIKTIHYILDTRRLWDIRNFYKYLLEIPDSDMQTVTWQEVVAKLMALRDSNPTTSGALRRRHFSSQSKQRMDAHDIANRLMRKENYLIALFNKEILDLTIPFPFLRSRGTMLTKTLEWNLSLCILDYVFNDQGQLRTLFLKESHRKILSDGLKQRFLFAGVMNIIFAPFILTYLLLLFLLRNVNDFIKTPARIGHRQYTPLAEWKFREFNELYHIFHMRLNMSYPAAEAYVEQFPKEKTAQVARFVSFVAGSLLGVLAIASIFDGDLIQGFEIVQGMNGLTAIATLTTIVAVSRGMLPEENTVYDPEWSIRNVIQHIHYMPDHWKDKLRTDDIKKEFMSLYDLKVTIFLGEVMSVIFTPFVLWVSLSNRSERIIDFFREFTVHVDGIGYVCSFAVFDFQKPGRQSMADIREEYFSSKDNKMLSSYLGFMDQYHGPQNKGGANKRQMSMRNYMSPMLNAQTGKGKDGRGAQDRRAGDGRQDMQQSIHRDNPGRMQGSQILQSSLLDQHHQPPPFVARAPRHPAFHLSTAESLREEEEDAIASADKPREHYRSNLGESFMSTRVTGTMMAAQAEDDEEDQQAGPHNAGVLDLLNQFVGVEGAGRTGVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5G555</accession>
    <name>D5G555_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00000261001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">tRNA nucleus export receptor which facilitates tRNA translocation across the nuclear pore complex. Involved in pre-tRNA splicing, probably by affecting the interaction of pre-tRNA with splicing endonuclease.</text>
    </comment>
    <dbReference type="EMBL" id="FN429996">
        <property type="protein sequence ID" value="CAZ79640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835483.1">
        <property type="nucleotide sequence ID" value="XM_002835437.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G555"/>
    <dbReference type="FunCoup" id="D5G555">
        <property type="interactions" value="745"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G555"/>
    <dbReference type="GeneID" id="9186338"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000261001"/>
    <dbReference type="eggNOG" id="KOG2020">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003712_0_0_1"/>
    <dbReference type="InParanoid" id="D5G555"/>
    <dbReference type="OMA" id="IAKRSWG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042565">
        <property type="term" value="C:RNA nuclear export complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005049">
        <property type="term" value="F:nuclear export signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006405">
        <property type="term" value="P:RNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013598">
        <property type="entry name" value="Exportin-1/Importin-b-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045478">
        <property type="entry name" value="Exportin-5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045065">
        <property type="entry name" value="XPO1/5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11223">
        <property type="entry name" value="EXPORTIN 1/5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11223:SF3">
        <property type="entry name" value="EXPORTIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19273">
        <property type="entry name" value="Exportin-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08389">
        <property type="entry name" value="Xpo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Exportin-1/Importin-beta-like" evidence="4">
        <location>
            <begin position="132"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Exportin-5 C-terminal" evidence="5">
        <location>
            <begin position="359"/>
            <end position="1203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1212"/>
            <end position="1249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1222"/>
            <end position="1239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00025147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF19273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ79640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1249" mass="140419" checksum="86A5C05137E3B8F2" modified="2010-06-15" version="1">MNGGGVPGDAVGSPTTNGGGPANGRALQQPQPLDQIKQALQIVYDPHSSNDSRKQAGAFLESAKSDKEAPYHGFTLACDRGHDAVVRHFGLLLLEQAVRFSWVDYTAEESGMVRGWVLSLAENVKIDGRDPVYLRNKIAQLWVEVAKRSWASEWLDMDELLVRLWDNSTQQGNPAQRDLVLSILENLVEDVFNREDSVAGIRNTPLSKACIDIFTPLDVLKENFPGREPSAVLRCGEGGWLTRLTEMLEKCLEAGVNGNTEVEACAVKVLGTLKACMSWAIPKAIAAAKIVETVGRALMVEKISVQMVAVECLHTLFTRIFFEDDDFQKIVCPMYYSQTVELLANIFDWIRVDATDIDEERYLFLKRYAEMIANLGGFVEEKSGLMPVEADLVGFMKLLFQICSHDSLSVSIPVMNAWTRIIRKDLLSGHEAIGPLIPGLIALATARLIRYESVTDESNRSIIFLNEDLDTMPDRHAFLGNYRRFCQSIVECLVRKSPFDIFPYILAQADASLSDLCSKEPPFTAETYRKNSGPFLQLDAHFTVVEASLRGYMKWIDTIGKNPTNNNSRNELEQRLTEWCERVLSMNFGDPMIRRRVIQLMVTVSTSALDKKADLMLKILEHVLLTRPPEYPANHAYTDAVKELMGVCTTEIQRLAMKMPDHLMLVYEGLQTKINEIISTENVDDRTRIAFHTFLFTINHRTKNIDPAIRQSRLESYIHPVRETWRDPGLTESLQTFQGFCTILGLDKVQEYLISRRVNEIPDFTKHVLDTEGQALQNELAEKFKALPIRATKAFLAVSTEQLRKPSNAYNIACALWHESIPMILPNLLQFLTHAHAFHNAGNWAGLQPELQAVIRKILTDRFWQAGISTGSRDEFYENVTKTKTTMEGLASSIRGAIRMVREACYSILWCMSRLDVHFYGLNELPGPLALALFADAHALSSHQMSILLNMTRYIIDDCPTTLRAHFLTPLLASLFTQVDRKISGEWDELIRKGQISLGEDKLAEEMKEESILRQLTYTAVLIVAGLLDPQRPGETVNTNSGFLDQLALPEMQAAGPGEQTMREFILSSDCVLEPLILFCTHALRMRDSRCCGIIIRVFRSIVPEFQADRADIREFICREVFIAAIEALHTDYFVDVQKDLAQLIAAIFTLYSPTSGTPRSLLLNLPGLTEAKVDACYKKLTAAGSTRQQRALILELLDGLRGVAISEKGKLQRPQVGKQRSQAEKRQQQQYAEKRESPDLGGVADMFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5G556</accession>
    <name>D5G556_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00000262001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429996">
        <property type="protein sequence ID" value="CAZ79641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835484.1">
        <property type="nucleotide sequence ID" value="XM_002835438.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G556"/>
    <dbReference type="GeneID" id="9186339"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000262001"/>
    <dbReference type="HOGENOM" id="CLU_2559954_0_0_1"/>
    <dbReference type="InParanoid" id="D5G556"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ79641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="82" mass="9276" checksum="F0D91042C2E4EBD1" modified="2010-06-15" version="1">MDRRIEASGWVCWYAFPPTDITISARGPLHYDSRKQCYLGANDISKSVNITTTRPSKTRITNSELILAMCSSTFKGLRYSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5G3</accession>
    <name>D5G5G3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00004322001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835599.1">
        <property type="nucleotide sequence ID" value="XM_002835553.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5G3"/>
    <dbReference type="STRING" id="656061.D5G5G3"/>
    <dbReference type="GeneID" id="9183742"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004322001"/>
    <dbReference type="eggNOG" id="ENOG502S320">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_680059_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5G3"/>
    <dbReference type="OMA" id="DIAGNDM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="203"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="289"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="362"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="405" mass="43908" checksum="AA4855DA143CAF97" modified="2010-06-15" version="1">MPHDIAGNDMPVPEPDPFGSGDHDAYDLSSTGNYPSPPGLAPLSGLLGYSEANRDFFVRTSLTQTTALLGLKLTPAEREGLTYYNSRFYSYESYGDVLGISAGILISFLLRNRKPGVISTTMVRVFTKPGAQRDNAAKWLSRGLKLSVWGCVGRMYGLTSAGFQALQKNKAEKRLDPNLRRVVAAANKKKEALVHEIQANGPRLRRMEGLENPGAQDDGQSLGGMEISDGTEDRLDASEVFPHGRPSEQVAELEARKRSGGHPGNDPFGVDDASPTRGDGVMGQGQGGWTNTHSNTSESAWERIRKGQSPTTRPPTDRGEGDGNTTYTSEPKVGGWPGRASMKGYGQGANREPGTDSFTFSKSEEERELAKQEAQKEFDAKVEKERSGGSGDGYTEGDSRNRRWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5G7</accession>
    <name>D5G5G7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00004327001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="CAZ79760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002835557.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5G7"/>
    <dbReference type="STRING" id="656061.D5G5G7"/>
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    <dbReference type="KEGG" id="tml:GSTUM_00004327001"/>
    <dbReference type="eggNOG" id="ENOG502S0EA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054794_1_0_1"/>
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    <dbReference type="OMA" id="RITLMWC"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011576">
        <property type="entry name" value="Pyridox_Oxase_N"/>
    </dbReference>
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        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39336">
        <property type="entry name" value="PYRIDOXAMINE PHOSPHATE OXIDASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_6G11440)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39336:SF1">
        <property type="entry name" value="PYRIDOXAMINE PHOSPHATE OXIDASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_6G11440)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PNPOx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="240"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridoxamine 5'-phosphate oxidase N-terminal" evidence="2">
        <location>
            <begin position="9"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ79760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="262" mass="28493" checksum="6CAC066940D18F41" modified="2010-06-15" version="1">MPTFYPSLTEDLITWLQTQPLFFTASAPRYGKHVNLSPKGYTSRSFRVLSPNQAAYLDATGSGSETISHIYENGRVTVMFTSFSASPRILRLFCTGRVVEAENAEFDDIVQSFGPRGEEEGGEGKGGMKGARAVVLLDIFKVQTSCGYGVPIAEGAPTGGEEGGGGVEGEARFGERETMERWAEKQIEKGNLETYRAEWNSSSLDGLPGLRSARREKGERALWVGDLLARAKRVGREWEGLLVGLGVGVAVGFGLQRYLVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5L1</accession>
    <name>D5G5L1_TUBMM</name>
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        <recommendedName>
            <fullName evidence="8">Ubiquitin-activating enzyme E1-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">GSTUM_00004395001</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">Mel28</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1 8">Protein modification; protein sumoylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ubiquitin-activating E1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835647.1">
        <property type="nucleotide sequence ID" value="XM_002835601.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5L1"/>
    <dbReference type="FunCoup" id="D5G5L1">
        <property type="interactions" value="1661"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G5L1"/>
    <dbReference type="GeneID" id="9183790"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004395001"/>
    <dbReference type="eggNOG" id="KOG2013">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013325_7_3_1"/>
    <dbReference type="InParanoid" id="D5G5L1"/>
    <dbReference type="OMA" id="TPSEHIH"/>
    <dbReference type="UniPathway" id="UPA00886"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031510">
        <property type="term" value="C:SUMO activating enzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019948">
        <property type="term" value="F:SUMO activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01489">
        <property type="entry name" value="Uba2_SUMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.80:FF:000002">
        <property type="entry name" value="SUMO-activating enzyme subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.520">
        <property type="entry name" value="Ubiquitin activating enzymes (Uba3). Chain: B, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.80">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, inactive adenylation domain, subdomain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.20">
        <property type="entry name" value="Ubiquitin-like 2 activating enzyme e1b. Chain: B, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028077">
        <property type="entry name" value="UAE_UbL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042449">
        <property type="entry name" value="Ub-E1_IAD_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023318">
        <property type="entry name" value="Ub_act_enz_dom_a_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030661">
        <property type="entry name" value="Uba2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019572">
        <property type="entry name" value="UBA_E1_SCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033127">
        <property type="entry name" value="UBQ-activ_enz_E1_Cys_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF5">
        <property type="entry name" value="SUMO-ACTIVATING ENZYME SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14732">
        <property type="entry name" value="UAE_UbL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10585">
        <property type="entry name" value="UBA_E1_SCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039133">
        <property type="entry name" value="SUMO_E1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00865">
        <property type="entry name" value="UBIQUITIN_ACTIVAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="6 8 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="14">
        <location>
            <begin position="1"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-activating enzyme SCCH" evidence="15">
        <location>
            <begin position="271"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin/SUMO-activating enzyme ubiquitin-like" evidence="16">
        <location>
            <begin position="420"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="520"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="549"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="529"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="9 12">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="3"/>
            <end position="8"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="35"/>
            <end position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="96"/>
            <end position="101"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="409"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="412"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR039133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR039133-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR039133-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR039133-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF10585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF14732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAZ79804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="605" mass="67075" checksum="0E4147A2E94D2AEF" modified="2010-06-15" version="1">MVGAGGIGCELLKNLVLTGFGEIHIVDMDTIDLSNLNRQFLFGMQHIKKSKAMVAKETAGKFNPNVKLEAHHANIKDPEFNVKWFGGFNVVFNALDNLEARRHVNKMCLSADVPLIESGTTGFDGQVQVIKRGKTECYDCTHKATPKSFPVCTIRSTPSQPIHCIVWAKSYLLVEIFGTSEESSPDLDPTEDSENSREIANLKREAEELKNIREKMDSPDFAATVFNKVFRDDINRLRSMDEMWKSRKPPEALDHQMVSDEAKGIDAKVSENDQKIWSLVENYIMFEDSLRRLAERVLDMKRDQVGDEAAPIITFDKDDEDTLDFVAASANLRSLVFGINVKSKFDIKQMAGNIIPAIATTNAITAGLCVLQAFKVLRDDFTSARTIFLSRLADRAFCTEKPSPPNPTCGVCSVARTAIEADIERATLGDLLDNVLRDGLRYGGEITVMTSQLLYDCDFDDNIDRTLKGLGFSEETFVTVVDDEDDPRINLEVLVTNTQLPEDSTPITIPAYFEIPKKSSAKLETPEPSSLNGNSANGSATKRSIEEVIGSDELDQSNGPDTKKLRRDIVVEDRVPASAKKLGKMPATEMDDIVVIDDDGTITID</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5R4</accession>
    <name>D5G5R4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">Fructose-bisphosphate aldolase</fullName>
            <shortName evidence="13">FBP aldolase</shortName>
            <ecNumber evidence="5 13">4.1.2.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00001447001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 13">Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>beta-D-fructose 1,6-bisphosphate = D-glyceraldehyde 3-phosphate + dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:14729"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.1.2.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12 13">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="12 13">Binds 2 Zn(2+) ions per subunit. One is catalytic and the other provides a structural contribution.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 13">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the class II fructose-bisphosphate aldolase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.13" evidence="5 13"/>
    <dbReference type="EMBL" id="FN430001">
        <property type="protein sequence ID" value="CAZ79857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835700.1">
        <property type="nucleotide sequence ID" value="XM_002835654.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5R4"/>
    <dbReference type="FunCoup" id="D5G5R4">
        <property type="interactions" value="653"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G5R4"/>
    <dbReference type="GeneID" id="9187535"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001447001"/>
    <dbReference type="eggNOG" id="KOG4153">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036923_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5R4"/>
    <dbReference type="OMA" id="PRTWGKL"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00183"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004332">
        <property type="term" value="F:fructose-bisphosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00946">
        <property type="entry name" value="FBP_aldolase_IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000013">
        <property type="entry name" value="Class II fructose-bisphosphate aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000771">
        <property type="entry name" value="FBA_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006411">
        <property type="entry name" value="Fruct_bisP_bact"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00167">
        <property type="entry name" value="cbbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01520">
        <property type="entry name" value="FruBisAldo_II_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006628">
        <property type="entry name" value="PRK09197.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30559:SF0">
        <property type="entry name" value="FRUCTOSE-BISPHOSPHATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30559">
        <property type="entry name" value="FRUCTOSE-BISPHOSPHATE ALDOLASE CLASS 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01116">
        <property type="entry name" value="F_bP_aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001359">
        <property type="entry name" value="F_bP_aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00602">
        <property type="entry name" value="ALDOLASE_CLASS_II_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00806">
        <property type="entry name" value="ALDOLASE_CLASS_II_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="9 13" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 12 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12 13" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>dihydroxyacetone phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="331"/>
            <end position="333"/>
        </location>
        <ligand>
            <name>dihydroxyacetone phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="352"/>
            <end position="355"/>
        </location>
        <ligand>
            <name>dihydroxyacetone phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001359-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001359-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR001359-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU366023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ79857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="424" mass="46827" checksum="BF7F106D588ADFDB" modified="2010-06-15" version="1">MTPHHSSRARFQAFPTQLQKEHFPHQQLFLPLPYIISLFLYSSPVNTLSLKIFLHLPLKLKSNLSLIDQSASQKSGVIVGDDVLNLFKYAKEHKFAIPAINVTSSSTVVASLEAARDAKSPLILQMSQGGAAYFAGKGVSNTDQFASISGAIAGANYIRSIAPAYGIPVVLHTDHCAKKLLPWLDGMLDADEKHFAEHGEPLFSSHMIDLSEEDKEWNISTTKKYLQRAAPMSQWLEMEIGITGGEEDGVDNTGVDNSSLYTQPEDIYQVYEALSSVSPYFSIAAAFGNVHGVYKPGNVKLHPELLGKHQAYAKEKINCEEDKPLFLVFHGGSGSSKEEFSAGISHGVVKVNLDTDLQYAYLTGIRDYVLNKKDFIASQVGNPEGEDKPNKKYFDPRVWVREGEKTMSARVKEALTDFNTANQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5S7</accession>
    <name>D5G5S7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00001462001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430001">
        <property type="protein sequence ID" value="CAZ79870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835713.1">
        <property type="nucleotide sequence ID" value="XM_002835667.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5S7"/>
    <dbReference type="FunCoup" id="D5G5S7">
        <property type="interactions" value="103"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G5S7"/>
    <dbReference type="GeneID" id="9187548"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001462001"/>
    <dbReference type="eggNOG" id="ENOG502SDW5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122989_1_0_1"/>
    <dbReference type="InParanoid" id="D5G5S7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019711">
        <property type="entry name" value="ATP_synth_F0_suH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28207">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT H, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28207:SF1">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT H, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10775">
        <property type="entry name" value="ATP_sub_h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="9"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="87" mass="9499" checksum="2BE89871D1D7371F" modified="2010-06-15" version="1">MYISALKSYKPAAAKATDSEGQVKKWAPPAAPQSPEAAEASLAQELKAYETQEVEIEGQSPEPGMEAQEEDWFEEEANFGVEESAKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>D5G612</accession>
    <name>D5G612_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00001720001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430005">
        <property type="protein sequence ID" value="CAZ79955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835798.1">
        <property type="nucleotide sequence ID" value="XM_002835752.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G612"/>
    <dbReference type="STRING" id="656061.D5G612"/>
    <dbReference type="GeneID" id="9187633"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001720001"/>
    <dbReference type="eggNOG" id="ENOG502QSY9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007426_0_2_1"/>
    <dbReference type="InParanoid" id="D5G612"/>
    <dbReference type="OMA" id="EPPEWRG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050613">
        <property type="entry name" value="Sec_Metabolite_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001">
        <property type="entry name" value="UNCHARACTERIZED TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001:SF49">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="5">
        <location>
            <begin position="36"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="51"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="108"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="706"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="855"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="7"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="63"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="81"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="148"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="729"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="864"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="886"/>
            <end position="897"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ79955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="923" mass="101496" checksum="7BDEB86EB61C5A1E" modified="2010-06-15" version="1">MAPTPPSSSGHSPQDANTQSSKEFRVVKKRNRVPLSCAPCRNRKLKCNRSHPCENCTRRGDPSTCTYASTPNGPSRARKQSAGSNGFSSDEMQSRIDRLEGLVLRLMGSGGNHVSSPPDRETSASTDDGTNKNKCCDDDDDVGMGSVHGDEKRRGEGESDEDVEEVRSALGIMKVDQGKSYYRGETHWAAILSEISEVKNFFNEVKQNWEEKLAHMKAKNAGPNKLDRTGFPFSAGKLPSRQELLSLVPRRELVDLLVDGFFRNYDPIFHVLHRPTFYKQYEDFWRDPTQVDLIWLGLLMAIMCLSLKIYHYSGEEPPEISGKTQEMSYAFRLATEQCLIVGEFTKKMYIHTVQALILVCTWQTSKDHVDESWLFQGLLVRIAMCMGLHRDPMQFGSTITPSEAEIRRRIWTMICCMDVLESIKIGLPSMIRQEECDTRLPMNLHDDEVGEGVDVLPPERPREELTGVSYMIAKASMSKVYSRIVAQTSSLEPRPAYDQILKMDAEIREMHAAVPPFLRLKPTEECKDDPAWLIIQRYNLDLIAHKALATLHRPYAARAEHNPRYEGSRQKCVEAALVLLRHQAEIILDTQSTLKHARWFTEGLGCHDFLHAAMIICLDLSTQCRIARDRSKTQGEQGFLLGARERAEQYNVLEKARLIYEKQRDTSIDATKAYGVLTVMLDKIRGGPGYFDFSLGCPIEARKAVEKNGAAPTPQGSIPPITGGTPVFATPSPSSSEAPQAPPNPAKSDTAAMSLGLVGQSVLSPNAAAAVLYSARTPGGHNVIMDDASNTEYGASPSESSSMSQFWPQSGVDIPSGLDWNEWDNFMQGINLDSNQSPAWPMMTALPGPPQQPLLNPDGTYRVSPSSAATPTNTAPASTSTAPGDATGGGGGGGGQVPAGSGLPTSTTGYFVTEGMDIGSLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6B0</accession>
    <name>D5G6B0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00001682001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430008">
        <property type="protein sequence ID" value="CAZ80053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835896.1">
        <property type="nucleotide sequence ID" value="XM_002835850.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6B0"/>
    <dbReference type="FunCoup" id="D5G6B0">
        <property type="interactions" value="619"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G6B0"/>
    <dbReference type="GeneID" id="9187724"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001682001"/>
    <dbReference type="eggNOG" id="KOG0010">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024293_0_1_1"/>
    <dbReference type="InParanoid" id="D5G6B0"/>
    <dbReference type="OMA" id="PGMDMFG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036435">
        <property type="term" value="F:K48-linked polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072665">
        <property type="term" value="P:protein localization to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030474">
        <property type="term" value="P:spindle pole body duplication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14324">
        <property type="entry name" value="UBA_Dsk2p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16106">
        <property type="entry name" value="Ubl_Dsk2p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000024">
        <property type="entry name" value="Ubiquitin domain-containing protein DSK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006636">
        <property type="entry name" value="STI1_HS-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015496">
        <property type="entry name" value="Ubiquilin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10677:SF3">
        <property type="entry name" value="FI07626P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10677">
        <property type="entry name" value="UBIQUILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00727">
        <property type="entry name" value="STI1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="3">
        <location>
            <begin position="21"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="2">
        <location>
            <begin position="410"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="97"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="255"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="303"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ80053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="454" mass="45613" checksum="67E3BAAF9132440B" modified="2010-06-15" version="1">MSSSEQSAQGPPPSSESSVPITFSVKSSNDTKYTVELPPTTSVADLKTTLAGMSDIAADRQRLIYSGRVMKDEETLAFYKLQSGHTVHMVKGAASNTARGGASSSGPTTGGADARPAVPTNIAAGTGNNPLAGLTGARYAGHVQLPNADMFGPDGGMGPPPDAEQLAQAMSNPQFQSTMNDLLSNPQIMDYIFASNPMLSQLGPEVRQMMQSDLFRQLMSNPQVIRSMSQMTGGLGRGFGGMGGAAASFPEPGRTDTTPSSDSPAGSTPAESTPNPAAAGSPPPLNPFLPGAAGGNPFAALFGGQPPRAGAPGASPSQTPGQAPGQVPGGLDANAINSILGALGGGGGGQPSASTGDPASGAPNQPLPPNHPLAGNNPLAILLGANGPLGGAGALRAAQAQAAAPVDNRPPEERYAEQLGQLNDMGFYDFDRNVAALRRSGGSVQGAINELLDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6X7</accession>
    <name>D5G6X7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00002375001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430019">
        <property type="protein sequence ID" value="CAZ80270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842535.1">
        <property type="nucleotide sequence ID" value="XM_002842489.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6X7"/>
    <dbReference type="GeneID" id="9184084"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002375001"/>
    <dbReference type="HOGENOM" id="CLU_2887455_0_0_1"/>
    <dbReference type="InParanoid" id="D5G6X7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="63" mass="6779" checksum="97723BBA0AA13D74" modified="2010-06-15" version="1">MDVYILSALRYLHGVGIINTGTGARRPTDSETKNFVGWNSGGPTFVRSSPTGEGKYDTVGNCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5G763</accession>
    <name>D5G763_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA-directed RNA polymerase II subunit RPB3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00002358001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the archaeal Rpo3/eukaryotic RPB3 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430021">
        <property type="protein sequence ID" value="CAZ80356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836165.1">
        <property type="nucleotide sequence ID" value="XM_002836119.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G763"/>
    <dbReference type="FunCoup" id="D5G763">
        <property type="interactions" value="955"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G763"/>
    <dbReference type="GeneID" id="9185254"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002358001"/>
    <dbReference type="eggNOG" id="KOG1522">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038421_1_1_1"/>
    <dbReference type="InParanoid" id="D5G763"/>
    <dbReference type="OMA" id="FYFEVES"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07031">
        <property type="entry name" value="RNAP_II_RPB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.120.12:FF:000002">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.120.12">
        <property type="entry name" value="DNA-directed RNA polymerase, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="entry name" value="RNA polymerase, RBP11-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00320">
        <property type="entry name" value="RNApol_arch_Rpo3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011262">
        <property type="entry name" value="DNA-dir_RNA_pol_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011263">
        <property type="entry name" value="DNA-dir_RNA_pol_RpoA/D/Rpb3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022842">
        <property type="entry name" value="RNAP_Rpo3/Rpb3/RPAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036643">
        <property type="entry name" value="RNApol_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050518">
        <property type="entry name" value="Rpo3/RPB3_RNA_Pol_subunit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001988">
        <property type="entry name" value="PRK00783.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11800">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11800:SF2">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01000">
        <property type="entry name" value="RNA_pol_A_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01193">
        <property type="entry name" value="RNA_pol_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00662">
        <property type="entry name" value="RPOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56553">
        <property type="entry name" value="Insert subdomain of RNA polymerase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="RBP11-like subunits of RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase RpoA/D/Rpb3-type" evidence="8">
        <location>
            <begin position="25"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00072506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ80356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="340" mass="37290" checksum="833F9BD5128CAF95" modified="2010-06-15" version="1">MDDYDTIYGSDRGPQITIKEADSRHAQIVLSNCDLAFANALRRIMLAEIPTIAIDIVEIQTNTSVIPDEFLCHRLGMVPLDSTNIDDKLIYTRDCFCDQYCERCSVTLSLHARCTGEDNVTVYARDLVIAEGAQLGSPVINDPEGQGTVICKLRKGQELRLKCIAKKGIAKEHAKWAPTAAVGFEYDPWNKLRHIDYWYEEDAKEEWPKSKYADWEEPPIEGEPFNYDAVPSRFYLDIESAGSLPPDEILQQGIKYLQEKLAAVIETFSNKNETGDGFGDGVRSPGQDYSMGGMGGGGAGYETPYQGGRTPFGGGGFGGATEYGGRLMGGHKCVAAVAMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5G772</accession>
    <name>D5G772_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00002495001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430022">
        <property type="protein sequence ID" value="CAZ80365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836174.1">
        <property type="nucleotide sequence ID" value="XM_002836128.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G772"/>
    <dbReference type="STRING" id="656061.D5G772"/>
    <dbReference type="GeneID" id="9185263"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002495001"/>
    <dbReference type="eggNOG" id="KOG0055">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_814297_0_0_1"/>
    <dbReference type="InParanoid" id="D5G772"/>
    <dbReference type="OMA" id="NILTIMM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18577">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="74"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="117"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="190"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="214"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="291"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="76"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ80365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="341" mass="36936" checksum="A7306500AA26DE44" modified="2010-06-15" version="1">MASFPGANQPLSRRRSHHSIDIASLNPSAPTSPNTTPDPAPPAIPVLEQLLGFKSRTSYFSLYRPLNTWHQRTVLALAIILSLAAGAPLPIIGVIFGKIINHFPPDEDELQTRICQLLGVSVAYFFTTWGWATCWGIVGERVSRGLREDLLKKAVGMEISYFEIECPDIANRLTADTQTIHLGTSEKAGLFLQSVSYFIAAFTAGFFLNARLTGILFAAVVPAMLIVITFGSKVVAKYAREATEYSEKATSVAEGAIIAVQVVQAFDALDKLADEHLKFMRPAIMKGASKAFAGALMLGLVYFVAYSANALAFWQGSQQIAEGSFKEDEGAGTVYTVVPSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7A9</accession>
    <name>D5G7A9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00002419001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430023">
        <property type="protein sequence ID" value="CAZ80402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836211.1">
        <property type="nucleotide sequence ID" value="XM_002836165.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7A9"/>
    <dbReference type="GeneID" id="9185300"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002419001"/>
    <dbReference type="HOGENOM" id="CLU_2905792_0_0_1"/>
    <dbReference type="InParanoid" id="D5G7A9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="62" mass="7159" checksum="89375E95EC4729EA" modified="2010-06-15" version="1">MIPAPPINNKNHTEPVRLITTPRRISHQPFTPSLQRIGEKGKKTKKHTQWKSSPFLPYLSTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7E3</accession>
    <name>D5G7E3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00002191001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HpcH/HpaI aldolase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430025">
        <property type="protein sequence ID" value="CAZ80436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836245.1">
        <property type="nucleotide sequence ID" value="XM_002836199.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7E3"/>
    <dbReference type="STRING" id="656061.D5G7E3"/>
    <dbReference type="GeneID" id="9188461"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002191001"/>
    <dbReference type="eggNOG" id="ENOG502QR7H">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1918594_0_0_1"/>
    <dbReference type="InParanoid" id="D5G7E3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016832">
        <property type="term" value="F:aldehyde-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005000">
        <property type="entry name" value="Aldolase/citrate-lyase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050251">
        <property type="entry name" value="HpcH-HpaI_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30502">
        <property type="entry name" value="2-KETO-3-DEOXY-L-RHAMNONATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30502:SF0">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYLASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03328">
        <property type="entry name" value="HpcH_HpaI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HpcH/HpaI aldolase/citrate lyase" evidence="4">
        <location>
            <begin position="17"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ80436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="132" mass="13599" checksum="DFAC16B150381128" modified="2010-06-15" version="1">MAELTTGQCTKPSAGVAGCGVSPIVRIAACEAWVFKRALDAGAHGAMVPFVSTAEEAKAALNYANPFPMGAFNASSSIEYLKSANYSILTIDQIETKQALDNVDAIAGVEGVDVLFVGPFDLGNSLGCPIIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7R0</accession>
    <name>D5G7R0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">D-lactate dehydratase</fullName>
            <ecNumber evidence="1">4.2.1.130</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00004685001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>methylglyoxal + H2O = (R)-lactate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:27754"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16004"/>
            <dbReference type="ChEBI" id="CHEBI:17158"/>
            <dbReference type="EC" id="4.2.1.130"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="4.2.1.130" evidence="1"/>
    <dbReference type="EMBL" id="FN430031">
        <property type="protein sequence ID" value="CAZ80553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836362.1">
        <property type="nucleotide sequence ID" value="XM_002836316.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7R0"/>
    <dbReference type="STRING" id="656061.D5G7R0"/>
    <dbReference type="GeneID" id="9187032"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004685001"/>
    <dbReference type="eggNOG" id="KOG2764">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_44_2_1"/>
    <dbReference type="InParanoid" id="D5G7R0"/>
    <dbReference type="OMA" id="AFCAGPT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019172">
        <property type="term" value="F:glyoxalase III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903189">
        <property type="term" value="P:glyoxal metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002818">
        <property type="entry name" value="DJ-1/PfpI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050325">
        <property type="entry name" value="Prot/Nucl_acid_deglycase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48094:SF12">
        <property type="entry name" value="PARKINSON DISEASE PROTEIN 7 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48094">
        <property type="entry name" value="PROTEIN/NUCLEIC ACID DEGLYCASE DJ-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01965">
        <property type="entry name" value="DJ-1_PfpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DJ-1/PfpI" evidence="3">
        <location>
            <begin position="11"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00048082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ80553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="156" mass="16807" checksum="50C45118263D9151" modified="2010-06-15" version="1">MVILFFNRFVLSRGVKIVPDVQSLDPEAQLPDLIVLPGGAPGAKTFEESDAVARLIDRARKEGVYIGAICAATKALVRFGAEGGWKARVTSHPSVQEAVVKAGWEYAQDKVVLDGKVITSRGPGTSLLFALTLVETLCGRERREEVEAPMITASEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7T9</accession>
    <name>D5G7T9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glucosamine 6-phosphate N-acetyltransferase</fullName>
            <ecNumber evidence="8">2.3.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00004721001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>D-glucosamine 6-phosphate + acetyl-CoA = N-acetyl-D-glucosamine 6-phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10292"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57513"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="EC" id="2.3.1.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route I): step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the acetyltransferase family. GNA1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.4" evidence="8"/>
    <dbReference type="EMBL" id="FN430031">
        <property type="protein sequence ID" value="CAZ80582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836391.1">
        <property type="nucleotide sequence ID" value="XM_002836345.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7T9"/>
    <dbReference type="FunCoup" id="D5G7T9">
        <property type="interactions" value="419"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G7T9"/>
    <dbReference type="GeneID" id="9181401"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004721001"/>
    <dbReference type="eggNOG" id="KOG3396">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072095_0_1_1"/>
    <dbReference type="InParanoid" id="D5G7T9"/>
    <dbReference type="OMA" id="NQRYDWI"/>
    <dbReference type="UniPathway" id="UPA00113">
        <property type="reaction ID" value="UER00529"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004343">
        <property type="term" value="F:glucosamine 6-phosphate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000048">
        <property type="entry name" value="Glucosamine 6-phosphate N-acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039143">
        <property type="entry name" value="GNPNAT1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13355">
        <property type="entry name" value="GLUCOSAMINE 6-PHOSPHATE N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13355:SF11">
        <property type="entry name" value="GLUCOSAMINE 6-PHOSPHATE N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="9">
        <location>
            <begin position="23"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ80582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="167" mass="18504" checksum="9F853AED3354841B" modified="2010-06-15" version="1">MATPLFSKDLISPSVTSELPKGYNLRPLQKSDHAEFLSVLTVLTKVGDISLETWSARYDWMAARNDEYFIVVIENELGKVVAVGSLIIEKKFIRNCAAVGHIEDIAVAADQQGKKLGLRIIQALDAIAQQVGCYKSILDCSEKNQGFYEKCGFKLAGVQMAHYYEGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8L6</accession>
    <name>D5G8L6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00003001001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430047">
        <property type="protein sequence ID" value="CAZ80859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836668.1">
        <property type="nucleotide sequence ID" value="XM_002836622.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8L6"/>
    <dbReference type="FunCoup" id="D5G8L6">
        <property type="interactions" value="697"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G8L6"/>
    <dbReference type="GeneID" id="9186227"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003001001"/>
    <dbReference type="eggNOG" id="KOG0573">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012368_2_0_1"/>
    <dbReference type="InParanoid" id="D5G8L6"/>
    <dbReference type="OMA" id="SVYESCP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004066">
        <property type="term" value="F:asparagine synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006529">
        <property type="term" value="P:asparagine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01991">
        <property type="entry name" value="Asn_synthase_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001962">
        <property type="entry name" value="Asn_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051857">
        <property type="entry name" value="Asn_synthetase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45937">
        <property type="entry name" value="ASPARAGINE SYNTHETASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45937:SF1">
        <property type="entry name" value="ASPARAGINE SYNTHETASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00733">
        <property type="entry name" value="Asn_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0061">Asparagine biosynthesis</keyword>
    <keyword evidence="3" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Asparagine synthetase" evidence="5">
        <location>
            <begin position="446"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="302"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="302"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ80859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="559" mass="61386" checksum="F6413A405FF1198D" modified="2010-06-15" version="1">MCGILFSITSTTSPPLHPTSTSLPDLISRRGPDTTNTHVTAFENYALTFTSSVLSLRGRLTPQPLVDPVTGSVLCWNGEAWRINSALVPAGVNDGEEVLKLLLSAETPEKGFAGIAGPWACVFFDAVRGRVWYGRDCLGRRSLLELRKGGWKDGVTIASVGDGHEGWKEVEAYGLRYFSLQSGEEGVVPFLHREDRDGGQVSMELPFPKLNRNIEPDGRLNTDSPEVGKLKEVLLGSVRIRVGDIPEPPGPGRKVRLAVLFSGGLDCSVIARLVHECLPLDEAVDLLNVAFENRRFVEARNKANKANKPKRRTQHPEAAAPPEDPQREGAVDPYSICPDRITGLATYDELQKVCPGRLWNFIQINVPYTDLLSHRQSVINLIHPHNTEMDLSIGVAFYFASRGTGYSFPGNQPYSTPARILLSGLGADELLGGYARHGTAFRSRSYAGLLEELQLDVGRLGKRNLGRDDRVLAHWGREARYPFLDEDVVAWCLSTGVIRKCGGSEEEVVEGKRVLRLLAGRLGMQEVAREKKRAVQFGSRSARMEAGEKGRVKGTAVVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8W5</accession>
    <name>D5G8W5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00004868001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC35F solute transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430053">
        <property type="protein sequence ID" value="CAZ80958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836767.1">
        <property type="nucleotide sequence ID" value="XM_002836721.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8W5"/>
    <dbReference type="GeneID" id="9181241"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004868001"/>
    <dbReference type="eggNOG" id="KOG2766">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039639_4_1_1"/>
    <dbReference type="InParanoid" id="D5G8W5"/>
    <dbReference type="OMA" id="VRYHWAQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009262">
        <property type="entry name" value="SLC35_F1/F2/F6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052221">
        <property type="entry name" value="SLC35F_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14233">
        <property type="entry name" value="DUF914-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14233:SF4">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER F2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06027">
        <property type="entry name" value="SLC35F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="56"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="153"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="181"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="217"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="248"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="278"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="306"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="333"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ80958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="410" mass="45173" checksum="C8878DD899612160" modified="2010-06-15" version="1">MEETRKGHPEPTVCQQAEDPYAHRGTVPANTDDTAEQAFVPPVKEGRFAFLKTRRFYEVLVIGQILSLCLVSTGTLSLLLVGQGTSIPAFQSFFSYVLLNLVYTSYTLHQYGFKKWAQVVLKDGWKYFILSFLDVEGNYFVVLAYRYTTILSAQLINFWAIVVVVILSFIFLHVRYRIPQILGILICCGGMGVLLASDAMGGVAITGMPTELKGDLFMLLGATMYGISNVLEEFFVSKKPIFEVIGQLAFWGMLINGTQAGIFDRASFRSATWNGKVAGYMVGYTLSLFIFYSIVPILLRMASAAFFNISILTTSFWSVLIGIRVFGYVIRKLYPAAFVMIILGLVTYFAFQNSLGESHKPWLGLNQEKGIVGIGTAKRKIKVDIEAVSGASAAAEGVASTGNGKDHTGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>D5G920</accession>
    <name>D5G920_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00004950001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430053">
        <property type="protein sequence ID" value="CAZ81013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836822.1">
        <property type="nucleotide sequence ID" value="XM_002836776.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G920"/>
    <dbReference type="GeneID" id="9181296"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004950001"/>
    <dbReference type="eggNOG" id="ENOG502SB57">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1526274_0_0_1"/>
    <dbReference type="InParanoid" id="D5G920"/>
    <dbReference type="OMA" id="RWEPESK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019419">
        <property type="entry name" value="AIM19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28177">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 19, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28177:SF1">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 19, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10315">
        <property type="entry name" value="Aim19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ81013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="176" mass="19541" checksum="2371D2A477284DB3" modified="2010-06-15" version="1">MLLVYPLSRVHQLTREIKIHTLSLQSLHQMSGAIAHQAKTFLDTSGRRYAESPLPAWASSIAILYQLPDSKRLYNSRLLPRPWTVLLFSSLFAASGWMAYDNAPIDGAGTTAAWSAIYCIMNGSPRKLGKLLRFGRVAPLGTAGFVAANTITHGYYYLTVPRSKEEVEELNRLTGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9A7</accession>
    <name>D5G9A7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00003228001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S33 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430057">
        <property type="protein sequence ID" value="CAZ81100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836909.1">
        <property type="nucleotide sequence ID" value="XM_002836863.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9A7"/>
    <dbReference type="STRING" id="656061.D5G9A7"/>
    <dbReference type="GeneID" id="9182851"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003228001"/>
    <dbReference type="eggNOG" id="KOG2565">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019414_0_2_1"/>
    <dbReference type="InParanoid" id="D5G9A7"/>
    <dbReference type="OMA" id="RGYQRIQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004301">
        <property type="term" value="F:epoxide hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097176">
        <property type="term" value="P:epoxide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010497">
        <property type="entry name" value="Epoxide_hydro_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016292">
        <property type="entry name" value="Epoxide_hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21661:SF35">
        <property type="entry name" value="EPOXIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21661">
        <property type="entry name" value="EPOXIDE HYDROLASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06441">
        <property type="entry name" value="EHN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001112">
        <property type="entry name" value="Epoxide_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0058">Aromatic hydrocarbons catabolism</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epoxide hydrolase N-terminal" evidence="4">
        <location>
            <begin position="7"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF06441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ81100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="314" mass="35378" checksum="FBD8930C3B10FBD1" modified="2010-06-15" version="1">MPGPTPTPFEIHVPDSLLGFIHDRVRTARLPPPIAHPDGKASTYGVSHETMTALQDHWNNRYNWRAVEARLNSSLKQFTVPILHEGEDLNIHFVHHRSPQPDAIPLIFVHGWPGSFLEVERILTPLTHPPTEKAQAFHVVAPSLPGIGFSSAPKTKNFGPEKIAGVFHKLMMILGYDHYVSQGGDWGSLICRYLAADHPKACLGVHLNFVIALPPLALKNPLELFYLATRWMTPYQKEMIDRMIWWKKEEGGYAEIMSTKPMTIAFALVDSPLGFAAWIRDKIDSLVDEYEWTEEEVITLCMVSILPLVSMGFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9J4</accession>
    <name>D5G9J4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="11">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00003351001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the methylenetetrahydrofolate reductase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430062">
        <property type="protein sequence ID" value="CAZ81187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836996.1">
        <property type="nucleotide sequence ID" value="XM_002836950.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9J4"/>
    <dbReference type="FunCoup" id="D5G9J4">
        <property type="interactions" value="24"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G9J4"/>
    <dbReference type="GeneID" id="9185324"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003351001"/>
    <dbReference type="eggNOG" id="KOG0564">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025841_2_1_1"/>
    <dbReference type="InParanoid" id="D5G9J4"/>
    <dbReference type="OMA" id="GLMPINS"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004489">
        <property type="term" value="F:methylenetetrahydrofolate reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00537">
        <property type="entry name" value="MTHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.220:FF:000002">
        <property type="entry name" value="Methylenetetrahydrofolate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029041">
        <property type="entry name" value="FAD-linked_oxidoreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004621">
        <property type="entry name" value="Fadh2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003171">
        <property type="entry name" value="Mehydrof_redctse-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053806">
        <property type="entry name" value="MTHFR_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00677">
        <property type="entry name" value="fadh2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45754">
        <property type="entry name" value="METHYLENETETRAHYDROFOLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45754:SF1">
        <property type="entry name" value="METHYLENETETRAHYDROFOLATE REDUCTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02219">
        <property type="entry name" value="MTHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21895">
        <property type="entry name" value="MTHFR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51730">
        <property type="entry name" value="FAD-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MTHFR SAM-binding regulatory" evidence="10">
        <location>
            <begin position="358"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="333"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF21895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ81187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="608" mass="67764" checksum="6941BEA1368A7542" modified="2010-06-15" version="1">MDKVVNKIAALPPGEPYHSLEFFPPKTSMGFQNLQARLERMARALRPLFVNVTWGAGGSTATKSLQLAEICQRQLGLTTVLHLTCTNTSQTVLDETLETAKEIGIRNILALRGDPPRSDDYHNTPCDGTSNGAGKEFGYVIDLVRYIREKYGDYFCIGVAAYPEGHVEGASPEKQDPLFDLSFLVEKVKAGADFIMTQLFYDADKYIWFEKLLREEPSGVLSELPVVPGLMPIQSYSMLKRITKLSGASLPAYILRRLDDVKGDDEAVKRVGIDIVSEVIEKIKAANLSGPRGFHFYTLNLEKAVSFIVERTNLIPPLHESVLEEIEGPDMNGAALRRDRRTSSVTSDPHNREGALGREATWDDYPNGRFGDARSPAFGEIDGYGPSLHLSQAQAIKLWSYPVTKQDVSDIFVQHIKGELEAIPWSEQGLSVESKVIADHLAKLNQKGFWTVASQPAINGVNSTDETFGWGPKGGFVFQKAFVEFFCSTEDWEVLKARLERVEDVSYYAANKAGDYVASVPPGGSAMHAVTWGVFPGKEIITPTIIEEVSFKAWKEEAFSIWKEWARVYPPTSASSKLLMETAETYYLVNIIHHGYMDPEFLWKLLET</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9U9</accession>
    <name>D5G9U9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Mitochondrial import inner membrane translocase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">GSTUM_00005081001</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Mitochondrial intermembrane chaperone that participates in the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="function">
        <text evidence="15">Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Heterohexamer; composed of 3 copies of TIM9 and 3 copies of TIM10, named soluble 70 kDa complex. Associates directly with the TIM22 complex, whose core is composed of TIM22 and TIM54. Interacts with the transmembrane regions of multi-pass transmembrane proteins in transit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Mitochondrion inner membrane</location>
            <topology evidence="1 15">Peripheral membrane protein</topology>
            <orientation evidence="1 15">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="15">The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the small Tim family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430064">
        <property type="protein sequence ID" value="CAZ81292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837101.1">
        <property type="nucleotide sequence ID" value="XM_002837055.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9U9"/>
    <dbReference type="FunCoup" id="D5G9U9">
        <property type="interactions" value="605"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G9U9"/>
    <dbReference type="GeneID" id="9188535"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005081001"/>
    <dbReference type="eggNOG" id="KOG3480">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_162151_1_0_1"/>
    <dbReference type="InParanoid" id="D5G9U9"/>
    <dbReference type="OMA" id="VGENMQK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042719">
        <property type="term" value="C:mitochondrial intermembrane space chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042721">
        <property type="term" value="C:TIM22 mitochondrial import inner membrane insertion complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140318">
        <property type="term" value="F:protein transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.810:FF:000002">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit tim10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.810">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit tim13 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004217">
        <property type="entry name" value="Tim10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035427">
        <property type="entry name" value="Tim10-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11038">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11038:SF16">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02953">
        <property type="entry name" value="zf-Tim10_DDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144122">
        <property type="entry name" value="Tim10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 15" id="KW-0143">Chaperone</keyword>
    <keyword evidence="11 15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 15" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7 15" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 15" id="KW-0811">Translocation</keyword>
    <keyword evidence="3 15" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Tim10-like" evidence="16">
        <location>
            <begin position="17"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00055537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00065568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU367043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAZ81292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="96" mass="10497" checksum="7B5B103609E8924E" modified="2010-06-15" version="1">MSFLGFGKPQPTLAEKMTAAEQELEMITDMFNRLTRSCHNKCIPTDYKEADLNKGESVCLDRCVSKFFDVNIKVSEAMQSQSQAMRGAQGGGGLGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9Y5</accession>
    <name>D5G9Y5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00003450001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430066">
        <property type="protein sequence ID" value="CAZ81328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837137.1">
        <property type="nucleotide sequence ID" value="XM_002837091.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9Y5"/>
    <dbReference type="GeneID" id="9188571"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003450001"/>
    <dbReference type="HOGENOM" id="CLU_2962572_0_0_1"/>
    <dbReference type="InParanoid" id="D5G9Y5"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ81328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="59" mass="6662" checksum="A8B4B8BEB6B56E70" modified="2010-06-15" version="1">MAIFAFFFTIVSYQYSSTKCEKGPVRVSFIRSLGLGVVDLAFFYFLLCEPYLGCCVIIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GA51</accession>
    <name>D5GA51_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00005134001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430075">
        <property type="protein sequence ID" value="CAZ81405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837214.1">
        <property type="nucleotide sequence ID" value="XM_002837168.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GA51"/>
    <dbReference type="STRING" id="656061.D5GA51"/>
    <dbReference type="GeneID" id="9187103"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005134001"/>
    <dbReference type="eggNOG" id="KOG1205">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_48_0_1"/>
    <dbReference type="InParanoid" id="D5GA51"/>
    <dbReference type="OMA" id="IDVIANC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051911">
        <property type="entry name" value="SDR_oxidoreductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43976">
        <property type="entry name" value="SHORT CHAIN DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43976:SF16">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE_REDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ81405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="364" mass="40330" checksum="07F585D3DA5BAC13" modified="2010-06-15" version="1">MDTYQTAHNPESSTQSSNIYPRTYLPASSEPPSPSKPSEQAVWLIFGATGHLGRSICRVALARGDCVTAVGRNLPGEEAVMQGWHQMCQGLVCDVRVRDTVSKVFASAMDRWGKVDIVVNCTGYGIIGACEDQDEHEIRAQFETNLIGAANIFQLTLPYFRGRGSGMYIVFSCTAGTLGIPGLGPYCATKWAVEGLTESLMYEVEPLGIKVTIVVPGLSRRDELGPRLIPLLTKQAGREPQATNSPAQHATRMIDWLKYKEPTSAIRIAEITWELGHSAHPPLRLFLGDHAVEAVRDRLRSTIEEVRSYLLLYLNVCLGGWELFLVRTGTQGMLTRVWQIEDWKHLNFRSEDEEGIVVARAGNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GA65</accession>
    <name>D5GA65_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00005153001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. AB hydrolase 4 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430075">
        <property type="protein sequence ID" value="CAZ81419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837228.1">
        <property type="nucleotide sequence ID" value="XM_002837182.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GA65"/>
    <dbReference type="FunCoup" id="D5GA65">
        <property type="interactions" value="239"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GA65"/>
    <dbReference type="ESTHER" id="tubmm-d5ga65">
        <property type="family name" value="abh_upf0017"/>
    </dbReference>
    <dbReference type="GeneID" id="9185701"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005153001"/>
    <dbReference type="eggNOG" id="KOG1838">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032487_1_0_1"/>
    <dbReference type="InParanoid" id="D5GA65"/>
    <dbReference type="OMA" id="MMTTSWG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008126">
        <property type="term" value="F:acetylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051792">
        <property type="term" value="P:medium-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051793">
        <property type="term" value="P:medium-chain fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050960">
        <property type="entry name" value="AB_hydrolase_4_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012020">
        <property type="entry name" value="ABHD4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794">
        <property type="entry name" value="ABHYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794:SF63">
        <property type="entry name" value="ALPHA_BETA HYDROLASE 1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005211">
        <property type="entry name" value="Ab_hydro_YheT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="4">
        <location>
            <begin position="126"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="2">
        <location>
            <position position="212"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="2">
        <location>
            <position position="341"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="2">
        <location>
            <position position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR005211-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ81419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="382" mass="42209" checksum="AD5AB40C4EC94B73" modified="2010-06-15" version="1">MPFWPAHPTSHHSNNPITLKTKTKTLPFPALTTPLLTPFHANPFLFNGHLQTFWTAAKWDDPHAIYYARQLLRNPADGGHFAVDFVVPEASPSTPAPAATGGNLPSRTRDMTEEEVAKLGSDDDTPMVIALHGLSGGSHELYLRSVLAPLTRKVEEGGSGFAACVVNARGCALSKVTTGQLFNARFTEDLRQTVRYLQDVYPRRPLYAVGFSFGANILANYIGEEGGKCAFKAVALCSCPWNLEVASKALQRTYLGSEVYSKVMGGNLRNLFEIHIDNFSGDPRIDVDLVRRGRYLYEFDRDFTARIFGYPTVGAYYRDASSVDNLLKARVPTFILHAKDDPISVDEGVPYDEVKANPYCFMATTPTGGHLSWFEYGGGRWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAD5</accession>
    <name>D5GAD5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">E3 ubiquitin protein ligase</fullName>
            <ecNumber evidence="15">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">GSTUM_00005251001</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H2B to form H2BK123ub1. H2BK123ub1 gives a specific tag for epigenetic transcriptional activation and is also a prerequisite for H3K4me and H3K79me formation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 15">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the BRE1 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="15"/>
    <dbReference type="EMBL" id="FN430075">
        <property type="protein sequence ID" value="CAZ81489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837298.1">
        <property type="nucleotide sequence ID" value="XM_002837252.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAD5"/>
    <dbReference type="FunCoup" id="D5GAD5">
        <property type="interactions" value="866"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GAD5"/>
    <dbReference type="GeneID" id="9182524"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005251001"/>
    <dbReference type="eggNOG" id="KOG0978">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019713_2_0_1"/>
    <dbReference type="InParanoid" id="D5GAD5"/>
    <dbReference type="OMA" id="YRQMQEY"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033503">
        <property type="term" value="C:HULC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16499">
        <property type="entry name" value="RING-HC_Bre1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058643">
        <property type="entry name" value="BRE1-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013956">
        <property type="entry name" value="E3_ubiquit_lig_Bre1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23163:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE BRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23163">
        <property type="entry name" value="RING FINGER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08647">
        <property type="entry name" value="BRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26095">
        <property type="entry name" value="CC_Bre1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 15" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="11 15 16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 15" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9 15" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 14 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="18">
        <location>
            <begin position="670"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="233"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="181"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="260"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="330"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="429"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="23"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="233"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU365038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAZ81489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="722" mass="82762" checksum="505DEA5B53458ADF" modified="2010-06-15" version="1">MEDRKRPVPHEPSDDSLPPFKRPALNSASSSQAPQNSHQVPQSQEDVIHFQKEAIWRQMMEYKRERNMLESRVEELDKRSTYHDDHLRVIDAWWAQLLDEVRFLAGEPDSSCQNGASLEFSSSLLFNDIASFTDHLKEKRDHITSSLQALFSAIRSNSSNNKKADGVEELQSRLSSLLANEKARKVEIARLQKEKEDANSRLTEATIKYMTAEKKVDRLKSQTLAKIERQAMFQSNAGAENNKDGGNSDEQSAKDREINAKVAEGNVADAEQARKEAQAIASKQKEELQQLQADNTRLSEQVTTFTIKFGRLSEEDISKCDAYKNLRVKLEDLATRFNHIEALNRELLERTEHFEAERTKYKELILTEQHTVISDMQLQLSRTEQDLARVRAARDELIQDQTQRKTREDQKLGSIREVNELAETRASRIASLEMEVERLRLELDKEALSDTQPFAEWGLDELRQKYEHLDKAYKVLTQELPALEQAFKKAHDQASRKVAGIQESEDKMRRLQAEKGKADQKYFSAMKNKETLSAENRALKNLNARSTDIITQLKDAEKKVRDLVVNLEKQIAEMKTVQQTLITRNREHQSRVAEQNNTTDSLKSQVAELSTYLKTRDAAVSRESAGRREAEIEVEKLQVKLEDAQRCLENNRPKGSENSQLEALRQIALCTVCRNRFKNTAIRPCGHVFCRQCADERISSRSRKCPNCGRAFAVTDLISVHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAI3</accession>
    <name>D5GAI3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00003602001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I 49 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430078">
        <property type="protein sequence ID" value="CAZ81526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837335.1">
        <property type="nucleotide sequence ID" value="XM_002837289.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAI3"/>
    <dbReference type="STRING" id="656061.D5GAI3"/>
    <dbReference type="GeneID" id="9187466"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003602001"/>
    <dbReference type="eggNOG" id="KOG2870">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1788221_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAI3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006120">
        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.645.10">
        <property type="entry name" value="Cytochrome-c3 Hydrogenase, chain B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014029">
        <property type="entry name" value="NADH_UbQ_OxRdtase_49kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022885">
        <property type="entry name" value="NDH1_su_D/H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11993:SF10">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11993">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 49 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="HydB/Nqo4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00535">
        <property type="entry name" value="COMPLEX1_49K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-1278">Translocase</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ81526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="145" mass="16437" checksum="2260180D13E1E547" modified="2010-06-15" version="1">MASTFARLAARSLTCARPLAVSSPIRAARAIRPFKSTQISSAEAAPYQRTTLTELEPKNETTDDYLTEDPNHDPVHGRKMRHYTVNFGPQHPAAHGVLRLILELNGEEIIRADPHVGLLHRGTEKLIEYKTYLQALPYFDRLDYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>D5GB50</accession>
    <name>D5GB50_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="14">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00005449001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ubiquitin family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430086">
        <property type="protein sequence ID" value="CAZ81743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837552.1">
        <property type="nucleotide sequence ID" value="XM_002837506.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GB50"/>
    <dbReference type="STRING" id="656061.D5GB50"/>
    <dbReference type="GeneID" id="9181454"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005449001"/>
    <dbReference type="eggNOG" id="KOG0001">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010412_4_1_1"/>
    <dbReference type="InParanoid" id="D5GB50"/>
    <dbReference type="OMA" id="GWHANIC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01803">
        <property type="entry name" value="Ubl_ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000004">
        <property type="entry name" value="Polyubiquitin Ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000427">
        <property type="entry name" value="Putative Polyubiquitin-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1110.10">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035896">
        <property type="entry name" value="AN1-like_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019954">
        <property type="entry name" value="Ubiquitin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019956">
        <property type="entry name" value="Ubiquitin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050158">
        <property type="entry name" value="Ubiquitin_ubiquitin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000058">
        <property type="entry name" value="Znf_AN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10666">
        <property type="entry name" value="UBIQUITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01428">
        <property type="entry name" value="zf-AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00348">
        <property type="entry name" value="UBIQUITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00154">
        <property type="entry name" value="ZnF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118310">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00299">
        <property type="entry name" value="UBIQUITIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51039">
        <property type="entry name" value="ZF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="12">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="12">
        <location>
            <begin position="89"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="AN1-type" evidence="13">
        <location>
            <begin position="136"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ81743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="205" mass="23164" checksum="63876000C7B4B4CE" modified="2010-06-15" version="1">MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGAPKKKICNFKECTTPAQRIVGDCRFCDGHYCGKHRLLEDHKCSGLEDCRKESHDRNAIKLNSERTVVGKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBA4</accession>
    <name>D5GBA4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00000538001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430089">
        <property type="protein sequence ID" value="CAZ81797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837606.1">
        <property type="nucleotide sequence ID" value="XM_002837560.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBA4"/>
    <dbReference type="GeneID" id="9181508"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000538001"/>
    <dbReference type="HOGENOM" id="CLU_1046589_0_0_1"/>
    <dbReference type="InParanoid" id="D5GBA4"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="266" mass="29201" checksum="F06D6B3ED34AEA65" modified="2010-06-15" version="1">MVLMILRVPVFSVNPQTTPPGERDTVSPILGGLSKKKGKKKSQSLRARTESDSLGCWNRAGLGGTPDAFEHSFSDEYSLRFALALPGYYATAIGILLGGAQDRTNTLPGGVRGSYCTVQVRSWSATHTIHELPKILTSIQGKKERALVSLRLHLLLGPSSSCRGTLKLGFSGVCAPPPILSHSSCRGLKKPSCYGGPILDTCSILWNVMLVGNTRVQYSAVLRNLWHKRLTISRYSAGTVLRMRDCLKILREAQYRTVLYRGSFFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBG2</accession>
    <name>D5GBG2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00000459001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Non-catalytic component of the proteasome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase T1B family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430094">
        <property type="protein sequence ID" value="CAZ81855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837664.1">
        <property type="nucleotide sequence ID" value="XM_002837618.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBG2"/>
    <dbReference type="FunCoup" id="D5GBG2">
        <property type="interactions" value="1154"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GBG2"/>
    <dbReference type="MEROPS" id="T01.A13"/>
    <dbReference type="GeneID" id="9184429"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000459001"/>
    <dbReference type="eggNOG" id="KOG0185">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072435_0_1_1"/>
    <dbReference type="InParanoid" id="D5GBG2"/>
    <dbReference type="OMA" id="QPIMRRY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034515">
        <property type="term" value="C:proteasome storage granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03760">
        <property type="entry name" value="proteasome_beta_type_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000014">
        <property type="entry name" value="Proteasome subunit beta type-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016295">
        <property type="entry name" value="Proteasome_beta4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF6">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001213">
        <property type="entry name" value="Psome_endopept_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 4" id="KW-0647">Proteasome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR001213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ81855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="260" mass="28700" checksum="B716B96F8ED1C66C" modified="2010-06-15" version="1">MNHLPEAWGRPRNDVYGTYDASYLQSSMPNQHTQSPIVTGTSVVALKFNGGVVIAADNLASYGSLARFTNVERLKQVGTHTVVGAGGDISDMQYLHEKLLDSLIIKEEYQNDGHHLRASHIYRYLSKVLYQRRCKFDPLWNTLLVAGWDDGKPFLASADLLGTTFSAPALATGFGAHLAVPLLRRVCPDEAAVENITREQAVDTIEECMKVLFYRDARSLSKYSLAIITGKDVEGAAECEAKNQSWKFAEGIRGYGTQVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBN5</accession>
    <name>D5GBN5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00005493001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ81885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837694.1">
        <property type="nucleotide sequence ID" value="XM_002837648.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBN5"/>
    <dbReference type="STRING" id="656061.D5GBN5"/>
    <dbReference type="GeneID" id="9182338"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005493001"/>
    <dbReference type="eggNOG" id="ENOG502QU5B">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010147_0_0_1"/>
    <dbReference type="InParanoid" id="D5GBN5"/>
    <dbReference type="OMA" id="QHRYHSR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070772">
        <property type="term" value="C:PAS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010513">
        <property type="term" value="P:positive regulation of phosphatidylinositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903778">
        <property type="term" value="P:protein localization to vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000011">
        <property type="term" value="P:vacuole inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024260">
        <property type="entry name" value="Vac7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28258">
        <property type="entry name" value="VACUOLAR SEGREGATION PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28258:SF1">
        <property type="entry name" value="VACUOLAR SEGREGATION PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12751">
        <property type="entry name" value="Vac7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="647"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="249"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="352"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="729"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="211"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="302"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="426"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="479"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="501"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="536"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="578"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="729"/>
            <end position="743"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ81885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="888" mass="93295" checksum="4B8FE4406282EA3A" modified="2010-06-15" version="1">MSAARAAVNNRGSGSVAGTGNGVPAAGNGGVDLGGGEGGGSGAELDGHSHDRINSELADAEKHSRDSSPMRRNTSSAGLSDSNTSTSTLKKNRKPNAVRSATPSRETSPTRSGALAAGSSRNAQLQSQPDLHQPRPHRPLANAPHWPPSTRLAVTRTPPPDSRSPALSPQKKGDRTNYSTLTGVPQSVHLLREGNSEDLEESGLASGMRTPRSNNSPALETVVESSLPTTPAINTSKTLTEQVAALINRQQHNTIPEEPEGEKSGARGTRKYRTGSIASSIAPRSQAESESESGYKSEEKAAYANSNNNSRAPPPLKGFARRPAVMGGDNFSRNMTVETETVSSVPQVAVGAAGVPGGASIRSKKSSDTIRAPKKEKKKPPKRTGGGGPANPSSKADLFARRVAEAVDEANSSDSEETFVYESNPPEAQQRPTSRFHSRTPSATSIQGGPDPQGLRLPLLSSMDGNQSTRRGRMKFASASVSGNTNVASDGTTGEASGDRAGTGGSGPPTAIGGAGGSRSEGQSGSGQKEKGGSSRGQNGNRGLLLEESPFSSSHKSSTHPPRPGSTLPPRQSTSGRHGSGLRNGGNGYGSKKLPRGWASYEADGEGAGDERVPLINRPGRNHRHGRRPGSGTLRQLEHSQLQRRGWFRTYICCIIAVFAVLLIMTGVGGFLFATTKALQEVKVLEITGVLVSKQELMLDLVVSAVNPNAIPVVVGNMDVNLFAKSAHVGDGKGKDEDGRDGDGDGDGSAGDNDPEGDPWWGLLSKRRKKQARRTDPPEDLPEQDRQTMLLGRIFSFDSALSFDGSPLRHRHSISVGELRLAKPGNKTEEGGTERWERVLQHPFELIVRGVLKYQLPLSGRVRTAPIGASVLVHPDGAASAVSPETVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBQ2</accession>
    <name>D5GBQ2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glutamate-5-semialdehyde dehydrogenase</fullName>
            <ecNumber evidence="2">1.2.1.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Glutamate-5-semialdehyde dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Glutamyl-gamma-semialdehyde dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00005520001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-glutamate 5-semialdehyde + phosphate + NADP(+) = L-glutamyl 5-phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19541"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58066"/>
            <dbReference type="ChEBI" id="CHEBI:58274"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.2.1.41"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the gamma-glutamyl phosphate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.41" evidence="2"/>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ81902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837711.1">
        <property type="nucleotide sequence ID" value="XM_002837665.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBQ2"/>
    <dbReference type="FunCoup" id="D5GBQ2">
        <property type="interactions" value="850"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GBQ2"/>
    <dbReference type="GeneID" id="9182939"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005520001"/>
    <dbReference type="eggNOG" id="KOG4165">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030231_0_1_1"/>
    <dbReference type="InParanoid" id="D5GBQ2"/>
    <dbReference type="OMA" id="KTQRYGT"/>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00360"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004350">
        <property type="term" value="F:glutamate-5-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd07079">
        <property type="entry name" value="ALDH_F18-19_ProA-GPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000006">
        <property type="entry name" value="Gamma-glutamyl phosphate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00412">
        <property type="entry name" value="ProA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020593">
        <property type="entry name" value="G-glutamylP_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012134">
        <property type="entry name" value="Glu-5-SA_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000965">
        <property type="entry name" value="GPR_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001221">
        <property type="entry name" value="PRK00197.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00407">
        <property type="entry name" value="proA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11063:SF8">
        <property type="entry name" value="DELTA-1-PYRROLINE-5-CARBOXYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11063">
        <property type="entry name" value="GLUTAMATE SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000151">
        <property type="entry name" value="GPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01223">
        <property type="entry name" value="PROA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0641">Proline biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="12">
        <location>
            <begin position="15"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00059423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00060997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00075718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00077451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ81902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="448" mass="47775" checksum="5B5BED435F075E83" modified="2010-06-15" version="1">MATTAAAPAEIAHTSSLASRVLKVVSGSDRSAALTAVYEHLQAAKDEILEANKRDLEAAEEQAKAGKLSLSLIKRLDLCRSGKWEDMVQGILDVRELEDPVGKITLATKLDQNLDLYRLTSPIGVLLVIFEARPEVIANITALALKSGNAVILKGGKESTHSFAAIAGVISTALSTTSIPPGAVQLVLSREDVNALLDQDQYIDLVIPRGGNELVRYIKDHTKIPVMGHADGLCTIYVHADADETKAVKVVLDAKTDYPAACNAVETLLVHQSICSTILPKIAAMLLAAGVSLRCDTSSRAVLEASSLPPSSKAIIQDATAADYDTEFLELTLAVKVVLSVEEAVGHINAHGSGHTDTIISEDEEIAEKFMAGVDSAGVFWNASTRFADGFRYGFGTEVGISTNKIHARGPVGLEGLMIYKYQLRGNGQGAGEYGSGRKPFLHQKIEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBS5</accession>
    <name>D5GBS5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00005557001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ81925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837734.1">
        <property type="nucleotide sequence ID" value="XM_002837688.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBS5"/>
    <dbReference type="STRING" id="656061.D5GBS5"/>
    <dbReference type="GeneID" id="9182962"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005557001"/>
    <dbReference type="eggNOG" id="KOG4120">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042829_1_0_1"/>
    <dbReference type="InParanoid" id="D5GBS5"/>
    <dbReference type="OMA" id="ETENMGR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008263">
        <property type="term" value="F:pyrimidine-specific mismatch base pair DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004844">
        <property type="term" value="F:uracil DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006285">
        <property type="term" value="P:base-excision repair, AP site formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10028">
        <property type="entry name" value="UDG-F2_TDG_MUG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.470.10:FF:000010">
        <property type="entry name" value="G/U mismatch-specific DNA glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.470.10">
        <property type="entry name" value="Uracil-DNA glycosylase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015637">
        <property type="entry name" value="MUG/TDG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005122">
        <property type="entry name" value="Uracil-DNA_glycosylase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12159">
        <property type="entry name" value="G/T AND G/U MISMATCH-SPECIFIC DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12159:SF9">
        <property type="entry name" value="G_T MISMATCH-SPECIFIC THYMINE DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03167">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="Uracil-DNA glycosylase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0234">DNA repair</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Uracil-DNA glycosylase-like" evidence="5">
        <location>
            <begin position="157"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="84"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="21"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ81925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="357" mass="39554" checksum="FE81E8EB8484774E" modified="2010-06-15" version="1">MSNLSTSSIGQGEGRNGQAEMSKRKYADKVKDEEVVEESEEEEVEDEDLDEEEELYPSFGGLLSGFAFASSSYSRAPIGALAIASRPTLPGNPPPTSRNPRPKKPSPVRMESRLPAGPSPPPTRPKKKRRTNTGYVPPSAYAHLPLLPDTLEENLICAFTGINPGLTTARIGHSFANPTNLFWPLMHSSGCTPDRRLPPQMDGRLPDLYSLGLANLVNRPTKGVAELSKEEMLAGVAVLEEKVRRYKPEAVCFVGKVAWEAVFKVRNGRGIRKGEFDWGWQEGVRFGVEVGEGRWRGCRVFVVPSTSGLVASYGPEFKRALWKRLGDWVQMRRGERGENAPRVLEWEENGEGETVIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>D5GC42</accession>
    <name>D5GC42_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00000554001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <dbReference type="EMBL" id="FN430100">
        <property type="protein sequence ID" value="CAZ82085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837894.1">
        <property type="nucleotide sequence ID" value="XM_002837848.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GC42"/>
    <dbReference type="STRING" id="656061.D5GC42"/>
    <dbReference type="GeneID" id="9181354"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000554001"/>
    <dbReference type="eggNOG" id="KOG4642">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056455_1_1_1"/>
    <dbReference type="InParanoid" id="D5GC42"/>
    <dbReference type="OMA" id="WAGVEHD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071218">
        <property type="term" value="P:cellular response to misfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045862">
        <property type="term" value="P:positive regulation of proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46803">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE CHIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46803:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE CHIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0697">Rotamase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="U-box" evidence="6">
        <location>
            <begin position="180"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ82085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="256" mass="29070" checksum="DDCAA51A7F2D4C86" modified="2010-06-15" version="1">MPMKAEELKNKGNVCYAEGDYVGAEKFYTQAIIKDSTNAAFFTNRALVRLRMDMYDQVIDDCLKAIDLIPSSLKAYSYLGQAQLKLGYPNEALSSTLRAYELAIAQRSPSVGAIAATCLEVKKKRWELSEERRRTRECNLAMRNARLILFFVPLPGTLEKIQTLEEVFGKAEAERCGKREVPDYLIDNITFSVMLDPVITKYGHSYDRVTLLDHLKRSSTDPLTREPLTEKDLRPNLALKAACEAFLKENGWAVDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCM4</accession>
    <name>D5GCM4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00000728001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430114">
        <property type="protein sequence ID" value="CAZ82267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838076.1">
        <property type="nucleotide sequence ID" value="XM_002838030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCM4"/>
    <dbReference type="STRING" id="656061.D5GCM4"/>
    <dbReference type="GeneID" id="9187886"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000728001"/>
    <dbReference type="eggNOG" id="KOG3872">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021864_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCM4"/>
    <dbReference type="OMA" id="VEHAGFM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22679">
        <property type="entry name" value="FHA_SLMAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051176">
        <property type="entry name" value="Cent_Immune-Sig_Mod"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15715">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 170 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15715:SF46">
        <property type="entry name" value="TO VACUOLE TARGETING VPS64, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G02420)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="720"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="domain" description="FHA" evidence="4">
        <location>
            <begin position="169"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="39"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="300"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="387"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="467"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="579"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="43"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="300"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="310"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="395"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="434"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="446"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ82267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="748" mass="81732" checksum="362EFD1A401B96F2" modified="2010-06-15" version="1">MTAVAQAPNFLSVPGSWNGSGPSKLNSVTHDDVARIFIKGQRSSSMSSVSSTSGTSNGSEAGWGTSVNGKKKGVSRAATRGANATWATKSDMMRVPGSSNQPQGGLLAGSNSSINGNHQAMPILPSQHLISGQTQQNGGPLPPSYLMLLPLNGTFDRKFIPLPYWPDTLRIGRQTNNKTTPTQNNGYFDSKVLSRQHAEIWAEKLNGRVWIRDIKSSNGTFVNGQRLSQENRDSEPHELRAEDVLELGIDIVGEDNKTIIHHKVAARVEHAGLQTPNVGNFDLNFVDIDPMNSVSIMNPQNNSAPLQNTSIRGRSGSQGSRSSVMSGGPASHRQPQMMITPVTMEMVVKKLNYELQSAKQQSQDLQRTSEVFDSLLSSRPIVALGHIAPSANPAPTEPLPAPPVPTHTGLAQRQVSPPSLIPLSTHIQEHPRGQQLSDSSRNTPKLRSEKTERPEPPQHLHGLVGALEEARGELQAKTIRVRELEETLKRERSAREVAEVRAAQLENASRGVRETARRRGFKEFDAEKIRADEAIGLSDEVHELRNEKSLDCHPGALDEEDRDIYDDDDAASVTVDPSAQAAAEAAERLQRRVEQMMAELQSAKEEIERYKRQAEASEHESIKSKKTLGEMVEKIRKDEQARKLLHEREVASQTDSVAVKNTGIQVSSEGIEEAAEDFHLNGTIKPTGGKTAALNESRNSGEMINKRSKECSLAISKRDAAPYASMIGVMLIGVGLMAVLNRWHKTEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCQ6</accession>
    <name>D5GCQ6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00005968001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430120">
        <property type="protein sequence ID" value="CAZ82299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838108.1">
        <property type="nucleotide sequence ID" value="XM_002838062.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCQ6"/>
    <dbReference type="STRING" id="656061.D5GCQ6"/>
    <dbReference type="GeneID" id="9187918"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005968001"/>
    <dbReference type="eggNOG" id="ENOG502SD64">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_479134_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCQ6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="400"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="432"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="528"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="572"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="620"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="677"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="348"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="620"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="636"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="677"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="688"/>
            <end position="702"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ82299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="702" mass="80643" checksum="A8498C1E1AA191D0" modified="2010-06-15" version="1">MEGFLALGDCFGNWSFWETSKGKEKFLKSMADMPNYDWGGERNALFKPSRTGKLDRFRGGKALLKLQEKRDRRTDKKRILVMGDCMIQHLISLMANLDLQEAGHIGEFLSRLRHTDSHKMRFEEKTLMPDNLWITEFNINFIITAPFQADVESPLLPNYFPRRRAKFLSMNDVEPDHIMAEAAMGFRIIGDLHDRYWTCYVFYDFGSREPVTDAWSRVDHLDSCKTPSQRKCLEGFLVRHALDLVLSEAKIILGIIAKPMGGNKKSQSLFNPLLTEDDLSGENYFKTMNKNSVFYPWLLEVCSALLEKCKETRSVAEQWISAEKTREYKPRWSEKDQKSFGEEVAKNRADVMARCAKLEKMAKNLQERIDRNKTLKESLSSELALREARTSTQLAHTVNLFAVVTTIYLPLTFSTSIVAIQDLHWKYPAKTLVRIMLAVTFGTLILLMNLAFLRRHFTTLKTWAQDSIRQRMEGASEPKGSASKEHIYQEKQPAWKYWKERARGLQEAEKRSTLLTDNVSHDGESNWWYWYFMAIYIAVVVPVQELTFIILTLRFQKIKDAGPLKKLVRLPWAPIWILQLALVYVVILAGHTALSLSRLAHRTAVWLWTGNDVAESWKAAQEEEDRKSSVPEPEADDKSLKPEPDSTKKGGVLAAWFKKPAKIMQLLIVAEALPSKEKEIDEEKQETAVEATAKTAEVPSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCT7</accession>
    <name>D5GCT7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="16">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">GSTUM_00000822001</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430121">
        <property type="protein sequence ID" value="CAZ82330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838139.1">
        <property type="nucleotide sequence ID" value="XM_002838093.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5GCT7">
        <property type="interactions" value="275"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GCT7"/>
    <dbReference type="GeneID" id="9187948"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000822001"/>
    <dbReference type="eggNOG" id="KOG0054">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_27_3_1"/>
    <dbReference type="InParanoid" id="D5GCT7"/>
    <dbReference type="OMA" id="CFETGMR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042592">
        <property type="term" value="P:homeostatic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18595">
        <property type="entry name" value="ABC_6TM_MRP1_2_3_6_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18603">
        <property type="entry name" value="ABC_6TM_MRP1_2_3_6_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000020">
        <property type="entry name" value="ABC metal ion transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000450">
        <property type="entry name" value="ABC transporter C family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000001">
        <property type="entry name" value="ATP-binding cassette subfamily C member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003838">
        <property type="entry name" value="ATP-dependent bile acid permease, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056227">
        <property type="entry name" value="TMD0_ABC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF443">
        <property type="entry name" value="MULTIDRUG-RESISTANCE LIKE PROTEIN 1, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24357">
        <property type="entry name" value="TMD0_ABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-1278">Translocase</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="5" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="62"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="129"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="160"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="422"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="502"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="545"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="851"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="895"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="982"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="15">
        <location>
            <begin position="283"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="14">
        <location>
            <begin position="598"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="15">
        <location>
            <begin position="855"/>
            <end position="1139"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="14">
        <location>
            <begin position="1176"/>
            <end position="1386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAZ82330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1402" mass="155307" checksum="8F0501A63698F7ED" modified="2010-06-15" version="1">MRGNFCGDPEGWGPISPHGRSDFTPCFLDSVIDGVSLGIIFFGAFQVYWLSRKKPVGAVKDWSYTLKLGLVAGIIAVSALLASLQVEYSDSWFYDIRFWSPILSCVGLGVAFTVHYLEHDRSWVPNGVLLFYWLFFIVIHGVELRSSVDLHDHKHRLPRLIAVTCAEAAGIFVFLLQLIVPRKRGPYALVGEDEVENECPSEIANVFSRLTFGWMTVMMKRGYNKFLTEEDLWNLRKIDTTRATGDQFTEAWEKELQKEKPSIWIALLRAFGAPYARGSLYKIGNDVLAFVQPQLLRLLIAFVSSHNSNNPQPVVRGVAIAFAMFGSSVVQTLCLHQYFQHAFETGMRIKSSLTAAIYKKSMRLSNEGRASKTTGDIVNLQAVDTQRLQDITQYGQQLWSAPFQIALCMVSLYQLLGPSMFAGIAAMVVMIPVNGIIARVMKTLQKAQMKNKDSRTRLMTEILNNMKSIKLYGWGSPFMAKLDHVRNDLELHTLRKIGVAQAFASFTWSSTPFLVSCSTFAVFVWTQDKPLTTDIVFPALTLFNLLTFPLSVLPMVISAIVEASVAVGRITSFLMAEELQLDAVIHEPPVLEMGEEALNITNATFAWNRAEAGKSALKNINFTAKKGELNCIVGRVGAGKSSFLQAILGDLWKVSGKVVVRGSTAYVAQSAWVMNASVRENIVFGHRYDPEFYQATVRACALLEDFDALPDGDETEVGEKGISLSGGQKARLTLARAVYARADVYLLDDPLSAVDQHVGRHLIDNVLGPKGLLAGKTRILATNSIPVLMEADYIHLIVAGEIAETGTYNTSDKDSDRTIGPEMRKSRRKEFSEKGKVKWSVYGEYAKASNLFAVAIYLLALIFSQVVSVGGNVWLKTWSEANGENHANDHVGKYLGIYFAFGVGAAALVVFQTLILWIFCAIEAARKLHNKMAVAIFRAPMQFFETTPAGRILNRFSSDIYRIDEVLCRSFNMLFSNSARSIATIAIISISTPPFLALVVPLGCLYLYIQRYYLRTSRELKRLDSTSRSPIYAHFQESLGGISTIRAYQQQSRFIAENEWRIDANLRAYFPSINANRWLAVRLEIIGSVVILGAAGFPIIAVASGTNLSAGMVGLSMSYALQITQSLNWIVRQTVEVETNIVSVERVLEYAALPSEAPEVIPSQRPSTSWPANGAVSFHNYSTRYRPGLDLVLKDINIDIKPREKIGIVGRTGAGKSSLTLALFRIIEPANGNISVDSLNTSAIGLRDLRQRLAIIPQDASLFEGTVRDNLDPSHVHDDTELWTVLELSNLKAKVSSMDGKLDAQVHEGGSNLSVGERALMQTDNMLQRTIRDRFRDRTILTIAHRLNTIIDSDRVIVLQAGRVAEFDTPEALLSDKNSLFFALAKEAGLTQAGSSSELPVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDD0</accession>
    <name>D5GDD0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00006156001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family. Azg-like subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430142">
        <property type="protein sequence ID" value="CAZ82523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838332.1">
        <property type="nucleotide sequence ID" value="XM_002838286.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDD0"/>
    <dbReference type="STRING" id="656061.D5GDD0"/>
    <dbReference type="GeneID" id="9182548"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006156001"/>
    <dbReference type="eggNOG" id="ENOG502QQ5E">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_970408_0_0_1"/>
    <dbReference type="InParanoid" id="D5GDD0"/>
    <dbReference type="OMA" id="NEEYAIC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005345">
        <property type="term" value="F:purine nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015853">
        <property type="term" value="P:adenine transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015854">
        <property type="term" value="P:guanine transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045018">
        <property type="entry name" value="Azg-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006043">
        <property type="entry name" value="NCS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43337">
        <property type="entry name" value="XANTHINE/URACIL PERMEASE C887.17-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43337:SF1">
        <property type="entry name" value="XANTHINE_URACIL PERMEASE C887.17-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00860">
        <property type="entry name" value="Xan_ur_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="69"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="161"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="213"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ82523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="287" mass="31170" checksum="A9ACD8BBD7434E03" modified="2010-06-15" version="1">MTWIDRTNRRVADSSFGRYFRLEHSGHRKERKGSRFFTEIRAGLATLISLGQKHSEEYMLCVGIVQRDLVTATAAIAALTSFFMGLGANIPIACAPGMGLNAYFAYQVVGYHGSGPVPYRLAVTAVFVEGFIFVALSILGMRQWLARAIPASIKLATGAGIGLYLALIGLTYSAGIGAITGAVSTPLDLAGCKPEFRDEVTGVCEGYKLRNPTLWIGIFCGGIVTAFLMMYRVKGAIIAGIALVSIISWPRPTEVTYFPYNSEGNQSDVQQMEHVSPTNLWENSQHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDG0</accession>
    <name>D5GDG0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">serine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Seryl-tRNA synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Seryl-tRNA(Ser) synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00001005001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.1.1.11" evidence="1"/>
    <dbReference type="EMBL" id="FN430143">
        <property type="protein sequence ID" value="CAZ82561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838370.1">
        <property type="nucleotide sequence ID" value="XM_002838324.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDG0"/>
    <dbReference type="FunCoup" id="D5GDG0">
        <property type="interactions" value="806"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GDG0"/>
    <dbReference type="GeneID" id="9186883"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001005001"/>
    <dbReference type="eggNOG" id="KOG2509">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023797_4_3_1"/>
    <dbReference type="InParanoid" id="D5GDG0"/>
    <dbReference type="OMA" id="EQNCIDR"/>
    <dbReference type="UniPathway" id="UPA00906">
        <property type="reaction ID" value="UER00895"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004828">
        <property type="term" value="F:serine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070158">
        <property type="term" value="P:mitochondrial seryl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.40">
        <property type="entry name" value="Serine-tRNA synthetase, tRNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002317">
        <property type="entry name" value="Ser-tRNA-ligase_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042103">
        <property type="entry name" value="SerRS_1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00414">
        <property type="entry name" value="serS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11778">
        <property type="entry name" value="SERYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00981">
        <property type="entry name" value="TRNASYNTHSER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="tRNA-binding arm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="10">
        <location>
            <begin position="241"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="107"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="136"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="122"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ82561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="527" mass="57943" checksum="B7115F802B437DB5" modified="2010-06-15" version="1">MARNLFIYRPGASSTAWVCSNCRPRPMRRSRCFSIKPDLQPPPSAPKPTISVKSIREFPEYHRQNCLNRNYPSLADAPERIIALHGEWIGLQKGLRGDKERQNTLSAQLRSRNPLARGPHGGGDKTEVAAEGQEAKKSLLAEAKDVKAKISEVEERERVLENEMKSLAIQLPNWTHKESPIGTEPKLLGYINPELAPPSQRTGPRISHIQIGAELGLLDFSAAGTVSGWGWYYLLNEAVLLEQALVNYALSFAMKRGWKLVTPPNIVYSHMAAACGFRPRDQHGEQQIYTLDGAEGKIEHCLAGTAEIPLAGMYANKTFAEDQLPVKLVGVGRAYRAEAGARGAESKGLFRVHEFTKVELFAWGPPGQEEFSTPNSEPITATIITSASLFEEMLDLQKSIISSLGLHARILEMPTTDLGASASRKIDIEAYMPSREAKDPWGEISSLSNCTDYQSRRLNTRVKIAKPGAAGGVKSEFPWTLNGTGVAVPRVLMAILENGWSEERGGVVVPEVLRGYMGGLELIGRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDK3</accession>
    <name>D5GDK3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00001058001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430147">
        <property type="protein sequence ID" value="CAZ82596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838405.1">
        <property type="nucleotide sequence ID" value="XM_002838359.1"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GDK3"/>
    <dbReference type="GeneID" id="9181601"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001058001"/>
    <dbReference type="eggNOG" id="ENOG502QSDS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000374_2_0_1"/>
    <dbReference type="InParanoid" id="D5GDK3"/>
    <dbReference type="OMA" id="DNRIDYM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035361">
        <property type="term" value="C:Cul8-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019021">
        <property type="entry name" value="Mms22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28122">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SUBSTRATE RECEPTOR MMS22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28122:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SUBSTRATE RECEPTOR MMS22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09462">
        <property type="entry name" value="Mus7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="498"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="590"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="637"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="788"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2033"/>
            <end position="2055"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="223"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="286"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="315"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="363"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="532"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="551"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="2033"/>
            <end position="2046"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="2149" mass="241174" checksum="E76DAD670D7D2510" modified="2010-06-15" version="1">MTNWRETGYVPASDDDISDGELSTQEELLGLSEPRTPLNLYQGDSPATSSPLSSPPSVVLTPPSISAVRSPPRRVTFSVVIPAAEGREEEEIIIPPDNDAPQYYRSLRVRKPIQRNPYLIEQERYRQTLKARGVRPVRISEVEEETNGGLGADEDSQERDYVAPPEPENDESQFVPVSSRGRGGPGGQGDRPVDIYDFEGFQDAASASRERPRPNGALADGVKRRKVNHTYSKRPHASAVRPQPDAGQSAGNARLTPSSIQAEIDENGRDVYELSEDSPQRRSRTPLVQTSPNGSPTKKTPRQPTQEQGIKRRGILSLLDSSSDIESSPTGQGRPRPISPGSDDDTPRARPVRTVLELSSSPPEEEPTPSESEDESQEVRRLQRKTAGVLPASWWKLDKRQHPSSTNDSRRDRRSSTPVQDLTPRPGVARARITSRPRSPVGDAFLFGDDSDDSNGPANIQPQAPPSGRPRLLHRQHQIEVIEIDDVVEDDRIDRMLPVTQRNSGLRKQKQRNQNGPSRAPQGMSADRLSGARNSGSGSRLRQPRITNHIGTKRNSTKRKPKVPQLGIVDAVRHYRATNPEISPPTFLKVATRSARERRSKGRQGPSKKIFQLDTAADTQEVQSVLREWREGTIAIRAHDSPDEDRTDAPPTRPLSPTVEQHPAGVLGLDLGLSSIGASASRRRPKQTTLTNIMRTRPLLSRINDAHRLRQKSRKRSNVTAVPARPNAITHQPFFMPEPTQFEREEPPGIAVARLQKSSRQGQRLNLAELLQTTARRHQNLRRFMDDEDLINPPPIMRGDPRPAPTSTLKIVKPTRPRRKRTPARIDAETIERRQPPPEDRIIADDVEIEMIPEPAPNTTLDVKPVLIGLLPYGSKYTLSFDTIPLKPGTIFNSETFIGCGGLSKALNSKLQTLGHSSNFRFGDKLVRWGVYEDSMAAEFESIMHDIVEFVDGAHKGGSDVEAHMWASRAYMFYLFFSNYLYRVISFSDSIDLISFGQRILQAIDTSCDQVLPVLEGSTPLSLSKAPTRLALQLHVFGLLFAFQIYKLASGDPGARERLELDRTIRRLGHQLLGRLLRCGFNAIRECYEDQRVIANYERGIDPKHYLIEAWVIAIYTLDSVHYQHISFWQLLNNELRPPEIERALDIRIFEKHWRRVWTILPLYQFDEFGVVQGPVRDGNLSENWALLRVLIGRPLKVYGTNIEGHSGAINAYCRTLYARCHHLIAKWGWLNTDIIIPVLYEFFASKNLANLRNEETYGSPDFLQNLDRNPSLEMRDSDPCFHLLLKVVGLGLHAMKASTPINKIGNLVNRLMPNHGRQYLKYEELRVENLTALRNHHDLLSTLWWASPPQCRPPLEAIRYLVDPDLSHQQACIVSIRAWLNLMRFQLHSGEGLDVLKPFMGWFDHFTGSALNQHHAARNEAEKHFSAAKERGDTELTEDIMEDNIRRNQQQLEGILIDALKSLRSTLVGIKGQTQSAIVILTKGVASTANVLNSYGRLSNKLIFEALDLIQQYLVVTKSADESAGVSVQAGEDSQDYGDWSGFDDIIIKDDQRAAAEQLFVTVYEALHRLLSNCFGSDIQPEDSFLIKTVDTWALVVGFLVRHGLKNWDAYLDQYNPESWSSLRDTEQTRKFTAYFIPKVIQSAPDVYESNKTAFISFWLKTLVERESLLKFQNEYTATLLNCDPTNPILANPPFVRDEVSGEFKVTLTDFRARRLSLISTILANMRENYEETHNDSAQRALTLKLEYSEMLKSMMRSMKEIYQVLYPHTHAHVILISKQEIHAKSSSGAYVKFVQQVVEYLQQHSIDIVTIDPFFTDSSVFPLPSADPMYVVGRLKNYGLKLSHQRMHKQLTSFFQSVCERAAIDGQQEYLVHQLTTALSGELEVGPAQKPSLRSLFTEAIFPAYIECTFTNPAGWILSAPLLQATEKILAKLRLDVDSTLDACVEAVTSMLTTLLNSIHASLSALPTPDILSSSVNTAIYSLFLKVLTRAIPLLDTISPTPHHPAYETAKSMTTHAAAIQQHISNPAVPLLPLPPLPNPPPGPHTSTKSTTTATLTKTLRTEWKMHNGIWYVTRPASRREVLVPKFGVEVEELGGVVDGLFRVAARSEWMGGLVRGLDLRVWREENMGRGRMGICGRVGGVGGVFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDR0</accession>
    <name>D5GDR0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00006231001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WHI5/NRM1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430153">
        <property type="protein sequence ID" value="CAZ82653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838462.1">
        <property type="nucleotide sequence ID" value="XM_002838416.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDR0"/>
    <dbReference type="STRING" id="656061.D5GDR0"/>
    <dbReference type="GeneID" id="9181658"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006231001"/>
    <dbReference type="eggNOG" id="ENOG502RZ4H">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025591_0_0_1"/>
    <dbReference type="InParanoid" id="D5GDR0"/>
    <dbReference type="OMA" id="KTQRGWE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013734">
        <property type="entry name" value="TF_Nrm1/Whi5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40468:SF1">
        <property type="entry name" value="TOPOISOMERASE I DAMAGE AFFECTED PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40468">
        <property type="entry name" value="YALI0A15257P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08528">
        <property type="entry name" value="Whi5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0678">Repressor</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="66"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="212"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="293"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="76"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="128"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="164"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="227"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ82653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="321" mass="34899" checksum="5168C03A29D80826" modified="2010-06-15" version="1">MSSLPAPKPWTEKQTKEVIRKVIRRAEVSRIARLLQHRLALANFKTKNGWENLTLDTIEPKVEEDLRRKRQRGSQEKSPSPGTSFFSEGDLEWLRKNKANVQQKRRGSQSSGIGPLPNFRNSRKRVRTSSSAEFQQPSSKRRQSWRDQNRTVQSSPGYRYGTGEGKDGLEHGFHSSPPRTPPRRIAKPGNANKPAGDRGADLLIYLATSPSPAGVMPKAKFTPTTPPSNSSALPSSVMGTPGAPQTPQTGFNFADFVNITPSPAQGAWGSVGAGRGTPIANARKRLNFDQLHPPTGSPSVPAGNSRHTAGLGMELGGELVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDR6</accession>
    <name>D5GDR6_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">pectin lyase</fullName>
            <ecNumber evidence="8">4.2.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00006239001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Pectinolytic enzymes consist of four classes of enzymes: pectin lyase, polygalacturonase, pectin methylesterase and rhamnogalacturonase. Among pectinolytic enzymes, pectin lyase is the most important in depolymerization of pectin, since it cleaves internal glycosidic bonds of highly methylated pectins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Eliminative cleavage of (1-&gt;4)-alpha-D-galacturonan methyl ester to give oligosaccharides with 4-deoxy-6-O-methyl-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.</text>
            <dbReference type="EC" id="4.2.2.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the polysaccharide lyase 1 family.</text>
    </comment>
    <dbReference type="EC" id="4.2.2.10" evidence="8"/>
    <dbReference type="EMBL" id="FN430153">
        <property type="protein sequence ID" value="CAZ82659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838468.1">
        <property type="nucleotide sequence ID" value="XM_002838422.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDR6"/>
    <dbReference type="STRING" id="656061.D5GDR6"/>
    <dbReference type="GeneID" id="9181664"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006239001"/>
    <dbReference type="eggNOG" id="ENOG502QXM6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021980_0_1_1"/>
    <dbReference type="InParanoid" id="D5GDR6"/>
    <dbReference type="OMA" id="NGASWCT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030570">
        <property type="term" value="F:pectate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047490">
        <property type="term" value="F:pectin lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002022">
        <property type="entry name" value="Pec_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045032">
        <property type="entry name" value="PEL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31683">
        <property type="entry name" value="PECTATE LYASE 18-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31683:SF67">
        <property type="entry name" value="PECTIN LYASE F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00544">
        <property type="entry name" value="Pectate_lyase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00656">
        <property type="entry name" value="Amb_all"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0964">Secreted</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Pectate lyase" evidence="10">
        <location>
            <begin position="35"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00036818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ82659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="321" mass="33482" checksum="004164D9AE039594" modified="2010-06-15" version="1">MLKKEYNFIGSQGTVTETGCRPASNTCPNSGGQDAINGASWCTKSGYPGISVTYDKAGTTFIDVKSNKSIVGKGTAGVIKGIGLRIANGVSNVIIQNVHFTYLNPQYIWGGDAITLAGSDMFSLIGRQMIVTGYSKAGRVTISNNELDGNTSWSATCNGQHYWTMLFLGSSDLITLTGNYIHDCSGRAPKVGGASTSNVVVHAVNNYFSNIGRHSFDLETGGMALIEGNVFESVTTTITSTSVSAGGRIFNVYSASDANTCTASLGRSCVQNSVSGSGYWKAYTTSSFLTKFSGNNVQPASPVGGVKASVMSNAGIGHIGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDT5</accession>
    <name>D5GDT5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00006263001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430153">
        <property type="protein sequence ID" value="CAZ82678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838487.1">
        <property type="nucleotide sequence ID" value="XM_002838441.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDT5"/>
    <dbReference type="STRING" id="656061.D5GDT5"/>
    <dbReference type="GeneID" id="9184473"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006263001"/>
    <dbReference type="eggNOG" id="KOG2758">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1696790_0_0_1"/>
    <dbReference type="InParanoid" id="D5GDT5"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016650">
        <property type="entry name" value="eIF3e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10317">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21357">
        <property type="entry name" value="EIF3E_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="4">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ82678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="155" mass="17347" checksum="B8E7211587A374DD" modified="2010-06-15" version="1">MWPRRSSPTVTVRTIAASTRNSSRTLSALSSRSCMNIMTPSPIFEAEEVLGSDFFLASSAASFVDSARHLISESYCKIHQRIDIKDLAQRLNLTQDEGEKWIVNLIRDTRVDAKIDYKDGTVIMNHPPQSVYQQVIEKTKGGLFRTQVLTAAVSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5GE35</accession>
    <name>D5GE35_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00006357001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430164">
        <property type="protein sequence ID" value="CAZ82778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838587.1">
        <property type="nucleotide sequence ID" value="XM_002838541.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GE35"/>
    <dbReference type="FunCoup" id="D5GE35">
        <property type="interactions" value="127"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GE35"/>
    <dbReference type="GeneID" id="9183110"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006357001"/>
    <dbReference type="eggNOG" id="ENOG502QQBW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033072_0_0_1"/>
    <dbReference type="InParanoid" id="D5GE35"/>
    <dbReference type="OMA" id="CSEEHWA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046824">
        <property type="entry name" value="Mss51-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032717">
        <property type="entry name" value="Mss51_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28069">
        <property type="entry name" value="GH20023P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28069:SF1">
        <property type="entry name" value="PROTEIN MSS51, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20179">
        <property type="entry name" value="MSS51_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13824">
        <property type="entry name" value="zf-Mss51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mitochondrial splicing suppressor 51 zinc-finger" evidence="2">
        <location>
            <begin position="113"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="Mitochondrial splicing suppressor 51-like C-terminal" evidence="3">
        <location>
            <begin position="275"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ82778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="482" mass="55246" checksum="5D246911EAE53168" modified="2010-06-15" version="1">MRAHICQKCLAPSKFLLLHNTNTTTSPLIRRALPAITARRTFLTAFRKTTVEKDGEVRPPLPSESASPSDHSLYERSEGTRVLLKPNNLFHPMDESPSPSMRERAANIKRTAYCPHPHHRAASSPSHIKFTCPDCGIPAYCSEEHWAEDYQNHMLICDALREANEDDHDLRSGRFFPEFEYPGEQMDEALINFTNWDTLLYTRDYKAINDERSLRQVTKLLTYPITVASFMHELSPYDMRHRLTMEGLKSVSALRYTLHPPRVGAGKDIKGIRVTPPPVRLFILGARSESSLPREVWHQLTHMFPLSTFHLVFIGPQSMMNRDAELPLPDRTPANPFGAVVERVSHNMKVSTFVEYYHTLHDTGIFNPYDPYFDMFVLFHPGLGHPGSAAEWESTLPRLLDTKCAILCTGYTEEDMNRDVDWVHDKCKGEFDVLLEPGENKFRSMRWDINDGDPSELSQGNWGIWSFRGKRYEAVKQDIGTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEB9</accession>
    <name>D5GEB9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Casein kinase II subunit beta</fullName>
            <shortName evidence="4">CK II beta</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00001258001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 4">Regulatory subunit of casein kinase II/CK2. As part of the kinase complex regulates the basal catalytic activity of the alpha subunit a constitutively active serine/threonine-protein kinase that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Tetramer composed of two alpha chains, one beta chain and one beta' chain.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the casein kinase 2 subunit beta family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430173">
        <property type="protein sequence ID" value="CAZ82862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838671.1">
        <property type="nucleotide sequence ID" value="XM_002838625.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEB9"/>
    <dbReference type="STRING" id="656061.D5GEB9"/>
    <dbReference type="GeneID" id="9185517"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001258001"/>
    <dbReference type="eggNOG" id="KOG3092">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_442269_0_0_1"/>
    <dbReference type="InParanoid" id="D5GEB9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005956">
        <property type="term" value="C:protein kinase CK2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034456">
        <property type="term" value="C:UTP-C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019887">
        <property type="term" value="F:protein kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006359">
        <property type="term" value="P:regulation of transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1820.10:FF:000005">
        <property type="entry name" value="Casein kinase II subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.20:FF:000001">
        <property type="entry name" value="Casein kinase II subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1820.10">
        <property type="entry name" value="protein kinase ck2 holoenzyme, chain C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016149">
        <property type="entry name" value="Casein_kin_II_reg-sub_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035991">
        <property type="entry name" value="Casein_kinase_II_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000704">
        <property type="entry name" value="Casein_kinase_II_reg-sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11740">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11740:SF0">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01214">
        <property type="entry name" value="CK_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00472">
        <property type="entry name" value="CASNKINASEII"/>
    </dbReference>
    <dbReference type="SMART" id="SM01085">
        <property type="entry name" value="CK_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57798">
        <property type="entry name" value="Casein kinase II beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01101">
        <property type="entry name" value="CK2_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="245"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="549"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="31"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="52"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="246"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="274"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="558"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="609"/>
            <end position="618"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00045899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00062110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ82862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="618" mass="67259" checksum="AF896866A8B84A8E" modified="2010-06-15" version="1">MPQTPPPTSTDNNREQLGLNYPDARHYQPLPDSPDSPPTPPPQSEARKQQRHQQSQSNLLGHSTNLHSSPAHKQGPVRERVGSVRVEGSEVPLLVSSVTGGAFVAEDPTHSGSDLLVPEDGDDHSRTYTRAASAVATASTSATTTTVTTPYACPPIIPDPLGEDGEDNDEFYNLAALRSAGLYRRSGALDKQGRGQVPTGLVRREIGIVKRDEFRDYFRRDGDNSSSAALGSGRLKNVELAGRYAQTRSPPQHPTSTTLQASPVHSHSSGQSASEEEEISEVEEEEEEEEEEDDMEEEESWIGNFCSMLGHEYFAEVTEDFIEDDFNLTGLGQMVPMYKEALEMILDVEPEDDSDIASDLSVIESSAEILYGLIHQRFITSRQGIQMMYDKYLANHFGFCPRVYCIQARVLPCGYSDTPGVETVKLFCPSCCDIYVPPNSRFQTVDGAYFGTTFASLFLMTFPELDTSGNGRKEVLSGTSLNSVGGKKGVSQASAPSINGVLAANLAPGLGRGKQYEMRIYGFRVSERARSGPRMGWLRSRPEDVSLLDETAKSGATMDEEVEYSSEEEGLEDSEGEEESADEDEMALGGDSAVGSRLGTSRRGGITPMDTNGTGIAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEJ6</accession>
    <name>D5GEJ6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00006522001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430175">
        <property type="protein sequence ID" value="CAZ82939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838748.1">
        <property type="nucleotide sequence ID" value="XM_002838702.1"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GEJ6"/>
    <dbReference type="GeneID" id="9181570"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006522001"/>
    <dbReference type="eggNOG" id="KOG0565">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0566">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_315021_0_0_1"/>
    <dbReference type="InParanoid" id="D5GEJ6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004439">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046985">
        <property type="entry name" value="IP5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000300">
        <property type="entry name" value="IPPc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200">
        <property type="entry name" value="INOSITOL 5-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200:SF300">
        <property type="entry name" value="TYPE II INOSITOL 1,4,5-TRISPHOSPHATE 5-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22669">
        <property type="entry name" value="Exo_endo_phos2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00128">
        <property type="entry name" value="IPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inositol polyphosphate-related phosphatase" evidence="2">
        <location>
            <begin position="46"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="288"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ82939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="928" mass="102490" checksum="F17D6819DF3813A8" modified="2010-06-15" version="1">MSVIIVPSGTAGFNLPSPAAYLHQQDIHTYLSAQVLSRRNEYLQQKKIRVKVASWNVAACDGTERDLEEWLLDHQNDFKSEGDEGASEVGIYVLALQEVVDVTTTESFIKYMDPKISLKWEAHAQAAVPAGYICRASKQLIGLLLMVFVSPDLSPLVTSVSTAVVGTGMGVRATGFMGNKGGVGIRLVIGETLRLVLVDCHLAAFTNNTERRNWDAGEISERMSFDPVEKNFVGFDKGEKGDPSDDSGSALEGIEDADILLWCGDLNYRVELPNEDIRRLLSSYMPKDLPPTHADGPSTSASPVDQVQGEFEEPPLPPLPSEIHPEAVPTLQGTIDSLLNHDQLYKQQKDAKAFVGFREGKIRFLPSYKYDIGTVGRWDSSVKARAPSWCDRILWKVKGAEPQEAKRGRARSRGMSILKDEVLFQTEDSDEEDIVVSRADSAIPHVSSVSQAKTPTSVFRLPSGEIGLRQLYYTSCQNVTSSDHKPVVSMFEMWFPSVVPEMKAKVHAEVAREVDKMENERKPAVTVVVDQFDGGECEEGVVDFGEVRYNQAKRRSVTVANTGTAGANVRFVGRPSPDEGEGRGVVAKEWLKVQFSGGGASNNTLHGMLRFVLSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQVRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFD1</accession>
    <name>D5GFD1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Alternative oxidase</fullName>
            <ecNumber evidence="16">1.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">GSTUM_00006837001</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes cyanide-resistant oxygen consumption. May increase respiration when the cytochrome respiratory pathway is restricted, or in response to low temperatures.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="16">Binds 2 iron ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the alternative oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.-.-.-" evidence="16"/>
    <dbReference type="EMBL" id="FN430208">
        <property type="protein sequence ID" value="CAZ83224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839033.1">
        <property type="nucleotide sequence ID" value="XM_002838987.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFD1"/>
    <dbReference type="STRING" id="656061.D5GFD1"/>
    <dbReference type="GeneID" id="9188080"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006837001"/>
    <dbReference type="eggNOG" id="ENOG502QSB5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041974_3_0_1"/>
    <dbReference type="InParanoid" id="D5GFD1"/>
    <dbReference type="OMA" id="VHTYTRA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098803">
        <property type="term" value="C:respiratory chain complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009916">
        <property type="term" value="F:alternative oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010230">
        <property type="term" value="P:alternative respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01053">
        <property type="entry name" value="AOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1260.140:FF:000002">
        <property type="entry name" value="Alternative oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.140">
        <property type="entry name" value="Alternative oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002680">
        <property type="entry name" value="AOX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038659">
        <property type="entry name" value="AOX_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31803">
        <property type="entry name" value="ALTERNATIVE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31803:SF3">
        <property type="entry name" value="ALTERNATIVE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01786">
        <property type="entry name" value="AOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16" id="KW-0249">Electron transport</keyword>
    <keyword evidence="12 16" id="KW-0408">Iron</keyword>
    <keyword evidence="14 16 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11 16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 16" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5 16 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="142"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="204"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="355"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="372"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00025285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAZ83224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="383" mass="42794" checksum="3E326C929FA90F5C" modified="2010-06-15" version="1">MLSATTIRASAPAILATKHLPLASGIRVASCGLLHGVQTRKFTGSTNSFFLATTPKSKQERPLKAFRERSFPHPVYTEAQMNDIKIAHRKAKTWGDWVALAAVRVMRKCFDLATGYKHDSEIASGNKTPYGTNAFHMTPQKWLARFIFLESIAGVPGMAAGMIRHLNSLRRLKRDNAWIETLLEEAYNERLHLLTFLHYRQPGLFMRTMILGAQGIFFNLFFISYLVSPRTCHRFVGYIEEEAVITYTRAIADIENGRIPEWENLLAPDIAIKYFGLGPDANMLELLKVIRADEAKHREVNHTLANLSQDEDPNPYALIYEDGKPHPTKGLDFMKPEGWEKGDIDGGVVEEYMKPGKEWKTGGKPSGGAPTGSGTTTTTSQVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFV8</accession>
    <name>D5GFV8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Alpha N-terminal protein methyltransferase 1</fullName>
            <ecNumber evidence="5">2.1.1.244</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Translation associated element 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">X-Pro-Lys N-terminal protein methyltransferase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00007109001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N-terminal L-alanyl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-methionine = N-terminal N,N,N-trimethyl-L-alanyl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:54712"/>
            <dbReference type="Rhea" id="RHEA-COMP:13785"/>
            <dbReference type="Rhea" id="RHEA-COMP:13971"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:138057"/>
            <dbReference type="ChEBI" id="CHEBI:138315"/>
            <dbReference type="EC" id="2.1.1.244"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N-terminal L-prolyl-L-prolyl-L-lysyl-[protein] + 2 S-adenosyl-L-methionine = N-terminal N,N-dimethyl-L-prolyl-L-prolyl-L-lysyl-[protein] + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:54736"/>
            <dbReference type="Rhea" id="RHEA-COMP:13787"/>
            <dbReference type="Rhea" id="RHEA-COMP:13974"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:138059"/>
            <dbReference type="ChEBI" id="CHEBI:138318"/>
            <dbReference type="EC" id="2.1.1.244"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>N-terminal L-seryl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-methionine = N-terminal N,N,N-trimethyl-L-seryl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:54724"/>
            <dbReference type="Rhea" id="RHEA-COMP:13789"/>
            <dbReference type="Rhea" id="RHEA-COMP:13973"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:138061"/>
            <dbReference type="ChEBI" id="CHEBI:138317"/>
            <dbReference type="EC" id="2.1.1.244"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. NTM1 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.244" evidence="5"/>
    <dbReference type="EMBL" id="FN430220">
        <property type="protein sequence ID" value="CAZ83401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839210.1">
        <property type="nucleotide sequence ID" value="XM_002839164.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFV8"/>
    <dbReference type="FunCoup" id="D5GFV8">
        <property type="interactions" value="461"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GFV8"/>
    <dbReference type="GeneID" id="9181746"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007109001"/>
    <dbReference type="eggNOG" id="KOG3178">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055356_3_1_1"/>
    <dbReference type="InParanoid" id="D5GFV8"/>
    <dbReference type="OMA" id="PVRMYCL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071885">
        <property type="term" value="F:N-terminal protein N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000025">
        <property type="entry name" value="N-terminal Xaa-Pro-Lys N-methyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008576">
        <property type="entry name" value="MeTrfase_NTM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12753">
        <property type="entry name" value="AD-003 - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12753:SF0">
        <property type="entry name" value="ALPHA N-TERMINAL PROTEIN METHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05891">
        <property type="entry name" value="Methyltransf_PK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016958">
        <property type="entry name" value="DUF858_MeTrfase_lik"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="12">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00043129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00082558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR016958-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ83401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="232" mass="25553" checksum="12A8401EC1EEFF8A" modified="2010-06-15" version="1">MAGQAPPDALIDAQKSLEYWNSVDATPNGMLGGFGSVSRVDLVGSRVFIAKLKLPGTPATLAKPRVADCGAGIGRITKGFLSKLNSGRVCVDIVEPVKKFTDQASVNLKEEIDDGRVGEIYNVGLENWIPEEGAYWVIWNQWCLGHLQDQQLVDYLERCKVGIVPGGVIVIKENIAPDPDDIYDEADSSVMRTDPKLRTLIKQAGLWVVRTEVQRGLPKQLYQVRSYALKPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFZ5</accession>
    <name>D5GFZ5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00001958001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430230">
        <property type="protein sequence ID" value="CAZ83438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839247.1">
        <property type="nucleotide sequence ID" value="XM_002839201.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFZ5"/>
    <dbReference type="GeneID" id="9181786"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001958001"/>
    <dbReference type="HOGENOM" id="CLU_2470709_0_0_1"/>
    <dbReference type="InParanoid" id="D5GFZ5"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ83438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="88" mass="9574" checksum="3D818CAAEFE7844A" modified="2010-06-15" version="1">MPVLPVGNFTGMILALPCPSSSANTGSHALEHKYSIVPYAMGNVAYGHGCRTYIIVLDMTGKTNLSDENARMVPWPQDYSAVSYHFIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>D5GG39</accession>
    <name>D5GG39_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00001983001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430238">
        <property type="protein sequence ID" value="CAZ83482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GG39"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001983001"/>
    <dbReference type="HOGENOM" id="CLU_3433199_0_0_1"/>
    <dbReference type="InParanoid" id="D5GG39"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ83482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="16" mass="1785" checksum="F0007A79FD769993" modified="2010-06-15" version="1">MFGQEIGMVRNGTECI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGD8</accession>
    <name>D5GGD8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007330001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430264">
        <property type="protein sequence ID" value="CAZ83581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839390.1">
        <property type="nucleotide sequence ID" value="XM_002839344.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGD8"/>
    <dbReference type="STRING" id="656061.D5GGD8"/>
    <dbReference type="GeneID" id="9183179"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007330001"/>
    <dbReference type="eggNOG" id="ENOG502SAUS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086044_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGD8"/>
    <dbReference type="OMA" id="YQRKGWG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13508">
        <property type="entry name" value="Acetyltransf_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="81"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="232" mass="25741" checksum="8F08435AE872FF55" modified="2010-06-15" version="1">MAFIREYQEGDFDDIVEICKLTAHPSTHSSADILPYIYAIPYIRLEPIHAFVLDAGSSDSSSGRRVVGYIIGTPDTNTFAERYKEAYLPTLPPAIANTTTADTPATAQADDGDGDELRRMFGRILLNPEDMLHPATPDFIPCYPAHLHINILPGFQSKGFGGKLMERFLRGLRARETGVCGLHLGMRRDNVAAGRFYERCGFRLHEELSDEHTALMVKGLEEVGGEGAKNQW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGE4</accession>
    <name>D5GGE4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007340001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430264">
        <property type="protein sequence ID" value="CAZ83587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839396.1">
        <property type="nucleotide sequence ID" value="XM_002839350.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGE4"/>
    <dbReference type="GeneID" id="9183185"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007340001"/>
    <dbReference type="HOGENOM" id="CLU_2980784_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGE4"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="58" mass="6317" checksum="1D02E8030F158322" modified="2010-06-15" version="1">MAPLGLLTQLSQNLAPTRYGFSVSAFRPQGEGKDEEKGREGYQSINKPLSVVSCSNQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGP3</accession>
    <name>D5GGP3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007452001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430275">
        <property type="protein sequence ID" value="CAZ83665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839474.1">
        <property type="nucleotide sequence ID" value="XM_002839428.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGP3"/>
    <dbReference type="STRING" id="656061.D5GGP3"/>
    <dbReference type="GeneID" id="9185587"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007452001"/>
    <dbReference type="eggNOG" id="KOG2865">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_6_4_1"/>
    <dbReference type="InParanoid" id="D5GGP3"/>
    <dbReference type="OMA" id="PEDQFTN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05271">
        <property type="entry name" value="NDUFA9_like_SDR_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000358">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase 39 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051207">
        <property type="entry name" value="ComplexI_NDUFA9_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008030">
        <property type="entry name" value="NmrA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12126:SF11">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12126">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 39 KDA SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05368">
        <property type="entry name" value="NmrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NmrA-like" evidence="1">
        <location>
            <begin position="43"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="367" mass="41982" checksum="987C9FC14EC7E761" modified="2010-06-15" version="1">MRTQLFRVAPPMRRAIHDIAITRTGKPIIRTPGGGRSSIGGHTATVFGATGFLGRYIVNRLARSGCTVVIPYREEMAKRHLKVTGDLGRVVFMEWDMRNTPSIEASVRHSDIVFNLVGRDYTTKNFTYEDVHVEATQRIAEAIAKYDVDRFIQVSSFNADKNSPSKYYATKGQAEEDARAIFPETTIVRPGPMYGFEDRLLHRLAGTTNIFTANNMQQKMRPVHVIDVGKALEVIAYDDATASQTFELYGPRELTLKEIAHMVDKIVHKTHRHINLPKKAFQMIASVLDYLWWPTISPDEIERQHLDHKIDATAKTFGDLGIEPTDIEADMFQYIRHYRSAAYFDLPPMTEKEKRLERQYLHVQQDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGP8</accession>
    <name>D5GGP8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00007459001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430275">
        <property type="protein sequence ID" value="CAZ83670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839479.1">
        <property type="nucleotide sequence ID" value="XM_002839433.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGP8"/>
    <dbReference type="GeneID" id="9185592"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007459001"/>
    <dbReference type="eggNOG" id="KOG2504">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_1_0_1"/>
    <dbReference type="InParanoid" id="D5GGP8"/>
    <dbReference type="OMA" id="NWAVTRI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17352">
        <property type="entry name" value="MFS_MCT_SLC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF224">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="120"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="145"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="171"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="206"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="238"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="282"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="347"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="372"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="409"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="437"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="79"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ83670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="471" mass="51206" checksum="289AC0AF17C9C99A" modified="2010-06-15" version="1">MDKEQGPANSPPILVIPDGGCEDRNPQETDSSRVSSGKGEGYGDDVEIGPAREEFKNLEKQPATLPINDPSSFPDGGAAAWLCVLGGFAASFCSFGRFCIGIFQDYYEANQLKEYSPSTVAWILSLETFCMFFLGPVIGKYYDAYGSRVILVLGTFFHVFGLIMTSISSKYWHFLVCQGIVSSIGASLTFYPALGDVPTWFFKRRAFAFGVVASGSSLGGVILPIMIQRLIPRVGYGWTMRITAFLILGMMIIANLTLRSRLPPKGWTPFRPSDFLKHFKELGYSMSIAAAFLFFFGMFLPFNYIVVSARRNGMSVSLANYLVSVLNGTSIFGRILPGWLGDKFGRYNMMILTTGASGILVLALWLPTVGNLPTILFVCFYGFTTGAFVSLAPACLAQISDIREIGVRIGVMFATVSIAALTGVPIGGALIDRYDGGFLGLQIFCGVTLVASSICWALARWIVGGWKAARV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGQ3</accession>
    <name>D5GGQ3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00007464001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430275">
        <property type="protein sequence ID" value="CAZ83675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839484.1">
        <property type="nucleotide sequence ID" value="XM_002839438.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGQ3"/>
    <dbReference type="FunCoup" id="D5GGQ3">
        <property type="interactions" value="44"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GGQ3"/>
    <dbReference type="GeneID" id="9185597"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007464001"/>
    <dbReference type="eggNOG" id="ENOG502QPQM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030747_0_1_1"/>
    <dbReference type="InParanoid" id="D5GGQ3"/>
    <dbReference type="OMA" id="WSIYDAQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070916">
        <property type="term" value="C:inositol phosphoceramide synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006676">
        <property type="term" value="P:mannosyl diphosphorylinositol ceramide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03386">
        <property type="entry name" value="PAP2_Aur1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026841">
        <property type="entry name" value="Aur1/Ipt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052185">
        <property type="entry name" value="IPC_Synthase-Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31310:SF11">
        <property type="entry name" value="INOSITOL PHOSPHORYLCERAMIDE SYNTHASE CATALYTIC SUBUNIT AUR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14378">
        <property type="entry name" value="PAP2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="123"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="185"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="214"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="276"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="310"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="7">
        <location>
            <begin position="212"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="394"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="414"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ83675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="444" mass="49693" checksum="9907BABEE431F9DA" modified="2010-06-15" version="1">MKSPASKLFPGMLDTHPSFKKQSLMGWPSIPGFHRVRRKFASRMRSGQSPTSNLSTLQTSYDPADTIRALRAHRWSYWDGQYLFLVILGIFCLSIIETPGAIIKTLISTLLITSLILPITRQFFLPFLPVAGWLVLFYACRFIPSEWRPPIWVRVLPALENIIYGANLSNILSKNTNAFLDVLAWLPYGVIHFGAPPVCSAIMFIFAAPGTVPVFARSFGYMNIIGVAIQLMFPCSPPWYENLHGLAPANYSMPGSPAGLARIDELFGWNTYTSTFTAAPLVFGAMPSLHAGCATIEALFMGHVFPRGKWLFYTYILWIWWATLYLQHHYAVDLIAGSFLATCTFYVAKTKFLARLQPDKEFRWDYDFLEIGNGNSPNYAFGMSDFNGDCHSEDDEWTTGSSSSVSSGCRSPVEDTQSVWESGSETLASHSDTEGIDVVIDDRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5GH71</accession>
    <name>D5GH71_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00007776001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430308">
        <property type="protein sequence ID" value="CAZ83896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839705.1">
        <property type="nucleotide sequence ID" value="XM_002839659.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GH71"/>
    <dbReference type="STRING" id="656061.D5GH71"/>
    <dbReference type="GeneID" id="9182601"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007776001"/>
    <dbReference type="eggNOG" id="KOG0685">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004498_8_2_1"/>
    <dbReference type="InParanoid" id="D5GH71"/>
    <dbReference type="OMA" id="LTHAWIE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001716">
        <property type="term" value="F:L-amino-acid oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009063">
        <property type="term" value="P:amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050281">
        <property type="entry name" value="Flavin_monoamine_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742:SF382">
        <property type="entry name" value="AMINE OXIDASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742">
        <property type="entry name" value="FLAVIN MONOAMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072328" evidence="1">
        <location>
            <begin position="20"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="domain" description="Amine oxidase" evidence="2">
        <location>
            <begin position="180"/>
            <end position="655"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ83896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="683" mass="76622" checksum="80083743B25D5EDD" modified="2010-06-15" version="1">MTKYTFLSILLTAATSIIASPAPHPLHLEVRTAIDTGAANVHLIRRSLVSEGVVYTYGSCASFSSQDSHHFVSSTPLGVVADRLLWRVPEDAPPGGCISAWGMNGGSLLGRSLPLDVRPGLKKRNLERRQVNPVKMDNSSGIDTSGPWFDGVKLLKDKEVPPIVIEELKKKKIGILGAGISGLMTGLLLDSQGFHNWEITEASQRLGGRIHTEYFAGGPTTDDYQYQEMGPMRFPRSIKYAGTNETLIIRDHHIVFQLAEYLNNMNKGDANLVVKFITWIQTNPNTRSYFQGIRKPDGSVPTLAETQADPSLNPAPITDPSFKEVEDQLFNLYMTKEKMAELANNVYRAHKKAIDEGWDDFSEFEYIHTLLGASLNITDLAIPFPESFADSFWGDYMDNVYFSATDWVTIDHGLNRLPLAFEPIVKDRVHFGRRVDKISYSKSGNKVKFSWRKDFRDRDYLNAEYDYALVAAPFSIVRKLRTPAFSSVLGRAIGRLGYTNACKIAVQFKTRFWEHLDPPIFGGCTTTDIPLIQRYCYPSYNLNGTGPGVLLASYNFGGDADRSVSFTDDEHIEHVMAAMEEIHGPVVRQQFMRAERKCWLLDGLEAGSWAEPLIGQHKLYIPSYFNVENNIIFIGEHTSYTHAWIASALESAVRGTVQLLLEWGLVDEAKNITEAWMGRWITV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHU4</accession>
    <name>D5GHU4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">chitin synthase</fullName>
            <ecNumber evidence="2">2.4.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">GSTUM_00008119001</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>[(1-&gt;4)-N-acetyl-beta-D-glucosaminyl](n) + UDP-N-acetyl-alpha-D-glucosamine = [(1-&gt;4)-N-acetyl-beta-D-glucosaminyl](n+1) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16637"/>
            <dbReference type="Rhea" id="RHEA-COMP:9593"/>
            <dbReference type="Rhea" id="RHEA-COMP:9595"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17029"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="EC" id="2.4.1.16"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:16638"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.4.1.16" evidence="2"/>
    <dbReference type="EMBL" id="FN430321">
        <property type="protein sequence ID" value="CAZ84087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839896.1">
        <property type="nucleotide sequence ID" value="XM_002839850.1"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GHU4"/>
    <dbReference type="GeneID" id="9184180"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008119001"/>
    <dbReference type="eggNOG" id="KOG2571">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000192_0_0_1"/>
    <dbReference type="InParanoid" id="D5GHU4"/>
    <dbReference type="OMA" id="RLAEWAN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030428">
        <property type="term" value="C:cell septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004100">
        <property type="term" value="F:chitin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006031">
        <property type="term" value="P:chitin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000010">
        <property type="entry name" value="Chitin synthase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004835">
        <property type="entry name" value="Chitin_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014876">
        <property type="entry name" value="DEK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914">
        <property type="entry name" value="CHITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914:SF13">
        <property type="entry name" value="CHITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03142">
        <property type="entry name" value="Chitin_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08766">
        <property type="entry name" value="DEK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109715">
        <property type="entry name" value="DEK C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51998">
        <property type="entry name" value="DEK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="8" id="KW-0518">Myosin</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="703"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="739"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1008"/>
            <end position="1029"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1399"/>
            <end position="1426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1432"/>
            <end position="1453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1460"/>
            <end position="1483"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="15">
        <location>
            <begin position="768"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="domain" description="DEK-C" evidence="16">
        <location>
            <begin position="1684"/>
            <end position="1740"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="632"/>
            <end position="661"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAZ84087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1744" mass="193235" checksum="0C12BA8AF668B594" modified="2010-06-15" version="1">MSFSNRHSIYSTTSASPYGGNAAQTSGTVTTSSLLSTLHNAYQSSIPHALDASTTLVVNSSNSGGVGGYGTSVIDETLGTRAWEHARRRAEDQTILLGSLHSNSPSLILPILGTLPSPPDILNTSLALISAFTSCLTPSSPFPVPYHNGLAITLQISLSGTITGAHISISAAGLDTQNGLLRIPAQAGYRAFDVFYYLLSAASAPAERQGLGLQPADKYSLLARSGTYSLPSYLPTADDSAAAEDWRANLRAVGIKGSLLRGLLSALAGILKLGNAIGLLVDDEIIEEVCEDVSGLLGVEPEILAKKVGDGEREVFIAAVYEAIVEWIVAKANDAIAADFAARKDAESSSEDSQEGDTVQITVLELPNEKMAKALCLKGVFDDESGLNVEMKLDGVEIPSASGTVQREMRSSWAEAEKEGLVGNGREREYERDRRESVVEKTGRDAEEGGFLKELLFPTEFGRSPETVKFEATQLLAASRVWWHLSLFPSDDIPSGLQPVPWSASAVSRQLRSWRLPEWANRRTKHLDFTADFDFDEFAVRYSPLGCTSGRDGVESWVIERGWSNGEVVVGRERVWMREGSWWEAENMLDLKQPGVPPNMMGMGAGPVDTGYTQNTNGSGYFGPMGGMGMSPPGSTSQLLQNQGSMGGRGLPGSSPLAHNRDGSEQGALLSKYEQEHYAGKLDPEMATPKNVESVPTSKTRKIWVAVVWAMTWWIPGFVLRLVGRMKRPDVRMAWREKFVLFFFIFILNAAIVFWIAFFAKLLCPDYDRAWNRKEVGYHQGEGDFWVSIHGYVYDITSFWTLQHSDSNIKTTRDNMIDFAGLDLSPYFPPPINLGCPDLVQSNLINMQYNDTSAQRLVVGVHASGPRNAQDPTSKLHSADWYSQTFLPRIKEYRKGDLVWDRKKIKRDGVAFNKPWGIIDGKIYDLTDYLYTADQNPNSGAANVPNYNYLGSTLEDIFKNNPGSDVTKQFREQLNATAQTQTLRCLNNLFYVGKTDFRKTAKCQVNSYILPAFAGIISAVILAKFLAALQLGSKRRPAMQDKFVICQIPAYTEGEDQLRKALDSLTALNYDNKRKLLCVVCDGMIVGGGNDRPTPSIILDILGVDPKIDPPALPFKSVGEGSAQLNYGKVYSGLYEYEGCVVPYVVVVKVGKPSEHGKSKPGNRGKRDSQVLLLDFLNRVHHRTPMSPLQLEMFHQINNIIGVDPELYEYLLMVDADTSVREDSLNRLVAACANDAKIAGICGETSLENEERSWWTMIQVYEYYISHHLAKAFESLFASVTCLPGCFCMYRLRTPDKGRPLIISSKVIDEYSDGNVDTLHKKNLLSLGEDRYLTTLMTKHFPYMSYKFIPDAFAHTAAPETWSVLLSQRRRWINSTIHNLFELFFLKEMCGFCFFSMRFVVFLDLMGTIILPATCVYFGYLIYVIASKTGPMPILSLVMIAVVYGLQAIIFIIKRQWQHVGWMVIYILAFPIYSFILPVYSFWAQDDFSWGNTRIVIGESGDKKIVATEDEGFDPRSIPLQTWDDYAAQNSLPGRRGGAPEKFSSGYEDGVELDDIQSTYSSVKPASTILTGLPQMGGVHPYMPPQSPAFQNNRQSTYSAYTAFTGHPGDHNQHQRLMSMGGMSNRDYWQDGHGNRGAMSTGLPSTDNLMAMNTPPTRAPSRSPLDFPASRPVSQIDFMRAGGGVDDELIVEAIRAVLREVDLETVTKKQVRALVEQRLQTECQGERRTFLDRQIDTELANMPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHV8</accession>
    <name>D5GHV8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">L-lactate dehydrogenase (cytochrome)</fullName>
            <ecNumber evidence="17">1.1.2.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Cytochrome b2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Flavocytochrome b2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">L-lactate ferricytochrome c oxidoreductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">GSTUM_00008138001</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24 25">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>(S)-lactate + 2 Fe(III)-[cytochrome c] = 2 Fe(II)-[cytochrome c] + pyruvate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:19909"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16651"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.1.2.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:19910"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">In the C-terminal section; belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="16">In the N-terminal section; belongs to the cytochrome b5 family.</text>
    </comment>
    <dbReference type="EC" id="1.1.2.3" evidence="17"/>
    <dbReference type="EMBL" id="FN430321">
        <property type="protein sequence ID" value="CAZ84101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839910.1">
        <property type="nucleotide sequence ID" value="XM_002839864.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHV8"/>
    <dbReference type="STRING" id="656061.D5GHV8"/>
    <dbReference type="GeneID" id="9184194"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008138001"/>
    <dbReference type="eggNOG" id="KOG0537">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0538">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020639_1_1_1"/>
    <dbReference type="InParanoid" id="D5GHV8"/>
    <dbReference type="OMA" id="WSYVAGG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004460">
        <property type="term" value="F:L-lactate dehydrogenase (cytochrome) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02922">
        <property type="entry name" value="FCB2_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.120.10:FF:000009">
        <property type="entry name" value="Cytochrome b2, mitochondrial, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000062">
        <property type="entry name" value="Cytochrome b2, mitochondrial, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000262">
        <property type="entry name" value="FMN-dep_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037396">
        <property type="entry name" value="FMN_HAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037458">
        <property type="entry name" value="L-MDH/L-LDH_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578:SF82">
        <property type="entry name" value="CYTOCHROME B2, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G07200)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578">
        <property type="entry name" value="S -2-HYDROXY-ACID OXIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01070">
        <property type="entry name" value="FMN_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00363">
        <property type="entry name" value="CYTOCHROMEB5"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51349">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0288">FMN</keyword>
    <keyword evidence="6" id="KW-0349">Heme</keyword>
    <keyword evidence="12" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="22">
        <location>
            <begin position="32"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="FMN hydroxy acid dehydrogenase" evidence="23">
        <location>
            <begin position="137"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00052399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00061137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00061589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00066458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00068515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00075949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00078774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00078938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="CAZ84101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="524" mass="56650" checksum="190CD194ADED16A9" modified="2010-06-15" version="1">MLLNRDSTTADNCRQLPLTQRKVTASWTPLQTAIANSLQVAKHNTSSSCWVIIHNTVYDVTDFLSAHPGGAKAILSLAGQDATEEYDPIHPPGTLEESLEKSQILGPVDPSTIITITPTPSLSSSPSEKDKARDGIPPLAEQLNLDDFAITAEKVLSPKAWAYYFSASDDQLTKRHNNTAYQQILLRPRIFKDVRNVDTRTTMCSSSVAFPVFVAPAAMARLAHPSGEAGIAEACGREGVLQCVSTNASLKPEQVMAGRVSDKQPSWFQLYVQEDRRKSEALLKRVGTLGFTAVVLTLDAPTPGKREADERAKNAGNITSATFGESMQGKSESGGLGKALFAGTTPSLTWEDLEWLRKHTRLPIILKGLQTHEDAAMAARKEVLELGVTGIILSNHGGRAADTAPPPVYVLMEIRKYAPEVFDKLEVYVDGGIRRGTDVVKALCLGAKAVGIGRPALFGLSGYGVDGVRRVLAILREEIETTMRLLGVYSVGELGSRHINTRALDGLIYQEPQELSGGVIKAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>D5GI28</accession>
    <name>D5GI28_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008242001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430322">
        <property type="protein sequence ID" value="CAZ84171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839980.1">
        <property type="nucleotide sequence ID" value="XM_002839934.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GI28"/>
    <dbReference type="GeneID" id="9183504"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008242001"/>
    <dbReference type="HOGENOM" id="CLU_2198912_0_0_1"/>
    <dbReference type="InParanoid" id="D5GI28"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="108" mass="12042" checksum="317F97EE2FEAEAF4" modified="2010-06-15" version="1">MARGEFERAKRELVFDGVFTEEYFARGGVWLYAIAEQGEEGEGEVTLDRVVEMHPLVKRWVCRVEELARGIGVGVEELELERGVGREVEEGKGEEDGSEVGAEAEAQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GI53</accession>
    <name>D5GI53_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00008280001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the INCENP family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430323">
        <property type="protein sequence ID" value="CAZ84196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840005.1">
        <property type="nucleotide sequence ID" value="XM_002839959.1"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GI53"/>
    <dbReference type="GeneID" id="9181914"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008280001"/>
    <dbReference type="eggNOG" id="ENOG502S0AD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003318_1_0_1"/>
    <dbReference type="InParanoid" id="D5GI53"/>
    <dbReference type="OMA" id="PVPKWAQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005635">
        <property type="entry name" value="Inner_centromere_prot_ARK-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13142">
        <property type="entry name" value="INNER CENTROMERE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13142:SF1">
        <property type="entry name" value="INNER CENTROMERE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03941">
        <property type="entry name" value="INCENP_ARK-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inner centromere protein ARK-binding" evidence="9">
        <location>
            <begin position="1342"/>
            <end position="1401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="112"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
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            <begin position="185"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="718"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="796"/>
            <end position="1369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="113"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="185"/>
            <end position="194"/>
        </location>
    </feature>
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        <location>
            <begin position="225"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
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            <begin position="257"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="280"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="315"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="368"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="380"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="482"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="510"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="579"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="597"/>
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        </location>
    </feature>
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        <location>
            <begin position="620"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="718"/>
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        </location>
    </feature>
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        </location>
    </feature>
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        <location>
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        </location>
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        <location>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="1013"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1077"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1127"/>
            <end position="1139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1140"/>
            <end position="1152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1154"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1203"/>
            <end position="1215"/>
        </location>
    </feature>
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        <location>
            <begin position="1242"/>
            <end position="1254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1269"/>
            <end position="1279"/>
        </location>
    </feature>
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        </location>
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        <location>
            <begin position="1306"/>
            <end position="1319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1345"/>
            <end position="1355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ84196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1454" mass="159941" checksum="7F8AB8D3089B7530" modified="2010-06-15" version="1">MAGRPISKIAPVGSMTWITNERTTMRQLGGQDFEDFTFSARGELEWLNEHMADIFERSQVDMTELLKTPGRLRGKTPRTARQQLRQVEPFGDVFGANKPAAVLPAGDSRFHNALSQLPSNGSRVSPAREKSSGSKSPNRLLRKQQDVAPPPPQVDGTMRDSGIEFSQVEGPAAQVNDSGIWSASSEYASQQSVYPSRRKSTPVDVPAQEEAALPSPAPPSAPTSKSDERTTRQSFRTAHEDEASSERPHSPIQKAPKSPKKSPGKPKSRRVSPVKTISHSPERDAAPAPEPRRSGRIRSGRGSTGSELSNNENEVPPPSSPPATRPAPSQEETKSFTGKAAGDGYPQLPSEPTQAPEPAPAPAGTPIRHAEEAEKPPQPETQMTPASERSGSGSPEPVIRKSILNFASLPAREPLTSKKKSLGVRVTRESHLEQLRANGTSRASWMNRGGNGKSLGAANRSVDAVANSIEDRDEDGPVPVEKSLKRKSEQQETTRSRKKSKSINPVEVAARGEDSNGETEKDKVLHNKISTQRLHEKIQQLGKVKAPRETHSVSVLAAEPAYPDLSQLKQDQPPVPTRRPNSIHQGESTRPIADELPQEREKVGEKTRSESFTKPGLARSHTEPRLGVEEEPVRRPDSPKKTQTARVATLARTMSDVSAPPPRSPATRPRLSKSFSEVQHREPDLQSPLSPGGEGAISAVKTHTASVFKRAKEMLLKSSVSSASAKIESLSPAPRWQGINAMKQSTKTDTEAERSQHSAKSPVERLYPDLNSVTVAQQLLEEVSVENPVEKPVLVEEARQTRNSRSKEVAKEEAKKAGKVEERVAEEVALNEAAAVEPVMESRQTRNSRSRETPQEEVTEPAPPLMKSPRKLKNAAREEELRHVRELRRKHQEEMLRKEKEELRRRQQAEQQREKEEQRRKVIEVSSDSSSSSEDGEEEGAVDREVNRRRFGSSSEPERPPSRLRGAGGGFRKPAENKRPSRPAKEAPKSKPAPVNIRIGTASQREMQDQRSKMTAPTGSSLISALKSSFEPSLNASASQNSLQSIPSNSSMRSGSNTGNTGTSKQLKSLTAAANQLKKEQEERERKMAAKREIERRRQENQRRQIQEEEQKKMRQRPAQAVPPSAKTLSRIQSVANLRQKSEEPENKRLEYQTKATSFQKSNASKRALQQESSSDDLQNARLAPPKPGGPAYQQEGAKKRRTGEFEEERPRPAKEMAPPIRQSAVRGGLGQKSGLPHGYIPASQSASSSTAPSMLRPPTKPGSVPHVEGVKFSKEKLRFAPAEAPASASGPSSSDPPPNPTSHSTAFKTPGTSKSMRPTKTPAGVKESPIYQNGELIELPEIPTDSEDEDDDDDYASKDAFRVPDWAESPELRAQLQRQQTMNPEEVFGPMARLDMEAIFTNRNERQMARFRARTSSANWNGADRLTPAEIAADHEARLRMIEQGGWVYQPAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIR1</accession>
    <name>D5GIR1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="11">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00008619001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VTI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430328">
        <property type="protein sequence ID" value="CAZ84404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840213.1">
        <property type="nucleotide sequence ID" value="XM_002840167.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIR1"/>
    <dbReference type="FunCoup" id="D5GIR1">
        <property type="interactions" value="830"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GIR1"/>
    <dbReference type="GeneID" id="9185622"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008619001"/>
    <dbReference type="eggNOG" id="KOG1666">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075474_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIR1"/>
    <dbReference type="OMA" id="MEYEAND"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007036">
        <property type="term" value="P:vacuolar calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048280">
        <property type="term" value="P:vesicle fusion with Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15862">
        <property type="entry name" value="SNARE_Vti1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000002">
        <property type="entry name" value="Vesicle transport through interaction with t-SNAREsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.400">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038407">
        <property type="entry name" value="v-SNARE_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007705">
        <property type="entry name" value="Vesicle_trsprt_v-SNARE_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21230:SF26">
        <property type="entry name" value="VESICLE TRANSPORT THROUGH INTERACTION WITH T-SNARES HOMOLOG 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21230">
        <property type="entry name" value="VESICLE TRANSPORT V-SNARE PROTEIN VTI1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05008">
        <property type="entry name" value="V-SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12352">
        <property type="entry name" value="V-SNARE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="204"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="10">
        <location>
            <begin position="128"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ84404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="226" mass="25383" checksum="E2C49BE51E57A3CE" modified="2010-06-15" version="1">MSNTLDSDPGSELFASYEADFKLVRADISQKLDQIPELSGEPRKAAIRAAERAVDEADEILGQMSLEINNIPSAQRPKVKSRLRNYTHDLDTSRHSLVKHAQNFDRDALFGARSKPGTGDAVVDQRQQLLSGTDRLERSSQRLRDSQRVANETEEIGASILGDLGRQREQIMNTHNTLNDSERFLDRSIKTLRGMARRMATNRMITIAIITVLVLLIIAVIVSKFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJ00</accession>
    <name>D5GJ00_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">calcium/calmodulin-dependent protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">GSTUM_00008759001</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.17"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.17"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.17" evidence="2"/>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840302.1">
        <property type="nucleotide sequence ID" value="XM_002840256.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJ00"/>
    <dbReference type="FunCoup" id="D5GJ00">
        <property type="interactions" value="549"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJ00"/>
    <dbReference type="GeneID" id="9181834"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008759001"/>
    <dbReference type="eggNOG" id="KOG0032">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_63_0_1"/>
    <dbReference type="InParanoid" id="D5GJ00"/>
    <dbReference type="OMA" id="HDWFESR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004683">
        <property type="term" value="F:calcium/calmodulin-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000449">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000278">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="23"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="321"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="347"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAZ84493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="395" mass="44289" checksum="ACDC841F5C7A9BC5" modified="2010-06-15" version="1">MSFSSVLNKLHGQPPTYDKKSQYKMGRTLGAGTYGIVREADGPTGKVAVKIILKKNVKGNEQMVYDELALLQRLKHPHIVSFRDWFESRDKYYIVTQLATGGELFDRICEYGKFTEKDASQVVREVLDAVNYLHNNNIVHRDLKPENLLYLTPDAKSSLVLADFGIAKMLDSSDEMLNTMAGSFGYAAPEVMLKKGHGKAVDMWSLGVITYTLLCGYSPFRSESLPDLIDECSKGGVIFHERYWKEVSKDAKRFIGGLLQPRPEDRPTSQEALRHTWLIGETATDYDVLPEIKSYIAKARLRRGIEMVKLANRIEALKMRADDPEEDLPTDTGKAATEGVSSASQNTSTSGGARRLSRAARSAIFREVVLAKVRELKAEEAKQMAERTLTERSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJ11</accession>
    <name>D5GJ11_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00008771001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ESF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840313.1">
        <property type="nucleotide sequence ID" value="XM_002840267.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJ11"/>
    <dbReference type="FunCoup" id="D5GJ11">
        <property type="interactions" value="1052"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJ11"/>
    <dbReference type="GeneID" id="9181845"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008771001"/>
    <dbReference type="eggNOG" id="KOG2318">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010564_0_1_1"/>
    <dbReference type="InParanoid" id="D5GJ11"/>
    <dbReference type="OMA" id="DHDFAID"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039754">
        <property type="entry name" value="Esf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012580">
        <property type="entry name" value="NUC153"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056750">
        <property type="entry name" value="RRM_ESF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12202">
        <property type="entry name" value="ESF1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12202:SF0">
        <property type="entry name" value="ESF1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08159">
        <property type="entry name" value="NUC153"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25121">
        <property type="entry name" value="RRM_ESF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ESF1 RRM" evidence="7">
        <location>
            <begin position="148"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="NUC153" evidence="6">
        <location>
            <begin position="502"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="83"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="414"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="516"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="114"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="415"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="532"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="560"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="575"/>
            <end position="585"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF25121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ84504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="585" mass="67389" checksum="35560E922742F65F" modified="2010-06-15" version="1">MAKDKHRKKFSDEPKVPVTTDERFISVHNDPRFALPRRKDAKLKIDKRFERMYKDRSFAEKASVDRYGRNIGKDAGKEEIKRYYHISDEEDEDEEQARVYDPARGEGVISTSEESSDEEDEEVGEAEEEAARAQVECQREIPMGEVSKRFAAVNLDWDNVQAVDLLKTFSSFVTGGGRIVSVTVYPSEFGKERMEREEMEGPPTEIFKPKGKARVISSDDEDSNLDEEVDEKTIIKEDKGEEFDSSKLRNYQLERLRYFYAVVECDSLETARYIYEQCDGAEYEATANFFDLRFIPDETSFEDDKPQDRCVEVPANYKPVDFSTDALQHSKVKLTWDEDDQQRKLATKRAFSQRDLEDMDLKDYLASSDSESESGAREAAKDKYRALLSATGFGKKDKAELVGDMEVTFTSGLSEKAKDRKGDKRKDREAKSAEAAKNASKRAELELLMTGEDVDTDGKKLYHFDMKQIVKAEKKKGKKGRRRKAEFEPEGIQDGFEIDVKDPRFAAVYEKHEFAIDPTNPRFVKTKAMSKLLEERRNRTQKRNDDREEGEGERKRRKVDKADKGGKGEDELKRLVHSLKSRAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJ18</accession>
    <name>D5GJ18_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00008779001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840320.1">
        <property type="nucleotide sequence ID" value="XM_002840274.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJ18"/>
    <dbReference type="STRING" id="656061.D5GJ18"/>
    <dbReference type="GeneID" id="9181852"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008779001"/>
    <dbReference type="eggNOG" id="KOG2102">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1086624_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJ18"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033770">
        <property type="entry name" value="RRP44_S1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17215">
        <property type="entry name" value="Rrp44_S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exosome complex exonuclease RRP44 S1" evidence="2">
        <location>
            <begin position="184"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="64"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ84511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="256" mass="28216" checksum="49F0CB97BD924082" modified="2010-06-15" version="1">MRSTGIFLSSSWTPWQTNYLVEEFMPPANTSVAGKIYEAFSQTAMLRLANPPPHWPSTPNQLPTSKASKTNSKSARGLTRPICRPLQPFLQQTRAHPSDGFILSAEYFSSATRATLNSDTTFLRAGSTPTSHPPSTAIRTSFRTSNLWSHYTQNSTQKRNLSEYAERSTTDVATRRCAGRLESIFVRRVGVEGLICMRDLAPDEQKSAFGAEDYPFTVEGSAESRSVGLFGLVEVSAMGDVEECMGKGRIKMTLVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJ40</accession>
    <name>D5GJ40_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00008827001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840342.1">
        <property type="nucleotide sequence ID" value="XM_002840296.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJ40"/>
    <dbReference type="STRING" id="656061.D5GJ40"/>
    <dbReference type="GeneID" id="9181874"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008827001"/>
    <dbReference type="eggNOG" id="ENOG502SFMK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096545_1_0_1"/>
    <dbReference type="InParanoid" id="D5GJ40"/>
    <dbReference type="OMA" id="CCIAGCF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072825" evidence="2">
        <location>
            <begin position="19"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="132"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ84533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="180" mass="17228" checksum="7FFF6B1839732B91" modified="2010-06-15" version="1">MKFSAIILSLGFAALVAAQARNSTAQSAPTPTQTTSFSPQIAACLGACKEDDVTCRAKCLGSAAPNEAAVNATNTCIDKCPKGDGSEAANDQYSKCVTGCINTIYLSTATPAPTGGSGSGSGSGSGSGSGGNSANPTSDGGPSGTSSGSSSTSSPNAGSSVKVVGSFLTIFGIAAGVLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJA7</accession>
    <name>D5GJA7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00008914001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the isochorismatase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840409.1">
        <property type="nucleotide sequence ID" value="XM_002840363.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJA7"/>
    <dbReference type="STRING" id="656061.D5GJA7"/>
    <dbReference type="GeneID" id="9185968"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008914001"/>
    <dbReference type="eggNOG" id="KOG1211">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025343_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJA7"/>
    <dbReference type="OMA" id="VTTECCF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00431">
        <property type="entry name" value="cysteine_hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.850">
        <property type="entry name" value="Isochorismatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000120">
        <property type="entry name" value="Amidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000868">
        <property type="entry name" value="Isochorismatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036380">
        <property type="entry name" value="Isochorismatase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895:SF169">
        <property type="entry name" value="GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895">
        <property type="entry name" value="TRANSAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00857">
        <property type="entry name" value="Isochorismatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52499">
        <property type="entry name" value="Isochorismatase-like hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isochorismatase-like" evidence="3">
        <location>
            <begin position="24"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Amidase" evidence="4">
        <location>
            <begin position="280"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="86"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ84600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="724" mass="77698" checksum="BF0CA593ED97DF84" modified="2010-06-15" version="1">MEALSKNITIEAKPYPYTFPLSSTALLLIDLQRDFILPSGFGDIQSGTNLTAVTAVVPNCVRILQAFRELELPIFHTREGHLPDLSDCPSSKLGRQASAPGTSHSKVIGDPGELGRLLVRGEHGHDIVDECRPKLGEVVVDKPGKGAFWNTNLLEELVGWGITHLIVGGVTTECCVTTTVREANDRGFECCIIEECTAGYNDNFKAPSLNMIHWSQGLFGFVSSLPNFLKALTPALPSPGAPGPDLGLESPPSTPPVWDGCLTLDSLRKSYKSGLSPVTVITSLYARIEEYSQTKSVFIRLVDRPISISYAESLQKLFPDLTNLPPLYGVPFTLKDSINVAGIPTTLACPPLAHIPSRSSKIYSRLISLGAVYIGKTNLDQFATGLTGCRSPYGTPASIYNPDYVSGGSSSGSAVSVGAELSSFAIATDTAGSGRVPAGFNGVVGWKPTKGTVSFSGVMKACESLDCLSFMVTPDGGVKDVRKLWNLVRGYDPDDPYSKTPGSLPLPMVNALGEKKWKFALPDRKAVAECSPEYRKLFYQAIGSLQEIGGEVKEGDWGLFEEAGKLLYDGALVNERLAALPDNGWVGREKDELHPVIREILQNVLETGASAAEALTRKVNATLFNPSHPSYIDVLIVPTAPFHPRISAVLKDPIAINTRLGTFTHFGNVLDLCAIAVPAGHYMEDEKKMPFSITFLGRGGSDARVLEIASLFEGLVGVGAKDSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJC1</accession>
    <name>D5GJC1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00008933001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATPase g subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430330">
        <property type="protein sequence ID" value="CAZ84614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840423.1">
        <property type="nucleotide sequence ID" value="XM_002840377.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJC1"/>
    <dbReference type="FunCoup" id="D5GJC1">
        <property type="interactions" value="69"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJC1"/>
    <dbReference type="GeneID" id="9185982"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008933001"/>
    <dbReference type="eggNOG" id="KOG4103">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083674_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJC1"/>
    <dbReference type="OMA" id="NMTPPNL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015078">
        <property type="term" value="F:proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015986">
        <property type="term" value="P:proton motive force-driven ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006808">
        <property type="entry name" value="ATP_synth_F0_gsu_mt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04718">
        <property type="entry name" value="ATP-synt_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="4" id="KW-0138">CF(0)</keyword>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ84614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="200" mass="21295" checksum="E57FEBECB06F7739" modified="2010-06-15" version="1">MQTSLLRSPTIRQLASRRFASTTAESASQAASQAASKAQDAAKQTAARAQDAAKVAASKLISAASVLGNSLGKVGGRTGQLIKGVESLIPPTIYYAKVTKELSKLVFYGQRMSPPNVETFQKTFSPIIDMLKNPRALMNSVSTSPTLQPASILARLKEITPKEYAQAGVLTAEVIGFYTVGKMIGRRKIVGYRGGNGDHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJH2</accession>
    <name>D5GJH2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00009010001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430331">
        <property type="protein sequence ID" value="CAZ84665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840474.1">
        <property type="nucleotide sequence ID" value="XM_002840428.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJH2"/>
    <dbReference type="FunCoup" id="D5GJH2">
        <property type="interactions" value="419"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJH2"/>
    <dbReference type="GeneID" id="9182666"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009010001"/>
    <dbReference type="eggNOG" id="KOG1471">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014001_0_1_1"/>
    <dbReference type="InParanoid" id="D5GJH2"/>
    <dbReference type="OMA" id="QVMFNDF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.20">
        <property type="entry name" value="N-terminal domain of phosphatidylinositol transfer protein sec14p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051026">
        <property type="entry name" value="PI/PC_transfer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45657">
        <property type="entry name" value="CRAL-TRIO DOMAIN-CONTAINING PROTEIN YKL091C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45657:SF1">
        <property type="entry name" value="CRAL-TRIO DOMAIN-CONTAINING PROTEIN YKL091C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03765">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00180">
        <property type="entry name" value="CRETINALDHBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="2">
        <location>
            <begin position="107"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="303"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ84665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="323" mass="36053" checksum="AE24FD574072E39A" modified="2010-06-15" version="1">MTEQINAIIEPKLEPKYDHYDFPITNPMTGSGHSGHVDAEQNAKLFQLRSELESEGCTERLDTLTLLRFLRARKFDVPLAKAMFLATEKWRKEFEVDKIVSTFEYTEKPKVFEYYPQYYHKTDKDGRPVYIEQLGKIDLNAILAITTQDRMLQNLVLEYERLADPRLPACSRKAGHLLETCCTIMDLKGVGVTSIGSVYTFLKAVTAISQNYYPERLGKLYIINAPWGFSSAFSVVKAFLDPVTVDKIHILGSGYQAELLKQVPAENLPVIFGGTCSCEGGCELSDAGPWQEKQYTETVVKPTTTTTAAAPEPAPAAMATSTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJK2</accession>
    <name>D5GJK2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">3-hydroxy-3-methylglutaryl coenzyme A reductase</fullName>
            <shortName evidence="9">HMG-CoA reductase</shortName>
            <ecNumber evidence="9">1.1.1.34</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00009050001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(R)-mevalonate + 2 NADP(+) + CoA = (3S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:15989"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36464"/>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.34"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="9">Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Endoplasmic reticulum membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the HMG-CoA reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.34" evidence="9"/>
    <dbReference type="EMBL" id="FN430332">
        <property type="protein sequence ID" value="CAZ84695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840504.1">
        <property type="nucleotide sequence ID" value="XM_002840458.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJK2"/>
    <dbReference type="FunCoup" id="D5GJK2">
        <property type="interactions" value="267"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJK2"/>
    <dbReference type="GeneID" id="9182696"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009050001"/>
    <dbReference type="eggNOG" id="KOG2480">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001734_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJK2"/>
    <dbReference type="OMA" id="AGPMMID"/>
    <dbReference type="UniPathway" id="UPA00058">
        <property type="reaction ID" value="UER00103"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004420">
        <property type="term" value="F:hydroxymethylglutaryl-CoA reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015936">
        <property type="term" value="P:coenzyme A metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00643">
        <property type="entry name" value="HMG-CoA_reductase_classI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3270.10:FF:000001">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl coenzyme A reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.420:FF:000001">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl coenzyme A reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.770.10:FF:000001">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl coenzyme A reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.770.10">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl-coenzyme A Reductase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3270.10">
        <property type="entry name" value="HMGR, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.420">
        <property type="entry name" value="Hydroxymethylglutaryl-CoA reductase, class I/II, NAD/NADP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025583">
        <property type="entry name" value="HMG-CoA_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002202">
        <property type="entry name" value="HMG_CoA_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023074">
        <property type="entry name" value="HMG_CoA_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023076">
        <property type="entry name" value="HMG_CoA_Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004554">
        <property type="entry name" value="HMG_CoA_Rdtase_eu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023282">
        <property type="entry name" value="HMG_CoA_Rdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009023">
        <property type="entry name" value="HMG_CoA_Rdtase_NAD(P)-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009029">
        <property type="entry name" value="HMG_CoA_Rdtase_sub-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053958">
        <property type="entry name" value="HMGCR/SNAP/NPC1-like_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00533">
        <property type="entry name" value="HMG_CoA_R_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10572">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL-COENZYME A REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10572:SF24">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL-COENZYME A REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00368">
        <property type="entry name" value="HMG-CoA_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13323">
        <property type="entry name" value="HPIH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12349">
        <property type="entry name" value="Sterol-sensing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00071">
        <property type="entry name" value="HMGCOARDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55035">
        <property type="entry name" value="NAD-binding domain of HMG-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56542">
        <property type="entry name" value="Substrate-binding domain of HMG-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00066">
        <property type="entry name" value="HMG_COA_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00318">
        <property type="entry name" value="HMG_COA_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01192">
        <property type="entry name" value="HMG_COA_REDUCTASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50065">
        <property type="entry name" value="HMG_COA_REDUCTASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50156">
        <property type="entry name" value="SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0521">NADP</keyword>
    <keyword evidence="7 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="212"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="240"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="266"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="334"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="359"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="429"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="10">
        <location>
            <begin position="211"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ84695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1020" mass="109898" checksum="5AFE7521C4148576" modified="2010-06-15" version="1">MQLPLLPPSLTTKKPPPQWDPFTPMVTGLSKSACIHPIHTIVIIALLASTTYISLLENSLFERSGKNWFGCAKRGALLDGSVRVGLGEETQWKWKEIGNRAMEDKSAMELALLTLSFPVGSKFSVLVPSNSSAIALPSGLSSAVYAIPHSELNTFVDSVAELPAADGHDNQGRRRRWVQVGLDHTGRGWAEKIHESANSFIEHVKNAEPLDIAIVFLGYLSMHLTFLSLFMSMRRLGSNFWLATTVLFSSTFAFLFASIATHKMGVPINMILLSEGLPFLVVTIGFEKPIILTRAVLEASQKKARQAGSSNAAPIQAAIVAAVRDKGFEIVRDYIIEIIILSLGAASGVQGGLRQFCFLAAWILAFDCILLFTFYTAILVIKLEINRIKRHVALRKALEEDGVSRRVAENVEDAGVFGNRVRDSSVGKFKVLMVTGFVLVNIFNLCTLPFRDNSSSTVIATEKQPVNVDWTLAGASLESMISDKVPMVVESLLRSLEDPVLSKWIFVALAMSLVLNGYLFNAARWSVKETGHSREYEASTTANGLVDMAMAPSVETGNEVGRTLVECEEMLKEKRARELNDAELVELSLRGKIPGYALEKTLADTGRAVRIRRSVISRTPATMDKTGLLERSLLPHEHYDYDRVLGACCENVIGYMPLPVGIAGPLNIDGEIFYLPMATTEGVLVASTSRGCKAINAGGGAVTVLTGDGMTRGPVVEFPSVRRAGAAKNWIDSEEGQRRLKKAFDSTSRFARLQTIKTALAGTYLFIRFRTTTGDAMGMNMISKGVEYALHVMSTECGFDDMFIVSVSGNYCTDKKPAAINWIEGRGKSVVAEAIIPAAVVKSVLKSDVNALVELNISKNLIGSAMAGSVGGFNAHAANIVTAVFLATGQDPAQNVESSNCITVMKRTAEGALQISVSMPSIEVGTIGGGTILEPQGAMLDLLGVRGAHQTNPGDNARKLARIVAAAVLAGELSLCSALAAGHLVKSHMAHNRSAPTSRATTPVPTEKAPLAGLTMTTMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJT7</accession>
    <name>D5GJT7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00009187001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA repair metallo-beta-lactamase (DRMBL) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430333">
        <property type="protein sequence ID" value="CAZ84780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840589.1">
        <property type="nucleotide sequence ID" value="XM_002840543.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJT7"/>
    <dbReference type="FunCoup" id="D5GJT7">
        <property type="interactions" value="200"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJT7"/>
    <dbReference type="GeneID" id="9186816"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009187001"/>
    <dbReference type="eggNOG" id="KOG1361">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005260_4_0_1"/>
    <dbReference type="InParanoid" id="D5GJT7"/>
    <dbReference type="OMA" id="GLDANHC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035312">
        <property type="term" value="F:5'-3' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16273">
        <property type="entry name" value="SNM1A-1C-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12650">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011084">
        <property type="entry name" value="DRMBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23240:SF6">
        <property type="entry name" value="DNA CROSS-LINK REPAIR 1A PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23240">
        <property type="entry name" value="DNA CROSS-LINK REPAIR PROTEIN PSO2/SNM1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07522">
        <property type="entry name" value="DRMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA repair metallo-beta-lactamase" evidence="7">
        <location>
            <begin position="801"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="79"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="184"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="255"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="79"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="98"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="206"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ84780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="950" mass="105180" checksum="D22B7A08C103728F" modified="2010-06-15" version="1">MIRSFQVKASRPLRRVAPQRVRILVIDFSIRETLPRIVYKPRHRSSAIATTTAAAQRMMQEPPVGGSAGSSLGQFSLSPLSFSGSQSSPSSAKKQKTFHTPGSANPKQTLLKPHQSLLNTLKKSQSPFSPQGARGKTGKNGSILNFFKPASEGKDAGGGNGLNGKEPVEVGAKNDSLFIKHGGYNLDVSDPEDEGGDDSWSPESKIVSRPDFIDELSEVEKDSPTGLGAQEVLGDQESNPDGTLELEEGVVFKEGTGTKECTPPPPSLQGRTPVHATLPETPPPKPNISADMVFGPGGLKLDPGRSMLFSRKGSTPSPFRGLRKRPWADVNDLDAPDDRSYVKSADDLAWKLTLEKAIAEEKASYVPEDKRGQFIKEPSTEIGGFPPNGSQIGNSVLVDDFENIEDFEEVGFEENEDRHLFDPISGAIEQGFQDLDDLSPEEIQMIMEPDRTGSRDISRDEEILHCPMCAAEISHLSERDANAHVNGCLDGKPVPLPFPKPSVRSPVVSPFFSKGPAHISKIPESPSAFTKIMSRNIESQAWASAAKSEMEPRGKWGAQKTCPFFKMLFGGSITVDAFKYGSIPGCQAYFLSHFHSDHYVGLTSKWDHGPIWCSRATANLVRMKLRVDPKYVQEFPFEEWTDVVDGIRVRGLDANHCPGSMLFLFEQDCVGRLGKQRILHCGDFRASAKHLNHPLLRPGKDGVKGQKLDSVYLDTTYLNPKYAFPSQKSVIKACEELCVGLNKESIDGQGGTFAKTATLMNRMGKFGSSSLLWGPIVSEKRKSELQKLFAPKSSHHKIRSKFASVHMTPLMKINPETLRDYLNKFNPHFSRIVGLRPSGWSYRPPSNRLTDSPSVNNVLHSASWKGEYRLSELLPQHGSTRESKCYSVPYSEHSSFRELTMFCCALNIRKIIPTVNIGSEASRDKMNRWFERWEAEKRRSGLFKIEDEEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJU1</accession>
    <name>D5GJU1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00009193001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430333">
        <property type="protein sequence ID" value="CAZ84784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJU1"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009193001"/>
    <dbReference type="HOGENOM" id="CLU_2724050_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJU1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ84784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="72" mass="7816" checksum="EA2A1EE9A581A06C" modified="2010-06-15" version="1">MQPPLPPLLLSTKSVSFRDKGAKKAVCKSVRLRRGTNECIDSEGYGARGSVTIGPATVRVDYTQPSIRVLEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>D5GK15</accession>
    <name>D5GK15_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Tubulin alpha chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">GSTUM_00009314001</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13 15">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4 15">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430335">
        <property type="protein sequence ID" value="CAZ84858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840667.1">
        <property type="nucleotide sequence ID" value="XM_002840621.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GK15"/>
    <dbReference type="FunCoup" id="D5GK15">
        <property type="interactions" value="1104"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GK15"/>
    <dbReference type="GeneID" id="9186895"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009314001"/>
    <dbReference type="eggNOG" id="KOG1376">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015718_1_1_1"/>
    <dbReference type="InParanoid" id="D5GK15"/>
    <dbReference type="OMA" id="YMASCIL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02186">
        <property type="entry name" value="alpha_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000005">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000001">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000008">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002452">
        <property type="entry name" value="Alpha_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01162">
        <property type="entry name" value="ALPHATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11 15" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 15" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="16">
        <location>
            <begin position="49"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="17">
        <location>
            <begin position="248"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAZ84858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="449" mass="50229" checksum="DE5FFEF2CC1AE575" modified="2010-06-15" version="1">MREVISLNVGQAGVQIANSCWELYCLEHGIQPDGYLTEERQKGPQDTGFSTFFSETGQGKYVPRTIYVDLEPNVVDEVRTGTYRSLFHPEQMITGKEDASNNYARGHYTVGKELIDQVLDKIRRVADNCSGLQGFLVFHSFGGGTGSGFGALLMERLSVDYGKKCKLEFSVYPAPQVSTSVVEPYNSILTTHTTLEHSDCSFMVDNEAIYDICRRNLDIERPNYENLNRLIAQVVSSITASLRFDGSLNVDLNEFQTNLVPYPRIHFPLVSYAPVISAARASHEANSVQEISNSCFEPHNQMVKCDPRNGKYMATCLLYRGDVVPKEVHAAVASIKTKRTIQFVDWCPTGFKIGICYQPPQLVPNGDLAKVNRAVCMLSNTTAIAEAWSALSNKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALERDYEEVATDSLGEDAEEPEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5GK91</accession>
    <name>D5GK91_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ESCRT-II complex subunit VPS25</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00009429001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the VPS25 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430337">
        <property type="protein sequence ID" value="CAZ84934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840743.1">
        <property type="nucleotide sequence ID" value="XM_002840697.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GK91"/>
    <dbReference type="FunCoup" id="D5GK91">
        <property type="interactions" value="792"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GK91"/>
    <dbReference type="GeneID" id="9184863"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009429001"/>
    <dbReference type="eggNOG" id="KOG4068">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087657_0_1_1"/>
    <dbReference type="InParanoid" id="D5GK91"/>
    <dbReference type="OMA" id="TRCLIMW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000814">
        <property type="term" value="C:ESCRT II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000141">
        <property type="entry name" value="vacuolar protein-sorting-associated protein 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.570">
        <property type="entry name" value="Winged helix' DNA-binding domain. Chain C. Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008570">
        <property type="entry name" value="ESCRT-II_cplx_Vps25-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014041">
        <property type="entry name" value="ESCRT-II_cplx_Vps25-sub_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13149">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VPS25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13149:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN-SORTING-ASSOCIATED PROTEIN 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05871">
        <property type="entry name" value="ESCRT-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00030094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ84934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="197" mass="23138" checksum="E3EE3CED738354CD" modified="2010-06-15" version="1">MTTTATMFTKPKPPNLEYPELYEFPAFFTRYHSSSSPQNTQPNDTTWAHQRSIWAAWVLSYCRAHKIWKLQLSDALETELFYNRNLNRRFKQRDAAELLEYMVTEGDVEWEGVGKATAIVYWRKPEEWANVIYEWIDSTGQKGSVLTLYEISEGDLTRNQEFHTIDPYVLRKALDFLVKRSLAQVFGANEEMGVKFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKC7</accession>
    <name>D5GKC7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00009479001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430337">
        <property type="protein sequence ID" value="CAZ84970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840779.1">
        <property type="nucleotide sequence ID" value="XM_002840733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKC7"/>
    <dbReference type="STRING" id="656061.D5GKC7"/>
    <dbReference type="GeneID" id="9181990"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009479001"/>
    <dbReference type="eggNOG" id="KOG1911">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_485879_0_0_1"/>
    <dbReference type="InParanoid" id="D5GKC7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="561" mass="62867" checksum="44043FF514247AA2" modified="2010-06-15" version="1">MNLSTINRINKKARNEREPDLNSLDLFEASNPVPFSKKTSLETSTLSPKDLDSLRITAQSTEDRGQVLPRGPSGISRREKEVGQFHDGDLTMSPVHEDRITSIPTADIEQGLGRNSLRRNSNKKLFECEMKIQRGGELESLGVVQFLGFSAQFLDYLDRLGVRELCVTKLLADSHVAGAVITEIGYPQEFADITIHNADKAQPLIITLAKFHGLGIIREAAFTIAVFQPSSDKITTSFKAPLQQQKTPLRAVAYSDWRIPEPSRELVSAERREIGGHVTQYLHTLGERLLSLSFETLIAPTESNPEGVRNFVIYCPDDRFYEKLEMESNLKTLGLKALDSEELLAYLNRGKKMIILVHYTVQFSLHLLPNLKLLQAREHLFFFFGTRFVDNGGTMTLDARPIRFWSFGTLMLATPNFALNNPPMLKRLLDYQAAKAGSTTLCMASTMVTEVEKRITSKPGAFENPQAILEAVYRMQDSAGDTLLVSSREDVNELELIADAFAHHQAVNCALYRRFIVVHAKDEAPPSLGRRYGCVEFHDPDTLFEVVMKGVVPPTRTKPSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKK1</accession>
    <name>D5GKK1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00009602001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430340">
        <property type="protein sequence ID" value="CAZ85044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840853.1">
        <property type="nucleotide sequence ID" value="XM_002840807.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKK1"/>
    <dbReference type="STRING" id="656061.D5GKK1"/>
    <dbReference type="GeneID" id="9183354"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009602001"/>
    <dbReference type="eggNOG" id="ENOG502RYZ7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007073_0_1_1"/>
    <dbReference type="InParanoid" id="D5GKK1"/>
    <dbReference type="OMA" id="CTKNGHQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47783:SF1">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47783">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="4">
        <location>
            <begin position="40"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="76"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="254"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="889"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="76"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="167"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="929"/>
            <end position="938"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ85044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1057" mass="115068" checksum="7E9690FB3A2867CE" modified="2010-06-15" version="1">MATAVVAPQPAHVMQPFTRPIRFVANDGQPHAKRRRISAACKTCRKRKTRCSGEKPICETCRQNGHSCLGYSDPTNSAPVSSAHQTQLQQSQARSESTPTVVTTLTDKPLKEPLFSPTPSSPHDRPRKPSVQQPLPSEQRELRGTESGVKSQKRKSTGYHDAAGRGYEYDMDSRRADGGSGGELTDEGPSPVLRYPSPPSQRLAHRMPYFRYFGPTAIVPGYKQMVVEVHDRNNIPRHSSLSMSANSSVTSPASTFVSLGNPNSTPTVSSGTNSVPMEDIPFYDPSDPAPNNPLIAHLVETFFSHLGCNFPFLRREVFTKDVEEKRVEGILVDAVCAVAARFSSNSLLQSLVRNGERVPPSEYGTAFAIRAKSHVVDTFACPNLATVQACILLAYVEFGSNRDSGLWMFLGIAIRMAQDLGLQKLEGTRGGAEGGSEARRSMGCYDDGVAEGREEYVDNENVREAKARERERTDTFWAVFFLDRVISSGTGRPVTIRDKEIEIDFPPIEEVDPEHGYPPPFPALIRIIQLYGRITDLLNSIKDVHTVTPDTLNSLSGMEADLTKIYQRLSPNLHFGVANFQHYASTNQGAVFLLLHFWFHTLIVLLHRPTLLIAFGGSIQQLFSNSRELSMSSAKTIADILAFAELLDCKAVNGNPFTSQPIYIAACAFLQESAAHSSSNPTSRSSSPPPSGSLPGMIPGFGSKIMEEGTLVQKISTKHALLAQAAHQNYQKCYKALKAMETYWEGVKYILTVMDQKAKGIVDPLLYTVEEMESVEVHRWGAGVWNRFRGNLVPGVEQANGAVHEDIIKLEGGLSQSGLMPPGGQLGLGVNRIPEHVSSPHMNGIHGNPSQAIGWSLTGTTNSPNSNLTFMFPASGEAGVYTIQHQQYNGNASSPHANHHGGIQQAQDPRNGLMTSGIHPPLPHGFVGATPTSTTSLAASPQSKYSVGASAPAASDAEMLLGLSVDQQRQQQKAAGVHHSPMDGLIMGGDIYNNEETGSAMGAFNAYNIMIESEDINMNSMGANQLLPLLEFVPYDSYQYGAEAGEGIVNHHHHQHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKY5</accession>
    <name>D5GKY5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00009835001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Rab GDI family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430342">
        <property type="protein sequence ID" value="CAZ85178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840987.1">
        <property type="nucleotide sequence ID" value="XM_002840941.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKY5"/>
    <dbReference type="STRING" id="656061.D5GKY5"/>
    <dbReference type="GeneID" id="9185772"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009835001"/>
    <dbReference type="eggNOG" id="KOG4405">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021695_3_1_1"/>
    <dbReference type="InParanoid" id="D5GKY5"/>
    <dbReference type="OMA" id="WRSINIV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005968">
        <property type="term" value="C:Rab-protein geranylgeranyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005092">
        <property type="term" value="F:GDP-dissociation inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.405.10">
        <property type="entry name" value="Guanine Nucleotide Dissociation Inhibitor, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.519.10">
        <property type="entry name" value="Guanine Nucleotide Dissociation Inhibitor, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018203">
        <property type="entry name" value="GDP_dissociation_inhibitor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11787:SF4">
        <property type="entry name" value="CHM, RAB ESCORT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11787">
        <property type="entry name" value="RAB GDP-DISSOCIATION INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00996">
        <property type="entry name" value="GDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00891">
        <property type="entry name" value="RABGDIREP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="572" mass="61133" checksum="FA28A42A7823436A" modified="2010-06-15" version="1">MNDFSDIPPLPLADWDVLISGTGVKQSLLAVALCRAGKTVLQLDKNSHYGGSAAAFYSLSEADAWAARVNSGDEVGITNAVVVSNVGEGVGGGNGRGYTLSLAPHLIYWHSSVLEMLRRADLTDGFSWYAVGSWWVCLTDEEVAVPAVATGTGGVVGLAEIGVKAAKLASMTGKKRAGWNKGRKKKPAAKTALEGGEAAVGGMTAQGNGESPLRKVDNFREVPCTYEDVAWSKDLTDKDRGQLGEFLRFVVKYNDPTDQKFTQIFEENRQTSLQTFLSTTFPIPPFITTSISSLTLFPSAPSDITLEQALPRLAAHFTSLGKIPDVRSSAALTIAYGGSAELCQVLSRAAAIAGGINVLGRGVKSLCSNETGDGNRKLEAHLESGETVYADWVIGEASDLAKEVKTSGGKSVSRGIYVIETEISWLFEQKYKDENITPAAAVAVVPGDDERAPVYVIARSGVTGECPRGQCLFHAVTESSYEDMNAAIDKILQTCSPLSGVLYSLQYTVAAPSPEIGAEDGAVILDPLTQDIALQGSVLEECLKIYEEIMGTREGFMDVSEEVRRMKEDMEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>D5GL82</accession>
    <name>D5GL82_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 14">DNA replication licensing factor MCM6</fullName>
            <ecNumber evidence="3 14">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">GSTUM_00010054001</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">Component of the MCM2-7 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the MCM family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3 14"/>
    <dbReference type="EMBL" id="FN430346">
        <property type="protein sequence ID" value="CAZ85275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841084.1">
        <property type="nucleotide sequence ID" value="XM_002841038.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GL82"/>
    <dbReference type="FunCoup" id="D5GL82">
        <property type="interactions" value="1005"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GL82"/>
    <dbReference type="GeneID" id="9182069"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010054001"/>
    <dbReference type="eggNOG" id="KOG0480">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000995_3_1_1"/>
    <dbReference type="InParanoid" id="D5GL82"/>
    <dbReference type="OMA" id="RHQQTDK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071162">
        <property type="term" value="C:CMG complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097373">
        <property type="term" value="C:MCM core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005656">
        <property type="term" value="C:nuclear pre-replicative complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033679">
        <property type="term" value="F:3'-5' DNA/RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990518">
        <property type="term" value="F:single-stranded 3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902969">
        <property type="term" value="P:mitotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006267">
        <property type="term" value="P:pre-replicative complex assembly involved in nuclear cell cycle DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006279">
        <property type="term" value="P:premeiotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17757">
        <property type="entry name" value="MCM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.870:FF:000002">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.10:FF:000003">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000115">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.870">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.10">
        <property type="entry name" value="mini-chromosome maintenance (MCM) complex, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008049">
        <property type="entry name" value="MCM6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041024">
        <property type="entry name" value="Mcm6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF43">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18263">
        <property type="entry name" value="WHD_MCM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01662">
        <property type="entry name" value="MCMPROTEIN6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 14" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 14" id="KW-0235">DNA replication</keyword>
    <keyword evidence="9 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 14" id="KW-0347">Helicase</keyword>
    <keyword evidence="6 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="16">
        <location>
            <begin position="410"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="727"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="44"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="727"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="755"/>
            <end position="766"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00073495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU368064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAZ85275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="888" mass="98414" checksum="3A182133BA7D4C49" modified="2010-06-15" version="1">MSSLVDAVLSSDLPEPAPRPRPRGNPAPSSVRFSSSAPGPPDGTSDDGQMDVDPDDEVVGARGAVNRTNRPRTDLSQVPKVTDEAGDQIMNAFIGFLENFVEDPDNPPPPPSSAAAAAPPTTDKYYIAAIHSMAVTESSTLFVDYAHLTYWSNTHVKGFVEAITERHYRFLPFLKRGLETCIRRYEPEYWGKTGKGGQSENVRSYRLPVTKIVSRFTPSHRSIFCYSVNHTTDKIFSLGIFNLPLTTRIRSLRTLQIGTLTSISATVTRTSEVRPELLLATFTCEACRTEIPGIEQTFRYTEPTQCPNLTCGNRVSWRLEIKQSSFVDWQKVRVQENSGEIPTGSMPRTLDIILRGEIVERAKAGEKCIFTGTLIVVPDVSIAEEEEDFLNSLTQAEIAELKSMVHSDHIYSRLVNSIAPTVYGHEIIKKGILLQLMGGVHKVTPEGMSLRGDVNICIVGDPSTSKSQFLKYVCSFLPRAVYTSGKASSAAGLTAAVVKDEETGEFTIEAGALMLADNGICAIDEFDKMDIADQVAIHEAMEQQTISIAKAGIQATLNARTSILAAANPVGGRYNRKATLRSNINMSAPIMSRFDLFFVVLDECNEAIDTHLARHIVGLHRNRDAAITPEFTTEQLQRYIKFARTFRPVFTEEARTLLVQKYKELRADDAQGGVGRNSYRITVRQLESLIRLSEAIAKANCVEDVTEGFVNEAFGLLRQSIIHVEKEDVEIDDDDEADEEMQEDIDQESREPTAAVSTPGMSSSQPPREKTKITYDRYMQILNLIVRRINDDEESTGQGVEEEELKTWYLEMREEEINTEEELEEEKMLIGKVLRRMVRDNILMQIRGEGLVGEAAEGSSAEGGKTVFVLHPNVALEELGATTTPAPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GL90</accession>
    <name>D5GL90_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00010075001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430347">
        <property type="protein sequence ID" value="CAZ85283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841092.1">
        <property type="nucleotide sequence ID" value="XM_002841046.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GL90"/>
    <dbReference type="GeneID" id="9182077"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010075001"/>
    <dbReference type="eggNOG" id="ENOG502RQ2V">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035126_0_0_1"/>
    <dbReference type="InParanoid" id="D5GL90"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ85283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="398" mass="44561" checksum="4680B198F7CE80E3" modified="2010-06-15" version="1">MTTLKYFNPPAYLTDLTKENASKWSKDYISNWMDQSASTAEVTQFYNATTHATEGEINTKIITWTAFPNTVKHNTPEKERWRVADSTRDNQDEYCEWSVLRNGKGEIKKVVFTCEGPEYWEALATFQPDTLLRLYKENNPEYASQIKSEDLYINKSLRYNRKNKWNNNTTTGSIMHLIQGANTLQAEIQLGADATVLRVDSQGNPIHDYDRLIRCSQYGGIYRNSDPTIGGNVNDLAWQGPLLTLADPVGLYIDSFDGSQFEAPDEEDVQKFWKFTRGTPADSSRGRPAHWVRAVFEVPKSYGYNVSAIKDANGNKITWGSQLADAIQIRLTGQAAEIGKHLGQKRRCKSSGLEAAAATTEVASMSAAANIGEDDTPEHLKGTRAHGLDIDAQGYVCY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLB3</accession>
    <name>D5GLB3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00010118001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 15 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430348">
        <property type="protein sequence ID" value="CAZ85306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841115.1">
        <property type="nucleotide sequence ID" value="XM_002841069.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLB3"/>
    <dbReference type="FunCoup" id="D5GLB3">
        <property type="interactions" value="39"/>
    </dbReference>
    <dbReference type="GeneID" id="9182100"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010118001"/>
    <dbReference type="eggNOG" id="KOG4472">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024327_2_1_1"/>
    <dbReference type="InParanoid" id="D5GLB3"/>
    <dbReference type="OMA" id="TIDEISW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000051">
        <property type="entry name" value="Alpha-1,2-mannosyltransferase (Ktr4)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002685">
        <property type="entry name" value="Glyco_trans_15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121">
        <property type="entry name" value="ALPHA-1,2 MANNOSYLTRANSFERASE KTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121:SF2">
        <property type="entry name" value="MANNOSYLTRANSFERASE KTR5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01793">
        <property type="entry name" value="Glyco_transf_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ85306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="452" mass="52407" checksum="DB8DEB99AC6B5047" modified="2010-06-15" version="1">MGLVSQLVRRIPSKAAIPSLEDISKGRLPSRLSFLRRRIRLKGNSSISVPLGVVLLFPMTVVLLIMLLFSRHPDNRTSILIPSGSPPAIRRISEAYDKPFVVGCLEPVTDTPRVNAAFVVLARNSELEGVIQSVKSIERHFNRWYNYPYVFLNDEEFNETFKSTIINYTSGTVEFGSIPKTMWGFPDWVDKDEATESIAKQGDAAIMYGGMASYHHMCRFYSGFFYKHELLAKYEWYWRLEPEIKYFCDITYDPFLHMQRQNKTYGFTIAVKELKETVPNIFRYASAYKRNNNLPTTEAYNMCHFWSNFEIARLDFFRSEKYEKFFETMDRSGGFWTERWGDAPIHSLAAGVLLEPYDIHYFRDFGYRHTTIQHCPANAPARQLPRPKWLEKTTTDPKKAKEEDDYWENWDSEKENGVGCRCRCDTDIVDVEGKQGSCLSEWVNVAGGWISA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLF5</accession>
    <name>D5GLF5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP-dependent RNA helicase ROK1</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">ATP-dependent RNA helicase rok1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">GSTUM_00010172001</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">ATP-dependent RNA helicase involved in 40S ribosomal subunit biogenesis. Required for the processing and cleavage of 35S pre-rRNA at sites A0, A1, and A2, leading to mature 18S rRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with the U3 snoRNA and is associated with the 90S and 40S pre-ribosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the DEAD box helicase family. DDX52/ROK1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="FN430348">
        <property type="protein sequence ID" value="CAZ85348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841157.1">
        <property type="nucleotide sequence ID" value="XM_002841111.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLF5"/>
    <dbReference type="FunCoup" id="D5GLF5">
        <property type="interactions" value="958"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GLF5"/>
    <dbReference type="GeneID" id="9186092"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010172001"/>
    <dbReference type="eggNOG" id="KOG0344">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_1_4_1"/>
    <dbReference type="InParanoid" id="D5GLF5"/>
    <dbReference type="OMA" id="EMAHSIM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17957">
        <property type="entry name" value="DEADc_DDX52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044764">
        <property type="entry name" value="DDX52/Rok1_DEADc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF15">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="59"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="264"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="442"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="442"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAZ85348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="515" mass="55716" checksum="C71B02EBE1F84761" modified="2010-06-15" version="1">MEPTNANEEEGKKKKKKKEALMIQPLTSFTHLHKLSPRLHRNIRAQGYRVPTEVQMGAMPVISRGENLICCAPTGSGKTVAFMVPVLDWILAQGKGDEGGGEGKRKKVRVVVVAPTKELASQIVNEGKKLALGTGVRVALLKKGGEGVKSEVVVSTPAVLLHSLEASTSCSPSPGEGDEMEEKKTDWSSVSRLILDEADILLDPLFRAQTLGIWTQLQSPHLRTSLFSATISSSTETLCQQHIPPSSSPTIRLIVGLKDTTIQNITQKLTYTATEPGKLLALRQLFTTSFHPPALIFVQTIPRARALVAEILYDLPTPGRIAVLHAELSDVARAEVMGRFLAGEIWVLVTTDLLARGMDFRGVRCVINYDIPTSVAGYIHRVGRMGRSGGVQGGLAITYFTEGDVRFVKGIAGVIAKAQGGGEGGKGEGKWLVNALPKVGKSDKERLKKRGVEARSKAADGKEGRISTKSGYERRRENRKRGAVEGSKRRKVQAWGGGDSDGEEGEEFMGITDLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLG3</accession>
    <name>D5GLG3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00010181001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430348">
        <property type="protein sequence ID" value="CAZ85356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841165.1">
        <property type="nucleotide sequence ID" value="XM_002841119.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLG3"/>
    <dbReference type="GeneID" id="9186100"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010181001"/>
    <dbReference type="HOGENOM" id="CLU_2724052_0_0_1"/>
    <dbReference type="InParanoid" id="D5GLG3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="42"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="72" mass="8069" checksum="EA232AEF22356360" modified="2010-06-15" version="1">MFYLLYIFLAAFFLLTFNSGAFFVFHLPSIVRGFLSLLSIGSTGTTASSSSTRGRQRRPEGLERGSWRQGWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLL3</accession>
    <name>D5GLL3_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST2</fullName>
            <shortName evidence="8">Oligosaccharyl transferase subunit OST2</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00010257001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the oligosaccharyltransferase (OST) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Endoplasmic reticulum membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the DAD/OST2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="FN430349">
        <property type="protein sequence ID" value="CAZ85406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841215.1">
        <property type="nucleotide sequence ID" value="XM_002841169.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLL3"/>
    <dbReference type="FunCoup" id="D5GLL3">
        <property type="interactions" value="673"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GLL3"/>
    <dbReference type="GeneID" id="9182709"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010257001"/>
    <dbReference type="eggNOG" id="KOG1746">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111220_0_0_1"/>
    <dbReference type="InParanoid" id="D5GLL3"/>
    <dbReference type="OMA" id="HIILHIV"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003038">
        <property type="entry name" value="DAD/Ost2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10705">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT DAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10705:SF0">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT DAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02109">
        <property type="entry name" value="DAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005588">
        <property type="entry name" value="DAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="64"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="132"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="8"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ85406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="151" mass="16055" checksum="A086B07CB5844481" modified="2010-06-15" version="1">MPTKKNLSSTSSTSAPTAPASAALSSSSPAPKSHTSHNALSDPLSILGDIFSNYSQSTPQRTKLIDAFLGFLVVMGVLQFVYCVVAGNYPFNAFLAGFSATVGQFVLTASLRIQSNPANKSEFSSVSPERAFADYVFCSVILHLFCTNFIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLL8</accession>
    <name>D5GLL8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glutamate--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Glutamyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">GSTUM_00010264001</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>tRNA(Glu) + L-glutamate + ATP = L-glutamyl-tRNA(Glu) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9663"/>
            <dbReference type="Rhea" id="RHEA-COMP:9680"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.17" evidence="3"/>
    <dbReference type="EMBL" id="FN430349">
        <property type="protein sequence ID" value="CAZ85411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841220.1">
        <property type="nucleotide sequence ID" value="XM_002841174.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLL8"/>
    <dbReference type="FunCoup" id="D5GLL8">
        <property type="interactions" value="749"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GLL8"/>
    <dbReference type="GeneID" id="9182714"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010264001"/>
    <dbReference type="eggNOG" id="KOG1147">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001882_1_2_1"/>
    <dbReference type="InParanoid" id="D5GLL8"/>
    <dbReference type="OMA" id="CPVVDSH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017102">
        <property type="term" value="C:methionyl glutamyl tRNA synthetase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004818">
        <property type="term" value="F:glutamate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006424">
        <property type="term" value="P:glutamyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00807">
        <property type="entry name" value="GlnRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10306">
        <property type="entry name" value="GST_C_GluRS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000070">
        <property type="entry name" value="Bifunctional glutamate/proline--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1160.10:FF:000001">
        <property type="entry name" value="Glutamine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.800.10:FF:000001">
        <property type="entry name" value="Glutamine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.240.10:FF:000004">
        <property type="entry name" value="Glutamyl-tRNA synthetase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1160.10">
        <property type="entry name" value="Glutamyl-trna Synthetase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.800.10">
        <property type="entry name" value="Glutamyl-tRNA Synthetase, Domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.240.10">
        <property type="entry name" value="Ribosomal Protein L25, Chain P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02076">
        <property type="entry name" value="Glu_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050132">
        <property type="entry name" value="Gln/Glu-tRNA_Ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004526">
        <property type="entry name" value="Glu-tRNA-synth_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020059">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_codon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020061">
        <property type="entry name" value="Glu_tRNA_lig_a-bdl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020056">
        <property type="entry name" value="Rbsml_bL25/Gln-tRNA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011035">
        <property type="entry name" value="Ribosomal_bL25/Gln-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049437">
        <property type="entry name" value="tRNA-synt_1c_C2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00463">
        <property type="entry name" value="gltX_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43097:SF5">
        <property type="entry name" value="GLUTAMATE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43097">
        <property type="entry name" value="GLUTAMINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03950">
        <property type="entry name" value="tRNA-synt_1c_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20974">
        <property type="entry name" value="tRNA-synt_1c_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50715">
        <property type="entry name" value="Ribosomal protein L25-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="8 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9 13" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST C-terminal" evidence="14">
        <location>
            <begin position="58"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAZ85411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="721" mass="81652" checksum="78422D87A4795B1E" modified="2010-06-15" version="1">MAPFDLVVAQKASPGLIYPALIASSIVNAGDNIRPVATITYKDVDLLDKKDEASAVGVQFDGEEIIYGLEGVLHRILKSYSSALRSRAESTAEDEWIKFSTTRLSGGDFKATSAALEELNDHLTLRTFLVGYTLTIADIAVWGVLNGNGQAISSIKRNHLPNLVRWFKYIGSMERVTISVNSFKAEANTKKKAKSKETNYDIGLRDTEKGVVTRFPPEPSGYLHIGHAKAAMLNQYFAQHYNGTMIVRFDDTNPTKEKQEFQDSILQDLELLGIKGDKITYTSDHFDALYDFAIKMIKRGKAYCDDTPQEQMRAERMNGIASKNRDRSVEENMEVFGEMARATEVGTRNCLRAKISVDNPNKAMRDPVIYRCNPSVHHRTGERYKVYPTYDFACPLVDSIEGVTHALRTIEYRDRNPQYWWMLEALGMRKVHVWDFARLNFIKTLLSKRKLTWFVEQGLVWGWDDPRFPTVRGIRRRGMTVEALRQFILAQGPSKNVVNLDWTIIWAGNKKVIDPIAPRHTAIIEKSHIRATIAGAPSPAVCEDKPKHKKNPAIGTKKTYFGSTILLDQADARSFRVNEEITLMDWGNAIVTSIERASPESDVTSLTLKLHLEGDFKKTEKKVTWLAAEQSLVPVELVDFDYLITKDKLEEEDDVANFVNKETEFREDAIADENVRDVRVDDIIQFERKGYYRCDRAAAEGVKAVFFLIPTGKGEKPAEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLN8</accession>
    <name>D5GLN8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00010291001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430350">
        <property type="protein sequence ID" value="CAZ85431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841240.1">
        <property type="nucleotide sequence ID" value="XM_002841194.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLN8"/>
    <dbReference type="GeneID" id="9182734"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010291001"/>
    <dbReference type="HOGENOM" id="CLU_578955_0_0_1"/>
    <dbReference type="InParanoid" id="D5GLN8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ85431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="472" mass="53581" checksum="0FF7F8D95FE22572" modified="2010-06-15" version="1">MVCAELGRAIASIRPTLIYPSTASLTQRRRHSSKSDSFSLTILDWRFKTHYDPYYSHEEDWETDHSAYNKTVSTLDSFFDALNPNSITSLDLDFDIRYSRFSGKDLGNGPLALRQSRSIRALRLARMRCDNFKLWRMFTEMGYAGFSPQSLKLINLFMDNTSTSQNFTGWSFAPGFLHNMRDLEFTKFTGMFDLAVAIIQIKNVNSQLERSLSRGGQQSKREKSARIDSLSLSYEAKQADSSTTELLLAPLASLGSSLTYLKLQDTDFTNPRPGSVHYSRSTVLHHSPLESLTKLKTLILAGDVSVFLLSDRLLSIIRCMRQSITYVDICLSTRDLADALLLRAFAQSENLQTLILRAWDKLVTLEPATAPDFTHGQVIPWIEYPPLLTNRRFWQMSLWPVCPKVTVEGTIGFLAKKRGGLKKFGFSRAFCRDDERGRWGEVAEVMERMGGKFLPRTKKAGRAWDEGLPALE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5GM23</accession>
    <name>D5GM23_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RAD52 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00010491001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RAD52 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430351">
        <property type="protein sequence ID" value="CAZ85566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841375.1">
        <property type="nucleotide sequence ID" value="XM_002841329.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GM23"/>
    <dbReference type="STRING" id="656061.D5GM23"/>
    <dbReference type="GeneID" id="9187346"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010491001"/>
    <dbReference type="eggNOG" id="KOG4141">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011431_4_0_1"/>
    <dbReference type="InParanoid" id="D5GM23"/>
    <dbReference type="OMA" id="NLHRHAD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045002">
        <property type="term" value="P:double-strand break repair via single-strand annealing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006312">
        <property type="term" value="P:mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.80:FF:000001">
        <property type="entry name" value="DNA repair protein RAD52 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.80">
        <property type="entry name" value="DNA repair protein Rad52/59/22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041247">
        <property type="entry name" value="Rad52_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007232">
        <property type="entry name" value="Rad52_Rad59_Rad22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042525">
        <property type="entry name" value="Rad52_Rad59_Rad22_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12132">
        <property type="entry name" value="DNA REPAIR AND RECOMBINATION PROTEIN RAD52, RAD59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12132:SF1">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD52 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04098">
        <property type="entry name" value="Rad52_Rad22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="230"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="431"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="277"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="293"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="315"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="340"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="365"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="381"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="444"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="487"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="505"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="539"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00077224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ85566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="563" mass="60894" checksum="3BDBBA13C1DCA68E" modified="2010-06-15" version="1">MSSWDTSQRVIPYTKPEQEGLQTQLDGMLGPEYLNYRPASGGSSVAYLRGDVAITLANEIFGFNGWSSEIKDITVDECLESHGKWNVTTSVHLRITLKDGTFREDIGTGESHNQRTKSAAIIASKKQAVTDALKRTFRTFGNALGNCLYDKQHIKGTRRMKAVQPVQEPNYYKPRSIRDRILKQEESLVTPGLPESLSEVPREKIISENDLAEGFDEYGGDEYDYLGAFEDGDDYSEHVPDEDTVEAASSDDVFKRPTPPTPTGAKSMVARAGGNQSSIVPKNPNSVSVPVLQQGQNGQHVRTSNIPAQPPPQQSHPLQTQNRGPQLPPQNPTIGQRPLQKPPNAPQGPRTPTPTGQMGSRVNLMANTSHNIRNAQQPGPKTSPPPHTIPQPAPALPASSLNGLPETPVSAHVGFFTSKVLLDPKNNQKVLQGDNGVIPPDHVFNPHKESTIPRSAGIDHSKSSPVPRKQIQAGGSTGSPFARKNFENPSSELNRQIGQPPLPRGTSSFRQPTPSVGGIKRPADASTASAASCPGRSTLGDSSETRLNTQTSAYGNDPKRVKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>D5GM64</accession>
    <name>D5GM64_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00010543001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430352">
        <property type="protein sequence ID" value="CAZ85607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841416.1">
        <property type="nucleotide sequence ID" value="XM_002841370.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GM64"/>
    <dbReference type="FunCoup" id="D5GM64">
        <property type="interactions" value="529"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GM64"/>
    <dbReference type="GeneID" id="9187391"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010543001"/>
    <dbReference type="eggNOG" id="KOG3655">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013085_2_0_1"/>
    <dbReference type="InParanoid" id="D5GM64"/>
    <dbReference type="OMA" id="FKEPRGA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030864">
        <property type="term" value="C:cortical actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030427">
        <property type="term" value="C:site of polarized growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11281">
        <property type="entry name" value="ADF_drebrin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11962">
        <property type="entry name" value="SH3_Abp1_fungi_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11961">
        <property type="entry name" value="SH3_Abp1_fungi_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000045">
        <property type="entry name" value="Actin binding protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035719">
        <property type="entry name" value="Abp1_fungi_SH3_C1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035718">
        <property type="entry name" value="Abp1_fungi_SH3_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002108">
        <property type="entry name" value="ADF-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10829">
        <property type="entry name" value="CORTACTIN AND DREBRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10829:SF25">
        <property type="entry name" value="DREBRIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00241">
        <property type="entry name" value="Cofilin_ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00499">
        <property type="entry name" value="P67PHOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00102">
        <property type="entry name" value="ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51263">
        <property type="entry name" value="ADF_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="ADF-H" evidence="5">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="621"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="709"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="156"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="300"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="685"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="358"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="418"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="441"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="458"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="529"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="593"/>
            <end position="609"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ85607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="767" mass="80901" checksum="B493E771917A50E6" modified="2010-06-15" version="1">MASLDISHEIGRSYQTLVNSPPANKPSATFAQWAVFTVSTPLVNAFVAASASKASVLKVHSTAEGELEDLIEEFNDGKVQFAFVKVKDANTSLAKFVLIAWCGEGVPERVKGYFTGHLNTVSKVLHGYHVQITARSESDLLPESILQRVADASGAKYSAHGSTSGPPLTAPKPTVSAHKPAHRPTKVSGGPPNPASSRSPYKRDESTDADGWGADAPPVTRSQLEKVSSAYKPTKVDIAVLRAQPTSTTSGPVSGSNPDVVKGGYQPIGKVDIAALRAQGQKQDRPDIVKGTYEPIGKVDIAAIRAQAQPRPSPNSPGPRSNAPAQEEEDHKPLAQRSAVFSQSERLTSLPKPKPAKKFGAGAPAFGTKPPTPAAFGISPTVQPTPPVGAASRDFGSSGGKTPAQLWAEKKARERGTSGASEITPPTISPGYTGQHHPPLGTSSTGRSTGSADKLMTGHSQQRIDSSQSHEEEVQTPAGGIGALRSKFAGAPPMGTPASPKSPQRTGGFHGAEREVPPPPPIDMSSRPLPAPAVAVPPPPRQPRSPTPPTPEAPGSPVRIAMPVARQSDSPPMAKHEPEPQISMPAESLARAVPDERELQEEEPARDRGAVVGVSAGGEGASGKRALVLYDYDAAEENEINLVEGQVVGEIDMVDEDWWAGRNAAGEHGLFPSNYVELMGDTEVAQSPRGAPADSPEPEHQPPQPQAPGSGDTAIALYDYEAAEENELSFPEDAIIEDLEFPDEDWWLGTYQGNRGLFPANHVELRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMB6</accession>
    <name>D5GMB6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00010638001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430354">
        <property type="protein sequence ID" value="CAZ85659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841468.1">
        <property type="nucleotide sequence ID" value="XM_002841422.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMB6"/>
    <dbReference type="FunCoup" id="D5GMB6">
        <property type="interactions" value="140"/>
    </dbReference>
    <dbReference type="GeneID" id="9184459"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010638001"/>
    <dbReference type="eggNOG" id="KOG2533">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_0_1_1"/>
    <dbReference type="InParanoid" id="D5GMB6"/>
    <dbReference type="OMA" id="IYYFTRW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000034">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF91">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="41"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="138"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="200"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="324"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="356"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="380"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="449"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="41"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ85659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="512" mass="56274" checksum="B687FDA5D9CFC8D0" modified="2010-06-15" version="1">MGKESTDSSLGRVQVRQPGSIEGGVDHEFEKKLVRKLDNHIVPMIMLLYLFSFLDRVNIGNARLYGLEEDLGLKGNQFQISVSILFVTYLVFEIPSNLVLKKLTPRRYIAAITTIWGIIATCTGLVNSYGGLLACRLLLGAVEAGLFPGLAVYLTFFYTRKELALRIGYLFVSAALAGACGGLLAYGIGFLDGRSGMKGWRWILIIEGLPTIVLGLVTYFVLADNPEEAGYLTEEEKRFMIARRAREVTQTTSAQEFHWGDVRKCFLDWKCWAFAFAQFGIDTMLYGPSLIFASTPLASILTPPPGFSTFLPTIIKAIGHWSTAQVQALTIPCYALGALTYLIVAHISDKQQRRGMYTVSFACISIVGYGILLSDAPSGAHYFGCFLVAIGLYVSVGLPLAWLPNNCPRYGKRAAASGFQLTVGNASGIMAPFVKSYTKATSRYTKGHAVSLSMVGFAAIIYTFLWHWFSKLNISRERGEENAKIVGLSEEDIAELGDESWVPFAPQFTGGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMU0</accession>
    <name>D5GMU0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00010930001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430359">
        <property type="protein sequence ID" value="CAZ85833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841642.1">
        <property type="nucleotide sequence ID" value="XM_002841596.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMU0"/>
    <dbReference type="FunCoup" id="D5GMU0">
        <property type="interactions" value="26"/>
    </dbReference>
    <dbReference type="GeneID" id="9185169"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010930001"/>
    <dbReference type="eggNOG" id="ENOG502QUA0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020178_1_0_1"/>
    <dbReference type="InParanoid" id="D5GMU0"/>
    <dbReference type="OMA" id="RTHCLEY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022703">
        <property type="entry name" value="DUF3533"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053001">
        <property type="entry name" value="MNNG_permease-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34814">
        <property type="entry name" value="NITROSOGUANIDINE RESISTANCE PROTEIN SNG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34814:SF1">
        <property type="entry name" value="NITROSOGUANIDINE RESISTANCE PROTEIN SNG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12051">
        <property type="entry name" value="DUF3533"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="207"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="321"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="378"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3533" evidence="3">
        <location>
            <begin position="45"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3533" evidence="3">
        <location>
            <begin position="291"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="259"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ85833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="422" mass="46497" checksum="F7F2CA214A7A7E43" modified="2010-06-15" version="1">MGNGSVINWSLYAGLPIPLYGPNPTRLATSTDSPCNAESKSGASLVDFDSPTHETALIGPAVLRAAESQNALRQPHLGYIVKSPDEYPGGETQVRKVVYEQKHWAAISITSNATARLENALRSGDEGFDPDSLAEIVFVEAREESVTRNYLVPRLDDLKGEVVRGFSEVWIPRVVREEGLRRNMARVPLAVNPGFGFRMTNLRPFDVPAAIPAVSVGLLCIPPLALTLPLGMLKRRNNVDGADRLAFIIPRDGNSHAHIFTNDHSPHNKTSPPCPSPNGSSGALRQPSNYNFPIYWMLNWVGMSALGLASENMSMVLGPPWFALWLIFWAITNVATSFYPLVLAPGIYRFGYAWPLHNIVAASRSIVFDVRNEIGRNFGVLFAWVCVSVLLFPVCTLIARRRAERGRMDEVGVRKEGNEVPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMU6</accession>
    <name>D5GMU6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00010937001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430359">
        <property type="protein sequence ID" value="CAZ85839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841648.1">
        <property type="nucleotide sequence ID" value="XM_002841602.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMU6"/>
    <dbReference type="STRING" id="656061.D5GMU6"/>
    <dbReference type="GeneID" id="9182343"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010937001"/>
    <dbReference type="HOGENOM" id="CLU_1157113_0_0_1"/>
    <dbReference type="InParanoid" id="D5GMU6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591:SF24">
        <property type="entry name" value="2-METHOXY-6-POLYPRENYL-1,4-BENZOQUINOL METHYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13489">
        <property type="entry name" value="Methyltransf_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="240" mass="27479" checksum="6EB6F1EC523F116C" modified="2010-06-15" version="1">MSRYSYSEEEEEVEYSPDEEPSSQEEYDDNLSTMSLSDSITDYVYENGRRYHSYRQGSYDSAYGSYWTLGRVLASGQLTSLSITPLCSMLTCFQISSAQIIGNDLSPIQPSYVPPNLRFEIDDCEEDWPFPDNHFDFIHIRNLVGSVRDWDRIYVQAYRTLKPGGWIELKDNFRPFECDNGTLKPDNVLRTWVDNFEKATIIAGINWGTAAAEFRPSLEKAGFKAVTEQIHKVPLGYVPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>D5GN15</accession>
    <name>D5GN15_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Dolichol-phosphate mannosyltransferase subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00011014001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Stabilizer subunit of the dolichol-phosphate mannose (DPM) synthase complex; tethers catalytic subunit to the ER.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the dolichol-phosphate mannose (DPM) synthase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Endoplasmic reticulum membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the DPM3 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430361">
        <property type="protein sequence ID" value="CAZ85890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841699.1">
        <property type="nucleotide sequence ID" value="XM_002841653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GN15"/>
    <dbReference type="STRING" id="656061.D5GN15"/>
    <dbReference type="GeneID" id="9183458"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011014001"/>
    <dbReference type="eggNOG" id="KOG4841">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_150782_1_1_1"/>
    <dbReference type="InParanoid" id="D5GN15"/>
    <dbReference type="OMA" id="DCPEAYT"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033185">
        <property type="term" value="C:dolichol-phosphate-mannose synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013174">
        <property type="entry name" value="DPM3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16433">
        <property type="entry name" value="DOLICHOL-PHOSPHATE MANNOSYLTRANSFERASE SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16433:SF0">
        <property type="entry name" value="DOLICHOL-PHOSPHATE MANNOSYLTRANSFERASE SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08285">
        <property type="entry name" value="DPM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ85890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="92" mass="10424" checksum="BD5005A629EC42E0" modified="2010-06-15" version="1">MTRATETISIALLLSSLYLAAYLRLIPFPDKIQEEIIPVLPFWGLVSFGSYLLFSLGWGIVTFRDTEEAYKELVVEIETAKRELRGKGVDVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>D5GN84</accession>
    <name>D5GN84_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00011154001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430362">
        <property type="protein sequence ID" value="CAZ85977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841786.1">
        <property type="nucleotide sequence ID" value="XM_002841740.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GN84"/>
    <dbReference type="STRING" id="656061.D5GN84"/>
    <dbReference type="GeneID" id="9185903"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011154001"/>
    <dbReference type="HOGENOM" id="CLU_729958_0_0_1"/>
    <dbReference type="InParanoid" id="D5GN84"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="102"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="239"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="257"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="290"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="324"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="356"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="379" mass="39830" checksum="D3DE04F6EDE65D34" modified="2010-06-15" version="1">MADSESPAPTPNSTLKRSNLKTKILEGSRKRSSVSFAEDPTEIASPTPIGKKNTPAPVLGSKVTPILPPGYSANKLVVEAVVMKTPARPAAETESPAKKTKLAILPPKPATAKKASPQPSLVPAQASPQGQGVEEPTLKSKPSLKSDSESEYSTSEDSEESEEEEEEEAKPSSKSSVLKKSTARVKSAKNASPLLTLKASSFTTTNSLKTPPSTYQSSRRSSRASPQLARKTPIIAPATPSTETNSESGSEGDEEIGSASESDFEPAPTTKPATASAKTRPPPQPYRSLSDLEKMPVPDVHDIHSSRTPPNPPPSGTVRKAHDGDDDSSDDSSESGESESSDDEGEVAGKHVINSAKKAAAAPAKKAGFLSNFWGRTER</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>D5GP62</accession>
    <name>D5GP62_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00011723001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0057 (PMP3) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430373">
        <property type="protein sequence ID" value="CAZ86327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842136.1">
        <property type="nucleotide sequence ID" value="XM_002842090.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GP62"/>
    <dbReference type="FunCoup" id="D5GP62">
        <property type="interactions" value="322"/>
    </dbReference>
    <dbReference type="GeneID" id="9186567"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011723001"/>
    <dbReference type="eggNOG" id="KOG1773">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107649_1_0_1"/>
    <dbReference type="InParanoid" id="D5GP62"/>
    <dbReference type="OMA" id="EYVYYER"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000612">
        <property type="entry name" value="PMP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21659">
        <property type="entry name" value="HYDROPHOBIC PROTEIN RCI2 LOW TEMPERATURE AND SALT RESPONSIVE PROTEIN LTI6 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21659:SF42">
        <property type="entry name" value="UPF0057 MEMBRANE PROTEIN ZK632.10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01679">
        <property type="entry name" value="Pmp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01309">
        <property type="entry name" value="UPF0057"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072499" evidence="6">
        <location>
            <begin position="24"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ86327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="93" mass="9947" checksum="0781D8CE7BE31B8F" modified="2010-06-15" version="1">MGVVSAILLILITIIFPPLGVFAVAGCGPDLLINICLTILGYLPGHLHAFYIEYVYYERSEQAVAGRIASERAPGIYSERVQGTVYGATSNSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPH2</accession>
    <name>D5GPH2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00011844001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430375">
        <property type="protein sequence ID" value="CAZ86415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842224.1">
        <property type="nucleotide sequence ID" value="XM_002842178.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPH2"/>
    <dbReference type="STRING" id="656061.D5GPH2"/>
    <dbReference type="GeneID" id="9186655"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011844001"/>
    <dbReference type="HOGENOM" id="CLU_790345_0_0_1"/>
    <dbReference type="InParanoid" id="D5GPH2"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008092">
        <property type="term" value="F:cytoskeletal protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.700">
        <property type="entry name" value="SLA1 homology domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007131">
        <property type="entry name" value="SHD1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03983">
        <property type="entry name" value="SHD1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SLA1 homology" evidence="2">
        <location>
            <begin position="190"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="SLA1 homology" evidence="2">
        <location>
            <begin position="273"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ86415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="351" mass="39036" checksum="6909EB6FEE814626" modified="2010-06-15" version="1">MPSLDFIQAPPHTGCENLADSFCLIEHHELLDENDGFHEIQRNGRLDIGSFESDDENLSSEEDECLLRAVEGDGEEDSSFTLVVRDARPRTHKRMPMREQERDGGGESPGEMDYKHGGKPLLVFRSEGKAGRVASSGSSRGVGGNGEGIEDKEISPKLNSEPEYEPDPETHLAEPNPKSREPSRKPRTAMEYRIWTDKNGQYRQEMILLGCENDHVLLLKRDGVRLNILKSDLSPQDQEFVTQAMEPPITTSLNGLSPNDNTSVIPLYSYSNKPKPDPSKIRSWLDITGRYSVDAQLVGFRKGKIVLHKTDGVKVGVIPNQMAKDDLAFAEKELGMVLTGIAKERRGKAIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPI7</accession>
    <name>D5GPI7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00011859001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the adaptor complexes medium subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430375">
        <property type="protein sequence ID" value="CAZ86430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842239.1">
        <property type="nucleotide sequence ID" value="XM_002842193.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPI7"/>
    <dbReference type="STRING" id="656061.D5GPI7"/>
    <dbReference type="GeneID" id="9186670"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011859001"/>
    <dbReference type="eggNOG" id="KOG2740">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017139_0_0_1"/>
    <dbReference type="InParanoid" id="D5GPI7"/>
    <dbReference type="OMA" id="LNEMCDG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030131">
        <property type="term" value="C:clathrin adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09252">
        <property type="entry name" value="AP-3_Mu3_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14837">
        <property type="entry name" value="AP3_Mu_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1170">
        <property type="entry name" value="Mu homology domain, subdomain B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050431">
        <property type="entry name" value="Adaptor_comp_med_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036168">
        <property type="entry name" value="AP2_Mu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001392">
        <property type="entry name" value="Clathrin_mu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028565">
        <property type="entry name" value="MHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10529">
        <property type="entry name" value="AP COMPLEX SUBUNIT MU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00928">
        <property type="entry name" value="Adap_comp_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005992">
        <property type="entry name" value="Clathrin_mu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49447">
        <property type="entry name" value="Second domain of Mu2 adaptin subunit (ap50) of ap2 adaptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51072">
        <property type="entry name" value="MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MHD" evidence="6">
        <location>
            <begin position="188"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR005992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ86430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="528" mass="56827" checksum="5BD6EA96FB69BB92" modified="2010-06-15" version="1">MSAVEALYVFDSFSPATTPILQHEWRSRPATNAASLYSLYKAQPSPRPSLIYLPTTSPPILLFNIIHNNLLFLSPATSEIEPLLVSEFLHRVVNVLEDYLGSPLLGSKIEANYDIVAQLLNEMCDDGFPFTTEPNALRDVVLPPSLMGKLLGSVTGLPSASIPSSFTPSPTRTLSTIPWRRSNVRHTNNELYVDIIELITATIAPSGRFLSARSSGTIAFNCKLSGIPDLLMTLQAPTNHKQKLGAPPSGGLGFPVFHPCVRLSRWKERPGELSFVPPDGKFVLASYEIDLLPSGNPKSAIQPQLPVSVELKSAIGPQSSEFEARVFISTASLAPTANTLSANPFGPRSSRSPAFGGSSNNPTVEEVSIIFPLPPTVKTLTGTRCSRGEFQHEGRRVTWKIPTTSAFSGTATLRTGVVVKSYGGDESEGEEEAAVDNGEYQDVQTAGTAGVNGGNEEEKAKRERQAKRKMEMAMPRCALVAFSVKGWLASGVKVDSLKVVGGKGLGEGVKPYKGVKYLTKAGDIEVRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPK1</accession>
    <name>D5GPK1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00011881001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430375">
        <property type="protein sequence ID" value="CAZ86444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842253.1">
        <property type="nucleotide sequence ID" value="XM_002842207.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPK1"/>
    <dbReference type="STRING" id="656061.D5GPK1"/>
    <dbReference type="GeneID" id="9186684"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011881001"/>
    <dbReference type="eggNOG" id="KOG1681">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_7_0_1"/>
    <dbReference type="InParanoid" id="D5GPK1"/>
    <dbReference type="OMA" id="VGGGCQL"/>
    <dbReference type="UniPathway" id="UPA00659"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051750">
        <property type="term" value="F:delta(3,5)-delta(2,4)-dienoyl-CoA isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.12.10:FF:000004">
        <property type="entry name" value="Delta3,5-delta2,4-dienoyl-CoA isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000024">
        <property type="entry name" value="Delta3,5-delta2,4-dienoyl-CoA isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="entry name" value="Lyase 2-enoyl-coa Hydratase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045002">
        <property type="entry name" value="Ech1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43149:SF1">
        <property type="entry name" value="DELTA(3,5)-DELTA(2,4)-DIENOYL-COA ISOMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43149">
        <property type="entry name" value="ENOYL-COA HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ86444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="272" mass="29207" checksum="84AE7808006689ED" modified="2010-06-15" version="1">MSQAYNHFNVTFPAPYVAHVEINRPEKLNAFYQEMWLEYAKVFNTLSHSPDVRSIILSGAGSTFTTGLDIQKTNLAFPGSDGARKAWGIRRSVVELQECVSASEKCEKPVICVLHGFCLGLAIDIACATDIRISSADAKLAVKEVDIGLAADVGTLSRLPKVVGSLSWVKDVAFSARMFNPQEALRVGFVSAVESDKAAAVNKAIEMASLIAAKSPVAVQGTKAIVNWSVDRTVADGLAYTQAWNAAALQTEDVGEAVGGTLGKRKTRFEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5GQ03</accession>
    <name>D5GQ03_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00012119001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sirtuin family. Class I subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430380">
        <property type="protein sequence ID" value="CAZ86596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842405.1">
        <property type="nucleotide sequence ID" value="XM_002842359.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GQ03"/>
    <dbReference type="FunCoup" id="D5GQ03">
        <property type="interactions" value="80"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GQ03"/>
    <dbReference type="GeneID" id="9182360"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012119001"/>
    <dbReference type="eggNOG" id="KOG2683">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023643_1_1_1"/>
    <dbReference type="InParanoid" id="D5GQ03"/>
    <dbReference type="OMA" id="RRHYWAR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017136">
        <property type="term" value="F:histone deacetylase activity, NAD-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1600.10">
        <property type="entry name" value="SIR2/SIRT2 'Small Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050134">
        <property type="entry name" value="NAD-dep_sirtuin_deacylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003000">
        <property type="entry name" value="Sirtuin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026591">
        <property type="entry name" value="Sirtuin_cat_small_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026590">
        <property type="entry name" value="Ssirtuin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085:SF10">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02146">
        <property type="entry name" value="SIR2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50305">
        <property type="entry name" value="SIRTUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Deacetylase sirtuin-type" evidence="6">
        <location>
            <begin position="18"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="354"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="146"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ86596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="373" mass="40465" checksum="1298A446BDF7177A" modified="2010-06-15" version="1">MVTPRIPYVLPFPPPPIHPKNATTLSAAIDAVTNFLESRDVVILTGAGISVESGLADYRGEKGTYRLNRTYRPIFYEEFAGNHEARKRYWTRSFLGWPTMEKAQPNRVHRSISILGKLGVLNHVITQNVDSLHHTCHPHLRTTELHGTLQTLICLTCRSPYPRVEFQKTLAELNPKWAEFLHIATEAGMFGNGHRRGQSIKTNPDGDVDILGAPYTKFRYPPCPKCLNSNDIKVLVDEQGSHRPNGGSATNGVLKPSVTFFGESILLEAKTKAEEMVDKCGGILVVGTSLATYSAYRLVKASHDAGKGVGIVSLGGVRGEGLFFSEGSKGRRLRVDFGAGDILGGVVKNFGGEAVEEEESETPLSGGGVGLGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>D5G414</accession>
    <name>D5G414_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">asparagine synthase (glutamine-hydrolyzing)</fullName>
            <ecNumber evidence="2">6.3.5.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">GSTUM_00003903001</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-aspartate + L-glutamine + ATP + H2O = L-asparagine + L-glutamate + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12228"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (L-Gln route): step 1/1.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.4" evidence="2"/>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835136.1">
        <property type="nucleotide sequence ID" value="XM_002835090.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G414"/>
    <dbReference type="FunCoup" id="D5G414">
        <property type="interactions" value="528"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G414"/>
    <dbReference type="GeneID" id="9182384"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003903001"/>
    <dbReference type="eggNOG" id="KOG0571">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014658_2_2_1"/>
    <dbReference type="InParanoid" id="D5G414"/>
    <dbReference type="OMA" id="GIVCAFD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004066">
        <property type="term" value="F:asparagine synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070981">
        <property type="term" value="P:L-asparagine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01991">
        <property type="entry name" value="Asn_synthase_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00712">
        <property type="entry name" value="AsnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000031">
        <property type="entry name" value="Asparagine synthase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000050">
        <property type="entry name" value="Asparagine synthetase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006426">
        <property type="entry name" value="Asn_synth_AEB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001962">
        <property type="entry name" value="Asn_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050795">
        <property type="entry name" value="Asn_Synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033738">
        <property type="entry name" value="AsnB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01536">
        <property type="entry name" value="asn_synth_AEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006949">
        <property type="entry name" value="PRK09431.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11772">
        <property type="entry name" value="ASPARAGINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11772:SF2">
        <property type="entry name" value="ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00733">
        <property type="entry name" value="Asn_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13537">
        <property type="entry name" value="GATase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001589">
        <property type="entry name" value="Asn_synthetase_glu-h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 11" id="KW-0061">Asparagine biosynthesis</keyword>
    <keyword evidence="6 10 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 11" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 10 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="14">
        <location>
            <begin position="2"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="11">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="339"/>
            <end position="340"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for beta-aspartyl-AMP intermediate formation" evidence="13">
        <location>
            <position position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR001589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAZ79257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="541" mass="61026" checksum="F1BA12D6AFDB2B11" modified="2010-06-15" version="1">MCGIFACHKHPDVHKFKPQALQLAKAVRHRGPDWSGNIISNNTILTHERLSIVGIDSGAQPLVSDDGTIFLAVNGEIYNHRILRKQLKPEVTFRTHSDCEVILHLYPNHGINTPKLLDGMFSFVLYDSKNDRTIAARDPIGITSFYQGWSSKTPGAVYFASELKCLHPVCDKIVSFPPGHIYDSLTKKTTRYFEPSWWDPKMVPFHPVDYKLIRTTLEKSVRKRLMAEVPYGVLLSGGLDSSLIASIAAHDSTVTALPELHSFSIGLSGSPDTIAAKRVAKFLGTVHHEFNFTLQEGLDALRDVIYHLESYDVTTIRASTPMYLLSRKIKALGVKMVLSGEGSDEIFGGYLYFVNAPSKDGFHAENIRRVKNLHLSDCLRANKSTAAWGVEARVPFLDKQFLEVSLNVDPAEKLITKERIEKFILRKAFDTSDDPDAKPYLPDDILWRQKEQFSDGVGYGWIDILKDTAEKMVSDEQMKSPKPEWGADVPDSKEGYWYRCMFDEQFPAQCAPTVMRWTPMWTKVTDPSGRAISIHVAKYDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4A1</accession>
    <name>D5G4A1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00004017001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
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    </evidence>
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</entry>
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            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
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                <person name="Anthouard V."/>
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                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat CDC20/Fizzy family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429993">
        <property type="protein sequence ID" value="CAZ79585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835428.1">
        <property type="nucleotide sequence ID" value="XM_002835382.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4Z2"/>
    <dbReference type="FunCoup" id="D5G4Z2">
        <property type="interactions" value="145"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G4Z2"/>
    <dbReference type="GeneID" id="9183067"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000167001"/>
    <dbReference type="eggNOG" id="KOG0305">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014831_3_0_1"/>
    <dbReference type="InParanoid" id="D5G4Z2"/>
    <dbReference type="OMA" id="IPWGPYG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010997">
        <property type="term" value="F:anaphase-promoting complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990757">
        <property type="term" value="F:ubiquitin ligase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905786">
        <property type="term" value="P:positive regulation of anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033010">
        <property type="entry name" value="Cdc20/Fizzy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056150">
        <property type="entry name" value="WD40_CDC20-Fz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918">
        <property type="entry name" value="CELL DIVISION CYCLE 20 CDC20 FIZZY -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918:SF5">
        <property type="entry name" value="MEIOSIS-SPECIFIC APC_C ACTIVATOR PROTEIN AMA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24807">
        <property type="entry name" value="WD40_CDC20-Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="CDC20/Fizzy WD40" evidence="5">
        <location>
            <begin position="214"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="44"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF24807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ79585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="585" mass="63620" checksum="AEE07B91A07B5CA3" modified="2010-06-15" version="1">MSQSSKSSKISPDRFIPSRANIQSFRMSAPAHLLSNNERLLRRSLSSGTTNRSISPNNLSNSPNGRNAGGSPINYRPGNLNRRISAGAVGVFGIGVGAAPRREEPRPRGREVTINVFGNKTSPEVDMERHQRRLSAALGVDRSAKVLSFARENEPTRDGHGRKGGVMGAPGSETVSDVLWEGVLEAEGRDSTPQVSPSRRCLRRAIPTTPFRVLDAPGLRDDYYCSLIAYSPVTHSLVVGLHTDVYSWTETSGARPFEAWSNSHVTALAFSCFQGKRNILAIGRIDGSLSLWNPAEPTPRLERTHDTGIACFAWKPIVSYRPDLGLQPSSGFSSSDGRSQSIVVHSQQICGLAWSGDGEQFATGGNDNVACLFSTADVTRNRSGGVRNHEATPLEGGEKHRWAHGAAVKAIAFCPWQRSLLATGGGSNDRCIHFFHTFSGATLATINVSAQVTSLLWSNSHREIAATLGYANPEHPIRIAVFSWPECRQVVQIPWQEDMRALYAVAYPGGPNDDFPPAGGSRERNKHANRVEGCIVVAASDETVRFHEVWSETRRGVLRWRGVLGGSDILEGLEGIEKDSAETIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5D2</accession>
    <name>D5G5D2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00004276001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835568.1">
        <property type="nucleotide sequence ID" value="XM_002835522.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5D2"/>
    <dbReference type="GeneID" id="9188144"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004276001"/>
    <dbReference type="HOGENOM" id="CLU_1741893_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5D2"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ79725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="150" mass="16108" checksum="10258A92A1E726F0" modified="2010-06-15" version="1">MFGEGYSAEDKGSLKLGRDVEARHASLNHGTAIDSRRAEVDLPEAIKFGKATWGLSSRQLQLKAIGDSTGTGLFWLLTRGDQNAPAEAGPWLVFLAYYFALLPSIGGGSRIRSISLLRRWANGALSPDAGLRISVGLRRLSVLTSLLGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5E6</accession>
    <name>D5G5E6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00004297001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835582.1">
        <property type="nucleotide sequence ID" value="XM_002835536.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5E6"/>
    <dbReference type="FunCoup" id="D5G5E6">
        <property type="interactions" value="140"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G5E6"/>
    <dbReference type="GeneID" id="9188158"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004297001"/>
    <dbReference type="eggNOG" id="KOG4614">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047290_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5E6"/>
    <dbReference type="OMA" id="YFPNFHG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033615">
        <property type="term" value="P:mitochondrial proton-transporting ATP synthase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007849">
        <property type="entry name" value="ATP10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28106">
        <property type="entry name" value="MITOCHONDRIAL ATPASE COMPLEX SUBUNIT ATP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28106:SF1">
        <property type="entry name" value="MITOCHONDRIAL ATPASE COMPLEX SUBUNIT ATP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05176">
        <property type="entry name" value="ATP-synt_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="308"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="329" mass="36843" checksum="F60EEA3B21CE08E7" modified="2010-06-15" version="1">MNTALRLHPLLLRPPPHFSSRSLNARRSSLLHLLLQSSYSTVSTPTPPLPKPNRDHPPPPLNRPIGLPYPPEPLLPSPNISATGKNGFRTLNQIYTDLRDPGRNLAKRRHLTKELAKPYFRDISRLSVTDKGKSFIAPQRLFKSERALWFPNLNGRTLVAAGKDTTGVLRGRVSIVAVFSGTWAERQVELWGLDEVAGMAREAGGEEGVVGRMDINVEENLLRYCIVMMFRGRLMRRMEESRWGRYFIIKEGLGDETRDQMGYHNSKVGYVYLLDEECRIRWAGSGPPMALERAALIRGVKKLVADSKRKPGGVRAGGKEGEGVNMGKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5F2</accession>
    <name>D5G5F2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00004308001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uS13 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835588.1">
        <property type="nucleotide sequence ID" value="XM_002835542.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5F2"/>
    <dbReference type="FunCoup" id="D5G5F2">
        <property type="interactions" value="981"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G5F2"/>
    <dbReference type="GeneID" id="9183731"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004308001"/>
    <dbReference type="eggNOG" id="KOG3311">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103849_0_1_1"/>
    <dbReference type="InParanoid" id="D5G5F2"/>
    <dbReference type="OMA" id="SYKGVRH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.50:FF:000002">
        <property type="entry name" value="40S ribosomal protein S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.910.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.910.10">
        <property type="entry name" value="30s ribosomal protein s13, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01315">
        <property type="entry name" value="Ribosomal_uS13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027437">
        <property type="entry name" value="Rbsml_uS13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001892">
        <property type="entry name" value="Ribosomal_uS13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010979">
        <property type="entry name" value="Ribosomal_uS13-like_H2TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018269">
        <property type="entry name" value="Ribosomal_uS13_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003140">
        <property type="entry name" value="PRK04053.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10871">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S13/40S RIBOSOMAL PROTEIN S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10871:SF3">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00416">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46946">
        <property type="entry name" value="S13-like H2TH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00646">
        <property type="entry name" value="RIBOSOMAL_S13_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50159">
        <property type="entry name" value="RIBOSOMAL_S13_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ79745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="216" mass="24719" checksum="A6829203166D5EB3" modified="2010-06-15" version="1">MPYYAKLFEMVFGAADLIRQLSWGTTLIFHPSSPSLNSTQRHPQILPIAKAALESSYRPPTMSLVSGEKSNFQFILRLLNTNVDGKQKVMYALTKIKGVGRRYSNLVCKKADVDLNKRAGELTTEELERIVTIIQNPTQYKIPTWFLNRQRDIIDGKDSQILANSLDNKLREDLERLKKIRAHRGLRHYWGLRVRGQHSKTTGRRGRTVGVSKKKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5L4</accession>
    <name>D5G5L4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00004398001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835650.1">
        <property type="nucleotide sequence ID" value="XM_002835604.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5L4"/>
    <dbReference type="STRING" id="656061.D5G5L4"/>
    <dbReference type="GeneID" id="9183793"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004398001"/>
    <dbReference type="eggNOG" id="KOG1812">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_348835_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5L4"/>
    <dbReference type="OMA" id="HERERMT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20339">
        <property type="entry name" value="BRcat_RBR_RNF216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20353">
        <property type="entry name" value="Rcat_RBR_RNF216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16630">
        <property type="entry name" value="RING-HC_RBR_RNF216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047545">
        <property type="entry name" value="BRcat_RBR_RNF216"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051628">
        <property type="entry name" value="LUBAC_E3_Ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047546">
        <property type="entry name" value="Rcat_RBR_RNF216"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047544">
        <property type="entry name" value="RING-HC_RBR_RNF216"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22770:SF47">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22770">
        <property type="entry name" value="UBIQUITIN CONJUGATING ENZYME 7 INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26200">
        <property type="entry name" value="Rcat_RNF216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26191">
        <property type="entry name" value="RING-HC_RBR_RNF216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="240"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="92"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="500"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="699"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="97"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="539"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="713"/>
            <end position="723"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ79807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="723" mass="81297" checksum="7F3E9D52445CAA85" modified="2010-06-15" version="1">MEISRDEPIRTERERDGNGKQVERVEEEKKPVASQEDVLQNVLSIFPDACLTHIAKLYREYYLLQGSDITEFIANKLAETDYPKSELVQKAGMKRKRVEEDEKGEKGPDYEANDRPPVVGERYELVRSILKQEFPLMPVRYIEKVMTQNRNYLAKSYSALVKADRSHNPQTNAPYKQLVHPRRTEGQSHELKKGKDWTEISAELEYARGEVRREEMQAQMDADARLAAQINEKEHQESNMLMECGCCFGEYPFDSMTHCNDLHLFCLDCARRNAETEVGNGRHKLSCMDGSGCKALFPRSQMLRFLDGKTLSALAKIEQEDALRIAEIDGLVKCPFCDYGAICAPPEVDKEFKCQNPDCMEISCRLCNQKSHIPLTCKEYAKDNKLDVRHRVEEAMTEALVRKCNKCSYPYIKEYGCNKIHCNRCNTLQCYICSQTIKNYDHFNDPARGGKIGNCPLFDNTEERHHDEVETAAKKAMEMLRVEHPELDDEDLKIELSERVRKEEEAKRSRAAAANGGIPLYQPQPARQPVAPPVRRRVPGPPPPAPPPAPQIGGPAAGAGHRGRLQRVRDRIQQVVQPQAPPPAQPPHVIMGHQLPLFHQVAQNMYPIPLPQYPPYPAPNQYPGAKPAQQPLIGGLPAAHGAPIVNERVPPPPPPPPPQPFRRAVLSRRNTVAAARPMGAEIQGMGVPIQEQNLMMAMGRAQAQAPAPPAGPPSGISAPRRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5S3</accession>
    <name>D5G5S3_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">tRNA-dihydrouridine(20a/20b) synthase [NAD(P)+]</fullName>
            <ecNumber evidence="17">1.3.1.90</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">tRNA-dihydrouridine synthase 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">GSTUM_00001458001</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs. Specifically modifies U47 in cytoplasmic tRNAs. Catalyzes the synthesis of dihydrouridine in some mRNAs, thereby affecting their translation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>5,6-dihydrouridine(20a) in tRNA + NAD(+) = uridine(20a) in tRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53348"/>
            <dbReference type="Rhea" id="RHEA-COMP:13535"/>
            <dbReference type="Rhea" id="RHEA-COMP:13536"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.90"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="13">
            <dbReference type="Rhea" id="RHEA:53350"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>5,6-dihydrouridine(20a) in tRNA + NADP(+) = uridine(20a) in tRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53344"/>
            <dbReference type="Rhea" id="RHEA-COMP:13535"/>
            <dbReference type="Rhea" id="RHEA-COMP:13536"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.90"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="15">
            <dbReference type="Rhea" id="RHEA:53346"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>5,6-dihydrouridine(20b) in tRNA + NAD(+) = uridine(20b) in tRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53352"/>
            <dbReference type="Rhea" id="RHEA-COMP:13537"/>
            <dbReference type="Rhea" id="RHEA-COMP:13538"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.90"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="14">
            <dbReference type="Rhea" id="RHEA:53354"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>5,6-dihydrouridine(20b) in tRNA + NADP(+) = uridine(20b) in tRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53356"/>
            <dbReference type="Rhea" id="RHEA-COMP:13537"/>
            <dbReference type="Rhea" id="RHEA-COMP:13538"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.90"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="12">
            <dbReference type="Rhea" id="RHEA:53358"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a 5,6-dihydrouridine in mRNA + NAD(+) = a uridine in mRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:69851"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:17789"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="10">
            <dbReference type="Rhea" id="RHEA:69853"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 5,6-dihydrouridine in mRNA + NADP(+) = a uridine in mRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:69855"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:17789"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="11">
            <dbReference type="Rhea" id="RHEA:69857"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the Dus family. Dus4 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.90" evidence="17"/>
    <dbReference type="EMBL" id="FN430001">
        <property type="protein sequence ID" value="CAZ79866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835709.1">
        <property type="nucleotide sequence ID" value="XM_002835663.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5S3"/>
    <dbReference type="FunCoup" id="D5G5S3">
        <property type="interactions" value="138"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G5S3"/>
    <dbReference type="GeneID" id="9187544"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001458001"/>
    <dbReference type="eggNOG" id="KOG2335">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_596097_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5S3"/>
    <dbReference type="OMA" id="VEPHCDY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102266">
        <property type="term" value="F:tRNA-dihydrouridine20a synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102267">
        <property type="term" value="F:tRNA-dihydrouridine20b synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02801">
        <property type="entry name" value="DUS_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000159">
        <property type="entry name" value="tRNA-dihydrouridine synthase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035587">
        <property type="entry name" value="DUS-like_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018517">
        <property type="entry name" value="tRNA_hU_synthase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11082">
        <property type="entry name" value="TRNA-DIHYDROURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11082:SF31">
        <property type="entry name" value="TRNA-DIHYDROURIDINE(20A_20B) SYNTHASE [NAD(P)+]-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01207">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01136">
        <property type="entry name" value="UPF0034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="DUS-like FMN-binding" evidence="20">
        <location>
            <begin position="33"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00050434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00051779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00051932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00052996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00060741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00066483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00071722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00078338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="CAZ79866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="459" mass="51477" checksum="879B91297C4F3783" modified="2010-06-15" version="1">MDEESEIPAREVVHYPLKLFDLAKSAGRVLYVQAPMVRYSKLPFRALVRDYGVDLCYTPMVSLKRLYWIASAKKLLLDFTTNASDRPLIAQFGAHSSIDFSRAAELVKPYTDGVDLNCGCPQSWAIQEGIGCRLMQQPQLVKEMVRAAKDRCGEDFCVSIKIRVHHDLRETVDFVKMVESSGVDYITIHGRRRSQRSSEPVNSEAIALVKSLAGVPIVANGDVFSLGDAERIVGITGVDGVMAARGLMENPALFAGFKKTPWGAVERFLHYNANYGPLPYQLALHNVGEMLRDMMTKKERAEMTRNSGSIVEILNWLKQRGIGRIYARSRVWKGSKNGENIIATRILKTSLSSRACDGFWVNPSPRSYVRNTTTGTRVLNYKYEKKRKSLLALFFGHHHRSDLSKRELTTSITNTLYQTWNSLISTPNGATIARNLRKKGANDVLPTPLKEGMPLALCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6C3</accession>
    <name>D5G6C3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00004420001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the GOT1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430009">
        <property type="protein sequence ID" value="CAZ80066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835909.1">
        <property type="nucleotide sequence ID" value="XM_002835863.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6C3"/>
    <dbReference type="FunCoup" id="D5G6C3">
        <property type="interactions" value="782"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G6C3"/>
    <dbReference type="GeneID" id="9187437"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004420001"/>
    <dbReference type="eggNOG" id="KOG1743">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1361301_0_0_1"/>
    <dbReference type="InParanoid" id="D5G6C3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000137">
        <property type="term" value="C:Golgi cis cisterna"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045176">
        <property type="entry name" value="Got1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007305">
        <property type="entry name" value="Vesicle_transpt_Got1/SFT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21493">
        <property type="entry name" value="CGI-141-RELATED/LIPASE CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21493:SF9">
        <property type="entry name" value="GOLGI TRANSPORT PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04178">
        <property type="entry name" value="Got1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="76"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="99"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="137"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="167"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ80066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="201" mass="21866" checksum="32EE55F4D42B06E0" modified="2010-06-15" version="1">MLDFELVGKQKVINFSYDPPVTSLKAPSSIDCPPSLSQIICPPTLAANSSSSILPTLRTAHSLLVPIMWLSDVQKFGVGFCAGGIFFFLFGIMMFFDRAMLAMGNILFVVGISLFMGPKKTFSFFARKEKFRGTACFLLGVGLILAKYPLIGFCIELYGILNLFGDFFGVIIGFLGSVPVIGPYLEGPLRRVTGATQQLPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6T5</accession>
    <name>D5G6T5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00002260001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430014">
        <property type="protein sequence ID" value="CAZ80228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836071.1">
        <property type="nucleotide sequence ID" value="XM_002836025.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6T5"/>
    <dbReference type="STRING" id="656061.D5G6T5"/>
    <dbReference type="GeneID" id="9182452"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002260001"/>
    <dbReference type="eggNOG" id="KOG1674">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023749_4_0_1"/>
    <dbReference type="InParanoid" id="D5G6T5"/>
    <dbReference type="OMA" id="HSHYEPT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20558">
        <property type="entry name" value="CYCLIN_ScPCL7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013922">
        <property type="entry name" value="Cyclin_PHO80-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615:SF117">
        <property type="entry name" value="PHO85 CYCLIN PHO80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08613">
        <property type="entry name" value="Cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="333"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="88"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="111"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="346"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="370" mass="39167" checksum="594067DC32A85AC5" modified="2010-06-15" version="1">MNPAPTALPGPSSSSTTTSSATITRSSSSKSLSPADFKSPSYPQYVESPSLGAWSPKGGQPIPKTTGNSPSTNSKRRSSRPQTPRPPSSSSSPSASRISSHRSAMNPPATPVAAHSTPSSSSALSPASKAPTTPSTSTERHSKSSSPPKRARHSDPLVNYLPSLYQHCPTQDLVYLISDMLLELVGLNDKIPLSGAGLTRFHSRAPPTISIPDYLSRLALHASLQPSILLSMVYYIDILSTHYPPFVVSSLTVHRFLITAATVATKGLCDSFLTNGFYAKVGGVSLMELNLLELEFLVRVGWRIVPKGEVLDEYYRSLVGRCQSRYAISVTTVGGHEESDTPPGPSSSSTASEPRQDASSSVGEGGEAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6V6</accession>
    <name>D5G6V6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00002294001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430017">
        <property type="protein sequence ID" value="CAZ80249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836092.1">
        <property type="nucleotide sequence ID" value="XM_002836046.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6V6"/>
    <dbReference type="GeneID" id="9182473"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002294001"/>
    <dbReference type="HOGENOM" id="CLU_2887454_0_0_1"/>
    <dbReference type="InParanoid" id="D5G6V6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="63" mass="6768" checksum="2EFD0A0D9D3DA35D" modified="2010-06-15" version="1">MASLVDKTEEQSILFDPQKLNTPQREDGTSPLQRTSSMAGTKLVNNWRQAVGGIAVAVLVAVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5G709</accession>
    <name>D5G709_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00004537001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CoA-transferase III family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430020">
        <property type="protein sequence ID" value="CAZ80302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836111.1">
        <property type="nucleotide sequence ID" value="XM_002836065.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G709"/>
    <dbReference type="STRING" id="656061.D5G709"/>
    <dbReference type="GeneID" id="9182493"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004537001"/>
    <dbReference type="eggNOG" id="KOG3957">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033975_0_1_1"/>
    <dbReference type="InParanoid" id="D5G709"/>
    <dbReference type="OMA" id="IIAGPYC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047369">
        <property type="term" value="F:succinate-hydroxymethylglutarate CoA-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10540">
        <property type="entry name" value="Crotonobetainyl-coa:carnitine coa-transferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.10">
        <property type="entry name" value="formyl-coa transferase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050483">
        <property type="entry name" value="CoA-transferase_III_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003673">
        <property type="entry name" value="CoA-Trfase_fam_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044855">
        <property type="entry name" value="CoA-Trfase_III_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023606">
        <property type="entry name" value="CoA-Trfase_III_dom_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48207">
        <property type="entry name" value="SUCCINATE--HYDROXYMETHYLGLUTARATE COA-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48207:SF3">
        <property type="entry name" value="SUCCINATE--HYDROXYMETHYLGLUTARATE COA-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02515">
        <property type="entry name" value="CoA_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89796">
        <property type="entry name" value="CoA-transferase family III (CaiB/BaiF)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ80302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="439" mass="47709" checksum="A2ADB4387D0A1434" modified="2010-06-15" version="1">MHLHRLSLFPGPRSLRLRLRGYSTSLSEAVDQSKSKLPLKGLRVLDLTRVLAGPYCTQILGDLGAEVIKVEHPTRGDDTRAWGPPYAPSKDGGKGESAYFLAVNRNKKSLGLSFKHPSGQYILQHLAKASDILVENYLPGTLKKYALDYPTLHEMAPHLIYASLTGYGQTGPYKDRTGYDVMVEAEMGLMHITGEPERPPVKVGVAVTDLTTGLYCSNSILAAVIARAGNGGVGQWIDVALSDCQVATLANIASSALISGKGDEGRFGTAHPSIVPYESFPTADGNILLGGGNDRLYAILCQKIGKPEWITDPRFATNEERVKNRKILVPMMVEITRSKTTQEWLEIFDSSGIPYAAVNDIQATLSHSHVIARDMVKTVHHPTCGDIKLVNTPVKYSDSTPGIRTPPPTLGQHTDEVLMDLLGLSSYAVEELRRDGVVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7G3</accession>
    <name>D5G7G3_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Glutamate synthase [NADH]</fullName>
            <ecNumber evidence="20">1.4.1.14</ecNumber>
            <ecNumber evidence="22">1.4.7.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="28" type="ORF">GSTUM_00002451001</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="28 29">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="24">Forms L-glutamate from L-glutamine and 2-oxoglutarate. Represents an alternative pathway to L-glutamate dehydrogenase for the biosynthesis of L-glutamate. Participates with glutamine synthetase in ammonia assimilation processes. The enzyme is specific for NADH, L-glutamine and 2-oxoglutarate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>2 L-glutamate + NAD(+) = L-glutamine + 2-oxoglutarate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13753"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="1.4.1.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Amino-acid biosynthesis; L-glutamate biosynthesis via GLT pathway; L-glutamate from 2-oxoglutarate and L-glutamine (NAD(+) route): step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="21">Amino-acid biosynthesis; L-glutamate biosynthesis via GLT pathway; L-glutamate from 2-oxoglutarate and L-glutamine (ferredoxin route): step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Energy metabolism; nitrogen metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Nitrogen metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the glutamate synthase family.</text>
    </comment>
    <dbReference type="EC" id="1.4.1.14" evidence="20"/>
    <dbReference type="EC" id="1.4.7.1" evidence="22"/>
    <dbReference type="EMBL" id="FN430027">
        <property type="protein sequence ID" value="CAZ80456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836265.1">
        <property type="nucleotide sequence ID" value="XM_002836219.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5G7G3">
        <property type="interactions" value="595"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G7G3"/>
    <dbReference type="GeneID" id="9188481"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002451001"/>
    <dbReference type="eggNOG" id="KOG0399">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000422_8_2_1"/>
    <dbReference type="InParanoid" id="D5G7G3"/>
    <dbReference type="OMA" id="RVMGPEQ"/>
    <dbReference type="UniPathway" id="UPA00045"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051538">
        <property type="term" value="F:3 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016041">
        <property type="term" value="F:glutamate synthase (ferredoxin) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016040">
        <property type="term" value="F:glutamate synthase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019676">
        <property type="term" value="P:ammonia assimilation cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006537">
        <property type="term" value="P:glutamate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00982">
        <property type="entry name" value="gltB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00713">
        <property type="entry name" value="GltS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02808">
        <property type="entry name" value="GltS_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000031">
        <property type="entry name" value="Glutamate synthase 1 [NADH]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000017">
        <property type="entry name" value="Glutamate synthase [NADH], amyloplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.60:FF:000001">
        <property type="entry name" value="Glutamate synthase, large subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000001">
        <property type="entry name" value="Glutamate synthase, large subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.60">
        <property type="entry name" value="Glutamate synthase, alpha subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050711">
        <property type="entry name" value="ET-N_metabolism_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002489">
        <property type="entry name" value="Glu_synth_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036485">
        <property type="entry name" value="Glu_synth_asu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006982">
        <property type="entry name" value="Glu_synth_centr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002932">
        <property type="entry name" value="Glu_synthdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008730">
        <property type="entry name" value="PRK11750.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11938">
        <property type="entry name" value="FAD NADPH DEHYDROGENASE/OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11938:SF133">
        <property type="entry name" value="GLUTAMATE SYNTHASE (NADH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00310">
        <property type="entry name" value="GATase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04898">
        <property type="entry name" value="Glu_syn_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01645">
        <property type="entry name" value="Glu_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01493">
        <property type="entry name" value="GXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69336">
        <property type="entry name" value="Alpha subunit of glutamate synthase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="19" id="KW-0003">3Fe-4S</keyword>
    <keyword evidence="9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="13" id="KW-0274">FAD</keyword>
    <keyword evidence="10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="11" id="KW-0288">FMN</keyword>
    <keyword evidence="18" id="KW-0314">Glutamate biosynthesis</keyword>
    <keyword evidence="14" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="16" id="KW-0408">Iron</keyword>
    <keyword evidence="17" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="27">
        <location>
            <begin position="1"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="1468"/>
            <end position="1496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00009716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00024383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00037928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00039085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00057049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00068518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="EMBL" id="CAZ80456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1496" mass="164756" checksum="E740B08D0DDB9124" modified="2010-06-15" version="1">MTHRGAVGSDARDGDGAGVMTSIPHKFFVKEFTGSSGFVLPPQGQYAVGNLFFKPSEEVLIESKNTFIELADSLGLRVLGWREVPRDSALLGPAALSREPTILQPFVVLRSRYGEGEKPENTDAESFDEKYFERQLFILRKVSTKKIGLHNWFYICSLSNKNIVYKGQLAPVQVYTYYHDLVNVDYAVHFALVHSRFSTNTFPSWDRAQPLRWVAHNGEINTLRGNKNWMRAREGQMKSAKFGEELEQLYPIIEEGGSDSAAFDNVLELLTINGVLSLPETIMMMVPEAWQDNALMDPKKVAFYEWAACLMEPWDGPALFTFADGRYCGATLDRNGLRPCRFYVTDDDRIICASEVGTILIDPEHIIQKGRLKPGRMLLVDTVEGRIVDDKELKMGVSSRFDFTSWVEEQLLTIKKVYNQLESSGVRLEPSLDDNSLQNDPRLLAFGYSFEQVSLILGPMAADSKEALGSMGNDAPLACLAKQPRLLYEYFRQLFAQVTNPPIDPIREAIVMSLECYVGPQGNVLEMGPSQCHRLLLPSPVLSPNEFNALTSIQQVNNSWTVHTIDITFPKEQGVPGYINALDEVCMQASKAIEKGDKVLVLSDRNTSADRVPLSALLACGGVHHHLVRNRMRSKIALVIETAEAREVHHLCVLLGYGADAICPYLAMECILKMNREKLIRGGLSDEKVVDNFKHSCDGGILKVMSKMGISTLQSYKGAQIFEALGVDDSVVDRCFTGTATRIKGITLDFIAQDAFALHETGYPSRKIVSIPGLPETGEYHWRDGGESHVNDPVSIANIQDAVRTKNDKSYEAYSLSEYEQIKNCTLRGLLDFDFSSSKPIPIDQVEPWTEIVRRFCTGAMSYGSISMESHSTLAVAMNRLGGKSNTGEGGEDPERSQILENGDTMRSAIKQVASGRFGVTSHYLADSDELQIKMAQGAKPGEGGELPGHKVSQSIARTRHSTPGVGLISPPPHHDIYSIEDLKQLIYDLKCANPRSRVSVKLVSETGVGIVASGVAKAKADHILISGHDGGTGASRWTGIKYAGLPWELGLAETHQTLVLNDLRGRVIVQTDGQLRTGRDVAIACLLGAEEWGFATTPLIAMGCIMMRKCHLNTCPVGIATQDPELRKKFTGTPENVINFFYYVANELRAIMARLGFRTINEMIGRSEKLLVRRDLMTEKTNNIDLSLILTPAHTLRPGVATYNVRKQDHRLHVRLDNKLIDESEYTLEKGIPTRIESDIVNTDRALGATLSYRISRKMGEAGLEPDSVHVNLRGSAGQSFGAFLAPGVTLELEGDANDYVGKGLSGGRVVIYPPRNATYKAEENIIVGNVCLYGATAGQCFFRGVAAERFAVRNSGATAVVEGVGDHGCEYMTGGRVIILGETGRNFAAGMSGGIAYVLDIKQNFASKVNMEMVELSGVEDAHEISWVRGLVEDHYQFTGSELAKRILLDYRRALPRFSSGRGSTKIEGLPIPIRSTSRQPGGGRQAQACGCYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7U1</accession>
    <name>D5G7U1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00004723001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430031">
        <property type="protein sequence ID" value="CAZ80584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836393.1">
        <property type="nucleotide sequence ID" value="XM_002836347.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7U1"/>
    <dbReference type="GeneID" id="9181403"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004723001"/>
    <dbReference type="HOGENOM" id="CLU_2777729_0_0_1"/>
    <dbReference type="InParanoid" id="D5G7U1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ80584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="69" mass="7721" checksum="3483ECA4950A250A" modified="2010-06-15" version="1">MSGSGVSRGSGPALDAELDELEGSKEEYHIQCDLTIFGGGKWVCVRYDRLFSMRVRCFLHHRVVSLSCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7U5</accession>
    <name>D5G7U5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 15">Dolichyl-phosphate-mannose--protein mannosyltransferase</fullName>
            <ecNumber evidence="4 15">2.4.1.109</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">GSTUM_00002656001</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Transfers mannose from Dol-P-mannose to Ser or Thr residues on proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-seryl-[protein] = 3-O-(alpha-D-mannosyl)-L-seryl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17377"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:13546"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137321"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 15">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-threonyl-[protein] = 3-O-(alpha-D-mannosyl)-L-threonyl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53396"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:13547"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137323"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 15">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Endoplasmic reticulum membrane</location>
            <topology evidence="1 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the glycosyltransferase 39 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.109" evidence="4 15"/>
    <dbReference type="EMBL" id="FN430032">
        <property type="protein sequence ID" value="CAZ80588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836397.1">
        <property type="nucleotide sequence ID" value="XM_002836351.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7U5"/>
    <dbReference type="FunCoup" id="D5G7U5">
        <property type="interactions" value="50"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G7U5"/>
    <dbReference type="GeneID" id="9181407"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002656001"/>
    <dbReference type="eggNOG" id="KOG3359">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008438_2_2_1"/>
    <dbReference type="InParanoid" id="D5G7U5"/>
    <dbReference type="OMA" id="KNVTPRL"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031502">
        <property type="term" value="C:dolichyl-phosphate-mannose-protein mannosyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004169">
        <property type="term" value="F:dolichyl-phosphate-mannose-protein mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd23283">
        <property type="entry name" value="beta-trefoil_MIR_PMT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000034">
        <property type="entry name" value="Dolichyl-phosphate-mannose-protein mannosyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003342">
        <property type="entry name" value="ArnT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036300">
        <property type="entry name" value="MIR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016093">
        <property type="entry name" value="MIR_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027005">
        <property type="entry name" value="PMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032421">
        <property type="entry name" value="PMT_4TMC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050">
        <property type="entry name" value="DOLICHYL-PHOSPHATE-MANNOSE--PROTEIN MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050:SF50">
        <property type="entry name" value="DOLICHYL-PHOSPHATE-MANNOSE--PROTEIN MANNOSYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02815">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02366">
        <property type="entry name" value="PMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16192">
        <property type="entry name" value="PMT_4TMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00472">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82109">
        <property type="entry name" value="MIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50919">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 15" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="126"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="164"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="222"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="277"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="307"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="629"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="671"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="700"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="729"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="17">
        <location>
            <begin position="360"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="17">
        <location>
            <begin position="429"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="17">
        <location>
            <begin position="499"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="889"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="27"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU367007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAZ80588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="956" mass="107607" checksum="151D0501D1B8D18E" modified="2010-06-15" version="1">MSTKPPARLTVDEKGYDRARSRSPSPSRRKKANKSKKREDGPLSMRDSSFRKQDTSSGERAPRQMSKDNDIFLLPSSDYQILGVLTVIGVCVRLFKIYQPSSVVFDEVHFGGFATKYIKGRFFMDVHPPLAKLLITLAGWLAGFDGGFDFKEIGKDYLGPSVPYVAMRMLPAVMGILVVPIIFLTLKAAGNSTATASLGAGLVIFENGLITQSRLILLDSPLVTFTALTALAWTSFLNIHEQGASKAFGPNWWLWLAMTGLGLGATVSVKWVGLFTIAWVGSLTVLQLWFLLGDTKNVTPRLWFKHFLARVFCLIVIPLGFYMLMFAIHFLCLVNPGDGDGFMSSEFQATLNSKGMHSVPADVAFGSRISIRHHNTQGGYLHSHNHMYPAGSKQQQITLYPHKDENNQWLLENETNPVTGIEGYDIITPPNLVFDDATIKLYHISTDRRLHSHDVRPPVTEEEWQNEVSAYGYKGFEGDANDLFRIEIVKHLSDGPEAKKRLRTIQTKFRLVHVMTGCALFSHKVKLPDWGFEQQEVTCAKGATLPNSVWYIENNEHPQLQEGAELVNYKNPGFFGKFWELQKVMWRTNAGLVESHAWDSRPPSWPVLRRGINFWGKDHRQIYLIGNPIIWWSSTAAIAVYLAFKAFAVLRWQRGYADYSLTNFRRFDFEVGITVLGWGLHYFPFYLMERQLFLHHYFPALYFAIIAFCQVFDFITTRTGALGLNRRPAIGWTIATVFLGLSIAAFTLYAPLAYGSPWTKAQCNRVKLFENWDWDCNLFYNSMSEYDNIVATPSEALTSTPVPVVADPHNFQESEGKAQQPNTTPLAEELKEAHQIPAAGMPEGQRVVAQEERIEYRDQDGNILNEEQVKALEGKVEFHTRYETRTRLLDEAGNEVPAESSGVAGPLVDGANPETPKMEPLESGLPPRVDAETESEVDAERRRAEPSPASDGLHET</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7V0</accession>
    <name>D5G7V0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00002665001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430032">
        <property type="protein sequence ID" value="CAZ80593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836402.1">
        <property type="nucleotide sequence ID" value="XM_002836356.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7V0"/>
    <dbReference type="GeneID" id="9181412"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002665001"/>
    <dbReference type="HOGENOM" id="CLU_2401277_0_0_1"/>
    <dbReference type="InParanoid" id="D5G7V0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ80593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="93" mass="10087" checksum="E3817E9333BBDAAF" modified="2010-06-15" version="1">MVVLHHVSLARFLPRSGLNAPNPSLFTPCLPLHEGYNFSGISSAGQLGTTLHRSLAIRVALQSGWRELRAGRNPWKIGDGSAQSRGHVGFIMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7W1</accession>
    <name>D5G7W1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 18">Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial</fullName>
            <ecNumber evidence="5 18">1.3.5.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">GSTUM_00002676001</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a quinone + succinate = fumarate + a quinol</text>
            <dbReference type="Rhea" id="RHEA:40523"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.3.5.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
        <text evidence="18">Binds 1 [2Fe-2S] cluster.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </cofactor>
        <text evidence="18">Binds 1 [3Fe-4S] cluster.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="18">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 18">Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (eukaryal route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of complex II composed of four subunits: a flavoprotein (FP), an iron-sulfur protein (IP), and a cytochrome b composed of a large and a small subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 18">Mitochondrion inner membrane</location>
            <topology evidence="1 18">Peripheral membrane protein</topology>
            <orientation evidence="1 18">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.1" evidence="5 18"/>
    <dbReference type="EMBL" id="FN430032">
        <property type="protein sequence ID" value="CAZ80604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836413.1">
        <property type="nucleotide sequence ID" value="XM_002836367.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7W1"/>
    <dbReference type="FunCoup" id="D5G7W1">
        <property type="interactions" value="662"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G7W1"/>
    <dbReference type="GeneID" id="9181423"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002676001"/>
    <dbReference type="eggNOG" id="KOG3049">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044838_0_0_1"/>
    <dbReference type="InParanoid" id="D5G7W1"/>
    <dbReference type="OMA" id="DGQYFGP"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER01006"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051538">
        <property type="term" value="F:3 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008177">
        <property type="term" value="F:succinate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1060.10:FF:000001">
        <property type="entry name" value="Succinate dehydrogenase iron-sulfur subunit SdhB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="entry name" value="Alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006058">
        <property type="entry name" value="2Fe2S_fd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050573">
        <property type="entry name" value="SDH/FRD_Iron-Sulfur"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004489">
        <property type="entry name" value="Succ_DH/fum_Rdtase_Fe-S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025192">
        <property type="entry name" value="Succ_DH/fum_Rdtase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00384">
        <property type="entry name" value="dhsB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11921:SF29">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE [UBIQUINONE] IRON-SULFUR SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11921">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE IRON-SULFUR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13085">
        <property type="entry name" value="Fer2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13534">
        <property type="entry name" value="Fer4_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46548">
        <property type="entry name" value="alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00197">
        <property type="entry name" value="2FE2S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 18" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="16 18" id="KW-0003">3Fe-4S</keyword>
    <keyword evidence="8 18" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="12" id="KW-0249">Electron transport</keyword>
    <keyword evidence="14 18" id="KW-0408">Iron</keyword>
    <keyword evidence="15 18" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="18" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="18" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="19">
        <location>
            <begin position="50"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="20">
        <location>
            <begin position="162"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00016766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU361237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="CAZ80604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="260" mass="29342" checksum="90CE92A94C515BEE" modified="2010-06-15" version="1">MNVTFLSRSLRRATLTSLKLTTGLRTLASVTASQTVPAGSGSKKENIKQFQIYRWNPDTPEKKPYMQTYELDMDQTGPMVLDALIRLKNEVDPTLTFRRSCREGICGSCAMNIQGSNTLACLCRIDVSNSAPFYKQYKSIKPYLQRKTPSPDGREYRQSVEERKKLDGLYECILCACCSTSCPSYWWNSSDYLGPAVLLQSYRWLADSRDEAKGARKSALENEMSLYRCHTIMNCTRTCPKGLNPGRAIANIKKEMAFGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5G891</accession>
    <name>D5G891_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00002934001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430041">
        <property type="protein sequence ID" value="CAZ80734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836543.1">
        <property type="nucleotide sequence ID" value="XM_002836497.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G891"/>
    <dbReference type="GeneID" id="9188336"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002934001"/>
    <dbReference type="HOGENOM" id="CLU_1476185_0_0_1"/>
    <dbReference type="InParanoid" id="D5G891"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ80734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="183" mass="20328" checksum="20FE92DBA42024CE" modified="2010-06-15" version="1">METQLLRGSSVNQVVKFYWSFYCKLFVCCFSLDHAGHTLSMSYKYTALRAESEEKHACPGNCIAGCRPILIPARPIQIQLPTCTCNSRATTPIFAQPGALRNTGVTRKKVSPRKRRCLHIKGFSFFSNLHPSLSFAGHGHVELHHNTPGFRCLLGCLSQDFRSEPSFKYLGTSLGGVVPIHGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8G4</accession>
    <name>D5G8G4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00002853001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="FN430043">
        <property type="protein sequence ID" value="CAZ80807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836616.1">
        <property type="nucleotide sequence ID" value="XM_002836570.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8G4"/>
    <dbReference type="STRING" id="656061.D5G8G4"/>
    <dbReference type="GeneID" id="9186026"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002853001"/>
    <dbReference type="eggNOG" id="KOG0332">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2198908_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8G4"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF1">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ80807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="108" mass="11905" checksum="AD46F3A31845C751" modified="2010-06-15" version="1">MSVNDVQALCLAPTRELARQILSVVQTMGQFTNIKTQLAIPNMVPRGQRIDAHIVVGTPGPVLDLIRRKELAVEYLKVLVLDEADNMLDLQGLGEQCLSVKRNIPSNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8G9</accession>
    <name>D5G8G9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00002859001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="FN430043">
        <property type="protein sequence ID" value="CAZ80812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836621.1">
        <property type="nucleotide sequence ID" value="XM_002836575.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8G9"/>
    <dbReference type="STRING" id="656061.D5G8G9"/>
    <dbReference type="GeneID" id="9186031"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002859001"/>
    <dbReference type="eggNOG" id="KOG0332">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_1_0_1"/>
    <dbReference type="InParanoid" id="D5G8G9"/>
    <dbReference type="OMA" id="FECAIGG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="16"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="183"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ80812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="352" mass="39170" checksum="EFEA6C541E8A5577" modified="2010-06-15" version="1">MNFKRPSKIQERALPLLLSDPRKNLIAQLQSGTGKTAAFALTMLTRVDMSVRDVQALCLAPTRELAGQIPGAVQKMGQFINIKTQLAIPNMVRRGQRMDAHIVVGTPGTVLDLIRCKELAVEHLKILVLDEAGNMLDLQGLGEQCLRVKQNTPPNTQIALISATFPHQQPCMDSDSKEDKYRILIDLYHILTIRSSVILLQNRETAYQIQRRMEADGHKVASLHSAEDGPDRDKVFNTSRSGKAKVLITTDVLVRGFDVSTVSMVVNYYLPRGKNRRLDAKTYLHRIGRTGRFGRVGVAITFAHGKQTLNEVGEISNRFGVCITRVPTNDIEEVEKIVNSVLKNKKAGGTKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>D5G902</accession>
    <name>D5G902_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00004920001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430053">
        <property type="protein sequence ID" value="CAZ80995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836804.1">
        <property type="nucleotide sequence ID" value="XM_002836758.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G902"/>
    <dbReference type="GeneID" id="9181278"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004920001"/>
    <dbReference type="HOGENOM" id="CLU_1120803_0_0_1"/>
    <dbReference type="InParanoid" id="D5G902"/>
    <dbReference type="OMA" id="SARYVWR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="248" mass="27414" checksum="41D2BCC53777C29A" modified="2010-06-15" version="1">MSSRNNSVSTTASYNTNASSSTAATSISARSSEDRNTILSDFNPTVVLYVPPRGSLPSSRNTIPLHDLTPYIKVLDRENLTKKQLRTLHAAKPWEKGGGDDEQGLVSRRMGLGSGRSVLGESVEWRVPWDVMKPAKIKSGGEELDVWCGKEDRRAQLFDVGSARYVWRFNEGSDQVLLEKHMTDTRSRHYIGAFYIKITPFPALGPLTSIGGTLILDERGLDKLTAIASVLVLLKRERQRRRVGGGTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9H6</accession>
    <name>D5G9H6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00003404001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430061">
        <property type="protein sequence ID" value="CAZ81169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836978.1">
        <property type="nucleotide sequence ID" value="XM_002836932.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9H6"/>
    <dbReference type="FunCoup" id="D5G9H6">
        <property type="interactions" value="21"/>
    </dbReference>
    <dbReference type="GeneID" id="9182920"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003404001"/>
    <dbReference type="eggNOG" id="ENOG502S4CU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097653_0_0_1"/>
    <dbReference type="InParanoid" id="D5G9H6"/>
    <dbReference type="OMA" id="HICAMYG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0085020">
        <property type="term" value="P:protein K6-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171:SF8">
        <property type="entry name" value="BRCA1-ASSOCIATED RING DOMAIN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="89"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ81169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="177" mass="19018" checksum="F2F0D94A4C451F19" modified="2010-06-15" version="1">MAAISRGAEEEQGASLKELLLESARRNNTDLLEEVISEGASGLADLLNNTRDGVGNTAVHLAAKNGSLEVLDLLLDQEGLEVDPLDRMEKETPLHKAVLYAKSREEGGLEVVEMLIDAGADPRIRNKAKQRPVDIVDPRDTELRVVLQKAEYLMTAGGDIVPESDDEGDGGSASDSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9H7</accession>
    <name>D5G9H7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00003405001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430061">
        <property type="protein sequence ID" value="CAZ81170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9H7"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003405001"/>
    <dbReference type="HOGENOM" id="CLU_2814278_0_0_1"/>
    <dbReference type="InParanoid" id="D5G9H7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="67" mass="7611" checksum="23EEA6CFAC68B430" modified="2010-06-15" version="1">MTAAPPVQKDHQPQLPKGTKEMHQKALAEIELQQAKLREMIASKRAEKETLQAVYERLKKRGDDGKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9Q2</accession>
    <name>D5G9Q2_TUBMM</name>
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        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00005021001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="CAZ81245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002837008.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9Q2"/>
    <dbReference type="STRING" id="656061.D5G9Q2"/>
    <dbReference type="GeneID" id="9185382"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005021001"/>
    <dbReference type="eggNOG" id="ENOG502QRXF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010063_1_0_1"/>
    <dbReference type="InParanoid" id="D5G9Q2"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010816">
        <property type="entry name" value="Het-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052577">
        <property type="entry name" value="VWA7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14905">
        <property type="entry name" value="NG37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14905:SF11">
        <property type="entry name" value="TINC (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07217">
        <property type="entry name" value="Het-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
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        <location>
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            <end position="969"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="739"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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        </location>
    </feature>
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            <begin position="566"/>
            <end position="576"/>
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    </feature>
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            <end position="794"/>
        </location>
    </feature>
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        <location>
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            <end position="855"/>
        </location>
    </feature>
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            <end position="902"/>
        </location>
    </feature>
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        <location>
            <begin position="915"/>
            <end position="924"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAZ81245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="969" mass="106672" checksum="EBCA0582BFF4CFE6" modified="2010-06-15" version="1">MAGSTFSSPMLLPLCLVLIYAALPAHAFGAGNIASVSTVEGQNWRHGDIEDALLSISMAAVAGGKRFSFMDVKRVYFGNWLRDYSQAVDVGALKYVEGPTVRLLLSILSFMTFGYATGEFEVTDERLGCYRPEEHIDNPKDYADNEDARRYDPRLRGPVDEATELSVDPQTGMKNYIANERIRNLRSGDPMCTSAGLVRHLFGRSIQLARNYGQTGNKAELYEALRLMGTGLHCLEDFSAHSNYIELALIELGETDVFPHVGSRTRCEVNGRMIYPLVTGTFGGVDFLHSVLGEASDKLIQSEVEQLESTITAASAQSSKDGGEHGIIRDLLKQLKIGGSDDLDAQATNLEQQSEAKKNEPWGISNGGAGIKREIMPFLEWHDKIMKAINEALESIPGLTALVEKLSEAVSVFVFSLLAPYILPIVAQAKGELANGSGEVIGGARAKQFVVFEDHYSSDPTHSMLSKDHFTNLLNEPAGKVASETVKFVVPLLMQAWDGNSDPNWVLDQIIPIFHHPAFVDGSDGCGRQEGRHRMFGIIRQWWEEMDESRKQFYRRSLSGQGVEQGKNHKEGHDSGHGCGKPIHRTKPKNSGGEFCGIGEDLGKAMGGAIEQALTGSQSGGSGLGGFLGGVAATALGETLLGGSASGGRNESYSERYDDGNARSADRSYSHSGRERGQRRGEGEYGMQVAGDSHDGGKGYAAAVYSAGGEYGYSEQRYGQNYSRVPIYGGVEYGSGDYDGGESFVVPDHDHHHHHHHHHHHQPEGHSHHHHHYHEQPTYERTDSYGSNHPTDTPSYSRTSSYASESSYSVPAAEYPYIHPHGQYGEYPTNASYRTETYTQSSSYGGGQSSYQTSTRQYPGSRETREVVYSGEGDEGYVVERSYKKYSDGEEERKYKSYRRGEESEDECEEEERRKKEKKWKKKHEGSGDEGSGDEYYGSGDEDGGRRYSGSGDEDGGRRRYSGSGDEYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5GA31</accession>
    <name>D5GA31_TUBMM</name>
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        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00003547001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMCO4 family.</text>
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        <property type="nucleotide sequence ID" value="XM_002837137.1"/>
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    <dbReference type="KEGG" id="tml:GSTUM_00003547001"/>
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    <dbReference type="OMA" id="KEVGWEV"/>
    <dbReference type="Proteomes" id="UP000006911">
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    <dbReference type="GO" id="GO:0016020">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035650">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="P:clathrin-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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        <property type="entry name" value="DUF726"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029024">
        <property type="entry name" value="TerB-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17920:SF3">
        <property type="entry name" value="TRANSMEMBRANE AND COILED-COIL DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17920">
        <property type="entry name" value="TRANSMEMBRANE AND COILED-COIL DOMAIN-CONTAINING PROTEIN 4 TMCO4"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF05277">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <location>
            <begin position="455"/>
            <end position="477"/>
        </location>
    </feature>
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        <location>
            <begin position="17"/>
            <end position="106"/>
        </location>
    </feature>
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    </feature>
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        </location>
    </feature>
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            <end position="50"/>
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
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        </location>
    </feature>
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        <location>
            <begin position="950"/>
            <end position="966"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ81374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1051" mass="115330" checksum="8D3EA73498544F73" modified="2010-06-15" version="1">MGTEVEYDEFGLPVVRLKVSTPPPPSSPSALLVEGKGKGEEKVGEPRTEEVEPTGSEQEKSTVSTDADSTISPATNTLASQNVATDVAPPMAPKESANKTGLANEAAAAVVVKDQVVRPSADIPQADREWNEKKMFSEDPADPSSTVGTGGPVSEWSHQQIVRREEEKDKDRGGAEEEDDGWQEMPAIASHDLYDDEGRLIAKEAQDSEDEGEKSASKGYTRVYDDEDAQSATSMDENTSYLFKETEDDEASRNPLSQMQATKELLTEGQRIAYVGVCKLAMERMVTDQGKLKGKGRSVKKELQLSAESLKMWSQKMMVRLYMHMDISSAEQVMIEQLAEHGVIPADLTSALAKNSRPGVKNPTEISNSKTLDIDIRWTVLCDLFLVLIADSVYDARSRVLLEKVGEMLGVPWLDIYKFEKRVTDALEIQESAQQNWSEEEHMENRRKAARNKRFMMMGLATIGGGLVIGLSGGLLAPVIGAGLAAGFTTIGVGGTGAFLAGAGGTALIASSAVASGSVIGAKASYNRTKSVGIFEYRPLHNAKRVNLIITVSGWMNGSEDDVRLPYSTVDPVMGDIFSILWEPEMLRSMGQTINILATEVLTQSLQQVLGSTILVALMASLQLPVVLTKLSYLLDNPWSVSLDRANAAGLILADSLIHRNLGVRPVTLVGYSLGARVIYSCLRELQRHHAYGLVQNVYLFGSPVVVKQDEYAKALSVVSGRFVNGFARNDWILGYLFRATSGGIGRVAGLAPVDNIVGVENVDVTELVNGHMAYRQAIPKLLRHVGWIVVSDEFTEIQDPAGLPIDPDRHRERQRELIEELEGARKELEKQQKKPRFSFFGRGKDAAKKKDWETYDEVKPANDPGMEKDRVDPNDPNTGIKDDNVIFDVDAIRAELAKVGQDEIQPRELNSTLPPLKADTSLLNSPMQANLRHTKSFDHRSSSLYGMSSISENNASLPSLQNGHHQPTPLGDKNNRTYNSDSEDEFGNPRTGEIQMTFDAPSASPRNIALFHRPEIRTSVTMPTTTNERNVWADDDDDFGEEKEMTMTFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAH7</accession>
    <name>D5GAH7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00003592001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430077">
        <property type="protein sequence ID" value="CAZ81520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837329.1">
        <property type="nucleotide sequence ID" value="XM_002837283.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAH7"/>
    <dbReference type="GeneID" id="9187460"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003592001"/>
    <dbReference type="HOGENOM" id="CLU_750461_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAH7"/>
    <dbReference type="OMA" id="ANWAFND"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF47">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="115"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="145"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="195"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="228"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="285"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="8">
        <location>
            <begin position="107"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="330"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="333"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ81520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="369" mass="40741" checksum="4DDAA3F7073550E6" modified="2010-06-15" version="1">MHELSNWGHVVLIWTLVLVLYIVLGLRTWALRNTNYSMFSPRGISTLCIALTVALTTAVCAVVTWSLVGPMHGVPKRVLTSLYNPDLPESGDIFFGPDPRAPLETVFKVNFGSMILFYVVLWLIKVSFVVIYFEFSKDLSRQTRTLLYITIAAVAVTFVFVICLYSLWCLPVSANWAFNDLVQSGTCRASLAPSVIISVINVLVDILIMVAGFSIVRSLNLGRSNFRSASLIIAVGILTVVVAFIRMIVLVVRMKNRDRAYDGIYNDAVHDKDTMNVYVPIQESVVGYAEVEAILACIAACLPGIRVLFRKHISRRSELTFKDCGSAGTLTPPASPPTACPSPPTSHLPFIPATPPPVYQQKVRVEEMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAU8</accession>
    <name>D5GAU8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00005314001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430086">
        <property type="protein sequence ID" value="CAZ81641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837450.1">
        <property type="nucleotide sequence ID" value="XM_002837404.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAU8"/>
    <dbReference type="STRING" id="656061.D5GAU8"/>
    <dbReference type="GeneID" id="9187809"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005314001"/>
    <dbReference type="eggNOG" id="ENOG502SDK4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_408366_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAU8"/>
    <dbReference type="OMA" id="HELEFRT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21552">
        <property type="entry name" value="VEFS-box_ctSUZ12-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019135">
        <property type="entry name" value="Polycomb_protein_VEFS-Box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22597">
        <property type="entry name" value="POLYCOMB GROUP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22597:SF0">
        <property type="entry name" value="POLYCOMB PROTEIN SUZ12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09733">
        <property type="entry name" value="VEFS-Box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zinc finger PHD-type" evidence="8">
        <location>
            <begin position="622"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="30"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ81641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="673" mass="77176" checksum="35225ADD22F0A5C3" modified="2010-06-15" version="1">MVEVLSEPPIPTGGKMEPVLKIEAKSLRRKWAPKPRVHPPSSGTRNSRTSRDRRSFDKPINIRCRCELTIYEKLDPLRAAKLSNVRRTRSRSREESSTNPLVRYRQFCTIAYDSEEFATIEMQDPFYIRAGELFVFTNRGPIQSFGLAESYTAEFALQPISTDQPWPPLDSNSSGRWDDPTNVQLVGVFHSLPMAPASASHELEFRTGILGETGERADIELELDCKWDKQGTITRGGEAYQLVSPRLPTPVSERGRDFSPVDSVLTCYVFSSPVDEERFHPCIEQPTIKHQHAFTVPRYICPFCDGRELTNLTSLHFHLITSHDLFQFKVRPNKAKSGLRGNERHENYVEVFVDLARDAMVGRASDHVPDHRTFRWVKPSEILDVDRILDGDWSWLNERRGISLQNRRDTTAGGTLVPFSKKRINLNEVGDLPVNKRRKYRVPVPMNGLQGLVFIRSRSKRFVYPDEELSESDDDVDEGWLQMEHKEIIEDFTDVARNEMTFMQLWDRHLFLERPIGYSHIPQVLIRFAREQAENMRTSQLLVEFWKHCLNLVQYGIIDTECVAFCMRVIKGEGQWSSAGQTKWGIGLSGPLVLDTQEAAPTKVEDTDVIDCLQAPEIISGFCTCGKPFDKPEMVLCNGNKCDTRWFHRSCVDAERMQEGWRCKICQEVQSGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5GB90</accession>
    <name>D5GB90_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00003797001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430087">
        <property type="protein sequence ID" value="CAZ81783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837592.1">
        <property type="nucleotide sequence ID" value="XM_002837546.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GB90"/>
    <dbReference type="FunCoup" id="D5GB90">
        <property type="interactions" value="427"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GB90"/>
    <dbReference type="GeneID" id="9181493"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003797001"/>
    <dbReference type="eggNOG" id="KOG2536">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072692_0_0_1"/>
    <dbReference type="InParanoid" id="D5GB90"/>
    <dbReference type="OMA" id="RWLNNVK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.280.10">
        <property type="entry name" value="Mitochondrial glycoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003428">
        <property type="entry name" value="MAM33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036561">
        <property type="entry name" value="MAM33_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10826">
        <property type="entry name" value="COMPLEMENT COMPONENT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10826:SF1">
        <property type="entry name" value="COMPLEMENT COMPONENT 1 Q SUBCOMPONENT-BINDING PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02330">
        <property type="entry name" value="MAM33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54529">
        <property type="entry name" value="Mitochondrial glycoprotein MAM33-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="144"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="307" mass="34294" checksum="CA2E4C064344CA2E" modified="2010-06-15" version="1">MFYARAVSRLWRAPSARVFLSLSISTPRSQSSAFLRPASVAQHPRLASRFSTTNSRFEKLGQVDEELALKLLTEIDLEKSKDEAEYPLSVKEYLETGQFEIIDKPGQEEVQLVRRFGDEIIRVEFSISDLNALANEGLNNDILYDGQPGEGDHNALSSQSGGVQSKAAQGEGRALAANDTDSDEYDDDPEPGFPARVNVTIEKPNSGALQIEAIAQDGMIVIDNVFYHKSAKLATAQTAEADWERRGIYAGPPFGNLDEDLQVLLERYLDERGINTALALFVPDYIDYKEQREYIQWLESVHNFVSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBA9</accession>
    <name>D5GBA9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00000395001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430090">
        <property type="protein sequence ID" value="CAZ81802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837611.1">
        <property type="nucleotide sequence ID" value="XM_002837565.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBA9"/>
    <dbReference type="FunCoup" id="D5GBA9">
        <property type="interactions" value="82"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GBA9"/>
    <dbReference type="GeneID" id="9181513"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000395001"/>
    <dbReference type="eggNOG" id="KOG4288">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055314_1_0_1"/>
    <dbReference type="InParanoid" id="D5GBA9"/>
    <dbReference type="OMA" id="WERADIF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051207">
        <property type="entry name" value="ComplexI_NDUFA9_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12126:SF16">
        <property type="entry name" value="MIOREX COMPLEX COMPONENT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12126">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 39 KDA SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="1">
        <location>
            <begin position="9"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="288" mass="30808" checksum="EB0FA77429E01C77" modified="2010-06-15" version="1">MATKTPRLVVCGGSGFLGSRICKSAVSRGWTVTSVSRTGEPDWNTTFGSPTPPEWSGKVTWARANLLSPSTYKEYLKDATAVVHSMGILLEADYKEALSGKENPITSICRAFDSAKGTSRNPLKENSSEKPQLTYETMNRDSAITLAEESIAAAPETLSSFVYISAASGSIVLPSRYLSTKREAEAAITALSTSKKPFRPILLRPGFLYDPTRPVTMPLAGLIGVASAVNSLFGGKLPLLGAAGYKPLKVGVVAEAAVEAIAHESVKGTVEVKDIEKLHSEAWRRDML</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBF2</accession>
    <name>D5GBF2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="18">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">GSTUM_00000442001</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="FN430093">
        <property type="protein sequence ID" value="CAZ81845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837654.1">
        <property type="nucleotide sequence ID" value="XM_002837608.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBF2"/>
    <dbReference type="FunCoup" id="D5GBF2">
        <property type="interactions" value="300"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GBF2"/>
    <dbReference type="GeneID" id="9184418"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000442001"/>
    <dbReference type="eggNOG" id="KOG0061">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_57_1_1"/>
    <dbReference type="InParanoid" id="D5GBF2"/>
    <dbReference type="OMA" id="FNCQLDA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03213">
        <property type="entry name" value="ABCG_EPDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000702">
        <property type="entry name" value="ABC transporter (Adp1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF2">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0732">Signal</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072048" evidence="15">
        <location>
            <begin position="23"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="300"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="809"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="842"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="886"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="922"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="952"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1041"/>
            <end position="1061"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="82"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="17">
        <location>
            <begin position="356"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="617"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="698"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="700"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="91"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="110"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAZ81845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1064" mass="117480" checksum="1F61FF845B263FDC" modified="2010-06-15" version="1">MLRYLGATLFFTSSLSTSLAEARNTSVTLDKDLLQTQHLWSLDNRPAECPPCFNCLLPAFNCKQFAECSSHDGKCECPPGFAGDNCLQPLCGSLAGDQHRPLKGDGPCECDEGWSGINCNVCQTDNACDALMPQGVDGVCYKQGIAVKQNFQMCDVRNRKILEMLKKNPQVTFSCDKGSAQCTFQFWVDQLQTSPDRNITDYECTNVKCECIPGRTLCGEGGTIDLSEFLTEGITGPATFSCDSSKPDDQCLFSEKAMNEVISSIFGDENILLNCDTGECLHRSEVPGYTRPIKTINKPLIAGVISGVALFIVTAILGLWYLSRRAGQRNGLGSIHLPDDDDEGTRLMTEHWPAALHFDKLSYSLNEKRILKDVAGAVNPGQVMAIMGPSGAGKTTFLDILACKNKRGVVGGNIYVNGSLVSDEEYREVIGFVDQEDTMMPTLTVYETILNSALLRLPREMNYQAKNRRVMEVMGQLGILGIKDQIIGTSEDNGLRGISGGEKRRVGIACELVTSPSVLFLDEPTSGLDSFNAYNVVECLVNLARNYKRTVVFTIHQPKSNIVALFDQLVLLAKGRCVYSGPFDKCQRYFDKLGYPCPQGFNIADYLVDLTMHAEQRRSTPREEPLIEASEAPIPVSPRPSPSVMSESASADCSPRKKFRKRGDSIRALQERQLFTRRRSEFASGGAEEVMMSDSLENTRAWRDSSEPRQSLEETEHLLPPVAQSGTDATDLDVLIAAYKDSDVAVGVRDEIENAARVTLNGAIDGNANGNADDHGVSGAMKGFRRVGWIGQFAILSRRTWKNLYRNPLLMLAHYAISILLGVLCGYLFFGVRDDISGFQNRMGLIFFVLALFGFSTLTSLNVFAAERTLFLRERANRYYTPVTYFAAKVTFDIVPLRVIPPIIMGAILYPMVGFVAEWPEFLKFMLVLILFNLAASAICLFIGIVFKDTSLANLVGSLVMLFSLLFAGLLLNHDSIPKGALWLQAVSIFHYGFEALIVNEVRYLSLYEHKFGLDIEVPGATILSTFGFDSLAYWKDVINLGIFAGSFIILAYAAMHLLLVEKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBH8</accession>
    <name>D5GBH8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00000505001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430096">
        <property type="protein sequence ID" value="CAZ81871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837680.1">
        <property type="nucleotide sequence ID" value="XM_002837634.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBH8"/>
    <dbReference type="STRING" id="656061.D5GBH8"/>
    <dbReference type="GeneID" id="9184444"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000505001"/>
    <dbReference type="eggNOG" id="ENOG502S8N7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112091_3_1_1"/>
    <dbReference type="InParanoid" id="D5GBH8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056121">
        <property type="entry name" value="DUF7704"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37019">
        <property type="entry name" value="CHROMOSOME 1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37019:SF2">
        <property type="entry name" value="EXPERA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24803">
        <property type="entry name" value="DUF7704"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7704" evidence="2">
        <location>
            <begin position="4"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ81871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="113" mass="12360" checksum="6E68E8FFC7C0390B" modified="2010-06-15" version="1">MATVSPNVKASILQLANCYLLLSMCSTAVLRSTTEESVAKRFLFALLLGDAGHVYLTYTAVGADYFFTPSQWNLLTHGNITFTIFLFLSRSIYLLGWGGSAGSQKPIKTKKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5GC21</accession>
    <name>D5GC21_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">phospholipase D</fullName>
            <ecNumber evidence="1">3.1.4.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00005776001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.4.4" evidence="1"/>
    <dbReference type="EMBL" id="FN430098">
        <property type="protein sequence ID" value="CAZ82064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837873.1">
        <property type="nucleotide sequence ID" value="XM_002837827.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GC21"/>
    <dbReference type="STRING" id="656061.D5GC21"/>
    <dbReference type="GeneID" id="9181333"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005776001"/>
    <dbReference type="eggNOG" id="KOG1329">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000690_2_2_1"/>
    <dbReference type="InParanoid" id="D5GC21"/>
    <dbReference type="OMA" id="INNAQHY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004630">
        <property type="term" value="F:phospholipase D activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009395">
        <property type="term" value="P:phospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09138">
        <property type="entry name" value="PLDc_vPLD1_2_yPLD_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09141">
        <property type="entry name" value="PLDc_vPLD1_2_yPLD_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025202">
        <property type="entry name" value="PLD-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015679">
        <property type="entry name" value="PLipase_D_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896">
        <property type="entry name" value="PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896:SF186">
        <property type="entry name" value="PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13091">
        <property type="entry name" value="PLDc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PLD phosphodiesterase" evidence="7">
        <location>
            <begin position="536"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="7">
        <location>
            <begin position="833"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="13"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="40"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="154"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="189"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="199"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="285"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="306"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ82064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1062" mass="118997" checksum="45C20DCCC6A3F356" modified="2010-06-15" version="1">MGKSSKYRSSSRSPERRDEDDYRYEKHSKKRYDGGSGREYSGDEKPGRVRDGEYYREKGHRKREKEEKKERKRREKEKEDSGEYGSPGRPRSRERSTQEQNYQPSPHGQHYGDQPGHGYQAPPHESGYPATGSEHPGHQPSSYGPGVYTSAGYQPPPPAQGYPPASPGYGGFQGPPAPPLYATGGFQGYPPPPGPPPTSQQYGGYSSGSAPYSYPPPPPQQEYGGYAQGGHGQSQYPAPPHQSPHQPLNHPAVSQGGPVYVSLPPPPKVTAGGATAGQHYKPQHHSAQQQQAYYGHDSGTVDYPTGGSTAVQFENTNPANPHSKPSAGSGGSAVSALETFTGALTSSLHSYARTLFPATSAHKPVAQYGAPGESGAPAQQTENRFDSFAREKTGNGVKWYVDGKDYMYAVSIAIENAHQSIWILDWWLSPELYLRRPPKLNEQYRIDVMLKAAAERGVKINVIVYKEVTQALTLSSHHTKTSLENLHPNITVFRHPDHLPDRPTIQSDIWNKVTTGTYQLGDGLKGLYGMKDGVTMYWAHHEKLCLVDGEIAFMGGLDLCFGRWDMNHHPIADAHPSDLRAIIFPGQDYNNSRFMDFHTVDHWEQNKVKREEHSRMGWSDVALSVIGPSVKDLQRHFIGRWNFIYYSDDYGYDEPRGGYEEEKAYSERRHDEVEGVRCQIVRSSSKWSHGLPATEHSIAHAYIEIISAATHFVYIENQFFITATDDKQNPILNKIGRAIVDRILRADREHQKFKMIIVIPAVPAFAGDLKDDGSLGIRAIMEFQYRSINNDRGYSIMEQIAKAGVDPKKYIRFYNLRSYDRINAGQLDAFVSEELYAHSKLLIADDRIVICGSANLNDRSQLGDHDSEIAIIIEDPTPLPSLMDNKPYKASHFAATLRRQIFRKHLGLIPAANLTTVDENMHPVPIPNIYDFDSQEDLMVTDPLSETFENFWNYTARANTAAFEKVFHVVPTDKVANWKEYDEYWGKHFRVPPGENQQPKIKWGHVVRDEFNPDPMVAVGEIKGVLATVRGHLVEMPLGFLREEDIAKEGLGLNAITESLYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCG3</accession>
    <name>D5GCG3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00005878001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430109">
        <property type="protein sequence ID" value="CAZ82206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838015.1">
        <property type="nucleotide sequence ID" value="XM_002837969.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCG3"/>
    <dbReference type="GeneID" id="9186388"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005878001"/>
    <dbReference type="eggNOG" id="ENOG502S61K">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_886205_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCG3"/>
    <dbReference type="OMA" id="SAERCEV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="265"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="279"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="314" mass="34512" checksum="F98A2AEDBF15AABB" modified="2010-06-15" version="1">MNPVSQSVPCLPTVFKLGLVTPDTPSFPLRGDGEEEAHDSKRLRSSQAQKEGTSHAMDSHIPTPTLTNSPGSPLEMEYSYEEFERYLPLSNFPTPPLSDQYSPELPAGLNFEDVLNPELYGPASYLCSMLPKNASREAPSTHLLQSILQRANVAMEVVGLASCILDCLSGQFVRRWRQEYCAAEGSAERCEVLAVAALCIALKFLEDTSLSSRMWARDICGDRFSPRMLSITERLILGDVGFSIAGICTAELIQYSINEMKRHSKTAPKAKRPSCPTAAESSPSKQFTKTTFPRLSELDSFVSDTLVDLPVSIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCN1</accession>
    <name>D5GCN1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00000763001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430117">
        <property type="protein sequence ID" value="CAZ82274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838083.1">
        <property type="nucleotide sequence ID" value="XM_002838037.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCN1"/>
    <dbReference type="STRING" id="656061.D5GCN1"/>
    <dbReference type="GeneID" id="9187894"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000763001"/>
    <dbReference type="eggNOG" id="KOG0159">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022195_1_0_1"/>
    <dbReference type="InParanoid" id="D5GCN1"/>
    <dbReference type="OMA" id="WVEVSIC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11041">
        <property type="entry name" value="CYP503A1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46206">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0349">Heme</keyword>
    <keyword evidence="5 6 7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="179"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="6">
        <location>
            <position position="334"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR602403-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ82274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="397" mass="45098" checksum="739B40F75C7BC49C" modified="2010-06-15" version="1">MLINLTNRQHVRRPAGRDTPRLGFRASGICYQWLVFFRTHCRFSCEWLSVDLFPTILRIVARVSARSFIGLPVCRNEDWLKLAITFTEDLIATASNLGTISKLFRPFYAYMWNSAKTIKSHKRKAQTLLAPTIQKRFKEQRLAEENGTVYQGPNDLLQWLIDRVEPHHKTVDALSELQLLIGLASVFATAGTLVGMIFDLAEYRDCIQPMREEMEAAISENSGALDRGVLRKMRKTDSFLRESMRMGTLLLSFNRMVMKNVTLSDGTYLPKGTLVAAPALMFSSDPDFVEDPETFDGFRWYKKTLEAKDGVQMSNGSATTSSNNLAFGHGKHACPGRYFAVEEIKTILTFILLQYDIKYPEGQSRPPNIRIGEIASPNRTQKIMFKKLPGPKTFSFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCV4</accession>
    <name>D5GCV4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00000791001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SVF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430124">
        <property type="protein sequence ID" value="CAZ82347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838156.1">
        <property type="nucleotide sequence ID" value="XM_002838110.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCV4"/>
    <dbReference type="FunCoup" id="D5GCV4">
        <property type="interactions" value="96"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GCV4"/>
    <dbReference type="GeneID" id="9183810"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000791001"/>
    <dbReference type="eggNOG" id="ENOG502QQY3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030205_2_0_1"/>
    <dbReference type="InParanoid" id="D5GCV4"/>
    <dbReference type="OMA" id="AFWPRCV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051385">
        <property type="entry name" value="Ceramide-binding_SVF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033394">
        <property type="entry name" value="Svf1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013931">
        <property type="entry name" value="Svf1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47107:SF1">
        <property type="entry name" value="CERAMIDE-BINDING PROTEIN SVF1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47107">
        <property type="entry name" value="SVF1-LIKE PROTEIN YDR222W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08622">
        <property type="entry name" value="Svf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17187">
        <property type="entry name" value="Svf1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159245">
        <property type="entry name" value="AttH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Svf1-like N-terminal" evidence="4">
        <location>
            <begin position="59"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Svf1-like C-terminal" evidence="5">
        <location>
            <begin position="222"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF17187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ82347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="383" mass="42032" checksum="3C3B82E42AA58DFA" modified="2010-06-15" version="1">MLSWVKSAAQSTLATVAGTAEPIYGPEAFHSVVKQESLNPSNEIAKEDMKWNVLETTSVETQTFYLAADSGHHGLVQVIYSNVANLHFTVQLSTKIFHPDGRIVWSSKPVTNYGFDEGNYSFYADDMAITMSEDGNSYSIKSMVDSELSIDLTCTRSGPGFKIGRDGKSLYGTDPVEPWGAIRHIFWPRNKLTGFMVVAENGEPVKIDFSGSAMLVMALQAMKPHHAAAKWNFVNFQGPTMCAVMMEFTTPPSYGSSKVNVSGIVTDGEIIAASTCGTVEHLKTQLDEEAGWPEPTDAKFVWKSSKHGKDVEAVIEGPLGDRIDRVDVMAEVPAFIKKIASSAAGTKPYIYQYSKQMTLKVKIGDRVIEEEGKFFGEATYICE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GD24</accession>
    <name>D5GD24_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00000912001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Component of the NuA4 histone acetyltransferase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430137">
        <property type="protein sequence ID" value="CAZ82417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838226.1">
        <property type="nucleotide sequence ID" value="XM_002838180.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GD24"/>
    <dbReference type="STRING" id="656061.D5GD24"/>
    <dbReference type="GeneID" id="9183889"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000912001"/>
    <dbReference type="eggNOG" id="KOG1911">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045874_0_1_1"/>
    <dbReference type="InParanoid" id="D5GD24"/>
    <dbReference type="OMA" id="KCPLKML"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000792">
        <property type="term" value="C:heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00024">
        <property type="entry name" value="CD_CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18657">
        <property type="entry name" value="CSD_Swi6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008251">
        <property type="entry name" value="Chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023779">
        <property type="entry name" value="Chromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051219">
        <property type="entry name" value="Heterochromatin_chromo-domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22812">
        <property type="entry name" value="CHROMOBOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01393">
        <property type="entry name" value="Chromo_shadow"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00300">
        <property type="entry name" value="ChSh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00598">
        <property type="entry name" value="CHROMO_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromo" evidence="5">
        <location>
            <begin position="49"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="105"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="137"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ82417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="225" mass="24927" checksum="C1E69CF95891878D" modified="2010-06-15" version="1">MPPQIVTSDSDSDGDIIAAKATTNDVDVASVASGDDDVAADVPEDEEEYIVEGIKSHRYDGKQLFLLVKWKGYERKSDMTWEPEENCEGAKEILQQYYTSIGGRPTYESSPALKGKRSRTSLGSTPTASKAVKRAKTSTPAATNKNGNESSPADGDSWTPPKGSWEEEITCIDTIEKTEKGLVCYVQWNNGRKSQHEISTVYRKCPQTMLRFYEQHLVFKESGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5GD47</accession>
    <name>D5GD47_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00000972001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFIIA subunit 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430140">
        <property type="protein sequence ID" value="CAZ82440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838249.1">
        <property type="nucleotide sequence ID" value="XM_002838203.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GD47"/>
    <dbReference type="FunCoup" id="D5GD47">
        <property type="interactions" value="451"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GD47"/>
    <dbReference type="GeneID" id="9183904"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000972001"/>
    <dbReference type="eggNOG" id="KOG2652">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030027_4_1_1"/>
    <dbReference type="InParanoid" id="D5GD47"/>
    <dbReference type="OMA" id="EVCDASQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005672">
        <property type="term" value="C:transcription factor TFIIA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07976">
        <property type="entry name" value="TFIIA_alpha_beta_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.18.10:FF:000006">
        <property type="entry name" value="Transcription factor TFIIA complex subunit Toa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.18.10">
        <property type="entry name" value="Transcription factor IIA (TFIIA), beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004855">
        <property type="entry name" value="TFIIA_asu/bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009088">
        <property type="entry name" value="TFIIA_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12694">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIA SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12694:SF8">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIA SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03153">
        <property type="entry name" value="TFIIA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01371">
        <property type="entry name" value="TFIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47396">
        <property type="entry name" value="Transcription factor IIA (TFIIA), alpha-helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50784">
        <property type="entry name" value="Transcription factor IIA (TFIIA), beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="57"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="155"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="262"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="79"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="90"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="172"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="296"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ82440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="373" mass="40747" checksum="89BFC703CCE0AC05" modified="2010-06-15" version="1">MSNQVVGEIYQRIIDDVINNSIIDFEEAGVNASTLQELKQVWQSKLTDLNVATFPWDPPSETVQASGSAPAPNSPTKVQNNNSQQQQQNTIKQEPGAQNPNPQTVNVGVRIKPEPGMEPQGVYAPPAQPTGNPTHNPALAEARARQNLAQKFGNTPQLARPAGGLVLPGLATNPQQRPNGQQSGIPQHDGGGEETIPIEGYGSISRSEADKILLRRISDAERESARATDELVKALMNLGHSSKSSKAKGRRRAQDPYEEALDPFNHYPHAAPVPTVLDVSNTPSPRPAPMGQVDGESGDEEVVDEDAINSDLDDPEDDEIDGEDDDEVPQIMLCMYDKVQRTKNKWKCVLKDGVLTINQTEYVFHKANGEYEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDA6</accession>
    <name>D5GDA6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00006124001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430142">
        <property type="protein sequence ID" value="CAZ82499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838308.1">
        <property type="nucleotide sequence ID" value="XM_002838262.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDA6"/>
    <dbReference type="GeneID" id="9183963"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006124001"/>
    <dbReference type="HOGENOM" id="CLU_518944_0_0_1"/>
    <dbReference type="InParanoid" id="D5GDA6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="353"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="473"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="88"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="372"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="473"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="525" mass="57472" checksum="F3DD0F9EC63A77CC" modified="2010-06-15" version="1">MNRIDSTYGQYRRTRVEGILSQGVPSLDKTAGGTGYHDLGGTIPSDFSLSNNTPQISTPYESIINEEIYLRDPETFTNPTYLTPGPYTATKSTASNSNTLTRTPVPAVGAHVEHGKHGTSTSSKRGRETGGKGGSQQGPAHKRARRNRAPSVRSSNVGAGDEDEEDEEDEDEEDEESDEDDAPIMSSGPKEPNVLLCPMPSCRGKGKQWDNWNNLIQHLQNTHIKGFSVCERGCGRRFGLSSQYTRHVFKRKKECKPLPLSGPPRRIPPLALDFNHAVAKVKGQNYARLKQVIEAHNYYDLEDPNHPEHRVEPSCYSTSSQNLHQYNPQGQLMPDWVEQIIRLLTGHQRVCQMHPQTDPPPSDPVRADGRLNGSSGLVENQHIPQNSTDPTIPLQLNQPAARRPHVYGTQPSAIAACIPDFQNGSSSSISGGYTPSFTRRLGQDTQGGGFARFGTGDYPQVVGSDYLSQCPTHTGSYIGNNTRANRGAPPSEPDHSPLPSDIFPFGIGVPYVDGTSDAPHFNMES</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDB2</accession>
    <name>D5GDB2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00006133001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430142">
        <property type="protein sequence ID" value="CAZ82505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838314.1">
        <property type="nucleotide sequence ID" value="XM_002838268.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDB2"/>
    <dbReference type="STRING" id="656061.D5GDB2"/>
    <dbReference type="GeneID" id="9183969"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006133001"/>
    <dbReference type="HOGENOM" id="CLU_555657_0_0_1"/>
    <dbReference type="InParanoid" id="D5GDB2"/>
    <dbReference type="OMA" id="QNTHADM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016071">
        <property type="term" value="P:mRNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028322">
        <property type="entry name" value="PNRC-like_rgn"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15365">
        <property type="entry name" value="PNRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="183"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="260"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="284"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="332"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="419"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="23"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="231"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="295"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="424"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="485" mass="52029" checksum="CFB90809DA05413B" modified="2010-06-15" version="1">MTATNGSPSPKPRPGKHHRHTRSSSSLTPSGPKNHHSLNQGSQVPFNTHYIPFNNHHHNNNPAVRSAGAVLTPPQTPKAQGFIASMETSGKKKKKTRGRQKDSVPATKPVPLPFSPVGTNESVKTQSENIAEPISLGQMTPPKLSGTPMKAYAGPLFHSSPNPSALPVPKFFSKSVPVTRAGNGLQAMVESEDSEDNSTTSSVEQTGESHLQKLFRVDKEEKERLKMKRQSSGSSSSTEGSIGTPFDCSEFNGSSVFEMVRSAPRTPRPVDGIFSIDMDKPTSPLPTRLFHRTHSSPFNHTLSATQKNQTVYTPSKGDDLKRTADLLKSHLLSPLASPNHDEPSSPVPAPRSQRPNLNAGPPLTPTRNNQASPVQGFVGIPSYSLDRAPRTSVNDLFGSVVRQAGPPQSPLALRASPFCRDAPLNSSSPQRNSQPKKSALASKLETARGSDTPSKSVAEMENDLRRALNLDSYVSNAALGGGVLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDK0</accession>
    <name>D5GDK0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00001055001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430147">
        <property type="protein sequence ID" value="CAZ82593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838402.1">
        <property type="nucleotide sequence ID" value="XM_002838356.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDK0"/>
    <dbReference type="STRING" id="656061.D5GDK0"/>
    <dbReference type="GeneID" id="9181598"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001055001"/>
    <dbReference type="eggNOG" id="ENOG502QX51">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_457231_0_0_1"/>
    <dbReference type="InParanoid" id="D5GDK0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013951">
        <property type="entry name" value="Rxt3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08642">
        <property type="entry name" value="Rxt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="223"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="559"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="148"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="236"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="597" mass="64653" checksum="0B7EDDEF32F43270" modified="2010-06-15" version="1">MASRSFYGGEPRQVGHQPQTSTSHLSYPMTITSRMPSNGPQNHVEMSGAPLSMSGMPNSAAISLPQTSTAGAGGLGPTPAPLPTATATGRGDFGSFTGYTTPMPPSPFAMPASLPTVLPPVGDGTRDSRRQNLQNGRDEPTRAEDALRGGLSRALSSGLSSIVGEEERRRRVEKERDGNARVSGPNAGNQSPNKRLSSGFSIGMGMPNMAKSWDDSIQKAFPEANREGGKPSNALGKRERDEEEAGKLEKKKKHHHHHSLHAVHHHRHHHKVDDEPAGSGLEIPLARAGARSKSPNSMAMAHPHHVHQVTVHHHHPAAVSPAPPPQRVKTSLLIDSSKVLASLASKPRQYLGSTIYQPTPTPKQGLSVTQPLLPRFEGRENCIFQVRIPRRFLSAEQRRDVHRRRCVWGTEVYTDDSDVLGVLVHLGKVPGVLPEGATPDLVAETGCRKTTSRGDITNMLSTIPSPVRKKGKGAHAADEGPTIQPGKDLIVNLLILPTLQKYTGSVRNALKSRSWNTIHDGMSYSIWDMEWVEAGEAEARGGGSRKRRLDEREWVRRWGELPPSRGGGGVGKVGWNKGRWSESKTGMRKGNVTEVGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDM5</accession>
    <name>D5GDM5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00001121001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430152">
        <property type="protein sequence ID" value="CAZ82618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838427.1">
        <property type="nucleotide sequence ID" value="XM_002838381.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDM5"/>
    <dbReference type="STRING" id="656061.D5GDM5"/>
    <dbReference type="GeneID" id="9181629"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001121001"/>
    <dbReference type="eggNOG" id="KOG4120">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042829_1_0_1"/>
    <dbReference type="InParanoid" id="D5GDM5"/>
    <dbReference type="OMA" id="MCVVGKS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008263">
        <property type="term" value="F:pyrimidine-specific mismatch base pair DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004844">
        <property type="term" value="F:uracil DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006285">
        <property type="term" value="P:base-excision repair, AP site formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10028">
        <property type="entry name" value="UDG-F2_TDG_MUG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.470.10:FF:000010">
        <property type="entry name" value="G/U mismatch-specific DNA glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.470.10">
        <property type="entry name" value="Uracil-DNA glycosylase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015637">
        <property type="entry name" value="MUG/TDG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005122">
        <property type="entry name" value="Uracil-DNA_glycosylase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12159">
        <property type="entry name" value="G/T AND G/U MISMATCH-SPECIFIC DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12159:SF9">
        <property type="entry name" value="G_T MISMATCH-SPECIFIC THYMINE DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03167">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="Uracil-DNA glycosylase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0234">DNA repair</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Uracil-DNA glycosylase-like" evidence="5">
        <location>
            <begin position="111"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="27"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="297"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="68"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="78"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ82618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="317" mass="34992" checksum="78EAEF4A2DC06D19" modified="2010-06-15" version="1">MPPIRREKTNSTATKAFQERLESFAFSSVSTRTRAANRAASKSNTPPATDQGPEEAPSANLDSTSKMVATRTTVRAVTKSRAKPASRRGKQKSSYAPPEKYAHLGGVQDAFAPNLLSVFIGLNPGITTATVGHAYSAPSNHFWKLLHSSGLTPDRVCPSTEDIHLPRKYSLGNTNLVARATLNQAELSREEMDDRVELTEDKIRRWKPESVCIVGKGIWESIYRAKHNGAKIPKGKFVWGWQSDEDRFGVDVEEGYPGARVFVVPSTSGLVAGYSMQFKKDIWKVLGDWVQMRRKERGETAPRTDNLVEGESSGVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDU7</accession>
    <name>D5GDU7_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ubiquitin thioesterase OTU</fullName>
            <ecNumber evidence="11">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00006280001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Hydrolase that can remove conjugated ubiquitin from proteins and may therefore play an important regulatory role at the level of protein turnover by preventing degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 16">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="11"/>
    <dbReference type="EMBL" id="FN430153">
        <property type="protein sequence ID" value="CAZ82690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838499.1">
        <property type="nucleotide sequence ID" value="XM_002838453.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDU7"/>
    <dbReference type="FunCoup" id="D5GDU7">
        <property type="interactions" value="659"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GDU7"/>
    <dbReference type="MEROPS" id="C85.007"/>
    <dbReference type="GeneID" id="9184485"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006280001"/>
    <dbReference type="eggNOG" id="KOG3288">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049327_0_0_1"/>
    <dbReference type="InParanoid" id="D5GDU7"/>
    <dbReference type="OMA" id="TRCILVY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22745">
        <property type="entry name" value="OTU_OTU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.80:FF:000016">
        <property type="entry name" value="Putative ubiquitin thioesterase otu1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048857">
        <property type="entry name" value="OTU1_Ubl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003323">
        <property type="entry name" value="OTU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057766">
        <property type="entry name" value="Znf-C2H2_OTU1-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13312">
        <property type="entry name" value="HIV-INDUCED PROTEIN-7-LIKE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13312:SF0">
        <property type="entry name" value="UBIQUITIN THIOESTERASE OTU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02338">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21403">
        <property type="entry name" value="OTU1_UBXL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24560">
        <property type="entry name" value="zf-C2H2_OTU1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50802">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 11" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="7 11" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="OTU" evidence="13">
        <location>
            <begin position="128"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="85"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="85"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ82690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="326" mass="35823" checksum="ABE25DDA17F1C2FE" modified="2010-06-15" version="1">MRIKIRAPNGSQVLALSPETTISSFLSEIRSKTSLSGDVEIKYGYPPKSLHLSEYPSSTLLRDIPTKLEGEQLIISETKSSAPFSFSGGAQDSTSQPDAPLTLNRSLKPKINKDDPPDVPLKSGRGTMVLRVMEDDNSCLFSAISYVATGSLYSAAELRQLIATTIQENPDIYSEAVLEQKPDGYCEWIQMESSWGGGIELGIFANFFDLEICTIDVSTNEVIRFNEGKANRAVVVYSGIHYDTIVLSPAGSRTNDPSKDKRIFSSSDDEELEGALELCRELKKRKYFTDTKSFSLKCNICKTGLKGQKAAVAHAKSTGHMDFGEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GE66</accession>
    <name>D5GE66_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00006400001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430164">
        <property type="protein sequence ID" value="CAZ82809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838618.1">
        <property type="nucleotide sequence ID" value="XM_002838572.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GE66"/>
    <dbReference type="STRING" id="656061.D5GE66"/>
    <dbReference type="GeneID" id="9183141"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006400001"/>
    <dbReference type="eggNOG" id="KOG4727">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067237_2_0_1"/>
    <dbReference type="InParanoid" id="D5GE66"/>
    <dbReference type="OMA" id="VDHRRKW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040107">
        <property type="entry name" value="Snu23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022755">
        <property type="entry name" value="Znf_C2H2_jaz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45986">
        <property type="entry name" value="ZINC FINGER MATRIN-TYPE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45986:SF1">
        <property type="entry name" value="ZINC FINGER MATRIN-TYPE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12171">
        <property type="entry name" value="zf-C2H2_jaz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="104"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="176"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="23"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="185"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="195"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ82809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="247" mass="27692" checksum="A2C1377203CD1DA7" modified="2010-06-15" version="1">MSGDKKNAYGTETGGDTNFRRTWNREEWTAKAQERESQDRAASKLHQEAKLAGKKFHRPPTPPDASSTSARASRLNLEENLNKTTLVPAGAGVGKRGRGAGFYCEACDLTFKDSLQWVDHLNSKQHLHAVGERDEVVVATLEMVVERLAWLKRRREEEGRDGEEVDLARRLADRARAEEEERVQRREKRKVARRERKERERKLGKEGDVEGGDGDGKGGGEEEDPDAVAMARMMGFAGGFGTTKKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEG8</accession>
    <name>D5GEG8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="12">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00006478001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="FN430175">
        <property type="protein sequence ID" value="CAZ82911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838720.1">
        <property type="nucleotide sequence ID" value="XM_002838674.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5GEG8">
        <property type="interactions" value="580"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GEG8"/>
    <dbReference type="GeneID" id="9181542"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006478001"/>
    <dbReference type="eggNOG" id="KOG0160">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000192_9_0_1"/>
    <dbReference type="InParanoid" id="D5GEG8"/>
    <dbReference type="OMA" id="EIMFDDR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15480">
        <property type="entry name" value="fMyo2p_CBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.240.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002710">
        <property type="entry name" value="Dilute_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046943">
        <property type="entry name" value="Fungal_Myo2/2A_CBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF706">
        <property type="entry name" value="DILUTE CLASS UNCONVENTIONAL MYOSIN, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01843">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM01132">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51126">
        <property type="entry name" value="DILUTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4 7" id="KW-0518">Myosin</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myosin motor" evidence="11">
        <location>
            <begin position="1"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="Dilute" evidence="10">
        <location>
            <begin position="993"/>
            <end position="1262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="401"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="7">
        <location>
            <begin position="439"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1322"/>
            <end position="1348"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="689"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="412"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ82911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1348" mass="154726" checksum="336901232441D87C" modified="2010-06-15" version="1">MEAFGNAKTTRNDNSSRFGKYIEIMFNKEIDIVGAKIRTYLLERSRLVYQPATERNYHIFYQLIAGASDEEKKELGLLPVDYYDYLNQGGDPIIPNVDDAAEFKLTREALGTIGVSEVTMARIWKVLAALLHLGNTKIEASRNSSVLLTTDPALVKACELLGVNAEEFAKWTTKKQLVMRNEKTISPLSQKQALVVRDSISKFIYSSLFDWLVEVINNGLAAEGVREKVKSFIGVLDIYGFEHFKRNSFEQFCINYANEKLQQEFNQHVFKLEQEEYAREQINWAYIEFSDNRPCIELIEAKLGILSLLDEESRLPAGADETFVEKLHQNFAAGRPKGFPYQKPRFGKTSFTVCHYAVDVTYDSDGFIEKNRDTVPDEHLELLKASTNPFLTEVIQSATSLRDKENAPVPKTTPGPAARRAGGMANRKPTLGGIFKSSLIDLMGTIGSTNVHYIRCIKPNEAKVAWKFEGPMVLSQLQACGVLETVKISCAGYPTRWTYEEFASRYYMLVHSSNWKDKFQLGMSKIFFRAGMACIPTALAYLENLRTTRLNKCAILIQKNLKKKYYRRKYLEARESILKLQAWARGCLARKEAQAMRVVKAAVTIQRVWRGSKERKAFNRIRNSVILAQAAAKGYLCRKSITDRMLGNAARYIQKAWRSRRFLQAWRTYRKRCVYIQSVWRGKIARRQYRKMREEARDLKQISYKLENKVVELTQTVGKLKQDNKALLEKVESFDNQVRNWRTKHATLENRNKELQIEANQAGIASARLSQMDEEMKSLQRQFEESTANMKRLQEEGKSLRAALSKRTSELDILKSRDQVHEDEKMSLRQQIQALEDQLQNAGPPVEENGHVEKVPAGLANGIINFVSNKKPKRRSAEVNRFENDGMYRSSYQARPVSMMPTTAPIRNRDLDALTFSPVVDNTEYELQRLLEEDEALSEEVAMGLIRNLKIPSPSSQNTLVEKEVLFPAYLINVVTSEMWNNGFVKESERFLANVMQSIQHEVMAHNGDDAVNQGAFWLSNVHEMLSFVFLAEDWYEMQKKDDYEYDRLLEIVKHDLESLEFNIYHTWMKVLKRKLHKMIVPAIIESQSLPGFITNDGNRFLNKFLTTSSAPQYSMDDLLTLLNKVYKALRGYFIEESIITQAVTELLKLVGVTAFNDLLLRRNFLSWKRGLQINYNITRIEEWCKSHDMPEGTLQLEHLMQATKLLQLKKATLNDIEIIQDICWMLSPNQIQKLLNQYLVADYEQPINGEIMKAVASRVTEKSDTLLLQAVDMDDSGPYEIAEPRAITALETYIPSYLQTTRLRRLAEIVSAQAQARVEAERVREGVDDSDGDTKTPIPTELEPVHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEH1</accession>
    <name>D5GEH1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00006482001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430175">
        <property type="protein sequence ID" value="CAZ82914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838723.1">
        <property type="nucleotide sequence ID" value="XM_002838677.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEH1"/>
    <dbReference type="GeneID" id="9181545"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006482001"/>
    <dbReference type="HOGENOM" id="CLU_2308120_0_0_1"/>
    <dbReference type="InParanoid" id="D5GEH1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072305" evidence="1">
        <location>
            <begin position="23"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="100" mass="10885" checksum="A098DD058F205851" modified="2010-06-15" version="1">MLFRGWWVKACFLLTGTRANFATRPLYSTLGRVIMTPVDTGAYPSSLTPELHRACGSRRGGRGSLVAEGHGPTGCPTLPVYHSIIARMILVLSRILVSNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5GET4</accession>
    <name>D5GET4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00001369001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430189">
        <property type="protein sequence ID" value="CAZ83027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838836.1">
        <property type="nucleotide sequence ID" value="XM_002838790.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GET4"/>
    <dbReference type="STRING" id="656061.D5GET4"/>
    <dbReference type="GeneID" id="9184939"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001369001"/>
    <dbReference type="eggNOG" id="KOG0393">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_21_2_1"/>
    <dbReference type="InParanoid" id="D5GET4"/>
    <dbReference type="OMA" id="YEKWIQE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000983">
        <property type="entry name" value="Rho family GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ83027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="211" mass="23632" checksum="978ACE66242D284D" modified="2010-06-15" version="1">MGRSQRRSRQQGSGEGALRRKLVIVGDGACGKTCLLMRFSKNTFDPDGYIPTVFDNYVADVHLPDGRNVELALWDTAGQEDYDRLRPLSYPDSHVILICISIDSPDSLQNVYEKWIQEVRHFCPGLPVVLVGCKKDLRNDERTKRRLWGEEGGKMPLTEEDGRNAVKQLGLTAYIECSAKTNDNVKTVFEEATRVALNSPKRTSKGGCTIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5GF67</accession>
    <name>D5GF67_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00006747001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430208">
        <property type="protein sequence ID" value="CAZ83160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838969.1">
        <property type="nucleotide sequence ID" value="XM_002838923.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GF67"/>
    <dbReference type="FunCoup" id="D5GF67">
        <property type="interactions" value="527"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GF67"/>
    <dbReference type="GeneID" id="9188021"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006747001"/>
    <dbReference type="eggNOG" id="KOG1944">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049109_8_1_1"/>
    <dbReference type="InParanoid" id="D5GF67"/>
    <dbReference type="OMA" id="WYQSKLA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006067">
        <property type="term" value="P:ethanol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF17">
        <property type="entry name" value="PROTEIN MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04117">
        <property type="entry name" value="Mpv17_PMP22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ83160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="200" mass="22690" checksum="B80A9EF42235E256" modified="2010-06-15" version="1">MLTWYRTALTKRPVLTQCLSTSFLFAAGDVIAQQAIEQRRSDGLRTHNPYRTLRMAIYGGSIFGPLVVNWYKFLQTAVRIPASPSLEIVSRVALDQTLFTPVHLTLFFSSMATMEGIMGDDGRELGTEERVRGKLRDNWLQGLRANWTVWPGVQLVNFRFVPLEHRVLVVNLVSLGWNSYLSYLNQQGKGREGETGKEVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5GF90</accession>
    <name>D5GF90_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00006784001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430208">
        <property type="protein sequence ID" value="CAZ83183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838992.1">
        <property type="nucleotide sequence ID" value="XM_002838946.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GF90"/>
    <dbReference type="GeneID" id="9188039"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006784001"/>
    <dbReference type="eggNOG" id="KOG0158">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_14_0_1"/>
    <dbReference type="InParanoid" id="D5GF90"/>
    <dbReference type="OMA" id="IRFAHIN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11060">
        <property type="entry name" value="CYP57A1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050121">
        <property type="entry name" value="Cytochrome_P450_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305:SF232">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="3 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="460"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602403-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ83183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="514" mass="57223" checksum="34AB4D227A78ECB8" modified="2010-06-15" version="1">MPSLLLMSLAPSLKTLVVLEFTQVLPLALLALVASCAYSRYASPLKNVPGPALASFSRIWLNVGLVARGNLHGGIMDAHRKYGPVVRVGPNKVYFSDPALIPEIYGVGTGYRKSDLYMMSDPEPGHGSTFSERSAEKHAVLRRRVGPAYSNSSVLQMESYVDALVSAWISALSSEFTVAKTSLKSCDFASWSQFLTYDVISELAFGKAFGFIATRSDYKQYIASFQQALPMIVTMSYFEELVKFMTLGWVRKFTTPSIKDETGYGNLTKTATDLVRERWAKGGVGERRDMLQRFYEEGMTEKEAMIDSTLILVAGSDSTATTIRAAVMFICSDPSVYRTLKSELAANIPRTSPPSVVSFSVARSLPYLSACIKEALRLFPPGAGAMERVVPVGGATLSGYFIPENTVVCLHPWPVCRDKVFGEDVDSFRPERWLEEKDEKRLVKMEKTLDFVFGAGPHYCLGRQIAYLELYKSIAELFLTFDIGLSNPMRPWKSREYGFFLQRDMMVKIKKVDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5GF95</accession>
    <name>D5GF95_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Mediator of RNA polymerase II transcription subunit 14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Mediator complex subunit 14</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00006790001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Mediator complex subunit 14 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430208">
        <property type="protein sequence ID" value="CAZ83188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838997.1">
        <property type="nucleotide sequence ID" value="XM_002838951.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GF95"/>
    <dbReference type="STRING" id="656061.D5GF95"/>
    <dbReference type="GeneID" id="9188044"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006790001"/>
    <dbReference type="eggNOG" id="KOG1875">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003573_1_0_1"/>
    <dbReference type="InParanoid" id="D5GF95"/>
    <dbReference type="OMA" id="ITQGYIP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055122">
        <property type="entry name" value="Med14_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013947">
        <property type="entry name" value="Mediator_Med14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12809">
        <property type="entry name" value="MEDIATOR COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12809:SF2">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08638">
        <property type="entry name" value="Med14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26204">
        <property type="entry name" value="Med14_fung"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0010">Activator</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Mediator complex subunit MED14 N-terminal" evidence="11">
        <location>
            <begin position="50"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ83188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="930" mass="104815" checksum="F4FF3636066B3F95" modified="2010-06-15" version="1">MPQAMTPGDLVDASRHDFQHGTPNHSGSAPGKQAELVVRNPRLPHITQGFIELRLLVSRLVQDSHNRLQGMIGLLAEGGRSDGDKKARIIEHMLERRKQFIKLLVLTMWAKNAQAVSEVIDLKVFLDTRQEIFHRVVWNLYEVRRKMSYARIPNPDLDTAVRVLSTGRANTAMTRRYVPPKKLTSGETLKILRDLNTLLSLRLALHEKLPPYFRDYTIASGRVTFTVADEFEADLSIGDEDPTSQLYLVDFRFIFKPSASTLPAGRIRDEVERTGNKILEQKGLEGIYDYLHGFVMTDKICTLARQFQIMLTGRWTENLVVEMRKRTLVVQYWVSRVTEGATKSWIEVGIKRGTGGRPSHVGVRWIRDGKAVQDQEVPLDVAVLSAENLIKTVVAMHTKHILTSIRGRLAHSPVFPLSAMVLTTHSTDSFNSSLKLQLTPSRPIKFLIEPITGRFALHPLTMVSTHVEAAMNNSPTGVEEVIIRLKFVLTQEEVETRARSMGWEVLKVLNIRKEELKGFFPNSTKYITYLRRKGWGKEWVVVMVLAETGQTWWVAKVYEAPSQWSMSMALEIPVQGEANATYGFLGNLEKISSAMITLYVNQSVLVEKGVQHKLQPSKVADPKLTIPDLYIRFSSLIRANWGIDVLRLTFQGLGTEGKCIVTVLGRTKEPMTQLGSTNISAADSDVSFHPQSGSYAIRFNINVGESVIEPLTEKLHRIERLIRFVAVIRRFKLPCLDVSLGRIAFTYDTESNSSAEVSFSGDREMKLHLPPRSPHIRIKGFLEHRLNVSGLETVVMALTVTLPLLLAFERIEAAVGDRSDGHVFVLSRGVDWFRIYYRRLGVVLDWQLQCRKSVLHWFVRDAASSNPESEAEIVGGEGRRKAEELKGIWSGEVEGDWEPLKSGAAAGLREVGALAWKIHELIYAGRRAIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFH1</accession>
    <name>D5GFH1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00006891001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430208">
        <property type="protein sequence ID" value="CAZ83264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839073.1">
        <property type="nucleotide sequence ID" value="XM_002839027.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFH1"/>
    <dbReference type="GeneID" id="9188212"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006891001"/>
    <dbReference type="eggNOG" id="KOG2408">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002329_1_0_1"/>
    <dbReference type="InParanoid" id="D5GFH1"/>
    <dbReference type="OMA" id="YEIMEFF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20612">
        <property type="entry name" value="CYP_LDS-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09817">
        <property type="entry name" value="linoleate_diol_synthase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.640.10">
        <property type="entry name" value="Haem peroxidase domain superfamily, animal type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019791">
        <property type="entry name" value="Haem_peroxidase_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037120">
        <property type="entry name" value="Haem_peroxidase_sf_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050783">
        <property type="entry name" value="Oxylipin_biosynth_metab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034812">
        <property type="entry name" value="Ppo-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11903:SF13">
        <property type="entry name" value="LINOLEATE 10R-LIPOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11903">
        <property type="entry name" value="PROSTAGLANDIN G/H SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03098">
        <property type="entry name" value="An_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00457">
        <property type="entry name" value="ANPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50292">
        <property type="entry name" value="PEROXIDASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="5" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="373"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR619791-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ83264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1079" mass="120668" checksum="75E4D9E9D9542926" modified="2010-06-15" version="1">MSFLGLFGGGSSANPNSEYGDESAATKEITYTGIAEDIKASGGKIPEDLKLLLETGAQKVSKGPVDDKQLVMERLIGLVASLPQNSANRKKLTSTIIDTLWDSLQHPPLSYVGDKYQYRQADGSYNNILYPDLGKAGTEYARTIRQDKKLYGAKPDAGLLFDLLMARGDNFKQNQAGISSVLFYHASIIIHDIFHTNRKDFSKSDASSYLDLAPLYGSNQEEQNLIRTMQDGLIKPDTFSDKRLLGLPPGICVLLVMYSRFHNYAAKTIKAINENGRFSLPASHATASPEDQAKNLAKLDNNLFQTARLITNGLYVNISLHDYIRGIANLNHSESTWTLDPRVEIDKSFDGEGTPRGVGNQVSCEFNLLYRFHSAVSKRDDAWTKDFFGKIFPGQDPASIGISQLLQGLIVFEKSISEDPAKRTFGGLKRTGADGSGAFNDDELVKILKESIEDPAGAFGANTVPEILKPVEVLGILQARKWQVASLNEFRAFFNLKKHKTFEDINPDPYVANTLRKLYDHPDMVEMYPGMFLEDTKPRMDPGMGLCAPYTVTRAVFSDAVTLVRSDRHLTLDYTPANLTNWGITEVAQDYDTLGGAKMFHLILNAFPSYFKYNSVYAMQPFYTPTESRKIFDKFGKSYLYSFDPPAKTASPIPILTHAGLKRVLNDQKNFKVPWGEAMEALNNEHDFMLAGDLSSHTQQRNLVGDAIYDVTGSRKQFKDYTEEITLKLLKREVYQLGKDPFNQVDIVRDIGNLAALHFAASLLYLPLKSDENPNGQYSEQELYKTLTDLTWFVFSDSDPTKSWEHRREAKKSIDKLGEIMVEEVKKFKTPIGIWDKLTGGTGVRPPPPSLKDYGSNLVKRLLGSGKSAEDVAWMLLWSGCAFVANSACAFAQLIDFYLQDDNRKHWAEIQRLSSLNTPEADKLLTKYTLEGTRLSNSLGIFRTVDPVDTQTITIKQLGQDVVLKKGDKVFVSFVYASKDASVFPDPLDIKLDRPAELYVTHGEGQHQCLGKDINIIQNTYMLKTLAKLRNFRRAPGDEGKLKFISKPGGIKLYMNADWSKFTPYPTTMRVQFDGPIVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFN0</accession>
    <name>D5GFN0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Elongation factor 1-alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">ERF-3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">ERF2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Eukaryotic peptide chain release factor GTP-binding subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Polypeptide release factor 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Translation release factor 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">GSTUM_00006992001</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430209">
        <property type="protein sequence ID" value="CAZ83323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839132.1">
        <property type="nucleotide sequence ID" value="XM_002839086.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFN0"/>
    <dbReference type="FunCoup" id="D5GFN0">
        <property type="interactions" value="870"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GFN0"/>
    <dbReference type="GeneID" id="9188271"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006992001"/>
    <dbReference type="eggNOG" id="KOG0459">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007265_3_8_1"/>
    <dbReference type="InParanoid" id="D5GFN0"/>
    <dbReference type="OMA" id="TNESVCA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018444">
        <property type="term" value="C:translation release factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003747">
        <property type="term" value="F:translation release factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002184">
        <property type="term" value="P:cytoplasmic translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000288">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01883">
        <property type="entry name" value="EF1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03704">
        <property type="entry name" value="eRF3_C_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04089">
        <property type="entry name" value="eRF3_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000017">
        <property type="entry name" value="Eukaryotic peptide chain release factor GTP-binding subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000503">
        <property type="entry name" value="Peptide chain release factor subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000020">
        <property type="entry name" value="Translation elongation factor EF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054696">
        <property type="entry name" value="GTP-eEF1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003285">
        <property type="entry name" value="Sup35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050100">
        <property type="entry name" value="TRAFAC_GTPase_members"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23115">
        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22594">
        <property type="entry name" value="GTP-eEF1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01343">
        <property type="entry name" value="YEASTERF"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Tr-type G" evidence="16">
        <location>
            <begin position="300"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="104"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="69"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="115"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="147"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="224"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAZ83323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="730" mass="78724" checksum="1D2BBC2CDD6C76F9" modified="2010-06-15" version="1">MSGNTPNSWEDAAGDDNDLARQAQDQLNLNRPQQQQQGSGFRPSYNAPTFQPGAASFTPGAPAFTPGAGSYGQAGYGAGYGQQYPQYGQYGQYGMQGGYEQYGQQYGNYGGGYGQQAAYGQQPGQFSGYQQQQQQQQPYRPPHILRQQQQQAAPQAAKKQDSAPASGTSSPTPSKSGTSTPITTTSAAAAPKVLKIGGDAAPKVLKIGGDPAPSHKREPDNKPVSGSGVAANKAVAASLAGGSGKTSPSPGGPPVGKSSPAPTAAGKAAQLAAEKREADAVARAQEDEIDDSVIEELYGKEHVNLIFIGHVDAGKSSLGGAILYATGMVDERTMEKYKRDAKEQGRESWYLSWALDLTKEERAKGKTVEVGRAYFETEKRRYTVLDAPGHKNYVPNMIGGASQADVGILVISARKGEYETGFEKGGQTREHAVLARTQGVNKLIVAVNKMDDPTVCWSKERFDECTTKLTQFLKGTGYSTKTDLMFMPLSALTGANLKTRVAEDVCPWYSGPSLLEYLDNMKTLERKLKTPFMMPISGKYKDMGTVIEGKVESGFIKKNSSLVMMPGKTPLEIVAIYGETEEELPHAQCGDQVRLRVRGIEEEDVIPGFVLSSPKKPIHCVTAFEAQIHILELKSILTAGFNCVMHVHTTIQEVTFAALLHKLEKGTGRKSRKPPAFANKGQAIIARLESATGSLFPIERYEDYQQLGRFTLRDQGQTIAIGKVTKLIVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFP3</accession>
    <name>D5GFP3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00001896001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430214">
        <property type="protein sequence ID" value="CAZ83336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839145.1">
        <property type="nucleotide sequence ID" value="XM_002839099.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFP3"/>
    <dbReference type="STRING" id="656061.D5GFP3"/>
    <dbReference type="GeneID" id="9188285"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001896001"/>
    <dbReference type="HOGENOM" id="CLU_2190379_0_0_1"/>
    <dbReference type="InParanoid" id="D5GFP3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="20"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="109" mass="11714" checksum="473FA3E32AAE983E" modified="2010-06-15" version="1" fragment="single">MEFERSAREAAQFHQRHPSIARTSISTASPISPTTLLAPPAQSLPFPSSSSAISTNGYLSPPDSRRTSGNEDGRQSLPSLQEALFDRLDKPFTTPSPMQTPPLGQGSFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFQ4</accession>
    <name>D5GFQ4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="11">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00007007001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29091"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="7.1.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the complex I 30 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430220">
        <property type="protein sequence ID" value="CAZ83347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839156.1">
        <property type="nucleotide sequence ID" value="XM_002839110.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFQ4"/>
    <dbReference type="STRING" id="656061.D5GFQ4"/>
    <dbReference type="GeneID" id="9188295"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007007001"/>
    <dbReference type="eggNOG" id="KOG1713">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042628_0_0_1"/>
    <dbReference type="InParanoid" id="D5GFQ4"/>
    <dbReference type="OMA" id="PCRKNRF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.80:FF:000002">
        <property type="entry name" value="NADH dehydrogenase iron-sulfur protein 3, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.80">
        <property type="entry name" value="NADH:ubiquinone oxidoreductase, 30kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01357">
        <property type="entry name" value="NDH1_NuoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010218">
        <property type="entry name" value="NADH_DH_suC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037232">
        <property type="entry name" value="NADH_quin_OxRdtase_su_C/D-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001268">
        <property type="entry name" value="NADH_UbQ_OxRdtase_30kDa_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020396">
        <property type="entry name" value="NADH_UbQ_OxRdtase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01961">
        <property type="entry name" value="NuoC_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10884:SF14">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10884">
        <property type="entry name" value="NADH DEHYDROGENASE UBIQUINONE IRON-SULFUR PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00329">
        <property type="entry name" value="Complex1_30kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143243">
        <property type="entry name" value="Nqo5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00542">
        <property type="entry name" value="COMPLEX1_30K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0520">NAD</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="NADH:ubiquinone oxidoreductase 30kDa subunit" evidence="10">
        <location>
            <begin position="114"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="248"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ83347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="277" mass="31293" checksum="66AF36B662CE76AE" modified="2010-06-15" version="1">MSLRINSSTVIVRSLPAGARSPAIRLAPTITARAYHSGARRPDGTELPNIRKAERPPIGPLNAPIVNPADKYKSSSESLHAYGRYLISALPKYIQQFSIWKDELTIYIAPSAILPYTQVSDITAVDYPTRINRFEVVYNLLSIRHNSRIRVKTYADEASPVPSITGLYDGANWYEREVYDLFGVLFVGHPDLRRIMTDYGFDGHPLRKDFPLTGYTELRYDEEKKRIVVEPLEMTQAFRNFQGGSSVWEQVGSGKDDKPDSFKLPTPKSETKPAEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GG95</accession>
    <name>D5GG95_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007276001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430253">
        <property type="protein sequence ID" value="CAZ83538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839347.1">
        <property type="nucleotide sequence ID" value="XM_002839301.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GG95"/>
    <dbReference type="GeneID" id="9184600"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007276001"/>
    <dbReference type="HOGENOM" id="CLU_3224905_0_0_1"/>
    <dbReference type="InParanoid" id="D5GG95"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="44" mass="5254" checksum="A5FE82F0160517D2" modified="2010-06-15" version="1">MVMGPFFFSRSLGLAFNDMFHGWLLFRSSPLGIFFFFSVIEDWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGI3</accession>
    <name>D5GGI3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007400001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430264">
        <property type="protein sequence ID" value="CAZ83626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839435.1">
        <property type="nucleotide sequence ID" value="XM_002839389.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGI3"/>
    <dbReference type="GeneID" id="9185548"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007400001"/>
    <dbReference type="HOGENOM" id="CLU_462462_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGI3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="311"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="355"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="432"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="130"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="218"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="438"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="463"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="590" mass="62839" checksum="4F8771B1CB634AC6" modified="2010-06-15" version="1">MRGEHRESAPTIPQSGAEAQGGSSLRVVRKKGSLRSMFGSFLKSAKGRRANKTASQYPSRGGEAGAKNRGNEAPTLSAISLPTQARSSTSSTSSPYEDDGEFSNIVPYKEHGASCKTCSATSLPAQIRPPFAFHAPSSSSSSTSSIEFASDASTAATSPSVSSPSVLIKSPSSQSISALNHSTTSLSSTPSRETSPTRAQSSAKSSMMSKRALENHSKQPSPLSTVTDAHSPYILPTTPESPVLHERPNSFQPKRRGSLSNFILRRTQSHPDLTDFTTDDLPPFLQMPISAPTPRPGLSISSLSTRTLSLRRRSGKADIEAPTSYSTQEKIEHRSILGADPAGAGFYLYPTAQSEISGRRSSSGSSPGGSRSGSWGRAPMGGNMGDDSRRSSWTGPDGHNARGMPPAGRGWRDPRGGRSFGRSVTQQVILEEVGDVADSPQTTYSSSSVPSFDSQQAALRSGGLSNQNRSPDMTKNVSVPEKSERKRSPHNNWDSIQSQMQILKGDFPQLQVPPETRKGAEDCGMGEKMALRSLQQRAMRFMDASSADGNGSRGTPVTIEFEHPNRLSAVGNSRGLIDSLPIVRIESVEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGW4</accession>
    <name>D5GGW4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00002068001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430284">
        <property type="protein sequence ID" value="CAZ83757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839566.1">
        <property type="nucleotide sequence ID" value="XM_002839520.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGW4"/>
    <dbReference type="GeneID" id="9181728"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002068001"/>
    <dbReference type="HOGENOM" id="CLU_3160284_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGW4"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ83757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="48" mass="5299" checksum="C8D7D0DD65DD42A1" modified="2010-06-15" version="1">MMFNFSSFSSSICFSAIAIRSCIASAYKNFLLLLLLLFFFSSSSTKGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>D5GH76</accession>
    <name>D5GH76_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00007782001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430308">
        <property type="protein sequence ID" value="CAZ83901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839710.1">
        <property type="nucleotide sequence ID" value="XM_002839664.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GH76"/>
    <dbReference type="GeneID" id="9182606"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007782001"/>
    <dbReference type="HOGENOM" id="CLU_1332778_0_0_1"/>
    <dbReference type="InParanoid" id="D5GH76"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052374">
        <property type="entry name" value="SERAC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48182">
        <property type="entry name" value="PROTEIN SERAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48182:SF2">
        <property type="entry name" value="PROTEIN SERAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ83901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="206" mass="22842" checksum="4469D31E439B0DFC" modified="2010-06-15" version="1">MTYNYNLVTGPQDFISIGSIKNAALELLRELSRGRRILGQAGKRDKIAEPRPLKRAPTLPIDQTRPIIFIGYQLGGTIIKQALVFANYEKLYMPISSEISLLVFFGTPHRAATPAQWESAAEMLLSSSGSKEISSSVLKSSSKALMDSSAGFMPFLGAIPVLNFYDNRLHMVSILYLYSLSMDTLYFPINIYYVENVVSISAGFLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHA3</accession>
    <name>D5GHA3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00007718001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430308">
        <property type="protein sequence ID" value="CAZ83855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839664.1">
        <property type="nucleotide sequence ID" value="XM_002839618.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHA3"/>
    <dbReference type="FunCoup" id="D5GHA3">
        <property type="interactions" value="418"/>
    </dbReference>
    <dbReference type="GeneID" id="9182560"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007718001"/>
    <dbReference type="eggNOG" id="KOG2765">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026578_0_1_1"/>
    <dbReference type="InParanoid" id="D5GHA3"/>
    <dbReference type="OMA" id="MYGVYTI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025016">
        <property type="entry name" value="DUF3955"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23051:SF0">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23051">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35, MEMBER F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13127">
        <property type="entry name" value="DUF3955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="54"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="163"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="187"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="218"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="257"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="290"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="328"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="356"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="383"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3955" evidence="7">
        <location>
            <begin position="52"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="6">
        <location>
            <begin position="162"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="6">
        <location>
            <begin position="259"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ83855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="430" mass="47538" checksum="01181CE5CD2241B1" modified="2010-06-15" version="1">MSTSPLPPIHASFSPPPDFIVPLLPPPPARPSSLPLMPKPQPIRIRVFPRRKTIGILLLGVVIFLWVLSTFLTFTIFSDGSYTKPYFVTYVNTCSFCFYLLPGAWRAWRGKCEERDRSRVGYARLGNVPLPRAVGEDEDRDGVIMASKSLVVEGEGMLSERETILLSLQFCLLWFVANYFQSYCLKWTSVASATILSSTSSIFTLMLGALLRIERFTWTKFLAVVLSFTGVSLVSSVDLSPSSSLSPGAGDKTPGQILRGDLMALMGAFSYGVYTILLKVRIGHESRISMTRFFGFVGLFNFLVLWPGIIILHHAGVEKFEIPPDARIWWIVVINASITLISDYCWVYAMLLTTPLIVTVGLSLTIPLALLGQMLVLGVWSSGVYWIGAVLVFLAFLFVNRESVVDDIGGGEGEGGVVVDVEGETGRWER</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHC3</accession>
    <name>D5GHC3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007745001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430308">
        <property type="protein sequence ID" value="CAZ83875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839684.1">
        <property type="nucleotide sequence ID" value="XM_002839638.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHC3"/>
    <dbReference type="GeneID" id="9182580"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007745001"/>
    <dbReference type="HOGENOM" id="CLU_2559960_0_0_1"/>
    <dbReference type="InParanoid" id="D5GHC3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="82" mass="9255" checksum="43A58A8D6EA518D0" modified="2010-06-15" version="1">MENCIYDGGILQPGSPIAGKCWYSYSTPPTAQRDCKIVIENFNISYGRILLYCINVSIILISYFSASYRVYSYSTLLTPHLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHN3</accession>
    <name>D5GHN3_TUBMM</name>
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        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008057001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430320">
        <property type="protein sequence ID" value="CAZ84063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839872.1">
        <property type="nucleotide sequence ID" value="XM_002839826.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHN3"/>
    <dbReference type="GeneID" id="9183717"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008057001"/>
    <dbReference type="HOGENOM" id="CLU_2833020_0_0_1"/>
    <dbReference type="InParanoid" id="D5GHN3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="66" mass="7488" checksum="D0D61FEFF4358B06" modified="2010-06-15" version="1">MPRASSNSAPRKLETTGARRRHKLPTVRRNPRTKPPHVGNYQINTHHKTSLVHTTQTWGGRARGLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5GI69</accession>
    <name>D5GI69_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00008310001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YSP2 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430323">
        <property type="protein sequence ID" value="CAZ84212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840021.1">
        <property type="nucleotide sequence ID" value="XM_002839975.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GI69"/>
    <dbReference type="STRING" id="656061.D5GI69"/>
    <dbReference type="GeneID" id="9181930"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008310001"/>
    <dbReference type="eggNOG" id="KOG1032">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006864_1_0_1"/>
    <dbReference type="InParanoid" id="D5GI69"/>
    <dbReference type="OMA" id="PSGNMIS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140268">
        <property type="term" value="C:endoplasmic reticulum-plasma membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120015">
        <property type="term" value="F:sterol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032366">
        <property type="term" value="P:intracellular sterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13220">
        <property type="entry name" value="PH-GRAM_GRAMDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051482">
        <property type="entry name" value="Cholesterol_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004182">
        <property type="entry name" value="GRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031968">
        <property type="entry name" value="VASt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23319">
        <property type="entry name" value="GRAM DOMAIN CONTAINING 1B, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23319:SF4">
        <property type="entry name" value="GRAM DOMAIN CONTAINING 1B, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02893">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16016">
        <property type="entry name" value="VASt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00568">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51778">
        <property type="entry name" value="VAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="866"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="domain" description="VASt" evidence="8">
        <location>
            <begin position="644"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="174"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="230"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="314"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="541"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="44"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="135"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="237"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="253"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="546"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ84212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1004" mass="106366" checksum="A9936D2D4163099F" modified="2010-06-15" version="1">MSEDRNRRSTRSSVGTSDGSDIAAAIPPKVASSERDTKPWPSVLTETRAQPIVTSESGVNADGTIKTTVTPPTPTDSQQHFPPKSPVGAVQAGDNGMKKRGRTNSLSNAPSKLSQSMTVPLTPTIEGGATLDASQQPGQGNNTNGGFFQSMFSVAQNAATTLGNTIANANAANTAGGVRSRSGTGASESGKSETEQEIVRQGGGSGVEGEKNEPQKRPAIETLGQGELSLGSLGILPNSQKSSASPSEGRPSRSASVNSHGASSETGDSRVITGGGGTVLQIGASAGPAHRIGFTDGGLNDEVLLSLDSAVSSRGGFETAGESAFNSPVGPGGSYGLEGLRKTPMAEDSAFTGRDGDHAPEKGVLNPPDDRDDTWSGDADRRRSGSVRSGGRRKRGSSAASGANPQPVPRPTGFAVAPKRRNREFHEMFRSVPEDDYLIEDYGCALQKEILLQGRLYVSEGHICFYSNIFGWVNTLIISFDEIVSVEKKNTAMLFPNAIVIQTLHARNVFASFISRDSTYDLIVGIWKIGHPQLVAGATGVRLEDGGDAEEGDPPDGVSEYEEDGDDDEYEDASNDDLGESYTDAGDVVLSDDKGKNGGGASKSVSRKASQAVLGASGEDGSGAVVDYPGPQTHPPTSCNDDHYDRPLCDDIIPAPLGKVYSLVFGPASFPFMSQFLADEEKTRSFSYIKLLGGGIGPKQTKCNITETLDNCDLEDHVIVTATTQTPDVPSGNVFMVKTRYCLTWADDGQTRLVSSCTVEWSGKSWLKGSPIEKGANDGQIAYNKDLRIALKKEVEPKEPIGKQSKNKIKKRKKDLNAKGEAIGRAAGGTVKGREALAAGFESPAGQASWGVFAPLRTYLEPVADIISPLIGGAPGLVAACLVLAVFLWWQSTSVYSRHANTAVDGGSGVHHLHQHWDTLWRSEEDGLWEWLEDRVGINEVVAPPSRRANSFEKAARSRRDGAGAGLTQGMKRREVEDAIGVMEERLAILRRVVEEGRYGDGTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>D5GI88</accession>
    <name>D5GI88_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008338001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430324">
        <property type="protein sequence ID" value="CAZ84231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840040.1">
        <property type="nucleotide sequence ID" value="XM_002839994.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GI88"/>
    <dbReference type="GeneID" id="9181949"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008338001"/>
    <dbReference type="HOGENOM" id="CLU_429716_0_0_1"/>
    <dbReference type="InParanoid" id="D5GI88"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="505"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="585"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="508"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="528"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="610"/>
            <end position="622"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="637" mass="72510" checksum="1023DFF9BC3689DF" modified="2010-06-15" version="1">MEPHRQYSPIETALLCHEPIVSSVLAYLAVHEVVILASTCHPLRSFLFSHSGYWRETNLCPCPPELALNPRVMRWDGPTEQLDHWRHKLLSVYKAPYNRPDDYELLTIFLRTLFVRNLVPTIQITKLVFDGLPIKRDIFFEITDRLSPCLRELSVRACAELEMSDFIKLLDFQEPARQSHFPPNLKKLSCFGTVVYDEPYEIRTMSLSHHTRKSLQLSLSHFRTLLIARSLECDIGPCLQCEERKLGLRGGDVCANCGKADNIVRCISCEHMDNLGKRCASCRRGFCSPCRDKVMERLPDQPSFCKECIEHVSSFPGCVKCNRSMPPSTDYPLGKDYGITGSTQDVCGLCHKWMCMACIAGEYPNEEGDSKRWTIRQCTNCFNNICKECGEKYSEEDYEKVLNLPAVREAISLCTIYRDVTEALGATPPPDATISVPGGKNCTVSSESVRVMARDRFKQVGPPAIYNLVIQRAETWKPLLPPCEECEQWWCWECRLRCTCPEAETDTELPNGNAESSNSADKTPETVDWSEMDSESVDGMDQDSNESIDQRQYPCLVHNKALDNIHPAIEEFCWSCGLWPREYTDEQDERPEHTTSAAPSPATDGRTNTGEDEDMDSSDEWEWYSHRSHASVASWDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>D5GI93</accession>
    <name>D5GI93_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008348001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430324">
        <property type="protein sequence ID" value="CAZ84236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840045.1">
        <property type="nucleotide sequence ID" value="XM_002839999.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GI93"/>
    <dbReference type="STRING" id="656061.D5GI93"/>
    <dbReference type="GeneID" id="9181954"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008348001"/>
    <dbReference type="HOGENOM" id="CLU_195481_0_0_1"/>
    <dbReference type="InParanoid" id="D5GI93"/>
    <dbReference type="OMA" id="CTYWFAR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37919:SF2">
        <property type="entry name" value="EXPERA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37919">
        <property type="entry name" value="PROTEIN CBG05606"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="81" mass="8964" checksum="8C6C91ED0093921D" modified="2010-06-15" version="1">MLVAGFAGVLMTLAKTTLFVLNEFCAGGRHVAHNDLKNFVLFYVLPNGLWIAFPGWCTYWFAREIVKGIDTGSGGKVKKRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIC1</accession>
    <name>D5GIC1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00008419001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430326">
        <property type="protein sequence ID" value="CAZ84264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840073.1">
        <property type="nucleotide sequence ID" value="XM_002840027.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIC1"/>
    <dbReference type="STRING" id="656061.D5GIC1"/>
    <dbReference type="GeneID" id="9184622"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008419001"/>
    <dbReference type="eggNOG" id="ENOG502S1KY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028410_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIC1"/>
    <dbReference type="OMA" id="CKEICEN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04370">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001025">
        <property type="entry name" value="BAH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043151">
        <property type="entry name" value="BAH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46364">
        <property type="entry name" value="OS08G0421900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01426">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00439">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51038">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BAH" evidence="2">
        <location>
            <begin position="129"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="294"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="61"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="305"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="336"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ84264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="411" mass="46170" checksum="BA41E6C02DCE8126" modified="2010-06-15" version="1">MPPKRKRGGGVSENAVESPEEQQSEPSVNNNPPLIVSDCPFTVEYKAATPAAKKKKKKKGGAASAGGSHAPRQAKRYTKSGVPINPVGASRKHQEIDREVPFDSTLYAIKPRKQWDQLTKYRSFVVGTENFALNEYIFVNHSNIPHGTDLGASDDKKFWVARVLEIRAIDESHVYLRVYWLYWPEELPGGRQPYHGAKEIIASNHMEIIDAMTVSGRATVKHWMELDEEEDLPDLFWRQKFDYPTQMLMEVREHCKCRGYYNPDKIMYACTSCKIWLHEECLIADIKKKLYKGITEGPPSPTNDSQEEEDSEEEDESITAANRNRGNKRTSAAVATASNSKPQTPKSSNARGKNAVAGSEKEDFFQVIIRPEVNGPVIAAVRDLRNRKEWSERGGKWEEGVGCLGCGSVIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIC3</accession>
    <name>D5GIC3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00008421001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430326">
        <property type="protein sequence ID" value="CAZ84266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840075.1">
        <property type="nucleotide sequence ID" value="XM_002840029.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIC3"/>
    <dbReference type="STRING" id="656061.D5GIC3"/>
    <dbReference type="GeneID" id="9184624"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008421001"/>
    <dbReference type="eggNOG" id="KOG3958">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038287_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIC3"/>
    <dbReference type="OMA" id="SDMDIQP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005869">
        <property type="term" value="C:dynactin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028133">
        <property type="entry name" value="Dynamitin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15346">
        <property type="entry name" value="DYNACTIN SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04912">
        <property type="entry name" value="Dynamitin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="85"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="306"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="121"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="30"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="87"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="106"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="306"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ84266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="442" mass="48340" checksum="BB7F07BD41AA089E" modified="2010-06-15" version="1">MSSKYAALPDLDISQDVYETPDLAEDISTFQTSTSAAHSASGSEVGEDANGCGTISYDHLRLAAARGSFKHTEIDATGVDFSDRISGKRRSYRASSRRRRRRAGAYDEEYDDDDDDDGLGEETLEKRVARLKKEVEEVRGEIEAREQELGDGMEEDGTVQGGARKGEEGVCVDDEDEDILDVVEFKSSIDELSNKASENAPITTDGSTATYTVTYAPAFKQSHALAKAADFDKRLTVLEKVLGMSASATVNVDKELPNSAMIPTLDELSRQMSVLTAPSTTSLDAANKRVKQLTLEAERLAEARKAAKATADARREEADQLSEGSTAPLADMSEREAKINALYGALPTIENLGPLLPAVLDRLRSLRTIHADAGKAVENLTKLERRQEEMGGEIARWRAALEKVETVVKESEGTIQGNMGKVEGWVQELEEKVRMVEKMVER</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIF4</accession>
    <name>D5GIF4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008473001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430326">
        <property type="protein sequence ID" value="CAZ84297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840106.1">
        <property type="nucleotide sequence ID" value="XM_002840060.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIF4"/>
    <dbReference type="GeneID" id="9184655"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008473001"/>
    <dbReference type="HOGENOM" id="CLU_2198914_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIF4"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="108" mass="11784" checksum="81F3C5B5D35D0A4E" modified="2010-06-15" version="1">MNPLYPALDYLPPRPPPRHNRTGVTINTYVTGRPAGGAGKDEATRGETKPGQAVSRQDYGRWAGQPEAKRHRGAVGARSRPQLKTKCGRHNTGSAAGRRHCNPLDEYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJ19</accession>
    <name>D5GJ19_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008783001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840321.1">
        <property type="nucleotide sequence ID" value="XM_002840275.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJ19"/>
    <dbReference type="GeneID" id="9181853"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008783001"/>
    <dbReference type="HOGENOM" id="CLU_2575588_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJ19"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="81" mass="9372" checksum="A21D9ADA6B37DC05" modified="2010-06-15" version="1">MVANLPHRPICSFSRPSLTIALLELTTTTSTANPYHHSISYIPYHIPHSQAKFSMKTKSKTQDTKQNNRRKNLMRIFEEKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJG8</accession>
    <name>D5GJG8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="11">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00008992001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the multicopper oxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430330">
        <property type="protein sequence ID" value="CAZ84661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840470.1">
        <property type="nucleotide sequence ID" value="XM_002840424.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJG8"/>
    <dbReference type="STRING" id="656061.D5GJG8"/>
    <dbReference type="GeneID" id="9182662"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008992001"/>
    <dbReference type="eggNOG" id="KOG1263">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006504_3_2_1"/>
    <dbReference type="InParanoid" id="D5GJG8"/>
    <dbReference type="OMA" id="TGAWAFH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13854">
        <property type="entry name" value="CuRO_1_MaLCC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13880">
        <property type="entry name" value="CuRO_2_MaLCC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13901">
        <property type="entry name" value="CuRO_3_MaLCC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000021">
        <property type="entry name" value="Extracellular dihydrogeodin oxidase/laccase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000038">
        <property type="entry name" value="Extracellular dihydrogeodin oxidase/laccase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF502">
        <property type="entry name" value="MULTICOPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0186">Copper</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072104" evidence="7">
        <location>
            <begin position="19"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="10">
        <location>
            <begin position="80"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="8">
        <location>
            <begin position="205"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="9">
        <location>
            <begin position="429"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ84661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="586" mass="65184" checksum="6F30976D0ED08CCF" modified="2010-06-15" version="1">MHLGDFLGIVIITTTASALPSLFTAPTTEVLAPKLFSKRIANCAHGPTTRNCWDGGRDINTDYYSNFPLTGRVREYWLRVENSTFAPDGYQRTVLTFNGTLPGPTLHADWGDTMRIHVTNGMEYNGTSIHWHGVRQLDTTEQDGVNGITECPFPPGYSLTYEWKATQYGTSWYHSHFSLQYADGVAGALVINGPASANYDEDLGPIMLDDWYHSTAFGLWQHIPTTGPQAANNGLINGMNVFDCSGSTDPNCVGGGKRFLTKFKPGKKYLLRFVNIATDTFFKVTLDNHTMTVISMDFVPITPYETEFVSIGIGERYQVIIEANQATDNYWLRAISQTSCGAANPNRLNIKGIVRYSGAANREPTTSARAIPDSCADEPKDKLVPVVAKSVPPSDLSISQRENVSMTISDNRIHWKLNGISAKIDWLNPTLLHVLKGQTFPEDYNVLEIDGSNPWYYLVIETDLPIEHPIHLHGHDFYLIAQESGTFDTSKVKPKWSNPPRRDVAMLPGSGYMVIAFQTDNPGVWLLHCHIAWHVSGGFALQFVERKTDLLKRFNPAGAWTDTCSAWNTYISSDIDPSQEDSGVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJL4</accession>
    <name>D5GJL4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">GPI ethanolamine phosphate transferase 1</fullName>
            <ecNumber evidence="14">2.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">GSTUM_00009065001</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13 14">Ethanolamine phosphate transferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers ethanolamine phosphate to the first alpha-1,4-linked mannose of the glycosylphosphatidylinositol precursor of GPI-anchor.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Endoplasmic reticulum membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the PIGG/PIGN/PIGO family. PIGN subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.-.-.-" evidence="14"/>
    <dbReference type="EMBL" id="FN430332">
        <property type="protein sequence ID" value="CAZ84707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840516.1">
        <property type="nucleotide sequence ID" value="XM_002840470.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJL4"/>
    <dbReference type="FunCoup" id="D5GJL4">
        <property type="interactions" value="465"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJL4"/>
    <dbReference type="GeneID" id="9186741"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009065001"/>
    <dbReference type="eggNOG" id="KOG2124">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007676_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJL4"/>
    <dbReference type="OMA" id="QSYFHRE"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051377">
        <property type="term" value="F:mannose-ethanolamine phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16020">
        <property type="entry name" value="GPI_EPT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000015">
        <property type="entry name" value="GPI ethanolamine phosphate transferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007070">
        <property type="entry name" value="GPI_EtnP_transferase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017852">
        <property type="entry name" value="GPI_EtnP_transferase_1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037671">
        <property type="entry name" value="PIGN_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12250:SF0">
        <property type="entry name" value="GPI ETHANOLAMINE PHOSPHATE TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12250">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL GLYCAN, CLASS N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04987">
        <property type="entry name" value="PigN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 14" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="455"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="491"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="555"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="582"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="607"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="634"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="711"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="736"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="759"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="796"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="837"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="872"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="903"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="domain" description="GPI ethanolamine phosphate transferase 1 C-terminal" evidence="16">
        <location>
            <begin position="444"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="937"/>
            <end position="972"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU367138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAZ84707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="972" mass="107590" checksum="4A3FA66221A33952" modified="2010-06-15" version="1">MARLGRLGFLGLAVLFHLIYIYSIFDIYFVSPIVHGMQQYHVDAPAPAKRLFLIVGDGLRADKCFQSHDDPDYPSENRYLAPFIRSKVLNDATFGVSHTRVPTESRPGHVALIAGLYEDVSAVTTGWKLNPVNFDSVFNESRHTWSWGSPDILPMFKHGASDESRVDAFSYDEEMEDFTADSTNLDIWVFDRVKELFESAARNNTINTMLRQDKVVFFLHLLGLDTAGHSYRPYSAEYLRNIKVVDEGVQQIHKLVNDFYGNDDKTSWIFTADHGMSDWGSHGDGHPDNTRTPLVAWGAGIKKPVASKVLEVTGHEDGFSGDWGLGAVQRVDVAQADVAALMAYLIGLNFPVNSVGELPLEYLDANASMKAEASLANARQILEMYKVKEEQKKSTTLRYTAFVPLSEGKQSLESRFAEIRGLIDSGSHERAIEKCVELIKVGLEGLRYLQTYDWLFLRTLVTAGYLGWIAFSLTTVIDLHVLHGRTEPTRNIGSSIFFISSLVAIYSVLMIQKSSWTYYAYAFFPVMFWEEVYARKNSVTEGTRKLVAHLDSRSAYMGLGAEIIGFLVLLEAIVYGYFHREVFTGCFLLATAWPLLYGSDFLRKNKLLAAGWIACCCSMSTFTLLPVIKQESESLILLGGFFMFSLGLVYMTFGETAANGSDGAQVARGQDWVSKSILGSQLGLLGLSMFVTRSSVRSLQAKLGLPLGNQAVGWLVLVSSIACPFLHGIRPQSHYLHRLMAIFLTFSPIFVILSISYEGLFYVIFCLTLVAWVQLEHKIHQHSHRKMQEGLTLADARIALFFFFLIQVAFFGTGNVASLSSFSLESVYRLIPVFNPLAMGALLVLKLMVPFAVISANLGILNRRIGVAPSSLFMVVMAISDVLTLNFFYMVRDEGSWLDIGTTISHFCISSMLGVFVSALEFMSEVFVGGVSMEGGNAMPSSPSSPASKGGARVENGESTRGSNGEVANGAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJP1</accession>
    <name>D5GJP1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00009093001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430332">
        <property type="protein sequence ID" value="CAZ84734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840543.1">
        <property type="nucleotide sequence ID" value="XM_002840497.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJP1"/>
    <dbReference type="GeneID" id="9186768"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009093001"/>
    <dbReference type="HOGENOM" id="CLU_2456400_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJP1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ84734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="89" mass="9720" checksum="52550772C92BCC5A" modified="2010-06-15" version="1">MEAFSIPGARDGRLCNCTVPCSWYDRAVRGSSKRGGGKVGDAAIDVFCKRLTVATGQIAKERTHTHTKKKTPVGYSRARVSERYSITIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJP6</accession>
    <name>D5GJP6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00009114001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430332">
        <property type="protein sequence ID" value="CAZ84739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840548.1">
        <property type="nucleotide sequence ID" value="XM_002840502.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJP6"/>
    <dbReference type="FunCoup" id="D5GJP6">
        <property type="interactions" value="1362"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJP6"/>
    <dbReference type="GeneID" id="9186773"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009114001"/>
    <dbReference type="eggNOG" id="KOG0270">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023867_0_1_1"/>
    <dbReference type="InParanoid" id="D5GJP6"/>
    <dbReference type="OMA" id="CFVPRGV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000457">
        <property type="entry name" value="rRNA processing protein Pwp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044285">
        <property type="entry name" value="PWP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14091">
        <property type="entry name" value="PERIODIC TRYPTOPHAN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14091:SF0">
        <property type="entry name" value="PERIODIC TRYPTOPHAN PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="273"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="414"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="42"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="247"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="522"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="54"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="247"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="257"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="531"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="552"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ84739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="563" mass="61620" checksum="18E9D7CDBEF4ED92" modified="2010-06-15" version="1">MITATTWVPRGFAAQFPQRYDIDEKEFERIADLARLHLEDAKEGLEEAEAAGSDGRKEEEEGEKNGESNGAAAEAVDKDEDMKEVGDEKQREFVSLSLYTSNCPLKSGFSGMGMFGNIKGLAYYQPGEQDPYITMDLQDDDEEREELQVLGTDNLLLVAKTEDDIAHLEVYVYEDEADNLYVHHDIMLPAIPLCLEWLDIPVGGASVLENKDARGNYAAIGTMDPDIEIWNLDLVDCLFPNAILGASSEPSPASVSKSKKKKGKKKKSPKVNDNYHIDAVLSLSGNRQHRNLLASASADKTVKLWDLSTTKCAKSYNSLHTDKICALSWHPSSATTILSGSYDRTIVWSDMRSPDPTSTNPRWGVDADVEDVRWDPHNDHYFYVSTESGNLYLYDSRNSPPASTLAQSKPVWTLNAHDKSLSAFDVNPVIPGFIVTASVDRSVKLWNARAENTGGGPAMVVSRDFDLGKIFSARFAPDKEVGFRLAVAGSKGIVKVWDTSTNAGVRRAFAGRVAGVVGEEREERVVGAVEDSSDGSDEEEDGGVTVGGGDGEGPDGWESMEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJU4</accession>
    <name>D5GJU4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00009197001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430333">
        <property type="protein sequence ID" value="CAZ84787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840596.1">
        <property type="nucleotide sequence ID" value="XM_002840550.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJU4"/>
    <dbReference type="GeneID" id="9186823"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009197001"/>
    <dbReference type="HOGENOM" id="CLU_2887467_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJU4"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ84787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="63" mass="7094" checksum="5FE53A7B3A20B0C9" modified="2010-06-15" version="1">MAKFRASFFLSGTQLKISPKVPPDDCVHQRIERRIQGYSPTLCLQRSLPSWDESIVAMGLMGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKA7</accession>
    <name>D5GKA7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00009451001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430337">
        <property type="protein sequence ID" value="CAZ84950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840759.1">
        <property type="nucleotide sequence ID" value="XM_002840713.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKA7"/>
    <dbReference type="STRING" id="656061.D5GKA7"/>
    <dbReference type="GeneID" id="9181970"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009451001"/>
    <dbReference type="eggNOG" id="KOG3091">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023804_0_1_1"/>
    <dbReference type="InParanoid" id="D5GKA7"/>
    <dbReference type="OMA" id="MMQTRLH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044613">
        <property type="term" value="C:nuclear pore central transport channel"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006607">
        <property type="term" value="P:NLS-bearing protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006999">
        <property type="term" value="P:nuclear pore organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036228">
        <property type="term" value="P:protein localization to nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.490">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025574">
        <property type="entry name" value="Nucleoporin_FG_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024864">
        <property type="entry name" value="Nup54/Nup57/Nup44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025712">
        <property type="entry name" value="Nup54_alpha-helical_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13000">
        <property type="entry name" value="NUCLEOPORIN P54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13000:SF0">
        <property type="entry name" value="NUCLEOPORIN P54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13634">
        <property type="entry name" value="Nucleoporin_FG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13874">
        <property type="entry name" value="Nup54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="3" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0811">Translocation</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nucleoporin Nup54 alpha-helical" evidence="7">
        <location>
            <begin position="277"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="313"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="9"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="56"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="87"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="132"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="150"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="162"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="180"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="191"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ84950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="482" mass="52527" checksum="7EC4DAB3DEAF2079" modified="2010-06-15" version="1">MSLFGSTFGQPQQQQQQSQGGGLPEQPAAQQPQQSGGLFGGAPQQQQQSTGGGLFGSQQPQQQQQQGGLFGKPSGLFGQQSTPTTQQGGGLFGGGQSTQQSTGGGLFGGGGGGGGQQSAGGGMFGSQQQQGGLFGGGQSSQPTGGGLFGGQSTHQPQQQFGQQGGSLFGQSRTGGGLFGQTPSQPQQQQQGELFSNSTANQGQQQGSYMNSTMGGYGQQSMFPMMSQSMRVQQQSANVIVDKLGRIKNAWDTGHPDYAFKFYLYNHVGEDRANMYQKPPADDLAEWEKAWVERPNKGSVPVLAKGFKDLDYRVKSQEQQVNLFRHRLHEIQDKLAALQSRHDLMTSIRLEDCRRRHTALSRRALSLAAKVQVMKNRGYALQPEEEQLKKRLEALSRAVWDPAVRGRVNEIWARMMVVSEQARMMEATVGKVEIVWDEKQLQTAGKLLQTNAAGLEHLNKEVRDIEKAFDQWEEEQKSRPRGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKB5</accession>
    <name>D5GKB5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00009461001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ATPase epsilon family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430337">
        <property type="protein sequence ID" value="CAZ84958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840767.1">
        <property type="nucleotide sequence ID" value="XM_002840721.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKB5"/>
    <dbReference type="STRING" id="656061.D5GKB5"/>
    <dbReference type="GeneID" id="9181978"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009461001"/>
    <dbReference type="eggNOG" id="KOG3495">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_187039_0_0_1"/>
    <dbReference type="InParanoid" id="D5GKB5"/>
    <dbReference type="OMA" id="NKYTQIA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042776">
        <property type="term" value="P:proton motive force-driven mitochondrial ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12153">
        <property type="entry name" value="F1-ATPase_epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1620.20:FF:000003">
        <property type="entry name" value="Mitochondrial ATP synthase epsilon chain domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1620.20">
        <property type="entry name" value="ATP synthase, F1 complex, epsilon subunit superfamily, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006721">
        <property type="entry name" value="ATP_synth_F1_esu_mt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036742">
        <property type="entry name" value="ATP_synth_F1_esu_sf_mt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12448">
        <property type="entry name" value="ATP SYNTHASE EPSILON CHAIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12448:SF0">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT EPSILON, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04627">
        <property type="entry name" value="ATP-synt_Eps"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48690">
        <property type="entry name" value="Epsilon subunit of mitochondrial F1F0-ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="65" mass="7307" checksum="0E52BB4FDDAF086F" modified="2010-06-15" version="1">MVAAWRAAGLTYNKYLSIAARVVRRSLKEQPRLAAERRGEMQLKFAKWEGGKQGDVKSLAKESAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKF2</accession>
    <name>D5GKF2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00009510001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430338">
        <property type="protein sequence ID" value="CAZ84995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840804.1">
        <property type="nucleotide sequence ID" value="XM_002840758.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKF2"/>
    <dbReference type="STRING" id="656061.D5GKF2"/>
    <dbReference type="GeneID" id="9182015"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009510001"/>
    <dbReference type="HOGENOM" id="CLU_418084_0_0_1"/>
    <dbReference type="InParanoid" id="D5GKF2"/>
    <dbReference type="OMA" id="NGFFETF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="13"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="632"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="101"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="123"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="239"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="297"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="346"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="396"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="406"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="473"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="506"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="517"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="536"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="656" mass="70392" checksum="A340D6D23A4574AE" modified="2010-06-15" version="1">MANNYQPYVEDYNSELSEAIPVVPVRRQTTKRPSGLNQSSTVEEVPEPSYEGVSDSGYSSHSVASTPITETPPPPPKAPTPAPVRTHQPSRSSSGPIPFNRPSSKSFSRPASSAARNIPNANSGSYGYSSSYGAGRESSLGGGSSDCDCPDCGKTLSKSSPTISSSSSATSSAWPVHQQSPTYHHSLPYGQSPSYSGYHYPQHYDGYSTPTDSGISDSSAGAGGSRKSRSSMPPPPRPQSTFAGSTTTTSTHSSGSIPWSHTSSYSSASYFDAHPSTSPVPPAPSTTCPPVYGSSMYPPPPLDTSIAPPPPQSSSYSNSPYAPPPVPNYGYPPVTDYTANDYYGQSTAPSTPSSSLPPSDYRDGYRSSEYSVMPPPPPIANRRSSMRAQANAGASIDSSPDYTHSTPHRPQRRMSSRAHGSERSSSWYGQSQLQYQEAPERSYSVPPTNTGAPAESSRRRGTTPQPMRRRESNNSQGTSGSHSARLGPSALSRSMEACTIDDHTPRSSHHRHSSSGHHYDSHHAVARREPGTMMQSSRSNGSSDSGSSSGHEGREELVQLTVPDSDESFTMRYPAGIPVKLQLNGGTFQRILSFGRKSKNVNIEDPIAYGCVRQRQQQVHAQLQYAAGQLDHSDRRTSAEYHHRSNGYPRREMAGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKT5</accession>
    <name>D5GKT5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein artemis</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">DNA cross-link repair 1C protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">GSTUM_00009761001</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA repair metallo-beta-lactamase (DRMBL) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430341">
        <property type="protein sequence ID" value="CAZ85128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840937.1">
        <property type="nucleotide sequence ID" value="XM_002840891.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKT5"/>
    <dbReference type="STRING" id="656061.D5GKT5"/>
    <dbReference type="GeneID" id="9183364"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009761001"/>
    <dbReference type="eggNOG" id="KOG1361">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013294_0_1_1"/>
    <dbReference type="InParanoid" id="D5GKT5"/>
    <dbReference type="OMA" id="VFYFRAW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035312">
        <property type="term" value="F:5'-3' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12650">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011084">
        <property type="entry name" value="DRMBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23240">
        <property type="entry name" value="DNA CROSS-LINK REPAIR PROTEIN PSO2/SNM1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23240:SF8">
        <property type="entry name" value="PROTEIN ARTEMIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23023">
        <property type="entry name" value="Anti-Pycsar_Apyc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07522">
        <property type="entry name" value="DRMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="15">
        <location>
            <begin position="32"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="583"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="546"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="590"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="614"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="646"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="680"/>
            <end position="690"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00042677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAZ85128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="747" mass="84045" checksum="C35FE26EBCCF8949" modified="2010-06-15" version="1">MPDAAYPVLLLAAALNLKLYFDLILIPGSKSLSSVALIFDEFGGKFIMSTFKGIVTEFPDIRIDYFRQSNDSGARPALACFLTHIHSDHLQGLEGFYGGPFIYCSAATKELLIKLERRLHRFNLAKGVLEARRCLYAEKEKKLKVIPLETPTMVEIGSGKSLRVTLFDANHCPGAVMFLIEGDGKAILYTGDIRAEKWWVDYLKRHPVLVPYAMGLKTLDKIYLDTSFAGRSKIHEVFRSKQDGIHELLSQVRKYPQDTIFHLNAWTFGYEEAWVALASAFDSKIHLSDYYRRLFHFIRSPDKYVNGPFLSGFEFGNSKVLGCTTSNTEARFHSCEKRIGCKGLEGRKNIVWISPVITRVGSYDVHEPGVGSGDLENQDDLELRDEHLKQILEILGPDVPESVRDLLKEARTSMKQLLPLSLQRGSEEDDLTAPKLLGLLSEAAGISSQRKSQETDSQILIPTPISNDEEGVVAENGEILPNVITFPFSRHSSYSELRGLVSAFRPRDIYPCVVNEAAWDEEKSVETLFGDLCTGGEFTHDNDMRRRLEEEHMAQEQLEEERLERELLESQRNLDRGQLERIEINFPQCRPEERATEGSKDTEDQRWIQARAMASSTASNTPTTSPAASPKRPERARNVTPVAPVVSPPLDSPPPKQVKREPHTSPSSSPPTGSAKRIKIQNSLHGTVSSPRRPGKGVRMSSVEREFGDSEKFWEGVEAALGIGGTWWDVELSCTMQEWRYEEEVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKY4</accession>
    <name>D5GKY4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="12">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00009831001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430342">
        <property type="protein sequence ID" value="CAZ85177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840986.1">
        <property type="nucleotide sequence ID" value="XM_002840940.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKY4"/>
    <dbReference type="FunCoup" id="D5GKY4">
        <property type="interactions" value="57"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GKY4"/>
    <dbReference type="GeneID" id="9185771"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009831001"/>
    <dbReference type="eggNOG" id="KOG0769">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015166_6_3_1"/>
    <dbReference type="InParanoid" id="D5GKY4"/>
    <dbReference type="OMA" id="PLEMINT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015217">
        <property type="term" value="F:ADP transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015867">
        <property type="term" value="P:ATP transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052217">
        <property type="entry name" value="Mito/Peroxisomal_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45939:SF1">
        <property type="entry name" value="MITOCHONDRIAL THIAMINE PYROPHOSPHATE CARRIER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45939">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PMP34-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="133"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="14"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="130"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="223"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ85177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="340" mass="36592" checksum="D224AB573C20E8A0" modified="2010-06-15" version="1">MAGQSKETPLPSWGYALAGATGAVLANAAVYPLDIVKTKLQVQVQVKRKPKNTEKSLENGDVTRCDEEEGEIYTSAIDAIQKIIAKSGVNGLYTGMIGSLIGVASTNFAYFYWYTLVRNFYLSKSTTSALSTAVELSLGAVAGALAQLFTIPVAVVTTRQQTRPYSAEPLGLFATAQEVIGEDGVSGLWRGLKASLVLVVNPAITYGCYQRLKQILFNGRDRLSPGEAFLLGALSKSLATLATQPLIVAKVGLQSAPPPGRKAYKSFGEVMKVIVEKEGALGLFKGIGPQLVKGLLVQGVLMMSKERMELIFILLFRWFKKMRAKRLAKAAGTLPLVVKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>D5GL02</accession>
    <name>D5GL02_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00009857001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430342">
        <property type="protein sequence ID" value="CAZ85195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841004.1">
        <property type="nucleotide sequence ID" value="XM_002840958.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GL02"/>
    <dbReference type="STRING" id="656061.D5GL02"/>
    <dbReference type="GeneID" id="9185789"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009857001"/>
    <dbReference type="eggNOG" id="ENOG502SUQR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_480750_0_0_1"/>
    <dbReference type="InParanoid" id="D5GL02"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="15"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="567" mass="63270" checksum="F295D05DA77E9FA7" modified="2010-06-15" version="1">MDDKSPPFTHDRPPAKKPYLLPPEILSLIIQHLTPQDILSLSLTNHYHNAFTSTEAWRIYLQTHLPTAHTQILQEYHYQQQQQQHGPQYQGSKANWARWAEEATRVSRNFSRAGFVATALRPAIHMYVPRGPRRGPGGGQVNAWLWEPGTRGASGGRRQTVAYRPMVDSYSEFRGDRDVLAIGAGQDIMVSKGWGQRGAFWWGFENREERGGRDDVTGLHLLRPYEKAGNKDVEVALVGRANGRLEMLELDTRGGVGGTCRSKVVVRYHTDGRKVKGMDMMHEGNGGGVLMGAILNNPSLSIYNVRQPVGAGEPVRTANQASEVQFIDEQPWEVSFLDHTKVAVGKASTKPIAIHAITPSGLTQEPIREFSAKDCADMRSASVYVIKPLPRGSAGASANGDVFLSGCWDGIARQLPSPFSSLHRHFLTCTQAPRPPHPSTRNLPFLRSHRLPNTHLLSPSALQPHLCNRRKPIRPAKVLRYSLPLLLPLRLLAKKLGNIHPIPHRRRPGKPRALPRTWRWREDSVCWFARKGLGVGFLGKGRGEKGWCSRGVEYVFASRTNDLFHAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5GL11</accession>
    <name>D5GL11_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="12">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00009902001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. TCR/Tet family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430344">
        <property type="protein sequence ID" value="CAZ85204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841013.1">
        <property type="nucleotide sequence ID" value="XM_002840967.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GL11"/>
    <dbReference type="FunCoup" id="D5GL11">
        <property type="interactions" value="53"/>
    </dbReference>
    <dbReference type="GeneID" id="9185830"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009902001"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_22_1_1"/>
    <dbReference type="InParanoid" id="D5GL11"/>
    <dbReference type="OMA" id="GHEKWIG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17502">
        <property type="entry name" value="MFS_Azr1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000489">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000196">
        <property type="entry name" value="MFS toxin efflux pump (AflT)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1720.10:FF:000012">
        <property type="entry name" value="MFS toxin efflux pump (AflT)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF199">
        <property type="entry name" value="MFS EFFLUX TRANSPORTER INPD-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="77"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="172"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="205"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="237"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="277"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="308"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="349"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="382"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="412"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="436"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="475"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="548"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="11">
        <location>
            <begin position="82"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="50"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ85204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="581" mass="61199" checksum="C2C729FABE1C8951" modified="2010-06-15" version="1">MATTTDSSTLAPSVNNDVGGTGEKGFLDNSRPVSPNLDSEKRPITPTTHGEGVVSSDAGSEGGSEGSEDETQYPGPFALTLITIALSLAVFLVALDQTIIATAIPKITDQFGALNDVGWYGSAYMLTICSFQLVYGKIYTFFSLKWVFLAAIGIFELGSLVCGVAPTSKALIVGRAIAGLGCAGIFSGALIILAHSVPLRKRPMYTGFIGAMYGIASVAGPLMGGAFTDHATWRWCFYINLPIGAITVAVMILYFTPPKRAKADKLTLTEKLKQVDFVGTGLLLPGVVSLLLALQWGGTTYKWGSGRIIGLLVTAFVLLTGFVATQFWRQEKATIPPRIVKQRSIAFSSMLAFSVGSAFMLLVFYIPIWLQAIKSVSATKSGIMNLPLILGVVVLSILAGILITICGYYTPFMIVGASICAIGAGLLTTFEVDTGHAKWIGYQFICGAGIGLCMQQPMIAAQTVLKLEDVPTGTAVIVFFQTLGGALFISVGQNVFSNKLVAGLAESLPNIDPVAILNTGATSLKSTFPADMHPAILKAYNHALVECFYPAVAMAVVGVIASLGVEWASVKGKKVEAVAGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>D5GL45</accession>
    <name>D5GL45_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00009987001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430346">
        <property type="protein sequence ID" value="CAZ85238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841047.1">
        <property type="nucleotide sequence ID" value="XM_002841001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GL45"/>
    <dbReference type="FunCoup" id="D5GL45">
        <property type="interactions" value="734"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GL45"/>
    <dbReference type="GeneID" id="9185832"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009987001"/>
    <dbReference type="eggNOG" id="KOG1888">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003016_0_0_1"/>
    <dbReference type="InParanoid" id="D5GL45"/>
    <dbReference type="OMA" id="KRKCCAH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070772">
        <property type="term" value="C:PAS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043813">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043573">
        <property type="entry name" value="Fig4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002013">
        <property type="entry name" value="SAC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45738">
        <property type="entry name" value="POLYPHOSPHOINOSITIDE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45738:SF5">
        <property type="entry name" value="POLYPHOSPHOINOSITIDE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02383">
        <property type="entry name" value="Syja_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50275">
        <property type="entry name" value="SAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAC" evidence="5">
        <location>
            <begin position="270"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="22"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="800"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="840"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="27"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="70"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="86"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="808"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="847"/>
            <end position="856"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ85238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1015" mass="116105" checksum="DC183E5A77D01F1A" modified="2010-06-15" version="1">MNCEIPVLRRSEPSIISVKVFPKDEIASATTQPAVTTTPAPATADEAPATPSHPPSLSFRLEAPEPTYPQLLNNPPASPITNQPHRISDDKDEESSVPKSFDRSDFKHCHRDHKEFLHDDKRDFEGVRRMHKFTLYETATRFYIVGSDLLDSRFRILKIDRTAEIGDLSITEDEVVYTREETARLLATIEDGNISTGGLKQKCPFWGLLGFIRFTGAYYMLLITKRSIVAMVGGHYVYQIDNTELVPLTAGTAPKKPERNSEEARFVSILRNLDLTRSFYFSYSYDITRTLQHNIIRQREALGKGLANPNNHDYNDMFAWNHYLLEPAKSHMRNAYDWCMPIVHGYVDQAAISVYGRVIYITVIARRSRYFAGARFLKRGANDLGYVANDVETEQIVSEMLTTSFHAPMKNLYSNPNYTSYVQHRGSIPLFWTQKSDAATPKPPVEMNLVDPFFSAAALHFDDLFQRYGAPVIVLNLVKSRERTPRESLLLKEFTQAINYLNQFLPTEKSIRYIAWDMSRASKSRDQDVIETLETIAENVLNTTGFFHNGLKPGNESEGGYSLQNGVCRTNCIDCLDRTNAAQFVIGKRALGHQLHALGVISGTSIDYDTDAVNLFTHMYHDHGDTIAVQYAGSHLVNTMETYRKINQWTSHSRDMLESFKRYYNNSFLDGQRQEAYNLFLGNYIFAQGQPMLWDLSTDYYLHHADPRLGRRRRSYVKWWTPKNLVKRSMPEIPPLSGELTARPYSHFDDYWLEYYRPKALSSFLKVFTYNLNSTLRYIPVKMTQTGRYDLSPFTVRSNHVDHHHHHHDAENAEEKAKAERDSETAEGRLKKITALHGWLPPQNGIAPPPPPPPPLRKSSSISVHHQLELTRGLSSSTKQHFSLGKDVMQIERRDMNQAIERSLNPVLGEEEAEEYKRYMSHPLNLPLVVTLEPPEDVDPDSVYFDYVNNTSPEAAAGMTAKAEDVALYGEFVEVPENPLSVDETDREKKRYIAYQRWITTGNFKHLRSVGNIRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLM3</accession>
    <name>D5GLM3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00010270001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430349">
        <property type="protein sequence ID" value="CAZ85416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841225.1">
        <property type="nucleotide sequence ID" value="XM_002841179.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLM3"/>
    <dbReference type="STRING" id="656061.D5GLM3"/>
    <dbReference type="GeneID" id="9182719"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010270001"/>
    <dbReference type="eggNOG" id="ENOG502QYB8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_406627_0_0_1"/>
    <dbReference type="InParanoid" id="D5GLM3"/>
    <dbReference type="OMA" id="CETCQAS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16620">
        <property type="entry name" value="vRING-HC-C4C4_RBBP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="26"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="95"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="520"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="114"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="159"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="575"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="603"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="625"/>
            <end position="639"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ85416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="676" mass="72717" checksum="3E29EDAB44779B05" modified="2010-06-15" version="1">MATAHEQSMNASSAIPLPEIPVKLRCTICSELARGASRLPCCEQSICESCRSKLPDVCPVCDHSPLGASDCKPNGALRTTVAVFLRTAEKKHNLSMQKLQKEQAKPVEPQSEAKVLEPKPEKPQPTHASPVVNPLTPPQEASEVARSEGPLVPPPTVRESTEQPGEKSPGEKEATPVSDQQANGFVGDQNAWFGQSTAPMGGIPNMFSAHGVAGTPWGDYSMMQNMQGGWANGYGGMMGYTMGMNPMAMAQGGGFDGGSYGGMGMSRIGFNNGQGGGSMGWNNGWNGQNNVGFNPGIDGTRNGGFYSAASAGGYNHQSHGHHQMPQQYHNPHFQRQQPYQRGGRGGGFAGAGQRQVSLEHLQQNFQDAQFQQQIQGIDEAVAAVTTSGEKKGEKIQSVVSEDLDNIADAIDVPTDSASTVVVGGAIGTTEDLAESYNSITPVNPDMAAQYFPTDDFRSQGFQQQQVYPMQFNNGFRGSLRRGGFRGDFMGGPRGGGHVGPIPAAPGSMDGDRRPIEGIGLGVEGAPTGPKALRERGLSRSGPPIRAGYIGRRGGGYPVSSWTRSISPDHAFSRSRSQDSRRRSRSPDRGRSQERDRSRDRSRDRRRSIRSRSRHRKHRSPSVEVNGERPRGRSLDRDAADPPSATANPPETANDDGGEGGRRITYYHSLDFFLFRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLV4</accession>
    <name>D5GLV4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ATP-dependent Clp protease proteolytic subunit</fullName>
            <ecNumber evidence="7">3.4.21.92</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00010433001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the peptidase S14 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.92" evidence="7"/>
    <dbReference type="EMBL" id="FN430351">
        <property type="protein sequence ID" value="CAZ85521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841330.1">
        <property type="nucleotide sequence ID" value="XM_002841284.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLV4"/>
    <dbReference type="STRING" id="656061.D5GLV4"/>
    <dbReference type="MEROPS" id="S14.A02"/>
    <dbReference type="GeneID" id="9186505"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010433001"/>
    <dbReference type="eggNOG" id="KOG0840">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058707_3_1_1"/>
    <dbReference type="InParanoid" id="D5GLV4"/>
    <dbReference type="OMA" id="RDYWMKA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009368">
        <property type="term" value="C:endopeptidase Clp complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051117">
        <property type="term" value="F:ATPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07017">
        <property type="entry name" value="S14_ClpP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000001">
        <property type="entry name" value="ATP-dependent Clp protease proteolytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00444">
        <property type="entry name" value="ClpP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001907">
        <property type="entry name" value="ClpP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023562">
        <property type="entry name" value="ClpP/TepA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033135">
        <property type="entry name" value="ClpP_His_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018215">
        <property type="entry name" value="ClpP_Ser_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001368">
        <property type="entry name" value="PRK00277.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10381">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE PROTEOLYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10381:SF11">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE PROTEOLYTIC SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00574">
        <property type="entry name" value="CLP_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00127">
        <property type="entry name" value="CLPPROTEASEP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00382">
        <property type="entry name" value="CLP_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00381">
        <property type="entry name" value="CLP_PROTEASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0720">Serine protease</keyword>
    <feature type="active site" evidence="5">
        <location>
            <position position="136"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ85521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="242" mass="26612" checksum="74989DE8A7C689CA" modified="2010-06-15" version="1">MPLLSTSLRRLSRSTIPKLTPATTATRSFISTPPLKDYYTPTPFVHESVGGGWHSYDIFSRLLKERIICLNGPVHDGISAVVVAQLLFLEAENPEKQINLYINSPGGSVTAGLAIYDTMNYIQAPVSTICIGQAASMGSLLLCGGTKGKRYALPHASVMVHQPSILMTKGNSDIAIHAKEILRVRERLNIIYQKHLTKPHTLAQIEKIMERDLFMGAEEAKEMGLVDEILVARKKSSEVPSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLY2</accession>
    <name>D5GLY2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00010470001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430351">
        <property type="protein sequence ID" value="CAZ85549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841358.1">
        <property type="nucleotide sequence ID" value="XM_002841312.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLY2"/>
    <dbReference type="STRING" id="656061.D5GLY2"/>
    <dbReference type="GeneID" id="9187329"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010470001"/>
    <dbReference type="eggNOG" id="ENOG502RYFW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_654151_0_0_1"/>
    <dbReference type="InParanoid" id="D5GLY2"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39601">
        <property type="entry name" value="CHORIOGENIN HMINOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39601:SF2">
        <property type="entry name" value="CHORIOGENIN HMINOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="315"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="336"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="356"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="420" mass="46152" checksum="18B57A038D1F001E" modified="2010-06-15" version="1">MWPTTSAASPPARVAFDKFQKFLVKHYQARFGSWPPQTPDGRFTRMLYVQLSRDFAALYDYLVDREVCWSGPSLATFDPPGNGRHSIQPPSSTSGSRKIVKPGSPHWRADDDDLQMTDILLGFDDRHNYPHIPHPFPLVPPTMSPPAKLKLTSNASSAAGRFFQGAARRGSIPPHLPSNSTPFINSATEKAAALSLSESTNIESLLSASTSNSLVDAFAKHEKSIQPTEVDPHDARKGRWLLIYGILQTLATVSVDAPGLRWTDKVDYFLCAKLRGTPPWKGGTGAGVDGVDFGGNTSTDERSHFRSHCWTVPRSWRGVPAPPPPRTPTSSIASPLLPPSPPLHSPPLQMGIAELPHQRERERDGERESADDGDDEMGGMSDAPIGIERVDRVKKKESKKSFVENKVRWQDAWGTGPVSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLZ6</accession>
    <name>D5GLZ6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00010335001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430351">
        <property type="protein sequence ID" value="CAZ85458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLZ6"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010335001"/>
    <dbReference type="HOGENOM" id="CLU_2814294_0_0_1"/>
    <dbReference type="InParanoid" id="D5GLZ6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ85458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="67" mass="7333" checksum="6F1F1904D5858CB5" modified="2010-06-15" version="1">MIGQKRFRGRIYEFRGFSACTMLTPNGSQTGGCSGHELCLNGVRGVGKRCPRAHHDTIFLYKASSGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>D5GM17</accession>
    <name>D5GM17_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="15">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">GSTUM_00010484001</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NDC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430351">
        <property type="protein sequence ID" value="CAZ85560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841369.1">
        <property type="nucleotide sequence ID" value="XM_002841323.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GM17"/>
    <dbReference type="STRING" id="656061.D5GM17"/>
    <dbReference type="GeneID" id="9187340"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010484001"/>
    <dbReference type="eggNOG" id="ENOG502S1MG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_845170_0_0_1"/>
    <dbReference type="InParanoid" id="D5GM17"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070762">
        <property type="term" value="C:nuclear pore transmembrane ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106166">
        <property type="term" value="F:spindle pole body-nuclear membrane anchor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006999">
        <property type="term" value="P:nuclear pore organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070631">
        <property type="term" value="P:spindle pole body localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019049">
        <property type="entry name" value="Nucleoporin_prot_Ndc1/Nup"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13269">
        <property type="entry name" value="NUCLEOPORIN NDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13269:SF6">
        <property type="entry name" value="NUCLEOPORIN NDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09531">
        <property type="entry name" value="Ndc1_Nup"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="10" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="58"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="107"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="160"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="210"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="266"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="297"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAZ85560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="329" mass="37116" checksum="C81DAAA5EA08C743" modified="2010-06-15" version="1">MVSSISRSIPSSAPPRPPPPHYQTFLTPILHRRFARACLVGFAACYVESFVISNKSSFFWAIFPLGWTGFKAVILFFLSVFPILILRISQLHVGARSHTTVFHAMKTYIGSFSTYSTFLTHSFASLVFAFLYLWSSSKEDRLGFIIEGKSYERPRLNERFLYLTFFACYTGLVQAVLHLYEDRGRLQLPHLYLSPKAAFKKKFIEVPSGAFHMALISASTAPFAYMPFRPVIWHYTLVTAKTFYWLNRSSTLPSFPVGAGMFIRSLWLSFLIGAMWQISNIAFDVYFTQKPLSADGKTVSEKSSDPNGTLVTGLKASQAPLEPSNISID</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMA3</accession>
    <name>D5GMA3_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">DNA polymerase</fullName>
            <ecNumber evidence="20">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="ORF">GSTUM_00010623001</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27 28">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 20">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="19">Forms DNA polymerase zeta with REV7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 20">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 20">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="20"/>
    <dbReference type="EMBL" id="FN430354">
        <property type="protein sequence ID" value="CAZ85646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841455.1">
        <property type="nucleotide sequence ID" value="XM_002841409.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5GMA3">
        <property type="interactions" value="660"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GMA3"/>
    <dbReference type="GeneID" id="9183225"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010623001"/>
    <dbReference type="eggNOG" id="KOG0968">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000203_3_1_1"/>
    <dbReference type="InParanoid" id="D5GMA3"/>
    <dbReference type="OMA" id="GRNKMGF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016035">
        <property type="term" value="C:zeta DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042276">
        <property type="term" value="P:error-prone translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05778">
        <property type="entry name" value="DNA_polB_zeta_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05534">
        <property type="entry name" value="POLBc_zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.60:FF:000007">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.690:FF:000002">
        <property type="entry name" value="DNA polymerase zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000024">
        <property type="entry name" value="DNA polymerase zeta catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="entry name" value="DNA polymerase family B, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.342.10">
        <property type="entry name" value="DNA Polymerase, chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.690">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017964">
        <property type="entry name" value="DNA-dir_DNA_pol_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006133">
        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006134">
        <property type="entry name" value="DNA-dir_DNA_pol_B_multi_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056435">
        <property type="entry name" value="DPOD/Z_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030559">
        <property type="entry name" value="PolZ_Rev3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056447">
        <property type="entry name" value="REV3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025687">
        <property type="entry name" value="Znf-C4pol"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45812">
        <property type="entry name" value="DNA POLYMERASE ZETA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45812:SF1">
        <property type="entry name" value="DNA POLYMERASE ZETA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00136">
        <property type="entry name" value="DNA_pol_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24055">
        <property type="entry name" value="POL3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24065">
        <property type="entry name" value="REV3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14260">
        <property type="entry name" value="zf-C4pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00106">
        <property type="entry name" value="DNAPOLB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00116">
        <property type="entry name" value="DNA_POLYMERASE_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 20" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="16" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7 20" id="KW-0235">DNA replication</keyword>
    <keyword evidence="15 20" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="12 20" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="13 20" id="KW-0408">Iron</keyword>
    <keyword evidence="14 20" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8 20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 20" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="17 20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="11 20" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 20" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNA polymerase zeta catalytic subunit N-terminal" evidence="26">
        <location>
            <begin position="3"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="DNA polymerase delta/zeta catalytic subunit N-terminal" evidence="25">
        <location>
            <begin position="57"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B exonuclease" evidence="23">
        <location>
            <begin position="781"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B multifunctional" evidence="22">
        <location>
            <begin position="1050"/>
            <end position="1463"/>
        </location>
    </feature>
    <feature type="domain" description="C4-type zinc-finger of DNA polymerase delta" evidence="24">
        <location>
            <begin position="1521"/>
            <end position="1593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="267"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="509"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="272"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="509"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="549"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="568"/>
            <end position="582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00066055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU000442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF00136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF03104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF14260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF24055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF24065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EMBL" id="CAZ85646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1622" mass="183876" checksum="75448B17285BBBE6" modified="2010-06-15" version="1">MAFRIRLNNIDSYQAYPTSLDPTFPHIPDDERPPKIPIVRVFGATETGQKVCAHIHGAFPYLYVEYPGKSLMPEDDRAMGLSAGRSIRSQEKPPRFVAHISLVKGVPFYGFHVGWKFYCKIYLFNPHMRGRLARILQSGGVLSRVFQPHEAHIQYIPQFMIDFNLNGCGFIECDKVLFRGEIPDADEIGEQHIWHNKSIPEVSILPESQFQRQSYCTLEVDIHVRDIFNRKQVSARPLHFDFVERTNPISPDIKLVHSMAELWKDENRRRGNNSNPAEGLVSSSSDQRGPSGTWIHEEEYRERIKDIIKTERRVSDGRTLKFETYVSRAQFENLWPTAFESVAESFEGAGLPPDKKVELVVEAEIAEVDESLVYDIGEGEFSDSDFEGLFDVMDDPGGEQSAKVKAEKSHEQVKRGLVWELQGRELKISSSPPMPEASNGEGRVEKSLSNNSQLTIAGGSFYEGLVEEFIEEEDDIVVTDSRVWFPTSSVDPDVPTSYQVPSKRVKMESFSSNIESQMPPSSDCSDVPEKICSTSASVRRDIPNGKSPALSNGHPSSAQPGCRFVDSVSENSKTRASISEPLQKSRKRAQPSSSRANDTPRPEKIGRRGKVAVDLSNLPTRLDPTSSLRGAFDIPSNSSIRCYSQRAPSSSELLTSEDKEEIAKVAYQEPYYSDDRDVPTKVWEYAGREFKFKSSKLSDLPIFNPNNENRIVWEYNRLNQRDPRFKMWNIAEAPPSRKEVAKLLEERKIVRKGKGARQSVAYEHNSFFASQIQGPTQKNKYGFKFSQNAKSDNVQHELQHMSIMSLELHINTVGDFVPNAERDQIECIFWSLKEMEEGEDGEVKEVTRIGIITLVDEHNNDPNPGLKFRRQCRAEIRTETSELGMINTLIDTVRVLDPDILTGWEVNSSSWGYLIKRANQLFEMNLCDDLSRVKIETHSRFGKESNLWGFNTGSAIRITGRHMVNIWRAMTAELNLLGYSMENVVFHLLHQRIPHYSFRVLTGWYTSGSPLLFGKVIKHLVRKVQLDLEILDRQELITRTSEQARLLGIDFASVSGRGSQFKVESMMFRIAKPESFILVSPSKAQVGQQNALECLPLVMEPFSRFYTSPVVVLDFQSLYPSVMIAHNYCYSTFLELCEKDINIAPNGMMYIKQTIRKSLLAKMLTEILDTRVMVKNGMKRDKHDKALQKLLNSRQLALKLIANVTYGYTSASHSGRMPCTEIADSIVQTGREILEKAIRLIQDDPNWDAEVVYGDTDSLFIHLEGRTKDEAFRIGEEIAARVTEANPRPIKLKFEKVYLPCVLLAKKRYVGFKYEYREQAEPDFDAKGIETVRRDGTPAEQMIEEAALKILFRTKDLSQVKAYCQREWTKIMREKAVRLGTYAEGVSLPPGAQLAAKRVEKDPENAPQYAERIPYVVIAGSPGQTLADRTIEVNDLLASPELSLDSEYYICKNIIPPLERIFNLVGANVRQWYDEMPKYKHLRKLIKYNDSAAATSGGTGAQKEKSRAIIESYMTKSSNICAVCGCKTSRTKNNICKDCTQNRDASIIHVRTQLARAEKRVLDLQTVCRSCEGSSPGEEVACTSQDCAVYYSRRRQGAAFTWERENVRGVLGVLEGVGELEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMI5</accession>
    <name>D5GMI5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00010744001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS12 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430356">
        <property type="protein sequence ID" value="CAZ85728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841537.1">
        <property type="nucleotide sequence ID" value="XM_002841491.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMI5"/>
    <dbReference type="FunCoup" id="D5GMI5">
        <property type="interactions" value="334"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GMI5"/>
    <dbReference type="GeneID" id="9182154"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010744001"/>
    <dbReference type="eggNOG" id="KOG1750">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104295_0_1_1"/>
    <dbReference type="InParanoid" id="D5GMI5"/>
    <dbReference type="OMA" id="VCIRVYT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03368">
        <property type="entry name" value="Ribosomal_S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000099">
        <property type="entry name" value="Ribosomal protein S12, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006032">
        <property type="entry name" value="Ribosomal_uS12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005679">
        <property type="entry name" value="Ribosomal_uS12_bac"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00981">
        <property type="entry name" value="rpsL_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11652">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S12 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00164">
        <property type="entry name" value="Ribosom_S12_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01034">
        <property type="entry name" value="RIBOSOMALS12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00055">
        <property type="entry name" value="RIBOSOMAL_S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="135"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="149"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ85728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="158" mass="17381" checksum="E8F08C49076E96DB" modified="2010-06-15" version="1">MFTLHPLWRFLPRTATAAAPRTFTATSHPAATFNQVRRGCRKPQPKRKPESPAMSETHNPCMKGVCLKVFVMAPKKPNSAERKVARVRLSSDKVVTAYIPGEGHNAQQHSVVLVRGGRSQDCPGVKYHLIRGALDLGGVPNRSTSRSKYGTKKPKKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMI6</accession>
    <name>D5GMI6_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Sensitive to high expression protein 9, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00010745001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Required for the maintenance of the structure of the mitochondrial inner membrane. Involved in mitochondrial morphology. Causes growth arrest when highly overexpressed.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homooligomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Mitochondrion inner membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the SHE9 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430356">
        <property type="protein sequence ID" value="CAZ85729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841538.1">
        <property type="nucleotide sequence ID" value="XM_002841492.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMI6"/>
    <dbReference type="GeneID" id="9182155"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010745001"/>
    <dbReference type="eggNOG" id="ENOG502QQ1E">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025632_2_1_1"/>
    <dbReference type="InParanoid" id="D5GMI6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007007">
        <property type="term" value="P:inner mitochondrial membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008839">
        <property type="entry name" value="MDM33_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31961">
        <property type="entry name" value="SENSITIVE TO HIGH EXPRESSION PROTEIN 9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31961:SF3">
        <property type="entry name" value="SENSITIVE TO HIGH EXPRESSION PROTEIN 9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05546">
        <property type="entry name" value="She9_MDM33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 10" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="257"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="419"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="59"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="138"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="66"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ85729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="444" mass="49101" checksum="86063BA662496B97" modified="2010-06-15" version="1">MRPLLRSILLARPSGSPAACRILDLRRLHLSPRLSLCIRCQPHLQQQPLRLPQQHFRWFSSEGKPPDSGNKGDSPSPDSSGTPSSTPSPQSELRDLPSQMENRRLNMHRKFSAAMDDLQAAMSRAGQKLNEITGYSDIEALKRSIEQREQHVKASRQAVRNAKEEYQAAISRRSASQREVNELLQRKHAWSPTDLERFTELYRSDHANEQAETTAQDLLVQAERVADEAQTGLARSILSRYHEEQIWSDKIRRASTWGTWGLMGFNVLLFIVVQLGLEPWKRKRLVGGFEEKVREVIQEEGLRSALLRAPPVGERGGPSGAAQFVADKTAAVGGEVAEAADSLEVVAAVGEAPPAAAEVADATEAITGENIAAIEVAAEAPSEPVVDERPWIDYIKEEVTGKVEELFGEEVVAIKRVDLTITILESVAVGTFIMATLSLLWSSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMM5</accession>
    <name>D5GMM5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00010813001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I NDUFA5 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430357">
        <property type="protein sequence ID" value="CAZ85768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841577.1">
        <property type="nucleotide sequence ID" value="XM_002841531.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMM5"/>
    <dbReference type="STRING" id="656061.D5GMM5"/>
    <dbReference type="GeneID" id="9185104"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010813001"/>
    <dbReference type="eggNOG" id="ENOG502S4WS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084284_0_0_1"/>
    <dbReference type="InParanoid" id="D5GMM5"/>
    <dbReference type="OMA" id="WTYFERG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006806">
        <property type="entry name" value="NDUFA5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12653:SF0">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12653">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 13 KD-B SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04716">
        <property type="entry name" value="ETC_C1_NDUFA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ85768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="234" mass="25972" checksum="D02F6D84FC2EE9B1" modified="2010-06-15" version="1">MLPTLRLLAQVGTQRLAPFSTTGIAGILTHPNPRPALIAVYNHTISLLSRLPQHSVYRQSTENFTKHRLAIVESVKPEGWEGYQAALKSEREANGIRGKKDTEFELKVVGKQFSVMANRVTTDSPVIQAFLDKEVGRWGLDPAVDTSAAYVGDDVPVEQKGSSSEVPLLPEEPPLHAEQVAELEEKIGAGLIEEVLEQGWNELNLIKEMEEAKVWESLEVQPEEGQWVGFERTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMY9</accession>
    <name>D5GMY9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00011031001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430361">
        <property type="protein sequence ID" value="CAZ85902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841711.1">
        <property type="nucleotide sequence ID" value="XM_002841665.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMY9"/>
    <dbReference type="FunCoup" id="D5GMY9">
        <property type="interactions" value="807"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GMY9"/>
    <dbReference type="GeneID" id="9183470"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011031001"/>
    <dbReference type="eggNOG" id="KOG2963">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026936_2_0_1"/>
    <dbReference type="InParanoid" id="D5GMY9"/>
    <dbReference type="OMA" id="KDYTVMT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007109">
        <property type="entry name" value="Brix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045112">
        <property type="entry name" value="PPAN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12661">
        <property type="entry name" value="PETER PAN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12661:SF5">
        <property type="entry name" value="SUPPRESSOR OF SWI4 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04427">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00879">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50833">
        <property type="entry name" value="BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Brix" evidence="2">
        <location>
            <begin position="72"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="333"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="391"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="391"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="440"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ85902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="473" mass="52678" checksum="D1244B040A6971DC" modified="2010-06-15" version="1">MGTDLVLGLSSCPIFRNFILSTTTNRHPPTIKHRMARRRTKKRTHITPVDPTKSATVGGQPLNASASRTPKTMVIRVGASEVGPSISELVRDVRAMMEPYTASRLRERKSNKLKDYTTMAGPLGVTQLLLFSRNAGSGSATLRIARCPRGPTIHFRVKNYSLCRDIRKSMRNPKTPGKEFASPPLLVMNNFTTPQPPNADGTPGRPPPHEVLLTSMFQSLFPPISAQRTPLSSIRRVLLLDRQLMPSDSGDAPSEYVIEARHYIISTKAVGLSKPIRRLDAAQRAVRQPKTSRRGALPNLGKLEDIADYMLDPGAAGGYTSESEVEEDAEVEVLQSASSKQAPRKKRTPKGPEKRVVKLSEIGPRMKLEMVKIEEGLAEGKVLWHAWEKKTKAEERELEIRHKQKKEERDRRRREQMENVKRKKAEKAAAGEKKGGGDGGGDEEMWDDEYDGEEESEEGSGDEEGEDEKMGDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>D5GN50</accession>
    <name>D5GN50_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00011091001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430362">
        <property type="protein sequence ID" value="CAZ85943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841752.1">
        <property type="nucleotide sequence ID" value="XM_002841706.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GN50"/>
    <dbReference type="GeneID" id="9185869"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011091001"/>
    <dbReference type="HOGENOM" id="CLU_2795796_0_0_1"/>
    <dbReference type="InParanoid" id="D5GN50"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ85943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="68" mass="7996" checksum="F7AC5654C7E10D51" modified="2010-06-15" version="1">MRSKPLHKALTRKTLKASHHHQKERKHYRSDFFFCIIASTLLSNLMRWRILAGRPHLSLLSPRFGGTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNX1</accession>
    <name>D5GNX1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosome production factor 2 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Ribosome biogenesis protein RPF2 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00011560001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
            <location evidence="1 4">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the RPF2 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430371">
        <property type="protein sequence ID" value="CAZ86214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842023.1">
        <property type="nucleotide sequence ID" value="XM_002841977.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNX1"/>
    <dbReference type="FunCoup" id="D5GNX1">
        <property type="interactions" value="926"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GNX1"/>
    <dbReference type="GeneID" id="9183259"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011560001"/>
    <dbReference type="eggNOG" id="KOG3031">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049783_0_0_1"/>
    <dbReference type="InParanoid" id="D5GNX1"/>
    <dbReference type="OMA" id="VGLKPMF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007109">
        <property type="entry name" value="Brix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039770">
        <property type="entry name" value="Rpf2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12728">
        <property type="entry name" value="BRIX DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12728:SF0">
        <property type="entry name" value="RIBOSOME PRODUCTION FACTOR 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04427">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00879">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50833">
        <property type="entry name" value="BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Brix" evidence="6">
        <location>
            <begin position="28"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="270"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="283"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="297"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ86214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="319" mass="36288" checksum="C6B3B9ED9C55334E" modified="2010-06-15" version="1">MIRTTKPRNARSKRALEARECKLVENQKSALFLSGTSTSDLTRSAMTDIHSLKKPNAIKFTKKNSIHPFEDATPLEFFSSKNDTSFLVFASHSKKRPHNLTIARTFDHRILDMLELGIDESTFRPLTFFKTSKPGIGMKPLLSFSGAIFDSIPNYRLFKSMFLDFFRGETVNSVNVEGLQYMIHFSAAESTEQNPAPAIHMRVHLIKTLKSGQKLPRVEVEEMGPRIDFRIRRVHEPDEAMMKEALKKPRALEARSKKNIETDIMGDKIGRIHTGGQDFSKMQSRKMKGLKRRPTDEADEEMTLVDEDEDGGAKKARLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>D5GP05</accession>
    <name>D5GP05_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 17">Homoaconitase, mitochondrial</fullName>
            <ecNumber evidence="5 17">4.2.1.36</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16 17">Homoaconitate hydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">GSTUM_00011608001</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 17">Catalyzes the reversible hydration of cis-homoaconitate to (2R,3S)-homoisocitrate, a step in the alpha-aminoadipate pathway for lysine biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 17">
            <text>(2R,3S)-homoisocitrate = cis-homoaconitate + H2O</text>
            <dbReference type="Rhea" id="RHEA:15485"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15404"/>
            <dbReference type="ChEBI" id="CHEBI:58174"/>
            <dbReference type="EC" id="4.2.1.36"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="17">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 17">Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-alpha-aminoadipate from 2-oxoglutarate: step 3/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 17">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the aconitase/IPM isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.36" evidence="5 17"/>
    <dbReference type="EMBL" id="FN430371">
        <property type="protein sequence ID" value="CAZ86248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842057.1">
        <property type="nucleotide sequence ID" value="XM_002842011.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GP05"/>
    <dbReference type="FunCoup" id="D5GP05">
        <property type="interactions" value="122"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GP05"/>
    <dbReference type="GeneID" id="9183541"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011608001"/>
    <dbReference type="eggNOG" id="KOG0453">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006714_3_1_1"/>
    <dbReference type="InParanoid" id="D5GP05"/>
    <dbReference type="OMA" id="LCDADNI"/>
    <dbReference type="UniPathway" id="UPA00033">
        <property type="reaction ID" value="UER01027"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004409">
        <property type="term" value="F:homoaconitate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01674">
        <property type="entry name" value="Homoaconitase_Swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000013">
        <property type="entry name" value="Homoaconitase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="entry name" value="Aconitase, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="entry name" value="Aconitase, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004418">
        <property type="entry name" value="Homoaconitase_mito"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039386">
        <property type="entry name" value="Homoaconitase_swivel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050067">
        <property type="entry name" value="IPM_dehydratase_rel_enz"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00139">
        <property type="entry name" value="h_aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43822:SF2">
        <property type="entry name" value="HOMOACONITASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43822">
        <property type="entry name" value="HOMOACONITASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="Aconitase iron-sulfur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 17" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10 17" id="KW-0408">Iron</keyword>
    <keyword evidence="11 17" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="14 17" id="KW-0456">Lyase</keyword>
    <keyword evidence="13 17" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="8 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 17" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 17" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha" evidence="19">
        <location>
            <begin position="73"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase A/isopropylmalate dehydratase small subunit swivel" evidence="20">
        <location>
            <begin position="524"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="502"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00021560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00029338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00032706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU362038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="CAZ86248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="716" mass="77899" checksum="184AE98619C80991" modified="2010-06-15" version="1">MHLLRVPASRAYIRLSACRALPPRIPSYFQPPTSFSLTRSYANISPSPTASQNLTEKIVQKYAVGLPVGKFLRSGDYVSIKPHHCMTHDNSWPVAQKFFSIGATRIHDNRQVVMTLDHDVQNKSETNLRKYQNIEELAKRMGVDFYGAGRGIGHQILVEEGYAWPGTLTVASDSHSNMYGGISCLGTPIVRTDAAAIWATGQTWWQIPPVARVELKGVLPKGVTGKDVIVSLCGLFNNDEVLNHAIEFVGSEQTLESLKIDDRLTIANMTTEWGALSGIFPMDDVLKDWMLQKASMHAQRLRNSSHPRLTNKKVEEFFALNTLLASPDASYAKSLTLDLSTLSPYVAGPNSVKISTPLTELEEKKIAIQKAYLVSCTNSRASDIAAAAEVFKSNPSAKVASGVDFYIAAASTLEQEAATKAGDWDALINAGAKALPAGCGPCIGLGTGLLEDGEVGISATNRNFKGRMGSKEALAYLASPEVVAASAMKGFIAGPGNYTGPSSAVSISSPPPPALPSTQSSSAQEAETEILPGFPPLIEGEIIFCDADNINTDGIYPGKYTYQDNISREQMAQVCMENYDSTFSNISRPNDILVTGFNFGCGSSREQAATAILARSIQLVVAGSFGNIFMRNSINNSLMTVELPKLIERLREKFPKTLTRRTGWWLQWDVRRSKVIVREGPEDAERWSLGVGELPPNVQDVFVKGGLEKWVKARLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GP36</accession>
    <name>D5GP36_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00011659001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CAND family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430372">
        <property type="protein sequence ID" value="CAZ86279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842088.1">
        <property type="nucleotide sequence ID" value="XM_002842042.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GP36"/>
    <dbReference type="STRING" id="656061.D5GP36"/>
    <dbReference type="GeneID" id="9186518"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011659001"/>
    <dbReference type="eggNOG" id="KOG1824">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007157_0_0_1"/>
    <dbReference type="InParanoid" id="D5GP36"/>
    <dbReference type="OMA" id="AYIPHFQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010265">
        <property type="term" value="P:SCF complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039852">
        <property type="entry name" value="CAND1/CAND2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013932">
        <property type="entry name" value="TATA-bd_TIP120"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12696">
        <property type="entry name" value="TIP120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08623">
        <property type="entry name" value="TIP120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25782">
        <property type="entry name" value="TPR_CAND1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="TATA-binding protein interacting (TIP20)" evidence="5">
        <location>
            <begin position="1042"/>
            <end position="1205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="327"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="330"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="346"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ86279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1222" mass="133235" checksum="3F4E673FC60322F7" modified="2010-06-15" version="1">MANASTPFALQSLLEKMSSPDSDYRFMSLNDLLSILTSPNASLTQADSNLTSRVIDGVVKALDDANGEVQNLAVKCLGPLVLKVRDNQIAPLIDRLTTLSMTSTDPSIPSTALRTIISSLPRPKTLYPKLLILLKPGTETVKGGGASLDSIDLLIETLKCFGPVLGGKEVEQLQVAVMELLESDRTSQVVKKRAVTSLSLLCVYSPDELLSSFINHLIESFRAQGKHVNPTRLRLLVSVTGALARDIPDRFGPYLKTLCPFILSVVDGKDIQDRELGDEPEMEMDEVREAALVSLESFMSFCTSNMTRFTEDVLNAGMIYLKYDPNYADPADEEDDEEMGGTQQYDGDEDDDFGGSDDEDAFEEDGNFSDEDDISWKVRRCAAKLLSTVLSTRATDLILGEQEGGGKAYKQVAPLLVERFHEREENVRLEVLATATVLVRKTGEVAQGTFTPSTPSRDRRGSMDINSLQALMPRLSKSLSKLLKTKSTTLPTKQASSSLRIEVLRLVGKICENHPAQVVGEHFDTLIPAMITAVGEPAYKISSEALNTVVSIVRLLTAEGSTGYSEYLKGLYDVIVAKVGNGDADLEVRERAIAALGVLLSRTSGQPDLIGPNRRAAALGMLVERAKNETTRITAVRAIEVIARNAKTTGDVTPDWVKTVVVECGIQLRKANRTLRGASLDALRSISANENCRKAMDPQSKRDLVDVLTTLLPAGDMHLLAPTLTIIRWMMIDGDGVYVTPGINEGVCGLVRNNLGSGLVVESLLGLVKAIGENDPVGKKTVMNGLLQDVGVNGETSIVAKVVAQLLVSGGGKRGGFAIGVEEFVGEVVKTTDDKRKCLALMVLGEIGLRMGPSFSVGPETFLQQFHAKSDDVPIAAAVALGLASAGNVQGYLPVIMQRLGGGDKDQYLLLHSLKEIIQHSDDTTANIKPYADQIWKALFSMAKNDDSKAVGAECVGRLTIIDPYSYLPELQKHLQSESSAIRGMVISALRYTFTDTEASYDDLLRPIVVDFLTVMVDDKELENRRLALTALNSAAHNKPHLIGQHLQSLLPLVYRETVVRPELVREVQMGPFKHKVDDGLESAYETLYALLETSFSGIEIQTYFDRVIAGLQDEHDIRVLCNLMLTKLILLAKGETLRRLDSIAECFKTTLNQKPKDNAVKQELEKHAESIRSTMRATVALNRTAIGAGVSGKAGWSGYWEWAKAVFPNELQVLEGDERAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPE3</accession>
    <name>D5GPE3_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">UDP-N-acetylglucosamine transferase subunit ALG14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">Asparagine-linked glycosylation protein 14</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ALG14</name>
        <name evidence="13" type="ORF">GSTUM_00011693001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Involved in protein N-glycosylation. Essential for the second step of the dolichol-linked oligosaccharide pathway. Anchors the catalytic subunit ALG13 to the ER.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 11">Heterodimer with ALG13 to form a functional enzyme.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Endoplasmic reticulum membrane</location>
            <topology evidence="1 11">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the ALG14 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430373">
        <property type="protein sequence ID" value="CAZ86305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842114.1">
        <property type="nucleotide sequence ID" value="XM_002842068.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPE3"/>
    <dbReference type="FunCoup" id="D5GPE3">
        <property type="interactions" value="283"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GPE3"/>
    <dbReference type="GeneID" id="9186545"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011693001"/>
    <dbReference type="eggNOG" id="KOG3339">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064541_0_1_1"/>
    <dbReference type="InParanoid" id="D5GPE3"/>
    <dbReference type="OMA" id="CRIVFIE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043541">
        <property type="term" value="C:UDP-N-acetylglucosamine transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004577">
        <property type="term" value="F:N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013969">
        <property type="entry name" value="Oligosacch_biosynth_Alg14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12154">
        <property type="entry name" value="GLYCOSYL TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12154:SF4">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE TRANSFERASE SUBUNIT ALG14 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08660">
        <property type="entry name" value="Alg14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072179" description="UDP-N-acetylglucosamine transferase subunit ALG14" evidence="12">
        <location>
            <begin position="31"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ86305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="223" mass="24439" checksum="5010AFF27A95D11A" modified="2010-06-15" version="1">MISLFLTGMIILSFLLFLRLLSVTPPITRTQGPSPPCSPSTRKPHILYLLGSGGHTAEMLSLISSPCPSASSSRYKRTYMISRGDALSVKKAAAFEYASTSSPTSYDILEIPRARLVGQSWLSTPWTCAACLAGCVRAFARVGFPDVVVCNGPGSAVVVVGVCFFCKLLGLCRTRIIYVESFARVRSLSLSGRLLYVFTDRFIVQWPGLVEKYRRAEYHGILI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPS8</accession>
    <name>D5GPS8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00012012001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430378">
        <property type="protein sequence ID" value="CAZ86521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842330.1">
        <property type="nucleotide sequence ID" value="XM_002842284.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPS8"/>
    <dbReference type="GeneID" id="9182265"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012012001"/>
    <dbReference type="HOGENOM" id="CLU_960401_0_0_1"/>
    <dbReference type="InParanoid" id="D5GPS8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ86521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="290" mass="32326" checksum="240C141A523F36DC" modified="2010-06-15" version="1">MSDDLLSHHHLISYPSFIPLSNNPTWYTTQLPKDRSKNRTYLFLPTPTSVSSFKHRIPKFLAINPNWSIYSFDKSILELYTKATRTSQLYEMRMCGGSQRRGHTGGENSIGRVVEDGEWFRETYKDEATLVDEEDVLSVVDPSGVRLAFTTCLGDSGRLLVTKVEVAEGVKQSVRGVEVVRKLLGTVADIAERGGLRAWINIPVEDAVIVGVLFEMGFVVNRTLWLVEGDAEVVDESGISDIVVGSGRVKSVMEKKNKAEVWKLPDWEGGMSGWREQWSAALDRIMAGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPV0</accession>
    <name>D5GPV0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00012043001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430378">
        <property type="protein sequence ID" value="CAZ86543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842352.1">
        <property type="nucleotide sequence ID" value="XM_002842306.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPV0"/>
    <dbReference type="STRING" id="656061.D5GPV0"/>
    <dbReference type="GeneID" id="9182287"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012043001"/>
    <dbReference type="eggNOG" id="KOG0086">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_6_0_1"/>
    <dbReference type="InParanoid" id="D5GPV0"/>
    <dbReference type="OMA" id="ASQNICI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007033">
        <property type="term" value="P:vacuole organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002488">
        <property type="entry name" value="Ras family GTPase (Rab4b), putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ86543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="268" mass="29351" checksum="19B9DC91359AD53E" modified="2010-06-15" version="1">MPRQRAASGASTESSRSAPEDQELGSLYDYLAKIILLGPSGCGKSCLLHRFVRGEWRILSSQTIGVEFSSKIIKVGTGSRRKRIKLQLWDTAGQERFRSVTRSYYRGAAGAVLIYDITSHPSFTSLPTFLTDARALASPNLTLLLVGNKQQVEVVERAVTTNEAARWASLQDIPVTMETSALSGENVDEVFTRLAHMIVTKIELGEVDPDDPMSGIQYGDSGGWVDASGSTRKRKGPGRVMADGFSAGLREWEDVFRDSSGGRRRKCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GQ48</accession>
    <name>D5GQ48_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00012197001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430382">
        <property type="protein sequence ID" value="CAZ86641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842450.1">
        <property type="nucleotide sequence ID" value="XM_002842404.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GQ48"/>
    <dbReference type="GeneID" id="9185221"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012197001"/>
    <dbReference type="HOGENOM" id="CLU_2795798_0_0_1"/>
    <dbReference type="InParanoid" id="D5GQ48"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ86641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="68" mass="8428" checksum="0B4AE1C62D1C052A" modified="2010-06-15" version="1">MDYSEPPRNYPHSRSSTCKFARTLHVTRKLVQLRLYNKQKRGENLVHSQLPWYHIQRHHTRMTQYRYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>D5G3Y7</accession>
    <name>D5G3Y7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00003861001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835109.1">
        <property type="nucleotide sequence ID" value="XM_002835063.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G3Y7"/>
    <dbReference type="STRING" id="656061.D5G3Y7"/>
    <dbReference type="GeneID" id="9187168"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003861001"/>
    <dbReference type="eggNOG" id="ENOG502S8U7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044363_0_0_1"/>
    <dbReference type="InParanoid" id="D5G3Y7"/>
    <dbReference type="OMA" id="QYATTFD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005640">
        <property type="term" value="C:nuclear outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070762">
        <property type="term" value="C:nuclear pore transmembrane ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030474">
        <property type="term" value="P:spindle pole body duplication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012578">
        <property type="entry name" value="Nucl_pore_cmplx"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28003">
        <property type="entry name" value="NUCLEOPORIN POM34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28003:SF1">
        <property type="entry name" value="NUCLEOPORIN POM34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08058">
        <property type="entry name" value="NPCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="46"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="201"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ79230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="258" mass="27495" checksum="9CC9667EB9E872E1" modified="2010-06-15" version="1">MSSAPSTPMKSAPATPATTGSWRHPALDDIAKRNANKRPDMSFKRLTMNAFALIASFAMFTFLRKSEFLLGLTPSSPTFHQYFSYVLWSARCVLAFNVGESITQLLAPADEFTDISLTPEQRKLLGLNPNTPLSASASMGNIVTPPRYTKSTPSSRTGSPSFGASTSGSPTLQRKSAPQTPPPMTPSPLRMGGGLERRQGNGIGSPSGGPFSGGSPGVKLAGASVVPGNKWLYEKNIQRKNEGLRFSPSKSIFSTPRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>D5G441</accession>
    <name>D5G441_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00003940001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835163.1">
        <property type="nucleotide sequence ID" value="XM_002835117.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G441"/>
    <dbReference type="STRING" id="656061.D5G441"/>
    <dbReference type="GeneID" id="9182411"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003940001"/>
    <dbReference type="eggNOG" id="ENOG502RUZC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1152457_0_0_1"/>
    <dbReference type="InParanoid" id="D5G441"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13565">
        <property type="entry name" value="HTH_32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="222"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="241" mass="26438" checksum="370F22CB7B5EC3D6" modified="2010-06-15" version="1">MSPPTSAEQRAKIEALLADGKIPRKDIGKHVDPPVCYGTVVRIQRNLKKYGTTNKPRQGVVGRPAKITEPMAQAVLEALTEKPEMYLEELARVLHDEFDEWVSVATLSRYLKKEGWVKEKDGENGAPPPHPTTVGSVGAGAGGSRATETESVTTDEGQDEMDQAVAAITPSPPLPMTPELQQRLQKLNNSAMRFFSATLNKDPTADLSPLFEKYRTLYPARRKRAQEKSTATGTQLITATQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5G471</accession>
    <name>D5G471_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00003974001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835193.1">
        <property type="nucleotide sequence ID" value="XM_002835147.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G471"/>
    <dbReference type="STRING" id="656061.D5G471"/>
    <dbReference type="GeneID" id="9184290"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003974001"/>
    <dbReference type="eggNOG" id="KOG3271">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021655_0_1_1"/>
    <dbReference type="InParanoid" id="D5G471"/>
    <dbReference type="OMA" id="HEEPCVV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045901">
        <property type="term" value="P:positive regulation of translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045905">
        <property type="term" value="P:positive regulation of translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001884">
        <property type="entry name" value="IF5A-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048670">
        <property type="entry name" value="IF5A-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020189">
        <property type="entry name" value="IF5A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11673">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR 5A FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01287">
        <property type="entry name" value="eIF-5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21485">
        <property type="entry name" value="IF5A-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Translation initiation factor 5A-like N-terminal" evidence="2">
        <location>
            <begin position="51"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Translation initiation factor 5A C-terminal" evidence="1">
        <location>
            <begin position="124"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ79314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="198" mass="21962" checksum="F38BB9424673A603" modified="2010-06-15" version="1">MVKYDAYSTSAHDDIHAVDYVARGASSHYKPHDIHHSSSSHISSAHAQSKSTFTIPCHHIRIGDLVILQHRPCQIIRITTSAQTGQHRYLGVDLFTKQLHEEPCVVSHPSPSVVLHSLLGPVFKQYRLIDIRDDGKLVAMTETGEIKQGLKVVDQGHLWGKLKEAFGHGAGSVRVLVISDHGKELAVDYKIVHLAHKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5G488</accession>
    <name>D5G488_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00004001001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835210.1">
        <property type="nucleotide sequence ID" value="XM_002835164.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G488"/>
    <dbReference type="STRING" id="656061.D5G488"/>
    <dbReference type="GeneID" id="9184956"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004001001"/>
    <dbReference type="eggNOG" id="KOG2134">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014938_3_1_1"/>
    <dbReference type="InParanoid" id="D5G488"/>
    <dbReference type="OMA" id="AADWKWW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046404">
        <property type="term" value="F:ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046403">
        <property type="term" value="F:polynucleotide 3'-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000012">
        <property type="term" value="P:single strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01625">
        <property type="entry name" value="HAD_PNP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000737">
        <property type="entry name" value="Bifunctional polynucleotide phosphatase/kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006549">
        <property type="entry name" value="HAD-SF_hydro_IIIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013954">
        <property type="entry name" value="PNK3P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006551">
        <property type="entry name" value="Polynucleotide_phosphatase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01664">
        <property type="entry name" value="DNA-3'-Pase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01662">
        <property type="entry name" value="HAD-SF-IIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12083">
        <property type="entry name" value="BIFUNCTIONAL POLYNUCLEOTIDE PHOSPHATASE/KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12083:SF9">
        <property type="entry name" value="BIFUNCTIONAL POLYNUCLEOTIDE PHOSPHATASE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13671">
        <property type="entry name" value="AAA_33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08645">
        <property type="entry name" value="PNK3P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="487" mass="55077" checksum="CCE01BDDE26556DB" modified="2010-06-15" version="1">MRLRTNASTILSPRLSTSHLLHQHHHLPLNPNSYRRHLGNRHHRVFTSFFSSTYISSMPVGSPKRRRSADGSAPPAKRKVQSTTTQATVVNFFKPASKKEPEHTIWKVVDDTLLVGTYFNRASPNKTKPAASDVKRRVAGFDLDSTLITTESGNVFAKTATDWKWWSPCVPDTLRKLHDDGYIIVIFTNQGFLKQPKKDSSNLTKFKLKLAAVLDSLDIPVTVYAATANDRYRKPRVGMWEEMVDDYDLDAHGVDLDASYLVGDAAGRDGDHSDSDRHWAANVGIGFRTPEEFFLGKMPKSMAHRFDPLKYIKSEPQALVFTKSSKQEIVLFVGPPGAGKSTFYRRNLEPLGFERVNQDTLKTKDRCLKVSDSLLEGGKSVTVDNTNPDVTTRAAWISLAKKHKVPIRCVHFIAPTDLCQHNDAVRALGGELVNPEKRKILPKIAFTNFASRFIKPRIDEGFQDMTEIEFQWEGGDPELQIWRKYWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4K9</accession>
    <name>D5G4K9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cholesterol oxidase</fullName>
            <ecNumber evidence="13">1.1.3.6</ecNumber>
            <ecNumber evidence="11">5.3.3.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Cholesterol isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">GSTUM_00004203001</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="12">Steroid metabolism; cholesterol degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GMC oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.3.6" evidence="13"/>
    <dbReference type="EC" id="5.3.3.1" evidence="11"/>
    <dbReference type="EMBL" id="FN429988">
        <property type="protein sequence ID" value="CAZ79452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835295.1">
        <property type="nucleotide sequence ID" value="XM_002835249.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4K9"/>
    <dbReference type="STRING" id="656061.D5G4K9"/>
    <dbReference type="GeneID" id="9188388"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004203001"/>
    <dbReference type="eggNOG" id="ENOG502QSPJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002483_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4K9"/>
    <dbReference type="OMA" id="GHLDAWM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016995">
        <property type="term" value="F:cholesterol oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004769">
        <property type="term" value="F:steroid Delta-isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052542">
        <property type="entry name" value="Cholesterol_Oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000172">
        <property type="entry name" value="GMC_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007867">
        <property type="entry name" value="GMC_OxRtase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47470">
        <property type="entry name" value="CHOLESTEROL OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47470:SF1">
        <property type="entry name" value="FAD-DEPENDENT OXIDOREDUCTASE 2 FAD BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05199">
        <property type="entry name" value="GMC_oxred_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00732">
        <property type="entry name" value="GMC_oxred_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="8" id="KW-1207">Sterol metabolism</keyword>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="17">
        <location>
            <begin position="95"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase C-terminal" evidence="18">
        <location>
            <begin position="566"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF05199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAZ79452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1267" mass="137069" checksum="7B3154BF35D331DE" modified="2010-06-15" version="1">MSQSPERTPAVPNGPQHPPPAASQQTVSLPHEILQADPATFVKGGIPLTENDYAQSSADTGAAEDAKFPRISRPVPMMRVEYDVVVVGSGYGGAVTASRMARAGKSVCVLELGRERWPGEYPVGLLEAAPEVHVSGNRGKNGEPVVDATIGDPTGLYHLVLGEGQNAFVANGLGGTSLINANIFLSADKRTLQLPAWPQEISSNPASLDTYYTRAASVLQPVPYPDDFPQLKKLSVLEKQAELMGQREHFYRAPQTTAFTNGPNSSGIEMRASTLSGQDCTGVNDGSKGSVLMNYLPDAWNWGAELFCECEVRYVKRDPSNNAWLVLFAWHGDKRAKFEDFHRELMWVKAREFVFLGAGTLGTTEILLRSQDHGLKTSSEIGRGMSGNGDILSFGYNTDFEVNSIGREDPGNHPPIGPTITGVIDMRDAMPDVLDGYVLEEGAAPEALSKLLQGMLELMPGKVYPENYGLSEKLRHLFSRAEARLLGPYTPGGSVNRTQIYLIMSHDSNEATLTLENGKPYLRFLGVGRSAHVKELNEMLAKATSKIGGTLINSPFYSLLGEEEITVHPIGGANMSSDGTGRNGATNHFGEVFTGTGDDVHPGLVVVDGSMIPCALGVNPFATITALAERSVEGVAAKRQIRIDYATRNTRLNLFAPPMRSFPLTRDMAEAQQAISSAAGKSGIRFTEVMEGYMYIGDDIEDFQVAADAARGSSTSARFLLSVDSYSTQNLIERSDHAALATGTFACGALSRDPFMVLRGEVQFFSLDSDVADGKNLAYKLDLVSSDGKLLHFNGYKVIDSKMAFPASNTWKATTTLYVTLTRPDKTVVARGVLTIPWRNFVSEISSFGQTGSTVSGRIGATVDFLGYFASQTAGYFFSPLRPLQYPTLTTSGYYRKQPPTEIVTLTAQDGVQTFMHVYTPDPTSGPRQNNQNGGGGGLPPILMIPGAAVDHRIYALPTIPTNAVDYFLARGHTIYIPTHRVGRTPLAKSSQTTFSACLDVKAALEHIRRETPSQVYVIAHCAGSIALGCGLLDGTIPASWLKGLTASQVFISPRFAKANMIKARLPLPLPRVYSKIVGEYFDCATSPTDALPQRLMNQLLRFYPVGSRAEICNSAVCHRSELVFGRLWSHSGLNGATHANLGNFLGGTSMRCLTHLMRMGSSGYVMDDEFASLITEIGLRRLEGLKVFFVQGGENAVYDPENTQTSYELLREEFGSAGVGRVIFENRGHLDCWMGKSAYMDVYPAVANHIDEVMSGGVTGEVAGKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>D5G547</accession>
    <name>D5G547_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">DASH complex subunit SPC34</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Outer kinetochore protein SPC34</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">GSTUM_00000252001</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
            <location evidence="3">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DASH complex SPC34 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429996">
        <property type="protein sequence ID" value="CAZ79632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835475.1">
        <property type="nucleotide sequence ID" value="XM_002835429.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G547"/>
    <dbReference type="STRING" id="656061.D5G547"/>
    <dbReference type="GeneID" id="9186330"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000252001"/>
    <dbReference type="eggNOG" id="ENOG502SCS2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064328_0_0_1"/>
    <dbReference type="InParanoid" id="D5G547"/>
    <dbReference type="OMA" id="TRRGTMF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042729">
        <property type="term" value="C:DASH complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008608">
        <property type="term" value="P:attachment of spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013966">
        <property type="entry name" value="Spc34"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08657">
        <property type="entry name" value="DASH_Spc34"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="16" id="KW-0137">Centromere</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="10" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="12 19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="138"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00044112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00044346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CAZ79632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="225" mass="24986" checksum="73EED015DF0F0CDD" modified="2010-06-15" version="1">MSTLLSPHLQQISAAANSLSSLPFAPPKVFTNALLRSTDIVSLIRDTDAHERALFTVPAAPTEKDMKKSRMSIAPRRNTAVYSALGGDMVEKLRRGGAGGVGGGVGGVATGEVDVEVLLMGAERLLGVYHIPGAEERIHRARERYDHLTESLENYEALVEAQRQQLDLLQGPEPEVVVVEEEEEAGEEITDEMLAAEEEEIRELEERKAELEERIRNVDRRMYRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>D5G586</accession>
    <name>D5G586_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00004217001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835522.1">
        <property type="nucleotide sequence ID" value="XM_002835476.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G586"/>
    <dbReference type="STRING" id="656061.D5G586"/>
    <dbReference type="GeneID" id="9183795"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004217001"/>
    <dbReference type="eggNOG" id="ENOG502QYNS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007604_0_0_1"/>
    <dbReference type="InParanoid" id="D5G586"/>
    <dbReference type="OMA" id="CIHSVLP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050987">
        <property type="entry name" value="AtrR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910:SF1">
        <property type="entry name" value="MISCELLANEOUS ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910">
        <property type="entry name" value="TRANSCRIPTION FACTOR PDR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="5">
        <location>
            <begin position="108"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="180"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="672"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="739"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="146"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="42"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="672"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="748"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="795"/>
            <end position="806"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ79679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="806" mass="89305" checksum="50D0489D8F41F967" modified="2010-06-15" version="1">MEDRASSIQHPPFQGTLPPPNGMHSYDPGMNTPTSAPLPGTASAQSQHMGINNSNHPSFQPYNSNSVSPGPSRDGQMPSPSAGTPMHPGQVMTGTQKRAYRQRRKDPSCDACRERKVKCDATDVASCSECASRNVKCQFTKETNRRMSSIKQVQDLEKQLLAAKEQIRKLMNGQQAKENMLESGGADSNAPDIPITVPDLNSNPQRRPRPPVTQDLTDVRAHLRNYSRGVFKPPPPYRQVGLQSNFSPPLPELPPQSVADAILAQYYQTIHSVIPILHWPQFVQRYEEVRKQGDLSGVPPYWGSVLFAVFACGLLYTVDPQIKASYPDHGRSFIGYSRQLTDLFNDEFTIDHARSALLTSIFLTELNCKSAAWTWLGSTVRIAQDIGLHRESGPWPVVEGEMRRRVWWGIYVWDRLLSVELGRVLLIEDQDCDISLPCALDDHFIYDEGLLVPNGSSQQPANFLLTTIHVVRLIGPLLKNLQVPVISPQNLACFDTHFTSCLSAFPANCHINHPNPLEPRHLSPIFHLQNCRLVLHRHNLSTLCPFEARAAALDSCVSAAKDTVHFLSRAMQYGYGTGWQNMMATCASTMICTHIWRCSLFLCFRGFYNEALVCVQVSNAIGEIRDVNVACGRNLYGFLRLLMEKLDAGVSLAHDEGMMALVSGDVQGSTESSWVWNGSETGQALNHGSPGHETPVANGKDYPHTNGHAEPPASLSREEAVDWGGWSQLERMLQELAHDKAAREQAHQQRQQQHQQQQQQQQPPPPPPPHQQHQLAPLPWNGGVGGSGRSAPAPTASSRISIANII</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5N9</accession>
    <name>D5G5N9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00001507001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430000">
        <property type="protein sequence ID" value="CAZ79832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835675.1">
        <property type="nucleotide sequence ID" value="XM_002835629.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5N9"/>
    <dbReference type="STRING" id="656061.D5G5N9"/>
    <dbReference type="GeneID" id="9187509"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001507001"/>
    <dbReference type="HOGENOM" id="CLU_1338395_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5N9"/>
    <dbReference type="OMA" id="DRYGYNN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ79832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="205" mass="22714" checksum="1381E3BA264FA352" modified="2010-06-15" version="1">MCMIKVRPEIEEEVVVPARPVGHHRRTGSRDSRIQETTVVETIKAPPPPPSTLSLSNNPGTTTIIQAPEPPASIIGSAADSEMVRHHHYPYDSPRASVVSARSRDYYVDSSAYAARSPRSSYRYIDARGSGRVDGVGGVGGSGRKEMDYVRGDRYGYNNYHGRRSGSMNYVNPRHSTASHRSARSMREKVVVVDEYGREKFYGTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5Q6</accession>
    <name>D5G5Q6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00001434001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the OSBP family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430001">
        <property type="protein sequence ID" value="CAZ79849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835692.1">
        <property type="nucleotide sequence ID" value="XM_002835646.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5Q6"/>
    <dbReference type="FunCoup" id="D5G5Q6">
        <property type="interactions" value="94"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G5Q6"/>
    <dbReference type="GeneID" id="9187527"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001434001"/>
    <dbReference type="eggNOG" id="KOG2210">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012334_0_2_1"/>
    <dbReference type="InParanoid" id="D5G5Q6"/>
    <dbReference type="OMA" id="SSYWTEH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008142">
        <property type="term" value="F:oxysterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120009">
        <property type="term" value="P:intermembrane lipid transfer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.2720:FF:000003">
        <property type="entry name" value="Oxysterol binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.120:FF:000010">
        <property type="entry name" value="Oxysterol-binding protein homolog 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2720">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037239">
        <property type="entry name" value="OSBP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018494">
        <property type="entry name" value="Oxysterol-bd_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF184">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN HOMOLOG 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144000">
        <property type="entry name" value="Oxysterol-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01013">
        <property type="entry name" value="OSBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ79849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="407" mass="45572" checksum="54FAD8823AE0C4B6" modified="2010-06-15" version="1">MSSSKEEGGAVPAAAKSSWGPFLKSIASFTGDLASLTAPPFILSSTSLTEYSGYWAEHPEIFVAPAQEKDPQKRALLVLKWFLTTLKQQYSSRNEKLGSEKKPLNPFLGELFLGKWEDPTGKIGETQLISEQVSHHPPVTAYAILNQKQGVKLEGYNGQKASFSRGTISVRQVGHAKYHLKKFDEDYLITLPSLHIEGIVYGSPYVELNRSSTIHSSTGYVAQIDYSGKGWVTGKKNTFSATLRKEGSKDILYTIDGQWSDGFVIKEGKTKELETYNAKTTEITPLYIAPNKEQDPLESRRAWAKVQESINTGDLETTGIEKSKIENEQRELRKKEKEEGREWERRYFTRVMDDPVFTRLAESPGIAAEEGKTDGIWIFDEKKFAQVAEKTRVVEEAKAEEVVTTAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5G605</accession>
    <name>D5G605_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="16">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">GSTUM_00001710001</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 2 H(+) = thiamine phosphate + CO2 + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:47848"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
            <dbReference type="ChEBI" id="CHEBI:62890"/>
            <dbReference type="EC" id="2.5.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 2 H(+) = thiamine phosphate + CO2 + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:47844"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
            <dbReference type="ChEBI" id="CHEBI:62899"/>
            <dbReference type="EC" id="2.5.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>4-methyl-5-(2-phosphooxyethyl)-thiazole + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + H(+) = thiamine phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22328"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
            <dbReference type="ChEBI" id="CHEBI:58296"/>
            <dbReference type="EC" id="2.5.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-(2-hydroxyethyl)-4-methylthiazole + ATP = 4-methyl-5-(2-phosphooxyethyl)-thiazole + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24212"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17957"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58296"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.50"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine phosphate from 4-amino-2-methyl-5-diphosphomethylpyrimidine and 4-methyl-5-(2-phosphoethyl)-thiazole: step 1/1.</text>
    </comment>
    <dbReference type="EMBL" id="FN430005">
        <property type="protein sequence ID" value="CAZ79948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835791.1">
        <property type="nucleotide sequence ID" value="XM_002835745.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G605"/>
    <dbReference type="FunCoup" id="D5G605">
        <property type="interactions" value="112"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G605"/>
    <dbReference type="GeneID" id="9187626"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001710001"/>
    <dbReference type="eggNOG" id="ENOG502QS2M">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019943_1_1_1"/>
    <dbReference type="InParanoid" id="D5G605"/>
    <dbReference type="OMA" id="GQTDMPI"/>
    <dbReference type="UniPathway" id="UPA00060">
        <property type="reaction ID" value="UER00139"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004417">
        <property type="term" value="F:hydroxyethylthiazole kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004789">
        <property type="term" value="F:thiamine-phosphate diphosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01170">
        <property type="entry name" value="THZ_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00564">
        <property type="entry name" value="TMP_TenI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00228">
        <property type="entry name" value="Thz_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00097">
        <property type="entry name" value="TMP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000417">
        <property type="entry name" value="Hyethyz_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036206">
        <property type="entry name" value="ThiamineP_synth_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022998">
        <property type="entry name" value="ThiamineP_synth_TenI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034291">
        <property type="entry name" value="TMP_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006830">
        <property type="entry name" value="PRK09355.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00693">
        <property type="entry name" value="thiE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20857:SF23">
        <property type="entry name" value="THIAMINE BIOSYNTHETIC BIFUNCTIONAL ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20857">
        <property type="entry name" value="THIAMINE-PHOSPHATE PYROPHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02110">
        <property type="entry name" value="HK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02581">
        <property type="entry name" value="TMP-TENI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01099">
        <property type="entry name" value="HYETHTZKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51391">
        <property type="entry name" value="Thiamin phosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiamine phosphate synthase/TenI" evidence="15">
        <location>
            <begin position="10"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAZ79948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="540" mass="57496" checksum="F9693E232125F979" modified="2010-06-15" version="1">MPPPRPNLELYLVTDSVNLPFNSTVYSQVEACLSAKHPPTIVQLREKHLSTAKFIELARDIYALTSKNGVPLLINDRVDVALAVGCEGVHLGWDDIDVPTARKLLGPEKIIGLSVSNHEQLEKALGLDPTYLGIGPVFSTPTKPDHNPPLGTSGVRSLLSAIPITMQAVAIGGINHGNVQRVLFQSKDISGRRLDGVAVVSAIMASPLPVGACNLLHETVNWPPLFVPTDYSLRTEDFPIYRDNIHKHKPLVHHITNNVVKSISANISLAIGASPIMSENPAEFADLAALPNVGCVLNMGTFASSKESQDLFLGALREHNVRGNPVIFDPVGAGATNARREFAQMIVGAGYCTVIKGNEGEIRSLAGLETAKMRGVDGAGEGGSEEELAKVVHDLASLENNIVLMTGPKDYISNGARTFCLTNGSAYQSRVTGVGCALGSVIAAFCAAAPVDESLIGPVISAIGMYNIAAERAEKLMESGDQPGKWAAAFVDCLAVMAMSDENLTELISVHEVKELANLEKWGRPTGREGLKQGEDLTEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6E4</accession>
    <name>D5G6E4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Topoisomerase 1-associated factor 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00004446001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the timeless family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430009">
        <property type="protein sequence ID" value="CAZ80087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835930.1">
        <property type="nucleotide sequence ID" value="XM_002835884.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6E4"/>
    <dbReference type="FunCoup" id="D5G6E4">
        <property type="interactions" value="45"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G6E4"/>
    <dbReference type="GeneID" id="9181172"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004446001"/>
    <dbReference type="eggNOG" id="KOG1974">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004390_0_0_1"/>
    <dbReference type="InParanoid" id="D5G6E4"/>
    <dbReference type="OMA" id="VNHHRHT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031298">
        <property type="term" value="C:replication fork protection complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000076">
        <property type="term" value="P:DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043111">
        <property type="term" value="P:replication fork arrest"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044998">
        <property type="entry name" value="Timeless"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006906">
        <property type="entry name" value="Timeless_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22940:SF4">
        <property type="entry name" value="PROTEIN TIMELESS HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22940">
        <property type="entry name" value="TIMEOUT/TIMELESS-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04821">
        <property type="entry name" value="TIMELESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0236">DNA replication inhibitor</keyword>
    <keyword evidence="6" id="KW-0469">Meiosis</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Timeless N-terminal" evidence="9">
        <location>
            <begin position="34"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="553"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="792"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="895"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1024"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="553"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="569"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="928"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1064"/>
            <end position="1083"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ80087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1117" mass="126803" checksum="C510387D6B401CC9" modified="2010-06-15" version="1">MSSEGEGDMVLSRLPETVDPMILGGTSMVEDGGYSLGDDALACLKDLKRWLKLYDQKMNRFDVARCMAEANLVKGDLLEILSGWEKDEIENRIKQRLALGCVELLVPLTWPFERYNSQTTVNHHRHIPVLQFAQAGYKKAILQHPSKRILSNIVRVALPSIAVPPNERTERDEGIIKLVLYFFRNLVMIEHPMITETDTGEEISRSATIEALKEQNVLHLLLAMSSEMGNEFNTQDVVVMEVIYHLVKSVDIEKMFLTDEQESQKQGQDLTALLKLEDEMKRASIRGASTRHNRFGTTVWIERGDKTRSFVSGQGALLGKSTGLDKMDASKKWKKPGGADTKGISRKTEHDMSLTLERRAKAKLREFVEGFLDAGFNPLFSHLRRAIDRDAQRLVESNQQQYFYLIAWFLKAERTRRKTFANSEDPETSDIESFSLVASVLNQESLITLNRRMTDWFDTKQWTPLQSAMRCFTQILLTVQDMAVSPLEDDQDIAENIQNRLFYEEVTLDLVVMILRAYTRQPFGWLDDCTEMVHVHLRMLERFSKQHEHMFIRSRKRARAQRKKNPTSEDGDGGVPEEGDEEMAELSKATMKERAFDYSRFEQKFLTQSCVNTFLAFIGNYKELNYSQMKRAIVFFHRVFVKREQEVLLFRIDIVELFNRILQGPDGLPSSHSARKEVDQFFKHYMRKLVKMLEKRPELYVELLFTKINSTMHYLQFGYEKQINVSKPREAAELRIKPGLEFNEQVSVAVGALLDENKGDAVDWLKTVLAAAISERKSWEAGEEARLALGAEAADPDSETNPQAPPSPPPFINITPDNEVRQTALLKDGKLRLLLTTLKFEKIDAVDTLNTRWIIPSQLTSIDLQTSLELLKLNTDNPPIFPDGHLAEDFISRKPKPRVPAFDSDSGSDLEPLFEPGGPTPMPGAEKPKKKSARKRRRKDPMESTELGSDLDDAAIERALARRQKEKERMVMIKSSLFVHDSDDESDEERDKLFYEKERMQAAMGPVGLDGSAGPQMDFMEYGFGGVGEDWAGSTGAGEGNGRKRKNVAGGQAKAKKRRAIESGSDDDNGSSADEESSDDGDGEERPFAKPPTMPDVRTPPARPRRRGPVIVSSDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6L4</accession>
    <name>D5G6L4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00002094001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430011">
        <property type="protein sequence ID" value="CAZ80157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836000.1">
        <property type="nucleotide sequence ID" value="XM_002835954.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6L4"/>
    <dbReference type="GeneID" id="9187183"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002094001"/>
    <dbReference type="HOGENOM" id="CLU_2943496_0_0_1"/>
    <dbReference type="InParanoid" id="D5G6L4"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ80157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="60" mass="6553" checksum="5EA38BC0EF5E81CB" modified="2010-06-15" version="1">MPVLLSGSYVASQIPTRVSYLTRSPQFRFPPTSGGRPWSLQPGNCGDRLRVRMTVKSASG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6L5</accession>
    <name>D5G6L5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Fatty acid synthase subunit alpha</fullName>
            <ecNumber evidence="3">1.1.1.100</ecNumber>
            <ecNumber evidence="4">2.3.1.41</ecNumber>
            <ecNumber evidence="2">2.3.1.86</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="30" type="ORF">GSTUM_00002095001</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="30 31" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="30 31">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>a (3R)-hydroxyacyl-[ACP] + NADP(+) = a 3-oxoacyl-[ACP] + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17397"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:9945"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:78827"/>
            <dbReference type="EC" id="1.1.1.100"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>a fatty acyl-[ACP] + malonyl-[ACP] + H(+) = a 3-oxoacyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:22836"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:14125"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:138651"/>
            <dbReference type="EC" id="2.3.1.41"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>acetyl-CoA + n malonyl-CoA + 2n NADPH + 4n H(+) = a long-chain-acyl-CoA + n CoA + n CO2 + 2n NADP(+).</text>
            <dbReference type="EC" id="2.3.1.86"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 22">Belongs to the thiolase-like superfamily. Fungal fatty acid synthetase subunit alpha family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.100" evidence="3"/>
    <dbReference type="EC" id="2.3.1.41" evidence="4"/>
    <dbReference type="EC" id="2.3.1.86" evidence="2"/>
    <dbReference type="EMBL" id="FN430011">
        <property type="protein sequence ID" value="CAZ80158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836001.1">
        <property type="nucleotide sequence ID" value="XM_002835955.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5G6L5">
        <property type="interactions" value="309"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G6L5"/>
    <dbReference type="GeneID" id="9187184"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002095001"/>
    <dbReference type="eggNOG" id="ENOG502QQJX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000114_0_0_1"/>
    <dbReference type="InParanoid" id="D5G6L5"/>
    <dbReference type="OMA" id="FPTLPDW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005835">
        <property type="term" value="C:fatty acid synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004316">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004312">
        <property type="term" value="F:fatty acid synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004321">
        <property type="term" value="F:fatty-acyl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008897">
        <property type="term" value="F:holo-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0101026">
        <property type="term" value="P:mitotic nuclear membrane biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900535">
        <property type="term" value="P:palmitic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00828">
        <property type="entry name" value="elong_cond_enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08950">
        <property type="entry name" value="KR_fFAS_SDR_c_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.470.20:FF:000005">
        <property type="entry name" value="Fatty acid synthase alpha subunit FasA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2490:FF:000001">
        <property type="entry name" value="Fatty acid synthase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000168">
        <property type="entry name" value="Fatty acid synthase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.20">
        <property type="entry name" value="4'-phosphopantetheinyl transferase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00101">
        <property type="entry name" value="AcpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008278">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037143">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002582">
        <property type="entry name" value="ACPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040899">
        <property type="entry name" value="Fas_alpha_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047224">
        <property type="entry name" value="FAS_alpha_su_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026025">
        <property type="entry name" value="FAS_alpha_yeast"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041550">
        <property type="entry name" value="FASI_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050830">
        <property type="entry name" value="Fungal_FAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004568">
        <property type="entry name" value="Ppantetheine-prot_Trfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00556">
        <property type="entry name" value="pantethn_trn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10982:SF21">
        <property type="entry name" value="FATTY ACID SYNTHASE SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10982">
        <property type="entry name" value="MALONYL COA-ACYL CARRIER PROTEIN TRANSACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01648">
        <property type="entry name" value="ACPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18325">
        <property type="entry name" value="Fas_alpha_ACP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18314">
        <property type="entry name" value="FAS_I_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000454">
        <property type="entry name" value="FAS_yeast_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56214">
        <property type="entry name" value="4'-phosphopantetheinyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="KS3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52004">
        <property type="entry name" value="KS3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="7" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 25" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 25" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="15" id="KW-0520">NAD</keyword>
    <keyword evidence="13" id="KW-0521">NADP</keyword>
    <keyword evidence="14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6 22 26" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 22" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carrier" evidence="28">
        <location>
            <begin position="83"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Ketosynthase family 3 (KS3)" evidence="29">
        <location>
            <begin position="939"/>
            <end position="1476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="37"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="1170"/>
            <end position="1192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="27">
        <location>
            <begin position="49"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="27">
        <location>
            <begin position="1180"/>
            <end position="1191"/>
        </location>
    </feature>
    <feature type="active site" description="For beta-ketoacyl synthase activity" evidence="23">
        <location>
            <position position="1122"/>
        </location>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <begin position="1589"/>
            <end position="1591"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="1589"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="1590"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="1591"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="1615"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="1625"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <begin position="1634"/>
            <end position="1650"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <begin position="1658"/>
            <end position="1661"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <begin position="1688"/>
            <end position="1690"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="1689"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="1690"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="O-(pantetheine 4'-phosphoryl)serine" evidence="26">
        <location>
            <position position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00049541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRNR" id="PIRNR000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR000454-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR000454-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PIRSR" id="PIRSR000454-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PIRSR" id="PIRSR000454-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS52004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="EMBL" id="CAZ80158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1703" mass="187887" checksum="B44FF524DA910B06" modified="2010-06-15" version="1">MARRTLAAKYEAYDAAQSLQRQILCYNKDAKEIYYDVEPVEEEEEKPTEASSAPSVSPAPAAGASPVAVAPPASTGPAVAVADEPVQAVDIVRSLIAQKLKKQLSEIPLSKAIKDLVNGKSTLQNEILGDLQKEFGNTPEKPEDTPLDELGVAMQATFNGQLGKQSSSMVARMISSKMPGGFNITTARKYLTDRYGLQSGRQDSALMLAITMEPPARLGSEGDAKAFLDDVVTKYAASAGISLSGPAAGGAAAGASGGMMMDPAAIDALTKDQRALFKQQLELLARYLKMDLRSGEKAFVGSQEANKLLQAQIDLWNVEHGDFYAFGIEPSFSPLKVRVYDSSWNWARQDALNMYFDIIFGRLRLVDREIVSQCIRIMNRSNPTLVEFMQYHIDNCPAERGETYRLAKDLGYQLIANCQEVLGTAPVYKDVQYPTAPQTTIDAKGNMKYSEVPRPAVRKLEHYVKEMAAGGKLTEYVYLDCLEQAAQSGVTFENRNVLMTGAGAGSIGADVLQGLISGGARVVVTTSRFSREVTEYYQAMYSKFGSRGSQLIVVPFNQGSKQDVEALVNYIYDEKNGLGWDLDYVVPFAAISENGREIDGIDSKSELAHRIMLTNLLRLLGMVKTNKASRGFETRPAQVILPLSPNHGTFGNDGLYSESKSALEALFQRWHSESWGSYLTICGAIIGWTRGTGLMGANNSIAEGVEKLGVRTFSQQEMAFNLLGLMAPAIVDICQIEPVFADLNGGFQYIPNLKDVCAQLRQNLLETCEVRKVVTAETSIEHKIVNGEVSEKLYQKVTIQPRANMKFEFPELPDYKKEVEPLAQNLRGMVDLEKVVVITGFAEVGPWGNSRTRWEMEAYGKFSLEGCIEMAWIMGLIRHFKGPIKGNQYTGWVDVKTGEPVEDKDIKPKYERYILEHSGIRLIESDLFNGYDPKKKELLQEVVIEHDLEQFEASKETAEEFKRHHGDKVEIFEIADSGEYTVRVKKGAILFIPKALKFDRLVAGQIPTGWDAKKYGIPDDIIDQVDPVTLYLLVCVVESLLAAGITDPYEFYKYVHLSEVGNCVGSGVGGMTALRGMYKDRHMDRPIQKDVLQESFINTMSAWVNMLLLSSTGPIKTPVGACATAVESLDIGYETIVEGKARVCFVGGFDDFQEEGSYEFANMKATSNAEEELLHGRTPQEMSRPTTTTRSGFMESQGCGVQLIMTAKLALEMGVPIYGIVALTATATDKIGRSIPAPGQGILTTARETSSRFPSPLLNIKYRRRQLELRKSQIKQWTESELLYLQEEVAAMKAEKVDFDENEYMQDRAGHIERESQRQEREALNAWGNDFWKQDPRIAPLRGALAVWGLTIDDLGVASFHGTSTKANDKNESDAINKMMMHLGRHKGNAVLGIFQKYLTGHPKGAAGAWMMNGAIQVLNSGLVPGNRNADNVDKSLEQFDYIVYPSRSIQTDGVKAFSVTSFGFGQKGAQAIGVHPKYLFAILDEQTYETYRAKVNSRYKKAYRYYHTSLVENNLFQAKNDPPYSPDQESQVLLDPLARVQADRKTNSYTYPAQIQKKRPDPSVENTRKLVEHLASATAGENSKIGVDVELISSINIDNENFIERNFTPAEQEYCRKAPSPQASFAGRWSAKESVFKALGVSSKGAGAPLKDIEILSDDKGAPIVVLHGDAKEAAEKAGVKGVQVSISHSDAQAIAIAVSSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6S0</accession>
    <name>D5G6S0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00002241001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430014">
        <property type="protein sequence ID" value="CAZ80213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6S0"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002241001"/>
    <dbReference type="HOGENOM" id="CLU_3052079_0_0_1"/>
    <dbReference type="InParanoid" id="D5G6S0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ80213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="54" mass="6375" checksum="0AA2FC2E7B975B2D" modified="2010-06-15" version="1">MCFFYLRGFRRMFFKLFPPALVWNSLKDGHLWQAGDGGRISCISHVFLLLRVII</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6S2</accession>
    <name>D5G6S2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00002243001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the syntaxin family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430014">
        <property type="protein sequence ID" value="CAZ80215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836058.1">
        <property type="nucleotide sequence ID" value="XM_002836012.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6S2"/>
    <dbReference type="STRING" id="656061.D5G6S2"/>
    <dbReference type="GeneID" id="9187240"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002243001"/>
    <dbReference type="eggNOG" id="KOG0810">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042423_0_1_1"/>
    <dbReference type="InParanoid" id="D5G6S2"/>
    <dbReference type="OMA" id="WIAICCA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15849">
        <property type="entry name" value="SNARE_Sso1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006011">
        <property type="entry name" value="Syntaxin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF307">
        <property type="entry name" value="PROTEIN SSO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00804">
        <property type="entry name" value="Syntaxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00503">
        <property type="entry name" value="SynN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="272"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="8">
        <location>
            <begin position="198"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ80215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="316" mass="35067" checksum="480250BB81CD520E" modified="2010-06-15" version="1">MSYQGYEMESPLLQNPEPHGQAPGGQGYGGHQALPLNSFLNEVTGIRGLLTTFRQAVLDIGALHNQTLQSYDNGATSGSTQQLESLVAETSVLTKQLRDSIKSLERDALIMDEYSTQNTKRQQIDKLKGDFDKELRAYQQTENSFKTRYRDQMARQYRIVNPDATEADVERMVESGETQVFSQALLSSGRSREANSVNAAVRARHAEIQRIETTLIELLGLFEQMAEQVVLDEPKVMQVEENTTKVERDMEQATAHMGKAVESARSARKKKWICLGIGVGIVVVIAIIITVVVVVRNSGKNNGNKDNNQDPKPTAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>D5G752</accession>
    <name>D5G752_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00002347001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BLOC1S2 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430021">
        <property type="protein sequence ID" value="CAZ80345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836154.1">
        <property type="nucleotide sequence ID" value="XM_002836108.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G752"/>
    <dbReference type="STRING" id="656061.D5G752"/>
    <dbReference type="GeneID" id="9184279"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002347001"/>
    <dbReference type="eggNOG" id="ENOG502SG83">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_157384_0_0_1"/>
    <dbReference type="InParanoid" id="D5G752"/>
    <dbReference type="OMA" id="QSVYQNP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019269">
        <property type="entry name" value="BLOC1_su2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47882">
        <property type="entry name" value="BIOGENESIS OF LYSOSOME-RELATED ORGANELLES COMPLEX 1 SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47882:SF1">
        <property type="entry name" value="BIOGENESIS OF LYSOSOME-RELATED ORGANELLES COMPLEX 1 SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10046">
        <property type="entry name" value="BLOC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="63"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ80345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="112" mass="12660" checksum="9DB4B6A47149557A" modified="2010-06-15" version="1">MAAQKTANNVNDVLATLENVIRADTEATVGDLDLLNRVNLHATERYKAIADAAVGLETDSEYLGQKYAEIEKYKQQVDNLDERVGDLEAIVKELEEWTGELEIKVRRLSRRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>D5G780</accession>
    <name>D5G780_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00002506001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430022">
        <property type="protein sequence ID" value="CAZ80373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836182.1">
        <property type="nucleotide sequence ID" value="XM_002836136.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G780"/>
    <dbReference type="STRING" id="656061.D5G780"/>
    <dbReference type="GeneID" id="9185271"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002506001"/>
    <dbReference type="HOGENOM" id="CLU_934438_0_0_1"/>
    <dbReference type="InParanoid" id="D5G780"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070860">
        <property type="term" value="C:RNA polymerase I core factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001164">
        <property type="term" value="F:RNA polymerase I core promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001181">
        <property type="term" value="F:RNA polymerase I general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042790">
        <property type="term" value="P:nucleolar large rRNA transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053029">
        <property type="entry name" value="RNA_pol_I-specific_init_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007224">
        <property type="entry name" value="TIF_Rrn11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28244">
        <property type="entry name" value="RNA POLYMERASE I-SPECIFIC TRANSCRIPTION INITIATION FACTOR RRN11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28244:SF1">
        <property type="entry name" value="RNA POLYMERASE I-SPECIFIC TRANSCRIPTION INITIATION FACTOR RRN11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04090">
        <property type="entry name" value="Rrn11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="275"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="278"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="298" mass="33547" checksum="DD826B843CFE4091" modified="2010-06-15" version="1">MNISTDQRSYFALPTYPRNPSPPKRPLSASPPGTPSPPRTPPVVPAWTPINEPPSLPSSPSPSPKPSDNDTEVEHLSPKAIHRRGNHSRRFQLLQQSCTEPCIDLRPLWSIGLDLLLRIPTTTAPTNANTEKAIEYLNRLILFYPYRPRIHAHHPAIISAARRKEGDIGRTGPASALEFYPALFRLRVMQPEGLEKVREEIEELMLTPPWPDMVGLWLMKGMVLNALAEEERDLGRKEQLRTGAEECFDEVREKGGKVPEEAVWEEGMDNVEVVEGFSSDEDEILDGDDYDERMDDSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7M6</accession>
    <name>D5G7M6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00004636001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430031">
        <property type="protein sequence ID" value="CAZ80519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836328.1">
        <property type="nucleotide sequence ID" value="XM_002836282.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7M6"/>
    <dbReference type="STRING" id="656061.D5G7M6"/>
    <dbReference type="GeneID" id="9186998"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004636001"/>
    <dbReference type="eggNOG" id="KOG3043">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054590_0_1_1"/>
    <dbReference type="InParanoid" id="D5G7M6"/>
    <dbReference type="OMA" id="SKACCTR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002925">
        <property type="entry name" value="Dienelactn_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47668:SF1">
        <property type="entry name" value="DIENELACTONE HYDROLASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47668">
        <property type="entry name" value="DIENELACTONE HYDROLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_6G01940)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01738">
        <property type="entry name" value="DLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dienelactone hydrolase" evidence="1">
        <location>
            <begin position="29"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="251" mass="27194" checksum="0B22AAE9E458E186" modified="2010-06-15" version="1">MASKACCEIPPVVTHDYTPNGTFETTANFKSYVTGPKSSKTGILVIFDIFGYFPQTLQGADILASAGHLVVMPDFFKGQAADPDCYPPSTPEKLKALMGFIHGPAKLEDGEANVRAVAEGLKGEFAGVERWGVVGYCWGGKVTARVSGPGTPFTASAQLHPSFMSVEDLRKVTIPQLVLPSKDEPADMVEAYKKELAVHPDGAVKTKSYIEVYDKAIHGWMAARANLADEDARNEYERGYKQVVTFLGDNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7N1</accession>
    <name>D5G7N1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00004642001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430031">
        <property type="protein sequence ID" value="CAZ80524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836333.1">
        <property type="nucleotide sequence ID" value="XM_002836287.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7N1"/>
    <dbReference type="STRING" id="656061.D5G7N1"/>
    <dbReference type="GeneID" id="9187003"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004642001"/>
    <dbReference type="eggNOG" id="KOG0725">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_0_1"/>
    <dbReference type="InParanoid" id="D5G7N1"/>
    <dbReference type="OMA" id="YMTGTDF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43180">
        <property type="entry name" value="3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE (AFU_ORTHOLOGUE AFUA_6G11210)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43180:SF63">
        <property type="entry name" value="DEHYDROGENASE_REDUCTASE FAMILY PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G03520)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ80524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="280" mass="29740" checksum="FDDB9B63AD430183" modified="2010-06-15" version="1">MSTSAGRLEGKACIVTGAAGGIGLETAILFAQNGGSVILADLNDVGLQAAKRKVEALIPGAKFKIFKCDVSNEENVKALVAEADEWGGVDVIFNNAGIMHNDDGDAISTSEDVWDLTHKINVKGVWYGCKHAVLSFRKHKKTKGSIINTASVVALVGSATAQVAYTASKGAVLALTRELAMVHAREGFRFNSLCPAPLNTPLLQDFLGADQAKRARREIHFPMGRFGEAIEQAHMVVFLASDESSFVTGQEMVVDGGMTKAYVTPKGPAVPPPLNNAFDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7N7</accession>
    <name>D5G7N7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00004655001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 2/4.</text>
    </comment>
    <dbReference type="EMBL" id="FN430031">
        <property type="protein sequence ID" value="CAZ80530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836339.1">
        <property type="nucleotide sequence ID" value="XM_002836293.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7N7"/>
    <dbReference type="FunCoup" id="D5G7N7">
        <property type="interactions" value="102"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G7N7"/>
    <dbReference type="GeneID" id="9187009"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004655001"/>
    <dbReference type="eggNOG" id="KOG2436">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4354">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006384_4_0_1"/>
    <dbReference type="InParanoid" id="D5G7N7"/>
    <dbReference type="OMA" id="KATICDS"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00107"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003991">
        <property type="term" value="F:acetylglutamate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003942">
        <property type="term" value="F:N-acetyl-gamma-glutamyl-phosphate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:L-arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04252">
        <property type="entry name" value="AAK_NAGK-fArgBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd24149">
        <property type="entry name" value="AGPR_N_ARG5_6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04263">
        <property type="entry name" value="DUF619-NAGK-FABP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000029">
        <property type="entry name" value="Bifunctional acetylglutamate kinase/N-acetyl-gamma-glutamyl-phosphate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1160.10:FF:000011">
        <property type="entry name" value="N-acetyl-gamma-glutamyl-phosphate reductase, variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004662">
        <property type="entry name" value="AcgluKinase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041734">
        <property type="entry name" value="NAGK-fArgBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000534">
        <property type="entry name" value="Semialdehyde_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006855">
        <property type="entry name" value="Vertebrate-like_GNAT_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00761">
        <property type="entry name" value="argB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23342">
        <property type="entry name" value="N-ACETYLGLUTAMATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23342:SF0">
        <property type="entry name" value="N-ACETYLGLUTAMATE SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04768">
        <property type="entry name" value="NAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01118">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00859">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51731">
        <property type="entry name" value="GNAT_NAGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="8">
        <location>
            <begin position="340"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ80530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="655" mass="71678" checksum="A99B4639E89511BA" modified="2010-06-15" version="1">MLSGLRNSSRRSVRLPTFRNLRVIRPAAPIPSSSSTCPLRITASSYATTSLSPSPLSTRSTVIQLLNNIGSKREVEQYLSHFTSVQSQQFAVIKVGGAIITEHLDSLSNSLSFLNHVGLFPVVVHGAGPQLNKMLEAAGVEPQFEDGIRVTDGKTLALARQLFLDENLKLVEALEKLGVRARPITGGVFQADYLDRKKYDLVGKITSVDKRPIEAAIKAGCLPILTSTAETAEGQLLNVNADIAAGELARALQPLKIVYLSEKGGLFNGETNEKISTINLDEEYAGLMEQWWCRFGTRLKIKEIKELLDGLPRTSSVAIIATEDLQKELFTDTGAGTLIRRGNKLTAMSSIEGFEDRTRLEEVLGREKNDVSEYLSFLQTTKFKAYADEPLEVLAIVLPSESTNEPARLDFFSSTKAGWLGNVADNVFNLIKKEYPRLVWSVRQGDGQLGWHFDKSQGSFNHGGQVHFWYGYEGPEEVKSLIGGFLADAGSRESGKSEGAVTGVKQQVRGFASTNPNPPLGSSNATNNKPSKVALIGARGYTGQALITLINSHPHFDLTHVSSRELAGKKLEGYTKDGKEIVYQNLGVEDIRRMEENGEVDCWVMALPNGVCKPFVDAVEDVGKGKSVIVDLSADFRFDSKWTYGLPGILCPERP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7V3</accession>
    <name>D5G7V3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00002668001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430032">
        <property type="protein sequence ID" value="CAZ80596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836405.1">
        <property type="nucleotide sequence ID" value="XM_002836359.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7V3"/>
    <dbReference type="FunCoup" id="D5G7V3">
        <property type="interactions" value="377"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G7V3"/>
    <dbReference type="GeneID" id="9181415"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002668001"/>
    <dbReference type="eggNOG" id="KOG2551">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051938_2_0_1"/>
    <dbReference type="InParanoid" id="D5G7V3"/>
    <dbReference type="OMA" id="EEPRGWW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019748">
        <property type="term" value="P:secondary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005645">
        <property type="entry name" value="FSH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050593">
        <property type="entry name" value="LovG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48070">
        <property type="entry name" value="ESTERASE OVCA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48070:SF6">
        <property type="entry name" value="ESTERASE OVCA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03959">
        <property type="entry name" value="FSH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine hydrolase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ80596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="251" mass="27410" checksum="A872F7F98E0A5A47" modified="2010-06-15" version="1">MSKPVKLLFLHGYTQSGPLFSAKTKALEKALLKSLPPQSALYYPTAPHPLSPSDLPGDPSSPDAPTAPIENYAWWRRNGETGEYLGIDETWNFLSSYLDANGPFDGVVGFSQGAALAVMLVSLLERELSRKTPESFSTTHSPLRFGVCYSGFRATGNYDYFYEPKIRTPVLHVLGSLDTVVDEERSLKLSRACVGGEERKVYHPGGHYLPAGKQYANMLLAYILEHTKAPGGEEKGVSVEVVDIPSPSTVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7Y1</accession>
    <name>D5G7Y1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="11">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00002629001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430033">
        <property type="protein sequence ID" value="CAZ80624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836433.1">
        <property type="nucleotide sequence ID" value="XM_002836387.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7Y1"/>
    <dbReference type="FunCoup" id="D5G7Y1">
        <property type="interactions" value="1027"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G7Y1"/>
    <dbReference type="GeneID" id="9181443"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002629001"/>
    <dbReference type="eggNOG" id="KOG0092">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_10_2_1"/>
    <dbReference type="InParanoid" id="D5G7Y1"/>
    <dbReference type="OMA" id="DEEGLMW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016050">
        <property type="term" value="P:vesicle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01860">
        <property type="entry name" value="Rab5_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000464">
        <property type="entry name" value="GTP-binding protein ypt5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0636">Prenylation</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ80624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="218" mass="23591" checksum="5FC4CB078CD77CD9" modified="2010-06-15" version="1">MSHARASTQSQRFAQFKLVLLGESAVGKSSLVLRFVKDQFDDYRESTIGAAFLTQTIALDETTTIKFEIWDTAGQERYKSLAPMYYRNANCAVVVYDITQASSLDKAKAWVKELQRQANENIVIALAGNKLDLALPTGSPSKRAVETAEAEAYAREAGLLFFETSAKTAENVKELFTAIAKKLPLDQQGPRGTTRTGAAGGRGVNLSGANAAQQGCNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5G800</accession>
    <name>D5G800_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00002749001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DAMOX/DASOX family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430035">
        <property type="protein sequence ID" value="CAZ80643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836452.1">
        <property type="nucleotide sequence ID" value="XM_002836406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G800"/>
    <dbReference type="STRING" id="656061.D5G800"/>
    <dbReference type="GeneID" id="9184116"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002749001"/>
    <dbReference type="eggNOG" id="KOG3923">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034311_1_0_1"/>
    <dbReference type="InParanoid" id="D5G800"/>
    <dbReference type="OMA" id="DLWELQP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003884">
        <property type="term" value="F:D-amino-acid oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019478">
        <property type="term" value="P:D-amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006181">
        <property type="entry name" value="D-amino_acid_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023209">
        <property type="entry name" value="DAO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11530">
        <property type="entry name" value="D-AMINO ACID OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11530:SF16">
        <property type="entry name" value="D-AMINO ACID OXIDASE (AFU_ORTHOLOGUE AFUA_5G11290)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000189">
        <property type="entry name" value="D-aa_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51971">
        <property type="entry name" value="Nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00677">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="7">
        <location>
            <begin position="6"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="46"/>
            <end position="47"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>D-dopa</name>
            <dbReference type="ChEBI" id="CHEBI:149689"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="337"/>
        </location>
        <ligand>
            <name>D-dopa</name>
            <dbReference type="ChEBI" id="CHEBI:149689"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000189-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ80643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="365" mass="39698" checksum="64C87F18950D2F15" modified="2010-06-15" version="1">MSEKSKVAVVGAGVAGLTTALLLSKRGHSVAVVAKHMPGDYDIEYTSPWAGASYLPVSAPGTREAEWDQITFAELYRLTTQVPEAGIHFQDVITYRREKDKGSVTADWFSELLKERPWFANIVPEFRTIPKADLPPSVDFGTRWKSMCINPAIYLPYLLSQCLKRQVRFRRGTLTHICEAVNFHPEYTSCSDIAVVNCTGLGSYTLSGVTDSSLTPARGQIVLVRNTAPAIIEVSGTDDGEDEVTYIMTRAAGGGTVLGGTYQKGNWSSAPDEATAERIKKRAVEWCPELVGKGEGVEGLDVIRHGVGLRPLRVGGARVEREVIGGARVVHNYGAGGFGYQASYGMAEEAVRLVEESLEEVKARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8C4</accession>
    <name>D5G8C4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00004739001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sestrin family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430042">
        <property type="protein sequence ID" value="CAZ80767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836576.1">
        <property type="nucleotide sequence ID" value="XM_002836530.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8C4"/>
    <dbReference type="STRING" id="656061.D5G8C4"/>
    <dbReference type="GeneID" id="9188369"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004739001"/>
    <dbReference type="eggNOG" id="KOG3746">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_300746_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8C4"/>
    <dbReference type="OMA" id="NWSTSEV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070728">
        <property type="term" value="F:L-leucine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016684">
        <property type="term" value="F:oxidoreductase activity, acting on peroxide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071233">
        <property type="term" value="P:cellular response to L-leucine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990253">
        <property type="term" value="P:cellular response to leucine starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016239">
        <property type="term" value="P:positive regulation of macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901031">
        <property type="term" value="P:regulation of response to reactive oxygen species"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006730">
        <property type="entry name" value="Sestrin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12474">
        <property type="entry name" value="P53 REGULATED PA26 NUCLEAR PROTEIN SESTRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12474:SF0">
        <property type="entry name" value="SESTRIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04636">
        <property type="entry name" value="PA26"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="AhpD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="46"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="252"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="335"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="568"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="657"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="828"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="254"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="280"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="351"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="568"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="663"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="863"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="880"/>
            <end position="890"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ80767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="995" mass="111380" checksum="D0D03169D9644F63" modified="2010-06-15" version="1">MANLFRHGLFNCFFDDEPILRTNALRILGLVVDHWTLAWQDYLRTSSGSSMSPRLARPPLVRQQSNGSGASPAPPSSRNMLRRDSSFKMHFEDVFGVDGDDSSTLDHLGRKDSIYNSFLPLTPERDDGWTTLSGDFAVSPTDASGGFADGAVRFGNKWLAELDFANRDLAWTGHQVADETWGVEEMKTNLENFYSWLLRFSIDCPFKDVRKECRAILQRAEKAGARLPEVPVRGPSFFIPLNETIGLNFGPWTPATSNPKSPTSFDIPSEDEEDCISFSPRESNGNMSPASGIMIPDPNKGSANRERPRSTSRGRTPIGSLNRGTRRTTVSIVSSLFQTPPSPPNGVGTSHKVKRRQARRPMPKYLPKPKPPSDEVRALQVNAYMRTGRVGNLNKILFFFPRFAEINRETTDLFLSDDSGNGPLPQKWRIYLGIISSAEKKCQYYLSLLSEKFLTVRGDRQWLKGLHYTPPKLQRISKLNMLLAHRPWTITPDDIKELVRGEEGGNMDPEQRWTMAEVVQAICIMSFYHSKSAMALAWAILPEADLLGGTVKTMEKYTPVAPFGSLATTSIPTEPSENSGFVKRDEESGKSGVCRKRQQQVAEGQEPMELPEKWRKTPNGGIPFISDPNSQTSPLLSSSKPVDVPTASLIKTQATLGTVKERERHHRRTKSQKRRKSFEECAASDDAPPPATCLGGFSLSAPNSYVPFSSLSNGRPLPAQARDFLISPSSSTSSLFDTSLLGDQVPANIIVEDYARFLHPESLDHEHKDFDPNSYEYEFLPMEEFEWERNASSIISDYLDGVVELLDRYFKEAQEADGEEDTLFSAAVETLPPRKRLNDEPSSGRGDDRPSRAPCDGEFDDLDSSHDHNLSERHDRIDRSFNSSGRSTSSPPRYGFEPRSFKEAAWFYSLRLLGIQKDDFRYSEIKVLLDKGARRYLKRICAQPDMVSLADWAGVGQGIGVLKCEEKCLLSLLACQAQFMGEMLWGIRAVSEYIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8V3</accession>
    <name>D5G8V3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00004854001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430053">
        <property type="protein sequence ID" value="CAZ80946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836755.1">
        <property type="nucleotide sequence ID" value="XM_002836709.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8V3"/>
    <dbReference type="STRING" id="656061.D5G8V3"/>
    <dbReference type="GeneID" id="9186455"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004854001"/>
    <dbReference type="HOGENOM" id="CLU_2759633_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8V3"/>
    <dbReference type="OMA" id="ICTWCAN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="2">
        <location>
            <begin position="10"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ80946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="70" mass="7746" checksum="1A73DAF0E2DC33FC" modified="2010-06-15" version="1">MEAMKLDCECKICFGQIADTLLLPCSHLAICTWCANQMGIKPISELHFGPPIHCPVCRVAVSSRIKVFRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8V6</accession>
    <name>D5G8V6_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Squalene monooxygenase</fullName>
            <ecNumber evidence="16">1.14.14.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">GSTUM_00004857001</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Catalyzes the stereospecific oxidation of squalene to (S)-2,3-epoxysqualene, and is considered to be a rate-limiting enzyme in steroid biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21 16">
            <text>squalene + reduced [NADPH--hemoprotein reductase] + O2 = (S)-2,3-epoxysqualene + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25282"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15440"/>
            <dbReference type="ChEBI" id="CHEBI:15441"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="1.14.14.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 16">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="15">Steroid metabolism; ergosterol biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 16">Endoplasmic reticulum membrane</location>
            <topology evidence="3 16">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the squalene monooxygenase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.17" evidence="16"/>
    <dbReference type="EMBL" id="FN430053">
        <property type="protein sequence ID" value="CAZ80949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836758.1">
        <property type="nucleotide sequence ID" value="XM_002836712.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8V6"/>
    <dbReference type="FunCoup" id="D5G8V6">
        <property type="interactions" value="157"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G8V6"/>
    <dbReference type="GeneID" id="9181232"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004857001"/>
    <dbReference type="eggNOG" id="KOG1298">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026390_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8V6"/>
    <dbReference type="OMA" id="AKRTFYW"/>
    <dbReference type="UniPathway" id="UPA00767">
        <property type="reaction ID" value="UER00752"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004506">
        <property type="term" value="F:squalene monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000166">
        <property type="entry name" value="Squalene monooxygenase Erg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013698">
        <property type="entry name" value="Squalene_epoxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040125">
        <property type="entry name" value="Squalene_monox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10835">
        <property type="entry name" value="SQUALENE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10835:SF0">
        <property type="entry name" value="SQUALENE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08491">
        <property type="entry name" value="SE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 16" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 16" id="KW-0274">FAD</keyword>
    <keyword evidence="5 16" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0492">Microsome</keyword>
    <keyword evidence="12 16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="14" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="17"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="413"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="440"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="17">
        <location>
            <begin position="16"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="Squalene epoxidase" evidence="18">
        <location>
            <begin position="168"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00029435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU367121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF08491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAZ80949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="466" mass="50916" checksum="67DCB9978327A7D6" modified="2010-06-15" version="1">MTDAAAKPEKGDNSYDVAIVGAGIIGCSLAFALGEQGRRVLLLERDLSEPDRIVGELLQPGGVQALEKLGLRDCLEGIDAIPVYGYQCIYHGEGVTIPYPRDPDGRAPEGRSFHHGKFIMNLREAAGNAPNVTIVETTAREIIKDESSGQVLGVICKKKGFDELDYYFASLTVSADGYASNFRKRCIDRKPQVRSHFFALKLKDAKLPSPGYGHVVMGNNPPVLLYQIGTHDTRALIDVPRAPSAASGGIRGHLMNVVLPDLPECIQPSFKEALKADRFPSMPNSFLPASTNTTPGMMLLGDAMNMRHPLTGGGMTVAFNDVVLISDLLSPENIPDLADTDLVLAQLSRFHWERKSLTSVINILAQALYALFAANDNKLRTLQKGCFKYFQRGGQCIDGPVGLLAGITRQPMVLFFHFFAVAFYSIYILFASLSPANWPVAFINSFGVFYKACVVIFPYIFSEAKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5G939</accession>
    <name>D5G939_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00004970001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PET191 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430053">
        <property type="protein sequence ID" value="CAZ81032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836841.1">
        <property type="nucleotide sequence ID" value="XM_002836795.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G939"/>
    <dbReference type="FunCoup" id="D5G939">
        <property type="interactions" value="262"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G939"/>
    <dbReference type="GeneID" id="9184319"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004970001"/>
    <dbReference type="eggNOG" id="KOG4114">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_138069_0_0_1"/>
    <dbReference type="InParanoid" id="D5G939"/>
    <dbReference type="OMA" id="GNMPVTY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018793">
        <property type="entry name" value="Cyt_c_oxidase_assmbl_Pet191"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28627">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28627:SF1">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10203">
        <property type="entry name" value="Pet191_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="77"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="79"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ81032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="110" mass="11872" checksum="AE9F6495033C9782" modified="2010-06-15" version="1">MPSSCKDIRAALAQCLENSDCVMVDRNKPADCLRYPLVETLPTRCQRLKKGYGECKRGMVDMRKRFRGNYPVALSVENEGGGGSRQLYGGGSALSDPPSGTKAEGKEGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9I2</accession>
    <name>D5G9I2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Queuine tRNA-ribosyltransferase accessory subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Queuine tRNA-ribosyltransferase domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00003337001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Non-catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Heterodimer of a catalytic subunit and an accessory subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the queuine tRNA-ribosyltransferase family. QTRT2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430062">
        <property type="protein sequence ID" value="CAZ81175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836984.1">
        <property type="nucleotide sequence ID" value="XM_002836938.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9I2"/>
    <dbReference type="STRING" id="656061.D5G9I2"/>
    <dbReference type="GeneID" id="9185351"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003337001"/>
    <dbReference type="eggNOG" id="KOG3909">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037350_1_0_1"/>
    <dbReference type="InParanoid" id="D5G9I2"/>
    <dbReference type="OMA" id="VPHIAHD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008479">
        <property type="term" value="F:tRNA-guanosine(34) queuine transglycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.105">
        <property type="entry name" value="Queuine tRNA-ribosyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03043">
        <property type="entry name" value="QTRT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028592">
        <property type="entry name" value="QTRTD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050852">
        <property type="entry name" value="Queuine_tRNA-ribosyltrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036511">
        <property type="entry name" value="TGT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002616">
        <property type="entry name" value="tRNA_ribo_trans-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00449">
        <property type="entry name" value="tgt_general"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46064">
        <property type="entry name" value="QUEUINE TRNA-RIBOSYLTRANSFERASE ACCESSORY SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46064:SF1">
        <property type="entry name" value="QUEUINE TRNA-RIBOSYLTRANSFERASE ACCESSORY SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01702">
        <property type="entry name" value="TGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51713">
        <property type="entry name" value="tRNA-guanine transglycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="tRNA-guanine(15) transglycosylase-like" evidence="6">
        <location>
            <begin position="29"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="322"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="324"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="353"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ81175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="412" mass="45126" checksum="576B11EE9F4DB584" modified="2010-06-15" version="1">MAAPANSRIVFDLQTAGEGTATGGGNNGSARIGRFANARGGVSFDTPSFVAQTSRGAVPHLSGDNLRDHTDVKVAYVALEDFIEKPHGNVPVCNHPSTLRDFISHPSNSLLMLAPRRSPPVTTAASNTDASLAILTSVGFRALKVDDYFNAVLKLRPDIAISPSDLPHQTPGKNRGPKMVFRTELWLGELINRNASNISIFAPVLPLDIERQSYYLGYLKEHISSLSGLALYDARLAVDLPSDLEGLPRLSLDQAQTPHQVLRQVSWGVDMLDLAFLTQMTDAGISLTFQFPGEASGKKEALGVDLWKKEYGTSIEPLAAVCKCYTCRKHHRAYVQHLLTAKEMTAWVLLQIHNAKIISNFFTAVRESIARCTFEEDCKIFAETYAEEFPEKTGHGPRYHFPSVGPRGALLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9M1</accession>
    <name>D5G9M1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00003320001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430063">
        <property type="protein sequence ID" value="CAZ81214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837023.1">
        <property type="nucleotide sequence ID" value="XM_002836977.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9M1"/>
    <dbReference type="STRING" id="656061.D5G9M1"/>
    <dbReference type="GeneID" id="9185357"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003320001"/>
    <dbReference type="eggNOG" id="ENOG502RG9A">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_631918_0_0_1"/>
    <dbReference type="InParanoid" id="D5G9M1"/>
    <dbReference type="OMA" id="MMVDGSE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014840">
        <property type="entry name" value="HRD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08729">
        <property type="entry name" value="HUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hpc2-related" evidence="2">
        <location>
            <begin position="328"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="297"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="357"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="16"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="201"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="300"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="315"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="366"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="404"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ81214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="434" mass="46374" checksum="15D3671B62D0E5C6" modified="2010-06-15" version="1">MSTFGSPSAMTRGVQSSPSSKPASPTAGNVSDKSANNSNRGTHTNNHPLSTSSPLHRQPKFPQDVVDSSGRITNNHNGKKSAPHTGPSRPRQEIIELDSDTEPNSASGKLNSSIAPSARNRSLLGQPFDPVRSGSTAKSGHRASASPSISSLIDPQPLTEPHAAALSSDKQFNSSFASDTAVGNGVRVEKLPRSGEVGDSLNRSPKSKNGTAPSSAAASPKPRPSLPSTSGNGLLSGVLPGTTAATSEACIPNIYIHVPLNGEHNKYVNFAKLAEERYGWAALNPKLARARENMMKGDSGDEMMVDGSESESNIEMDKPMDSEGGKRKKRRHQDIYDKNDPFIDDTEMLWEEQAAASKDGFFGEKPQIERADGTVKRGRGRGRGGTTRGTAAARGPTIRKPRLTKKEKERMEKEKEEREKTFFQVTSVKPPGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9W5</accession>
    <name>D5G9W5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">urease</fullName>
            <ecNumber evidence="2">3.5.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00005102001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Ni cation</name>
            <dbReference type="ChEBI" id="CHEBI:25516"/>
        </cofactor>
        <text evidence="7">Binds 2 nickel ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nitrogen metabolism; urea degradation; CO(2) and NH(3) from urea (urease route): step 1/1.</text>
    </comment>
    <comment type="PTM">
        <text evidence="6">Carbamylation allows a single lysine to coordinate two nickel ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the metallo-dependent hydrolases superfamily. Urease alpha subunit family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.5" evidence="2"/>
    <dbReference type="EMBL" id="FN430064">
        <property type="protein sequence ID" value="CAZ81308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837117.1">
        <property type="nucleotide sequence ID" value="XM_002837071.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9W5"/>
    <dbReference type="STRING" id="656061.D5G9W5"/>
    <dbReference type="MEROPS" id="M38.982"/>
    <dbReference type="GeneID" id="9188551"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005102001"/>
    <dbReference type="eggNOG" id="ENOG502QPKB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000980_2_0_1"/>
    <dbReference type="InParanoid" id="D5G9W5"/>
    <dbReference type="OMA" id="DTMDGVH"/>
    <dbReference type="UniPathway" id="UPA00258">
        <property type="reaction ID" value="UER00370"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009039">
        <property type="term" value="F:urease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043419">
        <property type="term" value="P:urea catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00375">
        <property type="entry name" value="Urease_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01953">
        <property type="entry name" value="Urease_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011612">
        <property type="entry name" value="Urease_alpha_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005848">
        <property type="entry name" value="Urease_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017951">
        <property type="entry name" value="Urease_asu_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029754">
        <property type="entry name" value="Urease_Ni-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050069">
        <property type="entry name" value="Urease_subunit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009686">
        <property type="entry name" value="PRK13207.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01792">
        <property type="entry name" value="urease_alph"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33569">
        <property type="entry name" value="UREASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33569:SF1">
        <property type="entry name" value="UREASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00449">
        <property type="entry name" value="Urease_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01752">
        <property type="entry name" value="UREASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01120">
        <property type="entry name" value="UREASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51368">
        <property type="entry name" value="UREASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 7" id="KW-0533">Nickel</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Urease" evidence="11">
        <location>
            <begin position="121"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8 9">
        <location>
            <position position="297"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="via carbamate group" evidence="7">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="via carbamate group" evidence="7">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="337"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-carboxylysine" evidence="6">
        <location>
            <position position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR611612-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR611612-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR611612-52"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ81308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="542" mass="58214" checksum="6EA1528EB8712744" modified="2010-06-15" version="1">MFGPTTGDLVRLGDTELWIKVEKDYTVYGDECKFGGGKSLREGMGQASGRSDAETLDLVITNALVVDYTGIYKADVGVKGGFIAGIGKAGNPDMMEGVHEGMVIGSNTDVIAGEGKILTYGGFDSHIHLICPQQAEEALASGITTMLGGGCGPTTGTNATTCTPSMTQIRQMLQADSYPEGLVEQCRAGVVGLKLHEDWGTTPAAIDKCLSVCDEYDIQCLIHTDTLNESGFVEQTIGAFKDRTIHTYHTEGAGGGHAPDIICVVEHPNVLPSSTNPTRPLTRNTVAEHLDMLTVCHHLSKEIPEDVAFAESRIREETIAAEDILHDMGAISMMSSDSQAMGRCGEVILRTWKTASKNKEQIGPLEEDSELGADNTRVKRYISKYTINPAVAHGMSHLVGSVELGKLADLVLWSPANFGAKPTQVLKSGLIAYAQMGDPNASIPCVQPVLGRQMFAARVPEACITFVSRVSLETGTVQTYGLKKRVEPILGCRGISKKDMKYNDAMPKMKVDPENFTVVADGVTLKSAVSDKLPLTQGYFMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5GA59</accession>
    <name>D5GA59_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00005146001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430075">
        <property type="protein sequence ID" value="CAZ81413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837222.1">
        <property type="nucleotide sequence ID" value="XM_002837176.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GA59"/>
    <dbReference type="FunCoup" id="D5GA59">
        <property type="interactions" value="787"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GA59"/>
    <dbReference type="GeneID" id="9185695"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005146001"/>
    <dbReference type="eggNOG" id="KOG1104">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013816_0_0_1"/>
    <dbReference type="InParanoid" id="D5GA59"/>
    <dbReference type="OMA" id="CAAEGLM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005846">
        <property type="term" value="C:nuclear cap binding complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000339">
        <property type="term" value="F:RNA cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000035">
        <property type="entry name" value="snRNA cap binding complex subunit (Gcr3)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027159">
        <property type="entry name" value="CBP80"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015172">
        <property type="entry name" value="MIF4G-like_typ-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015174">
        <property type="entry name" value="MIF4G-like_typ-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12412">
        <property type="entry name" value="CAP BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12412:SF2">
        <property type="entry name" value="NUCLEAR CAP-BINDING PROTEIN SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09088">
        <property type="entry name" value="MIF4G_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09090">
        <property type="entry name" value="MIF4G_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIF4G-like type 1" evidence="2">
        <location>
            <begin position="334"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="domain" description="MIF4G-like type 2" evidence="3">
        <location>
            <begin position="542"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ81413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="813" mass="90500" checksum="F96A402F79BA8715" modified="2010-06-15" version="1">MGDYYRGREEGRGGGRGGGPRRSQKRRHRDDDDDDKEYRRSRPYEREDPASRLRRDTISIAESPLTKIEETINLIANTFASNCDSEELRNTFLNTLLSLVVEQPFKIPFLTAAVLVINSRNAEAGKLILDEFGKAAQAYLTEGFLTSAKLVLRFLAGLQGMVEGEGLYFVLGGIVDQAAGYLAAEEVGLALDLVWITLITVPYILASSRSDNSLASELLKKTSSITEVKHRMQSLLSPFTGGYAPYDARAQGTHNARILQLNLEACLGSEPSRPFHGVGCLPRPYESFLQELEGATKHPMPEITLPSDYPVDKSAIMPHVYFSVFADPNVETVPPPLELASTLFRDAIVDTIDTLDCNRLVVSRFLIEIDCYFSPNAFIKRAVPFDRVIETAGPEGSTWKPEDVAVDAIFSQLYKLPGPNHKLVYYHSILTELCKLAPAAVAPSLGRAIRFMYKNLESMDTELIYRFVDWFSHHLSNFGFTWKWAEWNENLTLPDVHPKKAFILGALEKEIRLSFATRIKGTIPAPYKELISEAKEKEQPHFKYDSEDEPFCKEAKEVLHAVADKKEDAEVDAILAKIVELAKESGVADPLFVARDAYVTAICHIGAKSLSHVLSCIERCKEKLLAIGNESPDARRQIVGSVLSYWEDQPGIGANVVDKLLNYSIVTPLSVVEWVLHDAGNKALSHTHAWEMVSTTINKVNTRVRQMVAARRVMSINDYTEEQLAVYAQTLNAAEIEQGVLLNAVTDALTAMSDSSDEMNGGIDVESKAWMQWWAKGWLRAFSRKFGVAEGTVVEQPVGGPSARLDPEDMDTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAB8</accession>
    <name>D5GAB8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="15">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">GSTUM_00005225001</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430075">
        <property type="protein sequence ID" value="CAZ81472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837281.1">
        <property type="nucleotide sequence ID" value="XM_002837235.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5GAB8">
        <property type="interactions" value="169"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GAB8"/>
    <dbReference type="GeneID" id="9185754"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005225001"/>
    <dbReference type="eggNOG" id="KOG0054">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_27_6_1"/>
    <dbReference type="InParanoid" id="D5GAB8"/>
    <dbReference type="OMA" id="RRRYILW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18596">
        <property type="entry name" value="ABC_6TM_VMR1_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18604">
        <property type="entry name" value="ABC_6TM_VMR1_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000054">
        <property type="entry name" value="ABC bile acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000565">
        <property type="entry name" value="ABC bile acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000825">
        <property type="entry name" value="ABC bile acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF353">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN_PERMEASE VMR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="190"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="223"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="327"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="355"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="434"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="837"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="975"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="999"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="14">
        <location>
            <begin position="193"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="13">
        <location>
            <begin position="534"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="14">
        <location>
            <begin position="828"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="13">
        <location>
            <begin position="1063"/>
            <end position="1287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="766"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="772"/>
            <end position="784"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAZ81472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1327" mass="148001" checksum="25FFCDBA9B0DCACE" modified="2010-06-15" version="1">MNTEAIRHIERLPFMASLITWSYIFVLATLRLVFSGSSSYDLPPLWYHTSFLYMLNFLLIAVSFRSALIHPESAVSQALIVTRFVLVSVLCVIAVTSRAGNKVVTQEIVDGLEPSREPLASLFSLASFSWVDPLIWKGYWKSFELHDVWNLRNDDIAVTVFLDTNCLNRKAHTLAWTLAKHFKRELAVQAAWAVFYSMFTFAPTLLLRIILQYVKSPEDTPKNVAWLFVVLLFVTATVQAIGSGQALYIGRRICIRLHSVIVGEIYAKTLRRRAAAGADRALGPKGKHDGKKDSEGKAIATEDNGEQANAGAIINLMSVDSFKVAEVCAYLHFLVAGVPVQVAIAVVLLYQILGWSSIAGIVVMFFLLPINYFISSRFSQIQKKIMAATDERIHTTNEALQNIRVIKFFAWEARFAQVVGQSRSAELLQLRNRYILWAVAATSWYGSPIVITFLSFLCYTVVEGKTLDSPIAFTALSLFNVLRVPLDQLADMVTNVLQTKVSIDRVEEYLSEEETEKYHQLKQPDEVDPNAPLIGFKDATFSWGNRKQIKNKGMGSAFQLQDLNIEFSPGYLNVIAGPTGSGKTSLLMALLGEMTYIKGSVHLPGAHSREDLIPDPVTGLTESVAYCAQQAWLVNDTIKNNILFASPYDEERYHSVLIACSLERDLQILDHGDETEVGEKGITLSGGQKQRISLARALYSNSSHILLDDCLSAVDSHTSKWIYDYCIMGPLMAGRTCILVTHNVALCVPEAKHVVVLDNGRLLRSGIRSKPHSQMPSRPSTNGEESAAKKDKISGEETKALGSVDWRVYYLYLRSMGPCARNAPSITIAENTDDINLVYYLGVYALIGFAYTFVSFLREAIVFYGSLIASKNIHNQLLYNVMRAKFRFFDSTPLGRIINRFSKDMEAVDQERLESIQRSPLYQHFGETLSGISTIRAYGDERRFVRDNLSKIDNHHRPFFYLWACNRWLGFRVDIAGALVSFFAGAFVVLSVGKIDAGLAGLSLTYAITFTDNILWLVRLYAMNEQNMNSVERIREYLEVGQEAADIIPDNRPVSTWPEKGGVAFENYSTRYRPGLDLVLKNVTFNIKPLEKVGIVGRTGAGKSSLALALFRSLEAETGKIVIDGMDISTIGLQDLRRSITMVPQDPTLFTGTIRSNLDPFGVHTDGEVFNALKRNVFLDLHSPISESGNNLSQGQKQLLCLARALLKDPVVLLMDEATASIDYSTDTKIQATIRELKSTTITIAHRLNSIIFYDKVLVLDQGGVKEFDCPHNLLQKKDSLFREMCESSGELGALEEMAKRAFMGTGTLIEIAQDDSNDTNPNDVVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAI9</accession>
    <name>D5GAI9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00003608001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Spindle pole body</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430078">
        <property type="protein sequence ID" value="CAZ81532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002837295.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAI9"/>
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        <property type="interactions" value="59"/>
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    <dbReference type="GeneID" id="9187472"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003608001"/>
    <dbReference type="eggNOG" id="KOG1820">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008401_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAI9"/>
    <dbReference type="OMA" id="HNFGCKI"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="term" value="C:cytoplasmic microtubule"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
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        <property type="term" value="C:mitotic spindle microtubule"/>
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        <property type="term" value="F:microtubule plus end polymerase"/>
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        <property type="entry name" value="ARM-like"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR048492">
        <property type="entry name" value="Stu2_CTS"/>
    </dbReference>
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        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045110">
        <property type="entry name" value="XMAP215"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048491">
        <property type="entry name" value="XMAP215_CLASP_TOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12609">
        <property type="entry name" value="MICROTUBULE ASSOCIATED PROTEIN XMAP215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21042">
        <property type="entry name" value="Stu2_CTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21041">
        <property type="entry name" value="XMAP215_CLASP_TOG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TOG" evidence="6">
        <location>
            <begin position="1"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="6">
        <location>
            <begin position="265"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="232"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="418"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="489"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="716"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="625"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="526"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="573"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="740"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="768"/>
            <end position="779"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ81532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="837" mass="91667" checksum="5EA683453BC3F2B8" modified="2010-06-15" version="1">MAEEEPDYSNLPLTDRAVHKVWKVRKAAYEEAAAEFAKSPDEGAPCFRDWLMDSGLWKKIVLDSNVAAQQEGITALCAFLQYGGVNACLRSRSHTISPLVEKGLSSTRAGTKQKSLEAVLLYIELDTPGPVLDELAPYLSHKMPKIIAATITTFTSIYSAFGARTVDPKPVLKALPALFGHADKNVRAEATKLAIELYKWLKDAMKPMFFNDLKPVQQKELEEAFEKVKDESPKQERLLRSQQAMAGGEEEQEEEEVEADVDAFDLAEPVDVLSKLPSQFHEMIASTKWKDRKDALESLFALLNVPRIRDGDFNEIIRALAKSMKDANVVVVTVAANCVELLANGLRKAFGRHRSSIMTPILERLKEKKPTVVEALAKALDAIFGATSLTDCLEDILEYMKHKNPNVKLETLRFSRGYRNGTRPESKSSAEASGKLLSDTTAPARDGAAEAMGTLMKILGERQMNPFLDGLDEIRKVKIKEFFETAEVKAKEKPKPVAPPPAAKAPPMKKILGGKPGLKKKAPPVAAVPPPPTEEPQRPMNPPRAIPSKLNAPKAGPTGLRLKKPSGAGGSGALASPKRAAAPRAPSPEEEPPAPKVAFGGRGLASRPLAKEVAPAARAVDSGLSALEKAELEELRAEKDRWARQIQEEKAEKAKLFQEINELQLQNAQLIEDHTRDNLSIRAKEAQLVRARSDAETAEATVHKQQREMERLKRELARAVRPTSPAPTDISEHVYRDNGLNGSYNSGRNDSVGRGAGNRLSFASTFSGEDKENGYDRGSRMSPTLREGTESGRASAAGNRGGADSIESWKRAAEVTSQLKLRIEQMKAKQGLARNQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAU5</accession>
    <name>D5GAU5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00005311001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430086">
        <property type="protein sequence ID" value="CAZ81638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837447.1">
        <property type="nucleotide sequence ID" value="XM_002837401.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAU5"/>
    <dbReference type="STRING" id="656061.D5GAU5"/>
    <dbReference type="GeneID" id="9187806"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005311001"/>
    <dbReference type="HOGENOM" id="CLU_394933_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAU5"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="180"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="296"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="181"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="201"/>
            <end position="248"/>
        </location>
    </feature>
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        <location>
            <begin position="255"/>
            <end position="273"/>
        </location>
    </feature>
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        <location>
            <begin position="331"/>
            <end position="340"/>
        </location>
    </feature>
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        <location>
            <begin position="346"/>
            <end position="356"/>
        </location>
    </feature>
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        <location>
            <begin position="366"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="377"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="387"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="434"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="464"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="484"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="501"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="698" mass="78778" checksum="B030AE1985CC1B6A" modified="2010-06-15" version="1">MSPSLKRKSNPRAPHTPSKRLRTDGLFTPRVKAPTGASRSIPRASRYQPSSASPAKRTLDEEHVEQGLSSKVRILNNRECLYLLPSRKSLPVPVPALQRKVKPRSPVKDAIIARLSRRRAILLRKQKLERMMERMDSDNVYYFSEGLSDNIKLFTSRSPASFSSFSVEIADLDMDIEEPTGATTITSDSPSQSASDPESLVEEKIETDAPEEGATKKSGDADEELVRDPGSKPEERKEEEKDSMKDAEAEPEEEVLMKDDEAEEKKEDVKEASETLSTMDKLVSDRVKERLGEDGMICLNVKTKKVGEEESTAARKSPIKKKVKVVLPPTLEEEDEEEKELESPKKRNSRPSGLRKKREEADDSSDSEREDREEKPRRRSSRSSVRKKYEDSNPSDTEKDGDKREEEGEEATTHPDKMEEDADADAESTGSSGEDTDMSTDDEAPTETTEEEDVAMSEDGDDSGDSRDNSDEKKEDGGERQEKEDEADNEDNGEPSTLIKTDSKTSDEEVRKPKRVKALTLSSQHEEAEAVSTLKTPSSAASLRYNYSITKTPKSPRSPRLFKHDLVPSSPLSFSSFGKKKKALLKTEIERDASLWMLGRSSGLYPKRRRQLPFNPANVSRHAYRDSDSDDGEDMYTHHHHGFQSSRWETDEAEDDDVYLHVGWPWDGRGCYNPWMMEHGATLLEFCVRKLEGFNMAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAX1</accession>
    <name>D5GAX1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="13">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00005342001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430086">
        <property type="protein sequence ID" value="CAZ81664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837473.1">
        <property type="nucleotide sequence ID" value="XM_002837427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAX1"/>
    <dbReference type="FunCoup" id="D5GAX1">
        <property type="interactions" value="940"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GAX1"/>
    <dbReference type="MEROPS" id="C19.972"/>
    <dbReference type="GeneID" id="9187832"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005342001"/>
    <dbReference type="eggNOG" id="KOG2026">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016848_2_1_1"/>
    <dbReference type="InParanoid" id="D5GAX1"/>
    <dbReference type="OMA" id="QLRRFKC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
        <property type="term" value="P:spliceosomal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02669">
        <property type="entry name" value="Peptidase_C19M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033809">
        <property type="entry name" value="USP39"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF16">
        <property type="entry name" value="U4_U6.U5 TRI-SNRNP-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00290">
        <property type="entry name" value="ZnF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBP-type" evidence="12">
        <location>
            <begin position="92"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="11">
        <location>
            <begin position="214"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="44"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ81664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="550" mass="62515" checksum="A2B4DFF2950301FC" modified="2010-06-15" version="1">MSKRPAEETVGALEAQRQSTGPQTPKRARLEEVIDQEFVEKGSSRNGMILEASRPRAPAPTTDMDEDGDYEDDVAVGQLGVGKAQEGPSEGFSDLYLDTINRLLIKVDFEKLCSVSLSNINVYACLVCGKYFQGRGRSSHAYTHSLDQDHHVFINIASLKVYVLPESYEVQNKTLEDIKYVVNPTYTKVDVTKLDKEPLVRWDLGGKKYIPGFVGLNNIKQNDYMNVVVHALAHVAPLKNYFMLEDLSSRPELAQRFSILVRKIWNPRAFKSHVSPHELLQEISLRSSKRFTLTIQSDPVDFLSWFINNLHLSLGGSKTKPGTSIIQKVFQGRLKVESQTITARADASDRLRFEEAAEIKMDIVRFMMLTLDLPPKPIFKDEQDKNIIPQVPLTTILSKYDGLHSQELLGQRKRYRLLSPLPPFLIFHIKRFSKNKFVEERNPTIVTFPTRSLDMSPYVEPSEGGSSTEPIWYDLVANITHESVTTTSGKKEGDIRSVYKVQLVNKASGEWFQIQDLFVEEVARELLFTSESYVQIWQLRKEPKGKGTEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBC3</accession>
    <name>D5GBC3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00000413001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430090">
        <property type="protein sequence ID" value="CAZ81816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837625.1">
        <property type="nucleotide sequence ID" value="XM_002837579.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBC3"/>
    <dbReference type="FunCoup" id="D5GBC3">
        <property type="interactions" value="173"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GBC3"/>
    <dbReference type="GeneID" id="9184390"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000413001"/>
    <dbReference type="eggNOG" id="ENOG502QRKP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023943_1_0_1"/>
    <dbReference type="InParanoid" id="D5GBC3"/>
    <dbReference type="OMA" id="AHWKEDQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042175">
        <property type="term" value="C:nuclear outer membrane-endoplasmic reticulum membrane network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008298">
        <property type="term" value="P:intracellular mRNA localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039604">
        <property type="entry name" value="Bfr1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31027:SF2">
        <property type="entry name" value="LEBERCILIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31027">
        <property type="entry name" value="NUCLEAR SEGREGATION PROTEIN BFR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="135"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="398"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="507"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="99"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="237"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="435"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="446"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="521"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ81816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="576" mass="65335" checksum="5151BF9AF9FDA3B2" modified="2010-06-15" version="1">MLSCCTQKKTKPMIRTPILNFFCQALITTKPPQSLNNPPYLHGNSSDRLPLRNRVKKVRHKQTSTSHSTMTDIATPFAAALGTADIGKKFVQKPEKPDQTEFEKRLKEAQEEHDKVRQKLSEVKAKLDLVQVPGKNPLQNELREKLSDLRSQQSTKKNDRARIDEEIKTKDSSLKAKIKEFNARKEKIPYKTLEELEKAVKTLEKSVEKGDMTLVGERKALSEISTLQKQRKIFGALDADQKAIDEEREKLEELKAKRKDPDVQRLSDEYEETQGKLDAIKAEQDEAFGNLNKLRDERSSLYDQQKEKWQNIQDLKDDYYSARNAYREYEKEAWRIRREKKAKEDEEYRLAKKREAAQQKMEEASQKAYTSEILTCEGLVGYFDPSSAEAARKAKLLSEPRVLAAQPTRSVGGSAPSGKKLIRKDDREEGYFTGKPKKGKKGKKNAGTTSPNPETSPATPARSASGKINLPGGVVQELIKVDILPPSNKEDVQRVLKELRGKLAWYKDNQDQKTTENIAKAQKEIDRLEAEDFDGNEEKEVTNGTSTPNEEPAEAAIAESGEKGDETEEKAEEKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBI9</accession>
    <name>D5GBI9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Mitochondrial import receptor subunit TOM20</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Mitochondrial 20 kDa outer membrane protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Mitochondrial import receptor subunit tom20</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Translocase of outer membrane 20 kDa subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00005655001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tom20 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ81995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837804.1">
        <property type="nucleotide sequence ID" value="XM_002837758.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBI9"/>
    <dbReference type="FunCoup" id="D5GBI9">
        <property type="interactions" value="310"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GBI9"/>
    <dbReference type="GeneID" id="9185430"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005655001"/>
    <dbReference type="eggNOG" id="KOG4056">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1714649_0_0_1"/>
    <dbReference type="InParanoid" id="D5GBI9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005742">
        <property type="term" value="C:mitochondrial outer membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030943">
        <property type="term" value="F:mitochondrion targeting sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016031">
        <property type="term" value="P:tRNA import into mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.960.10:FF:000002">
        <property type="entry name" value="Mitochondrial import receptor subunit TOM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.960.10">
        <property type="entry name" value="Mitochondrial outer membrane translocase complex, subunit Tom20 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002056">
        <property type="entry name" value="MAS20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023392">
        <property type="entry name" value="Tom20_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12430">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12430:SF0">
        <property type="entry name" value="TRANSLOCASE OF OUTER MITOCHONDRIAL MEMBRANE 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02064">
        <property type="entry name" value="MAS20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00351">
        <property type="entry name" value="OM20RECEPTOR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47157">
        <property type="entry name" value="Mitochondrial import receptor subunit Tom20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00068548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00073975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00080405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ81995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="153" mass="16515" checksum="5109DF9A55F12ECC" modified="2010-06-15" version="1">MQTQTMIIIGATTLVTLGGGERDLLRLQTAQRPDLPQSPPPVSEEAPKRRMLIHAVAEAQAAGFPTDVEDKEAYFMREVAEGEGLCQEEDRALDAALCFYKALKVYPQPKDLISIYDKTVPKNVLDILAEMIATDGSIPVGGMSPPGSTTGVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBW6</accession>
    <name>D5GBW6_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Calcium-transporting ATPase 2</fullName>
            <ecNumber evidence="2">7.2.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">GSTUM_00005619001</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium. Transports the calcium to the vacuole and participates in the control of the cytosolic free calcium.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>Ca(2+)(in) + ATP + H2O = Ca(2+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18105"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="7.2.2.10" evidence="2"/>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ81966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837775.1">
        <property type="nucleotide sequence ID" value="XM_002837729.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBW6"/>
    <dbReference type="STRING" id="656061.D5GBW6"/>
    <dbReference type="GeneID" id="9185401"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005619001"/>
    <dbReference type="eggNOG" id="KOG0204">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002360_10_7_1"/>
    <dbReference type="InParanoid" id="D5GBW6"/>
    <dbReference type="OMA" id="WIFISIM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000018">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093:SF369">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093">
        <property type="entry name" value="CATION TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08282">
        <property type="entry name" value="Hydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="297"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="327"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="367"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase C-terminal" evidence="19">
        <location>
            <begin position="319"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00059328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00067965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CAZ81966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="408" mass="44162" checksum="16ED00CF65ACDC4C" modified="2010-06-15" version="1">MQGVAQERANAQIVQLIPFDSARKCMGVVVRLPGGVYRLYVKGASEILLNKATRYISDVTNEGPSDMELTCEGVKFLTDTINFYAERSLRTIGLLYKDYSQWPPIGAKVIEDDASMASFDDIFNEMTFVGIVGIQDPLRPGVSEAVKQCQGAGVIVRMVTGDNVITAQAIAAECGIFTEGGIIMEGPSFRKLTEAEMDDILPRLQVLARSSPEDKRILVRRLKELGETVAVTGDGTNDGPALKMADIGFSMGIAGTEVAKEASSIILMDDNFSSIVKAMMWGRAVNDAVRKFLQFQLTVNITAVLLTFVSAVSSADGSSVLTAVQLLWVNLIMDTFAALALATDAPTPQILNRKPTPKSAGLISLNMWKMIIGQAIFQLLVTYTLYFGGMTILGYSTENDEKPPQGTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5GC55</accession>
    <name>D5GC55_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Kinetochore protein NDC80</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">GSTUM_00000570001</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the NDC80 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Chromosome</location>
            <location evidence="11">Centromere</location>
            <location evidence="11">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the NDC80/HEC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430100">
        <property type="protein sequence ID" value="CAZ82098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837907.1">
        <property type="nucleotide sequence ID" value="XM_002837861.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GC55"/>
    <dbReference type="FunCoup" id="D5GC55">
        <property type="interactions" value="258"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GC55"/>
    <dbReference type="GeneID" id="9181367"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000570001"/>
    <dbReference type="eggNOG" id="KOG0995">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012583_1_0_1"/>
    <dbReference type="InParanoid" id="D5GC55"/>
    <dbReference type="OMA" id="PSHKFQK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031262">
        <property type="term" value="C:Ndc80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051315">
        <property type="term" value="P:attachment of mitotic spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.30:FF:000001">
        <property type="entry name" value="Probable kinetochore protein ndc80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.30">
        <property type="entry name" value="Ncd80 complex, Ncd80 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005550">
        <property type="entry name" value="Kinetochore_Ndc80"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055260">
        <property type="entry name" value="Ndc80_CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057091">
        <property type="entry name" value="NDC80_loop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038273">
        <property type="entry name" value="Ndc80_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10643">
        <property type="entry name" value="KINETOCHORE PROTEIN NDC80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10643:SF2">
        <property type="entry name" value="KINETOCHORE PROTEIN NDC80 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03801">
        <property type="entry name" value="Ndc80_HEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24487">
        <property type="entry name" value="NDC80_loop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 11" id="KW-0132">Cell division</keyword>
    <keyword evidence="10 11" id="KW-0137">Centromere</keyword>
    <keyword evidence="3 11" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 11" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="5 11" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinetochore protein Ndc80 CH" evidence="14">
        <location>
            <begin position="67"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="Kinetochore protein NDC80 loop region" evidence="15">
        <location>
            <begin position="398"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="270"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="329"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="530"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="15"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF03801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF24487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAZ82098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="650" mass="73909" checksum="E0B2B80DFBD9EEE3" modified="2010-06-15" version="1">MYNSGIPQPASALKRSNSNSRPSNSRMSLAARPAYNQPDVVSTAKRDPSRYSARKSYAPSSQPPPSQRRSSVMRRSSMGVAAFSQMNSFFSSAASATVQRDPRPLKDSAYRQEIASKVFEYVVNNGFELEMKHTLGPNSLKSPTQKDFSMVFQWLYKRLDPNYNFQKAIENEVLPILKTLRYPYASTITKSQLAAVGSMNSWPQFLGILHWMMELAVTMERFQSGEFDDAAAAEGVDIAAEKIIFRYLTRCYSAWLVENDDHDEFLEEMAASFEDRQSGYKEELEMLESENEQLKKQLEGMDESADPLKKLEDTRILLEGDKQKFVNYVERLELRIPKVTASNQKLKEEIEVVERELAELTAEKQSLQEAVDKQGLTPADIDRMNNDREKLSKSLSLISSKITEARIQLAERESEASSKLEALERSVSRYNSLAYQIGIIPSSSRNAEGKDFELLILPAQTPENKLLADEVGYQPAQLLNRDLRHDIKPALNRLRQEIGARIHEAQDEAIKTQELIDRIVETLADKRDEVDTLHAKVRTAVDEYQELKETTAAEGNASNAEIEKLEKELASMRLNMSNGVLALEQRMQSVTIERDQLEHACHALREKFHGEVDKMLHEVIKFKVHIQESLENYENLVEEELAGNNKLLDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5GC69</accession>
    <name>D5GC69_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Serine/threonine-protein phosphatase 2A activator</fullName>
            <ecNumber evidence="10">5.2.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Phosphotyrosyl phosphatase activator</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00000607001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Acts as a regulatory subunit for PP2A-like phosphatases modulating their activity or substrate specificity, probably by inducing a conformational change in the catalytic subunit, a direct target of the PPIase. Can reactivate inactive phosphatase PP2A-phosphatase methylesterase complexes (PP2Ai) in presence of ATP and Mg(2+) by dissociating the inactive form from the complex.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the PTPA-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="10"/>
    <dbReference type="EMBL" id="FN430103">
        <property type="protein sequence ID" value="CAZ82112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837921.1">
        <property type="nucleotide sequence ID" value="XM_002837875.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GC69"/>
    <dbReference type="FunCoup" id="D5GC69">
        <property type="interactions" value="86"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GC69"/>
    <dbReference type="GeneID" id="9184788"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000607001"/>
    <dbReference type="eggNOG" id="KOG2867">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030733_2_0_1"/>
    <dbReference type="InParanoid" id="D5GC69"/>
    <dbReference type="OMA" id="KCINTAV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008160">
        <property type="term" value="F:protein tyrosine phosphatase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04087">
        <property type="entry name" value="PTPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1150:FF:000003">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004327">
        <property type="entry name" value="Phstyr_phstse_ac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043170">
        <property type="entry name" value="PTPA_C_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037218">
        <property type="entry name" value="PTPA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10012">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10012:SF3">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 2A ACTIVATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03095">
        <property type="entry name" value="PTPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140984">
        <property type="entry name" value="PTPA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0697">Rotamase</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="376"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="392"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="418"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ82112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="554" mass="58955" checksum="319FE42ECCFB748C" modified="2010-06-15" version="1">MTSDSDLQPIDPAKHVFAIPAKRIHVQEDVPHFITSPAYKLITNFILHLNASASPLNPVTGELRNPQNYTLTSPTIHVSPEVDGIIQLIHKLEALVTRAPPDPGPRRFGNTAYRHWYSLVEDALGGPDGLLDQHLPASVLSPKGKGEVTAKQELLPYLLGSFGSSQRLDYGTGHELSFLAFLCGIWVLGGFRAGRDELGLALKCLDTYLHLVRRLVTTYTLEPAGSHGVWGLDDHSFLPYIFGSAELTTHHPTDSSSNSTVPANVEGLEDVPKPGDVVKSLVVERERLRNLYFGAIGFIYDVKKGPFWEHSPILYDISGVQGGWGKINKGMIKMFNAEVLGKFPVVQHFRFGSIFSWGGAGGSANATVITVRTQAPHASGPETGTGSGVGSGSSASSGPGTRAPENPITGTAAPWARNPTQSPTIGSNTDFTARAPWGIPASTANQTGSTAGRFPAESSASRTAPRPLPGREPGIEDARAPWATGEADKHPPSPGITQATEANLGPRRTSSPDSKLATMEGEEGRLRRGSVGTGGVRVAETGDLKKKLPPLPQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCB1</accession>
    <name>D5GCB1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">2-oxoglutarate dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="5">1.2.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">2-oxoglutarate dehydrogenase complex component E1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">GSTUM_00000661001</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + 2-oxoglutarate + H(+) = N(6)-[(R)-S(8)-succinyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:12188"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:20092"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83120"/>
            <dbReference type="EC" id="1.2.4.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the alpha-ketoglutarate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.2" evidence="5"/>
    <dbReference type="EMBL" id="FN430108">
        <property type="protein sequence ID" value="CAZ82154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837963.1">
        <property type="nucleotide sequence ID" value="XM_002837917.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCB1"/>
    <dbReference type="FunCoup" id="D5GCB1">
        <property type="interactions" value="555"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GCB1"/>
    <dbReference type="GeneID" id="9184832"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000661001"/>
    <dbReference type="eggNOG" id="KOG0450">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004709_1_0_1"/>
    <dbReference type="InParanoid" id="D5GCB1"/>
    <dbReference type="OMA" id="RDSYCRT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045252">
        <property type="term" value="C:oxoglutarate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004591">
        <property type="term" value="F:oxoglutarate dehydrogenase (succinyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006103">
        <property type="term" value="P:2-oxoglutarate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02016">
        <property type="entry name" value="TPP_E1_OGDC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1150:FF:000002">
        <property type="entry name" value="2-oxoglutarate dehydrogenase E1 component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11610:FF:000009">
        <property type="entry name" value="2-oxoglutarate dehydrogenase E1 component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12470:FF:000003">
        <property type="entry name" value="2-oxoglutarate dehydrogenase E1 component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000002">
        <property type="entry name" value="2-oxoglutarate dehydrogenase, E1 component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11610">
        <property type="entry name" value="Multifunctional 2-oxoglutarate metabolism enzyme, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1150">
        <property type="entry name" value="TPP helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032106">
        <property type="entry name" value="2-oxogl_dehyd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011603">
        <property type="entry name" value="2oxoglutarate_DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042179">
        <property type="entry name" value="KGD_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031717">
        <property type="entry name" value="ODO-1/KGD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00239">
        <property type="entry name" value="2oxo_dh_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006914">
        <property type="entry name" value="PRK09404.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008907">
        <property type="entry name" value="PRK12270.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23152:SF4">
        <property type="entry name" value="2-OXOADIPATE DEHYDROGENASE COMPLEX COMPONENT E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23152">
        <property type="entry name" value="2-OXOGLUTARATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16078">
        <property type="entry name" value="2-oxogl_dehyd_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16870">
        <property type="entry name" value="OxoGdeHyase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000157">
        <property type="entry name" value="Oxoglu_dh_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="17">
        <location>
            <begin position="676"/>
            <end position="886"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00040267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00042984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00051911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAZ82154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1047" mass="117470" checksum="3A591BADDC1FA16D" modified="2010-06-15" version="1">MLRSTLRSSCRYSGPSLALGPSRTLTTASLAERFAPRITATRRPGSLTTHKKCLDLVSRNSSTDASTRGVDPSDSFLQGNTANYIDEMYLSWKKDPSSVHISWQVYFRNMEGNGLPSQAFQAPPTLVPTPTGGVPSLVPGAHLGGNTDVTKHLKVQLLVRAYQVRGHHKANIDPLGIRSNADLGGTSQPRELDLDHYQFTEKDLDDEYTLGPGILPRFAVDGKEKMTLREIIAACEKTYCGSYGIEYVHIADRDQCDWIRERVEIPTPWEYDNHEKRRILDRLIWSSSFENFLSSKYPNDKRFGLEGCEALVPGMKALIDRSVDRGVKDIVIGMPHRGRLNVLSNVVRKPNESIFSEFGGTAEPSDEGSGDVKYHLGMNFERPTPSGKRVQLSLVANPSHLEAEDPVVLGKARAIQHFNNDEKSHNSAMGVLVHGDAAFAAQGVVYETMGFHALPAYSTGGTIHLIVNNQIGFTTDPRFARSTPYCSDIAKSIDAPIFHVNADDVEAVNFVCELASDWRAEFKRDVVIDLVCYRKYGHNETDQPSFTQPLMYRKIAEKQPALDRYISRLKAEGTFTESDIQEHKDWVWSMLEDSFAKSKDYQPTAREWLASAWNGFKTPKELSTEILPHLPTALEESQLKHIGNVIGSVPEGFNVHKNLSRILSNRQKVVNEGSGIDWSTAEALAFGSLLMEGQHVRVSGQDVERGTFSQRHAVLHDQENENTYTPLQHLSDTQAKFVISNSSLSEFGVLGFEYGYSLSSPDALVIWEAQFGDFANNAQCIIDQFIASGESKWLQRTGIVMSLPHGYDGQGPEHSSGRMERYLQLCNEDPREFPDPEKLDRQHQDCNMQVTYMTSPSNLFHVLRRQMKRQFRKPLIIFFSKSLLRHPIARSELSEFTGDSHFRWLIPETDHGKGIKDPEECKRLLFCSGQVYAALMKQRAASGIDDVAIARIEQLHPFPWAQIRNELEKYPNLDEIIWTQEEPLNAGAWTFVQPRLETILRQTDNHSAKHVKYAGRAPSASVATGMKSTHLKEEQALVNDALGISPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCE6</accession>
    <name>D5GCE6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00005854001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AVL9 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430109">
        <property type="protein sequence ID" value="CAZ82189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837998.1">
        <property type="nucleotide sequence ID" value="XM_002837952.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCE6"/>
    <dbReference type="FunCoup" id="D5GCE6">
        <property type="interactions" value="230"/>
    </dbReference>
    <dbReference type="GeneID" id="9186371"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005854001"/>
    <dbReference type="eggNOG" id="KOG3823">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009066_0_1_1"/>
    <dbReference type="InParanoid" id="D5GCE6"/>
    <dbReference type="OMA" id="IRTQFRV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018307">
        <property type="entry name" value="ABL9/DENND6_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051731">
        <property type="entry name" value="DENND11/AVL9_GEFs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037516">
        <property type="entry name" value="Tripartite_DENN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31017:SF1">
        <property type="entry name" value="LATE SECRETORY PATHWAY PROTEIN AVL9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31017">
        <property type="entry name" value="LATE SECRETORY PATHWAY PROTEIN AVL9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09794">
        <property type="entry name" value="Avl9"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50211">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDENN" evidence="3">
        <location>
            <begin position="8"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="475"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="540"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="625"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="500"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="554"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="593"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="656"/>
            <end position="680"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ82189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="680" mass="75710" checksum="9564ED5028BE8A8C" modified="2010-06-15" version="1">MDKPQRSPVVTIVGFHHARGPEVETWIGLEKEDDISDWPLLPFLALADGAHASEEDFSYFTLKHTTPAGPTTLFGISCTRQLDASELMDRPADVTRSTSYLLLLSLETTAIGCHHSLVCAEVWARILTDRSRDQFVGLSLREFVYEFKHQTLVLFKCALLQPKMLFFGSSCERLCMTQFSLLSLIPGLLRNLEDCGDPDMNNYESKLKKPTSVKTSDRKSLLTFMGLPLQIFGRGSLFGPYTPLQQLDVLADFGTKSYIVGSTNSLLLQQRDRYSDILINLDDKNINVTSSSLRAALSLSAADRRWIDVLVQSVSESWDESDPTRPKTMGFAGSEDYIRLQFEEYILSMVSSVKYHLFLEKHAGSEQNVFLPDIDGDPANDFQMDWVDAWKKTENFRIFQKFTDSELFDLVPPKHVMAGGLTMEDVQRRILQQMQELRLEEKVQNSKEVLAKTLANGRVNVSTVFNNLSKNVEKFREQRKSQSQDSMQKLSIETGEKPSTDSNPSMTIPSTVVSPVLADASSRAGAYFSSWATWAGEKRKKFAQTPSSPSSEPVKGDGSDRSLPRPPLADPPKNSFEEAIFDSRSRAVYAPSSGRSSPQRASVSGSAAPANVVIKDLDKGRGIGLGIVDAGLQSPPAPPNPSAEEKDELELTSPDPRFRPCGRREEESAPCRGEEEWACF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCG1</accession>
    <name>D5GCG1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Lysophospholipase</fullName>
            <ecNumber evidence="6">3.1.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00005876001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 1-acyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15177"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.5"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the lysophospholipase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.5" evidence="6"/>
    <dbReference type="EMBL" id="FN430109">
        <property type="protein sequence ID" value="CAZ82204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838013.1">
        <property type="nucleotide sequence ID" value="XM_002837967.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCG1"/>
    <dbReference type="STRING" id="656061.D5GCG1"/>
    <dbReference type="GeneID" id="9186386"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005876001"/>
    <dbReference type="eggNOG" id="KOG1325">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013227_1_0_1"/>
    <dbReference type="InParanoid" id="D5GCG1"/>
    <dbReference type="OMA" id="WALGRRF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002642">
        <property type="entry name" value="LysoPLipase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728">
        <property type="entry name" value="CYTOSOLIC PHOSPHOLIPASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728:SF40">
        <property type="entry name" value="PATATIN FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01735">
        <property type="entry name" value="PLA2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00022">
        <property type="entry name" value="PLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51210">
        <property type="entry name" value="PLA2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 5 6" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="4 5 6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="domain" description="PLA2c" evidence="9">
        <location>
            <begin position="200"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="37"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="626"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="798"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="629"/>
            <end position="678"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ82204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="822" mass="92204" checksum="B81FEF9488D7B48A" modified="2010-06-15" version="1">MYNTARVGLGALVTTGAVVMFYRTQIHRRILPVNERVTPGNAAVGSNGKQKGDNDETENEKAEPDESDGKAKKGILSAIKGTFGQLVVQQTPKKNPEPTPDDDLQKKSIGVVQAREEGQLNQRDALTDTQWLTFSNKLLVAKESILSIDWPKLNNLTNVFPDWATGLPEWVTKLQRELNMEPGSLADEIWSEAQDPKVNPEVEKDAHVRLGKDLCEEEQRYLASRKGFTKQGLAQYLNIPESDIHDDDVPIIATTGSGGGLRAMVAGAGYYQALTEAGLYDCTTYTAGVSGSCWLQALYLTSLGKCSFDRVMEHLKARINVHIAYPPKALELVTSAPTNKYLLRGVVERLKTGYSPFTLVDLYGLLLGARLLVPSNELTLDDTDLKLSAQRRFLENGEQPLPLYTAVRHEIPFIGGENEKGKPIPEELKEKASKHESWFQWFEATPYEIYCEELEAGIPTWAMGRRFENGVNVSRKVPEMKLPLLFGIFGSAFCATLSHYYHEIRPFVTSVSLFLSLDKMVLDRNNDLSKVHPIDPCSVPNFVKGLKESLPSTCPVLVSTQLLTPLKDAGMSNNLACYPLLRPGRNIDILIAFDSSAEIQTANWIGYAEGYAKQRKVQGWPVAIGWPKGDNKQRQQELEDAQAHSAKEAQQKLEEAQREASEAENDKPLSQRKEEQKKKNALGYCTVWVGSKEERDSGDEPPPSKAVEDDWELMKPNAGITVVYFPLIPNEKVPGVDPEISPYLSTWNFEWTPEQIDQTVALAKANFREGAEKLKGTVRAVYERKKKLRLGMEEKMIEKREKKEEERHEENRRTYGIHDHFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCQ4</accession>
    <name>D5GCQ4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Leukotriene A(4) hydrolase</fullName>
            <shortName evidence="14">LTA-4 hydrolase</shortName>
            <ecNumber evidence="14">3.3.2.10</ecNumber>
            <ecNumber evidence="14">3.4.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">GSTUM_00005966001</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>an epoxide + H2O = an ethanediol</text>
            <dbReference type="Rhea" id="RHEA:19037"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32955"/>
            <dbReference type="ChEBI" id="CHEBI:140594"/>
            <dbReference type="EC" id="3.3.2.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13 14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="13 14">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EC" id="3.3.2.10" evidence="14"/>
    <dbReference type="EC" id="3.4.11.-" evidence="14"/>
    <dbReference type="EMBL" id="FN430120">
        <property type="protein sequence ID" value="CAZ82297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838106.1">
        <property type="nucleotide sequence ID" value="XM_002838060.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCQ4"/>
    <dbReference type="FunCoup" id="D5GCQ4">
        <property type="interactions" value="959"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GCQ4"/>
    <dbReference type="MEROPS" id="M01.034"/>
    <dbReference type="GeneID" id="9187916"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005966001"/>
    <dbReference type="eggNOG" id="KOG1047">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014505_1_1_1"/>
    <dbReference type="InParanoid" id="D5GCQ4"/>
    <dbReference type="OMA" id="CTALQWM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000328">
        <property type="term" value="C:fungal-type vacuole lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061957">
        <property type="term" value="C:NVT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004301">
        <property type="term" value="F:epoxide hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120113">
        <property type="term" value="P:cytoplasm to vacuole targeting by the NVT pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09599">
        <property type="entry name" value="M1_LTA4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000009">
        <property type="entry name" value="Leukotriene A(4) hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.320:FF:000001">
        <property type="entry name" value="Leukotriene A(4) hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1730:FF:000004">
        <property type="entry name" value="Leukotriene A(4) hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2010.30:FF:000001">
        <property type="entry name" value="Leukotriene A(4) hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2010.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.320">
        <property type="entry name" value="Peptidase M1, leukotriene A4 hydrolase/aminopeptidase C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012777">
        <property type="entry name" value="LTA4H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049980">
        <property type="entry name" value="LTA4H_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038502">
        <property type="entry name" value="M1_LTA-4_hydro/amino_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034015">
        <property type="entry name" value="M1_LTA4H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015211">
        <property type="entry name" value="Peptidase_M1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02411">
        <property type="entry name" value="leuko_A4_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45726">
        <property type="entry name" value="LEUKOTRIENE A-4 HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45726:SF3">
        <property type="entry name" value="LEUKOTRIENE A-4 HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09127">
        <property type="entry name" value="Leuk-A4-hydro_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01263">
        <property type="entry name" value="Leuk-A4-hydro_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 14" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 14" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13 14" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M1 leukotriene A4 hydrolase/aminopeptidase C-terminal" evidence="15">
        <location>
            <begin position="468"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="300"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="387"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="134"/>
            <end position="136"/>
        </location>
        <ligand>
            <name>a peptide</name>
            <dbReference type="ChEBI" id="CHEBI:60466"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="270"/>
            <end position="275"/>
        </location>
        <ligand>
            <name>a peptide</name>
            <dbReference type="ChEBI" id="CHEBI:60466"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="322"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="570"/>
            <end position="572"/>
        </location>
        <ligand>
            <name>a peptide</name>
            <dbReference type="ChEBI" id="CHEBI:60466"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR612777-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR612777-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR612777-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM01263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAZ82297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="615" mass="69242" checksum="F6CE1487E2E5E7C8" modified="2010-06-15" version="1">MAPTRDPNTLSNHLDFSTTHTAISLTLSFSKSSLYGEVELTLKALADGLKQVVLDTSFLDVRAVAVNGKGAKWSIGDRVEPYGSPLKVDLEKAVNSQDEVIIKVTWGTTEKCTALQWMTPEQTSNKRHPYMFSQCQAIHARSLYPCQDTPSVKSTFDFSIRSPLPVCASGLPTGVHHFVPATSPSEPGTLAYTFRQPIPIPSYLMAIASGDIANAAVGPRSLVYAGPEELLRCKKELEDDVETFIKTAESLIYAYEWTTYNVLILPPSFPYGGMENPCITFATPTIISGDKSNVDVIAHELSHSWSGNLVSNASWEHFWLNEGWTTYLERRIQGKVHGDKHFHFSAIIGWKALKGSVELYGPDHEFTKLVIDLKGKDPDDAFSSIPYEKGFNFLYYLDRLVGREKWDTFIPHYFSTYRKKSLTSFDFRETLLSFFSKDLEASDALAKVDWDTWFYKPGLPEKPDFDTTLADACYALADRWQALNSSDSSFKPSPSDISCWTSGQVVVFLERITDFSTPLRKELVELMRGEYGFLKSENAEILSRFLTVGLKAKDERLYSKAAVVLGMWGRMKFVRPLYRLLNECDRNLALETFEKNKGFYHPICRGVVMKDLGIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCR8</accession>
    <name>D5GCR8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00005991001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430120">
        <property type="protein sequence ID" value="CAZ82311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838120.1">
        <property type="nucleotide sequence ID" value="XM_002838074.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCR8"/>
    <dbReference type="STRING" id="656061.D5GCR8"/>
    <dbReference type="GeneID" id="9187930"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005991001"/>
    <dbReference type="HOGENOM" id="CLU_2086550_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCR8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="117" mass="12667" checksum="446ABEBBDF022768" modified="2010-06-15" version="1">MDQAKPPPTISTSASTSCPGSGSLISIQRFLIAYPDREGHRAANQKSGLLLAWGQAEVWYYTGTPLGPSRCDKTLLSKAAANESRANFISICGPELLGVCLAHHDRELFVISNHGWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCZ1</accession>
    <name>D5GCZ1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00000884001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430130">
        <property type="protein sequence ID" value="CAZ82384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838193.1">
        <property type="nucleotide sequence ID" value="XM_002838147.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCZ1"/>
    <dbReference type="FunCoup" id="D5GCZ1">
        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GCZ1"/>
    <dbReference type="ESTHER" id="tubmm-d5gcz1">
        <property type="family name" value="Acidic_Lipase"/>
    </dbReference>
    <dbReference type="GeneID" id="9183846"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000884001"/>
    <dbReference type="eggNOG" id="KOG2624">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024238_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCZ1"/>
    <dbReference type="OMA" id="AYCTNDD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000193">
        <property type="entry name" value="Ab-hydrolase associated lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006693">
        <property type="entry name" value="AB_hydrolase_lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11005">
        <property type="entry name" value="LYSOSOMAL ACID LIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04083">
        <property type="entry name" value="Abhydro_lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="160"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="466"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="Partial AB-hydrolase lipase" evidence="3">
        <location>
            <begin position="262"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="220"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="557"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="566"/>
            <end position="588"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ82384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="694" mass="78133" checksum="9DF31FFAC3D43465" modified="2010-06-15" version="1">MLSPNSLEGSSSSASLDNASASLGTSDLLDHASSAYRAIEITSTSTVLETARGHRLPGRLIEEARKLNALRSEEEGKRGRDGNLSPPLGVKGQRRTLSPGLRDTARNRLQKTGGHLDTMEERDGGGENVYIGKATAFTNPLFPPLPVYGPPTPLRRLQYMIFRACSGVLSFCFLFIIVIGAIAKSLPSMFMTLACLLRGRDPNATRPFYGIEKERAEKRREETRAWKARRRDSLGAGDQDGERGLRNKQITGGEDNLVCDIGYYARRVGLDAEEFEVQTEDGFLIMMQHIYDPDDPPYYPSEKIDGKDNRGRSANGKRSKYPVLLMHGLLQSSGAFCVNDEDSLAFYLCKAGYDVWLGNNRCGFKPKHTSLHYSDPRMWAWNIRQMGINDLPALIDHVLSVTRFPKLGLICHSQGTTQTFVALAKQQRPDIGSKISVFCALAPAAYAGPLIDKFCFKFMKIISPSMFRIVFGIHAFIPFMMLMHATMPARVYGALGYRVFWFLFGWSDTRWDKGLRDRFFQFAPVFVSAEAMRWWLGRECFARHKCILSTKREEEEADQEEEGILEQEKQKEPEKERNEGSKGPDKELSPSPSTNGGPWYDGRCPPMALWVAGNDNLVDGPRLIRRFERGKEPDVNLVHAKVIEEYEHLDVVWAVDAPEQVFKELVDVVWKCVDEDVKREVIVPRGIGESGIRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDA7</accession>
    <name>D5GDA7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00006126001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430142">
        <property type="protein sequence ID" value="CAZ82500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838309.1">
        <property type="nucleotide sequence ID" value="XM_002838263.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDA7"/>
    <dbReference type="FunCoup" id="D5GDA7">
        <property type="interactions" value="384"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GDA7"/>
    <dbReference type="GeneID" id="9183964"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006126001"/>
    <dbReference type="eggNOG" id="KOG2355">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057592_3_0_1"/>
    <dbReference type="InParanoid" id="D5GDA7"/>
    <dbReference type="OMA" id="YLGTEWV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030014">
        <property type="term" value="C:CCR4-NOT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00267">
        <property type="entry name" value="ABC_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001332">
        <property type="entry name" value="Similar to ABC transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43158">
        <property type="entry name" value="SKFA PEPTIDE EXPORT ATP-BINDING PROTEIN SKFE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43158:SF2">
        <property type="entry name" value="SKFA PEPTIDE EXPORT ATP-BINDING PROTEIN SKFE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="8"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="261"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ82500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="280" mass="30912" checksum="65D1035A4A2B994E" modified="2010-06-15" version="1">MAHHPPSIDIQNLTYTFPDNSIGLEDININLPAGSRTLLIGANGAGKTTLLRLASGKKLTTRGRIRIGGVDPFAHGLEGVTYLGLEWVLNPIVRNDIYVHELLASVGGDAYPERRDQLVRILDIDVDWRMHIASDGERRRVQLAMGLLRPWSILLIDEVTVDLDVLARFRFLEFLKEETETRGCTIVYATHIMDGIAGWPTNLVRMAGGKVKAVGTVASFLGEVERKGASAGCRDGGIMRNSALLDLALGWLVEDLEERGPRDRTKSGQVTYNNVEKEPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDL9</accession>
    <name>D5GDL9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GTP cyclohydrolase 1</fullName>
            <ecNumber evidence="3">3.5.4.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">GTP cyclohydrolase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00001086001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">GTP cyclohydrolase 1 is the first enzyme in the biosynthetic pathway leading to folic acid.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; 7,8-dihydroneopterin triphosphate biosynthesis; 7,8-dihydroneopterin triphosphate from GTP: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GTP cyclohydrolase I family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.16" evidence="3"/>
    <dbReference type="EMBL" id="FN430150">
        <property type="protein sequence ID" value="CAZ82612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838421.1">
        <property type="nucleotide sequence ID" value="XM_002838375.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDL9"/>
    <dbReference type="FunCoup" id="D5GDL9">
        <property type="interactions" value="333"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GDL9"/>
    <dbReference type="GeneID" id="9181616"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001086001"/>
    <dbReference type="eggNOG" id="KOG2698">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049768_2_0_1"/>
    <dbReference type="InParanoid" id="D5GDL9"/>
    <dbReference type="OMA" id="HFACRPQ"/>
    <dbReference type="UniPathway" id="UPA00848">
        <property type="reaction ID" value="UER00151"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003934">
        <property type="term" value="F:GTP cyclohydrolase I activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00642">
        <property type="entry name" value="GTP_cyclohydro1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.286.10:FF:000003">
        <property type="entry name" value="GTP cyclohydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1130.10:FF:000012">
        <property type="entry name" value="GTP cyclohydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.286.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00223">
        <property type="entry name" value="FolE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043133">
        <property type="entry name" value="GTP-CH-I_C/QueF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043134">
        <property type="entry name" value="GTP-CH-I_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001474">
        <property type="entry name" value="GTP_CycHdrlase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018234">
        <property type="entry name" value="GTP_CycHdrlase_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020602">
        <property type="entry name" value="GTP_CycHdrlase_I_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00063">
        <property type="entry name" value="folE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006825">
        <property type="entry name" value="PRK09347.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006826">
        <property type="entry name" value="PRK09347.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11109:SF7">
        <property type="entry name" value="GTP CYCLOHYDROLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11109">
        <property type="entry name" value="GTP CYCLOHYDROLASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01227">
        <property type="entry name" value="GTP_cyclohydroI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55620">
        <property type="entry name" value="Tetrahydrobiopterin biosynthesis enzymes-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00859">
        <property type="entry name" value="GTP_CYCLOHYDROL_1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00860">
        <property type="entry name" value="GTP_CYCLOHYDROL_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="8" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GTP cyclohydrolase I" evidence="12">
        <location>
            <begin position="99"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00055676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ82612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="279" mass="31627" checksum="B6FEAD31AA6CD69E" modified="2010-06-15" version="1">MPPPINTDQLTNQIDHRVFRIRENGGPANDIVPTESTTNQRRRTSIALALGDAHPRAELEASRRQFVDYDGLSWPSSGTRERLEATPAEAEARLEKLSGAIRTILECIGEDPDREGLLETPSRYAKAMLYFTKGYEENLKLIINNAVFREDHDEMVIVKDIEIYSMCEHHLVPFTGKMHIGYIPRGKVLGLSKFARIAEMFARRLQLQERLTKQVAVAIEEILEPLGVAVVMESAHLCMVMRGIEKTTATTTTSSMLGCFRKRTRTREEFLRLALNSTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDS4</accession>
    <name>D5GDS4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00006249001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430153">
        <property type="protein sequence ID" value="CAZ82667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838476.1">
        <property type="nucleotide sequence ID" value="XM_002838430.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDS4"/>
    <dbReference type="STRING" id="656061.D5GDS4"/>
    <dbReference type="GeneID" id="9184462"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006249001"/>
    <dbReference type="eggNOG" id="KOG1852">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3351">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_356859_0_0_1"/>
    <dbReference type="InParanoid" id="D5GDS4"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.30">
        <property type="entry name" value="MOB kinase activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005301">
        <property type="entry name" value="MOB_kinase_act_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036703">
        <property type="entry name" value="MOB_kinase_act_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22599">
        <property type="entry name" value="MPS ONE BINDER KINASE ACTIVATOR-LIKE MOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03637">
        <property type="entry name" value="Mob1_phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01388">
        <property type="entry name" value="Mob1_phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101152">
        <property type="entry name" value="Mob1/phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="258"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="26"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="48"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="275"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="285"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="298"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="356"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="366"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR605301-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ82667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="786" mass="86516" checksum="5731CC0A6766330D" modified="2010-06-15" version="1">MGPSSNPLSSPPRLPSPPPFTEVQISPVPLSLSLSKSPVRPVSNSATRRIRPGTKSRHIAAGPPLVPLSELESAFQLQEHLASLLAATTSPPGSDTTVPLSREACERLATPPEGIEEYLWCYELTRRLTRDLNILLVALLKDNCTASSCPEMRASEWQYLCAVHDPPQSCCAIDYSTHTLDQAATMLCTNRYFPSRLSPHNTSVKHLASIFRRLYRIFAHAWFQHRSVFWEVENEFGLYLFFKTVSEKYSLIPEDNLTLPPEAEGLKSKNSFGEVDSDEEEIDEFDRGAGSREHGQELDEDEDYPDSDESESDEEEADGEEEGELEIENSIEGDEVEDDEFDLDIDDDHSVEEEGSGNSALTETHGNFKGETRGGEREHNLLLLPSPQSYSLVSLMETYKLAFGAAFRAAPLDTPSTLDIAVYAPFLSSLNAVRGRHFDKTQHLLARLYSIVNSVAVEEKRELAVDARIILLSDIGPKEEERASGFGPVIGLRELAKSGREWKNIIVGAHSPGSSEGQENREKEEAEGQAIEKAFLCYRGDKVTGYTDVWRCEIVTLSEEEPKEAVENDKLDDAAVEHLIVAGMPSTSTTSLHPFADPYKVGGTFDHLHPGHKLLLTMTAFLLSPNAAAPRLVAGITGETLLINKKHSAHLGSWDSRAAEVVDFLRGITDFSANPSQLLTSPSSSTPETDITPRSITATIPGGEKELVIETVEINDPFGPTITNEAISALVVSEETKNGGEAVNKKRTEKSWDALQVYKVDVIDNEGGVKMSSTDIRRKLEERSTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDU4</accession>
    <name>D5GDU4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pyrroline-5-carboxylate reductase</fullName>
            <ecNumber evidence="5">1.5.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00006277001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-proline + NADP(+) = (S)-1-pyrroline-5-carboxylate + NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:14109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17388"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="EC" id="1.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis; L-proline biosynthesis; L-proline from L-glutamate 5-semialdehyde: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the pyrroline-5-carboxylate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.2" evidence="5"/>
    <dbReference type="EMBL" id="FN430153">
        <property type="protein sequence ID" value="CAZ82687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838496.1">
        <property type="nucleotide sequence ID" value="XM_002838450.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDU4"/>
    <dbReference type="FunCoup" id="D5GDU4">
        <property type="interactions" value="415"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GDU4"/>
    <dbReference type="GeneID" id="9184482"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006277001"/>
    <dbReference type="eggNOG" id="KOG3124">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042344_1_1_1"/>
    <dbReference type="InParanoid" id="D5GDU4"/>
    <dbReference type="OMA" id="VWAVKPQ"/>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00361"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004735">
        <property type="term" value="F:pyrroline-5-carboxylate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3730.10:FF:000001">
        <property type="entry name" value="Pyrroline-5-carboxylate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3730.10">
        <property type="entry name" value="ProC C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01925">
        <property type="entry name" value="P5C_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028939">
        <property type="entry name" value="P5C_Rdtase_cat_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053790">
        <property type="entry name" value="P5CR-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029036">
        <property type="entry name" value="P5CR_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000304">
        <property type="entry name" value="Pyrroline-COOH_reductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00112">
        <property type="entry name" value="proC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11645">
        <property type="entry name" value="PYRROLINE-5-CARBOXYLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11645:SF0">
        <property type="entry name" value="PYRROLINE-5-CARBOXYLATE REDUCTASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03807">
        <property type="entry name" value="F420_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14748">
        <property type="entry name" value="P5CR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000193">
        <property type="entry name" value="Pyrrol-5-carb_rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00521">
        <property type="entry name" value="P5CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 4 5" id="KW-0521">NADP</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0641">Proline biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="Pyrroline-5-carboxylate reductase catalytic N-terminal" evidence="7">
        <location>
            <begin position="72"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="Pyrroline-5-carboxylate reductase dimerisation" evidence="8">
        <location>
            <begin position="214"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="13"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>NADPH</name>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000193-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF14748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ82687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="320" mass="33623" checksum="8327A915E096C039" modified="2010-06-15" version="1">MEPTPDSLTLTILGCGTMGVAILSGILSSLSTSLSTSLSRLPPSASGTSTPTGGALLPTRFHDASRTPTRLPSKFIACVKRPESASRVRRAFEGFAGAPAVEVYTGRNVEGVRGADVVILGCKPQMYAEILREEGMREALEGKLLVSILAGVKIAALRVVVPYSTRVVRAMPNTASMIRESMTVISAEDDISPEEKALVSWIFSQVGRSLVLGEKHMNACTALCGSGPAFYALIVEAMADGGVMMGLPRAEAQMMAAQTMQGAGRMVLAGHHPAIIREQVSTPAGCTTHGLLILEDGKVRSTIARTIREATNMAAGLGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5GE63</accession>
    <name>D5GE63_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Protein ROT1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00006397001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Required for normal levels of the cell wall 1,6-beta-glucan. Involved in a protein folding machinery chaperoning proteins acting in various physiological processes including cell wall synthesis and lysis of autophagic bodies.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ROT1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430164">
        <property type="protein sequence ID" value="CAZ82806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838615.1">
        <property type="nucleotide sequence ID" value="XM_002838569.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GE63"/>
    <dbReference type="FunCoup" id="D5GE63">
        <property type="interactions" value="23"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GE63"/>
    <dbReference type="GeneID" id="9183138"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006397001"/>
    <dbReference type="eggNOG" id="ENOG502QQTG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071622_0_0_1"/>
    <dbReference type="InParanoid" id="D5GE63"/>
    <dbReference type="OMA" id="YKPPQML"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006458">
        <property type="term" value="P:'de novo' protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019623">
        <property type="entry name" value="Rot1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28090">
        <property type="entry name" value="PROTEIN ROT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28090:SF1">
        <property type="entry name" value="PROTEIN ROT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10681">
        <property type="entry name" value="Rot1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017290">
        <property type="entry name" value="ROT1_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0732">Signal</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072726" description="Protein ROT1" evidence="12">
        <location>
            <begin position="23"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="230"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR017290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ82806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="248" mass="27692" checksum="0D35B9A4DBA8A697" modified="2010-06-15" version="1">MLPSLSVTPLAVTLLALPFVSAQDNVTSLTGTWSSKSNAVFTGPASDCLSLLFSFYDPVNEKFFEPTLTGTSYSFTDDGFYEEAVYIAVANPTNPSCTKGVLQWQHGGYVIAENGSIVLHPIMVDGRQLYSDPCKHSTSIYTRFNTTEVFKRWEIFVDEYRGMHRLNLYKFDGAPMNPMYLAYRPPQMLPTQTLNPTVSAPKATRASKLKVKRALPSNLKKAENINADRWWWIGVGMTGVGFLGFFLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>D5GED9</accession>
    <name>D5GED9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00006438001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430175">
        <property type="protein sequence ID" value="CAZ82882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838691.1">
        <property type="nucleotide sequence ID" value="XM_002838645.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GED9"/>
    <dbReference type="STRING" id="656061.D5GED9"/>
    <dbReference type="GeneID" id="9185538"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006438001"/>
    <dbReference type="HOGENOM" id="CLU_752694_0_0_1"/>
    <dbReference type="InParanoid" id="D5GED9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="56"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="125"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="176"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="258"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="296"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ82882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="368" mass="41854" checksum="7CAB8AB98C6E2056" modified="2010-06-15" version="1">MWYCPDFYKGFCPPLQDQSHLYGLGIRMGIYLIWLSSILAYTLLHNPISRYSSRDLNYCFSFAFFLTLTLDSQTKPRDKHYQADALVVILLLAATATLLTALEVLERTRPKAKRREEGRSRLGDFLRLCLLAGFIVYLNYWWFRGVYDIKSVCARHTHFFTKPVRILGGFRLIGQIWSPLLIVLFIPVLITALRWGNKKRRHAAMNRRRARESEGGSHPGPLKKGYWAPPPPTPLPVNGISPASSISDHEGPKKNKRALFAAWILGFGALFIILFIELMLAYSGNPGQINRVNDNAQLIPLLIGCGAIALVLSRLYEQRLKICGEENYREYQRAFLYDACAPYPFNRSAVRSAGDKFMKTRSRGSGEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEF5</accession>
    <name>D5GEF5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 12">1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase</fullName>
            <ecNumber evidence="4 12">5.3.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 12">5-proFAR isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9 12">Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00006458001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide = 5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:15469"/>
            <dbReference type="ChEBI" id="CHEBI:58435"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="5.3.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 4/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the HisA/HisF family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.16" evidence="4 12"/>
    <dbReference type="EMBL" id="FN430175">
        <property type="protein sequence ID" value="CAZ82898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838707.1">
        <property type="nucleotide sequence ID" value="XM_002838661.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEF5"/>
    <dbReference type="FunCoup" id="D5GEF5">
        <property type="interactions" value="203"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GEF5"/>
    <dbReference type="GeneID" id="9181529"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006458001"/>
    <dbReference type="eggNOG" id="KOG3055">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065050_0_0_1"/>
    <dbReference type="InParanoid" id="D5GEF5"/>
    <dbReference type="OMA" id="IEWNKTH"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00009"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003949">
        <property type="term" value="F:1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04723">
        <property type="entry name" value="HisA_HisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000110">
        <property type="entry name" value="1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011858">
        <property type="entry name" value="His6/HISN3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006062">
        <property type="entry name" value="His_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044524">
        <property type="entry name" value="Isoase_HisA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02129">
        <property type="entry name" value="hisA_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43090">
        <property type="entry name" value="1-(5-PHOSPHORIBOSYL)-5-[(5-PHOSPHORIBOSYLAMINO)METHYLIDENEAMINO] IMIDAZOLE-4-CARBOXAMIDE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43090:SF2">
        <property type="entry name" value="1-(5-PHOSPHORIBOSYL)-5-[(5-PHOSPHORIBOSYLAMINO)METHYLIDENEAMINO] IMIDAZOLE-4-CARBOXAMIDE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00977">
        <property type="entry name" value="His_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 11" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="8 12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU364022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ82898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="258" mass="28351" checksum="4DF5530944E00F36" modified="2010-06-15" version="1">MTKFRACIDIHNNLVKQIVGGTLSSASPSTLKTNHTSLHPPAHFSALYRDAQVTGSHVILLSPDSHPAATEALAAWPGGLQVGGGITADNALEWLDERGAEKVIVTSWLFPRGRVEVDRLEELVRRVGGRGRVVIDLSCRRVGGKWMVAMERWSRVTEVEVCKETLDMLSDYCSEFLIHAADVEGLCRGIDEELVERLGEWVTIPTTYAGGGKEFADLERVERLSGGKVDLTFGSALDLFGGSGVKFEDCVRWNRERS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEG3</accession>
    <name>D5GEG3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00006473001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <location evidence="6">Coated pit</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the adaptor complexes medium subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430175">
        <property type="protein sequence ID" value="CAZ82906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838715.1">
        <property type="nucleotide sequence ID" value="XM_002838669.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEG3"/>
    <dbReference type="FunCoup" id="D5GEG3">
        <property type="interactions" value="159"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GEG3"/>
    <dbReference type="GeneID" id="9181537"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006473001"/>
    <dbReference type="eggNOG" id="KOG0938">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026996_5_2_1"/>
    <dbReference type="InParanoid" id="D5GEG3"/>
    <dbReference type="OMA" id="VWKIPRI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030131">
        <property type="term" value="C:clathrin adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09251">
        <property type="entry name" value="AP-2_Mu2_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14836">
        <property type="entry name" value="AP2_Mu_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000002">
        <property type="entry name" value="AP-2 complex subunit mu, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1170">
        <property type="entry name" value="Mu homology domain, subdomain B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050431">
        <property type="entry name" value="Adaptor_comp_med_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036168">
        <property type="entry name" value="AP2_Mu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043532">
        <property type="entry name" value="AP2_Mu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001392">
        <property type="entry name" value="Clathrin_mu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028565">
        <property type="entry name" value="MHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043512">
        <property type="entry name" value="Mu2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10529">
        <property type="entry name" value="AP COMPLEX SUBUNIT MU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00928">
        <property type="entry name" value="Adap_comp_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005992">
        <property type="entry name" value="Clathrin_mu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00314">
        <property type="entry name" value="CLATHRINADPT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49447">
        <property type="entry name" value="Second domain of Mu2 adaptin subunit (ap50) of ap2 adaptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51072">
        <property type="entry name" value="MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0168">Coated pit</keyword>
    <keyword evidence="2" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MHD" evidence="8">
        <location>
            <begin position="168"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR005992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ82906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="436" mass="49178" checksum="12FE6ADB86D2AC40" modified="2010-06-15" version="1">MLSGILLFNQKGENLIFRQFRNDCRPRLSDVFRIQVISNAQVRSPILTLGSTTFSHVKHENIYLVAVTKSNANAALVFEFLYRLIALGRSYFGKFDEEAVKNNFVLIYELLDEILDFGYPQNTETDTLKMYITTEGVKTERAIEDSTRITMQATGALSWRRADVKYRKNEAFVDVIEDVNLLMSAGGTVLRADVSGQIIMRAYLSGTPECKFGLNDRLLLDGDGLTRPSGNKSGTKATRAAAGSVTLEDCQFHQCVKLGKFDTDRIISFVPPDGEFELMRYRATENVNLPFRVHAIVNEIGKTKVEYQVAIRANYGTKLFATNVVVRVPTPLNTAGIQTRTSQGKAKYEPSENHIVWKIPRFTGQAEYVLSADATLTSMTNQKAWSRPPLSLSFSLLMFTSSGLLVRYLKVFEKSNYSSVKWVRYMTRAGSYEIRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEV0</accession>
    <name>D5GEV0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00001404001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430194">
        <property type="protein sequence ID" value="CAZ83043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838852.1">
        <property type="nucleotide sequence ID" value="XM_002838806.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEV0"/>
    <dbReference type="STRING" id="656061.D5GEV0"/>
    <dbReference type="GeneID" id="9188581"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001404001"/>
    <dbReference type="eggNOG" id="ENOG502QT0Z">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008719_3_2_1"/>
    <dbReference type="InParanoid" id="D5GEV0"/>
    <dbReference type="OMA" id="REELWAY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF40">
        <property type="entry name" value="ZN(2)-C6 FUNGAL-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="60"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="535"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="620"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="62"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="74"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="87"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="126"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="553"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="564"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ83043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="643" mass="73701" checksum="3DC4FF931F33A8DB" modified="2010-06-15" version="1">MKEHLIVRTVGNPAGYVSAVYALILLTSRPSNLPWFRYHTNGRCIFRTSRGTLLLTFARVTPPKPTRNSPPTPRQTATVTTVTSAPAPHPTLPPILPLLPTPVHTGEPANNFEDVKPHHRQGYPAYAPPPPPPPPPPSSTINSDHTMSPPPPPKEVRNYLSSAEETLFMQVFVEEVGIWMDSMDPYKHFSRLLPFSSLNEPMLLNAFLACGARHLTLVNPAYHEDKALHYYDTATRLLLASLQNPNRDTEMCATTAVILNVYEIMSERALQRMNHIAGARALLKECRWSARTGGVGGACFWLNVSMELLSCLHFNWAVAWEPDEWGVDMDFNRYCEPGREELWAYRIIYVLAKIANFRAALPKQQQEAKTVTEQMYLNSETQKRCEEWKRLRDLCVAWNDHIPRSMHPMAYLHSYRTTTGSAFPEVWLVKRCAIVARLFYHTAMVLLAQMNPFMPRESDEMRNMEMTNSHLICGIAAHVKDRGVASVALRSLAIAAECLTVRREQEEVLEIFTKIKKETGWRVNFVYKELKEKWGWEAPDPPPQPSSLLQSSTMMHQSPTPSNQFAQYQRQQQQHQHQHQQQQQHQQQQHHQQQAQQQQQAQVRRLPQLVNPLMAVSDYTSNSHPYHPHYVSAPPQVERHHYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>D5GF72</accession>
    <name>D5GF72_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00006757001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430208">
        <property type="protein sequence ID" value="CAZ83165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838974.1">
        <property type="nucleotide sequence ID" value="XM_002838928.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GF72"/>
    <dbReference type="STRING" id="656061.D5GF72"/>
    <dbReference type="GeneID" id="9182349"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006757001"/>
    <dbReference type="HOGENOM" id="CLU_3034076_0_0_1"/>
    <dbReference type="InParanoid" id="D5GF72"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ83165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="55" mass="6396" checksum="0433C56B0DABBE39" modified="2010-06-15" version="1">MEGCWDRLYVHIRKVKLLKSPKFDLGALLNLHGESTADESGQKVEREFNFSYLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFA0</accession>
    <name>D5GFA0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00006795001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430208">
        <property type="protein sequence ID" value="CAZ83193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839002.1">
        <property type="nucleotide sequence ID" value="XM_002838956.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFA0"/>
    <dbReference type="STRING" id="656061.D5GFA0"/>
    <dbReference type="GeneID" id="9188049"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006795001"/>
    <dbReference type="eggNOG" id="ENOG502QWQI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023476_0_0_1"/>
    <dbReference type="InParanoid" id="D5GFA0"/>
    <dbReference type="OMA" id="LQGMHIL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38702">
        <property type="entry name" value="CALPONIN-HOMOLOGY (CH) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38702:SF1">
        <property type="entry name" value="CALPONIN-HOMOLOGY (CH) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="48"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="520" mass="57908" checksum="32696FEF1FD459B0" modified="2010-06-15" version="1">MSGDTTPILSPRSTPTKQDSQNVSPHTLPMTPPSPAFTFTPGELGYVSSDTSSDSSARSSLARTDLGGPRRRGFMRPQGTEFSTSARSRESVMALGSIAHLQYFFAKTGLLDGKGAGLQTKKSSNSLKVDTRAQGIPQDSTYSSLRSSPELFMPMEADFFSESPSELDDFAFDESVMLPPTTSTYNPQTKHIPPTPNPQVLRERLRGALAVTKSAWLQSFAIPGGPARKEKVEGEEDLAPLPPGFSELHGNELVELATSAIRAAKAYYYTTDTSLLSTLDDRSLREGFLTTLDALKRLAQRKFDGGVKPEERNAVVKWIDSVELSLAAEENAIADMRKKGREWLEGSWEGREMDRCHLFLTYFDSSPSPLPPFVPTEPATPLPTPFLLSLRSGLRLILIHNAVVRRSKRPFGQIPNYHKEFSKPYRSAENLRFWKKAAEIRWELRLKFDVTSVVNGTEDGWQGFQRDIRYWCEKVLEEIRTDWEVGDVASGGEEREEGRVRQTRSGTLESLNVEIGGYIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFW8</accession>
    <name>D5GFW8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007120001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430220">
        <property type="protein sequence ID" value="CAZ83411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839220.1">
        <property type="nucleotide sequence ID" value="XM_002839174.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFW8"/>
    <dbReference type="STRING" id="656061.D5GFW8"/>
    <dbReference type="GeneID" id="9181756"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007120001"/>
    <dbReference type="eggNOG" id="ENOG502S5ST">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032571_2_2_1"/>
    <dbReference type="InParanoid" id="D5GFW8"/>
    <dbReference type="OMA" id="PCRGHHK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.50.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36182">
        <property type="entry name" value="PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G10930)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36182:SF1">
        <property type="entry name" value="PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G10930)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072316" evidence="1">
        <location>
            <begin position="19"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="217" mass="22632" checksum="7A8E9978B66E4E33" modified="2010-06-15" version="1">MLLIKAAILSFALSAANAHMILRNPPSFGFIGNKLSTKPDVDLNAPISKAQFPCKGYHKDPDRGAGKSVATWAAGSTQSFSLAGGAPHGGGSCQVSLSYDNGETFRVLKSFIGNCPSADTGREYSFPIPRDAASGAAIFAWTWYNRIGNRELYMGCAAVTITHGGSGLNSSFPKIFVANIGEQCTTVANAVLEFPNPGAVVERSGEVALKPEGAGCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGA4</accession>
    <name>D5GGA4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00007292001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430253">
        <property type="protein sequence ID" value="CAZ83547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839356.1">
        <property type="nucleotide sequence ID" value="XM_002839310.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGA4"/>
    <dbReference type="GeneID" id="9182340"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007292001"/>
    <dbReference type="HOGENOM" id="CLU_674719_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGA4"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ83547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="408" mass="45160" checksum="C9C2F400FB876A5F" modified="2010-06-15" version="1">MTSGTYRSPGSRNRLESLDLLSILLVTESSDDVAAISIYRTAADRIELWYSKDRPCTEIEKANITKFIQSAARTAHLSKPAREDLLRIALRMCKRKVFACIYEARHLMVRFSRDGSYGIIPRASQADHDLRDAATIKLRRILGENDFPSGRSLEAFLTLLFQAIILAIRPGVPFDIAANVEFYFEAVIRCHALLQVPVIDTVIEGTLLGRLMEIADYYTAVAIAHREIQKLSPGGKAYLIRSIREIPPSSPTTYLTPPDPLTCLNHWARYRGLTLSSSSSPSSLPAFPGKTQIRECTHPEATLITKIISAHKSSPTVLRDIGSSRISCWICQEFSAAACDLYPGICIRLSREMLATAGSWKFPVDTPACLVLALTRRVERAMGMVFEGVDSLGGIGRGVKRKIGEMEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGJ1</accession>
    <name>D5GGJ1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="13">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00002041001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the epsin family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430271">
        <property type="protein sequence ID" value="CAZ83634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839443.1">
        <property type="nucleotide sequence ID" value="XM_002839397.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGJ1"/>
    <dbReference type="FunCoup" id="D5GGJ1">
        <property type="interactions" value="117"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GGJ1"/>
    <dbReference type="GeneID" id="9185557"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002041001"/>
    <dbReference type="eggNOG" id="KOG2056">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012678_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGJ1"/>
    <dbReference type="OMA" id="STEFYDI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030125">
        <property type="term" value="C:clathrin vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070530">
        <property type="term" value="F:K63-linked polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000147">
        <property type="term" value="P:actin cortical patch assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd16991">
        <property type="entry name" value="ENTH_Ent1_Ent2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ENTH"/>
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        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
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        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276:SF110">
        <property type="entry name" value="EPSIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276">
        <property type="entry name" value="EPSIN/ENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01417">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ENTH"/>
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    </dbReference>
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        <property type="entry name" value="UIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="ENTH" evidence="12">
        <location>
            <begin position="10"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="179"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="225"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="278"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="464"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="230"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="282"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="296"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="318"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="362"/>
            <end position="376"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="491"/>
            <end position="502"/>
        </location>
    </feature>
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        <location>
            <begin position="508"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010130"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ83634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="524" mass="58119" checksum="C333B49220188369" modified="2010-06-15" version="1">MSKVIRTVKNVTKGYSSTQVKVRNATSNDLWGPTGTEMSDIARMTFETTHEFFEIMDMLDKRLNDKGKNWRHVLKALKVLDYCLHEGSEHVVVWAKDNIYIIKTLREFQFIDEDGRDQGLNVRISAKELTSLIMDEDRLRNERKDRKASLLAKRWNEGTSSRQDEEDLEYRLAIEASKNQAEEDKKRSANKQPDLDDDLAKAIKLSKEEEELRQRELEQNNADSLFDDATPTQSQPQTFQPAGYQPQQYQQLDQVDFFGNPVGQAPLQQQNTSYLANVYSNPTGFPQQQFPQPTGYDQSQAQFSVSQANPYAPPPSSSPQQTSFLQPGDNNPYANGPAAQPLQPLPTGSNNPFAPKMNNFNAATVPPQQQAQAYKPPSFPNPPMQNEKPINPQHAHLESLLAAGNDNGLDTYGNVGNLRIPAQHTAPGTFVNSAGTSRTGRPLAAQSTGTNPFFNQQFTGVPHSAGFGSSLSPQPNRLLPSHTGPAGMNGYGTSSFGQQQQPNPYPTGFGQPQQQQQGGNLIDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGZ9</accession>
    <name>D5GGZ9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Signal recognition particle 54 kDa protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">GSTUM_00007635001</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Signal-recognition-particle (SRP) assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum (ER) membrane. SRP is required for the cotranslational protein translocation for ER import and preferentially recognizes strongly hydrophobic signal sequences. It is involved in targeting the nascent chain-ribosome (RNC) complex to the ER and is proposed to participate in the arrest of nascent chain elongation during membrane targeting. SRP54 binds to the signal sequence of presecretory protein when they emerge from the ribosomes. SRP54 interacts with the scR1 RNA and mediates the association of the resulting SRP-RNC complex with the signal recognition particle receptor (SR) via its alpha subunit SRP101. Both, SRP54 and SRP101, are locked in their GTP bound forms in the SRP-RNC-SR complex, which dissociates upon transferring the signal sequence to the protein-conducting channel (translocon). After signal sequence transfer, SRP54 and SRP101 act as reciprocal GTPase-activating proteins (GAPs), thereby resolving their association.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">Fungal signal recognition particle consists of a 7S RNA molecule (scR1) and at least six protein subunits: srp72, srp68, srp54, sec65, srp21 and srp14.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 13">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="13">The M domain binds the 7SL RNA and the signal sequence of presecretory proteins.</text>
    </comment>
    <comment type="domain">
        <text evidence="13">The NG domain, also named G domain, is a special guanosine triphosphatase (GTPase) domain, which binds GTP and forms a guanosine 5'-triphosphate (GTP)-dependent complex with a homologous NG domain in the SRP receptor subunit srp101. The two NG domains undergo cooperative rearrangements upon their assembly, which culminate in the reciprocal activation of the GTPase activity of one another. SRP receptor compaction upon binding with cargo-loaded SRP and GTPase rearrangement drive SRP-mediated cotranslational protein translocation into the ER.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the GTP-binding SRP family. SRP54 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430297">
        <property type="protein sequence ID" value="CAZ83792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839601.1">
        <property type="nucleotide sequence ID" value="XM_002839555.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGZ9"/>
    <dbReference type="FunCoup" id="D5GGZ9">
        <property type="interactions" value="945"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GGZ9"/>
    <dbReference type="GeneID" id="9185046"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007635001"/>
    <dbReference type="eggNOG" id="KOG0780">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009301_6_1_1"/>
    <dbReference type="InParanoid" id="D5GGZ9"/>
    <dbReference type="OMA" id="GMTGQDA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005786">
        <property type="term" value="C:signal recognition particle, endoplasmic reticulum targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030942">
        <property type="term" value="F:endoplasmic reticulum signal peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006616">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane, translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17875">
        <property type="entry name" value="SRP54_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.30:FF:000003">
        <property type="entry name" value="Signal recognition particle 54 kDa protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000022">
        <property type="entry name" value="Signal recognition particle 54 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.140:FF:000001">
        <property type="entry name" value="Signal recognition particle GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="entry name" value="Signal recognition particle SRP54, nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.30">
        <property type="entry name" value="Signal recognition particle, SRP54 subunit, M-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00306">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036891">
        <property type="entry name" value="Signal_recog_part_SRP54_M_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004125">
        <property type="entry name" value="Signal_recog_particle_SRP54_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022941">
        <property type="entry name" value="SRP54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006325">
        <property type="entry name" value="SRP54_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01425">
        <property type="entry name" value="SRP54_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 54K PROTEIN SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564:SF5">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE SUBUNIT SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02978">
        <property type="entry name" value="SRP_SPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00963">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47446">
        <property type="entry name" value="Signal peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00300">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 13" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="8 13" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="10 13" id="KW-0733">Signal recognition particle</keyword>
    <feature type="domain" description="SRP54-type proteins GTP-binding" evidence="14">
        <location>
            <begin position="269"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU364034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAZ83792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="546" mass="58655" checksum="5ECBF703B18D0A90" modified="2010-06-15" version="1">MVLADLGKRITSAVSDLARSNVVDEKVFDGMLKEICSALIEADVNVKLVSNLRKSIKHTVNFKELNPNVNKKRLIQKAVFDELVKLVDPHSEPFKPKKGRSNVIMFVGLQGSGKTTTCTKLARHYQARGFKACLVCADTFRAGAFDQLKQNATKAKIPYYGSYTETDPVKVSREGVEKFKKERFDIIIVDTSGRHQQEKELFAEMVQIQQAVTPDQTIMVLDASIGQAAEAQSKAFKESANFGAIIITKTDGHAAGGGAISAVAATRTPIMFIGTGEHMLDLERFAPQAFVSKLLGMGDMQGLVEHVQSLKLDQKDTMKHIQEGIFTVRDLRDQLGNIMKMGPLSKMAGMIPGLSQMMGDVGDEEGSMKLKRMIYIMDSMTEKELDSDGKCFSDQPTRMTRIAHGSGTTVREVEDVLTQHRMMAGMAKKMGKNMANMQKQGVGMNGPNKQQQLAAMQRRMQSMGGGLGGPGGGNMAEMMKALGGMPGMGGAGGGGGNPLAGMDMARMMQQFGGMVGGGGGMPDLASMMGSMGGMGGAGRGGRGGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5GH49</accession>
    <name>D5GH49_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00007702001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430297">
        <property type="protein sequence ID" value="CAZ83842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839651.1">
        <property type="nucleotide sequence ID" value="XM_002839605.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GH49"/>
    <dbReference type="STRING" id="656061.D5GH49"/>
    <dbReference type="GeneID" id="9185096"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007702001"/>
    <dbReference type="eggNOG" id="ENOG502SD1I">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096859_1_0_1"/>
    <dbReference type="InParanoid" id="D5GH49"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005786">
        <property type="term" value="C:signal recognition particle, endoplasmic reticulum targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039914">
        <property type="entry name" value="SRP9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039432">
        <property type="entry name" value="SRP9_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12834">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 9 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12834:SF12">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 9 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05486">
        <property type="entry name" value="SRP9-21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SRP9" evidence="2">
        <location>
            <begin position="5"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ83842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="122" mass="13102" checksum="4FE644E5E25F84DB" modified="2010-06-15" version="1">MKLTKNPQEFVDESLLLLNARHSTTIITTSYHAAPTGKGKLTLKTYDPVSGALVKFRTSKIAVVGRLVAGLNRLGRQQAGVSEPTVTETRVAEAPSAPSTYAPIPRQAVTSKSKKKKKKGKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHK6</accession>
    <name>D5GHK6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00007960001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ATG33 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430319">
        <property type="protein sequence ID" value="CAZ83999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839808.1">
        <property type="nucleotide sequence ID" value="XM_002839762.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHK6"/>
    <dbReference type="STRING" id="656061.D5GHK6"/>
    <dbReference type="GeneID" id="9183653"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007960001"/>
    <dbReference type="eggNOG" id="ENOG502S77Q">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065432_0_0_1"/>
    <dbReference type="InParanoid" id="D5GHK6"/>
    <dbReference type="OMA" id="WCLSSPR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051668">
        <property type="entry name" value="ATG33"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37278">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 33-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37278:SF1">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 33-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ83999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="165" mass="17320" checksum="BF732EB5E74BC0CC" modified="2010-06-15" version="1">MPALASLLKFTGVFSLGILSGVHLNFSTATLDAILSLPSASTAQRAFSISLGKQRQVVRPLEIAATASLFTAFLFGGRRGRHGYLIFTGLMPVLGVALEKSRMVGVEVGVVNVQTRKKRGGESVDVNGEVVKGGMEAWRVWGLVRGGLVAFGFAMGVVGIWGDGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>D5GI23</accession>
    <name>D5GI23_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008237001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430322">
        <property type="protein sequence ID" value="CAZ84166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839975.1">
        <property type="nucleotide sequence ID" value="XM_002839929.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GI23"/>
    <dbReference type="STRING" id="656061.D5GI23"/>
    <dbReference type="GeneID" id="9183499"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008237001"/>
    <dbReference type="HOGENOM" id="CLU_306336_0_0_1"/>
    <dbReference type="InParanoid" id="D5GI23"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="454"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="505"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="667"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="856"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="456"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="505"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="675"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="693"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="766"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="805"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="830"/>
            <end position="840"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="967" mass="107364" checksum="EE25B18939FFE766" modified="2010-06-15" version="1">MARTRSISNDRAVGRRARDSMHWNRYSHSPIGSSQRTETEPQTSVRRVKSASPGPALITGRTQRKSNGTTPVLPTPSQRSRVWKNGVVKQRARSRSQRGRSKDGDDVSGPSRDGSMVPAQAHEAQEGDKISRALPDLPEDDVISFASFLDTPRSSIMSRRSGSPPNGPIALQTPGPKKSSTQRELEAMDTELVLESTSELDRNAQRLLDLLILNPPEGDMLVSTHTKDSKVSKRLRVYWLAFGASKIVFGTSHFLDVGTVEMALGGGGYTPVIYRANLAILANFIYTANEEDDTTFQELKSIERYFPTQVGGIIDDQSLQFALDLRTQTVIRGMLSVQEDSLEPNMFLKHFFMERVLASEANDGKPEYKCKPWLGSRDVDEWEGVIIERIRSIRSTFRFEGGQNDGEESHKGRVQVDFEKLTALFPWKGFVGQATEFIKGQLIKLDDSNMGACKEQGSSANKKIVDESQQQVEVSLENRDSFDQDTSQISTEVDKVEYSGLSNFEDQTVDNQNPDSQPEPSSQFVGGGSQLNVRAAKLVKRDLLAREALSKGVRANSIASSIVSNPESIATTVAQVNAIKKRKRKSEIMEITNRQEDGEAQSTTPGYAVSETVVNSLAQMKHLKGAKKLLVQQKQSVPAPLPLALGRQELSGDPDDLLSTNQEGGCEFTQVSEGDRDESDGESSDDPGYRAFSELRREQDKENKKRMTPVPLPSPRRRKRLKRFLDPQEGAMRGSPIDEEGVVILTPPIRSDKSRGNRIRRTLGPGEEEQEDEDGDESEEEQEDEGGDESEEQEDEDEDGSEFVPDPRDVREDNVNRSRRHLPSGFRNGSVSSSDSANSPQSVAVVIDNRLSANPLARRGSSSNVALVPANPSHRSQRNPSEEAEGSQAGPLDRRFDEYSHRSLQQVSRINRLAKINRAAVHTQRRSTTFWTEEGTKALMQAIHEYGPSWAVIRDVSSYPYSPVVEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>D5GI54</accession>
    <name>D5GI54_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">type I protein arginine methyltransferase</fullName>
            <ecNumber evidence="3">2.1.1.319</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">GSTUM_00008281001</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-arginyl-[protein] + 2 S-adenosyl-L-methionine = N(omega),N(omega)-dimethyl-L-arginyl-[protein] + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:48096"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11991"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61897"/>
            <dbReference type="EC" id="2.1.1.319"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:48097"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-arginyl-[protein] + S-adenosyl-L-methionine = N(omega)-methyl-L-arginyl-[protein] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48100"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11990"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65280"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:48101"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.319" evidence="3"/>
    <dbReference type="EMBL" id="FN430323">
        <property type="protein sequence ID" value="CAZ84197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840006.1">
        <property type="nucleotide sequence ID" value="XM_002839960.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GI54"/>
    <dbReference type="STRING" id="656061.D5GI54"/>
    <dbReference type="GeneID" id="9181915"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008281001"/>
    <dbReference type="eggNOG" id="KOG1499">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017375_6_0_1"/>
    <dbReference type="InParanoid" id="D5GI54"/>
    <dbReference type="OMA" id="YSHFAIH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042054">
        <property type="term" value="F:histone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035242">
        <property type="term" value="F:protein-arginine omega-N asymmetric methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.160.11:FF:000016">
        <property type="entry name" value="Protein arginine methyltransferase RmtB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000034">
        <property type="entry name" value="Protein arginine N-methyltransferase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.160.11">
        <property type="entry name" value="Hnrnp arginine n-methyltransferase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049482">
        <property type="entry name" value="ANM3-like_C2H2_Zf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025799">
        <property type="entry name" value="Arg_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055135">
        <property type="entry name" value="PRMT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006:SF116">
        <property type="entry name" value="PROTEIN METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21137">
        <property type="entry name" value="ANM3_C2H2_Zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22528">
        <property type="entry name" value="PRMT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51678">
        <property type="entry name" value="SAM_MT_PRMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 15" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 15" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="7 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="140"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="11"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAZ84197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="527" mass="59839" checksum="8C19007891B6342B" modified="2010-06-15" version="1">MSRSRAPDPPIDTEQDSILNDSDEEGWEDAEADGEEVSIVCLFCTQTFPLSEGVFEHCAAVHGFEYKVVRRELGLDFYGCIKLINYIRSEIAQSRVPDFATTAAFLQDEKYLRPVLEDDALLFGLDGEEGENMKEEDGGLTEDQKRVRELEERLKKLELVHSEYREAVNRNLEMRLEEEDRDVKKGGSEDDSHYFESYSGNDIHEIMLKDSVRTDAYRDFVYGNKHLFKDKVVLDVGCGTGVLSMFCAKVGAKKVIAVDNSAIINKAAANVFENSLDGVITCIRGKIEEVTLPVKQVDVIVSEWMGYVLLYEAMLDSVLFARDKYLAPDGLMVPSECKILIAAMHDSEYMNDSVDFWNHVYGFSMTAMKEKIREDVAITGLNSSSLASEPIAFCHLPLHTIRTSDLTFTKPFQLRIKQNVESLDAFVIYFDTFFATSRNQLVPEDARAESWKHGEGGVAFTTGPFGKLTHWKQGVLLVDRRGGLLKEGEIINGEVTYKKRSGNSREVEVEIEWKTENRKDKQMWYMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIF9</accession>
    <name>D5GIF9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NCT transcriptional regulatory complex subunit B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Negative cofactor 2 B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00008479001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Part of the NCT transcriptional regulatory complex that acts as a key regulator of ergosterol biosynthesis and the azole exporter cdr1B. The NCT complex binds the promoters of genes linked to azole susceptibility, and especially represses the expression of cdr1B transporter.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Forms the NCT transcriptional regulatory complex with nctA and mot1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="CAZ84302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002840065.1"/>
    </dbReference>
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        <property type="interactions" value="826"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GIF9"/>
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    <dbReference type="KEGG" id="tml:GSTUM_00008479001"/>
    <dbReference type="eggNOG" id="KOG0871">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066247_11_3_1"/>
    <dbReference type="InParanoid" id="D5GIF9"/>
    <dbReference type="OMA" id="RDAKFKK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017054">
        <property type="term" value="C:negative cofactor 2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001046">
        <property type="term" value="F:core promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017055">
        <property type="term" value="P:negative regulation of RNA polymerase II transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016480">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd22905">
        <property type="entry name" value="HFD_Dr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000019">
        <property type="entry name" value="Negative cofactor 2 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042225">
        <property type="entry name" value="Ncb2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46138">
        <property type="entry name" value="PROTEIN DR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46138:SF1">
        <property type="entry name" value="PROTEIN DR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00615">
        <property type="entry name" value="CCAATSUBUNTA"/>
    </dbReference>
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        <property type="entry name" value="Histone-fold"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="8">
        <location>
            <begin position="14"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00053814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00065193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00072420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00079659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ84302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="144" mass="16271" checksum="51C55ED4AA2CA2DB" modified="2010-06-15" version="1">MSDREFGGGADDLSLPKATVQKIISEILPNDLAFAKETRDLLIDCCVEFITLVSSEANDIAEREAKKTIAAEHVVKALKDLGFEEYIEQIQEVAQEHKEHQRSREKKQTKLEQSGLSQEELLRQQEELFGNARAKFNQQGSVPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIR3</accession>
    <name>D5GIR3_TUBMM</name>
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        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">GSTUM_00008621001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
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        <lineage>
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            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
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            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430328">
        <property type="protein sequence ID" value="CAZ84406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840215.1">
        <property type="nucleotide sequence ID" value="XM_002840169.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIR3"/>
    <dbReference type="GeneID" id="9185624"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008621001"/>
    <dbReference type="HOGENOM" id="CLU_2905804_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIR3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072816" evidence="1">
        <location>
            <begin position="22"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="62" mass="6576" checksum="23F2258A6300AC29" modified="2010-06-15" version="1">MPMIELLVLVQLLIACGVGRGEGVGSSDDGFPTASWGAGDAYWNPDTTHDAVLEQVTKEHYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIS5</accession>
    <name>D5GIS5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008646001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430328">
        <property type="protein sequence ID" value="CAZ84418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840227.1">
        <property type="nucleotide sequence ID" value="XM_002840181.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIS5"/>
    <dbReference type="GeneID" id="9185636"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008646001"/>
    <dbReference type="HOGENOM" id="CLU_2265678_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIS5"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="103" mass="12091" checksum="AAC344AA553D5953" modified="2010-06-15" version="1">MTFLHLYFSLSSCLLKKHPWASLFLLLLNFYFLLLVSLHIFFLSAQGCSLLHVIFFLLPSSLFPPSLNIFIRIFCMHRDVPFIHLSSHQNVINFSDECREKTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIZ3</accession>
    <name>D5GIZ3_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">pyridoxal kinase</fullName>
            <ecNumber evidence="2">2.7.1.35</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00008749001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the pyridoxine kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.35" evidence="2"/>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840295.1">
        <property type="nucleotide sequence ID" value="XM_002840249.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIZ3"/>
    <dbReference type="FunCoup" id="D5GIZ3">
        <property type="interactions" value="361"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GIZ3"/>
    <dbReference type="GeneID" id="9181827"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008749001"/>
    <dbReference type="eggNOG" id="KOG2599">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046496_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIZ3"/>
    <dbReference type="OMA" id="WEGLKQS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008478">
        <property type="term" value="F:pyridoxal kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009443">
        <property type="term" value="P:pyridoxal 5'-phosphate salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01173">
        <property type="entry name" value="pyridoxal_pyridoxamine_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013749">
        <property type="entry name" value="PM/HMP-P_kinase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004625">
        <property type="entry name" value="PyrdxlKinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00687">
        <property type="entry name" value="pyridox_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10534">
        <property type="entry name" value="PYRIDOXAL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10534:SF2">
        <property type="entry name" value="PYRIDOXAL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08543">
        <property type="entry name" value="Phos_pyr_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyridoxamine kinase/Phosphomethylpyrimidine kinase" evidence="7">
        <location>
            <begin position="82"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ84486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="335" mass="36615" checksum="49A3416031616533" modified="2010-06-15" version="1">MMEDRKVLTIASHVCVGYVGNSVTTFVLQLMGLDVASLNTVQFSNHAGYRRLKGFRTTAGQISDLYDGLKGCGLDDFGMLLTGYIPSEECVEVVGRIAGDMKGREGGCFWLLDPVMGDQDRLYVSEGVLPVYKSLVRVADLIVPNQFEAELLSGVKVDSLTSLSRAISELHRIYNVPHVIITSVTFTNGDKKMLCAGSSATSSGVPRKFMFNVEIIDGFFSGTGDMFAALTLARFREEAEKGGLEAAQSWRCDDAVGPLELPLCKAIGRVLGSMHLVLVQTQLARDRVLGKKVKEEVEVGSEEYVRLTKASELRLVQCQSELKDPQIGYEAVVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJ02</accession>
    <name>D5GJ02_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">GSTUM_00008761001</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840304.1">
        <property type="nucleotide sequence ID" value="XM_002840258.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJ02"/>
    <dbReference type="STRING" id="656061.D5GJ02"/>
    <dbReference type="GeneID" id="9181836"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008761001"/>
    <dbReference type="eggNOG" id="KOG0800">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_426539_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJ02"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45977:SF4">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45977">
        <property type="entry name" value="TARGET OF ERK KINASE MPK-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="14">
        <location>
            <begin position="167"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="116"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="235"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="517"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="254"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="264"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="517"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAZ84495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="642" mass="72140" checksum="B1D23468E28A0148" modified="2010-06-15" version="1">MSSHEFHSGHHNPEDGAAAGLLNDISSYTFQTLLHGPSSYRTSSTSSHVFYNAPARPYAPLPTHPHAPLSTTYGTPIRSQVMRQSFVAYDDVSFQDYEIPPTRPFVRGRNFYASPGSWQSEEPPVNPRASSPPRMVLPKRKRHASEKALAELKPLALRELKEDETACIICMEDYVDVPDSTDELKTEHALQMGCGHHFGSYCLKIWLKGHSTCPTCRREVDFVEEEEGRSMAMSRRWQSHQEMRRQGAHAAAAARERNGPDSTRHHHAMGHRRRISTQSVDITARRVSPQSPSWLERDQSNHPGIRSDANPPEDSRVSQAPRLQPVDQQGNGNGSGQVPYREAPVPHIPTGISPIRFGDTPLFTANMDMALCMASPFLRHAPIINALPPRHPVHTPMTQRPQSMHPQAHQSLYSNLDMGINRAPAPQPERLPPGIPVTRGGPHHQPYRHIPASPPPTPMAPTHQAPPNYSIQPCVQHNDYSNNEPQQMQYPEYRDQHWQAHQHQMSTMLPPPNTTLNSNANINSINNSNTNSTTAPTTTTTSEDDLQQHEQHQHQQTQCNLTEIGFCIEPFYTGRTQGNLPAILGLDCGHSYHVICLVSITKSRPQQPVNRRLAWCQRCNRFEMLVGGELPDFTWEEVESFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJI8</accession>
    <name>D5GJI8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Calmodulin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00009035001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430332">
        <property type="protein sequence ID" value="CAZ84681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840490.1">
        <property type="nucleotide sequence ID" value="XM_002840444.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJI8"/>
    <dbReference type="FunCoup" id="D5GJI8">
        <property type="interactions" value="188"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJI8"/>
    <dbReference type="GeneID" id="9182682"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009035001"/>
    <dbReference type="eggNOG" id="KOG0027">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061288_13_1_1"/>
    <dbReference type="InParanoid" id="D5GJI8"/>
    <dbReference type="OMA" id="HDQASTN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071341">
        <property type="term" value="C:medial cortical node"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110085">
        <property type="term" value="C:mitotic actomyosin contractile ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903475">
        <property type="term" value="P:mitotic actomyosin contractile ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000003">
        <property type="entry name" value="Calmodulin A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF0">
        <property type="entry name" value="CALMODULIN LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="50"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="123"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00020786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ84681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="190" mass="21050" checksum="DD25959ACFF77434" modified="2010-06-15" version="1">MSKKVLENNLLTSRSSTSPTSAFHPVPSSPPSHQLAPPSFFFLLEYYTLPATTNYKEAFALFDKRGNGRIPLSSLGDLLRACGQNPTNAEVDDLIHGSAPKGDLDFESFLKILNRPNGFRDPGEPDEFVRGFQVFDKDCTGYIGVGELRYVLTSLGEKLTNEEVDELLKGMNVHGDQVNYTDFVKMILAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJM1</accession>
    <name>D5GJM1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Replication factor C subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00009072001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the activator 1 small subunits family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430332">
        <property type="protein sequence ID" value="CAZ84714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840523.1">
        <property type="nucleotide sequence ID" value="XM_002840477.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJM1"/>
    <dbReference type="FunCoup" id="D5GJM1">
        <property type="interactions" value="753"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJM1"/>
    <dbReference type="GeneID" id="9186748"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009072001"/>
    <dbReference type="eggNOG" id="KOG0990">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042324_2_0_1"/>
    <dbReference type="InParanoid" id="D5GJM1"/>
    <dbReference type="OMA" id="AEDNLPW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031390">
        <property type="term" value="C:Ctf18 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005663">
        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031391">
        <property type="term" value="C:Elg1 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031389">
        <property type="term" value="C:Rad17 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18140">
        <property type="entry name" value="HLD_clamp_RFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000028">
        <property type="entry name" value="Replication factor C subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.272.10:FF:000004">
        <property type="entry name" value="Replication factor C subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000129">
        <property type="entry name" value="Replication factor C subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050238">
        <property type="entry name" value="DNA_Rep/Repair_Clamp_Loader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013748">
        <property type="entry name" value="Rep_factorC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047854">
        <property type="entry name" value="RFC_lid"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001679">
        <property type="entry name" value="PRK00440.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669">
        <property type="entry name" value="REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669:SF9">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08542">
        <property type="entry name" value="Rep_fac_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="8">
        <location>
            <begin position="75"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00070184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ84714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="387" mass="42637" checksum="4EF2D804BA8949A6" modified="2010-06-15" version="1">MSDIDEMELDEEHAATNDVVFSSGQKGKKLSKANLPVSAGDTLPWVEKYRPDTLNDVSGHEDILTTINKFVQTNRLPHLLFYGPPGTGKTSTILALARHIYGAHNIRQQVLELNASDDRGIEVVREQIKTFASTKQIFGASTKTDGELGSFKLIILDEADAMTSTAQNALRRIMEKYTANTRFCIIANYTHKLNAALLSRCTRFRFSPLPIDALRRRVDHVIDAEKVKITPSAVDALLQLSRGDMRRSLNVLQACHASSTPLDDKGRPDPTAEREDITETHIYDCIAAPHPEDVQIILKTLLSSDITTSLRTIIDIKTKKGLALADLLSSLSDELERLEVPKQTRILWMEGLAEVEWRVGSGGSEGIQTGALAGAVRKGVAVMKNGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJZ7</accession>
    <name>D5GJZ7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00009287001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430335">
        <property type="protein sequence ID" value="CAZ84840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840649.1">
        <property type="nucleotide sequence ID" value="XM_002840603.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJZ7"/>
    <dbReference type="GeneID" id="9186876"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009287001"/>
    <dbReference type="HOGENOM" id="CLU_1050500_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJZ7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="265" mass="29350" checksum="C3C152981244FE59" modified="2010-06-15" version="1">MSRIKRNIAPSRRKQRSQRKQVDSTDRKDEEIYECIVVRFPGEIGARGEAREGVSTPKMTDGVAQAMDSAEHVTCPNDTLPVRASGARNTRSRAQTRVSAQESEKSAFTPVRGTRARRSACGNRIPKAPYEETNGVFTPPQPAATRKGKERVADTPETNPPARRGDRDTSNHDSAPTAETPKPALRRSTRKIPVSYYPPAETKRSLARPAKRPRAQVSRDSGAIMFRSEIDYDIPTEFEESNVRADLTDRPAVPVGRGWGLSRGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5GK30</accession>
    <name>D5GK30_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00009337001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430335">
        <property type="protein sequence ID" value="CAZ84873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840682.1">
        <property type="nucleotide sequence ID" value="XM_002840636.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GK30"/>
    <dbReference type="STRING" id="656061.D5GK30"/>
    <dbReference type="GeneID" id="9186910"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009337001"/>
    <dbReference type="eggNOG" id="KOG1178">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002220_2_1_1"/>
    <dbReference type="InParanoid" id="D5GK30"/>
    <dbReference type="OMA" id="WDWLAHI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031177">
        <property type="term" value="F:phosphopantetheine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051414">
        <property type="entry name" value="Adenylate-forming_Reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013120">
        <property type="entry name" value="FAR_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020806">
        <property type="entry name" value="PKS_PP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43439:SF2">
        <property type="entry name" value="ENZYME, PUTATIVE (JCVI)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43439">
        <property type="entry name" value="PHENYLACETATE-COENZYME A LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23562">
        <property type="entry name" value="AMP-binding_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07993">
        <property type="entry name" value="NAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00823">
        <property type="entry name" value="PKS_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carrier" evidence="3">
        <location>
            <begin position="554"/>
            <end position="631"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ84873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1041" mass="114696" checksum="72F41912E162B6CA" modified="2010-06-15" version="1">MPPKIENTPAPVDETPLTIDHLLRLRASQIPDTPILAYSHNKKEYTQYTASQLNALAYRVAKHYKKLITQCASSEAPERVVALLGISDFDYVISLLALAKLGMAVLLLSTRISDEAYRHLFDRTGCSDVIVQPTFEKTMGRVRSGHGSALNVVCMASRGLYEPEVAPEEEKGLTLEDMRFDTQLDMGVESSKNAFIIHSSGSTGLPKPVGQTHRTVIGNASAISMNPTSSFSTLPLFHTFGLTAMFGAIYYGKVTSIPNADLPITGKNMIAGLKATRPETFYLVPYTLKLMAETDEGMEAMKICKSVCSGGSACPEELGDKLAAAGITLVSYYGSTEGGALLNSYRPESDKDWNYLKAIPPALPHIRWEKYSESVYELVLLAGWHAKAFTNNPDGSYSTRDLWQQHPTDPLKWKYVGRLDDTIVLSNGEKASPLALEGSVRDSPYVNEVVCVGVQQPTLGLLVIPTERTIGMSRAELIQKMRPSIEAGNVRMPAYAQISAEMIGFLPIGTPYPATDKGTVIRPAFNRTFRVQIESMYSRYEENNASEGLSLSEEELRAYVRNTIIKTLKLEDTTALPDDTDFFSLGLDSLGSMQVQGSIRRELNTGKQIGQNVVFEKPTVRKLAGHLYRLRTGEVEKEEDEQSQIEIMKGLVEKYSHFEQHVPGNSDKQGDYIIVTGATGSLGAHLVSQLATKPTTQKVYCLVRAKGQPNAHERTIQSLKLRRVYEDLSATSLSKIIALPSDFSDPRLGLSEDVYAELLDSVNIVIHCAWQVNFNMDVSSFENAHIKGSHNLMTLCLKSHRSSPASFNFCSSISSVANNSTAETIPETQPKYENAMPMGYARSKLVTEKLCDVAARRTTLSARVLRIGQVSGDTRFGIWNATEAIPLTVRSAKTLGALPAPVNDEVVSWVPVDVAAGTIIDLSCLPQPAQNEASVFHVSHPRLLSWNNDFLPALRVAGLEFESVKPTEWIRRLKAVAQDADVNPEVKLLGFFEGKYTSDEVRSAPYFETVKACAASRALSGVEDIKDDIVGRYLEFWREGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>D5GK57</accession>
    <name>D5GK57_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00009374001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430336">
        <property type="protein sequence ID" value="CAZ84900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840709.1">
        <property type="nucleotide sequence ID" value="XM_002840663.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GK57"/>
    <dbReference type="STRING" id="656061.D5GK57"/>
    <dbReference type="GeneID" id="9186944"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009374001"/>
    <dbReference type="eggNOG" id="KOG1414">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_687335_0_0_1"/>
    <dbReference type="InParanoid" id="D5GK57"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14687">
        <property type="entry name" value="bZIP_ATF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000053">
        <property type="entry name" value="BZIP transcription factor AtfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051027">
        <property type="entry name" value="bZIP_transcription_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002112">
        <property type="entry name" value="Leuzip_Jun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19304">
        <property type="entry name" value="CYCLIC-AMP RESPONSE ELEMENT BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00043">
        <property type="entry name" value="LEUZIPPRJUN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="8">
        <location>
            <begin position="283"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="201"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="301"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="201"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ84900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="401" mass="43740" checksum="EE21CFF347E5BF72" modified="2010-06-15" version="1">MQVGLTPKQSGIMSVLTSQSSAIVFGSPSDTRAPVQHFRGGENTRGTAEQDAIILANRKSSHNRQSFEPRNNLDGWPSDGFLGLHPPEQEQLDSYDGVDTADNTANYLFLLANAQRQPQDTIGAFHSDLNAPAALRYPRGPACSSGAMVDTSCPVPSQGLLINSSSDAAMGANRNRNISGHSIPTSIVGINDNSNVIIESSHHERQDTGSRWDRETKNSQEMISGPTSVRRKAENGIQGTVRSRVKKAKGRDGQAISLSGGICDEQDGRDDENGKGTAKMTDEEKRKSFWERNRLAALKCRERKKKWIANLEEKVERFSRENAKLSAQVISFREEIVSIRTLLLAYSDCPVARANTGSMSTPNRNSGTYNEYGHMTTGYYDITTCMGGGNIPHLPMGGGYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5GK63</accession>
    <name>D5GK63_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00009389001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the SPT3 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430336">
        <property type="protein sequence ID" value="CAZ84906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840715.1">
        <property type="nucleotide sequence ID" value="XM_002840669.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GK63"/>
    <dbReference type="FunCoup" id="D5GK63">
        <property type="interactions" value="892"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GK63"/>
    <dbReference type="GeneID" id="9186939"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009389001"/>
    <dbReference type="eggNOG" id="KOG3902">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038706_1_1_1"/>
    <dbReference type="InParanoid" id="D5GK63"/>
    <dbReference type="OMA" id="QFQIDAP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22926">
        <property type="entry name" value="HFD_SPT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000023">
        <property type="entry name" value="transcription initiation protein SPT3 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003195">
        <property type="entry name" value="TFIID_TAF13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11380">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID/SUPT3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11380:SF16">
        <property type="entry name" value="TRANSCRIPTION INITIATION PROTEIN SPT3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02269">
        <property type="entry name" value="TFIID-18kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="82"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="82"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ84906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="293" mass="33499" checksum="BC59DD5A1617FE39" modified="2010-06-15" version="1">MMFVSGETCEPSAETAGMVEEIVRGQIIEMLQKCSSIASRRGSRSLSTQDLIFLMSHDIGKASRLRTYLSWKYVRKSARDNDPSLGAEVHDSGDHPDDPVGPTTMGQKRVRTALPWGIASSFSEQFDETEEGEDEDAVEENAATLQRLRNADERTRGMTRDQYVHWSRCRQASFTYRKGKRFRDWVGIRTITDATLDDDIIDILGFLAFEIVQTLTEEALEVKRLEHKVSLGAREESKKRKRGNLWFSAEEQRTPIEQRHIVEAFRRLQSPKPKWRAMKNLSAGVFKSKLTLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKC5</accession>
    <name>D5GKC5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00009474001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430337">
        <property type="protein sequence ID" value="CAZ84968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840777.1">
        <property type="nucleotide sequence ID" value="XM_002840731.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKC5"/>
    <dbReference type="STRING" id="656061.D5GKC5"/>
    <dbReference type="GeneID" id="9181988"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009474001"/>
    <dbReference type="eggNOG" id="KOG2078">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_364548_0_0_1"/>
    <dbReference type="InParanoid" id="D5GKC5"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002939">
        <property type="term" value="P:tRNA N1-guanine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.110">
        <property type="entry name" value="Met-10+ protein-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030382">
        <property type="entry name" value="MeTrfase_TRM5/TYW2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056743">
        <property type="entry name" value="TRM5-TYW2-like_MTfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245:SF36">
        <property type="entry name" value="TRNA (GUANINE(37)-N1)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245">
        <property type="entry name" value="TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02475">
        <property type="entry name" value="TRM5-TYW2_MTfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51684">
        <property type="entry name" value="SAM_MT_TRM5_TYW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="SAM-dependent methyltransferase TRM5/TYW2-type" evidence="7">
        <location>
            <begin position="136"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="475"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="656"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="714"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="719"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="756"/>
            <end position="766"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ84968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="766" mass="86185" checksum="1A864C223A6FFA3F" modified="2010-06-15" version="1">MFMSRFLPPVDRAMRTLDRAFFRKVVPVTAARVLDSALVHTIKEKILKDLIPFERFNQRYDFNSKLNVETPLLILQPQVKYGDLSTASGPTQEYVKSKILELVPYDLKLEYGDWGYEEIMKAVLPEDVNPPSIDDFPRIGPLLHLDVPEPLREYRNLIAEVICDKEREVQTVFGEPEKVGGALELGLANEVLAGPENYRVELSHQGSSFTFHLDQAGFRDVYFDTKLATERQRLVDTFGLGECVADVYSGVGALSVAAAKRGAIVFANERRDKVYQLLVENRRNNKVKKNLYLKSQAPARFVQQLVRKLFEKTVDYKPQKISKLGKLIKNPLKKGDKNLPACVSHIVFSDPDQSLTHLECLRGIYTKRERLFANRGGSFWKLDFPFVHVYAYHKAPAEKKADAVNDLLKKIGKRIGHDLRRGCVQQAHFVKKGSEDNLYCITFKLPPPVAFDIREEELHQKQPEMVDLAASKEAAISKSDDRQPGRISVHRTYLPSRDPDINRFGPSGRPPSQVEGREEKGLEPTPFPGAPTQIAPSATPPTGLPAHSPASITTPPRHLVYTPLASFQPRDAGSVATKQSQGAPTDRPPMGGGPSMTSNLGLIKTESELAKEFAKTYKVPRGLPPWKQKVHRKEKKAAWMARQTRKQQEQAYLTQGSFNNPHRNDNGQRGPEYWASSRGERRFHSDNSPTQPGYGPYGYTECVAESVDRHSVSQLAAGSKDDGHANREETGPSRPLIRFHIAANKTPKGGPMIRWHQVDTHPKMFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKR9</accession>
    <name>D5GKR9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00009735001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430340">
        <property type="protein sequence ID" value="CAZ85112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840921.1">
        <property type="nucleotide sequence ID" value="XM_002840875.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKR9"/>
    <dbReference type="STRING" id="656061.D5GKR9"/>
    <dbReference type="GeneID" id="9183348"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009735001"/>
    <dbReference type="HOGENOM" id="CLU_1972079_0_0_1"/>
    <dbReference type="InParanoid" id="D5GKR9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042602">
        <property type="term" value="F:riboflavin reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002563">
        <property type="entry name" value="Flavin_Rdtase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050268">
        <property type="entry name" value="NADH-dep_flavin_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30466">
        <property type="entry name" value="FLAVIN REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30466:SF1">
        <property type="entry name" value="FMN REDUCTASE (NADH) RUTF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01613">
        <property type="entry name" value="Flavin_Reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavin reductase like" evidence="2">
        <location>
            <begin position="4"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ85112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="127" mass="13959" checksum="A6D55F750741CA7E" modified="2010-06-15" version="1">MAASAMSISGVFTVHVLRSTTRAAHIASLFSKAKLPTSPWESVRDSQLREQGLLGKDEDVLYRLRCETYRSVQIEDHRFWVGRVVGVDFLVGEKEAGFGLMYAEGMFRDVGREVGSDEGGDVDDGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKW2</accession>
    <name>D5GKW2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Replication factor C subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00009792001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the activator 1 large subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430341">
        <property type="protein sequence ID" value="CAZ85155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840964.1">
        <property type="nucleotide sequence ID" value="XM_002840918.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKW2"/>
    <dbReference type="FunCoup" id="D5GKW2">
        <property type="interactions" value="989"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GKW2"/>
    <dbReference type="GeneID" id="9183391"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009792001"/>
    <dbReference type="eggNOG" id="KOG1968">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003574_1_1_1"/>
    <dbReference type="InParanoid" id="D5GKW2"/>
    <dbReference type="OMA" id="LICNERN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005663">
        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902983">
        <property type="term" value="P:DNA strand elongation involved in mitotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070914">
        <property type="term" value="P:UV-damage excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17752">
        <property type="entry name" value="BRCT_RFC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18140">
        <property type="entry name" value="HLD_clamp_RFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000021">
        <property type="entry name" value="Replication factor C subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.272.10:FF:000005">
        <property type="entry name" value="Replication factor C subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000001">
        <property type="entry name" value="Replication factor C subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000395">
        <property type="entry name" value="Replication factor C subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013725">
        <property type="entry name" value="DNA_replication_fac_RFC1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012178">
        <property type="entry name" value="RFC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047854">
        <property type="entry name" value="RFC_lid"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23389">
        <property type="entry name" value="CHROMOSOME TRANSMISSION FIDELITY FACTOR 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23389:SF6">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25361">
        <property type="entry name" value="AAA_lid_RFC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08519">
        <property type="entry name" value="RFC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036578">
        <property type="entry name" value="RFC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 10" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRCT" evidence="13">
        <location>
            <begin position="311"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="946"/>
            <end position="1025"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="400"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="53"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="71"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="101"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="178"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="191"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="235"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="250"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="271"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="954"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="973"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="1009"/>
            <end position="1025"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR036578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ85155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1025" mass="113614" checksum="131932E4EC803019" modified="2010-06-15" version="1">MSADIRNFFTPKGGQPVAGKSKPAPTPTPASRRASARKRRVVEDSDDEEKPAKKITKKPTPRKNTKENRAEPTTTSTYFASTGKNKILRTSPLKPKKPAPEEEYFPNDDEFDEGIYAEEFKKLEDDYKEDEGEDTKASFAVVVEKNGPAKSKANTSPPAKKQKFSLPKGKGVDVDSFVVDDEEEEDEYVKPKRRSAATSATRKRRAAQSHYEHGDDDDYDEIERGEPPAKASKPAAKKPAAKKAKAVPRKSKEAPQESDSVRKILDSVPIFRPPTPPPPTDGEPKKFNYRDFKQRQGAVPIAPGSKEIPVGQENCLTGLTFVFTGILESLAREDGQQLVKKYGGKITSAPSTKTSYVVLGSDAGPKKLATIHKLKIKTINEDGLFKLISSLPANGGDGLAARANEAKKAAEEQKVRELAKEMDDETKSRGKEAIEKEATELWTVKYAPSQLTQICGNKGQVEKLGRWLRNWPKNLKTGFRMRGADGMGGARAVMIHGPPGIGKTTAAHLVAKLEGYDIVESNASDTRSKKLMEEKLRGVLDNRSLLGYFAGDKEKVKHSKQKMVLIMDEVDGMSAGDRGGVGQLAAVCKKSNIPIICICNERKLPKMRPFDMVTYDLPFRRPDAAAVRSRIMSIAYREGIKLPPNVIDQLVEGTHADIRQIINMLSTYSTTQSSMTFDQGKDLAKAWEKHVVLKPWDIAQKLLGNEMFAPTSKKTLNDKIELYFNDHEFSYMMVQENYLKTNPARANNYHGKERNLKMLELVENASESISDGDLVDALIHGPQQQWSLMPAHGMFSTVKPASYMYGGFGGQHFCFTSWLGNNSKQGKLSRFVKEIQSHIRLRASASPRTNLSMLAKRLEREGKDAIPEVIDTMDDYFLTKDDWDAIVELIVGPASALDIPTLTKSSFTRQYNSMSHRMPFMKSSSVAAPKAGKQDAPDLEDVIVESEDEGGDEDIIKKQVEEEQDLSKDKYVKAKKPKATPKGKGKGKAASKKSQNEGEDEDEDDEPKRKPKSASKPRAKAKGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>D5GL04</accession>
    <name>D5GL04_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00009860001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430342">
        <property type="protein sequence ID" value="CAZ85197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841006.1">
        <property type="nucleotide sequence ID" value="XM_002840960.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GL04"/>
    <dbReference type="FunCoup" id="D5GL04">
        <property type="interactions" value="699"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GL04"/>
    <dbReference type="GeneID" id="9185791"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009860001"/>
    <dbReference type="eggNOG" id="KOG0732">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000536_6_1_1"/>
    <dbReference type="InParanoid" id="D5GL04"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006337">
        <property type="term" value="P:nucleosome disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045815">
        <property type="term" value="P:transcription initiation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001218">
        <property type="entry name" value="AAA family ATPase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000061">
        <property type="entry name" value="ATPase family, AAA domain-containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045199">
        <property type="entry name" value="ATAD2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23069">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23069:SF0">
        <property type="entry name" value="TAT-BINDING HOMOLOG 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="9">
        <location>
            <begin position="19"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="9">
        <location>
            <begin position="305"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="460"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="666"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="755"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="849"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="487"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="666"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="769"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="787"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="858"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="901"/>
            <end position="940"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ85197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1036" mass="114383" checksum="98ADEE4E3AE9DA2A" modified="2010-06-15" version="1">MVQLPLTYPELFLAKKITPPRGVLFHGPPGTGKTLMARALAASCSTEARKVTFYMRKGADCLSKWVGEAERQLRLLFEEAKNNQPSIIFFDEIDGLAPVRSSKQDQIHASIVSTMLALMDGMDGRGQVVVIGATNRPDAVDPALRRPGRFDREFYFPLPSVDARKSIIDIHTKGWSPALPEALKEGLALQTKGYGGADLRAITVDKLALDPTSIHVTARDFMLAMKKVVPSSQRSSSSGTDPLPSFIEPLLIGPLQQLKKCMDGILPERKRLTVLEEAMYEDDQDSDGGFSRENMMQDFDQARVFRPRMLIHGAPGMGQQYLAAALLNYFEKLHVQAFDLATLVGDSARSVETAIVQLFVEVKRHKPSVVFIPDVDTWYSSIGQQGLITFKGLLRSIPANDPVLLLGVTNSELDDLDPTLLHDLFGFSKRDRFRIPMPNEEARRKYFDRLISYIQKAPNEFPPDPSTRRKRELPVLPVVPPPPPRAMTKEERKAQETKDKQIKNWLKMRLFYAIDTVRSKLRRFKKPILDYEAIKHLFEEPEVTSDLAIQGTASNYQLLLPKNIHSPPKVIDTTTGKTFYNMDMDVIEERVSNGYYCSAKQFLRDVEYIRDDWMELGHKEHRMKVNELLTHLEVIATDIEVMDQAMAAQCAELWARERKKIEERAEKAERQKRAEGEARLEAEKTRASLDRQSPPAERQIQGGSDKSQKRQATPIHAVGNGEDVSVSASATAVISTTYPPSNGVGVYSHMSSSVEVQTSQVSKNDDLTNTHTRTSSRQGSPAALHSPPSQHTQSSEHASAFQTAISSIPANFSQSVAADGIPQMTSQPGAALGVTPVTGCSVIFNYASTTDSGKRTSDGTAGLNSQPFSNPYSNSNGTPRPMNPPLFHNFGGISTGDSQLPDTQVVPSSSSDSAPSQTSTATPAPHSQPSSQPHLHNSSHPAPPLFPLLPIQTTITCDRTLLANLHDRLVAETGGYTVEQLEQVSSACMSKVWEMRACWDRDEIIRRVGEVFEELDGDIREGGGIGDGSFGSAQLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLB4</accession>
    <name>D5GLB4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00010119001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430348">
        <property type="protein sequence ID" value="CAZ85307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841116.1">
        <property type="nucleotide sequence ID" value="XM_002841070.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLB4"/>
    <dbReference type="GeneID" id="9182101"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010119001"/>
    <dbReference type="eggNOG" id="KOG2533">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_0_1_1"/>
    <dbReference type="InParanoid" id="D5GLB4"/>
    <dbReference type="OMA" id="FKHPTTY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000013">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000188">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF50">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G00840)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="93"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="152"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="182"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="204"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="274"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="312"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="342"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="368"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="401"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="434"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="56"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ85307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="493" mass="54770" checksum="AE46E7DFD75B9656" modified="2010-06-15" version="1">MVERNNKPPELDDRSTSVSEEGDCGVRVGEDVEALTARVIDSISERALCRRFDLRLLPVLAVMYLFNALDKGNLGNAKTNGFEKDLGFTGNQYNILLSVFYVPYVLCAPLVGMLGKMYGPSRVLPISMLTFGSMTLLGAACKTFGGMMTVRVILGVAESAFFPLVIYYLTTFYRRGELARRLAIFYAARLLAFGVFHIKSHLPAWRYLFIIEGTCTVLFSFFAYWYLPMSAAKAHFLSPEEKELAFYRIQVDSSSTVDEEFSLRESIKVFKMPVAWAWLAIEICLGVPLQSVSLFLPQIVERLGYSVVKTNLYTVAPNITGAVMLLVLAFTSDFTRLRSPFITLAFSFPLVGFIIYASIDVLHDEQTAYFATFMMCWGTSAPSVLLSTWYNNNIAHEGKRVVLTSVGVPVANMMGVVSSNIFRPQDAPDYIPALITTACFGAVGTLLAGSLGTYMAFDNSRRNRVQGMDIDARDVATKALKDGPASPNFRWFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLB8</accession>
    <name>D5GLB8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Carboxypeptidase</fullName>
            <ecNumber evidence="6">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00010123001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the peptidase S10 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="6"/>
    <dbReference type="EMBL" id="FN430348">
        <property type="protein sequence ID" value="CAZ85311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841120.1">
        <property type="nucleotide sequence ID" value="XM_002841074.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLB8"/>
    <dbReference type="STRING" id="656061.D5GLB8"/>
    <dbReference type="MEROPS" id="S10.014"/>
    <dbReference type="GeneID" id="9182105"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010123001"/>
    <dbReference type="eggNOG" id="KOG1282">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008523_12_3_1"/>
    <dbReference type="InParanoid" id="D5GLB8"/>
    <dbReference type="OMA" id="LMSTCDF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF479">
        <property type="entry name" value="CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 6" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ85311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="405" mass="46095" checksum="9339E5AB4C83ECF3" modified="2010-06-15" version="1">MPISKDKNETRELYFWFFPTTGTVDDELVIWLNGGPGCSNLEGFLQEHGPFSWRYGTYKPARNRYSWNNLTNMIWVEQPVGTGFSQGVPDATSEEDAAEKFLGFLENFIKTFGFEDKKIYVTGESYAGYYVPYIVDAMYSRNNKTLFDPRGLIIYSPTLSEWVIHEQIPAVQFVDYWAHLLALNKTTMDHLHATADKCGYTSYLKENLLNTSTECDVWSAIFDAALLVNPCFNIFHITATCPILWDVLGYPGSFVYLPEGAEIYFNRTDVQNAINAPHVKWQECSEGVFPNGDPSDPPNFSVLPRIIDKNERTIIAHGLLDFRFTVNGSLLAIQNMTWGGKRGFQTKPVEPFIVPYDSQGTMGVQHTERKLTWVEIESCGHMAPQFQPGAAYRQLEYLLGRIDKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLE5</accession>
    <name>D5GLE5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00010158001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430348">
        <property type="protein sequence ID" value="CAZ85338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841147.1">
        <property type="nucleotide sequence ID" value="XM_002841101.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLE5"/>
    <dbReference type="FunCoup" id="D5GLE5">
        <property type="interactions" value="1235"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GLE5"/>
    <dbReference type="GeneID" id="9186082"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010158001"/>
    <dbReference type="eggNOG" id="KOG0730">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_12_3_1"/>
    <dbReference type="InParanoid" id="D5GLE5"/>
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    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="C:nucleus"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0034098">
        <property type="term" value="C:VCP-NPL4-UFD1 AAA ATPase complex"/>
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    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:autophagosome maturation"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0051228">
        <property type="term" value="P:mitotic spindle disassembly"/>
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    <dbReference type="GO" id="GO:0071630">
        <property type="term" value="P:nuclear protein quality control by the ubiquitin-proteasome system"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:retrograde protein transport, ER to cytosol"/>
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    <dbReference type="CDD" id="cd19519">
        <property type="entry name" value="RecA-like_CDC48_r1-like"/>
        <property type="match status" value="1"/>
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    <dbReference type="CDD" id="cd19528">
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        <property type="entry name" value="Cell division control 48"/>
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    </dbReference>
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        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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    </dbReference>
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        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005938">
        <property type="entry name" value="AAA_ATPase_CDC48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
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        <property type="entry name" value="AAA_lid_3"/>
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    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
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        <property type="entry name" value="ATPase_AAA_core"/>
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        <property type="entry name" value="ATPase_AAA_CS"/>
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    <dbReference type="InterPro" id="IPR004201">
        <property type="entry name" value="Cdc48_dom2"/>
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    <dbReference type="InterPro" id="IPR029067">
        <property type="entry name" value="CDC48_domain_2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003338">
        <property type="entry name" value="CDC4_N-term_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01243">
        <property type="entry name" value="CDC48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF171">
        <property type="entry name" value="NUCLEAR VALOSIN-CONTAINING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02933">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02359">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01072">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01073">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54585">
        <property type="entry name" value="Cdc48 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CDC48 N-terminal subdomain" evidence="7">
        <location>
            <begin position="39"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="CDC48" evidence="6">
        <location>
            <begin position="139"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="251"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="525"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="783"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="799"/>
            <end position="809"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ85338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="818" mass="89625" checksum="DEE90AF564E4480D" modified="2010-06-15" version="1">MADHEEKKKVNLADASGATHKEADETATAILKKKKKPNSLIVTDAVNDDNSIIALSNNTMETLQLFRGDTVLVKGKKRKDTVLIVLADDDLDDGSVRINRVVRNNLRIRLGDVITVHPCPDIKYAKRIAVLPIADTVEGLTGSLFDVYLKPYFLEAYRPVRMGDLFTVRGGMRQVEFKVVELDPPDYGIVAQDTVIHCEGEPLNREDEEGNLNEVGYDDIGGCRKQMAQIRELVELPLRHPQLFKSIGIKPPRGILLYGPPGTGKTLMARAVANETGAFFFLINGPEIMSKMAGESESNLRKAFEEAEKNSPAIIFIDEIDSIAPKREKTNGEVERRVVSQLLTLMDGMKARSNIVVMAATNRPNSIDPALRRFGRFDREVDIGIPDPTGRLEILQIHTKNMKLAEGVDLEQIAAETHGYVGSDVASLCSEAAMQQIREKMDLIDLDEDTIDAEVLDSLGVTMENFRFALGVSNPSALREVAVVEVPNVRWDDIGGLEEVKRELIESVQYPVEHPEKFLKFGMSPSRGVLFYGPPGTGKTLLAKAVANECAANFISVKGPELLSMWFGESESNIRDIFDKARAAAPCVVFLDELDSIAKSRGGSMGDAGGASDRVVNMLLTELDGMGVKKNVFVIGATNRPEQLDAALCRPGRLDTLVYVPLPNEASRADILRAQLRNTPCAPDIDLKYIASRTHGFSGADLGFITQRAVKLAIKQAISAEIEAQKERAERGEGADTEMVEAEGDDPVPELTKAHFEEAMAVARRSVSEVEIRRYEAFAQSMKQSGGMSSFRFPTAEEVGGGAESGFGQAGEDDDLYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLH2</accession>
    <name>D5GLH2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00010201001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430349">
        <property type="protein sequence ID" value="CAZ85365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841174.1">
        <property type="nucleotide sequence ID" value="XM_002841128.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLH2"/>
    <dbReference type="FunCoup" id="D5GLH2">
        <property type="interactions" value="9"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GLH2"/>
    <dbReference type="GeneID" id="9186109"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010201001"/>
    <dbReference type="eggNOG" id="KOG3839">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041093_1_1_1"/>
    <dbReference type="InParanoid" id="D5GLH2"/>
    <dbReference type="OMA" id="GCTADIR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005537">
        <property type="term" value="F:D-mannose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07308">
        <property type="entry name" value="lectin_leg-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000095">
        <property type="entry name" value="Lectin family integral membrane protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051136">
        <property type="entry name" value="Intracellular_Lectin-GPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005052">
        <property type="entry name" value="Lectin_leg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12223:SF45">
        <property type="entry name" value="RE50040P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12223">
        <property type="entry name" value="VESICULAR MANNOSE-BINDING LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03388">
        <property type="entry name" value="Lectin_leg-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51328">
        <property type="entry name" value="L_LECTIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072822" evidence="8">
        <location>
            <begin position="21"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="291"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="L-type lectin-like" evidence="9">
        <location>
            <begin position="30"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="259"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ85365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="324" mass="36254" checksum="B7062E0F6906F3FA" modified="2010-06-15" version="1">MKWSFCSIFTALALVREGLGQSTADDDVKTVPLRTHSLFQPYLDSELQSRWFDWGGDTIVRADQYIRLTSDRQQQAGWLWSRLPLTATNWEIEVEFKIHGQGNLYGDGMALWITKQRAMQGPVFGAADKFEGLGVFFDTYKNNRPGVVFPYVMAMLGDGQTVYQHGNDGKENELAGCSARGIRGAPIATKARLTYFQENYLQLDLQYKSEDQWTPCFNVPNVTLPQITYLGFTAETGELSDNHDIITVTAKNLYQVHTGTSGSSGKRPDKIGKAWKPSNGGGDRSESSGGWGWFFLKMIIFIAVIVGGYVGYTAYRAQNRASRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLK9</accession>
    <name>D5GLK9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00010253001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430349">
        <property type="protein sequence ID" value="CAZ85402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841211.1">
        <property type="nucleotide sequence ID" value="XM_002841165.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLK9"/>
    <dbReference type="STRING" id="656061.D5GLK9"/>
    <dbReference type="GeneID" id="9182705"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010253001"/>
    <dbReference type="eggNOG" id="KOG1158">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_17_5_1"/>
    <dbReference type="InParanoid" id="D5GLK9"/>
    <dbReference type="OMA" id="LCCGGGC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030586">
        <property type="term" value="F:[methionine synthase] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050667">
        <property type="term" value="P:homocysteine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000027">
        <property type="entry name" value="FAD binding domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.990.10:FF:000007">
        <property type="entry name" value="Methionine synthase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.990.10">
        <property type="entry name" value="NADPH-cytochrome p450 Reductase, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003097">
        <property type="entry name" value="CysJ-like_FAD-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023173">
        <property type="entry name" value="NADPH_Cyt_P450_Rdtase_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384:SF84">
        <property type="entry name" value="METHIONINE SYNTHASE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384">
        <property type="entry name" value="NITRIC OXIDE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00667">
        <property type="entry name" value="FAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding FR-type" evidence="6">
        <location>
            <begin position="141"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ85402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="552" mass="61305" checksum="FB6CF49198FBF35A" modified="2010-06-15" version="1">MKSPILGIDFTRTGRPRRASTHLLTVGPQNVCDVINDSSATNLVDSGCCGGGCSRLPPLPGNSVSLDTRSRTASEFVKAPDNAAFKSLNLRLSPLTSRDRLTGITALPAQTISIEPADEEEKHESTVYVHPPKYVTPHPPWAVYSAKIDSTRELTKTGAPKRTYHFDLDVTDYPEEISGVDFRVGGAIGVVAPNDFKLVAEVFDILGIDEDELDAPIQLSTQGGRWPTIWGKAKERALTTTLRELMTWTVDIQSYPPTKNLFRLLAEYAKDEAEKTILLYLCSRQGQAAFCELRTGPYISLIQLLNAFPSSKPPLDHLLCNLPTLMPRFYSLSSDPRESKSKDRRIIEIAVTIHEHEEGWRKGSRTGVGSGFFERCARRIIDEGADDIRIPMFRGLMANPLAKQFQADGPMLLIGAGVGIAPFRGFVQRRLQSANCANKVWVIQGVRDSLLDELYSGEWGEHEDKIRSVVQSRVGTGRYVQEEVYAQADLVWSIVNSLDGRVFVCGSSKGMGEGVHESLVKVVMKKSMLGREQAEEFWEQKKEDGQYIAETW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLY0</accession>
    <name>D5GLY0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00010468001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430351">
        <property type="protein sequence ID" value="CAZ85547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841356.1">
        <property type="nucleotide sequence ID" value="XM_002841310.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLY0"/>
    <dbReference type="STRING" id="656061.D5GLY0"/>
    <dbReference type="GeneID" id="9187327"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010468001"/>
    <dbReference type="eggNOG" id="ENOG502S3JY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_324441_0_0_1"/>
    <dbReference type="InParanoid" id="D5GLY0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="364"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="677"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="233"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="688"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="709"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="726"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="782"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="811"/>
            <end position="824"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="890" mass="100542" checksum="6250133B585AF9C5" modified="2010-06-15" version="1">MDPGFLDANIPTARRWFCTIDTRAKCHACGLPFDLVHVFDSSGEDTFPEGQKAAADEHRTFCPIIRVEGWSTDITEMRHPGDYRDQLVDALNRGDDIMSIMGEDHYDELIRPRLSRERIAAYQRYLNRKALAAQVMLKHCPKCLRASSTTLMGKRMHERVCQLTRFDWVTLAEVQPLRTQEGVDGREMVMNSNGSLESRFRVLAPGEKARLERKYQRQLYTRLNMDEKNLELLGDDNDSEDVPSDQDDNQIPRRHGPRRRRPHRRPIHSDNSDDENDYGPGRGRGRIASRRIPTLEEYNRWHEEVAPTILARNNDSTEQMLSRFQMEFQEMLRRNDPIAIGVPAPQPRPPLGTPLPANERKTLLARKTPRTREKNTATTGQSRPRIAPLRADSAGAGDVRAGPLRTNIRALSEADAERPIITEPNLALQRAYDRIPEGLISALEGTGLNDSRLGFGWRVHTLRLLSPAWNAQNARADALLKQKMEGKVVTARDTRPALQMYIDFFSTGGLITVGSRSLSFLEILCYPDATLETDQLFMQLLFPIDSAPRFNPSISLMPTGLRRAMATTPTLIGCFMLGLRRVMAFWGFEFRGTSSTGRPLWRLVPNFLPANHAWTRRVDHNHQRFTRLIRCLHLIGGSRGARSIYRCITRAIRERNLPVNERSQRLWRRTALGPLNMDPLAKAYPPDTDFRTDSEPEEAGEGRSHRPRSLSGSLSGSLNGSLNGSLNGGGDGRGGGAGSVGSPVDSQNLSWERAMDPAYNDNGDSEDDPHIADISSSSEGFLDPHERDVDFIPKRLPSSPIDTREVPAIQPQETQQTSESNRTPVGQRPRPRIERRYVPPPTRTARASDDFRHVERRIIPSEERARLEKLRAAALRESNPDITKVVKRGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLY5</accession>
    <name>D5GLY5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00010475001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430351">
        <property type="protein sequence ID" value="CAZ85552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841361.1">
        <property type="nucleotide sequence ID" value="XM_002841315.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLY5"/>
    <dbReference type="STRING" id="656061.D5GLY5"/>
    <dbReference type="GeneID" id="9187332"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010475001"/>
    <dbReference type="eggNOG" id="KOG0334">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1134254_0_0_1"/>
    <dbReference type="InParanoid" id="D5GLY5"/>
    <dbReference type="OMA" id="EQDRQTN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="245" mass="29290" checksum="8FA75E62909DDDB9" modified="2010-06-15" version="1">MTRYASRSRSPSPHAKRRRDDRYDPRDRRDYRDRRGDRPAGRRRDGYYDSWDRGRDRDDRDRDRDRARDRSRGGDRDRDRDRDRDRDRDRDRDRDRDRDRDRRRSRSRDRPRRAEKDRERDRDSRRDREDDRRGDSRVKEATPPPPAVTEEEKIRQRQERLEAWKRKRAEEEEAKKKASPAALISSLERQPVPGAVSPIVGSKAAVEQAPKSVVGIVPPSTHQTAFGLYEWLGIAYESTGICVWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GM46</accession>
    <name>D5GM46_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00010522001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430352">
        <property type="protein sequence ID" value="CAZ85589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841398.1">
        <property type="nucleotide sequence ID" value="XM_002841352.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GM46"/>
    <dbReference type="STRING" id="656061.D5GM46"/>
    <dbReference type="GeneID" id="9187373"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010522001"/>
    <dbReference type="eggNOG" id="KOG0226">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_29_0_1"/>
    <dbReference type="InParanoid" id="D5GM46"/>
    <dbReference type="OMA" id="FTAFQPR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12383">
        <property type="entry name" value="RRM_RBM42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050825">
        <property type="entry name" value="RBM42_RBP45_47-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034215">
        <property type="entry name" value="RBM42_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47640:SF11">
        <property type="entry name" value="RNA-BINDING PROTEIN 42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47640">
        <property type="entry name" value="TRNA SELENOCYSTEINE 1-ASSOCIATED PROTEIN 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="205"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="140"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="293"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="15"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="64"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="140"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="170"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ85589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="318" mass="34532" checksum="A76889142B262B81" modified="2010-06-15" version="1">MITSSLAEQETPPLSTSPPPPPTVETMSYPPPPGTSSSLPPRPPPPSIPQSTTPPAFSGFRPRQVAAPATAYAQQPQYNQPYSQQYPPAYQPPAQQYQAPTPSGAPHIVNPFPLPQQSFNRRADPRYADLDPELQAQIAQQQSIYDPSARASSNTTAAPVASKVEGSEFPAAPLNKPKNAPTVVRQGGGQTWTDSSLLEWDPSHFRLFVGNLAGEVTDDSLLKAFSKYPSVQKARVIRDKRTTKSKGYGFVAFSDGDEYFRAAREMNGKYIGSHPVLLKRSNTEIKPIVVGGKKGVKDGRVEKKKKKKKGGEQKNVLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>D5GME3</accession>
    <name>D5GME3_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">DNA 5'-3' helicase</fullName>
            <ecNumber evidence="17">5.6.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">GSTUM_00010670001</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the helicase family. RAD3/XPD subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.3" evidence="17"/>
    <dbReference type="EMBL" id="FN430354">
        <property type="protein sequence ID" value="CAZ85686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841495.1">
        <property type="nucleotide sequence ID" value="XM_002841449.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GME3"/>
    <dbReference type="FunCoup" id="D5GME3">
        <property type="interactions" value="791"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GME3"/>
    <dbReference type="GeneID" id="9183256"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010670001"/>
    <dbReference type="eggNOG" id="KOG1131">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011312_1_0_1"/>
    <dbReference type="InParanoid" id="D5GME3"/>
    <dbReference type="OMA" id="WQTMGIL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000112">
        <property type="term" value="C:nucleotide-excision repair factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045951">
        <property type="term" value="P:positive regulation of mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.275.40:FF:000001">
        <property type="entry name" value="DNA repair helicase (Rad3)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.20:FF:000001">
        <property type="entry name" value="DNA repair helicase rad15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000128">
        <property type="entry name" value="Putative DNA repair helicase RAD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.20">
        <property type="entry name" value="Bacterial XPD DNA helicase, FeS cluster domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006555">
        <property type="entry name" value="ATP-dep_Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045028">
        <property type="entry name" value="DinG/Rad3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010643">
        <property type="entry name" value="HBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014013">
        <property type="entry name" value="Helic_SF1/SF2_ATP-bd_DinG/Rad3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006554">
        <property type="entry name" value="Helicase-like_DEXD_c2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010614">
        <property type="entry name" value="RAD3-like_helicase_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013020">
        <property type="entry name" value="Rad3/Chl1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001945">
        <property type="entry name" value="RAD3/XPD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042493">
        <property type="entry name" value="XPD_DNA_FeS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00604">
        <property type="entry name" value="rad3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472">
        <property type="entry name" value="DNA REPAIR DEAD HELICASE RAD3/XP-D SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472:SF1">
        <property type="entry name" value="GENERAL TRANSCRIPTION AND DNA REPAIR FACTOR IIH HELICASE SUBUNIT XPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06733">
        <property type="entry name" value="DEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06777">
        <property type="entry name" value="HBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13307">
        <property type="entry name" value="Helicase_C_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00852">
        <property type="entry name" value="XRODRMPGMNTD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00488">
        <property type="entry name" value="DEXDc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00491">
        <property type="entry name" value="HELICc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51193">
        <property type="entry name" value="HELICASE_ATP_BIND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0347">Helicase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="15" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="21">
        <location>
            <begin position="49"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="722"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="268"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="20">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="731"/>
            <end position="741"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00044969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="CAZ85686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="748" mass="85004" checksum="795C709CD9D0D477" modified="2010-06-15" version="1">MFAKSFKRVSRPKPHVEAQSFKRKKKKRPTSPLHRAAPPQLAMRFKIDELEVLFPYPRIYPGEWRYMTDLKRTLDAGGHCVLEMPSGTGKTVSLLSLIVAYQQSFPEPRKLIYCSRTMSEIEKALAELKALMAYRSKELGYVEEFRGSLTAGFVKEKKARGEEVEVCVFHDNLDTLEPHNLITPGVYTLDGMLKYGEKHTQCPYFTARRMMPFCNVIIYSYHYLLDPKIAERVSKELSKDCIVVFDEAHNIDNVCIESLSIDLTDDSLRRAARGAQNLEKKINEMKASDAEKLQNEYKKLVEGLRDVDEGTEEDSFMANPVLPKDLLKEAIPGNIRRAEHFLAFLKRFVEYLKTRMKVLHVISETPASFLQHLKDLTFIEKKPLRFCAERLTSLVRTLELANIEDYKSLQEVAMFATLVATYEKGFLLILEPFESETATVPNPILHFTCLDASIAIRPVFERFSSVVITSGTISPLEMYPKMLQFNTVVQESYSMTLARRSFLPMIVTRGSDQVAVSTRFEIRNDPSVVRNYGSLLVEFSKITPDGMVVFFPSYLYMESIISMWQGMGILDEVWKYKLILVETPDSQETSLALETYRTACCNGRGAISTTISVELCFESPSNTPKNDFLSFDAMRHAAQCLGRVLRGKDDYGIMVLADRRFGKKRSQLPKWINQALLESDVNLSTDMAVAIAKKFLRTMAQPFAPKDQEGISTWSLADLEEHQGKGSELQDVGEEEGEEDVVMANAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMG4</accession>
    <name>D5GMG4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="13">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00010693001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the TRAFAC class translation factor GTPase superfamily. Bms1-like GTPase family. BMS1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430354">
        <property type="protein sequence ID" value="CAZ85707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841516.1">
        <property type="nucleotide sequence ID" value="XM_002841470.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMG4"/>
    <dbReference type="FunCoup" id="D5GMG4">
        <property type="interactions" value="1355"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GMG4"/>
    <dbReference type="GeneID" id="9182133"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010693001"/>
    <dbReference type="eggNOG" id="KOG1951">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002486_0_0_1"/>
    <dbReference type="InParanoid" id="D5GMG4"/>
    <dbReference type="OMA" id="KLHVPMV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000232">
        <property type="term" value="P:regulation of rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01882">
        <property type="entry name" value="BMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000105">
        <property type="entry name" value="BMS1 ribosome biogenesis factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012948">
        <property type="entry name" value="AARP2CN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039761">
        <property type="entry name" value="Bms1/Tsr1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037875">
        <property type="entry name" value="Bms1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007034">
        <property type="entry name" value="BMS1_TSR1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030387">
        <property type="entry name" value="G_Bms1/Tsr1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12858">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12858:SF2">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BMS1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08142">
        <property type="entry name" value="AARP2CN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04950">
        <property type="entry name" value="RIBIOP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00785">
        <property type="entry name" value="AARP2CN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01362">
        <property type="entry name" value="DUF663"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51714">
        <property type="entry name" value="G_BMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Bms1-type G" evidence="12">
        <location>
            <begin position="61"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="294"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="389"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="463"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="551"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="639"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1114"/>
            <end position="1179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="422"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="483"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="493"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="511"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="590"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="653"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="691"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1114"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="1170"/>
            <end position="1179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00061391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ85707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1179" mass="132911" checksum="F42D797C4EF78DA2" modified="2010-06-15" version="1">MDQSNKTHRKPKEKKKFTGQNPKAFAFANPGKLQKQATRSHDVREKRLHVPMVDRLPEEPPPLIVAVVGPPGVGKTTLVKSLVKRYTKHTLSSIQGPITVVTSKKRRLTFLECDNSLTSMIDISKIADIVLLLIDGNFGFEMETMEFLNVIATHGLPSNIFGILTHLDLFRSQSLLRTTKKRLKHRFWSELYQGAKLFYLSGVINGRYPDREIHNLSRFISVMKNPRPLVWRNSHYYMLADRMVDLTEPVAIEDDPKADRKVALYGYVRGLSFPNEGARVHIPGVGDLSVSSVESLPDPCPTPARTASEGKGRKRLGEKQKLIYAPMSDVGGVLVDKDAVYIDVPSQNFEEGVEAQGVGERLVIGLQKGRRGEVDGVRLFTGGEVLKQVQDDGEENGDVGRKHKRSVRALQSSKEDTGVEGYSDEDEDEEMGDADIASGSEDDDASDNGGITLKSFKLGKALRGDQDQGDAGDDITFAESDSDLGSVSVVSDQEFDEDDLPSDEDEDDDDVPRWKQDLSDNAKQLWTKSHRTVNLSKLIYDESLTPTEVIQKWRGMQGQKSQEKPKNTSDGSDSEEDFFKKVEEEVDDEHDSKPPSKKIADRGLPVYDMEKLENYWSEEFALEDLRRRFITANLLEEKERAEGEDGEFAGIDDSGDEGDGAFEDLETGEVHGGGVDEGGRNESKEDGEETSLEKEREKNARRKEELRLRFEEEDREGVNNDRTGGGRDGGGFGEDDWYDAQKAKFAKQLAVNQSEFEQLDDATRVKVEGHRAGTYARVVLSNVPYEFVAHFDPKYPILVGGLTATEDRFGFLQVRIKRHRWHKKILKTNDPLIFSLGWRRFQSIPIYSTSDSRTRNRMLKYTPEHMHCFGTFYGPLIAPNTGFCAVQCLSSKNPGFRVSATGVVLSVDQSTEIVKKLKLTGTPYKIFKNTAFIKDMFNSSLEVAKFEGASIRTVSGVRGQIKRALANAKQDGHFRATFEDKVLMSDIIFLRAWYPIKPRKFYNPVTNLLADTTLPDAPAWNGMRTTGEVRRELNIKTPTIADSTYKPIERSTRRFNPLRVPKAIQKDLPFASQIHQMKPRKRATYLASRAVVVSSEERKARDLVNKIATLKKEKEAKRREAKEKKREVYRKKVAENTEMKAAREKRERDEYWAKEGKKRRNTENAGGGGRGKRRRGGGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMN6</accession>
    <name>D5GMN6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00010832001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430357">
        <property type="protein sequence ID" value="CAZ85779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841588.1">
        <property type="nucleotide sequence ID" value="XM_002841542.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMN6"/>
    <dbReference type="GeneID" id="9185115"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010832001"/>
    <dbReference type="HOGENOM" id="CLU_1929127_0_0_1"/>
    <dbReference type="InParanoid" id="D5GMN6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ85779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="131" mass="15208" checksum="05AD5D24874B2DB5" modified="2010-06-15" version="1">MLTTLLVLAYTFQKDPETLALPRPKPCPSFSMPLEGASTSGYYRYTQSRSLFFLSFRSPGSVSLFYLYRYRTSNPLALVLIIWLDIVTIPGTRHRTHDRVSDQPLPICPWKWPDPSHHLSQPFQVPWFFNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>D5GN32</accession>
    <name>D5GN32_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00011065001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430362">
        <property type="protein sequence ID" value="CAZ85925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GN32"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011065001"/>
    <dbReference type="HOGENOM" id="CLU_2514273_0_0_1"/>
    <dbReference type="InParanoid" id="D5GN32"/>
    <dbReference type="OMA" id="IRDMQEC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="85" mass="8864" checksum="04DB14E6F72AE78C" modified="2010-06-15" version="1">MSSSSSSNTNNESTLLVPNPTPTSTSRRGSIPRNTPAWTGVGDASGSGNPEDPASSLRKEMNLVAEAAKRASVAIVVRDMQEFSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5GN85</accession>
    <name>D5GN85_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Selenoprotein O</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00011155001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SELO family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430362">
        <property type="protein sequence ID" value="CAZ85978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841787.1">
        <property type="nucleotide sequence ID" value="XM_002841741.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GN85"/>
    <dbReference type="FunCoup" id="D5GN85">
        <property type="interactions" value="309"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GN85"/>
    <dbReference type="GeneID" id="9185904"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011155001"/>
    <dbReference type="eggNOG" id="KOG2542">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010245_2_1_1"/>
    <dbReference type="InParanoid" id="D5GN85"/>
    <dbReference type="OMA" id="YGPYGWL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070733">
        <property type="term" value="F:AMPylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00692">
        <property type="entry name" value="SelO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003846">
        <property type="entry name" value="SelO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32057">
        <property type="entry name" value="PROTEIN ADENYLYLTRANSFERASE SELO, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32057:SF14">
        <property type="entry name" value="PROTEIN ADENYLYLTRANSFERASE SELO, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02696">
        <property type="entry name" value="SelO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="39"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ85978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="568" mass="64252" checksum="601775E475E8C8A2" modified="2010-06-15" version="1">MPRSSLAMSRSESTGVTLQDLPKSNVFTTKLPPDAQFPTPESSAGATRSQLGPRMVKAALYTYVRPDPVEDNPELLAVSPLALRSIGLASTEPTKPEFLRLVSGNGGFEDISYPWAQCYGGWQFGQWAGQLGDGRAISLFEATNPETKIRYELQLKGAGQTPYSRFADGKAVLRSSIREFIVSEYLYSIGIPSTRALSLTLLPGNQAIRENIETCAIVCRFAESWIRIGTFDLLRARGDRKNLRLLSDYVREEVLKTKERVDGEDGSSGVRGDGVRNRYEDMYREIVRRNALTVAKWQAYGFMNGVLNTDNTSIMGLSLDFGPFSFMDSFNPKFTPNHDDHTLRYCYKNQPTIIWWNLVRLAEDLAELFAATPEMLDSEVGEEYKSIFLAEYKQLMATRLGFTGLRETDMDDVYSPLLDILEDAHVDFGHFFRRLSELPIFELMESDEEAQLTAAEGLMPKAVLTTVQKGPEKILKWLKLYAERLEEKEDAKRMERMKKVNPKFIPKNWVLEEIIQRVEQKGERGVLGDVIKLVENPFADRWEGVKDAERWCGDVPKLEVGMQCSCSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNQ2</accession>
    <name>D5GNQ2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 3 subunit H</fullName>
            <shortName evidence="4">eIF3h</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00011424001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the eIF-3 subunit H family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430367">
        <property type="protein sequence ID" value="CAZ86149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841958.1">
        <property type="nucleotide sequence ID" value="XM_002841912.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNQ2"/>
    <dbReference type="STRING" id="656061.D5GNQ2"/>
    <dbReference type="GeneID" id="9182784"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011424001"/>
    <dbReference type="eggNOG" id="KOG1560">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044094_1_0_1"/>
    <dbReference type="InParanoid" id="D5GNQ2"/>
    <dbReference type="OMA" id="WYQSTYF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd08065">
        <property type="entry name" value="MPN_eIF3h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000052">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 subunit H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03007">
        <property type="entry name" value="eIF3h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027524">
        <property type="entry name" value="eIF3h"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045810">
        <property type="entry name" value="eIF3h_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050242">
        <property type="entry name" value="JAMM_MPN+_peptidase_M67A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10410">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19445">
        <property type="entry name" value="eIF3h_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01398">
        <property type="entry name" value="JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MPN" evidence="6">
        <location>
            <begin position="18"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ86149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="366" mass="40880" checksum="BEF7D2E9A1FC6E5F" modified="2010-06-15" version="1">MADKSKIVLPASGPLKSVQVDALVVLKICKHASSAHPQVITGQLLGMDVDGELQVTNSFPFPSTDPSTSSNDPDSNLASAAALAPRSKASAWYQTEMVRCLREMNVDANSVGWYQSANLGNFLNTNFIDNQYFYQNALNERTVALVHDTARSAQGALSLRAFRLTPQFMAVQKEGKFTSESLAKHNLTYRDILIELPVSVHNSHLLTTFLHQLDTPPIPNPFNPFSQPSSTPLFPNFDSLDLSIDPFLEKNCDLLLDSIETHYTELNNSQYYQRQLAREQTKIAAWLTKRKQENILRAAAKQPLLPENEWTRLFKPPVEPSRLETLLNSRQIEQYAKQVDGFTASVTAKMFAVNGGLSKTQEQLQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNS1</accession>
    <name>D5GNS1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00011449001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the band 7/mec-2 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430367">
        <property type="protein sequence ID" value="CAZ86168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841977.1">
        <property type="nucleotide sequence ID" value="XM_002841931.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNS1"/>
    <dbReference type="STRING" id="656061.D5GNS1"/>
    <dbReference type="GeneID" id="9182803"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011449001"/>
    <dbReference type="eggNOG" id="KOG2620">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024949_1_0_1"/>
    <dbReference type="InParanoid" id="D5GNS1"/>
    <dbReference type="OMA" id="YLQMLPK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08829">
        <property type="entry name" value="SPFH_paraslipin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.479.30:FF:000004">
        <property type="entry name" value="Putative membrane protease family, stomatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="entry name" value="Band 7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050710">
        <property type="entry name" value="Band7/mec-2_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032435">
        <property type="entry name" value="STML2-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001972">
        <property type="entry name" value="Stomatin_HflK_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43327">
        <property type="entry name" value="STOMATIN-LIKE PROTEIN 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43327:SF10">
        <property type="entry name" value="STOMATIN-LIKE PROTEIN 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16200">
        <property type="entry name" value="Band_7_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00721">
        <property type="entry name" value="STOMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="Band 7/SPFH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Band 7" evidence="4">
        <location>
            <begin position="91"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ86168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="400" mass="43023" checksum="9500E9612E199C1E" modified="2010-06-15" version="1">MSSAIFRSSSPLVQIIARQPSTAIAYRSISILTRPLTPGSLQTTQVLGQKKNISSSTATLAPPQNQPIFSAPPGHGPSGGFFRKESLPANTIIRFVPQQTAWIVERMGKFHRILDPGLAILWPIIDKIKYVKSLKEAAIEIPSQSAITADNVTLEMDGVLYIRVFDAYKASYGVEDAEFAISQLAQTTMRSEIGQLTLDHVLKERAALNINITHAINEASAEWGLVCLRYEIRDIHAPNPVLQAMHRMVSAERSKRAEILESEGQRQSAINVAEGKKQSVILASEAKKAEQINFAAGEAQAILMKADATARGIEAVAKAIRENKEAAQGAVSLSVAEKYVEAFGQLAKQSNTVVVPAQLGDLGGMIAGAMGIYGKVAETQASQQKMLGAQLEGVKAGRGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNW1</accession>
    <name>D5GNW1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Carbonic anhydrase</fullName>
            <ecNumber evidence="4 9">4.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00011540001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 9">Reversible hydration of carbon dioxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>hydrogencarbonate + H(+) = CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:10748"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="EC" id="4.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the alpha-carbonic anhydrase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.1" evidence="4 9"/>
    <dbReference type="EMBL" id="FN430370">
        <property type="protein sequence ID" value="CAZ86204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842013.1">
        <property type="nucleotide sequence ID" value="XM_002841967.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNW1"/>
    <dbReference type="STRING" id="656061.D5GNW1"/>
    <dbReference type="GeneID" id="9182838"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011540001"/>
    <dbReference type="eggNOG" id="KOG0382">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039326_0_1_1"/>
    <dbReference type="InParanoid" id="D5GNW1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03124">
        <property type="entry name" value="alpha_CA_prokaryotic_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.200.10">
        <property type="entry name" value="Alpha carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041891">
        <property type="entry name" value="Alpha_CA_prokaryot-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001148">
        <property type="entry name" value="CA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036398">
        <property type="entry name" value="CA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023561">
        <property type="entry name" value="Carbonic_anhydrase_a-class"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018338">
        <property type="entry name" value="Carbonic_anhydrase_a-class_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952:SF265">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00194">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01057">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51069">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00162">
        <property type="entry name" value="ALPHA_CA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51144">
        <property type="entry name" value="ALPHA_CA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="6 9" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025099194" description="Carbonic anhydrase" evidence="9">
        <location>
            <begin position="23"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-carbonic anhydrase" evidence="10">
        <location>
            <begin position="46"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ86204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="298" mass="33554" checksum="3F374CC784789D9F" modified="2010-06-15" version="1">MHSPSTTTHLLLLTFLLTLTHALLNTQTPLLQPETSSPNNLTIATAPWGYHYHSGPLSWHRLSPANVLCKTGIRQSPINIHQALKRPTGLKLRRGDYREYGILENEGQTVSIKPQEEDAKYGYQLYPGDSAGGGGGVGKGGGWALLDTMHFHTPSEHHLDGEHFVAELHAVFKTTDGQTTVLSTLLDIDTTSSNHLLTTLHPWLPFVATPLTATFVQFDPSPILDRFYRAYRRERVYTYRGSLTTPPCTENVLRMVSQDPEKISVEHYRRLKAITKFNARYPQNHPGYMNLLEWACEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNX4</accession>
    <name>D5GNX4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00011570001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MOG1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430371">
        <property type="protein sequence ID" value="CAZ86217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842026.1">
        <property type="nucleotide sequence ID" value="XM_002841980.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNX4"/>
    <dbReference type="FunCoup" id="D5GNX4">
        <property type="interactions" value="219"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GNX4"/>
    <dbReference type="GeneID" id="9183510"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011570001"/>
    <dbReference type="eggNOG" id="KOG3329">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081345_1_2_1"/>
    <dbReference type="InParanoid" id="D5GNX4"/>
    <dbReference type="OMA" id="IDTVKVW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1000.10">
        <property type="entry name" value="Mog1/PsbP, alpha/beta/alpha sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007681">
        <property type="entry name" value="Mog1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016123">
        <property type="entry name" value="Mog1/PsbP_a/b/a-sand"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15837">
        <property type="entry name" value="RAN GUANINE NUCLEOTIDE RELEASE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15837:SF0">
        <property type="entry name" value="RAN GUANINE NUCLEOTIDE RELEASE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04603">
        <property type="entry name" value="Mog1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55724">
        <property type="entry name" value="Mog1p/PsbP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ86217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="221" mass="24410" checksum="83816103AF8375CF" modified="2010-06-15" version="1">MSSLSFTPVALFGGAIRATVPLSFRDAADFRQVPDTQEVYVSMEEGMDISVVIDLNQRVQAPSDLEALDIHFQDVADDQDRSYAAVSRETITLPKMADKPAYMIIGTISSRPSDNPEYFIGILMTLVRLPQQTTDILITVNIPFNTSELAQAEKYLVSPRAETIGGGGEKSQMLRLGMAVLEGVLREFEVLDWGLFLEDVPEEGEWEGMRGDTEMEGVRAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPG6</accession>
    <name>D5GPG6_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">NEDD8-conjugating enzyme UBC12</fullName>
            <ecNumber evidence="11">2.3.2.34</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">E2 ubiquitin-conjugating enzyme 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">NEDD8-conjugating enzyme Ubc12</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">RUB1-conjugating enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Ubiquitin carrier protein 12</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Ubiquitin carrier protein UBC2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Ubiquitin-conjugating enzyme E2 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Ubiquitin-protein ligase UBC2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">GSTUM_00011837001</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine + [E2 NEDD8-conjugating enzyme]-L-cysteine = [E1 NEDD8-activating enzyme]-L-cysteine + [E2 NEDD8-conjugating enzyme]-S-[NEDD8-protein]-yl-L-cysteine.</text>
            <dbReference type="EC" id="2.3.2.34"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein neddylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.34" evidence="11"/>
    <dbReference type="EMBL" id="FN430375">
        <property type="protein sequence ID" value="CAZ86409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842218.1">
        <property type="nucleotide sequence ID" value="XM_002842172.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPG6"/>
    <dbReference type="FunCoup" id="D5GPG6">
        <property type="interactions" value="791"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GPG6"/>
    <dbReference type="GeneID" id="9186649"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011837001"/>
    <dbReference type="eggNOG" id="KOG0420">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030988_6_0_1"/>
    <dbReference type="InParanoid" id="D5GPG6"/>
    <dbReference type="OMA" id="CQVDFPD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061654">
        <property type="term" value="F:NEDD8 conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd23794">
        <property type="entry name" value="UBCc_UBE2F_UBE2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000005">
        <property type="entry name" value="NEDD8-conjugating enzyme Ubc12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 17" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="18">
        <location>
            <begin position="28"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="16">
        <location>
            <position position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00041569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00042179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00042190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00043698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00044315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAZ86409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="182" mass="20742" checksum="67AAA75A49BBCA72" modified="2010-06-15" version="1">MLKIWSMKQQQQAASAAEGGQKKKKVTAAQLRAQKDLSELSLPPTIRMHMPDPEDILNFTLTIEPDEGMYKCGSFNFTFAINSNFPHEPPKVKCTQKIYHPNIDLDGNVCLNILREDWKPVLNLNAVIVGLQFLFLEPNPSDPLNKEAAEDLRLTRENFKRNVKNSMNGGYVKNVQFERVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPK0</accession>
    <name>D5GPK0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00011880001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430375">
        <property type="protein sequence ID" value="CAZ86443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842252.1">
        <property type="nucleotide sequence ID" value="XM_002842206.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPK0"/>
    <dbReference type="GeneID" id="9186683"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011880001"/>
    <dbReference type="eggNOG" id="ENOG502R9XT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_821809_0_0_1"/>
    <dbReference type="InParanoid" id="D5GPK0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="222"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="238"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="262"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ86443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="338" mass="37603" checksum="CA98D8F8A13B7F70" modified="2010-06-15" version="1">MVVDELDPPPKFDTLALLFSHYTVVTELAKMITTGDLIQLSRTSSTIRETLLQSRAHWERLVSLTSLDCSERDHIKGEKIKGCRICSMPVCETCIVRADFFGQNGTYSNRIRTFCDTCWSATDFSDTDLTPQDLVNYAAPVLCECVAKDGWLCSSCRAIERSELVYNKRVCATDGCDNPHNSSEKHRVCTWCQHPLPAAWRPTGEPNVLGRRIFNEDGTVRVDSADDSLEDGGSASPMEDRGLRRAKSTDKIAGWDQEDEDSKARYKEAWLKKGARERERDREHQSESSGSSQREGRKAFVIASEVGDDDGAGQKEEQGSSDDEPSEGMGNPPPYSGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5GQ40</accession>
    <name>D5GQ40_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00012188001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430382">
        <property type="protein sequence ID" value="CAZ86633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842442.1">
        <property type="nucleotide sequence ID" value="XM_002842396.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GQ40"/>
    <dbReference type="FunCoup" id="D5GQ40">
        <property type="interactions" value="98"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GQ40"/>
    <dbReference type="GeneID" id="9185213"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012188001"/>
    <dbReference type="eggNOG" id="KOG2845">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027500_1_0_1"/>
    <dbReference type="InParanoid" id="D5GQ40"/>
    <dbReference type="OMA" id="MWASPQE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0180022">
        <property type="term" value="C:RQC-trigger complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039128">
        <property type="entry name" value="TRIP4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009349">
        <property type="entry name" value="TRIP4/RQT4_C2HC5_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12963:SF4">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12963">
        <property type="entry name" value="THYROID RECEPTOR INTERACTING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06221">
        <property type="entry name" value="zf-C2HC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TRIP4/RQT4 C2HC5-type zinc finger" evidence="2">
        <location>
            <begin position="246"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="451"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="76"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="142"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ86633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="550" mass="59702" checksum="6576E6C721D9185D" modified="2010-06-15" version="1">MSSKSSTESWALTRLESLLPLPEDSLRDVITYALSLTDPSGVSSHFEGLLGSTEEVLDFISDFNSRLFPQRATRESTTLQSSSSTSSLNAPPAKRRGKKGKLPINRYANRPRQVEGQFGNPAAVYMKKDVEDEYYTGGSKGPTSKPVDSPSPSSSVPVSRTATPAPRSASPQRAQQKAQQSSNSNIGGKLVSDLLNVKTKVKKAPQKVHVTGGVAMRGASAELTDLESALRALELSTNPTLTSKSQKCNCAGMKHEVLSAAPNCLSCGKIICIKEGLAPCSFCNTPLIPPEELQSMVRELREESGRERMAINNATQKRPEVVHAPKPFSSYDSTPKTNDEGLKKATEHRDRLLGFQATSAQRTKIIDQAADFETPAMGSSMWATPQERALQLKKQQKAMREMEWRAKEDYEKRQVVVAIDLKGRQAVRKMQEIERPREDSDDGDIEEAYDDTLERGGTAAATDSSTSKGGAYSRNPLLKGMIKPVYDSGKGKGKGKQGDEIVDARPGAYAGGSRGWRRVQDDFADNEGVTLDGGVGREERGTQGEEPACG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5G3Z8</accession>
    <name>D5G3Z8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Kinetochore protein SPC25</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00003881001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the NDC80 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Chromosome</location>
            <location evidence="9">Centromere</location>
            <location evidence="9">Kinetochore</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the SPC25 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835120.1">
        <property type="nucleotide sequence ID" value="XM_002835074.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G3Z8"/>
    <dbReference type="STRING" id="656061.D5G3Z8"/>
    <dbReference type="GeneID" id="9182368"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003881001"/>
    <dbReference type="eggNOG" id="KOG4657">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065188_0_0_1"/>
    <dbReference type="InParanoid" id="D5G3Z8"/>
    <dbReference type="OMA" id="HEDQRMK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031262">
        <property type="term" value="C:Ndc80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23784">
        <property type="entry name" value="RWD_Spc25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.457.50:FF:000001">
        <property type="entry name" value="Probable kinetochore protein spc25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.457.50">
        <property type="entry name" value="Chromosome segregation protein Spc25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045143">
        <property type="entry name" value="Spc25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013255">
        <property type="entry name" value="Spc25_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14281:SF0">
        <property type="entry name" value="KINETOCHORE PROTEIN SPC25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14281">
        <property type="entry name" value="KINETOCHORE PROTEIN SPC25-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08234">
        <property type="entry name" value="Spindle_Spc25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 9" id="KW-0132">Cell division</keyword>
    <keyword evidence="8 9" id="KW-0137">Centromere</keyword>
    <keyword evidence="2 9" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 9" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="4 9" id="KW-0498">Mitosis</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromosome segregation protein Spc25 C-terminal" evidence="12">
        <location>
            <begin position="187"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="90"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ79241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="265" mass="30877" checksum="60EA05F5803A39EC" modified="2010-06-15" version="1">MSFNLASSSAPPSSQTHALSSPQATNHSMAATLPQINFGFEELKDRMARFTVRFDDFIEKGRRRILEEKNEFAKTIVEDKDTQRILKKEIEHYKQKEKDVSENVAKEQLEATEAEHTIAEMDRKKQSRLEHKAHLVAQIEATQEAIRKKREIRAEERKALSFQSSRNAPELAFWEDHLGMRIEGAGVTDHLRIVYTHIVDSDWSKEFSFIINMVTRDYEVIQCRPKLDSALVGKWVDRLNETRDLTCFLKEMRQLFKEYSSSRGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5G436</accession>
    <name>D5G436_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00003933001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835158.1">
        <property type="nucleotide sequence ID" value="XM_002835112.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G436"/>
    <dbReference type="GeneID" id="9182406"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003933001"/>
    <dbReference type="eggNOG" id="ENOG502S7VK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062858_0_0_1"/>
    <dbReference type="InParanoid" id="D5G436"/>
    <dbReference type="OMA" id="HYSYECT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039715">
        <property type="entry name" value="ZCCHC10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13491">
        <property type="entry name" value="ZCCHC10 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13491:SF0">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13917">
        <property type="entry name" value="zf-CCHC_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="118"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="144"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="171"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="203"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="218"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="263" mass="30337" checksum="62ACE4171E7E7C24" modified="2010-06-15" version="1">MNRYRGPFSSSAASSKATPTTRCQKCLKLGHYSYECKATISERPYTSRPSRTQQFLNPRLRQELTEAAPPSDVTVNRKKGTADEILMKNKNERDSAHSPEVRGRKRRRNSTSPSPVQSRARSISTGSSSTYSSISSGRSPTPPVYSQENSVVRHNSRRQSNSHARDDHDRSVRRKYRDSPSPRRRGRRLSPTDYPRRTRSRTPRPTYRRSITPQVYNGRKESPVKCRQVDENRPTRTSPYRERSLSPFTRRKLLTEQMQRVPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5G461</accession>
    <name>D5G461_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00003964001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="CAZ79304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835183.1">
        <property type="nucleotide sequence ID" value="XM_002835137.1"/>
    </dbReference>
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    <dbReference type="KEGG" id="tml:GSTUM_00003964001"/>
    <dbReference type="HOGENOM" id="CLU_3320327_0_0_1"/>
    <dbReference type="InParanoid" id="D5G461"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="39" mass="4330" checksum="10B6D79BC00B405A" modified="2010-06-15" version="1">MKIPNPGHLFTAPPLSVFILYFLHFVIVLLILGYSVMGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4P0</accession>
    <name>D5G4P0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00000086001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN429990">
        <property type="protein sequence ID" value="CAZ79516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835359.1">
        <property type="nucleotide sequence ID" value="XM_002835313.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4P0"/>
    <dbReference type="FunCoup" id="D5G4P0">
        <property type="interactions" value="1341"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G4P0"/>
    <dbReference type="GeneID" id="9182997"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000086001"/>
    <dbReference type="eggNOG" id="KOG0301">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011791_2_0_1"/>
    <dbReference type="InParanoid" id="D5G4P0"/>
    <dbReference type="OMA" id="DKCIYYW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010992">
        <property type="term" value="P:ubiquitin recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000236">
        <property type="entry name" value="Polyubiquitin binding protein (Doa1/Ufd3)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.870">
        <property type="entry name" value="PFU (PLAA family ubiquitin binding), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015155">
        <property type="entry name" value="PFU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038122">
        <property type="entry name" value="PFU_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013535">
        <property type="entry name" value="PUL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19849">
        <property type="entry name" value="PHOSPHOLIPASE A-2-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19849:SF0">
        <property type="entry name" value="PHOSPHOLIPASE A-2-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09070">
        <property type="entry name" value="PFU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08324">
        <property type="entry name" value="PUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51394">
        <property type="entry name" value="PFU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51396">
        <property type="entry name" value="PUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="11"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="106"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="144"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="183"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="223"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="PFU" evidence="6">
        <location>
            <begin position="353"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="PUL" evidence="7">
        <location>
            <begin position="474"/>
            <end position="750"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ79516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="751" mass="81923" checksum="9F37623577E1A0A3" modified="2010-06-15" version="1">MASIYKLTAALRGHEDDVRGVVFPSPYFVTSVSRDATVRLWKREDSDGTVFSDHINSTGSGFINSVTFILPTEEHPKGLVVSGGQDTIIEVREPGSKDTDDAKYLLLGHTHNVCALDNYAGIIISGSWDGTARVWKNWESQYVLDGHDQGVLAVLVLSGTDVVTGSADKTIRIWRNGKTIKKIEGHTDCVRGLCRMPNGGFASCANDGTVRMWSSDGHQLQELHGHTAFIYSVATLPSGELVSAGEDRTVRIWRDGDCVQTITHPAISVWSVAVCAQNGDIVSGASDRIVRVFSREEKRWADEGALKVILIIENQVGDVKKDDLPGLEALRQPGRKDGQVLMVRNGETVEAHVWSNAARAWNNVGTVVDAVGSSRKQMFEGKEYDYVFDVDIQEGAPALKLPYNASENPFEAARRFLEANELPISYLDTVGQFIVKNAGGVALGVQEQPAGPDPWGMENRYRPDAPTPQQAKPKRIPQKSYLSITAASLPTIQNKINELNRELLDKGEKDIALNPDETSALAKLCGSLQAVASPSYKAGTHNASFSNGLELISKIITSWHPQSRLPGLDLLRLVAAATPLAATYEPMNGLKAGDILETSGAFDISHPNNVMLATRTFVNLFQTEEGRGYMDNRFERTIQLAEKASAGTANRNLKIAKATLLLNYAVLITSEGAIDRAITLLEPLTNIVKKEIDSETTFRALVGLGTLLTLQKEVKEAALSVYETRQTVAEAEIRVKEPRIKGLATEIRGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5A3</accession>
    <name>D5G5A3_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Vacuolar calcium ion transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00004245001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Has a role in promoting intracellular calcium ion sequestration via the exchange of calcium ions for hydrogen ions across the vacuolar membrane. Involved also in manganese ion homeostasis via its uptake into the vacuole.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Vacuole membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835539.1">
        <property type="nucleotide sequence ID" value="XM_002835493.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5A3"/>
    <dbReference type="GeneID" id="9188115"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004245001"/>
    <dbReference type="eggNOG" id="KOG1397">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008721_4_0_1"/>
    <dbReference type="InParanoid" id="D5G5A3"/>
    <dbReference type="OMA" id="WNPFRHV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015369">
        <property type="term" value="F:calcium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.30:FF:000011">
        <property type="entry name" value="Vacuolar calcium ion transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004713">
        <property type="entry name" value="CaH_exchang"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004798">
        <property type="entry name" value="CAX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00846">
        <property type="entry name" value="caca2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00378">
        <property type="entry name" value="cax"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31503:SF20">
        <property type="entry name" value="CA(2+)_H(+) EXCHANGER, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31503">
        <property type="entry name" value="VACUOLAR CALCIUM ION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0050">Antiport</keyword>
    <keyword evidence="6 10" id="KW-0106">Calcium</keyword>
    <keyword evidence="4 10" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="8 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <keyword evidence="10" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="213"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="241"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="271"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="300"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="336"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="371"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="418"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="448"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="489"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="521"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="554"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="12">
        <location>
            <begin position="240"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="12">
        <location>
            <begin position="423"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="44"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="128"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="60"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ79696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="580" mass="63232" checksum="BA7538EC46314856" modified="2010-06-15" version="1">MTRGEYRDQSKILVGHTPVPTFLGVLYPRSTIPRGHEILHLFPFISSNPPPPPRTRGSKKLSSCQPSSSPRPCTFQNSKSIPYHCTRFATGSQVVMHRLNRAASCEADTEGFNPFCRLRQHRRRKALSRLRNRYSSQDGPNKDRERVATARSPPTAEESFGSQATVVPEGPNRVDTITMEEGRPQMANIQRMSHYSEKEMEEHKTIWGQFKAAIFNSWINVLLVFAPVGIATHYAGVDPTIVFVMNFVAIIPLAALLSYGTEEIALYCGDVLGGLLNASFGNAVELIISILALVKGEIVIVQTSLIGSILSNLLLVLGLSFIAGGYNRIQQSFNTTVAQTASSLLALAVGSLVVPTAFHMAGPSNTGITELSRGTAVILLLVYASYLLFQLKTHSDIYNEPSEKSPKRNHEEREEPDLTLWAAVILLAVSTALVAICAECLVDSIDHLVTSAGISRVFVGLILLPIVGNAAEHATAVTVAIKDKMDLSIGVAVGSSMQIALLVIPFIVVLGWIIGEDEMTLYFDGFQVMILFVSVLLVNYLIQDGKSNYLEGNLLVALYAIVSLASFFYPEEGPLAQVSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5E1</accession>
    <name>D5G5E1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Exocyst complex component Sec8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00004290001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SEC8 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835577.1">
        <property type="nucleotide sequence ID" value="XM_002835531.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5E1"/>
    <dbReference type="FunCoup" id="D5G5E1">
        <property type="interactions" value="273"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G5E1"/>
    <dbReference type="GeneID" id="9188153"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004290001"/>
    <dbReference type="eggNOG" id="KOG3691">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004025_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5E1"/>
    <dbReference type="OMA" id="HMEVRCR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006904">
        <property type="term" value="P:vesicle docking involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090522">
        <property type="term" value="P:vesicle tethering involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039682">
        <property type="entry name" value="Sec8/EXOC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007191">
        <property type="entry name" value="Sec8_exocyst_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048630">
        <property type="entry name" value="Sec8_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14146">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14146:SF0">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20652">
        <property type="entry name" value="Sec8_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04048">
        <property type="entry name" value="Sec8_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="3 4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component Sec8 N-terminal" evidence="5">
        <location>
            <begin position="4"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="Exocyst complex component Sec8 middle helical bundle" evidence="6">
        <location>
            <begin position="236"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF20652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ79734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="971" mass="108374" checksum="C62ADB446E019F04" modified="2010-06-15" version="1">MTLDSCVPVKVALQLMDTSSLGRAYQYDSYQQTHNQLQHALQSIVNDHHQGFNSSIGTFHSIMRSITTSQAKVRALRESLVQAKTDLSTSKPEVKNMVETSQTYDTMLRTLNLIEQLNGVPEKLEARISEKRFLTAVEVLSDALKVIKQPEMMEIGALSDLRTYLGSQESSLADILIEELHNHLYLKSPYCMDRWKPYSPHQKDSLLTKAEGKRPLHVFLDSVDFTQPVNANANKNPEADSLYYIRLLLEALNSLGHISNAVGTVNQRLPVELFKLVEKTNNEVDQRHPSSLTGVARSIGTFTKALDLGLSDNDVRVTVIHDLLWTLYSKFAAVMEGHRVIYDVVKGISRRDGSEDVASLAGGFMEVWQLIQSEMRSLLHDYLTMTDSRSVSSAPKGIHHAINNIVTGKTPRDKNKILFKLSSTDSNSASIKKDQDDLESILKASVPGLVSESLRPAATSSDVNTSSDGAATGHKLLIEPSVFNMGFLLPPSLAFLNRIKDIVPAGSGIVLSTLTSFLDDFLVNVFHPSLEDTIRDLFNQTTSDLDAFQQDPQWASVAKKPVMKGATAFLGLITALCKMLDTIPPDQAFGQLTIDLLNSYYEKCYEWYKELVARHRAGPEDGTTPMLDMKRSAQWARVDKIRSAMHGLWTGNEEAEALKKETEVELGDQEGKTLTLADLITDRKVTNSLCILYSSMKWLASNVVQLRHVEDTQSFSRQEGGLSGRRRRRWTLISGESSMSRDEAAKPVYLPMTTETVSGFDGVVNSFQELANTILFTLRAEARCHVLHYLDICMKEGNYCLDSLVNTPDPNVLALNADLVWFDEVISHLLRDTETKYLVKGLGAMMDHLLVANANCIKIINKEGVERMQLNILVLQQNLKNIETGGELTRASAFYALYAGGMEVIQTKFPKDLIKAAKEGKIDFSYDDLKVLVELYFSEAMSNRRESASALQARRSLNENLLALSEIMWTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5S0</accession>
    <name>D5G5S0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00001453001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430001">
        <property type="protein sequence ID" value="CAZ79863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835706.1">
        <property type="nucleotide sequence ID" value="XM_002835660.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5S0"/>
    <dbReference type="GeneID" id="9187541"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001453001"/>
    <dbReference type="eggNOG" id="ENOG502SU6X">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_634906_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5S0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42023">
        <property type="entry name" value="BHLH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42023:SF1">
        <property type="entry name" value="BHLH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="75"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="309"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="409"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="334"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="91"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="147"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="323"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ79863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="432" mass="47509" checksum="D531266BA714012D" modified="2010-06-15" version="1">MFNRLPIYTRPKEPAIGYPVLIESTNQTLTGLPSSQIVGFPQYLGGSSALRNSNESAFKGPSEKPVLSLNAGSHQSLYDDDLAPPPPLRINTLQYPSPPGSSNSNRTVGPGSNKQLNIPNHTMNAPPRKLNITRKPAPSSFSGANPTHSTHSTHSSDTFIASGLDKAATIDFSSSSSSHVQPRGRGFGLGNVVKGKVASRKRHDSDFGAESPIPIAAVKPPNFVSIDDWLDSREREGLHRRSNTVSTVATQVPRYNDPIKLHLRQSTFAPLAPPTEMSLNDSPAVPSIPAQYLPAPRSDSIRAVSAPLPPHEIHPIQRPASVPKDEPQEPKDHLSQLEFEQDQLTMRRAALRREIYDLEQVLPPNPSTHNPVARREMQARRDQLVQDLADVEKDLHEMGMKLHRAWRRRDKKMGTEGPTHLWVSRVTGSGDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5T8</accession>
    <name>D5G5T8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Translocase of outer membrane 40 kDa subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00001574001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Channel-forming protein essential for import of protein precursors into mitochondria.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tom40 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430002">
        <property type="protein sequence ID" value="CAZ79881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835724.1">
        <property type="nucleotide sequence ID" value="XM_002835678.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5T8"/>
    <dbReference type="FunCoup" id="D5G5T8">
        <property type="interactions" value="760"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G5T8"/>
    <dbReference type="GeneID" id="9187608"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001574001"/>
    <dbReference type="eggNOG" id="KOG3296">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042174_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5T8"/>
    <dbReference type="OMA" id="TRFNYRW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005742">
        <property type="term" value="C:mitochondrial outer membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015288">
        <property type="term" value="F:porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045040">
        <property type="term" value="P:protein insertion into mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07305">
        <property type="entry name" value="Porin3_Tom40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.10:FF:000009">
        <property type="entry name" value="Mitochondrial import receptor subunit TOM40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.10">
        <property type="entry name" value="Porin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023614">
        <property type="entry name" value="Porin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027246">
        <property type="entry name" value="Porin_Euk/Tom40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037930">
        <property type="entry name" value="Tom40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10802">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01459">
        <property type="entry name" value="Porin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="9" id="KW-0626">Porin</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00053390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00078731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ79881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="373" mass="39922" checksum="01B933FAD0A6A31C" modified="2010-06-15" version="1">MPTATAPILMGGKPVPSFLTENPITSLLSSLSHSFYERRATLGLGNPGTVENVSREVQKDVFLNNYAFTGMRADFSKSFSMDPIFQTSHAFAMGAQGIPPYTFAAIFGTNKAFLQANLDNEGQLAGRFNYRWLPELTSKFNIQLAPPSQPSQSVFTMDTDYSGSDFSAGIKAFNPSLLEGTLTGIFIGSYLQSITPNLALGVESVWQRSAASLGPETALSYVARYATKDWIASAQLQAGGALQATYWRRIAERVEAGVDCQLTLAGGMARDGGGGMMGGSMKKEGVTTLGAKYDFRMSTFRAQVDTQGKLACMLEKRVAPAVTLTFVGDVDHFKNQAKLGFSVSIESATEAIQAIQEKAALSGTGPTTPSVPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>D5G643</accession>
    <name>D5G643_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00001762001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430007">
        <property type="protein sequence ID" value="CAZ79986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835829.1">
        <property type="nucleotide sequence ID" value="XM_002835783.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G643"/>
    <dbReference type="GeneID" id="9187665"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001762001"/>
    <dbReference type="HOGENOM" id="CLU_1455406_0_0_1"/>
    <dbReference type="InParanoid" id="D5G643"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ79986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="186" mass="20712" checksum="771CDE24128E25FC" modified="2010-06-15" version="1">MQVSALMDEGRWLSFAEWVRLRDGLITEAWRDVLVGAIAGVSDCEGGEEDGIMCSRVITRICSHHPTSSHSRTRQQSFLFTLNPSRTYSKLRRAQPRPTLPHKPHLLFEGSLPIITMITGILPTKDLAMTREEFGRCFGVSDRGLVLSCRLRRCTRVLVGVVPHGGSPGDRVIGFAGYLLYLSTKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>D5G649</accession>
    <name>D5G649_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00001769001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
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                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="CAZ79992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002835789.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G649"/>
    <dbReference type="GeneID" id="9187671"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001769001"/>
    <dbReference type="HOGENOM" id="CLU_1185770_0_0_1"/>
    <dbReference type="InParanoid" id="D5G649"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="199"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="70"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="234" mass="26028" checksum="2986FAB174757769" modified="2010-06-15" version="1">MEGHSGRHDSGGLGEVCFYHNRIRIVTFFDNNAYPPPHSQHHHHQPRQANPTPPTSPTATASLSKKRAQSPSLTSSPSSPSSSPPPPPKRRVLLPSLPDETALLLTLKDTQNLPWPRVHTAFAAHGWERKLAVLKGRYKAIKEGGVYWEEDDVDLLFRAVWEVEREFERGRWEAVAEKVRGWGGCGGRVGSAECERRWKKGGRMEKEGEGEGEKVEEGQGKGREDVMKVGSLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5G698</accession>
    <name>D5G698_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00001670001</name>
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    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
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        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
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            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
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                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
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    <dbReference type="STRING" id="656061.D5G698"/>
    <dbReference type="GeneID" id="9187712"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001670001"/>
    <dbReference type="eggNOG" id="KOG0118">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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    <dbReference type="InParanoid" id="D5G698"/>
    <dbReference type="OMA" id="CAEQALG"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="P:mRNA splice site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12611">
        <property type="entry name" value="RRM1_NGR1_NAM8_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12613">
        <property type="entry name" value="RRM2_NGR1_NAM8_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12346">
        <property type="entry name" value="RRM3_NGR1_NAM8_like"/>
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    </dbReference>
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        <property type="entry name" value="mRNA binding post-transcriptional regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="mRNA binding post-transcriptional regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="mRNA binding post-transcriptional regulator"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
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    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
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    <dbReference type="InterPro" id="IPR050825">
        <property type="entry name" value="RBM42_RBP45_47-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47640:SF10">
        <property type="entry name" value="TRNA SELENOCYSTEINE 1-ASSOCIATED PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47640">
        <property type="entry name" value="TRNA SELENOCYSTEINE 1-ASSOCIATED PROTEIN 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="44"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="136"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="235"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="213"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="345"/>
            <end position="380"/>
        </location>
    </feature>
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        <location>
            <begin position="8"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="31"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="352"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAZ80041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5G707</accession>
    <name>D5G707_TUBMM</name>
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        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00004534001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
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        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430020">
        <property type="protein sequence ID" value="CAZ80300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836109.1">
        <property type="nucleotide sequence ID" value="XM_002836063.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="9182491"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004534001"/>
    <dbReference type="HOGENOM" id="CLU_1262334_0_0_1"/>
    <dbReference type="InParanoid" id="D5G707"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="172"/>
            <end position="219"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
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        <location>
            <begin position="46"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="108"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="219" mass="22156" checksum="FCFBCE137B4F92EF" modified="2010-06-15" version="1">MSTPPSISLTPAITTTATTTPPPPLPDDTDSDLATPPSTPPPPPLSSASTSSSSTATTASSPNPVFAPRPLRLPQFRHPRPLLLLDQFPLPLPRTPSPLTPPAPPACIPSSSSSSPKLARSFLASSPSPPAVGGGGELGRDVLEELFKIIRSVPGSPPAGAGPGAVGLQVHRRQGSAGGRGSPAAAAGGRECGFPSSPVSRTTMRNPIIFNSQFDGEST</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7B5</accession>
    <name>D5G7B5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00002428001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL5 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430023">
        <property type="protein sequence ID" value="CAZ80408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836217.1">
        <property type="nucleotide sequence ID" value="XM_002836171.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7B5"/>
    <dbReference type="FunCoup" id="D5G7B5">
        <property type="interactions" value="560"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G7B5"/>
    <dbReference type="GeneID" id="9185306"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002428001"/>
    <dbReference type="eggNOG" id="KOG0398">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061015_1_0_1"/>
    <dbReference type="InParanoid" id="D5G7B5"/>
    <dbReference type="OMA" id="HITIHTT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002132">
        <property type="entry name" value="Ribosomal_uL5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031309">
        <property type="entry name" value="Ribosomal_uL5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022803">
        <property type="entry name" value="Ribosomal_uL5_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031310">
        <property type="entry name" value="Ribosomal_uL5_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11994">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00281">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00673">
        <property type="entry name" value="Ribosomal_L5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55282">
        <property type="entry name" value="RL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL5 N-terminal" evidence="5">
        <location>
            <begin position="160"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL5 C-terminal" evidence="6">
        <location>
            <begin position="218"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="39"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ80408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="321" mass="35699" checksum="BFE1A641DD965976" modified="2010-06-15" version="1">MAHRTPPSFLWAPNTHHRLLLIPGNAFHQQSLKLSTTKPALARVPPGEKISASSPSKGGPRTRAKYQYRSPRYYRGRLAPNMPPPSASPSSRLFVPGPFAAERLEDHYWNTIAPDLQVLSYVHKQNPTFNIEKDYRQLTALRLPPKPTRQQLPALRTFRNIPRLMGITVHSMVRGALKDSAYLHAAGMVVQSITGVRATVHKSRENISMWELRAGKWISVTAQVKGRSAYRFLAQLIDVVLPRTKDWKGVAGGSGDSSGNITFGMSPEAVSMFPEIEVNYDMYPPRMVPGLNITIHTSAGNDVDAKILLKAMGLPFDGNFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7G5</accession>
    <name>D5G7G5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00002453001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430027">
        <property type="protein sequence ID" value="CAZ80458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836267.1">
        <property type="nucleotide sequence ID" value="XM_002836221.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7G5"/>
    <dbReference type="GeneID" id="9188483"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002453001"/>
    <dbReference type="HOGENOM" id="CLU_2851342_0_0_1"/>
    <dbReference type="InParanoid" id="D5G7G5"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ80458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="65" mass="7436" checksum="11B20EC508DB10EF" modified="2010-06-15" version="1">MSRIKIPGKMFRGGLVTTNKQWKYKMLGENGEWEKATMNAVQERRVCEKSGISNGCRDDMVLLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7G9</accession>
    <name>D5G7G9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00002459001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430027">
        <property type="protein sequence ID" value="CAZ80462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836271.1">
        <property type="nucleotide sequence ID" value="XM_002836225.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7G9"/>
    <dbReference type="STRING" id="656061.D5G7G9"/>
    <dbReference type="GeneID" id="9188487"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002459001"/>
    <dbReference type="eggNOG" id="ENOG502S59W">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_594425_0_0_1"/>
    <dbReference type="InParanoid" id="D5G7G9"/>
    <dbReference type="OMA" id="EEAWVDD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058602">
        <property type="entry name" value="YAG7_dimerisation_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26434">
        <property type="entry name" value="YAG7_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YAG7-like dimerisation" evidence="3">
        <location>
            <begin position="161"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="307"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="351"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="109"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="351"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="399"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="415"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="436"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="457"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF26434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ80462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="468" mass="51292" checksum="287BDAB472481CB4" modified="2010-06-15" version="1">MSSTTAVTQPNASRGKKKGKGGGGGTPSPAPELTQDKTADSTNDVKAGEKGEKEYLKWITKQLKKHTKKLDSIAKSEEKIKDLPEEEKATTKIINVDERNKIIGKPVTLAVYKELKECYENLLKASELEDKRLEAERVEAQARLEKAVEEAKAEAQKETDEAARAKILTVVKFLRLAGHRRNNKSVDEDEDEAIERVLVLVYGGDQSAIDACLNLANGSEEPVDGFQVSYSRIKEVAHALKIQGVDEEDVEETLEFAEEPETEQQQDQREELPTYEASAGNELDLAAQDEKVPLYTEGAAEDMQAPPQQAYTNGNEHEAPATAPGASIADGDATNYASKEVELEGIEDQVQAQITGETQAQDAPTTQPANGEPTGDSWADDQPKTPEVSDEFQNVERRGRGRGYRGQGRGRGRGYRGDRGGYRGDRSGYRGRGGRHRGEGGDPGDRPHRPRPKNTDRQPQPQQATTST</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7Q8</accession>
    <name>D5G7Q8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00004683001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430031">
        <property type="protein sequence ID" value="CAZ80551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836360.1">
        <property type="nucleotide sequence ID" value="XM_002836314.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7Q8"/>
    <dbReference type="STRING" id="656061.D5G7Q8"/>
    <dbReference type="GeneID" id="9187030"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004683001"/>
    <dbReference type="eggNOG" id="ENOG502S9RI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078580_0_0_1"/>
    <dbReference type="InParanoid" id="D5G7Q8"/>
    <dbReference type="OMA" id="MPKERNF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019007">
        <property type="entry name" value="Wbp11/ELF5/Saf1_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09429">
        <property type="entry name" value="Wbp11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Wbp11/ELF5/Saf1 N-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="210"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="222"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ80551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="270" mass="29699" checksum="BBCA99478EBF94DE" modified="2010-06-15" version="1">MQAWHKKEKAKQVKKGKAEKQKLRTEKLARRNPDRIQKQIDELQAIAEGGGHMSTGDRKLLADLQRDLAAVKKAKQTLGGGGEKVPAVPDRRAGVKRSAGEMEKKDEKRPPRPQHHHRQEEKGEEQGAEEEEESDSGESTSSSVRDIPMPAGTPPPLPTTTRAWPRGPRTAHQLPEKPASPAPAQTTYSAAPVLRDLRKEAVVFVPAAVKRKTFAGKATRSEEEEGGDGDGDGEEEEEGFVRKRINAAPDVDVEFSKFQAEMEEAPDEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5G850</accession>
    <name>D5G850_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00002818001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430039">
        <property type="protein sequence ID" value="CAZ80693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836502.1">
        <property type="nucleotide sequence ID" value="XM_002836456.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G850"/>
    <dbReference type="GeneID" id="9184166"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002818001"/>
    <dbReference type="HOGENOM" id="CLU_2514264_0_0_1"/>
    <dbReference type="InParanoid" id="D5G850"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="85" mass="9062" checksum="58D289E352A8E061" modified="2010-06-15" version="1">MTPMTRVEKPQEFCQTKSFLASAGWVLDDDIEHLGEVLAEAVGCCTLNSTTGGWDVSLAGGSEEAPGELLLPSLTTSDSWDSEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5G881</accession>
    <name>D5G881_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00002924001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CIA30 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430041">
        <property type="protein sequence ID" value="CAZ80724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836533.1">
        <property type="nucleotide sequence ID" value="XM_002836487.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G881"/>
    <dbReference type="STRING" id="656061.D5G881"/>
    <dbReference type="GeneID" id="9188326"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002924001"/>
    <dbReference type="eggNOG" id="KOG2435">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059028_1_2_1"/>
    <dbReference type="InParanoid" id="D5G881"/>
    <dbReference type="OMA" id="WIKAVAQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006120">
        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010257">
        <property type="term" value="P:NADH dehydrogenase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013857">
        <property type="entry name" value="NADH-UbQ_OxRdtase-assoc_prot30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039131">
        <property type="entry name" value="NDUFAF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13194">
        <property type="entry name" value="COMPLEX I INTERMEDIATE-ASSOCIATED PROTEIN 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13194:SF18">
        <property type="entry name" value="COMPLEX I INTERMEDIATE-ASSOCIATED PROTEIN 30, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08547">
        <property type="entry name" value="CIA30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:ubiquinone oxidoreductase intermediate-associated protein 30" evidence="5">
        <location>
            <begin position="44"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ80724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="249" mass="28728" checksum="4395B6E664D412F3" modified="2010-06-15" version="1">MRPALRLLRGGFLRRSIEEMKRNTFSVIKGEGITGDFADMEIHSFRHPDSLKNIKVFSDADTGGFSKVHMDLTPCPPGPLSEGQYYGRFRGNISIELPVERPKIQRSGYAAWRTLERGRTLFGRQYWDCDPYIYLALRVKSDGSKYFVNIQTDSIVETDIHQHRLFARRVGEWETIHIPFLEFVRTNFGQVIEPQNEMMKQKVKTVGIGLIDRIPGPFELCIDRIWATNTPEPEHVTKVPSSFNVHDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8A2</accession>
    <name>D5G8A2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00002947001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430041">
        <property type="protein sequence ID" value="CAZ80745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836554.1">
        <property type="nucleotide sequence ID" value="XM_002836508.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8A2"/>
    <dbReference type="GeneID" id="9188347"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002947001"/>
    <dbReference type="eggNOG" id="ENOG502S23D">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120561_1_0_1"/>
    <dbReference type="InParanoid" id="D5G8A2"/>
    <dbReference type="OMA" id="HACCAPI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003807">
        <property type="entry name" value="DUF202"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052053">
        <property type="entry name" value="IM_YidH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34187:SF3">
        <property type="entry name" value="DUF DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_6G11150)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34187">
        <property type="entry name" value="FGR18P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02656">
        <property type="entry name" value="DUF202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="55"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="84"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="DUF202" evidence="6">
        <location>
            <begin position="40"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ80745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="155" mass="17262" checksum="2A3DEBE47B272F6F" modified="2010-06-15" version="1">MSRYRPRPVFVDELQPNSPLILSSPFISPLVFENNASDARDHCANERNFLSWLRLSMYLCIVSVAVVISFHFKHEPSELEKELSLPLGICFWVLSLVCLFAGLGNYVKTIGMYSKKAALVQSGPKTQFVMVIVAVAVVAACVLFLSTHAKRTPDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8T0</accession>
    <name>D5G8T0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00004824001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430053">
        <property type="protein sequence ID" value="CAZ80923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836732.1">
        <property type="nucleotide sequence ID" value="XM_002836686.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8T0"/>
    <dbReference type="GeneID" id="9186283"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004824001"/>
    <dbReference type="eggNOG" id="ENOG502QTVA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017511_2_0_1"/>
    <dbReference type="InParanoid" id="D5G8T0"/>
    <dbReference type="OMA" id="CFIGWAG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037959">
        <property type="entry name" value="MFS_SpdSyn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01564">
        <property type="entry name" value="Spermine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ80923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="569" mass="62915" checksum="A1AD6DCB93AE33E5" modified="2010-06-15" version="1">MATTTPSKPSAKGMRAMARRAKISRNSPTIPNTLGFSKLLAVFKAVVLLSTASIMSCASQMALAPVYGGIPTSLYHSKISAFVFAAAWIAKYLPFKLPFKARNLLPVLSLYVPAIQRALARYSDGWGPFCGPIITEGVTFYPLLFLSVYSIAHLAHFGNFATDGVVASVFAGYFWNMQSSVPKHLMNRIGSSWILTRCGLPNLAGGIYALVSPSLLLLTAIPAMLHTNMYNSMCTSTIGLNEILTASNYTLLARQESNTGYISVLQHNHQNFRVLRCDHSLLGGVWEVPPPGFEYMDQGIKEPIYAIFVMLEAVRLIDPPPKNLNPKALAIGLGIGTAVNGLITHGVETDVVELDPVVHQYATEFFQLKENHTAYIEDAITFVKRENSNRGKDRKKYEYILHDVFTGGAVPASLFTLEFFVGLRDLLSEDGVIAINWAGDLKLKAAKSIIETVRTVFDGCRVFREDMPGEDIDKATVDYTNMVIFCTRSPDPYTFRAPADADILGSITRRHFLFPKFEIDLDQYFGVSGEFTLLGESNIDALIGWQETSALGHWRVIRTVLPPVVWENY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8V4</accession>
    <name>D5G8V4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00004855001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430053">
        <property type="protein sequence ID" value="CAZ80947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836756.1">
        <property type="nucleotide sequence ID" value="XM_002836710.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8V4"/>
    <dbReference type="FunCoup" id="D5G8V4">
        <property type="interactions" value="118"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G8V4"/>
    <dbReference type="GeneID" id="9182537"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004855001"/>
    <dbReference type="eggNOG" id="KOG3933">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1090674_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8V4"/>
    <dbReference type="OMA" id="WRMNEER"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019349">
        <property type="entry name" value="Ribosomal_mS35_mit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039848">
        <property type="entry name" value="Ribosomal_mS35_mt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13490">
        <property type="entry name" value="MITOCHONDRIAL 28S RIBOSOMAL PROTEIN S28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13490:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10213">
        <property type="entry name" value="MRP-S28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Small ribosomal subunit protein mS35 mitochondrial conserved" evidence="2">
        <location>
            <begin position="104"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ80947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="255" mass="29377" checksum="7663BED6AA31D6A2" modified="2010-06-15" version="1">MASSLSRTIVHLPRLARRTMPTTRPSARHLTSSPHPSYDQPILISQTPPPKPADRDPNEVEEKDWEGDDFTSSGHSELLRHREARSYARLAMYEMPLLSRLSKPFRPPSRAEVLRFRYTTYFGEAHPAESKVVVEFAPRDLVSLTAAQRGKLVKMVGARFDPERGVVKMSCEMFELQAQNKRYLSDLVDRLILEAKDESDDFGDIPFDFRHYTAKLKRPKARFPKAWRMNEERMASIREERAAFAKLFERGGVTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8W3</accession>
    <name>D5G8W3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00004866001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430053">
        <property type="protein sequence ID" value="CAZ80956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8W3"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004866001"/>
    <dbReference type="HOGENOM" id="CLU_2265672_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8W3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ80956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="103" mass="11029" checksum="2DC863BF5F9F3728" modified="2010-06-15" version="1">METLATRARSATSKYTSICSRISFGTPLMVSFVPWHSLSSGWMRSASASESRGVVCPAFMDALVGLLGFPFGGLGGCGCLWTDKAGWSEEGRGRFVKRKGNLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9A4</accession>
    <name>D5G9A4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">beta-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00003223001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.</text>
            <dbReference type="EC" id="3.2.1.21"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 3 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.21" evidence="3"/>
    <dbReference type="EMBL" id="FN430057">
        <property type="protein sequence ID" value="CAZ81097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836906.1">
        <property type="nucleotide sequence ID" value="XM_002836860.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9A4"/>
    <dbReference type="STRING" id="656061.D5G9A4"/>
    <dbReference type="GeneID" id="9187262"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003223001"/>
    <dbReference type="eggNOG" id="ENOG502QR4D">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004542_4_0_1"/>
    <dbReference type="InParanoid" id="D5G9A4"/>
    <dbReference type="OMA" id="REWINTP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009251">
        <property type="term" value="P:glucan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000495">
        <property type="entry name" value="Periplasmic beta-glucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1700">
        <property type="entry name" value="Glycoside hydrolase family 3 C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.300">
        <property type="entry name" value="Glycoside hydrolase, family 3, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050288">
        <property type="entry name" value="Cellulose_deg_GH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026891">
        <property type="entry name" value="Fn3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002772">
        <property type="entry name" value="Glyco_hydro_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036881">
        <property type="entry name" value="Glyco_hydro_3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036962">
        <property type="entry name" value="Glyco_hydro_3_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42715">
        <property type="entry name" value="BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42715:SF3">
        <property type="entry name" value="BETA-GLUCOSIDASE B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14310">
        <property type="entry name" value="Fn3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01915">
        <property type="entry name" value="Glyco_hydro_3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01217">
        <property type="entry name" value="Fn3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52279">
        <property type="entry name" value="Beta-D-glucan exohydrolase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibronectin type III-like" evidence="7">
        <location>
            <begin position="386"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ81097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="477" mass="51507" checksum="C6A9701A7419F46D" modified="2010-06-15" version="1">MPGPAHRRGENLLKAIEESNNDTKLLAAVKTGARHVLELIARSGKWGRFGEEPEMANDKPEHRALIRKTGAEGIVLLKNESNALPISTSVKKIAWIGPNANECIAGGGGSANLNPHYLTNPLDSIREAAKEHGIEVNYEIGCQTEKWVKTFPITGGREPPDTGNDSLDFASAVGIRLGVDLVRDAQEHISRAAALAGESDVAVVVVGMNNEWESEGYDRHGMDLPGKQNKLISAVAKANPRTIVVNQSGCAVSMPWLDEVQGLLQVWYQGQEAGNALADVLLGKRAPGGRLPISFPKRVEDNPSWGNFGDSGAESEVQYSEGVFVGYRHYVSREVPVLFPFGFGLSYTTFSLSSPRFAGGKNTLAPGATATVEMAIKNTGKVASSETVQVYISPRAGSPPVGRPTKELKGFAKVDLRPGEEKVVKVVLDEFAAGYFEPSVGKWRAEKGVYEVSVGRNVEDVVGKLEVIVEETYEWIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9C6</accession>
    <name>D5G9C6_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Aspartate aminotransferase</fullName>
            <ecNumber evidence="8">2.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00003243001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:21824"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="EC" id="2.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3 8">Homodimer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="8">In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.1" evidence="8"/>
    <dbReference type="EMBL" id="FN430059">
        <property type="protein sequence ID" value="CAZ81119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836928.1">
        <property type="nucleotide sequence ID" value="XM_002836882.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9C6"/>
    <dbReference type="STRING" id="656061.D5G9C6"/>
    <dbReference type="GeneID" id="9182870"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003243001"/>
    <dbReference type="eggNOG" id="KOG1411">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032440_1_2_1"/>
    <dbReference type="InParanoid" id="D5G9C6"/>
    <dbReference type="OMA" id="VGACTIV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004069">
        <property type="term" value="F:L-aspartate:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006533">
        <property type="term" value="P:L-aspartate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000026">
        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000001">
        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000796">
        <property type="entry name" value="Asp_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006719">
        <property type="entry name" value="PRK09257.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879:SF22">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00799">
        <property type="entry name" value="TRANSAMINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="9">
        <location>
            <begin position="41"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ81119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="410" mass="45552" checksum="6CD5DFFBAC150F33" modified="2010-06-15" version="1">MLSARIFFPCRSCKHLEQCYSRATCATGITEAYKADKFDRKVNLGVGAYRDDKGNPYVLPSVRAAEERILMKGLDKEYAAITGVLSFTKAAIELAYGKPSHALDRIAATQSISGTGALRIGGAFLERFYPFSKTVYLPTPSWANHAAIMKDSKINVKSYRYYNSQTIRLDIDGLLEDIGNAPKNSIFLFHACAHNPTGVDPTPEQWRAISEAVKSCGHFPFFDMAYQGFASGDTNKDAYALRYFIEQGHPVALSQSFAKNMGLYGERVGVFSLLAESAEEKRRLDSQIKILVRPLYSNPPVNGARIASEILNDLTLRKQWLSEVRGMADRIISMRAALKTNLEEIGSKHDWSHITSQIGMFAYTGLRPEQVDRLAKEFSIYGTKDGRISVAGITSDNVKYLAESIHKVTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9W6</accession>
    <name>D5G9W6_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">urease</fullName>
            <ecNumber evidence="2">3.5.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00005103001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Nitrogen metabolism; urea degradation; CO(2) and NH(3) from urea (urease route): step 1/1.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.5" evidence="2"/>
    <dbReference type="EMBL" id="FN430064">
        <property type="protein sequence ID" value="CAZ81309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837118.1">
        <property type="nucleotide sequence ID" value="XM_002837072.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9W6"/>
    <dbReference type="STRING" id="656061.D5G9W6"/>
    <dbReference type="GeneID" id="9188552"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005103001"/>
    <dbReference type="eggNOG" id="ENOG502QPKB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000980_3_0_1"/>
    <dbReference type="InParanoid" id="D5G9W6"/>
    <dbReference type="OMA" id="LISMEIM"/>
    <dbReference type="UniPathway" id="UPA00258">
        <property type="reaction ID" value="UER00370"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035550">
        <property type="term" value="C:urease complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009039">
        <property type="term" value="F:urease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043419">
        <property type="term" value="P:urea catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00407">
        <property type="entry name" value="Urease_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00390">
        <property type="entry name" value="Urease_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.150.10">
        <property type="entry name" value="Urease, beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.280.10">
        <property type="entry name" value="Urease, gamma-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050112">
        <property type="entry name" value="Urease_alpha_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002019">
        <property type="entry name" value="Urease_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036461">
        <property type="entry name" value="Urease_betasu_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002026">
        <property type="entry name" value="Urease_gamma/gamma-beta_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036463">
        <property type="entry name" value="Urease_gamma_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009671">
        <property type="entry name" value="PRK13192.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009682">
        <property type="entry name" value="PRK13203.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00192">
        <property type="entry name" value="urease_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43440">
        <property type="entry name" value="UREASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43440:SF1">
        <property type="entry name" value="UREASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00699">
        <property type="entry name" value="Urease_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00547">
        <property type="entry name" value="Urease_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51278">
        <property type="entry name" value="Urease, beta-subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54111">
        <property type="entry name" value="Urease, gamma-subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ81309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="231" mass="25722" checksum="3CA365FFE5D3B9DD" modified="2010-06-15" version="1">MQLIPRELDKLVISQAGSLAQKRLARGVKLNKSEATVFLFHFSLLIRDGKHSVAELMEEGKTMLGRRHVLPHVAISLKEIQVEGTFPDGTYLRPLVDASEYEHEKQPGAVVAVKGPKIVLNDERNRCILRVTNKGDRPIQVGSHYHFIEVNPQLEFDRIQAFGYRLDIPAGTAIRFEPGDSRTVNLVQIGGRKIITGGNNVANGRLEGKRIPKILENLQKGGFSHVPVFHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAB5</accession>
    <name>D5GAB5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00005222001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the SNAP family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430075">
        <property type="protein sequence ID" value="CAZ81469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837278.1">
        <property type="nucleotide sequence ID" value="XM_002837232.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAB5"/>
    <dbReference type="FunCoup" id="D5GAB5">
        <property type="interactions" value="858"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GAB5"/>
    <dbReference type="GeneID" id="9185751"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005222001"/>
    <dbReference type="eggNOG" id="KOG1586">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046329_1_0_1"/>
    <dbReference type="InParanoid" id="D5GAB5"/>
    <dbReference type="OMA" id="WSVKEYL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005483">
        <property type="term" value="F:soluble NSF attachment protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035494">
        <property type="term" value="P:SNARE complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048280">
        <property type="term" value="P:vesicle fusion with Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd15832">
        <property type="entry name" value="SNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000049">
        <property type="entry name" value="Alpha-soluble NSF attachment protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000744">
        <property type="entry name" value="NSF_attach"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13768:SF8">
        <property type="entry name" value="ALPHA-SOLUBLE NSF ATTACHMENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13768">
        <property type="entry name" value="SOLUBLE NSF ATTACHMENT PROTEIN SNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14938">
        <property type="entry name" value="SNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00448">
        <property type="entry name" value="NSFATTACHMNT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ81469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="291" mass="32348" checksum="289B1586B14799DB" modified="2010-06-15" version="1">MAQDPRALLAKADKQASSARGGFSLFGGKQDKLEIAADLYTQAANAFRIQKLGREAGQTLEKAAAIRQEINEPDDAASNLVEAYKAYKKDDPSSAARVLEKAIQHFTLRGNFRRAATYQQNLAELLDEIGDVPRAVAAYELAGDWFQSDNAESLANGAFLKMADLAALEGMYPKAVEKYEGVARSSAESNLMKWSMKDYFLKAGICHLASRDKIALTKALDNYVDLDPTFRSTREFQLLNDMLEADNSSDAEVFADKIFQYDQMSKLDKWKTTMLLRVKKSIEEEDDDGLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAI5</accession>
    <name>D5GAI5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00003604001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430078">
        <property type="protein sequence ID" value="CAZ81528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837337.1">
        <property type="nucleotide sequence ID" value="XM_002837291.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAI5"/>
    <dbReference type="SMR" id="D5GAI5"/>
    <dbReference type="STRING" id="656061.D5GAI5"/>
    <dbReference type="GeneID" id="9187468"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003604001"/>
    <dbReference type="eggNOG" id="KOG1902">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_284726_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAI5"/>
    <dbReference type="OMA" id="WESSGPF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990247">
        <property type="term" value="F:N6-methyladenosine-containing RNA reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21134">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.590.10">
        <property type="entry name" value="ph1033 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007275">
        <property type="entry name" value="YTH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045168">
        <property type="entry name" value="YTH_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357:SF3">
        <property type="entry name" value="YTH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357">
        <property type="entry name" value="YTH YT521-B HOMOLOGY DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04146">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50882">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YTH" evidence="2">
        <location>
            <begin position="941"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="586"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="715"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="760"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="278"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="348"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="380"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="391"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="445"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="590"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="647"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="779"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="825"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="893"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="904"/>
            <end position="920"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ81528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1090" mass="123509" checksum="8D04FE6B5AEEBEBC" modified="2010-06-15" version="1">MSLEGRISNPRSPEQRMDSHHPRQLRSYAHYNEGGRSHPDSRPMDGRANLRSYQQEVEISYDEKPRCPEDNTPTEGAYGHSEGDKSESGITDEGINQGHRSKSNGDISQGLNLDTPNNPGAASVGDATQNISKDEPEPNLTNDLTSETQQPGKGLNSNHHSSTEKESGNMANSKDTLSTKDPNELRQKVLESLAEKKKKAAVFITQESEGTGRKEASPESRVGPVGMNTTEIGSGNDKSPTRASASNPEREAAVNALLAEAMGSSKTVSGKVEKVQNAGASTTAKNSVKSPTEESFDQTRRRKNYPEEIKASGMTRGPSGEFHENTSPIYDDPETSRRSSFAGHHSYPRNDKQSRPKRSPEDLPRREGAYKEDYSHSSRQKISPTSASRQEFPRVQRAYEHEERDRNDARQDARQDARHRTDRFEDPPPSAKEFRHPPEKPGASRRGDGDYRGKYLEEEKLRTREEPSSYRSMDVAREDDPRDPYPRYSRRMVDPNWQDQGPDAPPYPTGRYLPPRDDHRLPPITRPPETDYAALYYKDLTEWLEVTGYHDHPYRQMVLRHQRDLRAMEDRRIVIEDERDIPRVYPGVPLREDVDPRDPRRTRGPPSYTLMPSEMPPRDERDPSSRVPESTAHRSSSRAALYPPLPRLDDRTRYQGDLRIASPQGEAVGLKRRAPFRDEYDDAGPVGKSARVNYENQHRSTTTLKSGDYVNAEQSGTHMKHHSGHTDPVDHRHPSRRITKARFEDPEDDAAGAVRQSLSKRIATGRGREQSPPPLPLEARVRERSLSPRHSYDNNDRKEGHKQGPPNKPHRDGDNGTAFVHPQRKGFEKGRRTPEPSGPRYEKDLQKPGRSVSGKFTPREDFRKPFPGRRSSSDDYRSPGNTLRRGSIDEHKKRPFGRGRGRGYTKEFHPSTRHNGKQDYGDPMDFENRVGPVIDTRGAGTRYFVVKSFNHDNVKMAQKDELWATQKKNSETFEEAFKTSRDVILVFSVNKSGKFQGYARMESAPGTAPVPTWAKNLLWESSGPFRIRWVTINDINFHRVAHLTNRLNEDQPVLIGRDGQEIDPECGAALCRLIDESANFHRIYNENGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAP0</accession>
    <name>D5GAP0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00003709001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430083">
        <property type="protein sequence ID" value="CAZ81583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837392.1">
        <property type="nucleotide sequence ID" value="XM_002837346.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAP0"/>
    <dbReference type="GeneID" id="9187750"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003709001"/>
    <dbReference type="HOGENOM" id="CLU_1696789_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAP0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ81583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="155" mass="17145" checksum="053CC0E5B54BF84A" modified="2010-06-15" version="1">MTVLTTSRIYSQKSIYTARGDFSVLSEGVDTYSWQVLRPEVHDGKCTLRFVGDQINPMVNTQLSWLKALAGASNVRECGDSQALKAAGDPAIFWGLIALSIERGVQPSGLTEVVNRGDWRIPPEEKHTGRVVVVDICCDPRTPKPKDYDYEFPGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAR4</accession>
    <name>D5GAR4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00003751001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="5">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the paraoxonase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430085">
        <property type="protein sequence ID" value="CAZ81607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837416.1">
        <property type="nucleotide sequence ID" value="XM_002837370.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAR4"/>
    <dbReference type="GeneID" id="9187774"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003751001"/>
    <dbReference type="eggNOG" id="ENOG502S58R">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_647570_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAR4"/>
    <dbReference type="OMA" id="YHAFRAF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004064">
        <property type="term" value="F:arylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002640">
        <property type="entry name" value="Arylesterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051288">
        <property type="entry name" value="Serum_paraoxonase/arylesterase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11799">
        <property type="entry name" value="PARAOXONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11799:SF30">
        <property type="entry name" value="SERUM PARAOXONASE_ARYLESTERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01731">
        <property type="entry name" value="Arylesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072039" evidence="6">
        <location>
            <begin position="26"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602640-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ81607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="424" mass="46488" checksum="E8AE8746299F0BE7" modified="2010-06-15" version="1">MAGSNKLKLAIVLLTVSAFLYKAITQRSPLYLLAPPFPLPPSISSSCSKNFPELLRNCEDFSILPNTTLLFLSCDRSRREWYHAFRAFPPDVPKGEIFIWDYDKPEVEPTLLSGAESLDDDFHPLGVSAVPREEGGEVRLFVTNQAKKEARIEVLDVDLETRTARHKRSLTDAKIFSPNGIVGVDENSFFITNDGFFSRRWSTALLFEVILGAPGGQVVHVSIPPLASATGKDSVDAYSVAWISLANGLALDHVKQKLYVASSTHGVYTYSIPDMSRPYLTVPESFHRTPFATDNLHLGPTSRLFASGITSLGDFATSMLSEGIVGPAAWTAELLPRVDPSDKREAALRALDPANSPLRKEEWVWRSVYLDDGKFFGGVSTGGIVDMQGTFVGVSVVGKGVLVCKRVIERPGVLVGEENVKEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAT4</accession>
    <name>D5GAT4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00005293001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430086">
        <property type="protein sequence ID" value="CAZ81627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837436.1">
        <property type="nucleotide sequence ID" value="XM_002837390.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAT4"/>
    <dbReference type="GeneID" id="9187794"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005293001"/>
    <dbReference type="HOGENOM" id="CLU_1732821_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAT4"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="151" mass="16829" checksum="2AA55E1A7AA32BD1" modified="2010-06-15" version="1">MSLPLSSQSVKLRHDHPKSKLPKKQAPHTRPTPPPSIRSLSSFLRSSRVPDFSIGAGRKRYYTRNSLKRAYFYPGYLLHERTNERKKEHKQSLKITKRRKCPSPTTKGSAGGGSDGVSLRIGRVLGHMLGLAAVGLIASDNLASLKLWWGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAT6</accession>
    <name>D5GAT6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00005300001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430086">
        <property type="protein sequence ID" value="CAZ81629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837438.1">
        <property type="nucleotide sequence ID" value="XM_002837392.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAT6"/>
    <dbReference type="GeneID" id="9187796"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005300001"/>
    <dbReference type="HOGENOM" id="CLU_370949_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAT6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="750" mass="84687" checksum="E8BE1EBE3412C9D4" modified="2010-06-15" version="1">MNSSSTSSESFRVQQDNTEDPPYDLSSNTPLEAFQVPLTILEDQEDNMNNSTLSPLEEMSDQSESPIQQTSLDLESTSKSPDTFYQFTKFPQEIQEKVIEEYIQLLSPNLSIIKDQAQIPITQDLNQQAESSQLLNHTIAYELENSLHNILLVSKAMYKMVAGYMTRNAVYIDGPFNLQRLLLFEKRQRKDWSNEIPTQKPIINRPTKIYPFGVTYSFTNENCGDVTGLAPGKIAESPYHFDSYGAGPWWKRPDSHDSNELMAQIYENDEEHGDWNWLNIPGWVRGGSYNIGGHLNLSDGSIDDNKSIKDYGSVEDSGDSDDRSQMEVDCGDGLNKGDYEPGSFLTHAKTLILGPGASEMHESDIPHYVSSLSRGQDTEMVAVYYRIPATHLLSKMFGRLDNLASLKKLVISGHIWSNPWLFVPRPVDSDLRFPNLTHLTLSLPFAAHKEASPLRSLRYFRERSADRHLCMLLTGRYRIPGGVVIENGSTFLNTLEYLHVRGPGAHCCSQLWKGCWKKLNSIIIELPTTAAICEPGTENLISAIKDNFLNRLPKAEVQVNLRTILPWFDSHFGHYFWRRMKDRIISTESYPGEALPLAHSPSGINVVLFSKSNEEHTIIQAHPDWLAAINNIQHEEKLHANGTIEYFKNRDGVDAMEMVWEQDHIWITAENHINTPAPAEENPGASEDTATVAPYQSGSPYLANVLKKEKGEPDYSTLIHAADDEFLRQEHEYLEHIANALKKGHTPGLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAV1</accession>
    <name>D5GAV1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00005320001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430086">
        <property type="protein sequence ID" value="CAZ81644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837453.1">
        <property type="nucleotide sequence ID" value="XM_002837407.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAV1"/>
    <dbReference type="STRING" id="656061.D5GAV1"/>
    <dbReference type="GeneID" id="9187812"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005320001"/>
    <dbReference type="eggNOG" id="KOG2406">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006241_2_0_1"/>
    <dbReference type="InParanoid" id="D5GAV1"/>
    <dbReference type="OMA" id="TKDYIWQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010770">
        <property type="entry name" value="Ecd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13060:SF0">
        <property type="entry name" value="PROTEIN ECDYSONELESS HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13060">
        <property type="entry name" value="SGT1 PROTEIN HSGT1 SUPPRESSOR OF GCR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07093">
        <property type="entry name" value="SGT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="382"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="426"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="574"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="584"/>
            <end position="594"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="594" mass="66805" checksum="70A917031B26E2F4" modified="2010-06-15" version="1">MEVNTDPFAPDFGQFHGTPQRTLPDDCMGYTLIIINQASDQSAIRDELEKVKQTADKLVEELTADYIWQRDSLQLELAETEDVGWYLHGRTEYGDSVDDEWLIVWILRELTRNIPNLWFRVYDTDGEFLLIEAASTLPRWLNPEVADNRVWIHDSKLLIIPLTVAPTLTDRSKSAAHPLTLKEAYSILTSDSCALLQRIRLVEEEAFYRLERYPKAIQEQLHYTEALIPRTCAMVLHEAPESIAAAVEAFYLRDPISLKSLKSMSHFPPSDMVTTSVRFTKVLYAQLKSQEFEPPSGSGFEVSPDMPTVNMIGIKLACGFEMLISDNAAPQVSSKARKNIVRRVKDILQTKTPPTDEEISSWPQRIDGEDWLDINYEDLERDLSGRNADPSGSSRGAKEAESGWGDKQAEETLKKMVKRFEAFLNDEDAGVEGAEVDDMDIDDDDDTEELSDSDEDKEVSFDEVEFSRMMREMMGMPMQASGDGEGVGEGEEAKIREVMAKVEEELKEAGAIERGKGMAKIQEDCGEGDVSDDGDEEVNVDYMLAKNMLESFKGQGGLSGPGGNLLARMGIALPRDEPEEDEKYYETKGKQIQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBB2</accession>
    <name>D5GBB2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00000400001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430090">
        <property type="protein sequence ID" value="CAZ81805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBB2"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000400001"/>
    <dbReference type="HOGENOM" id="CLU_2869278_0_0_1"/>
    <dbReference type="InParanoid" id="D5GBB2"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ81805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="64" mass="7078" checksum="60A23DB133A379D0" modified="2010-06-15" version="1">MYSCIIAFPPPFIRPRNSNYAATSTALVPVRAHAQFHHASRFPNYSFTRASIIAANASKLQGNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBC1</accession>
    <name>D5GBC1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00000410001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM208 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430090">
        <property type="protein sequence ID" value="CAZ81814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837623.1">
        <property type="nucleotide sequence ID" value="XM_002837577.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBC1"/>
    <dbReference type="STRING" id="656061.D5GBC1"/>
    <dbReference type="GeneID" id="9184388"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000410001"/>
    <dbReference type="eggNOG" id="KOG3269">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094308_1_0_1"/>
    <dbReference type="InParanoid" id="D5GBC1"/>
    <dbReference type="OMA" id="GRPKYDA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006624">
        <property type="term" value="P:vacuolar protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008506">
        <property type="entry name" value="SND2/TMEM208"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13505">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13505:SF7">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05620">
        <property type="entry name" value="TMEM208_SND2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="92"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="115"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="144"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="144"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ81814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="176" mass="19100" checksum="ECB428B527F688E8" modified="2010-06-15" version="1">MANKAAKQLARANTATLNRAHITTLALHTLFILYRLLYRSGSWSKYAALSLPSLVVEIYLERLGRPKYSSPGGDGVLVSAGEDMEAKGVTEYMWDIVYVTWGVLGLVGVVGEWGWWVLAIVPIYAVYLAVGMYRGMSGLMPGMSSSPSAAAAAPDGPRSNRQKKAEKKGGQKARYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBF0</accession>
    <name>D5GBF0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Succinate-semialdehyde dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="2">1.2.1.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">NAD(+)-dependent succinic semialdehyde dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00000440001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.24" evidence="2"/>
    <dbReference type="EMBL" id="FN430093">
        <property type="protein sequence ID" value="CAZ81843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837652.1">
        <property type="nucleotide sequence ID" value="XM_002837606.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBF0"/>
    <dbReference type="FunCoup" id="D5GBF0">
        <property type="interactions" value="394"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GBF0"/>
    <dbReference type="GeneID" id="9184416"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000440001"/>
    <dbReference type="eggNOG" id="KOG2451">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_5_3_1"/>
    <dbReference type="InParanoid" id="D5GBF0"/>
    <dbReference type="OMA" id="VAACFRF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004030">
        <property type="term" value="F:aldehyde dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004777">
        <property type="term" value="F:succinate-semialdehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046394">
        <property type="term" value="P:carboxylic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009450">
        <property type="term" value="P:gamma-aminobutyric acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07103">
        <property type="entry name" value="ALDH_F5_SSADH_GabD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000026">
        <property type="entry name" value="Aldehyde dehydrogenase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000005">
        <property type="entry name" value="Succinate-semialdehyde dehydrogenase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050740">
        <property type="entry name" value="Aldehyde_DH_Superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43353">
        <property type="entry name" value="SUCCINATE-SEMIALDEHYDE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43353:SF5">
        <property type="entry name" value="SUCCINATE-SEMIALDEHYDE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="8">
        <location>
            <begin position="29"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="8">
        <location>
            <begin position="299"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00030806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ81843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="449" mass="48720" checksum="54CDCDE8E0632F5F" modified="2010-06-15" version="1">MPLSSHIVPKLKDPSLFHEAAFINGQFVKAKDGKTFDDSINIARDALKSFRKTLPRERARMLRKWYELIMENQEDLATLITRENGKPLADARSEVAYAAAFFEWFSEEAPRIYGDSIPASVRGNRVVTVKQPVGVCGLITPWNFPAAMITRKIGPALAAGCTVVAKAPGETPLTSLAIAELASRAGIPKGVVNIVTALKNTPEIGRELCTNPIIKKVSFTGSTSVGKLLMQQCSSTLKKLSLELGGNAPFIVFDDADLDKAVAGAIISKFRSSGQTCVCTNRIYVQDAVYSEFQRESFKFQTHVEDALKKGATVLTGGKGLGHLGPNFYEPTVITNMTSDMRIHREETFGPVAALFRFKSEKEVVNLANEAEVGLAGYFFSSDIARIWRVAEALEVGMVGVNTGLISDAASPFGGVKESGFGREGSKYGIDEYTIIKSITFGGVDDLQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBM1</accession>
    <name>D5GBM1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 16">Branchpoint-bridging protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">GSTUM_00005703001</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13 16">Necessary for the splicing of pre-mRNA. Has a role in the recognition of the branch site (5'-UACUAAC-3'), the pyrimidine tract and the 3'-splice site at the 3'-end of introns.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the BBP/SF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ82027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837836.1">
        <property type="nucleotide sequence ID" value="XM_002837790.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBM1"/>
    <dbReference type="STRING" id="656061.D5GBM1"/>
    <dbReference type="GeneID" id="9185462"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005703001"/>
    <dbReference type="eggNOG" id="KOG0119">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016864_3_0_1"/>
    <dbReference type="InParanoid" id="D5GBM1"/>
    <dbReference type="OMA" id="PGMPSMY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000243">
        <property type="term" value="C:commitment complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045131">
        <property type="term" value="F:pre-mRNA branch point binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048024">
        <property type="term" value="P:regulation of mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02395">
        <property type="entry name" value="KH-I_BBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.60.10:FF:000030">
        <property type="entry name" value="Branchpoint-bridging protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000024">
        <property type="entry name" value="Branchpoint-bridging protein-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045071">
        <property type="entry name" value="BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055256">
        <property type="entry name" value="KH_1_KHDC4/BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032570">
        <property type="entry name" value="SF1-HH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047086">
        <property type="entry name" value="SF1-HH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208">
        <property type="entry name" value="RNA-BINDING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208:SF45">
        <property type="entry name" value="SPLICING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22675">
        <property type="entry name" value="KH-I_KHDC4-BBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16275">
        <property type="entry name" value="SF1-HH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 16" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="11 16" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="12 16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="10 15" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 16" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="9 16" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 14 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="19">
        <location>
            <begin position="316"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="19">
        <location>
            <begin position="341"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="352"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="405"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="262"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="18">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="352"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="18">
        <location>
            <begin position="371"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="18">
        <location>
            <begin position="423"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="489"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="511"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="543"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="569"/>
            <end position="623"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00053279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU367126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CAZ82027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="623" mass="65633" checksum="9161001FD41723F5" modified="2010-06-15" version="1">MSYRGSGGGITGTNNIPLGNRRRFGGDGSTGSNDSPSPASGGPSFRDEQGGSGFADHKRGRSPIRSDPDGPKKRKKKNRWGDQTDNKAAGLMGLPTAIFATMTSEQLEAYALHLRIEEISQKLRINDVVPADGDRSPSPPPQYDNFGRRVNTREYRYRKRLEDERHKLIDKAMKAIPNYHPPSDYRRPTKTQEKIYVPVNDYPEINFIGLLIGPRGNTLKKMETESGAKIAIRGKGSVKEGKGRSDAAHTSNQEEDLHCLIMADTEEKVNKAKKLINTIIETAASIPEGQNELKRNQLRELAALNGTLRDDENQACQNCGQIGHRKYDCPEQRNFTANIICRVCGNAGHMARDCPDRQRGQDWRNNDRGGRGGAAGGFGRPGAAGLGQGDAVDREYEQLMQELSGGSAPAIGGATPQGRIEAGPGGPDGGYGGRESYGGGAYGRDNQNDQELGPDGRALKPWQRGPTGGPAPWQMGGRGRGGAPGDSHTPPTNAPTGPSGAPWQSQSHGPPTGPASSAPPPWAGTHGIPPPPPPASAAPPPWASGAPGAAPGMSYGASPYGAPPGGYGAPPPPPPSFGIPPPPPPPGNPPPPPPSSLAPPPPPPPPPPSSNEPPPPPPPSHYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBP4</accession>
    <name>D5GBP4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00005509001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the EFG1/PHD1/stuA family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ81894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837703.1">
        <property type="nucleotide sequence ID" value="XM_002837657.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBP4"/>
    <dbReference type="STRING" id="656061.D5GBP4"/>
    <dbReference type="GeneID" id="9182931"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005509001"/>
    <dbReference type="eggNOG" id="ENOG502QW2C">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016460_0_0_1"/>
    <dbReference type="InParanoid" id="D5GBP4"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048315">
        <property type="term" value="P:conidium formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030435">
        <property type="term" value="P:sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.260.10:FF:000003">
        <property type="entry name" value="Ascospore maturation 1 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.260.10">
        <property type="entry name" value="Transcription regulator HTH, APSES-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029790">
        <property type="entry name" value="EFG1/Phd1/StuA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036887">
        <property type="entry name" value="HTH_APSES_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018004">
        <property type="entry name" value="KilA/APSES_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003163">
        <property type="entry name" value="Tscrpt_reg_HTH_APSES-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47792">
        <property type="entry name" value="PROTEIN SOK2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47792:SF1">
        <property type="entry name" value="PROTEIN SOK2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04383">
        <property type="entry name" value="KilA-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01252">
        <property type="entry name" value="KilA-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54616">
        <property type="entry name" value="DNA-binding domain of Mlu1-box binding protein MBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51299">
        <property type="entry name" value="HTH_APSES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0183">Conidiation</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0749">Sporulation</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH APSES-type" evidence="8">
        <location>
            <begin position="248"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="366"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="489"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="12"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="51"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="165"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="187"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="373"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="416"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="446"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="492"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="528"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="556"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="578"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="598"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="613"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="662"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="689"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="745"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="764"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="826"/>
            <end position="841"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ81894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="862" mass="91872" checksum="722FC176BA5A3EC6" modified="2010-06-15" version="1">MSSDKPPAALSIPQSANNVPNNSSPRSLGTSILNSHSSTTPNGSLKVKTPTGPNAPTPQSIQHSLPNLNIPSPQHNTQPYHPSNPGPGSLHNSQFAYGSDPGFMNSQTNTPYVGLDFSHHHNLSQGQPPTPQTATSGSNMSFPPQPPVLQPSYSQPHHGFPPPFFSSQGGVSSPAGQPGVSGPMSVHATQQLLPLPQNSQMTPSPSPLASVSQGGPQTPSSATSATGFGNQPPFDTTGQHAPPGMKPRVTATLWEDEGSLCFQVEAKGVCVARREDNHMINGTKLLNVAGMTRGRRDGILKSEKVRHVVKIGPMHLKGVWIPFDRALDFANKEKITELLYPLFVHNIGALLYHPANTNRTNAVMAAAHRRQQDNAALQQQQQNQMQRRNPSELVPSSQPPMHHHHAMQHPMHTSLPPHPNLAPHPPQNQLQRPGLERSISFPTPPSSASSVMGVGNSESPTFWNGNMVSSVGGTGGQPSTIETVMNSARSMPTTPATTPPGGALQQLQQYPPPPSLYPSNPSAMAQHSIAQQNMQRFGQPLTQPPQYMNQRQEPSPMGPPSSRGPPAPLSRPSSRQNTADDTHPKEENVEEQNGLAVEGEEHEDHGEEEAEHEPEHEEEYTHDPNYANGHRTGNYYPSMQGDHTPHLSPEMTGSPNHGGQGPSTPARSTYTGSSVSVPRTVDGGPGATPRTTNASQQWVQSGYSTPPRTNSTNGNAIRQPPARGLYQLVNGGQDAAADHSGPNGTGAQDSGYQNQPGMGVSIPAQGTPQTFGVNGASTPGSNKRIREMDDEDEHGSRPSSRGQDERSGGDSEGGIGGLKRRKTIREGSTPTVGGINSSTFDRNTDGRLNRARSAIGTTRGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBV2</accession>
    <name>D5GBV2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00005598001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ81952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837761.1">
        <property type="nucleotide sequence ID" value="XM_002837715.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBV2"/>
    <dbReference type="STRING" id="656061.D5GBV2"/>
    <dbReference type="GeneID" id="9182989"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005598001"/>
    <dbReference type="eggNOG" id="ENOG502QT5J">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004835_0_1_1"/>
    <dbReference type="InParanoid" id="D5GBV2"/>
    <dbReference type="OMA" id="RDKGTEC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053181">
        <property type="entry name" value="EcdB-like_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47785:SF4">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47785">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="3">
        <location>
            <begin position="319"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="412"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="542"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="644"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="708"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="965"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="37"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="64"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="111"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="160"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="178"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="200"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="217"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="236"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="253"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="263"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="429"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="644"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="664"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="709"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="738"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="965"/>
            <end position="985"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ81952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1130" mass="121700" checksum="F88D190E4E3454E0" modified="2010-06-15" version="1">MSATNSDSKRPRLESWGSSSSPLSHEIHHTHSQHFPSQSSSAYSTTSPTASFADWQMKTPPQPVQHTLSHSRSHSIPTLPSGTVAGGKNGTLQQQQHPGHPLANKSAVAVSPQTSPPVHSSGPSPNHGYNNGFQTQHHRQLQGQQVSMDGPAPTPPPTGSTLHLRNATDQQQVQQLRSFSLSGNSSSSGHPSHYQPDTSGYYGQQMQGQRQQDLSHPGNSSGASANGDNRPPALFDSTSPGSSATLPPGPSPIMTSGFSTTPTQMMSHQHMSTSTSGVTSPVVTTPTVGGIPGTMMGNPQMNVVTTYPPRRKAIRAAQACDACRARKAKCDEGRPSCGFCKESQIACVYREVPPPKQDRTLLQILERLGRVEALLDSINSGREVTTVAVPTGGNRVVDGATAPKEATAVNETSLQSLQSTSPFHPPTPTTQQAYPQSTSGGASVQDHPRPGNPTEVPLEEDEQLTIPVQHTTAAHKLMWWPSIRKNIDEEFLANEGYVMKKEERRGPLRVYGRGEGFDSPGSMFEDMDDHDTGFVWDSGIGADEGVEGSRRGSEDSPMEDVLPEAGDYTNSQRKPSGYTGSGLTTGGGLKLDRQTVLTLLNSYLANIHILHPVVDKATITSMVFLFSERVASGDGSMLSLGLHRRNSSSAKRKRSISGIGPTHSMPSSQGMGPSPQRIPRTLHSALVLLVMALGAICSHKRPIPGPLSTLPNSPGFRTTTPPFKTPGSSYHRAGSPFHSYSQQQQHSGRLQGRQGRELKNIDVIPGLAYFVKAIDIMGVLSGGNELENVQICLLAGLYWGQLGRVLDSWKWINYACMGCQILVRMRLEGESNELRRDLILRAFWSCLQLESDVLAELDLPPSGISRLEDSMPLPSSVTDNKVKLDSPRSLAIARELDFQLEQWKLHLPEALRWEESDPPPADINAARLRAKYFGARYIIHRPFVYHAIHGGNGVYNAPNSGGVFSTSSPARSASEMSQSSPSATTPGGFNPLGNTAGSGVRRISIAGESERAELAPVSLEASCRKCIDSAISSTTAFHAFSPDVNRPVITNVFGTAHAQFGNLLVLQAAYQSPNLKSMIPQPLLAELFQKTIHWLQTLSPISSALKHDAQILENAAAKLDFQISTSMGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBZ2</accession>
    <name>D5GBZ2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00005718001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430098">
        <property type="protein sequence ID" value="CAZ82035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837844.1">
        <property type="nucleotide sequence ID" value="XM_002837798.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBZ2"/>
    <dbReference type="STRING" id="656061.D5GBZ2"/>
    <dbReference type="GeneID" id="9187256"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005718001"/>
    <dbReference type="eggNOG" id="KOG4302">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013892_0_0_1"/>
    <dbReference type="InParanoid" id="D5GBZ2"/>
    <dbReference type="OMA" id="WLFACDE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990023">
        <property type="term" value="C:mitotic spindle midzone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051256">
        <property type="term" value="P:mitotic spindle midzone assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007145">
        <property type="entry name" value="MAP65_Ase1_PRC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19321:SF41">
        <property type="entry name" value="FASCETTO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19321">
        <property type="entry name" value="PROTEIN REGULATOR OF CYTOKINESIS 1 PRC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03999">
        <property type="entry name" value="MAP65_ASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="461"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="227"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="486"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="503"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="528"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="565"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="592"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="653"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="687"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="731"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="746"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="775"/>
            <end position="785"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ82035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="825" mass="91343" checksum="1EBC84906CF4024C" modified="2010-06-15" version="1">MTTKHQSSLMEEVSKAQETLHCLFDEIGLTRYDREGRQASLFAALNTALQDQMRSVIAEKNTLVEECNKVISSIKQMEKSLDDKTKKSREELKITAPLLRCLEALRGKQENVKARHAERYNAIRELARSVEEYADRLESGITQVPLPPATNDSSSIASFDLSHAYYERLENERSRLRKEYTKRLANVTSLAKDIINLYAELGATGSGVDKTIIEFGATNPEKLGLKLDNLNDLIGKKERLVKEKEKRKEKIEDLKQEIGQLWEKLGEEPSEGKRFLAQNRGIGLKVIRELEAELDRLLELKRQNLQLFVEDARQVLQDLWDKLYFSDDEIMHFQPAIADVYTDALLTSHEMEIRRLQLLLEERAPIISLIDKHRSLVNDREQLAISASDSSRLVSRGGSGNRDPTRLLREEKMRKRIAKELPKVEVELKKILEDWEKECGEPFLVNGDTYLQTLIRESPVLQSRAGSKGPAPAQATVKAGKWNPPATGSIQRVRSNTAGSGQPRPPSRPKTPSGPPMRSKTPTGGSTLVSASVGRSGGRSVAASLSRSNSVRTPATSARLPIPHTTASSTSTTSSNASSGSRTPTRLRAPASLSSFPDATSSDKSTTRLVRSQSVKQLERTGLSNTLGTAPRMGQLLPSQTMGPPQLPPYLRQQREKDHERERERGRELQRPSSTSRTMSREGTVMSVRSVSPESQFGGSDDNEDQRDYCEGTPRQNGYPKPPVKSLGYHESSSSNGDAESTRMFSGSSTGTTATSASSNENWQTFGDESEEEGSIGGGNGGGYYRRGYAGYPSKRVGGGGGVVMVRNEEDEAEEWEEGEAAGRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5GC74</accession>
    <name>D5GC74_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Mediator of RNA polymerase II transcription subunit 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00000613001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 10">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the Mediator complex subunit 7 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430103">
        <property type="protein sequence ID" value="CAZ82117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837926.1">
        <property type="nucleotide sequence ID" value="XM_002837880.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GC74"/>
    <dbReference type="FunCoup" id="D5GC74">
        <property type="interactions" value="702"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GC74"/>
    <dbReference type="GeneID" id="9184793"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000613001"/>
    <dbReference type="eggNOG" id="KOG0570">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065214_0_0_1"/>
    <dbReference type="InParanoid" id="D5GC74"/>
    <dbReference type="OMA" id="MMQDHLD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037212">
        <property type="entry name" value="Med7/Med21-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009244">
        <property type="entry name" value="Mediatior_Med7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044888">
        <property type="entry name" value="Mediatior_Med7_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21428">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21428:SF11">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05983">
        <property type="entry name" value="Med7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140718">
        <property type="entry name" value="Mediator hinge subcomplex-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0010">Activator</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="40"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ82117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="258" mass="28225" checksum="E85800CC30ED6E08" modified="2010-06-15" version="1">MPQEPPPGALSSAFPPPPTYYTHFTEENLTALKSHRLEDGTLPPDDTLPDPLCYLVPPPPPAGSYRAFGEEWMVPERYPSLEEAGIRQLYPPTSMAAVAAANGGGGGGGADKGKELTVDRTVELRRLSKSLLLNYLELVGVMGMAPEQFHEKTADLETILFNMHHLINEYRPHQARETLCLRMEEQLERTRRETEENRKAVAKVEDILAGLELVAKRADAICGGDGLGAGREKKVDDGVAKARVRDVEAWKALVGMGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5GC79</accession>
    <name>D5GC79_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="17">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">GSTUM_00000631001</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RBT5 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430105">
        <property type="protein sequence ID" value="CAZ82122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837931.1">
        <property type="nucleotide sequence ID" value="XM_002837885.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GC79"/>
    <dbReference type="STRING" id="656061.D5GC79"/>
    <dbReference type="GeneID" id="9184800"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000631001"/>
    <dbReference type="HOGENOM" id="CLU_484130_0_0_1"/>
    <dbReference type="InParanoid" id="D5GC79"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21699">
        <property type="entry name" value="JMTM_APP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008427">
        <property type="entry name" value="Extracellular_membr_CFEM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051694">
        <property type="entry name" value="Immunoregulatory_rcpt-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15549:SF27">
        <property type="entry name" value="CHITIN-BINDING TYPE-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15549">
        <property type="entry name" value="PAIRED IMMUNOGLOBULIN-LIKE TYPE 2 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05730">
        <property type="entry name" value="CFEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00747">
        <property type="entry name" value="CFEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 15" id="KW-0732">Signal</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072281" evidence="15">
        <location>
            <begin position="35"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="170"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="CFEM" evidence="16">
        <location>
            <begin position="48"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="126"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="274"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="306"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="352"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="126"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="316"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="352"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="369"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="416"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="452"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="494"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="510"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAZ82122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="563" mass="59850" checksum="48ECE000033F81FA" modified="2010-06-15" version="1">MSHKRLSSSPSSSSWPGLFTLLLTVLLRVPTTSAHSPLQARQGNQLQGAEVLPPCALQCVYNAISQHPHFRMCHGQLECLCGENPQPYRELLWSCIDDQRHCRPEAHKETEAAVVSVCSGVLDASSSSSSNTTSPTSAAPASPSKTGTSQSTTPPSAKEESKKTSKLSTAAIVGISVGAVALLGLIVVTCFLLTIMRNRRKGREQIRSIAFEQASPRSPGEGWEAHGMDHSRWASVNPADPNSSAGHYYNSKSQLTPIVTDIPTEKRGVPAINISPVSPVSPSGNRAAAPPTKTKVVASRKIIENHHLQSDMPPSFSFTPATPATHWSTEEPVSPVSPMFDFNGARLARSSSNSVSSVSPSQGFLPGQAFTTDSRSVRSTSPSVEGSNRHTCSAATMPRIPRENSPEPSTPPPQYAPATPSPSGNTDSGRSSRNLSGAAAAGGGMARTDSISWRRELEVAAERAMARVVRSEQSIQEEGPGERRRKSSIPGLDRFARRASRDTRGDDDSSLASNGETGSSFWNRKGGRVSTPPRSPGFFNPGRRRDSGATGSKRSSADVGNVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCB6</accession>
    <name>D5GCB6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00005806001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430109">
        <property type="protein sequence ID" value="CAZ82159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837968.1">
        <property type="nucleotide sequence ID" value="XM_002837922.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCB6"/>
    <dbReference type="STRING" id="656061.D5GCB6"/>
    <dbReference type="GeneID" id="9184836"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005806001"/>
    <dbReference type="eggNOG" id="ENOG502S3KS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_330685_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCB6"/>
    <dbReference type="OMA" id="KNPHTHD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36721:SF1">
        <property type="entry name" value="OS04G0446401 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36721">
        <property type="entry name" value="PROLINE-RICH FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="634"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="680"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="114"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="216"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="259"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="649"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="703"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="754"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="790"/>
            <end position="838"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="867" mass="95621" checksum="1752771DB9622574" modified="2010-06-15" version="1">MLQNSLHTSSPLSPLSSPISPPPTAEPTAPHQDFSSSSSNPPHLSTRQRLPSSYKPPYTRPRSAEPVGFRSPVLVSQGFGTLPTLPATNSIPTTLSSADIGEEEEEDLGHEGVSLSAADQSPSAPSRSPPPPPRIDITPAHSERADMPFKLPQQQKREGPSPRHKPTPAPVPRYQALETPVPSTLSRAMNKDGIVRPASANANPIPAFSLPPSPPLTRRSTVVQSVRSSAPKYAPSLPPLQPSRSLAPGQDGTLLGRPSTSSSSSTVSVREVTKLGKALGRQPIDYLIPYGALDVPVSDNFHPYQDPHSLTNSPKTELPVADVFGNLNYLLESYLGILSNGGSMAVGTGYRSVARKLLQQVGGVFNREVGVEGIDWSDILEYIRGDEQKPCLCVLPVKGLIQRNIDTATILSGAEPASEVGLQAPQQTKELAESVVAGVREKSGTGKVQEWLEEVEKVLRQQPPPSPTVSTVPEEVEHEYEDEVVTQIIEGILPTPAQIEVFRSYLTDHAYLASTRAALMRLYPQHPAVSITKPIVALYLLLHVELHPMLAAIGGLSDAEMELINSGRFDGFLDGSTNAVPRDEEEELGCITDLHKKLARVMYTLDDELAELHNRALIVRQALKERIDAIATRMKKKAPESEYGGDNTVVDGGEDVAVGGEDEQDDLLSPLVPVEEWDGGKSILLSPDDSASNVHFSRRRREARQEMEESEKKRAEKKERKEIKRREEERRAGRHEPQEDCAASTTVSKRRKEKKGERRDRRSGEGTESGSSTKRGRSRRRGEGEEQDGEREKKEHKERKDEHRERKEERESRREERESRREEKERELREKDKKDKSWSRALGGINIKWGEKAGDMGAVAPEKERKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCC2</accession>
    <name>D5GCC2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NTF2-related export protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00005812001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Facilitates protein transport into the nucleus. Could be part of a multicomponent system of cytosolic factors that assemble at the pore complex during nuclear import.</text>
    </comment>
    <comment type="function">
        <text evidence="3">Has a role in nuclear-cytoplasmic transport of proteins and mRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430109">
        <property type="protein sequence ID" value="CAZ82165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837974.1">
        <property type="nucleotide sequence ID" value="XM_002837928.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCC2"/>
    <dbReference type="FunCoup" id="D5GCC2">
        <property type="interactions" value="1146"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GCC2"/>
    <dbReference type="GeneID" id="9184842"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005812001"/>
    <dbReference type="eggNOG" id="KOG2104">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1788222_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCC2"/>
    <dbReference type="OMA" id="HIKFSQM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00780">
        <property type="entry name" value="NTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.50:FF:000005">
        <property type="entry name" value="Nuclear transport factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045875">
        <property type="entry name" value="NTF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002075">
        <property type="entry name" value="NTF2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018222">
        <property type="entry name" value="Nuclear_transport_factor_2_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12612">
        <property type="entry name" value="NUCLEAR TRANSPORT FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02136">
        <property type="entry name" value="NTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50177">
        <property type="entry name" value="NTF2_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NTF2" evidence="4">
        <location>
            <begin position="18"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00053082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU369002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ82165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="145" mass="16337" checksum="64B7EA141FC37CE1" modified="2010-06-15" version="1">MTPHLVSFPPPPPTSSEKQRYELTKFSHHGRFPYCCEGVCCLGDRSGLGIVYRDHSMMTFETNQVQGLENIKEKLGSLPFRKLVHQITTLDAHPSAQSGSIIVLITGQLLIDDGEHPQKYSQCFHLIPDAGTYYVLNDIFRLIYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCU9</accession>
    <name>D5GCU9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00000757001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430123">
        <property type="protein sequence ID" value="CAZ82342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838151.1">
        <property type="nucleotide sequence ID" value="XM_002838105.1"/>
    </dbReference>
    <dbReference type="GeneID" id="9183805"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000757001"/>
    <dbReference type="HOGENOM" id="CLU_678244_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCU9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="406" mass="45708" checksum="AF6DE240983058FD" modified="2010-06-15" version="1">MIFLPTVFRSFFPLFNSTNFFIIAHSLTLTVFFVYLLCLYTSPQGKTSWLCLPPVINKLFVFLSIYSPIGRKPQQKNKELMLVEKNFIAIVSYRIGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCY7</accession>
    <name>D5GCY7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00000876001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430129">
        <property type="protein sequence ID" value="CAZ82380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838189.1">
        <property type="nucleotide sequence ID" value="XM_002838143.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCY7"/>
    <dbReference type="FunCoup" id="D5GCY7">
        <property type="interactions" value="82"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GCY7"/>
    <dbReference type="GeneID" id="9183842"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000876001"/>
    <dbReference type="eggNOG" id="ENOG502S47U">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064532_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCY7"/>
    <dbReference type="OMA" id="HAWTICS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051285">
        <property type="term" value="C:cell cortex of cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009571">
        <property type="entry name" value="SUR7/Rim9-like_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052413">
        <property type="entry name" value="SUR7_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28019">
        <property type="entry name" value="CELL MEMBRANE PROTEIN YLR413W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28019:SF7">
        <property type="entry name" value="SUR7 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06687">
        <property type="entry name" value="SUR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="187"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="230"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="264"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="277"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ82380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="288" mass="31790" checksum="A2462BAB830F61AF" modified="2010-06-15" version="1">MRFSAIVPIALSIAAFILSMLVLFAGRNNGFLGDTYVTKIDTSNLNVSNTGISALDNILDSAAQDIVNTTASRLGLHDFYKVFIMNYCEGERDSNDNERITNCTKQKGMFTFNPVGVFERELLQGVTLRDLGVNTQDLDTAINVLKVAYRTMFVTYCIGITAAGIAIILGFLGFIESRLIACSNWMFAVISFLFLGVASGIGTTTGVKIRDAINREMGGIGVRAYISKRYLGMTWAAVICMLIVTFFWCGACFSRRKNRTHHKSADENQLAGSGPVGKKKRSWRRWGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GD55</accession>
    <name>D5GD55_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ethanolamine-phosphate cytidylyltransferase</fullName>
            <ecNumber evidence="10">2.7.7.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">CTP:phosphoethanolamine cytidylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00000984001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytidylyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.14" evidence="10"/>
    <dbReference type="EMBL" id="FN430140">
        <property type="protein sequence ID" value="CAZ82448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838257.1">
        <property type="nucleotide sequence ID" value="XM_002838211.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GD55"/>
    <dbReference type="FunCoup" id="D5GD55">
        <property type="interactions" value="541"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GD55"/>
    <dbReference type="GeneID" id="9183912"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000984001"/>
    <dbReference type="eggNOG" id="KOG2803">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031246_0_0_1"/>
    <dbReference type="InParanoid" id="D5GD55"/>
    <dbReference type="OMA" id="QCKYINA"/>
    <dbReference type="UniPathway" id="UPA00558">
        <property type="reaction ID" value="UER00742"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004306">
        <property type="term" value="F:ethanolamine-phosphate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd02174">
        <property type="entry name" value="CCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02173">
        <property type="entry name" value="ECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041723">
        <property type="entry name" value="CCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044608">
        <property type="entry name" value="Ect1/PCYT2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45780">
        <property type="entry name" value="ETHANOLAMINE-PHOSPHATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45780:SF2">
        <property type="entry name" value="ETHANOLAMINE-PHOSPHATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="12">
        <location>
            <begin position="23"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Cytidyltransferase-like" evidence="12">
        <location>
            <begin position="227"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ82448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="392" mass="43712" checksum="A7DE34A7594AD85B" modified="2010-06-15" version="1">MADSLPEHPPNTPTPMSDDRIWIDGCFDFAHHGHAGAMLQAKQLGKYLVVGVHSDEEILENKGPTVMNLKERMAAVTACKWTDLPVPGAPYVTSPEWMDLYGTRYVVHGDDVTTDSSGEDCYRIVKAAGRFKVVKRTPGISTTDLVGRMLLCTKTHHIRDLGKLLLDQTSEEGRELLERTKMYASDSFGVVGKGSTVYNFVTEQPGGRCVPVVSGVGPKPAQRVVYVDGGFDLFSSGHIEFLRRVVEREKEEGHEDIYVVAGVHDDRTINQWKGLNYPIMNMFERGLCVLQCRYINSVVLTAPFSPTKGFLETLPTGVPSVVYHGPTTFMPGDEDPYEEAKEMGIFQEIGNHEFAGVNAGEIMGRILKQRELYEERQRRKGVKAENEVQMIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDV8</accession>
    <name>D5GDV8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00006298001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430153">
        <property type="protein sequence ID" value="CAZ82701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838510.1">
        <property type="nucleotide sequence ID" value="XM_002838464.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDV8"/>
    <dbReference type="STRING" id="656061.D5GDV8"/>
    <dbReference type="GeneID" id="9184496"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006298001"/>
    <dbReference type="HOGENOM" id="CLU_368890_0_0_1"/>
    <dbReference type="InParanoid" id="D5GDV8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRCT" evidence="2">
        <location>
            <begin position="625"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="225"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="232"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ82701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="755" mass="85881" checksum="FE0E4B9180D134F1" modified="2010-06-15" version="1">MNAFRPPSRRITSPAPSSSAWTLSSNRSTLSSFLDEEYGYYESMGGITSGYSELSMSSQLSSSSIEVHSYSGPSISPLYASTKSSAGGLRGPGSTTAGVKGNEQTGIPSDILPRLQVLLKNQVLVFLEGVEYDDLPLGAMLHKHSYKLAARNDEILEEVRQEGRAVTSEKAHLRYFKGLYRVIRRSQFSGVGGKLWGLWKLETVIAEAYRRSGIDPAIYGYPRLRSSGGHDDDMDDGEADLGSDEDGDDDLDFFDIQDETDDTDDPFDSDDTDDTDDMDHEEGHPMINAGGIGKGPVYGPQFERLSERSLTLLLDLLREFALNRYLLAAYKSILEHELSRPDEISELFAPKKLVNSPAAVRAVRVKEACIISYMQSLTFLYKLQLLVEYERKDPEGYWDKDFEHPDHEDEPLSLLWSKSMMTMVMTHFHREESDSERYRHRHGLYHGHYQEDWSYKLMYMTNYLHFAEATVHDWFADPDAQEWEEHEKQVKADDRCRWFSEFGGRLYLMPSTKMYGSRAAIVEWMEGFEEEQVLDTSVSELEAKALAGEYFRFVDMRPEWEDAATTLIKRYGGAIDIGQGDDDKPVTTILRPGQEDPVKCDRSVDYNHIMDYIHRKIVQTRFSHVDKKALKNVVFSFRGILPTLTRGDMRELVHIYGGFVEENLTSRIDYTVVGSRNPLDNPQVWSREMKIIEQLGIRKIDEDGFAELLLKSQSWLRNGKRKAANGSAVGEVFKPTSKRRRLATSLSLPSPDPAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>D5GE39</accession>
    <name>D5GE39_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00006364001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430164">
        <property type="protein sequence ID" value="CAZ82782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838591.1">
        <property type="nucleotide sequence ID" value="XM_002838545.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GE39"/>
    <dbReference type="FunCoup" id="D5GE39">
        <property type="interactions" value="687"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GE39"/>
    <dbReference type="GeneID" id="9183114"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006364001"/>
    <dbReference type="eggNOG" id="KOG0706">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023062_7_0_1"/>
    <dbReference type="InParanoid" id="D5GE39"/>
    <dbReference type="OMA" id="PANQVCF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048205">
        <property type="term" value="P:COPI coating of Golgi vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08831">
        <property type="entry name" value="ArfGap_ArfGap2_3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.150:FF:000013">
        <property type="entry name" value="Putative Arf GTPase-activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45686:SF4">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR GTPASE ACTIVATING PROTEIN 3, ISOFORM H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45686">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR GTPASE ACTIVATING PROTEIN 3, ISOFORM H-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Arf-GAP" evidence="7">
        <location>
            <begin position="10"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="151"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="269"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="207"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="288"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ82782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="464" mass="49604" checksum="55C246D93EDD272B" modified="2010-06-15" version="1">MSLASKTEGQKIFEKLKSNRANKVCFDCNSKNPTWSSVPFGIYLCLDCSAHHRNLGVHISFVRSTVLDQWQWDQLRLMKVGGNESATKYFQSHGGSAALASKDPKVKYGSNTATKYKEELKKRAAADAQLYPAEVIVEGGEEVAVPTEAEEDFFSSWDKPSIKRPTPPPSRTATPPVVGRTGTPSNGSGIARAKSPLASPETAPAVSRTTTSSALRTKAGATSASGPRRTGVLGAKKGQKLGAKKATGGDVIDFDEAEKKAKEEAERIARLGYDPEAEQQTKKETKASVLSPTPVTPSSPTHARQSSDVERLGLSMNKLGFGQVAGAAAASAPAKKTGGFGSVGLSSKVEEDDQKYAREKFGTQKAISSDEFFGRNTYDPQAVSEARTRLQGFEGATAISSNQYFGRDDEEHDADAAGYSELELTARDFARRFTGTAGEDLENAAQVLGQAAAKASEIVGRYMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>D5GE67</accession>
    <name>D5GE67_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DnaJ homolog 1, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00006401001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430164">
        <property type="protein sequence ID" value="CAZ82810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838619.1">
        <property type="nucleotide sequence ID" value="XM_002838573.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GE67"/>
    <dbReference type="FunCoup" id="D5GE67">
        <property type="interactions" value="823"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GE67"/>
    <dbReference type="GeneID" id="9183142"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006401001"/>
    <dbReference type="eggNOG" id="KOG0715">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017633_0_3_1"/>
    <dbReference type="InParanoid" id="D5GE67"/>
    <dbReference type="OMA" id="MATDYYA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006458">
        <property type="term" value="P:'de novo' protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10747">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000005">
        <property type="entry name" value="Chaperone protein dnaJ 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.230.10:FF:000001">
        <property type="entry name" value="DnaJ subfamily A member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.230.10">
        <property type="entry name" value="Heat shock protein DnaJ, cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01152">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012724">
        <property type="entry name" value="DnaJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43096">
        <property type="entry name" value="DNAJ HOMOLOG 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43096:SF52">
        <property type="entry name" value="DNAJ HOMOLOG 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00684">
        <property type="entry name" value="DnaJ_CXXCXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="DnaJ/Hsp40 cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="1 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 7" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="J" evidence="9">
        <location>
            <begin position="63"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="CR-type" evidence="10">
        <location>
            <begin position="202"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="7">
        <location>
            <begin position="202"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="33"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="442"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="470"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00072890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ82810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="489" mass="53375" checksum="748B9C26374D4A62" modified="2010-06-15" version="1">MSFIPPVLPRAVRVLRTSSNPITNFRCSYAPTLPRSLPVPRNSNHSPRRSFHSTPRSQASVKNPYSVLGLDKNASTSAIKKAYYSLAKKWHPDQNKDSSAREKFQEIQSAYEILSDPEKKEQFDQYGEAAFKPSAGFNPGTGAGFGQSGFREGFPADFSFEDLFSAFGGSSGRGTSGSEGGIPFGDNIEVETTISFMEAAKGVSKDIHIHAYLPCKTWAGTGMKSGTQRQPCGRCGGTGTRVHFMQGNFQMASTCDACKGAGVVIPRGSECGTCDGWGVTKEPRTITIDIPAGVDTGMRIKVDNEGDAPHSKASGASSTKTWRGDLFVHLRVAPHKYFRRKGLDVYYTATIPLTTALLGGRIRVPTLSGGEEDIEVPLGTTTGDTTKIYRKGMRGFDSVSVGNFTVEFKVNPPKTLTARQRILLEMLAEELDDKTARRIMGVNSEKQGPSPYEQHKNENFIKRAWHNLTHQHDDMPKDDDDQKKASGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEE1</accession>
    <name>D5GEE1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Mannosyltransferase</fullName>
            <ecNumber evidence="11">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00006440001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Endoplasmic reticulum membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the glycosyltransferase 22 family. PIGZ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="11"/>
    <dbReference type="EMBL" id="FN430175">
        <property type="protein sequence ID" value="CAZ82884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838693.1">
        <property type="nucleotide sequence ID" value="XM_002838647.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEE1"/>
    <dbReference type="FunCoup" id="D5GEE1">
        <property type="interactions" value="47"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GEE1"/>
    <dbReference type="GeneID" id="9185540"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006440001"/>
    <dbReference type="eggNOG" id="KOG4123">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022957_2_0_1"/>
    <dbReference type="InParanoid" id="D5GEE1"/>
    <dbReference type="OMA" id="HGIHPRY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005599">
        <property type="entry name" value="GPI_mannosylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760:SF3">
        <property type="entry name" value="GPI MANNOSYLTRANSFERASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03901">
        <property type="entry name" value="Glyco_transf_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072678" description="Mannosyltransferase" evidence="12">
        <location>
            <begin position="18"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="171"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="207"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="344"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ82884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="505" mass="56592" checksum="67C9D7C3BE24D88F" modified="2010-06-15" version="1">MWRRVYLVLLAVRAYFALSPSYIHPDEHFQGPEVIAGDIFDWRITKTWEFTSSAPIRSVFPLWAVYGIPMHLLHWIAPGSGPPSPWLVFNTLRVLFFLISFVLEDWALQELIASPRLRRTSLLLLASSFVTWTYQTHTFSNAVETIGVAWALVLIQRIKTSKEAAWLSSGLFGMTVVFCFFNRITFPAFILLPAFQLFPHFLRHPTCIIPLTLTTLLTVGAAVYYDTVFYSANTPTISLTSLFGTAPIITPINNLLYNASPENLSNHGLHPRWTHFLVNLPQLLGPATLCLVTIRRASLPVLSAISGISILSISPHQEARFLIPAVPLLLCSISLPRNKILNKLWITSWIIFNVLLGALFGVFHQGGVVPTQVYLGSLPLTPTPTTAVWWKTYSPPTWLLGAKDDSLITIDLMSSSLDKLVETVKPLADCSQENYLIAPMSSTALDQFVSINSPSQPFMLEKKWEYMNHFNLDDLDFADDGVLPTIQRVVGRRGLAVWRIGKIGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEH0</accession>
    <name>D5GEH0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00006480001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430175">
        <property type="protein sequence ID" value="CAZ82913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838722.1">
        <property type="nucleotide sequence ID" value="XM_002838676.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEH0"/>
    <dbReference type="STRING" id="656061.D5GEH0"/>
    <dbReference type="GeneID" id="9181544"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006480001"/>
    <dbReference type="eggNOG" id="KOG1118">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034817_1_0_1"/>
    <dbReference type="InParanoid" id="D5GEH0"/>
    <dbReference type="OMA" id="KSMTSYV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07593">
        <property type="entry name" value="BAR_MUG137_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03114">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00721">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51021">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BAR" evidence="2">
        <location>
            <begin position="14"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="234"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="273"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="346"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="367"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="380"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="416"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ82913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="447" mass="50264" checksum="F87513EB49B55BC7" modified="2010-06-15" version="1">MMNKKLDRLKQWAGERMGGEVKTGASDEFQNLEVEMKLRHEGAEKLHNTMNVYVKSVSQKSMGEDREKMLPVDVMAQAMISHGEEFESDSVFGNCLIKMGQANEKIARVQDSYVAAASESWLESLERFLTQMKEYQVARKKLESRRLAYDATLSKMQKQKKEDFRVEEELRAQRIKYEESSEDVYRRMGDIQESETETMADLTAFLDAELEYHDRCRDILVGIRQNWPAGLTAKDKKRLRSRSNPAHSFGNGSGIREESPPPLPQVERPSIRSRVNNTGGSPESNDRSGLFRLPTPDHNDRLSTKPAFHRTNTLPTPGRIGQYESESMGRRRSDQGGPPPLPATRTLRSVNTDVFSDNNSHTSARNSPDRERYRYEERSVSPARSATPSASRVPSRTSSNNALSSAYNAAGSGRKQPPPPPVSRGLKKPPPPPPPAKKSQLAAAYEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>D5GF89</accession>
    <name>D5GF89_TUBMM</name>
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        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00006782001</name>
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    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430208">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838991.1">
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GF89"/>
    <dbReference type="STRING" id="656061.D5GF89"/>
    <dbReference type="GeneID" id="9188038"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006782001"/>
    <dbReference type="eggNOG" id="ENOG502SJGS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010595_2_3_1"/>
    <dbReference type="InParanoid" id="D5GF89"/>
    <dbReference type="OMA" id="AWILMIT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591:SF10">
        <property type="entry name" value="ABC TRANSMEMBRANE TYPE-1 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13489">
        <property type="entry name" value="Methyltransf_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="14"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="372" mass="41850" checksum="21627ABFAA7C9A63" modified="2010-06-15" version="1">MASHDPEYSEYIAAEDSTSSGADDSDYASGSEDSTQSLLSNDITHTRKGHTLCLMMRHDPLTIYSPTKSVNCMITVFGNCPLLRDHRLNKTGMAGTSPSSMSKGESPELRILDMTHHLYLLMTGGSLFAAPLDEKNPPQRILDIGTGTGIWAVDIAEQFPSAEVIATDLSPIQPNWVPPNLRFEIDDAQDDWTWGDETFDYIHVRGLLGSIKDWDRLLRQAFTHLKPGGWLEAVEHDYVIRSDDGTYPEDCAIRRWFDLMNEAADKAGRTLSKIESINDSFRTVGFENVAETRHKVPWGTWPKERKKKELGAWILMITESGFESFGMALMTRTLGMTPSDVSVLCEKAFAELKSKKFHIYNYHHFLIGQKPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFE9</accession>
    <name>D5GFE9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00006861001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430208">
        <property type="protein sequence ID" value="CAZ83242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839051.1">
        <property type="nucleotide sequence ID" value="XM_002839005.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFE9"/>
    <dbReference type="GeneID" id="9188190"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006861001"/>
    <dbReference type="HOGENOM" id="CLU_2122863_0_0_1"/>
    <dbReference type="InParanoid" id="D5GFE9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ83242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="114" mass="12888" checksum="EACE5A9E6C164D55" modified="2010-06-15" version="1">MIQLLVQPNLTPITHSAKGLRPGPGSKYQDLISDWRTTTTNPGKIAPTRCSPLLRGEMSCFSFLIGYIQLEKLAMQDAKFWGEWNRCSDIRNMGYIIQVLPAAILAWHVLQRCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFJ6</accession>
    <name>D5GFJ6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00006938001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430209">
        <property type="protein sequence ID" value="CAZ83289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839098.1">
        <property type="nucleotide sequence ID" value="XM_002839052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFJ6"/>
    <dbReference type="STRING" id="656061.D5GFJ6"/>
    <dbReference type="GeneID" id="9188237"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006938001"/>
    <dbReference type="HOGENOM" id="CLU_2904680_0_0_1"/>
    <dbReference type="InParanoid" id="D5GFJ6"/>
    <dbReference type="OMA" id="ILLNHAQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ83289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="61" mass="7006" checksum="88C64A4E13AB8B94" modified="2010-06-15" version="1">MSEAYERERQNNARLDDLASKVTSLRDVTINIYDSARDHEIIDNSVHPPDSLLPPAVRWVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFQ2</accession>
    <name>D5GFQ2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00007005001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430220">
        <property type="protein sequence ID" value="CAZ83345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839154.1">
        <property type="nucleotide sequence ID" value="XM_002839108.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFQ2"/>
    <dbReference type="STRING" id="656061.D5GFQ2"/>
    <dbReference type="GeneID" id="9188293"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007005001"/>
    <dbReference type="eggNOG" id="KOG3026">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069491_1_0_1"/>
    <dbReference type="InParanoid" id="D5GFQ2"/>
    <dbReference type="OMA" id="CMAVWSQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd20446">
        <property type="entry name" value="Tudor_SpSPF30-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041297">
        <property type="entry name" value="Crb2_Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46297:SF2">
        <property type="entry name" value="TUDOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46297">
        <property type="entry name" value="ZINC FINGER CCCH-TYPE WITH G PATCH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18115">
        <property type="entry name" value="Tudor_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00333">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tudor" evidence="4">
        <location>
            <begin position="90"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="48"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="157"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="200"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="258"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="60"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="161"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="171"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ83345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="298" mass="32001" checksum="A78E84875B63051A" modified="2010-06-15" version="1">MEAELKEYKLQLESVEAALKADPENPELVGLVTELKEVIALTESVLAEQAGQAPSPPPSHHNRNHTNHNNKRDSPSTSNEVGGAGTGSNSIYAVGDNVLARWTSGDNQLYPARITSVMGSQQNPVYIVKFTQYNATETLHAQHIKPASNFASKKRKLDNSPIHSNGNNIGPSSASSTPTTSAISNSAIISQPATINTELAEASKRESSKAADGPSKVPKTGRKIAQKKVLEEGKKKWQDFASKGVKGKTGKARKIGESSMFRTPEGVHGRVGFTGSGQPMRKDVARGKHVYHQGEEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>D5GG54</accession>
    <name>D5GG54_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007202001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430242">
        <property type="protein sequence ID" value="CAZ83497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839306.1">
        <property type="nucleotide sequence ID" value="XM_002839260.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GG54"/>
    <dbReference type="STRING" id="656061.D5GG54"/>
    <dbReference type="GeneID" id="9184559"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007202001"/>
    <dbReference type="eggNOG" id="ENOG502S3TP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085417_1_0_1"/>
    <dbReference type="InParanoid" id="D5GG54"/>
    <dbReference type="OMA" id="FSWINRF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37852:SF1">
        <property type="entry name" value="HIG1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37852">
        <property type="entry name" value="YALI0B21208P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="174" mass="18698" checksum="A54AC03E0FABEBD2" modified="2010-06-15" version="1">MDSPLHTKSTPLPLSAKLPLTLLPSLMLGTTLGLALGAKRSALVFRAENAHRIPRTTRGWYFYHKSKNYYTMLGAAREGMRSGVRVAGWVGGFVLAGGVVGEVLGPLGGGVVAGLSVAGVFSWINRFSYGMAVTTAKRGLFFGFLFGAGEEAIGYIGRKKREIEEQKGWEGTSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5GG71</accession>
    <name>D5GG71_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007241001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430242">
        <property type="protein sequence ID" value="CAZ83514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839323.1">
        <property type="nucleotide sequence ID" value="XM_002839277.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GG71"/>
    <dbReference type="STRING" id="656061.D5GG71"/>
    <dbReference type="GeneID" id="9184576"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007241001"/>
    <dbReference type="HOGENOM" id="CLU_3108139_0_0_1"/>
    <dbReference type="InParanoid" id="D5GG71"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH myb-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="51" mass="5658" checksum="E227A96A96EB6BEA" modified="2010-06-15" version="1">MHYAEFGEDESAALLAAIKEYEANKWKVIGTKVGKPAKACEQYAKEHFAGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5GG93</accession>
    <name>D5GG93_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007269001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430253">
        <property type="protein sequence ID" value="CAZ83536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839345.1">
        <property type="nucleotide sequence ID" value="XM_002839299.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GG93"/>
    <dbReference type="GeneID" id="9184598"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007269001"/>
    <dbReference type="HOGENOM" id="CLU_1409751_0_0_1"/>
    <dbReference type="InParanoid" id="D5GG93"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072078" evidence="1">
        <location>
            <begin position="22"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="193" mass="21827" checksum="C915EBCD5A862324" modified="2010-06-15" version="1">MSRYFRLPSLHLLSLCSVIRFLNLRSYFYFVQNACTPGNRSDGDASELPAHDQNLGSSETLVLPVITHITNPLCVWLRSWLRRENLFYGSGHDKSTHPAIVQPALEREELTAESRDVEKLFLEAASYLPIPPDTYPISKHYHGLNTASQNLHYGRKLPTGNDLSGNPTGKWRPTRAITVAAPKLRGPLHKRNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGL2</accession>
    <name>D5GGL2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 12">Nicotinate-nucleotide pyrophosphorylase [carboxylating]</fullName>
            <ecNumber evidence="5 12">2.4.2.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 12">Quinolinate phosphoribosyltransferase [decarboxylating]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">GSTUM_00007545001</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 12">Involved in the catabolism of quinolinic acid (QA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>nicotinate beta-D-ribonucleotide + CO2 + diphosphate = quinolinate + 5-phospho-alpha-D-ribose 1-diphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:12733"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:29959"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from quinolinate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 12">Hexamer formed by 3 homodimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the NadC/ModD family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.19" evidence="5 12"/>
    <dbReference type="EMBL" id="FN430275">
        <property type="protein sequence ID" value="CAZ83742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839551.1">
        <property type="nucleotide sequence ID" value="XM_002839505.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGL2"/>
    <dbReference type="FunCoup" id="D5GGL2">
        <property type="interactions" value="212"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GGL2"/>
    <dbReference type="GeneID" id="9181712"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007545001"/>
    <dbReference type="eggNOG" id="KOG3008">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039622_1_0_1"/>
    <dbReference type="InParanoid" id="D5GGL2"/>
    <dbReference type="OMA" id="DIVMCDN"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00331"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004514">
        <property type="term" value="F:nicotinate-nucleotide diphosphorylase (carboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034213">
        <property type="term" value="P:quinolinate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000090">
        <property type="entry name" value="Nicotinate-nucleotide pyrophosphorylase [carboxylating]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1170.20:FF:000003">
        <property type="entry name" value="Nicotinate-nucleotide pyrophosphorylase [carboxylating]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.20">
        <property type="entry name" value="Quinolinate phosphoribosyl transferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004393">
        <property type="entry name" value="NadC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027277">
        <property type="entry name" value="NadC/ModD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036068">
        <property type="entry name" value="Nicotinate_pribotase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037128">
        <property type="entry name" value="Quinolinate_PRibosylTase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002638">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022412">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00078">
        <property type="entry name" value="nadC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32179">
        <property type="entry name" value="NICOTINATE-NUCLEOTIDE PYROPHOSPHORYLASE [CARBOXYLATING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32179:SF3">
        <property type="entry name" value="NICOTINATE-NUCLEOTIDE PYROPHOSPHORYLASE [CARBOXYLATING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01729">
        <property type="entry name" value="QRPTase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02749">
        <property type="entry name" value="QRPTase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006250">
        <property type="entry name" value="NadC_ModD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51690">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54675">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 12" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Quinolinate phosphoribosyl transferase N-terminal" evidence="14">
        <location>
            <begin position="43"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Quinolinate phosphoribosyl transferase C-terminal" evidence="13">
        <location>
            <begin position="118"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00020990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR006250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAZ83742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="284" mass="30748" checksum="4C6EABE572AF24C7" modified="2010-06-15" version="1">MAPLEHGNTAHLLPNTYKQIIASWLTEDTPTFDYGGFVVGEDPKTATLYAKSPGVLAGVPFFDEVFTQLGCSVEWHAQEGTFLPAAKPIAIATVKGPSRNLLLGERVALNTLARCSGIATKSRRLLELVRKAGYSGILAGTRKTTPGFRLVEKYGMLVGGVDQHRYDLSSMIMLKDNHIWSKGSITKAVLAAKSVGGFVVKVEVEVQSEEEADEAIAAGADIVMLDNFTGDGLKASAANLKKRWAEKGRQDNVEGYLCNDIDIISTSSIHQSTQHVDFSLKIDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGM3</accession>
    <name>D5GGM3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007422001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430275">
        <property type="protein sequence ID" value="CAZ83645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839454.1">
        <property type="nucleotide sequence ID" value="XM_002839408.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGM3"/>
    <dbReference type="STRING" id="656061.D5GGM3"/>
    <dbReference type="GeneID" id="9185567"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007422001"/>
    <dbReference type="eggNOG" id="ENOG502SD1F">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_797380_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGM3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="200"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="348" mass="38444" checksum="3B1FB9A7E8D16048" modified="2010-06-15" version="1">MAAIQADSLSPIVDLADHPPQYINPDAPVLSSLMLYIVRVPGSQDLFLTTLHPLQSTVTAEDVNACLYYIHVEPVVNTSSISPSTLSPPLPRPPSHRRWSSQSITRSRHPSRSPARPIPTMTIVRRDPATGAQWNVARISSPSNAEEDMQRILVDIMTAGYQQFVEPTVDPDELVSIGFRREMWMEGAGFWSRVKNIGHKRGRSTSTSSDPREKESAAATPVGEKMKKRGYVFDALWSPGKGEERGRCGFKDEAGGKFLKCKYYPPTRSSKSSLLSVLEFKPPSTTVLSAAPGREAPEVITSSKSKLGMLVCHSDGLYMMDLLVAANMAVFWKRWESWALERSALGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGP5</accession>
    <name>D5GGP5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">Altered inheritance of mitochondria protein 24, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00007454001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the AIM24 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430275">
        <property type="protein sequence ID" value="CAZ83667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002839430.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGP5"/>
    <dbReference type="FunCoup" id="D5GGP5">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="GeneID" id="9185589"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007454001"/>
    <dbReference type="eggNOG" id="ENOG502RXC5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046558_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGP5"/>
    <dbReference type="OMA" id="QGPRTIL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007007">
        <property type="term" value="P:inner mitochondrial membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.160.10">
        <property type="entry name" value="Mitochondrial biogenesis AIM24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002838">
        <property type="entry name" value="AIM24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036983">
        <property type="entry name" value="AIM24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016031">
        <property type="entry name" value="Trp_RNA-bd_attenuator-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36959">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 24, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36959:SF2">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 24, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AIM24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51219">
        <property type="entry name" value="TRAP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="374"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="383"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ83667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="427" mass="46288" checksum="4180E8E2F66E9624" modified="2010-06-15" version="1">MAARRLNPSFIPMARSFLSTPSVLARLGPCSNRYVHIRATPSTTALDGFLDLSEQTQSIPKLSGAGPFPAGMLAGGAGYPLFGRGGLIDSMRFLDVKFEVLGAPYSLLSVALPASSVLYSRRGTLVGVNGKAGNAVSTLSLLEPIRRSFLRIPFLYQKISSTSPLTCLISTNSPNTTFGVIELDGKADWMVTQNDALLAWTGHSISAKPSVSSRMSLAHWGNSKLTGRGLVALVGRGQIYQVTLKAGEEFTVHPGSLLAYSISTAFRPMPYRLRSASMRLQVPRLNVDRLLPQIGFIRVMRNSEVSKTITKAFFVFRAWLRRTIWGDRLFVRFRGPGTVLLQSRSARISDILRPHDVSEAATVEPGALEPLEHASGGAVGSVPKPSSKPLLPASSSAMDHNGLKVVTVKKDGKVEFEDSDFRDFARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGX6</accession>
    <name>D5GGX6_TUBMM</name>
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            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007601001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430297">
        <property type="protein sequence ID" value="CAZ83769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839578.1">
        <property type="nucleotide sequence ID" value="XM_002839532.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGX6"/>
    <dbReference type="GeneID" id="9185100"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007601001"/>
    <dbReference type="HOGENOM" id="CLU_2795789_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGX6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="68" mass="7698" checksum="517E45B09C1F096E" modified="2010-06-15" version="1">MNYPADSLGPLPLTRRSHGEQPLDHRYLAKTTVRIHVGPSPEAAEVYAPYPPPWKKQRLTVKSHKRGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGY4</accession>
    <name>D5GGY4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00007618001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430297">
        <property type="protein sequence ID" value="CAZ83777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839586.1">
        <property type="nucleotide sequence ID" value="XM_002839540.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGY4"/>
    <dbReference type="GeneID" id="9185031"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007618001"/>
    <dbReference type="HOGENOM" id="CLU_2689596_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGY4"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ83777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="74" mass="8561" checksum="7D37152F93892AD1" modified="2010-06-15" version="1">MWPERKNDNDKTRAMPPPVPCRLSLSRLWASSRAETAPYQSRCLSNEPVAVMFKTDKCMIKREVNHSIKSRALE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>D5GH26</accession>
    <name>D5GH26_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00007670001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430297">
        <property type="protein sequence ID" value="CAZ83819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839628.1">
        <property type="nucleotide sequence ID" value="XM_002839582.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GH26"/>
    <dbReference type="STRING" id="656061.D5GH26"/>
    <dbReference type="GeneID" id="9185073"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007670001"/>
    <dbReference type="eggNOG" id="ENOG502QX35">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029790_3_0_1"/>
    <dbReference type="InParanoid" id="D5GH26"/>
    <dbReference type="OMA" id="WTGRNCL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044669">
        <property type="entry name" value="YneE/VCCN1/2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33281:SF16">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33281">
        <property type="entry name" value="UPF0187 PROTEIN YNEE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25539">
        <property type="entry name" value="Bestrophin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="40"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ83819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="327" mass="36873" checksum="69DA225D86BB3D4D" modified="2010-06-15" version="1">MSPLPTHTNGVGPRATFPLSKSLRQKPRKWPLVFRFIKGAIHYVILIPVLFHTLFTIAVLLVHRLVDVDLALPSSIVPSLSIVVGLMLVFRNSSSYERYWGGRTALQQIVNNLRNLVRTFLVCGPLSDDSDRVETERTVKTLVAMLYAVKNHLRGNWEASALQPEFASLMPEGFRGHEAEGVGLPMELAFVVERYIKRGVKKGAFNAPQSANLNASLNAVIMAYGNMETIKLTPIPVCQQIHQKQVLALYCCVLPFCLLDDMGWWTIPVITLVCFTLYGIEGIGEELEDPFGNDRNDIKIDAIIEDCRQEVMVLLESWKRGREEYYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHJ8</accession>
    <name>D5GHJ8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Multifunctional tryptophan biosynthesis protein</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="16">Anthranilate synthase component 2</fullName>
                <shortName evidence="16">AS</shortName>
                <ecNumber evidence="16">4.1.3.27</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="16">Anthranilate synthase, glutamine amidotransferase component</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="16">Indole-3-glycerol phosphate synthase</fullName>
                <shortName evidence="16">IGPS</shortName>
                <ecNumber evidence="16">4.1.1.48</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="16">N-(5'-phosphoribosyl)anthranilate isomerase</fullName>
                <shortName evidence="16">PRAI</shortName>
                <ecNumber evidence="16">5.3.1.24</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="20" type="ORF">GSTUM_00007952001</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 16">Trifunctional enzyme bearing the Gln amidotransferase (GATase) domain of anthranilate synthase, indole-glycerolphosphate synthase, and phosphoribosylanthranilate isomerase activities.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate + H(+) = (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:23476"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58613"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="EC" id="4.1.1.48"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 16">
            <text>N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:21540"/>
            <dbReference type="ChEBI" id="CHEBI:18277"/>
            <dbReference type="ChEBI" id="CHEBI:58613"/>
            <dbReference type="EC" id="5.3.1.24"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 16">
            <text>chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21732"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="4.1.3.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6 16">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 16">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 3/5.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5 16">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 4/5.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.27" evidence="16"/>
    <dbReference type="EC" id="4.1.1.48" evidence="16"/>
    <dbReference type="EC" id="5.3.1.24" evidence="16"/>
    <dbReference type="EMBL" id="FN430319">
        <property type="protein sequence ID" value="CAZ83991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839800.1">
        <property type="nucleotide sequence ID" value="XM_002839754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHJ8"/>
    <dbReference type="FunCoup" id="D5GHJ8">
        <property type="interactions" value="358"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GHJ8"/>
    <dbReference type="MEROPS" id="C26.959"/>
    <dbReference type="GeneID" id="9183645"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007952001"/>
    <dbReference type="eggNOG" id="KOG0026">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4201">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4202">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007713_2_0_1"/>
    <dbReference type="InParanoid" id="D5GHJ8"/>
    <dbReference type="OMA" id="EPIEWAN"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00040"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004049">
        <property type="term" value="F:anthranilate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004425">
        <property type="term" value="F:indole-3-glycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004640">
        <property type="term" value="F:phosphoribosylanthranilate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01743">
        <property type="entry name" value="GATase1_Anthranilate_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00331">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00405">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000136">
        <property type="entry name" value="Multifunctional tryptophan biosynthesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000031">
        <property type="entry name" value="Multifunctional tryptophan biosynthesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00135">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050472">
        <property type="entry name" value="Anth_synth/Amidotransfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016302">
        <property type="entry name" value="Anthranilate_synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013798">
        <property type="entry name" value="Indole-3-glycerol_P_synth_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001468">
        <property type="entry name" value="Indole-3-GlycerolPSynthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001240">
        <property type="entry name" value="PRAI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006221">
        <property type="entry name" value="TrpG/PapA_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00566">
        <property type="entry name" value="trpG_papA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43418:SF4">
        <property type="entry name" value="MULTIFUNCTIONAL TRYPTOPHAN BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43418">
        <property type="entry name" value="MULTIFUNCTIONAL TRYPTOPHAN BIOSYNTHESIS PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00218">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00697">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001382">
        <property type="entry name" value="TrpG-trpC-trpF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00097">
        <property type="entry name" value="ANTSNTHASEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00096">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00614">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 16" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11 16" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="8 16" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="10" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="12 16" id="KW-0413">Isomerase</keyword>
    <keyword evidence="13 16" id="KW-0456">Lyase</keyword>
    <keyword evidence="14" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 16" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Glutamine amidotransferase" evidence="17">
        <location>
            <begin position="19"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Indole-3-glycerol phosphate synthase" evidence="18">
        <location>
            <begin position="237"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="domain" description="N-(5'phosphoribosyl) anthranilate isomerase (PRAI)" evidence="19">
        <location>
            <begin position="587"/>
            <end position="753"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR001382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CAZ83991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="760" mass="82216" checksum="1783ED4B1C4BE814" modified="2010-06-15" version="1">MSNPGSAQPALSTASNVIMIDNYDSFTWNIYQFLTLEGAKVTVYRNDKITLPELIALSPTQIVISPGPGHPITDSGVSIECVKHFAGKIPILGVCMGQQAIFSAFGGTVDYAGEVVHGKTSLIKHDTKGIYRNIPQDIAVTRYHSLAGTHATLPADLEVTSRTDRDVIMGIRHKEYAIEAVQYHPESILTEEGRMMFRNFLEMTAGTWKGCEVTLNTGSLENTTPDPNSAAKSESILDKIYAHRRAVVAAQKLLPSQRPADLEVLHSLSISPPLINFPARLRAVSTPCALMAEIKRASPSKGSIDMDASAAVQARTYALASAAAISVLTEPEWFKGSIDDLRAARQAVDGMPNRPAILRKEFIFEEYQILEARLAGADTVLLIVKMLSDEELKRLYDYSKSLGMEPLVEVNSREEMERALKVGSTVIGVNNRDLHSFKVDLETTSGLVGMIDKEKVVLCALSGISAREDVKRYEIEGVGAVLVGESLMRAGKNVKTFINELLTDSPTPTSAPHKHGVLVKICGTRSVEAAKVAVESGADMVGMILAEGTKRTIDPETAKAISDVVRSTPKGGVSDTPIVPKFAANPPHSCPKISAEGWFEHSVKNLIHHPKRSLLVGVFRNQPFSAVLALQQALALDIIQLHGSEPLEWARLLPVPVIRRFSPGEDGLNKSGYHAIPLLDSGIGGTGDRVDLSEVENVLAAGVPVMLAGGLNHHNVEEIRIGGLLEKGIIGVDVSSGIETDGRQDLIKIRRFVYRAKGRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHQ6</accession>
    <name>D5GHQ6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="11">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00007995001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PurH family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430320">
        <property type="protein sequence ID" value="CAZ84017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839826.1">
        <property type="nucleotide sequence ID" value="XM_002839780.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHQ6"/>
    <dbReference type="FunCoup" id="D5GHQ6">
        <property type="interactions" value="975"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GHQ6"/>
    <dbReference type="GeneID" id="9183671"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007995001"/>
    <dbReference type="eggNOG" id="KOG2555">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016316_3_2_1"/>
    <dbReference type="InParanoid" id="D5GHQ6"/>
    <dbReference type="OMA" id="IKHNNPC"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00133"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003937">
        <property type="term" value="F:IMP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004643">
        <property type="term" value="F:phosphoribosylaminoimidazolecarboxamide formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01421">
        <property type="entry name" value="IMPCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1380:FF:000003">
        <property type="entry name" value="Bifunctional purine biosynthesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.440:FF:000001">
        <property type="entry name" value="Bifunctional purine biosynthesis protein PURH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.440">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1380">
        <property type="entry name" value="Methylglyoxal synthase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024051">
        <property type="entry name" value="AICAR_Tfase_dup_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024050">
        <property type="entry name" value="AICAR_Tfase_insert_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011607">
        <property type="entry name" value="MGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036914">
        <property type="entry name" value="MGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002695">
        <property type="entry name" value="PurH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11692:SF0">
        <property type="entry name" value="BIFUNCTIONAL PURINE BIOSYNTHESIS PROTEIN ATIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11692">
        <property type="entry name" value="BIFUNCTIONAL PURINE BIOSYNTHESIS PROTEIN PURH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01808">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02142">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000414">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00798">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00851">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52335">
        <property type="entry name" value="Methylglyoxal synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51855">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MGS-like" evidence="10">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ84017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="541" mass="59043" checksum="ECDEF298E6EC213F" modified="2010-06-15" version="1">MSSSQKTAILSVYNKTGLLDLANGLALQGIRLLASGGTAKLIREAGIGVEDVSSITHAPEMLAGRVKTLHPAVHAGILARDLESDEKDLAEQNIDKVDYVVCNLYPFKDTIAKVNVTIAEAIEEIDIGGVTLLRAAAKNHSRVTILSDPADYPSFLEELRSGEVSEASRKLYALKAFEHTADYDATIAEFFRKRYAGDGVQHLTLRYGANPHQKPAQAFVESGKIPFKVLSGSPGYINLLDALNSWPLVKELKESLDLPAAASFKHVSPAGAAGLDDSPLACAYARARGADRMSSFGDWIALSDEVDAVTARIIGREVSDGVIAPAYTPGATLFFRLIPTMSPQALKPANSDYKLPPQALIDLTVATIALKYTQSNSVCYAKNGQIVGLGAGQQSRIHCTRLAGDKADNWWFRQHPRVLDFKWKKGTKRPDKSNAIDLFVSGLVPKKVEGDPEREQYESVFEEVPTDFTPEEREEWIKQCTNVSVSSDAFFPFTDNVFRAHRSGVKYLAAPGGSVMDQAVHEAADKCGMVFVHQETRLFHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHS7</accession>
    <name>D5GHS7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00008018001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS19 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430320">
        <property type="protein sequence ID" value="CAZ84038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839847.1">
        <property type="nucleotide sequence ID" value="XM_002839801.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHS7"/>
    <dbReference type="FunCoup" id="D5GHS7">
        <property type="interactions" value="760"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GHS7"/>
    <dbReference type="GeneID" id="9183692"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008018001"/>
    <dbReference type="eggNOG" id="KOG3411">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108559_0_0_1"/>
    <dbReference type="InParanoid" id="D5GHS7"/>
    <dbReference type="OMA" id="WAPFVKT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000118">
        <property type="entry name" value="40S ribosomal protein S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001266">
        <property type="entry name" value="Ribosomal_eS19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018277">
        <property type="entry name" value="Ribosomal_eS19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11710">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11710:SF0">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01090">
        <property type="entry name" value="Ribosomal_S19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01413">
        <property type="entry name" value="Ribosomal_S19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00628">
        <property type="entry name" value="RIBOSOMAL_S19E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="76"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="118"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="76"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="91"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="140"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ84038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="152" mass="16856" checksum="9A5334FADCFB4D64" modified="2010-06-15" version="1">MPGVSVRDVDAQKFIDAYAAFLKRQGKLQIPGWVDTVKTGHMKELPPQSIDWFYIRAAAVARHVYLRKSVGVGRLRKAHGGQKNRGSRPSHHVDASGSVDRKVLQALEKIGVVEISPKGGRRISQTGQRDLDRIAQTTVAEDEEGEDEDDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>D5GI89</accession>
    <name>D5GI89_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 16">Pyruvate kinase</fullName>
            <ecNumber evidence="5 16">2.7.1.40</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">GSTUM_00008343001</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15 16">
            <text>pyruvate + ATP = phosphoenolpyruvate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18157"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.40"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 16">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the pyruvate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.40" evidence="5 16"/>
    <dbReference type="EMBL" id="FN430324">
        <property type="protein sequence ID" value="CAZ84232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840041.1">
        <property type="nucleotide sequence ID" value="XM_002839995.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GI89"/>
    <dbReference type="FunCoup" id="D5GI89">
        <property type="interactions" value="896"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GI89"/>
    <dbReference type="GeneID" id="9181950"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008343001"/>
    <dbReference type="eggNOG" id="KOG2323">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015439_0_1_1"/>
    <dbReference type="InParanoid" id="D5GI89"/>
    <dbReference type="OMA" id="RVHHIGE"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00188"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030955">
        <property type="term" value="F:potassium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004743">
        <property type="term" value="F:pyruvate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061621">
        <property type="term" value="P:canonical glycolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00288">
        <property type="entry name" value="Pyruvate_Kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.60:FF:000001">
        <property type="entry name" value="Pyruvate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1380.20:FF:000001">
        <property type="entry name" value="Pyruvate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.33.10">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1380.20">
        <property type="entry name" value="Pyruvate kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001697">
        <property type="entry name" value="Pyr_Knase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018209">
        <property type="entry name" value="Pyrv_Knase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015793">
        <property type="entry name" value="Pyrv_Knase_brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015795">
        <property type="entry name" value="Pyrv_Knase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036918">
        <property type="entry name" value="Pyrv_Knase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015806">
        <property type="entry name" value="Pyrv_Knase_insert_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004491">
        <property type="entry name" value="PRK05826.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01064">
        <property type="entry name" value="pyruv_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11817">
        <property type="entry name" value="PYRUVATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00224">
        <property type="entry name" value="PK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02887">
        <property type="entry name" value="PK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01050">
        <property type="entry name" value="PYRUVTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52935">
        <property type="entry name" value="PK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00110">
        <property type="entry name" value="PYRUVATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13 16" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="10 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate kinase barrel" evidence="17">
        <location>
            <begin position="27"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate kinase C-terminal" evidence="18">
        <location>
            <begin position="370"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAZ84232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="496" mass="54714" checksum="BA29FC584EE4EE46" modified="2010-06-15" version="1">MTDSVKTQYQWLSQLNVDHVPAERNFRRTSIICTIGPKTNSVESINKLRKAGLNIVRMNFSHGSYEYHQSVIDNSREAERQHPGRPLAIALDTKGPEIRTGNTKGDADLPISAGSEIIITTDDKYVTSCTEKIMYVDYKNITKVLDVTDERNIKARCLNNGKISSKKGVNLPKTDVDLPPLSEKDKEDLRFGVKNGVHMVFASFIRRGSDITAIREVLGEDGKEIQIIAKIENQQGVNNFDEILEETDGVMVARGDLGIEIPPAQVFIAQKMMIAKCNLAGKPIICATQMLESMTYNPRPTRAEVSDVGNAVLDGADCVMLSGETAKGNYPIESVTMMHETCLLAETAISYVPLFNELRGLTKRPTETSETCAIAAVSASVEQEASAIIVLSTSGSTARLISKYRPSCPVLMITRNANAARYSHLYRGVYPFVYPKPKPDFNQVIWQEDVDERLRWGTSEAIKLGLVKAGDTIIAVQGWRGGLGNTNTLRVLNAKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>D5GID5</accession>
    <name>D5GID5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Serine/threonine-protein kinase</fullName>
            <ecNumber evidence="7">2.7.11.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00008436001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.21"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.21" evidence="7"/>
    <dbReference type="EMBL" id="FN430326">
        <property type="protein sequence ID" value="CAZ84278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840087.1">
        <property type="nucleotide sequence ID" value="XM_002840041.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GID5"/>
    <dbReference type="FunCoup" id="D5GID5">
        <property type="interactions" value="280"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GID5"/>
    <dbReference type="GeneID" id="9184636"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008436001"/>
    <dbReference type="eggNOG" id="KOG0575">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_46_2_1"/>
    <dbReference type="InParanoid" id="D5GID5"/>
    <dbReference type="OMA" id="FEECVTA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090619">
        <property type="term" value="C:meiotic spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035974">
        <property type="term" value="C:meiotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990023">
        <property type="term" value="C:mitotic spindle midzone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140602">
        <property type="term" value="C:nucleolar peripheral inclusion body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019237">
        <property type="term" value="F:centromeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051219">
        <property type="term" value="F:phosphoprotein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010458">
        <property type="term" value="P:exit from mitosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000086">
        <property type="term" value="P:G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031619">
        <property type="term" value="P:homologous chromosome orientation in meiotic metaphase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990571">
        <property type="term" value="P:meiotic centromere clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990813">
        <property type="term" value="P:meiotic centromeric cohesion protection in anaphase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090306">
        <property type="term" value="P:meiotic spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990395">
        <property type="term" value="P:meiotic spindle pole body organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904750">
        <property type="term" value="P:negative regulation of protein localization to nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045793">
        <property type="term" value="P:positive regulation of cell size"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010971">
        <property type="term" value="P:positive regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140281">
        <property type="term" value="P:positive regulation of mitotic division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045842">
        <property type="term" value="P:positive regulation of mitotic metaphase/anaphase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140429">
        <property type="term" value="P:positive regulation of mitotic sister chromatid biorientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010696">
        <property type="term" value="P:positive regulation of mitotic spindle pole body separation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046827">
        <property type="term" value="P:positive regulation of protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110083">
        <property type="term" value="P:positive regulation of protein localization to cell division site involved in mitotic actomyosin contractile ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035025">
        <property type="term" value="P:positive regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031031">
        <property type="term" value="P:positive regulation of septation initiation signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903353">
        <property type="term" value="P:regulation of nucleus organization"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902542">
        <property type="term" value="P:regulation of protein localization to mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051455">
        <property type="term" value="P:spindle attachment to meiosis I kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070194">
        <property type="term" value="P:synaptonemal complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd13118">
        <property type="entry name" value="POLO_box_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14099">
        <property type="entry name" value="STKc_PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.30">
        <property type="entry name" value="POLO box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033701">
        <property type="entry name" value="POLO_box_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000959">
        <property type="entry name" value="POLO_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036947">
        <property type="entry name" value="POLO_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345:SF0">
        <property type="entry name" value="CELL CYCLE SERINE_THREONINE-PROTEIN KINASE CDC5_MSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00659">
        <property type="entry name" value="POLO_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82615">
        <property type="entry name" value="Polo-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50078">
        <property type="entry name" value="POLO_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="105"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="POLO box" evidence="10">
        <location>
            <begin position="478"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="378"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="379"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ84278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="631" mass="70351" checksum="2D7B9B39D69840BA" modified="2010-06-15" version="1">MAALQKSALSSMDPNLPTRPLARKRSARATNPSPLRKQSDYQLVAPLKTAPTVASRPPPAPSSTANQKQLGDPKTPRVAVKDRKKKLLCATPPQVLQDTRGRAEYDRGRQLGEGGFARCFLVQNKEGELFAAKTVAKKSLQNQRMKAKFFGEIQVHKTMIHPNVVKFVECFEDSDNIYMILELCPNKSLMDMLRARKRFTEPETRFFLLQLLGALKYMHGKKVIHRDLKLGNIFLDADMNVKIGDFGLAALLINENDRKRTICGTPNYIAPEVLFGSGKEGQGHSFEVDLWGVGVIMYAMLVGKPPFQAGDVNVIYQKIRHNTFSWPEEVPVSSAAKHLVHSILNHDPDARPTLDDIADHYFFKSGFFPRSIPVGAGKQPPSLISTRSSSQQSSQPSSQSSSQPSEGPIIPFGPNLSSKIGVPIRSTATSTVLSSLRSFINSLEAFASSRLHTLSPIPAATIAAVKSSRRGESNKHVFITKWVDYTNKYGVAYILTDGTCTAMFNDNTSFVVDSISGERVEFITQSYISDSSQDTVYRRLQTKMEVLNEKKRTSKGLARKMQMWKRFKNYMKSDLETVEQWCVERDSVIREPTVGGGDDPGMLFVTHYTRLKRCALFRFSDGSFQVCCSNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIV9</accession>
    <name>D5GIV9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="20">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">GSTUM_00008707001</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S8 family. Furin subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840261.1">
        <property type="nucleotide sequence ID" value="XM_002840215.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIV9"/>
    <dbReference type="FunCoup" id="D5GIV9">
        <property type="interactions" value="142"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GIV9"/>
    <dbReference type="MEROPS" id="S08.070"/>
    <dbReference type="GeneID" id="9185670"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008707001"/>
    <dbReference type="eggNOG" id="KOG3525">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002976_2_1_1"/>
    <dbReference type="InParanoid" id="D5GIV9"/>
    <dbReference type="OMA" id="PWMHVKH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007323">
        <property type="term" value="P:peptide pheromone maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04059">
        <property type="entry name" value="Peptidases_S8_Protein_convertases_Kexins_Furin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000026">
        <property type="entry name" value="proprotein convertase subtilisin/kexin type 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000005">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034182">
        <property type="entry name" value="Kexin/furin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002884">
        <property type="entry name" value="P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884:SF14">
        <property type="entry name" value="NEUROENDOCRINE CONVERTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01483">
        <property type="entry name" value="P_proprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51829">
        <property type="entry name" value="P_HOMO_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 14 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 14 15" id="KW-0645">Protease</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14 15" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5 18" id="KW-0732">Signal</keyword>
    <keyword evidence="4 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072820" evidence="18">
        <location>
            <begin position="24"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="744"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="domain" description="P/Homo B" evidence="19">
        <location>
            <begin position="490"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="630"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="818"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="648"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="676"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="689"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="818"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="847"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="13 14">
        <location>
            <position position="204"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="13 14">
        <location>
            <position position="242"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="13 14">
        <location>
            <position position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CAZ84452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="863" mass="93118" checksum="B11BD23DB963DD01" modified="2010-06-15" version="1">MRFGSAPLWLAVLQIIPFPTVSAKSPPLLRPRDYDSRDYYALHLTPDVSPKTVALQLGLTHEGVIGELSDHHLFSTPTSKEDVVAIHIDGLRRKRRSVGSGGIVKRDLSDGILFAEKQRPKKLVKRVPPPAARREAQGFDPAAITGNTGVVELQSIMQRLDIRDPIFHEQWHLFNPIERHHDVNVTGLWLEGITGENTTVAIVDDGLDMDSQDLAANYFAAGSYDFNDHAPQPKPRLSDDRHGTRCAGEIAAVRNNVCGVGVAYDAKVAGIRMLSKPISDADEAIALNYAYHENQIYSCSWGPPDDGQAMDAPGILIKRAMVKGVQEGRNGKGSIFVFASGNGATYEDNCNFDGYTNSIYSITIGAIDRNGNHPYYSEQCSANMAVTYSSGSGDAIHTTDIGPNQCFNGHGGTSAAAPLAAGIFALVLSVRPGLTWRDMQYLALETAVPINEEDPDWKQTAIGKMFNHKYGYGKVDAWAIVHAAKDHKLVKPQAWYNSPIVIVNAAIPEGTQGLQTQIKVTAEEARNANLGRLEHVTVTMNAAHSQRGDMSVDLISPKGIVSHIATTRKPDTSKNGYKDWTFMSVKHWGEEATGTWTIIIKDTVVNGQTGSLTDWSLTLWGEAIDADKAKPLPLPGGDGDKNLGTDPIHTTTAQASTTELPSMTSTANPSKVTENPSDHADRPVNEKPGSAATSTQLGTGTTTGTTTATPTSTAAPGGPEAEAEESNFLPSFFPTFGVSAKTQVWIYGAFVVIILFVGVLATYLSIQKKRAKKQGMDYEFEALNNEDDLESGAAGAGGKASAGGRRKARDLYDAFGASDDEEDILSEKEYDDDEGSYDDDEALGIDEGPRVGDRAKLLGRDNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJ85</accession>
    <name>D5GJ85_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00008881001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840387.1">
        <property type="nucleotide sequence ID" value="XM_002840341.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJ85"/>
    <dbReference type="GeneID" id="9185946"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008881001"/>
    <dbReference type="HOGENOM" id="CLU_2147703_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJ85"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ84578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="112" mass="12207" checksum="C7E0940E08A05AA9" modified="2010-06-15" version="1">MAANVSSAGGGGGEETLTQTQKEILVELRKSLGTLRVMVHLVVVVWWKRRRRLQGRLEGLLVRRFGSAVVLRGRAEAQTKARQWKDCNMVAMGISKGSGGNVESVALYTIVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJ95</accession>
    <name>D5GJ95_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mannose-1-phosphate guanyltransferase</fullName>
            <ecNumber evidence="4">2.7.7.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">GDP-mannose pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">GTP-mannose-1-phosphate guanylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">GSTUM_00008894001</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Involved in cell wall synthesis where it is required for glycosylation. Involved in cell cycle progression through cell-size checkpoint.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>alpha-D-mannose 1-phosphate + GTP + H(+) = GDP-alpha-D-mannose + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15229"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
            <dbReference type="EC" id="2.7.7.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; GDP-alpha-D-mannose from alpha-D-mannose 1-phosphate (GTP route): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.13" evidence="4"/>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840397.1">
        <property type="nucleotide sequence ID" value="XM_002840351.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJ95"/>
    <dbReference type="FunCoup" id="D5GJ95">
        <property type="interactions" value="1215"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJ95"/>
    <dbReference type="GeneID" id="9185956"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008894001"/>
    <dbReference type="eggNOG" id="KOG1322">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJ95"/>
    <dbReference type="OMA" id="GPNCWIC"/>
    <dbReference type="UniPathway" id="UPA00126">
        <property type="reaction ID" value="UER00930"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004475">
        <property type="term" value="F:mannose-1-phosphate guanylyltransferase (GTP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071554">
        <property type="term" value="P:cell wall organization or biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009298">
        <property type="term" value="P:GDP-mannose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06425">
        <property type="entry name" value="M1P_guanylylT_B_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.10.10:FF:000017">
        <property type="entry name" value="Mannose-1-phosphate guanyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000013">
        <property type="entry name" value="mannose-1-phosphate guanyltransferase beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056729">
        <property type="entry name" value="GMPPB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045233">
        <property type="entry name" value="GMPPB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050486">
        <property type="entry name" value="Mannose-1P_guanyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22572">
        <property type="entry name" value="SUGAR-1-PHOSPHATE GUANYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25087">
        <property type="entry name" value="GMPPB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="15">
        <location>
            <begin position="2"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="Mannose-1-phosphate guanyltransferase C-terminal" evidence="16">
        <location>
            <begin position="256"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF25087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAZ84588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="364" mass="39895" checksum="6C36AD1E5BABC6CB" modified="2010-06-15" version="1">MKALILVGGFGTRLRPLTLTLPKPLVEFGNKPMILHQVESLAAAGVTDIVLAVNYRPDVMISVLKKYEEQYGVTITFSIESEPLGTAGPLKLAEDILGKNQEPFFVLNSDVICDYPFKDLAAFHKSHGQEGTIVVTKVEEPSKYGVVVHKPGHYSRIDRFVEKPVEFVGNRINAGIYILNPSVLSRIELRPTSIEQETFPAIVRDGELHSFDLEGFWMDVGQPKDFLTGTCLYLSSLAKKNSKLLTPSSEPFVYGGNVLIDSTATIGKNCRIGPNVTIGPNVVIGDGVRVQRSVLLQGSRIKDHAWVKSTIVGWNSTVGKWARLENVTVLGDDVTISDEVYVNGGCVLPHKTIKVNIDVPAIIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJC8</accession>
    <name>D5GJC8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008941001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430330">
        <property type="protein sequence ID" value="CAZ84621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840430.1">
        <property type="nucleotide sequence ID" value="XM_002840384.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJC8"/>
    <dbReference type="FunCoup" id="D5GJC8">
        <property type="interactions" value="51"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJC8"/>
    <dbReference type="GeneID" id="9185989"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008941001"/>
    <dbReference type="eggNOG" id="KOG0634">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_0_6_1"/>
    <dbReference type="InParanoid" id="D5GJC8"/>
    <dbReference type="OMA" id="FEDNGFQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047536">
        <property type="term" value="F:2-aminoadipate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42858">
        <property type="entry name" value="AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42858:SF1">
        <property type="entry name" value="LD15494P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="1">
        <location>
            <begin position="51"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="445" mass="48304" checksum="02498AD08F0346DF" modified="2010-06-15" version="1">MLANLHKPIDLLRGHPSTRLLAVDLIRESAAKVLSSDLPQDSYAQVRHPLHYGPNMGNEDLRAEIGRWTAERYELKEAIPAERINITPGASYGLMSTLLLCTSPGTGYTKQAFVVSPTYFLAASVFQDAGFSGKLTAIRLNKNTIDTDSLVAHLERISASAPDVQLPSGLEPISRAGAPKRIYKFVMYCVPTYSNPTGETWDLETRRKVVEIARKWDMLVISDDVYDFLGNDGDTSALTPDKKLMPRLVTLDHEIGQKHGMGGDEAGYTVSNCSFSKLLGPGLRCGWIESATGVLAKQMGEGGANRSGGAPSHFASTLIHPLITSGSIDSAISNLTKTYTARCAAIIAAIKTYFPKEATIYGGKGGFFLWIILPENYDAHEITKLAAKDVIVLGGDRSECPGDTLGWGDHCVRVSVSYSEADVAVEGIKKFAEAMKRWENGERSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJI6</accession>
    <name>D5GJI6_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">ER membrane protein complex subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00009032001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the EMC4 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430332">
        <property type="protein sequence ID" value="CAZ84679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840488.1">
        <property type="nucleotide sequence ID" value="XM_002840442.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJI6"/>
    <dbReference type="FunCoup" id="D5GJI6">
        <property type="interactions" value="721"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJI6"/>
    <dbReference type="GeneID" id="9182680"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009032001"/>
    <dbReference type="eggNOG" id="KOG3318">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098404_2_0_1"/>
    <dbReference type="InParanoid" id="D5GJI6"/>
    <dbReference type="OMA" id="QQTFKVI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032977">
        <property type="term" value="F:membrane insertase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015914">
        <property type="term" value="P:phospholipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045050">
        <property type="term" value="P:protein insertion into ER membrane by stop-transfer membrane-anchor sequence"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009445">
        <property type="entry name" value="TMEM85/Emc4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19315">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06417">
        <property type="entry name" value="EMC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017207">
        <property type="entry name" value="UCP017207_TM-p85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="87"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="139"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="28"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR017207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ84679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="187" mass="20612" checksum="3BB12FBE01A5EB7C" modified="2010-06-15" version="1">MAPHGPPRLPPPPSPQWVIDLNNLPSSRPRPNPSIPDPPGFSQGSAGKGKSSKSHPTPTPTETDDLFLKKAWELALAPAKSIPMNAIMMYMSGNTLQIFSIMMTVMLFMNPLKALSTVGSTFARFDNERTHTRLWPVKLAYIGLQIATIALGIWKVNGMGLLPNTRSDWLAWERERQSLELSAVALQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJK0</accession>
    <name>D5GJK0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 14 17">Homoserine dehydrogenase</fullName>
            <shortName evidence="14">HDH</shortName>
            <ecNumber evidence="5 14 17">1.1.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">GSTUM_00009048001</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the conversion of L-aspartate-beta-semialdehyde (L-Asa) to L-homoserine (L-Hse), the third step in the biosynthesis of amino acids that derive from aspartate (the aspartate family of amino acids), including methioinine and threonine, the latter of which is a precursor to isoleucine; production of homoserine leads to a branch-point in the pathway as it can either be O-phosphorylated for processing to threonine, or O-acylated for processing to methionine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-homoserine + NADP(+) = L-aspartate 4-semialdehyde + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15761"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="1.1.1.3"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="12">
            <dbReference type="Rhea" id="RHEA:15763"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:25213"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 17">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 3/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 17">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 14 18">Belongs to the homoserine dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.3" evidence="5 14 17"/>
    <dbReference type="EMBL" id="FN430332">
        <property type="protein sequence ID" value="CAZ84693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840502.1">
        <property type="nucleotide sequence ID" value="XM_002840456.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJK0"/>
    <dbReference type="FunCoup" id="D5GJK0">
        <property type="interactions" value="332"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJK0"/>
    <dbReference type="GeneID" id="9182694"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009048001"/>
    <dbReference type="eggNOG" id="KOG0455">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009116_0_1_1"/>
    <dbReference type="InParanoid" id="D5GJK0"/>
    <dbReference type="OMA" id="IYTRCYS"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00063"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00465"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004412">
        <property type="term" value="F:homoserine dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009090">
        <property type="term" value="P:homoserine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000006">
        <property type="entry name" value="Bifunctional aspartokinase/homoserine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005106">
        <property type="entry name" value="Asp/hSer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011147">
        <property type="entry name" value="Bifunc_Aspkin/hSer_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001342">
        <property type="entry name" value="HDH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019811">
        <property type="entry name" value="HDH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022697">
        <property type="entry name" value="HDH_short"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43070:SF5">
        <property type="entry name" value="HOMOSERINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00742">
        <property type="entry name" value="Homoserine_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03447">
        <property type="entry name" value="NAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036497">
        <property type="entry name" value="HDH_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01042">
        <property type="entry name" value="HOMOSER_DHGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14 17" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11 14 17" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="9 14 16" id="KW-0521">NADP</keyword>
    <keyword evidence="10 14 17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 14 17" id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="domain" description="Aspartate/homoserine dehydrogenase NAD-binding" evidence="20">
        <location>
            <begin position="10"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Homoserine dehydrogenase catalytic" evidence="19">
        <location>
            <begin position="155"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="15">
        <location>
            <position position="229"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="10"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>NADPH</name>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>NADPH</name>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>L-homoserine</name>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR036497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR036497-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR036497-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU004171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF03447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="CAZ84693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="367" mass="38797" checksum="CF5D133CCAE09B2D" modified="2010-06-15" version="1">MSKVYVAVIGAGGVGKSFISQLNAYALRSKKYTISTILISRSSKALLSKDYSGLNLTTWESDISSSSGVKPPSFPDETLKFLTSAPGPMILVDNTSSETIAEEYPRFLSSGVHLVTPNKKGFSSSLALFKAIYAASEAQNGGYVFHEATVGAGLPVLSTLKDLIDTGDEIVKIEGVFSGTMSYLFNSFAPLSPSGAEKSFSACVAEARELGYTEPDPRDDLNGLDVARKLTILARLSGLLVTEPTSFPIQSLIPKPLESAASAEEFLAGLAGFDGEMHKVKTTAESEGAVVRYVGSLDVGKGEVSVGLRNFDRGSTVAGLKGSDNIISFYTRRYGERAMIVQGAGAGADVTAMGVLSDLIKVLERLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJK1</accession>
    <name>D5GJK1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00009049001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430332">
        <property type="protein sequence ID" value="CAZ84694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840503.1">
        <property type="nucleotide sequence ID" value="XM_002840457.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5GJK1">
        <property type="interactions" value="942"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJK1"/>
    <dbReference type="GeneID" id="9182695"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009049001"/>
    <dbReference type="eggNOG" id="KOG1823">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000327_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJK1"/>
    <dbReference type="OMA" id="EGLMAMF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052575">
        <property type="entry name" value="SSU_processome_comp_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057525">
        <property type="entry name" value="UTP20_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046523">
        <property type="entry name" value="UTP20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011430">
        <property type="entry name" value="UTP20_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17695">
        <property type="entry name" value="SMALL SUBUNIT PROCESSOME COMPONENT 20 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17695:SF11">
        <property type="entry name" value="SMALL SUBUNIT PROCESSOME COMPONENT 20 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20416">
        <property type="entry name" value="UTP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23099">
        <property type="entry name" value="UTP20_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07539">
        <property type="entry name" value="UTP20_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="U3 small nucleolar RNA-associated protein 20 N-terminal" evidence="2">
        <location>
            <begin position="869"/>
            <end position="1474"/>
        </location>
    </feature>
    <feature type="domain" description="U3 small nucleolar RNA-associated protein 20" evidence="3">
        <location>
            <begin position="1680"/>
            <end position="1898"/>
        </location>
    </feature>
    <feature type="domain" description="U3 small nucleolar RNA-associated protein 20 C-terminal" evidence="4">
        <location>
            <begin position="2255"/>
            <end position="2574"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2558"/>
            <end position="2587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="2567"/>
            <end position="2587"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ84694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="2587" mass="289867" checksum="482A7D03BE92C421" modified="2010-06-15" version="1">MTRTSMKPPAGKKGGTKSTKNHTFQSFTQKLQNIKIDPVRKVRRRVLDEGFERSFFHSALEKWIDLNLSTTFVELFVRPVLPLSESLPHILHHKETIFGLLVEGIKTRDVNALEPLLDLLTQFAHDLGVEFQGFFKESVGLLAALVAEGFVEAQVVEWVFGALAYLLKYLSRLLAGDLRPLFGVVRGMLGWGGGRGFVRRCAGEAIGFLVRRVRAEVVRDLARAVFEDLRECEGAWVPGYMEGLMGLFKEGCVGVDRGLHSKAPIVLESLIQVAKELGEDIGGRCFKIIEGVLISLIHHTNDETFYPALRVVYAINNPNDELSTRMSARLLYICLTVRKGNRISDWGTAAEVAVANTQIIMGLPDDSSGSGERVKETMWESLKAVAMVMQTADMEAVIARLGRIMDSVREFQDGRLFLPFCELLADTGVERFRVFMLPYFQRFITAKWAIAEDNLLYLIPKLGANGSLVPSEDTDRTTMATVLGDKSPVTAGAMNNIREFLENIGGGGDQDTLRAQAVKIWEYLNAILSLSDSLQGDQLPGGLADALEQLLNGLFSLPENTKLASLTGKVLTMSSKYPSMRSRNLEEPLAVAFPKLADNAVFLQGVVGFFSLSTPKKLTDKALISMADKLITNLSSSSHDIRSHSLRCLELLHVAKSPRKQVSCVISTAQIIENSPLEVSNARNISLYVRRLAGEYSSAAKGSWESRVVPYFCFGLLTVHFSPVWDDASATLSKVAERDEEVVAMLAFEWLERQCETPEASDSGTSDGGKASLNGFECSNLNTIEEVAKKCVMGGFEAVADLERKFAKDLKTPTLSTSIARMQALKVLTEVPRIAEKRSRKLVPMFLEWSTFEDTEDGEDEEETKTQSKWLRKEQSAMLTLFSRFTNPRSLYKSDAVYSALLSLLSSGDPKVQTLVLECIFTWRIPAIRTYEDNLKNLLDDARFRDEITHFVQIGEDESMIQDDHRQKLMPVLLRLLYGRILSRKNATSGKKGMECRRTVVLASLVNFQQWERELFIEIALGNLAEVSGRFVEKRPERYILHMEALGTAHVSNRKQMGFVRMAGDMIRQLGSTLLPFMEKIVDAFLYCLVMATKTIDSREDIIDGLDDAVAVKAARAIRQGGVKCLGALFSSCPTFPWAHYMPAIFDELVNPRLDALPIETSQSPSGLLQLFSTWANSKDTVMFLEDYNQTVLSKIAACLANKSVKDEVVLFIIKMVKQIVSLAGSEGGVGPRLLEPNVDVLLVNLGDILHQSPGKEVLEQAVEAISKIAPFVSGDTETTHLVELSVFLLRQPSRRVSPKAKGDILKILLHFLPLCVVEKESKLYEDTFRCVSSLFGFFKDRENRETLAGVFNVFAERDEELVQVAELSRQLNSFSDRRLDEPDFEQRLRAYGKINEDLYLQFSPKQWIPLIFNMLFFIRDNEELAIRTNSSYALKRFAESCGQKSGTEEKEAYLNILSETLMPALKNGAREQSEFVRMEYVAIVAHVVKECSFWEEVSDMKALLVGDDEEANFFNNILHIQQHRRLRALRRLATTSRRENTQSSNITHFFLPLVEHFVFDQAEDAHNLASETVITIGALAEQLTWTQYRALFRRYTGYLKSKPELEKIVVRLIGTTVNSLARATAAKGNAAAMVKDGGGDVPMGNAMNRLAESLPEQRNLAEDISGGFLSPLSEYLHQKEESTVSLRVPIAISIAKLLKLMPDDMLRLRLPPVLTDVCHILRSRSQESRDMTRKTLTEITTLLGPQYFEFVLKELRGALLRGYQLHVLSFTMHSILVSVTPTYSPGALDYCVGQMVSVIMDDIFGVTGMEKDAEGYISKMREVKSSKSYDSMDLIASVTTLPYLGGLIRPIGALLKENMNLRMSQKIDELLRRITVGLLRNKEVGSEKTLIFCYEVIHEGYRQDTAEAREKEVDPKTQRYLVNLKVPSKAASGVATSSHMYKLTRFALDILRTTLQKHDGLMTSRNLANFIPVIGDALLSKYEEVQISALRVLTTIIRTPLPSIDEGAEVFMGQALSFIKSCPSMNAELAQASLKLMSSVLRERRSVKIKERTIGYLLTRVKSDLEEPDRQGATFNFLKAVLARKVVIPEVYDVLDSVAAIMVTNQTRSVRDLARGVYFQFLMEYPQGRDRLNKQLAFLVKNLDYVHQSGRQSVLEVVNLLCTKVGDSLIQQVLGTFFVPLVMRLINDDSSECREMAGALLRKALERADADNLSTFTMLMKNWLGQDQKPLLVRSALQIYGLYLDAFPTSAKRDLQFVMGELQSIIRASSTDQDAHWENLYFSLQTWARIVLHFPATTFSADQAPAWTSIHDCLSFPHAWVRLSAARLTGLLFAEYAAKATLSTTPLDNGAGLTLSAEAMVHIAHRASSHLNSPELSEELGLQVVKNLLFLTRCFHAAGVGAISSVPMNDPELDDVEEEQHQQEEGRIRRSALEWLVQRISAILRSERNIKKGLLGKRFAMQWIAALIQLLPKGDLEPLAKTLVRPLYNVLEVDGMHLMKDLKPTAQELQSSIAEKLGTTLFAIIYSQVKSEALERRRVRKGKRAILAVTDPEKAAAKKIKKHERKREVRKEKGRAEREGRWGRGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKF4</accession>
    <name>D5GKF4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00009513001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430338">
        <property type="protein sequence ID" value="CAZ84997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840806.1">
        <property type="nucleotide sequence ID" value="XM_002840760.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKF4"/>
    <dbReference type="STRING" id="656061.D5GKF4"/>
    <dbReference type="GeneID" id="9182017"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009513001"/>
    <dbReference type="eggNOG" id="ENOG502S1QC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007655_0_1_1"/>
    <dbReference type="InParanoid" id="D5GKF4"/>
    <dbReference type="OMA" id="ARMFATR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ84997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="820" mass="92717" checksum="A36AE734BE65893F" modified="2010-06-15" version="1">MRNPVLRLLPISRPQLSFSSMICICTPCHHHLGTSSSSFSTPLRGTAIPQKLQVLIHERTSTFDLLKEQADVADASSLGTKLVDEPRLRGNFHLWWSLLGFKRRVYSDTGVKDVWKGLRIRNIDLPVRGSHATLIWQIFLKTGFQDEDFLEEIWEYIKNLDSRNRRWAGAYQFIIGHFLVADGSRARIWHRRLYPRHAPSSWPGFVRSVLKQRVGGHIELREIHAVIERPAGLYGSIIPLLCSMGLFTEAMKWHRHCISFDDLPGNTATADRLIEWTTKYGSLQDMSMLMLSFIQMGVRVKESSLIALIISRPKKFEALGFILARANRVDRSAMGDRFWAVVLGQIQLGTPVLFQCMQTFGEGLNVGRRTVEALKKRVDCSKEKALSLLVEFGMNVQGVEMVPDAEPSVLVDRLVRQIGARGLSGMEELEFLLKEALDTRNWEMFDQALSLPSPSGPTAKVYDLILQSQLRRGTLGDAINLLDHMRKSSISISSASLRLLVKAVLPPRKRAKNPNTMPGFTNDNLIGLIGILTSLLRSGSTNIEPTLWREIFKRLGMKRRLSDLETLALGLVEWYHPARSTITRRRFSTSNFNLPEALTSHPDPQSAPEILPLSPPSPLRLLFPPQFIHAIIQWGFLTFRPHSPNLHKSIPPPPPPEVRQLPRKLSEFAPCVATFTWGIAFARDLKALGVHVDTRSVLRAVRTRLNILYNAPEANGRPVVLSGRRVNRMAVALNHLSLAEMVDITERTWGGSLWRRAGWERGGEGGREWERNMKKEVFSTGIPKRVVERLKRGARVRQMWGVMRLRAGRGRAGRSFVGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKG2</accession>
    <name>D5GKG2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">GSTUM_00009529001</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="FN430338">
        <property type="protein sequence ID" value="CAZ85005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840814.1">
        <property type="nucleotide sequence ID" value="XM_002840768.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5GKG2">
        <property type="interactions" value="506"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GKG2"/>
    <dbReference type="GeneID" id="9182025"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009529001"/>
    <dbReference type="eggNOG" id="KOG1609">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001266_0_0_1"/>
    <dbReference type="InParanoid" id="D5GKG2"/>
    <dbReference type="OMA" id="VGTCYMF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16702">
        <property type="entry name" value="RING_CH-C4HC3_MARCH6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000287">
        <property type="entry name" value="RING finger membrane protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056521">
        <property type="entry name" value="MARCHF6-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13145:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MARCHF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13145">
        <property type="entry name" value="SSM4 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23113">
        <property type="entry name" value="MARCHF6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="115"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="266"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="808"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="859"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1043"/>
            <end position="1061"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1093"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1140"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1199"/>
            <end position="1222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1242"/>
            <end position="1260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1401"/>
            <end position="1424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1444"/>
            <end position="1462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1498"/>
            <end position="1523"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1535"/>
            <end position="1552"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1589"/>
            <end position="1610"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1630"/>
            <end position="1648"/>
        </location>
    </feature>
    <feature type="domain" description="RING-CH-type" evidence="17">
        <location>
            <begin position="26"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="34"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="394"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="632"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="746"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1674"/>
            <end position="1699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="16"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="430"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="556"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="572"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="634"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="753"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1687"/>
            <end position="1699"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAZ85005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1699" mass="186823" checksum="66D8FFB6073DF2D6" modified="2010-06-15" version="1">MASGSGPQVDIMNDPAYNSPSASGSNDNGEPDHCRICRSEGSREEPLFHPCKCSGSIKFVHQDCLLEWLQHSQKKHCELCKTPFHFTKLYHPHMPTTIPTPLFLDKLLRHLLRNLLLWARALLVGCVWLGGLPYCVRWMWRMWFWLSDGGWWADQAYNRARTALPTTTPLTNSHGTPPASSAVMNLTGSAFGSGIIGFLRSPLLSGLVMSPVNGGKVTANATTAINATASNATISIGARLGGLFIDPSPFHNFTRYPAVNGVIVDIFEGQLLTALIVISFILIFLIREWVVQQQPGLGGAVALQQLDELQAPQVENIQLPEMNPPPQDRIAGEHEIAPEQFRGGALPGRAGREPPRAIARPRRPRHFPEAAPVANAGVDPVRDRPAQKNYGQGYLLGGGSSEPATPRLGDLGESSRRPRVNRKAMSQPTEIRRAMEERTEAPDGSSRGLGTLATCKIEFPSVGRDKTPSPVYTPESGNSSGSRTPARFGRTPSPVGFNRSNIDVGAPMGRGGPLHNFSGNKSPKIAAVRESPGSAPVEGQIDKGKRPESPFYENSTDNEDQWEDEEEEDDMVDPKGKGKEIERPSAHVDLSSYYGSPATGSFDFAMAGRHNFTFEPRSDLIKVRVPTPDAIQYSPPSISLPSTPTTGGSLPDHSSLTSRAGDESARIGLWEGISQDYDEAEGMSNPDAQNAGHEVGEHITAGSLEAQGLEVDAIRAPVPAQAPIPRQPHQPFVWNRDEVVAREVAQGRAEGDTGTEDGADDGTDAEDNEVADGPGIELPPDAQQAIADEDAADDFDGIMELIGMRGPLAGLLQNAAISVVLITATVALGVAVPYVMGKIVMIILANPIFFLIKVPTTVVSFVAEFAVEFSTCLVFSLLLVVEQIMRFLFRPIERVVPVFSGVSSCLFLSDILRRLALDGQGRVTQKMANLKSIYIDLRTFPSTGAPPVSALTRQGWLRASLGVSWAIEKLGFGTNRTYPLSFLQDSGANNSTPVALFSSGFSKVIAALFQTPQNTTNTTAQDTSLDSLPPLHPVYIWSVWDRVTAVFIGYALFTIVGMLYLQNARKRGLALNGRVVEKIAVELLLQAGGVMKVVLIIGIEMLVFPLYCGLLLDFALLPLFEEATILSRIQFFADFPLTSVFVHWFVGTCYMFHFALFVSMCRKIMRSGVLYFIRDPDDPNFHPVKDVLDRPVMMQLRKIAFSALIYGVLVIVCLGGVVWSLYLVTESALPIHWSSNEPVLEFPVDLLFYNFFVPFAVQFVKPSDGLQKMYGWWFRRCARFLRLTSFMFGDRTPDEEGHHVRQGWAARLLRKNGDVNHPIIEADALKETRGAGVEVYFQRDGRYVRAPASDSVRRPKGRRVFIPVTEDNVRLDEEDPVDLANGLNPSDFKLVYLPPWFKTRIGLLVLAIWGFAAFTGIGVTIGPLLLGRTVLKQLVPPNIKLNDIYAFSVGIYIIGAFVYAGAKWKLARSFIRRGMRAARESESLWWAMSKWLVRVAKISYVLTAFGVVLPTLLALMIEFYVIIPIHTYFSNDDQHVIHFIQDWTLGVLYIKMMGRMLLVDRDSAWARSLRDVVARGYLDPDVKLATKHFIAPAGGFMLVALLLPLGLGSIAANTFLRNAPARKISQIYRYSYPAVFAIFFLVGLCYLWKILVDSWKQSIIDEVYLTGEQLHNHGEDSPPTAAEVPATVSSATTPAGAQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKM0</accession>
    <name>D5GKM0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Tubulin alpha chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">GSTUM_00009636001</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13 15">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4 15">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430340">
        <property type="protein sequence ID" value="CAZ85063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840872.1">
        <property type="nucleotide sequence ID" value="XM_002840826.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKM0"/>
    <dbReference type="STRING" id="656061.D5GKM0"/>
    <dbReference type="GeneID" id="9184725"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009636001"/>
    <dbReference type="eggNOG" id="KOG1376">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015718_0_0_1"/>
    <dbReference type="InParanoid" id="D5GKM0"/>
    <dbReference type="OMA" id="RRVTDNC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02186">
        <property type="entry name" value="alpha_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000005">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000006">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000011">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002452">
        <property type="entry name" value="Alpha_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01162">
        <property type="entry name" value="ALPHATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11 15" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 15" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="16">
        <location>
            <begin position="13"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="17">
        <location>
            <begin position="212"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAZ85063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="411" mass="45554" checksum="255B0BA6D4CB9973" modified="2010-06-15" version="1">MAAAADVAADGSFDTFFSETASGKYVPRSIFVDLDPSPVDEIRTGTYRQLFHPELLISGKEDAASNYARGHYTIGKELLENVLDRVRRVADNCSSLQGFLVFHSFGGGTGSGFGALLLESLSADFGKKSKLEFAVYPAPRVSTAVVEPYNAVLSTHSTIENSDCTFLVDNEAVYDICRRNLDIPRPSYEHLNRLVAQVVSSITSSLRFDGALNVDLGEFQTNLVPYPRIHYPLISYAPVISASKSTHESFKVADLTFQCFEPNNQMVVCDPRNGKYMAVALLYRGDVVPRDCTSAIATIKAKATFNPVDWCPTGFKIGINYQKPMRVPDSELAPIDRSVSMLSNTTAIAEAWSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFFEAREDLAALEKDYEEVATDTVPEAELDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKM4</accession>
    <name>D5GKM4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RNA polymerase II transcription factor B subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00009642001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the general transcription and DNA repair factor IIH (TFIIH) core complex which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to TFIIK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module TFIIK controls the initiation of transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the TFB2 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430340">
        <property type="protein sequence ID" value="CAZ85067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840876.1">
        <property type="nucleotide sequence ID" value="XM_002840830.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKM4"/>
    <dbReference type="FunCoup" id="D5GKM4">
        <property type="interactions" value="448"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GKM4"/>
    <dbReference type="GeneID" id="9184729"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009642001"/>
    <dbReference type="eggNOG" id="KOG3471">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027280_4_0_1"/>
    <dbReference type="InParanoid" id="D5GKM4"/>
    <dbReference type="OMA" id="KGFIIIE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000112">
        <property type="term" value="C:nucleotide-excision repair factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2610:FF:000001">
        <property type="entry name" value="General transcription factor IIH subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040662">
        <property type="entry name" value="Tfb2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004598">
        <property type="entry name" value="TFIIH_p52/Tfb2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00625">
        <property type="entry name" value="tfb2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13152:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIH SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13152">
        <property type="entry name" value="TFIIH, POLYPEPTIDE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03849">
        <property type="entry name" value="Tfb2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18307">
        <property type="entry name" value="Tfb2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription factor Tfb2 C-terminal" evidence="10">
        <location>
            <begin position="382"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF18307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ85067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="460" mass="52241" checksum="BF8BB4D83EC2D014" modified="2010-06-15" version="1">MSGNIRGFGYLESLPPVTFRRLYQQPATALAIFRRMLPNLAKTIVTTLLYNETPVTLGDLEALVRPESYREREDAFSKLRRLHIITENNLDVSLDPVFKKNFRLAGDHHSFGVPCNAEDKKKITVQFLDDYATRQFEAILHYMVGTHNEVKPSKGVITLLTKGGLMERYGAGSSATITKNGFSFLLQDGNPQIWALLIQYLEMSEELGMEQTDVLHFLLMLGSLELGQAYSVNTLTETQKLMLADLRDYGIVYQRKSSSDRFYPTRLATTLTSESGGLRSASASMSSAMAKDAEEGKGFIILETNYRVYAYTDSPLQIAVLNLFVKLSTRYPNLVSGRISRRSIQEAIKMGITADQVIDYLSAHAHPQMRKSLVTLPPTVVDQIRLWQIEGERMRTTTGFLFKDFHSTQEFEEVAKYAEELGVLKWKKPARRCMFVTRHEQIADWLKRRAQQQQPKKVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKN2</accession>
    <name>D5GKN2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00009667001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430340">
        <property type="protein sequence ID" value="CAZ85075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840884.1">
        <property type="nucleotide sequence ID" value="XM_002840838.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKN2"/>
    <dbReference type="GeneID" id="9184737"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009667001"/>
    <dbReference type="eggNOG" id="ENOG502QRRW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044368_2_0_1"/>
    <dbReference type="InParanoid" id="D5GKN2"/>
    <dbReference type="OMA" id="MPRTIAN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47660:SF3">
        <property type="entry name" value="FINGER DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G03310)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47660">
        <property type="entry name" value="TRANSCRIPTION FACTOR WITH C2H2 AND ZN(2)-CYS(6) DNA BINDING DOMAIN (EUROFUNG)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="50"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="8">
        <location>
            <begin position="86"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="148"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="34"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ85075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="551" mass="61591" checksum="B090234A8FE30C11" modified="2010-06-15" version="1">MAQSWAAQSSSSSQSNNSAAAPSPHTAAAHRPKSTNTDNVQSAPSPSVLFKCSVCDKGFEQRSSRDRHQRRCRQKQISGTIPRKKSCALCTQAKTRCDLLTPSCSRCRNKGLDCHYYHQPPPQHSHSHHYATATTPLRTTELVHRRSPVRLSEEVSLPTPACTDNGLPDEFSDGSLRHTGNHRSGSSNNGIGDVEITDWGDINHNFLEWNIFAGSTGDMELHNVCPIGSESPVSPQGAYQHLYNPNHLGNPMDQQMRLAPRQLSIESFKQMRKILVENAPAPGPKNKFIFIDRVCRGYPKMMTGRNGPPPFIHSSHLIPGRMTSALANCRGLVDMYKAMTPDNKSFVMKSIATEHGRILAEAEHVRVNEVEKIGVLQAALIYGIIRHFENDPTFDRAAMVSMETMAVNVGVASLIAKGELEGVRPVWEDWIIAESKRRTMITLYMLDGSFHYAHNIPTFTCGELNEILLPTPTILWQSRERSKWEYEYENYLSDLRGRRLPKMADILSTEPKYLNIEEWIGGMDNFGVTVLAMVENCREDVPDAVEDGGRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>D5GL06</accession>
    <name>D5GL06_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00009863001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430342">
        <property type="protein sequence ID" value="CAZ85199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841008.1">
        <property type="nucleotide sequence ID" value="XM_002840962.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GL06"/>
    <dbReference type="GeneID" id="9185793"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009863001"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_551181_0_0_1"/>
    <dbReference type="InParanoid" id="D5GL06"/>
    <dbReference type="OMA" id="IASPPEC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051061">
        <property type="entry name" value="Zinc_finger_trans_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46179:SF19">
        <property type="entry name" value="C2H2 FINGER DOMAIN TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46179">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="373"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="404"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="438"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="245"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="292"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="464"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="214"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="300"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="322"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="464"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ85199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="495" mass="53065" checksum="573FFD3B1584B2FF" modified="2010-06-15" version="1">MDIDTTTADAPKGHDVPEPEKIASPPECEGGPVEIELSSSETAVSLMALAAGDDRQPFTSAPSLPGVPSKAGGTAWAPNARAKSPNPSQSRGPPPSPSPSTQLFQDNKLPPPNSPTQLPSMISTMNSQPASPPNSLPPGNDGRHTTLPPVSSIQVLAEMAEKAMPTQNSNLWTNNRMPSTPRTVYPTVSSHVNVAPSNRPTQAPQSPGLPAPPGQSQHGGFSSDANPRPPVHHLTLQQRQFYLNQDPPLSPYYTNSHQHYQSPYPPMKEGASPNTNGSTVPYQSSITHPRELPGILQNHSSPSSAPAFFTPPALPGRKDSTTMEYYSADNTPPSGPSTGDTMPSSGTDDTITSPRSRSTVAPSAQGPLASGGFKCEFAGCKASPFQTQYLLNSHANVHSSARPHYCPVKGCSRGEGGKGFKRKNEMIRHGLVHDSPGYVCPFCPDREHKYPRPDNLQRHVRVHHMDKDKDDPLLRDVLAQRPEGGNRGRRRRLGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>D5GL34</accession>
    <name>D5GL34_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00009948001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430344">
        <property type="protein sequence ID" value="CAZ85227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841036.1">
        <property type="nucleotide sequence ID" value="XM_002840990.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GL34"/>
    <dbReference type="STRING" id="656061.D5GL34"/>
    <dbReference type="GeneID" id="9185821"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009948001"/>
    <dbReference type="eggNOG" id="KOG1177">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_738083_0_0_1"/>
    <dbReference type="InParanoid" id="D5GL34"/>
    <dbReference type="OMA" id="YMFILES"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031956">
        <property type="term" value="F:medium-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43201">
        <property type="entry name" value="ACYL-COA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43201:SF30">
        <property type="entry name" value="AMP-DEPENDENT SYNTHETASE_LIGASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="1">
        <location>
            <begin position="64"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="375" mass="39916" checksum="5B534E8005F42F35" modified="2010-06-15" version="1">MLGCRLRGLGFSGIGIVAAEVTTEDLQRYGTTAVTRLDHRLTPSGNNPRRSFPPSLLIASKANVTSVISSHQNTRLTYSSLHTQSTSLAHGLKSLGVQRGDRVAVSLGNNIEFALATYAIFKLGAVLVPLNPSFNTAQVVSALNHLSATHLLIGLETNLPRKPPRSNYKLLRELVPGCQSGGVVASELVPSLGRVVVVDNTRDKGKGGDGFKAFSKFPDVLEDGEGKAHLTEEGMGVDDVVNIQFTSGTTSLPKAACLTHRSILNNGKSIGDRMLLTNKDVICCPPPLFHCFGSVLGYMASATHASCIVFPAECFDAKATLHAVQQEKCTALYGVPTMFTAELELLASGEVEQKGFEHIRTGIAAGIYDSPSLND</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLI8</accession>
    <name>D5GLI8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Nitrate/nitrite transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00010227001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430349">
        <property type="protein sequence ID" value="CAZ85381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841190.1">
        <property type="nucleotide sequence ID" value="XM_002841144.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLI8"/>
    <dbReference type="STRING" id="656061.D5GLI8"/>
    <dbReference type="GeneID" id="9186125"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010227001"/>
    <dbReference type="eggNOG" id="ENOG502QPIC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024204_1_1_1"/>
    <dbReference type="InParanoid" id="D5GLI8"/>
    <dbReference type="OMA" id="TFWAWNL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015112">
        <property type="term" value="F:nitrate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015113">
        <property type="term" value="F:nitrite transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042128">
        <property type="term" value="P:nitrate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000382">
        <property type="entry name" value="Nitrate transporter CrnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044772">
        <property type="entry name" value="NO3_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004737">
        <property type="entry name" value="NO3_transporter_NarK/NarU-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00886">
        <property type="entry name" value="2A0108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23515">
        <property type="entry name" value="HIGH-AFFINITY NITRATE TRANSPORTER 2.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0534">Nitrate assimilation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="35"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="72"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="96"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="198"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="350"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="384"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="409"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="439"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="474"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="34"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="246"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ85381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="502" mass="53898" checksum="A93E4144049A9682" modified="2010-06-15" version="1">MQLSLLWKAPEVNPANHKARSVPILNPYDKYGRTFFFSTFGFMIAFLSWYAFPPLMGKTIKKDLALTQAEVANSNVLALTATLVVRLFIGPLCDRFGPRLCFAGILLAGSIPTALAGLVHNATGLLALRFFVGVLGATFVPCQVWSTGFFDKNVVGTANGLMAGIGNAGGGITYFVMPAIFDSLVHDRGLTPHKAWRVAFVVPFILIVAMALGMIFICEDTPTGSWADRHNFTPRTIEGKIVDATGNINDAPTADESPKGAPYSEKDKKTSDPENASSSASIDLETIVQSEVIQAPTLQEAIQVIFSLQCLMLAAPYACSFGGELAINSILGSYYIKNFKYLGQTNSGRWAAMFGLLNVFFRPAGGIIADIIYRHTKSVAAKKWWLVFIGCMQGVFCLAIGILNPHDESTMFGLVAGLAFFMDASNGGNFALVPHVHPFANGILSGVVGAAGNLGGVIFSVIFRYNGTNYDKVIMIIGAICIGINLGISWIRPIPKGQIGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLJ1</accession>
    <name>D5GLJ1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00010232001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC29A/ENT transporter (TC 2.A.57) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430349">
        <property type="protein sequence ID" value="CAZ85384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841193.1">
        <property type="nucleotide sequence ID" value="XM_002841147.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLJ1"/>
    <dbReference type="FunCoup" id="D5GLJ1">
        <property type="interactions" value="243"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GLJ1"/>
    <dbReference type="GeneID" id="9186128"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010232001"/>
    <dbReference type="eggNOG" id="KOG1479">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021611_3_0_1"/>
    <dbReference type="InParanoid" id="D5GLJ1"/>
    <dbReference type="OMA" id="GSPWTTK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034257">
        <property type="term" value="F:nicotinamide riboside transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015205">
        <property type="term" value="F:nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002259">
        <property type="entry name" value="Eqnu_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10332">
        <property type="entry name" value="EQUILIBRATIVE NUCLEOSIDE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10332:SF88">
        <property type="entry name" value="EQUILIBRATIVE NUCLEOSIDE TRANSPORTER 1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01733">
        <property type="entry name" value="Nucleoside_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016379">
        <property type="entry name" value="ENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01130">
        <property type="entry name" value="DERENTRNSPRT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="87"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="124"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="158"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="184"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="223"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="260"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="321"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="360"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="423"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="462"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="12"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ85384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="484" mass="53727" checksum="31A77F770BA0F2A9" modified="2010-06-15" version="1">MTPNSRTSITLQPPPRSPSPPSPYHSPSTPSTFSALEVVVPDSHSSTSYTMQRLREWWKPTYEPLEDQDEREDMSSSKAQRVSRFEYFIFLSLGVAMLWSWNMFMACATYFQRRFAENEFLLNNFQSLILAVSTITNLGSAVYLSYRQKSASYPWRICASLVINCGVSTVLALSAVVFRVGPEAYITILLTCVFWASWSAGLSQNGIFAFVNKFDGIYTQAIMTGQGVAGVLPAIAQIISVLAIPQSPGTEGSTASPKSAFIYFLTATFVSGSCLLLFLLLLSRHRISPHKSGSEVIDSEDLTPETHTQVSLWVLLKKLKYLSFAVWLCFLVTMVFPVYTQVILSVRPEDSSPRMFKPDVFIPIGFMLWNLGDLSGRVVCGWRRFACDRPKLLALISIARLVFIPLYTMCNIKGHGAVISSDLFYWLVQFTFGMSNGWVGSNVMMSTPGWVDDDEKEASGGFMGMCLVAGLATGSLASFLIFNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLQ1</accession>
    <name>D5GLQ1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00010311001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430350">
        <property type="protein sequence ID" value="CAZ85444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841253.1">
        <property type="nucleotide sequence ID" value="XM_002841207.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLQ1"/>
    <dbReference type="STRING" id="656061.D5GLQ1"/>
    <dbReference type="GeneID" id="9182747"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010311001"/>
    <dbReference type="eggNOG" id="KOG4441">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_292076_0_0_1"/>
    <dbReference type="InParanoid" id="D5GLQ1"/>
    <dbReference type="OMA" id="MDERISD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18186">
        <property type="entry name" value="BTB_POZ_ZBTB_KLHL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24413">
        <property type="entry name" value="SPECKLE-TYPE POZ PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="1">
        <location>
            <begin position="894"/>
            <end position="960"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1044" mass="118141" checksum="C9E5061704E42249" modified="2010-06-15" version="1">MALQSDRKTPISEEKNGPLEGLKWNNWIFKEGIVPLYLLFLRHLMKLHGTGGYRFWPAPPSELKNPDHASLTISTEFWKKVGKSLYDLYPQAPPPLLTPRKSQSQATENLSIKRVIFDFLSPGQSKFIVPLLMQLGITNIVTLPPTIMKGLTGATTDEVSLLSVTPAYVHDILRNPTRCKLLQDHWKKDPGNLARNTSMLLSFLLQDTSEDCLTGCSLLPLDGGSWGTFSRKSTSQIQYFTSRTPLERSILEITDGRLVSKDLECNVINGLLEKMNISLLSFDHIPSLCRLVESKDPEYRKTWLANVWEYFTFCVEKDPAYMDYYLKSIESLPVYCGSVVGKPGSLRFLTPLSFASGSPPAIIDPNIPPSEKGKSALFQALNGLILVNRSTFPKTGLIAESLSWPQGVRRLIKSIGSLPSTIHTAHSLSQIFSGMPAEGVEALSNLILPHISLLLNEDYSLVGTLKQLPIWPVLSSSFQSAEKLKLAPHTSLTLTRMIDQTTFLRPDLASKHRDELQKLGVPQLSYLDFLNNEVGLARGYLPDKKIGEYRKFTEIVYRVKPGIFQSYNLAVNGDLCFCLPITLYDSSVHLFQAAFRDQEGSRFLHKELVGSAIWKAFLIQNISGPTYIECARSIERRNNRTLPDDQLESDSCTVFNHLCWDYQEMHSWPVSAWESLSNIRFAPAQEAITSSGHSQLRSNQGKIFRQRNRLIALSEAVDPKFEGISWSQKPVLPVRMGPLALGKITSNKPMITPTTVLGHLEFLASHREIITKAELPPRISEIKKAYEYLEEKVPAYAIPESVPIWLNVENEDLVGMTPETFRNSWSCTRDLCLNSNYDSGEIKRVRSFLGRFHQLLVHARVRRIIPPPSIPEPGKTQSPILEGLLNLREQKALFDVTITAPPQTFKAHKVVLASVSEYWKGMFTSNCRESSTAEISLQDDPSTVKVLLDYIYTNKFIKPPREDDVTRQLENLLDQLEKSEKWLLPSFQHSMENYLSDPHWIRPETVKPILRSAKMYKADRLADVCRKYIENNRVIVERESPNEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLR4</accession>
    <name>D5GLR4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00010369001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430351">
        <property type="protein sequence ID" value="CAZ85481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841290.1">
        <property type="nucleotide sequence ID" value="XM_002841244.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLR4"/>
    <dbReference type="STRING" id="656061.D5GLR4"/>
    <dbReference type="GeneID" id="9186465"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010369001"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_891953_0_0_1"/>
    <dbReference type="InParanoid" id="D5GLR4"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000634">
        <property type="entry name" value="Zinc finger X-chromosomal protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF60">
        <property type="entry name" value="STRIPE, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="246"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="276"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="18"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="136"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="204"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="18"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="111"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="121"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="146"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ85481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="312" mass="34702" checksum="4AE55BA9719B2506" modified="2010-06-15" version="1">MATLYLGLEENLLSSSPNLRSYSISSTSSGASCSTTQSHPSCYRTLAPKADNTSPMSFPSRPFEDGFLGLRERKTHNGQSIAPNLLSIKVEGAHQTSNPPLDPAFHPSQQMEDMLQQQQQNFSTPNYDQSYPRITNYPEYGPQRYSSNSLSPSSSNGDCLYTTHNRMFDCSQSFAPWGHESNSREACAAMTALELLMPKVGIGSEGEEDVDMSDRDSAGSSPGPTVARRKRRAPHPLVISSRDKPHVCSVGDCESRFKRPEHLRRHEKTHTGERPFSCTICGRRFGRNDNLQTHKKTHMKMTGRNEYIPGLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLX0</accession>
    <name>D5GLX0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00010452001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PA-phosphatase related phosphoesterase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430351">
        <property type="protein sequence ID" value="CAZ85537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841346.1">
        <property type="nucleotide sequence ID" value="XM_002841300.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLX0"/>
    <dbReference type="STRING" id="656061.D5GLX0"/>
    <dbReference type="GeneID" id="9187317"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010452001"/>
    <dbReference type="eggNOG" id="KOG3030">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021458_9_1_1"/>
    <dbReference type="InParanoid" id="D5GLX0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008195">
        <property type="term" value="F:phosphatidate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046839">
        <property type="term" value="P:phospholipid dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03390">
        <property type="entry name" value="PAP2_containing_1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043216">
        <property type="entry name" value="PAP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165">
        <property type="entry name" value="LIPID PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165:SF158">
        <property type="entry name" value="PAP2 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_4G08970)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="66"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="197"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="227"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="250"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="8">
        <location>
            <begin position="127"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="335"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ85537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="488" mass="53494" checksum="9DDCA9808E4CB483" modified="2010-06-15" version="1">MPILDIFTKRKLPNRVVLSYIFDYVVIIVFILIFSALDLAEPYHQHFSLNNESLQYPFAEKERIPAVWAGVLSCLFPLVFIIFWTMLIDGLYSHHKPPNSRRRLLGNNGAWTLSDRLWEMNCGILGLGLSVAAAIVMTGALKNTTGKPRPDMLARCKPKEGAQNAPVYGLASYDICTGDKHILKDGFKSWPSGHSSIAFAGLGYLSLFLAGKLHVFDTRGEVWKTMLVLIPLLAASLVAVSRIMDARHHPFDVITGGMMGFFVAYVSYRQYFPPLSETWKKGRAYPMRSWGTDTAERKQMQLGMYENNPSRGGLVADEEEGGKIGVYNPSAAPMTRGPSAGAPGIVGSGNGSGSRFSDESAAIQARRREAQRIGTMRQPSDLDDYDEYSPQGEHSGGEEPETYELRPGPLKPFRAPGIARTDSQESADTRWGVSRATTAITGSGAGPPPVPGGFEPVKRTDTGFESTDEEKSPPATKDTARRVQLIDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLY4</accession>
    <name>D5GLY4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00010474001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430351">
        <property type="protein sequence ID" value="CAZ85551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841360.1">
        <property type="nucleotide sequence ID" value="XM_002841314.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLY4"/>
    <dbReference type="STRING" id="656061.D5GLY4"/>
    <dbReference type="GeneID" id="9187331"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010474001"/>
    <dbReference type="eggNOG" id="ENOG502RXM6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009483_0_0_1"/>
    <dbReference type="InParanoid" id="D5GLY4"/>
    <dbReference type="OMA" id="WHPLGVH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019321">
        <property type="entry name" value="Nucleoporin_Nup88"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037700">
        <property type="entry name" value="NUP88/NUP82"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13257:SF0">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13257">
        <property type="entry name" value="NUCLEOPORIN NUP84-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10168">
        <property type="entry name" value="Nup88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="6" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="750"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="756"/>
            <end position="765"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ85551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="858" mass="95336" checksum="6C58977098B171AB" modified="2010-06-15" version="1">MATVDDDRLPWLSRPSTGYDLFTKTPENPTSYPSLVTTEDEGTAVGPMRVMANRGTEIFVGRGNEVRCADLQDLKARHTPRAGIRGGGHREYKMLDLPELDFEIHQLEISRDGLLLAIIGEKELAVCVLPAEGLARMDGSKLRVPAFNMVGPNYHNSEKGSRIVRAIWHPLGVEGASLVVLTSESIVRTYDFTIGQDFTFEIPDQTFDLHALGGRARHVGGFFADGEEMEAASCCFGDGYQGWRPFTLYILMRGGDVYALTPVVPSRWKAPREYLQSLSLDISWGLENLEGNVTTEEKLVLRQQTKWINHVLNQEANIGAVYIGSTVSPRKKALTCLVRPGIVGPIPQLQGPFLFQPAPPELSSDEYGCDIFHIEHHPVGVIGILFSNGKVDICMEPESLKAKWVDKRIRRKGAEDESDQSTQGLPVISGYETIDLGFPPGSSCSGISWPVFSRDPQSNQVWYINHNLGVAAISMKPWLSKLATVLDGDEDDGFVAKSMEKNPQSTVYKPIQNANKPQSLARPMIGSVAVYEAYLGYLLIAATKTNSQSVEFEDVQGRASGVDPRASSITTTRSLVHTSQLSLTQSPAHCGHQHRLSLLTSHFTSPPELTIPSALHNLLKPTNPQHKKLLASPIHFTTETNDLLRAARDTLKMEYDRIMEIAQEMYDRATAQRIEFRKQLESIHKASEILTRIQNRDVRARLDRFLTRQEELQARADGVLRILIVKNELGIGDAEKKWFREVAKVEEKIGGGAEKEQEEEEEREGAEEAEKDSNTLAGRREIVARLAGELCPLVGEDGASPGEGRRGMKTVVGRGEDVPEEVRMGKLRILRELLEREDALVKATKKKLENLTLEAERV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>D5GM80</accession>
    <name>D5GM80_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00010565001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430352">
        <property type="protein sequence ID" value="CAZ85623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841432.1">
        <property type="nucleotide sequence ID" value="XM_002841386.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GM80"/>
    <dbReference type="STRING" id="656061.D5GM80"/>
    <dbReference type="GeneID" id="9187407"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010565001"/>
    <dbReference type="eggNOG" id="ENOG502SNJU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_344899_0_0_1"/>
    <dbReference type="InParanoid" id="D5GM80"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057218">
        <property type="entry name" value="DUF7896"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42031">
        <property type="entry name" value="KEY LIME PATHOGENICITY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42031:SF1">
        <property type="entry name" value="KEY LIME PATHOGENICITY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25438">
        <property type="entry name" value="DUF7896"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="516"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="70"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="216"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="382"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="625"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="78"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="221"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="256"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="284"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="414"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="448"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="461"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="629"/>
            <end position="655"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ85623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="820" mass="87788" checksum="EBDAAB2A495F6C17" modified="2010-06-15" version="1">MAAPPLGIPRREGAPLDLKSYVLGFTKFREENPNLSPGECADVFCGAATGEHGAVPVQDESAGSDLRAVTQKAGSCPGGSQSLTSPIESQRMSHSNSTSSYFGTAPTTQQTFAFMSGRSPHQYQVSNSVTCRPGYHETAPPPKASQHMSFDDIKSMVLQMGGGGDLALTDYLSQNDSSEPFPLARRSVSGAAQTFCDDSIDKDYSFSIDGSPGICAPPMQPQLSNPTSRCSTGGDESYLESPGLLSHPMTRHSRSHSNASHMSRSSHFSSQGESGSPTDRFPYMQNSGTPMSRQASNASLTHGAMMLRLSSSTGNSTSGNGGGDYDAFFGRGDMDTMLNGVGLFASDAELSSSLGKATGQHMLKRASSTSVEDLDHSLAIAVEHEMRRDPSTASESVKSRKPKRVKEGAEPTAAVQNEGVSTTIATGPGVPEKEKQGRQAQRKGGSGSSANRQGSTTTDKATQRESNDKMETTGSLPTGGRVLAASKPGKSGPKYTAVSTTKPCYVRPPHPRVHCKFCNECPDGFRGDHELRRHVEREHAPTRKMFVIKDISEDGQFLAKCKACQSGKRYGVDYNAAAHLRRQHFTKKDEKKKKLPFDSSAPEMADLRKWIEEVQVVVDDDVEYSPELPRGKDVSKADNPRERVDKESTSAREGLKEEDISRVSCSRKTAAGNCTAPVVVQDGVLDVSDIPPEQTTFGSIPWERPDNWYEPCGVFDLSHNYGVQFTVSGGSIPLADALNRQDNAAGEQSGMTLPQHQILAHLLQAGRAVQPPTGGETASTIFANLPGPAATKGTSGVFCDDDLAMAIPGPEPIFEVEDFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMB8</accession>
    <name>D5GMB8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00010640001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430354">
        <property type="protein sequence ID" value="CAZ85661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841470.1">
        <property type="nucleotide sequence ID" value="XM_002841424.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMB8"/>
    <dbReference type="FunCoup" id="D5GMB8">
        <property type="interactions" value="948"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GMB8"/>
    <dbReference type="GeneID" id="9184384"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010640001"/>
    <dbReference type="eggNOG" id="KOG2484">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011106_5_5_1"/>
    <dbReference type="InParanoid" id="D5GMB8"/>
    <dbReference type="OMA" id="FKLDGLW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030684">
        <property type="term" value="C:preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1580.10:FF:000006">
        <property type="entry name" value="Nuclear GTP-binding protein NUG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000844">
        <property type="entry name" value="Nuclear GTP-binding protein NUG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030378">
        <property type="entry name" value="G_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014813">
        <property type="entry name" value="Gnl3_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023179">
        <property type="entry name" value="GTP-bd_ortho_bundle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050755">
        <property type="entry name" value="TRAFAC_YlqF/YawG_RiboMat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11089">
        <property type="entry name" value="GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11089:SF30">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN-LIKE 3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08701">
        <property type="entry name" value="GN3L_Grn1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51721">
        <property type="entry name" value="G_CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="CP-type G" evidence="9">
        <location>
            <begin position="185"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="147"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="21"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="57"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ85661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="536" mass="59091" checksum="2F3CEA643157D09D" modified="2010-06-15" version="1">MVKPRKKSKRVPVRLRHKIEKRSNEKARKERKEAKKHPELHRKKKNDKLNIPNSFPYKDRLLSEIEEGRRLKEEEQLRRRELAKQRKKELADGAEGKEDAMQILEEGDEGSSELYSDAMDEDDADAGTGGSAMAALLASARARAAEYEAEHGDLSGESDDDSDSETGIPDIESKSTRKDTSRKAFDKMFKQVVDAADVILYVLDARDPESTRSRDVERQMMAADGGEKRLILILNKIDLVPATVLKGWLDYLCRYFPTLPLRASGSASNAQTFDHKSLTAQGTSATLLKALKSFAHAKQLKRSVSVGVIGYPNVGKSSVINALTGRLGGSQTACPTGAEAGVTTSLREVKLDKKLKLLDSPGIVFPGNSSQGGSKDDQARLILLNAVPPKQILDPVPAVALLIRRLSTSQTLFNKLLDLYNLPPLVGTTTTDVTTDFLIQVARKRGRLGKGGIPNIEAAAKAVIMDLRDGRIQGWVDPPTALHNTLNQGVKPTTVAITTDDQKEIVKEWAKEFKLDGLWDEDCNEEGQSEEMEMQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMT0</accession>
    <name>D5GMT0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DASH complex subunit DAD1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Outer kinetochore protein DAD1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">GSTUM_00010918001</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
            <location evidence="3">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DASH complex DAD1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430359">
        <property type="protein sequence ID" value="CAZ85823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841632.1">
        <property type="nucleotide sequence ID" value="XM_002841586.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMT0"/>
    <dbReference type="GeneID" id="9185159"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010918001"/>
    <dbReference type="HOGENOM" id="CLU_3015906_0_0_1"/>
    <dbReference type="InParanoid" id="D5GMT0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042729">
        <property type="term" value="C:DASH complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051010">
        <property type="term" value="F:microtubule plus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013958">
        <property type="entry name" value="DASH_Dad1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28025">
        <property type="entry name" value="DASH COMPLEX SUBUNIT DAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28025:SF1">
        <property type="entry name" value="DASH COMPLEX SUBUNIT DAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08649">
        <property type="entry name" value="DASH_Dad1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="15" id="KW-0137">Centromere</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00030566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAZ85823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="56" mass="6046" checksum="8B17090C6E06B628" modified="2010-06-15" version="1">MNMNLLNRSLEGVVAVGREFESVEALWSQFEGVMGKNPDEAKTEGVGEGDDTRMGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNH0</accession>
    <name>D5GNH0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00011287001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430364">
        <property type="protein sequence ID" value="CAZ86063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841872.1">
        <property type="nucleotide sequence ID" value="XM_002841826.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNH0"/>
    <dbReference type="GeneID" id="9182263"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011287001"/>
    <dbReference type="HOGENOM" id="CLU_2529114_0_0_1"/>
    <dbReference type="InParanoid" id="D5GNH0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="13"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ86063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="84" mass="10007" checksum="6F8ED4F96258BB1B" modified="2010-06-15" version="1">MGQVEDNRGHTRSWHLCFFSFSFSSSPVFFSFFLFPLYIHIHSYSYSYSHLGSGFWCWFFLVLSPFLNLWEVFFSPLPVLDSTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNI2</accession>
    <name>D5GNI2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="1">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00011301001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="1"/>
    <dbReference type="EMBL" id="FN430365">
        <property type="protein sequence ID" value="CAZ86075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841884.1">
        <property type="nucleotide sequence ID" value="XM_002841838.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNI2"/>
    <dbReference type="FunCoup" id="D5GNI2">
        <property type="interactions" value="89"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GNI2"/>
    <dbReference type="GeneID" id="9186151"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011301001"/>
    <dbReference type="eggNOG" id="KOG0372">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004962_8_1_1"/>
    <dbReference type="InParanoid" id="D5GNI2"/>
    <dbReference type="OMA" id="LCEIICD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030289">
        <property type="term" value="C:protein phosphatase 4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051598">
        <property type="term" value="P:meiotic recombination checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000002">
        <property type="term" value="P:negative regulation of DNA damage checkpoint"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902660">
        <property type="term" value="P:negative regulation of glucose mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001034">
        <property type="term" value="P:positive regulation of double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903432">
        <property type="term" value="P:regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07415">
        <property type="entry name" value="MPP_PP2A_PP4_PP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047129">
        <property type="entry name" value="PPA2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45619">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="6">
        <location>
            <begin position="17"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ86075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="311" mass="35038" checksum="055453903CB645CE" modified="2010-06-15" version="1">MSDLDHAISLLRSCKPIPEPAVRELCYKARELLIEESNVQLVDAPVTICGDIHGQFHDLMELFRVGGDVPETNYLFMGDFVDRGFYSLESFLLLLSLKITTVYGFYDECLRKYGSANVWRYCCEVFDYLALGVNNGAVLCVHGGLSPLVDSVDKIRLLDRKQEVPHEGAMCDLLWSDPDEIDGWGLSPRGAGFLFGADIVKHFNHLNNLTLIARAHQLVMEGFKEMFDSSIVTVWSAPNYCYRCGNVAAILELGEERNGRRYRVFEAAPQDSRGMPAKKPVADYFLVRVYSTVWAVGHLLLTFSSNIMFRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNP1</accession>
    <name>D5GNP1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00011395001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430366">
        <property type="protein sequence ID" value="CAZ86134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841943.1">
        <property type="nucleotide sequence ID" value="XM_002841897.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNP1"/>
    <dbReference type="GeneID" id="9186210"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011395001"/>
    <dbReference type="eggNOG" id="ENOG502SBM6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1332779_0_0_1"/>
    <dbReference type="InParanoid" id="D5GNP1"/>
    <dbReference type="OMA" id="AMHETQL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037830">
        <property type="entry name" value="ZZZ3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22705">
        <property type="entry name" value="ZINC FINGER, ZZ DOMAIN CONTAINING 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22705:SF0">
        <property type="entry name" value="ZZ-TYPE ZINC FINGER-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="103"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="67"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="23"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="112"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ86134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="206" mass="22122" checksum="0455FEA719DE5634" modified="2010-06-15" version="1">MAAAGFTNPPRSPLLPPPHFSGSSIATAATATPSTSTSPSRTPSPRSPTLKPETNKNTDSIALSSALRLLILQRERAKRDIQILEQLRTEALDAPVEFMNALSLKSPPSPTSPTSSTSIPPSGVRLPKPQEIYKCPPIEWEKYRILPTPLERLHEEQKRLVPQQMQTGGVLGVGVEFGDTIAGGDGIQGRMRLFEGVGQRGGVVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNQ8</accession>
    <name>D5GNQ8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00011431001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DeSI family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430367">
        <property type="protein sequence ID" value="CAZ86155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841964.1">
        <property type="nucleotide sequence ID" value="XM_002841918.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNQ8"/>
    <dbReference type="STRING" id="656061.D5GNQ8"/>
    <dbReference type="GeneID" id="9182790"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011431001"/>
    <dbReference type="eggNOG" id="KOG0324">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069001_2_0_1"/>
    <dbReference type="InParanoid" id="D5GNQ8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0101005">
        <property type="term" value="F:deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1720.30">
        <property type="entry name" value="PPPDE domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008580">
        <property type="entry name" value="PPPDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042266">
        <property type="entry name" value="PPPDE_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12378">
        <property type="entry name" value="DESUMOYLATING ISOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12378:SF80">
        <property type="entry name" value="IP06716P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05903">
        <property type="entry name" value="Peptidase_C97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01179">
        <property type="entry name" value="DUF862"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51858">
        <property type="entry name" value="PPPDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPPDE" evidence="5">
        <location>
            <begin position="20"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="183"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="222"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="233"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ86155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="259" mass="28599" checksum="E19AD5A47A3AEB81" modified="2010-06-15" version="1">MPSLSRSSQSSARSQSLQKKKVIINVYDLLPPGRLSSIVWTLGVALLHSGVVINDREYAFGGHDRRGFTGVYWTKPKTEPPGGTFRSEILHGFTYASDQEIEQIIREASSEFLGPTYNLLTRNCNHFTSHLCVALTGRAAPAFLNRAASIGVALPCVVPAGWIEPPECEVADDWTDHDENARLTRNAGGASSASRRRSGDEAWESSNEGDCASDDGEAERQLRKRERIKRMKGRESVELRDTDGREVPAAGRARVSRRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5GP39</accession>
    <name>D5GP39_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosomal protein L19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00011662001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL19 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430372">
        <property type="protein sequence ID" value="CAZ86282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842091.1">
        <property type="nucleotide sequence ID" value="XM_002842045.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GP39"/>
    <dbReference type="FunCoup" id="D5GP39">
        <property type="interactions" value="924"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GP39"/>
    <dbReference type="GeneID" id="9186521"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011662001"/>
    <dbReference type="eggNOG" id="KOG1696">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083919_0_0_1"/>
    <dbReference type="InParanoid" id="D5GP39"/>
    <dbReference type="OMA" id="NRVWIDP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01417">
        <property type="entry name" value="Ribosomal_L19e_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1200.240:FF:000001">
        <property type="entry name" value="Ribosomal protein L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1650.10:FF:000001">
        <property type="entry name" value="Ribosomal protein L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01475">
        <property type="entry name" value="Ribosomal_eL19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035970">
        <property type="entry name" value="60S_ribosomal_eL19_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039547">
        <property type="entry name" value="Ribosomal_eL19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023638">
        <property type="entry name" value="Ribosomal_eL19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000196">
        <property type="entry name" value="Ribosomal_eL19_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015972">
        <property type="entry name" value="Ribosomal_eL19_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033935">
        <property type="entry name" value="Ribosomal_eL19_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057259">
        <property type="entry name" value="Ribosomal_L19e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057260">
        <property type="entry name" value="Ribosomal_L19e_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006343">
        <property type="entry name" value="PRK08570.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10722">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01280">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25476">
        <property type="entry name" value="Ribosomal_L19e_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01416">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48140">
        <property type="entry name" value="Ribosomal protein L19 (L19e)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00526">
        <property type="entry name" value="RIBOSOMAL_L19E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein eL19" evidence="6">
        <location>
            <begin position="26"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="181"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="181"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ86282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="218" mass="25354" checksum="1C4BEF65B5C19051" modified="2010-06-15" version="1">MQLWNLIMKFSRQTSKPSVPGVKMVNLRTQKRLAASVMGVGKRKVWLDPNEMNEISNANSRQTIRKLFKDGLIIKKPNTMHSRYRTRELAAAKRAGRHTGFGKRKGTKDARMPSQVLWMRRLRVLRRLLVKYRASGKIDKHLYHELYHLSKGNTFKHKRALIEHVHKAKAEKARERILKEEMDAKRARTKAARERKQARVQEKKNALLAGAEDEAADK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPE4</accession>
    <name>D5GPE4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">cyclin-dependent kinase</fullName>
            <ecNumber evidence="2">2.7.11.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00011694001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="2"/>
    <dbReference type="EMBL" id="FN430373">
        <property type="protein sequence ID" value="CAZ86306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842115.1">
        <property type="nucleotide sequence ID" value="XM_002842069.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPE4"/>
    <dbReference type="STRING" id="656061.D5GPE4"/>
    <dbReference type="GeneID" id="9186546"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011694001"/>
    <dbReference type="eggNOG" id="KOG0600">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_280208_0_0_1"/>
    <dbReference type="InParanoid" id="D5GPE4"/>
    <dbReference type="OMA" id="NRIITLW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008024">
        <property type="term" value="C:cyclin/CDK positive transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030332">
        <property type="term" value="F:cyclin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008353">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07840">
        <property type="entry name" value="STKc_CDK9_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000440">
        <property type="entry name" value="Serine/threonine-protein kinase bur1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000270">
        <property type="entry name" value="Serine/threonine-protein kinase bur1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF546">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="735"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="657"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1058"/>
            <end position="1122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="8"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="29"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="51"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="74"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="121"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="254"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="326"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="336"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="377"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="473"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="484"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="507"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="537"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="566"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="619"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="683"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1058"/>
            <end position="1082"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1084"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="764"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ86306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1122" mass="126546" checksum="3C9BDEE86EFF8E44" modified="2010-06-15" version="1">MSKSVKSSHRDSTRDIWRPNMGEAERGRGRPRSQSRERRRSPPAGPMKRRQGGDRDFRSPDRGSRPFDKPPPSSRRRSRERSRSGGRRSPRYESTSPKRPLRNEKTGGRHAGGGASESRHSNRYKRHRPSGSPNKRRRSRSPGHSTNRHSSKKTRRPRSLSRSRSVARRGKRDCSASPRPLYSPPPRKYKPPNRGPDNRDRSLSSSPRRSPRDFRFRSPIHRSIRGSPLPPSRRHSPPPSRGRSVSPMQPPPPRGRRGFRRNSRSPPPTGRGRRQFSSPQRHHNRSPRFQSPSYPQPPPEKKRSPNPLATLDKSPRPKAPYPSHSHARELGEHRTAVNSKPGNQPSVSNNDSRGGIVSAQDQSGPDAHEGQGPVEGSSKTFTPEKNTQKPAPPTAPHINDGPYVNPERQKNILAEDTPGFGRGRRFSREHPTGPSRGYHYQDGPPPGSRRGSQHSDYHAPFSGRGSRQGQFSPERRQSFEERRSGRRFGGRGHRGRGRGGGGGGGNVHDDGGMGRGRNRFDSHEQPPTHPRQSRPATPEDSRRPSSRSKDGSQEGHHEKEDDNGKMLTSRNSSVTRESNPQADHEMPPPSWPVSKNSPEPPPQDSRSFKRISGGPNANRAPTSGGSVSTGESKGASNFRPFALGLFKNKKSLPKALEVSREPHSRGPLRGSGVNNIPLGKNAEALKKPTKRETPLPPTASMSNYRETEQVPQVPETPQAHTVVRPQPPPAKESIYTRLSMVGEGTYGKVYKASNSVTKELVALKRIRMESERDGFPITAVREMKLLQALKQDNVVSLLEMMVEKSDFYMVFEYMDHDLTGILNHPTFRLEPCHIKHLAKQFFEGLEYLHHRGVLHRDIKGSNILLNNDGQLKIADFGLARFYTKASKKQLDYTNRIITLWYRPPEILLGATAYGPAVDIWSAACVFVELFTRQPVFTGKTEIDQLDTIYNVMGTPSEKIWPGLKETPWYGLLRTPARRRPKFQERYSSLLPDTAMELATQMLQYDPDKRPSAEEILKHQYFLEEPAPAPPLGLRDLKGDWHEYESKIERRREREINEKKKRALHEVDRRKWKEAQAEKKRLAQQEYNPEQPISATATKRKREDGDIEPSGSSGSVKKREVGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPH0</accession>
    <name>D5GPH0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">rRNA methyltransferase 1, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00011842001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430375">
        <property type="protein sequence ID" value="CAZ86413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842222.1">
        <property type="nucleotide sequence ID" value="XM_002842176.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPH0"/>
    <dbReference type="FunCoup" id="D5GPH0">
        <property type="interactions" value="210"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GPH0"/>
    <dbReference type="GeneID" id="9186653"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011842001"/>
    <dbReference type="eggNOG" id="KOG0838">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021322_5_3_1"/>
    <dbReference type="InParanoid" id="D5GPH0"/>
    <dbReference type="OMA" id="ERMITQS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016435">
        <property type="term" value="F:rRNA (guanine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18105">
        <property type="entry name" value="SpoU-like_MRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047182">
        <property type="entry name" value="MRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047261">
        <property type="entry name" value="MRM1_MeTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013123">
        <property type="entry name" value="SpoU_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46103">
        <property type="entry name" value="RRNA METHYLTRANSFERASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46103:SF1">
        <property type="entry name" value="RRNA METHYLTRANSFERASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08032">
        <property type="entry name" value="SpoU_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00967">
        <property type="entry name" value="SpoU_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="RNA 2-O ribose methyltransferase substrate binding" evidence="10">
        <location>
            <begin position="129"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ86413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="420" mass="47495" checksum="005C7240ECDF9DEB" modified="2010-06-15" version="1">MNTPQSARLLHKIPIKILYKPLHRSVSTDSAINRGLRRSAAADRYYGCQKDTREYDYSPEPAIYQNSPPPLDPTIRRVVARREGEPKGDYFELEPRRELSPRLDHEPPQFGYTPAPRRYIPKVNYDAEWIYGTSVVEAALRCKRRELYKLYVYAGDNRTPAARERDRDMKDLARTAGVEIVDEEDMGKMDSMSNSRPHNGYVLECLPIKRTPIIALKGVQEDRNFFTLEMARHYFDGRPVTEHYYDKIRCRDPKRRYPFVLMLDEILDPGNMGAILRSAYFLGVDAVTITARNCARLSPVCLKASAGAAELLPIFEHAKSERMITQSTENGWTVYAAIPPPTKNQARRDRYVYAPEVAEVVKEGPVVLLVGNEGEGLRPPLKKLAKFCVSLVKGPGTDSLVDSLNVSVATALLCSKFLGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5GQ68</accession>
    <name>D5GQ68_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nascent polypeptide-associated complex subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Alpha-NAC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00012220001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NAC-alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430382">
        <property type="protein sequence ID" value="CAZ86661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842470.1">
        <property type="nucleotide sequence ID" value="XM_002842424.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GQ68"/>
    <dbReference type="FunCoup" id="D5GQ68">
        <property type="interactions" value="518"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GQ68"/>
    <dbReference type="GeneID" id="9185241"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012220001"/>
    <dbReference type="eggNOG" id="KOG2239">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1541230_0_0_1"/>
    <dbReference type="InParanoid" id="D5GQ68"/>
    <dbReference type="OMA" id="THDHKHE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005854">
        <property type="term" value="C:nascent polypeptide-associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22054">
        <property type="entry name" value="NAC_NACA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.30:FF:000002">
        <property type="entry name" value="Nascent polypeptide-associated complex (NAC), alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.30">
        <property type="entry name" value="Nascent polypeptide-associated complex domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016641">
        <property type="entry name" value="EGD2/NACA0like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038187">
        <property type="entry name" value="NAC_A/B_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002715">
        <property type="entry name" value="Nas_poly-pep-assoc_cplx_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21713">
        <property type="entry name" value="NASCENT POLYPEPTIDE ASSOCIATED COMPLEX ALPHA SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01849">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01407">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51151">
        <property type="entry name" value="NAC_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NAC-A/B" evidence="8">
        <location>
            <begin position="48"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="19"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="33"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ86661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="174" mass="19474" checksum="F1E113B565CA742A" modified="2010-06-15" version="1">MSNPRIEDVTEDETKQAEVEDADSSDDSEAGEGEASGIPAGSSVAVHSRNEKKARKQIAKLGLKHVPGITRVTLRRPKNILFVINQPDVYKSPSSNTWIIFGEAKIEDLNSQAQANAAQQLQQHESAANIEADLYGGQRRKRKRMGKRSMKRGWKIRILSLLWPRQPFLESELV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5G3T9</accession>
    <name>D5G3T9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="5">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00003802001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 5">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="domain">
        <text evidence="5">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="5"/>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835061.1">
        <property type="nucleotide sequence ID" value="XM_002835015.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G3T9"/>
    <dbReference type="STRING" id="656061.D5G3T9"/>
    <dbReference type="GeneID" id="9188380"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003802001"/>
    <dbReference type="eggNOG" id="KOG0342">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_26_6_1"/>
    <dbReference type="InParanoid" id="D5G3T9"/>
    <dbReference type="OMA" id="RYMETAD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd17964">
        <property type="entry name" value="DEADc_MSS116"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="138"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="291"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="564"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="588"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="625"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="660"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="695"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="712"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="747"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="763"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="787"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="823"/>
            <end position="832"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ79182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="832" mass="91490" checksum="298CA3EE24A78074" modified="2010-06-15" version="1">MFSSTLRHHSSSIARISFISIRSLTSYTPTRTIVRNSLKNPPAASSQHRFFSVSPFLKNDTEAEQEFVEESAADPDPPAGEYIHHTGDPGVTHHYRKDEPITRFQQLADRGLVDPVIIDKIVKGKGYEEMTKVQRKTILETLGGDDVFGQAKTGTGKTFAFLLPVIQRIISTTIPLKPRMRIDFRTRNGETNADVRAIIISPTRELAQQIKVDAQFLTQNTEIVTQLAVGGSGKRESLANIRSVGCHILVATPGRLHDLLSDPLAGISTKNLEILVLDEADHLLDQGFAEAIDDIIKLLPNRDAPVGNTGKFRQTLLFSATIPDKVQKMARKTMRHGHKFLQMLLKREIEIVDNSVGTEKPLQPFKAIIFFTAVRPTFEMHSRLSQYQRTRTAESFRRARSGILLSSDITARGMDFPGVTHVIQMGAPKNEETYIHRIGRTARGDRSGVGYLFLSGMEYPDAMSELRKLPLKLNNSLVTAGIDLGVEQELPKHAAEILMSVTEANKKIDPYTMKDTYMSLIGCYGGIKKPVLAESLNRLATLGWGLPSPPEVSRVLASKLGIGSHHGFHVSTSTRGEADSEESDRPSRGGGGGRGGRGGFSGRGGRGGFSDRPSYRSSGGEEGGGYERKPFRPRDDNNGSGYERKPFRPQENGDGEGDGGYERKPFRPRDDNNGSGYERKPFRPQENGDGEGDGGYERKPFRPRDSNNGSNYERKPFHSRDDSSGGYERKPFRPRNDDDDEGGSGNYERRPYRSRSDDDDGEGGGGGGGYGRGSFAPRGSRGRGRGRGGFGGRSSRGSGGERGHGGYQRARNSGPGMWERGNRGRSSRGGRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4N8</accession>
    <name>D5G4N8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00000029001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429989">
        <property type="protein sequence ID" value="CAZ79481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835324.1">
        <property type="nucleotide sequence ID" value="XM_002835278.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4N8"/>
    <dbReference type="STRING" id="656061.D5G4N8"/>
    <dbReference type="GeneID" id="9188417"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000029001"/>
    <dbReference type="eggNOG" id="ENOG502S4E9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_328503_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4N8"/>
    <dbReference type="OMA" id="KFSPSPW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048401">
        <property type="entry name" value="SLS1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032741">
        <property type="entry name" value="Sls1_KH-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048748">
        <property type="entry name" value="SLS1_KH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048400">
        <property type="entry name" value="SLS1_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14611">
        <property type="entry name" value="KH_SLS1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20777">
        <property type="entry name" value="KH_SLS1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20778">
        <property type="entry name" value="SLS1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20776">
        <property type="entry name" value="SLS1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SLS1 N-terminal" evidence="3">
        <location>
            <begin position="104"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="SLS1 first KH" evidence="2">
        <location>
            <begin position="196"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="SLS1 second KH" evidence="4">
        <location>
            <begin position="261"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="SLS1 C-terminal" evidence="5">
        <location>
            <begin position="351"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="845"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="849"/>
            <end position="863"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF20778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ79481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="875" mass="98136" checksum="30FD84C118D5C723" modified="2010-06-15" version="1">MPPASYSSNLATCLCRQFRRPSLNIRILQAYARHFNTSPRKRGEEKEKESNKIDIGPISELLILRNLSPRKPRHESLQFLRDPLEAEALDDTQDLLLRSDDLESDKNIDIALTRPANVLVSKQRYEQLAQELCKAFLKPQLYEYYYYQDGEERDLPRLPSGANKREIIDGILSAKWKVVVSHEIPERLDVLVNGYFPSTRRDIFFILGKDGRVIRQLSQEFQARVRVNVGTGKIGVYASVENFERLKEAVQGLLGKVTVEEFDMSWAQRLCQFNKQFITPIARITGTYIEKKDEKTLLISSPDPSSLQDARRLLFLSFDLQLRSSHSLLYNAPKGLGDPKGALYPVHEKSALPWNFREVEWGRWRNIKKPIERSNSLRGESSAILKDTLRTRIGGTLQDTDITTGLREILDHNDLQVSAQHRSELLPKYEAILGYLLHEDSDPQPDQDLTVLEFIESKYRLQVLLTDVPGLVHITQGLSPISDPTSEGALHSLRVQGSTGVQQTPYQTCIVKLLPSPWEYPEGFDRYPPLELKFSIDPELGRVDQPSLKAIHSSTVADIMLPGEECDVRFRHQKSIPLGILGDPANPQSKYGVTQSELRRYMSESTLNPLTDDKLKASQLLSVTLPEWMISPPKRSDSKDGGERRPPTKMLYISTGLEYRRELAFDWNGMKLYQTVIQGGITGGRRSEFRLCWDNTEGAGSVAEATRITELEGDKEIDEIAKGSRYKLAVGAASEAVAEIVVAADTEAADGIPSKPAAGTGAKDVGEFGDRITKPTVDQSLQRAPQGPYYCSPSTPQSSFADFLTNTGDLVNRLGEFIASKEWRFTTQYTGHGVVRGVTRSVKRATTSRGDRGVRSGECERGSGHGGRSCATRVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4Z9</accession>
    <name>D5G4Z9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00000215001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429994">
        <property type="protein sequence ID" value="CAZ79592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835435.1">
        <property type="nucleotide sequence ID" value="XM_002835389.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4Z9"/>
    <dbReference type="GeneID" id="9186300"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000215001"/>
    <dbReference type="HOGENOM" id="CLU_821806_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4Z9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="287"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="287"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="328"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="338" mass="37726" checksum="62D0CCF6D7ED1E99" modified="2010-06-15" version="1">MPTAATSVAETTTPEPAKKRACPPKNCPCEWCGVVFSKSGLGRHYDYWVYSASPGKPDEKHTLEAIQRMREIRKDARRLKGCDKLDPKEIKSRQNKRNYERHKGRHVEKKRAKRMSEMARSMVLKEIAMEISAISAPPTYPTLEPDSEALVNFPALVYAFLSPPSDVFAPSPNRNLSSWGDQLPGATEYVALGVKFKQPDLSKWSQRLDQEWQNWGSLSELEKSKLCFQEIGRALSRSQADAATYKPYARILGKLGGEELGNRIIKWEGRATQLRAQKGRAEELQLEKEWMATRDDQHLSHDNAPTEGGASGSGEGNWDNGRRGGSDSGTSSSGDDTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5G548</accession>
    <name>D5G548_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00000253001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429996">
        <property type="protein sequence ID" value="CAZ79633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835476.1">
        <property type="nucleotide sequence ID" value="XM_002835430.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G548"/>
    <dbReference type="STRING" id="656061.D5G548"/>
    <dbReference type="GeneID" id="9186331"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000253001"/>
    <dbReference type="eggNOG" id="ENOG502SR70">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057358_0_0_1"/>
    <dbReference type="InParanoid" id="D5G548"/>
    <dbReference type="OMA" id="RGMRHPW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046464">
        <property type="term" value="P:acylglycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050266">
        <property type="entry name" value="AB_hydrolase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43798:SF33">
        <property type="entry name" value="HYDROLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G14860)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43798">
        <property type="entry name" value="MONOACYLGLYCEROL LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="41"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ79633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="272" mass="29719" checksum="60B27F4D4C139231" modified="2010-06-15" version="1">MGSQPDATTAGGIELQTSPAENTRPKPLFFTERPSISPTAPTFLLLHGLYSSHLEFAHLPIPSTSYHILTPDIPGHSQSSHLPVSLQNTTSLLADLIKSHAHSSRAHIAGVSYGAYLALHLAATHPDLVDSVFVTGCTLPTSVFLGGLSWMANVPLLYFPNVVNYALSWYGGTISEELLRDMHTNATMALGNAAYASLKDWELKPLPMRLRGMERRVREGNAESRAVVAKGKILAWDIQAPEDFAEALMAWAEGRGMPGFIISLEKWFSWGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5R8</accession>
    <name>D5G5R8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00001451001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M28 family. M28B subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430001">
        <property type="protein sequence ID" value="CAZ79861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835704.1">
        <property type="nucleotide sequence ID" value="XM_002835658.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5R8"/>
    <dbReference type="FunCoup" id="D5G5R8">
        <property type="interactions" value="18"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G5R8"/>
    <dbReference type="GeneID" id="9187539"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001451001"/>
    <dbReference type="eggNOG" id="KOG2195">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005688_1_0_1"/>
    <dbReference type="InParanoid" id="D5G5R8"/>
    <dbReference type="OMA" id="YPRKDGR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08022">
        <property type="entry name" value="M28_PSMA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02121">
        <property type="entry name" value="PA_GCPII_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000101">
        <property type="entry name" value="N-acetylated alpha-linked acidic dipeptidase like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.40">
        <property type="entry name" value="Transferrin receptor-like, dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039373">
        <property type="entry name" value="Peptidase_M28B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007365">
        <property type="entry name" value="TFR-like_dimer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036757">
        <property type="entry name" value="TFR-like_dimer_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404:SF71">
        <property type="entry name" value="CARBOXYPEPTIDASE TRE2, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G10650)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404">
        <property type="entry name" value="N-ACETYLATED-ALPHA-LINKED ACIDIC DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04253">
        <property type="entry name" value="TFR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47672">
        <property type="entry name" value="Transferrin receptor-like dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PA" evidence="3">
        <location>
            <begin position="305"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="5">
        <location>
            <begin position="484"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="domain" description="Transferrin receptor-like dimerisation" evidence="4">
        <location>
            <begin position="734"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="26"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ79861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="858" mass="95490" checksum="328183102F2DEA6D" modified="2010-06-15" version="1">MSDEKRSMLGSSLPIPTYEEATSSRAHSDVGDNPHTNEEASHLLASSYGRYRQPTVESARSSLESSFLEEFEDSVRPSRESLERVMVEMEILEPGQGEEGQRRGGGRLRMHISKRISSITDSLSAISIPHHWRINPFRWFSCIRIPDLPCFCEGIVPLYRVIGVIVGLIVVYALLASDSVTLSPPPGASGIVEFDINALKDFIRDSIDRDNIAHYLEYLSRFDHTAGTEGDFVLAKYVEGEFRSFGLESVGLDKYSVYLNFPKPNGRKLAIIDPPWEAALEEPKANGDRENTLVFHGYSRTGSATGPLIYANYGARADFDKLESQGLNLTGSIILVRYGGTQMDRALKVKAAEERGAAAVIEFSDPKTEGWDLPEGFVQRGSVSLMSFMVGDVLTPGTPSKPGYDRIPLEHNPGLVGVPSLPLSWDNARHLLQSLKGKGSKVEESWTGDTRGVEEWWTGSINDSSVVVKLENDQVEEKRHPIWNVLGYIRGSDDHTKRIVVGNHRDAWCFGASDPNSGTAVMLEVARLFGKLLEMGWRPQRSIIFANWDAEEYNLIGSTEYVEDNIDDLRLHGMAYINLDVAVSGREFRAAGSPLTQQPLFNVLERIQSPSGNGTLRGDWGDKFLPGLGAGSDYVAFQDYAGVSSIDIGFAGDPFPYHSCYDTFEWMKNTGDPGFIHHLVIAQVLGVLIIELADTPVLPFNMVDYAHALGRYTDDLEEHARKVMRTQSPDTKPLDFKDLRHAVESAEKRLKEFDEKHENWMDYVGTGLDTNGIIARQSRNARMSNFEEHLLDLSKGGGLPGREWFKHVVFAPEAWSGYDAGYFPGVRDALAGKDWELAQKQVEKIANVIDKASYKLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5T5</accession>
    <name>D5G5T5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00001471001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ADIPOR family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430001">
        <property type="protein sequence ID" value="CAZ79878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835721.1">
        <property type="nucleotide sequence ID" value="XM_002835675.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5T5"/>
    <dbReference type="FunCoup" id="D5G5T5">
        <property type="interactions" value="331"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G5T5"/>
    <dbReference type="GeneID" id="9187556"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001471001"/>
    <dbReference type="eggNOG" id="KOG0748">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023075_2_0_1"/>
    <dbReference type="InParanoid" id="D5G5T5"/>
    <dbReference type="OMA" id="IGNACDY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006882">
        <property type="term" value="P:intracellular zinc ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004254">
        <property type="entry name" value="AdipoR/HlyIII-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855:SF52">
        <property type="entry name" value="ADIPONECTIN RECEPTOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855">
        <property type="entry name" value="ADIPOR/PROGESTIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03006">
        <property type="entry name" value="HlyIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="116"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="171"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="205"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="237"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="273"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR604254-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ79878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="306" mass="33455" checksum="029D3631CF864A03" modified="2010-06-15" version="1">MAVKNRNTQRGGAGGGSAGEMVVEGAKNAEKKVEEALTVLLHELPLWMQDNHFIVSGYRPQSFSFYKSFHSLTYLHNETVNIYTHLLPALLFLLLGTYTVKTHLLPRYPTSTAPDLLSFACFFASASTCLGILAVARVGNKLDYLGIIILIMGSFIPSVHYAFYCRRRLAYAYWSMISLLGAGCAAVSVMPGFRTPAWRPFRAGLFVSMGLSSVLPVLHGVRIYGTRGMDERMGVRWLLIEGLLYIAGAGIYAARVPERFAPGRFDLLGASHQLFHCLVVAAAGSHLVGLVKGFDYLHSGDAQVCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5V5</accession>
    <name>D5G5V5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="11">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00001592001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430002">
        <property type="protein sequence ID" value="CAZ79898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835741.1">
        <property type="nucleotide sequence ID" value="XM_002835695.1"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G5V5"/>
    <dbReference type="GeneID" id="9187576"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001592001"/>
    <dbReference type="eggNOG" id="KOG4226">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_320592_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5V5"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    </dbReference>
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        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03983">
        <property type="entry name" value="SHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
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        <property type="match status" value="3"/>
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    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="10">
        <location>
            <begin position="4"/>
            <end position="70"/>
        </location>
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    <feature type="domain" description="SH3" evidence="10">
        <location>
            <begin position="71"/>
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        </location>
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    <feature type="domain" description="SH3" evidence="10">
        <location>
            <begin position="402"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="126"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="467"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="135"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="200"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="224"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="277"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="476"/>
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            <begin position="529"/>
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    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="905" mass="99588" checksum="EA3B18CAF2DDDC02" modified="2010-06-15" version="1">MTSPFLGVYTAVYAYTPRDDQELTLEEGDLLYILEKSSDDDWWKAKKKSTGEEEEPIGLVPVTYIEEADPVGKSKALYSYTRQTDEEVSFAEDATLDVYDDVSDPDWALVGYNGEFGFAPTNYIEKIGAGESNPPASPTAVSPPSPIQAPAATSKHVSFSSPPREDTPPPPTPPRPQSQEYSPTQSPAANVATVMHQRIPQHSVSSPRSPTSGMASPTGSAPRSYKDRNQERSHEPSHDRSREQRSKQEQKYIHDLSDSEDGDGPRIPFRRPGQPRHGVEGGHSHGRDSPPIPPGFQTYPVQEVDSRKKRAATLGLGPTRIILLPDKSTRPKEEWSVENLKGYNVEGKHVFLELEKPSRSLDLHAGSKDTAEQIVSALSEIRGAVKAAGISEVFAAASGGGKRNDIGTVLCDFAAQGEDEVSVTTGDEVLILDESNDEWWLVRRQVNGLEGVVPASFIERGRSKVVTPGIMASPSDPKERIKMQEHGGDPRASGIPQRTSSLAGSHSRDDRRRRRPDSIASDQAGSGKQKPDSSRIRQWTDRTGNFKVEAQFIGCKDGRTGAIQVACRRGITXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSNNRHLFLNRLELASGALISQALHRCLVNSECSSQMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5W4</accession>
    <name>D5G5W4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00001601001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430002">
        <property type="protein sequence ID" value="CAZ79907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835750.1">
        <property type="nucleotide sequence ID" value="XM_002835704.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5W4"/>
    <dbReference type="STRING" id="656061.D5G5W4"/>
    <dbReference type="GeneID" id="9187585"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001601001"/>
    <dbReference type="eggNOG" id="ENOG502S77X">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_960399_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5W4"/>
    <dbReference type="OMA" id="HQTNIDK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="71"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="110"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="71"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ79907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="290" mass="33533" checksum="88B46B71598E0967" modified="2010-06-15" version="1">MNGNTYPPELEFLDVPSREDSVLPVPNGDSSCDAPGSGGANLSRITTGKSGRVIERLMSDVDRLKRELKVETTAREEERKAKEAIKSSRDSLQSTNDNLIHQTNIDKASLARKDRKIEELKAEREAERARRLESDSSLNEHIRQSNEQLQELKTQLSHEVVERKRASNQYEVLLQSWKHLDEGYTGRVDKLREELEELQKGRLKDRELFRRLEVTIEQQQQELEKLRFAKNRISDKYLKVIHEAEGEIEDIRTLAKVAEKETAETLTEAKDTLGQLRHVLNVQRDLRKDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5X6</accession>
    <name>D5G5X6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00001613001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430002">
        <property type="protein sequence ID" value="CAZ79919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835762.1">
        <property type="nucleotide sequence ID" value="XM_002835716.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5X6"/>
    <dbReference type="STRING" id="656061.D5G5X6"/>
    <dbReference type="GeneID" id="9187597"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001613001"/>
    <dbReference type="eggNOG" id="ENOG502S96D">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064111_1_0_1"/>
    <dbReference type="InParanoid" id="D5G5X6"/>
    <dbReference type="OMA" id="TDIMIRY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00076">
        <property type="entry name" value="HFD_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006565">
        <property type="entry name" value="BTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037818">
        <property type="entry name" value="TAF8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46338">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46338:SF1">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Bromo_TP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
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        <location>
            <begin position="4"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="112"/>
            <end position="143"/>
        </location>
    </feature>
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            <begin position="112"/>
            <end position="127"/>
        </location>
    </feature>
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        <location>
            <begin position="134"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ79919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="143" mass="15644" checksum="E4587943020F6040" modified="2010-06-15" version="1">MTDSEVYFTILRVSAAQTLRSAGITAAKPSVVDAFTDLLARYLTLLGTTTRNFAESGGRTQAELIDARMAMEHVGLLRPINIFSNPDDDDTEAVDALVEWFRGPQAADMRRVAGHAEKEGQVGKSDEWLGATKKLSEKRNTTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5G600</accession>
    <name>D5G600_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00001700001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430005">
        <property type="protein sequence ID" value="CAZ79943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835786.1">
        <property type="nucleotide sequence ID" value="XM_002835740.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G600"/>
    <dbReference type="GeneID" id="9187621"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001700001"/>
    <dbReference type="HOGENOM" id="CLU_2529105_0_0_1"/>
    <dbReference type="InParanoid" id="D5G600"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="84" mass="9548" checksum="92976A49DD5F0626" modified="2010-06-15" version="1">MRQHSKLHLPLPKPPSEDHEPRQGSEEEKKNRWIQNPAIGLPPTQSPSPMSRPRAQGPIFHPITQQRPTYITPVIEEREGGEDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6C9</accession>
    <name>D5G6C9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">20S-pre-rRNA D-site endonuclease NOB1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00004426001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for the synthesis of 40S ribosome subunits. Has a role in processing 20S pre-rRNA into the mature 18S rRNA, where it is required for cleavage at the 3' end of the mature 18S rRNA (D-site). Accompanies the 20S pre-rRNA from the nucleus to the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
            <location evidence="7">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the NOB1 family.</text>
    </comment>
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        <property type="protein sequence ID" value="CAZ80072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002835869.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6C9"/>
    <dbReference type="FunCoup" id="D5G6C9">
        <property type="interactions" value="975"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G6C9"/>
    <dbReference type="GeneID" id="9181157"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004426001"/>
    <dbReference type="eggNOG" id="KOG2463">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024666_2_0_1"/>
    <dbReference type="InParanoid" id="D5G6C9"/>
    <dbReference type="OMA" id="GYELECE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd09876">
        <property type="entry name" value="PIN_Nob1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000020">
        <property type="entry name" value="20S-pre-rRNA D-site endonuclease NOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.210.10">
        <property type="entry name" value="Nin one binding (NOB1), Zn-ribbon-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039907">
        <property type="entry name" value="NOB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017117">
        <property type="entry name" value="Nob1_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036283">
        <property type="entry name" value="NOB1_Zf-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014881">
        <property type="entry name" value="NOB1_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033411">
        <property type="entry name" value="Ribonuclease_PIN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12814">
        <property type="entry name" value="RNA-BINDING PROTEIN NOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12814:SF2">
        <property type="entry name" value="RNA-BINDING PROTEIN NOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17146">
        <property type="entry name" value="PIN_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08772">
        <property type="entry name" value="Zn_ribbon_NOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037125">
        <property type="entry name" value="D-site_20S_pre-rRNA_nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144206">
        <property type="entry name" value="NOB1 zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Ribonuclease PIN" evidence="11">
        <location>
            <begin position="12"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="Nin one binding (NOB1) Zn-ribbon-like" evidence="10">
        <location>
            <begin position="296"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="115"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="179"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="390"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="129"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="211"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="390"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="321"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="324"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR037125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR037125-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ80072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="441" mass="48266" checksum="2A2C1C0C5C688F80" modified="2010-06-15" version="1">MLTEDQTPIDTLVIDASPLIRNDFTTSILNRVGKLYSTPAVIAEIRDVATRSRLETTWLPLLALRTPKPESVKVVSDFVKKTGDFPVLSVTDLQLLALTYELEVELNGGNWRLKNEPGQKKINGPVPKPARESEEGKKVVEEEGVANDREKTEDGGDAETAGVIDISAASAEDDLSQKLDNTHILPDKIAPTEASGSTERASSPPPGPIANEDSNPPSSTSTSSEDSDSDADGWITPSNLHKHQSKSTTSPPPSSTSPTKNPWPIRAALATTDFALQSVTLQMNLHLLSTKTLQRIHTIRSHVLRCHACFKLTRDMAKQFCPVCGGPTLQRVSCSADSKGAFKIYLKHNYQWNNRGSVFSLPKPTHGSANGKGGREVLILREDQKEYEREVARGERRKERDLLDPDYLPGILTGERRDAGGRPRIGYGKRNPNVAKKGGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6E0</accession>
    <name>D5G6E0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00004442001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430009">
        <property type="protein sequence ID" value="CAZ80083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835926.1">
        <property type="nucleotide sequence ID" value="XM_002835880.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6E0"/>
    <dbReference type="STRING" id="656061.D5G6E0"/>
    <dbReference type="GeneID" id="9181168"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004442001"/>
    <dbReference type="eggNOG" id="KOG0920">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001832_1_1_1"/>
    <dbReference type="InParanoid" id="D5G6E0"/>
    <dbReference type="OMA" id="MCKLVCR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17917">
        <property type="entry name" value="DEXHc_RHA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000002">
        <property type="entry name" value="Putative ATP-dependent RNA helicase DHX36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF203">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="6">
        <location>
            <begin position="416"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="556"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1108"/>
            <end position="1236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1227"/>
            <end position="1236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ80083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1236" mass="137631" checksum="D3AD880F6966380F" modified="2010-06-15" version="1">MRGPSLKTEIVDDIWLTKNFEQPQPDDYKSAPETLFTNPKTFLWDYKGIAAKSSFASHKGGIYRCTVIATLPDRTKIEACGDAKNKKIAEKSACRHLVLKLHQNGMLQGIVNNTESAGVHDKELLSDEVDAKMDIYDYCARFDAVPTVNVRETKGPGPRKGLFEVTVGFPQQNILATARASELKNAEIVACVEFKRQAEEFHSKQGGSTIILKDISSINSRNARKFFEFFKVFDRQARYDVELKQTSGRRGSGGAFRAQMMLNEEPLGEPVEFVGKKYAETAAFLTGAIALKQIRPELFPKFIEALRLGNGELLKPLLPSWINIDGDCISTMTDTLHAVRRIGMPPSQDEEDWMEQEQARNSSRFGPRKALDPAFVGVKSKKLLESYEEYLNDPKLETLRSKREELPMNQYRAKVLDIVNNNAVSIIVGATGSGKTTQVPQILLEEAIKNGTGANCNIICTQPRRIAATSVAARVAVERNEPLQKSIGYQVRFDSKLPIPGGSVTYCTTGILLQQLRNSADEALDGITHLVIDEVHERDILIDFLLIILKRVMHDRMKRGLPEVKVVLMSATMDTELFASYFQYKTADGRLVPCPNLSVPGRTFPVKEVYLEEIQDALHALLSMLHSSIPNQNEVFEDVLEGRRKVILSTNIAETSVTIPDVRYVVDSGKLREKQYEQARRITQLVCTWISKSNSKQRAGRAGRVQNGNYYALFSKSRSDSLRGTGLPEMLRSDLQEICLDIKAQGFKDSVAQFLSEAIEPPSRNAIEASLAQLRLLGALTENEGLTPLGRVLATMPVEPALGKMILLAVIFRCLDPIMILGSSSGVRDIFVAPPERRAEANRVRNSFVRDTGSDHMALINAFREWRSIRDRDGMHAAHRFAEENFLHRGALRTLDATTLQIEELLVKANIIPHVRRYERNRSEIGHPSSNQYSDHVPLIKALTLAGMYPNIAVASGGKGLRTPNENFSMIHPTSVHYTNRSDSGMPFGTLVTFTTKAKSNDGGTLLLRTVTEARPLMAILFGGKLESSGTIVEIDGWLPFQVPPPAVKKTNEFRMCLDRLLGHAFRSLSERNLRGNNFLADNRAREAFAEGLVELLKIDSQQITRRAVSAERERGSASGHGRNQPWDTGRQAFGGNRNQSFDRRNQPRGRGNSGIGFARRDQPFDRGNSGERFSSQNPMPRDRSLDRSSFGQDPAPQRGGGNSGSSFQRGSGFRRELSAGGRSDWGFNSSSGRRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5G797</accession>
    <name>D5G797_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00002523001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M20A family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430022">
        <property type="protein sequence ID" value="CAZ80390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836199.1">
        <property type="nucleotide sequence ID" value="XM_002836153.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G797"/>
    <dbReference type="STRING" id="656061.D5G797"/>
    <dbReference type="GeneID" id="9185288"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002523001"/>
    <dbReference type="eggNOG" id="KOG2275">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021802_3_0_1"/>
    <dbReference type="InParanoid" id="D5G797"/>
    <dbReference type="OMA" id="RLHKGVM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05652">
        <property type="entry name" value="M20_ArgE_DapE-like_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001261">
        <property type="entry name" value="ArgE/DapE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050072">
        <property type="entry name" value="Peptidase_M20A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43808">
        <property type="entry name" value="ACETYLORNITHINE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43808:SF8">
        <property type="entry name" value="PEPTIDASE M20 DIMERISATION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00759">
        <property type="entry name" value="ARGE_DAPE_CPG2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072001" evidence="6">
        <location>
            <begin position="20"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="7">
        <location>
            <begin position="213"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ80390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="427" mass="46145" checksum="473D6BDADE1CD6E9" modified="2010-06-15" version="1">MKLIDPASLGLLLLPFTQSLQLPFGIPTSVRGIRSTLGASFKVPEFIKKFADPISTSPLYSLHRSLIEIPSITGNEKGVGEWLAGYLMGKNFTVETQAVSEDRVNIFAYLGKNRTTHTLVTSHIDTVPPFIPYKATSNTIYGRGSNDAKGSVASQITAVEELIAEEVIAEGDVSLLFVVGEETLGDGMKTANSLGPRWKTVIFGEPTENKLAVGHKGIVMFDVIAKGKASHSGYPQLGVNANSHLVEALYKIEHMILPRSDILGESTVNIGLIQGGVATNIISPHANASVLIRLAGDLDETLEVIKKTVYGLPVELRFVGVAYGPQETDHDVENFETIVCSYGTDVPNLYGGHKRYLYGPGSILTAHSDSEYVLKSDLTEAVAGYKRLIQESLWPTKRVPAIIEKVIKEETVIAPVSRSATVGDKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7C7</accession>
    <name>D5G7C7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00002444001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family. Cyclin C subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430023">
        <property type="protein sequence ID" value="CAZ80420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836229.1">
        <property type="nucleotide sequence ID" value="XM_002836183.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7C7"/>
    <dbReference type="STRING" id="656061.D5G7C7"/>
    <dbReference type="GeneID" id="9185318"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002444001"/>
    <dbReference type="eggNOG" id="KOG0835">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022000_5_0_1"/>
    <dbReference type="InParanoid" id="D5G7C7"/>
    <dbReference type="OMA" id="WEDVWSV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043198">
        <property type="entry name" value="Cyclin/Ssn8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10026">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036580">
        <property type="entry name" value="Cyclin_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="2">
        <location>
            <begin position="39"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ80420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="293" mass="32533" checksum="AECC95EC21EB76D6" modified="2010-06-15" version="1">MVSLLRNPLATADQLASSPSQADGIPADLETSLRWAGSELIQAAGILLRLPQKTVATAIVLFQRFYLLSSMRSCAITDSCHAVIFLSSKLTEHPATPRDIINVTTYLLNTPSPSPISPAPAAGQGSTPPEEHYVTEQEYYIRRTRLLNVEMEILKACGFQTHLSLPYTLCINYIQSLSVLSAEVVKKSFGYLTDALLSPSLLYLTHQPNSLAVAAVYIGARECEVKLPAAWWDVFDCEREELGFLVVCMKGVKEFVESERKLWSGRKPGVPWELDDLEIHLERRRVLDGEAEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7F0</accession>
    <name>D5G7F0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Translation machinery-associated protein 20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00002534001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in translation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TMA20 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430026">
        <property type="protein sequence ID" value="CAZ80443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836252.1">
        <property type="nucleotide sequence ID" value="XM_002836206.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7F0"/>
    <dbReference type="FunCoup" id="D5G7F0">
        <property type="interactions" value="507"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G7F0"/>
    <dbReference type="GeneID" id="9188477"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002534001"/>
    <dbReference type="eggNOG" id="KOG2523">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090468_0_1_1"/>
    <dbReference type="InParanoid" id="D5G7F0"/>
    <dbReference type="OMA" id="GVENIHY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd11609">
        <property type="entry name" value="MCT1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21155">
        <property type="entry name" value="PUA_MCTS-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.400.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016437">
        <property type="entry name" value="MCT-1/Tma20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041366">
        <property type="entry name" value="Pre-PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22798:SF0">
        <property type="entry name" value="MALIGNANT T-CELL-AMPLIFIED SEQUENCE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22798">
        <property type="entry name" value="MCT-1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17832">
        <property type="entry name" value="Pre-PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005067">
        <property type="entry name" value="Tma_RNA-bind_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PUA" evidence="4">
        <location>
            <begin position="92"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR005067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ80443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="183" mass="19978" checksum="8C8FB8839E89818D" modified="2010-06-15" version="1">MLTAICRFTGSSKTNLKSSVQRSLRAKLVEQFPLLAPHIDEILPKKSQLSLLKAPERLSLYALGNEVLFFQHHDDALFPSLRLVHKFPNCFPTVQVDRGAIRFVLAGAMLMCPGLTSPGARLPAEGEGYNAGMVVAVNAEGKENACMVGMLKMGTDEIKKVNKGIGVETVHYLGDGLWRLDAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7K6</accession>
    <name>D5G7K6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00004599001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430031">
        <property type="protein sequence ID" value="CAZ80499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836308.1">
        <property type="nucleotide sequence ID" value="XM_002836262.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7K6"/>
    <dbReference type="GeneID" id="9186978"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004599001"/>
    <dbReference type="HOGENOM" id="CLU_2147696_0_0_1"/>
    <dbReference type="InParanoid" id="D5G7K6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ80499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="112" mass="11860" checksum="2F454B59C950C1FB" modified="2010-06-15" version="1">MSECGLRECAFDRVAIQQTVIFGYQVCSKKGFVEFPYADDLASSLGIEVSANTTLPGRITIAANGVGGAPSFPILDEDGDRMVFGSAAWKILPEGSILVPALLLTAGFMTVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7M5</accession>
    <name>D5G7M5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00004630001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430031">
        <property type="protein sequence ID" value="CAZ80518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836327.1">
        <property type="nucleotide sequence ID" value="XM_002836281.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7M5"/>
    <dbReference type="STRING" id="656061.D5G7M5"/>
    <dbReference type="GeneID" id="9186997"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004630001"/>
    <dbReference type="eggNOG" id="ENOG502QRX5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019533_1_2_1"/>
    <dbReference type="InParanoid" id="D5G7M5"/>
    <dbReference type="OMA" id="WSDSFHN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050546">
        <property type="entry name" value="Glycosyl_Hydrlase_16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963:SF62">
        <property type="entry name" value="GLUCAN 1,3-BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963">
        <property type="entry name" value="GLYCOSYL HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00722">
        <property type="entry name" value="Glyco_hydro_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="3">
        <location>
            <begin position="124"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ80518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="450" mass="50535" checksum="C54E34D934DAC1FA" modified="2010-06-15" version="1">MDGTHAQATASSSAMPATPLPSPFVDPITPLGSTPYSSRPGSLPGSTSGFSAPGLNVPQRYFHSRRVRPGEAQQPWKGKKDPKEKWVTIIPCIGLAVGLAIAGFFVWHGVSSVVNHKYCPVLIEDWSNGFDEKIWTKEVECGGFGNGQFDYTTNSEENSYVKGGHLHIRPTLQDEKLITTDNVMNLTEMGICTSPLLKNCVSGTNSTNGTIINPVKSARINSRKGAKIKYGRVEVKARFPEGKWLWPAIWMLPVEEKYGPWPASGEMDIAESRGNDWKYAQGGNDIISSTLHWGPDPANDAWWRTNVKHRASHTSYSSSFHTFGIEWSEKYIFTYVDSRLLQVLYTNFQTRLWDRGNFPLASENGTRYIDPWSQTGRENTPFDQEFYLIISLGVGATSGWFENGKDKKPWVDGSPTAVKDFWDARDEWKKTWKNDGEMVIESVKMWQQCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8B5</accession>
    <name>D5G8B5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00002963001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430041">
        <property type="protein sequence ID" value="CAZ80758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836567.1">
        <property type="nucleotide sequence ID" value="XM_002836521.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8B5"/>
    <dbReference type="FunCoup" id="D5G8B5">
        <property type="interactions" value="35"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G8B5"/>
    <dbReference type="GeneID" id="9188360"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002963001"/>
    <dbReference type="eggNOG" id="KOG1938">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014185_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8B5"/>
    <dbReference type="OMA" id="PHMENRV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990072">
        <property type="term" value="C:TRAPPIII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024420">
        <property type="entry name" value="TRAPP_III_complex_Trs85"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12975:SF6">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12975">
        <property type="entry name" value="TRANSPORT PROTEIN TRAPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12739">
        <property type="entry name" value="TRAPPC-Trs85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="405"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="648"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="405"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="416"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="658"/>
            <end position="668"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="708" mass="78548" checksum="1B70B8E7F2EC9CEC" modified="2010-06-15" version="1">MSSAFPSLRTRPTLPAVGVSRATSVYLRSSSIAPVHSSVTSLNSLFNQASLSSPRSPEDEDCKGLVCRAFVPYVAVHVSDDTNELAREKGFVDFKDMLRPYGESIQGRITIRDSQGISTMHDDYGVRFVSLTDVAGSGEKWESGASRAMNGSSEKAAGSGSRVFQGGSVEEVEDLVNLHMERAEELNETSLPSDGDTTAFNQSFYMLYLRRILSALPLSPHETFSHPVACVIAISSRNANPIDSLRNLYNSGNHLPLPDYLSTDYLRYYVLVHDEDRDDIKKSTLLFDQMKKHFGLHCHLLRLRSGGASVISDDDAVVVPKSQWISAAEELHEIRSRDYDDEQPTTCLPDSDATGIQTMVREMTQVSVIPFMERCIATWNDQVASRRRGISGRFLSMSKRYFSASSSRNSTSSSSNYDALNQSYPPSSPEAQMRKLADYAFMVRDWRLAHSIYELLRTDFNNDKAWKYHAGAQEMAAISLILTGTGLTSKVRADTIAPMLDLSCYSYLSRCSATYHALRCLLVAVELLRIRGGGAADEAATWAIRARDMNAQGTIGHALITERVGACYTIREGIGSGAWGARKRKAAMWKMLAAGEWLEVAKLAQSRRCLDTALPMYESSRFLHVHGFMEHLKQQAGYTPLLVDLQDGRGRRGREKGEEDDSDEEEGVEIQSETLHTGLKRKSLIVGREVDREVENEAGSGLDRFVDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8E6</accession>
    <name>D5G8E6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00004783001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430042">
        <property type="protein sequence ID" value="CAZ80789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836598.1">
        <property type="nucleotide sequence ID" value="XM_002836552.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8E6"/>
    <dbReference type="FunCoup" id="D5G8E6">
        <property type="interactions" value="489"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G8E6"/>
    <dbReference type="GeneID" id="9186009"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004783001"/>
    <dbReference type="eggNOG" id="KOG1018">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025810_8_2_1"/>
    <dbReference type="InParanoid" id="D5G8E6"/>
    <dbReference type="OMA" id="PCQMCAG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052717">
        <property type="term" value="F:tRNA-specific adenosine-34 deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002100">
        <property type="term" value="P:tRNA wobble adenosine to inosine editing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01285">
        <property type="entry name" value="nucleoside_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016192">
        <property type="entry name" value="APOBEC/CMP_deaminase_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11079">
        <property type="entry name" value="CYTOSINE DEAMINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11079:SF149">
        <property type="entry name" value="TRNA-SPECIFIC ADENOSINE DEAMINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00903">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="4">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ80789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="179" mass="18886" checksum="C4CD0B6B0CAA93D7" modified="2010-06-15" version="1">MQSALQIATSALQSNEVPVGCVFVHGSRIIASGRNDTNRSLCGTRHAELVAIDKILATHPPSIFKETDLYVTVEPCIMCASALRQIGIRKVYFGCSNDRFGGCGGVLRVHEGDGMGDGRNGGNGGGGGGYEVVGGIYSSSAVVKLREFYVQENGRAPVATSKKGRELKTDNLPVDVDVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8J8</accession>
    <name>D5G8J8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00002873001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430046">
        <property type="protein sequence ID" value="CAZ80820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836629.1">
        <property type="nucleotide sequence ID" value="XM_002836583.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8J8"/>
    <dbReference type="FunCoup" id="D5G8J8">
        <property type="interactions" value="68"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G8J8"/>
    <dbReference type="GeneID" id="9186040"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002873001"/>
    <dbReference type="eggNOG" id="ENOG502R9EV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025206_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8J8"/>
    <dbReference type="OMA" id="TKQMNAE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ80820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="542" mass="60814" checksum="8F5DE5FA2F2B69C6" modified="2010-06-15" version="1">MPEVATHTFKEYHNDICQSSDCTIALPPTYQRIHRAFSSPALVPARTIGRMGIHYEDSMIFEKGRAPIELLPVEIFDQIIPQLVTDVPTNGYSPRNKDLASCLLVSRTFHIATLSTLYSKVAFPHSFIFEKFLAQISKYPELGHLVKRLDFSHFTSVGFGRTERMNSEIQKLTATKLRNCLDITPNLREFLASEAMGRDVDERVLEKLFCELPRLNAVDFCATAASCFKDGFLSVISPSNLRLPIALPIRRLGMHGCNTLPSSVYATLLPRLLNLTHLDLTHTQVTDETLHAIPHTAKLTHLSLSRCNRLTGPAVVDFLINHPATRDLVYLNLLFDTGRYRLLSTTDVDVLLPFLPTTLRSLNLSGAKITRDHIPDLRRLSKHLEELSIGYADLNIADLNTIVKQDGPRHGGAKSNLHYLDLTGISSVNPTALVDTNRCKLLMPASFPLQVLELSEKVTDGLKERPISTKKLGWNVKSEHRRAWYLRAGPGTTPGSEKIAKPLAEETGFRYWKMGGKLWGGRKIGVGYGKVTGMYEYYGYRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8K0</accession>
    <name>D5G8K0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00002979001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430047">
        <property type="protein sequence ID" value="CAZ80843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836652.1">
        <property type="nucleotide sequence ID" value="XM_002836606.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8K0"/>
    <dbReference type="GeneID" id="9186063"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002979001"/>
    <dbReference type="eggNOG" id="KOG1558">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027089_1_2_1"/>
    <dbReference type="InParanoid" id="D5G8K0"/>
    <dbReference type="OMA" id="VFSPARH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040:SF55">
        <property type="entry name" value="MEMBRANE ZINC ION TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G00470)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040">
        <property type="entry name" value="ZINC/IRON TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="47"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="124"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="280"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="329"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="360"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="401"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="183"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="187"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ80843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="423" mass="45348" checksum="65544B418CF686CA" modified="2010-06-15" version="1">MNCPTRDPEPTGNPFFNQNPPALDKTLTTRSSLVCGGGSLSDDEYNLGLHIMALFLVLAQSSFACAFPLIAKKFPRLRIPPSFLFGAHHFGTGVLIATAFVHLLPTAFISLTDQCLPGFWNSTYPAMAGAIAMVAVFFVTIVEMVFTKGLCKGGCSDTNQRDVRCEAGDSYCNARDADEEYGGADKTGQKRCDGERLGTPGSEEIGGKGGVGRMGFGMAGKRRSRSHSVGQRLQKYEEMEKKGRQDQTLPGVAQLTPEQIHKKALLQCVLLEMGILFHSVFIGMALSVTIGPGFVILLIAIIFHQTFEGLALGSRIAVLNWKADAVQPWLMAVAYGLTTPVGQAIGLATHTLYSPSSQTGLLMVGIMNAISSGLLVFAGLVELLAEDFLSDESWLVLTGRKRIIACIYVMAGAFGMAFVGAFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8N1</accession>
    <name>D5G8N1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00003027001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430048">
        <property type="protein sequence ID" value="CAZ80874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836683.1">
        <property type="nucleotide sequence ID" value="XM_002836637.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8N1"/>
    <dbReference type="STRING" id="656061.D5G8N1"/>
    <dbReference type="GeneID" id="9186242"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003027001"/>
    <dbReference type="eggNOG" id="ENOG502T0E4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_564046_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8N1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="195"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="218"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="484" mass="53753" checksum="1E8B8E32BE30B351" modified="2010-06-15" version="1">MSTTNSPSKILDPPSTTPRKRQSLQHIMDIDRVRPARRRPGVLTEIENDGVGKKGEKGKPDFEVDRDKGWGRGSPTSLARFHVGLENEKWVGGGIQDGPEEEDEEGETTDTVPHRAPPKPSISELLLAHSRPKPDAVPLPTPPLSLLNRRHLQPTSAPALAIVSEPVMPKLKVESGPSPLQQMRLLRAQFEATRPAPPPPPPPPGPPPKQASRPSGVSAHPRASRPPSSAFAQKYTFTLHHLPQLTHHSKSAIISVHPPTHIHLQLRNLQELRGGGQYSYTIPSSSLPITVRNTMTSNQQTFEIEDLPLKHLHAVRYARHFVDALKKRTVVAKVEVRSGGVWTGRMFGDGKFEVIDPTGKIAGGVVGSRVKGPRRVVRMYEACQKILQDSDDLGRGEKSNNEKLEMGEFRIDERTDFVKGVGWCRRNEDGKGWRMMFLDGVRLEIEGASGDVLWIEGDQRKVLKRGMEVGDVRERVRMFVEKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8X7</accession>
    <name>D5G8X7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00004888001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430053">
        <property type="protein sequence ID" value="CAZ80970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836779.1">
        <property type="nucleotide sequence ID" value="XM_002836733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8X7"/>
    <dbReference type="GeneID" id="9181253"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004888001"/>
    <dbReference type="HOGENOM" id="CLU_2074856_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8X7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ80970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="118" mass="13199" checksum="EF9BB839E2C45EA2" modified="2010-06-15" version="1">MFLFFSKDHDFMLDQARPYASVKPITGICSSGISKVSEDKGEEQRDHTTIRRTNIMCTTTEYRSIATPMGFRPDFGHTKPSALATAYKAFLCIRISETISALPPKLLPDTRIVLAVID</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>D5G900</accession>
    <name>D5G900_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00004917001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430053">
        <property type="protein sequence ID" value="CAZ80993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836802.1">
        <property type="nucleotide sequence ID" value="XM_002836756.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G900"/>
    <dbReference type="STRING" id="656061.D5G900"/>
    <dbReference type="GeneID" id="9181276"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004917001"/>
    <dbReference type="HOGENOM" id="CLU_489337_0_0_1"/>
    <dbReference type="InParanoid" id="D5G900"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="305"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="82"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="172"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="312"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="557" mass="60352" checksum="0DF6BDCBE6D12F66" modified="2010-06-15" version="1">MAASSSSGIVERQYNYGGLIVRTFVHSAKSSPPPALGAEDSQPSPIGGSLPNSQVTSPVKRGRGRPRKNPKPNSVADDDAASDQSSQRASSSQQKPPKKKRRQQAGVVSGNSRIPAYVSLLTEEEMTAVRKRNRRGKQWIPGEKTVMNELARLGRSVPHPTETQQLQNGEDGVGDGEEPEPEFDLAEEIEPADEDESDDDVANILNCNPFMSALNDMPLDSTDNVEVGSRSASDWMRTDNEQSHFGQQTDSNGMTSFEYYMGVPNGASILEPPSAALDKDSSFAEQLNSTAPELSESILQQPDSMTTLSHLTPPPSSTSPQNPPPKRPSAPPPPAIHVDTTAALGAKLAELLNASKPAFIPTPFRCSFTLPLPEGEFTQKQYATDLVSAISDVDRYVYVTKNSTWNSTNSGSRGAIFVCNMSLESLKKRKSGTGEDQKKGMFSCPQLEIIIIFLFTVKSQLTRFLAVSMARTRYPCEGCITVRFLARKNVISVIYKHHAIHRSPHDKIEDSETALENNTATTTVSSAQDALFPFEKSAAEEITFPALAQAPSIAGPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>D5G956</accession>
    <name>D5G956_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">26S proteasome regulatory subunit RPN1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00003152001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 5">Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the proteasome subunit S2 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430055">
        <property type="protein sequence ID" value="CAZ81049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836858.1">
        <property type="nucleotide sequence ID" value="XM_002836812.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G956"/>
    <dbReference type="FunCoup" id="D5G956">
        <property type="interactions" value="1439"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G956"/>
    <dbReference type="GeneID" id="9184335"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003152001"/>
    <dbReference type="eggNOG" id="KOG2005">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008705_1_0_1"/>
    <dbReference type="InParanoid" id="D5G956"/>
    <dbReference type="OMA" id="GTCNGDI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905754">
        <property type="term" value="C:ascospore-type prospore nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034515">
        <property type="term" value="C:proteasome storage granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042176">
        <property type="term" value="P:regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000026">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016643">
        <property type="entry name" value="26S_Psome_Rpn1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002015">
        <property type="entry name" value="Proteasome/cyclosome_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041433">
        <property type="entry name" value="RPN1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040892">
        <property type="entry name" value="RPN1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10943">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10943:SF1">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01851">
        <property type="entry name" value="PC_rep"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18051">
        <property type="entry name" value="RPN1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17781">
        <property type="entry name" value="RPN1_RPN2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015965">
        <property type="entry name" value="26S_Psome_Rpn1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0647">Proteasome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="RPN1 N-terminal" evidence="7">
        <location>
            <begin position="65"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="26S proteasome non-ATPase regulatory subunit RPN1 C-terminal" evidence="8">
        <location>
            <begin position="848"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="49"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00057191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR015965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ81049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="902" mass="99699" checksum="8249E7FE8DF7B750" modified="2010-06-15" version="1">MAKENEKSTKLDKGKGKAADTPEKKDGDAFKEAQEKNGNGVKKGKKDELQEEELSEEDQQLKSELEMLVERLKEPNAELYKPALEAIKTFIRTSTSSMTAVPKPLKFLRPHYKSLTELYEQWPEGENKVAMADVLSVLGMTYADENRLDSLKYRLLAPMEDPGSWGHEYVRHLALEIGAEFTSRQEREEDTQDLTGLTMSLIPFFLKHNAEADAVDLLLELEIIDKLPPFLDENTYQRVGLYMISMVPLLSPPDDKTTLETAHSIYRKYNKLAQAITLAIRLNDVDLIREDLDAAEDPVLKRQLAFLVARQQISLDTPDQDINEALNNTRLSEHFAALGKELNILDPKTPEDIYKSHLENSRGSGGSVDSAKQNLANSFVNSFVNAGFGTDKLMLVEDESNSWVYKNKDLGMLSATASIGMLYQWNIDGLDVIDKYMDQEQEEENIKAGAALAIGILNCGVRSETDHAMALLYNDDYLENKSVPIRVSAIMGLGLAYAGSQRADFLEQLLPIIGDSSITLQVSAMAALSLGLIFVGSSNGDISSAIIQTLMERAEGDDPSLKDKWGRFLALGLALLFVGRQDDADVTLETLKAIEHPTSKHGGVLVDACAYAGTGNVLKYQSMLHLCNEHIDTSEDEKKGEDLFQSYAVLGLALIAMGEDVGAEMALRQFGHLMHYGDGIIRKTVPLAMGLLSASNPQIKVYDTLSRYSHDNDIDVAINAIFAMGLVGAGTNNARLAQLLRQLASYYHRDPNSLFMVRIAQGLLHMGKGTLSLNPFHNDRSVLSRVATAGLLTVLVSLIDAKSFILESSHYLLYYLVTAMHPRFLITLDENLQPLPVNVRVGQAVDVVGQAGRPKTITGWQTQNTPVLLAYGERAELEDDEYISLSNNLEGLVILKKVSNNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9I5</accession>
    <name>D5G9I5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">alpha-glucosidase</fullName>
            <ecNumber evidence="5">3.2.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Glucosidase II subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">GSTUM_00003340001</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing (1-&gt;4)-linked alpha-D-glucose residues with release of alpha-D-glucose.</text>
            <dbReference type="EC" id="3.2.1.20"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Glycan metabolism; N-glycan metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the glycosyl hydrolase 31 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.20" evidence="5"/>
    <dbReference type="EMBL" id="FN430062">
        <property type="protein sequence ID" value="CAZ81178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836987.1">
        <property type="nucleotide sequence ID" value="XM_002836941.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9I5"/>
    <dbReference type="FunCoup" id="D5G9I5">
        <property type="interactions" value="904"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G9I5"/>
    <dbReference type="GeneID" id="9184251"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003340001"/>
    <dbReference type="eggNOG" id="KOG1066">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000631_7_0_1"/>
    <dbReference type="InParanoid" id="D5G9I5"/>
    <dbReference type="OMA" id="TVHQPLW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017177">
        <property type="term" value="C:glucosidase II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004558">
        <property type="term" value="F:alpha-1,4-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106407">
        <property type="term" value="F:Glc2Man9GlcNAc2 oligosaccharide glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033919">
        <property type="term" value="F:glucan 1,3-alpha-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070880">
        <property type="term" value="P:fungal-type cell wall beta-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006491">
        <property type="term" value="P:N-glycan processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06603">
        <property type="entry name" value="GH31_GANC_GANAB_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14752">
        <property type="entry name" value="GH31_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000039">
        <property type="entry name" value="Glucosidase, alpha neutral C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000023">
        <property type="entry name" value="neutral alpha-glucosidase AB isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1760">
        <property type="entry name" value="glycosyl hydrolase (family 31)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048395">
        <property type="entry name" value="Glyco_hydro_31_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025887">
        <property type="entry name" value="Glyco_hydro_31_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000322">
        <property type="entry name" value="Glyco_hydro_31_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762">
        <property type="entry name" value="ALPHA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762:SF54">
        <property type="entry name" value="BCDNA.GH04962"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13802">
        <property type="entry name" value="Gal_mutarotas_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01055">
        <property type="entry name" value="Glyco_hydro_31_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21365">
        <property type="entry name" value="Glyco_hydro_31_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 12" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072015" description="alpha-glucosidase" evidence="13">
        <location>
            <begin position="23"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 31 N-terminal" evidence="15">
        <location>
            <begin position="87"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 31 TIM barrel" evidence="14">
        <location>
            <begin position="372"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 31 C-terminal" evidence="16">
        <location>
            <begin position="710"/>
            <end position="798"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF13802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF21365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAZ81178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="947" mass="107384" checksum="2358F0D9C4BDD7A7" modified="2010-06-15" version="1">MSARLTPILILSFLSLLQPAQMVKHENFKTCSQSGFCKRNRALADRAQSAGASWTAPYELDGKTLQLKDGVLTGKIWKTEAGGIELPLEVSLFENGVARVTIDEAQRRTGEITLRHDSKARKERYDGAEKWALVGDHKCQKTATMETKEGVTTVAYGPDNYEARITHKPFSVQFLKGGEVYIVFNDRNLINVEHWRPKIEKAEGEEPTEDEETYWEETFGGNTDSKPRGPESVALDITFPGYDHVYGIPEHATSLSLKETRGGEGNYNEPYRLYNSDVFEYETDSPMTLYGSIPFMQAQKKGSTVGVFWLSAAETWVDVIKTKQNPHALTTEKASTQTHWISESGLLDVFVLLGPDAAWVYRQYGELTGFTTLPAMFSIAYHQCRWNYVSQDDVKDVDRKFDKFDIPYDVIWLDIEHTDGKRYFTWDPLTFSKPESMQKTLARRDRKLVAIVDPHIKNDENYAVDKEMVAKGFGVKDKGGEKVYEGWCWPGSSHWVDCFNPAAVDWWKSLFKFDKFIGSTPNLWIWNDMNEPSVFNGPETTMPKDNIHHGNWEHRDVHNINGMSFHNATYEGLKIRLGSGKERRPFVLTRSFFAGSQRSAAMWTGDNQADWPHLQQAFPMLLANGIAGFPFAGADVGGFFGNPSKELLTRWYQAGAFYPFFRGHAHIDAKRREPYLAGEPYVSIIRDAIRLRYSLLPAWYTAFHQASREGMPILRPHFVMFPDDEAGFAIDDQFFLGGTGLLAKPIVKESATKTTVYFPDDQVYYDYFDHTTYHRKGHHEVSAALEEIPLFMRGGHIIPRKDRPRRSSGLMKYDPYTLVIAVSSEGTADGVLYVDDGETYAHQSGAYIHRAFTFSYGDNTLRSGNLHPEPAKAAAYNKDMRSVRVERIVIVGIGKEWAAKKVVEVKQGSKTWKAKIVYTEGKDGKANTAVVRDPKVSITKDWSVNFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9K9</accession>
    <name>D5G9K9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00003367001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430062">
        <property type="protein sequence ID" value="CAZ81202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837011.1">
        <property type="nucleotide sequence ID" value="XM_002836965.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9K9"/>
    <dbReference type="GeneID" id="9185339"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003367001"/>
    <dbReference type="HOGENOM" id="CLU_2814279_0_0_1"/>
    <dbReference type="InParanoid" id="D5G9K9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ81202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="67" mass="8135" checksum="E6115EE28DBCC473" modified="2010-06-15" version="1">MKLLEEVLVFRHVRRLRLQNDACAREYRHCCTSIVLYSYLVLAWWARLDKRSPLSTRVSYKYDTVLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9Z4</accession>
    <name>D5G9Z4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Argininosuccinate synthase</fullName>
            <ecNumber evidence="3">6.3.4.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Citrulline--aspartate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00003467001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the argininosuccinate synthase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.5" evidence="3"/>
    <dbReference type="EMBL" id="FN430068">
        <property type="protein sequence ID" value="CAZ81337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837146.1">
        <property type="nucleotide sequence ID" value="XM_002837100.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9Z4"/>
    <dbReference type="FunCoup" id="D5G9Z4">
        <property type="interactions" value="869"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G9Z4"/>
    <dbReference type="GeneID" id="9187035"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003467001"/>
    <dbReference type="eggNOG" id="KOG1706">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032784_4_2_1"/>
    <dbReference type="InParanoid" id="D5G9Z4"/>
    <dbReference type="OMA" id="WRWTVSP"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00113"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004055">
        <property type="term" value="F:argininosuccinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000053">
        <property type="term" value="P:argininosuccinate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000050">
        <property type="term" value="P:urea cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01999">
        <property type="entry name" value="ASS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000019">
        <property type="entry name" value="Argininosuccinate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1260.10:FF:000003">
        <property type="entry name" value="Argininosuccinate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1260.10">
        <property type="entry name" value="Argininosuccinate synthetase, chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00005">
        <property type="entry name" value="Arg_succ_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048268">
        <property type="entry name" value="Arginosuc_syn_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048267">
        <property type="entry name" value="Arginosuc_syn_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001518">
        <property type="entry name" value="Arginosuc_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018223">
        <property type="entry name" value="Arginosuc_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023434">
        <property type="entry name" value="Arginosuc_synth_type_1_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024074">
        <property type="entry name" value="AS_cat/multimer_dom_body"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00032">
        <property type="entry name" value="argG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001770">
        <property type="entry name" value="PRK00509.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11587">
        <property type="entry name" value="ARGININOSUCCINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11587:SF2">
        <property type="entry name" value="ARGININOSUCCINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20979">
        <property type="entry name" value="Arginosuc_syn_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00764">
        <property type="entry name" value="Arginosuc_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69864">
        <property type="entry name" value="Argininosuccinate synthetase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00564">
        <property type="entry name" value="ARGININOSUCCIN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00565">
        <property type="entry name" value="ARGININOSUCCIN_SYN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arginosuccinate synthase-like N-terminal" evidence="12">
        <location>
            <begin position="5"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Arginosuccinate synthase C-terminal" evidence="13">
        <location>
            <begin position="178"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF20979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ81337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="402" mass="44642" checksum="C3E3540B240BCC3A" modified="2010-06-15" version="1">MGKEKVCLAYSGGLDTSCILAWLIEKGYEVVCFMADIGQEEDFEAAKEKALKIGASSVHVVDLRKEFVEKLCYPAIQCNAIYENVYLLGTSLARPVIARGQIAVAEQEGCTLVSHGCTGKGNDQVRFELAFYALKPDVKVIAPWRLPEFYNRFAGRNDLLAYAAEVGVPVSQTKTKPWSMDENLAHCSYEAGILEDPDETAPKDLWKLTIDPLDAPDTPEDFTVHFEKGLPSKLEYEGGKVATEPLDLFLTANAIARRHGVGRIDIVENRFIGLKSRGCYETPGLTILRSAHVDLEGLVLDKEIRALRDQFVTFNYSKVLYNGLYFSPEREFLEDSITSSQKTVNGKVRCRVYKGSLIILGRSSETSKLYDMAESSMDEIGTSFAPVDTTGFISVQAIRLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>D5GA54</accession>
    <name>D5GA54_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00005137001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430075">
        <property type="protein sequence ID" value="CAZ81408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837217.1">
        <property type="nucleotide sequence ID" value="XM_002837171.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GA54"/>
    <dbReference type="FunCoup" id="D5GA54">
        <property type="interactions" value="1470"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GA54"/>
    <dbReference type="GeneID" id="9187106"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005137001"/>
    <dbReference type="eggNOG" id="KOG0266">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_57_23_1"/>
    <dbReference type="InParanoid" id="D5GA54"/>
    <dbReference type="OMA" id="LTPDANW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000111">
        <property type="entry name" value="Transcriptional repressor rco-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013890">
        <property type="entry name" value="Tscrpt_rep_Tup1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848:SF8">
        <property type="entry name" value="F-BOX AND WD REPEAT DOMAIN CONTAINING 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08581">
        <property type="entry name" value="Tup_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2 6" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Transcriptional repressor Tup1 N-terminal" evidence="9">
        <location>
            <begin position="15"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="309"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="344"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="385"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="430"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="484"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="526"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="83"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="164"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="13"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="196"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="221"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ81408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="569" mass="62217" checksum="9752DD0271BAB503" modified="2010-06-15" version="1">MYSSHRGMVPAPNSRLTELLEQVRQEFAEQQSRSSEYEHKIQQQIQEMDLVRQTVFQLEAAHGAMKQKYEDEIARLNRELDARGGAHVPPHGSQNAGPSAQPPPPSLPAGAGLFVARLSMVRNTSFPHHITPSSTTHLISPNYPSRSKIPISYNGLAPVLTFARAPQPTASPGPGKGRRPGGPGGPATPQQNQPPNYNSDPRSSPQVARPRSPASCLGDLEYDRVPPNQKKEGPDWFAIFNPKVSRLLDVDLVHTLEHNSVVCCVRFSQDGKYVATGCNRSAQIFDVKSGTMVSRLQDETVDRDGDLYIRSVCFSPDGRYLATGAEDKQIRVWDIQNRTIKQTFHGHEQDIYSLDFARNGRHIASGSGDRTVRVWDIESGQNVLTLSIEDGVTTVAISPDGRYVAAGSLDKSVRVWDAQTGYLVERLEGAEGHKDSVYSVAFAPNGRDLVSGSLDKTIKMWELSAARGLMPGGGTSRGKCVKTFEGHKDFVLSVALTPDGNWVLSGSKDRGVQFWDPRTASAQLMLQGHKNSVISVAPSPSGGLFATGSGDMRARIWSYEPYHSQSNPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5GA62</accession>
    <name>D5GA62_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00005150001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430075">
        <property type="protein sequence ID" value="CAZ81416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GA62"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005150001"/>
    <dbReference type="HOGENOM" id="CLU_2293751_0_0_1"/>
    <dbReference type="InParanoid" id="D5GA62"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ81416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="101" mass="11640" checksum="1694BD4E4809F798" modified="2010-06-15" version="1">MHEQPAQHSNTRCQEHIAAFLRYCDASAKATCSRRQLFSSLNSKSTLAREISTITQDPPRVMSLVSWAQVVRKIAWEDGNNMNLPRAAGRLPFNYYFYILY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAC0</accession>
    <name>D5GAC0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase</fullName>
            <ecNumber evidence="10">2.7.8.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00005227001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Functions in the biosynthesis of the anionic phospholipids phosphatidylglycerol and cardiolipin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>a CDP-1,2-diacyl-sn-glycerol + sn-glycerol 3-phosphate = a 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycero-3'-phosphate) + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12593"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:60110"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="2.7.8.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Phospholipid metabolism; phosphatidylglycerol biosynthesis; phosphatidylglycerol from CDP-diacylglycerol: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the CDP-alcohol phosphatidyltransferase class-II family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.5" evidence="10"/>
    <dbReference type="EMBL" id="FN430075">
        <property type="protein sequence ID" value="CAZ81474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837283.1">
        <property type="nucleotide sequence ID" value="XM_002837237.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAC0"/>
    <dbReference type="FunCoup" id="D5GAC0">
        <property type="interactions" value="396"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GAC0"/>
    <dbReference type="GeneID" id="9185756"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005227001"/>
    <dbReference type="eggNOG" id="KOG3964">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030471_1_1_1"/>
    <dbReference type="InParanoid" id="D5GAC0"/>
    <dbReference type="OMA" id="HKCLAQC"/>
    <dbReference type="UniPathway" id="UPA00084">
        <property type="reaction ID" value="UER00503"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008444">
        <property type="term" value="F:CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032049">
        <property type="term" value="P:cardiolipin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd09135">
        <property type="entry name" value="PLDc_PGS1_euk_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09137">
        <property type="entry name" value="PLDc_PGS1_euk_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016270">
        <property type="entry name" value="PGS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12586:SF1">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--GLYCEROL-3-PHOSPHATE 3-PHOSPHATIDYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12586">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--SERINE O-PHOSPHATIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000850">
        <property type="entry name" value="Phospholipase_D_PSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 10" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="8 10" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PLD phosphodiesterase" evidence="11">
        <location>
            <begin position="185"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ81474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="529" mass="58914" checksum="80332193AB88D792" modified="2010-06-15" version="1">MIVTTLRNSLPRPAVRLTRCRLCARSRRSFSSSNSALHPTAPISALTPPILGSLTGELDKLAPRFEIRANQICVIKTPSEFYSALKDRILKAERRIFLSTLYIGKTEHELIDILREALRRQPKLKVSILSDALRGTRETPNPSCASLLVPLISEFGADRVEIRMYHTPNLKGLKKKIVPQRLNEGWGLQHMKLYGIDDEIIMSGANLSTDYFTNRQDRYHIFSCPNLTGYFAGIHHAMSSISYNVLPGPKEQGGYALEWPESNPAPIPTEEPKEFQIAATSLLAPLIRPKLTPTKSPSSTPKTVVYPLAQFTPLLYPQDTSTEHPALSSVLRLLSSDAFADSRWVFTAGYFNIHTQLKKLLLASKSVEGIVVTAAPEANGFYGSKGVSGMLPAAYTLMARRFLDDVIKVGKGDKILLKEWRRGTIGKDPDAWTYHAKGVWISLPNEDDPSITLIGSSNYTKRSYSLDLEIDALVVTEDEGLKKALGEEVAWLQDNARTVTMQDFERTERRVSLKVKLALWIVKAIGGQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAE0</accession>
    <name>D5GAE0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00005117001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430075">
        <property type="protein sequence ID" value="CAZ81397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837206.1">
        <property type="nucleotide sequence ID" value="XM_002837160.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAE0"/>
    <dbReference type="GeneID" id="9187095"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005117001"/>
    <dbReference type="eggNOG" id="ENOG502S8DA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122021_3_0_1"/>
    <dbReference type="InParanoid" id="D5GAE0"/>
    <dbReference type="OMA" id="VTFTSYM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024512">
        <property type="entry name" value="Ser_palmitoyltrfase_ssu-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11779">
        <property type="entry name" value="SPT_ssu-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ81397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="84" mass="9774" checksum="807E6E0ADAC8E523" modified="2010-06-15" version="1">MSSSSLIQLLQRKRYQYEVTFSLYMLTPTEKIIFNSFLFVFLSMLILAASLYLPHHIHTIASRAWFYYAGDEELVHAPIPASMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAI2</accession>
    <name>D5GAI2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00003601001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430078">
        <property type="protein sequence ID" value="CAZ81525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837334.1">
        <property type="nucleotide sequence ID" value="XM_002837288.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAI2"/>
    <dbReference type="FunCoup" id="D5GAI2">
        <property type="interactions" value="178"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GAI2"/>
    <dbReference type="GeneID" id="9187465"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003601001"/>
    <dbReference type="eggNOG" id="KOG4549">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071162_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAI2"/>
    <dbReference type="OMA" id="GVWAWRK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010033">
        <property type="entry name" value="HAD_SF_ppase_IIIC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010036">
        <property type="entry name" value="MDP_1_eu_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01681">
        <property type="entry name" value="HAD-SF-IIIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01685">
        <property type="entry name" value="MDP-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17901:SF14">
        <property type="entry name" value="MAGNESIUM-DEPENDENT PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17901">
        <property type="entry name" value="MAGNESIUM-DEPENDENT PHOSPHATASE 1 MDP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12689">
        <property type="entry name" value="Acid_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01131">
        <property type="entry name" value="C1.5.2:_MDP_Like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ81525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="192" mass="21491" checksum="CC46D544719D3AED" modified="2010-06-15" version="1">MDPPKTLTDQHPLPKLIVFDLDYTLWPYWIDTHPTPPLSLTPSTTPPLTLHDATATPYPLFSTTLPLLTHLSTAAASGVKLAVASRSQAPELALQALGLYGLRQLFDFVEIYPGSKVRHMRRLREKSGVEFRDMLFFDDERRNRDVCELGVSFVLVGAAGVGCGLVDEGVRRWRRDRGFDGAREKEEGEEVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAR0</accession>
    <name>D5GAR0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00003747001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430085">
        <property type="protein sequence ID" value="CAZ81603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837412.1">
        <property type="nucleotide sequence ID" value="XM_002837366.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAR0"/>
    <dbReference type="GeneID" id="9187770"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003747001"/>
    <dbReference type="HOGENOM" id="CLU_866511_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAR0"/>
    <dbReference type="OMA" id="IGKYRRS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="170"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="254"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="54"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="171"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="255"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="268"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="321" mass="35128" checksum="D0A31729AB8649A6" modified="2010-06-15" version="1">MGLLDTLFNWVLTREQRERHSRAVWEKSVCRSSFPEVPATPYNTPTTPRSEAMTPSLSSSSTSSTSSAVSSGICSPVRSRPSTSYSTSPRPEYRRHTSITRSPISYDALIDTKSPTPAPPTKPTATAAPPLLYPLPPLGPMEGIGKYRRSIHVTEQFIDSLRRDNIAALQPLPSSTSSSPTNPAPYLTEDEPRQGRRLWRDCPGNRTTTTPPRAYSTSPTILPAEESPRRQRSLPPRWKAAAEIEVEEVVEPVRKGGRGVQSKRRVQGGRGKQQRVGGGKRQPAGPYPLPKAPRITGLGLKRVAPGRNMSASMETLLSGEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAY1</accession>
    <name>D5GAY1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosomal protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00005355001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430086">
        <property type="protein sequence ID" value="CAZ81674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837483.1">
        <property type="nucleotide sequence ID" value="XM_002837437.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAY1"/>
    <dbReference type="FunCoup" id="D5GAY1">
        <property type="interactions" value="255"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GAY1"/>
    <dbReference type="GeneID" id="9187842"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005355001"/>
    <dbReference type="eggNOG" id="KOG1569">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062853_1_0_1"/>
    <dbReference type="InParanoid" id="D5GAY1"/>
    <dbReference type="OMA" id="EFRVDKH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00403">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.790:FF:000001">
        <property type="entry name" value="50S ribosomal protein L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023674">
        <property type="entry name" value="Ribosomal_uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028364">
        <property type="entry name" value="Ribosomal_uL1/biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016095">
        <property type="entry name" value="Ribosomal_uL1_3-a/b-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023673">
        <property type="entry name" value="Ribosomal_uL1_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36427">
        <property type="entry name" value="54S RIBOSOMAL PROTEIN L1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36427:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL1M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00687">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56808">
        <property type="entry name" value="Ribosomal protein L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01199">
        <property type="entry name" value="RIBOSOMAL_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ81674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="223" mass="24523" checksum="60068FF28FDFCC8F" modified="2010-06-15" version="1">MQFSLTEAMRYIRAMEVGRDPISVKYDLAVRLRTPKSSPIIRNRIRLPTPVKTDKRVCVIAEGEQAEAARQAGAAIVGTDAVFERIRRGNLDFELCIAHEDSYGALLATKLAKVLGPKGLMPSVKFGTVVKNTGAAIRDLVGKSDYKERLAVVRLPIGQLRFTEAQLAENIKTFLSHLRRDIAALDNVTKTIHEVVLSSTHSSGFSLNGWIKPEATRGAPESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAZ9</accession>
    <name>D5GAZ9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00005377001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430086">
        <property type="protein sequence ID" value="CAZ81692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837501.1">
        <property type="nucleotide sequence ID" value="XM_002837455.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAZ9"/>
    <dbReference type="STRING" id="656061.D5GAZ9"/>
    <dbReference type="GeneID" id="9187860"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005377001"/>
    <dbReference type="eggNOG" id="ENOG502S0AG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033002_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAZ9"/>
    <dbReference type="OMA" id="CGIRKIW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037647">
        <property type="entry name" value="HIRIP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15410">
        <property type="entry name" value="HIRA-INTERACTING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15410:SF2">
        <property type="entry name" value="HIRA-INTERACTING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="384"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="266"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="276"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="389"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="425" mass="46857" checksum="BD5017D1A4A127CA" modified="2010-06-15" version="1">MDSDGDLSSIDSAPDARVITTALRNAVRKQFTEDPSRTTIKYIRARVENELSLGDDFLKRDSFWKAESKSIIEDEAGKQNALAEAAEPPIPQKEPTPEKQSPAKATKKPAKKGASRLKKLEDGLGDSTIEYPKKPAKKEKGVTSAKSTTRKAIAKPSILEDGDEDEDGDVQAKASREDTTKQASECEFSELDTSPKRAAKRTKSSTKPITLKKAKKTHEVAVEEENAENGEGKVSAEETPVVDEDPGNESEMSVVLDPAPLKKSRSQNPGAGSSKKQGKTKVTKSRPKERSKKGSSKADSNLSPDEEKIKTLQQWLLKCGIRKFWSKELAKFETPKEKIKHLTGLLTDVGMTPRYSNEKAKKIKEERELQAEVEAVQEGARWAKMDNGRVNTRSTRSAPSVSTVEKKQIRGMEDLAFLDDQSDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>D5GB78</accession>
    <name>D5GB78_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00003779001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430087">
        <property type="protein sequence ID" value="CAZ81771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837580.1">
        <property type="nucleotide sequence ID" value="XM_002837534.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GB78"/>
    <dbReference type="GeneID" id="9181481"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003779001"/>
    <dbReference type="eggNOG" id="ENOG502S1TZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048020_0_0_1"/>
    <dbReference type="InParanoid" id="D5GB78"/>
    <dbReference type="OMA" id="TTHEETY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="260"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="351" mass="39231" checksum="EC226BA214E215F2" modified="2010-06-15" version="1">MSFGHDSILSGGSSGSREGLAMNWVLDHVLAYPATYDFALPLRTIYILNSTTPHTSPADFKLLLLEHISNLPSQPCTLPPSFLSTFVRKCFPRDLENVQFDQAFTALDYIRDLEVRRSKELEKAARARGEGDRRIVNLRVKSIRTEQFYARAIAGIRRWTLLHELSITPFNKFNCIAILNTLYPIEEADINSYLPAPTLASQRHALWRYITGVEKNGPGILESVRTSNGGWAGVSEAVNAYLRLSLDMIQKADELARPSSIGSFRSDDSMDMGATSIAHTAPERKRKKGGFLRRDTISSETSEEEDGKASTLERIVRGLARLGSSQQHLGSPKKLYESDAGSEEDFPMRYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBG6</accession>
    <name>D5GBG6_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 15">Aurora kinase</fullName>
            <ecNumber evidence="1 15">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">GSTUM_00000464001</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1 15"/>
    <dbReference type="EMBL" id="FN430094">
        <property type="protein sequence ID" value="CAZ81859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837668.1">
        <property type="nucleotide sequence ID" value="XM_002837622.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBG6"/>
    <dbReference type="FunCoup" id="D5GBG6">
        <property type="interactions" value="942"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GBG6"/>
    <dbReference type="GeneID" id="9184433"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000464001"/>
    <dbReference type="eggNOG" id="KOG0580">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_63_6_1"/>
    <dbReference type="InParanoid" id="D5GBG6"/>
    <dbReference type="OMA" id="ESRFPEW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032133">
        <property type="term" value="C:chromosome passenger complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000939">
        <property type="term" value="C:inner kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990385">
        <property type="term" value="C:meiotic spindle midzone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990023">
        <property type="term" value="C:mitotic spindle midzone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140602">
        <property type="term" value="C:nucleolar peripheral inclusion body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035175">
        <property type="term" value="F:histone H3S10 kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051316">
        <property type="term" value="P:attachment of meiotic spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120110">
        <property type="term" value="P:interphase mitotic telomere clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033316">
        <property type="term" value="P:meiotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990758">
        <property type="term" value="P:mitotic sister chromatid biorientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010971">
        <property type="term" value="P:positive regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090267">
        <property type="term" value="P:positive regulation of mitotic cell cycle spindle assembly checkpoint"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903380">
        <property type="term" value="P:positive regulation of mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905824">
        <property type="term" value="P:positive regulation of mitotic sister chromatid arm separation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140429">
        <property type="term" value="P:positive regulation of mitotic sister chromatid biorientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902412">
        <property type="term" value="P:regulation of mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902115">
        <property type="term" value="P:regulation of organelle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904967">
        <property type="term" value="P:regulation of spindle attachment to meiosis I kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd14007">
        <property type="entry name" value="STKc_Aurora"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000235">
        <property type="entry name" value="Serine/threonine-protein kinase ark1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 11 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="122"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="70"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="77"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="245"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 13">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="200"/>
            <end position="202"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="249"/>
            <end position="250"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="12">
        <location>
            <position position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU367134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAZ81859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="395" mass="44435" checksum="89DC3C8532316F6E" modified="2010-06-15" version="1">MSLREIEARLAGISVNEENANPASGPHKSKTNKASSSTAGSLLQDKQSQQTGPPRHPLLKIAVHNQNHANNQWRPSEMPPPSPPPRKAQDASSEQQRKSDSSDALTIPRSMPSSREFHLGMFEIGRPLGKGKFGRVYLAKERKNGFVCALKVLHKSELQQGKVEKQLRREIEIQSNLRHPNILRLFGHFHDSKRVFLILEFAGQGELYKMLRKVGRFSERRAAEYIAQMAAALQYLHKKHVIHRDIKPENILVGIHGEIKISDFGWSVHAPNSRRTTMCGTLDYLPPEMLNGSNYHNEKVDLWSLGVLTYEFLVGAAPFEDSPVETQRRIVRADMKIPDFVSLEAADLIKKLLVVKPDQRIPLDEVAKHPWILKHCAPKPGANITKRSGGSTEKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBH0</accession>
    <name>D5GBH0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00000496001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430095">
        <property type="protein sequence ID" value="CAZ81863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837672.1">
        <property type="nucleotide sequence ID" value="XM_002837626.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBH0"/>
    <dbReference type="GeneID" id="9184437"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000496001"/>
    <dbReference type="eggNOG" id="ENOG502SMB6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1078451_0_0_1"/>
    <dbReference type="InParanoid" id="D5GBH0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072683" evidence="1">
        <location>
            <begin position="21"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="258" mass="27072" checksum="4F828148FBDCB0B8" modified="2010-06-15" version="1">MQFKSFSYLFLLTALPSALAQSFGTCTATNQLAGECIKTSTCSDQGGVSDPANLCPGDDTIQCCTYGTCKNGAGVAGTCQPTSTCKGTPDPGNHCPGPQNIQCCTGNGPNPPSDGGNLPGLDATQSRHARTIIAVAREYGVGNRGCQVSIVTAMQESRIRVLANPSVPDSNKYPHDGTGSDHDSVGIFQQRPQFWGTVKDCMDPKTSAGKFFTALKKVNGWERMEIGRAAQSVQRSAFPDAYTKHTPLAKGVCEAGGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBM7</accession>
    <name>D5GBM7_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Fimbrin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00005469001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ81877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837686.1">
        <property type="nucleotide sequence ID" value="XM_002837640.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBM7"/>
    <dbReference type="FunCoup" id="D5GBM7">
        <property type="interactions" value="556"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GBM7"/>
    <dbReference type="GeneID" id="9184451"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005469001"/>
    <dbReference type="eggNOG" id="KOG0046">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015284_3_0_1"/>
    <dbReference type="InParanoid" id="D5GBM7"/>
    <dbReference type="OMA" id="WQLMRKN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099079">
        <property type="term" value="C:actin body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032432">
        <property type="term" value="C:actin filament bundle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031097">
        <property type="term" value="C:medial cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120106">
        <property type="term" value="C:mitotic actomyosin contractile ring, distal actin filament layer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051666">
        <property type="term" value="P:actin cortical patch localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044396">
        <property type="term" value="P:actin cortical patch organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051639">
        <property type="term" value="P:actin filament network formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044837">
        <property type="term" value="P:actomyosin contractile ring organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070649">
        <property type="term" value="P:formin-nucleated actin cable assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd21294">
        <property type="entry name" value="CH_FIMB_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21297">
        <property type="entry name" value="CH_FIMB_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21300">
        <property type="entry name" value="CH_FIMB_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21303">
        <property type="entry name" value="CH_FIMB_rpt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000042">
        <property type="entry name" value="Fimbrin, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000010">
        <property type="entry name" value="Plastin-3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000016">
        <property type="entry name" value="Probable fimbrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000027">
        <property type="entry name" value="Probable fimbrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001589">
        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039959">
        <property type="entry name" value="Fimbrin/Plastin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19961:SF18">
        <property type="entry name" value="FI19014P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19961">
        <property type="entry name" value="FIMBRIN/PLASTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00019">
        <property type="entry name" value="ACTININ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00020">
        <property type="entry name" value="ACTININ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="7">
        <location>
            <begin position="131"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="7">
        <location>
            <begin position="280"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="7">
        <location>
            <begin position="404"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="7">
        <location>
            <begin position="529"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="92"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="105"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00073963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ81877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="640" mass="71413" checksum="E34CDE4B39AAD0AC" modified="2010-06-15" version="1">MNVFKLARKYPGLGQEGIMALQDTFLRSDQDEKGYLDEATAIKTAQDLERKPYDEVRATLKQVELDSSRRVELDDFVDLISKLRMTDGAKSSRAPVVSHARAGSIGRGGGSGGGQIKMGGATGATTHTINEDERTEFTRHINAVLLGDTDIGDRLPFPTDTFQMFDECKDGLVLAKLINDSVPDTIDERVLNRPNARSKKLNQFQMTENNNIVINSAKAIGCSVVNIGSGDIIEVREHLILGLIWQVIRRGLLGKIDIKLHPELYRLLEDDETLEQFLRLPPEQILLRWFNFHLKAANWHRRVANFSKDVSDGENYTVLLNQLAPDQCSRAPLQTRDLMQRAEQVLVNADKLGCRKFLTPKSLVAGNPKLNLAFVAHLFNTHPGLDPITEEEKLQVEDFDAEGEREARVFTLWLNSLDVQPAVNSLFDDLRDGTVLLQAYDKVIPGSVNWRHVNKAPSHGGELMRFKAVENTNYAVELGKQNRFSLVGIQGADITDGQRTLTLAITWQLMRRDITRTLQSLAQRVGVSEISDSYMLKWANDMARSGGKAQGIRSFKDPSLGSGVFLLDVLNGMKSNYVDYSLVTSGRTDEEAYLNAKLSISIARKMGATIWLVPEDIVAVRSRLIVTFLGSLMATYETLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBV8</accession>
    <name>D5GBV8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00005608001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ81958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837767.1">
        <property type="nucleotide sequence ID" value="XM_002837721.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBV8"/>
    <dbReference type="GeneID" id="9185468"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005608001"/>
    <dbReference type="HOGENOM" id="CLU_2777730_0_0_1"/>
    <dbReference type="InParanoid" id="D5GBV8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="69" mass="7477" checksum="3564CA787540182A" modified="2010-06-15" version="1">MMLKRKRFCIGSIVRGVVAEGETRRMRRGSRGSRGGGYCCARPPPVARSPPQESRSGAPISCLDSSCFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCC4</accession>
    <name>D5GCC4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00005815001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430109">
        <property type="protein sequence ID" value="CAZ82167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837976.1">
        <property type="nucleotide sequence ID" value="XM_002837930.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCC4"/>
    <dbReference type="STRING" id="656061.D5GCC4"/>
    <dbReference type="GeneID" id="9184844"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005815001"/>
    <dbReference type="eggNOG" id="ENOG502S0D7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008511_0_2_1"/>
    <dbReference type="InParanoid" id="D5GCC4"/>
    <dbReference type="OMA" id="WTIHGLA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000435">
        <property type="term" value="P:positive regulation of transcription from RNA polymerase II promoter by galactose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051127">
        <property type="entry name" value="Fungal_SecMet_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47424">
        <property type="entry name" value="REGULATORY PROTEIN GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47424:SF3">
        <property type="entry name" value="REGULATORY PROTEIN GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="608"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="8">
        <location>
            <begin position="56"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="135"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="261"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="696"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="32"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="135"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="173"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="261"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="710"/>
            <end position="722"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ82167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="794" mass="87896" checksum="535B467AA5D93267" modified="2010-06-15" version="1">MNQDKDSQDNSTDTGHHQLQHQIQFSPPPSQLGGGESNGGGGGSGSGSKRKRVSLACNACRTRKSKCDGVRPKCSMCRGKLITFICLFIGDLDFDCVYQQSASSSNTIIGKDYIGRLETRLLAVENLLAAQASESRLRNSPTLTAPNHDLNDDAQNRRSASADGPPSKRTRIEGSNTPNLDANNGETFVNLKLGQLEDESSAQDTVLDGMGALNLGDEEDYGYFGPSSNIAFIGHVTRALATSVSVTTTFTKEFRRTDPTLDSSSRFISPRNSPAPSTGGELDIRAGNVKIYVLPPQKRVLHYVTLYFQNTNILFPYLHQPSFLETYEEALRDGFSKVRRTWLALLNLVMAMGSRASTETDISAEEKYKTAEVFYQRGFGLCDGHFLRLASLDSVHLLLMMSHYLQSTRKPNQCWTTHGLAVRIALQLGLHSNQALQRFPPLERELRKRAWYGCVLLDRKLYTLLGMIIDTLYGGNLGGGDQVEPTFAYVARVAEIEEKLSTWRQQLPAALGIVSSSDIPSGGGPPSKFWRQSIILSLRYYNTRALLHRQFVVKGLSEIWGGGPSTNKFEGDFFWNFGWASIKVCAESAIETVEIIHKVKYRSDLLGFWWFTLYYTFNASLVLVSKVLIVGQVRQLALRPDFVPETASLLKHLEMAMETIEVLAAGNRIANRCRLFLKNLMRYVAPLATSSPRIVTSIPQPAQLPDRPSTDTPESSTVQNEPPQDPYQGSIEDLQKSPFRTQLGEFMLESDFTYLNSILPPLAMGDMSGMENGQSSSSGYVPNGSDYAHMSFGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCH8</accession>
    <name>D5GCH8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="12">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00005897001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase NEP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430109">
        <property type="protein sequence ID" value="CAZ82221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838030.1">
        <property type="nucleotide sequence ID" value="XM_002837984.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCH8"/>
    <dbReference type="FunCoup" id="D5GCH8">
        <property type="interactions" value="907"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GCH8"/>
    <dbReference type="GeneID" id="9186403"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005897001"/>
    <dbReference type="eggNOG" id="KOG3073">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055846_1_1_1"/>
    <dbReference type="InParanoid" id="D5GCH8"/>
    <dbReference type="OMA" id="VHNTFEL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005880">
        <property type="term" value="C:nuclear microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070037">
        <property type="term" value="F:rRNA (pseudouridine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18088">
        <property type="entry name" value="Nep1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1280.10:FF:000003">
        <property type="entry name" value="Ribosomal RNA small subunit methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005304">
        <property type="entry name" value="Rbsml_bgen_MeTrfase_EMG1/NEP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12636">
        <property type="entry name" value="NEP1/MRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12636:SF5">
        <property type="entry name" value="RIBOSOMAL RNA SMALL SUBUNIT METHYLTRANSFERASE NEP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03587">
        <property type="entry name" value="EMG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="8" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ82221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="250" mass="27391" checksum="BB8F5A6DED458087" modified="2010-06-15" version="1">MASPSSPVKKRPRTQSLPPLPSLTAEQAVPISAEDRDSKRLIVVLARACLETHKVSANGGEKYVLLNSDDHIGVLKKMGRDISDARPDILHQCLLTLLDSPVNKAGKLQVYIQSTKGVLIEVNPTVRIPRTFKRFAGLMVQLLHKLSIRSTSSSEKLLKVIKNPVSQYLPPNCRKVTLSWEAPVMNVREYISGLEDDESICVVVGAMAKGKDDFADGWVDEKIGVSNYSLSASVACSKFCHAAEDVWGIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCH9</accession>
    <name>D5GCH9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00005898001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430109">
        <property type="protein sequence ID" value="CAZ82222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838031.1">
        <property type="nucleotide sequence ID" value="XM_002837985.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCH9"/>
    <dbReference type="GeneID" id="9186404"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005898001"/>
    <dbReference type="eggNOG" id="ENOG502SK79">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_441459_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCH9"/>
    <dbReference type="OMA" id="FEMACCG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="611"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="35"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ82222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="645" mass="71038" checksum="F86D3909F36B5D6B" modified="2010-06-15" version="1">MILLPRPLSHLASPVGARKERGRDKYLVMDTSQLLNLPTEILVNILKFSASSTSPPPYKTLLNVALTCSALHHAVFCTENDLLWREPALALGIPESLRLDHVLYPGSSTLQKRLWRNVVKLNLAWGRPFPAKTPVIPKQQRAARQLPPVDKKGDWFGRRVVEVFVAGEGAKRENSYVTKPASVRDDGCAVFQVRPPGGPNGGIIPGLTYSLSGGPKVTCVLDPVMGKPREVILGNNRSHGWVASPLPLLTECVTPFPVQAGFVFEERSGEYRVRAVDASTAALSHSWNLGKRRPDRVVANGEILVAVTYAHPDSDGKTSQIPSNLVCVESFGGQEGRSRWKARTLAGSRIVWEFDLSIRWPQEMNRYTSFPHLKNFHITRTHLVCLVTQHPTATLGKLVGTDFYILDLETGGTVKRMKFSTKSPSTLPGSPRAAFTTALSHEFLLTDTYIVSGGAGGGLLVWNYVSDGHEPIYTIPDPLASDTTKPNGTASSLPRQCSALTMSGDGRYVAATTSDQLWLFDMVDKKVHAIYGNGRKIEARDYYARNPADDFPAGVWCWWKEWRSKRDQSGAMTWEEVDGSGGVAYLTGLDGEKRKLNVGKLLFDIAIGPHWAYLAFAMSFGMYSYVILMILWEKGLKGFEITGAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCS4</accession>
    <name>D5GCS4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00005997001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430120">
        <property type="protein sequence ID" value="CAZ82317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838126.1">
        <property type="nucleotide sequence ID" value="XM_002838080.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCS4"/>
    <dbReference type="GeneID" id="9187936"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005997001"/>
    <dbReference type="eggNOG" id="ENOG502R4JR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041041_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCS4"/>
    <dbReference type="OMA" id="WHVYNEL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.10">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050411">
        <property type="entry name" value="AlphaKG_dependent_hydroxylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042098">
        <property type="entry name" value="TauD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003819">
        <property type="entry name" value="TauD/TfdA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10696:SF54">
        <property type="entry name" value="FAMILY OXIDOREDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G13850)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10696">
        <property type="entry name" value="GAMMA-BUTYROBETAINE HYDROXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02668">
        <property type="entry name" value="TauD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TauD/TfdA-like" evidence="2">
        <location>
            <begin position="95"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ82317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="395" mass="44597" checksum="5F65FB7F2D27792E" modified="2010-06-15" version="1">MSTTALVETAKQPDITYHPDPISYAERTKRILAENPAQVLPAGFPSKLESELVWEGKDFKDGEWIYHLTESDVEEVRLAVAQFKSQKLPIGLLSPATFPLPSFSKELQRQARILHSGRGFFVLRGMPEYAGPLENTISYVGVASYIGPVRGIQDPNGAVLAHITDLRSQTDNTNAIGAPAYTSDKQVFHTDPGDIISLYVLGEAASGGTSRIASSWKVYNHLASTRPDIINTLSNAWPFDGFGGNPEVYYRPLIHFHNGRLISQYARRKFTGFRDLPRSTDIPPITEEMAEALDALHFTAERFSLGLHFRRGDIQFINNLSMFHSRDGYTDDETHKRHLLRIWSRNEELGWDTPDELKGLWEQLYYRGLKPEDQLFPLEPVVRRMVPIPGVEVSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>D5GD71</accession>
    <name>D5GD71_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00006075001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430142">
        <property type="protein sequence ID" value="CAZ82464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838273.1">
        <property type="nucleotide sequence ID" value="XM_002838227.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GD71"/>
    <dbReference type="GeneID" id="9183928"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006075001"/>
    <dbReference type="HOGENOM" id="CLU_2361264_0_0_1"/>
    <dbReference type="InParanoid" id="D5GD71"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ82464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="96" mass="11161" checksum="6E1F471D77D3A31A" modified="2010-06-15" version="1">MMGTVANFITTILLTLTCSAILFITYRHALRPLLVKRNIIPLRNLPRTFSDTFSAQGRVRLRDDDNRETRNRDLEEGFRDDSDDDDDDDEEASGWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDB3</accession>
    <name>D5GDB3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00006134001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430142">
        <property type="protein sequence ID" value="CAZ82506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838315.1">
        <property type="nucleotide sequence ID" value="XM_002838269.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDB3"/>
    <dbReference type="STRING" id="656061.D5GDB3"/>
    <dbReference type="GeneID" id="9183970"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006134001"/>
    <dbReference type="HOGENOM" id="CLU_1205537_0_0_1"/>
    <dbReference type="InParanoid" id="D5GDB3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="3">
        <location>
            <begin position="110"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="80"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="14"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="41"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ82506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="230" mass="24598" checksum="71BF3898BD2CBBC5" modified="2010-06-15" version="1">MPKLFLPPRKISEPSKNTSAASSTPVDPGTGSSLNISSSLPPTNVPVVNPNTRIPPAPPSNTGVVPPTLALPLSPLFADGNPHGKAITPPTLARPSPGQNRRKGPPRIVACVSCHRQKKKCAGERPCARCVALGVACKEQERPDDGFQYIRKKKKLKTAKTLDPLPQLPLHPLNHPTPFPGSLWTSMGQQASHDSPAYQNQLPSPSPSLTMIPVEMMNVVTEAHKRPVVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDQ2</accession>
    <name>D5GDQ2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Bis(5'-adenosyl)-triphosphatase</fullName>
            <ecNumber evidence="7">3.6.1.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00006223001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>P(1),P(3)-bis(5'-adenosyl) triphosphate + H2O = AMP + ADP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13893"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58529"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.1.29"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="3.6.1.29" evidence="7"/>
    <dbReference type="EMBL" id="FN430153">
        <property type="protein sequence ID" value="CAZ82645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838454.1">
        <property type="nucleotide sequence ID" value="XM_002838408.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDQ2"/>
    <dbReference type="FunCoup" id="D5GDQ2">
        <property type="interactions" value="154"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GDQ2"/>
    <dbReference type="GeneID" id="9181650"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006223001"/>
    <dbReference type="eggNOG" id="KOG3379">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056776_7_3_1"/>
    <dbReference type="InParanoid" id="D5GDQ2"/>
    <dbReference type="OMA" id="DAIYGMM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047710">
        <property type="term" value="F:bis(5'-adenosyl)-triphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004081">
        <property type="term" value="F:bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015964">
        <property type="term" value="P:diadenosine triphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01275">
        <property type="entry name" value="FHIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.428.10:FF:000011">
        <property type="entry name" value="Fragile histidine triad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051884">
        <property type="entry name" value="Bis(5'-adenosyl)-TPase_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039383">
        <property type="entry name" value="FHIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019808">
        <property type="entry name" value="Histidine_triad_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46243">
        <property type="entry name" value="BIS(5'-ADENOSYL)-TRIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46243:SF1">
        <property type="entry name" value="BIS(5'-ADENOSYL)-TRIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01230">
        <property type="entry name" value="HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00892">
        <property type="entry name" value="HIT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HIT" evidence="9">
        <location>
            <begin position="10"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Histidine triad motif" evidence="6">
        <location>
            <begin position="102"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="158"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-AMP-histidine intermediate" evidence="3">
        <location>
            <position position="104"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="97"/>
            <end position="100"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for induction of apoptosis" evidence="5">
        <location>
            <position position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR639383-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR639383-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR639383-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ82645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="191" mass="21159" checksum="868E022E2D43A310" modified="2010-06-15" version="1">MAASTQVLKRPVHFGSFLVTSQVFYSTAHSFALVNLKPLLPGHVLVCSNRVVPRLKDLSTDEVTDLFLTVQKVSKVIEKIYKADSLNIAMQDGVAAGQSVPHVHTHIIPRHFQDLPQEDQIYAMLESEDGDLGRNYLEAQHSAVMNNSARPKFPTVHPDAERKPRSEEAMAEEAKWLAGCMGEGEEGKGRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDR1</accession>
    <name>D5GDR1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00006232001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430153">
        <property type="protein sequence ID" value="CAZ82654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838463.1">
        <property type="nucleotide sequence ID" value="XM_002838417.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDR1"/>
    <dbReference type="FunCoup" id="D5GDR1">
        <property type="interactions" value="44"/>
    </dbReference>
    <dbReference type="GeneID" id="9181659"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006232001"/>
    <dbReference type="eggNOG" id="KOG4525">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009601_2_1_1"/>
    <dbReference type="InParanoid" id="D5GDR1"/>
    <dbReference type="OMA" id="MFRNNFG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.100.10.30">
        <property type="entry name" value="Jacalin-like lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001229">
        <property type="entry name" value="Jacalin-like_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036404">
        <property type="entry name" value="Jacalin-like_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053002">
        <property type="entry name" value="Metalloproteinase_M10B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021917">
        <property type="entry name" value="Unchr_Zn-peptidase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21054:SF2">
        <property type="entry name" value="MIP04191P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21054">
        <property type="entry name" value="ZINC METALLOPROTEINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12044">
        <property type="entry name" value="Metallopep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51101">
        <property type="entry name" value="Mannose-binding lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51752">
        <property type="entry name" value="JACALIN_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Jacalin-type lectin" evidence="1">
        <location>
            <begin position="423"/>
            <end position="572"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="572" mass="63043" checksum="DF45E8EE9493E38D" modified="2010-06-15" version="1">MNRLRFDFSASKIPPTSSGLAVNFLPLTSCPPLHLAILLAKDSPGEFECHGDLKLRGGNDVRMAKKKLRMAAYEITSPVKLVGFLISRRCFRLEEEWVADSLSSRDDGVMRSVAKVHILRTQKTVGGNNSWLYIQRREASRDVRGIWDIASEAVEDYFGEIAPVTKRHVAVLILDAKGDLRFGDLMNRSAFSNGMGLCGSRSLYALPSCLEEVVSAFSDCSRPGDAIENSDSSESGSSWEIATSVMKNMVEAFISPQPPTQPRDLQHINRTFLMKEPYSFRTKSPGLRFCVPKNESNWPRVDCLRFRYHPCFRIPTDPVPPALPDNPQVYAVESGIVAGAQSGIVLVEIYTDGVYRGHREFTNKPEREVLLLEHDLRWSLPDGHRTVPLKLKIYSAGQGKTVVEDFSTVKSSKVQLPNGYGVAFRSSSLGGPKAEGSRPQQLILHTVTPGRVLTSIRVFHNSALGGLEFMYEDSSTQLFGRRGSSESQEKVEVSDFPLDTRKGESLLGFCVRSGAQVEGIEILTSFGRRSPMFGNPHGGGASNTLIPPRGRSIAGVSGSYAQCVDGFSILYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDW3</accession>
    <name>D5GDW3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00006306001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prefoldin subunit beta family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430153">
        <property type="protein sequence ID" value="CAZ82706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838515.1">
        <property type="nucleotide sequence ID" value="XM_002838469.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDW3"/>
    <dbReference type="FunCoup" id="D5GDW3">
        <property type="interactions" value="785"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GDW3"/>
    <dbReference type="GeneID" id="9184501"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006306001"/>
    <dbReference type="eggNOG" id="KOG3478">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125172_2_0_1"/>
    <dbReference type="InParanoid" id="D5GDW3"/>
    <dbReference type="OMA" id="VQTEFAQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051131">
        <property type="term" value="P:chaperone-mediated protein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23161">
        <property type="entry name" value="Prefoldin_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.370:FF:000003">
        <property type="entry name" value="Prefoldin subunit 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002777">
        <property type="entry name" value="PFD_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21431">
        <property type="entry name" value="PREFOLDIN SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21431:SF0">
        <property type="entry name" value="PREFOLDIN SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01920">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="23"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ82706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="122" mass="13922" checksum="5ECB38752DCE11C3" modified="2010-06-15" version="1">MAGKMLQDLTDQFQALSKDMSSIVEARQKLDSQLQENRSVQKEFANLADDAKIYKLVGPVLVKQDKSEAVMNVDKRLEFINSEIARIEKQITDIQEKQERKKMEIIQVQSQLQQDAQAQVGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>D5GE50</accession>
    <name>D5GE50_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00006380001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430164">
        <property type="protein sequence ID" value="CAZ82793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838602.1">
        <property type="nucleotide sequence ID" value="XM_002838556.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GE50"/>
    <dbReference type="FunCoup" id="D5GE50">
        <property type="interactions" value="922"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GE50"/>
    <dbReference type="GeneID" id="9183125"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006380001"/>
    <dbReference type="eggNOG" id="KOG0733">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_8_4_1"/>
    <dbReference type="InParanoid" id="D5GE50"/>
    <dbReference type="OMA" id="MESNSAM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990275">
        <property type="term" value="F:preribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19518">
        <property type="entry name" value="RecA-like_NVL_r1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19530">
        <property type="entry name" value="RecA-like_NVL_r2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000018">
        <property type="entry name" value="Cell division control 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000365">
        <property type="entry name" value="Ribosome biogenesis ATPase RIX7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015415">
        <property type="entry name" value="Spast_Vps4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF171">
        <property type="entry name" value="NUCLEAR VALOSIN-CONTAINING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09336">
        <property type="entry name" value="Vps4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="215"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="496"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="116"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="59"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="125"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ82793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="747" mass="82371" checksum="5B774740939B67DC" modified="2010-06-15" version="1">MAGNRQSLTKGLERKVLAIVRRYIDELSVAGEKNGPLRLSVSQVFQYVITDESVPRQKKQNLEKMIEKALDTIRDEEAEADSLDSDLSEFEELNEEGFMEPKESNTMNKRVVNMWSTSTSSTTTPKPPPPIPEIPPPIASNTVPESPAPRKRKGRQPVKDSESAKRQKQDDSREPPKNISLKDIGGLEDVINDLLELIAMPLTHPEVYLHTGVQPPRGVLLHGPPGCGKTMLANAVAGEIGLPFIAISAPSIVSGMSGESEKKLRELFEEAREKAPCLIFMDEIDAITPKRESAQREMERRIVAQMLTCMDDLTLEKTSGKPVMIIGATNRPDSLDPALRRAGRFDREICLNVPDEVGREKILRVLCQKLRLSGDFDFKRLAKTTPGFVGADLNALTAEAGAVAMRRIYETLIIPPATIDTLETKIQLDSLHITLPDFLTALPKIQPSSKREGFATVPDVTWADIGALESLRVEMQMAIVQPIKRPELFARVGVTAPAGVLLWGPPGCGKTLLAKAVANESRANFISIRGPELLNKYVGESERAVRQVFTRARASIPCVIFFDELDALVPRRNDSLSESSSRVVNTLLTELDGLNDRKGIYVIGATNRPDVIDPAMLRPGRLDKPLFVDLPNTGERVEILKIITKNTPLSNVDLGAIAVDNRCKNFSGADLAALVREAAVLALRHACFTDVAEVEEGKNADNLEVMVTMEDFEKAFVNIRPSVSEDDREQYQELATRFGWGKTVLAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5GE76</accession>
    <name>D5GE76_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00006412001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430164">
        <property type="protein sequence ID" value="CAZ82819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838628.1">
        <property type="nucleotide sequence ID" value="XM_002838582.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GE76"/>
    <dbReference type="FunCoup" id="D5GE76">
        <property type="interactions" value="292"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GE76"/>
    <dbReference type="GeneID" id="9185475"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006412001"/>
    <dbReference type="eggNOG" id="KOG2743">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017452_0_1_1"/>
    <dbReference type="InParanoid" id="D5GE76"/>
    <dbReference type="OMA" id="GHSHMDP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03112">
        <property type="entry name" value="CobW-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1220.10">
        <property type="entry name" value="CobW-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036627">
        <property type="entry name" value="CobW-likC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003495">
        <property type="entry name" value="CobW/HypB/UreG_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051316">
        <property type="entry name" value="Zinc-reg_GTPase_activator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13748">
        <property type="entry name" value="COBW-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13748:SF31">
        <property type="entry name" value="ZINC-REGULATED GTPASE METALLOPROTEIN ACTIVATOR 1A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02492">
        <property type="entry name" value="cobW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90002">
        <property type="entry name" value="Hypothetical protein YjiA, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobW/HypB/UreG nucleotide-binding" evidence="1">
        <location>
            <begin position="34"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="388" mass="42770" checksum="31DBC2217A5A0E18" modified="2010-06-15" version="1">MSSDHLDLDDEEPPALVSTETLLSLEDLQLSKAPITIVTGYLGAGKTTLLNYILTEQHSKKIAVILNEFGNSSDIEKSLTIQRDGQQVEEWLELANGCICCSVKDIGVAAIEKLMERRGKFDYILLETTGLADPGNIAPMFWLDEGLKSTIFLDGIITLVDAANIIRTLEDQQEFSKDDEAGVGMTTAHLQISHADVVVINKADLVTREEMEIVEQRIAGMNGLARIIVTERGKVDNLGEWLLDLKAYENVGELKIPIKTRLDETISTVSLELPELDGEMLDSLDAWIRSLLWEAEVPSPSKKAGDQVSGHSNEQPILDIHRLKGRAITKTGNVLLIQGVRETFEIFDAPDQNLTAEGEGKLVLIGRGLRDVDVKGSLKWFLSRGKAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5GE87</accession>
    <name>D5GE87_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="12">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00006428001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat SEC13 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430164">
        <property type="protein sequence ID" value="CAZ82830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838639.1">
        <property type="nucleotide sequence ID" value="XM_002838593.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GE87"/>
    <dbReference type="FunCoup" id="D5GE87">
        <property type="interactions" value="1112"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GE87"/>
    <dbReference type="GeneID" id="9185486"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006428001"/>
    <dbReference type="eggNOG" id="KOG2445">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032441_1_1_1"/>
    <dbReference type="InParanoid" id="D5GE87"/>
    <dbReference type="OMA" id="NAPTRRW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031080">
        <property type="term" value="C:nuclear pore outer ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035859">
        <property type="term" value="C:Seh1-associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000578">
        <property type="entry name" value="Nucleoporin seh1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037363">
        <property type="entry name" value="Sec13/Seh1_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11024">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN SEC13 / SEH1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11024:SF3">
        <property type="entry name" value="NUCLEOPORIN SEH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="9" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="4 11" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="13"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="58"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="278"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ82830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="336" mass="37586" checksum="DB68E26F287F43B1" modified="2010-06-15" version="1">MPNSAAQSYTTFSTGHDDLVHDVAYDYYGRRMATVSSDQKLKVFDLSDDGEWVLSESIRAHEASITRVIWGPPEHGQIIATCSYDRMVRIWEEQEMGSTLRWKRQFQMTSEKRTAIYDISFPPATASTASGTSTGLKIAFISTDGIIHIYECREPQDLTHWIPMEAIDTLPTPPMKEIEVSFCIDFCPSRWSGEQLVVGAMDKVQVYRVGHDTVRLRPVEELKGHRGLVRDVSWAAGMGRSYHLIATGCKDGHVRIFKLTASPGHLSEGWSVELIADFDDHHSDVWRVSWNATGTVLSSAGDDGTIRLWKAAFSGEFQCLSVIASQRSGYALAEVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFH5</accession>
    <name>D5GFH5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00006903001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430208">
        <property type="protein sequence ID" value="CAZ83268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839077.1">
        <property type="nucleotide sequence ID" value="XM_002839031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFH5"/>
    <dbReference type="GeneID" id="9188216"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006903001"/>
    <dbReference type="eggNOG" id="KOG0065">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_35_1_1"/>
    <dbReference type="InParanoid" id="D5GFH5"/>
    <dbReference type="OMA" id="GINMESP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03233">
        <property type="entry name" value="ABCG_PDR_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029481">
        <property type="entry name" value="ABC_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034001">
        <property type="entry name" value="ABCG_PDR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010929">
        <property type="entry name" value="PDR_CDR_ABC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19241">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14510">
        <property type="entry name" value="ABC_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06422">
        <property type="entry name" value="PDR_CDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="532"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="556"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="611"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="638"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="668"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="169"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="780"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="53"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="792"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="830"/>
            <end position="848"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ83268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="848" mass="94737" checksum="FF26914892950C00" modified="2010-06-15" version="1">MAEEDSKLKRFSGVQVTPSTSRRGSGNWWGDEPINIDEALFDFEELRRELSLATRQSSRPNSRPTSRILAASRRASRQIEDVEEDVELGDVANVHGFDLEEYLQRSNKDAYNSGIIPKRIGVSFRNLGVSGTGSGYAVVSTFLDEFLNLFGKSILERGWEYGSKAVGKIWDRSGGKGRDVKYIIQGFSGVVRPGEMLLVLGRPGSGTSTFLRALTNQKREFTRIEGDIHYSGVPFELAEGRYRGEILFNSEEDIHHPTLTVQQTLKFALSTKTPATRIPGLSRDAFVQQMLELFAKMFGMKHTLNTMVGNSFIRGVSGGERKRVSIIEALASRATVNAWDNSTRGLDSSTAVDYIHALRILSTLTHSTNIVTLYQAGEQIYREFDKVCVIDSGRQIYYGRGSSATKYFESLGFHRNPRSTTADFLTSVSDPNKRKIKPGWEDKTPLTPRELEAAFKSSTHWTALQEELTAYDEELRSGRDARDFRDAVKQDKSRRSGKGSPYVVSLPRQIWYLTKRDFQLRLQDKVAMSSRFFNTLVLALIIGSLFFGIDRTSEGTFEIGGTLFFNIVVIGWMQMYEAIDMTVGRSITSKHTTFAFYRPSALVLAKSLVDLPILMVQCSLYTVVLYFMAGLEVNAGKFFLNLLFVFACTVCLTAFNRAVGAFSRDLNAAIRTAFFGLHVMIFFTGYLQPEGNMKSWVFKWIYWAQPLTYCLEALMVNQLEGVFIRCSEDQLIPDIPSASVANKVCQLAGSEQGTEFISGTDFLQAAWGYSRSHVWRNFGINMESPEPPQGSTRNGRGTGTNRPIPHAPPSFRKPGRSPRRWKADSPKPPTATMTKPKAAAAPATPGGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFJ0</accession>
    <name>D5GFJ0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00006927001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430208">
        <property type="protein sequence ID" value="CAZ83283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839092.1">
        <property type="nucleotide sequence ID" value="XM_002839046.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFJ0"/>
    <dbReference type="STRING" id="656061.D5GFJ0"/>
    <dbReference type="GeneID" id="9188231"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006927001"/>
    <dbReference type="eggNOG" id="ENOG502SHSC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_338365_0_0_1"/>
    <dbReference type="InParanoid" id="D5GFJ0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1020">
        <property type="entry name" value="Prion-inhibition and propagation, HeLo domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029498">
        <property type="entry name" value="HeLo_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038305">
        <property type="entry name" value="HeLo_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37542">
        <property type="entry name" value="HELO DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14479">
        <property type="entry name" value="HeLo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prion-inhibition and propagation HeLo" evidence="2">
        <location>
            <begin position="10"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="237"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="281"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="253"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ83283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="841" mass="92555" checksum="A9A9DFED40941795" modified="2010-06-15" version="1">MDPVSFLSLVAGIPGLFTSCLQVYDLISSAASHGSDFQVMLSKVDVEHARFILWGGTVGLIQSSASSTGSAFDPRIVHPHILEPVAKILSCIQKSFEDTEKLKKRYGLVAGGGKSSSLPNDSIQLYERPTPAFLSLSIFKEIYERQQLAGEEMQKQNSVLKKARWAIVDKGRFSTAINDLKTFNDSLHRLLPDVEWKTQERLRQEVLASQDENVLRLLQQASEGKHTGLKRAATTRLNRLNSRHQPPQIAAPEAHELPAINDRDFSNPRIWDVTTSPGWASGQGSYVEADGVEKPRSKSFPNAATLAGPWDQGGHPNSPAELPSSGSPMDIRGSARSLGGRGPRAELEIFTSSRATEGEALDGPKSPDIVYLAGMHNLLDFPDYFSGEMTPPPNDAIPELSAETFNHVHVPPKSGYATSPPLTPNPTEAFYICIDFQDSGTRAAIAHSSTPKDSDVVGGWPDNDTCDSYIQSAWVPSVLAYDRSDGGCQWGYQVQESQDQAGWFIAHLQGEKTGLMTYLRRHDKALREALEEYFYHLLRHLLEAAEKKYGRDIYINSGIGCALIVSNKWENGVRTPLKDAVQNALLSLGINSDVALQVHTSLAASYISARLLTLHLEEKIGTGGNSVEGNGGLFMVCHISAEARKSITIQTLQGRPTPSPEATNYRPPQFSSVGMQFVFREFEKLLERHYEYTGIRSHKLYDSVRKNWLRAVQEFSGRQNSVWISCPHYPNCYECHCVGGELSITKGQMEGILNIFFAEVCAQVREGLKKVPGQVGGSRAFKGIYLCGDLGSECSAMYGYLRRELPEQSIYQLPRSATEVATGATVAALKHRSNLPIKAIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFV5</accession>
    <name>D5GFV5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00007105001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430220">
        <property type="protein sequence ID" value="CAZ83398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839207.1">
        <property type="nucleotide sequence ID" value="XM_002839161.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFV5"/>
    <dbReference type="STRING" id="656061.D5GFV5"/>
    <dbReference type="GeneID" id="9181743"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007105001"/>
    <dbReference type="eggNOG" id="ENOG502S447">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_138059_0_0_1"/>
    <dbReference type="InParanoid" id="D5GFV5"/>
    <dbReference type="OMA" id="WLHVPME"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018788">
        <property type="entry name" value="Proteasome_assmbl_chp_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053720">
        <property type="entry name" value="Psm_Assembly_Chaperone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31051">
        <property type="entry name" value="PROTEASOME ASSEMBLY CHAPERONE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31051:SF1">
        <property type="entry name" value="PROTEASOME ASSEMBLY CHAPERONE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ83398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="145" mass="15583" checksum="92B78436108D20C4" modified="2010-06-15" version="1">MESVLKSPFPASTKRATSVIDGISTESTVVYFADKIMITLSQDGRLAQWFHVPLDTNNSALIGAQSAEDEDGLLPLTHLTPTTLLGGAVESREALGQLIAVQLASAVSMRNRDEGRMVVLGLGLMKAELERDAFIQLIELVGKCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFX4</accession>
    <name>D5GFX4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00001923001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thiolase-like superfamily. HMG-CoA synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430222">
        <property type="protein sequence ID" value="CAZ83417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839226.1">
        <property type="nucleotide sequence ID" value="XM_002839180.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFX4"/>
    <dbReference type="STRING" id="656061.D5GFX4"/>
    <dbReference type="GeneID" id="9181761"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001923001"/>
    <dbReference type="eggNOG" id="KOG1393">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2089226_0_0_1"/>
    <dbReference type="InParanoid" id="D5GFX4"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004421">
        <property type="term" value="F:hydroxymethylglutaryl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006084">
        <property type="term" value="P:acetyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010142">
        <property type="term" value="P:farnesyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00827">
        <property type="entry name" value="init_cond_enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000590">
        <property type="entry name" value="HMG_CoA_synt_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013746">
        <property type="entry name" value="HMG_CoA_synt_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013528">
        <property type="entry name" value="HMG_CoA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43323">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL COENZYME A SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43323:SF2">
        <property type="entry name" value="HYDROXYMETHYLGLUTARYL-COA SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08540">
        <property type="entry name" value="HMG_CoA_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01154">
        <property type="entry name" value="HMG_CoA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01226">
        <property type="entry name" value="HMG_COA_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Hydroxymethylglutaryl-coenzyme A synthase N-terminal" evidence="3">
        <location>
            <begin position="5"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Hydroxymethylglutaryl-coenzyme A synthase C-terminal" evidence="4">
        <location>
            <begin position="77"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ83417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="131" mass="13958" checksum="D98E5735502822A4" modified="2010-06-15" version="1">MQILSPNTNIEGVDTVNACYGGTNALFNAINWVESSAWDGRNAIVVAGDIALYSKGNARPTGGAGCIAMLVGPDAPLVLEPGKRGSYMSHAYDFYKPDFTSEYPVVDGYYSIRCYTEAVDGCYKAVPTAVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GG84</accession>
    <name>D5GG84_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00001998001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430246">
        <property type="protein sequence ID" value="CAZ83527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839336.1">
        <property type="nucleotide sequence ID" value="XM_002839290.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GG84"/>
    <dbReference type="GeneID" id="9184588"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001998001"/>
    <dbReference type="HOGENOM" id="CLU_2741888_0_0_1"/>
    <dbReference type="InParanoid" id="D5GG84"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ83527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="71" mass="8246" checksum="CC2B71C489E757AD" modified="2010-06-15" version="1">MVEMVEQLLLQIPRYTEVVNIKDWAPVSHLPRQAFGICIFTCLTFPSYTYPDVPIIDSPSFMLLERRSTLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGA9</accession>
    <name>D5GGA9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007302001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430253">
        <property type="protein sequence ID" value="CAZ83552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839361.1">
        <property type="nucleotide sequence ID" value="XM_002839315.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGA9"/>
    <dbReference type="GeneID" id="9183150"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007302001"/>
    <dbReference type="HOGENOM" id="CLU_2814287_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGA9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="67" mass="7688" checksum="B8A057B4672DF832" modified="2010-06-15" version="1">MESLVMSPLFLLFDGTDFCFLLGGGVIDFVYFFLLLIIIIAGVYPILFSCIRQLMYVYGWSMWEGSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGB3</accession>
    <name>D5GGB3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00007307001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430253">
        <property type="protein sequence ID" value="CAZ83556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839365.1">
        <property type="nucleotide sequence ID" value="XM_002839319.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGB3"/>
    <dbReference type="STRING" id="656061.D5GGB3"/>
    <dbReference type="GeneID" id="9183154"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007307001"/>
    <dbReference type="eggNOG" id="ENOG502RX7Y">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011522_1_0_1"/>
    <dbReference type="InParanoid" id="D5GGB3"/>
    <dbReference type="OMA" id="PFNMTLT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF12">
        <property type="entry name" value="ZN(2)-C6 FUNGAL-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="3">
        <location>
            <begin position="19"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ83556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="626" mass="70534" checksum="372CA8904467A208" modified="2010-06-15" version="1">MQPKAPKTGNKTHRRSRTGCFTCRLRRKKCDEGKPLCKACKHLGLSCEYKRPIWWGNVDQRKRQKENIKMLIKRTKLSEKVAQISPINTIARRRSLSMESGLSLEEEMENYNELVNPEIHHGHIHHPYHPFPPFEVDIMTEKQTYVNSFPMPMRRASTSTASYTSSHHMRRDSAANVMVPTDGLAQFEAMNGHVHHHPASCTSTEESLPATADFGLFDYGYGQPEAPMPYIQVDESDRHLLNHFVENVLPIIFPILDLNLDAKRDVILPALASNECYRHCCLSIAALHLKVTQGLQSEWIDQSITRHRYATISGLCDALARDQDHLQILEATLGMVLFQCSVGSPDDNLPDIPWHQHFQAVTSLVNKMNLTQYLQHISQNNHSPPSVNMTLTAWIDILGATMLGRAPQFADTYREKHLSNGISGLRELMGCDDKVMYLISEIACLEALKNDGMLSDIDLCDHVKSLAHQIDLTESSLDQPMPTSPIITANGQIDTRQLTNATTDAFRFAARIYLCSLVPGFDRHQPQVMELLNKLTHCLESIPAGPDGFDRSLVWVYLIGGSVATPGSLFMRFFKSRCSALGEAGACGSFGRMARILEILWGRRESGWEGGWRDVMEANSWDFLLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGP1</accession>
    <name>D5GGP1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="5">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00007445001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="5"/>
    <dbReference type="EMBL" id="FN430275">
        <property type="protein sequence ID" value="CAZ83663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839472.1">
        <property type="nucleotide sequence ID" value="XM_002839426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGP1"/>
    <dbReference type="FunCoup" id="D5GGP1">
        <property type="interactions" value="445"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GGP1"/>
    <dbReference type="MEROPS" id="C19.A61"/>
    <dbReference type="GeneID" id="9185585"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007445001"/>
    <dbReference type="eggNOG" id="KOG1867">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008279_11_2_1"/>
    <dbReference type="InParanoid" id="D5GGP1"/>
    <dbReference type="OMA" id="NVSCNCI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071819">
        <property type="term" value="C:DUBm complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046695">
        <property type="term" value="C:SLIK (SAGA-like) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02660">
        <property type="entry name" value="Peptidase_C19D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF937">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBP-type" evidence="7">
        <location>
            <begin position="39"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="6">
        <location>
            <begin position="187"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ83663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="524" mass="58551" checksum="14B7DF5D8DCA68FF" modified="2010-06-15" version="1">MGSTTILNRPQAASALSASPKVVKNNKSLASSSAAPLTYGCNHIEKPLQGPTEGNKMRVLYGSATRVALDSSRAHAQTCEKCKFLITRTFLCMQCPSIYCPKDAEAHYKEKNHRFGIDSRSGHVYCFPCQDFIYDPALEKIRVEKEVAMEGGARKRRRLDDIKPSPEDLKYIESNTTLAPCRATGLRGFLNMGSTCFMSVVLQSLIHNPLVRNFYLADGHKPKECAQANCMSCAMEEVFSEFFATDKTDGFGPVNLLTTSWKCEQTLAGYQQQDAHEYLQFLLNQLHATNGGNTDTKTEQCKCIIHRAFYGNLQSDVTCEGCQNVTTAVDPVMDLSLDLRKKDKRKLATPKTEGDGAMQTLQECLERFTSTEKLGMNEYNCENCSGHQEVATKQLTVKRLPPVLCIQLKRFEHGSTKSSKIDTKIRFPMQLDMAPYTTRAKRKAKDGSTDCGPKSSLKGHGPYLYDLLSVVVHTGQINSGHYINFSRENGQWFRFDDSVVTLATEKDVLSAKAYLLFYIIRSLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGR1</accession>
    <name>D5GGR1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007479001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430275">
        <property type="protein sequence ID" value="CAZ83683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839492.1">
        <property type="nucleotide sequence ID" value="XM_002839446.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGR1"/>
    <dbReference type="GeneID" id="9185605"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007479001"/>
    <dbReference type="eggNOG" id="ENOG502S65B">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096918_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGR1"/>
    <dbReference type="OMA" id="RDPIHEI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="12"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="136" mass="14343" checksum="0B46109764EEE57B" modified="2010-06-15" version="1">MSSAAIFIPNRPGDSSPSSSTSSEAPSPSASPASPPAGASGSPPPSRLPSSSSIYHRRRPSLLSSSVSRVEHSVFDLGIRDGSPRLITCVKASQGFDWNQDIFLPSSSEITIHNGIQKPETVHEIILSEDESFLPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGT0</accession>
    <name>D5GGT0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00007499001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the unc-50 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430275">
        <property type="protein sequence ID" value="CAZ83702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839511.1">
        <property type="nucleotide sequence ID" value="XM_002839465.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGT0"/>
    <dbReference type="FunCoup" id="D5GGT0">
        <property type="interactions" value="640"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GGT0"/>
    <dbReference type="GeneID" id="9181672"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007499001"/>
    <dbReference type="eggNOG" id="KOG3012">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066239_1_2_1"/>
    <dbReference type="InParanoid" id="D5GGT0"/>
    <dbReference type="OMA" id="YRNFMYR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007881">
        <property type="entry name" value="UNC-50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12841">
        <property type="entry name" value="PROTEIN UNC-50 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12841:SF6">
        <property type="entry name" value="PROTEIN UNC-50 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05216">
        <property type="entry name" value="UNC-50"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="93"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="176"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="214"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ83702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="249" mass="28436" checksum="043DB2F6C7628877" modified="2010-06-15" version="1">MYAPRTSPPPTRSTFAPHIAAPRHGGRSDSSSGSRLPLFFRRMIKFPQMDFEMAIWEMTYLIMAPKKVIRTIYYHKRKIPGLAMTPFASIMRLIFSLVFLHFLLTSVIFASTGYIMCGRFLKVPGTGGLGRAGVVAGEGEMEFMYCLEIGVRAFFPVWVFLYVIQFLMMPILTKDYWISIFLGNSLYLIAFSFYCVITFLGYNALPFLHHTEIMLFPIFVLVVLWAISLFGFNLTKHIVPILLPVTGRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>D5GI06</accession>
    <name>D5GI06_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00008218001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430322">
        <property type="protein sequence ID" value="CAZ84149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839958.1">
        <property type="nucleotide sequence ID" value="XM_002839912.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GI06"/>
    <dbReference type="STRING" id="656061.D5GI06"/>
    <dbReference type="GeneID" id="9184234"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008218001"/>
    <dbReference type="eggNOG" id="ENOG502QTSJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019363_1_0_1"/>
    <dbReference type="InParanoid" id="D5GI06"/>
    <dbReference type="OMA" id="CWPYVSM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012316549" evidence="2">
        <location>
            <begin position="16"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="468"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="473"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="501"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="511"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="523"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ84149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="566" mass="59301" checksum="0841A422BC7857CF" modified="2010-06-15" version="1">MSIFLWLLFLPAVLCQLSSGSLKKFPNALALGNNFDPIIAAYWTGLPHHRRTPFSVSPDGKSAYLAYLSTSYASVFIQQVDPATFAAVGKPVEISGAREAGGLVARNDGFAVLVNIDNPSTEHPISTIVRYKGGTKAWTRPVNGPGVHESDGLSSSPDMNGDLVYSEAAGLYAAYFVVTAYTGGASGHFGDSIQYVNDSGEIKNIAGASSTFGCSHNTGIALEEADAAPFASVCAEDHGAIWLNTKTQYMSGVKIANENTTNGVSGEPMGGMGGSYSSLALFPGTSTYIFAWQSRGAVSLTRDDWMGDGYTSCSPRWLNHNVAISMLKDKSTLQGPEAISKVGAASGDDQVTWITKSSTTDQQNVRVAALDSSNAIVTWEQLSNPHCDPVPLSCTGTFSGTFFQQVDSQGKKVGEAISAKDVLVAGDMQRIEGKGVCWPYVSMDWDLSQPKDRGTYVSSMSFACIGGSGSGTPAPTSQQSTATPSETAPPVAGPSETKTESSAASVPSPATTLVTPEATNMNTRSPATTPTSSAPYAFTTCDAKNGCPDGLRCYSFKSGYSVCIPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5GI41</accession>
    <name>D5GI41_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00008257001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430323">
        <property type="protein sequence ID" value="CAZ84184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839993.1">
        <property type="nucleotide sequence ID" value="XM_002839947.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GI41"/>
    <dbReference type="STRING" id="656061.D5GI41"/>
    <dbReference type="GeneID" id="9181902"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008257001"/>
    <dbReference type="eggNOG" id="KOG1102">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003663_0_0_1"/>
    <dbReference type="InParanoid" id="D5GI41"/>
    <dbReference type="OMA" id="WRRMATR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000055">
        <property type="entry name" value="TBC domain-containing protein C1778.09"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000023">
        <property type="entry name" value="TBC domain-containing protein C1778.09"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF9">
        <property type="entry name" value="GTPASE ACTIVATING PROTEIN AND CENTROSOME-ASSOCIATED, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="2">
        <location>
            <begin position="64"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="325"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="334"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ84184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="359" mass="39538" checksum="BCD888AAC27DD4D4" modified="2010-06-15" version="1">MLRSPLPGATATLHAKENSRSKKWREMAVVRKRGERGEEGGKGGGTEWGFVTVDAKLISRTWKGIPDCWRAAAWHAFLTASAKRRGIGRSDTELVTIYTDFIERPSADDVQIDLDVPRTISSHIMFRRRYRGGQRLLFRTLHALSLYFPEVGYVQGMASLAATLLCYYDEETTFIMLVRMFELRGLEKLYQAGFGGLMQALDEFEGSWMKGGQISRRLTSLGVTPTTYGTRWYLTLFNYSMPFAAQLRVWDVFMLLGDPDPASSAVTPAFSGSLDVLHATSAALIDGMREILLKSDFEGSMKVLTSWIPVKDEELLMKVVKGEWKGRRKRRSRGGGSGGGGAASGGGEGGGGASPVPQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>D5GI71</accession>
    <name>D5GI71_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00008312001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430323">
        <property type="protein sequence ID" value="CAZ84214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840023.1">
        <property type="nucleotide sequence ID" value="XM_002839977.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GI71"/>
    <dbReference type="STRING" id="656061.D5GI71"/>
    <dbReference type="GeneID" id="9181932"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008312001"/>
    <dbReference type="eggNOG" id="ENOG502S06V">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085110_0_0_1"/>
    <dbReference type="InParanoid" id="D5GI71"/>
    <dbReference type="OMA" id="FPKAKRD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054256">
        <property type="entry name" value="DUF6987"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39461">
        <property type="entry name" value="LEA DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_8G04920)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39461:SF1">
        <property type="entry name" value="LEA DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_8G04920)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22485">
        <property type="entry name" value="DUF6987"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="189"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="DUF6987" evidence="3">
        <location>
            <begin position="44"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ84214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="248" mass="26461" checksum="7AD6F49B2EAADEE4" modified="2010-06-15" version="1">MSTKVTQKNLQDVPPEPINLEEAAAPESKNTSANTAKEDTESNGEAKEEAAKIAKKMSGIIDPAIDRLKPILQMITEHVDKANNTKKEDLDEEKLVDQLKPLIEQATGVLQETHGAIKALDPDGKVARSAQYKASDSEASPEEQHLAQGLAELTGNVAKTIQNAKDKIKDMPHAKKNLGPLLDLLQTPLFQIISAVGLLLNGVLTLLGNLLDALGLGGIVRNVLGGLGLAKLLDGLGLGQWLKQPDSK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-07-27" modified="2025-06-18" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>D5GI81</accession>
    <name>PFF1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Vacuolar membrane protease</fullName>
            <ecNumber evidence="6">3.4.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">FXNA-related family protease 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ORF">GSTUM_00008325001</name>
    </gene>
    <organism>
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in vacuolar sorting and osmoregulation.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="2">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase M28 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.-.-" evidence="6"/>
    <dbReference type="EMBL" id="FN430324">
        <property type="protein sequence ID" value="CAZ84224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840033.1">
        <property type="nucleotide sequence ID" value="XM_002839987.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GI81"/>
    <dbReference type="SMR" id="D5GI81"/>
    <dbReference type="FunCoup" id="D5GI81">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GI81"/>
    <dbReference type="EnsemblFungi" id="CAZ84224">
        <property type="protein sequence ID" value="CAZ84224"/>
        <property type="gene ID" value="GSTUM_00008325001"/>
    </dbReference>
    <dbReference type="GeneID" id="9181942"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008325001"/>
    <dbReference type="eggNOG" id="KOG2194">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006412_1_0_1"/>
    <dbReference type="InParanoid" id="D5GI81"/>
    <dbReference type="OMA" id="TPWPVTI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008235">
        <property type="term" value="F:metalloexopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03875">
        <property type="entry name" value="M28_Fxna_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000057">
        <property type="entry name" value="Vacuolar membrane protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048024">
        <property type="entry name" value="Fxna-like_M28_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045175">
        <property type="entry name" value="M28_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053975">
        <property type="entry name" value="PFF1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053976">
        <property type="entry name" value="PFF1_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147">
        <property type="entry name" value="METALLOPEPTIDASE M28 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147:SF58">
        <property type="entry name" value="VACUOLAR MEMBRANE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22250">
        <property type="entry name" value="PFF1_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22251">
        <property type="entry name" value="PFF1_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0482">Metalloprotease</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0926">Vacuole</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000411746" description="Vacuolar membrane protease">
        <location>
            <begin position="1"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Name=1" evidence="3">
        <location>
            <begin position="13"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="topological domain" description="Vacuolar" evidence="1">
        <location>
            <begin position="34"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Name=2" evidence="3">
        <location>
            <begin position="382"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="1">
        <location>
            <begin position="403"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Name=3" evidence="3">
        <location>
            <begin position="439"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="topological domain" description="Vacuolar" evidence="1">
        <location>
            <begin position="460"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Name=4" evidence="3">
        <location>
            <begin position="470"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="1">
        <location>
            <begin position="491"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Name=5" evidence="3">
        <location>
            <begin position="506"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="topological domain" description="Vacuolar" evidence="1">
        <location>
            <begin position="527"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Name=6" evidence="3">
        <location>
            <begin position="532"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="1">
        <location>
            <begin position="553"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Name=7" evidence="3">
        <location>
            <begin position="663"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="topological domain" description="Vacuolar" evidence="1">
        <location>
            <begin position="684"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Name=8" evidence="3">
        <location>
            <begin position="699"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="1">
        <location>
            <begin position="720"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Name=9" evidence="3">
        <location>
            <begin position="728"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="topological domain" description="Vacuolar" evidence="1">
        <location>
            <begin position="749"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="571"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="581"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="216"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="315"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="2">
        <location>
            <position position="314"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="4">
        <location>
            <position position="125"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="4">
        <location>
            <position position="355"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="4">
        <location>
            <position position="840"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P38244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P80561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3"/>
    <evidence type="ECO:0000255" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="6"/>
    <sequence length="969" mass="106569" checksum="F4A545AF55AA9078" modified="2010-06-15" version="1">MTRVNSIIGFRPIPVTLLTVITYVSLFSALLFIDRQPPAVAKKGELDYWGVDIEEAWSDLEVLTRDFHPYNSRPNDDVRAYLLGRVQEILSRNGVSGFGNELQSSGYSGTVDLFDDGIPGKPGSNVTFVGAGSEDLTVYFEGTNIIVYIHGERPADELSPVLVSAHYDSVSTGYGATDDGAAVVSILQIIKSFTRPESQGGKRPKRGLVALLNNGEEDFLNGARAFAMHPVAKLPHSFLNLEGAGAGGRATLFRSTDAEVTKYYKRAKRPFGTIVSGDGFKAGLIRSGTDYSVFVENLGMRGLDVAFYQPRSRYHTTEDDARHSSKRSLWHMLGGSLATLRGMTDDTSKVFDSPNGSAGKGHNAVWFDLFGRAFSVLHLHTIFAFTITLIVVPFVVVLVAMWALGHFDKLYFFSNTAYIPPPPEHSIASRTTQGWRGVLRFPVAFVAASAGVVGMAFLINKINPMVVYASQYTVWTCFLSTWWIIAWVILRGADAVRPTALARGYGFLEQWLLWLVAMIGVAISIGKSHLGSGYWVLVFYSGFFTSAFISLLEMAALQKKSEVKIAVSGDDQAYPPEEHSQTGASGNISNRAANDDDDAGEHATEETPLFRGPNRPLSFAPHRNPRYDNGDHDSGDILVEEKDEAVYGGEQGWSKDLPSWTWILQFLATVPLQLVLAGSVALLLGNALAQTGADGSDMLTVLLGFGVFSIILLLPVAPFLHRITYHVTLFIFVIFVGTFIYNLAAPPFSPNARLKVYFQQTVDLQTGENNVHLVGHPDYIDRIVSKHIPSAKGKVDICQADKLKSGLRRCSWKGIAPRVVPQAPGGMPPEYGFSDWISYNVTKTGDGKATIKLRGRNTRACKLLFNKPLAKSPTLGNPIPGFPNTRELRLWSRDWERGWSVELTWEKEEDQEENKDSHSSAIVVCLWSDGNKVSDEIPALHEIRRYLPVWAIASKLADGLVEGSVPFMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5GID2</accession>
    <name>D5GID2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00008433001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430326">
        <property type="protein sequence ID" value="CAZ84275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840084.1">
        <property type="nucleotide sequence ID" value="XM_002840038.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GID2"/>
    <dbReference type="STRING" id="656061.D5GID2"/>
    <dbReference type="GeneID" id="9184633"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008433001"/>
    <dbReference type="eggNOG" id="ENOG502QPI7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_342956_0_0_1"/>
    <dbReference type="InParanoid" id="D5GID2"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43991:SF12">
        <property type="entry name" value="WD REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_8G05640)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43991">
        <property type="entry name" value="WD REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_8G05640)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="733"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="779"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="468"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="51"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="76"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ84275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="826" mass="91845" checksum="BC7F05565E96070A" modified="2010-06-15" version="1">MESIEVTRSPLPLPPTLHVPNPIDEDEEDFSPSNLVTRPPSPVGGGAAFLDFFSSSPSSTDPSTHEGPSDNGSENANDDEAPDDDPVFLMQLDPPLEEFGSVQDHSSPPAAIYSDSYVHSGAFGVHGSMMDPLSEILVTRSFEGQQEGSTMEDEALESEASQTPGWNENIENYNYDFAKFCLHAYYRYRMNPARYQKLSVAAADVKKLPRPREITRRDVERDGCDIQAIPWQKLGITVDVARGVRRKEYINYRNEKDVEPEQPPSIPANRQFYKFRRMDMEQKPRLIHFQLRNLLGVVSRSDVFYAGNAKVLRTNPLTGSTVTTMDLSYPDSWSGNQIRISTLSACAGQSGVVIAGCFHGEYAMKPLFAQASAKPITGIVTKNESGITNHVQIVRNRHNNTPNAVFSSNDCHVRVLDCETLAFNTQHEYDWPVNCSVTSPDSRLRVVVGDHTDVLICDAERGSTQFVLPGHRDYGFAAAWSDDGHTIATGNQDETVRIWDSRKLNETLKVLPAKMAGVRALRFSPLGYGGKRVLAMAEPADIVQIVDATSWDSMQQLEFWGEVGGIEFAPTGEEFFIANADKSVGGLMEFERCKGRAYYQEYPEGNSGREVLCWEDAMDEEGDMNGPGRRGRGSNIISDGWKAEEEHSSCCFILSISLSDCLFVCLPSSSLPYFSKHIQPHSTHLAPFYTGAICNNHHLPLLPFLSLPFFFLPSPSPSPPSPSSINTRGLSDFIIITFVASHDCLFSSFITLFSWLLSSRLSCLLVHATLRYSSLRGKLAFCLSYFGFWIFFFGGSAYLLLGWVCRYYVRYGNEQDDEELPSGKGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIE5</accession>
    <name>D5GIE5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Carbon catabolite repression protein B</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Deubiquitinating enzyme creB</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Ubiquitin thioesterase creB</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Ubiquitin-hydrolyzing enzyme creB</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Ubiquitin-specific-processing protease creB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">GSTUM_00008451001</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Ubiquitin thioesterase component of the regulatory network controlling carbon source utilization through ubiquitination and deubiquitination involving creA, creB, creC, creD and acrB. Deubiquitinates the creA catabolic repressor and the quinate permease qutD. Also plays a role in response to carbon starvation and the control of extracellular proteases activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Interacts with creA, creC and qutD.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="FN430326">
        <property type="protein sequence ID" value="CAZ84288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840097.1">
        <property type="nucleotide sequence ID" value="XM_002840051.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIE5"/>
    <dbReference type="STRING" id="656061.D5GIE5"/>
    <dbReference type="MEROPS" id="C19.008"/>
    <dbReference type="GeneID" id="9184646"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008451001"/>
    <dbReference type="eggNOG" id="KOG1864">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_419317_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIE5"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02663">
        <property type="entry name" value="Peptidase_C19G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000075">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase creB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF733">
        <property type="entry name" value="RE52890P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="USP" evidence="15">
        <location>
            <begin position="20"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="419"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="419"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="462"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="485"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="502"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="520"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="545"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="588"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="616"/>
            <end position="630"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00042086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00042958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAZ84288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="654" mass="72853" checksum="C314E0397C804B6A" modified="2010-06-15" version="1">MENVVGVCPNFFIIVLCENHWLMLDSIFSYCNSILQCLYYSKPFRENVGNHASYGMEESLFSALKDIFESIVAHNSRTGVVSPNRFLDILRRENDMFSGQQHQDAHEFLNYVLNQVIENVEDYQKKHGYKNGNIVTNSTPAKSSGTLTPVNASSAASTASLTSTSPTGWIHELFEGLLTSETKCLTCENVSRRDEQFLDLSIDLEKNSSVTSCLRNFSASEMLCERNKFHCDSCGGLQEAEKRMKIKRLPKVLALHLKRFKFMENLGRFEKLHYRVLYPYHLRLFNTTDDAEDPDRLYELYAVVVHIGGGPYHGHYVAIVKTEDKGWLLFDDELVEPVDKAFVRNFFGERPGMACAYVLFYQETTFEAVQREMWVDEQAAGVATRAAAEAAKTESAKSKGYTNGVNGHSVRASSVAEHLPNLHSSSSSPLPKSETPINISDQHPAETAAKHPFPPVPSSVKSKKEKEKGDKKDKPEGYHMGGINRLRSTSRSLRLSQALRLGGKDKDRTSTHEENGAIPDLPPFPVTPGPGPGLPVGDLTPNGVNHEKSDDEKSGDDSPSGTEVPSAVESPKVTVPAVAPAKLGESPVEPKEEVVVPQETERGDKKENSKKKKGKKEKEKKEKKEKEKEKNKARHMSSFFGLRKKASVAFHSES</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-10-19" modified="2025-06-18" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIH1</accession>
    <name>GATA_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamyl-tRNA(Gln) amidotransferase subunit A, mitochondrial</fullName>
            <shortName evidence="1">Glu-AdT subunit A</shortName>
            <ecNumber evidence="1">6.3.5.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ORF">GSTUM_00008496001</name>
    </gene>
    <organism>
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutamyl-tRNA(Gln) + L-glutamine + ATP + H2O = L-glutaminyl-tRNA(Gln) + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Subunit of the heterotrimeric GatCAB amidotransferase (AdT) complex, composed of A, B and C subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the amidase family. GatA subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.7" evidence="1"/>
    <dbReference type="EMBL" id="FN430326">
        <property type="protein sequence ID" value="CAZ84314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840123.1">
        <property type="nucleotide sequence ID" value="XM_002840077.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIH1"/>
    <dbReference type="SMR" id="D5GIH1"/>
    <dbReference type="FunCoup" id="D5GIH1">
        <property type="interactions" value="318"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GIH1"/>
    <dbReference type="EnsemblFungi" id="CAZ84314">
        <property type="protein sequence ID" value="CAZ84314"/>
        <property type="gene ID" value="GSTUM_00008496001"/>
    </dbReference>
    <dbReference type="GeneID" id="9184672"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008496001"/>
    <dbReference type="eggNOG" id="KOG1211">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009600_7_6_1"/>
    <dbReference type="InParanoid" id="D5GIH1"/>
    <dbReference type="OMA" id="QPASYCG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030956">
        <property type="term" value="C:glutamyl-tRNA(Gln) amidotransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070681">
        <property type="term" value="P:glutaminyl-tRNAGln biosynthesis via transamidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00120">
        <property type="entry name" value="GatA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000120">
        <property type="entry name" value="Amidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004412">
        <property type="entry name" value="GatA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895:SF7">
        <property type="entry name" value="GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT A, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895">
        <property type="entry name" value="TRANSAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0496">Mitochondrion</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000413358" description="Glutamyl-tRNA(Gln) amidotransferase subunit A, mitochondrial">
        <location>
            <begin position="1"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="205"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="218"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="62"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="140"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="1">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="488" mass="52591" checksum="56D14BC0C58C687B" modified="2010-06-15" version="1">MALLEIAKKSLLAAEKYLALNSLISVQSQPELLSQVQASQERISADSGESKSSIDGQLIAVKDNICTTDFPTTCASKLLEQYVSPFDATVVRKAKEAGAVIMGKTNMDEFGMGSHSMYSHFGPVMQSAEDGRPARSAGGSSGGSAVAVATGMCYAALGTDTGGSVRLPAAYCGIVGFKPSYGMLSRWGVVAYANSLDTLYDAINGHDDNDPTSITPQTRERIQDRLSRSRRRGQRLAIGYPEEFNLVELDDPVRDAWENTLAHLNSQSHRIAPVSLPHTKHALSAYYVLAPAEASSNLAKYDGVRYGYRDPADRSQDGLLFSPTRANFGAEVRRRIIAGAYSLSSEAIDNYFLKAQSARRIIQRDFSNAFAQPNYLITDNLEEGRGKEGVDVLIAPCSLSKAPFLEDVKMQKSALDSYVNDVLTVPASLAGIPAICLPVMHGENDPVGIQVMAQYGDEETMWEVAKIIEEGLGSGVGSKTQPTNPTVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIP1</accession>
    <name>D5GIP1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00008592001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430328">
        <property type="protein sequence ID" value="CAZ84384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840193.1">
        <property type="nucleotide sequence ID" value="XM_002840147.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIP1"/>
    <dbReference type="GeneID" id="9183318"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008592001"/>
    <dbReference type="eggNOG" id="ENOG502S8TF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_785714_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIP1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052207">
        <property type="entry name" value="Max-like/E-box_TFs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15741">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX ZIP TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15741:SF27">
        <property type="entry name" value="TRANSCRIPTION FACTOR AP-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="7">
        <location>
            <begin position="279"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="193"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="236"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="270"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ84384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="353" mass="39204" checksum="627230E4EEDA63CB" modified="2010-06-15" version="1">MASSRSTFDFYGFEARFNASSPPAPVGPPLLDTHEQKGLENWFESIGSGSVASSNLFDTELPDDSPYWSSDMPSSYQNTIPEMPSRHHHHQYLSGGMDGYLTSSSVSAAPMAAPSQIDINAFNTSRGLLQRQQGQTSQQLNNHNLLNFGTDTNFAPNGYHPPTLPVPLEDKEVEVRSRVYAAFRNDSTVTTAVNSPTEIKYEQGGGDTEDDDSGTPVYDDNESTPSPSRNSAMLKRRMDGGDDFSKSARKPRSRKSEASMRMAKKKAGGQKRDNLTEAQKRENHIHSEQKRRNLIRQGFEDLCSLVPELSSGGYSKSAVLVHAANYLDDLKKGNVRLRLYLQQLQQLQMDRGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJH7</accession>
    <name>D5GJH7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00009022001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430331">
        <property type="protein sequence ID" value="CAZ84670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840479.1">
        <property type="nucleotide sequence ID" value="XM_002840433.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJH7"/>
    <dbReference type="GeneID" id="9182671"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009022001"/>
    <dbReference type="eggNOG" id="ENOG502S2VF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028200_2_1_1"/>
    <dbReference type="InParanoid" id="D5GJH7"/>
    <dbReference type="OMA" id="YRRITWA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF146">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="28"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="71"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="108"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="142"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="8">
        <location>
            <begin position="7"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="206"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ84670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="250" mass="27718" checksum="B99D05E27A42FD17" modified="2010-06-15" version="1">MLGVLGFKVSLCFAYLRLVEKRRVYRRITWAVLITCTLAHIGGTLVLMFQCNPVYKSWSPRTPGKCLRNDITFYTLAAITIFYDLCVIILPIPLFLRTLITNRKKAVLIILFALFGFTTVCSVMRMVQILTIAKNGNSTMLVLWGTIEMNVGITTTCIPTLSPLFKFFQERTSAKASSGYTSGASKASRKKNIELTFMGQLPRETSAYVTSSRRSNSEERILGAGEESGIGVKTDIQVEVSSAHSQGSQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJY2</accession>
    <name>D5GJY2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Mediator of RNA polymerase II transcription subunit 19</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Mediator complex subunit 19</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MED19</name>
        <name evidence="11" type="ORF">GSTUM_00009265001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Mediator complex subunit 19 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430334">
        <property type="protein sequence ID" value="CAZ84825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840634.1">
        <property type="nucleotide sequence ID" value="XM_002840588.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJY2"/>
    <dbReference type="STRING" id="656061.D5GJY2"/>
    <dbReference type="GeneID" id="9186861"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009265001"/>
    <dbReference type="eggNOG" id="ENOG502QXG3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036911_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJY2"/>
    <dbReference type="OMA" id="TSAFHLC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013942">
        <property type="entry name" value="Mediator_Med19_fun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28270">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28270:SF1">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08633">
        <property type="entry name" value="Rox3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0010">Activator</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="241"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="34"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="57"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="302"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ84825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="351" mass="36695" checksum="62A7929439C21BD4" modified="2010-06-15" version="1">MTSSFSGGSRSLHPTNTIPTPASSAANTVPATFEDNEVDVMMDEGSDTGSNKRRRTSSSSYDRASQTMPPALRHRGNLADDIPPPIPTEQELLSQPDVSPYYLVCSNPRQPSYPDLTDNLLALYGLRDIANSVARKNHITGAKNKIRKSYKGFTSDLPGRNVVATRASQTEVEPGGEAGGGGGDYFATLLQFPDEEWRNLQVIGKEISKGIDMGKLRKGLSMGRGDIPGFDSSILGLDEESRGRKTPHVGSTTPAVGAAVGTPGHANGSTPGGPSPAGDDGRPKRGKRRRNDGGDDDNGINGSIGGVAGGGGGGNHGQGPGVGPGAIVEGTAWDGEKRKKKRKKVNTLLNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJZ1</accession>
    <name>D5GJZ1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00009280001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430335">
        <property type="protein sequence ID" value="CAZ84834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840643.1">
        <property type="nucleotide sequence ID" value="XM_002840597.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJZ1"/>
    <dbReference type="STRING" id="656061.D5GJZ1"/>
    <dbReference type="GeneID" id="9186870"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009280001"/>
    <dbReference type="eggNOG" id="ENOG502QXAC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021696_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJZ1"/>
    <dbReference type="OMA" id="CERCVWE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050869">
        <property type="entry name" value="H3K4_H4K5_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12197">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SMYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12197:SF273">
        <property type="entry name" value="MYND-TYPE ZINC FINGER PROTEIN SAMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ84834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="502" mass="57100" checksum="0B64699B887ADD99" modified="2010-06-15" version="1">MHMRDSDAALAGAYPESRSSDSLLEQARSATLKGECVRARKLYTEVLMLEPHSILAYLERSLAHLRLAFPDLAAGDAYRALLLVDMARECDEEITPEIAEMEAKTFSCLSIALREIDCHEDSMRYEEIGTKRYPEFNFEVPGHTPRKEATLREGEWRREVYPWNNMEPDRFSDKTLEVLNAAMAKCSAKAELKVVDLPVLQRDLSFAGDGDRSSDKHLGVYAKEDIFLGETFFSEASPLTVVADPENSSLCEYCGGNLPDQYEVCDDCWEGDEDSGVMWCSEECRQKAFKSYHPALCGRDFTWLYRDVNATANVHPLALPDVKYLYGVSRPPLKHIPAIALPFNFTHQVLQPIQMLQEMDVDIFDPKYLDFFDLWVMQTVRAKFISVASARISSKTRRTELAAVHALYSMFNHSCEPTVTWECESEVKFTGFSRHKKPSESAAPIVAVKKGDECWTHYCDVRMEYKDRQRDAFGSLGGKCVCRRCVEEEAEDKRVNGVPDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKG7</accession>
    <name>D5GKG7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00009541001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430338">
        <property type="protein sequence ID" value="CAZ85010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840819.1">
        <property type="nucleotide sequence ID" value="XM_002840773.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKG7"/>
    <dbReference type="GeneID" id="9182030"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009541001"/>
    <dbReference type="HOGENOM" id="CLU_3070387_0_0_1"/>
    <dbReference type="InParanoid" id="D5GKG7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ85010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="53" mass="6256" checksum="7037F4C0D322814E" modified="2010-06-15" version="1">MSPHISLDYHIYTLVQTSFIKALTVRKPRSVPLTLPYYFRRDNTKTTKRNGDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKH0</accession>
    <name>D5GKH0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00009555001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430339">
        <property type="protein sequence ID" value="CAZ85013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840822.1">
        <property type="nucleotide sequence ID" value="XM_002840776.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKH0"/>
    <dbReference type="GeneID" id="9182032"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009555001"/>
    <dbReference type="HOGENOM" id="CLU_2887468_0_0_1"/>
    <dbReference type="InParanoid" id="D5GKH0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ85013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="63" mass="7392" checksum="3839144024F0AA4B" modified="2010-06-15" version="1">MGQVIQVRLWMELYDLCTKCRSRITAAACLAVVCSYCGYHMIHRRIVHVIVRMPLLSSFHRVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKL8</accession>
    <name>D5GKL8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA-directed RNA polymerase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00009634001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">DNA-dependent RNA polymerase which catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430340">
        <property type="protein sequence ID" value="CAZ85061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840870.1">
        <property type="nucleotide sequence ID" value="XM_002840824.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKL8"/>
    <dbReference type="FunCoup" id="D5GKL8">
        <property type="interactions" value="699"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GKL8"/>
    <dbReference type="GeneID" id="9184723"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009634001"/>
    <dbReference type="eggNOG" id="KOG3297">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073901_1_0_1"/>
    <dbReference type="InParanoid" id="D5GKL8"/>
    <dbReference type="OMA" id="LGPTLWW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006386">
        <property type="term" value="P:termination of RNA polymerase III transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04330">
        <property type="entry name" value="RNAP_III_Rpc25_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000221">
        <property type="entry name" value="DNA-directed RNA polymerase III subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.120">
        <property type="entry name" value="RNA polymerase Rpb7-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013238">
        <property type="entry name" value="RNA_pol_III_Rbc25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036898">
        <property type="entry name" value="RNA_pol_Rpb7-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004519">
        <property type="entry name" value="RNAP_E/RPC8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045113">
        <property type="entry name" value="Rpb7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005576">
        <property type="entry name" value="Rpb7-like_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00448">
        <property type="entry name" value="rpoE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II, III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709:SF1">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08292">
        <property type="entry name" value="RNA_pol_Rbc25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03876">
        <property type="entry name" value="SHS2_Rpb7-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88798">
        <property type="entry name" value="N-terminal, heterodimerisation domain of RBP7 (RpoE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="RNA polymerase Rpb7-like N-terminal" evidence="7">
        <location>
            <begin position="9"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase III subunit Rpc25" evidence="8">
        <location>
            <begin position="82"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU369086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ85061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="194" mass="21739" checksum="251E84654CF05985" modified="2010-06-15" version="1">MFILSKFTDLLHLPPPTFAHPTATALEDQINEKYANKVVHNIGLCISLYDLSSVSEGMIGQDGGAHVNVEFRMIVFRPFKGEVLTGRISSATAAGVKVRTDFFDEVFVPAAALFEGSRFDGKEQVWIWRNDDQDFYMDKNEVIRFRVEGEVFVDQLPVPPHLKGEESSLHNKPPYAITVGFSSALLMCSQLWAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKR1</accession>
    <name>D5GKR1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">S-formylglutathione hydrolase</fullName>
            <ecNumber evidence="2 7">3.1.2.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00009714001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Serine hydrolase involved in the detoxification of formaldehyde.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>S-formylglutathione + H2O = formate + glutathione + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14961"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:57688"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="EC" id="3.1.2.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the esterase D family.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.12" evidence="2 7"/>
    <dbReference type="EMBL" id="FN430340">
        <property type="protein sequence ID" value="CAZ85104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840913.1">
        <property type="nucleotide sequence ID" value="XM_002840867.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKR1"/>
    <dbReference type="FunCoup" id="D5GKR1">
        <property type="interactions" value="643"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GKR1"/>
    <dbReference type="GeneID" id="9183340"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009714001"/>
    <dbReference type="eggNOG" id="KOG3101">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056472_0_1_1"/>
    <dbReference type="InParanoid" id="D5GKR1"/>
    <dbReference type="OMA" id="PSDCPWG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018738">
        <property type="term" value="F:S-formylglutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046294">
        <property type="term" value="P:formaldehyde catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000002">
        <property type="entry name" value="S-formylglutathione hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000801">
        <property type="entry name" value="Esterase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014186">
        <property type="entry name" value="S-formylglutathione_hydrol"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02821">
        <property type="entry name" value="fghA_ester_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10061">
        <property type="entry name" value="S-FORMYLGLUTATHIONE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10061:SF0">
        <property type="entry name" value="S-FORMYLGLUTATHIONE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00756">
        <property type="entry name" value="Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0719">Serine esterase</keyword>
    <feature type="active site" description="Charge relay system" evidence="6">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6">
        <location>
            <position position="232"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6">
        <location>
            <position position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR614186-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ85104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="286" mass="31706" checksum="1187585A95D408DF" modified="2010-06-15" version="1">MAFEIKAKIANFGGQLLKLEHQSTSTKTKMALNLFLPPTPISHPSDKIPVLIFLSGLTCTGDNCPEKGFLQPHAVKHGIAVVYPDTSPRGAKVEGEDDNWDFGSGAGFYVDATTERWRNNYNMYTYINEELPLELFKQFEVLDSGKVGITGHSMGGHGALTIFLKNPGKYLSVSAFAPISNPINCPWGQKAFSGYFGEENKEKWAEHDATELVKSWEENETLEALIDVGTADNFYKQGQLLPENFKKAAGGKGVQVRYQEGYDHSYFFISSFAGDHVGFHAKYLCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKX9</accession>
    <name>D5GKX9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00009823001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430341">
        <property type="protein sequence ID" value="CAZ85172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840981.1">
        <property type="nucleotide sequence ID" value="XM_002840935.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKX9"/>
    <dbReference type="STRING" id="656061.D5GKX9"/>
    <dbReference type="GeneID" id="9184766"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009823001"/>
    <dbReference type="eggNOG" id="ENOG502SF1H">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_271172_0_0_1"/>
    <dbReference type="InParanoid" id="D5GKX9"/>
    <dbReference type="OMA" id="HLIIQRH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055781">
        <property type="entry name" value="DUF7357"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24054">
        <property type="entry name" value="DUF7357"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7357" evidence="2">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="261"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="333"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="505"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="631"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="737"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="938"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1016"/>
            <end position="1197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="357"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="402"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="417"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="465"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="505"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="532"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="634"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="748"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="944"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1059"/>
            <end position="1079"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1085"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1132"/>
            <end position="1143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1147"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1171"/>
            <end position="1189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ85172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1197" mass="131084" checksum="A97B930110B3F024" modified="2010-06-15" version="1">MRLRLFIQRNRLPSTKIWWDTSSFEPGDDHNVSTLLSRVTQIVPMESGGWGLEDYAVEVDGFEVLHFQEVARVIREGDQVVIRPLTTTEIRQRSASGRRQISTSGRKLLDGTPFGRPMVTTRPAPDRPRLSIPPMKRAKTGCDVDYSTLVQVGSDHEDESYEECDEEQDVSEIGDGDDGEAVGANMVAEVEACAELDEAEESGEEGEDDIDGADFEELSRDYENPLMDYFDCDEVASSQVTFFEHPSQLKAICGARDTQSLQGQNGSLGEDKSDDGDSYTDSDSSQYRDSDLSDVDDSSDKFVCGDESMEVGIVGRTGNLFLPDYFHSAASAAGGAVDSPTLPQLYQSEDTSEEAGGDERERELEHPNLSKPSVPAGPELDEDSRSSSEDSYSSSDSEESGESEKGAVPKDKRCVKSASQSKAPSTSQVPLPSRVTSFPIEQSKKLAVAPGTLPHQGNPGTKARNERKRQRRKNRRLLAELVNTGLLPKESTIDDLKTHRDKHLKNADEQLRHNNEHPLEGSGEKQTIFDLSEERVAEEPTRDLAGHALQKARQAQQSDDLLCKGARGKSWRRRGRRAFLKAKKRESIPADWDFSRWLTEREKKVGVEDQPNNIDVGEDADGDVSIVSIGKPATLSSKTPSKQIGISLSHDRPPPPPPHQDPLISAQESSRPQGPRKIIYDKDGAPNPVYAGFDEQRYGVEDPEAWRRKIVLSAVECEQEGVEIPAPGFPFKQPTYQPGFRWEASGNNKRKRSSSVKKGKRGQRGKENFSFDYGCRGYQDLGYGDWDGQDYYDDRTVAPTPAEGKATDGAEVDLVDDLPPLPENVASLYPLTKSVLPGTIVAFKQLMLTEDYTPIIADYRTAIVQAVHVQEKDGPALELRLAVRDRPKRRIDPETGERILRKFEMLGDENEEEGFLSLMFGQLLEAKVVKLPVGAGPGQSVDGDAGGGIGEGGGTQEGSPGDQRNDGNVNHDMSEATDSYPHNIQVNATLSDGLAPPPEEGSQDQEQDLELELGPEMQAAQPQESLKSPSPVDGILGTGVPLSSAPTSQAEASVPPAVDNEDSDPSYEPDSVSDSDGLETLESMFASSQRIKPEPSSQRSPVLPPLPVFSPSGTGVEEDDEQSQRQIQEEATNEKRTALEATRRTKASNSAKITNSGVQIIDLTETSDPIVMHDDPRTDEGGRSSEVRGKQWRAVKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKY6</accession>
    <name>D5GKY6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00009837001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430342">
        <property type="protein sequence ID" value="CAZ85179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840988.1">
        <property type="nucleotide sequence ID" value="XM_002840942.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKY6"/>
    <dbReference type="STRING" id="656061.D5GKY6"/>
    <dbReference type="GeneID" id="9185773"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009837001"/>
    <dbReference type="eggNOG" id="ENOG502S696">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048446_0_0_1"/>
    <dbReference type="InParanoid" id="D5GKY6"/>
    <dbReference type="OMA" id="MKVIIVR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ85179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="416" mass="44913" checksum="6D545EA78D3D9C8E" modified="2010-06-15" version="1">MPALDSSALLVARFLQSNGYEKSLKVFLEESNLDENSLDPGPAGGLTIEKVLDEKRQFDLSLSLENVVIADNDEPEFSIPYPSIPHVVSDPGILASNVLFIVVVDIRALGGPTLIVTTADRALRIYSATVPHCLLRSYTHLHSSAILCCTAIHSKWLISTSMSGHVVISDAASGDVISSDKPHAKYAVKILVHEKLPYAATSGYDNTIVLHSLTCGDDGTPKLLELHRLRFAAHPEGISFVALPTGEEALVYSLRDSTFLIYHLLAPSLPVHSKRNLSPTTTSWVTFHAMFILPHPNDSSLLGIVTSSVPHMKFLLVRVDSEEVIMEVFTGAPQSVYSTAVFGWRAGGDGVWVNGDDGVVRGIERKTGKVMARLRASEGSEKVRSLWTGVVGGQEVLVTGGFDKGIKVWVPEGVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GL84</accession>
    <name>D5GL84_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Vacuolar membrane-associated protein IML1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Vacuolar membrane-associated protein iml1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00010056001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IML1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430346">
        <property type="protein sequence ID" value="CAZ85277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841086.1">
        <property type="nucleotide sequence ID" value="XM_002841040.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5GL84">
        <property type="interactions" value="610"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GL84"/>
    <dbReference type="GeneID" id="9182071"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010056001"/>
    <dbReference type="eggNOG" id="KOG3572">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000935_1_0_1"/>
    <dbReference type="InParanoid" id="D5GL84"/>
    <dbReference type="OMA" id="PRARCYE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990130">
        <property type="term" value="C:GATOR1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010508">
        <property type="term" value="P:positive regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04449">
        <property type="entry name" value="DEP_DEPDC5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000591">
        <property type="entry name" value="DEP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027244">
        <property type="entry name" value="IML1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048255">
        <property type="entry name" value="IML1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13179">
        <property type="entry name" value="DEP DOMAIN CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13179:SF8">
        <property type="entry name" value="GATOR COMPLEX PROTEIN DEPDC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00610">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12257">
        <property type="entry name" value="IML1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00049">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50186">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEP" evidence="6">
        <location>
            <begin position="903"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="216"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="327"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="421"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1019"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="327"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="351"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1019"/>
            <end position="1037"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ85277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1379" mass="153379" checksum="509FBE623C0E2EB1" modified="2010-06-15" version="1">MIITGRPSAAIHGNILEAINLATLQFSKDYVDRDLVRTGVSVVIITPGTGHFEVDYDMLKATTEGLIMTGMGVDLVCLSKVPLHTVPLFKYRSPIPISILAKQEQGSPNGFPRGLSPPKVTVTSSGATAPDRSQGEWVYAVPHWIDISFWSAASEKKINKRGRVEKKKERKKSTGGFRARAKMYELQMMGIMENEISEISIPFLHDNPFWKPLPTEEEQAQTDGSNAKASAKPGTATSTAAPAKFLKEAFKWMDEYDDAAFRPLPVLKARERAALEHRIDGEDAEKIEKTLEEVDPLVLGTSFHGEAPGRQVSLPGGAFFDRKMKERRPELESPIESREEIPNIPNTSVTSAPSAAPPSSGLLRPSRLGLVRNISFGFKSWGAAKATAKTGIVSTKATAIAAGSPGAGSSGVMVTRGFDTSEAATPRSPLSPRTTHLEESPTRTGSLRSPEKLGWGSRPISIKNNAITSLEAAGRDRDRRRSIVGSPLDRASMLRERERESVDILKAASLTRHGRPSIDLVANAEKTATIPPTVSPTSALSPWVQVLNPSHPKTNIPSAVNQFRRWHHVFPKPIKISTVKWKSLCSPAALPLTTEHFPSAEQLRDEYQENPYVISQNADYDEIEESSSSNREELVRAMISQRLAQGFQIVVGSSVSEATAGRGGDADIYSKTYMVKAGSSCFMSLGSQIHQLICDEEYNVEVKRYVRKPTTAIGLSKFTIADEYISYMKTVMSEEYIPVKTAFRFPIAEYNWNYVDQYIGGYEDTLTEQLRYWRARFVLIPNDPPESARRGGLLGHSNGGELNDEEIRLEGIRRLTQVFQRNRYIPPEERQYERYGRRRAKEKNPLQILYKTVDPSVVVAQELESLPLIEDPTNNMRRSQLLTTEMFEKKNLDLKAIAQELQGPKGVRLQDRRWHLRFHSNCFIGEDMVTWIVENFKDVETRQEAEEIGLKLFKAGLFQHVDKRHQFRDGNYFYRIAEAYANTPRPTSKGGWFGGFKSDKSVPSTPAFDIAPSPLRSRSSTIVTDECSETSSMSGYRTPTPTPPTKKKVEVELSKVMKYDIDPSRKSYRKELINLHYDRLHNPDNCYHIRIDWMNTTAKLIEDQLASWARTVDRYGLKLVEAPMDEVSTIPRTSPFRSPYIFKFCLPPPPPPMLSTISPTTESMDLSTILCPTQPVPPPHHTHNQNDHHFYHRLALRKLGFVLDTEAAKNFPPTVSVKYSWGTPNYRHSQYIHRSGVMFCQITDDGSFLLMANRQYTIRATTIYPPPAVALGSGADVALETVKDEIESFCKDPEALRRFYDEANGRVGGGTAMGGSSMGTLGSPVIGPVGAGSPTMGTSVVHAHGVGRTTSMKSGRSSIVDPVPDFVLGRGGYEPGPGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLA1</accession>
    <name>D5GLA1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00010090001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430347">
        <property type="protein sequence ID" value="CAZ85294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841103.1">
        <property type="nucleotide sequence ID" value="XM_002841057.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLA1"/>
    <dbReference type="GeneID" id="9182088"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010090001"/>
    <dbReference type="HOGENOM" id="CLU_1846568_0_0_1"/>
    <dbReference type="InParanoid" id="D5GLA1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="114"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ85294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="139" mass="15469" checksum="5A84CD6925472B10" modified="2010-06-15" version="1">MLRLIINKQTFKAISIHPYRPHRGFSTVPGRNTDSDSHHDNDYHHDSSGGCAGSGSPPGVTSKDFANLDKDVSNIKLKLDYIQSGQSEMRADIKDLGNKTDKKFDKTDSKIDRIFYGVITTLVGFVLKGGFDYYQAAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLJ9</accession>
    <name>D5GLJ9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00010242001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430349">
        <property type="protein sequence ID" value="CAZ85392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841201.1">
        <property type="nucleotide sequence ID" value="XM_002841155.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLJ9"/>
    <dbReference type="GeneID" id="9186136"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010242001"/>
    <dbReference type="eggNOG" id="KOG0475">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003181_2_0_1"/>
    <dbReference type="InParanoid" id="D5GLJ9"/>
    <dbReference type="OMA" id="TDWVHDT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005247">
        <property type="term" value="F:voltage-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03684">
        <property type="entry name" value="ClC_3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3080.10:FF:000013">
        <property type="entry name" value="Voltage-gated chloride channel (ClcA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3080.10">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014743">
        <property type="entry name" value="Cl-channel_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001807">
        <property type="entry name" value="ClC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45711">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45711:SF3">
        <property type="entry name" value="CLC CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00654">
        <property type="entry name" value="Voltage_CLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00762">
        <property type="entry name" value="CLCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81340">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0868">Chloride</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="149"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="234"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="374"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="448"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="489"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="546"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="572"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="65"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ85392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="864" mass="95147" checksum="5ADFEF5F78EF8569" modified="2010-06-15" version="1">MADDIGSMPGQRRVPERDLKAGRRSRRRLSASSRDKDTEQDEQSGLLGGSCSGDQRDYQTVPGTPRHRLSRHHSSHSPMASRPSRVPSFSQCLSRALSNYDLRDRNNESLLEDRVWYDQFTSTDWINDSIKDAHRVRALRARKDFRGRVYAWFDGAQGWVLVAIIGFVTASIAFFIDVTENVLFDYKEGYCTNGWYFSKKKCCLSDTTNEFCPMWRSWSQAITKSEVSTERIDYIAYVTLTVILATASSLLTMTTRTVYPGTLRIQTVDEDLSQILGGSSGAEAKAAVASPTVYYSAAGSGVAEVKVILSGFVLHGYLGLKTLVVKSFALILSVASGLSLGKEGPYVHIAACVGNIACRLFSKYNRNDAKRREVLSASAASGVAVAFGAPIGGVLFSLEEVSYFFPSKTLFRTFFCCIVAALSLKFLNPYGTNKIVLFEVRYTSDWHIFELFVFVFLGMCGGVYGAFFIKASKFWATTFRRNGFIKTHPVLELSLVALATGLVSYWNRFVKLAVSELLFELASPCSGNPSDHSGLCPKPDEIPSTILYLLVALAIKSVLTVVTFGTKVPAGVYVPTMVIGGIMGHVVGLAVEYAVYKFPHSPMFASCPVGGASSACVTPGVYALVCAGAVMCGVTRLSVTLAIILFEMTGSLDHVLPFSLAVLVSKWTADAIEPLSIYDLLTALNSYPYLDTKMRPVFSSQLSDITPRPRPERVIDITDSVYVPADELREKLQMLQAVGELDGGLPIVRNDILVGLIPAPDLEFALDNLQSSEQDELCCMEPKGEWWDGGSDDDSMRDPTYFSQYIDPAPVALERHSPMELVYECFAKLGLRYMCVLRDGQFAGVVHKKTFVKYLMEVTEGKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLM1</accession>
    <name>D5GLM1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00010268001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430349">
        <property type="protein sequence ID" value="CAZ85414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841223.1">
        <property type="nucleotide sequence ID" value="XM_002841177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLM1"/>
    <dbReference type="STRING" id="656061.D5GLM1"/>
    <dbReference type="GeneID" id="9182717"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010268001"/>
    <dbReference type="eggNOG" id="ENOG502RYR6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022491_3_3_1"/>
    <dbReference type="InParanoid" id="D5GLM1"/>
    <dbReference type="OMA" id="PTEMRYP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030435">
        <property type="term" value="P:sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3960">
        <property type="entry name" value="Velvet domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021740">
        <property type="entry name" value="Velvet"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037525">
        <property type="entry name" value="Velvet_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038491">
        <property type="entry name" value="Velvet_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33572:SF17">
        <property type="entry name" value="SEXUAL DEVELOPMENT REGULATOR VELC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33572">
        <property type="entry name" value="SPORE DEVELOPMENT REGULATOR VOSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11754">
        <property type="entry name" value="Velvet"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51821">
        <property type="entry name" value="VELVET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0749">Sporulation</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Velvet" evidence="7">
        <location>
            <begin position="227"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="65"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="402"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="94"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="110"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="138"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="186"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ85414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="439" mass="47468" checksum="C07EA79654B27F3E" modified="2010-06-15" version="1">MMSPGPPPQPIYPHQARNAIPSSSPMAQSPRPMPSAVFQPHNHPQLPPPIFGGSQVAAVHFSGPVLAPLANPCSGASAGGPVLPPPVQSQQPSTHIPPQNTLPPQHVPSPLQTRGKNEGNQVSNRFARLMPSQPFPSPASSGYPSPSPFSVDQSYGSRRFSGDSGPERRRSSISAMIPSPTYTAKRSIDEGFSDEHQQQQQPSGYGPRFTPASVEDVATTRREAPVQRSPGRYQIQVRQQPVAARACGFGERDRRVIDPPPIVQLIINDSAGNSDPTEMRYPFNVVHCTLWSTDGQSDETALIGMDKRQTRRLMGTLVSSPFGCFFCFPDLSCRTHGKYRLRFVLMRLEPSDLQPEGYTPIITETLSDIFTVYTAKEFPGMRPSTSLTKALKRQGCAISVKKGNEKALGGAPGGSGKDEGSGDDTPSTAAGKPKRRRKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLM2</accession>
    <name>D5GLM2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RNA methyltransferase</fullName>
            <ecNumber evidence="6">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00010269001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="6"/>
    <dbReference type="EMBL" id="FN430349">
        <property type="protein sequence ID" value="CAZ85415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLM2"/>
    <dbReference type="STRING" id="656061.D5GLM2"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010269001"/>
    <dbReference type="eggNOG" id="KOG2899">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004729_2_0_1"/>
    <dbReference type="InParanoid" id="D5GLM2"/>
    <dbReference type="OMA" id="KWIHLFH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017069">
        <property type="term" value="F:snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040031">
        <property type="term" value="P:snRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039772">
        <property type="entry name" value="Bin3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010675">
        <property type="entry name" value="Bin3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024160">
        <property type="entry name" value="BIN3_SAM-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12315:SF0">
        <property type="entry name" value="7SK SNRNA METHYLPHOSPHATE CAPPING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12315">
        <property type="entry name" value="BICOID-INTERACTING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06859">
        <property type="entry name" value="Bin3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51515">
        <property type="entry name" value="BIN3_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Bin3-type SAM" evidence="8">
        <location>
            <begin position="28"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ85415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="257" mass="27888" checksum="58CEED70A6445ABE" modified="2010-06-15" version="1">MSTKTGSYKTYQSHARHTGSNPAAHIPDPRLNLLPQEILKDATVLDIGCNNGAVAGHLLLRHAVKSVVGVDVDEELISKSRSHVAFALSRTRPDGGERAAADWYPVSSVRRHGCRIPPAEVRERARFWCGDYVTASGGAVASGYDVVLALSVVKWVHLAHGDPGLRRFFERVAGDVKPGGYFVLEPQAWESYARAVGKNPDLRAVFEGIRVQPGVAMEGLIAECGFRIVARWGIGEGEVGLRREVLLFRRLGGGSDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLW7</accession>
    <name>D5GLW7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00010448001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="FN430351">
        <property type="protein sequence ID" value="CAZ85534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841343.1">
        <property type="nucleotide sequence ID" value="XM_002841297.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLW7"/>
    <dbReference type="STRING" id="656061.D5GLW7"/>
    <dbReference type="GeneID" id="9187314"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010448001"/>
    <dbReference type="eggNOG" id="ENOG502S09W">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1377890_0_0_1"/>
    <dbReference type="InParanoid" id="D5GLW7"/>
    <dbReference type="OMA" id="CGAANWC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.10">
        <property type="entry name" value="Endochitinase-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001002">
        <property type="entry name" value="Chitin-bd_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036861">
        <property type="entry name" value="Endochitinase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47849">
        <property type="entry name" value="CHITIN-BINDING LECTIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47849:SF8">
        <property type="entry name" value="LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00187">
        <property type="entry name" value="Chitin_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00270">
        <property type="entry name" value="ChtBD1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57016">
        <property type="entry name" value="Plant lectins/antimicrobial peptides"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50941">
        <property type="entry name" value="CHIT_BIND_I_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="2 3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072830" evidence="4">
        <location>
            <begin position="19"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-1" evidence="5">
        <location>
            <begin position="18"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-1" evidence="5">
        <location>
            <begin position="161"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="21"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="30"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="35"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="172"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="177"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ85534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="206" mass="20423" checksum="E3AA26C88097543C" modified="2010-06-15" version="1">MTRLSLFVVLGLVTSVFAQNCGPSYGNQICAAGTCCSQYGWCDTSAAHCDPATCLKAYSGAGSSCAGSTGGGTPTGGGSFPSSVPSIDICGADSGGISCPGAGPGGFYYRCCSVNGHCGPKNGEQAQSDYCGAGCQPGFGVCDQRPVPPPPPPPQGTAGAEQNCGPIVNKRCGGGLCCSGSNFCGSGPDFCGAANWCQVDWGHCNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5GM24</accession>
    <name>D5GM24_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00010493001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL8 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430351">
        <property type="protein sequence ID" value="CAZ85567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841376.1">
        <property type="nucleotide sequence ID" value="XM_002841330.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GM24"/>
    <dbReference type="FunCoup" id="D5GM24">
        <property type="interactions" value="1064"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GM24"/>
    <dbReference type="GeneID" id="9187347"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010493001"/>
    <dbReference type="eggNOG" id="KOG3166">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055193_0_0_1"/>
    <dbReference type="InParanoid" id="D5GM24"/>
    <dbReference type="OMA" id="RMVKWPA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030684">
        <property type="term" value="C:preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000003">
        <property type="entry name" value="60S ribosomal protein L7a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004037">
        <property type="entry name" value="Ribosomal_eL8-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018492">
        <property type="entry name" value="Ribosomal_eL8/Nhp2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001921">
        <property type="entry name" value="Ribosomal_eL8_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00881">
        <property type="entry name" value="L7ARS6FAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00882">
        <property type="entry name" value="RIBOSOMALL7A"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01082">
        <property type="entry name" value="RIBOSOMAL_L7AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="6">
        <location>
            <begin position="183"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="75"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ85567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="323" mass="36789" checksum="440313EF68DD5A9B" modified="2010-06-15" version="1">MPACVIVYRNPVQNGIILYVYSITQRHTETRSRFPQYKKKIPPQPHLQTHGYHDVCVLTTIPNFNRNLIHYAPESVKRKKSHGRPTKNPLIEKRPRNYGIGQDIQPRRNLSRMVKWPEYVRLQRQKKVLNLRLKVPPAIAQFQNVLDKNLATQTFRLLNKYRPETKIEKKERLTKEAAAVADGKKKEDVSKKPYVVKYGLNHVVGLIENKKASLVLIPNDVDPIELVIFLPALCRKMGVPYAIIKGKARLGTVVHKKTAAVLAITEVRSEDKSELSKLITAVKEGYSDKYEESKRHWGGGIMGAKANAKVAKKQKALDAAIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="91" xmlns="https://uniprot.org/uniprot">
    <accession>D5GM33</accession>
    <name>D5GM33_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Alpha-aminoadipate reductase</fullName>
            <ecNumber evidence="6">1.2.1.31</ecNumber>
            <ecNumber evidence="5">1.2.1.95</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">L-aminoadipate-semialdehyde dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">GSTUM_00010502001</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the activation of alpha-aminoadipate by ATP-dependent adenylation and the reduction of activated alpha-aminoadipate by NADPH. The activated alpha-aminoadipate is bound to the phosphopantheinyl group of the enzyme itself before it is reduced to (S)-2-amino-6-oxohexanoate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>(S)-2-amino-6-oxohexanoate + AMP + diphosphate + NADP(+) = L-2-aminoadipate + ATP + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46936"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58321"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58672"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="1.2.1.95"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(S)-2-amino-6-oxohexanoate + NAD(+) + H2O = L-2-aminoadipate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:12308"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58321"/>
            <dbReference type="ChEBI" id="CHEBI:58672"/>
            <dbReference type="EC" id="1.2.1.31"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(S)-2-amino-6-oxohexanoate + NADP(+) + H2O = L-2-aminoadipate + NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:12304"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58321"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58672"/>
            <dbReference type="EC" id="1.2.1.31"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pantetheine 4'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:47942"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (fungal route): step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.31" evidence="6"/>
    <dbReference type="EC" id="1.2.1.95" evidence="5"/>
    <dbReference type="EMBL" id="FN430351">
        <property type="protein sequence ID" value="CAZ85576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841385.1">
        <property type="nucleotide sequence ID" value="XM_002841339.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5GM33">
        <property type="interactions" value="672"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GM33"/>
    <dbReference type="GeneID" id="9187360"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010502001"/>
    <dbReference type="eggNOG" id="KOG1178">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_19_1_1"/>
    <dbReference type="InParanoid" id="D5GM33"/>
    <dbReference type="OMA" id="ENDKFTM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004043">
        <property type="term" value="F:L-aminoadipate-semialdehyde dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031177">
        <property type="term" value="F:phosphopantetheine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05235">
        <property type="entry name" value="SDR_e1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000787">
        <property type="entry name" value="L-2-aminoadipate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.30">
        <property type="entry name" value="Nonribosomal peptide synthetase, condensation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013120">
        <property type="entry name" value="FAR_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020806">
        <property type="entry name" value="PKS_PP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006162">
        <property type="entry name" value="Ppantetheine_attach_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010080">
        <property type="entry name" value="Thioester_reductase-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01746">
        <property type="entry name" value="Thioester-redct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44845">
        <property type="entry name" value="CARRIER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44845:SF1">
        <property type="entry name" value="L-2-AMINOADIPATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07993">
        <property type="entry name" value="NAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001617">
        <property type="entry name" value="Alpha-AR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00823">
        <property type="entry name" value="PKS_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00012">
        <property type="entry name" value="PHOSPHOPANTETHEINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="10" id="KW-0521">NADP</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carrier" evidence="18">
        <location>
            <begin position="746"/>
            <end position="823"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAZ85576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1290" mass="141344" checksum="2136489ABFDF8A69" modified="2010-06-15" version="1">MRDSIDARLARWEERLRSLTTQSLTTDYVRPDPPRLVEAVHTVAVPEPAKLALLQLGILDGSNPVPPFTVLLTAFSVLAFRLTGDEDISIGTTAENKEPFILRTPLTGQTTFIDLLKQVKKLEIEGAADSVPFSQLVTHIQYKSKAQAPPCLYHLSLYHAPDAPSKQFLSSYELTTDLSIYVELNAPATSQSLRYVPQMPNITLNAHYNQLIFSRKRISFMVDQLLQIVQFGAKNPEISVGSIPLQTPEQVVILPDPRKDLNWGGFRGAIHDIFSANAKKHPDRECVVETPAFYEEDSKSRIFTYMQIDEASNILAHHFIAKGVKRGDVVMVYAYRGVDLVVAVMGVLKAGATFSVIDPAYPPARQNIYLQVAQPRALVPGLAIQNDGTLVGVVIGPDSTPTLSFTSGSEGIPKGVRGRHFSLTYYFPWMAEAFGLSENDRFTMLSGIAHDPIQRDIFTPLFLGAKLLVPTADDIGTPGRLAEWMDENCATVTHLTPAMGQLLSAQATRPIPSLHHAFFVGDILTKRDCLRLQSLARNVNVVNMYGTTETQRSVSYYEVPSLNQNATFSQTQKDVIAAGKGMLDVQLLVVNRHNREQICGSWRVSRRLSTYSRDDRHEIYQELRILYGSSRSLIQNRRPGGRADDQVKIRGFRIELGEIDTSLSQHPFVRENVTLTTDEGTYLKARLPSYAVPAVVIPLTRLPLNPNGKIDKPALPFPDTAQLAAAFRRTRRGTNAAADLRELEDQSLTPVQKDLRDIWQGLLTHSTTPIGLDDNFFDIGGHSILATRMIFEVRKKFVTDVPLGSIFKHPTLGALASEIERVKGSSELNIQGIGETSDTDPGAGKITDYSADAREIAKTLPGSFLAVKTPVLGGNVFLTGATGFLGVYLLRELLSRSNPAIGKVFVHVRAKNADAAMERVVGSCEAYGVWDPLWGNRIVAVVGELGSERLGLSEAEWGTLTEEVDVIIHNGAQVHWVYPYTKLKPANVTGTVDALQLCATGKPKSFAFISSTSVLDSDHYVRLSDSILETGGVGVPESDDLGGSAQGLRTGYGQSKWVGEYIVREAGRRGLDGCVIRPGYIVGDSKSGVTNTDDFLIRMIKGCAQLGQMPDIYNTVNMVPVDHVARVVVACAFNPPASPLPVAHVTSHPRLRFNEFLCTLATYGYNVTKVDYIPWRIALETHVVEKCEDNALYPLLHFVLDNLPSSTKAPELDDSNAVTALLSDQEWTGQDGSRGAGVCVEQMGIYLAYLIGIGFLPLPSGKGELELPVVELSDRQRGALESVGGRAGLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GM59</accession>
    <name>D5GM59_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">AMP deaminase</fullName>
            <ecNumber evidence="4">3.5.4.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Myoadenylate deaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00010538001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via salvage pathway; IMP from AMP: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the metallo-dependent hydrolases superfamily. Adenosine and AMP deaminases family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.6" evidence="4"/>
    <dbReference type="EMBL" id="FN430352">
        <property type="protein sequence ID" value="CAZ85602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841411.1">
        <property type="nucleotide sequence ID" value="XM_002841365.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GM59"/>
    <dbReference type="FunCoup" id="D5GM59">
        <property type="interactions" value="531"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GM59"/>
    <dbReference type="GeneID" id="9187386"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010538001"/>
    <dbReference type="eggNOG" id="KOG1096">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003782_0_0_1"/>
    <dbReference type="InParanoid" id="D5GM59"/>
    <dbReference type="OMA" id="GEPRVWP"/>
    <dbReference type="UniPathway" id="UPA00591">
        <property type="reaction ID" value="UER00663"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003876">
        <property type="term" value="F:AMP deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046033">
        <property type="term" value="P:AMP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032264">
        <property type="term" value="P:IMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01319">
        <property type="entry name" value="AMPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.800.20:FF:000001">
        <property type="entry name" value="AMP deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000214">
        <property type="entry name" value="AMP deaminase Amd1, putative (AFU_orthologue AFUA_8G02860)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000035">
        <property type="entry name" value="Probable amp deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.800.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006650">
        <property type="entry name" value="A/AMP_deam_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006329">
        <property type="entry name" value="AMPD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01429">
        <property type="entry name" value="AMP_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11359">
        <property type="entry name" value="AMP DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11359:SF0">
        <property type="entry name" value="AMP DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19326">
        <property type="entry name" value="AMP_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00485">
        <property type="entry name" value="A_DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="26"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="868"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="975"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="883"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="896"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="979"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1013"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1027"/>
            <end position="1037"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00072037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00078830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ85602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1037" mass="115139" checksum="51E5BE2F42FB8798" modified="2010-06-15" version="1">MPTPENAPPRDPSSEGSDQCRLNLRVTGASGVGWDDSDRLLDSPGDLRPPSPEDDHADQDSLGGSDEERKSAFYDYKQEKALKQTDAKMFYQQQQQANSGWNSPIMRASTGNWAGPGGSGGIGNPSRAGSVRSFTSSHQLPLHHHPHHQYLGSAAGGSTLGIAGSRSLANATPAPLGMVSLEQPKEHEGIQAPDMARFDPHGVVASAARQSGDSISPHGEILPQSQGGYIDRDPALSAELSTIYTNVQKILDIRHKYLRVSLQGHADNPKDDPSWKIYPPPPPPVWKEEANATAQKEARKPGQNIGGDFIFEECEIPGEDEMEFRKDGHGVFQVYENKKALDAGMPVVAIPTLREYYIDLDKVLDISSDGPSKSFAFRRLQYLEGKWNLYTLLNEHQEMADSKRVPHRDFYNVRKVDTHVHHSACMNQKHLLRFIKHKIRRCPDEKVLFRDGKHLTLTEVFASINLTAYDLSIDTLDMHAHTDSFHRFDKFNLKYNPVGESRLRTIFLKTDNDIKGRYLAEITKEVFQDLEASKYQMAEYRISIYGRAEDEWDKLAAWVVDNKLFSHNARWLIQVPRLYNVYKSTGLINSFDEVVRNIFKPLFEVTKDPSSHPKLHVFLQRVVGFDSVDDESKAERRTFRKFPSPPEWTVKSNPPYSYWIYYLFANTASLNVWRKQRGFNTFVVRPHCGEAGDTDHLAAAVLCCHSISHGILLRKVPLLQYIFYLDQIGIAMSPLSNNALFLAYDKNPFLSYFKRGLNVSLSTDDPLQFAFTKEPLIEEYSVAAQIYKLSAVDMCELAKNSVLQSGFEGAIKARWLGNDYHVPGPEGNNMDKSNVPNIRMAFRHQTLHEELRMLGRYTSAQAMANAAGPQGAGLAGRLTLPGSPFPGFRPPSPTTSMQTNPTSVQEPPSPQGDLPLPIQFPTSPGSSKLLASVHIHDLQGQTASPNPPGTESRDGYPSFPVPARANTTGTAALLRHHTSGSSGSPSGYGDYHISGSEPRVWPGVISRRQASISEKDCTNKGVENEAKGSNNGSVAGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>D5GM77</accession>
    <name>D5GM77_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00010558001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430352">
        <property type="protein sequence ID" value="CAZ85620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841429.1">
        <property type="nucleotide sequence ID" value="XM_002841383.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GM77"/>
    <dbReference type="GeneID" id="9187404"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010558001"/>
    <dbReference type="HOGENOM" id="CLU_432231_0_0_1"/>
    <dbReference type="InParanoid" id="D5GM77"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="316"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="325"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="350"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="365"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="390"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="419"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="633" mass="70978" checksum="4A3F2E6BB11CB71A" modified="2010-06-15" version="1">MTLRPTRPSWLFEEHELEELIEQSNPTISLSALRASRNECKLVDSVLKQILSVQNIQTTPGEFELYWAKTHLAMDIQSILKRWCSVGWDKTWEGSALRDLCLEAPKRHGDRDMDDARTTTIVGAPTDTTAEQKVGLLKSEPTRPRFPETPAARRQDSCSGWTPCNGPVVESVGAEVTAAAARRVYPIVSKRSLEDPDDPQARRVSRSKSVNFGDRTQFTCPYYARNFDEHPECANKSFPNPRKLKEHVWRWLKPFKCPTCGEGFGREKTRAIHCDQRKTKCKWMPSTYEGSAEQRRDLKIESAKSTKEMIEIFEEYEQEKHNPRDSNSPPSSTNSHNQDKNSNNDDDSTSDERHQVGEYRDNQDGRSSSPESQNASNASVSDGDSDHGDNYTTTKSNHTHTQPQQTSSARMGEGVGTDIDARRDSINNDEREEEEAVGLGLNPRHGFDHLNSNNARHSLSQDSFQYPLRQLSRDEDESRTLYLYPHQHQISPILRSSSDIDYSASNTPNTTTVLHPGAISTNILSSVPEIVVTHAEPRIKSPTITRSSDTNPYAQQTVYYPNPTACSSSNGLYNHHHHHGNFKGGEEDESSPCAENVPYGLNSRGLGQGYVAAPFITAGYGSIPSMAFRMSFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>D5GM78</accession>
    <name>D5GM78_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">C2H2 type master regulator of conidiophore development brlA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00010559001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430352">
        <property type="protein sequence ID" value="CAZ85621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841430.1">
        <property type="nucleotide sequence ID" value="XM_002841384.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GM78"/>
    <dbReference type="GeneID" id="9187405"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010559001"/>
    <dbReference type="HOGENOM" id="CLU_588194_0_0_1"/>
    <dbReference type="InParanoid" id="D5GM78"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR PROTEIN YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003:SF19">
        <property type="entry name" value="YY2 TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="286"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="314"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="139"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="332"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="151"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="167"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="194"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="227"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="361"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="380"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ85621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="465" mass="50121" checksum="A91F2F4AE436D05E" modified="2010-06-15" version="1">MPMARNPDRMDLDVVGDDGQETAWWEHLTKDGVRMREFVEKLNPACLPIDAGKACPEVDNTLEKVYYGLHPSLPPGISIPSFATARAQTISTIIGILRQSHPNTSVSKGDMGAFSISSFLNESYPKSAPFSVEALHSRAGDGVGDGNSVASSHGSSGGPRSSGSLSIRERPSTSDRFHPRDSQPHRHPHHGKPSTHTLSDATGYSQGRSNSDSPGASGSALSDGCPSADESTISSFSSTINTPSRSRTPSHSRSVLNRGPPYVRTGNHFDVPRSRALNGTGPGGQHKCDECEKAFPYPSKLKDHMHIHQQDKPLPCDHLGCLKKFKRARELQAHKKTHDRRATLISRPSQVQFPARSPGQSPVPLPPPQHPFSPLGRGECSTKSKPPDSDRTSSTSPGDEYTFSGSPSPASSHIGGRVATPERPVIDIQGREVIDLKEPDQPTLPASVRKKLDIQLFPNLPGIQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMA5</accession>
    <name>D5GMA5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA ligase</fullName>
            <ecNumber evidence="1">6.5.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00010625001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + (ribonucleotide)n-3'-hydroxyl + 5'-phospho-(ribonucleotide)m = (ribonucleotide)n+m + AMP + diphosphate.</text>
            <dbReference type="EC" id="6.5.1.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRL1 family.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.3" evidence="1"/>
    <dbReference type="EMBL" id="FN430354">
        <property type="protein sequence ID" value="CAZ85648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841457.1">
        <property type="nucleotide sequence ID" value="XM_002841411.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMA5"/>
    <dbReference type="FunCoup" id="D5GMA5">
        <property type="interactions" value="87"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GMA5"/>
    <dbReference type="GeneID" id="9183227"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010625001"/>
    <dbReference type="eggNOG" id="ENOG502QQB9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010316_1_0_1"/>
    <dbReference type="InParanoid" id="D5GMA5"/>
    <dbReference type="OMA" id="FQDWDYK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051730">
        <property type="term" value="F:GTP-dependent polyribonucleotide 5'-hydroxyl-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003972">
        <property type="term" value="F:RNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019039">
        <property type="entry name" value="T4-Rnl1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012387">
        <property type="entry name" value="Trl1_fun"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015966">
        <property type="entry name" value="tRNA_lig_kin_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015965">
        <property type="entry name" value="tRNA_lig_PDEase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32004">
        <property type="entry name" value="TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32004:SF1">
        <property type="entry name" value="TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09511">
        <property type="entry name" value="RNA_lig_T4_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08302">
        <property type="entry name" value="tRNA_lig_CPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08303">
        <property type="entry name" value="tRNA_lig_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019634">
        <property type="entry name" value="tRNA_lig_yeast"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="T4 RNA ligase 1-like N-terminal" evidence="6">
        <location>
            <begin position="71"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA ligase kinase" evidence="5">
        <location>
            <begin position="401"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA ligase phosphodiesterase" evidence="4">
        <location>
            <begin position="563"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="604"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="active site" description="N6-AMP-lysine intermediate" evidence="2">
        <location>
            <position position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR019634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR019634-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF09511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ85648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="823" mass="93083" checksum="FE160F52B1063197" modified="2010-06-15" version="1">MANYSTPFKPQDPDEINGLMELLNRTQAGKKGSARFFCKKKTYQVEGSPIPVDSWKFLDHEYKKKVLPTSARGLFTCKNPTTGDWEIASRGYDKFFNINEVDKTKWTWIEENTNAPYELTVKENGCIIFISGLPDDTLLVCSKHSLGPGLDPDKSHAVFGEKWVDRQLEKLGKSRAEFARALREANVTAVAELCDDTFEEHILPYEDDRAGLYLHGVNLRLPKFATYSAADVKRFARDWGFKVVDSLDKPDVQSLRDFLEETAETGSWDGKDVEGFVIRCKARDGPDGEWHDWFFKYKFEEPYLMYRLWRECTKSMIGGEKPKIKKHKAITTEYLKFAEKYFREHPEAKEKYEENHGIIKLRNAFLEHRGQKGSDIIREEVGEDTESEGADGGNTQKDNVVLASIATLGCGKTTVAQALHELFGWGHVQNDNITGKTGKGRAFATAISNALRKHPVVFADRNNHQFREREQLIKDVSKLNYKATFVAIYYAHPEDLMGEIENTTAARVFGRGDNHQTIRASTQSRRSVEGIMKGFLQRFQGLDPRRLPDSDFNATIELNPQADSRENLEVVVTGLKRHYPNLIPELPTPEDYDRAISAALNYAPSRHTASHNTPRFSQPRGEAKSAVSPGETPSATGPGEAPKQEGKQSVRNPKRKRIDYFGILVPTQEVYAAVNSAFEAQPERIRGFWRELNANDRVQKTFHVTLCHTSSQAKQPEIWKKYMDLLQSKGGELSTILGRGDVELHSMAWDGRVMAVRVLLPDEWECANEAPHITIGTANSSIKPVESNKMLEAWSLGNNDIFSLELQGIKVKGEVKPFGKVDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMC2</accession>
    <name>D5GMC2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 11">RNA cytidine acetyltransferase</fullName>
            <ecNumber evidence="11">2.3.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">18S rRNA cytosine acetyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">NAT10</name>
        <name evidence="17" type="ORF">GSTUM_00010647001</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">RNA cytidine acetyltransferase with specificity toward both 18S rRNA and tRNAs. Catalyzes the formation of N(4)-acetylcytidine (ac4C) in 18S rRNA. Required for early nucleolar cleavages of precursor rRNA at sites A0, A1 and A2 during 18S rRNA synthesis. Catalyzes the formation of ac4C in serine and leucine tRNAs. Requires the tRNA-binding adapter protein TAN1 for full tRNA acetyltransferase activity but not for 18S rRNA acetylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 11">
            <text>a cytidine in 18S rRNA + acetyl-CoA + ATP + H2O = an N(4)-acetylcytidine in 18S rRNA + ADP + phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51424"/>
            <dbReference type="Rhea" id="RHEA-COMP:13575"/>
            <dbReference type="Rhea" id="RHEA-COMP:13576"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:74900"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a cytidine in tRNA + acetyl-CoA + ATP + H2O = an N(4)-acetylcytidine in tRNA + ADP + phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53876"/>
            <dbReference type="Rhea" id="RHEA-COMP:13670"/>
            <dbReference type="Rhea" id="RHEA-COMP:13671"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:74900"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Interacts with TAN1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
            <location evidence="1 11">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the RNA cytidine acetyltransferase family. NAT10 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="11"/>
    <dbReference type="EMBL" id="FN430354">
        <property type="protein sequence ID" value="CAZ85665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841474.1">
        <property type="nucleotide sequence ID" value="XM_002841428.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMC2"/>
    <dbReference type="FunCoup" id="D5GMC2">
        <property type="interactions" value="1078"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GMC2"/>
    <dbReference type="GeneID" id="9183235"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010647001"/>
    <dbReference type="eggNOG" id="KOG2036">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004652_0_0_1"/>
    <dbReference type="InParanoid" id="D5GMC2"/>
    <dbReference type="OMA" id="HLHYIMS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990883">
        <property type="term" value="F:18S rRNA cytidine N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051392">
        <property type="term" value="F:tRNA cytidine N4-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016556">
        <property type="term" value="P:mRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904812">
        <property type="term" value="P:rRNA acetylation involved in maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051391">
        <property type="term" value="P:tRNA acetylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11040:FF:000002">
        <property type="entry name" value="RNA cytidine acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002218">
        <property type="entry name" value="tRNA(Met) cytidine acetyltransferase TmcA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03211">
        <property type="entry name" value="RNA_acetyltr_Nat10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033688">
        <property type="entry name" value="NAT10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007807">
        <property type="entry name" value="TcmA/NAT10_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032672">
        <property type="entry name" value="TmcA/NAT10/Kre33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013562">
        <property type="entry name" value="TmcA/NAT10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027992">
        <property type="entry name" value="tRNA_bind_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10925">
        <property type="entry name" value="N-ACETYLTRANSFERASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10925:SF5">
        <property type="entry name" value="RNA CYTIDINE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13718">
        <property type="entry name" value="GNAT_acetyltr_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05127">
        <property type="entry name" value="NAT10_TcmA_helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08351">
        <property type="entry name" value="TmcA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13725">
        <property type="entry name" value="tRNA_bind_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="3 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 11" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TmcA/NAT10 N-terminal" evidence="14">
        <location>
            <begin position="22"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="TcmA/NAT10 helicase" evidence="13">
        <location>
            <begin position="285"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="15">
        <location>
            <begin position="500"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="domain" description="Possible tRNA binding" evidence="16">
        <location>
            <begin position="697"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="929"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="944"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="981"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="290"/>
            <end position="299"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="449"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="562"/>
            <end position="564"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="569"/>
            <end position="575"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="663"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00052133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00068357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF05127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF13718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF13725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAZ85665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="993" mass="111583" checksum="DF5C473B7F2510F9" modified="2010-06-15" version="1">MVFRYKFFSKSTSVLKVLTVVKEKKRSFFVVVGDRGKDVILNLHYILGSADPKQNKSVLWTYKKDLLGFSSHRKKREAKIKKEVKRGIREANAEDPFELFISLTNIRYCYYKETEKILGNTYGMCIIQDFEALTPNLLARTIETVEGGGLVILLLKTMTSLKQLYTMTMDIHSRYRTEAHGDVVARFNERFILSLADCASCLVVDDELNVLPISGARGVTALPPVSSEEGMTEQKRELLELKESLSEVQPVGSLVDISKTVDQAKAIMTFVDAITEKTLRSTVTLTAGRGRGKSAALGVAIAAAVAHGYSNIFITSPSPENLKTLFEFIFKGFDALNYSDHLDYDIIQSTNPAFNKAIVRVNIHRQHRQTIQYIQPQDAHVLGQAELVVVDEAAAIPLPLVKKLMGPYLVFMASTINGYEGTGRSLSLKLIQQLRDQSLREATLSEPIRYAPNDPVEKWLNNLLCLDATLPSNRSTVHGTPDTAKCELFYVNRDTLFSYHPNIPDPLCVIQVSLEGQISRESVQQSLARGRREGGDMIPWLVSQQFQDEGFASLSGARVVRIATNPDYTGMGYGSRALQLLTDFYEGKFQSLDEGATYVDEGMVRVTDEEIENSTLQTDNIKIRDRESLPPLLLRMSERRPDTLDYIGVSYGLTDKLHKFWKKQKFAPVYLRQTATELTGEHSCVMIKTLASKDNDWLGAFARDFHKRFLTLLSYEFRKFPAVLALTIFESANAGARLSGQEDLPLTKAQLDEYLSPFDLKRLESYADNMLDYHVIVDMIPLIAQLYFTGRLRGSVKLAGVQQAVLMAIGLQRKTLEDIEKELNLPPAQVLSMFQKTIRKVATCFRHLLSQAISETLPQEAGKPKQKKINGGDNNNEIETDEFYPVEQSLADDLQEGGEEVIASMKLKEKQRELINSLDLSKYEIPSTESADWESAEKQIKSATSKNSSGKSTVVSVKTKSHNKRKAGETVTEIYEQEMRKVHKKGKKSKSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMF2</accession>
    <name>D5GMF2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosome biogenesis protein YTM1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">YTM1</name>
        <name evidence="10" type="ORF">GSTUM_00010679001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the NOP7 complex, which is required for maturation of the 25S and 5.8S ribosomal RNAs and formation of the 60S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the NOP7 complex, composed of ERB1, NOP7 and YTM1. Within the NOP7 complex ERB1 appears to interact directly with NOP7 and YTM1. The NOP7 complex also associates with the 66S pre-ribosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
            <location evidence="6">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
            <location evidence="6">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the WD repeat WDR12/YTM1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430354">
        <property type="protein sequence ID" value="CAZ85695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841504.1">
        <property type="nucleotide sequence ID" value="XM_002841458.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMF2"/>
    <dbReference type="FunCoup" id="D5GMF2">
        <property type="interactions" value="858"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GMF2"/>
    <dbReference type="GeneID" id="9182121"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010679001"/>
    <dbReference type="eggNOG" id="KOG0313">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_57_0_1"/>
    <dbReference type="InParanoid" id="D5GMF2"/>
    <dbReference type="OMA" id="DIQAPDW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070545">
        <property type="term" value="C:PeBoW complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043021">
        <property type="term" value="F:ribonucleoprotein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051276">
        <property type="term" value="P:chromosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110136">
        <property type="term" value="P:protein-RNA complex remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03029">
        <property type="entry name" value="WDR12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012972">
        <property type="entry name" value="NLE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028599">
        <property type="entry name" value="WDR12/Ytm1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19855:SF11">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN WDR12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19855">
        <property type="entry name" value="WD40 REPEAT PROTEIN 12, 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08154">
        <property type="entry name" value="NLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 6" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="2 6" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="3 7" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="NLE" evidence="9">
        <location>
            <begin position="20"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="109"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="150"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="199"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="268"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="355"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="238"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ85695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="441" mass="47850" checksum="CB69B7FF72A6ECEA" modified="2010-06-15" version="1">MTSAEDSPMGGTDPQSTHQIQVKFTTRHPEIAVPTAPILVPANLKRYGLSQIINHLLDTSTATPFEFLINGSFLRTSIEKYLSDNGLSSESVLTLEYVRAILPPKFQAAFQHDDWVSSVSVEGENRRILSGSYDGIVRVWNESAQCLYTGVGHTDAVKTTKWMSATKIVSAGMDRGLRIWEVDGEYIGEISEVSALAEFVGHRSTVEKISVDSSSGRVLSAGMDSLVGLWTTNISEAPKAPKEAPVPKAKRRKESSAGPPRYGSLRMLEGHTSPVSGVAFDPKDSTVGYSVSWDHTIRTWDLATCSLADSRTTQHPLLSVCVLPTLSLLACGSSARHITLHDPRVSASTVTTSTLRGHTNAVVALSPNKNSEWQMVSASHDGTLRIWDVRATESGSIWTIKREGEGLSGKEKVFDVEWSSLGIVSGGEDKRVQIDESPKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNZ8</accession>
    <name>D5GNZ8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00011601001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the TPP enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430371">
        <property type="protein sequence ID" value="CAZ86241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842050.1">
        <property type="nucleotide sequence ID" value="XM_002842004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNZ8"/>
    <dbReference type="STRING" id="656061.D5GNZ8"/>
    <dbReference type="GeneID" id="9183534"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011601001"/>
    <dbReference type="eggNOG" id="KOG1185">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013748_4_0_1"/>
    <dbReference type="InParanoid" id="D5GNZ8"/>
    <dbReference type="OMA" id="DMCFPGD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005948">
        <property type="term" value="C:acetolactate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07035">
        <property type="entry name" value="TPP_PYR_POX_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045229">
        <property type="entry name" value="TPP_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006203">
        <property type="entry name" value="PRK08327.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968:SF164">
        <property type="entry name" value="PYRUVATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968">
        <property type="entry name" value="THIAMINE PYROPHOSPHATE ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="6">
        <location>
            <begin position="27"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme central" evidence="4">
        <location>
            <begin position="233"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="5">
        <location>
            <begin position="439"/>
            <end position="597"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ86241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="600" mass="64882" checksum="C924248F822870A3" modified="2010-06-15" version="1">MTPFTLSSSLSRLPPQNQLLKLSMYTASFAFFEALWEAGVTHCFVNLGSDHPSILEAIVKGKRERPHSFPEFLTCPNEMVALSAAHGMAQVTGKPQAVIVHVDVGTQGLGAAVHNASCSRVPVLIFAGLSPFTQEGEMRGSRTEFIHYIQDVPDQKAIVAQYCRYSAEIKTGKNIKQMVNRAISFAMSDPKGPVYLTAAREVLEEDIEPYSLNQAVWDPISPTALTEDAVGRISQSLLSAENPLVITGYSGRNKYAVEELITLADSVPGLRVLDTLGSDMCFPANHRSSLGVMYGSHEKITTADVILVIDCDVPWIPTQCRPSPRAAVYHIDIDPLKQQMPLFYIDADARYRADAYTALKQINSYTAVMKMDAATRDARIQALDNEHKTRMDRLEQQAKPRDDGLLSCAHVMKVLRGAIPQDSVIILEAVTQTVTVVDQMKCSLPKTIFNSGAGGLGWFGGAALGAKLALDNGTSNSGPFVVAIVGDGTFLFSVPASVYWISRRYKIPFLTIVINNDGWNAPLRSAKLVHPDGLSAHATPEELNISFKPSPDYAGIAVAAAGGEIWGGKAEKVEQLASALEEAIGVVKGGRSAVVEVVIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPA8</accession>
    <name>D5GPA8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00011781001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430373">
        <property type="protein sequence ID" value="CAZ86373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842182.1">
        <property type="nucleotide sequence ID" value="XM_002842136.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPA8"/>
    <dbReference type="GeneID" id="9186613"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011781001"/>
    <dbReference type="HOGENOM" id="CLU_3368802_0_0_1"/>
    <dbReference type="InParanoid" id="D5GPA8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072502" evidence="1">
        <location>
            <begin position="17"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ86373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="35" mass="4076" checksum="D297B7BDEDE08ED7" modified="2010-06-15" version="1">MVWLFCFLSYSYLILSRVSQQFSSSSSLPSTSFLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPI0</accession>
    <name>D5GPI0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Coatomer subunit zeta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00011852001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. The zeta subunit may be involved in regulating the coat assembly and, hence, the rate of biosynthetic protein transport due to its association-dissociation properties with the coatomer complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 12">Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 12">Golgi apparatus membrane</location>
            <topology evidence="1 12">Peripheral membrane protein</topology>
            <orientation evidence="1 12">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Cytoplasmic vesicle</location>
            <location evidence="12">COPI-coated vesicle membrane</location>
            <topology evidence="12">Peripheral membrane protein</topology>
            <orientation evidence="12">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the adaptor complexes small subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430375">
        <property type="protein sequence ID" value="CAZ86423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842232.1">
        <property type="nucleotide sequence ID" value="XM_002842186.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPI0"/>
    <dbReference type="FunCoup" id="D5GPI0">
        <property type="interactions" value="476"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GPI0"/>
    <dbReference type="GeneID" id="9186663"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011852001"/>
    <dbReference type="eggNOG" id="KOG3343">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086803_0_0_1"/>
    <dbReference type="InParanoid" id="D5GPI0"/>
    <dbReference type="OMA" id="NELMLHS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14829">
        <property type="entry name" value="Zeta-COP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000013">
        <property type="entry name" value="Coatomer subunit zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039652">
        <property type="entry name" value="Coatomer_zeta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11043:SF0">
        <property type="entry name" value="COATOMER SUBUNIT ZETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11043">
        <property type="entry name" value="ZETA-COAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 12" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6 12" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8 12" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AP complex mu/sigma subunit" evidence="13">
        <location>
            <begin position="10"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU366053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ86423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="198" mass="21741" checksum="755FB7835E9FA2B7" modified="2010-06-15" version="1">MAPNLSLFSVNAILILSPDGTRVLAKYYHPPHSPATAANNAAASNPYPTLKEQKPFEKGLVEKTAKSNSDIILYDSRIVVFKAESDVMLYVIGGVEENEMLLWHTLLALRDSLNILLKNATDKRTLLENYDIVSLAIDEIVDDGIILETDPATIAGRVSKPPMQEVGGVKGLDLSEQGLLNAWEFGKKQLAERLRQGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPK2</accession>
    <name>D5GPK2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00011882001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the isochorismatase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430375">
        <property type="protein sequence ID" value="CAZ86445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842254.1">
        <property type="nucleotide sequence ID" value="XM_002842208.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPK2"/>
    <dbReference type="STRING" id="656061.D5GPK2"/>
    <dbReference type="GeneID" id="9186685"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011882001"/>
    <dbReference type="eggNOG" id="ENOG502QRZN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005335_0_0_1"/>
    <dbReference type="InParanoid" id="D5GPK2"/>
    <dbReference type="OMA" id="KMYHLSG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006307">
        <property type="term" value="P:DNA alkylation repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00431">
        <property type="entry name" value="cysteine_hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00299">
        <property type="entry name" value="GST_C_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00570">
        <property type="entry name" value="GST_N_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.590">
        <property type="entry name" value="Alpha-ketoglutarate-dependent dioxygenase AlkB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.850">
        <property type="entry name" value="Isochorismatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027450">
        <property type="entry name" value="AlkB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037151">
        <property type="entry name" value="AlkB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032854">
        <property type="entry name" value="ALKBH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000868">
        <property type="entry name" value="Isochorismatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036380">
        <property type="entry name" value="Isochorismatase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31212">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT DIOXYGENASE ALKB HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31212:SF5">
        <property type="entry name" value="ISOCHORISMATASE FAMILY PROTEIN FAMILY (AFU_ORTHOLOGUE AFUA_3G14500)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13532">
        <property type="entry name" value="2OG-FeII_Oxy_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13417">
        <property type="entry name" value="GST_N_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00857">
        <property type="entry name" value="Isochorismatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52499">
        <property type="entry name" value="Isochorismatase-like hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="5">
        <location>
            <begin position="503"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="domain" description="GST N-terminal" evidence="3">
        <location>
            <begin position="707"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="4">
        <location>
            <begin position="774"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="84"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="274"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="328"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="549"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="84"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="119"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="305"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="340"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="389"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="550"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="575"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ86445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="902" mass="100619" checksum="D58E882268615B82" modified="2010-06-15" version="1">MGPRGALLLVGLQNEFLDPHMGRCLMTPPEPGKPSFVDNIKNFLPHFRARGGDVIWVRSEYRKPRDCEDPRNDEVVLLVDDEGYYVDEEEQEPLPEANTQEGARRKGKSATTLKHDGQLSPSTAKRSNTTAEENYVGPPQLHTDAFLAIESDNPPCAPSSIESEFYPPLLPVIQTPPDRVLIKTWFSAFKDTKLLETLRGRFITELYLCGTIANACILATAADAAKHGLEVTILTDCVGYRSEMARDRAMRVMIDDFAVEEVRSTLLAKSWEKTRNKSKGATGPAHAGVSGKVEKLLEVDTESSGYERGDYDYERTSELGELRLDSLSLKDGDAPQPPDSGESAGAAVEGGAESPVPTSPSPTSAKPQSPIQDKNPIERAPATREPVLRKPRMASRNKKFQSKAPVLGQGDVLGEGDSEVVNNILPADLADVAFERLKKEVAWRSMYHRGGEVPRLVAVEGEISDDGSFPIYRHPADESPPLLPFSDTVGLIREYVQRVLKQPVNHVLIQHYRDGNDYISEHSDKTLDIAKGSKIVNVSLGAQRVMILRTKKDQGKPDESSGAETNPDKCKTKESAPQSSQRPTQRIPLPHNSMFVLGLESNKKWLHGIKQDKRAEFLKTPKELSYNGERISLTFRQIATFLSSDEKTIYGQGATSKSEHEPREVINGEGPEAEKLLEAFGSENHQSDFDWDTYYGAGSDVLHLKEQVPKLFFVRNDPGGLRVKICLHEKGIRFVDHELKITEAQDETQTPSFLTLSPHGHTPVFIDIDKEKTTVCESLAIIQYLEMFYPSKEDKWLLPSLTDERAAFARVLQRMQESEQLATVLKHSHDEGAVEREFAIWEEYLSKAGGQIAGDEFSLADIAAFPVIDQFVKRTGYKIAKWREIEAWLTNMGYRESVRATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPK8</accession>
    <name>D5GPK8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00011894001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430376">
        <property type="protein sequence ID" value="CAZ86451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842260.1">
        <property type="nucleotide sequence ID" value="XM_002842214.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPK8"/>
    <dbReference type="FunCoup" id="D5GPK8">
        <property type="interactions" value="940"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GPK8"/>
    <dbReference type="GeneID" id="9186691"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011894001"/>
    <dbReference type="eggNOG" id="KOG0710">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046737_1_1_1"/>
    <dbReference type="InParanoid" id="D5GPK8"/>
    <dbReference type="OMA" id="DICETPQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06464">
        <property type="entry name" value="ACD_sHsps-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031107">
        <property type="entry name" value="Small_HSP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11527">
        <property type="entry name" value="HEAT-SHOCK PROTEIN 20 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="5">
        <location>
            <begin position="25"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="74"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="88"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ86451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="161" mass="18353" checksum="D193AD6F6770270E" modified="2010-06-15" version="1">MSLLRQYSDIFSLLDDTSRIPQRTNRRGAFSPSFDVHETEQAYVLEGELPGLHDKSNLSIEFTDPQTLLVRGKTERLHQESSDNEPAIAKKDKKEGAIEEKDKDSRPRYWVSERTVGEFQRSFSFPSYVDMDAVKASLAHGILKIVVPKKQSQIAKKIEIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>D5GQ49</accession>
    <name>D5GQ49_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00012198001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430382">
        <property type="protein sequence ID" value="CAZ86642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842451.1">
        <property type="nucleotide sequence ID" value="XM_002842405.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GQ49"/>
    <dbReference type="STRING" id="656061.D5GQ49"/>
    <dbReference type="GeneID" id="9185222"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012198001"/>
    <dbReference type="eggNOG" id="KOG0015">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063931_1_0_1"/>
    <dbReference type="InParanoid" id="D5GQ49"/>
    <dbReference type="OMA" id="GGHYPQD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000987">
        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00266">
        <property type="entry name" value="MADS_SRF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1810.10:FF:000002">
        <property type="entry name" value="Serum response factor b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050142">
        <property type="entry name" value="MADS-box/MEF2_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033897">
        <property type="entry name" value="SRF-like_MADS-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48019">
        <property type="entry name" value="SERUM RESPONSE FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00350">
        <property type="entry name" value="MADS_BOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="7">
        <location>
            <begin position="66"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="146"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="197"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ86642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="248" mass="26605" checksum="9289FEDEF1B7A429" modified="2010-06-15" version="1">MSDNDPQPSPGPHDDSAGPNASGLAGNGGVPNSNPNASGVDPSRGVKRTRPNQDDDDDDEDNKPGRERRKIEIKFIQDKSRRHITFSKRKAGIMKKAYELSVLTGTQVLLLVVSETGLVYTFTTPKLQPLVTKAEGKNLIQACLNAPEPHSNENGVAEGDSTVDSPEEPQHTQLPTQVSGRPPVGMPVGPGVPPGPYMNMPDAATQAQAHAYQQYMQQQQAAAQVQAQRGGYLPQHPAQMQPHPAQTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5G496</accession>
    <name>D5G496_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00004011001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835218.1">
        <property type="nucleotide sequence ID" value="XM_002835172.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G496"/>
    <dbReference type="GeneID" id="9184964"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004011001"/>
    <dbReference type="HOGENOM" id="CLU_2335164_0_0_1"/>
    <dbReference type="InParanoid" id="D5G496"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ79339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="98" mass="10751" checksum="4F9C542971B37DAB" modified="2010-06-15" version="1">MSQAGSKLSWVVLGCCNKHWSAATHPAIGCVISSFTSTAALFPRPPEDKACSILQALRQYFSPVPGRFCWTLTKVKAHTYIPEVIPLFSSPELFPLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4M6</accession>
    <name>D5G4M6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00000014001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the phosphatase 2A regulatory subunit A family.</text>
    </comment>
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        <property type="protein sequence ID" value="CAZ79469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="eggNOG" id="KOG0211">
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:establishment or maintenance of cytoskeleton polarity"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990813">
        <property type="term" value="P:meiotic centromeric cohesion protection in anaphase I"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031030">
        <property type="term" value="P:negative regulation of septation initiation signaling"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="entry name" value="Serine/threonine-protein phosphatase 2A regulatory subunit A alpha isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054573">
        <property type="entry name" value="PP2A/SF3B1-like_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051023">
        <property type="entry name" value="PP2A_Regulatory_Subunit_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10648:SF4">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2 (FORMERLY 2A), REGULATORY SUBUNIT A, BETA ISOFORM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10648">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A 65 KDA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13646">
        <property type="entry name" value="HEAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22646">
        <property type="entry name" value="PPP2R1A-like_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="12"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="89"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
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            <end position="204"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="205"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="246"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatase PP2A regulatory subunit A/Splicing factor 3B subunit 1-like HEAT repeat" evidence="4">
        <location>
            <begin position="279"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="285"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="324"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="363"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="402"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="519"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="3">
        <location>
            <begin position="581"/>
            <end position="614"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00038332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF22646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ79469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="614" mass="68482" checksum="F93B4E979F2D2738" modified="2010-06-15" version="1">MDIQPSNDELYPIAVLIDELKHDDVLLRLNAIKRLSTIALALGPERTRDELIPFLDESVEDEDEVLTALSEVLGSFVEYVGGQEHGHVLLSPLENLAAIEEPLVREKAVDSLNKICQELSQNQVEEYFIPLVIRLSKADWFTSKISATGLYTVPYSKATPRSQDGLRQHFAQLVHDDTPMVRRQAATNLAKFAKTMPADLVVSEMIPLFQHLAQDDQDSVRLLTVEVLISIAEVVPKDQQASHVVILTILRSLFGDKSWRVRYMVADKFEKIAKAVESDVVRRDLVPAFVKLLKDSEAEVRTAIAGQIPGFCKLLDRQILLDEIMSPVEDLVTDTSQHVRAALGTQISGLAPILGKDETISHLLPMFLQMLKDEFPDVRLHIISKLEQVNDVIGIELLSQSLLPAIVQLAEDKQWRVRLAIIEYIPLLAKQLGVEFFDEKLGNLCMSWLGDTVFSIRQAATLNLKQLTDVFGVEWARQTIIPKVMAMGDQRNYLYRMTTCAAITTLAPAVNVAAIEEFILPMIHKLIRDPIPNIRFNVAKSYGVLIDVLRKLPAEGTLANVKPDSAAGPSDLGLELIQKQIVPNLEKLQADSDVDVRFFAAQAMANIGDLMQTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4P3</accession>
    <name>D5G4P3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00000089001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429990">
        <property type="protein sequence ID" value="CAZ79519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835362.1">
        <property type="nucleotide sequence ID" value="XM_002835316.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4P3"/>
    <dbReference type="STRING" id="656061.D5G4P3"/>
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    <dbReference type="KEGG" id="tml:GSTUM_00000089001"/>
    <dbReference type="eggNOG" id="KOG0126">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045495_2_1_1"/>
    <dbReference type="InParanoid" id="D5G4P3"/>
    <dbReference type="OMA" id="GSWHVDY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12411">
        <property type="entry name" value="RRM_ist3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
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        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051847">
        <property type="entry name" value="RNA_proc/Spliceosome_comp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045844">
        <property type="entry name" value="RRM_Ist3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45880">
        <property type="entry name" value="RNA-BINDING MOTIF PROTEIN, X-LINKED 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45880:SF1">
        <property type="entry name" value="RNA-BINDING MOTIF PROTEIN, X-LINKED 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="33"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="114"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="151"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="198"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="217"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="239"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ79519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="322" mass="37745" checksum="C279B16861C059FE" modified="2010-06-15" version="1">MNNIRAIEKLNQRELEAGIPLSASWHTDWSDTAYVYIGGLPYELSEGDIITIFSQFGEPVHVNLIRDKDTGKSKGFAFLKYEDQRSTNLAVDNLGGATILGRIIRVDHTRYKPKEGELEELLGQGSGPRDRDDGDDDDESEHHSKRRRGKGREDMPPREMLPEEIELQKLIRDHDEDDPMKEYLVRQKKEQVELALQKLKREKKSSHHRRDGDSKRDHRHSHRHRSRSEGRSRRHRSRSKEQTHKERASEEPSRSQERGRDGRRGKYHDRDGGRDKGKDRDRYGDWDGDRGGSSRLEKERGRDRFHRDSGHSSRREGRRYDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4Q2</accession>
    <name>D5G4Q2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00000038001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429990">
        <property type="protein sequence ID" value="CAZ79488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835331.1">
        <property type="nucleotide sequence ID" value="XM_002835285.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4Q2"/>
    <dbReference type="STRING" id="656061.D5G4Q2"/>
    <dbReference type="GeneID" id="9188424"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000038001"/>
    <dbReference type="eggNOG" id="KOG1153">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1918593_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4Q2"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ79488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="132" mass="14110" checksum="2B890E0D54A6DF19" modified="2010-06-15" version="1">MASPHIAGLLAYFISLQPKQGSSYAVGELTPAQLKKNMVSIASEGCLTGIPSDTPNLLAYNGGGSGNLSDIFDGTRYKHGHKSSNGVVEDVTDVTKELINETEAFLDDIEDKAVHEIKTLFDKARAALNSRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4T7</accession>
    <name>D5G4T7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00000194001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429991">
        <property type="protein sequence ID" value="CAZ79530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835373.1">
        <property type="nucleotide sequence ID" value="XM_002835327.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4T7"/>
    <dbReference type="GeneID" id="9183012"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000194001"/>
    <dbReference type="eggNOG" id="ENOG502SD68">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019095_0_2_1"/>
    <dbReference type="InParanoid" id="D5G4T7"/>
    <dbReference type="OMA" id="GHSWIDC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055915">
        <property type="entry name" value="DUF7492"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24320">
        <property type="entry name" value="DUF7492"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003071973" evidence="2">
        <location>
            <begin position="26"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7492" evidence="3">
        <location>
            <begin position="24"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ79530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="267" mass="28615" checksum="B14BAC3E14646D4E" modified="2010-06-15" version="1">MYGPTINLFQLAGVAAMTLISGAHGHSWIDGLKVVSGGNSGPFQNTTTTKEGPQGAGIGFIRAYQGHVDATAVYLITTPKLDSNICRPSQQQNPTTNTPKFPRVSASPGDFIQGTYLENGHISKPDAADGTPGTIYWFGTANAKPDDTLANVKQWTVDGKGGDGRGRLLANPSNFDDGKCAETNDTPIAKQRKAAGGGGACKSVFKLPEDAKVGSTFTVYWVWDYSNHFGPTKKGHTEWYTSCIDIQVVAPKGKRFWRGSAKFRARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5D6</accession>
    <name>D5G5D6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00004283001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835572.1">
        <property type="nucleotide sequence ID" value="XM_002835526.1"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G5D6"/>
    <dbReference type="GeneID" id="9188148"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004283001"/>
    <dbReference type="eggNOG" id="KOG3630">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003852_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5D6"/>
    <dbReference type="OMA" id="PIMYAYL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039462">
        <property type="entry name" value="Nup159/Nup146_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16755">
        <property type="entry name" value="Beta-prop_NUP159_NUP214"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117289">
        <property type="entry name" value="Nucleoporin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nucleoporin Nup159/Nup146 N-terminal" evidence="6">
        <location>
            <begin position="10"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="271"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="370"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="519"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="950"/>
            <end position="1175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1416"/>
            <end position="1464"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1338"/>
            <end position="1372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="272"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="303"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="339"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="371"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="532"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="565"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="607"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="650"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="703"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="738"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="750"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="804"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="825"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="842"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="859"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="916"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1031"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1049"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1078"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1125"/>
            <end position="1140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1154"/>
            <end position="1172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1418"/>
            <end position="1437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1440"/>
            <end position="1452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1453"/>
            <end position="1462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF16755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ79729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1514" mass="159548" checksum="8ADBD1303AEB87EB" modified="2010-06-15" version="1">MVNIDTRGFEKSSSGDVVLKKEASTVSWSPLGKQIVCGMGDGTGWQMTPGGEVKAVLPSVPGLENYFISSIVWVGNATFITTHTPVPSDDANNESVFHALVRDGTSYQYTKLPDPIPPFGMDSRTPPYFFHAMIRQYAPGMRDMIMFTNTCSSDVGLVCRFSTALTGDPSVPADNWITAGIGDDHRRAQIPLGGESFSDTSPIGMALDLSNKANVKRPVGGEEIEESPGPLPILMVLNHEGLLSAWNVIYNDAIEQNEKYNGMMVYVTQEGPSQSAPQTPQQPASQRQSVQNITPSPAFGVNSFGQSPVSNGNGTFGQPSSLGSPNTFAKPATGAFGQSAASPTFGQPSSIGASGASAFGKPSALGGSMWGSTSGASPSAPAPTSATAFGQPSALGTRSVFGQPATLGGQPAFGRTAALGVQPVFGQPAPLGSATTAPAFGAPTLIGANKPGTAGFSAVSSLGSPPAGGSPFGSTSAFGGGSAFGAYANKGGFSAAAAAAGSGDSKPIWGTGKSLGSDTTSTMFGGGDNVPSPFTSGSTGSFKLSSAFKPDPESAPENKPFPPSGGFGSLGVGLGDALTSTPSAISRPGPLPTPGGVSDQNIRDASMDDSDEQPDEPSDDDDIISPEATEARQAGFPSTPAKPGLPHGIFSSTKAAGSSSSASNPFTSANQSSSPFSSVPGPSSGSPFGAWGAQRKTPFGVPSTPPKSPFPTTPKLSSPFPGPSRPLTFAQSPEKSPESAQAAVAAPVTSSPPPPSLPPHSPFTSEEKGLETLPALPADESSTPALTEEASSIPLPSGPESVKESSGTQASESPSLPSPGPKSVGTDNEQVTSLDGSDDDNDGHQESKGEGDEGKRDNGDEDEDEDGDDEDGDDEDGGDEDGDDEDEDDEDEGNSEGDDSPLSEGFVFEPKPKLPSASSGPSTSDVFGTDTTFSGTFIKGGIEENALFSPLAKTPLPVNDSDSDRSRNSLLASPNLKNRSPGAAVGSLANKNKGGKNSLGRRGVSNRPPKKPDASKILSTLCPSDFLASRNKPQGTFPFQPSADDSINKPAGSSPLAAPALQAKQASQEEGEKEVQEEERVQEEGEEEFRGEREAQGEEKVQEEGDEGPQKEKKAQEEEGAQEEGVQKEKKAQEEERVQEEGEEEVQEEKKAQEEEEVESDLDFDNEYDDYDPDRVRNKLLNSDVKPVQELPKFRRNGEVEVASDFNAVFDSIYLSGNMMINRLGLTVQGLKAYHLGQENPLPDVEHKTAKEMRHPLDWRFCEMDQVKEILEQLLASVKGENQGNDELEARYQAASKGFIKLDVKDAEIKRLLAVHNDAQAAAIARARPLTPEQVLQQRKLRKEYAKVERLLKEAEKEVTQLKAKIASRDRDRPGAVVPPTVEAVKNTIMKLTGMVERKNGDIDYLEERLRRVKIKSRSASATPGKSRGLTPNQTPRGETPQRFETPEKLRSYDNNTSSVSYPASPVLALADPLDVEEAREEIRARKEMGRKMRDALEICGPKLTTVSRWSGEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5E8</accession>
    <name>D5G5E8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">GSTUM_00004300001</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">ATP-dependent RNA helicase involved in 40S ribosomal subunit biogenesis. Required for the processing and cleavage of 35S pre-rRNA at sites A0, A1, and A2, leading to mature 18S rRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the DEAD box helicase family. DDX48/FAL1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835584.1">
        <property type="nucleotide sequence ID" value="XM_002835538.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5E8"/>
    <dbReference type="FunCoup" id="D5G5E8">
        <property type="interactions" value="627"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G5E8"/>
    <dbReference type="GeneID" id="9188160"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004300001"/>
    <dbReference type="eggNOG" id="KOG0328">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_1_0_1"/>
    <dbReference type="InParanoid" id="D5G5E8"/>
    <dbReference type="OMA" id="TRFHDFK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18045">
        <property type="entry name" value="DEADc_EIF4AIII_DDX48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000031">
        <property type="entry name" value="Eukaryotic initiation factor 4A-III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000498">
        <property type="entry name" value="Eukaryotic initiation factor 4A-III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 15" id="KW-0347">Helicase</keyword>
    <keyword evidence="6 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="18">
        <location>
            <begin position="25"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="16">
        <location>
            <begin position="56"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="17">
        <location>
            <begin position="237"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="14">
        <location>
            <begin position="25"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAZ79741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="388" mass="44043" checksum="EB4FEEDBAC34E514" modified="2010-06-15" version="1">MAEGIDRREDARLEFSTSKEVTVAPTFEAMHLKEGLLRGIYAYGYESPSAVQSRAIVQICKGRDTIAQAQSGTGKTATFSISILQVIDTAVRETQALVLSPTRELATQIQSVVMALGDYMNVQCHACIGGTNVGEDIRKLDYGQHVVSGTPGRVADMIRRRNLRTKHIKMLVLDEADELLNRGFREQIYDVYRYLPPSTQVVVVSATLPYDVLDMTTKFMTDPVRILVKRDELTLEGLKQYFIAVEKEEWKFDTLCDLYDTLTITQAVIFCNTRRKVDWLTEKMREANFTVSAMHGEMPQKERDAIMQEFRQGNSRVLISTDVWARGIDVQQVSLNYIHRIGRSGRFGRKGVAINFVTSEDVRILRDIELFYSTQIDEMPMNVSDLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5P4</accession>
    <name>D5G5P4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein farnesyltransferase/geranylgeranyltransferase type-1 subunit alpha</fullName>
            <ecNumber evidence="4">2.5.1.58</ecNumber>
            <ecNumber evidence="3">2.5.1.59</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">CAAX farnesyltransferase subunit alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">FTase-alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Ras proteins prenyltransferase subunit alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Type I protein geranyl-geranyltransferase subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00001514001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein prenyltransferase subunit alpha family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.58" evidence="4"/>
    <dbReference type="EC" id="2.5.1.59" evidence="3"/>
    <dbReference type="EMBL" id="FN430000">
        <property type="protein sequence ID" value="CAZ79837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835680.1">
        <property type="nucleotide sequence ID" value="XM_002835634.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5P4"/>
    <dbReference type="FunCoup" id="D5G5P4">
        <property type="interactions" value="168"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G5P4"/>
    <dbReference type="GeneID" id="9187514"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001514001"/>
    <dbReference type="eggNOG" id="KOG0530">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026582_1_0_1"/>
    <dbReference type="InParanoid" id="D5G5P4"/>
    <dbReference type="OMA" id="WAIRTFN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005953">
        <property type="term" value="C:CAAX-protein geranylgeranyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005965">
        <property type="term" value="C:protein farnesyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004662">
        <property type="term" value="F:CAAX-protein geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004660">
        <property type="term" value="F:protein farnesyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.120">
        <property type="entry name" value="Protein prenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002088">
        <property type="entry name" value="Prenyl_trans_a"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11129">
        <property type="entry name" value="PROTEIN FARNESYLTRANSFERASE ALPHA SUBUNIT/RAB GERANYLGERANYL TRANSFERASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11129:SF1">
        <property type="entry name" value="PROTEIN FARNESYLTRANSFERASE_GERANYLGERANYLTRANSFERASE TYPE-1 SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01239">
        <property type="entry name" value="PPTA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48439">
        <property type="entry name" value="Protein prenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51147">
        <property type="entry name" value="PFTA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0637">Prenyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00043086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00043219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ79837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="329" mass="37708" checksum="FA1AA1C2F786C775" modified="2010-06-15" version="1">MNPTPLYSYASDPAWADLLPLPQDDGQYPLAQIAYTEEYTEAMSYLRAIMAKNEHSPRVLQVTAHIIEMNPAHYTIWVYRAKTLFALSATGEVQLGRELEFLNDLALRHQKNYQIWNHRQTVVEAIAAAAAPAEQEQLVREEKEFMNRMFEQDGKNYHVWSYRQWLVRRFEAWDGELSFVSALLERDVRNNSAWNHRFFVVFGGGGMDARQQGEGEVVEEVVEREIAFIEDAVFIAPQNASAWNYLRGVLKKVHRPLSSVESMCLGYAPLDNPDKITSSHALDLLADIYAEKREEHGKAVKALELLAAKFDPIRANYWNYRVGLVKASV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5P7</accession>
    <name>D5G5P7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00001424001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430001">
        <property type="protein sequence ID" value="CAZ79840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835683.1">
        <property type="nucleotide sequence ID" value="XM_002835637.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5P7"/>
    <dbReference type="STRING" id="656061.D5G5P7"/>
    <dbReference type="GeneID" id="9187518"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001424001"/>
    <dbReference type="eggNOG" id="KOG0198">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_412322_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5P7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00180">
        <property type="entry name" value="PKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF288">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="169"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="448"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="562"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="449"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="464"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="483"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="516"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="566"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="601"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="656"/>
            <end position="666"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ79840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="666" mass="73101" checksum="5EF0FBDFCFD70A89" modified="2010-06-15" version="1">MFAPRPELSILPPAPPAPTDSVGGLSATDDDVDGDVVRSPVDDELDWKWILNLSMHFRDQSDREKFFVTYAETPTLRRRLTVSCDYRDAEEGTLERELATLKYQRDKSARIYDAIRDSLSDIQFYDTITNLKLQTSDGRLHVHVTEDLHEIIPYPPVVAIAHLRCPKYRESELEFDSHLSGFVYKVKVNGDVFIKKEIPGPDTVDEFLYEINALYALADSENVIRFGGCVVDEDRKKVKGLLISFASKGALIDIIYHERGRLSWETREKWAKQIVSGLSEVHEAGYVQGDFTLSNIVIDENGDAKIIDINRRGCPVGWEPPEVTAMIDGLQRIGMYIGVKSDIFQLGMVLWGIAHEMDEPEAVKRPLGMGEAPGDVPDYFRDIVEACLSERPQGRPSAKDLISLFPERDQPSRRYIGEMPHYEGGSGSFAALGGGGYFPNGYPMGLGRPYDEAPREDGGQSMSGVGGNGGVDGTNGPLLNGKLSRNSNSTYTPFLPQELSNHMTGSRRPIVDEDTQSNASNSNRPISRDSFEDAPQVIEAAPSEASGEESYVPNNIESVIGLSPASLPPPPPPPPFVRPPLERYSTANDYFGDELGWPSYMPAPQPRRLPSPPSWGSGGARGMPTNEDYVGRDPQGVESPGNSPTSPTSPPPLSLPIPTTGPGLAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6D9</accession>
    <name>D5G6D9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DNA polymerase</fullName>
            <ecNumber evidence="12">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">GSTUM_00004441001</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="12"/>
    <dbReference type="EMBL" id="FN430009">
        <property type="protein sequence ID" value="CAZ80082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835925.1">
        <property type="nucleotide sequence ID" value="XM_002835879.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5G6D9">
        <property type="interactions" value="899"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G6D9"/>
    <dbReference type="GeneID" id="9181167"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004441001"/>
    <dbReference type="eggNOG" id="KOG0970">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001718_1_0_1"/>
    <dbReference type="InParanoid" id="D5G6D9"/>
    <dbReference type="OMA" id="MTKMNVG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005658">
        <property type="term" value="C:alpha DNA polymerase:primase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006273">
        <property type="term" value="P:lagging strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902975">
        <property type="term" value="P:mitotic DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05776">
        <property type="entry name" value="DNA_polB_alpha_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05532">
        <property type="entry name" value="POLBc_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.60:FF:000004">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000036">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2820:FF:000001">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3200.20">
        <property type="entry name" value="DNA Polymerase alpha, zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="entry name" value="DNA polymerase family B, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017964">
        <property type="entry name" value="DNA-dir_DNA_pol_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006133">
        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006134">
        <property type="entry name" value="DNA-dir_DNA_pol_B_multi_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024647">
        <property type="entry name" value="DNA_pol_a_cat_su_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038256">
        <property type="entry name" value="Pol_alpha_znc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045846">
        <property type="entry name" value="POLBc_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015088">
        <property type="entry name" value="Znf_DNA-dir_DNA_pol_B_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00592">
        <property type="entry name" value="pol2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45861">
        <property type="entry name" value="DNA POLYMERASE ALPHA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45861:SF1">
        <property type="entry name" value="DNA POLYMERASE ALPHA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12254">
        <property type="entry name" value="DNA_pol_alpha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00136">
        <property type="entry name" value="DNA_pol_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08996">
        <property type="entry name" value="zf-DNA_Pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00106">
        <property type="entry name" value="DNAPOLB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00116">
        <property type="entry name" value="DNA_POLYMERASE_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0235">DNA replication</keyword>
    <keyword evidence="10 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 12" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 12" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNA polymerase alpha catalytic subunit N-terminal" evidence="17">
        <location>
            <begin position="18"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B exonuclease" evidence="15">
        <location>
            <begin position="479"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B multifunctional" evidence="14">
        <location>
            <begin position="788"/>
            <end position="1220"/>
        </location>
    </feature>
    <feature type="domain" description="Zinc finger DNA-directed DNA polymerase family B alpha" evidence="16">
        <location>
            <begin position="1257"/>
            <end position="1445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="58"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="109"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="212"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="223"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF03104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF12254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAZ80082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1451" mass="163295" checksum="411B3B36A27BB427" modified="2010-06-15" version="1">MERSRRAGAKKTPHEGFKELRELKASGKTRLSGYKVEEEEDFYDLVDEEEYKKVVRQRLDQDDFVVDDQGEGYADNGMDDWDDDHRREGSWEESEDERSKKGGRSAKARKAEEQKRKGEQEGNIRKFFNSNTAPTAKPKPKVKEENLNFLNDLLGEFDNDSPAEPISLMSKRPRGDPVAKVRRLSPPLKDSKPTRMDQISQAGGEGEKPSFAGSSLSNAGNADNDDQDYGSFDEDVDMGDAPVVPSSPIADAVKRKRSKDEDEDDEDLSVARIQGSKSIKVEAVNIIASRPALKSQTVKQEAVVPNKPVAAIDPTTWASFAGGDNVTSSPALDGSDLEKLGPEDVEEDGFIKMFWLDYTEVHGSLCLFGKVQDKKSGKYVSAFLKIDGILRNLYFLPRETRYKNKLDTGEIVAMQDVHDEIAEIMEHHGINFFKAKPSWRRYAFELPGIPKGSDYLKVLYPYTKPPFSHELTGQTFSCVFGTSTALFEQFVLCRNIMGPCWLKIEGANFTAVENSSWCKLEIQVAEPQQVTTIGDADSLEPPPLTLMSIALRTHMNVKDNKQEILAVSCRIYENISLNDANAPEKLSCQMFSVVRPIDPVYPADFEKLVQNHKGTISPQRTESMLLTTFLSKLHQIDPDVLIGHQLEGVDYGILLHRMKERMTTNWHRLGRMKRSAWPQYGGRMAGSFFAERHLVSGRLICDLANDLGKSLTTQCQSWSLTEMCDLILGDKRQDLDNAEALRTWATTGRGLLDYVKHCEADTYFIAAIALKIQIVPLAKQLTNLAGNSWARTMSGTRAERNEYILLHEFYRNKYICPDKVYGKGNTTQKADGAGNEGENEGGGVKKKEKYKGGLVFEPEKGLYDKFILVMDFNSLYPSIIQEFNICFTTVKRDDSGEEKVPDVPETQVLGILPRLISTLVQRRRIVKNLMKDKTATDVQKSQWDIKQQALKLTANSMYGCLGYPRSRFYARPLAMLTTHKGREILRSTKELAEAIDLQVIYGDTDSVMINTGVDNYADAIKIGTDFKRKVNEQYKLLEIDIDNVFQRLLLHAKKKYAAINYVEINGKLATKLELSKDASSHILAEIFSGDDTEVVVERIHEYLRGLAEKVRENAFPLMKYIIYTKLGKSLDEYGTSLQTMPQVQVALRKNARGEPVKSGDVVSYVITGGEGSEAPDKNVSDRAYAPQDIMKTDSGLKPVDAEWYLIKQIFPPIERLCGPIEGTDAMRLAECLGLDTRKYQITNAPDQEDRTIHPLESTLPDEERFKDAVRLQLQCRACGESFQFEGLILSYECCSHEGIVCRNASCNKVLSVPSIVAQVECQIRQLTSKYYEAWLVCDDAACGNRTRQMNVYGKRCLGPNRLADECRGVMGYEYTDKMLYTQLLYFSSLFNVGKATAKPKDGSEKQETITALARTNQPRFEVISNVVQKYLDKCGRQWVSMDSIFAFSVGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6N1</accession>
    <name>D5G6N1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="12">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00002137001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430012">
        <property type="protein sequence ID" value="CAZ80174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836017.1">
        <property type="nucleotide sequence ID" value="XM_002835971.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6N1"/>
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        <property type="interactions" value="474"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G6N1"/>
    <dbReference type="GeneID" id="9187200"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002137001"/>
    <dbReference type="eggNOG" id="KOG0385">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000315_17_3_1"/>
    <dbReference type="InParanoid" id="D5G6N1"/>
    <dbReference type="OMA" id="PNIFTDW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000015">
        <property type="entry name" value="lymphoid-specific helicase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10799">
        <property type="entry name" value="SNF2/RAD54 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="1"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="347"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="233"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="565"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="582"/>
            <end position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ80174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="591" mass="67225" checksum="60E8695C6628BA45" modified="2010-06-15" version="1">MGLGKTLQTISFLAFLREKGTLGPFLVVAPVSTLSNWVEEIARFAPEIPAVLYHGTPPEREEIRKTRMAEMDARFPVICTSYELVMIDRKHLKKHNWKFIIIDEGHRIKNLNCKLISELKKYNSANRLLLTGTPLQNNLSELWSLLNFLLPDIFDDLAKEKKVGIVASLHAILKPFLLRRIKADVETNLPPKREYILYAPLTRNQKDLYGMILDRKAKEWLIEQLLSSRNSSKKRKLLTVGQESPNKRAKSPDPKRKKKCVSYKELSDDEYFNMLEEAPSGEEGLEPTVTEDEKLILQATQEIGTKKLQNRIMQLRLACNSPHLFYWPWGQGEPDERLVTVSGKMMLLERLVPALFARGHKVLIFSQFTKMLDIIEEWAVTLRHWPVCRIDGGIKQEDRREHIRRFNKDEGWNIFLLSTRAGGLGINLTAADTVILFDSDWNPQQDLQAQDRAHRIGQKKPVIIYRFATAATVEQTILEKADGKRRLEKLVIQKGKFRSVSAPSALEEEELAQILKEDFEKVNVVEQGTQLLTDAELETLMDRSPEAYSRAAKGKEEASGAFRVVETGAKEEEEEEAQGADGSAGDGTGGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6P1</accession>
    <name>D5G6P1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA mismatch repair protein PMS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00002148001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA mismatch repair MutL/HexB family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430012">
        <property type="protein sequence ID" value="CAZ80184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836027.1">
        <property type="nucleotide sequence ID" value="XM_002835981.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6P1"/>
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        <property type="interactions" value="710"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G6P1"/>
    <dbReference type="GeneID" id="9187210"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002148001"/>
    <dbReference type="eggNOG" id="KOG1978">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004131_0_0_1"/>
    <dbReference type="InParanoid" id="D5G6P1"/>
    <dbReference type="OMA" id="MRPRRMP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032389">
        <property type="term" value="C:MutLalpha complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000710">
        <property type="term" value="P:meiotic mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16926">
        <property type="entry name" value="HATPase_MutL-MLH-PMS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03484">
        <property type="entry name" value="MutL_Trans_hPMS_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.100:FF:000001">
        <property type="entry name" value="Mismatch repair endonuclease pms1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000014">
        <property type="entry name" value="Mismatch repair endonuclease pms1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000120">
        <property type="entry name" value="Mismatch repair endonuclease PMS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.20">
        <property type="entry name" value="MutL, C-terminal domain, dimerisation subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.100">
        <property type="entry name" value="MutL, C-terminal domain, regulatory subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014762">
        <property type="entry name" value="DNA_mismatch_repair_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013507">
        <property type="entry name" value="DNA_mismatch_S5_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002099">
        <property type="entry name" value="MutL/Mlh/PMS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038973">
        <property type="entry name" value="MutL/Mlh/Pms-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014790">
        <property type="entry name" value="MutL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042120">
        <property type="entry name" value="MutL_C_dimsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042121">
        <property type="entry name" value="MutL_C_regsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037198">
        <property type="entry name" value="MutL_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00585">
        <property type="entry name" value="mutl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MLH, PMS, MUTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073:SF52">
        <property type="entry name" value="MISMATCH REPAIR ENDONUCLEASE PMS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01119">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13589">
        <property type="entry name" value="HATPase_c_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08676">
        <property type="entry name" value="MutL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01340">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00853">
        <property type="entry name" value="MutL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118116">
        <property type="entry name" value="DNA mismatch repair protein MutL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00058">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair protein S5" evidence="6">
        <location>
            <begin position="218"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="MutL C-terminal dimerisation" evidence="5">
        <location>
            <begin position="808"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="380"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="431"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="392"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="471"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="559"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="584"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="631"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="656"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="666"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="678"/>
            <end position="687"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00070941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ80184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1023" mass="112302" checksum="92B6465C926AA9BE" modified="2010-06-15" version="1">MATIRPIEGRSVHQIQSGQVISEGLTSVVKELVENGLDAHATSIEVRFKSNGLDAIEVIDNGDGISPENYESIALKHWTSKLTSYADLDSVTTFGFRGEALSSLCALSDLQIVTATASEAPKGTKLEFEMSGKLKSRSVTAAQKGTAVLVENIFKTLPVRRKELERNIKREYTKVLGILQAYAGVCVGVKFSVFNQPAKGRRTPVFATKGNPTVRENVSNVFGAKALSALVPMNLEFEMKPTRKGFQKAAKPSEKMIKIVGFISRPVVGEGRLAPDRQMFFVNGRPCMLPQVARAINEVYKHFNVTQSPFIFADIQLDTNAYDVNVSPDKRTILLHDQAEMMESLKVSLTDLFDNHEQTVPNSVTQSKTQNLKQMTLDKTPAKPAQAMQQVMEDDSAEEDDASYYAPPKLLNILSSSPSRAAAFDSLSLGQSTAAGSDPKGKGKETHPTPSFRSIKNPDKVSGPSGGPDAADYEDPEGMEEDVMLPSLRLESQGLVHRASTSAVPAASHRSPRRPQQGPATIKIGDREPVTSGGDSPPHSPTKRRKTGNGPASRKGKTTGISKFSSTLSRFAAPGTELPLPTTDNEESEDGDHLMDEDDNSEVVEGEESSSEAVRDGRAEEPATDIMALDGGEKGGDCRDDTEMTAVEPSTKDDSPLFLPNLNPDVDPDDSEEDEETALQSPASSSDAYVEGGYIDEEQNRQQTAQKAERLLKEAEEKPTQPTTESLERALRILEDSHKISTKNAVRFVPTSINKLRDRVHELSKVASTVKYIASPGRGETGLEERDDVAEERLNLAVTKQDFFEMQIKGQFNKGFILATRADDLFIIDQHASDEKYNFEKLQQVTIVQNQRLAVPKKLDLMAVDEIVVIDHIDTFKKNGFVIEVDTGAPVGEKCKLISLPISKETVFGLDDLEELIHLISEDPGNSAVRCSKVRKMFAMRACRKSVMVGKALTEKGMEKLVKHMGELDKPWNCPHGRPTMRHLSDLGRAKYWRGRDQERYGGLKWGSESWAETRLAIEAAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6R5</accession>
    <name>D5G6R5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Autophagy-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00002234001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Autophagosome membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Vacuole membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the ATG8 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430014">
        <property type="protein sequence ID" value="CAZ80208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836051.1">
        <property type="nucleotide sequence ID" value="XM_002836005.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6R5"/>
    <dbReference type="FunCoup" id="D5G6R5">
        <property type="interactions" value="514"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G6R5"/>
    <dbReference type="GeneID" id="9187233"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002234001"/>
    <dbReference type="eggNOG" id="KOG1654">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119276_0_2_1"/>
    <dbReference type="InParanoid" id="D5G6R5"/>
    <dbReference type="OMA" id="AKMKWMF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000421">
        <property type="term" value="C:autophagosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048646">
        <property type="term" value="P:anatomical structure formation involved in morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16128">
        <property type="entry name" value="Ubl_ATG8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000010">
        <property type="entry name" value="Autophagy-related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004241">
        <property type="entry name" value="Atg8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10969">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEINS 1A/1B LIGHT CHAIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02991">
        <property type="entry name" value="ATG8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0072">Autophagy</keyword>
    <keyword evidence="8" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7 9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0926">Vacuole</keyword>
    <feature type="lipid moiety-binding region" description="Phosphatidylserine amidated glycine; alternate" evidence="9">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR604241-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ80208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="173" mass="20210" checksum="692A61AADD4D6342" modified="2010-06-15" version="1">MVGHPTLYSSYPYSPPSSPIVQDKLYQLPKLLASFIYPYPVFKYKSPFNRVFMRSKFKDEHTFEKRKAEAERIRQKYADRIPVICEKVEKSDIATIDKKKYLVPADLTVGQFVYVIRKRIKLSPEKAIFIFVDEVLPPTAALMSSIYEEHKDEDGFLYITYSGENTFGFEELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6W3</accession>
    <name>D5G6W3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00002312001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430017">
        <property type="protein sequence ID" value="CAZ80256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836099.1">
        <property type="nucleotide sequence ID" value="XM_002836053.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6W3"/>
    <dbReference type="STRING" id="656061.D5G6W3"/>
    <dbReference type="GeneID" id="9182480"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002312001"/>
    <dbReference type="eggNOG" id="KOG4231">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_144_2_1"/>
    <dbReference type="InParanoid" id="D5G6W3"/>
    <dbReference type="OMA" id="VGFNNPC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047499">
        <property type="term" value="F:calcium-independent phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019369">
        <property type="term" value="P:arachidonate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046486">
        <property type="term" value="P:glycerolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07216">
        <property type="entry name" value="Pat17_PNPLA8_PNPLA9_like3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24185">
        <property type="entry name" value="CALCIUM-INDEPENDENT PHOSPHOLIPASE A2-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24185:SF1">
        <property type="entry name" value="CALCIUM-INDEPENDENT PHOSPHOLIPASE A2-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 4" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3 4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="6">
        <location>
            <begin position="12"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="187"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXGXXG" evidence="4">
        <location>
            <begin position="16"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="4">
        <location>
            <begin position="64"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="4">
        <location>
            <begin position="272"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="66"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ80256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="631" mass="69167" checksum="E7AC6C864E95948C" modified="2010-06-15" version="1">MAESNLPPLRILSLDGGGVRGLSSLMILQDIMSTISQQEKSSHIRPKDDDTPLKPCEYFDIIGGTSTGGIIAILLSRLRLDVPACIDIYTQLAEEIFSKDRSIKIFGTPLRFAGARFSGAVLEKAIKRVLVENSFDPEEKMFDETLTLREKEIRRRVDTGQLTKLEVTHSGGVRDVIEKKTWRTSTNSNFSLKPAQDSKPDPNTHSPQTPKPGCHGIVVAVYKHAVGVPKLFHTNDPDDRKTKIWQALRATSAAPTFFDEITFGTPKITYIDGGLGYNSPCAEIDFEAKTRWPGREIGVIVSIGTGLQTIPAIRRGLRWLPFGLHRELSLAGALVAMATSTARVDNEVQRMYRDAQTEYYRWDVDSGLGEISLEQWMKEDEMGSVTRRYLDDPDQVIRRQKLARTIVGISTKRRVVECSAGRFTVTTIPAGEKAASWSLESLSLPGVPGNSRAGPIPVLEDLDHDGTRAESQILTCNRALTSSVDLRTLISGVPTGRYKARFILRSSIPVSSSSPLRPPGDLILSAGRPYDAKTFTNRFVDRYISLDVVPALLYPDAARIRVTAQEWAVRSEQSGNSGWFEVSGDAEVEVCAAGCVGVVVSCLMRNGGDGEGDDGQICGGWGFGGVRLVPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6Z2</accession>
    <name>D5G6Z2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00002392001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the RecA family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430019">
        <property type="protein sequence ID" value="CAZ80285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842550.1">
        <property type="nucleotide sequence ID" value="XM_002842504.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6Z2"/>
    <dbReference type="FunCoup" id="D5G6Z2">
        <property type="interactions" value="749"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G6Z2"/>
    <dbReference type="GeneID" id="9184099"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002392001"/>
    <dbReference type="eggNOG" id="KOG1433">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041732_0_2_1"/>
    <dbReference type="InParanoid" id="D5G6Z2"/>
    <dbReference type="OMA" id="RAYNSNH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000794">
        <property type="term" value="C:condensed nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000262">
        <property type="term" value="C:mitochondrial chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905334">
        <property type="term" value="F:Swi5-Sfr1 complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070192">
        <property type="term" value="P:chromosome organization involved in meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000730">
        <property type="term" value="P:DNA recombinase assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990918">
        <property type="term" value="P:double-strand break repair involved in meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000086">
        <property type="term" value="P:G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030491">
        <property type="term" value="P:heteroduplex formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007533">
        <property type="term" value="P:mating type switching"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000709">
        <property type="term" value="P:meiotic joint molecule formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000708">
        <property type="term" value="P:meiotic strand invasion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043504">
        <property type="term" value="P:mitochondrial DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990426">
        <property type="term" value="P:mitotic recombination-dependent replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036298">
        <property type="term" value="P:recombinational interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120290">
        <property type="term" value="P:stalled replication fork localization to nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000722">
        <property type="term" value="P:telomere maintenance via recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010995">
        <property type="entry name" value="DNA_repair_Rad51/TF_NusA_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013632">
        <property type="entry name" value="Rad51_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020587">
        <property type="entry name" value="RecA_monomer-monomer_interface"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22942:SF39">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD51 HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22942">
        <property type="entry name" value="RECA/RAD51/RADA DNA STRAND-PAIRING FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14520">
        <property type="entry name" value="HHH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08423">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47794">
        <property type="entry name" value="Rad51 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50163">
        <property type="entry name" value="RECA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecA family profile 1" evidence="5">
        <location>
            <begin position="1"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="RecA family profile 2" evidence="6">
        <location>
            <begin position="197"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ80285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="265" mass="28389" checksum="A339B9371A7C2177" modified="2010-06-15" version="1">MSAEEVAQEGGSQFDDQSTQIGGPGAPTPLSALEGNGISARDIKAVVEAGYNTVEAVAYTPKRALLVIKGISEAKADKLIAEASRLQAVSKQAPSQSQLCHTLAVLDNVAYARAYNSDHQLQLLNQAASMMTETRFSLLIVDSATSLYRTDFAGRGELSARQTHLAKFMRTLQRLADEFGIAVVITNQVVAQVDGGAAMFNPDPKKPIGGNIIAHASTTRLFFKKGKGETRICKIYDSPCLPESECMFAINEDGIGDPNPKDLEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6Z6</accession>
    <name>D5G6Z6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00002396001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">RNA-binding nucleolar protein required for pre-rRNA processing. Involved in production of 18S rRNA and assembly of small ribosomal subunit.</text>
    </comment>
    <dbReference type="EMBL" id="FN430019">
        <property type="protein sequence ID" value="CAZ80289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842554.1">
        <property type="nucleotide sequence ID" value="XM_002842508.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6Z6"/>
    <dbReference type="FunCoup" id="D5G6Z6">
        <property type="interactions" value="898"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G6Z6"/>
    <dbReference type="GeneID" id="9185915"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002396001"/>
    <dbReference type="eggNOG" id="KOG2050">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013994_1_0_1"/>
    <dbReference type="InParanoid" id="D5G6Z6"/>
    <dbReference type="OMA" id="YGPEFSI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012959">
        <property type="entry name" value="CPL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033133">
        <property type="entry name" value="PUM-HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040059">
        <property type="entry name" value="PUM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13389">
        <property type="entry name" value="PUMILIO HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13389:SF0">
        <property type="entry name" value="PUMILIO HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08144">
        <property type="entry name" value="CPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00806">
        <property type="entry name" value="PUF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50303">
        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="PUM-HD" evidence="6">
        <location>
            <begin position="127"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="4">
        <location>
            <begin position="191"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="4">
        <location>
            <begin position="341"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="44"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="90"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="100"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ80289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="659" mass="73008" checksum="B930B5053CFDBFB1" modified="2010-06-15" version="1">MGASKRKQEKTQSATTHKKPRVSEPKIESKTSKKSVLPPRVVESDSDEPDEGGEEEIGNSDEDSEEEFGGIEDADEDEEMNTGDKEKSKNPGNGASGSSKSKESHTEQKRLAAERKLAKPYGEIITRSKRIWEQLRRTKLAPEERKKLIAELGQLIKGRVNGLVFRHDASRIVQSALKYGDKTTKTNITTELKGSYVSLAQSSYGKYLVVKILHYGTPENRKMVVQEFYGHVRKLTKHREAALVIEDVFREYATPQQKSALLQEFYGVEFAIFKDLKHKPSLKELLEKSPEKRTVIMKSLFDQIHGVIKKGATIFTILHKAMLEYTLNTRAGTTETTEFMELVKEHVADIAFTKDGSQVVIRCMALGTAKDRKVMIKALKPEAISLARSEYGYLVLLSILETVDDTVLVVKSLLPEFQKRLQELACDKYGRTPLLYPFSERHPRVVPRPALAVLEEIEKLRESTSKKDPATRQSELRTHFSQPFLQCIAEHAEELIRDSFGCQFITEVLLGSTGDKSPALQALASTAAGNPKTADDGDDPHVVNTAAVGRMYKTLVTGGHYSSKENKVISITPPLNFHSLLWEQIRPHVNAWAAGAGSFVVVALLEAEGFPARQEVVKALNRKKLRKAAEEAGNKGANVMLGILEEGGGGGGAKGSEAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6Z8</accession>
    <name>D5G6Z8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00002399001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430019">
        <property type="protein sequence ID" value="CAZ80291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842556.1">
        <property type="nucleotide sequence ID" value="XM_002842510.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6Z8"/>
    <dbReference type="STRING" id="656061.D5G6Z8"/>
    <dbReference type="GeneID" id="9185917"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002399001"/>
    <dbReference type="eggNOG" id="KOG1356">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_301915_0_0_1"/>
    <dbReference type="InParanoid" id="D5G6Z8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032454">
        <property type="term" value="F:histone H3K9 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045109">
        <property type="entry name" value="LSDs-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12549:SF38">
        <property type="entry name" value="JMJC DOMAIN-CONTAINING HISTONE DEMETHYLASE 2, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12549">
        <property type="entry name" value="JMJC DOMAIN-CONTAINING HISTONE DEMETHYLATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="JmjC" evidence="5">
        <location>
            <begin position="579"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="860"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="15"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="28"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="68"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="111"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="153"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="904"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="936"/>
            <end position="955"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ80291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="989" mass="108979" checksum="8753A8D92479CA9C" modified="2010-06-15" version="1">MARRKKAKPRVATVAPTSSSRGTGNASSRERVALRTDIERKKTSNLSDSGGDNENHEVISGGGFADPGSSNPSADYALQGTSSDQVATGRSRQTRSTSLPLVNYKVDSDSSESSQMSIDSDYVASGTSSSRINKAATGKKRVPVDCSGDEEFESVKRPKEKPTPKRMRNSITKGNGKPSTPVPGKRKGRAFPLKDDLPCGYVALGPSPEFKYVQKGPCQRNKKLPEHCEACISKSVKTGGCRFSGIRKFKYDYYGSAESDPTDAEFCVADDQMSLEQLSQAEDQQREMVFNSADAETILPLIGVNLLAKLSSELYHEEFHSGILKKTNLDGGPRPLVRLKKITLRTICDICSTTTLFGTYLSGCCGREICLDCWTEWTPVVKDGDPTMPDKCTNQRLHNKDSFYFATRAYPGEIKFFISSIRAYRDRVGLKPNDDNPVPDIPINTPIHPGEKGLVYLGTPTDFEEAVSKVQFQTLWKKGGIPLVIKGLKKKFTLPWDPEFFIEMYGGKPCAITDCGTGQVGVSTVGDFFRDFSKTDVEDTGTLRSLKLKDWPPESDFKDEFPNLFADFERALPFPEYTNRDASLNLVSRLPADWTKPDLGPKMYNAYPAPDFIPVKNGPPNPVKGTTNLHFDMTDAVNILVHQSGGPTPAANKRWKEVYGYPKCGAIWDIFPPESSAAIRRFLKKRDASVDDPLNRPLFYLTEEDLIELGKPEYNVRSYRIYQSTGDAVFVPAGCPHQVRNKQSCIKVAVDFFSAENAAVCTDLLADFRALAKATTKKGGMRLVGKRDDVLQPYNCLLFNWISLTGVSFERAVKTHKVDLANGKLIDEPSDSGSELTDLDEIEHEDFMLDYDLKSSEQSVSIQEDSGVGAESHRGNRKRAGLSNLDGDAAPMTNSKRVKKEHVPSTTGQKKANQRGSTAELDSNCDDDDVDAPQPRAKRVKRVSVRAPHKRRKVKGQVVTEVSTLEALSPSGSELGVRTVGPGLHLTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>D5G723</accession>
    <name>D5G723_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00004567001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430020">
        <property type="protein sequence ID" value="CAZ80316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836125.1">
        <property type="nucleotide sequence ID" value="XM_002836079.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G723"/>
    <dbReference type="GeneID" id="9182507"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004567001"/>
    <dbReference type="HOGENOM" id="CLU_1074389_0_0_1"/>
    <dbReference type="InParanoid" id="D5G723"/>
    <dbReference type="OMA" id="FMCASFA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072227" evidence="2">
        <location>
            <begin position="18"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="121"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ80316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="259" mass="26070" checksum="4D8050655F4FADBC" modified="2010-06-15" version="1">MRFSILPTLLIVTSVSAVSLSDISPLPSNSVTPGCESAYGEPMPACPSDLRASKQCSAGCSAALDETAKKVIVACRQAFIGPDTLLRRVLDGGIQKTLCGAAVSTPVYVASSTGSDSVPGAKTTTTTASPTSTSTSASTLTKSTVPTRAPSFSGSMTLSIERPTGSSSLAGLLTLDNSPAPTGSFETADSSFTRIFYNPNSTSTAESASIAAASTTSNGMSGNGSPFTSGEINSAPPSSINAQKGAWVVFALLAAFLCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5G778</accession>
    <name>D5G778_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00002503001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430022">
        <property type="protein sequence ID" value="CAZ80371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G778"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002503001"/>
    <dbReference type="HOGENOM" id="CLU_3421427_0_0_1"/>
    <dbReference type="InParanoid" id="D5G778"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ80371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="24" mass="2600" checksum="8ABCAB1A3F363253" modified="2010-06-15" version="1">MIVFRSILFSIRCTLTGICGVVGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7T2</accession>
    <name>D5G7T2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="1">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00004713001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="1"/>
    <dbReference type="EMBL" id="FN430031">
        <property type="protein sequence ID" value="CAZ80575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836384.1">
        <property type="nucleotide sequence ID" value="XM_002836338.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7T2"/>
    <dbReference type="FunCoup" id="D5G7T2">
        <property type="interactions" value="905"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G7T2"/>
    <dbReference type="GeneID" id="9181394"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004713001"/>
    <dbReference type="eggNOG" id="KOG0417">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030988_13_2_1"/>
    <dbReference type="InParanoid" id="D5G7T2"/>
    <dbReference type="OMA" id="AEPHEDN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd23813">
        <property type="entry name" value="UBCc_UBE2N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000020">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="8">
        <location>
            <begin position="2"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ80575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="149" mass="16988" checksum="7D2AE52A7B9083BF" modified="2010-06-15" version="1">MALPKRIIKETERLVAEPVPGINAVPHEDNLRYFDVTIHGPTQSPYEGGIFRLELFLPEDYPMTPPKIRFLTKIYHPNIDKLGRICLDVLKGNWSPALQIRTILLSIQALLGAPNPDDPLANDVAQRWKEDERAAIETAREWTMTHARV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7Y4</accession>
    <name>D5G7Y4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00002690001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430034">
        <property type="protein sequence ID" value="CAZ80627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836436.1">
        <property type="nucleotide sequence ID" value="XM_002836390.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7Y4"/>
    <dbReference type="STRING" id="656061.D5G7Y4"/>
    <dbReference type="GeneID" id="9184103"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002690001"/>
    <dbReference type="eggNOG" id="KOG2894">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037985_1_0_1"/>
    <dbReference type="InParanoid" id="D5G7Y4"/>
    <dbReference type="OMA" id="DFIWVFL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048337">
        <property type="entry name" value="FAM50A/XAP5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007005">
        <property type="entry name" value="XAP5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12722:SF0">
        <property type="entry name" value="PROTEIN FAM50A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12722">
        <property type="entry name" value="XAP-5 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04921">
        <property type="entry name" value="XAP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAM50A/XAP5 C-terminal" evidence="3">
        <location>
            <begin position="183"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="45"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="270"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="159"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="85"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="101"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="124"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ80627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="338" mass="37715" checksum="50A972960CDCA073" modified="2010-06-15" version="1">MSGPAASTPSRFVANTETLEDVLKTQTVGLVHLSEFKKRRVELAEQRDREAAEKLQPIRSSGGGGGSSSSREGSEAPTGSSGGKRTNKRRKVVKGKLSFAGEDDGDDGTDDGGDSSSADPTRSNSEDNNKSGEEDEVKKRKVNPKLGLPAPKVLTKNTLLREAQERETLRREFLALQEKIKNEEISIPFVFYDGTNVAPPTGEGVTVKKGEAVWLFLERARRMSGRREWLRVSVDDLLLVRGEVIIPHHFEFYYFIVNRTMGPNGLLFDFPSSPSPTPQTPTTNPNRDDPTMTKVVDRRWYERNKHIFPASVWTEFDPNTDYSNMVRRDMGGNAFFFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>D5G807</accession>
    <name>D5G807_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00002774001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430037">
        <property type="protein sequence ID" value="CAZ80650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836459.1">
        <property type="nucleotide sequence ID" value="XM_002836413.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G807"/>
    <dbReference type="STRING" id="656061.D5G807"/>
    <dbReference type="GeneID" id="9184123"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002774001"/>
    <dbReference type="eggNOG" id="ENOG502SA0I">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_326832_0_0_1"/>
    <dbReference type="InParanoid" id="D5G807"/>
    <dbReference type="OMA" id="RWRASVM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="181"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="299"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="475"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="508"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="611"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="667"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="148"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="265"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="325"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="371"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="482"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="508"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="622"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="743"/>
            <end position="754"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="881" mass="96507" checksum="26923D7069208413" modified="2010-06-15" version="1">MSYSVISQTTSYRRRSSIASSTSTNASYPPPELQLLNSEAPYLPPSPESRPVQTEYSPTDRVMSMLKSSVLSGTSYEMVGDSSDEEGDLRSGRGAQGSDATGDNRRTNGRRDSGDMDREADEAFSGMGGEGIVGSRVSVSDPPHSKSDSASQGATSSVSYGDHRTELEIAERDLRLELHLDGRADISSPQRGDVSDPQTARGVHRPYQHHIPSGSRSSHQLPPLPVSAGILDPEDQAFGFTASPEDVHPAFRPSQSYPELPLPPKADHAREQREYRPATSATNIGDLFEDFDGVHFDLDHPNNRFSVAPPPPPHRHRQRQSQHSIHPTSPHSDSQGSSNLVFYPAPVPAMLNLPPTLSKPKSTANNRHSRHIPDNADPTDNRRSVAYSVAGAPGHVPDKDLHRRSVATLDQAANRRSIANLPPALRASQFFDIPTIPQIEVKEDSAVATLDSILDASAKAPPAAFTDHPIAGGAVPYGPKGNRSSMHLPSGPNNNYRNSVAITVKLHPGESPRSSMEDEIHEDARSHLNPETSEYYDPGNQHRHSGISEDFAPQPDPDLGGGLPTTLLAELESRKAQQRLRNRTAASAFPGGIRSTLLQLDAVAQVQKEARSRKKTHLAWEDPGDVDEDDDQDGDVPLGLLYTVAPGAQTRRDDEVPLGLLMKKQMEDAEPLSKRRERLQGQNGHVVQPQPRRFLDLPGLGGGSQTSAADAEEEVEGETLKDRMKRLKERKEFGTGTLGMEFTSPSEGNGSTAKQEPAPPATEETLAQRRRRLQQQEATKHESSLLQQEVRNRRNMMDLLYRANNRPQSYYSQAGNNAGYIQPQPQMGGLMGNQYNMQMGRGMPMPMQMQMPMPMNMGGTGEAILDPKQREVVERWRASVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5G858</accession>
    <name>D5G858_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="11">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00002842001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transient receptor potential (TRP) ion channel family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430040">
        <property type="protein sequence ID" value="CAZ80701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836510.1">
        <property type="nucleotide sequence ID" value="XM_002836464.1"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G858"/>
    <dbReference type="GeneID" id="9188303"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002842001"/>
    <dbReference type="eggNOG" id="ENOG502R2RV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004278_0_0_1"/>
    <dbReference type="InParanoid" id="D5G858"/>
    <dbReference type="OMA" id="DIWAGMM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010308">
        <property type="entry name" value="TRP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040241">
        <property type="entry name" value="TRP_Flc/Pkd2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032800">
        <property type="entry name" value="TRP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31145">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_7G01610)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31145:SF6">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_7G01610)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06011">
        <property type="entry name" value="TRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01320">
        <property type="entry name" value="TRP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072580" evidence="9">
        <location>
            <begin position="18"/>
            <end position="1282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="268"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="400"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="457"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="482"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="560"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="592"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="623"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="domain" description="ML-like" evidence="10">
        <location>
            <begin position="16"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="67"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="698"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="794"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="993"/>
            <end position="1241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="804"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="838"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="1006"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1023"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1035"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1095"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1115"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1128"/>
            <end position="1146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1178"/>
            <end position="1191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1198"/>
            <end position="1221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ80701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1282" mass="138062" checksum="6F547C8CFCCC4841" modified="2010-06-15" version="1">MLLLLVVLALFAGSARSVLISTFDNCLSDDVVRSPTDIQFHPLVVAAAFGTKDKDLLQITVWGNVTGDNSSNPRGRHRRDLRGSDGHFQPRAGDWPWLEARQEVANSQNLNGSAVTGQPGTLNWGLANFQNPTFDSDVQYRNSGLIVHSDQSWPKATTLQPKIQIASFDTSAGAIFLCTVTGFPNARAPCPLKTTKNMSKSDAHNLTVLMKELPSFTWEYNFTSGYSFASIDTTFTIISGNSDKTLFGCIHVETTPMLGKSNDAALTWVPAGVLALVALGTILAAMLNPWVGTTDIFRWSSNYGMDEDMIRLVTPGFADCLQWLQFFVLTGSLTLSYPGFYQPVVSRVAWSALLFNTSFFSHDPISQRRWAGDGLYALDATAYGFERVAQAVGLQTIDDIWVCVMAFFGVVAIGTVVMPQLWFAGKWIVRKAMGHEEGDLTNKNLPFTFGMLNRITYTYFLTPILAASIFVLTTPGRGSNTIVALAAALVLVIIAVAVLLTIRIWSHKPRSTLFDNLPTLLTFGTFYNTYREKNLKFFIVQLFVNISRAIAFGALQPSGIAQITILAICEIVLILTVCGIKPYAPATSMNAWQAIFAVIRLLTILLMMAFVPSMDAQDAAKSWIGYVILVIHALVLIFGFFFMAVQTLLELFIRGCGGTDEGEAARGGLAKVFGMRQLSRRKHKQHRGSVDPSIINVSTHDEHKMSSTPDNRPAGVGPRSHSGSSAVLLGGPGVPTGAPGMMDVGNQTNHHHSGSGSTGFTTTTPGTASNFSYMGVAAGPPLAVDTGATTVASPYYRPPRATGGTYGLRNSGGGSGSDNYSPGTRSKGSWGSGMWGTTDASGGMQRSSQGSLPNGPDDLWNHGPGPSSGTGTPQGGAADISRGELQPLAHRHTDNSLTAGSVRNTAQTDYTIREMDYYYGVRGPALNDQPGRRLGTGPADPTGPVAVTKGWLWKRLGWGFGGKKETGFIVDRSSRAPEALLDAERAAKGIGMAVTSQTPPVPDQSDSSDSDDDTGDEEEAGKSSKRHRRRRKTPSPMSHLTGRSNGRRKSLSLISQNTDSDSEGEDGGEAAGPSSRMLEKKPAAAYDGSNNIELQRQDSIKPEVPRKSSKRKSVTYLTAFNSNPPVSTSPQPHQPPLQQQPLQPTGSPGGPVQARLPHHPYDPPPLRPSLHPRHPQYDTRNLTPTHYNGNPSGRLPFTTTSSTQEGQESTHTRSISTSSSLLPPPPEITADDNALSRPPSVGTVHRYRMEDSLRNSDVVEGGRSGSLVYEGGGGWRGEGGWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8H1</accession>
    <name>D5G8H1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">triacylglycerol lipase</fullName>
            <ecNumber evidence="6">3.1.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Autophagy-related protein 15</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">GSTUM_00002862001</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12044"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17855"/>
            <dbReference type="ChEBI" id="CHEBI:18035"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="EC" id="3.1.1.3"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Binds to both phosphatidylinositol (PI) and phosphatidylinositol 3,5-bisphosphate (PIP2).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endosome</location>
            <location evidence="3">Multivesicular body membrane</location>
            <topology evidence="3">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Prevacuolar compartment membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.3" evidence="6"/>
    <dbReference type="EMBL" id="FN430043">
        <property type="protein sequence ID" value="CAZ80814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836623.1">
        <property type="nucleotide sequence ID" value="XM_002836577.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8H1"/>
    <dbReference type="STRING" id="656061.D5G8H1"/>
    <dbReference type="GeneID" id="9186033"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002862001"/>
    <dbReference type="eggNOG" id="KOG4540">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1403362_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8H1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032585">
        <property type="term" value="C:multivesicular body membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005775">
        <property type="term" value="C:vacuolar lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004620">
        <property type="term" value="F:phospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034496">
        <property type="term" value="P:multivesicular body membrane disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046461">
        <property type="term" value="P:neutral lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006660">
        <property type="term" value="P:phosphatidylserine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050805">
        <property type="entry name" value="ATG15_Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47175">
        <property type="entry name" value="LIPASE ATG15-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47175:SF2">
        <property type="entry name" value="LIPASE ATG15-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0072">Autophagy</keyword>
    <keyword evidence="8" id="KW-0967">Endosome</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00029828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAZ80814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="194" mass="22141" checksum="1DC1D072E89CB063" modified="2010-06-15" version="1">MCFTMQPIGEKCSLRNYPYQFRRLDIHPDTLVATENDPQLRPRPRHRLSSRTRPISRLVDRSKETIEDMLYAHRAHHPRTFSDDDWTTEIIPMPNITDKTTVLNFAHIAADAYIEAEGTEDWLDIGQPYNATSNFGWEGDGLRGHIFADEANTTVIIGLKGTSPAVFDGAETTTNDKINDNLFFSCCCARVSYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8I5</accession>
    <name>D5G8I5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00002889001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430046">
        <property type="protein sequence ID" value="CAZ80832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836641.1">
        <property type="nucleotide sequence ID" value="XM_002836595.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8I5"/>
    <dbReference type="STRING" id="656061.D5G8I5"/>
    <dbReference type="GeneID" id="9186052"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002889001"/>
    <dbReference type="eggNOG" id="KOG1414">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034316_1_0_1"/>
    <dbReference type="InParanoid" id="D5G8I5"/>
    <dbReference type="OMA" id="CESTWID"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14687">
        <property type="entry name" value="bZIP_ATF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000053">
        <property type="entry name" value="BZIP transcription factor AtfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051027">
        <property type="entry name" value="bZIP_transcription_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002112">
        <property type="entry name" value="Leuzip_Jun"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021755">
        <property type="entry name" value="TF_Aft1_HRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021756">
        <property type="entry name" value="TF_Aft1_HRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020956">
        <property type="entry name" value="TF_Aft1_OSM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19304">
        <property type="entry name" value="CYCLIC-AMP RESPONSE ELEMENT BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11786">
        <property type="entry name" value="Aft1_HRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11787">
        <property type="entry name" value="Aft1_HRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11785">
        <property type="entry name" value="Aft1_OSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00043">
        <property type="entry name" value="LEUZIPPRJUN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="8">
        <location>
            <begin position="454"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="361"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="479"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="34"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="50"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="145"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="421"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="438"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ80832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="574" mass="59338" checksum="34A13F39CF299383" modified="2010-06-15" version="1">MSAAVVSRQQPGSAGHSTGGTSNSNPRMGTHSPVDSKKSNSKSEDKQRPSSDQSNNNGRDTAKNSSKVASHTLSPLSNSSLPVASSIHPPQVAVDQPPDATKPSPQNNHGPGPSRSLPNKKLSGDTPGDYFTTTHTRLDQEPNPFEQSFASTSDPLSTPKTILPPVTAITSPASLLPGGSGGSAFNWGLNSLRSGPLSPAMLQGPASGSNVGSSSSLAFDTHIRTGLTPNESGIRSGLTPGGSGSLFAPSSAGSVLFQQFGGGGATPGTMEFQRTALLAARKSSHPAPSLMSPQQQHQLDNYHAADPAHNAANGLFLLANAQHQQQEAAGASGAGMNTGSALSYPGGPASSGATAIDGSRISEISDGFGGSSDEEMQNTGSRRNTRPKNPKGKQSAASSGRRKADDSPGGTGRSQAKKVKGPQGQSMSVSGGSDDGQDDGKDENGKDTKKMTDEEKRKNFLERNRVAALKCRQRKKQWLASLQAKVEIFSSENDALTAQVTSLREEIVSLKTLLLAHKDCPVARSNGGNMATLDMNTGMNNDYGHVPMMTSHYGGMTGMGGGVMAPPGAGRRYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8N0</accession>
    <name>D5G8N0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00003026001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430048">
        <property type="protein sequence ID" value="CAZ80873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836682.1">
        <property type="nucleotide sequence ID" value="XM_002836636.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8N0"/>
    <dbReference type="GeneID" id="9186241"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003026001"/>
    <dbReference type="eggNOG" id="ENOG502RJSF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_667632_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8N0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072592" evidence="3">
        <location>
            <begin position="25"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="223"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="196"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="253"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="363"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="256"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ80873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="412" mass="42143" checksum="E0C8CF9CFE916113" modified="2010-06-15" version="1">MNLQQRELFFLFAILGVACIVVDGGQISERAIILEKRQMETTPADQITEVTTLVDTVLSPGTTKSAPVTITRVSTSLGTASRTSSITLTSSSVSPSPTSSALYCNAGYTLCGAEFGGGCCKTGYTCALTGCVPPAGTTTPPPSRTCTISSQTLCASSMGFGCCVVGQECGFSSCTPAGGSAVTKVPDVDANNLKLSATATTSAPSPTPTESKSKGDDAPLSKIAIGGIVGGSVVAIIALALAWMFTRNHIKKRSNTDASGGVPTTNVFDPTATLSGQHGSHSSRYRGTQPPTAVAAATKITGGPKMSEYHPHHPAKGPTAVPPAHSPPLQHSPPAGDQRFDFGIPGVGGHGYQPVPLTDHMPAELGASPVEGWGMGELQGEGNPVYEVEGDGGGRSPVGKRKSWGWRNSGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8U4</accession>
    <name>D5G8U4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00004844001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430053">
        <property type="protein sequence ID" value="CAZ80937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836746.1">
        <property type="nucleotide sequence ID" value="XM_002836700.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8U4"/>
    <dbReference type="STRING" id="656061.D5G8U4"/>
    <dbReference type="GeneID" id="9186446"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004844001"/>
    <dbReference type="eggNOG" id="KOG4197">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_362551_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8U4"/>
    <dbReference type="OMA" id="HKESERW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140053">
        <property type="term" value="P:mitochondrial gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47938:SF35">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN 4, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47938">
        <property type="entry name" value="RESPIRATORY COMPLEX I CHAPERONE (CIA84), PUTATIVE (AFU_ORTHOLOGUE AFUA_2G06020)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="PPR" evidence="1">
        <location>
            <begin position="386"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="1">
        <location>
            <begin position="421"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="50"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ80937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="771" mass="88082" checksum="6D7E993BEB98EF26" modified="2010-06-15" version="1">MMIPHTHAVAPSKNALHYLRQILFHWPCESFHRGPTANCIRLDVRRENSNCSQQAKVRSPEVPKKQRNPICLEAPPSSPNVSLTKPAPKSVSRRHLLISQRASAQGAATTTAATTNTTTATSIAIPIQDKIKSASSRIWVLKCCRSILEGKPSEAREGFLAGLRRGEDGEQVKIVGKAVMKALRKRGRHSEIPDIFHRMHIAGQKHVDTYALNICLEAYIEMGMYSKVISTYRHHRPRVRPDIQTMRHRLRNLIAMRKLHQAEKLLRRLLREEGPPWMADSTMFALLLGGVKIISGSFHRMERIFKWMAESRVKLHVGVFNILIEAAIDIGRLDIAKGYAQVKLEFGIKHNAETYAIFLAAYARKQNWQGTANVLKEMGVRKVQLTAPMFNMLLKTYSTMIGLQDLEKFFDEMEKRGILPDSYSYNIMIHSCVRQWDEPSIEHWVRRLVSSGIEPDAATLNTLFQTIRYTGDAVPSLLRRIYAIIRESNDSLINQRTKDMLVHSTYRGLQRVRIRPSQEHVDQSREEVAMDRALQQGQFITAIDIFQKHLSRGIKPSIPIVTSAVRATLSLPRPNLPEAYRLLRICEDRGIKISKTPTLRSFEVLLPPRNTDPAEAYIAVSSQVREAYTFLESAELPITHDISVHTAWRLIQEKNPRAAVLLLHKISKTRWGTDFPWEKKSLTVLMKAYIDIGHISGLGWIVDQLIDSGEVPDGVVFKYLKRAVDAATTDQDKTFAQWLGARCFKHKSSMREEAKRRMHFLWYLAKNWKKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8Z0</accession>
    <name>D5G8Z0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00004902001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430053">
        <property type="protein sequence ID" value="CAZ80983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836792.1">
        <property type="nucleotide sequence ID" value="XM_002836746.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8Z0"/>
    <dbReference type="STRING" id="656061.D5G8Z0"/>
    <dbReference type="GeneID" id="9181266"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004902001"/>
    <dbReference type="eggNOG" id="ENOG502SJ6K">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019101_2_2_1"/>
    <dbReference type="InParanoid" id="D5G8Z0"/>
    <dbReference type="OMA" id="VVIMVTY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF110">
        <property type="entry name" value="UBID FAMILY DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="88"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="131"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="174"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="211"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="245"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="8">
        <location>
            <begin position="29"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="278"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="278"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ80983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="374" mass="42006" checksum="DAA2F05F35F8EB5D" modified="2010-06-15" version="1">MALTSPTPEQLTVESWSMYSVGVLLITGRIYSRCLILNGFRNLQTEDFLMIFNAVMYTILMAAINVSGRTATNLLEPGTLETLTPDDIQIRIYGSKIVILLEQAMLILIWGIKGCMILMYNRLTTRLQQQMLVRAVGVYVAAGFVAIELAWFLSCRPFNGYWALPVPMEQCATYQHYSIVQASFNISSDLMMLGIAIPLFLRARVPNKKKLILVGIFGCGIFVILAAILNKYYNFSNPFTTIYMLWYIREASTAVYVSNLPMLWPLFRRAFNIGTFQERSTPKNNSQPLSDMSRKKGTLTSLAGSEDRITREPALEIDQRISFTVEEYRGEGKGGDVDLEMACKVSQESIRPHSGYGRGVCVHNYKAEVSAGTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>D5G926</accession>
    <name>D5G926_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00004956001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430053">
        <property type="protein sequence ID" value="CAZ81019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836828.1">
        <property type="nucleotide sequence ID" value="XM_002836782.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G926"/>
    <dbReference type="STRING" id="656061.D5G926"/>
    <dbReference type="GeneID" id="9181302"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004956001"/>
    <dbReference type="eggNOG" id="ENOG502QPT2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1305677_0_0_1"/>
    <dbReference type="InParanoid" id="D5G926"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46211:SF14">
        <property type="entry name" value="GLYCEROPHOSPHODIESTER PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46211">
        <property type="entry name" value="GLYCEROPHOSPHORYL DIESTER PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GP-PDE" evidence="1">
        <location>
            <begin position="25"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="211" mass="23630" checksum="1E3E2A72942690C4" modified="2010-06-15" version="1">MHYRHWTVEVSNFRIMIKPSIAVHPRAKISTLEEVLDLIDCYGDNKVEINLETKIDPEHANETLPIEVYINNLVPILQRRGYADRTYIQSFDWRTLIGIKKKFPETRIVALLDDTTVIASGNLGFPWLGGIDLQKDFAGDWVKAAHSINATALSPVHGTPSSASVNTPGYTPFTTAAVVSRAHELGMAVIPWTVDDEVTIEKLFRRAIHAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>D5G946</accession>
    <name>D5G946_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Succinate dehydrogenase assembly factor 3</fullName>
            <shortName evidence="8">SDH assembly factor 3</shortName>
            <shortName evidence="8">SDHAF3</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00003123001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Plays an essential role in the assembly of succinate dehydrogenase (SDH), an enzyme complex (also referred to as respiratory complex II) that is a component of both the tricarboxylic acid (TCA) cycle and the mitochondrial electron transport chain, and which couples the oxidation of succinate to fumarate with the reduction of ubiquinone (coenzyme Q) to ubiquinol. Promotes maturation of the iron-sulfur protein subunit of the SDH catalytic dimer, protecting it from the deleterious effects of oxidants. May act together with SDHAF1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 8">Interacts with the iron-sulfur protein subunit within the SDH catalytic dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the complex I LYR family. SDHAF3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430054">
        <property type="protein sequence ID" value="CAZ81039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836848.1">
        <property type="nucleotide sequence ID" value="XM_002836802.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G946"/>
    <dbReference type="STRING" id="656061.D5G946"/>
    <dbReference type="GeneID" id="9184326"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003123001"/>
    <dbReference type="eggNOG" id="KOG4100">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1518404_0_0_1"/>
    <dbReference type="InParanoid" id="D5G946"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034553">
        <property type="term" value="P:mitochondrial respiratory chain complex II assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006105">
        <property type="term" value="P:succinate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20270">
        <property type="entry name" value="Complex1_LYR_SDHAF3_LYRM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008381">
        <property type="entry name" value="SDHAF3/Sdh7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13137">
        <property type="entry name" value="DC11 ACN9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13137:SF6">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE ASSEMBLY FACTOR 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13233">
        <property type="entry name" value="Complex1_LYR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU368039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ81039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="194" mass="22607" checksum="2C80467A683C3E5B" modified="2010-06-15" version="1">MAPSPTTVKLMFSIFPMQYDIHQDRVIAAFAIVCDRRKPPESYTLTHSHTSFYSPPFPSWTKCAKRSSVVLRKLFPSHSYKTLLALLPPIKLYRRLLRAHRHKLSPEKRVLGDEYVKAEFRRHRDIENPLYIVGFLKEWQLYAQAVEGETWKDEKLEEGKLEKMSDQQIAQLYELMRAIKRGDTDAGGEGEGRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9H1</accession>
    <name>D5G9H1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00003399001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IUNH family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430061">
        <property type="protein sequence ID" value="CAZ81164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836973.1">
        <property type="nucleotide sequence ID" value="XM_002836927.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9H1"/>
    <dbReference type="FunCoup" id="D5G9H1">
        <property type="interactions" value="174"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G9H1"/>
    <dbReference type="GeneID" id="9182915"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003399001"/>
    <dbReference type="eggNOG" id="KOG2938">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036838_2_0_1"/>
    <dbReference type="InParanoid" id="D5G9H1"/>
    <dbReference type="OMA" id="NVEHIHA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070635">
        <property type="term" value="F:nicotinamide riboside hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070636">
        <property type="term" value="F:nicotinic acid riboside hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008477">
        <property type="term" value="F:purine nucleosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045437">
        <property type="term" value="F:uridine nucleosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006216">
        <property type="term" value="P:cytidine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034355">
        <property type="term" value="P:NAD+ biosynthetic process via the salvage pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019358">
        <property type="term" value="P:nicotinate nucleotide salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006152">
        <property type="term" value="P:purine nucleoside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008655">
        <property type="term" value="P:pyrimidine-containing compound salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006218">
        <property type="term" value="P:uridine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.245.10">
        <property type="entry name" value="Ribonucleoside hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001910">
        <property type="entry name" value="Inosine/uridine_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023186">
        <property type="entry name" value="IUNH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036452">
        <property type="entry name" value="Ribo_hydro-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12304">
        <property type="entry name" value="INOSINE-URIDINE PREFERRING NUCLEOSIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12304:SF4">
        <property type="entry name" value="URIDINE NUCLEOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01156">
        <property type="entry name" value="IU_nuc_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53590">
        <property type="entry name" value="Nucleoside hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inosine/uridine-preferring nucleoside hydrolase" evidence="4">
        <location>
            <begin position="8"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ81164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="341" mass="36810" checksum="66FE03EF2478AD96" modified="2010-06-15" version="1">MAATKTRIWLDCDPGHDVSEHPNLELVGISTVHGNSSMDNVTSNALSLLTAIGKTDVPVYAGSRKPFMRPAVHAPDIHGDSGIDGTTLLPESKVAMKSGNTILAIYEAIMKTPFQTCAVVATGTLTNVALLFAAFPDVVGHIRCVSIMGGAFGGREDARGNITKWAEFNIYCDPESARSIFSNPELSGRITLVPLDLTHTVLATSDVLTALMTTSHPTTKLRKMLHDLLTFFSKTYAEVFNITAGPPLHDPLAVAATIPGNEIEWDMEEVQVEVVCHGEQIGQTIKTPRDQAHGGVRGEELGKGGAEPVTWVKVPRSVAVDSFWKVMMSCVEKADGRYNWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9J7</accession>
    <name>D5G9J7_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ATP synthase subunit 5, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00003355001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATPase delta chain family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430062">
        <property type="protein sequence ID" value="CAZ81190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836999.1">
        <property type="nucleotide sequence ID" value="XM_002836953.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9J7"/>
    <dbReference type="FunCoup" id="D5G9J7">
        <property type="interactions" value="656"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G9J7"/>
    <dbReference type="GeneID" id="9185327"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003355001"/>
    <dbReference type="eggNOG" id="KOG1662">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085114_0_0_1"/>
    <dbReference type="InParanoid" id="D5G9J7"/>
    <dbReference type="OMA" id="MVDNIQD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.20">
        <property type="entry name" value="N-terminal domain of the delta subunit of the F1F0-ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01416">
        <property type="entry name" value="ATP_synth_delta_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026015">
        <property type="entry name" value="ATP_synth_OSCP/delta_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020781">
        <property type="entry name" value="ATPase_OSCP/d_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000711">
        <property type="entry name" value="ATPase_OSCP/dsu"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01145">
        <property type="entry name" value="ATP_synt_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11910">
        <property type="entry name" value="ATP SYNTHASE DELTA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00213">
        <property type="entry name" value="OSCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00125">
        <property type="entry name" value="ATPASEDELTA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47928">
        <property type="entry name" value="N-terminal domain of the delta subunit of the F1F0-ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00389">
        <property type="entry name" value="ATPASE_DELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ81190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="216" mass="23103" checksum="6EAAEDEE6B24E743" modified="2010-06-15" version="1">MFPIRSASIARAIVPRVGRAYAAASTSTRPPVQLFGVDGTYASALYTASAKTRSLDSTEKSLQTLKGILQKDPKLETIISSPTLNAGDKSQIIAEIARPIASDKTVKNLLEVLAENNRLGLLRGVIDKYSTLMSAHKGVVEAVITSASRLDPRTVSRLETAISKSQYVGAGKTLEVVNKVNPEIRGGLVVEIGERTIDLSVSAKMSRLNKLLTDAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9S4</accession>
    <name>D5G9S4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00005050001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430064">
        <property type="protein sequence ID" value="CAZ81267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837076.1">
        <property type="nucleotide sequence ID" value="XM_002837030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9S4"/>
    <dbReference type="STRING" id="656061.D5G9S4"/>
    <dbReference type="GeneID" id="9188510"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005050001"/>
    <dbReference type="eggNOG" id="ENOG502QWE4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007933_3_0_1"/>
    <dbReference type="InParanoid" id="D5G9S4"/>
    <dbReference type="OMA" id="PGMTGHN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.420.10">
        <property type="entry name" value="Maltose phosphorylase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34987:SF6">
        <property type="entry name" value="ALPHA-L-RHAMNOSIDASE SIX-HAIRPIN GLYCOSIDASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34987">
        <property type="entry name" value="C, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G02880)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072608" evidence="1">
        <location>
            <begin position="23"/>
            <end position="738"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="738" mass="81861" checksum="6704E46E7AE5B80C" modified="2010-06-15" version="1">MPWLTLLRSSLLLLLAAAVCESQDTPIFPGPWERYIQAPESRQVRPRKVYYTEGTVSAATDSSGVTVLKGQGALVTYEFAQNIAGRLFFEVDSATTSKHQSIGIAFSESPRFIGRRTDGTGDRIEYDLTHFQPLTSGLNSLDQEHVRGAFKYVTLFIPHPRDPKAGQALPHIASLTTKSIAADLSDLAQMDFGTRGGQQPLLPVESVGFKDVWVKYSAFPSHENPRRYTGYFYSNDNLLNRIWYAGAYTLQITTIPPKEGGTLIDLNKVVDHNRAPHGTWYSNFTISNGTSVTTDGAKRDRMVYAGDMTLAAPGIAVSTYTEYSHEEGGKGSGMIRLPYAGPPMGFRFEFSDTYHMHALLGVYQYVLYSGDFDYLKHLWWKYVQAIEYSTSKILKHTGLMYVTSGNDWLRPGMGRENIEAQGILLKTLFGSVKLAEWLDEDGYKFKDVKGRDLKQLITLWEGYMAGIKKGVMAPEKLWNGTVGLFCDNPEDRRDIFPQDGNSWAVATGLVTGGIAHRISRELKNRWGTYGAPAVELPNTISPFASGFELQAHTQIGNYQNALDLMRRQWGYMLDGPGFTNSTLVEGFRMDGDIQYPAYWSPTRNSHAHGWSAGPTSVLTDSILGIKLLSPLGKTWSITPQPGDLTHVEGGFQTSLGKFVVNYDRTAEEEVLEVETPEGSFGTLNWGDLVGGMRMEAGGKWRLTKSRDGKIRGGKVGTRLVHQDGLGQGRPQDAVTEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5GA21</accession>
    <name>D5GA21_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00003504001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430070">
        <property type="protein sequence ID" value="CAZ81364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837173.1">
        <property type="nucleotide sequence ID" value="XM_002837127.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GA21"/>
    <dbReference type="GeneID" id="9187062"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003504001"/>
    <dbReference type="eggNOG" id="ENOG502QXVT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017895_1_0_1"/>
    <dbReference type="InParanoid" id="D5GA21"/>
    <dbReference type="OMA" id="YDCQRHT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF120">
        <property type="entry name" value="KRUPPEL-LIKE FACTOR 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="5">
        <location>
            <begin position="288"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="5">
        <location>
            <begin position="313"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="5">
        <location>
            <begin position="345"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="5">
        <location>
            <begin position="406"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="131"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="374"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="450"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="133"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="180"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="237"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="469"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="491"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ81364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="611" mass="66150" checksum="C7DFBCA5AEE945DB" modified="2010-06-15" version="1">MAASSSSQAPTSASIPTKDTTGMADFVLFDDDPLYQEYRPRYDNGGFPQSGVDSYVAMLEQPSEDHQPKLYMAGLQPGSTSFETYPPYAYDEVPLTMGYEPGPAPGVLIHPPTLPYSPHGNITASLEYRTPISPATSAGGPEGSSAGSEAGSTGSPYPQQIDDPFSSVYVDIDPSLIMPSPSQQFEDPCSAASSRGSPSWHSAHSPPPTSNPSSPELGKRSGGRLSPLRQYRTAPYPSRRLSVTSQSSSRSYGSPSGPSELEDMYPAAPGDIPRRAPSSNGSGTGKDTLCPECKKSFRDLKAHQLTHQLERPVKCPIATCEYSKKGFARKYDCQRHTLTHYKGTMVCGFCPGSGSAAEKSFNRADVFKRHLMSVHNVEQTPPNSRKRPPTKTSPAEDRYAGAYVTGKCSTCSITFATAQQFYEHLDECVLSKVVQEEPAAAFNEMNLDSVKLSDMDPPSSNPADPTSSDSEDEDEDEKDTTFNPATNKSAAKIRIKKGTIKHSGSGAGSTQSAKTKTIRRKRKNFPAGWGCQPEQMVTKRRCLMVFDGPKLLCRDEMMMSNEWEVRAQLGGSGGSDAYISDLDFWTLRRAEAFLDAAPSRNPQGAAVGEVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAC5</accession>
    <name>D5GAC5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00005236001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the importin beta family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430075">
        <property type="protein sequence ID" value="CAZ81479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837288.1">
        <property type="nucleotide sequence ID" value="XM_002837242.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAC5"/>
    <dbReference type="FunCoup" id="D5GAC5">
        <property type="interactions" value="212"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GAC5"/>
    <dbReference type="GeneID" id="9185761"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005236001"/>
    <dbReference type="eggNOG" id="KOG2022">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005271_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAC5"/>
    <dbReference type="OMA" id="CLASIGK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051345">
        <property type="entry name" value="Importin_beta-like_NTR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040520">
        <property type="entry name" value="Importin_rep_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057942">
        <property type="entry name" value="TPR_TNPO3_IPO13_3rd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12363:SF33">
        <property type="entry name" value="IMPORTIN-13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12363">
        <property type="entry name" value="TRANSPORTIN 3 AND IMPORTIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18806">
        <property type="entry name" value="Importin_rep_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24140">
        <property type="entry name" value="TPR_TNPO3_IPO13_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ81479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="647" mass="71621" checksum="32F39B49D4F3BEC4" modified="2010-06-15" version="1">MLHGMVKVPGYPMAEEEISGTTFEFWSSLAEFLLDPENVAESDIAPLMAAGKKEIMQAIEEFWTKIQIPPHTESLKWTKDFRDGFTSFRKDVADLVEVAYGILGLELFDRLISQVIIALGNSQVGGAVAWEQIEASLFCLNSLSDCLGDEPEEDESLKVLFQSKLFNTLADFGNQIPLKVRQTAVHMIGSYAVFFERWANFLPTVLNFLFVAISTPALARNASKSISSLCSSCRSTLTSELSVFLYQYELFSATPTADDIAKERVLCAISYVIQALPSESQKLDSISRMLEFVDKDVQQFLTILHRHPEAAKELGVSTLQCLVAIGKGLQAPDDIPIILAGEDGGQVPPSFWEQERGLAVQTRILHILQTLVGSLTEDGEIIDAACAVFRTGFAETTPGPFVFPAAVVTKFLLERANCGVRVETVLSTASTMVSSHSLDGSPDISTEVGFFLELILGLVERLQNPQEDPEVSQGLVEFLNRLFSRYITVIACYQPKERIEMLLWFVMNALQVRETLVKKAACTFWASLVSFAADDRPELQARINSFVHMCGPNLAEKLIWGIGGGASRSEVDSLTEPLKKMIARQVKAKTWLSASLLRNGFPSPNLSEKDKTIFLNKIMTLRGKRGTNQIAKEFWLSSRGSDFAYTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAH2</accession>
    <name>D5GAH2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00003582001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430077">
        <property type="protein sequence ID" value="CAZ81515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837324.1">
        <property type="nucleotide sequence ID" value="XM_002837278.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAH2"/>
    <dbReference type="GeneID" id="9187455"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003582001"/>
    <dbReference type="eggNOG" id="ENOG502QPU8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009024_1_0_1"/>
    <dbReference type="InParanoid" id="D5GAH2"/>
    <dbReference type="OMA" id="NEFIPPW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901136">
        <property type="term" value="P:carbohydrate derivative catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041036">
        <property type="entry name" value="GH5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052066">
        <property type="entry name" value="Glycosphingolipid_Hydrolases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31308">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31308:SF5">
        <property type="entry name" value="ERGOSTERYL-BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18564">
        <property type="entry name" value="Glyco_hydro_5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="4">
        <location>
            <begin position="92"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 5 C-terminal" evidence="5">
        <location>
            <begin position="646"/>
            <end position="714"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF18564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ81515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="772" mass="86087" checksum="68EE5F80656B20E0" modified="2010-06-15" version="1">MNSSYSPRPVVIPTSLPPSTWFDPSSISAHPSANAFTDDHRRTILLRGINVSGDAKFPQNNPLARTPEFYNVDNVSYVGKPFGSVDEAKVWWSRLKGWGVGVVRMVVCWEALEPREMGKYDEEYIDFLRVILDKANEVGLLVFIDGHQDVWSRFTGGSGAPLWTFHLAGLDPSVFPTTYSAALDSADTSSHHSTPSGRLWPTNYSKFGPATMFTLFFAGEIFAPSAIYEGPIQEFQAKNIGQVLRTAYVRAYSHLASRLKDLPNILGFDAMNEPHPGYISLPSLHRFNENTDLHLGYMPNALEGMSLAAGIPTSIGYFSRSWPHPSKRTKSEVLNKECKSAWLPGRRDIWQEEGVFTVDDGKVKLRSRGDSYFTTNPRTGDKIDFEKDCYVPFLRSFMQSVREAVKGYSAGRWMFIEPIPNLEPPVWNGDGIKKTAEQNICYSPHWYDIRVLYEKGLWYGVTFDVLSLAAGSRNLFKHTYFGREGLTANYTTNFARFASHLSTFRPSPSSHTPILIGETGIPFDMNGQNSYVTGDVHMQLSQLDSIFRGMEKSLLNWTVWNLTLSHSTTATDMTTPTANETGAVIQSGDGWNSEDFSLVSLYNPNTVEVPLEPSHVGPTNGGVPLTRARSFGGLYLGGRLLPALLRPYPAKTAGKLISSHFLMDSKNFHMEFVGNASGKEIERTTEIFIPAYHFSGQRCLVTISDGNEQEWVVNKPSMDAKIEGGVEWRWREEEQGMTVVHTTSGGLITIEVRVLESVESSSFIENLVGGFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAL2</accession>
    <name>D5GAL2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00003668001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430079">
        <property type="protein sequence ID" value="CAZ81555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837364.1">
        <property type="nucleotide sequence ID" value="XM_002837318.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAL2"/>
    <dbReference type="GeneID" id="9187495"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003668001"/>
    <dbReference type="eggNOG" id="ENOG502QPK1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_46_1_1"/>
    <dbReference type="InParanoid" id="D5GAL2"/>
    <dbReference type="OMA" id="YIMIAFS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046943">
        <property type="term" value="F:carboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17316">
        <property type="entry name" value="MFS_SV2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508:SF9">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORT PROTEIN (AFU_ORTHOLOGUE AFUA_2G09450)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="85"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="148"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="206"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="243"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="271"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="1"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="417"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ81555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="443" mass="48720" checksum="6522B9618E1586E1" modified="2010-06-15" version="1">MDGYDFHTVSLSVSRLATFYNQHREHISTSITLTLLFRSVGAATFGIAGDLYGRKYPMIVNLVIIAALQLATAYCATFSQFLGVRALFGIGMGGIWGLAASMGLENMPMEARGLFSGILQQGYALGYLIAAVFNLYVVPNSSHSWKALFFIGAGLTVAVAIARLFFPESKQFIEAKRSGENATGRKKVKLFWKDGKNIMKEYWKRAIYAVVMMALFNFMSHTSQDMYPTYMQQTKGFSPQMSSKATIIAKTGALVGGTICGYYSQFFGRRATIMVATFCGACLIPMWVLPSSWGTLTAGAFLIQFMVQGAWGVVPIHLQELSPPQFRSSFPGIAYQLGNMISAPAAQISSVISEGWIIHVNGEERPDYARTQAAMMAVIFSLLCFWIACGSEVRGSHFELAAAAGGDDSHNDKLRNLENLEMRDPFDTPKEDVEIAENKHSKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GB53</accession>
    <name>D5GB53_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00005454001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430086">
        <property type="protein sequence ID" value="CAZ81746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837555.1">
        <property type="nucleotide sequence ID" value="XM_002837509.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GB53"/>
    <dbReference type="GeneID" id="9181457"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005454001"/>
    <dbReference type="HOGENOM" id="CLU_3399658_0_0_1"/>
    <dbReference type="InParanoid" id="D5GB53"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ81746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="31" mass="3647" checksum="F338D3843043E870" modified="2010-06-15" version="1">MYHSFSKTGMLSKAMIPRVAYRYLTSTYMVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBV0</accession>
    <name>D5GBV0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutaredoxin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00005596001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutaredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ81950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837759.1">
        <property type="nucleotide sequence ID" value="XM_002837713.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBV0"/>
    <dbReference type="GeneID" id="9182987"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005596001"/>
    <dbReference type="eggNOG" id="ENOG502SC8X">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125054_0_2_1"/>
    <dbReference type="InParanoid" id="D5GBV0"/>
    <dbReference type="OMA" id="SDVWDKR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008554">
        <property type="entry name" value="Glutaredoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052565">
        <property type="entry name" value="Glutaredoxin-like_YDR286C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33558">
        <property type="entry name" value="GLUTAREDOXIN-LIKE PROTEIN C5ORF63 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33558:SF1">
        <property type="entry name" value="GLUTAREDOXIN-LIKE PROTEIN C5ORF63 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05768">
        <property type="entry name" value="Glrx-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0249">Electron transport</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="121" mass="13973" checksum="C89F39452B7FEA93" modified="2010-06-15" version="1">MHQTLVLFLRRLPIHITLYSRATCSLCDTARTELSQAWEKRPFEYAEVDVMKDDKWRVYEFDVPVIHVSRTIGEKGIEEGEKTRKLMHKFKAAEVVGVMEKVLSEGGVERKEGGVEDGRSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBY3</accession>
    <name>D5GBY3_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Orotate phosphoribosyltransferase</fullName>
            <ecNumber evidence="5">2.4.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00005642001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate</text>
            <dbReference type="Rhea" id="RHEA:10380"/>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57538"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.10" evidence="5"/>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ81983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837792.1">
        <property type="nucleotide sequence ID" value="XM_002837746.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBY3"/>
    <dbReference type="FunCoup" id="D5GBY3">
        <property type="interactions" value="197"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GBY3"/>
    <dbReference type="GeneID" id="9185418"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005642001"/>
    <dbReference type="eggNOG" id="KOG1377">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074878_0_0_1"/>
    <dbReference type="InParanoid" id="D5GBY3"/>
    <dbReference type="OMA" id="SPFFMNA"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00119"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004588">
        <property type="term" value="F:orotate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046132">
        <property type="term" value="P:pyrimidine ribonucleoside biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000008">
        <property type="entry name" value="Orotate phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01208">
        <property type="entry name" value="PyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023031">
        <property type="entry name" value="OPRT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004467">
        <property type="entry name" value="Or_phspho_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00336">
        <property type="entry name" value="pyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46683">
        <property type="entry name" value="OROTATE PHOSPHORIBOSYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46683:SF1">
        <property type="entry name" value="OROTATE PHOSPHORIBOSYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="11">
        <location>
            <begin position="75"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00014769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ81983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="232" mass="24917" checksum="191187F8926D85DA" modified="2010-06-15" version="1">MSSKSPETLPLFKQTFLQTLLQSKILTFGTFTLKSGRESPYFFNAGLFNTGSLLTSLGAAYANTIHTSASLPPFDVVFGPAYKGIPLATITLAKLYELAPEKYAGAGYAFDRKEVKDHGEGGRMVGMGLRGKRVLVVDDVITAGMAIRGAVSAIEEAGGMVIGIVVALDRMERIGGVGAEDRKGSAIMEIKKEMGVEVLAILNLDELIVGVTDEGSRERMLEYRRKYGAIGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBZ7</accession>
    <name>D5GBZ7_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Importin subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00005727001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the importin alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430098">
        <property type="protein sequence ID" value="CAZ82040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837849.1">
        <property type="nucleotide sequence ID" value="XM_002837803.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBZ7"/>
    <dbReference type="FunCoup" id="D5GBZ7">
        <property type="interactions" value="1039"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GBZ7"/>
    <dbReference type="GeneID" id="9181309"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005727001"/>
    <dbReference type="eggNOG" id="KOG0166">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018084_6_0_1"/>
    <dbReference type="InParanoid" id="D5GBZ7"/>
    <dbReference type="OMA" id="EMIQMLY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061608">
        <property type="term" value="F:nuclear import signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.690:FF:000003">
        <property type="entry name" value="Importin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000021">
        <property type="entry name" value="Importin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.690">
        <property type="entry name" value="Importin-alpha, importin-beta-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032413">
        <property type="entry name" value="Arm_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002652">
        <property type="entry name" value="Importin-a_IBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036975">
        <property type="entry name" value="Importin-a_IBB_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024931">
        <property type="entry name" value="Importin_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23316">
        <property type="entry name" value="IMPORTIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16186">
        <property type="entry name" value="Arm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01749">
        <property type="entry name" value="IBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005673">
        <property type="entry name" value="Importin_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51214">
        <property type="entry name" value="IBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 6 8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="IBB" evidence="10">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="7">
        <location>
            <begin position="118"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="7">
        <location>
            <begin position="160"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="7">
        <location>
            <begin position="202"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="7">
        <location>
            <begin position="329"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="511"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="19"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="514"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00071843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR005673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ82040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="545" mass="59815" checksum="C6FBC9A04583E3E1" modified="2010-06-15" version="1">MADRYIPEHRRNFKAKANFKPDELRRRREEQQVEIRKQKREENLAKRRNMAVTSPAPGAGVDSDEEGGTESQLNEDLPSMCAGVFSESIDQQIQATTRFRKLLSKERNPPIEKVIECGVVSRFVEFLRSPHTLVQFEAAWALTNIASGSASQTQVVIEAGAVPIFVDLLSSHEPDVREQAVWALGNIAGDSPQCRDFVLSAGALPPLLSLLGDSRKLSMLRNATWTLSNFCRGKTPQPDWPTILPALPVLAKLVYSLDDEVLIDACWAISYLSDGSNDKIQAVIENGIPRRLVELLMHSSTSVQTPALRSVGNIVTGDDVQTQVIINCGALPALLALLGSPKDGIRKEACWTISNITAGNSTQIQAVIDANIIPPLINLLSHGDFKTRKEACWAISNATSGGLQKPEQIRYLVSQGCIRPLCDLLGCMDNKIIQVALDGLENILKVGDMDKNAETDNMNRYAMFIEEAGGMERIHAAQNNANEEIYMKAYNIIEKYFSDEDDETGAEIQGLAPQPQQDGQAGTFSFGAQPQQGGFNFANGDSMDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCX2</accession>
    <name>D5GCX2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00000849001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430128">
        <property type="protein sequence ID" value="CAZ82365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838174.1">
        <property type="nucleotide sequence ID" value="XM_002838128.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCX2"/>
    <dbReference type="GeneID" id="9183828"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000849001"/>
    <dbReference type="HOGENOM" id="CLU_2374301_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCX2"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="10"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="95" mass="10732" checksum="F83F7D2FD8BC86E1" modified="2010-06-15" version="1">MAGLLFPLLSSSSLIFYPPPPPLPKHSSRRSSKKGKYPPTYQNTKNQPSLLCSPPSLFISHQESFSLRAPTNAFRDRNCQLYKSSHIRSSRVFPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>D5GD75</accession>
    <name>D5GD75_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">leucine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Leucyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">GSTUM_00006079001</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>tRNA(Leu) + L-leucine + ATP = L-leucyl-tRNA(Leu) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11688"/>
            <dbReference type="Rhea" id="RHEA-COMP:9613"/>
            <dbReference type="Rhea" id="RHEA-COMP:9622"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78494"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.4" evidence="3"/>
    <dbReference type="EMBL" id="FN430142">
        <property type="protein sequence ID" value="CAZ82468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838277.1">
        <property type="nucleotide sequence ID" value="XM_002838231.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GD75"/>
    <dbReference type="FunCoup" id="D5GD75">
        <property type="interactions" value="481"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GD75"/>
    <dbReference type="GeneID" id="9183932"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006079001"/>
    <dbReference type="eggNOG" id="KOG0435">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004427_0_0_1"/>
    <dbReference type="InParanoid" id="D5GD75"/>
    <dbReference type="OMA" id="DDVDWAD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004823">
        <property type="term" value="F:leucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006429">
        <property type="term" value="P:leucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00812">
        <property type="entry name" value="LeuRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000003">
        <property type="entry name" value="Leucine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000100">
        <property type="entry name" value="probable leucine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.590">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002302">
        <property type="entry name" value="Leu-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025709">
        <property type="entry name" value="Leu_tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015413">
        <property type="entry name" value="Methionyl/Leucyl_tRNA_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00396">
        <property type="entry name" value="leuS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43740:SF2">
        <property type="entry name" value="LEUCINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43740">
        <property type="entry name" value="LEUCYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13603">
        <property type="entry name" value="tRNA-synt_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09334">
        <property type="entry name" value="tRNA-synt_1g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00985">
        <property type="entry name" value="TRNASYNTHLEU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methionyl/Leucyl tRNA synthetase" evidence="14">
        <location>
            <begin position="2"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Leucyl-tRNA synthetase editing" evidence="15">
        <location>
            <begin position="185"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="12">
        <location>
            <begin position="388"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="13">
        <location>
            <begin position="655"/>
            <end position="737"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF09334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF13603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAZ82468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="838" mass="95070" checksum="2C973A108B86B406" modified="2010-06-15" version="1">MFPYPSGVLHMGHLRVYTISDVLARFQRMRGKRVIHPMGWDAFGLPAENAAIEGGVSAKKWTEKNIDSMKEQFSLMNVGFDWDREIRTCSPDYYVHTQRLFLKLYEHGLAYRKESQVNWDPVDETVLANEQVLDNGKSWRSGAVVERRTLPQWFFKITAYKKALLEDLDVLGPDWPDQVKSMQANWIGLSRGVKVKFPLTAGKTDLGGYTSLEVYTTRLDTLLGVQYLAISSIHPLALKSIETDKYLQKFLLIHGTDWNTRSKKGELIKGFRALNPLTNEEIPVYVANYVVDGTGTSAVMGVPGHDARDNEFWKKRGPQEVPAKVVVRQSRYDRKKNLRPEDGGVFEDLGTLNENCGRYEGMSSAHAQKVFLTELRRKGLGKAHVNSKLEDWLISRQRYWGTPIPMVHCESCGIVPVKEEDLPVVLPDIEKLSPKGGNPLVSMKEWVETPCPKCGGEARRETDTMDTFVDSSWYFMRYVDPHNKELPFSPEKANELLPVDAYIGGIEHAILHLLYARFISKFLRKIGMWPLGSDSENQGEPFKRLVTQGMVSADGASPRVSWEKMSKSKFNGTDPRECISRWGADATRAHLLFLGPVQAELRWDEDRIIGMHRWFDKLWRHTSMLAEKLADPGFPILPNLIARPVTTTSIPPEDHDLFSMLQETNRNSSFALSKTLALNTVVSNLIKLTNTLTSPPAETQPTLAMTYRVTNELLRMLAPIAPGFAEECWARLHTHVPSMAGTRVLETTWPWFVLQGERLGGTTTCSVHIDGRFRFKVTLERERLMRKLGEGEQYARDIIANKETAGKWLKEKEIEKVIVSSNGKVVSLLTRPVTRTPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDA4</accession>
    <name>D5GDA4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00006122001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0220 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430142">
        <property type="protein sequence ID" value="CAZ82497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838306.1">
        <property type="nucleotide sequence ID" value="XM_002838260.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDA4"/>
    <dbReference type="FunCoup" id="D5GDA4">
        <property type="interactions" value="192"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GDA4"/>
    <dbReference type="GeneID" id="9183961"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006122001"/>
    <dbReference type="eggNOG" id="KOG3393">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096876_0_0_1"/>
    <dbReference type="InParanoid" id="D5GDA4"/>
    <dbReference type="OMA" id="VHITFVD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034424">
        <property type="term" value="C:Vps55/Vps68 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007919">
        <property type="entry name" value="UPF0220"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13180">
        <property type="entry name" value="SMALL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05255">
        <property type="entry name" value="UPF0220"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="96"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="132"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ82497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="168" mass="18030" checksum="1E4430063F06EC19" modified="2010-06-15" version="1">MSRLWRFPVPKLPSSAVLRTAGVYTSGALFSLGFFLLLDASVYSKHANGGDVHITIVDWIPLICSILGMLVINSIERSRLVADSYSYSGNGVAWKAKLVLFMGFALMAGGLAGAATVLIMKYVVPAYPWPTLGFGVAGVVGSACVMLSSVVLWVSQNIEEDYTYNLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDG6</accession>
    <name>D5GDG6_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">glutathione gamma-glutamylcysteinyltransferase</fullName>
            <ecNumber evidence="1">2.3.2.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00001002001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.2.15" evidence="1"/>
    <dbReference type="EMBL" id="FN430143">
        <property type="protein sequence ID" value="CAZ82558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838367.1">
        <property type="nucleotide sequence ID" value="XM_002838321.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDG6"/>
    <dbReference type="STRING" id="656061.D5GDG6"/>
    <dbReference type="GeneID" id="9184017"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001002001"/>
    <dbReference type="eggNOG" id="KOG0632">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046059_1_0_1"/>
    <dbReference type="InParanoid" id="D5GDG6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016756">
        <property type="term" value="F:glutathione gamma-glutamylcysteinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046938">
        <property type="term" value="P:phytochelatin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010038">
        <property type="term" value="P:response to metal ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.30:FF:000001">
        <property type="entry name" value="Glutathione gamma-glutamylcysteinyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.30">
        <property type="entry name" value="Phytochelatin synthase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040409">
        <property type="entry name" value="PCS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007719">
        <property type="entry name" value="PCS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038156">
        <property type="entry name" value="PCS_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33447">
        <property type="entry name" value="GLUTATHIONE GAMMA-GLUTAMYLCYSTEINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05023">
        <property type="entry name" value="Phytochelatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51443">
        <property type="entry name" value="PCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0104">Cadmium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Peptidase C83" evidence="5">
        <location>
            <begin position="5"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ82558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="416" mass="46212" checksum="574F7C07BA4E9350" modified="2010-06-15" version="1">MATRQSQTSFYMRELPKEHLIGYDTPEGKRLFAKALAEGGLEAFFPLSQQFLTVYCGIGTLCMILNALKVDPTVTWRKPWRWFTQEMLDCCQPLEVVKEKGITLAEFSCLAKCNGLEAVTRFADSTSFEAFHQAVKVCTFSADQLMAVSYSRASLGQTGLGHFSPVGGYTSENGGMVLILDVARFKYPSYWVPIRMLFEALIPKDPVTNQPRGYSMLRRHHTDVFSPNPIGSLLRLNATKSTWPPLFKPFYIAAQSSHTYTSLLQTLTHLLSGIPTPVTSRSPEDPPNAPPTLQCGSAPALLANIENKLIYERHIDSFLAHLEAKSSMYAGLDGEFTRMEKTVFLLSLFGMQAFLGVLAPPVKKAVLEAAGKDLEDEWVRREVMAVRRQMESVEKCCWEEKECSCAAAAAGAVAPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDJ6</accession>
    <name>D5GDJ6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="13">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00001051001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430147">
        <property type="protein sequence ID" value="CAZ82589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838398.1">
        <property type="nucleotide sequence ID" value="XM_002838352.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDJ6"/>
    <dbReference type="STRING" id="656061.D5GDJ6"/>
    <dbReference type="GeneID" id="9182539"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001051001"/>
    <dbReference type="eggNOG" id="KOG0064">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007587_5_0_1"/>
    <dbReference type="InParanoid" id="D5GDJ6"/>
    <dbReference type="OMA" id="DIQAGHF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005324">
        <property type="term" value="F:long-chain fatty acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015910">
        <property type="term" value="P:long-chain fatty acid import into peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042760">
        <property type="term" value="P:very long-chain fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03223">
        <property type="entry name" value="ABCD_peroxisomal_ALDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000636">
        <property type="entry name" value="ATP-binding cassette sub-family D member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050835">
        <property type="entry name" value="ABC_transporter_sub-D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384">
        <property type="entry name" value="ATP-BINDING CASSETTE, SUB-FAMILY D MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384:SF69">
        <property type="entry name" value="PEROXISOMAL LONG-CHAIN FATTY ACID IMPORT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06472">
        <property type="entry name" value="ABC_membrane_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="109"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="147"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="343"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="12">
        <location>
            <begin position="114"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="458"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="57"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ82589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="708" mass="80477" checksum="02BB64808977F344" modified="2010-06-15" version="1">MAAQSKLPLPSDRSLTYLLSHLSSLYVKHRKKISRGVYLTLFIALVNRVRNAINEQKAAAARQGDRPRPAPETPSAKRKKAELNREFFQNLFKLLKIVIPGVRSKELRLLVSHTFFLIVRTLISLYVAELDGKLVSALVRGKGKDFLLGIVWWMVVAVPATFTNSMLSYHQCKLALQYRTRLTQYIHSKYLSQMTFYTLGNLDDRIKNADQLITVDVSKFSNSLAELYSNLAKPVLDMMIYNYQLSRNVGGEGLFAMALLVQVSANVMRVLTPPFGRYVADEARLEGEYRFSHSRLIDNSEEIALYGGHDAEKTTLDKGYFTLIKHVNYILRRRLYHGMMEDFVIKYFWGALGLMLCSVPVFFKVPGARGNSLGDRTESFVTNRRLLLSSSDAFGRVMFSYKEITELAGYTSRVSTLLNVIEDIQNGHFEKKLVSSAGTEENAAVLRGRGKVTTGPDIEFTDVPIVSPNGDVLLKKLSFTVKSDEHLLIVGPNGCGKSSLFRIMGGLWPVYGGTVRKPPSEEIFYIPQRPYLSRGTLRQQIIYPDSKLEQVRRGVTDEALYEILRVVEIENLVEKEGGWEAEKEWRDVWSGGIQQRVAMARLFYHAPKYAILDECTSSQDLDTERIMYETAKSLGITLITVSHRRSLWKYHTNILQFDGQGGYVFTKLDAEKRLKLEDEKEEIEIQLRAVPDVEKRVTELEAVVAERD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDN7</accession>
    <name>D5GDN7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00006203001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430153">
        <property type="protein sequence ID" value="CAZ82630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838439.1">
        <property type="nucleotide sequence ID" value="XM_002838393.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDN7"/>
    <dbReference type="STRING" id="656061.D5GDN7"/>
    <dbReference type="GeneID" id="9181635"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006203001"/>
    <dbReference type="eggNOG" id="ENOG502QS7Z">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020266_0_0_1"/>
    <dbReference type="InParanoid" id="D5GDN7"/>
    <dbReference type="OMA" id="LLWRHHC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.390">
        <property type="entry name" value="Hemimethylated DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011722">
        <property type="entry name" value="Hemimethylated_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036623">
        <property type="entry name" value="Hemimethylated_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032698">
        <property type="entry name" value="SirB1_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02097">
        <property type="entry name" value="yccV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31350:SF27">
        <property type="entry name" value="HEMIMETHYLATED DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31350">
        <property type="entry name" value="SI:DKEY-261L7.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13369">
        <property type="entry name" value="Transglut_core2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08755">
        <property type="entry name" value="YccV-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00992">
        <property type="entry name" value="YccV-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141255">
        <property type="entry name" value="YccV-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="4"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="567" mass="64781" checksum="0A4B82557EFEF3A9" modified="2010-06-15" version="1">MTKDSILLSLPEEVIENTLKFIPDDDSALLDISLVCKKLYRLSNSPLEWRTRCRRFRYWEPRHEYQKKVKHPLPAEVDWRSLFVERIYSYKRTTMLLNEIISSPVDRINKFNKIADIGCDAKDCLAEHANASDDVEDVLARRWYAAAAIHYIHRRQAIMGWKKLASGEDISLEYALGCFELFILKTPEGDIDDISGILDELAARFRSETANFADMSTKAKALALCTYMNREGFRGASYESYRALKNAFIGVSLRTDRTNLPLTATAIYCSLATRVGLAAYPCSFPYHVLAIVRNEDGNYIYLDPFRGEGSDLPVQGLEHQLSELGVGATKDEFLCPSPTTDIVLRTSRNILESLRRAPGPPDIQQEAVEIDRHSALYAALTASALLGPRITLNVVQHMARRIQDEYSMDVQFFEEDIVPKLEDRNARELLLEKCGELRAEDSAQRPVSRRENSDGNDVRYRVGTVFVHKRYPFQGVVFGWSRTCAPREGEEWMQTMGVDTLSRGRHQPFYLALVMDGSVRYVAEENIVVSQTDPVESLKKLAGKYFKRYSRAERMFISNIRDEYPDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GE82</accession>
    <name>D5GE82_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00006418001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430164">
        <property type="protein sequence ID" value="CAZ82825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838634.1">
        <property type="nucleotide sequence ID" value="XM_002838588.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GE82"/>
    <dbReference type="GeneID" id="9185481"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006418001"/>
    <dbReference type="HOGENOM" id="CLU_2544253_0_0_1"/>
    <dbReference type="InParanoid" id="D5GE82"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="83" mass="9424" checksum="F80F50D607A0E02F" modified="2010-06-15" version="1">MVNRQQHLAATDAIGCHSEPRQAAITSQDSNPVRDNMRMERVGRKGSHELPITEYQIIPCLWVRSSQTLGTLCPLVRPKHYMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5GE86</accession>
    <name>D5GE86_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">5'-deoxynucleotidase</fullName>
            <ecNumber evidence="8">3.1.3.89</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00006427001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the dephosphorylation of the nucleoside 5'-monophosphates deoxyadenosine monophosphate (dAMP), deoxycytidine monophosphate (dCMP), deoxyguanosine monophosphate (dGMP) and deoxythymidine monophosphate (dTMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2'-deoxyribonucleoside 5'-phosphate + H2O = a 2'-deoxyribonucleoside + phosphate</text>
            <dbReference type="Rhea" id="RHEA:36167"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18274"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:65317"/>
            <dbReference type="EC" id="3.1.3.89"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the HDDC2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.89" evidence="8"/>
    <dbReference type="EMBL" id="FN430164">
        <property type="protein sequence ID" value="CAZ82829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838638.1">
        <property type="nucleotide sequence ID" value="XM_002838592.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GE86"/>
    <dbReference type="FunCoup" id="D5GE86">
        <property type="interactions" value="468"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GE86"/>
    <dbReference type="GeneID" id="9185485"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006427001"/>
    <dbReference type="eggNOG" id="KOG3197">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039453_2_0_1"/>
    <dbReference type="InParanoid" id="D5GE86"/>
    <dbReference type="OMA" id="TWRLCLM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002953">
        <property type="term" value="F:5'-deoxynucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009159">
        <property type="term" value="P:deoxyribonucleoside monophosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3210.10:FF:000011">
        <property type="entry name" value="HD domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039356">
        <property type="entry name" value="YfbR/HDDC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11845">
        <property type="entry name" value="5'-DEOXYNUCLEOTIDASE HDDC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11845:SF13">
        <property type="entry name" value="5'-DEOXYNUCLEOTIDASE HDDC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13023">
        <property type="entry name" value="HD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0170">Cobalt</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD/PDEase" evidence="13">
        <location>
            <begin position="55"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00012964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ82829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="230" mass="26669" checksum="4E0C2FC0EF8312F2" modified="2010-06-15" version="1">MSKTPPQVVGSNTNNFIQNWSLDAASPGSPLAFLHIIERLKTTPREGWRKHGILQGESISDHMYRMSVITMLCPPEHKVDKDRCVKLAIVHDMAEALVGDITPPDKIEKGRRHLPGITRKHRRELESMQYIVNKLLKPISEVIAKDIMDLWMEYETGKTPEAVFVKDVDRFELICQTIEYEKKYEAQKDLKEFLHVRESIKNDFVKKWAEDAMKEREVFWKNAGIESIVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEA2</accession>
    <name>D5GEA2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00001196001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430170">
        <property type="protein sequence ID" value="CAZ82845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838654.1">
        <property type="nucleotide sequence ID" value="XM_002838608.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEA2"/>
    <dbReference type="GeneID" id="9185509"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001196001"/>
    <dbReference type="HOGENOM" id="CLU_336839_0_0_1"/>
    <dbReference type="InParanoid" id="D5GEA2"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="823"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="109"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="264"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="513"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="624"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="61"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="270"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="317"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="343"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="401"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="433"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="458"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="472"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="542"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="554"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ82845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="846" mass="91386" checksum="EA2060506F479783" modified="2010-06-15" version="1">MLHTTSPGSKPDRRASLSVATALSARNISGTSTLGNRANGHSRTVSDSREHLLHVRVHGISSPASPSLSALSSSPPPPRHSPKKPSSLPDSSNIQPGCFDTLSVDSASVASADSVGQTRVRRLCSPENSRRPFVPPPCRQTTNPRSSSQSKDLGGAYTGRLTEQALRDQEAVQHGDLDDTVPKTHLAPYQTITSTPQKPTSPGFGVDTIMASSTREGSGGNGLTSRDRSRSFASTHSDPTRKTEGVAESVVQGTVSKAFGSRLGRLPFGQRSNALGSMGNNEDNGHGEFSQPTVEGSKEKLKPTEGSVDSISGHRLISTSTTSNRGQPFRLITARAEGKARSAQEQSDGNGKQHLGSPSNAPRHGLSFTPAAGTRANSSPKDSKAPRQAVSTLPSPGPDGHLARRDKGHIIKSDVSPAAATQQSQKLSHKRNATTSSIHRTFSGEQGIGKARDERNRSSSAASFRGAATRSKPTSRSVSATSTVELGGSLGVAPTDALVTDANLVTPIPAEALETMRKESRSGRSSSTSSWTDVSEPGADFSSRKPAHDRQRNSRASSTSESTLDTGNYKDPNFEAHAQELSPEIDDADTKPAQTEFEGFAQSFLKRPSEADYEDLALASGAGNFQTSRYTPTPSPSKCRDTRRKTVKVPRGLEDIKLARKSPLEEEDSDVDSLQYLLENDQDFSALFPPVDGEEDDTALLLASLPTYKPPTAQKLKVRPRVEAEAGDQMWEQERAVHEKLMNRLRTLHLEVRSATRGLDVLEGFLDGAGSSGDLSECWDDAAQRKEYMRRLRKEEQKQRLILERRMRQRMALLGTPLPWSKWLVKWGFVAFLVVVVWYLLELAVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFK3</accession>
    <name>D5GFK3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00006956001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430209">
        <property type="protein sequence ID" value="CAZ83296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839105.1">
        <property type="nucleotide sequence ID" value="XM_002839059.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFK3"/>
    <dbReference type="STRING" id="656061.D5GFK3"/>
    <dbReference type="GeneID" id="9188244"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006956001"/>
    <dbReference type="eggNOG" id="ENOG502QRVJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050741_0_0_1"/>
    <dbReference type="InParanoid" id="D5GFK3"/>
    <dbReference type="OMA" id="IAAYQIT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050797">
        <property type="entry name" value="Carb_Metab_Trans_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31668:SF26">
        <property type="entry name" value="GLUCOSE TRANSPORT TRANSCRIPTION REGULATOR RGT1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31668">
        <property type="entry name" value="GLUCOSE TRANSPORT TRANSCRIPTION REGULATOR RGT1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Xylanolytic transcriptional activator regulatory" evidence="6">
        <location>
            <begin position="23"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ83296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="330" mass="36136" checksum="820998FE4D7A0E08" modified="2010-06-15" version="1">MILLGLESDNRAPATMRGLGPPRAVWLGAAVGLAYNLKLHQNRSRGKYANGDSDSDERLGRRAWWVLVILDRWHAISTSTPLFIPDSSVVLVPEDNPLLGSVPFHVARLSCILGHLAEAFTTSDEAVSAALHNPFISKLLRGELERFRESVDGIWGSPNLLHVAYYHVKLLVLRHNPTSEPHQLLGPASRVVTMLASPNTPITPLNHHFAALALVTLVDLAEVQETREDALKGIQQLADALEQRRGIATAEDSFGWDRAIKDLVIRRKMDKTGGSGGGPPEIPPGHGSLQHLAELAVGERSPRDTTDLVRIKQLDTETLQRHGYLGSILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFK6</accession>
    <name>D5GFK6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00006959001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430209">
        <property type="protein sequence ID" value="CAZ83299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839108.1">
        <property type="nucleotide sequence ID" value="XM_002839062.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFK6"/>
    <dbReference type="STRING" id="656061.D5GFK6"/>
    <dbReference type="GeneID" id="9188247"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006959001"/>
    <dbReference type="eggNOG" id="ENOG502S7CA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_517951_0_0_1"/>
    <dbReference type="InParanoid" id="D5GFK6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006878">
        <property type="term" value="P:intracellular copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.430.10:FF:000001">
        <property type="entry name" value="Copper fist DNA-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.430.10">
        <property type="entry name" value="Copper fist DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051763">
        <property type="entry name" value="Copper_Homeo_Regul"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001083">
        <property type="entry name" value="Cu_fist_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036395">
        <property type="entry name" value="Cu_fist_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28088">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR HAA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28088:SF5">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR HAA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00649">
        <property type="entry name" value="Copper-fist"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00617">
        <property type="entry name" value="COPPERFIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM01090">
        <property type="entry name" value="Copper-fist"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00412">
        <property type="entry name" value="Cu_FIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57879">
        <property type="entry name" value="Zinc domain conserved in yeast copper-regulated transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50073">
        <property type="entry name" value="COPPER_FIST_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0186">Copper</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Copper-fist" evidence="9">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="71"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="208"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="373"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="208"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ83299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="526" mass="55433" checksum="22342BC5363FEDDB" modified="2010-06-15" version="1">MACEACIRGHRVSGCNHSDRKLLPIAKKGRPVSQCNHCRQMRKARSAHVKCDCGERLALLKESKAAGKPVPATASACHSSTTPATAAPSEEGDTKDPSSACLCCHGGRCTCALKKEHLDSVPELATPATCASIAPTTTATATVETRKPRLQATHSEGSLTTLHTFANGHHRPTHKHHGSIHGSSPYTIPHVGHTGHTFGEHIGGCTKAPPATVSAPPSAASQKPRRIKSEHSSPDLRSYPALKMANTGITPLELTPQLPQPIKSVSSRVAPGLSVNTNAPYTMHEFRSQELLPSADSECSFQFSAGLYPPQSAGWPPSYNAFDNATFDEQPLDTGVDYSVSQLDIGNYLRFQPSAGLNGSVSGDEIDDFIGPVNGPGGPGGARTVTRSSSSDTSDQAESDFRVSAASSFVALQQASSALLPENSYEDLENLNTAMGPMQPTDPSYGLIIGTVDRALDNETRGLSLYGGYSDGFLSLKNMATTKAAELGGVSSSLPPADDGDYLWMARPITLNSVQDGAPPPEPWIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFL6</accession>
    <name>D5GFL6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00006974001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430209">
        <property type="protein sequence ID" value="CAZ83309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839118.1">
        <property type="nucleotide sequence ID" value="XM_002839072.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFL6"/>
    <dbReference type="FunCoup" id="D5GFL6">
        <property type="interactions" value="112"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GFL6"/>
    <dbReference type="GeneID" id="9188257"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006974001"/>
    <dbReference type="eggNOG" id="ENOG502QRAF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038067_0_0_1"/>
    <dbReference type="InParanoid" id="D5GFL6"/>
    <dbReference type="OMA" id="KVGNAFH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046868">
        <property type="entry name" value="BAR_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046869">
        <property type="entry name" value="SLM1/RGC1-like_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31941">
        <property type="entry name" value="CYTOSKELETAL SIGNALING PROTEIN SLM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31941:SF1">
        <property type="entry name" value="CYTOSKELETAL SIGNALING PROTEIN SLM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20400">
        <property type="entry name" value="BAR_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20399">
        <property type="entry name" value="PH_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SLM1/RGC1-like BAR-like" evidence="4">
        <location>
            <begin position="94"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="SLM1/RGC1-like PH" evidence="3">
        <location>
            <begin position="319"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="427"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="427"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ83309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="491" mass="52805" checksum="D0BFC01B733B6886" modified="2010-06-15" version="1">MATVSPQEDGHPALRHANTIDSMASEEAIPTERGDVAGLLVERLRAWKHAVGYIEAYVSHTEAAHKVMAKEYEKVLKTVSEPLKEGHHFAQDIGGVASFFENVRTNTQGITQSHHETERALKTHVLPILGRLHTEIKDRAKHIHSASEKSSKSVAKARNYTQNHIELLGQHASGFDSMGSGQSTAAGGLAHINIPGTHSHGNGGKLKPDVDPYILRRGVLHRLHKQVIEENNHRQDLLGVQNQMQQFEGHIVQTIQQALGSFYEHVGAQADRQKALYGDIIAAGQRLPLDFEWGGFTTRYADVLIDPSSPARSIESIQFPNQGHQSTKALIEGTLNRKGKVMRSYSAAYYVVTPCNPEISLYLPDCTIGALSKPPNAKFIISGKDAGSTMKALSSKHDYAFKAATHEDAAKWHQIISTCAGLATNESPLQSPISPTTSGPASGAATPPSYSRTGIQELGVTNVGGNGAGPAPARNGVSGVPIVVAGDIKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFS9</accession>
    <name>D5GFS9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007058001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430220">
        <property type="protein sequence ID" value="CAZ83372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFS9"/>
    <dbReference type="STRING" id="656061.D5GFS9"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007058001"/>
    <dbReference type="HOGENOM" id="CLU_1195622_0_0_1"/>
    <dbReference type="InParanoid" id="D5GFS9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="177"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="232" mass="24602" checksum="621B4863CB696376" modified="2010-06-15" version="1">MSFCSSPASQRSSFSSTCSIDCTSPNTAVTTPSSTGATSPKLGQSYTNTQPPSPDAPDISYPYNPTSPIPMNEYPPSSVPPSYAYSCSNQIPSPSPFTYNTTPPPLSASPSNYGYPGYSNYMPESPLQPSPYVHLRSPTPLPSMDSSGSSSMGGGGGFTAASPRKYSGYPPASAFYGPMPNPPAHSASPSSSRYRRKRSETINPEEYKSESWYTADGTKVECSAMHLQCATM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>D5GG38</accession>
    <name>D5GG38_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00001979001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0538 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430237">
        <property type="protein sequence ID" value="CAZ83481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839290.1">
        <property type="nucleotide sequence ID" value="XM_002839244.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GG38"/>
    <dbReference type="FunCoup" id="D5GG38">
        <property type="interactions" value="57"/>
    </dbReference>
    <dbReference type="GeneID" id="9184542"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001979001"/>
    <dbReference type="eggNOG" id="KOG4147">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117792_0_0_1"/>
    <dbReference type="InParanoid" id="D5GG38"/>
    <dbReference type="OMA" id="YRNVKNH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018794">
        <property type="entry name" value="UPF0538"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18444">
        <property type="entry name" value="UPF0538 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18444:SF9">
        <property type="entry name" value="UPF0538 PROTEIN C2ORF76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10209">
        <property type="entry name" value="DUF2340"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="142" mass="15820" checksum="19921CBC55D6D6A9" modified="2010-06-15" version="1">MSSDLLTRPLTSATLTIRVIKNFPYRTAKNLVLHNLDLTTLTSGDLKERVRSEILSSTSGGGGGWKAYKGVVGTLDTLKLYTKAHGTKTNNLIINMEDEGDELIFGDDGRALEGYGCEHETEISFFKLEDYLEFKGNPEEKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>D5GG77</accession>
    <name>D5GG77_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00007247001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430242">
        <property type="protein sequence ID" value="CAZ83520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839329.1">
        <property type="nucleotide sequence ID" value="XM_002839283.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GG77"/>
    <dbReference type="GeneID" id="9184582"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007247001"/>
    <dbReference type="eggNOG" id="ENOG502SAZV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_346888_0_0_1"/>
    <dbReference type="InParanoid" id="D5GG77"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="321"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="506"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="615"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="214"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="23"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="53"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="86"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="102"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="167"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="340"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="509"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="545"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="618"/>
            <end position="630"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ83520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="814" mass="86469" checksum="D793A468B6CBBD0A" modified="2010-06-15" version="1">MSTAEAPTLQNRRLRLRNARTNPSTLPPSSGYYTHRGMLTSPVGRKSSFPEDNGWETEDQGGDGEEEGSNNDNGGEAGRKRRNRVSSSSLLSDGSTLIGGGVDDKAGGGSEDERGSGSNGRPPSPLFSPVGSVRAMTSPIPVRPMSMISTPSPYNYASTAHHRRNRSNGGISSGDSPGSSARYIDYLEHQIADTVSQLQSYTSPSDGTSHAVKMRKLTAEARLLRSELTDWENKFSTRVKEEVNARAAIDKSLRAKIKNLEVKLEEAEYRCKTAEVNLEEARAMISDLRGVEEENRTLEFRIEALSELLADSTRLAQSVGVGKSDCSSVGSGRPGSIALPKRSSSLGSSPVPSSTPSPGTQLRCAAAAADVTATGEKNTPVEGRSWRASRERSTCALEDYGITDPIEAVLYRMSELAATEEEGVEGEIGYSEEYGIELNPNTALVQTSPRPPSAALRSRRMRRFPSGSSAPKTLILPSAAVVTATTITSPTLPTLSDPASVIFAPLSRPPSSSSSSSRCAPPQQAPKAATPQTSLFAELARAQDSSEDSGEDTEAGLDGDDNDATAASISEGLTNPTPLVVKAISKVGESFVSPQGTFFSAKRKAMDILGGVVGNRVSRPHHHHHHRHHLLLSPKPPSSPSSSPSSRRSSRKEFGEKNKKTKAPPTTTTTCAYCHCHQQSPSSTALTAPKAVALARPRSTSAPLVFPQPVEEAVEHVWLWVRFMVALVVALGVAVREGPGVVVAVDGVGSVGGGASAGDGGGFDGVGEEERRLAIRKLQRGAMGGGVVESRGRRRRGLGWGEERVRVWERPGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGC1</accession>
    <name>D5GGC1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00002022001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430262">
        <property type="protein sequence ID" value="CAZ83564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839373.1">
        <property type="nucleotide sequence ID" value="XM_002839327.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGC1"/>
    <dbReference type="STRING" id="656061.D5GGC1"/>
    <dbReference type="GeneID" id="9183167"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002022001"/>
    <dbReference type="eggNOG" id="ENOG502S2UT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1019302_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGC1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.28.10">
        <property type="entry name" value="Homing endonucleases"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Homing_endonucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004860">
        <property type="entry name" value="LAGLIDADG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051289">
        <property type="entry name" value="LAGLIDADG_Endonuclease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36181">
        <property type="entry name" value="INTRON-ENCODED ENDONUCLEASE AI3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36181:SF2">
        <property type="entry name" value="INTRON-ENCODED ENDONUCLEASE AI3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00961">
        <property type="entry name" value="LAGLIDADG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55608">
        <property type="entry name" value="Homing endonucleases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="Homing endonuclease LAGLIDADG" evidence="2">
        <location>
            <begin position="110"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ83564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="321" mass="37806" checksum="2883BEABCE89BFB4" modified="2010-06-15" version="1">MVDLFLSKPWFNYYFFVSGVILANASLDIAFHDTYYSNNKFSLIFESLKQPLAYIIGLLTYYLLFIHYFYLLFSDNTKENNFSNTLININKFLDEKSETDIKNYIEQFFVGLLEGDGTITVDFINNCKKRVRIFIALKNLEDNRFMLNLIVKYIGGRVAIERNNRYVTWYATNRTDLAKIFAILAKYSLLSTRKQCQLDFAKDFINSNLDISELEFKKLRDEKYKNQKTMLDLNDKNFLVKKANNTIKSSQFIIGQNYEKYLLKAILTYFNRENNKIGFTLNKEGVTYYKIHVGGKDFRSLLDSHFNSYPLLGNKNSLYIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGH3</accession>
    <name>D5GGH3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007384001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430264">
        <property type="protein sequence ID" value="CAZ83616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839425.1">
        <property type="nucleotide sequence ID" value="XM_002839379.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGH3"/>
    <dbReference type="GeneID" id="9183214"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007384001"/>
    <dbReference type="HOGENOM" id="CLU_419316_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGH3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="393"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="113"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="188"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="232"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="295"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="341"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="356"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="412"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="654" mass="71069" checksum="C2FBD19FB1A99F92" modified="2010-06-15" version="1">MAHVGLVQCGQEEALLAGQGEPPEMLLIDKRTHDNMRQMALGMLQSSEDGLLHARRTQEVTKSLVEAFDRPPAAKLVLPRLQNPAPSLSSSFVDQPPPPPPHPRPPQADTRRQPGQQSSGQQQQQQQPQQPLNSQSGPASCRPRAPPGGPRNSDDGGGDDGSSGIRRPWGSYSFYTAPTGTGKRNRTTTRGSGSSGTQGVQHQKEHQQEECQQEEEHHHDKDSNNEEDEIVEWTRDDWEWVEKQPGEDSDSTSSISEWDLADWEKLEGIIPGNTPTEKCGQCHTTATASPGDVNRTPTCQSTYSLSERDQSDCNSEAPQGKKTVTPHPLLNPPLQEGQEEPPSKDTSDEHDRRSNKSSPDQYQQHQRNFLPASFSTQPQWAQSQFTNFTHALRRITARVQPQQEATSLPPAGSSTGSSTGSEGEDTHPPGITPRGVATTQATQTTPEQDPTMEFGIASPETSDIPAVGPSEPADARGEWAALLESHPNSAAVEKGRGEVEGNKQKVVEEEYKKVYQELQRILSNACRSWLRTLGASEDSFRALQPKDEVSFGAYRSSFSPLISEDSAQEDNSIGRFLAALDRGQSLQEDIAAEMAAAGGQLSGGLLMEYVDVAREICQPHDDSNREIDELCSRAEIALGSGSSATAAATAESSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGH5</accession>
    <name>D5GGH5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00007386001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430264">
        <property type="protein sequence ID" value="CAZ83618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839427.1">
        <property type="nucleotide sequence ID" value="XM_002839381.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGH5"/>
    <dbReference type="FunCoup" id="D5GGH5">
        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="GeneID" id="9183216"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007386001"/>
    <dbReference type="eggNOG" id="KOG1289">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004495_5_0_1"/>
    <dbReference type="InParanoid" id="D5GGH5"/>
    <dbReference type="OMA" id="LLMAQYT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649">
        <property type="entry name" value="AMINO-ACID PERMEASE BAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649:SF3">
        <property type="entry name" value="POLYAMINE TRANSPORTER TPO5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="28"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="64"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="109"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="227"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="263"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="316"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="369"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="410"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="442"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ83618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="492" mass="53232" checksum="9FA0D416F561F758" modified="2010-06-15" version="1">MIASQNVDNSADTALAKMGYKAELPRNLSMISILGLSFAIMGLFIGLSATMYITLTDGQSVTIIWGWVLVSLISLGIAASLAEICSVLPTAGGVYYWSAMLSTRKYAPIASWITGWLTLVGNWTVTTSINFSGGQLILSAIALWRDDWAPNEWQTILMFWCVMLICALVNILGAKYLDLINKICIYWTAASVVIILVTLLSMADTTRSGEFVFSHFDASASGWPDGWAFFVGLLQAAYTLTGYGMVASMCEEVQQPQREVPRAMVLSVAAAGMTGVVYLVPILFVLPDVKLLLEVPNGQPIGLLFKTVTGSASGGFGLLFLILGILFFAGIGALTAASRCTYAFARGKIREFLTIINGRWSKVDTRYDIPLWGLFLSTMVCCLLGLIYFGRHVRPLTHSLGDAPYSLGKFGVAINILTICWICLAIVLFCMPVSIPVEAPAMNYASVVFAGFTTISAAWYFIRGRKEFTGPPIATDAALVGNTGSRADEEKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGQ7</accession>
    <name>D5GGQ7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00007469001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430275">
        <property type="protein sequence ID" value="CAZ83679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839488.1">
        <property type="nucleotide sequence ID" value="XM_002839442.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGQ7"/>
    <dbReference type="SMR" id="D5GGQ7"/>
    <dbReference type="STRING" id="656061.D5GGQ7"/>
    <dbReference type="GeneID" id="9185601"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007469001"/>
    <dbReference type="eggNOG" id="KOG0118">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013226_1_0_1"/>
    <dbReference type="InParanoid" id="D5GGQ7"/>
    <dbReference type="OMA" id="LENQMDP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12342">
        <property type="entry name" value="RRM_Nab3p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034167">
        <property type="entry name" value="Nab3_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052600">
        <property type="entry name" value="Nuc_rcpt_coact/corep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23295:SF6">
        <property type="entry name" value="NEOSIN, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23295">
        <property type="entry name" value="NUCLEAR RECEPTOR COACTIVATOR 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="303"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="64"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="159"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="358"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="635"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="710"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="90"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="116"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="159"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="170"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="374"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="413"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="477"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="635"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="710"/>
            <end position="720"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ83679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="748" mass="82108" checksum="662BA9AF4C185725" modified="2010-06-15" version="1">MNKQDTQNSENQADGSATNTQTNTDSPVDRIEKRAMLSPISPHPVHVAETVPIPVLNNQMDYALDTPEVHASGPETSNTSVELDYDPETAATTFMEQPKLQQATSSASVSEATNDSSPSSSSSPPSVNKAPAPTEIVQNNRSIPTDVDLQALLTSLSPTVSQHPVSLQGTQTPPAAPTAQTPPSQPRSASLPQPPAVAQVAQSQVHAQPTHPLPAPPATFAGSSNTGAVHTSHPAGYPASLPLPPNFNQHRQAAIPGSPTETDDNEEESRPFTADEEDEYERFLSDERDYVTQGQWDRFPAGSRLFIGNLPTEKVTKRDLFRIFHRHGRLAQVSIKQAYGFVQFLDISSCAAALRHEQGSTVRGRKMHLEVSKPQRNTRPAPSADSQRGSRRSRSPDYGRPVSPRGRGNFRGGRGDYSDRSFRRDTRDRDYEYNRRSSPPRHRVRDDWRPSLRSRSRSPPPPRYRARSRTPPGESLPPRREPKDVPDVQAIVTDELDRNFIWWVEKAFRARSLTYDLLYLHPRMPLDSVIKQQILEGVQAIVFLDSQKQRESSISLQVFNRHGSAEAGYDRYDNLDPAIGAELVIRAKQKAAQPQVSTMPYAYMSQQPFAQLGTLLGSMDPGTLNRLLTGLQAQAQQQQGLQQNHMGQQQPPQQPPSLAPDLAALLAQATTNLGQVTAPQPYGAPTQLSVNTGYGTNAPAGLAALFASQAHTPAQASHPTPTHPTPQQQPQPGQMQNIMETLAKWKQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGV2</accession>
    <name>D5GGV2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00002048001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="FN430276">
        <property type="protein sequence ID" value="CAZ83745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839554.1">
        <property type="nucleotide sequence ID" value="XM_002839508.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGV2"/>
    <dbReference type="STRING" id="656061.D5GGV2"/>
    <dbReference type="GeneID" id="9181721"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002048001"/>
    <dbReference type="eggNOG" id="KOG0747">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_1_14_1"/>
    <dbReference type="InParanoid" id="D5GGV2"/>
    <dbReference type="OMA" id="KLIPLMC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008460">
        <property type="term" value="F:dTDP-glucose 4,6-dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009225">
        <property type="term" value="P:nucleotide-sugar metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05246">
        <property type="entry name" value="dTDP_GD_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000304">
        <property type="entry name" value="UDP-glucose 4,6-dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005888">
        <property type="entry name" value="dTDP_Gluc_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43000">
        <property type="entry name" value="DTDP-D-GLUCOSE 4,6-DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="4">
        <location>
            <begin position="40"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ83745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="389" mass="43739" checksum="4A926D34546B7848" modified="2010-06-15" version="1">MVPNRSVTHRHFQDTGIWRDAPVYKGATKFPPLEDAKNILITGGAGFIACWLVRHLTLTYPEYNIISYDKLDYCASLNNTRCLSTAPNFTFVHGDITSPEEVQNAFRKYNIDTVFHFAAQSHVDLSFGNSYEFTSTNVYGTHVLLECAKNHGSLKRFIHISTDEVYGEVDEDGEDLIEQSILAPTNPYAASKAAAEMLVHSYYKSFKLPVIIVRSNNVYGPHQFPEKVIPKFSCLLHRGEKLLLHGDGKHTRRYLFAGDAADAFDTILHKGLIGQIYNVGSTDEISNLELCALLLKQFGHDPAVDFDSFVEHTIDRPFNDRRYAVDATKLKSLGWVQKTTFAEGLKATVDWYRKFGSEWWGNIDLVLVPFPVVKDSEVVDADNSGSKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGV7</accession>
    <name>D5GGV7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00002053001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430277">
        <property type="protein sequence ID" value="CAZ83750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839559.1">
        <property type="nucleotide sequence ID" value="XM_002839513.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGV7"/>
    <dbReference type="STRING" id="656061.D5GGV7"/>
    <dbReference type="GeneID" id="9181724"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002053001"/>
    <dbReference type="HOGENOM" id="CLU_443582_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGV7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="326"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="342"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="385"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="616" mass="68260" checksum="9B7A26CDDA68143A" modified="2010-06-15" version="1">MFDPPLLILSVARSGVWLNNRADFYSLCLVSKSFYATAIRLLYRKLKISHETFEKSFTPLHAVLSSRQQERIFCLSAAEKHGLARSNGKGPEFGKALSNCGAFVREFEAALLVDDVEEDADIVKEILGSAIDNMSLLESFVWATPSDRALCRTPRISHSLIRKPRLNSLALSNIIETDAPLLRLHPLQSVTFILDSNFVSLAHRLLSSTADYDIIRKILRTSAGTLKSLTLISRPGGQRHYEDENLLFGGGEGDHKSVFKESPEGGEVTHLSRLKSLTLVGRPFTVKRLGVLTQAINFNNLRCLEIVSCPGTELLLSAITLPEARESGMGAESNEVGGSDTDSMSGSNEENGSGSDSDSGMESNKVDWSGLDSMWGSDEEDDPGSGMGAGWASEGGGESATGAEWASAGGNRAFPNLRKLVIRASQYEFGRFLKCFRGLQDLRYELGYDRRTWGLPEGGRRHIPCSLSVDSLLLARISMFHGHTLRKLTILTLDTLLCISERDLRGLARNCRVLVKLCFSARRFHFLFSTNPVKTRTRSNLWTLYLHLESGFSEFQARNCLQRLLLMIPNLKTVGMLTGKHSNEPYIFRVCPGRVLERVFDGDCLQSEFRASKFGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>D5GH11</accession>
    <name>D5GH11_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Elongation factor-like 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00007649001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430297">
        <property type="protein sequence ID" value="CAZ83804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839613.1">
        <property type="nucleotide sequence ID" value="XM_002839567.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GH11"/>
    <dbReference type="FunCoup" id="D5GH11">
        <property type="interactions" value="775"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GH11"/>
    <dbReference type="GeneID" id="9185058"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007649001"/>
    <dbReference type="eggNOG" id="KOG0467">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002794_3_1_1"/>
    <dbReference type="InParanoid" id="D5GH11"/>
    <dbReference type="OMA" id="FARCDIQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01681">
        <property type="entry name" value="aeEF2_snRNP_like_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04096">
        <property type="entry name" value="eEF2_snRNP_like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16268">
        <property type="entry name" value="EF2_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16261">
        <property type="entry name" value="EF2_snRNP_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.240:FF:000006">
        <property type="entry name" value="Elongation factor like GTPase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1430.10:FF:000002">
        <property type="entry name" value="Elongation factor like GTPase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000082">
        <property type="entry name" value="Putative translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.870:FF:000002">
        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1430.10">
        <property type="entry name" value="Yeast translation eEF2 (G' domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056752">
        <property type="entry name" value="EFL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908:SF3">
        <property type="entry name" value="ELONGATION FACTOR-LIKE GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908">
        <property type="entry name" value="TRANSLATION ELONGATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25118">
        <property type="entry name" value="EFL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Tr-type G" evidence="6">
        <location>
            <begin position="1"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00081809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ83804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="897" mass="99235" checksum="56D0326DF0A84E07" modified="2010-06-15" version="1">MESSAISLYFRLMRREAEEAELAPHEYLINLIDSPGHIDFSSEVSTASRLCDGAVVLVDAVEGVCSQTVTVLRQTWVEHIRPILVINKIDRLVTEIKLSPHEAYIHLSKLLGQVNAATGKGSGTDTPTSGYEEKDDEDIYFAPEKDNVIFASAIDGWAFTIRQFAGIYEKKLGIKKSILEKVLWGDYYLDPKTKRVLQQRHLKGRNLKPMFVQLVLDNIWAVYASTVLNKDQEKIEKIVKSLGLKILPREMKSKDTRSLLSTIFSQWLPLSSALLVAVIETLPSPPAAQKQRMPAIVSSAPGSREVSEAVQNAMVGSDKSESAPVMAYVSKMVAGAEDKEHLIGFARLYSGTIKIGQELYVLGPKYSPSRPDQHIHKFTVTDLYYMMGRDLQVLDEIPAGNVFAIGGLEGKLLKSGTLCSMDRGGVNLAGVGLGAAPIVRVAVEPKNPSQLNKVIEGLKLLEQADPCAEYIVQESGEHVLLTAGELHLERCLKDLRERFAKVDIQSSAPIVPYRETIVPATEMSPPKDPNLPRGTVQVVTASKQVSIRIRIRPLPSRVTEFLIENIGSIKKLYSERRGSEEVVVAAENEVSADLEESDGTQKVKVLSLREFKEGLKKSFEGVGQEVWNDVVEKIAAFGPRRIGPNLLIDATGKGLCRKLLHDNALESQRTQTIEDKGATPDIARGLEDRISHAFQLTTAQGPLCHEPIQGIACFVEGAVNSPDDENLEGAAARSRNYEIIASVRDAMRQGFLDWSPRLLMAMYSCDIQASTEVLGKVYGVVTRRRGHIVAEEMKEGTPFFTIKALLPVAESFGFGDDIRKRTSGAASPQLIFTGYEMLFDEDPFWTPFTEDELEDLGELADRENVARKYMDAVRVRKGLPVQKKLVANANKQKTLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHA1</accession>
    <name>D5GHA1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA pseudouridine(55) synthase</fullName>
            <ecNumber evidence="3">5.4.99.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00007716001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a uridine in mRNA = a pseudouridine in mRNA</text>
            <dbReference type="Rhea" id="RHEA:56644"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:14659"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the pseudouridine synthase TruB family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.25" evidence="3"/>
    <dbReference type="EMBL" id="FN430308">
        <property type="protein sequence ID" value="CAZ83853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839662.1">
        <property type="nucleotide sequence ID" value="XM_002839616.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHA1"/>
    <dbReference type="SMR" id="D5GHA1"/>
    <dbReference type="STRING" id="656061.D5GHA1"/>
    <dbReference type="GeneID" id="9182558"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007716001"/>
    <dbReference type="eggNOG" id="KOG2529">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1310905_0_0_1"/>
    <dbReference type="InParanoid" id="D5GHA1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160148">
        <property type="term" value="F:tRNA pseudouridine(55) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990481">
        <property type="term" value="P:mRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002501">
        <property type="entry name" value="PsdUridine_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014780">
        <property type="entry name" value="tRNA_psdUridine_synth_TruB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13767:SF2">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE TRUB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13767">
        <property type="entry name" value="TRNA-PSEUDOURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01509">
        <property type="entry name" value="TruB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Pseudouridine synthase II N-terminal" evidence="7">
        <location>
            <begin position="93"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="71"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ83853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="210" mass="23009" checksum="264DBC0FA30B5743" modified="2010-06-15" version="1">MIPHQTTTGTFTTNLASSLPSQPPILRTAMAPQGLFAVQKPQGLTSAAVIRQLQVIFRNHAWFKPDLTAQQNALDSQSRNQRQRRRRPRQVQVKMGHGGTLDPLATGVLVIGVGTGTKSLSSLISCTKTYETTALFGASTDTYDCTGRILKRALHSHITPQLIESALDAFRGEILQKPPIYSALHHERCSSAQRMRAKGSLCPLISRRGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHJ7</accession>
    <name>D5GHJ7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00007948001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cclA family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430319">
        <property type="protein sequence ID" value="CAZ83990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839799.1">
        <property type="nucleotide sequence ID" value="XM_002839753.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHJ7"/>
    <dbReference type="FunCoup" id="D5GHJ7">
        <property type="interactions" value="86"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GHJ7"/>
    <dbReference type="GeneID" id="9183644"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007948001"/>
    <dbReference type="eggNOG" id="KOG2626">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014420_4_1_1"/>
    <dbReference type="InParanoid" id="D5GHJ7"/>
    <dbReference type="OMA" id="RINFEDM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048189">
        <property type="term" value="C:Lid2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045814">
        <property type="term" value="P:negative regulation of gene expression, epigenetic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12872">
        <property type="entry name" value="SPRY_Ash2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037353">
        <property type="entry name" value="ASH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10598">
        <property type="entry name" value="SET1/ASH2 HISTONE METHYLTRANSFERASE COMPLEX SUBUNIT ASH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10598:SF0">
        <property type="entry name" value="SET1_ASH2 HISTONE METHYLTRANSFERASE COMPLEX SUBUNIT ASH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="4">
        <location>
            <begin position="34"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ83990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="376" mass="42037" checksum="604CE1EC26B3B585" modified="2010-06-15" version="1">MISELPVRYPFLSNLRANVDSPMNRRGFRYTPCEASRMLPTVSYRNTEYAPYAPRINFEDMNAAVHVNRNGSAITTAKGWRMARANVGVREGDWYYECKIVRGIPSGGHVRVGWARREAPLDAPVGFDGYSYGIRDLSGQKVHMSRPKDFLEENFEEGDVVGLRICLPSLDVQRGLTEDPVVRARDLIRDRIPIRFKNQLWFEQFDYQPTKDMEELMNPSPGAKACTPPKTLPNSLIKVYKNGKYVGTPWEDLFAFLPPASRPLATAGGRELDDGTLGYYPAVSVFRGAIVETNFGPDWLAPPDVEDISGMRPLCERYNEQIAEDITYDIVDEVGLLFTKVPGVDDATAAPGVAAEGGENGGLVKEEIKEMVMEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHL1</accession>
    <name>D5GHL1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00007970001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430319">
        <property type="protein sequence ID" value="CAZ84004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839813.1">
        <property type="nucleotide sequence ID" value="XM_002839767.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHL1"/>
    <dbReference type="GeneID" id="9183658"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007970001"/>
    <dbReference type="eggNOG" id="ENOG502QR5Q">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003690_4_2_1"/>
    <dbReference type="InParanoid" id="D5GHL1"/>
    <dbReference type="OMA" id="HEIREMT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000224">
        <property type="term" value="F:peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006516">
        <property type="term" value="P:glycoprotein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2080.10:FF:000001">
        <property type="entry name" value="Alpha-1,2-mannosidase subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.60:FF:000002">
        <property type="entry name" value="Glycosyl hydrolase family 92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.60">
        <property type="entry name" value="alpha-1,2-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1610.10">
        <property type="entry name" value="alpha-1,2-mannosidases domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2080.10">
        <property type="entry name" value="GH92 mannosidase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005887">
        <property type="entry name" value="GH92_a_mannosidase_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041371">
        <property type="entry name" value="GH92_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012939">
        <property type="entry name" value="Glyco_hydro_92"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050883">
        <property type="entry name" value="PNGase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01180">
        <property type="entry name" value="aman2_put"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12143:SF38">
        <property type="entry name" value="ALPHA-1,2-MANNOSIDASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_5G10520)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12143">
        <property type="entry name" value="PEPTIDE N-GLYCANASE PNGASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07971">
        <property type="entry name" value="Glyco_hydro_92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17678">
        <property type="entry name" value="Glyco_hydro_92N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 92 N-terminal" evidence="4">
        <location>
            <begin position="103"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 92" evidence="3">
        <location>
            <begin position="361"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ84004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="858" mass="94150" checksum="FF2B5F73019531DA" modified="2010-06-15" version="1">MGDVSPGGKDPSPPQPEFHASSWSFRDEQTGKLKKRWTAIVVAVGVLLTTTLVTSVVVHSCGLMNKRPRSAHSEDRGSSDSDSDSSQDRDETPKPFVPKLAHLVQPFMGVEGGGNTFPGVCMPYGVVKLGIDMLPPAGVADAYSGYYPRGRVKGFSMMHESGTGGAPKYGVVSQYPVTGALENPLVDHAVARAVPDEAALGWYKASLEGGITVELAASHHAGILKHTFPAMATNASVAGNHVIVDVSYHLQSFRGQGIGQNYVEGSIKVDSNGYEGYGVYNGGWNLGENWKIYFCGKFRTIPVQVRTFSGTGEVLESYGGTSAISGTKRVGAVFSFGASRNITHPLAVESDIGISFISAEKACKFIQSEIPPNQPFGTLVNSTKQTWEEEVLSTVSTAETDDAKLKLLYSAIYGMYIVPSNRTGENPKWESSEPYYDDTFTFWDLFRSHTPLMHISQPGRYEEYIRSTIDIWRHDGYTPDGRSSNFNGRVQGGSNADNVLADAYVKGVRGRINWEDGFKAMVKNAEMTPENNNDQMAPDSSTKEGRGALPDWLERGYITTKYSRSVSRASEYALNDFALSQVAKGLGKTAEYDKYLGRSRNWRNQWDNNSMAFGGEFTGFLSPRDISGNFPTPPQDPLSCGGCYWRDAYYQALPFEYSFGAHHDMETLIKYMGGEEKFVARLEKMFEPGQSPTGSPRFGKTIFNPGNEPSFATPYLFNYAGRQDLTVEKSRYIAKTYYNSGPQGLPGNSDAGAMQSWLVWNMLGLYPITGTTTFLIASPWFSDLTLSLGSPSKTVKITSTGGSETDFFVQSVKLNGNNWDKNWLSWGDLFEKGGTLEFVLGAERTKWDTGERPPSFAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHM2</accession>
    <name>D5GHM2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00008038001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430320">
        <property type="protein sequence ID" value="CAZ84052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839861.1">
        <property type="nucleotide sequence ID" value="XM_002839815.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHM2"/>
    <dbReference type="FunCoup" id="D5GHM2">
        <property type="interactions" value="652"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GHM2"/>
    <dbReference type="GeneID" id="9183706"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008038001"/>
    <dbReference type="eggNOG" id="KOG2066">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001285_0_0_1"/>
    <dbReference type="InParanoid" id="D5GHM2"/>
    <dbReference type="OMA" id="CYIRLQD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030897">
        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034058">
        <property type="term" value="P:endosomal vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057780">
        <property type="entry name" value="Beta-prop_Vps41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000547">
        <property type="entry name" value="Clathrin_H-chain/VPS_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045111">
        <property type="entry name" value="Vps41/Vps8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING VPS41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616:SF1">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 41 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23411">
        <property type="entry name" value="Beta-prop_Vps41"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23556">
        <property type="entry name" value="TPR_Vps41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50236">
        <property type="entry name" value="CHCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Vps41 beta-propeller" evidence="5">
        <location>
            <begin position="86"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="Vps41 beta-propeller" evidence="5">
        <location>
            <begin position="292"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="3">
        <location>
            <begin position="889"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="360"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="365"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ84052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1201" mass="131189" checksum="E5D9C1222E00208E" modified="2010-06-15" version="1">MSSSRPPRGVYRNGDAVSASLVFGERMVIGTHNGNIHILSLPSLNSIRVYHAHSASITSISVSPPPPPPLPAQIRRFAAHQDRNIHVATSAIDGLVCIVPLLPANPGNNSSAAPYSKSEITFKNFKRPIQSVALSPNFRTDRTFISGGLAGNLILSVAPPAGGGGGMAWMGLGGGSGKDTVLHSGEGAISAISWSKESPRFVAWTNEQGIKIMRSHIVPPEIKKEEIAWKRISAIERPESIPEELAAVHKPRLEWIDRRNLSEDDVDGRTLSTADDISPTAVKGKPSIGTDWGQREKLLVGWGGTIWVMDVFAGDGTGEEEKRAGWAEIVHILQTDCTISGLTLYSPSLVLLLAYLKEEKPSSPEPGQASSSAGAGASPSSGSRTRRGRTNALTPELRFIDLNTSEEVSADELLMSRFEGLAVGDYHLGVLPTNPPMTTRPPSASGEGDILGIGTASNYLWAAGMNATQIFSSAASVRSLGSVSRRESFSNASASKASGSLSGLRGMKEKKEDVGWEEYPGKKIYITSPYDVVFATERSPKDHLGWLLEKEKYKDAWELVDAQPDIISSDTASLSEYDEIERIVVDADASDPAYDSDSTISGGRHKVVQMSSAAEKEKRRIGELWLRSLIDVGDWTTAGAVCRKVLGVSGRWEHWVWVFEAAGKIEEITPYIPSTPLSPPLPSVIYEIVLAHYLTHDRRRFQKLLVHDWRPEGSRSLYDPRTIINAVERKLDNREDDVYEGDEDWIILQECLAKLFMAVAEPRNALKFYVILKNADEAFKLIREFRLVDAVKDDILSFIKLRVSDEQMQNASIAELEGGTEEAIGLLVQEAGHGIVGPKMVVRQLDDDKVAGGKLFLFFYLRRLWIGSAGDSSGMGGEGIGIGGGGILAEFGDLMVELFAEYDRPLLMDFLKDSHSYSLEKASAVCERRNYIPELVHLLSKTGQTKRALFLIIDKLADVSQAIAFAKTQDDPDLWNDLLDYSMDKPKFIRGLLENVGTTIDPITLVRRIPTGLEIEGLKAALGKILKEYGVQWSICDGVAKVLRSEVARGMEELRKGQRRGVKFEVAIERELHLSTEVEGPATEVGGTVRDVELEREEIGLGVGGRYACGVCKVDFEPTEKDTLIAFACRHIFHVRCLVIAHGSEADVPEVCGHGAEEEILGVMGRSVGTKVTHAALIRHRVQGGCVLCERKGGEDEFGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>D5GI63</accession>
    <name>D5GI63_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00008304001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430323">
        <property type="protein sequence ID" value="CAZ84206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840015.1">
        <property type="nucleotide sequence ID" value="XM_002839969.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GI63"/>
    <dbReference type="GeneID" id="9181924"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008304001"/>
    <dbReference type="HOGENOM" id="CLU_499647_0_0_1"/>
    <dbReference type="InParanoid" id="D5GI63"/>
    <dbReference type="OMA" id="CENIAWE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="93"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="158"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="519"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="213"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="594"/>
            <end position="613"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ84206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="627" mass="69434" checksum="0C2D80D15E05E7D5" modified="2010-06-15" version="1">MSYSQILSKRFETGQDSPDGYNTRESLIKGSLKGPLKPFSSDDQPRRNRILLARGFSLTFFALVLLMICLWAFSKEHPLSKWEQRSFNTLSILLTASASLGLGSQLGHLGSMLRWPLLARTVHKMRDVDSILGMSPPLGSLRLIKRHIRERRISRTTFIVAAYLITNVVGRLSVAIFGLAYNMTEKTGIEYPILATNWTSALWAGQIFSNGTGDTERDEYESGEGKETAHQTPIGDDISPSLQADLQVSNTTLNLAGKNTVEYSYNLKDFKEARAIPLNHTVHSAANCSIIEVEEGQYWRWDNGNRTGPFNWGERDNADVVSEILTVSNETDVLPYSVTRWTSFLDSLGGSSVYPQIYILCENIAWECWPTLTETAGDNESSQIQPHEKFFHPTDLYRLLTVGSGDYGSLTLGYKDQESELDSDDRGLQGYTYLTGFGGSLTGAEADDGVFYLCNSSLFESHNEAVWDGYRLWMASLIARLPILAIMHANTALPKFARGPHPNQTAGTAYLHTTLEVDWFRVLLIALSITGGQILVILAVLGYCTGVYTRDDSHLATAELLKTVINRFDGGKLMTGEELAASLDDVLEVSVSYGARQGRDGGPPEVDLTSDLDANFPPFPQEKPFTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIB5</accession>
    <name>D5GIB5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008398001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430325">
        <property type="protein sequence ID" value="CAZ84258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIB5"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008398001"/>
    <dbReference type="HOGENOM" id="CLU_2851353_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIB5"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072759" evidence="1">
        <location>
            <begin position="24"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="65" mass="6869" checksum="05750E0F17E3734D" modified="2010-06-15" version="1">MVILSRSLMSCSIGLVFPGSSLGTVPPFPPNRQFHAPSQLLGVENSKDGGDARMLSIKVKNKAEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIK3</accession>
    <name>D5GIK3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00008536001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430327">
        <property type="protein sequence ID" value="CAZ84346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840155.1">
        <property type="nucleotide sequence ID" value="XM_002840109.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIK3"/>
    <dbReference type="FunCoup" id="D5GIK3">
        <property type="interactions" value="449"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GIK3"/>
    <dbReference type="GeneID" id="9183280"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008536001"/>
    <dbReference type="eggNOG" id="KOG1577">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIK3"/>
    <dbReference type="OMA" id="MVNQIFL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000002">
        <property type="entry name" value="2,5-diketo-D-gluconic acid reductase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11732">
        <property type="entry name" value="ALDO/KETO REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="5">
        <location>
            <begin position="18"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="4">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ84346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="296" mass="32763" checksum="12B824F2D464E86B" modified="2010-06-15" version="1">MSVPATFKLNTGAEIPSIGLGTWQSAPGEVQAAVSHALLKGGYKHIDAAYCYGNEDEVGAGLAEAFRGGVKREDIFVTTKLWSTYHSRVELGLDKSLKSLGLDYVDLFLMHWPLPDGSRDLQTDWTYIKTYKEMEKLLSTGKVKAIGVSNFSVPFLKSLLLQNTVVPAVNQIENHPFLPQHDVVDFCKENGTIITAYSPLGSAGGPLLKEESIIKIAEKHNVAVSTVLLNYHINRGNVVLAKSVTPSRIEENNVLLKLDDEDMEALNNIHKEKGTKRFVMPPWPVNFGFPDWKQDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIV7</accession>
    <name>D5GIV7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00008705001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840259.1">
        <property type="nucleotide sequence ID" value="XM_002840213.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIV7"/>
    <dbReference type="FunCoup" id="D5GIV7">
        <property type="interactions" value="78"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GIV7"/>
    <dbReference type="GeneID" id="9185668"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008705001"/>
    <dbReference type="eggNOG" id="ENOG502QPI0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000998_3_0_1"/>
    <dbReference type="InParanoid" id="D5GIV7"/>
    <dbReference type="OMA" id="NDHRYMP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07389">
        <property type="entry name" value="MPP_PhoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.70">
        <property type="entry name" value="PhoD-like phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018946">
        <property type="entry name" value="PhoD-like_MPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038607">
        <property type="entry name" value="PhoD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043904">
        <property type="entry name" value="PhoD_2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46689">
        <property type="entry name" value="MEMBRANE PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46689:SF1">
        <property type="entry name" value="PHOD-LIKE PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19050">
        <property type="entry name" value="PhoD_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PhoD-like phosphatase" evidence="2">
        <location>
            <begin position="319"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="domain" description="PhoD-like phosphatase" evidence="2">
        <location>
            <begin position="581"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="207"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="214"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ84450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="852" mass="95155" checksum="B280B06423BABD19" modified="2010-06-15" version="1">MLGHHSKHKDELDERKKQEGKKKQDERGGYYDTPSQADELHEPQTVYQTPRNPAPISGYDRIGRKGGRHGHHGNPFQHVRRRDGTSGGLGDVRASQALGGEGVFDPRVVRLTLEDVELDDTAPQSNDTAEDSDASTTFSPQLNIKCGPLLRYTGLRRDGTYSENCTAKEERETWRGSIMIVTTDDLSDYSPPPVIRLFARPASEAEIGETNGDASAEAATASTTGERLGARPAEGLPSEKDMPHEDILLRNVVTTRQVNDGEKLGKYKEVEATKLHAERGVTFWRFIVEVELGDEQARIAYRINHGPSIAFWVPARGQTMNIMFHSCNGFSLSVNPDDLCGPDPLWRDVLREHRAKPFHVMLGGGDQIYNDAVSKQTEHFKAWLGIKTPFIKSSHEFTPEMAEELEYFYLERYSMWFSQGLFGVANSQIAMVNIWDDHDIIDGFGSYPHHFNSCPVFTGLGAVAFKYYMLFQHQSIVDEKEEHEPSWVLGCAPGPYIAELSRSCFMFLGRKVAFLGLDCRTERMRDIVLSEESYDLVFERLTKEVVAGETKHLIVLLGVPIAYPRLVWLENIMTSRLMDPLKALGRMGYLGGFLNSFDGGVELLDDLDDHWTAKNHKTERNWFIERLQDFAAEKSVRITILGGDVHLAGIGQFFSSPKLHIPRENDHRYMPNVISSAIVNTPPPNAMADVLNKRNKVHHLNHETDEDMCPIFYTDVDGKPRNNKRLLPRRNYAIIREYSAAIAPLSETAQPPVKRSTFSKIKGATFGSNKSNKPDPLSNVRMAGVDGANTNGPKAVADRLGGDALDVVLRMEVDQKDPAGNTKPYRLLVPALKFEEDAADGGAHDGGGGGWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIX9</accession>
    <name>D5GIX9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008732001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840281.1">
        <property type="nucleotide sequence ID" value="XM_002840235.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIX9"/>
    <dbReference type="STRING" id="656061.D5GIX9"/>
    <dbReference type="GeneID" id="9185690"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008732001"/>
    <dbReference type="eggNOG" id="KOG1818">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_900773_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIX9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd19817">
        <property type="entry name" value="Bbox1_ANCHR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044553">
        <property type="entry name" value="Bbox1_ANCHR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46603">
        <property type="entry name" value="ABSCISSION/NOCUT CHECKPOINT REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46603:SF1">
        <property type="entry name" value="ABSCISSION_NOCUT CHECKPOINT REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22586">
        <property type="entry name" value="ANCHR-like_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="183"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="26"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="309" mass="34319" checksum="FB440067881FDFBC" modified="2010-06-15" version="1">MDDYLARRLAALRNEYETRSFSSSLTPATTIPQTSSSPPKTPKTEVNDELTARFEKMRLTPSSAKSSSVKELKKEYEDILSESKTQEIFNALSSNQEFWDTGEDSHEEMDEVDLLLAEAKAYIPSPPKAPKDVNGAKNALPEDLSVPPDWLHSDTLRPVATTEEEEEADILQRIRDEIDFERANGITTPPEPPSPPGPAEPGEAAPEVDESLFKRFEALGGLELPTVPKAEPGVKPKPHLMAAKPGDDETDTWCCICNDDAEYKCSGCENDIYCASCLYESHTGPDAGYEERRHKWTKYTKPKKRLAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJ17</accession>
    <name>D5GJ17_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Proteasome subunit alpha type</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00008777001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the peptidase T1A family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840319.1">
        <property type="nucleotide sequence ID" value="XM_002840273.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJ17"/>
    <dbReference type="FunCoup" id="D5GJ17">
        <property type="interactions" value="1033"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJ17"/>
    <dbReference type="GeneID" id="9181851"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008777001"/>
    <dbReference type="eggNOG" id="KOG0181">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035750_4_1_1"/>
    <dbReference type="InParanoid" id="D5GJ17"/>
    <dbReference type="OMA" id="ATCIGKD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034515">
        <property type="term" value="C:proteasome storage granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03750">
        <property type="entry name" value="proteasome_alpha_type_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000048">
        <property type="entry name" value="Proteasome subunit alpha type-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 5 6" id="KW-0647">Proteasome</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="7">
        <location>
            <begin position="5"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ84510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="256" mass="27622" checksum="BBEE1991BE18833C" modified="2010-06-15" version="1">MADRYSFSLTTFSPSGKLVQIEYALNAVNQGVTSLGIKATNGVVLATEKKSSTPLMNSSSLEKISCITPNIGMIYSGMGPDYRVLTDKARKVSHSDYKRIYNEYPPTRILVQDVAKVMQEATQSGGVRPFGVSLLVAGWDDKPAGTGKGGPALYQVDPSGSYFPWKATAIGKSATSAKTFLEKRYAEGLELEDAIHIALLTLKETIEGEMSGDTVEIGIVGPPADHLLGYEGVAHAQGPRFRKLSPQEIEDYLTNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJH1</accession>
    <name>D5GJH1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00009007001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430331">
        <property type="protein sequence ID" value="CAZ84664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840473.1">
        <property type="nucleotide sequence ID" value="XM_002840427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJH1"/>
    <dbReference type="GeneID" id="9182665"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009007001"/>
    <dbReference type="HOGENOM" id="CLU_1518953_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJH1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="177" mass="19592" checksum="D85DCD2832E62E00" modified="2010-06-15" version="1">MFLFWIVSNYSSNPQDSIRYLGRCYSPCAENIPGSRHCLLEKKPTADLVLSDLTPCERLQGTVLLPDCGGPPYVNFSAESNPALNRWIFHILLLGCLCPHCYHAHLSPRGMLCLEPTTGIFFKASGEAELLFGAAIYKILYGVPDFVPGSPGGRLRPRATDSADGEMKPFTTSKRFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJN1</accession>
    <name>D5GJN1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">phosphoribosylformylglycinamidine cyclo-ligase</fullName>
            <ecNumber evidence="2">6.3.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00009083001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2.</text>
    </comment>
    <dbReference type="EC" id="6.3.3.1" evidence="2"/>
    <dbReference type="EMBL" id="FN430332">
        <property type="protein sequence ID" value="CAZ84724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840533.1">
        <property type="nucleotide sequence ID" value="XM_002840487.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJN1"/>
    <dbReference type="STRING" id="656061.D5GJN1"/>
    <dbReference type="GeneID" id="9186758"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009083001"/>
    <dbReference type="eggNOG" id="KOG0237">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047116_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJN1"/>
    <dbReference type="OMA" id="MTDYICV"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00129"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004637">
        <property type="term" value="F:phosphoribosylamine-glycine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004641">
        <property type="term" value="F:phosphoribosylformylglycinamidine cyclo-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046084">
        <property type="term" value="P:adenine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02196">
        <property type="entry name" value="PurM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.10:FF:000001">
        <property type="entry name" value="Phosphoribosylformylglycinamidine cyclo-ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.650.10:FF:000007">
        <property type="entry name" value="Trifunctional purine biosynthetic protein adenosine-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00741">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004733">
        <property type="entry name" value="PurM_cligase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00878">
        <property type="entry name" value="purM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10520:SF12">
        <property type="entry name" value="TRIFUNCTIONAL PURINE BIOSYNTHETIC PROTEIN ADENOSINE-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10520">
        <property type="entry name" value="TRIFUNCTIONAL PURINE BIOSYNTHETIC PROTEIN ADENOSINE-3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PurM-like N-terminal" evidence="7">
        <location>
            <begin position="51"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like C-terminal" evidence="8">
        <location>
            <begin position="173"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ84724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="349" mass="37033" checksum="546F70252B3B80C7" modified="2010-06-15" version="1">MTYAGAGVSIETGNALVQRIKNAVRSTRTAGTDAEIGGFGGLYDLKAAGYEDSLLVAGIDGVGTKLKIACALKKHRSVGIDLVAMSVNDLLAQGAAPLFFLDYYSTGELDVEQAAEFIEGVAEGCRQANCALIGGETAEMPGMYALGDYDAAGCAIGAVRRENLLPKLKDMSEGDLLVGVASNGAHSNGYSLIRKIIERHLLDLSAPAPWDSSTTIGEEFLIPTRIYVRPLLKLSSKGLIKGMAHITGGGLTENIPRMLPNHLTAEVDVSTWPLSPLFKWLKQAGALASLEFGRTWNTGLGFVIVVSATEQEQVLEMLKAEGENAYVVGKLVARGSSEGCVLKNVRSWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKB3</accession>
    <name>D5GKB3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00009457001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430337">
        <property type="protein sequence ID" value="CAZ84956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840765.1">
        <property type="nucleotide sequence ID" value="XM_002840719.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKB3"/>
    <dbReference type="FunCoup" id="D5GKB3">
        <property type="interactions" value="258"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GKB3"/>
    <dbReference type="GeneID" id="9181976"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009457001"/>
    <dbReference type="eggNOG" id="KOG1256">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_45_4_1"/>
    <dbReference type="InParanoid" id="D5GKB3"/>
    <dbReference type="OMA" id="KCPIVEH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031956">
        <property type="term" value="F:medium-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031957">
        <property type="term" value="F:very long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015916">
        <property type="term" value="P:fatty-acyl-CoA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015910">
        <property type="term" value="P:long-chain fatty acid import into peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042760">
        <property type="term" value="P:very long-chain fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272:SF33">
        <property type="entry name" value="AMP-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="3">
        <location>
            <begin position="65"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="3">
        <location>
            <begin position="298"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ84956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="667" mass="73994" checksum="44D03F558C3F7900" modified="2010-06-15" version="1">MYTDPVLEYGKTFLPLPPPGSPHAVSVPGSEEEGHTPAFRHWRADPKGELMRSLDPKINTIYDAFEAACQKWPNNKCLGWREQKNATGPWGPYVWMDYETVQKKRADIGAGLSYLHTVHGVTEKQYGIGLWSQNRPEWQIADLAAMSQSLYTVSIYDTLGPETTEYIINHAELHSVVCSLNHVPALLSMRDKVPALKFIVSLDPLEREGEPSGNTKKALLGAWAEEKGIQIYSLAEVESIGRQHPKPCVPPTRDDPITINYTSGTTGNPKGVWLTHGNAVAAVTCSAIGYFHGNILELVDDIKALRPTTFISVPRLYNRFHTSIKTATMEQPGIKGALSRHVVNTKLENMGKTGSNKHMLYDRIWTNKVRAGVGFDRLRASVTGSAPISPTVLSFLRVVFANNFYEGFGMTETYAATVGQLGGDNSAGNCGPPCINVEVRLRDVSDMGYTSKDKPYPRGELLTRGPTVFKTYYKAPEATAGVFDQDGWFATGDICSVDELGRFKVIDRVKNLLKLSQGEYVSPERVENAYLGNMNIFAQAYVYGDPLQPFLVAIFGIDRDHFSSFASKVLRKTVSATDEQAIKAACGNREVIHAVLREMDRVARKTKMAGYERVRNIHLCIDPFTIENDLLTPTLKLKRPPVAKMYKKELSVLYEEALAAQAPKAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKF3</accession>
    <name>D5GKF3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00009512001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430338">
        <property type="protein sequence ID" value="CAZ84996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840805.1">
        <property type="nucleotide sequence ID" value="XM_002840759.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKF3"/>
    <dbReference type="FunCoup" id="D5GKF3">
        <property type="interactions" value="74"/>
    </dbReference>
    <dbReference type="GeneID" id="9182016"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009512001"/>
    <dbReference type="eggNOG" id="ENOG502S81F">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_141719_0_0_1"/>
    <dbReference type="InParanoid" id="D5GKF3"/>
    <dbReference type="OMA" id="KYTVFDR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016340">
        <property type="entry name" value="Ribosomal_mL60"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28271">
        <property type="entry name" value="54S RIBOSOMAL PROTEIN L31, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28271:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09784">
        <property type="entry name" value="L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="99" mass="11859" checksum="A8DD58E2E881144C" modified="2010-06-15" version="1">MLTPPRKSPWRLSSGQKYRHRQRLRRVDRTVDVVYKALAKRGMTAAAVERWKSEMPTEAEMKPRDKYTMFDPKQRSYRKGVHKLPKWTRMSQRVNPPGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKI7</accession>
    <name>D5GKI7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00009580001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRM112 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430339">
        <property type="protein sequence ID" value="CAZ85030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840839.1">
        <property type="nucleotide sequence ID" value="XM_002840793.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKI7"/>
    <dbReference type="FunCoup" id="D5GKI7">
        <property type="interactions" value="804"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GKI7"/>
    <dbReference type="GeneID" id="9184691"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009580001"/>
    <dbReference type="eggNOG" id="KOG1088">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086140_0_0_1"/>
    <dbReference type="InParanoid" id="D5GKI7"/>
    <dbReference type="OMA" id="NMLTSKC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035657">
        <property type="term" value="C:eRF1 methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043528">
        <property type="term" value="C:tRNA (m2G10) methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016435">
        <property type="term" value="F:rRNA (guanine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160102">
        <property type="term" value="F:tRNA (guanine(10)-N2)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000470">
        <property type="term" value="P:maturation of LSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070476">
        <property type="term" value="P:rRNA (guanine-N7)-methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039127">
        <property type="entry name" value="Trm112"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005651">
        <property type="entry name" value="Trm112-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12773:SF0">
        <property type="entry name" value="MULTIFUNCTIONAL METHYLTRANSFERASE SUBUNIT TRM112-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12773">
        <property type="entry name" value="UPF0315 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03966">
        <property type="entry name" value="Trm112p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="140" mass="15509" checksum="99B5AA5E930A1F6C" modified="2010-06-15" version="1">MKILTTNYLTCALRTCKSHPNSFPLHFRDAELQQDSSPFNASFIANILPRLDWSALLTTATELGFTNLPLEKPTVVDERVGMALHRILIETQVVEGKLVCRNCGHEYAIHQGIANFLLPGHLGISPLLLVWGGALAEIYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKM7</accession>
    <name>D5GKM7_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">Pre-mRNA-processing protein 45</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00009646001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in pre-mRNA splicing.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Associated with the spliceosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the SNW family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430340">
        <property type="protein sequence ID" value="CAZ85070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840879.1">
        <property type="nucleotide sequence ID" value="XM_002840833.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKM7"/>
    <dbReference type="FunCoup" id="D5GKM7">
        <property type="interactions" value="920"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GKM7"/>
    <dbReference type="GeneID" id="9184732"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009646001"/>
    <dbReference type="eggNOG" id="KOG2441">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006601_2_0_1"/>
    <dbReference type="InParanoid" id="D5GKM7"/>
    <dbReference type="OMA" id="YGQRRGW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017862">
        <property type="entry name" value="SKI-int_prot_SKIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004015">
        <property type="entry name" value="SKI-int_prot_SKIP_SNW-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12096">
        <property type="entry name" value="NUCLEAR PROTEIN SKIP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02731">
        <property type="entry name" value="SKIP_SNW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="SKI-interacting protein SKIP SNW" evidence="5">
        <location>
            <begin position="188"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="215"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="496"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="231"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="496"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU367140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ85070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="524" mass="57856" checksum="64F5E52D8948C65F" modified="2010-06-15" version="1">MTSLAAGLSKALPAPKYSAEEDVTASRKPTARVLGGGDPDQQGLILKRTGPPPYGRRSAWRPRSADDFGDGGAFPEVPMAQYPLDMGRKRSGTSKALTLQVDNEGKVKYDAIARQGHNDKRIVHASFKDLIPLRQRADVGEISLERPSEEDVAATTEKTRQALARLVSGAVAAQKPKNVKGTNRKDAQYVRYTPSNQLGDTTKRSDKIIKIVERQQDPMEPPKFKHKKIPRGPPSPPPPVMHSPPRKLTAADQEAWTIPPPVSNWKNPKGYTVPLDKRLAADGRGLQDVTINDKFAQFAEALFTADRHAREEIKQRALMQQKLAEKEKSQKEEHLRMLAQKAREAAHTDSRSDDEARKMQMLAREQGRDIGEKIALGLAKPTQSQETMWDSRLFNQSSAFGAGSGFNEDQPYDKPLFAAQEAANSIYRPKVSVDDDDEDTARSEMDRIKRNSRFEVLGKAQQGFRGAADAEARDGPVQFEKDAADPFGVEAFVKAAGEKAGTKHGLQERDSSPGGSKRSRANYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKS5</accession>
    <name>D5GKS5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00009743001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CSN7/EIF3M family. CSN7 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430341">
        <property type="protein sequence ID" value="CAZ85118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840927.1">
        <property type="nucleotide sequence ID" value="XM_002840881.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKS5"/>
    <dbReference type="STRING" id="656061.D5GKS5"/>
    <dbReference type="GeneID" id="9185769"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009743001"/>
    <dbReference type="eggNOG" id="KOG3250">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054426_0_1_1"/>
    <dbReference type="InParanoid" id="D5GKS5"/>
    <dbReference type="OMA" id="GTYKQFR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008180">
        <property type="term" value="C:COP9 signalosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010387">
        <property type="term" value="P:COP9 signalosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045237">
        <property type="entry name" value="COPS7/eIF3m"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041481">
        <property type="entry name" value="CSN7_helixI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15350:SF5">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15350">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 7/DENDRITIC CELL PROTEIN GA17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22061">
        <property type="entry name" value="CSN7_HB_subdom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18392">
        <property type="entry name" value="CSN7a_helixI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0736">Signalosome</keyword>
    <feature type="domain" description="PCI" evidence="8">
        <location>
            <begin position="1"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="222"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="227"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="273"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="289"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="308"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ85118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="323" mass="34240" checksum="F9C1B2F605983AA4" modified="2010-06-15" version="1">MADEQYLTSLQPFILLAKSATGRAAADLVMQATAAPGCFVFSELLEMPNIQALAKTGDGKRYIDLLKIFAYGWYGDYRDNAKNLPPLSAAHLHKLKQLSLITLSSQGPQNLTYTSLQRTLDLPSTRALEDLTISAIYAHLLVAKLDTKAARIEVSSTAGRDVAPEEIPDMIATLKNWCRQCEDVLGDIDEQVRCVQREAIARKRAADEYERIVAMRKEIVKSEEKGSGSGGGMGPGIVSGVPGGGGGGGVAGKGKRVISEGDESAIRGSGGWGEDDEMEIDEGPFDGVTPTSSAGGANTSKRRTRKGPGSGLGGILGSGRSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKU6</accession>
    <name>D5GKU6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00009775001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430341">
        <property type="protein sequence ID" value="CAZ85139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840948.1">
        <property type="nucleotide sequence ID" value="XM_002840902.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKU6"/>
    <dbReference type="STRING" id="656061.D5GKU6"/>
    <dbReference type="GeneID" id="9183375"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009775001"/>
    <dbReference type="eggNOG" id="KOG0729">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_6_3_1"/>
    <dbReference type="InParanoid" id="D5GKU6"/>
    <dbReference type="OMA" id="IKESDTW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048723">
        <property type="entry name" value="OB_PRS7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21236">
        <property type="entry name" value="OB_PRS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="26S proteasome regulatory subunit 7-like OB" evidence="1">
        <location>
            <begin position="89"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF21236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="129" mass="14709" checksum="A01C20B38523B7BC" modified="2010-06-15" version="1">MPSATGKDWEKYKKFADEEEDEKKIIPLSEEDIQVLKTYGAAPYAASLKKLEKDIKDLQTKVNEKIGVKESDTGLAPPHLWDVMGDKQRMSEEQPLQVARCTKIIQSADDPEKAKYVINVKQIAKFGWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKW0</accession>
    <name>D5GKW0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">proline--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Prolyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00009790001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>tRNA(Pro) + L-proline + ATP = L-prolyl-tRNA(Pro) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:14305"/>
            <dbReference type="Rhea" id="RHEA-COMP:9700"/>
            <dbReference type="Rhea" id="RHEA-COMP:9702"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78532"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.15"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.15" evidence="2"/>
    <dbReference type="EMBL" id="FN430341">
        <property type="protein sequence ID" value="CAZ85153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840962.1">
        <property type="nucleotide sequence ID" value="XM_002840916.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKW0"/>
    <dbReference type="STRING" id="656061.D5GKW0"/>
    <dbReference type="GeneID" id="9183389"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009790001"/>
    <dbReference type="eggNOG" id="KOG4163">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001882_4_3_1"/>
    <dbReference type="InParanoid" id="D5GKW0"/>
    <dbReference type="OMA" id="GWKYLNW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004827">
        <property type="term" value="F:proline-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006433">
        <property type="term" value="P:prolyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00862">
        <property type="entry name" value="ProRS_anticodon_zinc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.110.30:FF:000001">
        <property type="entry name" value="Bifunctional glutamate/proline--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.800:FF:000005">
        <property type="entry name" value="bifunctional glutamate/proline--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.30">
        <property type="entry name" value="C-terminal domain of ProRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004499">
        <property type="entry name" value="Pro-tRNA-ligase_IIa_arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016061">
        <property type="entry name" value="Pro-tRNA_ligase_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017449">
        <property type="entry name" value="Pro-tRNA_synth_II"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43382:SF2">
        <property type="entry name" value="BIFUNCTIONAL GLUTAMATE_PROLINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43382">
        <property type="entry name" value="PROLYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09180">
        <property type="entry name" value="ProRS-C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00946">
        <property type="entry name" value="ProRS-C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64586">
        <property type="entry name" value="C-terminal domain of ProRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proline-tRNA ligase class II C-terminal" evidence="11">
        <location>
            <begin position="146"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="169"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="173"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ85153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="236" mass="26209" checksum="00592F7E628B09A8" modified="2010-06-15" version="1">MIHGDNKGLVLPPRVAQYQVVIVPCGLTAKTTEEERKRLLGGVEDIATTLSQAGIRVKADTRETYSPGYKFNDWELKGVPVRLEFGPKDMEKKQVLSARRDTGVKVGIPLSGLAEKVQTLLDTIQKDLYDKAKKAYDDHTIIVKKWEDFTPALNAKNVVLMPHCDQEECEDEVKERSKGRNDDDAPEDAKAPSMGAKSLCIPFDQPKGDDTIIPGTTKCAQCGENAKHYTLFGRSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKW4</accession>
    <name>D5GKW4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">GSTUM_00009795001</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="FN430341">
        <property type="protein sequence ID" value="CAZ85157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840966.1">
        <property type="nucleotide sequence ID" value="XM_002840920.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKW4"/>
    <dbReference type="STRING" id="656061.D5GKW4"/>
    <dbReference type="GeneID" id="9183393"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009795001"/>
    <dbReference type="eggNOG" id="KOG0519">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_3_2_1"/>
    <dbReference type="InParanoid" id="D5GKW4"/>
    <dbReference type="OMA" id="QLIHLMG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071474">
        <property type="term" value="P:cellular hyperosmotic response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06225">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="CDD" id="cd16922">
        <property type="entry name" value="HATPase_EvgS-ArcB-TorS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.130:FF:000002">
        <property type="entry name" value="Two-component osmosensing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1530:FF:000001">
        <property type="entry name" value="Two-component osmosensing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1530:FF:000002">
        <property type="entry name" value="Two-component osmosensing histidine kinase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000015">
        <property type="entry name" value="Two-component osmosensing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1530">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339:SF1">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18947">
        <property type="entry name" value="HAMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158472">
        <property type="entry name" value="HAMP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58104">
        <property type="entry name" value="Methyl-accepting chemotaxis protein (MCP) signaling domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="HAMP" evidence="14">
        <location>
            <begin position="182"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="14">
        <location>
            <begin position="277"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="14">
        <location>
            <begin position="369"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="14">
        <location>
            <begin position="461"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="14">
        <location>
            <begin position="553"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="14">
        <location>
            <begin position="645"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="12">
        <location>
            <begin position="719"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="13">
        <location>
            <begin position="962"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="66"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1114"/>
            <end position="1164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="104"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1147"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="10">
        <location>
            <position position="1014"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAZ85157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1164" mass="127857" checksum="36BB09C57187FA26" modified="2010-06-15" version="1">MQNDEIYQATTAALRALAKGDFQTLGEEPKIYLPGHDTFEKRTLERELLSLTHRIRHLEAKAVAAGRNFPDTPNENGAPSSPFGLGATGHHPSPIPINGALMGSQRNGGSPGSDASDQSSQLEVLHQHVKSQEQEIRENRKIMKNLMEEAQKAKQAPQLPIDEATKVERLQRELKKSQQANEAFSKALREIGEIVTAVAKGDLNKKVTVHAVEMDPEITTFKNTINTMTDQLQTFASEVSRVAREVGTEGRLGGQAKIAGVEGTWMELTNNVNVMASNLTNQVREIADVTTAVANGDLSRKIHVHAQGEILELQQTINSMVDQLRTFAFEVTRVSREVGIDGILGGQAEIREVKGTWRELTDNVNAMAANLTDQVRNIAAVTTAVAEGDLTKKVSALCKGEILQLKETINKMVDRLQNFATEVTKVAREVGTEGKLGGQARVEDVEGTWKSLTENVNSMARNLTTQVRSIASVTTAVAEGNLSRKIDVHAQGEILQLKDTINKMVERLQNFATEVTKVAREVGTDGILGGQARVQDVEGTWKDLTDNVNIMANNLTTQVRSIADVTTAVAQGDLSRKITVHAQGEVLALKSTINKMVDRLEVFSREVKRVARDVGIDGKLGVQAEVNDVDGLWKEITSNVNTMAQNLTTQVRAFGSITAAAADGDFNRFITVEASGEMDALKTKINQMVFNLRDSIQKNTAARETAELANRTKSEFLANMSHEIRTPMNGIIGMTQLTLDTDLTQYQREMLNIVHNLANSLLTIIDDILDISKIEANRMVMEEIPFSLRGTVFNALKTLAVKANERFLDLVYCVDNVVPDHVVGDSFRLRQVILNLVGNAIKFTESGEVKLTIRQHDQSRCSDKEYCFEFSVSDTGIGIQSDKLDLIFDTFCQADGSTTRKFGGTGLGLSISKKLVNLMGGDVWVKSEYGQGSTFYFTCTVRLATTDLALIVPQLKPYRGHHVLFIDKNRENADIPEMLKMLDLKYHHTVQVVENGLQALEAVKNKRYDVILMDVQMPVMGGFEATAKIREYEKDAGLPRTPIVALTAHAMLGDREKCIQAQMDEYLSKPLKQNTLIQVILKCATLGGPLLERGTGILCSIPDDSERVSRVINGATTQLRPRPMLEPRAATANAPIPGASTSSAPLEHADSDTTERSLFRSLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>D5GL93</accession>
    <name>D5GL93_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00010078001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430347">
        <property type="protein sequence ID" value="CAZ85286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841095.1">
        <property type="nucleotide sequence ID" value="XM_002841049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GL93"/>
    <dbReference type="GeneID" id="9182080"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010078001"/>
    <dbReference type="eggNOG" id="ENOG502S18D">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_460187_0_0_1"/>
    <dbReference type="InParanoid" id="D5GL93"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="593" mass="65674" checksum="75DE8624960934E9" modified="2010-06-15" version="1">MRKLFKLDKRSKPRSNTWQKAELDGTEVQISPEKQNRTGSALSGNTAVENSLNFVDPNRGFAKAIQEDEAKSWPHGSGGSSTQNVTTHEGFGSWLSTFQPEAMSSNEHQKNLERLSKLSLIDARGQMPAPEPLISPKVLPDPAPPHGLSHKAPQNCTIVFEGFQESGFKKILGFSPHTARELAIAPWINRSNAYTIRVTSGPSNYSFAHGYCINEHESFIAFFNASIAKSDPIEVIKTAGDELSVDGGKLKISFQRSLRIPETRKTYDLPPKIAKFPLFNVNDFAKRMPGNMAAKGGVCMPLFQREAMWIDFNYPGKGDPGGLSPQYAIKVFFGGVNAISGETWNSSAKKKQDYIVVPPQPWLDGIATGPGVVKQFVAMPLGSGYSVEQQVTGKEDIGGIQMEISPVYNNKFEVSAQCKYNYLGRGTSYGYGSPPSSWSNYSTPKMMACTVGMRIFIKDYRHGDISRDFNSQEIYYDLPVDMAWGDSSMGSSFDSPFVRPAVLRDLLDLSPQEHNLQKPLFVTALNVFSIFVEQDGSLEYPPQVIFVVECSPFATTPQLIQAIEKASSKKHWYTNADYKVQCNGVDMDERLTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLB1</accession>
    <name>D5GLB1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00010116001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430348">
        <property type="protein sequence ID" value="CAZ85304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841113.1">
        <property type="nucleotide sequence ID" value="XM_002841067.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLB1"/>
    <dbReference type="FunCoup" id="D5GLB1">
        <property type="interactions" value="380"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GLB1"/>
    <dbReference type="GeneID" id="9182098"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010116001"/>
    <dbReference type="eggNOG" id="KOG3149">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078004_0_0_1"/>
    <dbReference type="InParanoid" id="D5GLB1"/>
    <dbReference type="OMA" id="GEFDMTI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016514">
        <property type="term" value="C:SWI/SNF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005674">
        <property type="term" value="C:transcription factor TFIIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16905">
        <property type="entry name" value="YEATS_Taf14_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1970">
        <property type="entry name" value="YEATS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027353">
        <property type="entry name" value="NET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038336">
        <property type="entry name" value="NET_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016665">
        <property type="entry name" value="Sas5/TAF14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038704">
        <property type="entry name" value="YEAST_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005033">
        <property type="entry name" value="YEATS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055129">
        <property type="entry name" value="YEATS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23195">
        <property type="entry name" value="YEATS DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17035">
        <property type="entry name" value="BET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03366">
        <property type="entry name" value="YEATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016551">
        <property type="entry name" value="SAS5/TFIID_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51037">
        <property type="entry name" value="YEATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YEATS" evidence="4">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="131"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="149"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ85304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="243" mass="27784" checksum="C84E43141A63E159" modified="2010-06-15" version="1">MTEITRKIKLVTTQSILTDVAPAHEGFPMREWSIRVFIQGEKGEELPANIFDKVTYKLHPTFANPNRVVKKLPFLITEQGWGEFDMEVVLHAIDKGGDHSIRHDLNFQKNKYESVHPVTFNNPRPNLVKALAASGPVPGADTAAPEENGVSKRDKRKNDGEERTKKKSRSERTFDMEKLAEGLQKLQEEHLLQVVQMVHDNKTPETYIKNDVEQGEFHVDLYTLPSHLTQMLWKFVQECGVDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLD1</accession>
    <name>D5GLD1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00010137001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the PP2C family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430348">
        <property type="protein sequence ID" value="CAZ85324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841133.1">
        <property type="nucleotide sequence ID" value="XM_002841087.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLD1"/>
    <dbReference type="FunCoup" id="D5GLD1">
        <property type="interactions" value="244"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GLD1"/>
    <dbReference type="GeneID" id="9186068"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010137001"/>
    <dbReference type="eggNOG" id="KOG0698">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013173_9_2_1"/>
    <dbReference type="InParanoid" id="D5GLD1"/>
    <dbReference type="OMA" id="TFHAVDT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF837">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C-LIKE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 5" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="7">
        <location>
            <begin position="107"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="133"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="250"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="424"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="18"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="59"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="70"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="135"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="258"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="438"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ85324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="447" mass="47926" checksum="0B017F0049D45E9F" modified="2010-06-15" version="1">MFGNNNPLPANTAEASPPAKPTSPPAPPPLNTAAVVTPPPPEKKRRGSVSKASAFIASARNSLSGLGSSNEEKEYEKAIKQDPSTVPVGPHNNSAGDSRPGPKSTFRVGVTEDKNKKCRRTMEDTHSYLYNFLQTSTSNPPGTSSSTATSASGADSSLSNEEPVDDSDNGYFAIFDGHAGTYAADWCGKKLHLVLEETIRKNPNTPIPELLDITFTNCDGQLEKLPVKNSGCTAVCAVLRWEERRLPGALADAPKENGSGNNENAKPATTQQTERQRVLYTANVGDARIVLCRSGKALRLSYDHKGSDENEGKRVANAGGLILNNRVNGVLAVTRALGDSYMKELVTGHPYTTETVLQPEADEFIILACDGLWDVCSDQEAVDLVRQVQDPQEASKMLVDHALGRFSSDNLSCMIVRLDPTGKFNGIKSNDSNEPKEGAPPPLAQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLD7</accession>
    <name>D5GLD7_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Calmodulin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00010144001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430348">
        <property type="protein sequence ID" value="CAZ85330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841139.1">
        <property type="nucleotide sequence ID" value="XM_002841093.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLD7"/>
    <dbReference type="STRING" id="656061.D5GLD7"/>
    <dbReference type="GeneID" id="9186074"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010144001"/>
    <dbReference type="eggNOG" id="KOG0028">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061288_5_1_1"/>
    <dbReference type="InParanoid" id="D5GLD7"/>
    <dbReference type="OMA" id="FTKMMRR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000001">
        <property type="entry name" value="Calmodulin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF0">
        <property type="entry name" value="CALMODULIN LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00020786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ85330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="141" mass="15945" checksum="BBDD6C1A19E8CE11" modified="2010-06-15" version="1">MYVDGDASEDIGEEVIAAKDVRRVLMGMGFESSKEEMNEILEIVDPDDEGWVTYERFLPVASLKLKDRDVHQEAEKAFQIFTAGQPGPITMEHLRRVAERLKEDVTDDQLREMLAVACTKEISRGVDLNDFQAVMKRAGVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLM0</accession>
    <name>D5GLM0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00010266001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430349">
        <property type="protein sequence ID" value="CAZ85413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841222.1">
        <property type="nucleotide sequence ID" value="XM_002841176.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLM0"/>
    <dbReference type="GeneID" id="9182716"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010266001"/>
    <dbReference type="HOGENOM" id="CLU_2639893_0_0_1"/>
    <dbReference type="InParanoid" id="D5GLM0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ85413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="77" mass="9237" checksum="923157CD20BA823B" modified="2010-06-15" version="1">MDVKLKGRCDYYRRIEGRMMMIRKSERYFFFPVFDYFLTLDLVKARVSKRCRALPVLLVRVRGRCLDPLCSEHSDLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLS2</accession>
    <name>D5GLS2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 16">CDP-diacylglycerol--inositol 3-phosphatidyltransferase</fullName>
            <ecNumber evidence="5 16">2.7.8.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">GSTUM_00010381001</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a CDP-1,2-diacyl-sn-glycerol + myo-inositol = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11580"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="2.7.8.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 16 17">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.11" evidence="5 16"/>
    <dbReference type="EMBL" id="FN430351">
        <property type="protein sequence ID" value="CAZ85489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841298.1">
        <property type="nucleotide sequence ID" value="XM_002841252.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLS2"/>
    <dbReference type="FunCoup" id="D5GLS2">
        <property type="interactions" value="622"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GLS2"/>
    <dbReference type="GeneID" id="9186473"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010381001"/>
    <dbReference type="eggNOG" id="KOG3240">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067602_0_0_1"/>
    <dbReference type="InParanoid" id="D5GLS2"/>
    <dbReference type="OMA" id="AQTYSEN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003881">
        <property type="term" value="F:CDP-diacylglycerol-inositol 3-phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006661">
        <property type="term" value="P:phosphatidylinositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1760:FF:000011">
        <property type="entry name" value="Cdp-diacylglycerol-inositol 3-phosphatidyltransferase pis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048254">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014387">
        <property type="entry name" value="CDP_diag_ino_3_P_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15362:SF4">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--INOSITOL 3-PHOSPHATIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15362">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000848">
        <property type="entry name" value="CDP_diag_ino_3_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 16" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="12 16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13 16 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14 16" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="15 16" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 16 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="25"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="159"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="189"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR000848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAZ85489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="231" mass="26209" checksum="AC4AC8F13F245612" modified="2010-06-15" version="1">MPKSNTPTRHSTSPAKSAEPRPENIFLFIPNLIGYSRILLAGASLYYMSYHPHYCTILYSFSCLLDALDGYAARKFSQSTKFGAVLDMVTDRCTTSCLLCFLSSAYPKWAILFQGLISLDLASHYMHMYASLDRGAGSHKKVEKKRSRVLNLYYSNNKILFVFCAANELFFLAMYLLSFPQFSSEIHSWPWIVAMATFPICAAKQWINVVQMVKAAVSLAEGDMEQRRKML</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5GM03</accession>
    <name>D5GM03_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA polymerase epsilon subunit B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">DNA polymerase II subunit 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00010342001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA polymerase epsilon subunit B family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430351">
        <property type="protein sequence ID" value="CAZ85465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841274.1">
        <property type="nucleotide sequence ID" value="XM_002841228.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GM03"/>
    <dbReference type="FunCoup" id="D5GM03">
        <property type="interactions" value="727"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GM03"/>
    <dbReference type="GeneID" id="9182768"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010342001"/>
    <dbReference type="eggNOG" id="KOG3818">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010628_1_0_1"/>
    <dbReference type="InParanoid" id="D5GM03"/>
    <dbReference type="OMA" id="PEDGAWF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008622">
        <property type="term" value="C:epsilon DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042276">
        <property type="term" value="P:error-prone translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007185">
        <property type="entry name" value="DNA_pol_a/d/e_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016266">
        <property type="entry name" value="POLE2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12708:SF0">
        <property type="entry name" value="DNA POLYMERASE EPSILON SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12708">
        <property type="entry name" value="DNA POLYMERASE EPSILON SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04042">
        <property type="entry name" value="DNA_pol_E_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA polymerase alpha/delta/epsilon subunit B" evidence="9">
        <location>
            <begin position="395"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ85465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="663" mass="72675" checksum="5567B4C73F946431" modified="2010-06-15" version="1">MPLDKGKSKLPTFFRPKFPWATPNPSSQQTSDPYEPLPQSTRPQTPRILAVEIPLNVLRPVAFRVFTKKHNLTLKSDALALLCSFVGRRCGAEWRDSGAGEKLLDEVARQWRRNEGANGVLVDGGEGLKSVIRGLEVGGDSVGLSREKSFDITEVPSSALYGGGAGMDDIAAAADAGELEDVEPCAYLKVIDAFSQPKFAYNPLKKQFEKAPKPSLLPPPQTKTQLFRTRYNLLHQRLLRTETFQLPTFSAASSSAAKAVNRLTPISNLLGRSGQSFLLFGMLGVSPSGLLILLDPSGEIVLDLSIATAIPEDGSWFTPGCFCILDGAFEETGKFTVFTVGQPCAERRATSVEVFGHVDFLGNGVTLDMSVSSGGQQGRILRKAEKSLEHVKYLFIGEVELDGKGTLRALRKVFSEYETEPPLVVVLMGNFCSVAMGSSGGSVGYKEYFDQLASLLQDFPTLTSSSTFIFVPGDNDPWASTFSGGASTTLPRKPIPEIFTTRIKRVFTQSRGPNSAIWASNPCRIGYFTQEIVMCRDDIFSRFQRNAINFKKSDHPMEDALSQPEATGDEGEEIDIKTKNTKVSYPHSPSPLDRFYGIIGILLAFTRYLVLQLVLADPRMQPSAITYESCHVMNPGKLVQRRMARWVEYSPSIGKGKVCEAGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMK6</accession>
    <name>D5GMK6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00010779001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430357">
        <property type="protein sequence ID" value="CAZ85749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841558.1">
        <property type="nucleotide sequence ID" value="XM_002841512.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMK6"/>
    <dbReference type="GeneID" id="9182175"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010779001"/>
    <dbReference type="HOGENOM" id="CLU_2869287_0_0_1"/>
    <dbReference type="InParanoid" id="D5GMK6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ85749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="64" mass="7472" checksum="11C7AD856B36D007" modified="2010-06-15" version="1">MLYWIVARSTVAEGELRCLLYPIMIKNFLCLPYVGLGSHNRMPFHIPLFSHLSKYRGKGCKTYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMS1</accession>
    <name>D5GMS1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00010907001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="4">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the arginase family. Agmatinase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430359">
        <property type="protein sequence ID" value="CAZ85814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841623.1">
        <property type="nucleotide sequence ID" value="XM_002841577.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMS1"/>
    <dbReference type="STRING" id="656061.D5GMS1"/>
    <dbReference type="GeneID" id="9185150"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010907001"/>
    <dbReference type="eggNOG" id="KOG2964">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039478_1_1_1"/>
    <dbReference type="InParanoid" id="D5GMS1"/>
    <dbReference type="OMA" id="YELTTIM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008783">
        <property type="term" value="F:agmatinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033389">
        <property type="term" value="P:putrescine biosynthetic process from arginine, via agmatine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11592">
        <property type="entry name" value="Agmatinase_PAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.10:FF:000014">
        <property type="entry name" value="Arginase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.10">
        <property type="entry name" value="Ureohydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005925">
        <property type="entry name" value="Agmatinase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006035">
        <property type="entry name" value="Ureohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020855">
        <property type="entry name" value="Ureohydrolase_Mn_BS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01230">
        <property type="entry name" value="agmatinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358">
        <property type="entry name" value="ARGINASE/AGMATINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358:SF26">
        <property type="entry name" value="GUANIDINO ACID HYDROLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00491">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036979">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00116">
        <property type="entry name" value="ARGINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01053">
        <property type="entry name" value="ARGINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51409">
        <property type="entry name" value="ARGINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0464">Manganese</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072161" evidence="6">
        <location>
            <begin position="20"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR036979-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ85814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="378" mass="41077" checksum="444380729D20F5E2" modified="2010-06-15" version="1">MKSLLSLLLPFLPLALSRAGKEYIEEDILNLRQKWAVEHPFGGISTFAHLQHVRCLVDPHEEFDIAILGAPFDTAVSYRPGARFGPRAIRAASARQSESRGFNVRAGINPYLSDVKILDCGDIPISPFDNELALRQMTEAYKELMSRSTPSGRRPIIVTLGGDHSIALAALRGVNSVYGPVSVVHFDAHLDTWYPTKYPSSWPTLQSAFTHGTMFHLAYNESLLLPTYNIHAGLRTRLSGADMSDYAGDDAQGWARISTDDIDDIGVAGIVKRIVDHVGKSKVYLSIDIDVLDPGIAPGTGTPEAGGWTTRELIRILRGIEGLDVVGVDVVEVAPAYDCAENTALAASQVVFEVLSSLAKKARATVKLEGEGEARDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMS3</accession>
    <name>D5GMS3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00010909001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430359">
        <property type="protein sequence ID" value="CAZ85816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841625.1">
        <property type="nucleotide sequence ID" value="XM_002841579.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMS3"/>
    <dbReference type="FunCoup" id="D5GMS3">
        <property type="interactions" value="31"/>
    </dbReference>
    <dbReference type="GeneID" id="9185152"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010909001"/>
    <dbReference type="eggNOG" id="ENOG502S4D6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096561_0_0_1"/>
    <dbReference type="InParanoid" id="D5GMS3"/>
    <dbReference type="OMA" id="ADWMTKH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040250">
        <property type="entry name" value="Nucleobindin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19237">
        <property type="entry name" value="NUCLEOBINDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19237:SF20">
        <property type="entry name" value="NUCLEOBINDIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072842" evidence="3">
        <location>
            <begin position="23"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="87"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ85816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="201" mass="23299" checksum="9CD94AFB9B2F7027" modified="2010-06-15" version="1">MLKSTLLLLLPLILAHSTTTTAHQSQTADPPKSWAEQHLEQEHHINNFDPGAFFTLHDFDSSGTWTRAEVLRFYGLSQPDSTFSEDTRSHVWRTILDSMDFNHDGEVSREEFQSFCDGGNTLPDFGLGPGHHGDDEYEYEIHHFEQYHNEDTTEDELTHPEDIEHFRKHDQEREAEERQVALEMKPIVEQNIPRKFLVDGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMW5</accession>
    <name>D5GMW5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Inositol-1-monophosphatase</fullName>
            <ecNumber evidence="7">3.1.3.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00010971001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>a myo-inositol phosphate + H2O = myo-inositol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:24056"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:84139"/>
            <dbReference type="EC" id="3.1.3.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 6 7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="7">Polyol metabolism; myo-inositol biosynthesis; myo-inositol from D-glucose 6-phosphate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the inositol monophosphatase superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.25" evidence="7"/>
    <dbReference type="EMBL" id="FN430360">
        <property type="protein sequence ID" value="CAZ85858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841667.1">
        <property type="nucleotide sequence ID" value="XM_002841621.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMW5"/>
    <dbReference type="FunCoup" id="D5GMW5">
        <property type="interactions" value="390"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GMW5"/>
    <dbReference type="GeneID" id="9183426"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010971001"/>
    <dbReference type="eggNOG" id="KOG2951">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044118_1_2_1"/>
    <dbReference type="InParanoid" id="D5GMW5"/>
    <dbReference type="OMA" id="ERGLHPW"/>
    <dbReference type="UniPathway" id="UPA00823">
        <property type="reaction ID" value="UER00788"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008934">
        <property type="term" value="F:inositol monophosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006021">
        <property type="term" value="P:inositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01639">
        <property type="entry name" value="IMPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.540.10:FF:000004">
        <property type="entry name" value="Inositol-1-monophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.10">
        <property type="entry name" value="Fructose-1,6-Bisphosphatase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033942">
        <property type="entry name" value="IMPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020550">
        <property type="entry name" value="Inositol_monophosphatase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20854">
        <property type="entry name" value="INOSITOL MONOPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20854:SF4">
        <property type="entry name" value="INOSITOL-1-MONOPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00377">
        <property type="entry name" value="IMPHPHTASES"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56655">
        <property type="entry name" value="Carbohydrate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00630">
        <property type="entry name" value="IMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 6 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR600760-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ85858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="283" mass="30604" checksum="58440E643BB606B3" modified="2010-06-15" version="1">MVDPINHKEVHSLLVEVALAAGDMILAAQPHIATVGTKQNSADLVTETDKAVEQYVITTLKGKYPELDFLGEETYTIGKKLTNKPTFICDPIDGTVNFVHTLPNVCISLALAIHKKPVVGVVYNPYTTHLYSAIKGQGSFLTMPVLLQVQAPSQPQPSPTRGTGINWNIKYETFAKLAGGREQGGKMVHSLRSMGSAALNFCAVAAGQIDVYWEGGCWAWDVAAGWLILTEAGGIVVDANPGNWSPTIDGRRYMAVRAAPAGQREIVEEFWSIIPEGGYDYSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>D5GN19</accession>
    <name>D5GN19_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00011052001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430362">
        <property type="protein sequence ID" value="CAZ85912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841721.1">
        <property type="nucleotide sequence ID" value="XM_002841675.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GN19"/>
    <dbReference type="GeneID" id="9183480"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011052001"/>
    <dbReference type="HOGENOM" id="CLU_1972080_0_0_1"/>
    <dbReference type="InParanoid" id="D5GN19"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="12"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="116"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="127" mass="14267" checksum="B1022D442512F2E0" modified="2010-06-15" version="1">MGIQLIPREKSLLFRKKEEKEKKKGYPGQSTSPPSYPSLSRSKQAANKRIAELNLSGRKKATIGSFVNVEPRNRLIDKAPEVQCNAVQIHVLSGQRAGEVERERGDDRRHTRSPAFLPSFLPSFLPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>D5GN70</accession>
    <name>D5GN70_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00011112001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430362">
        <property type="protein sequence ID" value="CAZ85963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841772.1">
        <property type="nucleotide sequence ID" value="XM_002841726.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GN70"/>
    <dbReference type="STRING" id="656061.D5GN70"/>
    <dbReference type="GeneID" id="9185889"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011112001"/>
    <dbReference type="eggNOG" id="KOG0029">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_829470_0_0_1"/>
    <dbReference type="InParanoid" id="D5GN70"/>
    <dbReference type="OMA" id="YVTFPRA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050281">
        <property type="entry name" value="Flavin_monoamine_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742:SF414">
        <property type="entry name" value="CONTAINING AMINE OXIDASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G12150)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742">
        <property type="entry name" value="FLAVIN MONOAMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00419">
        <property type="entry name" value="ADXRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="1">
        <location>
            <begin position="42"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="335" mass="37025" checksum="16ABEE610D37667A" modified="2010-06-15" version="1">MSPMAPNTDGTVDILPPISTELPKELSLVGKTPTIAVIGGGMSGLRAAEVLLQKGYRVVVYEARDRLGGRISTSNHLGEAVDLGPNWIHGTDNNPVLSLAESSGSKLHHFSEQCPTYGFDGKLLDQAEADELSELMWKVIERAVEYSKHCHPAIPVEKSFYDYCVEKAEELFGTGAAEDEKRKKELWLSLAEMWGTFIGSPVERQSLKYFFLEEPLEGANIFVASTYETMIDTMSKPSLDAGIVRLNTPIDEIITPAPGDRNCVMLRSSQKPEENCEVDAVIVTIPLGCLKREMINFQPKLPKRMLEGIDSLSYGTLEKARFSHLKAGYSKLKIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNL8</accession>
    <name>D5GNL8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00011357001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430366">
        <property type="protein sequence ID" value="CAZ86111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841920.1">
        <property type="nucleotide sequence ID" value="XM_002841874.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNL8"/>
    <dbReference type="FunCoup" id="D5GNL8">
        <property type="interactions" value="638"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GNL8"/>
    <dbReference type="GeneID" id="9186187"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011357001"/>
    <dbReference type="eggNOG" id="KOG2830">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041824_2_0_1"/>
    <dbReference type="InParanoid" id="D5GNL8"/>
    <dbReference type="OMA" id="EYELCEA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051721">
        <property type="term" value="F:protein phosphatase 2A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045943">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035303">
        <property type="term" value="P:regulation of dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903432">
        <property type="term" value="P:regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.540">
        <property type="entry name" value="TAP42-like family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038511">
        <property type="entry name" value="TAP42/TAP46-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007304">
        <property type="entry name" value="TAP46-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10933">
        <property type="entry name" value="IMMUNOGLOBULIN-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10933:SF9">
        <property type="entry name" value="IMMUNOGLOBULIN-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04177">
        <property type="entry name" value="TAP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="216"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="296"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="216"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="296"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ86111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="350" mass="40047" checksum="9D432A1EC0FA3AF2" modified="2010-06-15" version="1">MSENQESLRSLYDSGERLRKEIEESYDYNSPAYQQKLQTAIRTYQECKKLVDQISLFSTNESLEDVVSSEIRYMLIDYHFADLTSKQQSPDRASILKLSKFLYMRFLNLCDAYDLLSNYDRSVLHPADPEAPKASPAPIDAAARRNEKIAKFKLEKELKTKIDALAQNPHLLDEDELRKLQTALMSLAIMKSIQQLEMIELELDILSKAPKIEENPVTRLTKDERSRDRGSRDGYNDKLDNIPSTIKGGPLLSQDGKPLRPFTLLDSRERLKQGVFKSGHNLPTMTIDEYLEEERKRGGIIEGGGEKSGIRSEPDEDNHEKADEETMKAREWDEFKEANPRGSGNTINRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNR8</accession>
    <name>D5GNR8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Casein kinase II subunit beta</fullName>
            <shortName evidence="4">CK II beta</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00011446001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 4">Regulatory subunit of casein kinase II/CK2. As part of the kinase complex regulates the basal catalytic activity of the alpha subunit a constitutively active serine/threonine-protein kinase that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Tetramer composed of two alpha chains, one beta chain and one beta' chain.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the casein kinase 2 subunit beta family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430367">
        <property type="protein sequence ID" value="CAZ86165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841974.1">
        <property type="nucleotide sequence ID" value="XM_002841928.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNR8"/>
    <dbReference type="FunCoup" id="D5GNR8">
        <property type="interactions" value="921"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GNR8"/>
    <dbReference type="GeneID" id="9182800"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011446001"/>
    <dbReference type="eggNOG" id="KOG3092">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_720006_0_0_1"/>
    <dbReference type="InParanoid" id="D5GNR8"/>
    <dbReference type="OMA" id="RHIFACI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005956">
        <property type="term" value="C:protein kinase CK2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034456">
        <property type="term" value="C:UTP-C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019887">
        <property type="term" value="F:protein kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006359">
        <property type="term" value="P:regulation of transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1820.10:FF:000003">
        <property type="entry name" value="Casein kinase II subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.20:FF:000001">
        <property type="entry name" value="Casein kinase II subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1820.10">
        <property type="entry name" value="protein kinase ck2 holoenzyme, chain C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016149">
        <property type="entry name" value="Casein_kin_II_reg-sub_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035991">
        <property type="entry name" value="Casein_kinase_II_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000704">
        <property type="entry name" value="Casein_kinase_II_reg-sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11740">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11740:SF39">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01214">
        <property type="entry name" value="CK_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00472">
        <property type="entry name" value="CASNKINASEII"/>
    </dbReference>
    <dbReference type="SMART" id="SM01085">
        <property type="entry name" value="CK_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57798">
        <property type="entry name" value="Casein kinase II beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01101">
        <property type="entry name" value="CK2_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="347"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="348"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="371"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00045899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00062110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ86165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="384" mass="43949" checksum="FA8EC3BDE698C00B" modified="2010-06-15" version="1">MDLTGAARVRSAGTARSLRLHQPNQPCQSRADQPGLMNQPHSPGSTYAEIYTNDLNSSIQIRYISFHQQKTTRPLTLFASPLSFITIVSNQPTSLNFVLVRLTEPVLYIVKPCGCFYEIQRSRTGASLLGSNRSLKAKETIIMEDFNSETDSDYASYWRDWFISTRGHEYFCEIDEEYLTDRFNLTGLQSEVQYYQYALDLVTDQFDFECEDDMREQIEKSARHLYGLVHARYIITTRGLAKMLEKYKKCDFGRCPRVLCRSHPLLPLGLSDLPHQKPVKLYCAKCEDVYSPKSSRHGAIDGAYFGASFPHILFQVYPGLIPPKSNERYTPRCFGFKTHAQAALSRWQEGEREAQDARLEKAGVEVRRVDGEEDEDEDEEVAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5GP13</accession>
    <name>D5GP13_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00011619001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430371">
        <property type="protein sequence ID" value="CAZ86256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842065.1">
        <property type="nucleotide sequence ID" value="XM_002842019.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GP13"/>
    <dbReference type="FunCoup" id="D5GP13">
        <property type="interactions" value="216"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GP13"/>
    <dbReference type="GeneID" id="9183549"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011619001"/>
    <dbReference type="eggNOG" id="KOG2187">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014689_3_1_1"/>
    <dbReference type="InParanoid" id="D5GP13"/>
    <dbReference type="OMA" id="GGCKWQH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051908">
        <property type="term" value="F:double-stranded DNA 5'-3' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000014">
        <property type="term" value="F:single-stranded DNA endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030697">
        <property type="term" value="F:tRNA (uracil(54)-C5)-methyltransferase activity, S-adenosyl methionine-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000201">
        <property type="entry name" value="TRM2p tRNA methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030390">
        <property type="entry name" value="MeTrfase_TrmA_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030391">
        <property type="entry name" value="MeTrfase_TrmA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025795">
        <property type="entry name" value="tRNA_(uracil-5-)_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010280">
        <property type="entry name" value="U5_MeTrfase_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11061">
        <property type="entry name" value="RNA M5U METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11061:SF30">
        <property type="entry name" value="TRNA (URACIL(54)-C(5))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05958">
        <property type="entry name" value="tRNA_U5-meth_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51687">
        <property type="entry name" value="SAM_MT_RNA_M5U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51622">
        <property type="entry name" value="SAM_MT_RNA_M5U_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01230">
        <property type="entry name" value="TRMA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01231">
        <property type="entry name" value="TRMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TRAM" evidence="7">
        <location>
            <begin position="75"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="35"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="461"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="461"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="366"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="387"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="434"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ86256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="522" mass="57481" checksum="374D2F07FAF87D8F" modified="2010-06-15" version="1">MSAPLPPPPGPSNTRPQFRQHDGGSPRNQKRRRLERAPKPVKEGGAEEVLQTDISSLMSALGLTRPEKPFPEQGLPPISSEIELTIKMLSSTGDGLAEHSGRIYVVPFSIPGDIITAKVIRHTTTHSITDFLSVKSPSQDRDDSLIGCKYFNTCSGCQFQMLPYAKQLEHKRNVILKAYDNFSNLDPKLLPGIGDTVGSPLQYSYRTKLTPHFDGPRRGGFKAGAPTPDIGFMIKGRRQVLDIEDCPIGTDILRKGIKTARKYVEENLHTYKRGATILLRESTTRRPREEGEEGNTKWVEEKECITDNNAQTTEYIGDYKFVNPAGAFFQNNNSILPALHYPVLTTSPDGTRTTISTSPKYLVDAYCGSGLFTILCGKNLTSVIGVDIAADSIAYASSNAAANNIENARFIAGNAETIFESIDFPSTETSVIIDPPRKGCDKPFLDQLLEFKPKRVVYVSCNVHTQARDLGYILNGKGSGYRVDEIRGFDFFPQTHHVEGVAILTWIGEEGIASLPEDVNNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5GP45</accession>
    <name>D5GP45_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NAD(+) diphosphatase</fullName>
            <ecNumber evidence="4">3.6.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00011698001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 5'-end NAD(+)-phospho-ribonucleoside in mRNA + H2O = a 5'-end phospho-adenosine-phospho-ribonucleoside in mRNA + beta-nicotinamide D-ribonucleotide + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60876"/>
            <dbReference type="Rhea" id="RHEA-COMP:15698"/>
            <dbReference type="Rhea" id="RHEA-COMP:15719"/>
            <dbReference type="ChEBI" id="CHEBI:14649"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:144029"/>
            <dbReference type="ChEBI" id="CHEBI:144051"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:60877"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Nudix hydrolase family. NudC subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.22" evidence="4"/>
    <dbReference type="EMBL" id="FN430373">
        <property type="protein sequence ID" value="CAZ86310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842119.1">
        <property type="nucleotide sequence ID" value="XM_002842073.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GP45"/>
    <dbReference type="SMR" id="D5GP45"/>
    <dbReference type="FunCoup" id="D5GP45">
        <property type="interactions" value="89"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GP45"/>
    <dbReference type="GeneID" id="9186550"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011698001"/>
    <dbReference type="eggNOG" id="KOG3084">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037162_0_2_1"/>
    <dbReference type="InParanoid" id="D5GP45"/>
    <dbReference type="OMA" id="CNTRTTL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035529">
        <property type="term" value="F:NADH pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019677">
        <property type="term" value="P:NAD+ catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006742">
        <property type="term" value="P:NADP+ catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03429">
        <property type="entry name" value="NUDIX_NADH_pyrophosphatase_Nudt13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000042">
        <property type="entry name" value="Probable NADH pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050241">
        <property type="entry name" value="NAD-cap_RNA_hydrolase_NudC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015375">
        <property type="entry name" value="NADH_PPase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049734">
        <property type="entry name" value="NudC-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015376">
        <property type="entry name" value="Znr_NADH_PPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42904:SF6">
        <property type="entry name" value="NAD-CAPPED RNA HYDROLASE NUDT12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42904">
        <property type="entry name" value="NUDIX HYDROLASE, NUDC SUBFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09296">
        <property type="entry name" value="NUDIX-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09297">
        <property type="entry name" value="Zn_ribbon_NUD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="10">
        <location>
            <begin position="256"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ86310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="415" mass="45624" checksum="93A0BA57F84C5227" modified="2010-06-15" version="1">MTQPYTATTESHKTSMLSRKFGQEIINYFSGSPLNRVSFLRSSTAFLRAALPRSKFLLMRDLEPLAASKTTLAWVGYETVRELVGNPFDTEEEEFLRGYDSRVEIPTLVFLGMNEEGTGGGGGGESFEFEGEGGKYVGGPCFALDVTEKGSLKEECEKVQEEAVKGAEGREFRKVRLDLSLVPSDAAILAQARSLLDWNNRNQFCSACGARTVSVHAGTKRICPPSDKAFQKDPEGPPFERPPCISRKGIHNIAFPRTDPTVIMAIINSTGDKVLLGRQRRWPKDFYSTLAGFCEPAESVEDAVRRETWEESGVRVGRVVIHSTQPWPYPANLMMGAIGEALPGGEEIVLKHDPELEDAQWVEVGRVRNALQESTGTPDVEVEGAIEGQLRLPPSTAIAHQLLLAVVNGFHLAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5G402</accession>
    <name>D5G402_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ornithine carbamoyltransferase, mitochondrial</fullName>
            <ecNumber evidence="4">2.1.3.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Ornithine transcarbamylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00003888001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>carbamoyl phosphate + L-ornithine = L-citrulline + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19513"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="EC" id="2.1.3.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the aspartate/ornithine carbamoyltransferase superfamily. OTCase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.3" evidence="4"/>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835124.1">
        <property type="nucleotide sequence ID" value="XM_002835078.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G402"/>
    <dbReference type="FunCoup" id="D5G402">
        <property type="interactions" value="588"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G402"/>
    <dbReference type="GeneID" id="9182372"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003888001"/>
    <dbReference type="eggNOG" id="KOG1504">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043846_3_0_1"/>
    <dbReference type="InParanoid" id="D5G402"/>
    <dbReference type="OMA" id="DGNNVCN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903269">
        <property type="term" value="C:ornithine carbamoyltransferase inhibitor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004585">
        <property type="term" value="F:ornithine carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019240">
        <property type="term" value="P:citrulline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:L-arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1370:FF:000017">
        <property type="entry name" value="Ornithine carbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1370:FF:000009">
        <property type="entry name" value="Ornithine carbamoyltransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006130">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002292">
        <property type="entry name" value="Orn/put_carbamltrans"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00658">
        <property type="entry name" value="orni_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001986">
        <property type="entry name" value="PRK00779.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45753">
        <property type="entry name" value="ORNITHINE CARBAMOYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45753:SF3">
        <property type="entry name" value="ORNITHINE TRANSCARBAMYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00102">
        <property type="entry name" value="OTCASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00097">
        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aspartate/ornithine carbamoyltransferase carbamoyl-P binding" evidence="13">
        <location>
            <begin position="45"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="Aspartate/ornithine carbamoyltransferase Asp/Orn-binding" evidence="12">
        <location>
            <begin position="195"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ79245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="360" mass="38959" checksum="F2F659AD13202A71" modified="2010-06-15" version="1">MTFPIFRRAQLPHVFRNSSRSILTARAGNPAGAGSVPQTSPFALRHLLSIDDLTSSEFVKLVGQARRAKQEFKAGQTSPSLKDTLTGRTVAMMFSKRSTRTRVSTEAAVVYLGGHPMFLGKDDIQLGVNESLPDTSRVISSMVSCMVARVGSHSDVVELSKTSTVPVINALSDAFHPLQAVADILTITEIFPEPKGIKVAWVGDANNVLCDLAIACAKCGINVSIATPSAYPVPPDMLQLARNAAHLSGSSIVTTHEPEAAVKGAHIIVTDTWVSMGQESEKVIRLKQFAGFQVSLDLAERGGANKDWRFMHCLPRKPEEVTDEVFFSPRSLVFPEAENRLYAAIAALEAFVIKQGNFGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5G465</accession>
    <name>D5G465_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00003968001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the palA/RIM20 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835187.1">
        <property type="nucleotide sequence ID" value="XM_002835141.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G465"/>
    <dbReference type="FunCoup" id="D5G465">
        <property type="interactions" value="1151"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G465"/>
    <dbReference type="GeneID" id="9182435"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003968001"/>
    <dbReference type="eggNOG" id="KOG2220">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007181_2_1_1"/>
    <dbReference type="InParanoid" id="D5G465"/>
    <dbReference type="OMA" id="VSHAEEM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09241">
        <property type="entry name" value="BRO1_ScRim20-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.560">
        <property type="entry name" value="alix/aip1 in complex with the ypdl late domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.50">
        <property type="entry name" value="alix/aip1 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.280">
        <property type="entry name" value="alix/aip1 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025304">
        <property type="entry name" value="ALIX_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004328">
        <property type="entry name" value="BRO1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038499">
        <property type="entry name" value="BRO1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23030">
        <property type="entry name" value="PCD6 INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23030:SF39">
        <property type="entry name" value="PROGRAMMED CELL DEATH 6-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13949">
        <property type="entry name" value="ALIX_LYPXL_bnd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03097">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01041">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51180">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRO1" evidence="4">
        <location>
            <begin position="5"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="485"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="764"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="683"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="489"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="771"/>
            <end position="787"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ79308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="843" mass="93763" checksum="0220F3A895F4F1E0" modified="2010-06-15" version="1">MTSSNILHVPFRKSAPLQLSTAIKQYISTKYDQHPDMFTTDLAAIDKLRSDAINVQEAHVSGVMKISVYAAQLQWISGKFPIDIGADFTWYPALGYNTQRPVTQNNLQFERANVLYNLGSLYSQLASSSPRTTAQGLKTACNYFCAAAGVFTYLKKEVLTELRSTPPEDMDAGTLESLEMLMLAQAQECFWLKAAVDGHKDSLVAKLAAMVSDFYDLAGEFGMKSDSISSEWMHHLSAKHHHFAAAAQYRAACDCLERSLYGEEVARLKDSLDAVNEALRGARYLNKVVLGDLQGLKEKVTETLKGAEKDNDLLYFQAVPPASELPKIPRAPMVEPKIPREVSEAANLLSEGGAYGPPLFSKLVPFAVHQAASIYAERRDRLVNNVLADKLESLTNKLHELLRSLNLPGSLQALEKPLGLPPGLMSHAEEIKQQGGVATLLKSMDDIAKLKANDNAMYQEVLSPRLSLTRSALTRTQAVEMLDTEAAEDERARGKHGTDQWNREPSSQAASKLMASVQEYAGILKSADNSDGLVKVKLNECEKSLRLLGGDIHSLQDFVPNSSRASLTPKMEREVAKLRQCLNEISRLESRRRRKIESLREKAKADDITSAILAEAARLERESPLQKLEPVHFEPLFESRLTAKYDGENTLLKEEAEVQKDIISRTQEANSSFAACRKVDSSLKEREQALQTLENAHSKYREILDNLNAGRKFYNDLAKLLARFRDECKDFVYQRRIEAGQFEVEITNAMQAVRLSQTPILQPTTQHYHHQSPPQQKLQQQQQHIPLQSPPLPGVGQQQRRVPPGTPLPAPVPTRAAIPPLSPGLWKGDATTAIRFAGEPSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4B5</accession>
    <name>D5G4B5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00004034001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TCP11 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835237.1">
        <property type="nucleotide sequence ID" value="XM_002835191.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4B5"/>
    <dbReference type="GeneID" id="9184983"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004034001"/>
    <dbReference type="eggNOG" id="KOG1981">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005050_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4B5"/>
    <dbReference type="OMA" id="FHAWKTH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010737">
        <property type="term" value="P:protein kinase A signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008862">
        <property type="entry name" value="Tcp11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12832:SF18">
        <property type="entry name" value="IQ CALMODULIN-BINDING MOTIF DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_1G08920)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12832">
        <property type="entry name" value="TESTIS-SPECIFIC PROTEIN PBS13 T-COMPLEX 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05794">
        <property type="entry name" value="Tcp11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="148"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="444"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="32"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="148"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="444"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="468"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ79358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="944" mass="105808" checksum="1B8D0C38961155EE" modified="2010-06-15" version="1">MLHHDENSASSPGWNPPAHLEARFSRKRRRPGTSSPCNHPLTNSNLATTRRQTLLETRKSLLRQRAAHVEKVRRARANSHEAVSERLLALQQSLSQAHNSRNAILAKIAGSCASEVAKAKQVAQEIRARREEEAKALKSGVEERMLEAERRRKEVLRERRGKRRERGSSSSVKVDGSSEEEGDDAVAGSLQKIRLGASKPTEMLREEAAIHIQRLYRNYRNREVVGRFLELGLTIESVRDTSFEAISIKFQEEKVRKATTRLLKLCGLLEHLEEGRVEIEKACRTFLSAFLILGHPGEVLSDDGESEKVIPTDNPSPKMHANLNRFKQALITKSKDLLIAFESFLSSPTFPPDQSLFAAWDAFNTGFTSWKTRDSEILINTMVAQYAELDLIWQKVKDDTEEHVALDFKEGIRENQLLLLVRIRRLAGERTRALIRQAVKDARRTRLPRKEKGDTRPRATPTASDGNSVSTEVESSSVSSGAAAASLSPAGSPQPPETPAGVADQSRFPPMENGAIFSNRQVVHELALDKDFKLGARKKGGLERVVDEQARRAFWDAMKEDVLERGELAKWIPGMAGSVKEKLLRLLESTGSLHRMISDSIDIQIIEQQCRAGSYDYEKFFSFVLSILPKICSPARDEDVQALINDTTSNYIHRLQRLFDVLELLQLDHANFLLMMSAQYVIPEAVPYERRLFAADLEAGRTTLTQTKRWLLKAKEEKLQEARSRDPEGVNHPSNRPPSTGIYNHAFASLVMSLGGPLTLQEVPETFHLDIERIRTYREEFRSLVLGAAMGLTIKNLLHRDVRSSWKPLKDRISTDLSSETPLEPLAIAQDLCTFLRETVATPKQVVSHVTSAITRIAARSGSQEHQDPVVRVVSNRLRGFVLARLNAGTSKEKVRLATGGGDTLTSFGMAEWISEVGAFVERSVALTDLNRNCYAVWYDGILA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4E0</accession>
    <name>D5G4E0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00004066001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835262.1">
        <property type="nucleotide sequence ID" value="XM_002835216.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4E0"/>
    <dbReference type="GeneID" id="9185008"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004066001"/>
    <dbReference type="HOGENOM" id="CLU_1670658_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4E0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ79383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="158" mass="18348" checksum="4BF5100AEC833C93" modified="2010-06-15" version="1">MVIKFYFTISSFILRYLRPGPPSTHGPPPSMAHVGLNHNPKDWPNPSLTLPNPNTYPTGIGLVARYDNYYDNYDTYKYDMLRTSTPRQLLTAIAAREGERKESIKEEKGSGTCQDISEISIFGEREQQRRRRRRLITTTAGYWYHTIALPRLECWADY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4J2</accession>
    <name>D5G4J2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00004175001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429987">
        <property type="protein sequence ID" value="CAZ79435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842515.1">
        <property type="nucleotide sequence ID" value="XM_002842469.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4J2"/>
    <dbReference type="GeneID" id="9184067"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004175001"/>
    <dbReference type="HOGENOM" id="CLU_321374_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4J2"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="286"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="531"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="555"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="600"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="629"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="677"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="691"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="705"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="761"/>
            <end position="776"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="902" mass="99075" checksum="F1ABB75BD72DB4B5" modified="2010-06-15" version="1">MNLRCGWQPGDVYAPVIFETLEDATRAYKELAGRKLGRSTVWLSWEMPNTIVYIMNIKPMTKSYRILSLLQGYRLYGNLVRRPHMMIAAFRNPEEAEIAVSRLQGTRVDGELLQFSILRGQHASEFVGYDRSLSCYMCLRTFRSGTRAVSIIATPPLRLSLAVESPVYYELSFNLGENPQITLHDSQVLPTVTSRDIALRIRCAGHTAIRAGPQGGASNFVRFMNVFREDISRSGGMMDVGVVLEDPLDALKVETFFGESVPPASRVIPEMGGELRSYYGVTAGESSGVQENGGQRGNVLYSSAFNKPEPSRRASAGSTSDRPSCPGVNGTPLGRRANRKSKPHDSANTRGMGGERVLPIGLIKPGWLSPGYPPPDPSGASEVFAYSDPQPKVRQFPFSSTQPPASTMRPLIFGQFQQGPQLLPGGGQKTLTDVLKGSENSEKRAGDIGVAVGAMEDRWVDALPERMDGDGNGLFQSEAHIKRSSSDSASSSWSFLHRSYNGQAISSSSSAPVGSVGESVTQAFGQMHYTDDSNSEATESAPAEIVDLTVGNGKEGGRFEKSGNPRKERRKIPIVDSSDGDSRSELGSRAAMAWREGSTRKKKKSKRKEKVPSDEGMASGSGGEQPAVQRRRHKKGKRGKGKEIAHSDGSMEFQSDQDRPGPSNPRKESRQKKISKQVESPSGYESPTSSKIKKKKKKKKRKAEEAEEVQEGTDVDMDASDPPRRLTRKRRGGRQLSVSESEDAIQPLIEGISETHVARGPSTSRLKSPADTSSSDPKTKKRKRNRTSSLSPILDPIPPPPPADLSSSPARPGESIDMACRRHDIEGEIHRINLQQHELSVRELDLRHELVKLRYQARVEAGAQGSTYANCMCSACGEYGHKKNNRAKCKKHEKYKDWYPER</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4L0</accession>
    <name>D5G4L0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00004204001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429988">
        <property type="protein sequence ID" value="CAZ79453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4L0"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004204001"/>
    <dbReference type="HOGENOM" id="CLU_2741882_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4L0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ79453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="71" mass="7925" checksum="807F235155ED9CEB" modified="2010-06-15" version="1">MVRANHGDLWYVFGRAGIARVKHWQRPGAGDTCASTGAECLAAYSAYIWQRLENGCCGHRLFLSRPFGPNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4L9</accession>
    <name>D5G4L9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00000004001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429989">
        <property type="protein sequence ID" value="CAZ79462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835305.1">
        <property type="nucleotide sequence ID" value="XM_002835259.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4L9"/>
    <dbReference type="FunCoup" id="D5G4L9">
        <property type="interactions" value="188"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G4L9"/>
    <dbReference type="GeneID" id="9188399"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000004001"/>
    <dbReference type="eggNOG" id="ENOG502S21G">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025891_1_0_1"/>
    <dbReference type="InParanoid" id="D5G4L9"/>
    <dbReference type="OMA" id="GPSMIHH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013226">
        <property type="entry name" value="Pal1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28307">
        <property type="entry name" value="PROTEIN PAL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28307:SF2">
        <property type="entry name" value="PROTEIN PAL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08316">
        <property type="entry name" value="Pal1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="338"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="366"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="236"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="425"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="458"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="480"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="522"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="535" mass="58897" checksum="A362087CB9AC383A" modified="2010-06-15" version="1">MSLVQPPVPDRRPAPSIELGTNNPFRRRLSNTPSSPASATNERFSFVSTGGRPQSRNPFLDVFEDDFFAAERPSAAHKANSFDSSSALRPQFSGAAAELFENLTITDHSGDTNTSNMYGRSDGRDYMPPPPYRPQQKHRQLSNGHHHSRSSDEKGAQLIPIDSTPRNPPPRQRPPPNGRFVDRPRDMDRELRRRPRRNSDSSVIDVHEAEQKDRERRERRHREQRSRDQGSGSGSGRKERDDRVRREKDRKKNGAPLDVIDKLDVTGIYGSGLFHHDGPFDACNPHRNAKNSRRLAPVHAFPADSANNALSGFGPVSAKADHSHVFGNRDPEAFNDFTASGRRPSAPSELGSTKAIGAFDPKKNVDIIHGDETPGLGTSTFLDGTPASRAAVQKSESTDDRPKSPNGMNNGGGLQRKKSLAQKIRSISQNRTTDGPVQSHRRPPPPPPNRSPTDGAYSPTTPAGNSSHNNNPFFSDYDGAYDRKGETISVSERRAPGSPRKPSLGRSQTGDGKPGENGGLMKRVRSLSKPKRRNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4N0</accession>
    <name>D5G4N0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00000018001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429989">
        <property type="protein sequence ID" value="CAZ79473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835316.1">
        <property type="nucleotide sequence ID" value="XM_002835270.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4N0"/>
    <dbReference type="STRING" id="656061.D5G4N0"/>
    <dbReference type="GeneID" id="9188410"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000018001"/>
    <dbReference type="eggNOG" id="KOG1091">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019939_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4N0"/>
    <dbReference type="OMA" id="SPWQTLR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000038">
        <property type="entry name" value="TBC1 domain family member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000031">
        <property type="entry name" value="TBC1 domain family member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF337">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="3">
        <location>
            <begin position="72"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="419"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="588"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="702"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="614"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="634"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="657"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="717"/>
            <end position="736"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ79473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="761" mass="84528" checksum="C886A043E56788BA" modified="2010-06-15" version="1">MRPLQETRYHLLSSPPPTQHCLQLLKPKRRLGTIRKLWESLFKGHSSISSLRTATTDSIYKNNGNSGSTEGPCGVGLRSVCWKLFLLFPGFKPEAWSRTIRYERAAFEELKKVHVSPFEKAADFGEGIDPLAEVAENPWEQFRKDESLRKEILQDIERCMPENTYFRDPTIQNSLLNILFIYCKLNADVSYRQGMHEIVAIILWVVSCDAISTAAGTEGQEEEGKPVADENIMVECLDHQFIEHDTFSLFQVVMRSAKAWYEIGEEALDSVKGRGRNENRNSSPIVEKSKYIHEHLLMAVDPELAEHLKALDVLPQVFLIRWIRLLFGREFPFEELLEVWDALFAEDPNLQLVDHICVAMLLRVRWQLMEADYSTALTLVLRYPSPTAPDLPATFVADAIYLRDNPTFAGGKHIVVKYSNRTPTPPAQKQRAPKLSRKNSPLTPPARFLQTGQLESIVQDVAKNMLSQGERWGVNRAVRDAVGDIKKNVQGLQQSQHQQWAAQESAREAELVRRSRELSKKLKADDDRRKKLERLLQLSIEELEKNGLTTEQKKVSLVRLNHVRECLLDGTKTVDYTASAAPLPKILPITTSVPTTPPSPAANPPLSGRAPPSSICTIHTPTSTVSQPTQKPKSAPPSPPGSTPPPKPQKVPTSPQGSFVKTSFKSNSDPDFLTHRPRASLAQSSFAWMLGDDPAAKTKTAFVSASARKSGNANSNHGGGGGGGSGGAAGADGVGNGVRVDATWTPDVDEGFDLGRLRRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4Q5</accession>
    <name>D5G4Q5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">T-complex protein 1 subunit eta</fullName>
            <shortName evidence="9">TCP-1-eta</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">CCT-eta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00000052001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. Known to play a role, in vitro, in the folding of actin and tubulin.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Heterooligomeric complex that forms two stacked rings.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429990">
        <property type="protein sequence ID" value="CAZ79491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835334.1">
        <property type="nucleotide sequence ID" value="XM_002835288.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4Q5"/>
    <dbReference type="FunCoup" id="D5G4Q5">
        <property type="interactions" value="1357"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G4Q5"/>
    <dbReference type="GeneID" id="9188427"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000052001"/>
    <dbReference type="eggNOG" id="KOG0361">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008891_7_1_1"/>
    <dbReference type="InParanoid" id="D5G4Q5"/>
    <dbReference type="OMA" id="HRKGNTW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03340">
        <property type="entry name" value="TCP1_eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000017">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.260.10:FF:000022">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000006">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012720">
        <property type="entry name" value="Chap_CCT_eta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02345">
        <property type="entry name" value="chap_CCT_eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 8" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="523"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="545"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ79491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="555" mass="60461" checksum="B59A0975A8A1F5F8" modified="2010-06-15" version="1">MAFAGQTPTIIVLKEGTDASQGRGQIISNINACLAVQSTIKSTLGPYGGDLLLVDANGRQTITNDGATVMKLLDIVHPAARILTDIARSQDAEVGDGTTSVVVLAGEILKESKTFIEEGVSSQIIIKGFRTAARLAENKIKEIAVRIEKGDRRDTLLKLATTAMSSKLIKSNSSFFSKMCVDAVMSLDQELLSEKLIGIKKVPGGGLQDSLFVNGVAFKKTFSYAGFEQQPKSFKNPKIVCLNVELELKSEKDNAEVRVEQVSEYQAVVDAEWQIIFQKMEALANTGAKIVLSKLPIGDLATQYFADRDIFCAGRVASEDMDRVIQAVGGAIQSTCSDIREEHLGSCEHFEERQIGGERYNFFEGCPEAKTCTLVLRGGAEQFIAEVERSLHDAIMIVRKAIKNDYIVAGGGAIEMELSKYLREYSRTIAGKQQLIIGAFAKALEIIPRQLCDNAGFDATDILNKLRMRHGKGETWAGVDIVNEGIANNLERFVWEPTAVKVNAIQAATEAACLILSVDETVKNQESEQPQSGPPMPRGAAQRALRGRGRGMPRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5J9</accession>
    <name>D5G5J9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00004375001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835635.1">
        <property type="nucleotide sequence ID" value="XM_002835589.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5J9"/>
    <dbReference type="GeneID" id="9183778"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004375001"/>
    <dbReference type="HOGENOM" id="CLU_1679241_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5J9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="13"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="24"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="70"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="117"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="157" mass="15689" checksum="3F3B5E535C9C774A" modified="2010-06-15" version="1">MCFGFTKIWRRKLKKSTANAAEKPVSASTSTTSPPTPVPVYKPPPNIRLDGHITAAAAMDNTPVTKNEKPQTTTPPSKPTPSTTYLSAPSPSYSSGIRSHSTLSPGSHFGETTPSISCSGGHHSSSYHSSSGSSSSGGGGGGGCSSSSSGGGGGSGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5L0</accession>
    <name>D5G5L0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ubiquitin-like modifier HUB1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00004394001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835646.1">
        <property type="nucleotide sequence ID" value="XM_002835600.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5L0"/>
    <dbReference type="FunCoup" id="D5G5L0">
        <property type="interactions" value="318"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G5L0"/>
    <dbReference type="GeneID" id="9183789"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004394001"/>
    <dbReference type="eggNOG" id="KOG3493">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_156193_2_0_1"/>
    <dbReference type="InParanoid" id="D5G5L0"/>
    <dbReference type="OMA" id="GMSLEMQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031386">
        <property type="term" value="F:protein tag activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000753">
        <property type="term" value="P:cell morphogenesis involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033120">
        <property type="term" value="P:positive regulation of RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043687">
        <property type="term" value="P:post-translational protein modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01791">
        <property type="entry name" value="Ubl_UBL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000052">
        <property type="entry name" value="Ubiquitin-like protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039732">
        <property type="entry name" value="Hub1/Ubl5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13042">
        <property type="entry name" value="UBIQUITIN-LIKE PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="3">
        <location>
            <begin position="2"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00014108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ79803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="73" mass="8374" checksum="E90B12539FB2CCF8" modified="2010-06-15" version="1">MIEVICNDRLGKKVRVKCEPNDTIGDFKRLVAAQIGTSAEKIVLKKWYNTFKDHITLADYEISNGTSLEMYYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6H5</accession>
    <name>D5G6H5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein ARV</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00004485001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Mediator of sterol homeostasis involved in sterol uptake, trafficking and distribution into membranes.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Regulates also the sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Golgi apparatus membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ARV1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="FN430009">
        <property type="protein sequence ID" value="CAZ80118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835961.1">
        <property type="nucleotide sequence ID" value="XM_002835915.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6H5"/>
    <dbReference type="FunCoup" id="D5G6H5">
        <property type="interactions" value="25"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G6H5"/>
    <dbReference type="GeneID" id="9181203"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004485001"/>
    <dbReference type="eggNOG" id="KOG3134">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057366_0_0_1"/>
    <dbReference type="InParanoid" id="D5G6H5"/>
    <dbReference type="OMA" id="MLDMNVK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032366">
        <property type="term" value="P:intracellular sterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097036">
        <property type="term" value="P:regulation of plasma membrane sterol distribution"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006665">
        <property type="term" value="P:sphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016125">
        <property type="term" value="P:sterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007290">
        <property type="entry name" value="Arv1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14467">
        <property type="entry name" value="ARV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14467:SF0">
        <property type="entry name" value="PROTEIN ARV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04161">
        <property type="entry name" value="Arv1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 10" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="138"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="171"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU368065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ80118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="275" mass="30264" checksum="43473ECD50D557DC" modified="2010-06-15" version="1">MSSTPPTPPKNLYICIECSSPVQSLYTAYSSADDRSLGRGVRLTQCPRCKRFADKYVEHDFVVLFIDLVLIKPQVYRHLLFNRLGREDDRFEARKASAMKEGTSLIISAYSFLANIPTDPLNTSAFGILTQRPIVVQYLFFLLLCLTESLLFHLTIRLISQYLLGATRPNAVSTALIVSSCTKLFPILMVIWNYEIPAAAKSVGWAVVVNNVEALRISLGIDYFRAAGLAGAGAVVRAVASQTILGWTGLLGDNDRWGGMGVGIEEWVGRLGLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6V8</accession>
    <name>D5G6V8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Flavin-containing monooxygenase 1</fullName>
            <ecNumber evidence="14">1.14.13.148</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Dimethylaniline monooxygenase [N-oxide-forming] 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Dimethylaniline oxidase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Trimethylamine monooxygenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">GSTUM_00002301001</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Broad spectrum monooxygenase that catalyzes the oxygenation of a wide variety of nitrogen- and sulfur-containing compounds including xenobiotics. Catalyzes the S-oxygenation of hypotaurine to produce taurine, an organic osmolyte involved in cell volume regulation as well as a variety of cytoprotective and developmental processes. In vitro, catalyzes the N-oxygenation of trimethylamine (TMA) to produce trimethylamine N-oxide (TMAO) and could therefore participate to the detoxification of this compound that is generated by the action of gut microbiota from dietary precursors such as choline, choline containing compounds, betaine or L-carnitine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>N,N-dimethylaniline + NADPH + O2 + H(+) = N,N-dimethylaniline N-oxide + NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:24468"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16269"/>
            <dbReference type="ChEBI" id="CHEBI:17735"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.14.13.8"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:24469"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>hypotaurine + NADH + O2 + H(+) = taurine + NAD(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:74111"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57853"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:507393"/>
            <dbReference type="EC" id="1.14.13.8"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:74112"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>hypotaurine + NADPH + O2 + H(+) = taurine + NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:69819"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57853"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:507393"/>
            <dbReference type="EC" id="1.14.13.8"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:69820"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>trimethylamine + NADPH + O2 = trimethylamine N-oxide + NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:31979"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15724"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58389"/>
            <dbReference type="EC" id="1.14.13.148"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:31980"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FMO family.</text>
    </comment>
    <dbReference type="EC" id="1.14.13.148" evidence="14"/>
    <dbReference type="EMBL" id="FN430017">
        <property type="protein sequence ID" value="CAZ80251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836094.1">
        <property type="nucleotide sequence ID" value="XM_002836048.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6V8"/>
    <dbReference type="FunCoup" id="D5G6V8">
        <property type="interactions" value="595"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G6V8"/>
    <dbReference type="GeneID" id="9182475"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002301001"/>
    <dbReference type="eggNOG" id="KOG1399">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006909_8_2_1"/>
    <dbReference type="InParanoid" id="D5G6V8"/>
    <dbReference type="OMA" id="CCTGYDI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034899">
        <property type="term" value="F:trimethylamine monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000159">
        <property type="entry name" value="Dimethylaniline monooxygenase [N-oxide-forming]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000960">
        <property type="entry name" value="Flavin_mOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050346">
        <property type="entry name" value="FMO-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23023">
        <property type="entry name" value="DIMETHYLANILINE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000332">
        <property type="entry name" value="FMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00370">
        <property type="entry name" value="FMOXYGENASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="8" id="KW-0521">NADP</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00045957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00049443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="CAZ80251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="518" mass="58260" checksum="36673EF626916BBE" modified="2010-06-15" version="1">MVLRVGIIGAGISGLVSIKQCLEEGVEPVCFEALGHFGGQWHYTDPDPHTGEVASSMYRSVVINTSRETMMMSDFPMDPNMYAMYTHNSKVQQYFESYAEFFKLQPYIRFNHRVRRAYPAGDGKWTVEVESGGEVTVDTYDAVFVCTGHHSTPNMPDWQDVEKFEGELVHSHYYRDTVKFQGKNVAVVGVGNSGADISAELSSCTKSTHLITRSGTWVFPRFLLGEPYEYLGSRFMLNMVPRSVAIAGMQWALNYTLGTIPKELKPEHNLLGAHPTIRSDLIERVRTGTVTAHRGSIKRFTKKGVELTNGEIVEPLDAVVAATGYTLNFPFLPKGIVQSDEDKDGKENWANLYRLIVAPGHPGLYFIGLCQALGALMPVAEMQARWAISVLKNEIPLPSPERMSEDIAAYQNDLKKVFVRSPRHTLEVEFFPYMDLLAQDLGVHVTLGRFFSTFGVWQGIKTARSAYFGPPSAVQYRLFGKGRKEELARLVLQRLARKRNEEMSVEEMAEVERYRVGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6W1</accession>
    <name>D5G6W1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00002310001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430017">
        <property type="protein sequence ID" value="CAZ80254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836097.1">
        <property type="nucleotide sequence ID" value="XM_002836051.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6W1"/>
    <dbReference type="STRING" id="656061.D5G6W1"/>
    <dbReference type="GeneID" id="9182478"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002310001"/>
    <dbReference type="eggNOG" id="KOG0158">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_14_2_1"/>
    <dbReference type="InParanoid" id="D5G6W1"/>
    <dbReference type="OMA" id="ECMDHMA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050121">
        <property type="entry name" value="Cytochrome_P450_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305:SF166">
        <property type="entry name" value="CYTOCHROME P450 12A4, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="16"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="513"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602403-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ80254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="577" mass="65390" checksum="5EC67DD217E17CEF" modified="2010-06-15" version="1">MIDSEYFLNGISNMEYLTAAICAFGALIVWKLISAIFFSPLSHIPGPLVTAVTPHYINFLSALNQRTVGTYSLHKRYGPIVRLSPTEISILSPQAIKEVYSSPHYTKYTPLYSIFTHFGAQNSFTSCTREEHGWRRKAVSEPYSLSFVLKDEAATGKVLKAVKDYLGFVESDRRVDIYNANTFYATDVVTGKIFGLEASMKTLAGNEEHREIVLGHYARTRRTQVWMYIEFPLIMNLFEWFAFYTGRVWSWIHGEEIEGWEMVSQAQRWGWDAYMDAKSNSREGTVAGRLAKLVQEGGSGEEAWDDRGAVSEVMDQMLAGMDTTGDTLSFLMYQISLPESRSVQKRLHSELLNAFPKSAEMPGLGWPHSMDVQSLPHETILKVLNLPYLDAALKETLRVYSAIPITLPRVVPASTTTDRISGHRNGRILEGKFIPAGTTIGTLAYGIHRDEVFAVETKQKDEPGDVDSFVPERWLINGGIEGKLTPDELEQEKQRIRTMEARLWAFGSGGRNCVGRHLSILEMKLLLATIYSRYQTQVTPNSGVKITHNRWDQRRTFRDVLPFRRVDGVVTFTPYEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5G764</accession>
    <name>D5G764_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00002483001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430022">
        <property type="protein sequence ID" value="CAZ80357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836166.1">
        <property type="nucleotide sequence ID" value="XM_002836120.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G764"/>
    <dbReference type="FunCoup" id="D5G764">
        <property type="interactions" value="18"/>
    </dbReference>
    <dbReference type="GeneID" id="9185290"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002483001"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006466_0_0_1"/>
    <dbReference type="InParanoid" id="D5G764"/>
    <dbReference type="OMA" id="LNHNPKQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051059">
        <property type="entry name" value="VerF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40626">
        <property type="entry name" value="MIP31509P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40626:SF11">
        <property type="entry name" value="ZINC FINGER PROTEIN YPR022C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="68"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="98"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="120"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="150"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="295"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="633"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="17"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="43"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="150"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="186"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="242"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="261"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="297"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="314"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="649"/>
            <end position="661"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ80357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1062" mass="116138" checksum="8F7DC259DE8845B6" modified="2010-06-15" version="1">MSTAGHHQAAGSYQQPREMEEKARGRERNRDGMMDGSGGQVIGGPLSSSSDPPSRKRRRSRKGLDKKFECPAEGCGRSYSRAEHLYRHQLNHQPKQIYFCDFPDCKRSFVRQDLCMRHRDRHTNRPGSQLQRKDSLLNNPAGAAARNILSERGGSMSKGSSSPESPNGPGLMMKSGEDESPIMTPNGVSMNMTASPDTSLSSATLPNSSKASPVDVNTLYGGGQQRPGLSSSSKSDFARPPLQTSVGPNQTIGGMNMQPLSASSVSSGGHFSAPLTSATSAGSLAATTGTSVGPAYNPQTSFTPFSLPQPAYPNLSQSNASNQGQNQYITSPPTSSSSVGASNSIGGVTTTDVDFESMNVPFTLPVFGNDGYTRSPNWMADDFATWFFEGTQLPGGSRTSSIGGNYVDSLTMGPQNLYLGGPEFQTHNYYPHPLQPQHPMAVTSLIAPPSPPQESLLSEHKRQDLMDHIENYPEMSDLSQRTGSLVLGSAEDDSHALSLHMMQSYISSYWLHFHPQLPILHRPTFSADKTPSLLLLTMMAIGASLIGTKVDPTVAQAATDLSVILAWNLRWQIFSDMAFRPPAKLWVFQALLLLETFEKMFASRALHERAHIHHATTLTLMRRGSSLTGRCAYDSPQSGRDVKSNSDNGSAAFSGQSSGSAPVEDAWNRWIVSEATKRAAFAAFVLDSTHATMFGHSAVLVAHEMRLQLPCDETLWAATNGAEVFRIEHSLLQAGVKPTTFLDGLKKTLNGQNVRTNSFGRTILMAGLLSVSWHMAQRDLQVTSLGAVAQLGGKDKWRQSLTRSFDYWKKDFDSALEGIRAGSGTYEGSNYCLPSTEDVEHETTFESRNVMHHLAHMSMHVDIVDLQIFAGAKRLLGRTVIPSDAAQVRKRVKDWAPSARARDATFYALQFLCQVLLPEERVDMPYTADDDDLGAPRKLSSATTGIPIYSARDDHLLNRPWVLYYASLVVWSYGFALEGQVQVPRSYFSTPAARHQDMRTFLRRVGGVRSPEELANVRDKNACLGLLLTLHGMFAKARWELLVEASRLLDQCVSISTGEIQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7A4</accession>
    <name>D5G7A4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00002414001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430023">
        <property type="protein sequence ID" value="CAZ80397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836206.1">
        <property type="nucleotide sequence ID" value="XM_002836160.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7A4"/>
    <dbReference type="FunCoup" id="D5G7A4">
        <property type="interactions" value="616"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G7A4"/>
    <dbReference type="GeneID" id="9185295"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002414001"/>
    <dbReference type="eggNOG" id="KOG2630">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023273_0_0_1"/>
    <dbReference type="InParanoid" id="D5G7A4"/>
    <dbReference type="OMA" id="LQGMVWE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043874">
        <property type="term" value="F:acireductone synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01629">
        <property type="entry name" value="HAD_EP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023943">
        <property type="entry name" value="Enolase-ppase_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01691">
        <property type="entry name" value="enolase-ppase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20371">
        <property type="entry name" value="ENOLASE-PHOSPHATASE E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20371:SF1">
        <property type="entry name" value="ENOLASE-PHOSPHATASE E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ80397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="256" mass="28087" checksum="8223338C1BFB51F3" modified="2010-06-15" version="1">MPPKTILINIEGPPRLHPPPKKKKFSQLTNLGTICPITFVKEILFPYALAALPGYLSKNWTTTNPLIAAFPIANTTTPAALINYVQAQTIQDSKSPALKALQGHLWRAGYESGELVAPIYPDVLPAVRAWRSQTPRAVRVYVYSSGSVRAQELFFHYSCEGDVRGLFDGFFDTRVGAKVEAESYRKILRECEEGGEGEGWVFLSDHVGEVRAAMEAGMIGGVVVREGNAPLSEEDKVGMWVIGGGFTEVTEKLFAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7N2</accession>
    <name>D5G7N2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00004643001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C15 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430031">
        <property type="protein sequence ID" value="CAZ80525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836334.1">
        <property type="nucleotide sequence ID" value="XM_002836288.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7N2"/>
    <dbReference type="GeneID" id="9187004"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004643001"/>
    <dbReference type="eggNOG" id="KOG4755">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043960_1_0_1"/>
    <dbReference type="InParanoid" id="D5G7N2"/>
    <dbReference type="OMA" id="KLAYNHK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.20">
        <property type="entry name" value="Peptidase C15, pyroglutamyl peptidase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016125">
        <property type="entry name" value="Peptidase_C15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036440">
        <property type="entry name" value="Peptidase_C15-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23402">
        <property type="entry name" value="PROTEASE FAMILY C15 PYROGLUTAMYL-PEPTIDASE I-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23402:SF1">
        <property type="entry name" value="PYROGLUTAMYL-PEPTIDASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53182">
        <property type="entry name" value="Pyrrolidone carboxyl peptidase (pyroglutamate aminopeptidase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ80525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="247" mass="27441" checksum="AE9BF619814447A0" modified="2010-06-15" version="1">MTDQTPIKKKNITIYLTGFGPFRTHTHNSSWAIISSFQPRTTIATETHTITLLPHPHWLKVAYSTVTSEVPRLHTATDVAFDYILHVGLGKAGEYRLERRAFEEGYVFGDVDGKVPGGADAAPDKGIMQSESAEGREPGGQEFGCALDVRWIVEEGVERFQPEETPDEELPIKVSDDAGRFLCEFTYYTSLKAACEKEGNGKLKSEKVLFMHVPPLTKPFGIETGRRVLIEVAKAMVKAGEERKVIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7P7</accession>
    <name>D5G7P7_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00004671001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="FN430031">
        <property type="protein sequence ID" value="CAZ80540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836349.1">
        <property type="nucleotide sequence ID" value="XM_002836303.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7P7"/>
    <dbReference type="STRING" id="656061.D5G7P7"/>
    <dbReference type="GeneID" id="9187019"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004671001"/>
    <dbReference type="eggNOG" id="KOG0591">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_63_23_1"/>
    <dbReference type="InParanoid" id="D5G7P7"/>
    <dbReference type="OMA" id="LEMEIFK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08217">
        <property type="entry name" value="STKc_Nek2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051131">
        <property type="entry name" value="NEK_Ser/Thr_kinase_NIMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899">
        <property type="entry name" value="CAMK FAMILY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899:SF10">
        <property type="entry name" value="NIMA-RELATED KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="9"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="442"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="443"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="459"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ80540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="671" mass="74696" checksum="E1310E8A282F5198" modified="2010-06-15" version="1">MPPLTSADYDVIGTIGHGCFGTIRKVKRKQDGIVLARKEISYVKMGSTAKNQLASEFEILACLDHPNIVKYYHREHIKSESSVHLYMEYCGNGDLSGLIKKAKESGNSFPESFIWTVVTQLTTALYRCHHGIDPPELSDLFSYKNTTPPTPRTESEERDLIKVLHRDLKPENIFVMADNSIKIGDFGLSKKLAPGNMFAETYAGTPYYMSPEISSGRPYTVKSDIWSMGCIIYELCTQELTFTSNTLPGLINLIGKGKYKPIPVRYSKRLRDLVARCLSVDPDSRPDSAELLEDETIKVFRRELQILEVSKLLKRREEDCLRKEAYLEANAKTLEQRFIERRQRLEREINGKIRSDWEIRAESVIKEKVEEAKFLMRGELEAEYREKFNQTVELEVQARIARMDLVPRSYALAGGEPFTESAFNQKIEVEVQKRIAQMDLVPRTTSLSNSNSRSDSGYAGSTSPTRANFPNLPPDSPADIMMESPSPHAVATPGQKLSTARVLHGPPPRMPFGHVSYTFADFTNTLSHGVQTVWGNQPATQPETEDNTSSNLFGMQPLKSPTNGQLSLATKRNISTNANGSPTRVAPRARYGLQRAETTGGAVGSARLMAGFGVNGTGSTDDIATVKPNTTGAVKPKSLIEINLEARLKFGPPVVWAKENPADLPSPFKKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7V2</accession>
    <name>D5G7V2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00002667001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430032">
        <property type="protein sequence ID" value="CAZ80595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836404.1">
        <property type="nucleotide sequence ID" value="XM_002836358.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7V2"/>
    <dbReference type="STRING" id="656061.D5G7V2"/>
    <dbReference type="GeneID" id="9181414"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002667001"/>
    <dbReference type="eggNOG" id="KOG4169">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_13_3_1"/>
    <dbReference type="InParanoid" id="D5G7V2"/>
    <dbReference type="OMA" id="MYTTHLA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44229">
        <property type="entry name" value="15-HYDROXYPROSTAGLANDIN DEHYDROGENASE [NAD(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44229:SF4">
        <property type="entry name" value="15-HYDROXYPROSTAGLANDIN DEHYDROGENASE [NAD(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ80595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="400" mass="43771" checksum="0065D348354B58D2" modified="2010-06-15" version="1">MSNEVVIVNLRSNLIFDFWSNLSAPIVPYRITTQLYSLYLGPRTLASTNLHSAQRKAFISYSSMFNTSQTLSTTDVYYSTTPIDLKAPYNVTTAGRKTILITGGARGLGEGMLRKFAKLGATVIVGDLNESRGESVIAKVRKSSDNENVHFLPLDVTSYTSQCEFFRSARELSPTKKITTVIANAGVAELGSFIESSPTKIPLDVDPPEPDMTTVDVNFKGVLYTTKLALHHLRSSPVGQRELILVGSMSSFTSGPMMAVYSATKHGVLGLFRSIRLFPSKRDGVRINMICPYFVDTPIVPIGGKVMLAGLEIAVLDDVVDAVSRLVCDEGIAGRCLAIAPRQAGGVMEVGVQEMENVDAFCKRVVIAMNMRSKMEIFTKFLVDILWLLVVGPILRLVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7Z0</accession>
    <name>D5G7Z0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00002737001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430035">
        <property type="protein sequence ID" value="CAZ80633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836442.1">
        <property type="nucleotide sequence ID" value="XM_002836396.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7Z0"/>
    <dbReference type="GeneID" id="9184106"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002737001"/>
    <dbReference type="HOGENOM" id="CLU_3299692_0_0_1"/>
    <dbReference type="InParanoid" id="D5G7Z0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="40" mass="4684" checksum="AC5643067533AEA6" modified="2010-06-15" version="1">MHTKAHFVIGSTNNKEEESSTELKPQEDPQYYSSKRKTEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>D5G871</accession>
    <name>D5G871_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00002913001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430041">
        <property type="protein sequence ID" value="CAZ80714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836523.1">
        <property type="nucleotide sequence ID" value="XM_002836477.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G871"/>
    <dbReference type="STRING" id="656061.D5G871"/>
    <dbReference type="GeneID" id="9188316"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002913001"/>
    <dbReference type="eggNOG" id="KOG1570">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2724039_0_0_1"/>
    <dbReference type="InParanoid" id="D5G871"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ80714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="72" mass="8294" checksum="BEB108E476E812EA" modified="2010-06-15" version="1">MSKITVAGVRGNVKELLEYSTNEKKRNFLETVELQIGLKNYDPMLAPGSPAKHVYLYPWRPARHRSRQAPQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8C5</accession>
    <name>D5G8C5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00004741001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430042">
        <property type="protein sequence ID" value="CAZ80768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836577.1">
        <property type="nucleotide sequence ID" value="XM_002836531.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8C5"/>
    <dbReference type="STRING" id="656061.D5G8C5"/>
    <dbReference type="GeneID" id="9188370"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004741001"/>
    <dbReference type="eggNOG" id="ENOG502S704">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074390_1_1_1"/>
    <dbReference type="InParanoid" id="D5G8C5"/>
    <dbReference type="OMA" id="QWEVADE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1230.10">
        <property type="entry name" value="MTH938-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036748">
        <property type="entry name" value="MTH938-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007523">
        <property type="entry name" value="NDUFAF3/AAMDC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21192:SF2">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX ASSEMBLY FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21192">
        <property type="entry name" value="NUCLEAR PROTEIN E3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04430">
        <property type="entry name" value="DUF498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64076">
        <property type="entry name" value="MTH938-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="204" mass="21738" checksum="479B33A981373A35" modified="2010-06-15" version="1">MRPFFLPTPRLLTLHPLPRPSPRPPKSQPLHTIPHPSPSRSPEPATSNSSLRGHNHDTPSPAPGLLGLDIYSDLPSPVNSVEALYDTHFLLASGIRTAPGSGVFLLNDFVFSWAPDVACKGGLVGFGEGSWGVLEVVWPKPELLIIGTGKRTLFLAPEDKKRIAELGVRVDVMDTGSAVAQFNLLATERGSGIAGALLARGFKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8Q6</accession>
    <name>D5G8Q6_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Acyl-coenzyme A diphosphatase SCS3</fullName>
            <ecNumber evidence="8">3.6.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">FIT family protein SCS3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SCS3</name>
        <name evidence="8" type="synonym">FIT2B</name>
        <name evidence="11" type="ORF">GSTUM_00003096001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Fatty acyl-coenzyme A (CoA) diphosphatase that hydrolyzes fatty acyl-CoA to yield acyl-4'-phosphopantetheine and adenosine 3',5'-bisphosphate. Preferentially hydrolyzes unsaturated long-chain acyl-CoA substrates in the endoplasmic reticulum (ER) lumen. This catalytic activity is required for maintaining ER structure and for lipid droplets (LDs) biogenesis, which are lipid storage organelles involved in maintaining lipid and energy homeostasis. May directly bind to diacylglycerol (DAGs) and triacylglycerol, which is also important for LD biogenesis. May support directional budding of nacent LDs from the ER into the cytosol by reducing DAG levels at sites of LD formation. May play a role in the regulation of cell morphology and cytoskeletal organization. Involved in phospholipid biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + H2O = S-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-4'-phosphopantetheine + adenosine 3',5'-bisphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:65568"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57368"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:156554"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(9Z)-octadecenoyl-CoA + H2O = S-(9Z-octadecenoyl)-4'-phosphopantetheine + adenosine 3',5'-bisphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:65564"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:156553"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>an acyl-CoA + H2O = an acyl-4'-phosphopantetheine + adenosine 3',5'-bisphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:50044"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:132023"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>hexadecanoyl-CoA + H2O = S-hexadecanoyl-4'-phosphopantetheine + adenosine 3',5'-bisphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:50032"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:132018"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Endoplasmic reticulum membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the FIT family. Fungal FIT2B/SCS3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.-" evidence="8"/>
    <dbReference type="EMBL" id="FN430050">
        <property type="protein sequence ID" value="CAZ80899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836708.1">
        <property type="nucleotide sequence ID" value="XM_002836662.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8Q6"/>
    <dbReference type="FunCoup" id="D5G8Q6">
        <property type="interactions" value="67"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G8Q6"/>
    <dbReference type="GeneID" id="9186267"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003096001"/>
    <dbReference type="eggNOG" id="KOG3750">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048143_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8Q6"/>
    <dbReference type="OMA" id="FTSWFFG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010945">
        <property type="term" value="F:coenzyme A diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140042">
        <property type="term" value="P:lipid droplet formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03231">
        <property type="entry name" value="SCS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019388">
        <property type="entry name" value="FIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046400">
        <property type="entry name" value="SCS3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23129">
        <property type="entry name" value="ACYL-COENZYME A DIPHOSPHATASE FITM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23129:SF0">
        <property type="entry name" value="ACYL-COENZYME A DIPHOSPHATASE FITM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10261">
        <property type="entry name" value="FIT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="80"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="110"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="212"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="186"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ80899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="267" mass="29863" checksum="8440BF66B102D698" modified="2010-06-15" version="1">MASPKPSAYQAEKLDDDDVHQPPSLSPTPPSPGLVLLPREIFIASIYPLTLIAGSAVSVFSPSSASTNFHFSHKRNPLNVFFVKYGWLWTTLVFLIHTSRLRSSLRVKALLRYAFATIWWILVTQWFFGPPIMDLLFWFTGGECQLVDTDPMRTKEEMGPVQMVFSSAACKVAGGKWSGGHDLSGHAFLLTHASLFLWSELSCSGVAGWESAGVLALLVMWWWMLLMTGIYFHTWLEKITGWIAAMLYAWALRSYPTVWSTLGVPGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8T2</accession>
    <name>D5G8T2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00004826001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the WD repeat PROPPIN family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430053">
        <property type="protein sequence ID" value="CAZ80925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836734.1">
        <property type="nucleotide sequence ID" value="XM_002836688.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8T2"/>
    <dbReference type="FunCoup" id="D5G8T2">
        <property type="interactions" value="379"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G8T2"/>
    <dbReference type="GeneID" id="9186285"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004826001"/>
    <dbReference type="eggNOG" id="KOG2111">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025895_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8T2"/>
    <dbReference type="OMA" id="GGPQCMC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080025">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070273">
        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010314">
        <property type="term" value="F:phosphatidylinositol-5-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048720">
        <property type="entry name" value="PROPPIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11227">
        <property type="entry name" value="WD-REPEAT PROTEIN INTERACTING WITH PHOSPHOINOSIDES WIPI -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21032">
        <property type="entry name" value="PROPPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="259"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="264"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ80925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="367" mass="39429" checksum="0515F171DD6122BB" modified="2010-06-15" version="1">MNTRPQIATTGRPQALSATFNQDNSCFSIGLDSGFCVYNTDPCELQISRSLGAGIGVASMLGRANYLALVGGGRSPKFPPNKVIIWDDIKQKAVITLEFRSEVHAVRLSRQRIIVVLIGTVHIYAFSSPPAREHVFETHDNPLGLVALSSKFLAFPGRTPGHVNIFDLATGIVSIIPAHSTPLSAITISPQDDLLATASETGTLIHVYSTATSRMIRELRRGIDKAAIFSLAISPSSSRLAVTSDKNTLHVFELPSLSSVSQTPARPSSSHSTASNAPSGDNRRYSMLSKLPLLPKYFSSEWSAAHAPFEGGGRGALGWIGEDSVVAVGIGGKTGEARWEKFVIVEAENGGGIECIREGWRRYLDND</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8X1</accession>
    <name>D5G8X1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Arginine N-methyltransferase 2</fullName>
            <ecNumber evidence="8">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00004878001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">S-adenosyl-L-methionine-dependent protein-arginine N-methyltransferase that methylates the delta-nitrogen atom of arginine residues to form N5-methylarginine (type IV) in target proteins. Monomethylates ribosomal protein L12.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 8">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the class I-like SAM-binding methyltransferase superfamily. RMT2 methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="8"/>
    <dbReference type="EMBL" id="FN430053">
        <property type="protein sequence ID" value="CAZ80964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836773.1">
        <property type="nucleotide sequence ID" value="XM_002836727.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8X1"/>
    <dbReference type="FunCoup" id="D5G8X1">
        <property type="interactions" value="359"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G8X1"/>
    <dbReference type="GeneID" id="9181247"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004878001"/>
    <dbReference type="eggNOG" id="KOG1709">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033831_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8X1"/>
    <dbReference type="OMA" id="YWVVDNY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019702">
        <property type="term" value="F:protein arginine N5-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017408">
        <property type="entry name" value="Arginine_N-MeTrfase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051038">
        <property type="entry name" value="RMT2/GAMT_Mtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026480">
        <property type="entry name" value="RMT2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32379">
        <property type="entry name" value="GUANIDINOACETATE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32379:SF1">
        <property type="entry name" value="GUANIDINOACETATE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038148">
        <property type="entry name" value="Arginine_N-mtfrase-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51559">
        <property type="entry name" value="SAM_RMT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RMT2" evidence="10">
        <location>
            <begin position="167"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="130"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="130"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR038148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ80964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="393" mass="43851" checksum="6C3DEF22FB2F794E" modified="2010-06-15" version="1">MATTISTPAPTDLNTTMLLLASADHNTSVLTSLLKTSSPNVQDPDSKRTPLHAAIAGCDKDDPPEKLRAAEDTLKLLLQNGAIWNDLDRDGETPGCVAYRLGLKGLYEVMVEAGVRAELILARLGGWEEIEDEDDEDGGGEAAEGAERAEGGGNGIGVAVEVVSEEKDVNSENYLKSELKYHEDKLLDSDNNSVMMEWERDIMERSVEALLPGDKPGRSIMNIGFGMGIFDTIAQKKNVARHIIVEPHKDVLRKMKEEGWDKKPGVMILEGRWQDVLGSLTQTGETMDAIYFDTFAEDYSQLKLFFSEYVIALLAQEGGRFSFFHGLGADRRISYDVYTRVVEMDLFGAGLETTWEAFELGESERRKDQAWEGVRRKYWTLGEYKLPICMFME</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>D5G984</accession>
    <name>D5G984_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00003193001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430056">
        <property type="protein sequence ID" value="CAZ81077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836886.1">
        <property type="nucleotide sequence ID" value="XM_002836840.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G984"/>
    <dbReference type="GeneID" id="9184363"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003193001"/>
    <dbReference type="eggNOG" id="ENOG502SE0J">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_872068_0_0_1"/>
    <dbReference type="InParanoid" id="D5G984"/>
    <dbReference type="OMA" id="WNIGRDG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="244"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="246"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ81077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="319" mass="34614" checksum="91D25152B9053762" modified="2010-06-15" version="1">MPILPQITPSDKYLYLSAGLLAIYAIYTTHSQLNKFRSRCVSKPKVVPPDAGVDQTTEDAIKLETLETLARGINVDIRNAALKIITDRATTDENIVHLLSQVRSSTPSQRLRSLRALRCCTYSTTLQKLCQKLTFSALVSCLNSTLPTDENPTGDPFSEKEALYILARLMGLYFVGREMLVDAGFVLWLARAKVPGYANVVEAVFDDEGVDLVDPSLVQIVHVLQESVEARGALEDAGFMLRARDAGGGGGSPDGGRPGEGSEGEREDGQLEALVRNVFMGIGRERAGSTNGTAEPALDELLDDEALVMAGWDEEVLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5G998</accession>
    <name>D5G998_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00003210001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430056">
        <property type="protein sequence ID" value="CAZ81091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G998"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003210001"/>
    <dbReference type="HOGENOM" id="CLU_2322052_0_0_1"/>
    <dbReference type="InParanoid" id="D5G998"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ81091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="99" mass="10740" checksum="EB1A8893EB5B5485" modified="2010-06-15" version="1">MADHSPSSRKSGVYATVPICISQIVLLGLRCFAHSLGCHFATTANTHGSFLCYGMQHTCPPSFPLVCWGNERNTASSLCWSRLRRCAVGSVHDPTCRGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9N1</accession>
    <name>D5G9N1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00004983001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430064">
        <property type="protein sequence ID" value="CAZ81224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837033.1">
        <property type="nucleotide sequence ID" value="XM_002836987.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9N1"/>
    <dbReference type="STRING" id="656061.D5G9N1"/>
    <dbReference type="GeneID" id="9185361"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004983001"/>
    <dbReference type="eggNOG" id="KOG1175">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_3_5_1"/>
    <dbReference type="InParanoid" id="D5G9N1"/>
    <dbReference type="OMA" id="VDNWWST"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050218">
        <property type="term" value="F:propionate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032387">
        <property type="entry name" value="ACAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43347">
        <property type="entry name" value="ACYL-COA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43347:SF3">
        <property type="entry name" value="ACYL-COA SYNTHETASE SHORT-CHAIN FAMILY MEMBER 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16177">
        <property type="entry name" value="ACAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acetyl-coenzyme A synthetase N-terminal" evidence="4">
        <location>
            <begin position="6"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="2">
        <location>
            <begin position="69"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="3">
        <location>
            <begin position="528"/>
            <end position="609"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ81224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="663" mass="72381" checksum="E5E1F095A1B31388" modified="2010-06-15" version="1">MDEQSKHYSESLRAPADFWHVHAQKLSWETQYSTPLRVDGKRKWRWFPDGRISTTYNLVTRHVLLGRGEERAIIWDSPVTGRKCVITYAELQEEVEVFAEVLRGLGVKEGDTVLVYMPMIPSCTIALLAIAHLGAIHTVVFGGFSPAECAKRILSARPKLVLTASCGIEGSRIIPYLPLVREAISKSNWKPQNALVLQRDELYVSLDKSLQELNWNSLVRAKTVEVGAAFPSCAPQYIIYTSGTTGQPKGITRNIGGHIVGLQYTIEHLFNIHAGDTIFTASDLGWVVGHSYILYAPLLIGAATVLFEGKPVGTPDARTFWRIISEWKVNVLSTAPTALRAIKREDPTLGMVREFDISSLRAIFLAGERSEPSIVTDYQNAIFGPSTSPSCKGLVVDNWWSSESGSPITGISLGFNALPSPIKPGSAGKPMPGWDLKVVNDAGVPLLAGQNGNIVLATPLGPTGLTELWCDMEAARFHNAYFARFAGKGEWFDTGDAGFLDRDGYVHVMSRTDDVINVAAHRFSTGAIEEAILSHVAIAEAYVVGAPDPLKGHVPFAVVVLKPSCSSSPPSEEVLLKEVNGYVRTEIGPIAVLRGVVVVDKVPRTRSGKTLRRVVRAVVENAVRGEFGKEVELPATVEDGSVVVGLRERVEGWFLDRGIKARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5GB31</accession>
    <name>D5GB31_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00005421001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430086">
        <property type="protein sequence ID" value="CAZ81724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837533.1">
        <property type="nucleotide sequence ID" value="XM_002837487.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GB31"/>
    <dbReference type="STRING" id="656061.D5GB31"/>
    <dbReference type="GeneID" id="9187965"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005421001"/>
    <dbReference type="eggNOG" id="KOG2710">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004568_2_0_1"/>
    <dbReference type="InParanoid" id="D5GB31"/>
    <dbReference type="OMA" id="SHPDHEM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060237">
        <property type="term" value="P:regulation of fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04396">
        <property type="entry name" value="RhoGAP_fSAC7_BAG7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051025">
        <property type="entry name" value="RhoGAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15228:SF25">
        <property type="entry name" value="F-BAR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15228">
        <property type="entry name" value="SPERMATHECAL PHYSIOLOGY VARIANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="3">
        <location>
            <begin position="90"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="296"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="705"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="739"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="296"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="335"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="393"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="451"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="467"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="568"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="591"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="801"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="840"/>
            <end position="849"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ81724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="849" mass="91953" checksum="98929110F91DEC4C" modified="2010-06-15" version="1">MTTADGPSPGVHPGSPPTKASLASWWERFKKKPPPKEDGSAPISHCSLVSINRANYLQVLELTLILLVEHPTGIFSVPLHKSIQYANVAISLTDPNGEQFIYGYVPIVVAKCGVFLKEKATDVEGIFRLSGSAKRIKDLQTIFNSPDKYGKGLDWTGYTVHDAANVLRRYLNQLPEPIIPLEFYYRFREPLVTQPEGQEILSAIRSYQRLISELPPLNRQLLLYILDLLAVFASKAEQNLMNASNLAAIFQPGLISHPNHDMAPGEYKLSQEVLVFLITHQDHFLLGMRGSEDQNQASLVGPITPSSPRRSKTVVSRSPSNASAGADDVRKYGGVRRNVSVTSRRSSGSPVVSSVGVAGGGVNGSGLNRSNTLPSKRSPAVRPAAFAPGDVSPSPNSPHTLAPTSNITRSPSIGGQMRENVGPTSPDIALAEPKREASSTSPPRTEVPRQAPREPPRELPGESLESPQAQPQPVVTPTKERTFQNFFSLSPEGDRPGRPPNKLKKRRIPGSGGNSAESSTTSLSGVGGGPNPNGPAPTSHGALEPSKLLFPRPQSPPPRSPNIDIALPSSSQTNHASAGNPPSALMPAMSPTPSATSSVTSHSSTHSQSDTATNPASSPGKRKRRWRLSNPRFENPPVSSTPPNGGGSSGSLVERIRKASRSPPARQRSVSGETSHLVEEDNHAKAPKNIFGWLQKSIDERKYQFEREKEKEREKEKEKERQKAERQVALQMDQFIYPKSPPIQPLAPSSPLVQPPTPLFQYQADMPSPMSPIQEKGASPPAVDGPTEQLRKPTLLTESKQNSEETIRDVKPKECPERVSIPGESIEPEGRAPCQPVEAKVLEQPEPKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBB4</accession>
    <name>D5GBB4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00000402001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430090">
        <property type="protein sequence ID" value="CAZ81807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837616.1">
        <property type="nucleotide sequence ID" value="XM_002837570.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBB4"/>
    <dbReference type="STRING" id="656061.D5GBB4"/>
    <dbReference type="GeneID" id="9181518"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000402001"/>
    <dbReference type="eggNOG" id="KOG3986">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012287_0_0_1"/>
    <dbReference type="InParanoid" id="D5GBB4"/>
    <dbReference type="OMA" id="TYSKAVH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000164">
        <property type="term" value="C:protein phosphatase type 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001069">
        <property type="term" value="F:glycogen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008157">
        <property type="term" value="F:protein phosphatase 1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005979">
        <property type="term" value="P:regulation of glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2440">
        <property type="entry name" value="Carbohydrate binding type-21 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005036">
        <property type="entry name" value="CBM21_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038175">
        <property type="entry name" value="CBM21_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050782">
        <property type="entry name" value="PP1_regulatory_subunit_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12307:SF36">
        <property type="entry name" value="GLYCOGEN-BINDING SUBUNIT 76A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12307">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03370">
        <property type="entry name" value="CBM_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51159">
        <property type="entry name" value="CBM21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBM21" evidence="2">
        <location>
            <begin position="312"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="218"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="265"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="431"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="542"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="679"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="9"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="225"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="442"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="570"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="586"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="689"/>
            <end position="703"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ81807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="737" mass="80207" checksum="87D632483F7E9348" modified="2010-06-15" version="1">MPYTPPSQSPATSPAASRSSSMSLHDARPPATTMSNTVMKSSNPPRSATYLYKNRRTPTTSPSINNQYLPPRPLVQHRHSDSGGSIHRPPVPVDNGMSMPTGAIISPPDSSQNSSDEEEAAKGNGNTAVRGRRRDLGSLELQLKEAIKNIPQRREPSPDSTNPKSPSPTDPESKGRRLHSRSASETMLAYAINGGSGSGSEDDDEGYRIAPPMVRKKSGEVVKSSLKTPNRSRPCSMPSTPTFPKAVHFDSHIEHVRHFLHSEKPSAVSAGSSPVESYDGETEFPFSGEDSFRSREPPFEWEISLPNFPKDSDSRKHMPVRIERVVLSTDKKNLIGTVAVSNIAFQKSVVVRFTLDYWETVSEVSAEYNNDVRKKQREEGVDRFSFNIKLVDQANLEKKLLFFCVRYTSGGQEFWDNNNGFNFQVEFKKKAKPQNGKNGGARPLPRSSKPSSRPRILPNNFDDELESFEDAGIFEKLKNAELPEPKSSNLTDTTESLARRANPSGNAFGNRYDFNASFAAIQAAEASPGKKMRRKTINFDDVDSPYFKSASPPNPAPEEPKAQVVPALKPSLPTSSLPRSSRSVVKDTTSLDSPPASGSGSPTRGYPPAPFAGGKPSIESSSYRELLDNYCFFGSSKASPQLSQGAEIPDKMHNPTNLNTALYSGAVFATDVGPGSPLPETAISPRYVSSPSHNSPPSSLKSPGNASPIPANFAGYHHHRRASGAFKFSAQTPTAIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>D5GC34</accession>
    <name>D5GC34_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00000545001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TTC36 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430099">
        <property type="protein sequence ID" value="CAZ82077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837886.1">
        <property type="nucleotide sequence ID" value="XM_002837840.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GC34"/>
    <dbReference type="STRING" id="656061.D5GC34"/>
    <dbReference type="GeneID" id="9181346"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000545001"/>
    <dbReference type="eggNOG" id="KOG4555">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074601_0_0_1"/>
    <dbReference type="InParanoid" id="D5GC34"/>
    <dbReference type="OMA" id="CNQMLCE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006570">
        <property type="term" value="P:tyrosine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038906">
        <property type="entry name" value="TTC36"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21405">
        <property type="entry name" value="CDNA SEQUENCE BC021608"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21405:SF0">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="203" mass="21980" checksum="6BBDB6907A2349C4" modified="2010-06-15" version="1">MSALTINDSTVLTQLFDPESAPSSASPSIDASLPTDPYTPFDLLQTLKQTELKAIKLAESSPTSLPESRKILEELTTAHPTYASAHNNLAQVLRMLSVPATEILPHLNEAIKLSSPSTPTSSLSPSQAKILSQAYTQRAAIYYSMFKQGGSEDMEAAASRDFFEGGRYGNGIAREMAVRTNPYARLCGAIVKESMKNEYGECL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>D5GC99</accession>
    <name>D5GC99_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ribonuclease Z</fullName>
            <ecNumber evidence="4">3.1.26.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00000622001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule.</text>
            <dbReference type="EC" id="3.1.26.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase Z family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.11" evidence="4"/>
    <dbReference type="EMBL" id="FN430107">
        <property type="protein sequence ID" value="CAZ82142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837951.1">
        <property type="nucleotide sequence ID" value="XM_002837905.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GC99"/>
    <dbReference type="STRING" id="656061.D5GC99"/>
    <dbReference type="GeneID" id="9184821"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000622001"/>
    <dbReference type="eggNOG" id="KOG2121">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_947293_0_0_1"/>
    <dbReference type="InParanoid" id="D5GC99"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042781">
        <property type="term" value="F:3'-tRNA processing endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990180">
        <property type="term" value="P:mitochondrial tRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047151">
        <property type="entry name" value="RNZ2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12553">
        <property type="entry name" value="ZINC PHOSPHODIESTERASE ELAC PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12553:SF49">
        <property type="entry name" value="ZINC PHOSPHODIESTERASE ELAC PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="249"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="249"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="285"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ82142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="294" mass="32918" checksum="6F4FD5B8DB396027" modified="2010-06-15" version="1">MKFQCTVVTMPTSDLPEHAYSTLMANATFSVISEKEPSGPVLKAWFNEVYPLANSEANQLMNVVASWKFLVWLREYADVEDCEDLLRKNLNKPNHLTAHLPEVLDSLSLASIQTSRAVHCLSSFTTSWTWSNGFKIAYSRDTRPTKGFVESGQNSTVLHEVTFDDELLPEAITKKHSTTSEAINAGKEMGAKNFILTHFSQRYPRLPILSAVDNGPEVLIAFDLCRVRLGDVKRFGAFIPALTELYEEADEEKIEGEEEVEEEPVVEEKKGKVGQARGYKPGGNKSEKKSDKRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCH5</accession>
    <name>D5GCH5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00005894001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430109">
        <property type="protein sequence ID" value="CAZ82218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838027.1">
        <property type="nucleotide sequence ID" value="XM_002837981.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCH5"/>
    <dbReference type="FunCoup" id="D5GCH5">
        <property type="interactions" value="373"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GCH5"/>
    <dbReference type="GeneID" id="9186400"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005894001"/>
    <dbReference type="eggNOG" id="KOG1430">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_6_8_1"/>
    <dbReference type="InParanoid" id="D5GCH5"/>
    <dbReference type="OMA" id="STAHWFD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102175">
        <property type="term" value="F:3-beta-hydroxysteroid dehydrogenase (NAD+)/C4-decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002225">
        <property type="entry name" value="3Beta_OHSteriod_DH/Estase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43000">
        <property type="entry name" value="DTDP-D-GLUCOSE 4,6-DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01073">
        <property type="entry name" value="3Beta_HSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="277"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="3-beta hydroxysteroid dehydrogenase/isomerase" evidence="2">
        <location>
            <begin position="13"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ82218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="359" mass="39685" checksum="B9527158C1F8B850" modified="2010-06-15" version="1">MTSRKRQHLNNALIVGGCGFLGQHIVTLLQEHHPECDIFVMDLRLSNTLPGVTYLEGDVTSRESVDSILNKVKPEVVIDTVSPVHGLGEAIYYKVNVDGTKVLLEASSDAGVKVFVFTSSASVVFDGASDLINVNESAPIASPAMDPYTETKAIGEKMVLEANRKGGMFTIALRLSGLFGPGDRQLIPGMLGVLARGQTKFQIGNNENLFDFTYIVNAAWAHILATEKLIALSPHTPKTLETPDGETYFITNGEPCYFWDFPRTIWAIRGHIAPFHIVMPAAVGIAMGGAAELFAWLLNREPGLSRFRVRFSCWNRYFDIRKAKQMLGYQPLVKLHDGLVETLKWFNEQEAMEVAKKGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCH7</accession>
    <name>D5GCH7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00005896001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430109">
        <property type="protein sequence ID" value="CAZ82220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838029.1">
        <property type="nucleotide sequence ID" value="XM_002837983.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCH7"/>
    <dbReference type="GeneID" id="9186402"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005896001"/>
    <dbReference type="HOGENOM" id="CLU_1866593_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCH7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="137" mass="14554" checksum="563A9D5C0FF6C0A8" modified="2010-06-15" version="1">MPLPEASPLNIYLGSNALISLSTAYFYLTSPAPSIVKLGGKPSPTSTVLTQLLASGEALISYICLEGLFSSSSEMRAMVVRGVGIYTLIHMGTFWWGHKKHVKNPKGAGTFVGGLVLGAGVGVWWGVLRREGLPAFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCR2</accession>
    <name>D5GCR2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00005978001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430120">
        <property type="protein sequence ID" value="CAZ82305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838114.1">
        <property type="nucleotide sequence ID" value="XM_002838068.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCR2"/>
    <dbReference type="STRING" id="656061.D5GCR2"/>
    <dbReference type="GeneID" id="9187924"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005978001"/>
    <dbReference type="HOGENOM" id="CLU_1062423_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCR2"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072659" evidence="2">
        <location>
            <begin position="25"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ82305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="262" mass="30058" checksum="F45F0D10C5F7EFB3" modified="2010-06-15" version="1">MLAVTFGTIILLMNLAFLRRNLEALKTWAQRSIRLRMAGSLDAKVSSKEPTYQEKRPGWKFWKERAQSLREAEKRSALLTDNAIHDSGSDWWYWYFMAIFVIIVVPVQELTFIIRALRLQKIKKAGPLKKFVRVPWAPIWILQLVLVYMILLAGYAFLSLIGLAHRTAVWLWTGDDITEGERAPPEGIEKNLMSQQEGYEAIWKSEPDSTKEHRGLVGWLMKPAKAMQLLIVVEALRSKEVKEFDAEEQGTTVEAMEAPPST</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>D5GD51</accession>
    <name>D5GD51_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00000976001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430140">
        <property type="protein sequence ID" value="CAZ82444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838253.1">
        <property type="nucleotide sequence ID" value="XM_002838207.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GD51"/>
    <dbReference type="STRING" id="656061.D5GD51"/>
    <dbReference type="GeneID" id="9183908"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000976001"/>
    <dbReference type="eggNOG" id="ENOG502S0T9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_268750_0_0_1"/>
    <dbReference type="InParanoid" id="D5GD51"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000637">
        <property type="entry name" value="HMGI/Y_DNA-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018866">
        <property type="entry name" value="Znf-4CXXC_R1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10497">
        <property type="entry name" value="zf-4CXXC_R1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00354">
        <property type="entry name" value="HMGI_Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="JmjC" evidence="6">
        <location>
            <begin position="143"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="639"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="749"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="918"/>
            <end position="1220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="676"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="918"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="956"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="1038"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1070"/>
            <end position="1090"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1136"/>
            <end position="1146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1167"/>
            <end position="1181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ82444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1220" mass="136847" checksum="BD4DCDBAEE04AAB6" modified="2010-06-15" version="1">MSAVGIPLFTPIPPGFDVKQLAEEPGSHFHLATRVDARTLTPNTAEQFDEIYRSQVLQIGEPLVIENWHLKSRWPGHIFKDEWLDNNLGRQRVQIRDLVKGSDDEWSLGWYLSKCETLASKFTPENYKSIKRQRLYLKDMDCPSVWMKNLGDILPRGTFYLDENADLMSALPEELQAVNMMCYVGHEGTFTPCHKEMCSSIGQNIMVSASENGRSIWFLVKPQDRAKLAVWFRSVLGQDLELENYFASIEDWKKAPVDVWIVEQKVGDFLIIPPASPHQVWNQGDLTIKAAWNRCTPESLKVCLSGELSMSKFACREEAYKVKTMVYMAILKIANELDRAPSNEDEGIVRDFLTLYPLFEAIMLDEAFSPELPIPAVEDVQAEYGITCSFCSGNIFNRFLSCPSCPIGDGDNADCYDICMDCYTLGRRCKCDTPPKWVQQYPWATLQNDHERFRQIFNRLIEDERFASILKRIGVGRSSRESLDHLTSVENVTRRSRAWVSQMLSDMYTRYPGQRRGARVGKDITKDEADNKRNCHVCKNPHAPIIYVECSDADCNKRYCYGYLWRAHSQNPYMILSSMNWRCPFCLKQCTCAACLKSGRNGRRARRNNPIWLGYNTRAVTDQRSEEWLTTFAQHNKKWATSGGDGDSASQKRKRSDGSGEDSDADADSNSSSGDNEVEVEDREGISDEDDVMDDDEGREWEGPGKHPSLLEFPSAVGVRGGDPSFISGKRSTNVNLLASQLAILARECGPSTGETDGKGEGIDTIGGRGRDQNNSLALFDNHSLENNTIASPLCPGLDNSALRSDNLSQLEMDPQAFRIAAGLLTVEDNMPASLAQQPSTVSPEALSLPLLLTGPARLEVERIVKSPTPEPIETKAGWVKKAKLARKRFANLQAGKNRRKSLIVKLKVTVEEWEETPVRSDVPPKKIVSEERKKEDEALIVQPTSPPQQHQGESPLEDDVEESAEDIPSDGPPSRRKAVPRDGAYVARNRTGNPPAPLMALSSVRSRRRSQSLQGSARNHSAHSSKHTPSLEQASTPAPPPLLSPPPPPPLTSLIPADWPFAIIITKKPERRKYPKTTTTRRPRGRPRKAQPTGRAREDGDFKLASWSKAMKAESTTLTKGSVRPRVASTAFAEGVEESSEDDEPSCYVERSPRPVSVDSGEEGDREGPRSERTAVEKGGARKQRKMEPKEEEVGEGRGGDYDEEDEYNRRWSRRRLFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDB7</accession>
    <name>D5GDB7_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">ATP-dependent RNA helicase DBP5</fullName>
            <ecNumber evidence="4">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">ATP-dependent RNA helicase dbp5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="ORF">GSTUM_00006139001</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27 28">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">ATP-dependent RNA helicase associated with the nuclear pore complex and essential for mRNA export from the nucleus. May participate in a terminal step of mRNA export through the removal of proteins that accompany mRNA through the nucleopore complex. May also be involved in early transcription.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="17">Associates with the nuclear pore complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the DEAD box helicase family. DDX19/DBP5 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="4"/>
    <dbReference type="EMBL" id="FN430142">
        <property type="protein sequence ID" value="CAZ82510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838319.1">
        <property type="nucleotide sequence ID" value="XM_002838273.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDB7"/>
    <dbReference type="FunCoup" id="D5GDB7">
        <property type="interactions" value="735"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GDB7"/>
    <dbReference type="GeneID" id="9183974"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006139001"/>
    <dbReference type="eggNOG" id="KOG0332">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_1_0_1"/>
    <dbReference type="InParanoid" id="D5GDB7"/>
    <dbReference type="OMA" id="IAAETRW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17963">
        <property type="entry name" value="DEADc_DDX19_DDX25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000849">
        <property type="entry name" value="ATP-dependent RNA helicase DBP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000318">
        <property type="entry name" value="ATP-dependent RNA helicase DDX19B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 22" id="KW-0347">Helicase</keyword>
    <keyword evidence="7 22" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="13" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="6 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="12" id="KW-0811">Translocation</keyword>
    <keyword evidence="9" id="KW-0813">Transport</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="26">
        <location>
            <begin position="69"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="24">
        <location>
            <begin position="102"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="25">
        <location>
            <begin position="280"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="23"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="21">
        <location>
            <begin position="69"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00037213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00038143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00038750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00039326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00039604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EMBL" id="CAZ82510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="465" mass="51304" checksum="5DA1B771EAB582A9" modified="2010-06-15" version="1">MAEQPASLESRIGKVPEVPVGAAVTTDKASTSIPPPAAGGSGLDEPQYEVAVKLADLQGDPNSPLYSVKKFEDLGLSKELLEGVYFMNFKKPSKIQERALPLLLSDPPTNMIGQSQSGTGKTAAFVLTMLTRVDMSVSNVQALCLAPSRELARQIMSVVQTMGQFTDIRTQFAIPNMVQRGQKIDAHIVVGTPGTVLDLIRRKQLPVQHLKVFVLDEADNMLDQQGLGDQCLRVKQNIPATAQIVLFSATFPDQVVQYANLFAPDANQITLKHEELTVDGIKQLYMDCDSEEDKYRILVELYHILTIGSSIIFVKKRETASEIQRRMEADGHKVAALHGAQEGVDRDRVIDDFRSGRAKVLITTNVLARGIDVATVSMVVNYDIPLDQNRRPDPQTYLHRIGRTGRFGRVGVSISFVHDKQSWSEMNEISNYFGVSMTRVPTNDIDEVEKIVNEVLKSKTAGATK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDC2</accession>
    <name>D5GDC2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00006145001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430142">
        <property type="protein sequence ID" value="CAZ82515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838324.1">
        <property type="nucleotide sequence ID" value="XM_002838278.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDC2"/>
    <dbReference type="GeneID" id="9183979"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006145001"/>
    <dbReference type="HOGENOM" id="CLU_2529109_0_0_1"/>
    <dbReference type="InParanoid" id="D5GDC2"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072708" evidence="1">
        <location>
            <begin position="20"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="84" mass="9564" checksum="94CEE5DAF07E9EC2" modified="2010-06-15" version="1">MHVLCFFFFFPLPFPPFAGVVNPLRHVFASPSLHLYPTFHFPGFPIIISSFPCPGKVSSLQSLYITRYNSPDREGEREFGRSVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDH9</accession>
    <name>D5GDH9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="16">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">GSTUM_00001029001</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family. ISWI subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430144">
        <property type="protein sequence ID" value="CAZ82572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838381.1">
        <property type="nucleotide sequence ID" value="XM_002838335.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDH9"/>
    <dbReference type="FunCoup" id="D5GDH9">
        <property type="interactions" value="1089"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GDH9"/>
    <dbReference type="GeneID" id="9188162"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001029001"/>
    <dbReference type="eggNOG" id="KOG0385">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000315_0_2_1"/>
    <dbReference type="InParanoid" id="D5GDH9"/>
    <dbReference type="OMA" id="VHDYQFF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140658">
        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034728">
        <property type="term" value="P:nucleosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17997">
        <property type="entry name" value="DEXHc_SMARCA1_SMARCA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000082">
        <property type="entry name" value="ISWI chromatin remodeling complex ATPase ISW1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000002">
        <property type="entry name" value="ISWI chromatin-remodeling complex ATPase CHR11 isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000234">
        <property type="entry name" value="ISWI chromatin-remodeling complex ATPase ISW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.30:FF:000003">
        <property type="entry name" value="ISWI chromatin-remodeling complex ATPase ISW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1190">
        <property type="entry name" value="iswi atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.30">
        <property type="entry name" value="ISWI, HAND domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044754">
        <property type="entry name" value="Isw1/2_DEXHc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015194">
        <property type="entry name" value="ISWI_HAND-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036306">
        <property type="entry name" value="ISWI_HAND-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015195">
        <property type="entry name" value="SLIDE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623:SF49">
        <property type="entry name" value="SWI_SNF-RELATED MATRIX-ASSOCIATED ACTIN-DEPENDENT REGULATOR OF CHROMATIN SUBFAMILY A MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09110">
        <property type="entry name" value="HAND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09111">
        <property type="entry name" value="SLIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101224">
        <property type="entry name" value="HAND domain of the nucleosome remodeling ATPase ISWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="147"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="428"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="15">
        <location>
            <begin position="794"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="75"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="750"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="975"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="75"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1006"/>
            <end position="1017"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1025"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1043"/>
            <end position="1061"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAZ82572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1069" mass="122177" checksum="8FD8C7317CB1234C" modified="2010-06-15" version="1">MAATKTMRTSRTEAAMMTMTPQLKTRLMDKKAFGKKNDTLNKTKENDSMKRIRYLLGLTDLFRHFMSNNPDYQRVKAEEEEKSRQQAKNRKGSTDNRRRRTEKEEDEELLQDEIVDGGTAAIFTESPKFIKGGQMRDYQIAGLNWLISLHENGISGILADEMGLGKTLQTISFLGYLRYVAGIKGPHLITVPKSTLDNWKREFEKWTPDVKVLVLQGTKEERQKLIQELVLTDGFDCLVTSYEMILREKTHLKKFAWEYIIVDEAHRIKNEESALAQIIRLFNSRNRLLITGTPLQNNLHELWALLNFLLPDVFGDSAAFDEWFENQGGDQDVVVQQLHKVLRPFLLRRVKSDVEKSLLPKKEVNILEKDIDAVNGAGGKRESKTRLLNIVMQLRKCCNHPYLFEGAEPGPPYTTDEHIIDNSGKMVMLDKLLKRMKAQKSRVLIFSQMSRQLDILEDYCVFREYPYCRIDGSTAHEDRITAIDEYNKPGSEKFIFLLTTRAGGLGINLTTADIVVLYDSDWNPQADLQAMDRAHRIGQTKQVMVFRFVTENAIEEKVLERAAQKLRLDQLVIQQGRSQQQAKAAANKDELLSMIQHGADLVFKGGKEKEAGISQNMTEDDIDAILKRGEERTAELNARYAKLGLDDLQKFTSDSAYEWNGENFAAIKKPSIGANWINPAKRERKEQSYGIDNYYRTVLTSGNKAADTKPKAPRAPKQVNVLEHQFYPPRLVELQDRETAYFRKQQGYKVPLPDGEADDLEERQAARDQEQEAIDNATPLTEEEQAEKAHLAEQGFSSWSRKDFGTFVTLSAKYGRKNFAMIAQEMESKEEKEVRKYAKVFWERYKEMPNWEKCINTIETGEEKSAKISQQKKMLRKKIEMYRVPLQQLKLSYSVSTTNKKVYTEEEDRFLLVQLDKFGLDAENLHEKIRDEIRESPTFRFDWFLLSRTSLEISRRCTTLISTVAREFEENGNGVAAAKATAAKKGRDREEEEEEEGPPAKKGKANSGTAKETNGKTAKQPPKNKQVEQTKKGGDSKATSNATSRASSVDSKASAATSVSKSKSRSKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDP6</accession>
    <name>D5GDP6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00006216001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430153">
        <property type="protein sequence ID" value="CAZ82639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838448.1">
        <property type="nucleotide sequence ID" value="XM_002838402.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDP6"/>
    <dbReference type="FunCoup" id="D5GDP6">
        <property type="interactions" value="563"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GDP6"/>
    <dbReference type="GeneID" id="9181644"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006216001"/>
    <dbReference type="eggNOG" id="KOG3275">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056776_3_0_1"/>
    <dbReference type="InParanoid" id="D5GDP6"/>
    <dbReference type="OMA" id="YRVVMNC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043530">
        <property type="term" value="F:adenosine 5'-monophosphoramidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01277">
        <property type="entry name" value="HINT_subgroup"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039384">
        <property type="entry name" value="HINT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019808">
        <property type="entry name" value="Histidine_triad_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001310">
        <property type="entry name" value="Histidine_triad_HIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46648:SF1">
        <property type="entry name" value="ADENOSINE 5'-MONOPHOSPHORAMIDASE HNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46648">
        <property type="entry name" value="HIT FAMILY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01230">
        <property type="entry name" value="HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00332">
        <property type="entry name" value="HISTRIAD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00892">
        <property type="entry name" value="HIT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HIT" evidence="4">
        <location>
            <begin position="7"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Histidine triad motif" evidence="2 3">
        <location>
            <begin position="95"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-AMP-histidine intermediate" evidence="1">
        <location>
            <position position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR601310-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR601310-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ82639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="135" mass="14714" checksum="B657262838351196" modified="2010-06-15" version="1">MSAAACIFCKIIRGEIPSFKLLETDKILAFLDINPLSRGHALLIPKTHGEKLHDVPDAELSELLPAAKRLALAAGAVEYNILQNNGRGAHQLVDHVHVHMIPKPNAKEGLGIEWPAQATDMDGLKALFEELKAKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDQ8</accession>
    <name>D5GDQ8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">chitinase</fullName>
            <ecNumber evidence="3">3.2.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00006229001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.14"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 18 family. Chitinase class V subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.14" evidence="3"/>
    <dbReference type="EMBL" id="FN430153">
        <property type="protein sequence ID" value="CAZ82651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838460.1">
        <property type="nucleotide sequence ID" value="XM_002838414.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDQ8"/>
    <dbReference type="STRING" id="656061.D5GDQ8"/>
    <dbReference type="GeneID" id="9181656"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006229001"/>
    <dbReference type="eggNOG" id="KOG2806">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002833_1_0_1"/>
    <dbReference type="InParanoid" id="D5GDQ8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008843">
        <property type="term" value="F:endochitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.10:FF:000005">
        <property type="entry name" value="Endochitinase B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011583">
        <property type="entry name" value="Chitinase_II/V-like_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029070">
        <property type="entry name" value="Chitinase_insertion_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050314">
        <property type="entry name" value="Glycosyl_Hydrlase_18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177">
        <property type="entry name" value="CHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177:SF317">
        <property type="entry name" value="CHITINASE 12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00636">
        <property type="entry name" value="Glyco_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54556">
        <property type="entry name" value="Chitinase insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0146">Chitin degradation</keyword>
    <keyword evidence="7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GH18" evidence="9">
        <location>
            <begin position="4"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ82651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="375" mass="41041" checksum="8BF57E30749BFDCE" modified="2010-06-15" version="1">MFGKKTVAYFVNWGIYGRNYQPQDIPASHLTHILYSFANVRPESGEVYLSDPYSDLEKHYPTDSWNDVGTNVYGCGGHTPPTLRRQQLRLLAGVHSPGLPFNSSGTWDLMVSILIGMPFHATQAQNFVSLLAECRTQLDAYGNQYAPGQKLLLTIAAPCGATHYTKLLISQMNQYLDFWNLMAYDFAGSWDSTAGHQANLYHSASNPESTPFSANDAVNAYIAGGVPCNKIVLGMPLYGRSFQATTGPGASFTGIGNGSWENGVWDYKALPKAGAAECADNSTVASWSYDSAAREMISYDTPTIAQTKSSYILEKGLGGGMWWELSSDKPIGDPRSLIKTTVDRFGGTGSLETTNNILTYPGSKYDNMRNQFRSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDT4</accession>
    <name>D5GDT4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00006262001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430153">
        <property type="protein sequence ID" value="CAZ82677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838486.1">
        <property type="nucleotide sequence ID" value="XM_002838440.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDT4"/>
    <dbReference type="GeneID" id="9184472"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006262001"/>
    <dbReference type="HOGENOM" id="CLU_3320331_0_0_1"/>
    <dbReference type="InParanoid" id="D5GDT4"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="39" mass="4094" checksum="FD29A14A75934C92" modified="2010-06-15" version="1">MDRRFGFSNSTSFGSLSSLESLGEKSPGVGELPFAQSSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDV6</accession>
    <name>D5GDV6_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">argininosuccinate lyase</fullName>
            <ecNumber evidence="4">4.3.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Arginosuccinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00006295001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-(N(omega)-L-arginino)succinate = fumarate + L-arginine</text>
            <dbReference type="Rhea" id="RHEA:24020"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:57472"/>
            <dbReference type="EC" id="4.3.2.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the lyase 1 family. Argininosuccinate lyase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.1" evidence="4"/>
    <dbReference type="EMBL" id="FN430153">
        <property type="protein sequence ID" value="CAZ82699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838508.1">
        <property type="nucleotide sequence ID" value="XM_002838462.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDV6"/>
    <dbReference type="FunCoup" id="D5GDV6">
        <property type="interactions" value="1006"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GDV6"/>
    <dbReference type="GeneID" id="9184494"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006295001"/>
    <dbReference type="eggNOG" id="KOG1316">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027272_2_1_1"/>
    <dbReference type="InParanoid" id="D5GDV6"/>
    <dbReference type="OMA" id="DFAIEFC"/>
    <dbReference type="UniPathway" id="UPA00068"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004056">
        <property type="term" value="F:argininosuccinate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:L-arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01359">
        <property type="entry name" value="Argininosuccinate_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.275.10:FF:000002">
        <property type="entry name" value="Argininosuccinate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.40.30:FF:000001">
        <property type="entry name" value="Argininosuccinate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.200.10:FF:000025">
        <property type="entry name" value="Argininosuccinate lyase chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.40.30">
        <property type="entry name" value="Fumarase/aspartase (C-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.200.10">
        <property type="entry name" value="Fumarase/aspartase (Central domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="entry name" value="Fumarase/aspartase (N-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00006">
        <property type="entry name" value="Arg_succ_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029419">
        <property type="entry name" value="Arg_succ_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009049">
        <property type="entry name" value="Argininosuccinate_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00838">
        <property type="entry name" value="argH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43814">
        <property type="entry name" value="ARGININOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43814:SF1">
        <property type="entry name" value="ARGININOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14698">
        <property type="entry name" value="ASL_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00145">
        <property type="entry name" value="ARGSUCLYASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="L-aspartase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumarate lyase N-terminal" evidence="6">
        <location>
            <begin position="1"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="Argininosuccinate lyase C-terminal" evidence="7">
        <location>
            <begin position="350"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00032749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ82699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="444" mass="49709" checksum="608B8B44A92F159E" modified="2010-06-15" version="1">MTTYNESIHYDKRMYLADILGSKVYATSLHQRNIITAHELSELHRGLDLVHAEWANDTFAISPGVDEDIHTANERRLGELIGKGIAGKLHTGRSRNDQVATDTRIWLRDTLDEIESALVDLLRAHLGRAEADLDAIMPGYTHLQRAQPIRWSHWLMSHTYYFTDDLTRLRQLRIRVNRCPLGSGALAGNPFAVDRAFLATELGFDSVIGNSLVGVADRDFVAETMMWGSLFMSHVSRWAEDLILYSTQEFGFVKLADAYSTGSSLMPQKKNPDSLELLRGKSGRVFGQMVGFMYTLKALPSTYNKDLQEDKEPLFDTTRTLLDSTRIATGVLSTLSINREKMLAALTMDMLATDLADYLVRKGVPFRETHHISGAAVSLAENEGLSGIDKLGLDKLRTLSPKFDEDVMQVFNFEHSVEMRSAVGGTARESVLRQIGEVKALLRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEN6</accession>
    <name>D5GEN6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00001316001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430180">
        <property type="protein sequence ID" value="CAZ82979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838788.1">
        <property type="nucleotide sequence ID" value="XM_002838742.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEN6"/>
    <dbReference type="STRING" id="656061.D5GEN6"/>
    <dbReference type="GeneID" id="9184890"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001316001"/>
    <dbReference type="eggNOG" id="KOG0817">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043304_1_0_1"/>
    <dbReference type="InParanoid" id="D5GEN6"/>
    <dbReference type="OMA" id="WMRRKKD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000062">
        <property type="term" value="F:fatty-acyl-CoA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000582">
        <property type="entry name" value="Acyl-CoA-binding_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035984">
        <property type="entry name" value="Acyl-CoA-binding_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23310">
        <property type="entry name" value="ACYL-COA-BINDING PROTEIN, ACBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23310:SF133">
        <property type="entry name" value="COA BINDING PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G12300)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00887">
        <property type="entry name" value="ACBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47027">
        <property type="entry name" value="Acyl-CoA binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51228">
        <property type="entry name" value="ACB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="228"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="ACB" evidence="4">
        <location>
            <begin position="5"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="192"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ82979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="292" mass="33302" checksum="8588067C7E80EDFF" modified="2010-06-15" version="1">MSDSVDRVFAHALQTVRKIPKTGSARPPPEDRLKLYGLYKQSMEGNVNGVMERPSGYGNESKAERDKWDAWNSQAGLSKTEAKRQYITTLIETMHKYSSNTPEARELVSELEFVWEQIKSNVSSLSTPSSSTSPFAQHSSALLPMPSGSYPDFGGDSQHHLGLVSPNPFGEEEFDDEDTGMDTDRRSNRKWRRRVEQALAKMATEVAALREQLETARARERRGRLRNWVLWTIYVVGRHLVIEAVFWGLVFLWMRRKGDRVAQDALRLVIRFVRERLKFGSLRGKKGVKTAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GF32</accession>
    <name>D5GF32_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00006723001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430197">
        <property type="protein sequence ID" value="CAZ83125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838934.1">
        <property type="nucleotide sequence ID" value="XM_002838888.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GF32"/>
    <dbReference type="FunCoup" id="D5GF32">
        <property type="interactions" value="20"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GF32"/>
    <dbReference type="GeneID" id="9187986"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006723001"/>
    <dbReference type="eggNOG" id="KOG4711">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007711_0_0_1"/>
    <dbReference type="InParanoid" id="D5GF32"/>
    <dbReference type="OMA" id="VTWPAFV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018823">
        <property type="entry name" value="ArAE_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018820">
        <property type="entry name" value="BRE4-related_DUF2421"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023244">
        <property type="entry name" value="Brefeldin_A-sensitivity_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049453">
        <property type="entry name" value="Memb_transporter_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37994">
        <property type="entry name" value="ARAE_2_N DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37994:SF4">
        <property type="entry name" value="ER TRANSPORTER 6TM N-TERMINAL DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10337">
        <property type="entry name" value="ArAE_2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10334">
        <property type="entry name" value="BRE4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13515">
        <property type="entry name" value="FUSC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02047">
        <property type="entry name" value="BREFELDNASP4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82866">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="27"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="461"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="492"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="540"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="domain" description="Putative ER transporter 6TM N-terminal" evidence="8">
        <location>
            <begin position="4"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Integral membrane bound transporter" evidence="9">
        <location>
            <begin position="486"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2421" evidence="7">
        <location>
            <begin position="661"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="408"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="409"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="426"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF10334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF10337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ83125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="944" mass="106197" checksum="8D89A5F6B91C768F" modified="2010-06-15" version="1">MMMIRATFLTISLFVHGYVRSSTPRLFILVLLMVIPVVIGLTSPIYEMSTVFPRQFFFPILTASAIVLLVNVAIFPEFGSTYLGLTTIEALQKTLKVQKDASQLFLTYRNRQTSDINGNSAGKKPSLSDLTEAKAELRKKVAECKGALTECSFELAFSVLAPWELKPIASKGVKKLVANTLSLVGACESKYALLGDGGVTHNQAAENKHSGKMRSVEGANIDQVRPRKEIESGDQQLLRYLLERVEEPLSGLQATIDRCVDIINTCVACTFDVPTLPSLKQGEGTPRRPKGILVQELDVHIDWLENEIEEFGRRSTEALEGAALKETDNVEMIDGEIDVMPRDEIFLISSFMLNLRQAASHTLGMLKHSRKLVELHKDRKETRSIWFPKIKLRKWLFSGAEEPESLPAAKRDAYLKEGGDPDEDEMTSRETVRERKDQGREKTVRERVADFLEWAGDSDDVLYAFKLTLGVMLVTWPAFVSGWQMWFYYNRGLWVGLIFILVFENAVGPTIWIFALRAVGTLIGSVWGYAAYEARNGNEYVIAAMILVGMIPSYYVQLGTRYMKAGMICTISMCVVALSTHLQTVPGSSQENFTKRSVTMLIGGVAATLVQMIVLPAKARVRLKESLASAIVQITKMETCIAYGVDDTRNIISTPRLFRKFERSAKKAEAALTSAETFLGFTKQEPRLKGSFDTQEVVYKEIIFVLRQIVDKMENMLQMRKAYGSAVLEQYNSLVYGYRRNVAASITLMLFACHEALTTKLPLPQFLPSARLAHLRMVVRVRQILLEEDERQEREEPNERLDASAGARRRALKLKFLSWNASAAALEECIEYVEELVNLLKMLVGSNEFRSGLLHRPTYREYVEKMRQQRMGTGGRGVEGDKKSSTIASSLAGRLAKIRGEEEDASVEIDIPNPLQRIHSRRVAVKTGRQKTRTVEAVEEEDRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>D5GF51</accession>
    <name>D5GF51_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00001858001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430207">
        <property type="protein sequence ID" value="CAZ83144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838953.1">
        <property type="nucleotide sequence ID" value="XM_002838907.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GF51"/>
    <dbReference type="STRING" id="656061.D5GF51"/>
    <dbReference type="GeneID" id="9188015"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001858001"/>
    <dbReference type="eggNOG" id="KOG1343">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001880_0_0_1"/>
    <dbReference type="InParanoid" id="D5GF51"/>
    <dbReference type="OMA" id="VHIAIAH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004407">
        <property type="term" value="F:histone deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053244">
        <property type="entry name" value="HDAC_HD_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47558">
        <property type="entry name" value="HISTONE DEACETYLASE HOS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47558:SF1">
        <property type="entry name" value="HISTONE DEACETYLASE HOS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="2">
        <location>
            <begin position="110"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="557"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="709"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="614"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="716"/>
            <end position="734"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ83144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="863" mass="93546" checksum="7F11431DDF867717" modified="2010-06-15" version="1">MSAPTAVIFHDTSYRHRYSRPNTSLQDLATIVERPERIPAATLGVAAAQTRVGRSRLSIRKSERMGSLLDLEVMLVHCHAAPVRGAKSSWPEDLAAMCGAAAEKLKKGECEVPKGYHQGDLYLCEESKEDLEGCLGALYDGVDLVFGGDERNGTISEDDQKRRGVRRAFVCIRPPGKLISSQSQAHTNCQSIGHHCAETQPSGFCWLNNVHIAIAHAARAHGLSHAVILDFDLHHGDGSQAIAWTLNELAESTHNTSKRTSGIQVPNIGYFSLHDINSYPCEYGDIDKIKNASLNLHAHGQCMQNVHLAPYSTEGEFWTLYNDKYSSLLTKAREFLSVAAVSKPKKSREFKAGVFLSAGFDASEHEGAGMQRHAVNVPTSFFARFTSDAVALAEEYCHGRVLSVLEGGYSDRALTTGVFAHMAGLACAPPEAASTYVSPSLGLTPLHKPLENSGLDRGWDAEWWGMERVLELEGHFAKMTAKKPNVERASTSYLSPTAASVAKKNDVPRRVMSNGYLDGNNNNGPPPPPPVIPWEVAAFDLSRMIVPEFRESVEIPPIEKPTKRSARHSVIGVVDPDGTRMTLRDRKPKPAPDPSPDIDRGRRRMTTTGILPDSAASSRAPSRAVSRAPSRAPSKAPSRPPSRAQRVGAPSRGVTPIPPSRGTTPAPGGRQIGSTSGGGVTVAGPRKAVVKKPSVIGTDVSKRVVDQHPVTAVQKTSSTISSASSVASSSPTGSQEPMKTGCDDDVDSLTKGMFKVKLTYKNRDKDRAEQLRILEQEKERTERALETEKRRLATNHALKRKEGGVGRMKEAFERNLTGTTAAGSHAEKETKMVDNVEIPVACSPMDQTFHGGDIKFADPWQGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFD2</accession>
    <name>D5GFD2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00006838001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430208">
        <property type="protein sequence ID" value="CAZ83225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839034.1">
        <property type="nucleotide sequence ID" value="XM_002838988.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFD2"/>
    <dbReference type="GeneID" id="9188081"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006838001"/>
    <dbReference type="HOGENOM" id="CLU_2308121_0_0_1"/>
    <dbReference type="InParanoid" id="D5GFD2"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="100" mass="10741" checksum="8B4443C26980778A" modified="2010-06-15" version="1">MPVLARSATCRGTLADMSTPEIIASPIPLRRSSRTEYPDSLDTSSSPALIDRMSAPKSSTRCFALSTVFPCHPSVDVDESSKTRPAASERGKSRSEILIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFI4</accession>
    <name>D5GFI4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00006914001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the metallo-beta-lactamase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430208">
        <property type="protein sequence ID" value="CAZ83277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839086.1">
        <property type="nucleotide sequence ID" value="XM_002839040.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFI4"/>
    <dbReference type="STRING" id="656061.D5GFI4"/>
    <dbReference type="GeneID" id="9188225"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006914001"/>
    <dbReference type="eggNOG" id="ENOG502SKHF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030571_1_1_1"/>
    <dbReference type="InParanoid" id="D5GFI4"/>
    <dbReference type="OMA" id="NGWRESA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07730">
        <property type="entry name" value="metallo-hydrolase-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051013">
        <property type="entry name" value="MBL_superfamily_lactonases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42978:SF2">
        <property type="entry name" value="102 KBASES UNSTABLE REGION: FROM 1 TO 119443"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42978">
        <property type="entry name" value="QUORUM-QUENCHING LACTONASE YTNP-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="6">
        <location>
            <begin position="37"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ83277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="284" mass="31479" checksum="0B0BF0D54FC7BA1C" modified="2010-06-15" version="1">MATLVVHTLEAGRAFLPERLLFNDTTPTNADRAYEFPAHVFHLHHPGKNLRMLWDAGMRSDISTYAPAMQAAFEAYLHPYVREDAPTGLSRNGVTPESIDYIFCSHAHFDHTGDADKFPKAKIVYGAGTQSHVRPAYPIDQGSRYLESLFPEGRTLELAKGDFKPIEGFAPWDTACDFFGDGSLLLIDAPGHMPGHMIALARVEGGYLLLGGDSCHHEAHLKEYVPGGRMGFGMHEDADGVRENISKIVEFAKARGDVRLCLAHVGTYKDWERVVLKEGEGMRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GG02</accession>
    <name>D5GG02_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007134001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430231">
        <property type="protein sequence ID" value="CAZ83445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839254.1">
        <property type="nucleotide sequence ID" value="XM_002839208.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GG02"/>
    <dbReference type="STRING" id="656061.D5GG02"/>
    <dbReference type="GeneID" id="9181790"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007134001"/>
    <dbReference type="eggNOG" id="KOG1198">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_3_1_1"/>
    <dbReference type="InParanoid" id="D5GG02"/>
    <dbReference type="OMA" id="CDIRGVF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08241">
        <property type="entry name" value="QOR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002364">
        <property type="entry name" value="Quin_OxRdtase/zeta-crystal_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051397">
        <property type="entry name" value="Zn-ADH-like_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43677:SF4">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43677">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE/REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01162">
        <property type="entry name" value="QOR_ZETA_CRYSTAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="1">
        <location>
            <begin position="6"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="329" mass="35249" checksum="27F7EC665D443004" modified="2010-06-15" version="1">MKGIQGPQDLKVIDLPDPTPAPGEYLIQVRASATNFFDLLQIRGKYQHQPPFPWISGLEFAGTVLAAGASAKKFKVGDRVFGAKQGAYATKICVKEPVLHPVPESWGFNDAAGLYVTAPTSYQGLVGRAGVKKGDWVLVHAGAGGVGLAAIQIAKYFGAKVIATASTDGKLEVCKRFGADYAIDYSKGTWPKSVLEITGGKGVDIVYDPVGLVEQSLKCIAWNGRILIIGFAGGKIEKVAMNRVLLKNVSLVGLHWGRYTEIQNEKGTVEDVWSGILGMIKDGGFRSTVYRGEEFVGLESVPRALKALGARETWGKVVVSVPKEGESKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>D5GG72</accession>
    <name>D5GG72_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007242001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430242">
        <property type="protein sequence ID" value="CAZ83515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839324.1">
        <property type="nucleotide sequence ID" value="XM_002839278.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GG72"/>
    <dbReference type="STRING" id="656061.D5GG72"/>
    <dbReference type="GeneID" id="9184577"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007242001"/>
    <dbReference type="HOGENOM" id="CLU_1929126_0_0_1"/>
    <dbReference type="InParanoid" id="D5GG72"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="131" mass="13220" checksum="0D01D7DF8DE3530B" modified="2010-06-15" version="1">MMAKRPIKPAVEQPNPSATTTSVSTSEDQQQQAQQQRGTGGGGGSGGVGAGGSGAGGQGAVPADVDTLSPTTPVSNKKRRALKQPVAPGTSPLAGPSAGAKRTRTNTPWTAAEEQRLKSMRDAGNSWKALK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGC2</accession>
    <name>D5GGC2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00002023001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To endonucleases of group I introns of fungi and phage.</text>
    </comment>
    <dbReference type="EMBL" id="FN430262">
        <property type="protein sequence ID" value="CAZ83565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839374.1">
        <property type="nucleotide sequence ID" value="XM_002839328.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGC2"/>
    <dbReference type="STRING" id="656061.D5GGC2"/>
    <dbReference type="GeneID" id="9183166"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002023001"/>
    <dbReference type="eggNOG" id="ENOG502QZTK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055331_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGC2"/>
    <dbReference type="OMA" id="LFRGEWY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10445">
        <property type="entry name" value="GIY-YIG_bI1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1440.10">
        <property type="entry name" value="GIY-YIG endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000305">
        <property type="entry name" value="GIY-YIG_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035901">
        <property type="entry name" value="GIY-YIG_endonuc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006350">
        <property type="entry name" value="Intron_endoG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003647">
        <property type="entry name" value="Intron_nuc_1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010896">
        <property type="entry name" value="NUMOD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003611">
        <property type="entry name" value="NUMOD3"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01453">
        <property type="entry name" value="grpIintron_endo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01541">
        <property type="entry name" value="GIY-YIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07453">
        <property type="entry name" value="NUMOD1"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="NUMOD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00465">
        <property type="entry name" value="GIYc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00497">
        <property type="entry name" value="IENR1"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="IENR2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64496">
        <property type="entry name" value="DNA-binding domain of intron-encoded endonucleases"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF82771">
        <property type="entry name" value="GIY-YIG endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GIY_YIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GIY-YIG" evidence="5">
        <location>
            <begin position="36"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ83565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="299" mass="34748" checksum="2CC1EE1412039BEC" modified="2010-06-15" version="1">MSTSWFLTKAIEQIKPVKVYNNFKGDRLQLLKDQKDKVGVYYLVNLINGHSYIGSSMNLAGRMRNYLNNTFLKHKKNCNIPIVEALLKYGQDNFAVLIIEYVDVKELTIRETYFLRKLLPYYNVLKQGYSSIGYKHTEATKQMLSELAKNRIHSDQTKALISRALTGENNPFYNKTHSVETKLRMIEANSAYPVYIYNSYRELLVVFPSVTTLAKLINSNHSTIVSYIKNKVLFREIKNSSHIKKAIFVYNTNKEFICKLDGVTHAEREFSISHEIIKKYALLNKAYGGYIFSYERLKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGG1</accession>
    <name>D5GGG1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007371001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430264">
        <property type="protein sequence ID" value="CAZ83604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839413.1">
        <property type="nucleotide sequence ID" value="XM_002839367.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGG1"/>
    <dbReference type="GeneID" id="9183202"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007371001"/>
    <dbReference type="HOGENOM" id="CLU_1548738_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGG1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39609">
        <property type="entry name" value="RFEG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39609:SF2">
        <property type="entry name" value="TRANSCRIPTION FACTOR RFEG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="128"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="173" mass="19440" checksum="51056E63923976EA" modified="2010-06-15" version="1">MDYTTPSPIPYREENLMTYRMSAEGIEWEVLQDCINSFLGPEASASLERTQDGEDVYWVTATRPFLPSQINDLKAFSAEFLEDRDAAASKHRRPSHHPRNRGMRRQKQATSPIHVQLVLPSLHQNAYLMIPPPSSSPASSNHCRNCQNRPPHSPHHDHHPHSSAASSQAGTLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGH6</accession>
    <name>D5GGH6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00007387001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430264">
        <property type="protein sequence ID" value="CAZ83619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839428.1">
        <property type="nucleotide sequence ID" value="XM_002839382.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGH6"/>
    <dbReference type="FunCoup" id="D5GGH6">
        <property type="interactions" value="1143"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GGH6"/>
    <dbReference type="GeneID" id="9183217"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007387001"/>
    <dbReference type="eggNOG" id="KOG3836">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002768_1_0_1"/>
    <dbReference type="InParanoid" id="D5GGH6"/>
    <dbReference type="OMA" id="HPMMRRI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00102">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057962">
        <property type="entry name" value="SPT23_MGA2_DBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23335">
        <property type="entry name" value="CALMODULIN-BINDING TRANSCRIPTION ACTIVATOR CAMTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23335:SF1">
        <property type="entry name" value="CALMODULIN-BINDING TRANSCRIPTION ACTIVATOR, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13857">
        <property type="entry name" value="Ank_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25603">
        <property type="entry name" value="SPT23_MGA2_DBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01833">
        <property type="entry name" value="TIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00429">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1010"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="domain" description="IPT/TIG" evidence="5">
        <location>
            <begin position="432"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="2">
        <location>
            <begin position="623"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="2">
        <location>
            <begin position="656"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="166"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="340"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="557"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="699"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="918"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="16"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="171"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="208"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="229"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="251"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="357"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="375"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="718"/>
            <end position="742"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ83619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1081" mass="117453" checksum="D3260E2640B572F0" modified="2010-06-15" version="1">MRKPELLQRALARARSSGEDSDNKDSIPRSDESPSDEESPLMGGEVKICVGCISRERKRAARKKPKQIKEEEEWMMDEPKRAVVFNNHEVREWLPTSGIQSLNGGMDGSGVLGSWEDGMIMELHMRLACYCRHQGEKEGFRVIFTIKNHEDVVIAQQITSSILITDDHKSSASTPSINGGSQDIKLVGTGYQPPQNQLPPNIFGTPVGSISSHDNQQSFGAMGSGAHGSTPQSPLFSQSSFSPPPPFGRNMSPTSPMASTGSHQQSPPPQLPSGNPSTKKRRMNSVPKVPVGLTMTRMDNPCPSFQQQNGPLIANNQPTQLQAPALNPFSVAQSPQAFWADEPMTPSGMSDHAGFQFDSTPSTNFNGLDNRSQFASPQSARPSRAPSPSPQEAFLRAQQAASRVPMHPQSQVRHVQTHNFQAVPPESEASQLPEVSRLVPQEGTCRGGIEVTVLGRSFVPGLTVLFGGTPASYNFFFSPTTMVCLLPPNASPGPVVVSIRNVQLGTGADKVRFFTYINDADKSLMELALQIVGMKMRGTMSNPQDIARSIIEGDTGSAFSQGSPRASQDGTSGSQQHHQYLRRLAGESGFGRHGLEGTLLKFLDAMDLDESPYPFRLDLTNKAGQTMLHLASMLNMGRFAAALLARGASVQAADANGYTPLHFAALHRHLEIYRRLLRHNANPNLQTRNGDTAIDLAFKQGSSRGRSQRDTRSRHQSRASSTSSEGHFGSRSRAGSSASLSAMWDPSGMETGRHKYVGVDDFFGAADESASGDGDDSVDDRWMHSRRGSRHETTAVNASLGDNVLATDRAPPVPGSMPPPTEGLAPAAAVTAWREQIAASIQNLQNLHWNLPAMPTAADYQHIFQNPMAALQNPMARFHALMPPSPFAQDRDVVNSPEIKKGQYTWWELFSAPQAPPAYDELFPHQPGGSHGPPGDIVLERGSAADNAKLLEEEELVPPGSSGSLSSASSLDVNELRYRIARGVQDMTKEQQDEYRAHARKMKKIESDRRLYFFWLPALILILMAMAMSYGPAVWYALKVIVSFVEAVFEDPSSIKESMAAMASGVAVAGGFLEPRVVPVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGI9</accession>
    <name>D5GGI9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">GSTUM_00002039001</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the ZNF598/HEL2 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="FN430269">
        <property type="protein sequence ID" value="CAZ83632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839441.1">
        <property type="nucleotide sequence ID" value="XM_002839395.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGI9"/>
    <dbReference type="FunCoup" id="D5GGI9">
        <property type="interactions" value="71"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GGI9"/>
    <dbReference type="GeneID" id="9185556"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002039001"/>
    <dbReference type="eggNOG" id="KOG2231">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008515_1_0_1"/>
    <dbReference type="InParanoid" id="D5GGI9"/>
    <dbReference type="OMA" id="VFTHEHT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16615">
        <property type="entry name" value="RING-HC_ZNF598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057634">
        <property type="entry name" value="PAH_ZNF598/HEL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041888">
        <property type="entry name" value="RING-HC_ZNF598/HEL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056437">
        <property type="entry name" value="Znf-C2H2_ZNF598/HEL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044288">
        <property type="entry name" value="ZNF598/HEL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22938:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE ZNF598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22938">
        <property type="entry name" value="ZINC FINGER PROTEIN 598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23202">
        <property type="entry name" value="PAH_ZNF598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25447">
        <property type="entry name" value="RING_ZNF598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23230">
        <property type="entry name" value="zf-C2H2_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="14">
        <location>
            <begin position="111"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="380"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="439"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="509"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="626"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="26"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="380"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="453"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="484"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="626"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="647"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="681"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="709"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="732"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="793"/>
            <end position="802"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00035113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAZ83632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="802" mass="87774" checksum="31660C5C1B09124E" modified="2010-06-15" version="1">MSSSTDTLVPIQNQTLADTPQGGSRGRGRGTRGGRSRSRNENRVDSTLDGGSRGGRRGWRNGRIGRQPPRASDQPVDRQIQADLIRASMTARGEIRPDADDAASDPDSSVCFICADPVQFSSIAPCNHVTCHICALRMRALYKNKACAHCRTESEWVIFSNTDSKRFQEFKDEEIIDRNDALGVKFENQTILDETTLLLRYNCPDPSCDRACRGWPDLYYHVRVAHSRVLCDLCSRSKKVFTHEHTLFTAQELRKHERVGDDQPGSENQSGFKGHPECAFCRQRFYSSDELYVHCREKHERCFLCDRTNPSTQQQYYADYDSLVVHFSNDHFMCGEQGCLDKKFVVFDSEMDLKAHQLEVHPSLLSKGALRDARRIDMRTLEDRSHHSQNRRDGGGGHGRGSGRVEMEEPIRTEQQMSRAELAYYRTLAVQGAQSTTPRIFGGQLTEPPPVIRPSAQATARAPAQPPSSITRPSAQADGMSVSTPPPRPPPRPADTSTAEAAFPPLAAPRAQAEPSPGMGGPITGDNRRVHHGAVIERANTMLRYNQSKFDSFKGHVSSYRQDKISAGELVELFWSLFDVKAPDLGKLIKELADLYDDEGKKQGLLKAWNNWKAINEDYPPFAGASSGISSSDPTQPSSSCVLKLKSSTSHSARSTTARQGAWGSAAAAPPPPLPSQLQSTSRTPPLSVNRSGPGAHAPTPWVAPSGPPTASSSTIQSNISTTRREDFPSLPTKPPPPPGWGPIKRREHGAPAPTVDPWGRSAGTGSGVGFAEVSDVEGNGSGGGGRKKKGKQKEVLFRVGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGM8</accession>
    <name>D5GGM8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007429001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430275">
        <property type="protein sequence ID" value="CAZ83650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839459.1">
        <property type="nucleotide sequence ID" value="XM_002839413.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGM8"/>
    <dbReference type="STRING" id="656061.D5GGM8"/>
    <dbReference type="GeneID" id="9185572"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007429001"/>
    <dbReference type="eggNOG" id="ENOG502S19W">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005247_1_1_1"/>
    <dbReference type="InParanoid" id="D5GGM8"/>
    <dbReference type="OMA" id="DSARIWA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="304"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="112"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="306"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="326"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1005" mass="113813" checksum="9ABE8A8926164D85" modified="2010-06-15" version="1">MSLPILGLPLHRVLLRRPLSYTLLSRLDWIVQGGCLDTGKLQVKGLGRNSASELTEGCGKGKAGLEREIDDGDFKAGGGVKGRNAAQNKNSRENPERKLGGRAAGKKRWGRDGVSSSNGGDDSLLSLLRSTGHARDIPRKASGKVDLSHVDPLEPRRRSNKDRYRLAEPGRALKRSYFGGVKVRYIDQDGIQRERYSLKSITMSLIYHIYKLYPHSSFRFPVLESAGNLSEDHDEMVLRGVFTSGARDYLEQRGYTPEDVLVWAWVLTARDGGLAGHRLDLGTRVRNEMVDRSLEEQDILDAELPGEERVQSSHGGEDLDSDDTKNMQLSSPRNTTGARHTYRIPPFLILFTLRRKYFIRDNVRALFPMIESVLFPSNHLSISEDKTTVLLLLRLLRRVRSTWPPAISIVAKLLVTHLHRPDTSRPRSPSRLTRLYNRLLTCFSLPTPDGPFAHLPILQRSQFLILRKMAAMGIPLVREGYRAIVIVQLAHAKTPPEKQTVRDMVTTWPPWPFERDGREGLPPRISRAAEVIQRMTEAGYPLHDWEYSALVLAGKDTDNTPTIQTRTFHLRLRHLISSSPTSIWASRIRATRTIDEAWAIFLDCRQNGTIPRRDVWEELIAKALYAEKLTRQQMHADCIKRKRPLCEKPRLDTLSRLQRESNYRVVAGEAKEVLPPPRNINEGVYVPIPPPDTDELFMMMKRDNVPPSPRLLALLISHSSDLNYAHRILETWGYPLPSPSPSAKPTDPRILTAYISLLLHRRNDEIELAIRLLLAHKLTYTPAYNATLTRLARTLDSRKANDKPNRIRIIWEVYKKMTRLVSEDSQTLRALCVAAEKNIRLGANVLWDGIDPVDQVIHIFETNIGLHKPKLLIADEELPPLLTPSPATLHAYVRLLGFSRRYRDIGRIIEWAAAADWLDDGDKPMARRVMVAARVFLEVPAGKQGEQHQQGEESEDSVRREVLRDIGILVREWPDGEEVAEYCLLGGLRWISPQVGDGKGGCTVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGT5</accession>
    <name>D5GGT5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00007505001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430275">
        <property type="protein sequence ID" value="CAZ83707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839516.1">
        <property type="nucleotide sequence ID" value="XM_002839470.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGT5"/>
    <dbReference type="FunCoup" id="D5GGT5">
        <property type="interactions" value="866"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GGT5"/>
    <dbReference type="GeneID" id="9181677"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007505001"/>
    <dbReference type="eggNOG" id="KOG0789">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001645_9_12_1"/>
    <dbReference type="InParanoid" id="D5GGT5"/>
    <dbReference type="OMA" id="WSIDKAP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18533">
        <property type="entry name" value="PTP_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050348">
        <property type="entry name" value="Protein-Tyr_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134:SF449">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="3">
        <location>
            <begin position="52"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="4">
        <location>
            <begin position="249"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="343"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="27"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ83707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="373" mass="43008" checksum="A894A59DF8EA18BC" modified="2010-06-15" version="1">MNTHLPRAGPQSLHRSIPPSISLQRPSRSRFRHGRSREKGTPGFLTQTVADIRRKFLELELLQSERLRDNEDTQWKIDDSNEVRIRNRYCNIAPWDSNRVRLRVPTEHCDYINASPIVLKGRNGSIKRYIATQGPKEGQFNHIWRMVWQETSDVAVIVMLTKTFELGRDKCFQYFPEKLDDKPWTVDGDEEFGDGFKATISLLEKTKDKRSSCTVRKISLKVGEKEKIVWHLLFKGWPDFNVPEGDNRSALLEAIKLANEKNSGPQNPLIVHCSAGVGRSGTFITLDHFLREIDAGTISADEREDPVFEVVNRLREQRMYMVQSQVQYEFLYQVLKERFQRRAKSASPPTVPLDDPAETDSEFENGQGGVRLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>D5GH99</accession>
    <name>D5GH99_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00007713001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430308">
        <property type="protein sequence ID" value="CAZ83851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839660.1">
        <property type="nucleotide sequence ID" value="XM_002839614.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GH99"/>
    <dbReference type="STRING" id="656061.D5GH99"/>
    <dbReference type="GeneID" id="9182556"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007713001"/>
    <dbReference type="eggNOG" id="ENOG502QYDH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007183_1_0_1"/>
    <dbReference type="InParanoid" id="D5GH99"/>
    <dbReference type="OMA" id="NTWHEAP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.10">
        <property type="entry name" value="Bactericidal permeability-increasing protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027842">
        <property type="entry name" value="HAM1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045967">
        <property type="entry name" value="HAM1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31138">
        <property type="entry name" value="CHROMOSOME 19, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31138:SF1">
        <property type="entry name" value="PDZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14613">
        <property type="entry name" value="HAM1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19343">
        <property type="entry name" value="HAM1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HAM1-like N-terminal" evidence="3">
        <location>
            <begin position="3"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="domain" description="HAM1-like C-terminal" evidence="2">
        <location>
            <begin position="642"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="167"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="206"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="791"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="206"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="233"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="793"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="810"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="885"/>
            <end position="894"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ83851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="894" mass="101252" checksum="F6A5DE13BF962706" modified="2010-06-15" version="1">MDSRVNRPVDPKQRERDVENKLRLYGIYTAFQNGRLPSNNQIDVALSSLTNHSKLRSPNENLSAEGKIILEDFRTTVEEAKRLVLVKNHDQVFQEFIWNTTQLGVKGGPQTSTPGVPVSKETANRDVEQAQAGFRTLGQLITTNGEFRKLLNDAVVLLRDIAGDAATKTASRISPTEDQLRQMDNPAPDHQWVDAPNLSRENFKSQVRSQFNQNKPVPRGEAVGSATQATDPHGSRDTRDATARTAHDERKNRDAGGLDASSGIRAGAEELRQKAQDNVPEGQKQRVPEHMERASNYMQNKMPQERREQVIFRLKKMVVEIQSHQDYQQAIDTLLGLAETYTGHTKGIASNSTGTVKDAHQDTHLQSSEKSLRVILERFANYTSIEDLIDSINDIYRDANNDPELKGWFGDVNNYIRTCLKEEGYIMRHESTEQYDRLYDHGNFLLRNRYRDHTDRVANELRFLADQFAADPENQRFRQATQKLFTDLGNDENGKPVFKKHLVKDITQIIIPELIENIRYIPFPRIEYSDPMIDAVVENLIIESDNLMPNVLEIGNDNYFRFGRKTVSSKHSHAAMVSASQIQCDIRDVSYYIKKKQGFPSITDTGVADIFLGGDGFNFRLQLATPSKLDRARFFKVERVDVNINTLKIKLKKSNHKIFFALFKPVLMGIVKPAITKILEKQIRDSFGRLDQLAYAIYQEEQKIERDIKKNPDPTHVQNIYSRYYQAARRELANRKQKAEAKVADKHANVAVTQDDSMFKNISLPGGISTKATEYRDLSKRGDRWCSEVFTIGSASPTPNLPEPQQITRKSPHAHRRTTRGRETTSDGGASRDSGYHANDYHGYGTGTGNTGFSDKLADGQPDSVGNRKTGEYSLNRPLDENYATGHSRTTPVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHK3</accession>
    <name>D5GHK3_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 16">ADP-ribosylation factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">GSTUM_00007957001</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">GTP-binding protein involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.</text>
    </comment>
    <comment type="function">
        <text evidence="16">GTP-binding protein involved in protein trafficking; modulates vesicle budding and uncoating within the Golgi apparatus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430319">
        <property type="protein sequence ID" value="CAZ83996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839805.1">
        <property type="nucleotide sequence ID" value="XM_002839759.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHK3"/>
    <dbReference type="FunCoup" id="D5GHK3">
        <property type="interactions" value="1119"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GHK3"/>
    <dbReference type="GeneID" id="9183650"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007957001"/>
    <dbReference type="eggNOG" id="KOG0070">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040729_9_3_1"/>
    <dbReference type="InParanoid" id="D5GHK3"/>
    <dbReference type="OMA" id="HYYANTN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04150">
        <property type="entry name" value="Arf1_5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003500">
        <property type="entry name" value="ADP-ribosylation factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045872">
        <property type="entry name" value="Arf1-5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 16" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8 16" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 13 15" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10 16" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 16" id="KW-0519">Myristate</keyword>
    <keyword evidence="5 13 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 16" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 16" id="KW-0813">Transport</keyword>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="24"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="126"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00053326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00070396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU369003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAZ83996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="181" mass="20638" checksum="1B63AE20970DCDB2" modified="2010-06-15" version="1">MGMSMSKLFTGLFGKREMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGIIFVVDSNDRERVSEAREELQRMLNEDELRDALLLVFANKQDLPNAMNAAEITDKLGLHSLRNRAWYIQSTCATSGDGLYEGLEWLSSNLRARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHM9</accession>
    <name>D5GHM9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008051001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430320">
        <property type="protein sequence ID" value="CAZ84059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839868.1">
        <property type="nucleotide sequence ID" value="XM_002839822.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHM9"/>
    <dbReference type="GeneID" id="9183713"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008051001"/>
    <dbReference type="HOGENOM" id="CLU_2374304_0_0_1"/>
    <dbReference type="InParanoid" id="D5GHM9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="95" mass="11194" checksum="91BE49542CABFAC4" modified="2010-06-15" version="1">MRKGTGILRGIEAGGIEIEAVVGTEKSWTRKQRLKRSDYVIFRKETNLQSASKRRMRRRPRSWLKTGHPRRKVNSSLNGELWQMTLQLELRPFQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHR3</accession>
    <name>D5GHR3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00008004001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the actin family. ARP2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430320">
        <property type="protein sequence ID" value="CAZ84024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839833.1">
        <property type="nucleotide sequence ID" value="XM_002839787.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHR3"/>
    <dbReference type="FunCoup" id="D5GHR3">
        <property type="interactions" value="1007"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GHR3"/>
    <dbReference type="GeneID" id="9183678"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008004001"/>
    <dbReference type="eggNOG" id="KOG0677">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027965_0_0_1"/>
    <dbReference type="InParanoid" id="D5GHR3"/>
    <dbReference type="OMA" id="WEDMQHL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051285">
        <property type="term" value="C:cell cortex of cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031097">
        <property type="term" value="C:medial cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044396">
        <property type="term" value="P:actin cortical patch organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030476">
        <property type="term" value="P:ascospore wall assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051654">
        <property type="term" value="P:establishment of mitochondrion localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000001">
        <property type="term" value="P:mitochondrion inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd10220">
        <property type="entry name" value="ASKHA_NBD_Arp2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000050">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000005">
        <property type="entry name" value="Actin-related protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020902">
        <property type="entry name" value="Actin/actin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00190">
        <property type="entry name" value="ACTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01132">
        <property type="entry name" value="ACTINS_ACT_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ84024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="388" mass="43925" checksum="F2F18A17323C4189" modified="2010-06-15" version="1">MNEAPIVLDGGTGFLKVGYAGQNFPEHQYPSIVGRPILRAEERAGDMIVKDIMVGDDAAAVRTMLQITYPMDNGIIKRWEDMQHVWDYTFSEKLQVDTTGRKILLTEPPMNPLSNREKMCEVMFERYNFGGVYVAIQAVLALYAQGLSSGVVVDSGDGVTHIVPVYESVVLNHLTRRLNVAGRDVTRQLINLLLRRGYAFNRTADFETVRQIKEKLCYVSYDLELDHKLAEETTVLVESYTLPDGRVIRVGSERFEAPECLFQPHLVDIEQPGIAEFLFNTIQAADVDVRTSLYKAIVLSGGSSMYPGLPSRLEKELKQLWLTKVLHGDPTRLSKFKVRIEDPPRRRHMVFLGGAVLANIMADKENMWITKAEWEEQGPRIIEKLGPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHU9</accession>
    <name>D5GHU9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008128001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430321">
        <property type="protein sequence ID" value="CAZ84092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839901.1">
        <property type="nucleotide sequence ID" value="XM_002839855.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHU9"/>
    <dbReference type="GeneID" id="9184185"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008128001"/>
    <dbReference type="eggNOG" id="ENOG502S5QM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044725_0_0_1"/>
    <dbReference type="InParanoid" id="D5GHU9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39599:SF2">
        <property type="entry name" value="ANCHORED PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G09650)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39599">
        <property type="entry name" value="GPI-ANCHORED PROTEIN (EUROFUNG)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072744" evidence="1">
        <location>
            <begin position="24"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="398" mass="41480" checksum="4C039040BA087410" modified="2010-06-15" version="1">MKTTTSAFFIQFLLTATPFLAAASAPYRWPAGIDPSKKYWPPGEGPPSKRWLNELAEAFNLNQSFTPESISRAFAVKKIMDPAEKFMPEYWNWNGNGAEGEGKGQQVMVANLTFNSPVRRNVFDSYAYGGLFKRQSFQCAIGTHACSNIGRSQSCCNNNEQCFKIEDTGFGDVGCCVNGQTCSGNLKGCGDDKKTCSSREGGGCCTEGYNCSAQGCVPAEGQVVSSSTTPTPTSSTSPSITCSSGYYACPSNLAGGCCPSGRLCGVSDCPEQTITATPVASGDTCPTGYYTCSAQFNGGCCRIGRDCGITDCPERAITISSGTSQGAPAVTTGGRCASGWSSCPNNLGGGCCPAGYTCARENCPAVTLTGSNIVVSGAVGSAFFLSIYAKNTNGRGRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5GI85</accession>
    <name>D5GI85_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008331001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430324">
        <property type="protein sequence ID" value="CAZ84228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840037.1">
        <property type="nucleotide sequence ID" value="XM_002839991.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GI85"/>
    <dbReference type="GeneID" id="9181946"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008331001"/>
    <dbReference type="HOGENOM" id="CLU_3392570_0_0_1"/>
    <dbReference type="InParanoid" id="D5GI85"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="32" mass="3682" checksum="8AC9F9B6FE6EA0B2" modified="2010-06-15" version="1">MGFVFKFLLTAFFIGEAIYLISNWAAARAERK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIB6</accession>
    <name>D5GIB6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008401001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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            <end position="83"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="237" mass="25287" checksum="AB9042483F5005F3" modified="2010-06-15" version="1">MDTARTTATATATTTPLFTTPATSTASRLKRRSTVHTAIAANNVHVSPYPTSPRSSEPPCKPTSSLARKRRSSIMSRGSMSPGGSSGSGWIEVGGCENRGGGGDVDMAREGVEEGFSKISQAMNLLSLQPTPTPTPTTPSNFDAPVHWPSPEELLKESLRQSLSALHALSQIKYLVEKSPSSIDEQNGEAGGGGVSEVMGVYDRAMHEMRAAAEILAWAERHLSEEKEIVRERLLKG</sequence>
</entry>
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    <name>D5GID9_TUBMM</name>
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            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
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        <lineage>
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        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
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                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
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                <person name="Da Silva C."/>
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                <person name="Ghignone S."/>
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                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
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                <person name="Ruotolo R."/>
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                <person name="Lebrun M.H."/>
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                <person name="Wincker P."/>
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            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">Mel28</strain>
        </source>
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    </dbReference>
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            <end position="28"/>
        </location>
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            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84282.1"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="133" mass="14565" checksum="D2C8BB3687553707" modified="2010-06-15" version="1">MAPSGFQTQSEITNGRSLERSHRPGYGLENNQQLVAVIPQPPGEARVVAQDQGGWWIPAGLWKWILHFINNAPAPHPGRQDGQTETRPTHAEHVGDRRENSGTIEGSYNTTNYSGNAKSFGQNGKVTINGDIH</sequence>
</entry>
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    <accession>D5GIP4</accession>
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        <lineage>
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            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
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            <taxon>Tuber</taxon>
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    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
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                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430328">
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="interactions" value="336"/>
    </dbReference>
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    <dbReference type="KEGG" id="tml:GSTUM_00008596001"/>
    <dbReference type="eggNOG" id="ENOG502QQGC">
        <property type="taxonomic scope" value="Eukaryota"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0005634">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.240.10:FF:000002">
        <property type="entry name" value="Zn cluster transcription factor Rds2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PAS_13"/>
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        <property type="entry name" value="Zinc_cluster_trans_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31986">
        <property type="entry name" value="REGULATOR OF DRUG SENSITIVITY 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31986:SF7">
        <property type="entry name" value="REGULATOR OF DRUG SENSITIVITY 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24990">
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="9">
        <location>
            <begin position="83"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="278"/>
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            <begin position="28"/>
            <end position="40"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="8">
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
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    <feature type="compositionally biased region" description="Pro residues" evidence="8">
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    <feature type="compositionally biased region" description="Polar residues" evidence="8">
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        <source>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ84387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="548" mass="60031" checksum="A69E05A801847F66" modified="2010-06-15" version="1">MTEVHPPASASSNGAAVRAPHPSAQQSNGGGVGTSQGGASGNSASSLGTVGAEPSGSQSPTTSTPGQTSMGRSPKKRRKVNHACVYCRRSHMTCDDDRPCMRCVKRNIGHLCHDEPREPKKSKTESSTTTQENDQEDSNTSPSKASVSPPVNGMSKPLEQQEVRASTATTASNVSLPPPPIPPSRPGAGTSIVAPSPVSVAPRAALNGGSQAFLGVTDWDHPLPQQQATSYTNPYHHQWMFSAPEVSSEFSLLSDFLGNLYPDEIFTADQNQSLFSDTSSNVQLGQSGTSNTTFNQQRLLPPVNPNSTSSIPRPGSAKPSTERAKENYYLTAADPSQDAPEERMKQVLKAKLEAGLLKPFNYVKGYARLSQYMEKNMAHSSRRRILAQLDKFRPKFRERTHQLTDLDLVHVEEWFEKSLLEYDRVFASMAVPACLWRRTGEIFRGNKEFAELISVPIEQMRDGKLAIYELVVEDSAVSYWEKFGTIAFDTTQKAMLTSCHLKNPDPNAKEKLIHCCFSFTIRRDMNDIPALIIGNFLPIHPKKHGFGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIR2</accession>
    <name>D5GIR2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008620001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430328">
        <property type="protein sequence ID" value="CAZ84405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840214.1">
        <property type="nucleotide sequence ID" value="XM_002840168.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIR2"/>
    <dbReference type="STRING" id="656061.D5GIR2"/>
    <dbReference type="GeneID" id="9185623"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008620001"/>
    <dbReference type="eggNOG" id="ENOG502QTKU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028866_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIR2"/>
    <dbReference type="OMA" id="EITQVAH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006740">
        <property type="term" value="P:NADPH regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42840:SF7">
        <property type="entry name" value="BINDING ROSSMANN FOLD OXIDOREDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G10190)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42840">
        <property type="entry name" value="NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase N-terminal" evidence="1">
        <location>
            <begin position="5"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="366" mass="41129" checksum="DA40B122C560F287" modified="2010-06-15" version="1">MSTPINIGIIGCGEVAQVVHLPTLAFLPELYRITYLCDISPSAVSHAASKISSQQPIHTTQKAAELCSSPLVDAVFILGPEEFHAEHALLALIHDKHVFVEKPMTLTKKDALAIAEGEKHSKGRVMVGYMRRYAAVVEDAIAEIGGRENIIYARVRDIIGPNSTFVEQSGTFPRKFSDHAPEDAGEFARRTKDMISGELTGYGVPVTQESIAQYRRVTKLGCHDLSLMREVLGMPVCENPTLGASLRLTVWNVLFNYGHFTALYESGFHQIPIFDAHIEVYSENKSVLIQYDTPYVKGLPVTMTIRENVDGKFVERFVRNTYEDPYTLELKNFYHAIREGGKIKTDVGDSLEEFELFEMIMKNWRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJ90</accession>
    <name>D5GJ90_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008886001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840392.1">
        <property type="nucleotide sequence ID" value="XM_002840346.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJ90"/>
    <dbReference type="STRING" id="656061.D5GJ90"/>
    <dbReference type="GeneID" id="9185951"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008886001"/>
    <dbReference type="eggNOG" id="ENOG502RJ2Z">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_845169_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJ90"/>
    <dbReference type="OMA" id="NWGNDEL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="178"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="287"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="329" mass="35176" checksum="B7842AEAC7F02BED" modified="2010-06-15" version="1">MRTDPRIRQSLNRISTSLENASDNARTGCFTFAKSYLDPCLASLSNCLPRQRPHNAHHRRSEYPFGFYDYDDYSDEETSRRGGGFLAWGNDELDRLLAGSGAGDCGGGGSGSSSGYRAGGGMYYGSSASTSAVGALGGKRKCNLSNESDPTVIPTTSMFGFLARFTSFRGRGLRYKPSAANLQEHPQRHTGLTAVEEESGRNRSTSPGIRGGRGGAGRGRKRSATNSSGESAGESLRSRGDLWPSEDDDDAIPISDDFLGNSTGGDLVVVRSRDEASPSPPLPSDDDALRLEEQTAREYEESEIERKRGAAKKLALQRGLAASPVSPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJ94</accession>
    <name>D5GJ94_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">General transcription and DNA repair factor IIH subunit TFB4</fullName>
            <shortName evidence="14">TFIIH subunit TFB4</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13 14">RNA polymerase II transcription factor B subunit 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">GSTUM_00008890001</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 14">Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to TFIIK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module TFIIK controls the initiation of transcription.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Component of the 7-subunit TFIIH core complex composed of XPB/SSL2, XPD/RAD3, SSL1, TFB1, TFB2, TFB4 and TFB5, which is active in NER. The core complex associates with the 3-subunit CTD-kinase module TFIIK composed of CCL1, KIN28 and TFB3 to form the 10-subunit holoenzyme (holo-TFIIH) active in transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the TFB4 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840396.1">
        <property type="nucleotide sequence ID" value="XM_002840350.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJ94"/>
    <dbReference type="FunCoup" id="D5GJ94">
        <property type="interactions" value="1103"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJ94"/>
    <dbReference type="GeneID" id="9185955"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008890001"/>
    <dbReference type="eggNOG" id="KOG2487">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040211_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJ94"/>
    <dbReference type="OMA" id="QGCDITS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004600">
        <property type="entry name" value="TFIIH_Tfb4/GTF2H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12831:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIH SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12831">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIH TFIIH , POLYPEPTIDE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03850">
        <property type="entry name" value="Tfb4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 14" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11 14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 14" id="KW-0804">Transcription</keyword>
    <keyword evidence="9 14" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8 14" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="331"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="337"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00033341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU368090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAZ84587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="357" mass="38387" checksum="0BD3EC565709189A" modified="2010-06-15" version="1">MDTVDASAHYSQSVDEKPSLLVLILDTNPYAWSTLSATLPLERALASILIFLNVHMAFSHANRVAVIASHTRSARFLYPTTTSTTTAAGGSGGSEDPFRDANKYRQFRLIEDQVQASFAELMRQTTASELVDAKETMMAGALGLALGYVNRLVRVDDSGGVVGGKDGAVGEVTSMNARILVVSVSGDLAEQYVPVMNSIFAAQRKKIPIDICKVAGETVFLQQASDTTRGIYMQLEHPESLLQYLMMCFIPDTATRRHLVPPTQVNVDFRAACFCHKNVVDIGFVCSICLSIFCTPPEGAICTTCNTKLDISALGNFGGKPAVVLTKKKKTKKLKQSKATSFSNEASATSTPGTPAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJB1</accession>
    <name>D5GJB1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00008919001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430330">
        <property type="protein sequence ID" value="CAZ84604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840413.1">
        <property type="nucleotide sequence ID" value="XM_002840367.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJB1"/>
    <dbReference type="STRING" id="656061.D5GJB1"/>
    <dbReference type="GeneID" id="9185972"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008919001"/>
    <dbReference type="HOGENOM" id="CLU_2943507_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJB1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ84604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="60" mass="6460" checksum="D3AE86BF99FE573A" modified="2010-06-15" version="1">MPSSPTRPFRTSSIRTFKPTVSSLLLSSKALADLEEKGVIKKVVGHSKLSIYTRAVAAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>D5GK42</accession>
    <name>D5GK42_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00009356001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430335">
        <property type="protein sequence ID" value="CAZ84885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840694.1">
        <property type="nucleotide sequence ID" value="XM_002840648.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GK42"/>
    <dbReference type="STRING" id="656061.D5GK42"/>
    <dbReference type="GeneID" id="9186922"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009356001"/>
    <dbReference type="eggNOG" id="KOG2440">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1428961_0_0_1"/>
    <dbReference type="InParanoid" id="D5GK42"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ84885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="190" mass="21164" checksum="FDA3628D1CFA22C3" modified="2010-06-15" version="1">MTGPTPDQKNLHYFVSGVSSISLIAPTEELFYTTVAFYTDLGFDEAFVYDRTHPTVKREDDHCRASEKETWLYSQGAAGDGTDVNLKIRYVVEAGLRRQSLIPPPIPPNSEEWRGKSMSVILYTPALDRVIDLLSEKKIAFQSQPSEKEPSELDLLLKQIRLLGSGPGMFQASWGNQGFGTRRDPAPQAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>D5GK45</accession>
    <name>D5GK45_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00009359001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat striatin family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430335">
        <property type="protein sequence ID" value="CAZ84888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840697.1">
        <property type="nucleotide sequence ID" value="XM_002840651.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GK45"/>
    <dbReference type="STRING" id="656061.D5GK45"/>
    <dbReference type="GeneID" id="9186925"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009359001"/>
    <dbReference type="eggNOG" id="KOG0642">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009046_0_0_1"/>
    <dbReference type="InParanoid" id="D5GK45"/>
    <dbReference type="OMA" id="SKCSQEV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013258">
        <property type="entry name" value="Striatin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051488">
        <property type="entry name" value="WD_repeat_striatin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15653:SF0">
        <property type="entry name" value="CONNECTOR OF KINASE TO AP-1, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15653">
        <property type="entry name" value="STRIATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08232">
        <property type="entry name" value="Striatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 6" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Striatin N-terminal" evidence="9">
        <location>
            <begin position="41"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="551"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="729"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="171"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="314"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="60"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="17"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="106"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="192"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="209"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="244"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="354"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="368"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ84888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="808" mass="85578" checksum="A9AD1873CBBC43A1" modified="2010-06-15" version="1">MAWQSPSAHNSSPDGNNGNGGLQGNGANGGSGDGAVGTEYTLQGVMRFLQTEWHRHERDRNSWEIERAEMKARIAKLEGENRAANRLHQGFLTRIKMLETALKKERTSRGLEGAERDGQGGQKAAVENSINAKLSQQSIPLGESSSLYRAEAARNKSRAYLEKCLQEITYLLTPPNHPPPQHQPPINVESMLQQQQQQQPQHQVLQQPSPSPNHQPPCPTGQASSQLGNAGMLSGHQPYPHSRASLSRSHEMGSQQASFHQELQIQPQPQINTPSGSNEQLDETLEQQNAFSSSGLDYGKADTNPFSASVATAGESATGSLAPGDTDSWDFDETTRVGGSDLEAANSHPKEIEQYSSSNHTGKANSVNRRKLSVSKHRSPSESRDSAGTVASTTVLGSDSGNFKLKFALRGHLDVIRAVVFTGGGTANEPEICTAGDDGVLKRWFISRSYGLSMGLSDMDIQSHFTHRGHSGMVTCLAARPPAGGSGGEAASNAGGWIFSGGQDSTVNVWEAGKVGPKATLVGHTDVVWAVCVLPSASNVLGSSDSFERVLLASGSADGTVKIWSVTPPPSPQSHSNAGSMLGSFRGTPSSTVPYTDNVPFKYSLISTITREGIIANPTCITPMSITGETFVVSYDDAAIIIYDTASGEEIVSMVSQETYDGTPASGVNAVVASTVSLESAVEAGKGDEALMAGATGLKGGVGGMIISGHEDRYVRIFDANSGQCTYSMLAHPAAISSLALSPDGRELVSAGHDASLRFWSMEKRSCTQEITSHRLMRGEGVCSVVWSIDGRWAVSGGGDGVAKVFAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKI4</accession>
    <name>D5GKI4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00009575001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430339">
        <property type="protein sequence ID" value="CAZ85027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840836.1">
        <property type="nucleotide sequence ID" value="XM_002840790.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKI4"/>
    <dbReference type="FunCoup" id="D5GKI4">
        <property type="interactions" value="122"/>
    </dbReference>
    <dbReference type="GeneID" id="9184688"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009575001"/>
    <dbReference type="eggNOG" id="KOG3292">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081702_1_1_1"/>
    <dbReference type="InParanoid" id="D5GKI4"/>
    <dbReference type="OMA" id="IQFIGHY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046521">
        <property type="term" value="P:sphingoid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009305">
        <property type="entry name" value="Mpo1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28026:SF9">
        <property type="entry name" value="2-HYDROXY-PALMITIC ACID DIOXYGENASE MPO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28026">
        <property type="entry name" value="DUF962 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_8G05310)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06127">
        <property type="entry name" value="Mpo1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="193" mass="21541" checksum="3941DF7E3FA83EDE" modified="2010-06-15" version="1">MGILDLEQQLIFYGSYHHTKGNILIHLIGVPAIMATTFLFASNTPPLLPLPFSIPYFPPTLATLSALLYSTLYILMEPFAGLLITPLILLMTAFVTHLTSASTTTVTANTVAGYIFVLAWIAQFVGHGVYEKRAPALFDNLVQAVFLAPFFVWFEVLFWCGYRPELKVRVEEGVVRRLEAFRRKETGNVKGKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKP3</accession>
    <name>D5GKP3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00009679001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430340">
        <property type="protein sequence ID" value="CAZ85086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840895.1">
        <property type="nucleotide sequence ID" value="XM_002840849.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKP3"/>
    <dbReference type="GeneID" id="9184748"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009679001"/>
    <dbReference type="HOGENOM" id="CLU_3410813_0_0_1"/>
    <dbReference type="InParanoid" id="D5GKP3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ85086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="29" mass="3483" checksum="603CC11C7A570CE9" modified="2010-06-15" version="1">MIIYIVYFERLCSRWANTLAIHNREVSAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKS1</accession>
    <name>D5GKS1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">H/ACA ribonucleoprotein complex subunit CBF5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Centromere-binding factor 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">H/ACA ribonucleoprotein complex subunit cbf5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">H/ACA snoRNP protein CBF5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Small nucleolar RNP protein CBF5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">GSTUM_00009737001</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalytic subunit of H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Pseudouridine ('psi') residues may serve to stabilize the conformation of rRNAs and play a central role in ribosomal RNA processing. The H/ACA snoRNP complex also mediates pseudouridylation of other types of RNAs. Catalyzes pseudouridylation at position 93 in U2 snRNA. Also catalyzes pseudouridylation of mRNAs; H/ACA-type snoRNAs probably guide pseudouridylation of mRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a uridine in mRNA = a pseudouridine in mRNA</text>
            <dbReference type="Rhea" id="RHEA:56644"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:14659"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>uridine in 5S rRNA = pseudouridine in 5S rRNA</text>
            <dbReference type="Rhea" id="RHEA:47036"/>
            <dbReference type="Rhea" id="RHEA-COMP:11730"/>
            <dbReference type="Rhea" id="RHEA-COMP:11731"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>uridine in snRNA = pseudouridine in snRNA</text>
            <dbReference type="Rhea" id="RHEA:51124"/>
            <dbReference type="Rhea" id="RHEA-COMP:12891"/>
            <dbReference type="Rhea" id="RHEA-COMP:12892"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the pseudouridine synthase TruB family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430340">
        <property type="protein sequence ID" value="CAZ85114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840923.1">
        <property type="nucleotide sequence ID" value="XM_002840877.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKS1"/>
    <dbReference type="FunCoup" id="D5GKS1">
        <property type="interactions" value="1445"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GKS1"/>
    <dbReference type="GeneID" id="9183350"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009737001"/>
    <dbReference type="eggNOG" id="KOG2529">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032087_3_2_1"/>
    <dbReference type="InParanoid" id="D5GKS1"/>
    <dbReference type="OMA" id="KYGRTNE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031429">
        <property type="term" value="C:box H/ACA snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000495">
        <property type="term" value="P:box H/ACA sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990481">
        <property type="term" value="P:mRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031118">
        <property type="term" value="P:rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031120">
        <property type="term" value="P:snRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02572">
        <property type="entry name" value="PseudoU_synth_hDyskerin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21148">
        <property type="entry name" value="PUA_Cbf5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2350.10:FF:000001">
        <property type="entry name" value="H/ACA ribonucleoprotein complex subunit CBF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012960">
        <property type="entry name" value="Dyskerin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002501">
        <property type="entry name" value="PsdUridine_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004802">
        <property type="entry name" value="tRNA_PsdUridine_synth_B_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032819">
        <property type="entry name" value="TruB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00425">
        <property type="entry name" value="CBF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003280">
        <property type="entry name" value="PRK04270.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23127">
        <property type="entry name" value="CENTROMERE/MICROTUBULE BINDING PROTEIN CBF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23127:SF0">
        <property type="entry name" value="H_ACA RIBONUCLEOPROTEIN COMPLEX SUBUNIT DKC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08068">
        <property type="entry name" value="DKCLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16198">
        <property type="entry name" value="TruB_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01509">
        <property type="entry name" value="TruB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01136">
        <property type="entry name" value="DKCLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dyskerin-like" evidence="14">
        <location>
            <begin position="24"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="PUA" evidence="13">
        <location>
            <begin position="273"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="367"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="367"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00056777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00072225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00077661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00081789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00082909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAZ85114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="464" mass="51514" checksum="21B0439A73CCC60A" modified="2010-06-15" version="1">MALVHKQENEDFSIKPEAKTPTIDSSQWPLLLKNYEKLHVRTGHYTPIPNGCTPLKRELKSYISSGVINLDKPSNPSSHEVVAWVKRILRCEKTGHSGTLDPKVTGCLIVCIDRATRLVKSQQGAGKEYVCVLRLHDKIEGRAQLARALETLTGALFQRPPLISAVKRQLRIRTVHESKLLEFDNDRHLAVFWVSCEAGTYIRTLCVHLGLLLGVGAHMQELRRVRSGALSENDNIVTLHDVLDAQWLADNSRDETYLRRVIRPLESLLTTYKRIVVKDSAVNAVCYGAKLMIPGLLRYEAGIEVNEEVVLMTTKGEAIALGIAQMSTVELSTCDHGVVAKVKRCIMERDLYPRRWGLGPTAVEKKKMKSEGKLDKYGRSNEATPGEWTKKYTDYNAPAGGTEQSGSTKITEDVATAPPVPPFGAPDVEMTTPAKPESVGEKKRIVILILISSLFPFAEAKCVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKY9</accession>
    <name>D5GKY9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00009840001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430342">
        <property type="protein sequence ID" value="CAZ85182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840991.1">
        <property type="nucleotide sequence ID" value="XM_002840945.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKY9"/>
    <dbReference type="GeneID" id="9185776"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009840001"/>
    <dbReference type="eggNOG" id="ENOG502S5IN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1294525_0_0_1"/>
    <dbReference type="InParanoid" id="D5GKY9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ85182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="217" mass="24106" checksum="63364ED4D143E262" modified="2010-06-15" version="1">MAGISFTLEDKIGQIEKLAAQNKVMMMQEEDDEIVSRLMEGLQKLPPQSSLESGSNRLITAHSALATHMIHQARTLRTLTLSINYSSVPPDDDTLDLLLPLIDLIPRPAFGALTELASLHALTFSLVSQLSFLSDTLHMARQSSIAANRKLRVAREACADWKSELEQVERSRRWIEDGDWDAKCRNRNAANVCAEVTGGFEDVCRGFEERLKAQGVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GL49</accession>
    <name>D5GL49_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00009991001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430346">
        <property type="protein sequence ID" value="CAZ85242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841051.1">
        <property type="nucleotide sequence ID" value="XM_002841005.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GL49"/>
    <dbReference type="FunCoup" id="D5GL49">
        <property type="interactions" value="108"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GL49"/>
    <dbReference type="GeneID" id="9185836"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009991001"/>
    <dbReference type="eggNOG" id="ENOG502QU8E">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017709_1_1_1"/>
    <dbReference type="InParanoid" id="D5GL49"/>
    <dbReference type="OMA" id="FWFFRMR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023041">
        <property type="entry name" value="Glucose_rcpt_Git3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022596">
        <property type="entry name" value="GPR1/2/3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23112">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR 157-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23112:SF37">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR GPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11710">
        <property type="entry name" value="Git3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11970">
        <property type="entry name" value="GPR_Gpa2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="52"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="87"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="125"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="172"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="216"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="387"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="422"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose receptor Git3-like N-terminal" evidence="7">
        <location>
            <begin position="54"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="G protein-coupled receptor GPR1/2/3 C-terminal" evidence="8">
        <location>
            <begin position="377"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="264"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="325"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="517"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="557"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="265"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="284"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="524"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF11710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF11970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ85242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="596" mass="66912" checksum="2FFF1E169A56C740" modified="2010-06-15" version="1">MSSWLGMDLGKRAEGSGGEYWVDDPIYGLSRLANRKIGDPHDLSLLTGQQLYILRTIALAAATISLSTGVVVGWWFVRMKRSFRHHLIMLLVFSDFFKASWQLIYPAVVFTRGAVASESKFCQAAGFFMSMGIEASDFAVLVIAVHSALYIFRPGLTAIGEGGLFRYRHSVYVAWLAFSILMPSLAYINSAPAYSAQGTFCYLPVRPVWYRMALAWIPRYIILVTILILYASIYIYVRMKFRTFRSHVGASTLEMDTVDLAVPESPKDERGLPQLDDHGLIPTSPVSDSRQVSNASDQPFAKLMMMPQTIAEKLNFPPACSSEPRPWAYDGDEAPSRRGSLPASIANPEGGTGSSSEISHGKKVRGGTADGASAELRRRRIAISRQLRLLFIYPLVYALMWVPSLVSNLLQYRDQFVRNPNFPLACMVAFTIPIQCAVDCWLFTIREKPWRYIPESNGKSFWSRYGFIRGEGFNNIDGGGGEDGWRNRKNMSFAARKAYERREEERREAAELWLQRQDEKGRLSSGSSPSAPAEPKKTYRSWWDGFSDELDEIVEEAKNRRASKSHPGHADELSNLEAGMGEESRRDNHATPAATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLA6</accession>
    <name>D5GLA6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00010104001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Peroxisome membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430348">
        <property type="protein sequence ID" value="CAZ85299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841108.1">
        <property type="nucleotide sequence ID" value="XM_002841062.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLA6"/>
    <dbReference type="STRING" id="656061.D5GLA6"/>
    <dbReference type="GeneID" id="9182093"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010104001"/>
    <dbReference type="eggNOG" id="ENOG502S1P2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1422182_0_0_1"/>
    <dbReference type="InParanoid" id="D5GLA6"/>
    <dbReference type="OMA" id="PLCVHWS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008733">
        <property type="entry name" value="PEX11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12652:SF25">
        <property type="entry name" value="MICROBODY (PEROXISOME) PROLIFERATION PROTEIN PEROXIN 11C (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12652">
        <property type="entry name" value="PEROXISOMAL BIOGENESIS FACTOR 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05648">
        <property type="entry name" value="PEX11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="1" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00046271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ85299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="209" mass="23323" checksum="6260681AA1019F3F" modified="2010-06-15" version="1">MAPKSDTMQKFNAFMIRLNRLLSSPSGIDKVLMTTHYTLVLLPPSVLSSTRSTRINIPKLTSLISDVRMFMRLWGLIGIYTWGLSTLSASRPSPIEIAQVFANTCFQICENVAYLSSHGIVRIRKRTEGQLWLWSSRFWMAHVALEFVRLFGGNRRGKGWEREVLSNCAYAPLTVHYSVEGGAISPEAIAACGSAAAIIGLQNEWRKTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLB9</accession>
    <name>D5GLB9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00010124001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430348">
        <property type="protein sequence ID" value="CAZ85312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841121.1">
        <property type="nucleotide sequence ID" value="XM_002841075.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLB9"/>
    <dbReference type="STRING" id="656061.D5GLB9"/>
    <dbReference type="GeneID" id="9182106"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010124001"/>
    <dbReference type="eggNOG" id="KOG2896">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009375_1_0_1"/>
    <dbReference type="InParanoid" id="D5GLB9"/>
    <dbReference type="OMA" id="HYRFEYG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000323">
        <property type="term" value="C:lytic vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034272">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035493">
        <property type="term" value="P:SNARE complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040939">
        <property type="entry name" value="Vps38"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15157">
        <property type="entry name" value="UV RADIATION RESISTANCE-ASSOCIATED GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15157:SF5">
        <property type="entry name" value="UV RADIATION RESISTANCE-ASSOCIATED GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17649">
        <property type="entry name" value="VPS38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="298"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ85312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="596" mass="66312" checksum="F31DCAA5D5332B8E" modified="2010-06-15" version="1">MPSDSDGLRTLNIDFALGIGTTPRECPLLLPSGRKLRHLTGIALRNLTTSVQKAQSTRVTKSSDDDALAGAWKSAGKRPVQVEGLAGGSSSSSGGGLIHAKSEMDLTKADVDMEVGVDVRSGGRLRRRSMRGNPLGLESPMTRQKRLEDVAAGRMADVFFSLHVGDRVGSGEDPIYISEVVSKSMNPSFQFFELSHSGPHVSRLDKVTVKIWVGTQSQFRLILELNIALSALQFIGKNLENFRHPFPTNSVIFFLSDGIYTTFMDMPLSSPASHRELPIRGHASSASPTASYDAIMKLNNLEDCIQDAEQTSAKVSDQINAILERESSSMSLLREASQARESRKTLQGYLALEKKRLNAAIRKRESLRQSLQVRREAMAAGREAQRTGENYLREAAVGLERCKKDLGETKKGIETQRRRIVEDLQTVYPVEPVPNHPLSFTIRSLPLPNSHHEDADADAISAALGYVAHVAYLLSFYLGTYLRYPLQPQCSNSFVRDPISVIAGARTFPLWVKGSVYFRFEYAIFLLNKDLEQLMSSQGLWVMDIRHTLPNLKYLLLFRWGGGGYAGTNKALITLRKITLPEVLRDWLSFRCYSYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLN2</accession>
    <name>D5GLN2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00010281001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430350">
        <property type="protein sequence ID" value="CAZ85425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841234.1">
        <property type="nucleotide sequence ID" value="XM_002841188.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLN2"/>
    <dbReference type="STRING" id="656061.D5GLN2"/>
    <dbReference type="GeneID" id="9182752"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010281001"/>
    <dbReference type="eggNOG" id="KOG0676">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023246_0_0_1"/>
    <dbReference type="InParanoid" id="D5GLN2"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="333" mass="36336" checksum="BFAB56160C34A84C" modified="2010-06-15" version="1">MGVLSKVVKSLFVHFQMPAVMLMQEPVMAVVGAGVRSGVVLDVGWNEAVINVVYEMRVVGPGKRSRRGGRTYRDFWRKFLQEEYSSSSSDEDNGNGTSEDNEIPVADWEVEEILTRASWCQSSSPSSRHQDEITITIPLTTPTPPKTLTFPFNKLATPAEKTFFATTTTDSPTPEYPDDDDYALPELVYRALRDCPIDVRAAVMQRIIITGGGSYVPGLKKRVIDEVRVLVKCRGWERVGRRAVKTGKGKMPAGKEGVEEGYQWRREATASPEGQKGGGDTGGRGRIAGVESFGSWVGASLVAGMKVRGTVEVEREKFLAAVAAGGTGFPLDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLR0</accession>
    <name>D5GLR0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00010364001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430351">
        <property type="protein sequence ID" value="CAZ85477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841286.1">
        <property type="nucleotide sequence ID" value="XM_002841240.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLR0"/>
    <dbReference type="GeneID" id="9186461"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010364001"/>
    <dbReference type="eggNOG" id="ENOG502S09C">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028200_0_4_1"/>
    <dbReference type="InParanoid" id="D5GLR0"/>
    <dbReference type="OMA" id="GANILIW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF123">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="83"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="120"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="8">
        <location>
            <begin position="5"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="222"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="230"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ85477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="282" mass="31073" checksum="D80365AAF78A1CBD" modified="2010-06-15" version="1">MLKLWYVYQLQYLVTLFFIKASILSFYRRLSRERTYQISIWIVSGIVVVFTLAMLFVNVFECPKNPSKAWSPGFPAGCNDLVVVYYIMASFNILSDIVILILPLPTLVRLQTNRRKRAALILIFSCGSIAVVGSVVRITALYKNQHAMDTGGDVSYVATYVLLWSQVEVNFGIITASAPALKPLVHSILQGTSHAKPSYAQNGLGYTAGGRKSGAGVPLHSLGCRDTNRPHGNTTTVDGGSSESQEDIVFKGDGIVRTVEVKVDMEADGRSEDSIRRLEFGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLS6</accession>
    <name>D5GLS6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00010395001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430351">
        <property type="protein sequence ID" value="CAZ85493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841302.1">
        <property type="nucleotide sequence ID" value="XM_002841256.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLS6"/>
    <dbReference type="GeneID" id="9186477"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010395001"/>
    <dbReference type="HOGENOM" id="CLU_1094953_0_0_1"/>
    <dbReference type="InParanoid" id="D5GLS6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="14"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="144"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="219"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="236"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="254" mass="28207" checksum="05DB74BDE9F2BA15" modified="2010-06-15" version="1">MCFCGPEEYPYEERSVPRVVSMSEKSSSSYGRYAQPSSHYTLSSGRPSKRQSVRIAEHDPHPYDSGYESVGASLRRESFTPPPVLIQTRPMGCIDTPNRPSSVAPGGGRRPFGARSGEMGYNQPRILFDGGLGPIVHQGHSAAGFQIPHHTGHPAHMHSSGPRASIPRQFSISANPQRGRSGRYVGYRDERNGWREERGYSRDDYRDDRGSSSSEDSWASDRRLFPGSPDKRSSYAAPSSTESFYTSPSLRRFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLV8</accession>
    <name>D5GLV8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 11">13 kDa ribonucleoprotein-associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00010439001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 11">Common component of the spliceosome and rRNA processing machinery. In association with the spliceosomal U4/U6.U5 tri-snRNP particle, required for splicing of pre-mRNA. In association with box C/D snoRNPs, required for processing of pre-ribosomal RNA (rRNA) and site-specific 2'-O-methylation of substrate RNAs. Essential for the accumulation and stability of U4 snRNA, U6 snRNA, and box C/D snoRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the U3 snoRNP particle. Binds to the C'/D and B/C motifs in U3 snoRNA. Component of the 25S U4/U6.U5 tri-snRNP particle, a subcomplex of the spliceosome. Binds to the 5' stem-loop of U4 snRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
            <location evidence="1 11">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the eukaryotic ribosomal protein eL8 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430351">
        <property type="protein sequence ID" value="CAZ85525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841334.1">
        <property type="nucleotide sequence ID" value="XM_002841288.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLV8"/>
    <dbReference type="FunCoup" id="D5GLV8">
        <property type="interactions" value="1160"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GLV8"/>
    <dbReference type="GeneID" id="9186509"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010439001"/>
    <dbReference type="eggNOG" id="KOG3387">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084513_4_1_1"/>
    <dbReference type="InParanoid" id="D5GLV8"/>
    <dbReference type="OMA" id="IKNQIYA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031428">
        <property type="term" value="C:box C/D methylation guide snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030621">
        <property type="term" value="F:U4 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000494">
        <property type="term" value="P:box C/D sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000452">
        <property type="term" value="P:snoRNA guided rRNA 2'-O-methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd21104">
        <property type="entry name" value="SNU13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000002">
        <property type="entry name" value="NHP2-like protein 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002415">
        <property type="entry name" value="H/ACA_rnp_Nhp2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004037">
        <property type="entry name" value="Ribosomal_eL8-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018492">
        <property type="entry name" value="Ribosomal_eL8/Nhp2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00881">
        <property type="entry name" value="L7ARS6FAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00883">
        <property type="entry name" value="NUCLEARHMG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01082">
        <property type="entry name" value="RIBOSOMAL_L7AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="12">
        <location>
            <begin position="19"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU366039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ85525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="126" mass="13710" checksum="5FED519EEB45A48E" modified="2010-06-15" version="1">MASVNPKAFPLADEALTQKILDIVQQASHYRQLKKGANEATKTLNRGISEFIVLAADTAPLEILLHLPLLCEDKNVPYVFVPSKIALGRACGVSRSVISASVTSNEASELQGQIRSVKEKIERLLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMC9</accession>
    <name>D5GMC9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00010654001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430354">
        <property type="protein sequence ID" value="CAZ85672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841481.1">
        <property type="nucleotide sequence ID" value="XM_002841435.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMC9"/>
    <dbReference type="STRING" id="656061.D5GMC9"/>
    <dbReference type="GeneID" id="9183242"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010654001"/>
    <dbReference type="eggNOG" id="KOG1963">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005417_0_1_1"/>
    <dbReference type="InParanoid" id="D5GMC9"/>
    <dbReference type="OMA" id="TRIDGPH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000234">
        <property type="term" value="P:positive regulation of rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045943">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053826">
        <property type="entry name" value="WDR75"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44215">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 75"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44215:SF1">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 75"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23869">
        <property type="entry name" value="Beta-prop_WDR75_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69322">
        <property type="entry name" value="Tricorn protease domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="411"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="355"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1012"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="49"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="366"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="1031"/>
            <end position="1043"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ85672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1043" mass="114564" checksum="4A4C3DEAB94C505B" modified="2010-06-15" version="1">MSSARAPGAVKEIEKPSGPMGKKISEISTQEPDANPDALATLTEGGAERKRRRGRDRTRRRREDRHAIREGLAETEGARLGEVKEILKEDRIVRVAVSRQPNELQTPKKKDSGESKAAAYNTVASAEGNGGSGVSGWRMSSALGGRFLPLDMVFSQDEKFFLLAHSCSLRIYSTKTSLQVREFNLDGKPSEVITSFKLDPLDSSKVYIGTSASKIFFVDWTTGKLVKSWQLNNIRTIQRLAICMDEKREDDTTYGILYVSGPRSKNSSIMIQRIKLSPSVAPIEDMVYVAPKPSPIGCMQVLDQGNIVTAIAGNALIIGNRNTEEGSSDKKPWGVFRHYPMSFHLACMDTYLHSEPDTKKHKGGRSNRESGREADRSGDVVVGDNQGALHLFHNVLRVKGLPKESPVISKMHWHRHRVQAVKWALDGKYIISGGSETVLVIWQLGTGSKQFLPHLGATIHNITISPKGSSYGVGFADNSVMVLSTTEFKPTSSVTGIQSRVFHLEKGTEYPKFTCVMHPTLPNRLLLASPSSQTDLYSSASSLQTFDISSNRQIHRQALTKNNITSVNQGPDEYKIKEPDLPHLAITKDGKWLASIDEWKPPKKRSFCSSEDGRSSQEGDRDQSEVSLRFWKWSEKSLSWELATIIKSPHPSSSGTGPEEVLDLVAAPKTHLFATLAGDGSIRLWKPKVRASDGARLRGEDSEITVWSCRKVVKFSKGKREQQVLAQNLLGGVSLQKAWWGSAAFSEDNSVIAAAVPNSLGDDSAIYLTDPSSGTIAHFLSGLRVGKTAGIAILERFLIILGTSKLLVWNMVNGTVQWEFNLKTLSSVDSPVTHLAVDYVSQTFAITASQRDAQSPKIFIFEAPHPTPVHIQNFEYGYQIMALIATDTGKGYVTLDNQARVRCLSPILAGPVQRIPAEDGEEESLLVAEEDEPKIGTLSGAYLSTTAHTTSAEDRMDLDCVKNDGVVDHVVSREALEDILDSTPSYAIGSLDYIFDQVLQLFAKAPLLNDGDDRYDCEGVEEGGLDNGSGNEEDDNEEDAMEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMG7</accession>
    <name>D5GMG7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00010699001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430354">
        <property type="protein sequence ID" value="CAZ85710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMG7"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010699001"/>
    <dbReference type="HOGENOM" id="CLU_3423352_0_0_1"/>
    <dbReference type="InParanoid" id="D5GMG7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ85710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="23" mass="2492" checksum="1141B10CBF508B00" modified="2010-06-15" version="1">MKGIALGLCQGEDGNIGFQKKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMX8</accession>
    <name>D5GMX8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00010987001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the group II decarboxylase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430360">
        <property type="protein sequence ID" value="CAZ85871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841680.1">
        <property type="nucleotide sequence ID" value="XM_002841634.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMX8"/>
    <dbReference type="STRING" id="656061.D5GMX8"/>
    <dbReference type="GeneID" id="9183439"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010987001"/>
    <dbReference type="eggNOG" id="KOG0628">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011856_3_1_1"/>
    <dbReference type="InParanoid" id="D5GMX8"/>
    <dbReference type="OMA" id="NPGFNWS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1340.10">
        <property type="entry name" value="dopa decarboxylase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010977">
        <property type="entry name" value="Aromatic_deC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021115">
        <property type="entry name" value="Pyridoxal-P_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11999">
        <property type="entry name" value="GROUP II PYRIDOXAL-5-PHOSPHATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11999:SF70">
        <property type="entry name" value="MIP05841P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00800">
        <property type="entry name" value="YHDCRBOXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00392">
        <property type="entry name" value="DDC_GAD_HDC_YDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="5 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 6 7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="6">
        <location>
            <position position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ85871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="532" mass="58611" checksum="7E37B37681529666" modified="2010-06-15" version="1">MDSEQFRAAAYSAIDQIIDYYDTIESRRVVSNVSPGYLKPLLPSGPPANPEPWADIQSDIESKILPGITHWQSPNFLAFFPANSSYPGILGELYSATFNSANFNWLCSPAATELETIVLDWLARLLNLPECFLSTSRNGGGGVIQGSASEAICTVIVAARERYLGNLAAAGKSVEQSEGIIDNNRSKLVALFSDQTHSSTQKGCQIAGVKHHSIQVPGSATENYVLTGPLLRKALEELTAKGLHPFFLTVTLGTTATCAADDFASIVPVLKDYPNLWVHVDAAFAGAALILPQYHHVPAPFKHFDSFNMNMHKWLLTNFDCSCLYVRTRKHLLDALSIAPPYLRNPFSEQGLVTDYRDWQIPLGRRFRALKIWFVMRSYGVSGLQRHIWDSIRHGESFAQWVRDRADIFEIVVPPAFALTVFAIKTTRREESNRLTRVVYEAVNADGEIFITSTVIDGIYAIRVVGGGPKIREEVLRRAFEILVSKTEEVLKAEGTPQVSGINGINGVVKIDCVNGVDDIKLGGVDGIATLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>D5GN39</accession>
    <name>D5GN39_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00011075001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430362">
        <property type="protein sequence ID" value="CAZ85932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841741.1">
        <property type="nucleotide sequence ID" value="XM_002841695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GN39"/>
    <dbReference type="STRING" id="656061.D5GN39"/>
    <dbReference type="GeneID" id="9185858"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011075001"/>
    <dbReference type="eggNOG" id="ENOG502S6GP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1066300_0_0_1"/>
    <dbReference type="InParanoid" id="D5GN39"/>
    <dbReference type="OMA" id="QRMMHVT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056004">
        <property type="entry name" value="DUF7582"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24483">
        <property type="entry name" value="DUF7582"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7582" evidence="1">
        <location>
            <begin position="4"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="261" mass="30048" checksum="F7FC17477A8CE513" modified="2010-06-15" version="1">MEAHTVIKALEYISQKLCRKGIHVHLIVAGDALSCLLFKSRPTTHSISILQASALSPRSLSVLFGAVHRAEKKFGLANDWVNTDLGRQVDSDYLDYVVHKSLLQNEIVFSSEGLTLVAIDLCFALKSKLESLTNKFTQKDIEDAVVLLRRLVVIYRGRPLTKGYIRMSYSRMEVSDQILLRLNELYEWQYSTRGVAGVHDEYMEWRREGIADWSFGFQDLKREASVHAPLEKGLYSWRDKPLPDTPYREDFAEIFTQQRVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNA7</accession>
    <name>D5GNA7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00011201001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TDE1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430363">
        <property type="protein sequence ID" value="CAZ86000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841809.1">
        <property type="nucleotide sequence ID" value="XM_002841763.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNA7"/>
    <dbReference type="FunCoup" id="D5GNA7">
        <property type="interactions" value="501"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GNA7"/>
    <dbReference type="GeneID" id="9182242"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011201001"/>
    <dbReference type="eggNOG" id="KOG2592">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029574_2_0_1"/>
    <dbReference type="InParanoid" id="D5GNA7"/>
    <dbReference type="OMA" id="DKHCNPL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005016">
        <property type="entry name" value="TDE1/TMS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10383">
        <property type="entry name" value="SERINE INCORPORATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10383:SF9">
        <property type="entry name" value="SERINE INCORPORATOR, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03348">
        <property type="entry name" value="Serinc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072223" evidence="8">
        <location>
            <begin position="23"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="49"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="101"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="164"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="203"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="233"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="266"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="305"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="414"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="453"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="329"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="377"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="337"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="385"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ86000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="481" mass="51507" checksum="A8EDE7D765F2DD4C" modified="2010-06-15" version="1">MGALLSIPFLAVPSLTTIGSFAASCCGAAACSALCSACGKCGSSTATRIAYALLFLLNSIISWIMLSPWAIKKLEHLTLDYFPITCLGEQCYGFVAVHRIQFALGVFHAVLAAILVGVKSSKGGRAAIQNGYWGPKIIAWLLLIVLTFLVPEGFFLVWGNYVATFGAVLFLLLGLVLLVDLAHTWAEVCLEKIDKSDSRIWRGILLGSTLGMYIGSLVLTIVMYVFFAGSGCSMNQAAITINLILLILVSAISVHPKVQEYNSQAGLAQSAMVAIYCTYLTMSAVSMEPDDKQCNPLLRARGTRTASIVLGAIVTLLTIAYTTTRAASQGVGPLHRGSSSPNNGGYSSLGDGEHGLVSTEPSRSELRARALRRAVEDGSLPASALDDDGDSDDEDDARANDDEKNGAQYSYSGFHIIFFLATAWTATLLTMSLEPGKNDEEGFTPVGRTYAASWIKIVSAWVCYALYSWTLVAPVVLPERF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNI3</accession>
    <name>D5GNI3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00011302001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430365">
        <property type="protein sequence ID" value="CAZ86076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841885.1">
        <property type="nucleotide sequence ID" value="XM_002841839.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNI3"/>
    <dbReference type="GeneID" id="9186152"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011302001"/>
    <dbReference type="HOGENOM" id="CLU_1046593_0_0_1"/>
    <dbReference type="InParanoid" id="D5GNI3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="14"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="136"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="184"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="208"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ86076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="266" mass="28436" checksum="B8C0CFCAA6688E65" modified="2010-06-15" version="1">MGWTFYPVCSASTIPVERPESEEAEEQPLIDLDSDPQVNDTNPAPSRSLGFFSDIGGELLDIPPLQTPPPLDEDTTEDLPATPEPDGSSLPSPQRFPDSNLLQVVSEAPQMEHIDEIQTQEPLPDSQDDAQATSIHGEDPPKSIVEDAAQGIDSEDTDETTVEPPSNEASNYQIPITIVEVIDKPPPPPPPPPPPPPPPSTTPIEENHCDEDEAVDDSDGDGDSDIFEDAPQTPSDIVDILGGQETGKSKDQHLGAHTPLVISSSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNL6</accession>
    <name>D5GNL6_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable kinetochore protein NUF2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">GSTUM_00011355001</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
            <location evidence="3">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the NUF2 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430366">
        <property type="protein sequence ID" value="CAZ86109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841918.1">
        <property type="nucleotide sequence ID" value="XM_002841872.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNL6"/>
    <dbReference type="FunCoup" id="D5GNL6">
        <property type="interactions" value="253"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GNL6"/>
    <dbReference type="GeneID" id="9186185"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011355001"/>
    <dbReference type="eggNOG" id="KOG4438">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025461_1_1_1"/>
    <dbReference type="InParanoid" id="D5GNL6"/>
    <dbReference type="OMA" id="YLKMEAH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035974">
        <property type="term" value="C:meiotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031262">
        <property type="term" value="C:Ndc80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140483">
        <property type="term" value="F:kinetochore adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051315">
        <property type="term" value="P:attachment of mitotic spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140456">
        <property type="term" value="P:initial meiotic spindle pole body separation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000073">
        <property type="term" value="P:initial mitotic spindle pole body separation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051383">
        <property type="term" value="P:kinetochore organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990571">
        <property type="term" value="P:meiotic centromere clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.60">
        <property type="entry name" value="Ncd80 complex, Nuf2 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005549">
        <property type="entry name" value="Kinetochore_Nuf2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041112">
        <property type="entry name" value="Nuf2_DHR10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038275">
        <property type="entry name" value="Nuf2_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21650:SF2">
        <property type="entry name" value="KINETOCHORE PROTEIN NUF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21650">
        <property type="entry name" value="MEMBRALIN/KINETOCHORE PROTEIN NUF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03800">
        <property type="entry name" value="Nuf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18595">
        <property type="entry name" value="Nuf2_DHR10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="13" id="KW-0137">Centromere</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="10 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="8" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinetochore protein Nuf2 N-terminal" evidence="15">
        <location>
            <begin position="16"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Nuf2 DHR10-like" evidence="16">
        <location>
            <begin position="264"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="150"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="304"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF03800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF18595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAZ86109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="445" mass="51563" checksum="EB101E9B7F91F986" modified="2010-06-15" version="1">MKGKQQQQQYAADNHLLLKDNELVDCISELGIPFQMEDLKKPSPQKIQLVFEMFADLLMGVRKETVEPVLNAAAQQVLEFPDTQTDSHTLMAFYVSLSQLMLECGIEDFTFNDLAKPDSTRLTRILSYVINFTRFREERAGVIDEHFGKAQKAKEKIEQLYFENEDLNNRLQELKMQRLKEEPMINKAKKVLSALVADLETVRKRQGALTNDLDRLKQLKVSHVQTLEDRQYLKVKTQQENNKIRPYIVDSPQKLQQVITDMANSLGAEKAAHDSLERKSRSLQTSADSFVIVEQDVAGCVKVMEEVEQELVKEEETSRRASRHTEILTVKQTEVHEVERSEKRLSRQLENATEKLRRARETGEGKAEAAQKRMLELKEAYGVLSRERSERDKEMERKKIRIERIEKEMADMKETVEAEVAAAHKEFAKMKSHVELYMSEMEQCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNV6</accession>
    <name>D5GNV6_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">chitinase</fullName>
            <ecNumber evidence="2">3.2.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00011529001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.14"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the glycosyl hydrolase 18 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.14" evidence="2"/>
    <dbReference type="EMBL" id="FN430370">
        <property type="protein sequence ID" value="CAZ86199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842008.1">
        <property type="nucleotide sequence ID" value="XM_002841962.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNV6"/>
    <dbReference type="FunCoup" id="D5GNV6">
        <property type="interactions" value="143"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GNV6"/>
    <dbReference type="GeneID" id="9182833"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011529001"/>
    <dbReference type="eggNOG" id="KOG4701">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007818_1_0_1"/>
    <dbReference type="InParanoid" id="D5GNV6"/>
    <dbReference type="OMA" id="FYNNGNC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008843">
        <property type="term" value="F:endochitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001579">
        <property type="entry name" value="Glyco_hydro_18_chit_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050542">
        <property type="entry name" value="Glycosyl_Hydrlase18_Chitinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45708">
        <property type="entry name" value="ENDOCHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45708:SF49">
        <property type="entry name" value="ENDOCHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01095">
        <property type="entry name" value="GH18_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0146">Chitin degradation</keyword>
    <keyword evidence="6 8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072858" description="chitinase" evidence="10">
        <location>
            <begin position="25"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="GH18" evidence="11">
        <location>
            <begin position="52"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ86199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="340" mass="35937" checksum="C778F69583DB130A" modified="2010-06-15" version="1">MTSFLLKTLLTLLLAILGLSSAAALPMGTLRARAVGFPINTALPTGKSTTPAQTVAYFGQFGKLGEDSLSKFCSNTNVDVVVLAFVNVYKGAGGLPGMNLGVYCDTRIPGTDLLSCPNIGKDIAACQAAGKKVLLSLGGQEANGNSLPDDNSATELGDNLWKLFGEGKGLESKRPFGSALIDGFDIDNENKDSQGYPKLISTLRANFKTGTKKYYISAAPQCPMPDESLDEAVYKVDYLFIQHYNNPACQLGNFVNSFKAWSANIAARTTCGTKVFAGFPGSETAAGQGYATHDDMKKYVAAVRGLPNFGGVMVWDGVQAAGNVVSGKSFLQAMREALAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNW8</accession>
    <name>D5GNW8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">LSM2-LSM8 complex subunit LSM8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LSM8</name>
        <name evidence="11" type="ORF">GSTUM_00011556001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Plays role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex). The heptameric LSM2-8 complex binds specifically to the 3'-terminal U-tract of U6 snRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">LSm subunits form a heteromer with a doughnut shape.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430371">
        <property type="protein sequence ID" value="CAZ86211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842020.1">
        <property type="nucleotide sequence ID" value="XM_002841974.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNW8"/>
    <dbReference type="FunCoup" id="D5GNW8">
        <property type="interactions" value="383"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GNW8"/>
    <dbReference type="GeneID" id="9186514"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011556001"/>
    <dbReference type="eggNOG" id="KOG1784">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076902_8_1_1"/>
    <dbReference type="InParanoid" id="D5GNW8"/>
    <dbReference type="OMA" id="AACDQTT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005688">
        <property type="term" value="C:U6 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030620">
        <property type="term" value="F:U2 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01727">
        <property type="entry name" value="LSm8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000027">
        <property type="entry name" value="U6 snRNA-associated Sm-like protein LSm8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034103">
        <property type="entry name" value="Lsm8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044642">
        <property type="entry name" value="PTHR15588"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15588">
        <property type="entry name" value="LSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15588:SF9">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 9" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="10">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ86211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="99" mass="10781" checksum="05033E89538AEF7E" modified="2010-06-15" version="1">MSNLQTFVNKTVTIITSDGRTLTGKLMGHDQTTNLILSRTIERIFATPGSEEATQQVEHGLYLIRGDNVAICGLVDEQLEAGIDWSAVRAEPLGGMKHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNX8</accession>
    <name>D5GNX8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">asparagine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Asparaginyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00011574001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>tRNA(Asn) + L-asparagine + ATP = L-asparaginyl-tRNA(Asn) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11180"/>
            <dbReference type="Rhea" id="RHEA-COMP:9659"/>
            <dbReference type="Rhea" id="RHEA-COMP:9674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78515"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.22"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.22" evidence="3"/>
    <dbReference type="EMBL" id="FN430371">
        <property type="protein sequence ID" value="CAZ86221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842030.1">
        <property type="nucleotide sequence ID" value="XM_002841984.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNX8"/>
    <dbReference type="FunCoup" id="D5GNX8">
        <property type="interactions" value="872"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GNX8"/>
    <dbReference type="GeneID" id="9183514"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011574001"/>
    <dbReference type="eggNOG" id="KOG0555">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004553_2_10_1"/>
    <dbReference type="InParanoid" id="D5GNX8"/>
    <dbReference type="OMA" id="DCCLYPR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004816">
        <property type="term" value="F:asparagine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006421">
        <property type="term" value="P:asparaginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04323">
        <property type="entry name" value="AsnRS_cyto_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00776">
        <property type="entry name" value="AsxRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000040">
        <property type="entry name" value="Asparagine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1910.20">
        <property type="entry name" value="asparaginyl-tRNA synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004522">
        <property type="entry name" value="Asn-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048952">
        <property type="entry name" value="AsnRS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00457">
        <property type="entry name" value="asnS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594:SF16">
        <property type="entry name" value="ASPARAGINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594">
        <property type="entry name" value="ASPARTYL/LYSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20917">
        <property type="entry name" value="AsnRS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="12">
        <location>
            <begin position="276"/>
            <end position="571"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ86221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="579" mass="64632" checksum="0663215D96621653" modified="2010-06-15" version="1">MAEEAKTLATSAANLSVSEPDNTLFIDEDIGVDAAETPGTEGQPFKTLIGAMTHTYKSQPDAIFKIRKKPTGPEDKTPHLYAPVAKAALKKAANLHQANLKKEAKRAEMSIKEQEAKAARGAQLEQAKKVIIVEDASKPKAISIKLRDTVKRREARVVVRGWVHRLRVQKSLVFITLRDGTGYLQCLLSGDLTKTYDALTLTVETTIAIYGVISPVPEENHAPDGHELHADYFQVIGKAPGGDDTISNIVAPNADPQTKYDNRHLVIRGDVASSVLKVRASVLRAFRKSYEDLGLLEVTPPCMVQTQVEGGSTLFEFNYYGEKAYLTQSSQLYLETCLPSLGDVFCVQESFRAERSLTRRHLSEYTHIEAELAFITFDDLLNHLEEVICRTINYALTDPLIKGKVAELNPKFEAPERPFMRMTYADAIGWLVENNIPNEEGNPHKFGDDIAEAAERKMTDIIAKPIFLTKFPVKIKAFYMKKDPADPRVTESVDCLMPGVGEIVGGSMRMENLEELMGAYAREGISPDPYYWFTDQRKYGTSPHGGYGIGLERLLAWMCDRYTVKECSLYPRWTGRCTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNY9</accession>
    <name>D5GNY9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00011590001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430371">
        <property type="protein sequence ID" value="CAZ86232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842041.1">
        <property type="nucleotide sequence ID" value="XM_002841995.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNY9"/>
    <dbReference type="GeneID" id="9183525"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011590001"/>
    <dbReference type="eggNOG" id="ENOG502S5UU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078844_1_0_1"/>
    <dbReference type="InParanoid" id="D5GNY9"/>
    <dbReference type="OMA" id="GIWNIKD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="16"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ86232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="178" mass="19463" checksum="056C15A0291CE501" modified="2010-06-15" version="1">MAATKSKSDVPPENQAPSSSKAAAKGSHRRGSSSVTDVYKPAELKDIIGEEGTLMVAKEISRLNWKLNTHPSHLDDWKSLKEIPITTPKVKLLDVYFPLGLHVTARAGSSSTVGVSVYDVLVAIHKQFKKKADDELEMPVLGNLEWGRSEDLSSKEHWPAVVLVAQKKEAPVTGKKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GP56</accession>
    <name>D5GP56_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00011710001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF11 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430373">
        <property type="protein sequence ID" value="CAZ86321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842130.1">
        <property type="nucleotide sequence ID" value="XM_002842084.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GP56"/>
    <dbReference type="STRING" id="656061.D5GP56"/>
    <dbReference type="GeneID" id="9186561"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011710001"/>
    <dbReference type="eggNOG" id="KOG3219">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_963182_0_0_1"/>
    <dbReference type="InParanoid" id="D5GP56"/>
    <dbReference type="OMA" id="ICGFSKV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08048">
        <property type="entry name" value="HFD_TAF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045127">
        <property type="entry name" value="TAF11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006809">
        <property type="entry name" value="TAFII28_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13218:SF8">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13218">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04719">
        <property type="entry name" value="TAFII28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TAFII28-like protein" evidence="7">
        <location>
            <begin position="110"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="72"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ86321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="258" mass="27202" checksum="FF2C3C7CABD0CB81" modified="2010-06-15" version="1">MSSPPSVSSPPFYPPGSHKRRISITGSSLPPPAAKKPKIPAPRKLSTFAQPSLHPLRQTSFPPDPPPPGAKEDEEDDDAREDDYGTSTVLAEGAAGNFGGEDDEKIKLSVLLDSFDSQQTQRYEAFRRANLNKAAVKKLANQVLSQSVTAGVGTAICGFSKVFTGEIVELALKVQKEWADADVEGEGIENIRKRTKSDGSLGPLLPDHLREAARRYRLELRGAVGFRGSIGGGCGNALGAGPGAGGEGAIGGVGRLFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5GP82</accession>
    <name>D5GP82_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00011747001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430373">
        <property type="protein sequence ID" value="CAZ86347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842156.1">
        <property type="nucleotide sequence ID" value="XM_002842110.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GP82"/>
    <dbReference type="GeneID" id="9186587"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011747001"/>
    <dbReference type="eggNOG" id="ENOG502R87S">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006056_0_0_1"/>
    <dbReference type="InParanoid" id="D5GP82"/>
    <dbReference type="OMA" id="GMKRAYD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013244">
        <property type="entry name" value="Sec39_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40787:SF3">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40787">
        <property type="entry name" value="SECRETED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08314">
        <property type="entry name" value="Sec39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Sec39" evidence="6">
        <location>
            <begin position="18"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="701"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="706"/>
            <end position="722"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ86347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="805" mass="87655" checksum="D1CF089B12D52A1E" modified="2010-06-15" version="1">MPKPNPVSASELSAAKSIVLAAHCASSAELKSLRSLFFLHPSTFSKEMQLRVLLCLPETTKPDLYVPLIKGEPGESATEVDTAAVSKISEKTANKRVKELPTLTLLESERTSDEGMLLSYLVCRAWNIDRETGALSIVIELLSPFVGVVMGIDKYLEGIVEVLARLTYDYASEDVGTGEEAEDGGGRLETFGELEAQVGIRLLLARCGGRQVVDDLKRLVGPYLGAKRNDREGWEVVWEWLKCKIADGNWGGFVEVVTKWDGPVGDRELAEEYARISIAGCYVCLETEQLVLEGMRAIHRRCSKYVSEDQGFKDQKLPGKLGDPFDKGNPLTAPNETSLQLLDVLITSAGVLSTPLANVAGIRLEGSYADQEALLVRHVRNGPNWAKQSDEEWRRTRDGVKWLQTKAKVFDKLTVEEVEKVLLSAMLAATKFDLVKEIYVNGRNGSLRKGDVETGILDAFQGFYDNASNGNKTRGSMKNALGTLQILYPRTSKSTALERAYRLTNATHALSHYPLALAPGIPLLPVQIRIHSDPVSLVARLLDSTPKSFAQSETLVTIARDLRFGTTGNDGGKKTDARVIGMCIEAALSEGDFETAHSLGMNRLVIMIAGNERNVDEDVADMVWRACFQAGRYRSPYAALVGETAAFGGKALRTVEMRMELLAQALRFCPPSALAEVLASWRRCEEEMLIGLKEEEEEEKREAYWSSNNNSGGNTSTISSASRGEDDETPMGLFAVAAGAAKALRGTAFPLSTPRGNPAPIGDGAAAAMEEQRTRKRDMVSGMVTSGLASGLGWVLGAQPNVNRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5GP91</accession>
    <name>D5GP91_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00011757001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430373">
        <property type="protein sequence ID" value="CAZ86356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842165.1">
        <property type="nucleotide sequence ID" value="XM_002842119.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GP91"/>
    <dbReference type="GeneID" id="9186596"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011757001"/>
    <dbReference type="HOGENOM" id="CLU_947296_0_0_1"/>
    <dbReference type="InParanoid" id="D5GP91"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ86356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="294" mass="32529" checksum="A5FD04E57ADEB894" modified="2010-06-15" version="1">MSTTIPSTTTIASLPVEIHAQILRSLPCFRDVHDAILASRLFHNAWVSGQNGIINAIARETIQPLEPLVRLYLAREQQKFEVSVDGATNHRLTLQTITNIREYIKAAATQYNAMVSTPLLRRNYRIGEGPEPPPRTPCALELDLFTMTYLTMKLIEAYPPNLIAPLLSRTATMDLLRIRQLQKHMYARGPPEDDYGGDRGGLVQREFMARLPRGSGIWEAMAGRMIGGEPFALWREGQEQVRGLERAMKEGKFPWDAGASGAVEVVRGSGGVNGFTGVQTAGGCSGIIGFYRYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5GP94</accession>
    <name>D5GP94_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">Vacuolar protein sorting-associated protein 29</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00011762001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the VPS29 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430373">
        <property type="protein sequence ID" value="CAZ86359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842168.1">
        <property type="nucleotide sequence ID" value="XM_002842122.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GP94"/>
    <dbReference type="FunCoup" id="D5GP94">
        <property type="interactions" value="1026"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GP94"/>
    <dbReference type="GeneID" id="9186599"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011762001"/>
    <dbReference type="eggNOG" id="KOG3325">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063749_0_1_1"/>
    <dbReference type="InParanoid" id="D5GP94"/>
    <dbReference type="OMA" id="VRGNMDY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030904">
        <property type="term" value="C:retromer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07394">
        <property type="entry name" value="MPP_Vps29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000024">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024654">
        <property type="entry name" value="Calcineurin-like_PHP_lpxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000979">
        <property type="entry name" value="Phosphodiesterase_MJ0936/Vps29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028661">
        <property type="entry name" value="Vps29"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00040">
        <property type="entry name" value="yfcE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11124">
        <property type="entry name" value="VACUOLAR SORTING PROTEIN VPS29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12850">
        <property type="entry name" value="Metallophos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="6">
        <location>
            <begin position="6"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ86359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="200" mass="21867" checksum="01F84C364AB86FF7" modified="2010-06-15" version="1">MASRLVLCIGDLHIPDRAPDIPAKFRKLLSPGKIGQILCLGNLTDKETYDFLRSVAPELQIVKGDFDTEAANLPLSKVVTHGNLRIGFTHGHTIVPQGDSDSLLIEARRLDCDVLVWGGTHRFEAYELEGRFFINPGSATGAISGGWVSTDEPIVPSFCLMDVQGSVLVLYVYQLRTDEKGVESVGVEKVTFRKNGEVPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPB8</accession>
    <name>D5GPB8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00011792001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetyltransferase family. ARD1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430373">
        <property type="protein sequence ID" value="CAZ86383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842192.1">
        <property type="nucleotide sequence ID" value="XM_002842146.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPB8"/>
    <dbReference type="FunCoup" id="D5GPB8">
        <property type="interactions" value="740"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GPB8"/>
    <dbReference type="GeneID" id="9186623"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011792001"/>
    <dbReference type="eggNOG" id="KOG3235">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_7_0_1"/>
    <dbReference type="InParanoid" id="D5GPB8"/>
    <dbReference type="OMA" id="RDTLQFK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031415">
        <property type="term" value="C:NatA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990189">
        <property type="term" value="F:protein N-terminal-serine acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990190">
        <property type="term" value="F:protein-N-terminal-glutamate acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000037">
        <property type="entry name" value="N-alpha-acetyltransferase daf-31-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045047">
        <property type="entry name" value="Ard1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23091">
        <property type="entry name" value="N-TERMINAL ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23091:SF4">
        <property type="entry name" value="N-TERMINAL AMINO-ACID N(ALPHA)-ACETYLTRANSFERASE NATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="5">
        <location>
            <begin position="1"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="165"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="165"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ86383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="223" mass="25012" checksum="7280D81E7AB49E23" modified="2010-06-15" version="1">MDIRVLQPSDIPHVQLANITNLPENYFCKYYLYHALSWPQLSYVAVDLSHSSGSPGEPPKIVGYVLAKMEEEPADGVQHGHITSLSVMRTHRRLGLAEKLMRQSQKAMVEAFGAKYVSLHVRVSNKAALHLYRDTLQFKVNKVEGRYYADGENAYSMRKDLTFLEEAESESENEGEEKVEDVGIDGDVPEGGEKKKKKSRAKKYKLGRALGVADLQEVDGRAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPF4</accession>
    <name>D5GPF4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Kynurenine formamidase</fullName>
            <shortName evidence="3">KFA</shortName>
            <shortName evidence="3">KFase</shortName>
            <ecNumber evidence="3">3.5.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Arylformamidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">N-formylkynurenine formamidase</fullName>
            <shortName evidence="3">FKF</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00011808001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the hydrolysis of N-formyl-L-kynurenine to L-kynurenine, the second step in the kynurenine pathway of tryptophan degradation. Kynurenine may be further oxidized to nicotinic acid, NAD(H) and NADP(H). Required for elimination of toxic metabolites.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>N-formyl-L-kynurenine + H2O = L-kynurenine + formate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13009"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:57959"/>
            <dbReference type="ChEBI" id="CHEBI:58629"/>
            <dbReference type="EC" id="3.5.1.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="domain">
        <text evidence="3">The main chain amide nitrogen atoms of the second glycine and its adjacent residue in the HGGXW motif define the oxyanion hole, and stabilize the oxyanion that forms during the nucleophilic attack by the catalytic serine during substrate cleavage.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the kynurenine formamidase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.9" evidence="3"/>
    <dbReference type="EMBL" id="FN430374">
        <property type="protein sequence ID" value="CAZ86397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842206.1">
        <property type="nucleotide sequence ID" value="XM_002842160.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPF4"/>
    <dbReference type="FunCoup" id="D5GPF4">
        <property type="interactions" value="124"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GPF4"/>
    <dbReference type="ESTHER" id="tubmm-d5gpf4">
        <property type="family name" value="Kynurenine-formamidase"/>
    </dbReference>
    <dbReference type="GeneID" id="9186638"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011808001"/>
    <dbReference type="eggNOG" id="ENOG502SAGV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016852_1_0_1"/>
    <dbReference type="InParanoid" id="D5GPF4"/>
    <dbReference type="OMA" id="NARWPDH"/>
    <dbReference type="UniPathway" id="UPA00333">
        <property type="reaction ID" value="UER00454"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004061">
        <property type="term" value="F:arylformamidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034354">
        <property type="term" value="P:'de novo' NAD+ biosynthetic process from L-tryptophan"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019441">
        <property type="term" value="P:L-tryptophan catabolic process to kynurenine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03014">
        <property type="entry name" value="KFase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049492">
        <property type="entry name" value="BD-FAE-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050300">
        <property type="entry name" value="GDXG_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027519">
        <property type="entry name" value="KFase_ver/fungi-typ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN C4A8.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081:SF33">
        <property type="entry name" value="KYNURENINE FORMAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20434">
        <property type="entry name" value="BD-FAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0823">Tryptophan catabolism</keyword>
    <feature type="domain" description="BD-FAE-like" evidence="4">
        <location>
            <begin position="22"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="HGGXW" evidence="3">
        <location>
            <begin position="40"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="123"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="203"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ86397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="263" mass="28757" checksum="8DB891D92C61B11C" modified="2010-06-15" version="1">MTTTLKPPQELPYGSAHPLQSLDLYHQNLDPNTYWVIFIHGGAWRDPKMTKSSGHPLICSLLSKYPHLNAASINYRLSPHPCYPSNGNVARHPNHIDDVRLALSFLQERYSMKREKSVLVGHSAGAYMAFQIDAGCGAAAAAVVGVEGVYHLGKLVEEYADYEEFVGGAFGEGWKGVDEEVLVEVEDGRDGGGRVVLVHSSQDGLLSFGQSKYWAGVLRGRGRVVREVYDVVGNHNEVLEGAELVEVVEEELKGLEVEAGKGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPQ8</accession>
    <name>D5GPQ8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Autophagy protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00011978001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in cytoplasm to vacuole transport (Cvt) and autophagic vesicle formation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Conjugated with ATG12.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Preautophagosomal structure membrane</location>
            <topology evidence="1 6">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ATG5 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430377">
        <property type="protein sequence ID" value="CAZ86501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842310.1">
        <property type="nucleotide sequence ID" value="XM_002842264.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPQ8"/>
    <dbReference type="FunCoup" id="D5GPQ8">
        <property type="interactions" value="321"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GPQ8"/>
    <dbReference type="GeneID" id="9186733"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011978001"/>
    <dbReference type="eggNOG" id="KOG2976">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051894_2_0_1"/>
    <dbReference type="InParanoid" id="D5GPQ8"/>
    <dbReference type="OMA" id="SIQKAVW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034274">
        <property type="term" value="C:Atg12-Atg5-Atg16 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044233">
        <property type="term" value="C:mitochondria-associated endoplasmic reticulum membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061908">
        <property type="term" value="C:phagophore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019776">
        <property type="term" value="F:Atg8-family ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.190">
        <property type="entry name" value="Autophagy protein Apg5, helix rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007239">
        <property type="entry name" value="Atg5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048940">
        <property type="entry name" value="ATG5_HBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042526">
        <property type="entry name" value="Atg5_HR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048939">
        <property type="entry name" value="ATG5_UblA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042527">
        <property type="entry name" value="Atg5_UblA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048318">
        <property type="entry name" value="ATG5_UblB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13040">
        <property type="entry name" value="AUTOPHAGY PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13040:SF2">
        <property type="entry name" value="AUTOPHAGY PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20637">
        <property type="entry name" value="ATG5_HBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20638">
        <property type="entry name" value="ATG5_UblA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04106">
        <property type="entry name" value="ATG5_UblB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0072">Autophagy</keyword>
    <keyword evidence="3 6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <keyword evidence="4 6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Autophagy protein ATG5 UblA" evidence="9">
        <location>
            <begin position="13"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Autophagy protein ATG5 alpha-helical bundle region" evidence="8">
        <location>
            <begin position="138"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="Autophagy protein ATG5 UblB" evidence="7">
        <location>
            <begin position="198"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF20638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ86501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="286" mass="32527" checksum="D922E099410A2DF4" modified="2010-06-15" version="1">MESSDPKSIRRAIWSGSIPCKILLDPGESRVFDASDPYYIIIPRIAYLPFFTERICNFFRPFVIDPEVAKPESAWFEFESVPMKWHWPVGLLYDLFTGRDPAANNVEEDDEHLLPWTLIVHFRDYPAKHLMRLEGPATCYDSWMNTVKEADHVRNGSAKAVMSLSKAESTKLWDSLQSHDFDQFWSVNDKLVSVQARNIPLRLYVPSAPRVVQQPVPPDLPSQEPQTVGTALRTLLPELFPSQRVPVLARPVVHGVVVAMGTPLLELMREAVYPDGFLHISLAMMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>D5G3V6</accession>
    <name>D5G3V6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00003820001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835078.1">
        <property type="nucleotide sequence ID" value="XM_002835032.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5G3V6">
        <property type="interactions" value="781"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G3V6"/>
    <dbReference type="GeneID" id="9187137"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003820001"/>
    <dbReference type="eggNOG" id="KOG0711">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028376_0_1_1"/>
    <dbReference type="InParanoid" id="D5G3V6"/>
    <dbReference type="OMA" id="CSWVVNQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004337">
        <property type="term" value="F:(2E,6E)-farnesyl diphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004161">
        <property type="term" value="F:dimethylallyltranstransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046165">
        <property type="term" value="P:alcohol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045337">
        <property type="term" value="P:farnesyl diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043386">
        <property type="term" value="P:mycotoxin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039702">
        <property type="entry name" value="FPS1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11525:SF0">
        <property type="entry name" value="FARNESYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11525">
        <property type="entry name" value="FARNESYL-PYROPHOSPHATE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ79199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="391" mass="44501" checksum="60A8AF71359EDAD7" modified="2010-06-15" version="1">MATTRPQFEEVFTNVIVPELVAEVRKTQISENAIQWIERNFLHNTLGGKYNRGMTVPDTYRLLAGKETLSLAEYKLSAILGWCTELLQGMFLVADDIMDSSKTRRGSPCWYLMEGVGSIAINDSFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDKIGAKYFKGTDYYIDLVELFHDVTWKTELGQLVDLLTSPEGSIDLNRFNYDKYYFIVRYKTAFYSFYLPVALAMHQAGIATPDNLTRARDVLIPLGEYFQVQDDYLDCYGNPDYIGKIGTDIMDNKCGWLVNKALEIVTPEQRKLLEDNYGQKNAAAEKKVKDLYLELKLEDHYRSYEAESIAMVKGLIDNVDESQGLKRSVLEGFLNKIAGRDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5G400</accession>
    <name>D5G400_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">thioredoxin-dependent peroxiredoxin</fullName>
            <ecNumber evidence="1">1.11.1.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Thioredoxin peroxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00003883001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a hydroperoxide + [thioredoxin]-dithiol = an alcohol + [thioredoxin]-disulfide + H2O</text>
            <dbReference type="Rhea" id="RHEA:62620"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.11.1.24"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the peroxiredoxin family. BCP/PrxQ subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.24" evidence="1"/>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835122.1">
        <property type="nucleotide sequence ID" value="XM_002835076.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G400"/>
    <dbReference type="STRING" id="656061.D5G400"/>
    <dbReference type="GeneID" id="9182370"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003883001"/>
    <dbReference type="eggNOG" id="KOG0855">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042529_2_2_1"/>
    <dbReference type="InParanoid" id="D5G400"/>
    <dbReference type="OMA" id="DMPAHIN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03017">
        <property type="entry name" value="PRX_BCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050924">
        <property type="entry name" value="Peroxiredoxin_BCP/PrxQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42801:SF23">
        <property type="entry name" value="PEROXIREDOXIN DOT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42801">
        <property type="entry name" value="THIOREDOXIN-DEPENDENT PEROXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="6" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="11">
        <location>
            <begin position="130"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="302"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="19"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="302"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ79243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="332" mass="34020" checksum="7E447CB6F8280CFE" modified="2010-06-15" version="1">MSEPVLRRSGRNIAKKEAAPPPPAPGPAPKKRASTEPKKPSAKKARTVVDKAPTVVKNAVSKDKDSKKEDASKKKPEGEASASAAAAEVKAAPIPAAESSSAAPTVTTTIVTTTAGTKSAAKAKSGKAKAKRADAAEEAAAALGDGDLLPNTLPEVQTHDGGKTTLTAILAQASKGIVIFAYPKASTPECTTQACLFRDNYTAFTDAGYSVYGLSGDQPVANEKFRNKQNLTYTLLCDPTYELHEKLGIKKSPKGTVRSVIIIEKTSGDAAAATHPGKILRKTKASAHVSLQVAKEVVGLDGTKKDAEGGGKKDAAGDVTLPDAGAAGEGVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>D5G473</accession>
    <name>D5G473_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00003977001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835195.1">
        <property type="nucleotide sequence ID" value="XM_002835149.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G473"/>
    <dbReference type="GeneID" id="9184292"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003977001"/>
    <dbReference type="eggNOG" id="ENOG502SIF7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070647_1_1_1"/>
    <dbReference type="InParanoid" id="D5G473"/>
    <dbReference type="OMA" id="YPCGGIP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21176">
        <property type="entry name" value="LPMO_auxiliary-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046936">
        <property type="entry name" value="BIM1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046530">
        <property type="entry name" value="BIM1-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34992:SF10">
        <property type="entry name" value="COPPER ACQUISITION FACTOR BIM1-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34992">
        <property type="entry name" value="HYPHAL ANASTAMOSIS-7 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20238">
        <property type="entry name" value="BIM1-like_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003071984" evidence="8">
        <location>
            <begin position="27"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Copper acquisition factor BIM1-like" evidence="9">
        <location>
            <begin position="26"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF20238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ79316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="219" mass="23944" checksum="44648E72F8DDE5F6" modified="2010-06-15" version="1">MSRSSRPFSWKLVLVALLATVGLATAHFELQYPPWRGNTLKDDMQWRYPCGGIPVTTNRTKWPLKGGDISIVPGWNAGHPSAFFYVNMGFGSTPPNMTNVMQPIFQMTGPSRDPYPGSFCLRDVPLPVNASVKAGDNATIQVIQVALHGASLYNCADITFVEPGEEDKMPDGMCVNSTTFNFNLVYTTRSSSSISSKNLLDKTTMITVLLGGLFLGAWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4C6</accession>
    <name>D5G4C6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00004049001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4C6"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004049001"/>
    <dbReference type="HOGENOM" id="CLU_3433198_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4C6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ79369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="16" mass="1829" checksum="BBCEB543EFD8FC0F" modified="2010-06-15" version="1">MRMELSQSDVMMVQSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4K8</accession>
    <name>D5G4K8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00004202001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429988">
        <property type="protein sequence ID" value="CAZ79451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835294.1">
        <property type="nucleotide sequence ID" value="XM_002835248.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4K8"/>
    <dbReference type="GeneID" id="9188393"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004202001"/>
    <dbReference type="HOGENOM" id="CLU_1603951_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4K8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ79451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="166" mass="18862" checksum="9652003EAB44F366" modified="2010-06-15" version="1">MVAYVGINDGGRGWEKGTDEPEELLVLVFVWHRVARLIRPCPAACEVDRTGLRLFTVGEGGRYDTRVPYRNSIIRIPYLCWQQVLYSSIIQYLYSTSKAHGAMGIGPRDGRQSPPLHTSSSLLRYRSFIKFQNATDGTTVPYRYAIYLIPIPSHNCTLTLYYVTLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4Q4</accession>
    <name>D5G4Q4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Small nuclear ribonucleoprotein E</fullName>
            <shortName evidence="11">snRNP-E</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Sm protein E</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00000050001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Involved in pre-mRNA splicing. Binds and is required for the stability of snRNA U1, U2, U4 and U5 which contain a highly conserved structural motif called the Sm binding site. Involved in cap modification.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429990">
        <property type="protein sequence ID" value="CAZ79490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835333.1">
        <property type="nucleotide sequence ID" value="XM_002835287.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4Q4"/>
    <dbReference type="FunCoup" id="D5G4Q4">
        <property type="interactions" value="863"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G4Q4"/>
    <dbReference type="GeneID" id="9188426"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000050001"/>
    <dbReference type="eggNOG" id="KOG1774">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125186_1_1_1"/>
    <dbReference type="InParanoid" id="D5G4Q4"/>
    <dbReference type="OMA" id="VPPINCI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005687">
        <property type="term" value="C:U4 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990935">
        <property type="term" value="F:splicing factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036261">
        <property type="term" value="P:7-methylguanosine cap hypermethylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01718">
        <property type="entry name" value="Sm_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000031">
        <property type="entry name" value="Small nuclear ribonucleoprotein E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027078">
        <property type="entry name" value="snRNP-E"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11193">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 11" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8 11" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 11" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="12">
        <location>
            <begin position="17"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU365053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ79490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="93" mass="10523" checksum="88B69CF74A66BEB7" modified="2010-06-15" version="1">MASRGGGGGRRVLLPPINFIFKLLQSHATVQIWLYEQLAIRIEGKIRGFDEFMNLVIDDAVEVKLKTKDEEESRRELGQILLKGDNVSLIQSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4Q8</accession>
    <name>D5G4Q8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00000056001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429990">
        <property type="protein sequence ID" value="CAZ79494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835337.1">
        <property type="nucleotide sequence ID" value="XM_002835291.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4Q8"/>
    <dbReference type="STRING" id="656061.D5G4Q8"/>
    <dbReference type="GeneID" id="9188430"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000056001"/>
    <dbReference type="eggNOG" id="ENOG502QW4G">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035426_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4Q8"/>
    <dbReference type="OMA" id="YYDNQQW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="214"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="273"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="298"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="341"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="358"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="380"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="402"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="435" mass="47071" checksum="79BC10139C91B112" modified="2010-06-15" version="1">MSYYESQTSWQNQSNRPNWDPRAAGSTPVQAHFPRSHASDPSDLTVRYTASMKPEDPSAFGSQLDEVDRAVDNLVKSGKMFAPQRRDSLPVMGPRGFPVEHDARMVAPMPSRHYSVQEFDVMRSQSAANLQNFYATQRFQPRPTEAEQMMQAKRRLAAQRERDLRNYHQEQQYNRTLLAEVSGYGKDRVMSPGAMSEDERRELIARQHRALYTSGHSEGSHSEDGVPLDEGNPPRGTNPNSNGPSAPGSNAGGTRGPSPMSSAFDPFGHQNGRIDSNAVQTSGDQSQTGNAISPPIRSRTNSASSPSSKSASFSLFDNAQAQSSRTSASSPGGSPPRSQRISTTPVTSSGVAPIGTRPNAQNQSGVANNSKRASPPLNSPRYNLQQENDPFNNSNVNKERSSPAPSNNSAPNGQGDGLGWSKVWGSGTKGLSVWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4R9</accession>
    <name>D5G4R9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00000069001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429990">
        <property type="protein sequence ID" value="CAZ79505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835348.1">
        <property type="nucleotide sequence ID" value="XM_002835302.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4R9"/>
    <dbReference type="GeneID" id="9188441"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000069001"/>
    <dbReference type="HOGENOM" id="CLU_2098611_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4R9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ79505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="116" mass="12654" checksum="6F8F8AB7D597C9F4" modified="2010-06-15" version="1">MKGGFASQILTILAEPGTSSQLPLWVGTIKYSNIFSQSLNQIYQKKNPDCSGDPKLCFLHRTGTCTRVKYDSLCPHHPPPLPPPVRCLSPPSYPPAPALWKATQTHPARNNRCGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4T8</accession>
    <name>D5G4T8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UTP--glucose-1-phosphate uridylyltransferase</fullName>
            <ecNumber evidence="3">2.7.7.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">UDP-glucose pyrophosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00000195001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a central role as a glucosyl donor in cellular metabolic pathways.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>alpha-D-glucose 1-phosphate + UTP + H(+) = UDP-alpha-D-glucose + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19889"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.7.7.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UDPGP type 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.9" evidence="3"/>
    <dbReference type="EMBL" id="FN429991">
        <property type="protein sequence ID" value="CAZ79531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835374.1">
        <property type="nucleotide sequence ID" value="XM_002835328.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4T8"/>
    <dbReference type="FunCoup" id="D5G4T8">
        <property type="interactions" value="825"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G4T8"/>
    <dbReference type="GeneID" id="9183013"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000195001"/>
    <dbReference type="eggNOG" id="KOG2638">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023632_3_0_1"/>
    <dbReference type="InParanoid" id="D5G4T8"/>
    <dbReference type="OMA" id="KEYCFLS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003983">
        <property type="term" value="F:UTP:glucose-1-phosphate uridylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006078">
        <property type="term" value="P:(1-&gt;6)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006011">
        <property type="term" value="P:UDP-alpha-D-glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00897">
        <property type="entry name" value="UGPase_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.10.10:FF:000001">
        <property type="entry name" value="UTP--glucose-1-phosphate uridylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000002">
        <property type="entry name" value="UTP--glucose-1-phosphate uridylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002618">
        <property type="entry name" value="UDPGP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016267">
        <property type="entry name" value="UDPGP_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43511">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01704">
        <property type="entry name" value="UDPGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="138"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="154"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ79531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="665" mass="74131" checksum="C3621FA3E3B7E31A" modified="2010-06-15" version="1">MASFTSRWCPVLCFRHPRQACNIPLTLRPPRRSASRSFRPSLSLLHSFLFSPFLTPISQQANSSSSPALLNNSLCKKAIESTTFHPSSIPLPFLQQEVQERTFQALQQEPVDVKPVEAKPIQTVQTVQTVQTATAIPTEQQQPEKMSSFKKSLPVEGGAPGEGLAPPGRRHGKSQSAFAFDNTSTSVAASQMRNALNGLADSVKDPKTKVAFETEMDNFFALFRRYLQDKAKGTTLDWDRIQPPQSSQVIPYDTLPRVEDPKLLSKLAVLKLNGGLGTSMGCVGPKSVIEVREGMSFLDLSVRQVEYLNRTYNVDVPFVLMNSFNTDDDTQNIIKKYEGHRVTIHTFNQSRYPRVLKDSLLPATKDYHSPIVDWYPPGHGDVFESLQNSGILDSLLEKDIEYLFLSNVDNLGAVVDLSILDHMRETGAEYIMELTDKTKADVKGGTIIDYEGTVRLLEIAQVPKEHEQDFKSIKKFKYFNTNNIWLNLKAIKRVVENHELELEIIPNSKTIPVDKKGESDISVIQLETAVGAAIRHFKNAHGVNVPRRRFLPVKTCSDLMLVKSDLYSMKHGQLMMDVARFGPAPLIKLGSHFKKVSDFQKRVPSIPRILELDHLTVTGAVNLGRGVTLKGTVIIVANEGSTIDIPPGSVLENVVVQGSLRLLEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4Y6</accession>
    <name>D5G4Y6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00000156001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429993">
        <property type="protein sequence ID" value="CAZ79579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835422.1">
        <property type="nucleotide sequence ID" value="XM_002835376.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4Y6"/>
    <dbReference type="STRING" id="656061.D5G4Y6"/>
    <dbReference type="GeneID" id="9183061"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000156001"/>
    <dbReference type="eggNOG" id="ENOG502RSJA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019101_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4Y6"/>
    <dbReference type="OMA" id="FFFARIT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF2">
        <property type="entry name" value="SRPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="115"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="150"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="192"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="227"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="8">
        <location>
            <begin position="41"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="301"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="335"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="306"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="335"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ79579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="397" mass="43167" checksum="B27BA2F012353CA0" modified="2010-06-15" version="1">MAPTIAFAALRARDSGRRGGPDPVLVEGWTLYALGVSVILARLVTRYKMVGVEGSKPDDWLMILVMIIYGIESTMSHLVSLYGGNSNLSPAQRAGMSEEEVQRRVFGSKMFMVGWFSYSGAMWVLKLCMVFFFARITVGLRRQVWITTAYVVVGMSYFVICLVLFLTCRPWHKLWQIYPDPGSNCSIESPVYYLTVLSFNVLTDAFLISLPLPLLIRSSLPWRRKAILVLLFSAGIFVITAAVLRCVFSLTDPQNSRTVSAWACRETFVAVVVGNAPMLKQLLTKRAWSKARIATARCVGAGTGSSGSPLSRQVGGSKSGDATLVDKSAVEPGLEIHRKTEMSVESADRATLEDDGPPGVNGLGELSGCFYKAICESSAASSCASSRHTASGERGDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5G516</accession>
    <name>D5G516_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00000322001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429995">
        <property type="protein sequence ID" value="CAZ79609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835452.1">
        <property type="nucleotide sequence ID" value="XM_002835406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G516"/>
    <dbReference type="STRING" id="656061.D5G516"/>
    <dbReference type="GeneID" id="9186307"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000322001"/>
    <dbReference type="eggNOG" id="KOG2408">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002329_1_0_1"/>
    <dbReference type="InParanoid" id="D5G516"/>
    <dbReference type="OMA" id="NTEYART"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20612">
        <property type="entry name" value="CYP_LDS-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09817">
        <property type="entry name" value="linoleate_diol_synthase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.640.10">
        <property type="entry name" value="Haem peroxidase domain superfamily, animal type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019791">
        <property type="entry name" value="Haem_peroxidase_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037120">
        <property type="entry name" value="Haem_peroxidase_sf_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050783">
        <property type="entry name" value="Oxylipin_biosynth_metab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034812">
        <property type="entry name" value="Ppo-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11903:SF13">
        <property type="entry name" value="LINOLEATE 10R-LIPOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11903">
        <property type="entry name" value="PROSTAGLANDIN G/H SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03098">
        <property type="entry name" value="An_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00457">
        <property type="entry name" value="ANPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50292">
        <property type="entry name" value="PEROXIDASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="5" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="50"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="59"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="421"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR619791-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ79609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1119" mass="126528" checksum="052125122A9DC643" modified="2010-06-15" version="1">MASNGDGTNGATNGDSKYATQSKPPPPVAKPTRAAVDSTFEQFSNLLHASNRPLPNRFGDGKDQRPPELKPTGVLTDINTLRRGGFFWESVGTLWDFLKQKAKGGAVDDKTMIMERVIQLTSRLPPTSKARVALTTLQVDQLWDSLQHPPLSYLGDEFSYRQADGGYNNIMYPQLGRAGSAYARSVKPMTKMPGAPPDAYTLFDRIFSRGPNDEHFREHDNNVSSMLFYTATIIIHDLFRTNRENQNISDTSSYLDLAPLYGSSVEDQSKIRTFQDGKIKPDSYSEKRLLAFPPGVSVLLIMFGRFHNYVVENLKAINEGGRFNLKFPRYPNGDDEVTRQAKALRQQDEDLFQTGRLVTCGLYINFILNDYLRTIVNLNRVDTTWTLDPRFEASKVYNPDGTPAGIGNMVSAEFNLIYRWHSCISKRDDQWTKDFYQGLFPGRDASDIEMPEFTRGVGRWERSLSDDPIQRNIAGLERKADGSYHDDDLVKILTESVEDVAGAFGARNVPHVLRLVEVLGIEQSRKWMVGSLNELREFFGLKPHATFEDINPDPAVANTLRQLYDHPDFVEMYAGLVAEADKKPMVPGVGIGPTYTISRAILSDAVTLVRGDRFYTADYTASHLTNWGLQEASSDLATVYGCVGYKLILKAFPNHFKFNSIYAHYPLTIPQENHKIHTALNSVDQFDFERPVYTPPRIPISSYNATKQILCDAENFKVTWGAGFDFIMRADFMLSGDKPSNSEQKQFVRDRLYLGGVDWKQQIRQFYEEATEKLIRKKAYSLGDTYQVDAVRDIGNIAQTIFAASIFNLPMKSEDHPKGIYTEQELYMILCVMFIVIFFDIDSSKSFPLRQAGFKVVRQYGTLVEAQVKAIKNWSWLQGVWDPLNIRGRNKSSPLKSYGWTMIKRLLDTGKSPYDVTWSYIVPTAGASAPNQGQIFAQVLDFYLEDRNSHHLAEIQRLAQSGAVDAWETIKKYALEGGRLAGTFGLYRRVEPDNITIEDNGRNIELRKGDMVFVNFITASRDPVVFPDPLEIKLDRPEASYMQYGDGPHECLGKAANIIGLTTMLMQFGKLKGLRRAPGPQGALKYIPKPGGFKVYMKEDWSAYWPFPTSMKVRFDDII</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5V6</accession>
    <name>D5G5V6_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Fructose-bisphosphate aldolase</fullName>
            <shortName evidence="9">FBP aldolase</shortName>
            <ecNumber evidence="4 9">4.1.2.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00001593001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>beta-D-fructose 1,6-bisphosphate = D-glyceraldehyde 3-phosphate + dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:14729"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.1.2.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9">Binds 2 Zn(2+) ions per subunit. One is catalytic and the other provides a structural contribution.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the class II fructose-bisphosphate aldolase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.13" evidence="4 9"/>
    <dbReference type="EMBL" id="FN430002">
        <property type="protein sequence ID" value="CAZ79899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835742.1">
        <property type="nucleotide sequence ID" value="XM_002835696.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5V6"/>
    <dbReference type="STRING" id="656061.D5G5V6"/>
    <dbReference type="GeneID" id="9187577"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001593001"/>
    <dbReference type="eggNOG" id="KOG4153">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1403360_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5V6"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00183"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004332">
        <property type="term" value="F:fructose-bisphosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000771">
        <property type="entry name" value="FBA_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006411">
        <property type="entry name" value="Fruct_bisP_bact"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30559:SF0">
        <property type="entry name" value="FRUCTOSE-BISPHOSPHATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30559">
        <property type="entry name" value="FRUCTOSE-BISPHOSPHATE ALDOLASE CLASS 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01116">
        <property type="entry name" value="F_bP_aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00602">
        <property type="entry name" value="ALDOLASE_CLASS_II_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="8 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU366023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ79899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="194" mass="21224" checksum="B051BB6D8486357B" modified="2010-06-15" version="1">MSIQLYIPPPLQLRQSQQIRHLRRCQSSPPTPSTVAAALEAARDAHSPVTLQTSQGGAAYFASKGVPNSNQTASISGAIAAVRYIRAIAPAYQIPVILHTDHCAAKLLPWLDGMVSADEAYFAEQGEPLFSSHMIDLSEEDKDFNIATTKEYLPRPAPMKQWLEMEIRITGCEEDGVDNTGVDNSALYTQPEDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5W2</accession>
    <name>D5G5W2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00001599001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SPT2 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430002">
        <property type="protein sequence ID" value="CAZ79905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835748.1">
        <property type="nucleotide sequence ID" value="XM_002835702.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5W2"/>
    <dbReference type="STRING" id="656061.D5G5W2"/>
    <dbReference type="GeneID" id="9187583"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001599001"/>
    <dbReference type="eggNOG" id="ENOG502RI81">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_619926_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5W2"/>
    <dbReference type="OMA" id="QHKKVEK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013256">
        <property type="entry name" value="Chromatin_SPT2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08243">
        <property type="entry name" value="SPT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00784">
        <property type="entry name" value="SPT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="18"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="18"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="262"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="282"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="295"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="347"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ79905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="442" mass="46758" checksum="EB251DFB89E76E6E" modified="2010-06-15" version="1">MVFNDSNPSFSISSILSRYDSSATATTPSTISKPLPTKKLVNNARPNGTGSGPITSTTTSPPRSSLSSTSKSAASQMSRDTTTKVVTSSKPDINSIKRRKTEEVPKSSLELLKERKELLQRKSGGVTTSTSTAVSNSTSKVAPPRLSTGAATARPGSGIRTAGTAKPVAEAPKPMSYRERMAAALQAGAEKKPAAGTITHKARPLVKEKKAWQLKLEGQGEGKGADTMAGRNSKSPGVSGGEKAASTSKKTADIAKPVTKKSSNGANGSANGVGSANGANGRKSSIGNGVMSSLKGKDKDRGKHLEAGMKRKRESPPPKSGYSSYSRNKYSTNHKRYGYGPPRPDSYEEDDPDDDFVVNDEDDEDDAYGRGGGYGGFAKKKYSYIDYDSASDMEATGADVLEEEERSRRVAAKEDAAQEKFERELAAKKAALKKKGVLTKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>D5G629</accession>
    <name>D5G629_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00001745001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430007">
        <property type="protein sequence ID" value="CAZ79972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835815.1">
        <property type="nucleotide sequence ID" value="XM_002835769.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G629"/>
    <dbReference type="STRING" id="656061.D5G629"/>
    <dbReference type="GeneID" id="9187651"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001745001"/>
    <dbReference type="HOGENOM" id="CLU_750460_0_0_1"/>
    <dbReference type="InParanoid" id="D5G629"/>
    <dbReference type="OMA" id="DWRHERK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="184"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="24"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="196"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="272"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="332"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="343"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="369" mass="39989" checksum="A9A2117B18A09746" modified="2010-06-15" version="1">MANLQAWHYGAREDGHYARHSDEPNSPVSPISPSPYNTQLTPPGSGYVDASGYGYNQSPPTHPPPSYPTQASEYQSSDTYLSPGARGYLQTFGGTSAAVLTHSRPSSEHGKYPYETSTATAGEYPNYDSRDPPLNYDPANPPPGGDRGLGSMMVGAAGERFMHHSGGSFLGAATGALAAGVVGYEVGKHRKKKKERSKEKKERAKEKKEKKEKKKRAKNDEYEKREKHERREKKERREKIEGHSGHDSSFKKEGRHRHDSSSSSSGGGAYHTHSHASSHASSHSHKHSSSHRTHGGSADEKKSGALSGLYAYGYSTGGGHVFSRRKGERDRFRKSRHHSRHGSVSSYSSSSSSSSSSSSSSSSSSCDSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6C2</accession>
    <name>D5G6C2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 15">Non-structural maintenance of chromosomes element 1 homolog</fullName>
            <ecNumber evidence="4 15">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">GSTUM_00004419001</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Acts in a DNA repair pathway for removal of UV-induced DNA damage that is distinct from classical nucleotide excision repair and in repair of ionizing radiation damage. Functions in homologous recombination repair of DNA double strand breaks and in recovery of stalled replication forks.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Component of the Smc5-Smc6 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the NSE1 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4 15"/>
    <dbReference type="EMBL" id="FN430009">
        <property type="protein sequence ID" value="CAZ80065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835908.1">
        <property type="nucleotide sequence ID" value="XM_002835862.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6C2"/>
    <dbReference type="STRING" id="656061.D5G6C2"/>
    <dbReference type="GeneID" id="9187436"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004419001"/>
    <dbReference type="eggNOG" id="KOG4718">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045153_0_1_1"/>
    <dbReference type="InParanoid" id="D5G6C2"/>
    <dbReference type="OMA" id="ACINHSC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030915">
        <property type="term" value="C:Smc5-Smc6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16493">
        <property type="entry name" value="RING-CH-C4HC3_NSE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011513">
        <property type="entry name" value="Nse1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014857">
        <property type="entry name" value="Nse1_RING_C4HC3-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20973">
        <property type="entry name" value="NON-SMC ELEMENT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20973:SF0">
        <property type="entry name" value="NON-STRUCTURAL MAINTENANCE OF CHROMOSOMES ELEMENT 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07574">
        <property type="entry name" value="SMC_Nse1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08746">
        <property type="entry name" value="zf-RING-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12 15" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="13 15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 15" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11 15" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Non-structural maintenance of chromosomes element 1 RING C4HC3-type" evidence="17">
        <location>
            <begin position="218"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="260"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="284"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="318"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="330"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU368018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF08746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAZ80065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="343" mass="38266" checksum="0D3C1DFE27DCB027" modified="2010-06-15" version="1">MSRRVMKVDDIKILLAEILSADDAEHEIGTEDINDRIVSSYISKANDMIHDFDLEIKIMLDQRDRSKTFALINTTSDELVQLATTHNADEIAFFKRVLDAIFETNNTRDAEVLAVHDFAAVRLHKNPQQGGTSQATQGPASQSVSGLTMVGAEAALRSFVDEGWLECSKAGFYSLTTRAVLELGPYLFQTYNTPADEDDEQEDDGSSTNGIIERIKTCHACREIVTIGLRCIDKHCKLRLHTPCAEGIFRPNRTHECPRCKKEWSGGLPVGEEADRGQRRPRGNRQSNVGTTPRSRVNSVPHPTSTRPVMRGARDTPIEEDSQEEEQDALGERNDDHDVEMTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6P7</accession>
    <name>D5G6P7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00002157001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430012">
        <property type="protein sequence ID" value="CAZ80190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836033.1">
        <property type="nucleotide sequence ID" value="XM_002835987.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6P7"/>
    <dbReference type="GeneID" id="9187216"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002157001"/>
    <dbReference type="eggNOG" id="ENOG502S6ZS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_839295_0_0_1"/>
    <dbReference type="InParanoid" id="D5G6P7"/>
    <dbReference type="OMA" id="IVPTIHE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018828">
        <property type="entry name" value="RRG7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28133">
        <property type="entry name" value="REQUIRED FOR RESPIRATORY GROWTH PROTEIN 7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28133:SF1">
        <property type="entry name" value="REQUIRED FOR RESPIRATORY GROWTH PROTEIN 7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10356">
        <property type="entry name" value="RRG7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="224"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="226"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ80190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="267" mass="29570" checksum="50D28DB87E2182CD" modified="2010-06-15" version="1">MRATLQRYFISHPILLANTNKRYSRKAVGAIRTSDLHYDLESFLKYAKGIALPTHTTVYVGTLYEYTVKEALKSFGMSLERTGGRDDRGVDLRGTWTIPNLPSEQSQKPRVYDALVQCKCTKIHPAAIRELDGTVSSKKPETIGILAAPEHCSPGIRKHMSMSPRPLMYLCLGGKEGIVEQIIWNTSASVLLPGVGVRAIHLKDRTEIVPTIHEHEFRILLSQTSPEKETVEGVENGREKEETPVLQSGSGDTAPGTTIVDYGRSNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6R8</accession>
    <name>D5G6R8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Citrate synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00002237001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>oxaloacetate + acetyl-CoA + H2O = citrate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16845"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16947"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>propanoyl-CoA + oxaloacetate + H2O = (2S,3S)-2-methylcitrate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23780"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57392"/>
            <dbReference type="ChEBI" id="CHEBI:58853"/>
            <dbReference type="EC" id="2.3.3.5"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the citrate synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430014">
        <property type="protein sequence ID" value="CAZ80211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836054.1">
        <property type="nucleotide sequence ID" value="XM_002836008.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6R8"/>
    <dbReference type="FunCoup" id="D5G6R8">
        <property type="interactions" value="904"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G6R8"/>
    <dbReference type="GeneID" id="9187236"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002237001"/>
    <dbReference type="eggNOG" id="KOG2617">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022049_2_1_1"/>
    <dbReference type="InParanoid" id="D5G6R8"/>
    <dbReference type="OMA" id="VLEWLFK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050440">
        <property type="term" value="F:2-methylcitrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036440">
        <property type="term" value="F:citrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006101">
        <property type="term" value="P:citrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.230.10:FF:000001">
        <property type="entry name" value="Citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.580.10:FF:000001">
        <property type="entry name" value="Citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.580.10">
        <property type="entry name" value="Citrate Synthase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.230.10">
        <property type="entry name" value="Cytochrome P450-Terp, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016142">
        <property type="entry name" value="Citrate_synth-like_lrg_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016143">
        <property type="entry name" value="Citrate_synth-like_sm_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002020">
        <property type="entry name" value="Citrate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019810">
        <property type="entry name" value="Citrate_synthase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010109">
        <property type="entry name" value="Citrate_synthase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036969">
        <property type="entry name" value="Citrate_synthase_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01793">
        <property type="entry name" value="cit_synth_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007128">
        <property type="entry name" value="PRK09569.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11739">
        <property type="entry name" value="CITRATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11739:SF8">
        <property type="entry name" value="CITRATE SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00285">
        <property type="entry name" value="Citrate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00143">
        <property type="entry name" value="CITRTSNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48256">
        <property type="entry name" value="Citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00480">
        <property type="entry name" value="CITRATE_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="active site" evidence="6">
        <location>
            <position position="308"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="354"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00049052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR610109-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ80211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="472" mass="52202" checksum="406270EBAAD89C2E" modified="2010-06-15" version="1">MAAPMRIGALALRNSLSASASFSIKRAGFRYYSTVKPKTLKETFAAKIPDEIEKIKKLKKEHGSKVLGEVTLDQVYGGARGIKALVWEGSVLDAEEGIRFRGKTIPECQAILPKAPGGEEPLPESIFWLLLTGEVPTEQQVRELSAEWAARSSIPKFVEELIDRCPNTLHPMAQFSLAVTALEHESQFAKAYAKGLNKKEYWNYTFEDSMDLIAKLPTIAAKIYRNIFKDGKVVPIQMDKDYGFNLANQLGFGDNKDFVDLMRLYLVIHSDHEGGNVSAHTTHLVGSALSSPYLSLAAGLNGLAGPLHGLANQEVLNWLTEMKKKVGDDLSDESIKNYLWDTLNAGRVVPGYGHAVLRKTDPRYMSQREFALKKLPEDPMFKLVSQVYNIAPGVLTEHGKTKNPYPNVDAHSGVLLQYYGLTEASYYTVLFGVSRALGVLPQLIIDRAVGAPIERPKSFSTEKYAQLVGAKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5G745</accession>
    <name>D5G745_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">acetate--CoA ligase</fullName>
            <ecNumber evidence="3">6.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00002338001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetate + ATP + CoA = acetyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23176"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.1" evidence="3"/>
    <dbReference type="EMBL" id="FN430021">
        <property type="protein sequence ID" value="CAZ80338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836147.1">
        <property type="nucleotide sequence ID" value="XM_002836101.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G745"/>
    <dbReference type="FunCoup" id="D5G745">
        <property type="interactions" value="688"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G745"/>
    <dbReference type="GeneID" id="9184272"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002338001"/>
    <dbReference type="eggNOG" id="KOG1175">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_3_6_1"/>
    <dbReference type="InParanoid" id="D5G745"/>
    <dbReference type="OMA" id="TVHTKKI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003987">
        <property type="term" value="F:acetate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006085">
        <property type="term" value="P:acetyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05966">
        <property type="entry name" value="ACS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000004">
        <property type="entry name" value="Acetyl-coenzyme A synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032387">
        <property type="entry name" value="ACAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095:SF14">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16177">
        <property type="entry name" value="ACAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acetyl-coenzyme A synthetase N-terminal" evidence="9">
        <location>
            <begin position="39"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="7">
        <location>
            <begin position="97"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="7">
        <location>
            <begin position="291"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="8">
        <location>
            <begin position="486"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ80338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="606" mass="67972" checksum="EB717C1C5C103982" modified="2010-06-15" version="1">MSEAEVSVVSEAHNVETYQVPRKYYEKHPAKPHLTLEEYKAQYRESIENPDKFWGEKARELLHWDIPFQTVRNGSFQHGDISWFLEGRLNACYNCVDRHAFKDPERPAIIYEADEPSEGRVITYSELLREVCKLAFVLRQMGVRKGDTVAIYMPMIPEALVAFLAIVRIGAVHSVVFAGFSAESLRDRVLDARSKVVITTDEGKRGGKVIGTKRIVDDALKQCPEVKNVLVYRRTGSEVPWTQGRDYWWHEEVNKYPGYCPPTLMDSEDPLFLLYTSGSTGKPKGGTPAYPDFSRYWDIIEKHGITQFYVAPTALRLLKKAGDHHINKQMKTLRVLGSVGEPIAEDVWKWYHEKVGRGECHVVDTYWQTETGSHAIAPMAGVTPTKPGSATLPFFGIEPAIIDPVTGDELHGNGVEGVLAIKRPWPSMARTVWASHQRFMETYLNVYKGYYFTGDGAGRDHEGYYWIRGRVDDVVNVSGHRLSTAEIEAALIHHEAVAEAAVVGIADELTGQTVNAFVSLKDSFENSSEMRKSLTLQVRKSIGPFAAPKAVHVVNDLPKTRSGKIMRRVLRKILSGEEDQLGDTSTLSDPSVVGTILETLHETGNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7D0</accession>
    <name>D5G7D0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">1,3-beta-glucanosyltransferase</fullName>
            <ecNumber evidence="9">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00002553001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Splits internally a 1,3-beta-glucan molecule and transfers the newly generated reducing end (the donor) to the non-reducing end of another 1,3-beta-glucan molecule (the acceptor) forming a 1,3-beta linkage, resulting in the elongation of 1,3-beta-glucan chains in the cell wall.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cell membrane</location>
            <topology evidence="1 9">Lipid-anchor</topology>
            <topology evidence="1 9">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glycosyl hydrolase 72 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="9"/>
    <dbReference type="EMBL" id="FN430024">
        <property type="protein sequence ID" value="CAZ80423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836232.1">
        <property type="nucleotide sequence ID" value="XM_002836186.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7D0"/>
    <dbReference type="FunCoup" id="D5G7D0">
        <property type="interactions" value="20"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G7D0"/>
    <dbReference type="GeneID" id="9185321"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002553001"/>
    <dbReference type="eggNOG" id="ENOG502QRZZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021855_1_0_1"/>
    <dbReference type="InParanoid" id="D5G7D0"/>
    <dbReference type="OMA" id="GVNDYSW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042124">
        <property type="term" value="F:1,3-beta-glucanosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071970">
        <property type="term" value="P:fungal-type cell wall (1-&gt;3)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000032">
        <property type="entry name" value="1,3-beta-glucanosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004886">
        <property type="entry name" value="Glucanosyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468:SF5">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03198">
        <property type="entry name" value="Glyco_hydro_72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 9" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8 9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6 9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005126596" description="1,3-beta-glucanosyltransferase" evidence="9">
        <location>
            <begin position="23"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="420"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="319"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="364"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="377"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="394"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ80423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="446" mass="48084" checksum="0FCBD7117518A4CF" modified="2010-06-15" version="1">MKGLTATLVATSSLLFSGIASSQITEPVTVKGNAFFVGNDRFYIRGVDYQPGGSSDLADPLSNTTVCKRDISYMKDLGLNTIRVYTSDNSASHDECMELLSEAGIYLILDVNAPKFSISRDFPVASYNEVYLQHVFATIDVFSKYTNTLGFFAANEVINAANSTRAAAVVKALIRDMRKYIAKHSDRPIPVGYSAADVAENRREMAHYLNCGSDDVRADFFAFNDYSWCGPSSFVQSGWNKKVENYTDYSIPLFLSEFGCIEVKPRTFSEVKTLYSTQMTGVFSGGLVYEFTQEPNNYGLVQIDGDNVTALTDYDNLKKQYSSTKNPPGDGGYQKDLPPSDCPERTFLWEASNTLPDFPSAASAYMDNGAGKPKGTNGVSSQFVPQSQKDYNLPGESGAPAPSSSGSANQSDQGAAAGGMAGLAAGGLWMGIVVGLGTIAGVMLLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7Q5</accession>
    <name>D5G7Q5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">GSTUM_00004679001</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the DEAD box helicase family. DECD subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="FN430031">
        <property type="protein sequence ID" value="CAZ80548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836357.1">
        <property type="nucleotide sequence ID" value="XM_002836311.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7Q5"/>
    <dbReference type="FunCoup" id="D5G7Q5">
        <property type="interactions" value="1191"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G7Q5"/>
    <dbReference type="GeneID" id="9187027"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004679001"/>
    <dbReference type="eggNOG" id="KOG0329">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_1_0_1"/>
    <dbReference type="InParanoid" id="D5G7Q5"/>
    <dbReference type="OMA" id="YAHVEPK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17950">
        <property type="entry name" value="DEADc_DDX39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000809">
        <property type="entry name" value="ATP-dependent RNA helicase SUB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000111">
        <property type="entry name" value="DEAD-box ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="18">
        <location>
            <begin position="59"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="16">
        <location>
            <begin position="90"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="17">
        <location>
            <begin position="277"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="14">
        <location>
            <begin position="59"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAZ80548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="443" mass="49701" checksum="338C70D40BE0C400" modified="2010-06-15" version="1">MSGEEDLIDYSDDEGLDATKETPSVAANGEAAAKKADVTAAAGGPAADKKGSYVGIHSTGFRDFLLKPELLRAIVDCGFEHPSEVQQVCIPQSILGTDVLCQAKSGLGKTAVFVLTTLQQVDPVPGEASVLVMCHTRELAYQIKNEYARFSKYMPEVRTAVFYGGTPMQNDVQVLKNKDQHPHIIVATPGRLNALVRDKHLRLGSVKVFVLDECDKMLEQIDMRRDVQEIFRATPQQKQVMMFSATLSQEIRPICKKFMQSPLEIYVDDETKLTLHGLQQYYVKLEEKEKNRKLNELLDQLEFNQVIIFVKSTIRATELSRLLNECNFPSIAVHSGIAQEERIARYKQFKEFNKRICVATDVFGRGIDIERINLAINYDLPADPDSYLHRVGRAGRFGTKGLSISFVSSKEDAEVLDKIMERFEVSLDEFPESGIDSSSYMNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7R8</accession>
    <name>D5G7R8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00004695001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430031">
        <property type="protein sequence ID" value="CAZ80561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836370.1">
        <property type="nucleotide sequence ID" value="XM_002836324.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7R8"/>
    <dbReference type="STRING" id="656061.D5G7R8"/>
    <dbReference type="GeneID" id="9181380"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004695001"/>
    <dbReference type="eggNOG" id="ENOG502SDHI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_619927_0_0_1"/>
    <dbReference type="InParanoid" id="D5G7R8"/>
    <dbReference type="OMA" id="CEPMYID"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070034">
        <property type="term" value="F:telomerase RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904868">
        <property type="term" value="P:telomerase catalytic core complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014886">
        <property type="entry name" value="La_xRRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045537">
        <property type="entry name" value="Lar7_xRRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19977">
        <property type="entry name" value="xRRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51939">
        <property type="entry name" value="XRRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="XRRM" evidence="4">
        <location>
            <begin position="285"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="229"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="367"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="416"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="372"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="416"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="432"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ80561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="442" mass="50456" checksum="624C349C49C50BA5" modified="2010-06-15" version="1">MFVPRQLQKKSLCQQQKESTPLAPTEKSTKNDYIDPTLAKEMVMTLEILFSDHGIESGPPEWFSARMRSVEGESDFIHLSALLDCPLLAEMKPKPSQMTLRKALTQYPSDFLQLSKDKYYIRRRQEYLASSRSPAGHPKCSEDSTIYIEPHVTGITLNPGRVARMLSQSAMPRKYLPVQFVEAGDTAWAFVILSAAVSHEDAENQGLWPKDWIIMTKHEWRKRDEEYQHLRGRRPTSPARIRLPERHDGASGSSVQQMWSDLPIFPTVPVDPASVREGDQKMNADYESGLIVYLTNIHPLTTKETITSFITRQVDRYNGKKRSSTLKDRDGPPVNINYVDYSKGLTTAYLRLRKAQDSELVVSALKKRKRRMQDGNDKKGKKVTNDSGRDNWVKATNVEGNEERIYWEKVLAAKWKGKGKNKRKLPSHDAGESSSKQIKFSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>D5G804</accession>
    <name>D5G804_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00002769001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430037">
        <property type="protein sequence ID" value="CAZ80647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836456.1">
        <property type="nucleotide sequence ID" value="XM_002836410.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G804"/>
    <dbReference type="STRING" id="656061.D5G804"/>
    <dbReference type="GeneID" id="9184120"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002769001"/>
    <dbReference type="HOGENOM" id="CLU_1176157_0_0_1"/>
    <dbReference type="InParanoid" id="D5G804"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="194"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="236" mass="25225" checksum="11C4F734E97103EC" modified="2010-06-15" version="1">MNSVMSPPATKPPRRRGPRGSRKHGNNTSGDQDVTNRQPTANHSSGTPRYTPSTAAPPSAPQRQLYIPPPRTPSIPKVQILKPSTAPPAPPPPPIAPAARTTLMIRERQPPPAPIQTRPPPETATIAVVDDDHGNDKSSLPASPPPLASPKPAPAPPPPPPFETPDHKSLIRDRQAQIAAYLSRWDALFALASAKDAHDAEPNSGKRLPWPIENNSVDGAGWRPWGEYLHTGHGGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>D5G859</accession>
    <name>D5G859_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00002843001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430040">
        <property type="protein sequence ID" value="CAZ80702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836511.1">
        <property type="nucleotide sequence ID" value="XM_002836465.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G859"/>
    <dbReference type="GeneID" id="9188304"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002843001"/>
    <dbReference type="eggNOG" id="ENOG502S1M0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_430837_0_0_1"/>
    <dbReference type="InParanoid" id="D5G859"/>
    <dbReference type="OMA" id="WAYVHLI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="101"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="204"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="487"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="527"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ80702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="631" mass="70098" checksum="CF4C86156AFF352F" modified="2010-06-15" version="1">MDYFKAPNPGQSAYFSPLAPNDPHEGRAPGYSRYSHDYPHDTPTRPSPAGHPESHEMMFSPGGYSPDGLQTKYSFLPRGHQQMLGRNYSASSQKWKLLAKVTARFIVSFAACAGLIAAFKVYEDKKVLSRIEKRVFNAIYVGISLILSMNIISSFKAMASVMRWKILAAGTFRLREVDLVLDLSSFQSVWELMRISVGRPARSLACFAWILLGVAVQIGVALLGLSYNMENDGVVQLTNGLVNITDMRSIFPAFHMDTSVEPDYGVQQSTAHLYGDMATFFPYAVPGSPEYEDYTLLELLGEESTEWRYYFRETYYTEKPGAAGLPTTKTNRSVTVTPYCEYYPIVEGQFAGVNDTNVTISIGNRNVTIDWMEDWGTAATTYVNPGGFVSDTEGCGPRCSRVYIFQWVDEKWDPPSETGSFFNCTINISQVENANPLQPFHSMPDLTAEMAAGAIGLEGFVDDSERQFVRYTSAASWWGQRMKSTDIAAVLVGKFAVGVIAAYDFYGPKIEAMGLQSRPGISLSVNWLYVSLTLGSILFAQFVCGIFVVYSANSVFCKDDSYLSTARLLRPLVERLGNSGSTSTGFEISQTFTRSVRYGVRSDLSTGQPIHHLDVGEDIMRVPRFPEGHYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>D5G863</accession>
    <name>D5G863_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00002905001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430041">
        <property type="protein sequence ID" value="CAZ80706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836515.1">
        <property type="nucleotide sequence ID" value="XM_002836469.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G863"/>
    <dbReference type="STRING" id="656061.D5G863"/>
    <dbReference type="GeneID" id="9188361"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002905001"/>
    <dbReference type="eggNOG" id="ENOG502QSRP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027977_2_0_1"/>
    <dbReference type="InParanoid" id="D5G863"/>
    <dbReference type="OMA" id="RGISWGH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009395">
        <property type="term" value="P:phospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007312">
        <property type="entry name" value="Phosphoesterase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31956:SF15">
        <property type="entry name" value="ACID PHOSPHATASE PHOA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31956">
        <property type="entry name" value="NON-SPECIFIC PHOSPHOLIPASE C4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04185">
        <property type="entry name" value="Phosphoesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="305" mass="34387" checksum="C7A78B8AED548570" modified="2010-06-15" version="1">MNKLPANISSVADLLEDKGISWGEYQEDMPYTGFTGNEYRNQRTQANAYVRKHNPLVMFNSVADAPERLGKIKNLTLFWSDLEAKNLPQWIFITPNMTSDGHDTSVTVAGAWTRSFLEPLLTNDYFMKDTLILVTFDENHTYTRPNRVVGILLGGTVPEGAHGTTDSNYYDHYSEISTIEANWDLHTLGRYDVGANVFSLVANKTKDVVRKHPNLDGVYLNESYPGILHSKKWAPQPVPNTSLNISGRSVLPDVVKVWGSDENQKQTVYGGDLEIPSAANPPKYPSPGAHYWYIMLTELWLYLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>D5G897</accession>
    <name>D5G897_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00002940001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430041">
        <property type="protein sequence ID" value="CAZ80740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836549.1">
        <property type="nucleotide sequence ID" value="XM_002836503.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G897"/>
    <dbReference type="STRING" id="656061.D5G897"/>
    <dbReference type="GeneID" id="9188342"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002940001"/>
    <dbReference type="HOGENOM" id="CLU_723680_0_0_1"/>
    <dbReference type="InParanoid" id="D5G897"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF129">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="52"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="132"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="166"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="256"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="7">
        <location>
            <begin position="70"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ80740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="369" mass="41074" checksum="054CDB99A976DC31" modified="2010-06-15" version="1">MGVITRLVEPTNIPGHAVRTPIDNFYLLFAPADFTHPAALDPEYIPDTRGSLLKGFVLASLAIATVAVGLRIYSRLRILKKLGWDDWAIIPALITYMAYTGLIIWGTESAGLAEHLYDLTARQILNWFKVQYLIVVVAPITFLLIRFSILLFLHRLAGNGEPGTQIAIKILHVLNFLWYPASVLPPVLMCAPREMRMWDLMAIIRAQCLQPKVADTMQINSTTMVVLSATFDIACLLVPVRLVWKLRMSARKKAVVSSLFGVGILACVCGILRAYFMARLLEKNGTSFDSTWNMVNPLICAQMEACLGIITACGPSLKMLYKQIHGTLESSIGKRSTTGEFSIIGAKRWSNRSEQRTEFSERVMSSLPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5G899</accession>
    <name>D5G899_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00002942001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430041">
        <property type="protein sequence ID" value="CAZ80742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836551.1">
        <property type="nucleotide sequence ID" value="XM_002836505.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G899"/>
    <dbReference type="GeneID" id="9188344"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002942001"/>
    <dbReference type="HOGENOM" id="CLU_2308117_0_0_1"/>
    <dbReference type="InParanoid" id="D5G899"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ80742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="100" mass="11824" checksum="C53B1A9852B4FDC3" modified="2010-06-15" version="1">MTNDPVPPLFFIPLVQLWDEMLRSYFLRKPLIYYYHIINHRVIAKLQLSPLKIKKILSYIGYRYYCTVQVQAFLAVKAIMIPTVQYRHTGWPGPEAVLVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8C3</accession>
    <name>D5G8C3_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 15">Alkaline phosphatase</fullName>
            <ecNumber evidence="3 15">3.1.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">GSTUM_00004737001</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a phosphate monoester + H2O = an alcohol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15017"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:67140"/>
            <dbReference type="EC" id="3.1.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="13">Binds 1 Mg(2+) ion.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="13">Binds 2 Zn(2+) ions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the alkaline phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.1" evidence="3 15"/>
    <dbReference type="EMBL" id="FN430042">
        <property type="protein sequence ID" value="CAZ80766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836575.1">
        <property type="nucleotide sequence ID" value="XM_002836529.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8C3"/>
    <dbReference type="FunCoup" id="D5G8C3">
        <property type="interactions" value="181"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G8C3"/>
    <dbReference type="GeneID" id="9188368"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004737001"/>
    <dbReference type="eggNOG" id="KOG4126">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008539_6_0_1"/>
    <dbReference type="InParanoid" id="D5G8C3"/>
    <dbReference type="OMA" id="KAAGYMT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004035">
        <property type="term" value="F:alkaline phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16012">
        <property type="entry name" value="ALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.60.40:FF:000002">
        <property type="entry name" value="Alkaline phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000063">
        <property type="entry name" value="Alkaline phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.60.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001952">
        <property type="entry name" value="Alkaline_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018299">
        <property type="entry name" value="Alkaline_phosphatase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11596">
        <property type="entry name" value="ALKALINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11596:SF5">
        <property type="entry name" value="ALKALINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00245">
        <property type="entry name" value="Alk_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00113">
        <property type="entry name" value="ALKPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00098">
        <property type="entry name" value="alkPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00123">
        <property type="entry name" value="ALKALINE_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 13 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8 13 15" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="35"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphoserine intermediate" evidence="12">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="323"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="332"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="371"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="372"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="477"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601952-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR601952-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAZ80766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="568" mass="62172" checksum="E6986644C7D32E16" modified="2010-06-15" version="1">MSRRSSTERSAEEDAALLSGRPTNKQAERGKWDGWVKLGLGVWAMAATAGIVVIAVLWVNNGSGLNSKFPPEGKRNIVFMVSDGMGPTSLALTRSYRQFSDSLPWNHTLTLDKHFIGTSRTRSSSSLVTDSAAGATAFSCGMKSYNGAISVLPDGSPCGTVLEAAKKAGYMTGLVVTTRITDATPACFSAHVNLRGEEDRIAEQQIGDYPLGRMVDLMLGGGRCHFLPNTADGSCRDDSRDLVSEAQKRFGFSYVDDRKSFDGLKKGAVKLPLLGLFAPTDIPYDIDRKDEDYPSLEEMTEVALKALEIATKNSDKGFFLMIEGSRIDHAGHGNDPAAQVHEVLAYDRAFKRVSDWIAGSRYPGVVVSTSDHETGGLAAARQLHEEYPEYLWYPEVLANASSSSEYLAAQYARYEGPADQEEDYIRDTILIKGLGIYDASDDEIEAIKDHKDDVASAWVLADVISRRAQVGWTTHGHSGVDVNIYGSTGTSKLKGNHENTEVGEFLRDYLQVDVDAITKELKGVNRVDAPGLDKAGYEWMGRPLSELKEAGFETDKLDCYHGQFRHKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8E1</accession>
    <name>D5G8E1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Structure-specific endonuclease subunit SLX4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00004773001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLX4 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430042">
        <property type="protein sequence ID" value="CAZ80784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836593.1">
        <property type="nucleotide sequence ID" value="XM_002836547.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8E1"/>
    <dbReference type="STRING" id="656061.D5G8E1"/>
    <dbReference type="GeneID" id="9186004"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004773001"/>
    <dbReference type="eggNOG" id="ENOG502S832">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005957_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8E1"/>
    <dbReference type="OMA" id="SICCLWK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033557">
        <property type="term" value="C:Slx1-Slx4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22999">
        <property type="entry name" value="SAP_SLX4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018574">
        <property type="entry name" value="Structure-sp_endonuc_su_Slx4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09494">
        <property type="entry name" value="Slx4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="335"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="510"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="710"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="721"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="783"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="810"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="835"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="849"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="866"/>
            <end position="877"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ80784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="958" mass="104691" checksum="A46C036EC113AD97" modified="2010-06-15" version="1">MVSSQPSSPLLSPSALLQELVEKGTAAIQKQNDTRTLLSRQVILENAWNLPNSDSRDGVVTKLPPKRKRALKPTGDPIAKISRARGNSITPTLGDFLAKSKVLGEAGAKNTTKPKIKNTRKKRATMNDGQIVAKTAKKSMPTKSPFFAPQDAAEVPSKPVIYGNPSSIPQSPTKLITEPPIPLVHREWTPVKDTVISIDSSPTLGGIETGEGKDTKGRGTLDFANMVSEMKLSSSYSRDGSALASLGGLNKSGNGLIKKRAIEIVNLPSNSNIASGEPRASKCKQPRKKTRTITGQATTQYREAKEVTSHSLLNYFALEDATSESAVVEKLTRKRKTASATKRDSSPAPKLLSPKAAKRQFDETECIFGSSSQLEVMAEIETVDNLEPAEDEDTPDYIPPSTSIVRRNFLKTLTKDRKGGNKLVGTRHWTNIGLLDVSTVEDFGSHSEEFPMEQPMLPESRLGRRTVSKLWDAAARDLDGGLLSVEVVDMSVDNGILSLPSNPGPVEVISTARSSSSSEGPALPDPMGTVEDEPVPNLRIEGHLTRPPSIVVQGEDMRNLQVEGQPANSPQKNGVGEEFIPHLRTEDMPPVKLKRFVIPDADDVRELSFDNFAKTSDRASSMIPKSVTTSVVDTPTSRVAALVNSSATTFNPDPQVQPEPSMPNYNAWITTKLKAEVARYGFKDLKTRSRMISILEECWLAKNKRNIEQDTNKGRGAAATKTLTQRTRSTSPTFTHLPQSGWPNISRSQEYSTTTAPGASKFRGNRPTIRRYPTESGVPGPPDRGRSPTRRDQSLTHQSMAKQFAVGNAPGTSIQYPRKSQSPSNSVQSTPPLSPKRKAKKHKQKQTVSPFSSPKSPSKSVSPSRSRSRSKSPKLSRKMISRIILDIEPGSEGCRFSHAILMYDPISVEELAEWLNARGIMEGGFGKHTVTTKDVKNWCISKGICCFSEDGWRVKRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8P1</accession>
    <name>D5G8P1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00003064001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CENP-T/CNN1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430049">
        <property type="protein sequence ID" value="CAZ80884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836693.1">
        <property type="nucleotide sequence ID" value="XM_002836647.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8P1"/>
    <dbReference type="STRING" id="656061.D5G8P1"/>
    <dbReference type="GeneID" id="9186252"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003064001"/>
    <dbReference type="eggNOG" id="ENOG502S8G5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024001_1_0_1"/>
    <dbReference type="InParanoid" id="D5G8P1"/>
    <dbReference type="OMA" id="AIRGYSN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051382">
        <property type="term" value="P:kinetochore assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22920">
        <property type="entry name" value="HFD_CENP-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028255">
        <property type="entry name" value="CENP-T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035425">
        <property type="entry name" value="CENP-T/H4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46904">
        <property type="entry name" value="CENTROMERE PROTEIN T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46904:SF1">
        <property type="entry name" value="CENTROMERE PROTEIN T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15511">
        <property type="entry name" value="CENP-T_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CENP-T/Histone H4 histone fold" evidence="7">
        <location>
            <begin position="403"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="258"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="377"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="502"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="55"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="133"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="145"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="271"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="521"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF15511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ80884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="532" mass="58415" checksum="B6A210A7FCB33262" modified="2010-06-15" version="1">MENRDDNSGGSGELPGITPRHSVAPGLTTEEADNETPQATLVQLAKVVNAPATPPQQTSTSAAAASQKKSVRRTPGAGRLAPLPSKRTARSPHVVRAFEQRRAVTPGRERRKSGRYPAARETPRDLLRALSRQLATTSTQYVPSPAQPAPRPAPRPSYSDRRASDVVFYSDPGHNSGDEDEELSPAPRLSMNLNEFEQAEEEEDARLPVPRYSLPLENIEDMDYTHTSIEFGRRAVTERPYRPSFGIRLSDRFADFHGVTGESSPRTAGYEDGDEREEEEDGETGVMGDESVASRMSVVNDGDGDVTGDITGALDGNLDLDGDEPSFFLPVPEEGGEGFAQDDMGESEDGTLLNGHAHEEEEEVEEEYDLPEVISAQEASSSTVPKPASAIKKRRKPLPLSRHGIPYPPFPRHTIKKMATKFSGSTISADTLDALVAASEAFFQQASEDLAAYSKHAGRKVIEDSDVIQLLRRQRQLNPQTTAFSLAQRYLPRELLQEIRMPVSKAQPRRRQRQRPSLQTVEEDGDEVVVDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8P9</accession>
    <name>D5G8P9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00003074001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLA2 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430049">
        <property type="protein sequence ID" value="CAZ80892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002836655.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8P9"/>
    <dbReference type="FunCoup" id="D5G8P9">
        <property type="interactions" value="226"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G8P9"/>
    <dbReference type="GeneID" id="9186259"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003074001"/>
    <dbReference type="eggNOG" id="KOG0980">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004601_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8P9"/>
    <dbReference type="OMA" id="FQMSVEM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:clathrin-coated vesicle"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035615">
        <property type="term" value="F:clathrin adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032051">
        <property type="term" value="F:clathrin light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043325">
        <property type="term" value="F:phosphatidylinositol-3,4-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080025">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048268">
        <property type="term" value="P:clathrin coat assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="ANTH_N_Sla2p"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cytoskeleton assembly control protein Sla2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cytoskeleton assembly control protein Sla2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1410.10">
        <property type="entry name" value="I/LWEQ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ANTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035964">
        <property type="entry name" value="I/LWEQ_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002558">
        <property type="entry name" value="ILWEQ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030224">
        <property type="entry name" value="Sla2_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10407">
        <property type="entry name" value="HUNTINGTIN INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10407:SF15">
        <property type="entry name" value="HUNTINGTIN INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07651">
        <property type="entry name" value="ANTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01608">
        <property type="entry name" value="I_LWEQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00307">
        <property type="entry name" value="ILWEQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109885">
        <property type="entry name" value="I/LWEQ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50945">
        <property type="entry name" value="I_LWEQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="7">
        <location>
            <begin position="11"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="I/LWEQ" evidence="8">
        <location>
            <begin position="816"/>
            <end position="1060"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="267"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="316"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="1019"/>
            <end position="1055"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="289"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ80892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1062" mass="120631" checksum="4B2EC3B6C71BF41D" modified="2010-06-15" version="1">MAYTGSTRNVDMSRSESDLVINIKKATNMEETAPKRKHVRSCIVYTWDHKSSQSFWAGMKVQPIMADEVQTFKALISIHKVLQEGHPVTIREAHAHTGWLESLTRGVAGEGLRGYGPLIREYIYFLLAKLAFHRHHPEFNGVFDYEEYISLKSINDPNEGYETITDLMTLQDQIDAFQKLVFSHFRHGVNNECRISCLVPLVQESYGIYKFITSMLRAMHQSTGDDDALAPLRDRYDGQHHRLVRFYYECSNLRYLTSLITVPKLPQDPPNLLSEEDAPPALPRRPANEMPPPPEPIPSPPAISQPEPEPISDFWSQEQLQQQRAYEEEQRRLQEQREAELRQQEMLALQNQRAYEEMQRQQQEQQRLAHEQLLRDQMARQAQGQAAELERQLLEYQGQLANNVLLLESYDQKVKALETELMNLNLNSQQQQQSKDDLIKSLQDQVAMWKSKYEALAKLYSQLRHEHLELLGRYKQMQLKANSAQEAVERREKLEREMKSKNLELADMIRERDRALYDLDRVKGAQKEEVEKVKRELRFAQEKADNNERSRGSELSALLTRHNREVADLEDILRGKQRMIDELSSRSDDKHNDMEQLLRAKEEELEIFKAGMDQTLLELSEMRHNNTETDHVLEAQIDELLLDQLKKLTEIIDSVLQSGVQRIDDALYELESPMNAGNQSASPGYLLSQIEKAQMSATEFATSFNHFIADGPNSTQSQIIKTLHVFAGAIADVLANAKGVTRFAPDERRSEAILSSARQSAGTAVKFFQNLMSFRLDDLSVDRKTDIVVSSNLEVQKHLQKLNKLAEQFMAKGGSDLSKASGDLGDVVDAEMNKAAAAIEAATARLAQLMKKPKNPGYSTFEISIHDSILDAAIAVTNAIAQLIKAATVSQQEIVAQGRGSSSRTAFYKRNNRWTEGLISAAKAVAMSTNMLIETADGVISGHKKLEQLIVVANDVAASTAQLVAASRVKASFMSKSQERLEAASKAVTSACKSLVRQVQDIISAKNREENEDVDYSKLTSHEFKVQEMEQQVEILQLETQLQQARQRLGEMRKHSYAQDQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5G909</accession>
    <name>D5G909_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00004931001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430053">
        <property type="protein sequence ID" value="CAZ81002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836811.1">
        <property type="nucleotide sequence ID" value="XM_002836765.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G909"/>
    <dbReference type="STRING" id="656061.D5G909"/>
    <dbReference type="GeneID" id="9181285"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004931001"/>
    <dbReference type="eggNOG" id="KOG1840">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_125_15_1"/>
    <dbReference type="InParanoid" id="D5G909"/>
    <dbReference type="OMA" id="VALCNER"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019894">
        <property type="term" value="F:kinesin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002151">
        <property type="entry name" value="Kinesin_light"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45783">
        <property type="entry name" value="KINESIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45783:SF3">
        <property type="entry name" value="KINESIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13374">
        <property type="entry name" value="TPR_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0802">TPR repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ81002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="228" mass="25762" checksum="AFC7108DAE678497" modified="2010-06-15" version="1">MALLDQKKFEEAVITYRRAVGSMERALGPDHPFTLESINMLGEILLDLRKYEEAEVMCRRALAGRERTLGLLHRDTLLSANNLGVALCNERKHYEAEVMYRRAFLGAEEMLGVDHQDTLLCIYNLAGALYKQGKYYEAEMMYWRALAGRERVLGPGHPHTLRCIIKLVAVLYDQKKFGAAETMYQRAVTEGEQALGPDYREGLKNTSCLAMFLQIRGLGTAGESVLPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>D5G923</accession>
    <name>D5G923_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">18S rRNA factor 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00004953001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in the small subunit (SSU) processome assembly and function, and in the 18S rRNA synthesis. Required for the early cleavages at sites A0, A1 and A2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ESF2/ABP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430053">
        <property type="protein sequence ID" value="CAZ81016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836825.1">
        <property type="nucleotide sequence ID" value="XM_002836779.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G923"/>
    <dbReference type="FunCoup" id="D5G923">
        <property type="interactions" value="888"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G923"/>
    <dbReference type="GeneID" id="9181299"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004953001"/>
    <dbReference type="eggNOG" id="KOG3152">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054086_0_1_1"/>
    <dbReference type="InParanoid" id="D5G923"/>
    <dbReference type="OMA" id="HMLSEQM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034462">
        <property type="term" value="P:small-subunit processome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12263">
        <property type="entry name" value="RRM_ABT1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039119">
        <property type="entry name" value="ABT1/Esf2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034353">
        <property type="entry name" value="ABT1/ESF2_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12311">
        <property type="entry name" value="ACTIVATOR OF BASAL TRANSCRIPTION 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12311:SF7">
        <property type="entry name" value="ACTIVATOR OF BASAL TRANSCRIPTION 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="277"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="249"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="36"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="58"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="309"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="319"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ81016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="341" mass="38939" checksum="4C5989DA20F4BF17" modified="2010-06-15" version="1">MSTKLEDNKKKQDWLVIEDSEEEGTGDDSEQEEEESRLKELERRGSKRYKVDHASPASEDDDDGEIDGGDSDASGEEDGKEIDGKQEGTREKGKKEGKNIDKHDDLLITSSLRLKPLSKEELAASKEATSKTGVVYLSRIPPFMKPMKVKLLLSRFGEIGRIFLSPEDPKSYARRVRFGGNKKRNFEEGWVEFKSKKVAKLVAETLNTTIVGGKKGSYYHDDVWNIKYLPKFKWHHLQAQIAYENASRQAKLRAEIAQATRENKTYIRNAERAKMVEKMESSKKRKLKELSGASGDNDSEQLEPLQIRRQFRQHSAKGRRAEEGEGRESSEKVKRLLSKVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>D5G949</accession>
    <name>D5G949_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="20 21">NADPH--cytochrome P450 reductase</fullName>
            <shortName evidence="20">CPR</shortName>
            <shortName evidence="20">P450R</shortName>
            <ecNumber evidence="20 21">1.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">cprA</name>
        <name evidence="24" type="ORF">GSTUM_00003136001</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24 25">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="20">This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. Involved in ergosterol biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19 20 21">
            <text>2 oxidized [cytochrome P450] + NADPH = 2 reduced [cytochrome P450] + NADP(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24040"/>
            <dbReference type="Rhea" id="RHEA-COMP:14627"/>
            <dbReference type="Rhea" id="RHEA-COMP:14628"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:55376"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <dbReference type="EC" id="1.6.2.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="20">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="20">Binds 1 FAD per monomer.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="20">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="20">Binds 1 FMN per monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="20">Endoplasmic reticulum membrane</location>
            <topology evidence="20">Single-pass membrane protein</topology>
            <orientation evidence="20">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="20">Mitochondrion outer membrane</location>
            <topology evidence="20">Single-pass membrane protein</topology>
            <orientation evidence="20">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="20">Cell membrane</location>
            <topology evidence="20">Single-pass membrane protein</topology>
            <orientation evidence="20">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="20">Belongs to the NADPH--cytochrome P450 reductase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="20 21">In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="20">In the N-terminal section; belongs to the flavodoxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.6.2.4" evidence="20 21"/>
    <dbReference type="EMBL" id="FN430055">
        <property type="protein sequence ID" value="CAZ81042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836851.1">
        <property type="nucleotide sequence ID" value="XM_002836805.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G949"/>
    <dbReference type="FunCoup" id="D5G949">
        <property type="interactions" value="772"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G949"/>
    <dbReference type="GeneID" id="9184345"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003136001"/>
    <dbReference type="eggNOG" id="KOG1158">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_17_3_1"/>
    <dbReference type="InParanoid" id="D5G949"/>
    <dbReference type="OMA" id="QKRYQRD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003958">
        <property type="term" value="F:NADPH-hemoprotein reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06204">
        <property type="entry name" value="CYPOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.990.10:FF:000009">
        <property type="entry name" value="NADPH--cytochrome P450 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000100">
        <property type="entry name" value="NADPH--cytochrome P450 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.360:FF:000024">
        <property type="entry name" value="NADPH--cytochrome P450 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000018">
        <property type="entry name" value="NADPH--cytochrome P450 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.990.10">
        <property type="entry name" value="NADPH-cytochrome p450 Reductase, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03212">
        <property type="entry name" value="NCPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003097">
        <property type="entry name" value="CysJ-like_FAD-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001094">
        <property type="entry name" value="Flavdoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023173">
        <property type="entry name" value="NADPH_Cyt_P450_Rdtase_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023208">
        <property type="entry name" value="P450R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384:SF17">
        <property type="entry name" value="NADPH--CYTOCHROME P450 REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384">
        <property type="entry name" value="NITRIC OXIDE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00667">
        <property type="entry name" value="FAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000208">
        <property type="entry name" value="P450R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00369">
        <property type="entry name" value="FLAVODOXIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 20" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 20 21" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 20" id="KW-0274">FAD</keyword>
    <keyword evidence="3 20" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4 20" id="KW-0288">FMN</keyword>
    <keyword evidence="2 20" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="14 20" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="16 20 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="15 20" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 20" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="9 20 21" id="KW-0521">NADP</keyword>
    <keyword evidence="12 20 21" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 20" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="18 20" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="13 20" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="17 20" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="22">
        <location>
            <begin position="70"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="23">
        <location>
            <begin position="276"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <begin position="76"/>
            <end position="81"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <begin position="127"/>
            <end position="130"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <begin position="168"/>
            <end position="177"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <begin position="451"/>
            <end position="454"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="523"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <begin position="585"/>
            <end position="586"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <begin position="591"/>
            <end position="595"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="627"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="665"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00049342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRNR" id="PIRNR000208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="CAZ81042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="666" mass="73587" checksum="59C1F98F2404806F" modified="2010-06-15" version="1">MATQLDTLDVLVLAALLLGTIAYFTKGSLWAVEKDTITGIYGGSKANGAATKGSKTRDIVKTMEESDKNMVIFYGSQTGTAEDYASRLAKEGSARFGLKTMIANLEEFDYENLDTLPEDKVAVFVMATYGEGEPTDNAVDFYEFIMGDDVNFTEGDRLGNLKFIGFGLGNNTYEHFNAVIRRLTATLLKLGAKQIGNIGEGDDGTGTMEEDFLAWKEPMWKALQEEMDLEEREAVYEPVFSIAERTELSVEDDTVYLGEPNKNHLEGGSKGPYGANNPFIAPIKSSREIFTVKDRNCLHMEIDLTGTNLSYTTGDHIAVWPTNAGKEVDGFLKVLGLEEKKDGVIDVTGIDSTAKVPFPTPTTYDAIARYHLEICAPVSRQFLASLAAFAPTPTAKDELTRLGGDKDYFQETISSRYLNIAQVLELNAPGEIWSNVPFSLMVEGLNRIQPRYYSISSSSLVQPKLVSITCHGDPEPDPHGLSYAITGPRNKYDGIHVPVHTRASNFRLPSDPSKPVIMIGPGTGVAPFRAFVQERAALAEKGERIGKTLLFFGCRKASEDFLYQEEWKEYASKLGDSFSLITAFSRDGPEKVYVQHRLKERAKEVNELLAQGSYFYVCGDAAHMAREVNTSLGSIIAQERGLTETQGEEIVKRLRSSNLYQEDVWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9L0</accession>
    <name>D5G9L0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00003368001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430062">
        <property type="protein sequence ID" value="CAZ81203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837012.1">
        <property type="nucleotide sequence ID" value="XM_002836966.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9L0"/>
    <dbReference type="STRING" id="656061.D5G9L0"/>
    <dbReference type="GeneID" id="9185340"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003368001"/>
    <dbReference type="eggNOG" id="ENOG502S9DV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_369697_0_0_1"/>
    <dbReference type="InParanoid" id="D5G9L0"/>
    <dbReference type="OMA" id="GDYWLPT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070823">
        <property type="term" value="C:HDA1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038609">
        <property type="entry name" value="HDA1_su2/3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021006">
        <property type="entry name" value="Hda2/3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11496">
        <property type="entry name" value="HDA2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="393"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="718"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="556"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="395"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="409"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="433"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="738"/>
            <end position="753"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ81203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="753" mass="82664" checksum="1A75A327F6F917B4" modified="2010-06-15" version="1">MSENTGKAPPAPSPRPRTVAEIREKLLLNRKATDEKIKLARNHRDALKAPVKAPRSASESLSLGNSPLALGSERAGSFSNNYSSPAMFGSPNLGAEEYLLGLPLGTDSSPNGVVAQRAAYISAITAKSEVIDKFLNHPFPAPEELAGEMDKLAAECRLIAVHPSLSCAETNLRDVDFERESKNYIMTSPKFLFLKKLFDSISELSLKIEIVASPGGTIDMLEIFFRGIKVHCTRRDKLSQLPRLSASEQRGFSKVALLPSSKSGAVPVNADLVIAMDSTFVADVPEIVNARKSLFVVGKLAPVLRLVSLNTLEHIELCYKDSEPQYPALYTYIETTKNLLHDTGALVDSYKPTFAAAVPNIAEWLSRNPIAGAPKLEGFPLLPIFERLAQVQSPELHDKRKREGESAEKQSSGQTPPASTIQTKRLRIAGPINSPTPSSSCQQMGTATPAKDPTPIQSTPPTELGAHLPGSAMQVDGDDVGTTDTADYGPPKSWVYITEEVVDKMAPADLKKTLTAGKQALQEWKNFALLTQDTVKKQRESFMILQAEKDGLVKTLSKAEAREKRLDSEVSRLREDLKQANLDIQQAKADLSSAADPLPIIGQLRDEKEQLRKKLESMDRREKVKETEFEFLREQYQNATSSGVELRKDLDQMKAENVLLERRAAATVLELHKAHVSSQLDNYKSQVANLQSKLLEREARLARMEERVSGVPVTRGRALPVDRAATTTPAGSRHPEPVSQNPTRTDRSTNPLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9W3</accession>
    <name>D5G9W3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00005100001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CSN12 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430064">
        <property type="protein sequence ID" value="CAZ81306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837115.1">
        <property type="nucleotide sequence ID" value="XM_002837069.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9W3"/>
    <dbReference type="FunCoup" id="D5G9W3">
        <property type="interactions" value="879"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G9W3"/>
    <dbReference type="GeneID" id="9188549"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005100001"/>
    <dbReference type="eggNOG" id="KOG2688">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031567_1_0_1"/>
    <dbReference type="InParanoid" id="D5G9W3"/>
    <dbReference type="OMA" id="INRMFTL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045114">
        <property type="entry name" value="Csn12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12732:SF0">
        <property type="entry name" value="PCI DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12732">
        <property type="entry name" value="UNCHARACTERIZED PROTEASOME COMPONENT REGION PCI-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00753">
        <property type="entry name" value="PAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="2">
        <location>
            <begin position="224"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ81306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="428" mass="49042" checksum="812EE895F1C2EFED" modified="2010-06-15" version="1">MTTLYKTFRRALTTKDGALLGSIFSPENPDRGTFAFSTNHERIRGDVEYEISGLTPLRDENTRAAWIDTLVCYWKIAYILERDDGADVSWAKAYEGQKDLLLVMVRGYTKGNWPHWTLPVMYQICLSLRTVAILSDDQARRLGKASEHLEDAARYINKAFTLCISDRAPIEESRKWGTYYIVNLMFKTYFKLNSVGLAKNILRVLPASLADMPPLEAFPKSHIVTFKYYCGVVSFLEEDYGNAQAHLTYALNHCKADSIRNKELILTYLIPTTMLTKHKLPTLELLSRFPRLATLFNPVCKTIKSGNLQAFDAALRHGEEEFVKRRIYLTLERTKDIAMRNLFRKVVLYQNEESRTKIPVRQFQIAMGLEADGDTVMRSTPERVEVEIEEVECLLANMIYKGLMKGYISRERQTVVLSGKEPFPGTVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAH8</accession>
    <name>D5GAH8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00003593001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430077">
        <property type="protein sequence ID" value="CAZ81521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837330.1">
        <property type="nucleotide sequence ID" value="XM_002837284.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAH8"/>
    <dbReference type="GeneID" id="9187461"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003593001"/>
    <dbReference type="HOGENOM" id="CLU_1367122_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAH8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072259" evidence="1">
        <location>
            <begin position="23"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="200" mass="21945" checksum="6558BF606EE9810E" modified="2010-06-15" version="1">MAFQKLAIPLILTLYCALLASASSLRRRQNCIVNPILYTSFDETLTLSVQNKSHPLHNRAIYYTPLGDSTTGPVEHALTVYRSSPLPPAAFRLTSQKLYHAPPLGVPEQIPVYTRPSGYSGLDEVYFPATLPSEEPKVLWSGYPGCDPDTNAPQLELGPDGNMKVCAKPQGDDWRIYVQSGSGQVEDCVLSVIKLVKNPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAQ4</accession>
    <name>D5GAQ4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00003734001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430085">
        <property type="protein sequence ID" value="CAZ81597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837406.1">
        <property type="nucleotide sequence ID" value="XM_002837360.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAQ4"/>
    <dbReference type="STRING" id="656061.D5GAQ4"/>
    <dbReference type="GeneID" id="9187764"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003734001"/>
    <dbReference type="eggNOG" id="ENOG502S5XA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030828_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAQ4"/>
    <dbReference type="OMA" id="EDVFEWC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033698">
        <property type="term" value="C:Rpd3L complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039602">
        <property type="entry name" value="Rxt2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013904">
        <property type="entry name" value="RXT2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28232">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATORY PROTEIN RXT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28232:SF1">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATORY PROTEIN RXT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08595">
        <property type="entry name" value="RXT2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcriptional regulatory protein RXT2 N-terminal" evidence="2">
        <location>
            <begin position="36"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="212"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="450"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="221"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="272"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="454"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="489"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ81597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="499" mass="54495" checksum="A7B7552C2F532255" modified="2010-06-15" version="1">MAAERQYLADTIANVKKVLAAQSDGSESDDSIAYTSNRGRKLKRKARFVHEGALNDAKGPRVYKKEIEFYGKRRKVIYQKEPLRKRNEDSESDVEEDSDDDTTSVSDDLDPYSEIRIDKILAPCENAADLPSHPSMSHVYTSRTLNDLLQQSLTKLCEEQAHTTKLKNLLTVFLGDDPFVNLEKMQWLDEDFAAIARERSDNLLRELGGPLLSARGSISNPTTTAPPSAPALVPQVPTAPAPAASTTTTSPSKPTTNGDLATAPPKDPIPPTTTITTTTITTTTTGSPASSENPPPLTATTAAEDMDLDPPLAAPLKTEPSTPPTPPPRRMATRSTQNSHPTTPPPISPHHHLQEIDPWFFPPTFRVDPDFGLPAPEAAETRHLLSSAVQHQDEFLRGLNKVREGLLRAERFRKYVWGWCRAMEGTREYLAAGGGGTEEGGVDAEVEAGATLSDGEDYYDPVEWGLEEPLEKGKEEEEENGGEVVSAGKKTRGRRTVGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAT2</accession>
    <name>D5GAT2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00005291001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430086">
        <property type="protein sequence ID" value="CAZ81625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837434.1">
        <property type="nucleotide sequence ID" value="XM_002837388.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAT2"/>
    <dbReference type="GeneID" id="9187792"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005291001"/>
    <dbReference type="HOGENOM" id="CLU_036162_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAT2"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006878">
        <property type="term" value="P:intracellular copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.430.10">
        <property type="entry name" value="Copper fist DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051763">
        <property type="entry name" value="Copper_Homeo_Regul"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001083">
        <property type="entry name" value="Cu_fist_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036395">
        <property type="entry name" value="Cu_fist_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28088:SF9">
        <property type="entry name" value="TRANSCRIPTION FACTOR GRISEA, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G13190)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28088">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR HAA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00649">
        <property type="entry name" value="Copper-fist"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01090">
        <property type="entry name" value="Copper-fist"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00412">
        <property type="entry name" value="Cu_FIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57879">
        <property type="entry name" value="Zinc domain conserved in yeast copper-regulated transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50073">
        <property type="entry name" value="COPPER_FIST_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0186">Copper</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Copper-fist" evidence="9">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="93"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="291"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="346"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="496"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="93"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="294"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="348"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ81625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="605" mass="62502" checksum="B851441794526F86" modified="2010-06-15" version="1">MRGHRSSKCNHTDRILVKVRKPGRPLSSCPHTLSSSSPGTFVHSSPGAISNGGSTATKTNTYGEENGRNSCSCEITSVAIPKLTSCVCGVSGSASKSHASCKTSKPPSCSEASPLPSPVSSQGSAVFSMNKVTGPRHRVQRKSVAGADGSTTRRASLIAGDTAMEFLWKAQQEVGSSDVGVEKGSKYGAIGTNLAGVKKRGDGRSGYTSGASCAGGPGNGFSLIDPKGPIQGREFPVAVGAAFSPSNGVPAPKPDIGNGMMPAGGWSSTLDLPFFGEVLPTDAPHMVKPGGGCCSSSRSAPQTPQTNFDQRPSTVPKVVASYCNGGAGRGGNFVAKLGSPPLPVPGSEFSTRTSPQYNPAYDTTNSLEESLPRPSGPSTVTLTARELAWVQYLRATGFSSPPPLSGAGFLPDLQMTSGGEEGCISHPYNPRTVEYVKGLRNFALDDQTISPFHSQSNSPTLPQSHLSGSVDEMGEAGDPIASVHSHTATTRTNINLIIQTPPPPPSPDRESSTGAPENGVSPSAFVLVDYQIGPCSEAETECKCGDGCICLNCLSHDKGYGVEMARNAQWDASGLNGAEAGIVTGPNWWGFGNNGPVEANSWNSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBW0</accession>
    <name>D5GBW0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00005612001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ81960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837769.1">
        <property type="nucleotide sequence ID" value="XM_002837723.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBW0"/>
    <dbReference type="GeneID" id="9182996"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005612001"/>
    <dbReference type="HOGENOM" id="CLU_2575586_0_0_1"/>
    <dbReference type="InParanoid" id="D5GBW0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ81960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="81" mass="8906" checksum="34CB3355912FA4A0" modified="2010-06-15" version="1">MCFSRYLGLWRTWCTLYDTFISVALGGLSGGGGRRGGWETLFGVTCFGITSVFPVKLRVFKSRISVTSHSSNRIILVLVLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBW5</accession>
    <name>D5GBW5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00005618001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ81965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837774.1">
        <property type="nucleotide sequence ID" value="XM_002837728.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBW5"/>
    <dbReference type="STRING" id="656061.D5GBW5"/>
    <dbReference type="GeneID" id="9185400"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005618001"/>
    <dbReference type="eggNOG" id="KOG0204">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1856745_0_0_1"/>
    <dbReference type="InParanoid" id="D5GBW5"/>
    <dbReference type="OMA" id="KWIICIV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093:SF369">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093">
        <property type="entry name" value="CATION TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase C-terminal" evidence="5">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ81965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="138" mass="15852" checksum="0CAC1A543C7E1DAA" modified="2010-06-15" version="1">MVFNTFVWMQIFNEFNNRRLDNKFNIFENVHKNYFFIVINAIMIGGQVLIIFVGGKAFSIVPINGVQWAICVIVASFALPWAVIVRCVPDEFVRKHWCNLVRVLSPPVRAVVKVIPKRNKRADYQSDKEKGKAGEAKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBZ0</accession>
    <name>D5GBZ0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00005716001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Fluoride channel required for the rapid expulsion of cytoplasmic fluoride.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>fluoride(in) = fluoride(out)</text>
            <dbReference type="Rhea" id="RHEA:76159"/>
            <dbReference type="ChEBI" id="CHEBI:17051"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:76160"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the fluoride channel Fluc/FEX (TC 1.A.43) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430098">
        <property type="protein sequence ID" value="CAZ82033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837842.1">
        <property type="nucleotide sequence ID" value="XM_002837796.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBZ0"/>
    <dbReference type="FunCoup" id="D5GBZ0">
        <property type="interactions" value="117"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GBZ0"/>
    <dbReference type="GeneID" id="9185469"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005716001"/>
    <dbReference type="eggNOG" id="ENOG502QT5F">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030507_1_0_1"/>
    <dbReference type="InParanoid" id="D5GBZ0"/>
    <dbReference type="OMA" id="CYDLQHV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903425">
        <property type="term" value="F:fluoride transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003691">
        <property type="entry name" value="FluC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28259">
        <property type="entry name" value="FLUORIDE EXPORT PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28259:SF1">
        <property type="entry name" value="FLUORIDE EXPORT PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02537">
        <property type="entry name" value="CRCB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="120"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="163"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="231"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="260"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="292"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="323"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ82033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="370" mass="39949" checksum="630E4EAA801534AB" modified="2010-06-15" version="1">MSNPHPATAGQTETIDPPQLERQLSARIEDLPSHLVPPPVSTLQKPAPPQLSHFVQLHSYLIIFSILGTLSRIALVSITTYEATPLPPLFWPQFAGSLIMGFFLEDKTLFPASTKSTTSLYLGLTTGYCGSVTTFSSFIIGAFEQLAIAAPIHPSRRIGDNIAAMLAYVIGTIAISLGGLQVGTHLAVFSKGFSRELHFRWMDVLAAPIGFGLWVASVIMAVFIRRWRGDVLFACVFSPPGAIMRFWASRLGNPLRKAFPPGTFMVNMLGTAMLAGLLAGRHAHFGGNTCDVLRGLGDGFCGCLTTVSTFVVEVRGLRISHGYVYAGASIVVGLCLTILILGSYAGMGSWNRKTGMLETKRSMDYKYSAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GC17</accession>
    <name>D5GC17_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00005769001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430098">
        <property type="protein sequence ID" value="CAZ82060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837869.1">
        <property type="nucleotide sequence ID" value="XM_002837823.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GC17"/>
    <dbReference type="GeneID" id="9181329"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005769001"/>
    <dbReference type="HOGENOM" id="CLU_2741885_0_0_1"/>
    <dbReference type="InParanoid" id="D5GC17"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ82060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="71" mass="7775" checksum="259D0F641D99D51A" modified="2010-06-15" version="1">MHLPDATLIIPLYASLPAVVNVPKQTSDSPFIYSLKGDLVDGVQPVNLTWNATTKIVHHNHKPSQGRSEES</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCB0</accession>
    <name>D5GCB0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00000660001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430108">
        <property type="protein sequence ID" value="CAZ82153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837962.1">
        <property type="nucleotide sequence ID" value="XM_002837916.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCB0"/>
    <dbReference type="STRING" id="656061.D5GCB0"/>
    <dbReference type="GeneID" id="9184831"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000660001"/>
    <dbReference type="eggNOG" id="ENOG502QPM1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013967_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCB0"/>
    <dbReference type="OMA" id="IHGPASF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07389">
        <property type="entry name" value="MPP_PhoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.70">
        <property type="entry name" value="PhoD-like phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018946">
        <property type="entry name" value="PhoD-like_MPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038607">
        <property type="entry name" value="PhoD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052900">
        <property type="entry name" value="Phospholipid_Metab_Enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43606:SF2">
        <property type="entry name" value="ALKALINE PHOSPHATASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_5G03860)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43606">
        <property type="entry name" value="PHOSPHATASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G08710)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09423">
        <property type="entry name" value="PhoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="PhoD-like phosphatase metallophosphatase" evidence="2">
        <location>
            <begin position="284"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ82153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="605" mass="68418" checksum="02CBE331DAB0F7DA" modified="2010-06-15" version="1">MDSAPVFTALCALSSIIVRVGSYVFLRWMPGHVFPTAIFTAYGIYASLFAILYLGKPSYEVVASSSEINILPADHDAKTASRRAATGGRATRDTRKPTAIEATETTIYRRRQPHPLKHLLWGTPSVSGKLSLVTFAINTLLAAATWDLTFRTTYFYPSKNLSFARIGHVGPDSARVLLREPDSTRYPLSVWYSHGEDPTIDLHLVDTIPSLSEETDFTGTLAIHHLTPETKYRWYTSSNYSGTFTTAPRQNEAPKGGRFTFLTTSCIKANFPYDPTRHPLAVKGFRDLSGWVEKLEARFMLFLGDFIYIDVPRRPGLDKESYRQQYRQIYASPDWPSVGANLPWIHVIDDHEIANDWSSNTTGVYPAAIDPFNIYQHSVNPPPVRPAGDPYYTFDYGSAASFFMLDTRTYRSPVHSPDGPEKTMLGARQKADLLHWLSQGSGWKIIASSVPFTKNWRVNGRDTWAGYLYERQELLQQMWRIGGGRVVILSGDRHEFAATAFPPPPDSGYSIASTVHEFSCSPLNQFFVPIRTYKQLDNEDLLIKYVPDGNRKFGAITVDTTNEEQGVLKYRLVVDGSEKWSWVLTAPRVVEERLHKEKERGKWWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCB5</accession>
    <name>D5GCB5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00005798001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430109">
        <property type="protein sequence ID" value="CAZ82158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837967.1">
        <property type="nucleotide sequence ID" value="XM_002837921.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCB5"/>
    <dbReference type="GeneID" id="9186422"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005798001"/>
    <dbReference type="eggNOG" id="ENOG502S984">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_305923_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCB5"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="446"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="671"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="790"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="878"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="927"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="446"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="496"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="678"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="944"/>
            <end position="969"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="969" mass="108360" checksum="C63465FE559D7E89" modified="2010-06-15" version="1">MFARKLQLPLLNVTDADTLVFISPNTSPNPPHRVSSKALLASGSAVFRELLGPTSQHRALRKRRLVNVLPQGIKYVIDLSPPEEGPEALDAVLKLYCPKVVLEWQSGVPNGAYLEDDDHQYQRELWQVNSERRIADLHKHGTWETTEENCDSISDVDLSEVKVTRRGSGTGDAGVELMKGEWIEPTPPAAHSTRRHSAAIERILHIIHGLDPQLCTTPMWYTVCKVAVLLNCESAVVDHATSWLYADGKFIGKYPEIVMEIANDLKSKVLFKDAFALLVGKMLRANESDSEQDARCFDRIPEAALPWKRNIMHACWSLKARVKKAHDHLMSVREWLYNPIWISEYEKLARVLSIPNLDHEVYEASIALRDAITDAVFDRLAPVLRSPYLSGGRLRYDSLLFTRPFWQELGGLSADCFWEMFDENVYNAQNLWQECVRRYNVRRIKGKHPHPKRAQPPKRISDLSTARGVIGRGETPGDELSRTGDSFPVLPPRRVASGDERRPDRPDSQTLPDDFNALDFQQDEDIHPPGYEPSSEPPPSYTHIDEVSEDLVAKSTPRGIRIEIANPIKRKSMSGSPWDGEVVEGVASREEINSVTPRIGNSSKRRCSLEKSGDEKASTCSVSTVAGNEDYGEPEVFHISGVHEAIDGQMAPLNYDRKGKGKAVDFEHVQEPPVAEEPQSSSLQVHPNSTGSVAEGDLPIWLGAWAKEAEGSHDPLLGPLLDERELFQDLFKYNGGGNPESSSAAGVGVHATLDYTLYDCTTAQNPNRTQDYDRDYNPFTDLDLKRYAGNWGSGQNMDQESPPRDPPEISSSQHIALDIIWKQSLSYVHFISSPFTEIPTMYDPTSWPDDNFLLCLSDAEWKFLPLWTDGLDDGTGGVFGDPQPQPVPQSPPEECGEGTEVESLADSNYSDCLLDMVSLASFEARVFSEDEGEDMDSARSISTVEDEDEDEEWDGDSDDTEELGWEECF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCE0</accession>
    <name>D5GCE0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 16">Protein transport protein SEC23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">GSTUM_00005843001</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15 16">Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 16">Cytoplasmic vesicle</location>
            <location evidence="1 16">COPII-coated vesicle membrane</location>
            <topology evidence="1 16">Peripheral membrane protein</topology>
            <orientation evidence="1 16">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 16">Endoplasmic reticulum membrane</location>
            <topology evidence="2 16">Peripheral membrane protein</topology>
            <orientation evidence="2 16">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Golgi apparatus membrane</location>
            <topology evidence="16">Peripheral membrane protein</topology>
            <orientation evidence="16">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the SEC23/SEC24 family. SEC23 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430109">
        <property type="protein sequence ID" value="CAZ82183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837992.1">
        <property type="nucleotide sequence ID" value="XM_002837946.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCE0"/>
    <dbReference type="FunCoup" id="D5GCE0">
        <property type="interactions" value="973"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GCE0"/>
    <dbReference type="GeneID" id="9186365"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005843001"/>
    <dbReference type="eggNOG" id="KOG1986">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008658_3_0_1"/>
    <dbReference type="InParanoid" id="D5GCE0"/>
    <dbReference type="OMA" id="FPPHYAE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01478">
        <property type="entry name" value="Sec23-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11287">
        <property type="entry name" value="Sec23_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.730:FF:000001">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.380:FF:000001">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000006">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000008">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1670">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.730">
        <property type="entry name" value="Sec23/Sec24 helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012990">
        <property type="entry name" value="Beta-sandwich_Sec23_24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007123">
        <property type="entry name" value="Gelsolin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036180">
        <property type="entry name" value="Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037364">
        <property type="entry name" value="Sec23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006900">
        <property type="entry name" value="Sec23/24_helical_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036175">
        <property type="entry name" value="Sec23/24_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006896">
        <property type="entry name" value="Sec23/24_trunk_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037550">
        <property type="entry name" value="Sec23_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006895">
        <property type="entry name" value="Znf_Sec23_Sec24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036174">
        <property type="entry name" value="Znf_Sec23_Sec24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11141">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11141:SF0">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00626">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08033">
        <property type="entry name" value="Sec23_BS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04815">
        <property type="entry name" value="Sec23_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04811">
        <property type="entry name" value="Sec23_trunk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04810">
        <property type="entry name" value="zf-Sec23_Sec24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82754">
        <property type="entry name" value="C-terminal, gelsolin-like domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81811">
        <property type="entry name" value="Helical domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82919">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 16" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14 16" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8 16" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 16" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="12 16" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 16" id="KW-0653">Protein transport</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 16" id="KW-0813">Transport</keyword>
    <keyword evidence="9 16" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zinc finger Sec23/Sec24-type" evidence="18">
        <location>
            <begin position="59"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 trunk" evidence="19">
        <location>
            <begin position="126"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 beta-sandwich" evidence="21">
        <location>
            <begin position="405"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 helical" evidence="20">
        <location>
            <begin position="523"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="17">
        <location>
            <begin position="635"/>
            <end position="723"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00025471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU365030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF04811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF04815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF08033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="CAZ82183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="771" mass="86279" checksum="A9BD3372C2A45C5E" modified="2010-06-15" version="1">MDMEQLKDQWVVAEEQDGVRLSWNVFPSNRLESSRLVVPIGVLYTPLKEKEQPPVQYEPVTCKAPCRAVLNPYCQVDSRAKLWICPFCLQRNQLPPHYKDIGPNSWPLELSPAFSTIEYKLARPAPAPPIFLFVVDTCQEEDSLKALKDSLIMSLSLLPPHALVGLITYGTMAHVHELGYTECAKSYVFRGNKEYSAKQVQEMLGLLAAGLRPQQAPMAPGRPQPHMPPSSRFLLPVQQCEFQLTNVLEQLQKDPWPVANDKRPLRCTGVALSVAVGLLETSFANTGARIMLFAGGPGTEGPGIVVGPELREPIRSHHDIERDNIKYHKKATKFYENMARRTAHNGHIIDIFAGCLDQVGLLEMKSLANSTGGHMILTDSFTSSMFRQSFVKIFDKDTADNLLMGFNASLEVLTTKELKVTGLIGHAVSMNKKSTSVGETECGIGNTCSWKMCGLSPTTSVATYFEIASQAGPAQQQQGPQKGLIQYLTYYQHSSGQFHLRVTTIGRDLSGPTGDPSIAHSFDQETAAVLMARIAVFKAEVDDGPDVLRWVDRMLIRLCSRFADYRKDDPTSFRLEKNFTLYPQFVFHLRRSQFLQVFNNSPDETAFYRHVINREDVGNSLIMIQPTLDTYTFDEPPRPVLLDSTSIQPNHILLLDTFFHILIFHGETIAEWKKVGYQDQEGYENFKELLELPKEDARELIADRFPLPRFIVCDAGGSQARFLLSKLNPSTTHTSGGYGGVPQNAQTIFTDDVSLQTFMEHLMKLAVTGTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCS1</accession>
    <name>D5GCS1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00005994001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="FN430120">
        <property type="protein sequence ID" value="CAZ82314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838123.1">
        <property type="nucleotide sequence ID" value="XM_002838077.1"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GCS1"/>
    <dbReference type="GeneID" id="9187933"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005994001"/>
    <dbReference type="eggNOG" id="KOG0519">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_34_2_1"/>
    <dbReference type="InParanoid" id="D5GCS1"/>
    <dbReference type="OMA" id="KIEHGSF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16922">
        <property type="entry name" value="HATPase_EvgS-ArcB-TorS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000010">
        <property type="entry name" value="Sensor histidine kinase RcsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.130:FF:000002">
        <property type="entry name" value="Two-component osmosensing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041664">
        <property type="entry name" value="AAA_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339:SF5">
        <property type="entry name" value="HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13191">
        <property type="entry name" value="AAA_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01590">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25503">
        <property type="entry name" value="TPR_CHK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55781">
        <property type="entry name" value="GAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="1"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="12">
        <location>
            <begin position="1440"/>
            <end position="1662"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="13">
        <location>
            <begin position="1820"/>
            <end position="1942"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1215"/>
            <end position="1246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1215"/>
            <end position="1227"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="9">
        <location>
            <position position="1874"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ82314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1953" mass="216652" checksum="01E6DC26A1858E8C" modified="2010-06-15" version="1">MPVSPRPVVAKPRTQSDLIKILSVFSDVVHILAVAHQAGITHNNVNTHSIIVTKKPKTTGAGVPVLGKLGGWHLASRLEREDPGRAEGTMLRGLNPAPLQYIAPECTGRMNRSVDYRADFYSLGVALYELIVGFLPFRSADPLELIHQHIAQQPVPPTDVNASIPTAVSAVVMKLLAKNAEDRYQIASGLKADIDMLIRRMTEGRSLEGMRVGELDTTSQFVITEKLYGREEAVSMLKGAYEKCLNQGGCSMVMVRGGSGVGKSRLVNEIQRPVAENRGYFTSGKFDQYKRGFSFFTLVQTLQDLVRQVLSESIQSLSRWRTETIRALDGDAAVLVDPLANLGPVERENRFREVIGRFLAVFGRRGLVVFLDDLQWCSQSECADNGPTVVEHTGLLIIGTYRDSEIDQDHPLPPMLEYVQTRAGVDVVDIELGPLDQDSVRKIIGDTLHRDPDYGRLKEDAEMQTLTELVYAKTCGNAFFVMQLLKSLHRGGHIVFDFGAKGGGQWRFNLTSIEADDLPPTVVDLLVKQMLKLNGPTRTAMMLAACIGTDNVGLQSLAVAAGKKPEEMASDLWGGLDAGLLLPTSGNYKIPLALDEDSSMLGGCAVTYRFLHDRVQQAAYSLIPKNERAGVHRMIGTRLLKKVPEEDLDGMLYEIVNQLNHWLSPLEKDERHTLMELNLRAGKTAIAATAFDAALLYFLTAKQLLDDHEKEEEVDVGLEINLSLIEGYFAHQKYVESINLADAVLPKCIKPRDKIRCLVHKMSCLLVQGKLNETIETGLLGLGVLSLEVPLDDAKAKSHADMMRPRILMDVAQIKALEKMHRLKDENLILLQELISSLLLPIYMSRPALLEAVCFTSVAISLEFGISTAGAYPMLMTGVILSAEGTQEAHLKSYAYGRLAINLIEKDTLMCPAAPAIYEVYAGHIGIFHCSMNEVLKSLHQAVTAGVALFNVDYTVFAMAEIPSFGMFGGENLSAVHSKMVATKPSIRKFKQLTGMWWLSFPLQFLLNLRGLGNPDPICFEGEELGDSKALSKLLSSESMTHIYLYHMYRLIIAVIYGRYDIAADLATHYCEPMSGGVTGTFYASLTYFYSSIAFLHLYDTISDEQKDLLDRNIKQIQIWSTTAKGTFLHKSVFLEAEFTRVKKPSQQLEILDKYDHAISLATSSGFVHDAAFISERCGSWLRAFSKRRAAPYIREAVRGYTAWGATNKAMELRKGSAEDLATKDPHDDDETSHASSRRENEPSSLGSELDFRTVLKASLVISEGMHLEEVIVKLMKSVLQTAGADYGVLILKEDGNLHVETVGLLDQVSILEHEPLNTRADLVPVSIVNIVASLGEQILKDSDDPKFDATYGRDSYFQNRRAKSVLCMPIQNQLKPMGVLYLENKFVNHAFTRQRQELLNVLCTQAAVTIDKARLYRQMELAKKAAEEATAEKSSFLANMSHEIRTPFNALLSCSIFLLDTGLSEQQREYVETIRNSAVLTLQIIDGILDFSKIEHGAIDLQVSPFSLRDCIESALQLVAEPAATKDLELAFRNKCSNVDTVHGDITRFRQCVINLIGNAVKFTQEGHILVTTEAKKLPNSELWSVEVAVQDTGIGIPDNARDRLFRAFSQVDTSTRRTYGGTGLGLAISRKLAEMMGGDIWFESEKGVGSTFHLTITAEVTGRTWCADKRLIGRKAIVADTHSLSSNILADELEVEGLTVTRTNTAEATLKQLHDHGIGYYEFALIDLSVDKTCLLADQVNKFDPRIRVIVMSRFGVNLPSIAQAYNIALSFVRPAPRSRYVQAIHDVMNPNKKKHIIPSKNQEMELLRSLARRHPLNILLAEDNLVNTRVALQHLKRMGYSAKHAKDGIEVLEMCEEAAERGDMFDVILMDIQMPRADGIETSLELRRRYVDEERPTIIALTANATANDRERCQQAGMSSHIAKPILPNDLATALMSTSPLQLSAVKRQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>D5GD41</accession>
    <name>D5GD41_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00000957001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430139">
        <property type="protein sequence ID" value="CAZ82434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838243.1">
        <property type="nucleotide sequence ID" value="XM_002838197.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GD41"/>
    <dbReference type="STRING" id="656061.D5GD41"/>
    <dbReference type="GeneID" id="9183898"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000957001"/>
    <dbReference type="eggNOG" id="ENOG502RZYG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032571_0_0_1"/>
    <dbReference type="InParanoid" id="D5GD41"/>
    <dbReference type="OMA" id="AHMEISY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.50.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36182">
        <property type="entry name" value="PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G10930)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36182:SF1">
        <property type="entry name" value="PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G10930)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072296" evidence="3">
        <location>
            <begin position="20"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="300"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="196"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="216"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ82434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="321" mass="32020" checksum="FA2E0BFF80E1551E" modified="2010-06-15" version="1">MRSTHILSLVCLTAVLTNGHMTMTEPKPLLDPKNPNTKNGDYDYSAPLSKSGTNFPCKGHHKVMGTPEGAPVATWAAGSKQSFTIGGGASHGGGSCQAAISEDSGGSWRVMQSYVGGCPAAGQSYSFTVPAEAKSGVVLFAWTWFNKVGNREMYMNCASITITGGTGAGLSSYPELFTANIGNGCATAEGSDVLFPNPGRNVINTSDKTGSPTGNCAGGGGSSSGSSDGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSGSSVDFEIIRFFRYRFFGLWLWLWFGLWLWLWLWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>D5GD44</accession>
    <name>D5GD44_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00000968001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430140">
        <property type="protein sequence ID" value="CAZ82437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838246.1">
        <property type="nucleotide sequence ID" value="XM_002838200.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GD44"/>
    <dbReference type="STRING" id="656061.D5GD44"/>
    <dbReference type="GeneID" id="9183901"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000968001"/>
    <dbReference type="HOGENOM" id="CLU_1171349_0_0_1"/>
    <dbReference type="InParanoid" id="D5GD44"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14688">
        <property type="entry name" value="bZIP_YAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052635">
        <property type="entry name" value="Sec_Metab_Biosynth_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39607:SF3">
        <property type="entry name" value="BZIP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39607">
        <property type="entry name" value="XANTHOCILLIN BIOSYNTHESIS CLUSTER TRANSCRIPTION FACTOR XANC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="2">
        <location>
            <begin position="34"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="127"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="174"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="199"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ82437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="237" mass="26306" checksum="2CA71C02DDA5443A" modified="2010-06-15" version="1">MSIRFGRSGNGYDSSEVMETEDDDWRKVTDLNERRKIQNRLAQRNYRRKLRQRLEELERQAGKNSGPSTSVSNKKRPKQPTSGAGTVSETSARGRANSGPRRGESSHAPHGVVDTEGRFSTIDSLLSPPSVSGESEDSSASASSRGIYTSDFSPMLLRPTARSSENTYPVWQNQSQLQYPEPPSYSSSQPYIASTSEYPPTSSVPAPSVSPAPTHQQLQAFEYEQYLSTQCPPNYQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>D5GD58</accession>
    <name>D5GD58_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00000995001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430141">
        <property type="protein sequence ID" value="CAZ82451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838260.1">
        <property type="nucleotide sequence ID" value="XM_002838214.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GD58"/>
    <dbReference type="GeneID" id="9183915"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000995001"/>
    <dbReference type="eggNOG" id="ENOG502RFFW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_305699_0_0_1"/>
    <dbReference type="InParanoid" id="D5GD58"/>
    <dbReference type="OMA" id="ICARCYV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="25"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="118"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="185"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="290"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="370"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="398"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="418"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="970" mass="109211" checksum="104A2EC954A7E747" modified="2010-06-15" version="1">MSHFSYKRYPAPASSRQPPYPPQQQYPAHIPQYGAPPYVPPPPPPPPTPPSQFPQSQFPPPGTQGATHNTQKRAPSVFERLNHRRGQSATSPPPVPPKILDPNNPSPYQYRQYPAPPSSVSSSSSSISSSSSRSSRRYGSSSSPNLAQPYLDGCPGRCGDRSQSLNLRLPPRGYKPPSVESAPQSPLSLDTLSPDSKHVLDENESYFPTLAPAVQARLRQQPVPPGSPPREESLPPHTRLRGGFLDGRFENEPSTAESRSWKEYPVPFCEGSDEGSGPDSLLESEHDTGPESSVCSSPGGSRLYTQTSPKTPPSGGQDFRRYPQTSGHPEPVATPRERRSYPWRAPTDSLPRQRESQPYRHSPGPRIGYQPPPIQPPPPLQARQSPQLRYQPSPPFPAATHATPPRQLPPQQVRRDLLPPPPREPLPMCPKRTPVSWGQWFQMLSVPNFDVCANCYYTHIYPTLFAEHFHRIEKAPNVEASCAFSTERVLHRIWPNALERGVLNGLRDYALMRANIPECRTVSGDPVNGLSSRWWRVIDGSMPRFRCCDACYQDMVMATGFSSYFAPANPQPEEEAWVCDLSFPFISGGMLSVSNYQDNIRKDQWDDFVSVVRFRHDVPPCPGPSMVKSGERKWYIPKRPIPGLAVCEACYCDNVIPHFEDKFTEAVASPQQTLTCGMAVLAIKMPWDSAVQKGSFEVWWEAVYALMTLPQCSEKGFTDQVARDWYSLRHQHENLENFKCCRSCYVSILRPLDLARYFDPIRFPLQNPTSTRACSLAPDGPRYGAYMKKLGEASNRRRFDIFLSYVIPRANIPPCRPKSFLARMKWYGTDEFVACEECYFEYVRDSPLNSQLTVHARYSPEPQACDLYSPRMKHIWEEACAKRDIKYFARAAIQREEIYCEVLERSKEINEEIAARQAGAGRLTLDSAGSGGDEGYRYGRHGSGASIMDAESAELYKELEALQRRWKSVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDA3</accession>
    <name>D5GDA3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="11">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00006121001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid/polyamine transporter 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430142">
        <property type="protein sequence ID" value="CAZ82496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838305.1">
        <property type="nucleotide sequence ID" value="XM_002838259.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDA3"/>
    <dbReference type="FunCoup" id="D5GDA3">
        <property type="interactions" value="304"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GDA3"/>
    <dbReference type="GeneID" id="9183960"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006121001"/>
    <dbReference type="eggNOG" id="KOG1303">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009646_8_1_1"/>
    <dbReference type="InParanoid" id="D5GDA3"/>
    <dbReference type="OMA" id="PMGIKYA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015186">
        <property type="term" value="F:L-glutamine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005290">
        <property type="term" value="F:L-histidine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015188">
        <property type="term" value="F:L-isoleucine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005302">
        <property type="term" value="F:L-tyrosine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090513">
        <property type="term" value="P:L-histidine transmembrane import into vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015824">
        <property type="term" value="P:proline transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF692">
        <property type="entry name" value="TRANSMEMBRANE AMINO ACID TRANSPORTER FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="219"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="243"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="293"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="328"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="353"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="393"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="435"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="470"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="533"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="558"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="591"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="10">
        <location>
            <begin position="212"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="100"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ82496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="619" mass="67107" checksum="9D66E7F6EBB61B02" modified="2010-06-15" version="1">MSYSAKNDEANLSSGSPTPTPGAYFPVHHDISPTSISPNVDIPPTGLRQRRSSLSLGLDAIRYAGGVNSLDNFARSWTRAAGYFEITPSRQSFVSVDPSRDEEEPASSSAIDDESYYDDAEVTRHLATRASLPTTGFGSYGRTPMYGSMNTGFPGARLDHTATRHAADLFVQKQTAGEVVVVDETLDKEREPLLVTTVTTESGKVEQVIVGQSTLPQTVFNSVNVLIGIGLLSLPLGLRYSGWLIGSIFLVCSALITNYTGKLLARCLDKSPNQSLVTYSDIAYIAYGHKSRICVSVLFSLELMAACVALVVLFSDSLNALFPQIDKFQWKIIAGFVLTPLSFLPLKVLSFSSILGILSTFSIVMIIFIDGWLKPSSPGSLREPMPTYLFPPSWWTIPLSFGLLMSPWGGHSVFPNIYKDMRHPKKYNKAVDITYIFTFVLDITLAVTGILMFGDGVLDEITSNILELSGYPAALSMAMVAFVAIIPLTKTPLNARPIITTLEIFAGVDPRAIALQGESVGTSGLTCGLLKSVIRIGVNASIVIIAILVPSFDRIMAFLGSALCFSICVILPMMFYLKIYGDEVPKRERRINQILIVVCTIVATTGTVAAFVPKESLGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDA8</accession>
    <name>D5GDA8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Elongator complex protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00006127001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the WD repeat ELP2 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430142">
        <property type="protein sequence ID" value="CAZ82501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838310.1">
        <property type="nucleotide sequence ID" value="XM_002838264.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDA8"/>
    <dbReference type="FunCoup" id="D5GDA8">
        <property type="interactions" value="1068"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GDA8"/>
    <dbReference type="GeneID" id="9183965"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006127001"/>
    <dbReference type="eggNOG" id="KOG1063">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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    <dbReference type="OMA" id="ENFRHIS"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="P:protein urmylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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        <property type="entry name" value="Elongator acetyltransferase complex subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037289">
        <property type="entry name" value="Elp2"/>
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    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
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        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
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        <property type="entry name" value="WD40_repeat_dom_sf"/>
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        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44111">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44111:SF1">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="7 11" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="53"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="210"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="297"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="678"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="530"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="540"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005881"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020267"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ82501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="816" mass="88843" checksum="3C7E665A902F92C9" modified="2010-06-15" version="1">MTEVSCSYISIGGNRNPAAADWSPSGLLAFGAGRCISLWNPLDPEYRGVFSTLKGHTDRVNVVRFLPGQSLAEDIIISGSVDQSLRIWQRLENSDSFSPCSVVEGVHKASVSAIATCPRHPLVFASASTDGIVAVFGLSILSQGKLDVKLLQVFSTAPKFYPLALALSVLPSSSSPSPPALILAVSGSASSVSIYLSSSLSSQFSHQATLSGHENWIRSLAFTPEDYSNPGSDLILASASQDKYIRLWRIHPGEELPPAAAEGESKIFGLSNRLSNKVHQLRAEDDGVWSVTFEALLMGHEDWIYTAAWRPQTADGLGLRLLSTSADNSLSIWEPEESSGIWLTTSRLGEISDYKGASTATGSAGGLWMGLWSPDGKAVVALGKTGSWRLWKHNPQEGRWVQSVGIGGHVKEVMGCSWEKAGGYLLSTGLDQTTRLSAEWIRGNGGPNSWHEFSRPQIHGYDINCIASLGESRFVSGADEKLLRIFDEPKTIAGLLESLCGIRKLSKEPMPDAANVPVLGLSNKAVDSSAVLPLPNGNAPEEENDESDEQQESAQSATSVIEGLHHPPLEDHLARHTLWPEREKLYGHGYEISAVSCSPDGKFIATACKASTIDHAVIRLFETETWQEIKPPLRSHSLTVHGMAFSTDGSRLLSVGRDRAWTVFEKREAGWEKLDMREKGHTRIIWDGKWAPVEDVFATASRDKSVKLWHKGDGGWVNRAALRFEVPVTALDFLGEVIEGKCWLAIGLEDGGLHVYHVRVGDWASPELLIKLDDRITPDRSVTQITWRPTRGQVGGRDVAVSSEDFSVRVYSISIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GE24</accession>
    <name>D5GE24_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GTP cyclohydrolase 1</fullName>
            <ecNumber evidence="3">3.5.4.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">GTP cyclohydrolase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00006340001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">GTP cyclohydrolase 1 is the first enzyme in the biosynthetic pathway leading to folic acid.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; 7,8-dihydroneopterin triphosphate biosynthesis; 7,8-dihydroneopterin triphosphate from GTP: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GTP cyclohydrolase I family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.16" evidence="3"/>
    <dbReference type="EMBL" id="FN430164">
        <property type="protein sequence ID" value="CAZ82767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838576.1">
        <property type="nucleotide sequence ID" value="XM_002838530.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GE24"/>
    <dbReference type="FunCoup" id="D5GE24">
        <property type="interactions" value="333"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GE24"/>
    <dbReference type="GeneID" id="9183099"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006340001"/>
    <dbReference type="eggNOG" id="KOG2698">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049768_1_0_1"/>
    <dbReference type="InParanoid" id="D5GE24"/>
    <dbReference type="OMA" id="CEHMCMS"/>
    <dbReference type="UniPathway" id="UPA00848">
        <property type="reaction ID" value="UER00151"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003934">
        <property type="term" value="F:GTP cyclohydrolase I activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00642">
        <property type="entry name" value="GTP_cyclohydro1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.286.10:FF:000003">
        <property type="entry name" value="GTP cyclohydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1130.10:FF:000012">
        <property type="entry name" value="GTP cyclohydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.286.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00223">
        <property type="entry name" value="FolE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043133">
        <property type="entry name" value="GTP-CH-I_C/QueF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043134">
        <property type="entry name" value="GTP-CH-I_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001474">
        <property type="entry name" value="GTP_CycHdrlase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018234">
        <property type="entry name" value="GTP_CycHdrlase_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020602">
        <property type="entry name" value="GTP_CycHdrlase_I_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00063">
        <property type="entry name" value="folE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006825">
        <property type="entry name" value="PRK09347.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006826">
        <property type="entry name" value="PRK09347.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11109:SF7">
        <property type="entry name" value="GTP CYCLOHYDROLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11109">
        <property type="entry name" value="GTP CYCLOHYDROLASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01227">
        <property type="entry name" value="GTP_cyclohydroI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55620">
        <property type="entry name" value="Tetrahydrobiopterin biosynthesis enzymes-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00859">
        <property type="entry name" value="GTP_CYCLOHYDROL_1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00860">
        <property type="entry name" value="GTP_CYCLOHYDROL_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="8" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GTP cyclohydrolase I" evidence="13">
        <location>
            <begin position="115"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="25"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="37"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00055676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ82767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="292" mass="32869" checksum="A972ED64C8D3C165" modified="2010-06-15" version="1">MPPAESNLPNASPIVPGNQQLIESDKLFASIKPRSRLPTQHDISPLQPQRSRNFGENRPYLAVDVITPEDSRPSTPHSLKSMVDFDGLSWPSLGTRERLEATPEEAAARLNKLSGAIKTILECIGEDPNREGLLDTPMRHAKAMLYFTKGYEENLKSIVNNAVFQEDHDELVIVRDIEVFSMCEHHLVPFTGKMHIGYIPNNKVLGLSKFARIAEMFARRLQLQERLTKQVAMAIEEILQPQGVAVVMESAHLCMVMRGVEKTTATTTTSSMLGIFRKSTRTREEFMHLVKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5GE43</accession>
    <name>D5GE43_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00006371001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430164">
        <property type="protein sequence ID" value="CAZ82786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838595.1">
        <property type="nucleotide sequence ID" value="XM_002838549.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GE43"/>
    <dbReference type="STRING" id="656061.D5GE43"/>
    <dbReference type="GeneID" id="9183118"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006371001"/>
    <dbReference type="eggNOG" id="ENOG502RY09">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024132_0_0_1"/>
    <dbReference type="InParanoid" id="D5GE43"/>
    <dbReference type="OMA" id="VWTCYRR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000228">
        <property type="term" value="C:nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1390">
        <property type="entry name" value="NDT80 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052605">
        <property type="entry name" value="Fungal_trans_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024061">
        <property type="entry name" value="NDT80_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037141">
        <property type="entry name" value="NDT80_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35144">
        <property type="entry name" value="MEIOSIS-SPECIFIC TRANSCRIPTION FACTOR NDT80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35144:SF2">
        <property type="entry name" value="MEIOSIS-SPECIFIC TRANSCRIPTION FACTOR NDT80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05224">
        <property type="entry name" value="NDT80_PhoG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51517">
        <property type="entry name" value="NDT80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NDT80" evidence="4">
        <location>
            <begin position="159"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="NDT80" evidence="2">
        <location>
            <begin position="159"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="83"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="318"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="418"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="454"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="23"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="52"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="125"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="140"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="182"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="345"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="418"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="472"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="509"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="519"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="535"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ82786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="690" mass="72991" checksum="4FDC3C2D437EF340" modified="2010-06-15" version="1">MSPKPEPLDTSSSAVQREHHPISHSSIQSLPIPAASNSRFGASLPALQDPRYSATSSSGYESSSPTSAASSIAYSPFTSVFQPTSYPTSASSTTFPSYSMSSEPRPRIPSIHSPTSGMGPHHRSSSGSTKSVSPSLGSSARANYATSTPHLSHHLPSRGPQSESLPSPHSPLTPASRALDSPTITVSPTAGSNLASPLPGQHDPTSNGLGVNSAGPPLGETQLIHSLTSADGHLVRPEIHARVDKGFFLSERDWTCYRRNYFSVICSYSLQPTTYSLPLYLRRTAGGPTEHVLAFAMCISAVVDAPGGKVVDLVQHTPKRDKGPQHKPNRIKLSPQPTGGMAFAASSSSPVLQQQVHSPQAGSLDREYSFPQQTQQQQHIAVFDRIQFKSATANNGKRRAAQQYYHLIVELFADVGSPSSSAASVSGHPSNPQGSDGGWVKIAYRISAPMVVRGRSPGHYADERRTSASSPGAGGGSGNGNGTHPSPTSGGSGFMSPDGVTPASGLGGSSSSMGMTGLQLHTTGISSMHSSPATSSIHNPSVSSASSSSHLEPPIDPILDAETASQLNSNFVGYQYFPSTIYESTTQPPPQNLENNYETHSYSNDRKSEHPLETNCQQFDSDVSSYIPASISSNDSLRKTVKEEYSANWLGGGASVGREQRFNHTSLYPKCGRFEPAETSRGYYPDLSAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5GE44</accession>
    <name>D5GE44_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00006373001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S54 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430164">
        <property type="protein sequence ID" value="CAZ82787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838596.1">
        <property type="nucleotide sequence ID" value="XM_002838550.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GE44"/>
    <dbReference type="GeneID" id="9183119"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006373001"/>
    <dbReference type="eggNOG" id="ENOG502SYNQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_790343_0_0_1"/>
    <dbReference type="InParanoid" id="D5GE44"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050925">
        <property type="entry name" value="Rhomboid_protease_S54"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43731:SF14">
        <property type="entry name" value="PRESENILIN-ASSOCIATED RHOMBOID-LIKE PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43731">
        <property type="entry name" value="RHOMBOID PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="50"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="177"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="247"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="278"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="310"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S54 rhomboid" evidence="9">
        <location>
            <begin position="138"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="19"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ82787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="351" mass="38406" checksum="1669734F940A9BFB" modified="2010-06-15" version="1">MPREGHEEVSPRSVDGAAAGHGGGGGGNGGRNEAEFGSSGSNEPEPGRRVVLLFISLTLPVFILSKINLLTPFLISARDLESGSIPTTSSLTKEQLNAYIRAHRPDLLRLQHISQATWANTTLTPIRFFPQSADPKPWHTMITYAFNHGGMLHFFFCYMGFKAFAPSLSFMYGDRRTAAAFLLSSAMAGAAITAWERFLNPWSKVSGQDLSAALHHRRFPATAVTVIDEDGRNHLERRLKDALSTNLGSSGGIVALGTIAAIVCPGMELNLMFIPYDFRIRTLMAALAVFDFGGAFVWDYGLGISHMGHLAGYVSGILLYTLWLRRVPFAFGPRMGERLRKAAMQRPPPPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GE47</accession>
    <name>D5GE47_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00006376001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430164">
        <property type="protein sequence ID" value="CAZ82790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838599.1">
        <property type="nucleotide sequence ID" value="XM_002838553.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GE47"/>
    <dbReference type="FunCoup" id="D5GE47">
        <property type="interactions" value="193"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GE47"/>
    <dbReference type="GeneID" id="9183122"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006376001"/>
    <dbReference type="eggNOG" id="KOG3183">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052358_2_0_1"/>
    <dbReference type="InParanoid" id="D5GE47"/>
    <dbReference type="OMA" id="RQYCLKH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1110.10">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035896">
        <property type="entry name" value="AN1-like_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057358">
        <property type="entry name" value="UBL_ZFAND1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000058">
        <property type="entry name" value="Znf_AN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14677">
        <property type="entry name" value="ARSENITE INDUCUBLE RNA ASSOCIATED PROTEIN AIP-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14677:SF40">
        <property type="entry name" value="CDC48-ASSOCIATED UBIQUITIN-LIKE_ZINC FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25327">
        <property type="entry name" value="UBL_ZFAND1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01428">
        <property type="entry name" value="zf-AN1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00154">
        <property type="entry name" value="ZnF_AN1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118310">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51039">
        <property type="entry name" value="ZF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="AN1-type" evidence="5">
        <location>
            <begin position="19"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ82790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="294" mass="32569" checksum="C64F2A8AD1181BFB" modified="2010-06-15" version="1">MSGKTNEMPAKTQGLGEVEAIGKHCDLPECHLLDFLPFKCESCGGTYCLDHRTETAHKCAHEGRWAATRREKDFSSSIAPTRPKIRSLLQTDCAHPKCKTIINTPRDPAVNCTTCRKQYCLSHRLQDQHDCDKVKPPPPTVATANEKAKSALSRFKAWTSSKSDKVIQSTRPGASQIAELNKLKRAAKGDDKVPVGKRVYVYVEAEARTTTSKLPRGEFYYSSEWTVGRVLDKAAQSLQVANLNNKADDDEKKLRVFHVEGGRVLSFGEKIGDAVGNGNTIVLLRGLQMPELME</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>D5GED8</accession>
    <name>D5GED8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00006437001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430175">
        <property type="protein sequence ID" value="CAZ82881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GED8"/>
    <dbReference type="STRING" id="656061.D5GED8"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006437001"/>
    <dbReference type="HOGENOM" id="CLU_973835_0_0_1"/>
    <dbReference type="InParanoid" id="D5GED8"/>
    <dbReference type="OMA" id="PTQVCVD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="242"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="286" mass="30882" checksum="E4152646541AB308" modified="2010-06-15" version="1">MCHTFSLSIFRHGIRRLIENRCIKLCNNTTPTPPPLPVCTATVTRVSERTIYTGSVTVTSARTVYLPTATATVTATRTATVFTNTIRTITSAISTSTIYRTTITSSLPLNFHSQTTTTTRTLTTTTTTISTSLSLTTLPPITTTLATVITTTRPLLPPETITSTLTWTTSSISTITSTIPVTITRTYTSPATATKTITSPPGAPTGTTTITRTERATQTVTRGSRETVVDDGDQIIVYDDQWVKVVVIFLLALLAWFVGVWLIWKECVCCAYVPPPPPAPPAEGGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEN7</accession>
    <name>D5GEN7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00001317001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430180">
        <property type="protein sequence ID" value="CAZ82980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838789.1">
        <property type="nucleotide sequence ID" value="XM_002838743.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEN7"/>
    <dbReference type="STRING" id="656061.D5GEN7"/>
    <dbReference type="GeneID" id="9184891"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001317001"/>
    <dbReference type="eggNOG" id="ENOG502QQSQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031135_1_0_1"/>
    <dbReference type="InParanoid" id="D5GEN7"/>
    <dbReference type="OMA" id="RDLYWWA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021369">
        <property type="entry name" value="DUF2985"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35872:SF1">
        <property type="entry name" value="ALPHA-L-RHAMNOSIDASE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35872">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G07110)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11204">
        <property type="entry name" value="DUF2985"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="168"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="219"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="292"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="324"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ82980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="394" mass="43914" checksum="D07F8ECBA270512F" modified="2010-06-15" version="1">MASPSEASPPAIAAATDPLPEVLPIRRTRTASITQSILDANPHYGVLAAAGEALARAPSLKDLRRSSVGSGNSRGRRGSAISSAATPRAETGLRAAAIDEEEAGRRSREIPPVRGMIDGIESKKQDGRRAEMTAQDGGEQEDEDAEHGWWQTTLNGLKAFWKFFTKPMGFFITIYMLNVVAWGGMLFLLLCNAAPAMCVPDCNDIDSPRRIWIEIDSQILNALFCVTGFGLVPWRFRDLYFLLRWRLFSDKRALHKLEVIHGGWFRGPSGVNTAHTTTGQVAPLTKSWKMDYVVWLYAWNTFFQCALSGFMWGMNRYNRPSWSTGLFVALAFIVAGMAGGQVWWETKQVKAVEGERTVEKVEIEGGRKEKVKDKGRKFWAREERNVVVVNRLTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFH9</accession>
    <name>D5GFH9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00006908001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430208">
        <property type="protein sequence ID" value="CAZ83272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839081.1">
        <property type="nucleotide sequence ID" value="XM_002839035.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFH9"/>
    <dbReference type="STRING" id="656061.D5GFH9"/>
    <dbReference type="GeneID" id="9188220"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006908001"/>
    <dbReference type="eggNOG" id="ENOG502QQEZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020826_1_2_1"/>
    <dbReference type="InParanoid" id="D5GFH9"/>
    <dbReference type="OMA" id="TAETWAH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47842:SF1">
        <property type="entry name" value="DUF676 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47842">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="355" mass="38651" checksum="52E75A5D6C02F3DD" modified="2010-06-15" version="1">MKKKTLLLCFIHGFKGDDDTFDRFPFDLKALVSHALPQLNIESLDSANGKLQTRVIDLEVAAGTQSPTIDPSVRTVLVTHSMGGIVAADTLLSIIDDQVVKSGHNNPEKFMFPYIQGILAFDTPYLGLSPAIFAHGADTNLKTASSAISQVSALASGLFATRAATEGSKALNQPPSNEDKGKEPETPGFWSQWGKAVSYAGAASVLAAGGAVAYFKRDELGEGWGWVSSHLEFVGVLMKGEELKERTERASSIEGIGFANLYTALGDRGKAKGERTFCILPQKGGKGWFRCTNTMAQDEVNAHVSMFHPKTNPWYFEMSQLAKELVVRWATNEWSYFASGDMRMSMNGRIPGRDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFY3</accession>
    <name>D5GFY3_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00001948001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DEAD box helicase family. DEAH subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="FN430227">
        <property type="protein sequence ID" value="CAZ83426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839235.1">
        <property type="nucleotide sequence ID" value="XM_002839189.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFY3"/>
    <dbReference type="FunCoup" id="D5GFY3">
        <property type="interactions" value="1172"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GFY3"/>
    <dbReference type="GeneID" id="9181771"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001948001"/>
    <dbReference type="eggNOG" id="KOG0926">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001832_0_4_1"/>
    <dbReference type="InParanoid" id="D5GFY3"/>
    <dbReference type="OMA" id="EAIHNNS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17982">
        <property type="entry name" value="DEXHc_DHX37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000637">
        <property type="entry name" value="ATP-dependent RNA helicase DHX37/DHR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000779">
        <property type="entry name" value="Probable ATP-dependent RNA helicase DHR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF99">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX37-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="408"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="610"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="264"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="53"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="264"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ83426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1170" mass="128461" checksum="FF47592AF95C8232" modified="2010-06-15" version="1">MPSKYKPTRDRKQRRTPKNPRNADDNTSEALPPAAAVSSDPNVEVMIPLTKAQKAEKRKEDLLAEARAENPGMSGKKLKRFNKYIENKLKKEEKVEIMAKLAAGASSMDTGLLRSTKELGRGRETKKERVRRAVREVKAGIAGEESRGVVFEERVIRDPAELEVVIPPAVEPEEVVNLGVEAKVEERAAEKPPIVPAVVVGSGLKRPLETDENGFPIIKRTKKSAQTRQERLTAVVRVVEGEVGGSGSEDGDDLVEGAIDGDSVEAEAEGGPSESEEGGEENESEEEDSDGGGGGSEEDSEGEEDGGDEGNKPVSRLRRGQSKKANAFKAWALAQRKQALHDGEPEHSMPNLLGLKPKVVAIPKMAPARPPPPPEGIKKASFVTVGRPIEIQAARLSLPVVAEEQSIMEAVNANDCVVICGETGSGKTTQVPQFLYEAGYGSPNTPTPGLIGVTQPRRVAAVSMANRVGEELGPGGKEKVSYQIRFEGTAGPSTAIKFMTDGVLLRELSEDFLLRRYSAVIIDEAHERSINTDILIGVMSRVLKLRKELAEKYGTKPLKLIIMSATLRVSDFTENQRLFPVAPPLLKVEARQHPVSIHFSRRTVIEYPGEIYKKVCKVHRRLPPGGILVFLTGQNEITHLLKRLRKTFPFKKNVESPLHVLPLYSLLPTKEQLKVFGEVPEGSRMCVLATNVAETSLTIPGIRYVVDCGRSKERNYDKTTGVQSFDIGWVSKASAAQRAGRSGRTGPGHCYRIYSSAVYERDFPEFAEPEILRMPIEGLVLQMRSMNIDTIANFPFPTAPDRPSLQKSERLLEYLGALDPGGALTELGRVMNFFPLSPRFSKILIIGQQHECLPYIIAIVAALSVGDVFIPEHQLDIHREADDEDAAGNAETALEDTQRAKGRKEYYRAHRAFSSLDPTSDALKLLSVVCAYEYEKDSTNFCERNFLRLKAMQETHKLRQQISKIIVANCPGVLSSFNPKLPPPSTAQVKALKQIIASGFIDQVAIRADLLPNSPYKLSQNSVKRVTEVPYMTLFASSAFQRPSDEGLSLDPAVYIHPSSVLASQSLYPEYLVYSELKKSASPTGKVRMKPLTSVTGAHLAALAKGTPLITYSKPLGHVPPKVLDDSMGRRRECWVVPRIGGAIGRSEVGWGLPARKVVQRKEMGRWVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>D5GG52</accession>
    <name>D5GG52_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00007200001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430242">
        <property type="protein sequence ID" value="CAZ83495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839304.1">
        <property type="nucleotide sequence ID" value="XM_002839258.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GG52"/>
    <dbReference type="STRING" id="656061.D5GG52"/>
    <dbReference type="GeneID" id="9184557"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007200001"/>
    <dbReference type="eggNOG" id="KOG1477">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026654_0_0_1"/>
    <dbReference type="InParanoid" id="D5GG52"/>
    <dbReference type="OMA" id="IGTACKP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd12910">
        <property type="entry name" value="SPRY_SSH4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035780">
        <property type="entry name" value="SPRY_Ssh4-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="SPRY" evidence="6">
        <location>
            <begin position="127"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ83495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="280" mass="29566" checksum="57044A87FD5DE61B" modified="2010-06-15" version="1">MADPPPPFTPSASAADLPPAPPAIRHDVSPTSNAPLALAESGFTFCDENPLVPPQHLTAASLEEISRGALTLVVPPYKAYLNHVTVASSPENGLPIVSTSSNCPDTALLSSLPLYAAGFHHPANTGSPKHIYFEVRLLRLPRRADKGAVAIGFAAKPYPPFRLPGWNRGSLAVHGDDGHRYCNNAFGGSEFMEAPWRVGETVGIGMTFGTGRVGGGGGEVWVERNGKRVGGWEITEEVDSIDSMRGGDPKIGLDGRWDVYAALGVFGGGIQVEVSKFESV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGC3</accession>
    <name>D5GGC3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00002024001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430262">
        <property type="protein sequence ID" value="CAZ83566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839375.1">
        <property type="nucleotide sequence ID" value="XM_002839329.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGC3"/>
    <dbReference type="GeneID" id="9183163"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002024001"/>
    <dbReference type="HOGENOM" id="CLU_3242469_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGC3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="43" mass="5320" checksum="157C1E43C3B99146" modified="2010-06-15" version="1">MIEGLTLSYMLWWFITIYNTYAICTRIYIYVYYWRVKSPLSSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGK4</accession>
    <name>D5GGK4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Pyruvate dehydrogenase E1 component subunit beta</fullName>
            <ecNumber evidence="10">1.2.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00007537001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + pyruvate + H(+) = N(6)-[(R)-S(8)-acetyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:19189"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10478"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83111"/>
            <dbReference type="EC" id="1.2.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.2.4.1" evidence="10"/>
    <dbReference type="EMBL" id="FN430275">
        <property type="protein sequence ID" value="CAZ83734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839543.1">
        <property type="nucleotide sequence ID" value="XM_002839497.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGK4"/>
    <dbReference type="FunCoup" id="D5GGK4">
        <property type="interactions" value="733"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GGK4"/>
    <dbReference type="GeneID" id="9181704"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007537001"/>
    <dbReference type="eggNOG" id="KOG0524">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012907_1_1_1"/>
    <dbReference type="InParanoid" id="D5GGK4"/>
    <dbReference type="OMA" id="WYANCPG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045254">
        <property type="term" value="C:pyruvate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004739">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006086">
        <property type="term" value="P:pyruvate decarboxylation to acetyl-CoA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07036">
        <property type="entry name" value="TPP_PYR_E1-PDHc-beta_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.920:FF:000001">
        <property type="entry name" value="Pyruvate dehydrogenase E1 beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000006">
        <property type="entry name" value="Pyruvate dehydrogenase E1 component subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027110">
        <property type="entry name" value="PDHB_mito-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006667">
        <property type="entry name" value="PRK09212.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008854">
        <property type="entry name" value="PRK11892.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11624">
        <property type="entry name" value="DEHYDROGENASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11624:SF96">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0630">Potassium</keyword>
    <keyword evidence="9 10" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="11">
        <location>
            <begin position="47"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ83734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="373" mass="40389" checksum="847DF805E53F05FE" modified="2010-06-15" version="1">MFVARNFLSRAAGNALRKPLVNIHRVTAFQALKAEQKNYSTGGTRDYTVREALNEALAEELERDEKVLIMGEEVAQYNGAYKVTKGLLDRFGEKRVIDSPITEHGFAGLGVGAALAGLSPVVEFMTWNFAMQAIDQIINSGAKTHYMSGGIQPCSITFRGPNGFASGVAAQHSQDYSAWYGSIPGLKVVTPWSAEDAKGLLKAAIRDPNPVVVLENELLYGQAFPMSEEAQKSDFVLPIGSAKIERVGKDVTLVALSRCVGQALTAAATLKKKYGVETEVINLRSVKPLDVETIVKSVKKTNHLIAIESGFPSFGVASEILALSMEYMFDFLDAPAQRITGAEVPTPYAIGLEQLSFPDEDLMVKKTAQLLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGU3</accession>
    <name>D5GGU3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="13">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00007515001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">May play a role in the regulation of membrane traffic through the trans-Golgi network.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Binds to ARF1 and ARF2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the ESCRT-0 complex composed of HSE1 and VPS27.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430275">
        <property type="protein sequence ID" value="CAZ83715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839524.1">
        <property type="nucleotide sequence ID" value="XM_002839478.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGU3"/>
    <dbReference type="FunCoup" id="D5GGU3">
        <property type="interactions" value="754"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GGU3"/>
    <dbReference type="GeneID" id="9181685"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007515001"/>
    <dbReference type="eggNOG" id="KOG1087">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017092_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGU3"/>
    <dbReference type="OMA" id="PDNYEPN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14235">
        <property type="entry name" value="GAT_GGA_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16998">
        <property type="entry name" value="VHS_GGA_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.160:FF:000003">
        <property type="entry name" value="VHS domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000008">
        <property type="entry name" value="VHS domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000024">
        <property type="entry name" value="VHS domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052653">
        <property type="entry name" value="ARF-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008152">
        <property type="entry name" value="Clathrin_a/b/g-adaptin_app_Ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013041">
        <property type="entry name" value="Clathrin_app_Ig-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008153">
        <property type="entry name" value="GAE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004152">
        <property type="entry name" value="GAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038425">
        <property type="entry name" value="GAT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002014">
        <property type="entry name" value="VHS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47180">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-BINDING PROTEIN GGA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47180:SF1">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-BINDING PROTEIN GGA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02883">
        <property type="entry name" value="Alpha_adaptinC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03127">
        <property type="entry name" value="GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00790">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00809">
        <property type="entry name" value="Alpha_adaptinC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00288">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49348">
        <property type="entry name" value="Clathrin adaptor appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50180">
        <property type="entry name" value="GAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50909">
        <property type="entry name" value="GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50179">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="VHS" evidence="10">
        <location>
            <begin position="26"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="GAT" evidence="12">
        <location>
            <begin position="189"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="GAE" evidence="11">
        <location>
            <begin position="502"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="413"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="465"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="413"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="465"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00053552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00065344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ83715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="624" mass="67951" checksum="248512172F2CB8C6" modified="2010-06-15" version="1">MAARDRFGAFPQEPNGSKLQRFIQSACDPVNYEPNLALNLEIADEINKRKGNAPREAAVAIVNYINHRNPNVSLLALNLLDICVKNCGYPFHLQISTKDFLNELVRRFPERPPARPGKVHLKILEAIEEWRGTICQTSRYKDDLGFIRDMHRLLSYKGYVFPEVRREDAAVLNPSETLKSAEEMEEEERAAQSAKLQELIRRGTPVDLQEANRLMKIMAGYDQTSRTDYRAKAAEEIGKLREKARLLEEMLGKVHKGETIGQQDTFEELASALKTAQPKIQKIIEEEGEDPDAVVKLLELNDVINTTVERYNFVKRGDLNSAKALPLMAGASPSAVPPPPAVELSLIDLEDSANGGPSASASGKPASLEEDLLGLSFQDSGSSSFGQGGGIALGFGANTGIPGPPLLSSTVEANTAGQQQRITPSTSPAPQVVRQPPALPNYFQNVSTPPPQPMAPGGFVSPPIQQTPAPAVQRNNGNSAGDDEDDWAFTSSLPPEQPAPLPVSQNELVILNSNLHILLDIHRPVTMPNGPILVKAKFSNNASQPIEDLTFQMAVTKALALRMEPQSSRHLEARQRAGVAQIIRVSGVPRGGGGNVKMRWKVSYRVGMGPLNEEQGVIEGLPVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHM1</accession>
    <name>D5GHM1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="11">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00008035001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430320">
        <property type="protein sequence ID" value="CAZ84051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839860.1">
        <property type="nucleotide sequence ID" value="XM_002839814.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHM1"/>
    <dbReference type="STRING" id="656061.D5GHM1"/>
    <dbReference type="GeneID" id="9183705"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008035001"/>
    <dbReference type="eggNOG" id="ENOG502SIF7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070647_2_1_1"/>
    <dbReference type="InParanoid" id="D5GHM1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21176">
        <property type="entry name" value="LPMO_auxiliary-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046936">
        <property type="entry name" value="BIM1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046530">
        <property type="entry name" value="BIM1-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34992:SF10">
        <property type="entry name" value="COPPER ACQUISITION FACTOR BIM1-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34992">
        <property type="entry name" value="HYPHAL ANASTAMOSIS-7 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20238">
        <property type="entry name" value="BIM1-like_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072086" evidence="9">
        <location>
            <begin position="27"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="221"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="Copper acquisition factor BIM1-like" evidence="10">
        <location>
            <begin position="31"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF20238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ84051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="240" mass="25720" checksum="511221805AA3607F" modified="2010-06-15" version="1">MYRVRAWLLSVLGILSVLMCGGGGRGGGLVNGHFTVIYPQQRGYSYNTQFHFPCIPPTTNRSQWPTSGGALSLIPIHAFALTTVNIALGSDIDENILSNPYNVSMVPLFNQTGANSTFCLRKIKIPKKIVGDVADGVNATIQVIQLTGTGAALYSCIDITFSDKEADRFGAQEVWDKYCINGTGMGGYRLGDKDPSRPNATVVLSAASSPGRFAASSSPSLALPFLALVWFYILGFSNII</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIE9</accession>
    <name>D5GIE9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00008456001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430326">
        <property type="protein sequence ID" value="CAZ84292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840101.1">
        <property type="nucleotide sequence ID" value="XM_002840055.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIE9"/>
    <dbReference type="STRING" id="656061.D5GIE9"/>
    <dbReference type="GeneID" id="9184650"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008456001"/>
    <dbReference type="eggNOG" id="KOG1550">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_36_7_1"/>
    <dbReference type="InParanoid" id="D5GIE9"/>
    <dbReference type="OMA" id="WIKKSAY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051726">
        <property type="entry name" value="Chitin_Synth_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46430:SF1">
        <property type="entry name" value="CHITIN SYNTHASE REGULATOR SKT5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46430">
        <property type="entry name" value="PROTEIN SKT5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08238">
        <property type="entry name" value="Sel1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="200"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="215"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ84292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="236" mass="26182" checksum="EC8BFF12637366FE" modified="2010-06-15" version="1">MTRLGMACLRSDLGLQGRYREGVKWLKRATDVADAQYPNAPYELGCMHETGCGDDVFQDESYAAQLFTQAAELGHADAYYRMGDAYEHGKLNCPRDAALSVHFYTGAAQRGHSMAMMALCAWYLVGAEPVLEKNEDEAYQWALKASEYGLPKAEYAVGYFTEMGIGCRRDPLEANVWYVKAAGHGDERAIARLKVIQEAASGGSPTKKDRKKKEKTGNRAVLEKSRDGEDKDCIIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIJ8</accession>
    <name>D5GIJ8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">glucan 1,3-beta-glucosidase</fullName>
            <ecNumber evidence="9">3.2.1.58</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00008530001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Successive hydrolysis of beta-D-glucose units from the non-reducing ends of (1-&gt;3)-beta-D-glucans, releasing alpha-glucose.</text>
            <dbReference type="EC" id="3.2.1.58"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.58" evidence="9"/>
    <dbReference type="EMBL" id="FN430327">
        <property type="protein sequence ID" value="CAZ84341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840150.1">
        <property type="nucleotide sequence ID" value="XM_002840104.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIJ8"/>
    <dbReference type="FunCoup" id="D5GIJ8">
        <property type="interactions" value="113"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GIJ8"/>
    <dbReference type="GeneID" id="9183275"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008530001"/>
    <dbReference type="eggNOG" id="ENOG502QPYU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004624_0_1_1"/>
    <dbReference type="InParanoid" id="D5GIJ8"/>
    <dbReference type="OMA" id="GWDMQDL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004338">
        <property type="term" value="F:glucan exo-1,3-beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009251">
        <property type="term" value="P:glucan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050386">
        <property type="entry name" value="Glycosyl_hydrolase_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31297:SF1">
        <property type="entry name" value="GLUCAN 1,3-BETA-GLUCOSIDASE I_II-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31297">
        <property type="entry name" value="GLUCAN ENDO-1,6-BETA-GLUCOSIDASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6 10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072766" description="glucan 1,3-beta-glucosidase" evidence="11">
        <location>
            <begin position="19"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="12">
        <location>
            <begin position="89"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00036824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ84341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="413" mass="45852" checksum="B5D11DE7EDFA2CD1" modified="2010-06-15" version="1">MHLKSHLLAASLVGGVQSSFLPWNQARGDLGTRAPTFDYGKAKVRGVNLGGWFVLEPWITPSFFEPFGGSVYKPLAARFNNNNKKSTFYSQEDFQQIAAAGLNHVRIPIGYWAIRPLPGDPYVQGQLKHLNNAINWAGNVGLKVWIDLHGAPGSQNGFDNSGKRDSIEWQQGDNVAHTVETIRELAQIYARSQYGNAVTAIELLNEPLGPNLDRGKIEQYWKDGWGAVRDFSDTGVVIGDAFFDTKSWNGVMTTGWDHVLMDTHHYQVFDVGQLQQSPQDHVNAACSFGRSLVGVDKWTVVGEWSAARTDCTKWLNGVGRGTRWEGTFSGGPRIGDCGNRIQGSAASYSAEEKANTRAFIEAQLDAYELVDGWFFWTWKSQGSPDWELRDLLANGLFPQPITSREGMLFLATF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIM1</accession>
    <name>D5GIM1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00008558001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430327">
        <property type="protein sequence ID" value="CAZ84364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840173.1">
        <property type="nucleotide sequence ID" value="XM_002840127.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIM1"/>
    <dbReference type="FunCoup" id="D5GIM1">
        <property type="interactions" value="389"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GIM1"/>
    <dbReference type="GeneID" id="9183298"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008558001"/>
    <dbReference type="eggNOG" id="KOG0251">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014248_1_0_1"/>
    <dbReference type="InParanoid" id="D5GIM1"/>
    <dbReference type="OMA" id="IESEQTH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005545">
        <property type="term" value="F:1-phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032050">
        <property type="term" value="F:clathrin heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048268">
        <property type="term" value="P:clathrin coat assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006900">
        <property type="term" value="P:vesicle budding from membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16988">
        <property type="entry name" value="ANTH_N_YAP180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.150:FF:000004">
        <property type="entry name" value="ENTH domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.150">
        <property type="entry name" value="ANTH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011417">
        <property type="entry name" value="ANTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014712">
        <property type="entry name" value="ANTH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045192">
        <property type="entry name" value="AP180-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951">
        <property type="entry name" value="CLATHRIN ASSEMBLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951:SF5">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-BINDING CLATHRIN ASSEMBLY PROTEIN LAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07651">
        <property type="entry name" value="ANTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="4">
        <location>
            <begin position="35"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="313"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="515"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="566"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="664"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="515"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="532"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="566"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="664"/>
            <end position="678"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ84364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="691" mass="76370" checksum="0F6D1BF31EE41AE8" modified="2010-06-15" version="1">MAASFEKSVKACVKLRSIVASGVVWFIVARGERWWNQTGVDSGQNLNNGATKVKLAAPKSKYVEHILIATHAGEAGIAEVFRALNNRVKDQTWTIVFKSLIIVHLMIREGEREVTLRYLRKHPRLITVSHYSDAQEQGRNIRHYSQYLLERARTYGDVRTDYVRSGEGRLRKLSIEKGLLREVECVQTQIRALLKCTFLDDDVDNEISLLAFRLLVMDLLVLFHVVNEGVINVLEHYFEMSRYDAERALEIYKTFTKQTADVVEYLQQARGVETATRLQIPNLKHAPTSLTSSLEEYLHDPDFDVNRRQYLAQQEAKKSGKPVPAAKPKSPPPGQQPKTSAAQRFPSPTPANPVPEQAQPKPVAPDLIDFFESIESEQTHMFSNQPTNGQQIQMPQPQNQFAQQAQVDSAFGAQMQQQSAPQQPFVAPPQQFPTQTGPTFATGYQAQNPYNQVAQQQPLPPIIQTNNLPAIQQTPQQIQTSFTGAGFGGYTPSDQNSVTAPLMSTVPQRFNQPIQQPNYQQPQHQPQQQQQSLSPAVSLSTGATDTNPFRQSMMMTGSVSTAPSLYATSPPVSAQPKSTNPFARQASPTNLSPTSPPSAYPISTQSTSAYPQAMPISAQPTVRSMPTGTNPFARSVSSAGTGLTVPVNATGTSVTNPFRQSIMAQQQQNGAAQGTMGAWEEPRNSASFRQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJ79</accession>
    <name>D5GJ79_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00008875001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the spermidine/spermine synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840381.1">
        <property type="nucleotide sequence ID" value="XM_002840335.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJ79"/>
    <dbReference type="FunCoup" id="D5GJ79">
        <property type="interactions" value="949"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJ79"/>
    <dbReference type="GeneID" id="9185940"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008875001"/>
    <dbReference type="eggNOG" id="KOG1562">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048199_1_0_1"/>
    <dbReference type="InParanoid" id="D5GJ79"/>
    <dbReference type="OMA" id="FLYHEMM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004766">
        <property type="term" value="F:spermidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008295">
        <property type="term" value="P:spermidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.140.10:FF:000001">
        <property type="entry name" value="SPE3p Spermidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000013">
        <property type="entry name" value="Spermidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.140.10">
        <property type="entry name" value="Spermidine synthase, tetramerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00198">
        <property type="entry name" value="Spermidine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030374">
        <property type="entry name" value="PABS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030373">
        <property type="entry name" value="PABS_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001045">
        <property type="entry name" value="Spermi_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030668">
        <property type="entry name" value="Spermi_synthase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035246">
        <property type="entry name" value="Spermidine_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037163">
        <property type="entry name" value="Spermidine_synt_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002010">
        <property type="entry name" value="PRK00811.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00417">
        <property type="entry name" value="speE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11558:SF11">
        <property type="entry name" value="SPERMIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11558">
        <property type="entry name" value="SPERMIDINE/SPERMINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17284">
        <property type="entry name" value="Spermine_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01564">
        <property type="entry name" value="Spermine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000502">
        <property type="entry name" value="Spermidine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01330">
        <property type="entry name" value="PABS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51006">
        <property type="entry name" value="PABS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PABS" evidence="5">
        <location>
            <begin position="11"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ84572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="280" mass="31939" checksum="C2078675E3E0F959" modified="2010-06-15" version="1">MSEAVHPTIQDGWFREVSEMWPGQAMTLRVKKVLHHEKSKYQDVLIFESTDYGNVLVLDNVIQCTERDEFAYQEMITHLAMNSHPNPKKVLVIGGGDGGVLREVVKHECAVIRLSKQYLPHMSKGFDHPKSKVHVGDGFKFLEDYKNEFDVIITDSSDPEGPAESLFQKPYFELLFGALKEGGVITTQAENQWLHLQLITDLKKSCREVFPVVEYAYTTIPTYPSGQIGFMVCSKDANRDVSKPLRTVSDEEEQKMYRYYNKRIHEASFVLPTFAAAALK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJ83</accession>
    <name>D5GJ83_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="12">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00008879001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs. Specifically modifies U47 in cytoplasmic tRNAs. Catalyzes the synthesis of dihydrouridine in some mRNAs, thereby affecting their translation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a 5,6-dihydrouridine in mRNA + NAD(+) = a uridine in mRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:69851"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:17789"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="8">
            <dbReference type="Rhea" id="RHEA:69853"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 5,6-dihydrouridine in mRNA + NADP(+) = a uridine in mRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:69855"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:17789"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="9">
            <dbReference type="Rhea" id="RHEA:69857"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840385.1">
        <property type="nucleotide sequence ID" value="XM_002840339.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJ83"/>
    <dbReference type="FunCoup" id="D5GJ83">
        <property type="interactions" value="801"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJ83"/>
    <dbReference type="GeneID" id="9185944"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008879001"/>
    <dbReference type="eggNOG" id="KOG2334">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013299_3_2_1"/>
    <dbReference type="InParanoid" id="D5GJ83"/>
    <dbReference type="OMA" id="DESYTMT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102264">
        <property type="term" value="F:tRNA-dihydrouridine20 synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02801">
        <property type="entry name" value="DUS_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035587">
        <property type="entry name" value="DUS-like_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052582">
        <property type="entry name" value="tRNA-DUS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018517">
        <property type="entry name" value="tRNA_hU_synthase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45936">
        <property type="entry name" value="TRNA-DIHYDROURIDINE(20) SYNTHASE [NAD(P)+]-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45936:SF1">
        <property type="entry name" value="TRNA-DIHYDROURIDINE(20) SYNTHASE [NAD(P)+]-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01207">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01136">
        <property type="entry name" value="UPF0034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="DUS-like FMN-binding" evidence="11">
        <location>
            <begin position="7"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="335"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00045934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ84576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="386" mass="41516" checksum="6CF4B64F17D38021" modified="2010-06-15" version="1">MSGKCVLAPMVRTGELPTRLLALKYGADLVWGPETVDKGIIGAQRVVNSKINCIDFVKPQTGGQYDEKVIFRTCPAIESGKLIFQLGTASPELAVEAAKIIVGDVAGIDVNSGCPKHFSIHSGMGAALLKNPDKLIDILTSLVTEVGVPNSVPISVKIRLLEPHTDTIGLVQRLCTTGISRVTVHCRTIPMRPREPAIRGVLSEIANVCHEAGIECFANGDVTSRAHAEELIEKHGVDGCMIARAAETNPSVFLSGEKLPWRDVAREYMKIAVEVDNHFSNIKFCLLHIIPGKTDLYPPISRSKTARQICEILEIPYEPPAPRVEETTVEKVGSKAVQKAKSSPTVKAAGAGVGVKHGGKKRKERGIGGHSKTIPEQELQQAAVVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJD7</accession>
    <name>D5GJD7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00008952001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430330">
        <property type="protein sequence ID" value="CAZ84630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840439.1">
        <property type="nucleotide sequence ID" value="XM_002840393.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJD7"/>
    <dbReference type="STRING" id="656061.D5GJD7"/>
    <dbReference type="GeneID" id="9182631"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008952001"/>
    <dbReference type="eggNOG" id="KOG0725">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_1_1"/>
    <dbReference type="InParanoid" id="D5GJD7"/>
    <dbReference type="OMA" id="KDANTMF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050664">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="Short chain dehydrogenase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008">
        <property type="entry name" value="BENZIL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008:SF4">
        <property type="entry name" value="CHAIN DEHYDROGENASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G08710)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketoreductase" evidence="4">
        <location>
            <begin position="82"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ84630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="333" mass="35824" checksum="5EA668836F34D586" modified="2010-06-15" version="1">MNSSFRISSGLARRLLQQCGVSAPRRLLPFGASLVTRHISSSPDTNVTPGLTPKATGKAFEGNGKVLLGSQKRLPEFNLVDRVVLISGGARGLGLTQAEALLEAGAKVYALDRLEKPSPGFYEAQARATNNLGTSLHYRQIDVRDEAKLHASVQAIAEENGRLDGLIAAAGINEEHTALEYTAADFNKMMEINVTGAFLTAQAAARQMVRFGKGGSIVMIASMSGTIANRGLICPAYNASKSAVLQLGRNLAAEWGPYGIRVNTISPGYIVTAMVEELFTKFPERREDWPKQNMLGRLSKPQEYRGAAVFLLSDASSFMTGADLIMDGGHHSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJI1</accession>
    <name>D5GJI1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00009027001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430332">
        <property type="protein sequence ID" value="CAZ84674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840483.1">
        <property type="nucleotide sequence ID" value="XM_002840437.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJI1"/>
    <dbReference type="FunCoup" id="D5GJI1">
        <property type="interactions" value="151"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJI1"/>
    <dbReference type="GeneID" id="9182675"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009027001"/>
    <dbReference type="eggNOG" id="ENOG502QQMM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003021_1_0_1"/>
    <dbReference type="InParanoid" id="D5GJI1"/>
    <dbReference type="OMA" id="TMHQKYN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd11887">
        <property type="entry name" value="SH3_Bbc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057402">
        <property type="entry name" value="AIM3_BBC1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035552">
        <property type="entry name" value="Mti1_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46026">
        <property type="entry name" value="RHO-TYPE GUANINE NUCLEOTIDE EXCHANGE FACTOR, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46026:SF1">
        <property type="entry name" value="RHO-TYPE GUANINE NUCLEOTIDE EXCHANGE FACTOR, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25459">
        <property type="entry name" value="AIM3_BBC1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="6"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="73"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="972"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="123"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="184"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="202"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="245"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="288"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="307"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="336"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="350"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="367"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="385"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="411"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="427"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="466"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="553"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="578"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="647"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="671"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="730"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="750"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="774"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="815"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="849"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="973"/>
            <end position="990"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ84674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1161" mass="126695" checksum="D4B44332D79E14D9" modified="2010-06-15" version="1">MANTSVPPFKVKALFDYNSPHDDDLNFPADEVITVTEEEDADWYYGEYHDKATGELRQGIFPRNFVERILVELPPRPTRSGGGRKKVTEEHQPEPPADYLVSSIAAPKASETQTAIRQEKLEAAQPASSPPVAKAAPAFKAPPKQTPAPSAEKSAPSSSKPPPLEKPSSFKDRLALFNKGSAAPPVPFNPHKPPVNFIKKPFVPPPPSKHAYVPPPIQNIPKPRRDEDSNYSRPDQGEAPARGSTEQEREREDEGPKMTLKDRMALLQNQQLDPTGLGWKNKRPSKPKRNESERTLEDDQSCAAPEDSSHEHRSHDSTRPQTTEGEGLEAAPKQKRSVDISREDTVVEESSAGDADISSASGYTGEETITSRSRLPTTSHREDKSDDGDDEGANDDTPDNPDSDDEEIDPEVARKLAIRERIAKMSGGMGMHGMFGGMPMGGPPPPPPKKKKSSSTSMPPKEEDESLRRSSNDGDESENKGSSDYTQSPPVALPFALPRVQSPPAAEQKSDSEAEAPARYIGGTSKRVFEEQSPDEVVDVEDIKSQQSQQTTPPHPQGPRPMPGSPILEKRHSQISPPSIPQDRPAPPPVPNERSAPPPPPSEPPTTQIPPLAARTPSDGSVSDDELSVHAGKLTISTSELGELRAAPPPPPSGPPSIPSGRPGSSYDALSPTSPTSKRVSQGYSNTQSSPTASPVTPGGQNKRASYQRAAASMDESRPDVAGSQRQSDTEEEVTEYEADYDTDIGNKVAHRDALKGKEVGRRDGHDDDEGTPLPSPIIAPQAPPRNVPLPPPPAPPSNPPPSRKGRQSMDMPRAAPPPPPPAPPSQEPERYEGPGDYDPYSYESPRLASPPPPPTSGLPPPPPHQAPPSQRDQPRGSAGRQSMDSSRPVAGRRSMEQGRPVAEFVARDVDLGERSQWWMTPNTPPPIFQNRFKELYFEVEESTSTRRGGRTTVTKDVYVLFHDYSQTVVTARYDRDDSANTSLEQRHEPPPPNPRQDQLEEFQTKYGANIYNGAKSRENTVVGDGEPHTLIYELFKLVPNALSPAGHRSYGALVYANLANANTILNDEIRAGDILSFRNARFQGHKGGLHQKYSMDVGKPDHVAIVVDWDGTKKKVRAYDQGRESRKVKIDSFKIGDLRSGEVKVWRVVGRDWVGWGRGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJJ0</accession>
    <name>D5GJJ0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00009037001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430332">
        <property type="protein sequence ID" value="CAZ84683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840492.1">
        <property type="nucleotide sequence ID" value="XM_002840446.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJJ0"/>
    <dbReference type="STRING" id="656061.D5GJJ0"/>
    <dbReference type="GeneID" id="9182684"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009037001"/>
    <dbReference type="eggNOG" id="ENOG502RZ7A">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043194_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJJ0"/>
    <dbReference type="OMA" id="FTHIPER"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043124">
        <property type="term" value="P:negative regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038753">
        <property type="entry name" value="NFKBIL1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15263">
        <property type="entry name" value="I-KAPPA-B-LIKE PROTEIN IKBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15263:SF1">
        <property type="entry name" value="NF-KAPPA-B INHIBITOR-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="16"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="193"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="46"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="63"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="193"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ84683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="347" mass="40785" checksum="EF4EBA4662DF31E9" modified="2010-06-15" version="1">MRPFEVNNDRLGVLYGLTQDPAPLRTSAPPTSPLKSKSRSRASPPPEKEGRAPESKANDLPRPRKSKFKFKRKHQNDSHHHRSPSSHSHSHSSSRRKRSHHKPPDDDPAEYDDTYIPNLASANFATAEDAFRESLFDAMADDEGAAFWEGVYGQPINVYPRPDGRGELEKMTDEEYAEYVRSKMWEKTHEHIIEERNRREEARRAKKQRDEEEQKMWEEGERSRRREEKTRRRLKEKTRVEKQQKGYLEKWREILAGKMDGDIPWPVESGKLKDIEKVSLERFYMDIRGCLGEDGLLDILKVDRIRWHPDKAQQRWGKRGLSEEEQRGITAVFQTVDSLWVKYKGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJP2</accession>
    <name>D5GJP2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00009095001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430332">
        <property type="protein sequence ID" value="CAZ84735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840544.1">
        <property type="nucleotide sequence ID" value="XM_002840498.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJP2"/>
    <dbReference type="STRING" id="656061.D5GJP2"/>
    <dbReference type="GeneID" id="9186769"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009095001"/>
    <dbReference type="eggNOG" id="ENOG502QTVK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033074_1_1_1"/>
    <dbReference type="InParanoid" id="D5GJP2"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="162"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="378"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="19"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="378"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="401" mass="44525" checksum="19EE1CEA0037FC25" modified="2010-06-15" version="1">MSLSGPSHFQHRASDDSDSSMGTSSDSSASRSTVPSSLPSLEQHESKTASPAESEIFDYHPAHDPRESENTYCSTVEDPDDDYPYYDTHPYVHYHKAPPPQASPSTPSEFAELFPSTRCLSIRHDDSTLDGNMNLRVAHIKRKTIKPAPQRPRLQRSVSKALHSFRGRTEAEPVAKLNRQDSGYSSSGFDGEEEEELKSSSEVTQNNNICTLEFSNYAHVDLTRRGGKGSKKYDFEYWGKSYSWKRVAKQVGPHAGAEEISYQLANNSTGNVVAHIVPDPLSPTQAQAEEAKGGIVPPCSFWLRDSSQDPIILCLADVADVIMATGLTALVDDCIKRRFHKKKTVQLVLPMPMIKNPMKMNLEYVGPKRLIDEVFNRRPTLSRSQSTQSSRSPLPVRSATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJS6</accession>
    <name>D5GJS6_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">homocitrate synthase</fullName>
            <ecNumber evidence="5">2.3.3.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">GSTUM_00009173001</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>acetyl-CoA + 2-oxoglutarate + H2O = (2R)-homocitrate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12929"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:58884"/>
            <dbReference type="EC" id="2.3.3.14"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:12930"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-alpha-aminoadipate from 2-oxoglutarate: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the alpha-IPM synthase/homocitrate synthase family. Homocitrate synthase LYS20/LYS21 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.14" evidence="5"/>
    <dbReference type="EMBL" id="FN430333">
        <property type="protein sequence ID" value="CAZ84769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840578.1">
        <property type="nucleotide sequence ID" value="XM_002840532.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJS6"/>
    <dbReference type="FunCoup" id="D5GJS6">
        <property type="interactions" value="622"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJS6"/>
    <dbReference type="GeneID" id="9186804"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009173001"/>
    <dbReference type="eggNOG" id="KOG2367">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022158_2_2_1"/>
    <dbReference type="InParanoid" id="D5GJS6"/>
    <dbReference type="OMA" id="NTMRMLV"/>
    <dbReference type="UniPathway" id="UPA00033">
        <property type="reaction ID" value="UER00028"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004410">
        <property type="term" value="F:homocitrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd07948">
        <property type="entry name" value="DRE_TIM_HCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.260:FF:000002">
        <property type="entry name" value="Homocitrate synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000032">
        <property type="entry name" value="Homocitrate synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02222">
        <property type="entry name" value="Homocitr_synth_fung_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050073">
        <property type="entry name" value="2-IPM_HCS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002034">
        <property type="entry name" value="AIPM/Hcit_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048253">
        <property type="entry name" value="DRE_TIM_HCS_fun_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011872">
        <property type="entry name" value="Homocitrate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054691">
        <property type="entry name" value="LeuA/HCS_post-cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02146">
        <property type="entry name" value="LysS_fung_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10277:SF48">
        <property type="entry name" value="HOMOCITRATE SYNTHASE, CYTOSOLIC ISOZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10277">
        <property type="entry name" value="HOMOCITRATE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22617">
        <property type="entry name" value="HCS_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00815">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00816">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0464">Manganese</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="15">
        <location>
            <begin position="49"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAZ84769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="466" mass="51154" checksum="AC66DC73DC516DCE" modified="2010-06-15" version="1">MPPAASGNGSPSSNEKKDFQPISGRHGPHLNSRNPYQPVPDFLSNVNSFKIIESTLREGEQFANAFFDTAKKIEIAKALDDFGVDYIELTSPAASEQSRLDCEAICKLGLKAKILTHIRCHMDDARVAVQTGVDGVDVVIGTSSFLREHSHGKDMDYIHKTAIEVIEFVKSHNIEIRFSSEDSFRSELVDLLSIYRTVDQIGVNRVGIADTVGCASPRQVYDLVRTLRGVVSCDIETHFHNDTGCAVANAYCALEAGATHIDTSVLGIGERNGITPLGGLMARMIVADREYVTSKYKLHKLRDLENLVAEAVEVNIPFNNYITGFCAFTHKAGIHAKAILANPSTYEIINPADFGITRYVHFASRLTGWNAIKSRVEQLDLDMTDAQCKECTAKIKQLADVRPLNVDDVDGIIRQYHFNIKTGNNQPLLKGIDHPAPGIQEAAADTGEPAAKKLRTELDQDAAKCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJW1</accession>
    <name>D5GJW1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00009231001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430334">
        <property type="protein sequence ID" value="CAZ84804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840613.1">
        <property type="nucleotide sequence ID" value="XM_002840567.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJW1"/>
    <dbReference type="FunCoup" id="D5GJW1">
        <property type="interactions" value="534"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJW1"/>
    <dbReference type="GeneID" id="9186840"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009231001"/>
    <dbReference type="eggNOG" id="KOG2407">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021459_2_1_1"/>
    <dbReference type="InParanoid" id="D5GJW1"/>
    <dbReference type="OMA" id="NHGHYIG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042765">
        <property type="term" value="C:GPI-anchor transamidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016255">
        <property type="term" value="P:attachment of GPI anchor to protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007245">
        <property type="entry name" value="PIG-T"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12959:SF11">
        <property type="entry name" value="GPI TRANSAMIDASE COMPONENT PIG-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12959">
        <property type="entry name" value="GPI TRANSAMIDASE COMPONENT PIG-T-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04113">
        <property type="entry name" value="Gpi16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072403" evidence="2">
        <location>
            <begin position="23"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="494"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ84804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="556" mass="61486" checksum="A1EDF447F24C3F51" modified="2010-06-15" version="1">MRLLSWPSVTQLLLAPLVIASASEYNDYSENLLLKSLPGNSLLASFRFVSNGTFDSHHSVFPRSLAQILQQSQTKELHLKFTLGRWDAEQWGRQVWDGRTAGGTGVELWAWVDAADDAEADARWTTLNNALSGLFCASLNFIDSTRTIRPVMSFQPAGHHSPEGLERLHLLHGTLPREPVCTENLTPFLKLLPCKGKAGISSLLDGHRLFDAQWQSMSIDVKPVCEEDGKCQLQMEQTKPYAGGDSCFPLSGVTDLPWSLSEMFGRPIRGSCPLAVVPTEEATHVCVNIAEQRSIFVSQGSEERKKSPELRCFILHENIDFDIVLPELSPSTNLLPTTPPVYASRSLTGHGQERGGIQAVLNNPSSNSTVEFVYLESLPWFMKPYLHTLSATISGKDDRNIIQDIYYRPALDRKRGTQLEVRLRVPPGETVLLNYEFDKAVLRYTEYPPDANRGFDIAPAVISILDPASQKSTFSIRTTSLLLSLPTPDFSMPYNVIILTSTVMALAFGSIFNILVRRFVGADEVIGGGLVSKIRGHIMAFAAKRGRAGGDKPKTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKV7</accession>
    <name>D5GKV7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00009787001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430341">
        <property type="protein sequence ID" value="CAZ85150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840959.1">
        <property type="nucleotide sequence ID" value="XM_002840913.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKV7"/>
    <dbReference type="GeneID" id="9183386"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009787001"/>
    <dbReference type="HOGENOM" id="CLU_2607761_0_0_1"/>
    <dbReference type="InParanoid" id="D5GKV7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="35"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="79" mass="9113" checksum="588EA209C43BD2AE" modified="2010-06-15" version="1">MISRATYICHRCVFRRSLQSALQLRVQSTGPKFLADGLLRRAQQLSNEHSELEKQLADNYDKKLATRAGRISKFHSRIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GL50</accession>
    <name>D5GL50_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00010002001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430346">
        <property type="protein sequence ID" value="CAZ85243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841052.1">
        <property type="nucleotide sequence ID" value="XM_002841006.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GL50"/>
    <dbReference type="GeneID" id="9185837"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010002001"/>
    <dbReference type="HOGENOM" id="CLU_153394_0_0_1"/>
    <dbReference type="InParanoid" id="D5GL50"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="139" mass="15210" checksum="B7894BC397E23ACE" modified="2010-06-15" version="1">MTYVSAQLFLILFVLPLMQTPFLPSLSNIVTPLDPSTPRPPPSFFLFSLSFSSLLTSSLLPAPTTPLSIVITVFASFFSLYFPSRQSFHSCHGGKILEICFQIISGNQLPTRLNLVGLPYTAGSRGLSRCARLPLLTSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>D5GL94</accession>
    <name>D5GL94_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00010079001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430347">
        <property type="protein sequence ID" value="CAZ85287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841096.1">
        <property type="nucleotide sequence ID" value="XM_002841050.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GL94"/>
    <dbReference type="GeneID" id="9182081"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010079001"/>
    <dbReference type="HOGENOM" id="CLU_2308125_0_0_1"/>
    <dbReference type="InParanoid" id="D5GL94"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ85287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="100" mass="10909" checksum="2B3697A43D37CC87" modified="2010-06-15" version="1">MDDWLIACLFPSPPPPFPGFAFSNRWNGLPVVRLRKCNHAFCAECVRGKMTTREGIVCAFCGRVAELLVGISAPMSMPGEDDVVLTNVVVLQESKVVEIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLC2</accession>
    <name>D5GLC2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00010127001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430348">
        <property type="protein sequence ID" value="CAZ85315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLC2"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010127001"/>
    <dbReference type="HOGENOM" id="CLU_2706596_0_0_1"/>
    <dbReference type="InParanoid" id="D5GLC2"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ85315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="73" mass="8413" checksum="6240CD6C1FB30362" modified="2010-06-15" version="1">MIYRHSSYACPTCREEGKSMLLKTTRENGIKKVWSTVEPSWFGDHTSKSTIYSMSTRVQEKHGKDVGRAQNLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLF3</accession>
    <name>D5GLF3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00010170001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430348">
        <property type="protein sequence ID" value="CAZ85346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841155.1">
        <property type="nucleotide sequence ID" value="XM_002841109.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLF3"/>
    <dbReference type="STRING" id="656061.D5GLF3"/>
    <dbReference type="GeneID" id="9186090"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010170001"/>
    <dbReference type="eggNOG" id="KOG1968">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003721_0_0_1"/>
    <dbReference type="InParanoid" id="D5GLF3"/>
    <dbReference type="OMA" id="RNHLVQQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23389:SF21">
        <property type="entry name" value="ATPASE FAMILY AAA DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23389">
        <property type="entry name" value="CHROMOSOME TRANSMISSION FIDELITY FACTOR 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="2">
        <location>
            <begin position="371"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="185"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="276"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="839"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="321"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ85346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="953" mass="104672" checksum="CD61531664DA5A01" modified="2010-06-15" version="1">MKKLDNTPITNPPSGDGADRVKTISPGLPDQPQGSSCFFGNSQTHLPSVRLGVRGPVSYLNSVNSRSLRNSGAVGPPWPSKYNCHVRGPSEEGSIVHRLEGELVLKERKLKDAVVAISPEEDILRQFTSHLDINAQREDMLSADYHERRFREIPCDIRLPKRVVTTGIKFQEAVRERVAARLPHPNNSALELPRSRENSVSSISSGGLETRPHQALLNLYDRLGRELSAFDKDECESQAWEAKYAPTSSSEVLQIGKEPRILKDWLVALKVENVGTGSNNQKQGTKGKKKHSLGAAAGKAATRRKKKRKKDDDELAGFIIDSEEEQNEMDEITDPEDTDYSTIPGVKKSTIRAGDKGTDSPSGILREPDRLVNAVVISGPNGCGKTATVYAVAKEVGFTVFEVSPGAKRGGKDILDLVGEMTRNHLVHQTKAGASASATSFFKSKIGNKAQAEEDLQLNKSQQQSLLLLEEVDLLFDEDKQFWSTVLSLIAQSKRPVVMTCNDEGLLPIDLLSLHAVLRLTPPPMDLIVDHLLLICANEGHMLRREAVMTLVKATGRDLRASLMELEFWCRMGVGDRKGGLDWMLVRWPSGSDRDENGNVLRVISRNTYRKGMDWILRGASDEAKWNDVWEKWGVDVGSGEDKELKDFSTGFGEDLQSLEIWDDYAEAMSSVDMCAGSGMDSTEAAFDTTLPLIGAKVRLDDVMGYQTLQTDPVATPWSTETNISICVRSLARVHLNDKATALSISPLNAIRDEEIISTIADRGSIRDELSPLSDIYIREAFQSLGSTSTIFSPAPSISHSVVTGSRVALMIDAAPYVRGITRHDLAREEERKRISGLLSQGGRNGKRRLTRAARVAEGGGDRRSRERWFGQLNARLVEGSGGAGWGDTTITPSQGMSDSGNGVSFMAFTQARVLTRYVPYRYQVFQIGTVPGWLAADWTGGGQQGGCNEQCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLJ7</accession>
    <name>D5GLJ7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00010239001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the isochorismatase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430349">
        <property type="protein sequence ID" value="CAZ85390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841199.1">
        <property type="nucleotide sequence ID" value="XM_002841153.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLJ7"/>
    <dbReference type="STRING" id="656061.D5GLJ7"/>
    <dbReference type="GeneID" id="9186134"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010239001"/>
    <dbReference type="eggNOG" id="KOG4044">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066901_0_0_1"/>
    <dbReference type="InParanoid" id="D5GLJ7"/>
    <dbReference type="OMA" id="HVCVFQT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.850">
        <property type="entry name" value="Isochorismatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000868">
        <property type="entry name" value="Isochorismatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036380">
        <property type="entry name" value="Isochorismatase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050993">
        <property type="entry name" value="Isochorismatase_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14119">
        <property type="entry name" value="HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14119:SF3">
        <property type="entry name" value="ISOCHORISMATASE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00857">
        <property type="entry name" value="Isochorismatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52499">
        <property type="entry name" value="Isochorismatase-like hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isochorismatase-like" evidence="2">
        <location>
            <begin position="11"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ85390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="199" mass="22009" checksum="EF04910EC4231D32" modified="2010-06-15" version="1">MVLRGRINNPVLFVCDLQEKFRTAIYGFDKCVLTAQKLLKVGQILQIPTYATTQLASKLGPLVPELHPLLVNAKVLDKSLFSMMTPELRARLDEDGDDGEKKSVALVGIESHICVLQTCLDLLAAGHEVYVVADGVSSCNRQEVPVALERMRREGAVVTTSESWMYEVMGDAKISEFRQMVGLVRESKESTTLALQSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLL6</accession>
    <name>D5GLL6_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Hsp90 chaperone protein kinase-targeting subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00010261001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDC37 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430349">
        <property type="protein sequence ID" value="CAZ85409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841218.1">
        <property type="nucleotide sequence ID" value="XM_002841172.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLL6"/>
    <dbReference type="FunCoup" id="D5GLL6">
        <property type="interactions" value="411"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GLL6"/>
    <dbReference type="GeneID" id="9182712"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010261001"/>
    <dbReference type="eggNOG" id="KOG2260">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033261_0_0_1"/>
    <dbReference type="InParanoid" id="D5GLL6"/>
    <dbReference type="OMA" id="NYSKWDQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.610:FF:000002">
        <property type="entry name" value="Hsp90 co-chaperone Cdc37, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.610">
        <property type="entry name" value="Cdc37, Hsp90 binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004918">
        <property type="entry name" value="Cdc37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013873">
        <property type="entry name" value="Cdc37_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013874">
        <property type="entry name" value="Cdc37_Hsp90-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038189">
        <property type="entry name" value="Cdc37_Hsp90-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013855">
        <property type="entry name" value="Cdc37_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12800">
        <property type="entry name" value="CDC37-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12800:SF4">
        <property type="entry name" value="HSP90 CO-CHAPERONE CDC37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08564">
        <property type="entry name" value="CDC37_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08565">
        <property type="entry name" value="CDC37_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03234">
        <property type="entry name" value="CDC37_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01069">
        <property type="entry name" value="CDC37_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01070">
        <property type="entry name" value="CDC37_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01071">
        <property type="entry name" value="CDC37_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101391">
        <property type="entry name" value="Hsp90 co-chaperone CDC37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cdc37 N-terminal" evidence="9">
        <location>
            <begin position="2"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="Cdc37 Hsp90 binding" evidence="8">
        <location>
            <begin position="183"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="Cdc37 C-terminal" evidence="7">
        <location>
            <begin position="382"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="174"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="470"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="187"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="227"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="485"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ85409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="515" mass="57101" checksum="1CAB6381D7077268" modified="2010-06-15" version="1">MVLDYSKWDNIELSDDSDIEVHPNVDKRSFINAKRRQIHEQRAMRKQQITNLRTERSMNNALLSRVSQLISTLDAGSKGSKSPEEIVFQSLMDISSADNNPQEFTPPEGAPSYAQMMASLVDSIKKEIDEENPDDRWTSFLNKLKEHKVKLTGVYEDSGKRLADLEKEDNAKITSDSIHDGFSSGHVTKESPLPKKEPAKKTQKVQAVEQLNPGVKAKGPLATDPPSDADAEEGGDEADEDEDHIEPTALGREFSHIKMGDYRACMEFISRNSAVVAEKETDGLLIEAFNSQIAGKEDYAKQCVHQALLLQYCRQLGRDGIALFFRRITTPNHQAQKVFHEDVTTTYARIRERAAAIATESSNENAEQIQLHAVDPNTQISIAVPSVDSEDPDIQKSRTIFESFPPGLQRALGSGSLDEVNKVLGKMSVEEAEEVVGQLGEGGMLSLEPQIIDATTQEGQQVLRKIEEEERAAKANASSSPDITETDRKGKGISKEEKGKEEEALKEEDLANEID</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLP2</accession>
    <name>D5GLP2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00010295001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5 6">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the group II decarboxylase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430350">
        <property type="protein sequence ID" value="CAZ85435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841244.1">
        <property type="nucleotide sequence ID" value="XM_002841198.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLP2"/>
    <dbReference type="STRING" id="656061.D5GLP2"/>
    <dbReference type="GeneID" id="9182738"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010295001"/>
    <dbReference type="eggNOG" id="KOG0628">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011856_6_2_1"/>
    <dbReference type="InParanoid" id="D5GLP2"/>
    <dbReference type="OMA" id="SWATDAH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010977">
        <property type="entry name" value="Aromatic_deC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11999:SF165">
        <property type="entry name" value="DECARBOXYLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G04980)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11999">
        <property type="entry name" value="GROUP II PYRIDOXAL-5-PHOSPHATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 5 6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="5">
        <location>
            <position position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ85435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="503" mass="54349" checksum="546DB78D07AD8E2A" modified="2010-06-15" version="1">MHPQSVFKTGSPPPSNSTNPIHPTAAHFLTQLLSTLSALPHPVPDPQTITAARTILTHHLPHTGLGLEHTAQHLLSLLPSFARTTAHYYAFVTGSVTPAAHLADLLVSTVDANVQVHLPQDSLVTEVEDVALRLLCQLFRLDEREWAGRVITTGATAGNVLGLACGREEVLNRRLRARGVDEGRGGVGELGLLEACIRAGATGVKVFSAMAHSSLLKAAAVVGIGRGNVVDIGRVEKPWEIDLARLKEELERAEAEGVVSIVVVSFGEVNTGRFDGGMEEIRKLADEYGAWLHTDAAFGIFARVLGSEESGDGGFARGLELADSIVGDGHKILNVPYDCGFFFTRDSRVLLATFVNTAPYLSPSSSPDGIQSPLNLGLENSRRFRALPVYATLCAYGVSGYKDLLSRLVAHSRNIATFIHGHGSYQLLNGPIGDIFVIVLFSAKEDGLNHRLKKAINDTRKMYVSGTMWSNRPAIRIAVANWQVESDELEVVKEVLQELARQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLV2</accession>
    <name>D5GLV2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Mitochondrial import inner membrane translocase subunit TIM14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Mitochondrial import inner membrane translocase subunit tim14</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11 13">Presequence translocated-associated motor subunit PAM18</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00010431001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the TIM14 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430351">
        <property type="protein sequence ID" value="CAZ85519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841328.1">
        <property type="nucleotide sequence ID" value="XM_002841282.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLV2"/>
    <dbReference type="STRING" id="656061.D5GLV2"/>
    <dbReference type="GeneID" id="9186503"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010431001"/>
    <dbReference type="eggNOG" id="KOG0723">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017633_13_3_1"/>
    <dbReference type="InParanoid" id="D5GLV2"/>
    <dbReference type="OMA" id="MXAFSGG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001405">
        <property type="term" value="C:PAM complex, Tim23 associated import motor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000001">
        <property type="entry name" value="Import inner membrane translocase subunit tim14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12763">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12763:SF28">
        <property type="entry name" value="GEO10507P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00067223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00075483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ85519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="99" mass="11197" checksum="62DC1597401DD396" modified="2010-06-15" version="1">MAKLIPPQARIGLQAFKRYKALGSGAGAFGRNFYRGGFEARMNRREAALILQLSERSATKKNIRKRHREMMLLNHPDRGGSPYLAGKINEAKEFLEKHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLZ8</accession>
    <name>D5GLZ8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00010337001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430351">
        <property type="protein sequence ID" value="CAZ85460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841269.1">
        <property type="nucleotide sequence ID" value="XM_002841223.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLZ8"/>
    <dbReference type="GeneID" id="9182763"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010337001"/>
    <dbReference type="HOGENOM" id="CLU_2591521_0_0_1"/>
    <dbReference type="InParanoid" id="D5GLZ8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="80" mass="8765" checksum="44DBE0EB95DC86E9" modified="2010-06-15" version="1">MKRQTLSCYHVSLLSRNLSKDNLQHQATPAPTSLLPTSQISSGPKTEHASPRANQEIKPLLLNPLLPFYLSPLKTLGTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>D5GM18</accession>
    <name>D5GM18_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="13">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00010485001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NDC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430351">
        <property type="protein sequence ID" value="CAZ85561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841370.1">
        <property type="nucleotide sequence ID" value="XM_002841324.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GM18"/>
    <dbReference type="STRING" id="656061.D5GM18"/>
    <dbReference type="GeneID" id="9187341"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010485001"/>
    <dbReference type="HOGENOM" id="CLU_963750_0_0_1"/>
    <dbReference type="InParanoid" id="D5GM18"/>
    <dbReference type="OMA" id="FWELAYL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070762">
        <property type="term" value="C:nuclear pore transmembrane ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006999">
        <property type="term" value="P:nuclear pore organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070631">
        <property type="term" value="P:spindle pole body localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019049">
        <property type="entry name" value="Nucleoporin_prot_Ndc1/Nup"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13269">
        <property type="entry name" value="NUCLEOPORIN NDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13269:SF6">
        <property type="entry name" value="NUCLEOPORIN NDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09531">
        <property type="entry name" value="Ndc1_Nup"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="10" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ85561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="289" mass="31409" checksum="69929BA5B66DCFD6" modified="2010-06-15" version="1">MLAFWELAYLAQNSAARRKAIYTDVNRKPANIFEQILSQSLSVLGEVDLKLAEVRNPPAPTALILATPSSTAASAIPSPPPIPISQINPLLISPVSSTHKFLEGVQSKDGSTPTLQHVIEKLPCQPPSIAEVEASATKSFKSKMEPFLSSPWGSMFRQSIHATTSQLIPNVGLQVNAVQAISRLVTSSLAEDEYGIVQSHAPTLLASFISMMDTLDKYIASPPISWTDVESIQKQEKGELHLAEPHLLLSTLKEGFKDIATAYAPYIDDMGLASDTRKIILETRERGLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5GM66</accession>
    <name>D5GM66_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00010545001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430352">
        <property type="protein sequence ID" value="CAZ85609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841418.1">
        <property type="nucleotide sequence ID" value="XM_002841372.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GM66"/>
    <dbReference type="STRING" id="656061.D5GM66"/>
    <dbReference type="GeneID" id="9187393"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010545001"/>
    <dbReference type="eggNOG" id="ENOG502SB54">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065618_1_0_1"/>
    <dbReference type="InParanoid" id="D5GM66"/>
    <dbReference type="OMA" id="PAIITWI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018466">
        <property type="entry name" value="Kre9/Knh1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052982">
        <property type="entry name" value="SRP1/TIP1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40633:SF1">
        <property type="entry name" value="GPI ANCHORED SERINE-THREONINE RICH PROTEIN (AFU_ORTHOLOGUE AFUA_1G03630)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40633">
        <property type="entry name" value="MATRIX PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_8G05410)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10342">
        <property type="entry name" value="Kre9_KNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072144" evidence="4">
        <location>
            <begin position="21"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="214"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="Yeast cell wall synthesis Kre9/Knh1-like N-terminal" evidence="5">
        <location>
            <begin position="48"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="130"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="132"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF10342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ85609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="232" mass="22800" checksum="3128622AD2F6CE4D" modified="2010-06-15" version="1">MRFFTVALGTVVAFAALISAQTTTGTGPPPPAVTDSTENQINVPPGGTIASGSGITIRWTPSTPGPVTLILRRGDGNNLATVTNIASEIPNSGSYDWTPSESLPVGDDYSIQIISGSASNYSPKFGIGPPVSTSSSSTSTTTSTGSSSTSSSSSSSSSTITSSSSSSSATTSATSSGANTTISSTSRTSSRSIPTSTSSGATGGATSTNGAAQVVSPLALVFFIAAGIAYLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>D5GM88</accession>
    <name>D5GM88_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00010576001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the actin family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430353">
        <property type="protein sequence ID" value="CAZ85625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841434.1">
        <property type="nucleotide sequence ID" value="XM_002841388.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GM88"/>
    <dbReference type="FunCoup" id="D5GM88">
        <property type="interactions" value="802"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GM88"/>
    <dbReference type="GeneID" id="9187409"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010576001"/>
    <dbReference type="eggNOG" id="KOG0797">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006974_0_1_1"/>
    <dbReference type="InParanoid" id="D5GM88"/>
    <dbReference type="OMA" id="AYKCMWA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034080">
        <property type="term" value="P:CENP-A containing chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd10206">
        <property type="entry name" value="ASKHA_NBD_Arp8-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000201">
        <property type="entry name" value="Actin-related protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000232">
        <property type="entry name" value="Actin-related protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="139"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="549"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="148"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="158"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="562"/>
            <end position="581"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ85625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="724" mass="81757" checksum="0D040735358F6292" modified="2010-06-15" version="1">MAPPKKSGRALLREEGLERTDNNLRTHSWPVIPMINQKNYYTEYLKREEQIMVFRQQQEDLLRAQAALLEKEKQVSMVADPMAMDIDRGAEEGEETAEQEEALGSKVIVIHTGSQNMRIGLASDALPRSFPNCIARKADKAEYEEEERCPKRMKVAEENEDSDDEGPQFGEEFDSQFNAMSNELKVRMRHNKRRILPNSRDLVLSYNRRTPPETIKEHNDPYRVEWTDTGRNKAPDHFVGNEALRVPDNSDPRYRLFWPIQYGTFNERDYHSKRRVLEDISVILEEGIKRELNIEKKDLKLYSAVLVVPDLYDKNYVTEMLNLLLKEFWFAQVCIIQESLAATFGAGFSQACIVDIGAQKTSVCCVEEGMCLAESRLNLKYGGRDVTIALMKMMLSYSFPYRDINLRRRYDFLLAEELKAKYATLNDSDISVQAHDFHLRAPEQDTKKYHFKTYDEVMLAPLTHFKPTILSHEEKLTGRRSLWERSYDLYDGQPNDPLSTAQHSLYQSLMPRPSSTGNDSTVNMGAITPARPTLAGFGGFGAHLTAADTTPRSSIAGSPAPEGTPAPPGTTTPLVPGPGGIPHPGSVGAPQPTGPTPMEQAEDRDRVVPIMPLDAAIVESITHAAKGEEKKIKDFLASIMVVGGGSLVSGFNHQLEERIRALKPSKDYQIIIGVPPRELDPMVIVWKGASVFGKLKISNDSWIRQNEYDILGSRLLTYKCLWTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMH1</accession>
    <name>D5GMH1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00010713001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430355">
        <property type="protein sequence ID" value="CAZ85714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841523.1">
        <property type="nucleotide sequence ID" value="XM_002841477.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMH1"/>
    <dbReference type="GeneID" id="9182140"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010713001"/>
    <dbReference type="HOGENOM" id="CLU_3070388_0_0_1"/>
    <dbReference type="InParanoid" id="D5GMH1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ85714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="53" mass="6261" checksum="ECBBA81F3F2B507A" modified="2010-06-15" version="1">MHRLYYKDGSTRYSISTSTYIVLYNTTVLIYGKTCNNIRGMSATLWLQYKNQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMN7</accession>
    <name>D5GMN7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00010833001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430357">
        <property type="protein sequence ID" value="CAZ85780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841589.1">
        <property type="nucleotide sequence ID" value="XM_002841543.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMN7"/>
    <dbReference type="GeneID" id="9185116"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010833001"/>
    <dbReference type="HOGENOM" id="CLU_2905807_0_0_1"/>
    <dbReference type="InParanoid" id="D5GMN7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="62" mass="7472" checksum="EC29CE9874160858" modified="2010-06-15" version="1">MHYPTALRLPKPPRLHNSVLEYGRRSKENEGRGEKKKKKEKSRFARQNPAHHALWVRLYSYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMR4</accession>
    <name>D5GMR4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitochondrial division protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00010895001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Involved in mitochondrial fission. Acts as an adapter protein required to form mitochondrial fission complexes. Formation of these complexes is required to promote constriction and fission of the mitochondrial compartment at a late step in mitochondrial division.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the WD repeat MDV1/CAF4 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430359">
        <property type="protein sequence ID" value="CAZ85807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841616.1">
        <property type="nucleotide sequence ID" value="XM_002841570.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMR4"/>
    <dbReference type="FunCoup" id="D5GMR4">
        <property type="interactions" value="38"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GMR4"/>
    <dbReference type="GeneID" id="9185143"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010895001"/>
    <dbReference type="eggNOG" id="KOG4155">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012350_1_1_1"/>
    <dbReference type="InParanoid" id="D5GMR4"/>
    <dbReference type="OMA" id="ERLRYMD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990234">
        <property type="term" value="C:transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22881">
        <property type="entry name" value="Mdv1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000881">
        <property type="entry name" value="Mitochondrial division protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847:SF637">
        <property type="entry name" value="WD REPEAT DOMAIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 11" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="311"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="353"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="409"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="493"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="516"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="140"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="249"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="146"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="252"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00043913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ85807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="628" mass="69370" checksum="7BD63DDBE07EFA39" modified="2010-06-15" version="1">MASNTGHGSSPSTLDRPRTEEGDATIISNALTSVAGQNISAFGRTVSATAGTLFTDPAAPPQHYAAAMSDISKQLSHRPSLQRRVFSFNKRSPTELVRSKLSTAEIQYRALTYLPDELLRNIPDNENSYSLFQGFQASMPEEHQRVGKNHPRKGSKNKLLEASNGGEPDTPLGNLKREKSTMARQLEMLSIRKNMASSEIREIDMKISNLNQMRNIVLDRLAKLEQDEAEAEHEILVLENKIEDLQEELDEENHHSTDPESDNRSEDSTFMSESIYGKLNTPKSRKRKSSRRISMPVLHEHLEPGQNIKEIQTHADSITSLDFDVPFGTLVTAAFDDTIRVWDMNTGRCTGLLEGHQASVRCLQVEDNMVATGSVDASIRLWDLSRAETLPLVQEFALPSLSECYVFSLDSHVDEVTALYFQGDTLVSGSADKTLRQWDLVKGRCVQTLDVLWAAAQSNMDEGKWRNGARSAGGVSGHEGDFVGALQCFDAALACGTADGMVRLWDLRSGQVHRSLVGHTGPVTCLQFDDVHLVTGSLDRSIRIWDLRTGSIFDAFGFDNPITSMQFDSRRIVSAAGETVAKIYDKADGRHWDCGAVGVDAAIVDRVRVNDGYLVQGRRNGTVGIWAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMV1</accession>
    <name>D5GMV1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00010948001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430360">
        <property type="protein sequence ID" value="CAZ85844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841653.1">
        <property type="nucleotide sequence ID" value="XM_002841607.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMV1"/>
    <dbReference type="GeneID" id="9183412"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010948001"/>
    <dbReference type="HOGENOM" id="CLU_3034077_0_0_1"/>
    <dbReference type="InParanoid" id="D5GMV1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="55" mass="6214" checksum="AEC4686CBD7B6E5D" modified="2010-06-15" version="1">MAVFSGEPLRCANPFANHFIPSYIILPRITPPQKKKKKNETPIPISESTHPMPSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMX5</accession>
    <name>D5GMX5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="11">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00010982001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oligopeptide OPT transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430360">
        <property type="protein sequence ID" value="CAZ85868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841677.1">
        <property type="nucleotide sequence ID" value="XM_002841631.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMX5"/>
    <dbReference type="FunCoup" id="D5GMX5">
        <property type="interactions" value="91"/>
    </dbReference>
    <dbReference type="GeneID" id="9183436"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010982001"/>
    <dbReference type="eggNOG" id="KOG2262">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004965_4_2_1"/>
    <dbReference type="InParanoid" id="D5GMX5"/>
    <dbReference type="OMA" id="MTFDWAQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035673">
        <property type="term" value="F:oligopeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004648">
        <property type="entry name" value="Oligpept_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00727">
        <property type="entry name" value="ISP4_OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00728">
        <property type="entry name" value="OPT_sfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22601">
        <property type="entry name" value="ISP4 LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
        <property type="entry name" value="OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0571">Peptide transport</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="284"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="324"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="361"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="394"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="469"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="531"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="556"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="638"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="710"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="743"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="766"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="795"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="19"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ85868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="858" mass="95489" checksum="A166528D12CD5F5B" modified="2010-06-15" version="1">MGNGLGGSSYTGSAPSSTTEEEEAEESIPEAEDDSIVEEVGVAEETEGNGLLSLEGRGGGSEGTRNNRRRQTDTVGLGLRERESKDTGKWVMDGSADTNGEHCYGILGDSDEEEIGDDPIDNSPYPEVRASVSAKDDPDILINTPRMWALAMFFSLVGSATNLFFSLRYPSVSITPVIALLLAHPLGKLWDHVFPEADATEVVKSEDGWWGRLKTRLGQGRWNQKEHSCVYISSNVSFGFAFATDVIVEQTHFYHQNPGITYQILLTLSTQILGYTFAGLTRQWLVYPGAMIWPGTLMSTAMFTTLHGEENKVANGWRISRFKFFLVVFAGGFFWYFVPGLLIPALSYFNVVTWFAPDNVPIANLFGVASGLGMFPLTFDWAQISYVGSPLMTPFWAAANVVAGLVAVMWVAAPILYYGNAFYSSYMPILSSAVFDNTGSPYDVSKILTKDFLFDELAYKNYSRVFMPITYILSYGLQFAALTALVTHTACWHGKDIWRQWGRARRGFSSGRREDVHMRLMRRYREAPTSWYLATFVVMIAVGIFVVEYYPVHLPWYGLLLALCVCGTFFVPIGIVMAITNQQSSIFLICQLICGTLFPGRPVANMVFVTYGYITATQGLKFAADLKLGHYMKIPPRLLFSVQMAATCVSSFTQIGVLNWMFAYIPGLCTPQAMNGFTCPIARVHFNGSILWGVVGPGRFFGRGELYRSLMWAFVVGAFTPIILFAVYKYAGRGGNSRRTKVAWLRKVSLPVVFGSLSWIPPATGLNFSTWVIVCFAFNYYIFRRAPGWWGKYTMTLSAALDASLAVGLVVVFFGFVYPGWMEGFSWWGTEVYKRGCDWKACAYLEVPEGGVFGPSRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNA3</accession>
    <name>D5GNA3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00011193001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430362">
        <property type="protein sequence ID" value="CAZ85996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841805.1">
        <property type="nucleotide sequence ID" value="XM_002841759.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNA3"/>
    <dbReference type="GeneID" id="9182195"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011193001"/>
    <dbReference type="eggNOG" id="KOG1558">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023057_1_1_1"/>
    <dbReference type="InParanoid" id="D5GNA3"/>
    <dbReference type="OMA" id="GTLCEFY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013536">
        <property type="entry name" value="WLM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053000">
        <property type="entry name" value="WSS1-like_metalloprotease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036443">
        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46622">
        <property type="entry name" value="DNA-DEPENDENT METALLOPROTEASE WSS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46622:SF1">
        <property type="entry name" value="DNA-DEPENDENT METALLOPROTEASE WSS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08325">
        <property type="entry name" value="WLM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90209">
        <property type="entry name" value="Ran binding protein zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51397">
        <property type="entry name" value="WLM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="WLM" evidence="7">
        <location>
            <begin position="1"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="6">
        <location>
            <begin position="346"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="198"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="304"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="372"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="210"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="328"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ85996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="462" mass="50765" checksum="9E9D7479C3D0938D" modified="2010-06-15" version="1">MTQDQIIGTYVHIPGKPREQHALYMLRWIASLVKPIMNKGGYKVGCLAEFYPVQKSLLGLNVNNGEKVCIRLRQPYDDSVFLDIEECVYTMLHEITHNLHGPHNDTFYAHLKTLEESYSTLRRGGYDGEGFYSEGKRLGAGIPKNPLMSEARRRALAMAEKRRDIYSGSGQMLGGGKDPLPSGLGIREKIAAATERRIRDSQTCGAGSGARDRGAMEAEAERTIRAGTVTVGEEEEEEETKQEVVVLDEDPENEAAIMNAAIELIQEAEREEAEQKLLWEEQDGIVWIVDDEDGPIQVELPPAAATTAATTPSPPHPRIPAPIVITSSPPPPPPAEPQDLPPSYEQATTWACDLCTLINPSILPRCEACSFERPDSTDSATSPPPTPLSPIYQNPPLTQAVIEGKERERERQKVRFLDMPLMIPEKRGEAATPAGWMCVRCGTGNGEGWWGCGGCGVVRGEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNA4</accession>
    <name>D5GNA4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Golgi apparatus membrane protein TVP38</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Golgi apparatus membrane protein tvp38</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00011194001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Golgi membrane protein involved in vesicular trafficking and spindle migration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TVP38/TMEM64 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430362">
        <property type="protein sequence ID" value="CAZ85997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841806.1">
        <property type="nucleotide sequence ID" value="XM_002841760.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNA4"/>
    <dbReference type="FunCoup" id="D5GNA4">
        <property type="interactions" value="101"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GNA4"/>
    <dbReference type="GeneID" id="9182196"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011194001"/>
    <dbReference type="eggNOG" id="KOG3140">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041954_0_0_1"/>
    <dbReference type="InParanoid" id="D5GNA4"/>
    <dbReference type="OMA" id="KWQALET"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000022">
        <property type="term" value="P:mitotic spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051076">
        <property type="entry name" value="Golgi_membrane_TVP38/TMEM64"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032816">
        <property type="entry name" value="VTT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47549:SF1">
        <property type="entry name" value="GOLGI APPARATUS MEMBRANE PROTEIN TVP38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47549">
        <property type="entry name" value="GOLGI APPARATUS MEMBRANE PROTEIN TVP38-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09335">
        <property type="entry name" value="VTT_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="116"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="230"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="271"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="VTT" evidence="12">
        <location>
            <begin position="137"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="338"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="31"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="343"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF09335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ85997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="392" mass="42643" checksum="93CAF3D0B51CD972" modified="2010-06-15" version="1">MSEIPRHSGALYTPPVAHPPPPSGLRWNQQRRSSSLSSGPSRRSSGKGRRSALIARASRLQRWAHKRFNKMTLTQKILGSLVLLVMGTTAILGLIFHAKILHAMLPLAAKLRSWKVGWLLIFALCFASAFPPMIGYSTSVTLAGFVYGFPNGWFIIASATVIGATCAFIVCRYYIQDFAKRMVATDKRFAALSLTLKHDGIKLLCMIRLCPLPYSISNGAMSTFPTVSPLSFMVATAVATPKLLIHIFVGAKLAELAEEEGKKMDLKTKALNYGSIVGGVFLGIITGWLIYKRTMFRAKQLEADERARIRAPAGQGFTDVEDPDAEAAAGLVEDLIEGGYPDSVDDDESEEGDALLNSDDEDPGFGMEDSLELERNDTKGHSQAPPKGPIAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNF0</accession>
    <name>D5GNF0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Kynurenine 3-monooxygenase</fullName>
            <ecNumber evidence="11">1.14.13.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Biosynthesis of nicotinic acid protein 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Kynurenine 3-hydroxylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">BNA4</name>
        <name evidence="14" type="ORF">GSTUM_00011259001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn). Required for synthesis of quinolinic acid.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>L-kynurenine + NADPH + O2 + H(+) = 3-hydroxy-L-kynurenine + NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:20545"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57959"/>
            <dbReference type="ChEBI" id="CHEBI:58125"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.14.13.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="11">Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Mitochondrion outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the aromatic-ring hydroxylase family. KMO subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.14.13.9" evidence="11"/>
    <dbReference type="EMBL" id="FN430364">
        <property type="protein sequence ID" value="CAZ86043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841852.1">
        <property type="nucleotide sequence ID" value="XM_002841806.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNF0"/>
    <dbReference type="FunCoup" id="D5GNF0">
        <property type="interactions" value="715"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GNF0"/>
    <dbReference type="GeneID" id="9182243"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011259001"/>
    <dbReference type="eggNOG" id="KOG2614">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023210_2_1_1"/>
    <dbReference type="InParanoid" id="D5GNF0"/>
    <dbReference type="OMA" id="REFMFIA"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00328"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004502">
        <property type="term" value="F:kynurenine 3-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034354">
        <property type="term" value="P:'de novo' NAD+ biosynthetic process from L-tryptophan"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043420">
        <property type="term" value="P:anthranilate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070189">
        <property type="term" value="P:kynurenine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006569">
        <property type="term" value="P:L-tryptophan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019805">
        <property type="term" value="P:quinolinate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000129">
        <property type="entry name" value="Kynurenine 3-monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01971">
        <property type="entry name" value="Kynurenine_monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027545">
        <property type="entry name" value="Kynurenine_monooxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46028">
        <property type="entry name" value="KYNURENINE 3-MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46028:SF2">
        <property type="entry name" value="KYNURENINE 3-MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0274">FAD</keyword>
    <keyword evidence="2 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 11" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="8 11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6 11" id="KW-0521">NADP</keyword>
    <keyword evidence="7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 11" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="395"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="430"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding" evidence="13">
        <location>
            <begin position="7"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ86043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="460" mass="52151" checksum="BC9CC8FFD4DD1C5E" modified="2010-06-15" version="1">MDSLARKVIVVGAGPVGALAALYFSHAGWEVEVYELRGDLRLPENLSLNFSKSINLALSERGINGLRNLGDGGKLLDEVLSMTIPMYGRMIHTGVPGEKGESQKYDVRGRFIRSADRARLNVQLLDALVLKDVKLFFHHGLRGLNLDDRTAEFEMIGTGERVTVGADLIIGADGAHSATRRLLQKSVRMYFEQVYIETLWQEYEIPPDKETGDFKIDANHLHIWPKKDFMFIAIPSIDKTFTCTLFMEQWRFDAIGESRENLLAFFRENFEEVIDLVGEDSLVEQYLSHQRLPLISIKCRPYHYKDRCVIIGDAAHAMVPFYGQGMNAGFEDVSVLFEHIRRHPDSMADALASYSAYRMPDGHMINELAMQNHIEMRAAVTSKGYLFRKAIEEALYAYVPWLGVRTMYSMISFSNIRYTEVVNRTRRQRVWLNAVATALLVGGVMSGLWGANRLGLRKVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNV1</accession>
    <name>D5GNV1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00011522001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430370">
        <property type="protein sequence ID" value="CAZ86194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842003.1">
        <property type="nucleotide sequence ID" value="XM_002841957.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNV1"/>
    <dbReference type="GeneID" id="9182828"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011522001"/>
    <dbReference type="eggNOG" id="ENOG502S04M">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035511_0_0_1"/>
    <dbReference type="InParanoid" id="D5GNV1"/>
    <dbReference type="OMA" id="HEMLPCP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046921">
        <property type="term" value="F:alpha-(1-&gt;6)-fucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13132">
        <property type="entry name" value="ALPHA- 1,6 -FUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13132:SF29">
        <property type="entry name" value="ALPHA-(1,6)-FUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ86194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="466" mass="51794" checksum="3E803CBEEAA0630D" modified="2010-06-15" version="1">MPSPSQTNPRYRSAAEEPFLRQREKDDKLRRASPGLTRGRVFLIAITLSTMAWLLLVFQPSASPKPSKIDPASITSVEPTLLDYGEGNQKWTWKLPANLGGLLRPHVYSEMCRAARRASDVAAGHGRHAHHGYYWVDEHFLNPRVEDEAALRGVCERSLTYMLDSSDPGLGGMLLGLWSAYGLAMSENRTFFVDDSNWSWGTYATYFLPKKPTCRPPPPNLVIPCLNQVPHLLVAHSTTSATFGHAFTDFFEDARKMRVQRQHKIFALARSGYESLFRLRLSPADEEAVEVRKKQVKEERGNEKVLAVQIRRGDGKDKAFEWRGTGHVPVSRYADLISAASAAAGGNTTVLISSDDPAIYSLPEFVNYTPAQPVPSSPTTRSRLPGGWTRQTFENARASGSEAVTDLAREYFRDVKILGETASGLFCGGNGNTCRVLAVIMGWQTAVEEEGWVNVDDGRGWFGIDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>D5GP76</accession>
    <name>D5GP76_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00011741001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430373">
        <property type="protein sequence ID" value="CAZ86341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842150.1">
        <property type="nucleotide sequence ID" value="XM_002842104.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GP76"/>
    <dbReference type="GeneID" id="9186581"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011741001"/>
    <dbReference type="HOGENOM" id="CLU_1732823_0_0_1"/>
    <dbReference type="InParanoid" id="D5GP76"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072861" evidence="2">
        <location>
            <begin position="21"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="52"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ86341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="151" mass="16374" checksum="15B6BE9852D919E7" modified="2010-06-15" version="1">MKVSSTSILSLVLALNGVMASALQARGYEVVTVTELATEYVHVTSTAWVEPAQDAPTPIPTTAPDSHEKNKNNNHVTTDTPSTTLSILSMSESSRTTISTPSKPPTSTQLPFLPQYLCQHHSKTTHNIAKNLLLPPTTPVSNSRLQRRTDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPH5</accession>
    <name>D5GPH5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00011847001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430375">
        <property type="protein sequence ID" value="CAZ86418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842227.1">
        <property type="nucleotide sequence ID" value="XM_002842181.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPH5"/>
    <dbReference type="GeneID" id="9186658"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011847001"/>
    <dbReference type="HOGENOM" id="CLU_2414904_0_0_1"/>
    <dbReference type="InParanoid" id="D5GPH5"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ86418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="92" mass="10723" checksum="0E73B1F15EACD1A1" modified="2010-06-15" version="1">MFCRGETKGISVDFWTGLLLLLWSLSGRGLELYHTMLWSGLAQCSSLVVHGQRRSTARVHILRTYYRGILMHHVVRWVWNKLGIQRTLNVIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GQ55</accession>
    <name>D5GQ55_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00012204001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GTP cyclohydrolase I type 2/NIF3 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430382">
        <property type="protein sequence ID" value="CAZ86648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842457.1">
        <property type="nucleotide sequence ID" value="XM_002842411.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GQ55"/>
    <dbReference type="FunCoup" id="D5GQ55">
        <property type="interactions" value="626"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GQ55"/>
    <dbReference type="GeneID" id="9185228"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012204001"/>
    <dbReference type="eggNOG" id="KOG4131">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037423_0_1_1"/>
    <dbReference type="InParanoid" id="D5GQ55"/>
    <dbReference type="OMA" id="NFDKTHL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1390.30:FF:000001">
        <property type="entry name" value="GTP cyclohydrolase 1 type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1390.30">
        <property type="entry name" value="NIF3 (NGG1p interacting factor 3)-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002678">
        <property type="entry name" value="DUF34/NIF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036069">
        <property type="entry name" value="DUF34/NIF3_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00486">
        <property type="entry name" value="YbgI_SA1388"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13799">
        <property type="entry name" value="NGG1 INTERACTING FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13799:SF13">
        <property type="entry name" value="NIF3-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01784">
        <property type="entry name" value="DUF34_NIF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102705">
        <property type="entry name" value="NIF3 (NGG1p interacting factor 3)-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR602678-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ86648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="286" mass="31370" checksum="7996944FA26138D4" modified="2010-06-15" version="1">MSSNAIIRSRTLRTVVNAVQKLYPQALADRTWDNTGLLLEAPDRTDRSSPEAHNVLLTIDLTKAVVDEAISLRSSVIVAYHPIIFRPLKSLTLANTQQESLLRLAQEGISVYSPHTAVDAKIGGVNDWLADGISGGRGEESGRVVIEGIKDDSGMGRIITLKEPKGFQELVNRVKGFLGLKNVMVGKTPGDKRVRTIAICAGSGASMFKNVDVDLLFTGELSHHDALAAKERGVGFITCFHTNTERGFLRDVMKRKLAEVLESEVEREGEFEVVVSERDMDPFEIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5G3U8</accession>
    <name>D5G3U8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">mitochondrial processing peptidase</fullName>
            <ecNumber evidence="5">3.4.24.64</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Beta-MPP</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">GSTUM_00003811001</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalytic subunit of the essential mitochondrial processing protease (MPP), which cleaves the mitochondrial sequence off newly imported precursors proteins. Preferentially, cleaves after an arginine at position P2.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of N-terminal transit peptides from precursor proteins imported into the mitochondrion, typically with Arg in position P2.</text>
            <dbReference type="EC" id="3.4.24.64"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the peptidase M16 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.64" evidence="5"/>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835070.1">
        <property type="nucleotide sequence ID" value="XM_002835024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G3U8"/>
    <dbReference type="FunCoup" id="D5G3U8">
        <property type="interactions" value="844"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G3U8"/>
    <dbReference type="MEROPS" id="M16.003"/>
    <dbReference type="GeneID" id="9187129"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003811001"/>
    <dbReference type="eggNOG" id="KOG0960">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009902_4_2_1"/>
    <dbReference type="InParanoid" id="D5G3U8"/>
    <dbReference type="OMA" id="IDVVCDM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017087">
        <property type="term" value="C:mitochondrial processing peptidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006627">
        <property type="term" value="P:protein processing involved in protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000002">
        <property type="entry name" value="Mitochondrial-processing peptidase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000001">
        <property type="entry name" value="Mitochondrial-processing peptidase subunit beta, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050361">
        <property type="entry name" value="MPP/UQCRC_Complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001431">
        <property type="entry name" value="Pept_M16_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851:SF149">
        <property type="entry name" value="GH01077P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851">
        <property type="entry name" value="METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00143">
        <property type="entry name" value="INSULINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="16">
        <location>
            <begin position="56"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="17">
        <location>
            <begin position="209"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAZ79191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="480" mass="52721" checksum="5885A195534E25D7" modified="2010-06-15" version="1">MAAARLARSALTSSLRRNVIVPRKFPGITRSLASPAPVLIPGVTTQSTTLSNGLTIATEHSPYAQTATVGVFIDAGSRAETDKTNGTAHFLEHLAFKGTKSRTQGQLELEIEDMGGHLNAYTSRENTVYYAKSLKNDVGRSVEILADILQNSKLDESAIERERDVILREQEEVDKQLEEVVFDHLHATAFQGQPLGRTILGPKENILTISKGDLIDYISTNYKADRMVLTGAGGIPHETLVALAEKHFSGVKPSENPVTPGSARGPKPEFIGSEVRLRDDTIPTAHIAIAVEGVSWKDPHYFTALVAQAIIGNWDRAMSNAPYLGSKLSSFVHKHQLANSFMSFSTSYSDTGLWGIYLVTDKVTRIDDLVHFALREWSRLALTVTESEVERAKAQLKGSLLLSLDGTTAIAEDIGRQIITTGRRMNPAEVERVVGQITEQDVIDFAQRKIWDQDIAISALGSIEGLLDYQRVRNDTSRNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>D5G3X5</accession>
    <name>D5G3X5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00003845001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates histone H2A to form H2AS128ph (gamma-H2A) at sites of DNA damage, involved in the regulation of DNA damage response mechanism. Required for the control of telomere length and genome stability.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Associates with DNA double-strand breaks.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PI3/PI4-kinase family. TRA1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835097.1">
        <property type="nucleotide sequence ID" value="XM_002835051.1"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G3X5"/>
    <dbReference type="GeneID" id="9187156"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003845001"/>
    <dbReference type="eggNOG" id="KOG0889">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000129_2_1_1"/>
    <dbReference type="InParanoid" id="D5G3X5"/>
    <dbReference type="OMA" id="LVLDCAW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05163">
        <property type="entry name" value="PIKK_TRRAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050517">
        <property type="entry name" value="DDR_Repair_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003152">
        <property type="entry name" value="FATC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003151">
        <property type="entry name" value="PIK-rel_kinase_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014009">
        <property type="entry name" value="PIK_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046805">
        <property type="entry name" value="Tra1_ring"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139">
        <property type="entry name" value="ATAXIA TELANGIECTASIA MUTATED ATM -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139:SF1">
        <property type="entry name" value="TRANSFORMATION_TRANSCRIPTION DOMAIN-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02259">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02260">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20206">
        <property type="entry name" value="Tra1_ring"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51189">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51190">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAT" evidence="6">
        <location>
            <begin position="651"/>
            <end position="1198"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="5">
        <location>
            <begin position="1465"/>
            <end position="1790"/>
        </location>
    </feature>
    <feature type="domain" description="FATC" evidence="7">
        <location>
            <begin position="1788"/>
            <end position="1820"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="66"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ79218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1820" mass="207465" checksum="B3D9E4E145209B68" modified="2010-06-15" version="1">MTNIFQFLVKHGDLFYESREHFLSWIVTSLPKLVLHFPNPSTENKKLSINLIILIRDWEKRRISEAARTSPEASPSRKRKYGQLPADSRSPPAPGSVSNSDKTEYAVPTVLRMHVVKHLVTFICGLQEKFSSSELCWNALGLLKEFLSSRYWGDLDIDLFPKVTEKHLVLNDITKDTLPGFLNGLRVIGIIVDLKSDDWIMQHLPQIQKILEKSLRSDNAEVQKSLQPVLARVLQAIPATRVEGEEEEEEQTDSPSDSFISILTTIVQENLNTNLMSSVNILWTLSEKRPTVIDDHIPALMKAFQKLGKDHLSAPPGGPAPVVNLPNSAKTEPNTGGNSQVFDPDTTTELLRMVIDVASARVSVLGDQRRPFLSVMAQLVERSSSSELCLKILHIVRGWVFGSTESFPTLKEKIAVLSKMLSFESRPDPALCSSFLDLVIDIYEHPVITKTELTVRLEPAFLIGTRAKDVNMRNRFLQIFDRSLSRTISNRLSFVLASQNWEILADSYWLNQASHLLFGSVDMDLRIELHEDDLRVAPASSAVMAYVLDERRDSLMLDEKFEDFMARHRRFCQSLAEVRAGDIFEPMAQLQHLDPKVGHQIWVALFPLCWSALGKEDRQDLLRGIVSLLSKEWHHRQMDKRPNVIQSILEGVERASPKLTLPPHMVKFLSKTHDSCNLDALAEMYSMLSEEDLFYGLWRRRAQYLETNAAISYEQNGMWDKAQQMYEGAQIKARTGALPFSSSEYNLWEDHWVICAQKLQQWEILSEFAKHENYNDLLLECGWRLLENWNGADGERMDSTIKSLMDAPTPRRYFFAAFMALQKVHQKTEVPGEFSRLCDEAIQLSLRKWHNLPRRITNAHIPLLQAFQQLVELHDASVIYSSLSTTTAQNLDAKSQELKMLLGTWRDRLPNVWDDINSWRDLVTWRQHIFHSINNTYLPLLPGQTQSAGGSSNSSYAYRGYHETAWIINRFAHVARKHQLPEVCINQLSKIYTLPNIEIQEAFLKLREQAKCHYQNPNELTQGLEVINNTNLGYFGAGQKAEFYTLKGMFLSKLKQKEDANEAFGLALYHDLKLPKAWAEWGYYNDSLFRENPQDMQPAGNAVSCYLEAAGLYKSGKARKLLGRVLWLLSLDDASASISGAFDAYKGDIPIWYWITYIPQLLTSLSHKEARLARTVLIKIAKHFPQALYFLLRTSREDYQVIKKQTAQAAASNRGKAGSPIVGQAILSGRQTSVTIQTQANGIETVMASSPQSAISSGPQVIKAGSPAPVPTGQNMSSPTPAVPSPQPPVKRLPWEYAEEIMSVLKTAFPLLALSMETMGDQIQKFFKCPPDEDAYRLIVALLNDGLQWIARMNPSLEDSKLPPSTESNITRFAETILPAHIRVAFEADFVNERLNLFSYIERLRRWRDRFEEKLDRRPPNQNLEAYSPHLSEFRFQRFDDVEVPGQYLLHKDNNKDFVRIDRFLPRVDVVRGYGVCHRRLKLRGHDGSVHTFAIQHPAARHCRREERILQLFRIFNGALARRKESRRRNLGFHLPLMVPLAPHIRIVQDDASYISLQGIYEDHCRRTGMSKDDPLIFSLKKVIASLDPGRGGQQQVDANIKLEIFNSIQSTMVPPNIALEFMTKSYASFTEFFLFRRQFSYQYAALTFMTYVMNMGSRFPHKMFIARETGNIWGSELIPAMASTNAYFHNNESVPFRFSPNIQTLMGPIAIEGIFSCAVMAIARCLTEPEFELEQHLSVFVRDEMIFWFTQQHRSSIGEQQLRERVQANSEIIVRRATSLAQSSGAGGIPANQTVIDFISKASNPMNLAQLDNLWMPYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>D5G410</accession>
    <name>D5G410_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00003898001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835132.1">
        <property type="nucleotide sequence ID" value="XM_002835086.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G410"/>
    <dbReference type="STRING" id="656061.D5G410"/>
    <dbReference type="GeneID" id="9182380"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003898001"/>
    <dbReference type="eggNOG" id="ENOG502S1RX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040208_0_0_1"/>
    <dbReference type="InParanoid" id="D5G410"/>
    <dbReference type="OMA" id="RSNSTEW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025040">
        <property type="entry name" value="DUF3984"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13136">
        <property type="entry name" value="DUF3984"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="43"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="61"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="264" mass="29627" checksum="5FD65A13D842284F" modified="2010-06-15" version="1">MSLSPLTSGAVITEQDFCRKERRGREGSYIQGLSAPTTPGILSRSHSFSSRKSSYIDLPKSKSSSQLSHRSNSTEWLQRTGAALTSETREFKGQSWLVSRASSTSLVKDGDDDEIPDSFAATTTGITTPRRRPSRVYSNGGYMSEELEALERGNGYFDQLDDKADEQKEGEEEEIDIPKGFGLGQFVDRMIGWTVFAEDDAEDDESLDGYEEEALAEKASFRSQSVKFDTIDEEKLREQRRLRDEDMGWQDPAWVFSIASQVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4G2</accession>
    <name>D5G4G2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00004094001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835284.1">
        <property type="nucleotide sequence ID" value="XM_002835238.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4G2"/>
    <dbReference type="FunCoup" id="D5G4G2">
        <property type="interactions" value="26"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G4G2"/>
    <dbReference type="GeneID" id="9188378"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004094001"/>
    <dbReference type="eggNOG" id="ENOG502RYEC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018250_1_0_1"/>
    <dbReference type="InParanoid" id="D5G4G2"/>
    <dbReference type="OMA" id="FHGMQPH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003114">
        <property type="entry name" value="Phox_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22775">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22775:SF3">
        <property type="entry name" value="SORTING NEXIN-13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02194">
        <property type="entry name" value="PXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00313">
        <property type="entry name" value="PXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51207">
        <property type="entry name" value="PXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PXA" evidence="2">
        <location>
            <begin position="44"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="222"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ79405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="511" mass="57914" checksum="2DE94A547B27329D" modified="2010-06-15" version="1">MKPFRSSPVPAVDKSAIALIRRVLCYHAPPFTPLEQLLPALTSSPEVDLELYAFLAIIFRDFIYSWYSKITTDNAFADEIIAVVAHCSRAIEERVREVDLEALFLDEIPSFIENHVQDYRLALSRHSTPLSPHLAVREIFHNFQPHPALSSSPDSEKLYLKLVSSGILAVLLPTEDLESDCERSLVREVLSEMVLWNVVDRLSEPHIIHEIITNTLELLRTPMNPLSAPEPPPENERKPPVTHTRRKRSYSEKIPPIPLPHEPNGSVKNKIFQAPEVLAPPPANYKLIRIHPVMDTLLTIAFKVLSLLRTYLSSFITNLHSPPPPPPRPKKPLLRMSLFRLIPSYLHLPTLQPWTLSSLLFLTKPFTSRSTRLGLTLDNLLSSSFHSYIESPKLLVKILCSARSTLFPASPEPVRRTHPTPKEKARIKYTAEEAIWEATPSSIRKVYFGTDDKEEGLPVVGEMLLKTVQDKEVNKHLLYNLLDLTVGKLIPELTEEGPAELRRSRVGVVDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4J0</accession>
    <name>D5G4J0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00004170001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429987">
        <property type="protein sequence ID" value="CAZ79433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842513.1">
        <property type="nucleotide sequence ID" value="XM_002842467.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4J0"/>
    <dbReference type="GeneID" id="9184062"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004170001"/>
    <dbReference type="HOGENOM" id="CLU_2456391_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4J0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072207" evidence="2">
        <location>
            <begin position="24"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ79433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="89" mass="9181" checksum="30446D7B05350A7A" modified="2010-06-15" version="1">MKTLKYPLLLLLHPFAILAQGAGVPAPVCKDVDPGVTDYKLSLSPPTPAAPERGGVGEMRANRTTAADGEGMFRRVVGGIAVAVLVAVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4N4</accession>
    <name>D5G4N4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">O-acyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00000025001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Sterol O-acyltransferase that catalyzes the formation of stery esters.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN429989">
        <property type="protein sequence ID" value="CAZ79477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835320.1">
        <property type="nucleotide sequence ID" value="XM_002835274.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4N4"/>
    <dbReference type="STRING" id="656061.D5G4N4"/>
    <dbReference type="GeneID" id="9188413"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000025001"/>
    <dbReference type="eggNOG" id="KOG0380">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018190_2_0_1"/>
    <dbReference type="InParanoid" id="D5G4N4"/>
    <dbReference type="OMA" id="FICQMLQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097038">
        <property type="term" value="C:perinuclear endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034737">
        <property type="term" value="F:ergosterol O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008204">
        <property type="term" value="P:ergosterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140042">
        <property type="term" value="P:lipid droplet formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014371">
        <property type="entry name" value="Oat_ACAT_DAG_ARE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10408">
        <property type="entry name" value="STEROL O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10408:SF9">
        <property type="entry name" value="STEROL O-ACYLTRANSFERASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000439">
        <property type="entry name" value="Oat_ACAT_DAG_ARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="314"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="352"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="438"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="494"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="30"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="59"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000439-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ79477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="514" mass="59474" checksum="5F1EA1F170F3E10F" modified="2010-06-15" version="1">MESQPKISAFSASSSAGINHDPLENILRARSSNGAAQNLQELSNGEAHLKPVPARLESETSSVLSSGRSTPIPEDAPPSAHSIQHARRERRRRMFPTVDYESRVSHFDPQSQHRDFRGFFTLFWIGLFIMVLTTALRNLKDTRRVLRTSIFSMFTKAPLELAIADLFMFLSTIWSWGRSGYMLQHVLQAVWLAGWVHLPFHLEWQWTHQVFFTLHSLTMLMKMHSYSFYCGHLSECYKRLRTLDENNTKVEPTEDADLREALAFEVTSPSGRVTYPKNLTLYNFVDYLFCPTLCYELSYPRTDRIRWGKVAEKAAAVFGCIFLLTVTSEEFILPILAEAATRLESVNAYSDIALILLESISLLLFPFMVTFLLVFLVIFEYVLGAFAEITCFADRHFYSDWWNSTDWLEFSREWNIPVHRFLQRHVYGASRRHMSRPIATLTTFLISALAHELVMFCITKKLRGYGFVCQMLQLPIIVVQRTEFMKSQKMFNNIMFWSSMIWGLSMISALYVLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4R2</accession>
    <name>D5G4R2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00000062001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN429990">
        <property type="protein sequence ID" value="CAZ79498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835341.1">
        <property type="nucleotide sequence ID" value="XM_002835295.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4R2"/>
    <dbReference type="GeneID" id="9188434"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000062001"/>
    <dbReference type="eggNOG" id="KOG2913">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040201_4_0_1"/>
    <dbReference type="InParanoid" id="D5G4R2"/>
    <dbReference type="OMA" id="IDWNGAF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051415">
        <property type="entry name" value="LAAT-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201:SF37">
        <property type="entry name" value="PQ-LOOP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201">
        <property type="entry name" value="SEVEN TRANSMEMBRANE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00679">
        <property type="entry name" value="CTNS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="102"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="132"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="166"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="193"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="241"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="253"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ79498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="270" mass="29495" checksum="B7F0849D56876EC9" modified="2010-06-15" version="1">MPAQESIPPAANVLGTIGTVLWCIQLIPQIWFNYRNKSTEGLPGIMMLVWGFAGVPFGAYAVIQNLNIPIQIQPQVFASLSLISWAQCLHYSSGWAVWSSSLIALAVAIACAGTEVALILTLQPIYDRGTEWPMLVVGIIASILLAAGLIPPYFEIWKRRGRVVGIDFVFLSIDWLGALFSIMSLVAQNTFDYLGGVLYLVVILLESGIFISHWVWLFRTRKQRKAEKEAGAISTVATALSESVGEKPHGSDMASTAGSNNQEVELKQDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4T3</accession>
    <name>D5G4T3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00000182001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN429991">
        <property type="protein sequence ID" value="CAZ79526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835369.1">
        <property type="nucleotide sequence ID" value="XM_002835323.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4T3"/>
    <dbReference type="GeneID" id="9183008"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000182001"/>
    <dbReference type="eggNOG" id="KOG2615">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_54_6_1"/>
    <dbReference type="InParanoid" id="D5G4T3"/>
    <dbReference type="OMA" id="FTWLWQF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17330">
        <property type="entry name" value="MFS_SLC46_TetA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001958">
        <property type="entry name" value="Tet-R_TetA/multi-R_MdtG-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504:SF39">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G03860)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01035">
        <property type="entry name" value="TCRTETA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="146"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="206"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="246"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="358"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="398"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="432"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="462"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="510"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="536"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="75"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="304"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="561"/>
            <end position="580"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ79526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="580" mass="61004" checksum="E86FB42DB9E025AE" modified="2010-06-15" version="1">MQRPKSPVHYQTFPTTPPASRGAHVGGNSGPPESSLESPSDDRSSVSKASAGTSGKAGGGRGNSDDIQSTIPKKQLLTLAIISLAEQTALNSISPYLPEMTGAFPEVDQRRVGLYVGIVASSFATAQFATNFFWGRLSDRIGRKPVILFGTLATAACFMAFGFCRTLWQAIVVQAIMGLVNGNAGIVSTILGEITDKSNQPSTFKYLPVMYGIGGITGPLIGGVLAHPVTSEPSGPITKLFGKNPYLLPNAVSAGVLLLDLLFATFLLDESLQEARKLPPLGERVKNLFVWLWEFTSSSRPSYIRVSQSHRDESDDEGEGAQDSPPHPTDCSGLPSPPPLFPEATAPVPYSSILTPPIIVFLITYAIFNLSNTSYNSLYPIFLSSPPPTGRGLSPKELGLSLSFAGVVTIIFELLLFGPLQSHLGNLWGYRFALAGFVAAFCATPFVGYIDPANERSTKAWLWIELCSILLLKTVAAVVGLTCALLLITNASPKSSTLGTLNGLAQTLNAGGRAIGPLISGGLFTLGAGLPNRGELLAWGVFGGVSGIGLGLSLRGVGGERSLDGEGGEEGNFEGGAEMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4Y4</accession>
    <name>D5G4Y4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Diphthamide biosynthesis protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00000154001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2 [3Fe-4S](0)-[protein] + 2 Fe(2+)-[Dph3] + NADH = 2 [4Fe-4S](1+)-[protein] + 2 [Dph3] + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:71239"/>
            <dbReference type="Rhea" id="RHEA-COMP:17997"/>
            <dbReference type="Rhea" id="RHEA-COMP:17998"/>
            <dbReference type="Rhea" id="RHEA-COMP:18001"/>
            <dbReference type="Rhea" id="RHEA-COMP:18002"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:33723"/>
            <dbReference type="ChEBI" id="CHEBI:47402"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:83228"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[3Fe-4S](1+)-[protein] + Fe(2+)-[Dph3] = [3Fe-4S](0)-[protein] + Fe(3+)-[Dph3]</text>
            <dbReference type="Rhea" id="RHEA:71235"/>
            <dbReference type="Rhea" id="RHEA-COMP:17996"/>
            <dbReference type="Rhea" id="RHEA-COMP:17997"/>
            <dbReference type="Rhea" id="RHEA-COMP:18002"/>
            <dbReference type="Rhea" id="RHEA-COMP:18003"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:33751"/>
            <dbReference type="ChEBI" id="CHEBI:47402"/>
            <dbReference type="ChEBI" id="CHEBI:83228"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the DPH3 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429993">
        <property type="protein sequence ID" value="CAZ79577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835420.1">
        <property type="nucleotide sequence ID" value="XM_002835374.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4Y4"/>
    <dbReference type="FunCoup" id="D5G4Y4">
        <property type="interactions" value="326"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G4Y4"/>
    <dbReference type="GeneID" id="9183059"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000154001"/>
    <dbReference type="eggNOG" id="KOG2923">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_155991_4_1_1"/>
    <dbReference type="InParanoid" id="D5G4Y4"/>
    <dbReference type="OMA" id="IYDPDMF"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.660.10:FF:000001">
        <property type="entry name" value="Diphthamide biosynthesis 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.660.10">
        <property type="entry name" value="DPH Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044248">
        <property type="entry name" value="DPH3/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007872">
        <property type="entry name" value="DPH_MB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036671">
        <property type="entry name" value="DPH_MB_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21454:SF31">
        <property type="entry name" value="DIPHTHAMIDE BIOSYNTHESIS PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21454">
        <property type="entry name" value="DPH3 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05207">
        <property type="entry name" value="Zn_ribbon_CSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144217">
        <property type="entry name" value="CSL zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51074">
        <property type="entry name" value="DPH_MB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DPH-type MB" evidence="9">
        <location>
            <begin position="5"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00036267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00041070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ79577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="87" mass="9681" checksum="065CB6519098B21D" modified="2010-06-15" version="1">MTDSIYDEIEIEDMTFDKDRGLYTYPCPCGDRFEILLDDLRDGEEIAVCPSCSLMIRVIFEAGDLPRVEKEGLKGKMEAAVAVAVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4Z5</accession>
    <name>D5G4Z5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00000171001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS9 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429993">
        <property type="protein sequence ID" value="CAZ79588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835431.1">
        <property type="nucleotide sequence ID" value="XM_002835385.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4Z5"/>
    <dbReference type="FunCoup" id="D5G4Z5">
        <property type="interactions" value="713"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G4Z5"/>
    <dbReference type="GeneID" id="9183070"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000171001"/>
    <dbReference type="eggNOG" id="KOG1753">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046483_4_0_1"/>
    <dbReference type="InParanoid" id="D5G4Z5"/>
    <dbReference type="OMA" id="WPIEMAR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000007">
        <property type="entry name" value="40S ribosomal protein S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000754">
        <property type="entry name" value="Ribosomal_uS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020574">
        <property type="entry name" value="Ribosomal_uS9_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001749">
        <property type="entry name" value="PRK00474.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569:SF16">
        <property type="entry name" value="RIBOSOMAL PROTEIN S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569">
        <property type="entry name" value="RIBOSOMAL PROTEIN S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00380">
        <property type="entry name" value="Ribosomal_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00360">
        <property type="entry name" value="RIBOSOMAL_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ79588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="144" mass="16110" checksum="2CA1CD5C8C179A22" modified="2010-06-15" version="1">MATPTQSVQCFGKKKTATAVAHCKKGRGLVKVNGSPLNLVKPEILRFKVYEPLLIVGLDRFAEVDIRVRVSGGGHTSQVYAIRQAIAKSIVAYYQKFVDEHSKNQLKQALITYDRSLLVADNRRCEPKKFGGPGARARYQKSYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5G561</accession>
    <name>D5G561_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 12">Histone H2A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">GSTUM_00000269001</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">Mel28</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4 12">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the histone H2A family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429996">
        <property type="protein sequence ID" value="CAZ79646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835489.1">
        <property type="nucleotide sequence ID" value="XM_002835443.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G561"/>
    <dbReference type="FunCoup" id="D5G561">
        <property type="interactions" value="927"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G561"/>
    <dbReference type="GeneID" id="9186344"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000269001"/>
    <dbReference type="eggNOG" id="KOG1756">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062828_3_0_1"/>
    <dbReference type="InParanoid" id="D5G561"/>
    <dbReference type="OMA" id="CALESQH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00074">
        <property type="entry name" value="HFD_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000008">
        <property type="entry name" value="Histone H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002119">
        <property type="entry name" value="Histone_H2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032454">
        <property type="entry name" value="Histone_H2A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032458">
        <property type="entry name" value="Histone_H2A_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23430">
        <property type="entry name" value="HISTONE H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16211">
        <property type="entry name" value="Histone_H2A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00620">
        <property type="entry name" value="HISTONEH2A"/>
    </dbReference>
    <dbReference type="SMART" id="SM00414">
        <property type="entry name" value="H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00046">
        <property type="entry name" value="HISTONE_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6 12" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0488">Methylation</keyword>
    <keyword evidence="11 12" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="10 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="14">
        <location>
            <begin position="14"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="Histone H2A C-terminal" evidence="15">
        <location>
            <begin position="93"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF16211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAZ79646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="134" mass="14022" checksum="582E2DF13AF61D58" modified="2010-06-15" version="1">MSSGGKGGKAASGKPSQSRSSKAGLAFPVGRVHRLLRKGNYAQRVGAGAPVYLAAVLEYLAAEILELAGNAARDNKKTRIIPRHLQLAIRNDEELNKLLGHVTIAQGGVLPNIHQNLLPKKSGKGGAKGASQEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5A8</accession>
    <name>D5G5A8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00004250001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_002835498.1"/>
    </dbReference>
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    <dbReference type="KEGG" id="tml:GSTUM_00004250001"/>
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    <dbReference type="InParanoid" id="D5G5A8"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ79701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="82" mass="9559" checksum="8A5D076F6C0945E2" modified="2010-06-15" version="1">MFLNICQQLCRIEAIFFRLLPLLPCPSHLSCLEHPRYQRFDHPQPYALLTVIGISYFLLRQLCSLLVSAGLIFYPNIHKKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
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    <name>D5G5F3_TUBMM</name>
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        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
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        <name evidence="2" type="ORF">GSTUM_00004309001</name>
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        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
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        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
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            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="CAZ79746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002835543.1"/>
    </dbReference>
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    <dbReference type="STRING" id="656061.D5G5F3"/>
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    <dbReference type="eggNOG" id="ENOG502QT51">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_376908_0_0_1"/>
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    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="313"/>
        </location>
    </feature>
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        </location>
    </feature>
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            <end position="711"/>
        </location>
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            <begin position="33"/>
            <end position="44"/>
        </location>
    </feature>
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            <end position="69"/>
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            <end position="108"/>
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            <begin position="121"/>
            <end position="135"/>
        </location>
    </feature>
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            <begin position="173"/>
            <end position="184"/>
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            <end position="208"/>
        </location>
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            <begin position="209"/>
            <end position="220"/>
        </location>
    </feature>
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            <end position="272"/>
        </location>
    </feature>
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            <end position="685"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="686"/>
            <end position="705"/>
        </location>
    </feature>
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        </source>
    </evidence>
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            <dbReference type="EMBL" id="CAZ79746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="736" mass="79932" checksum="D00B40499BA19E78" modified="2010-06-15" version="1">MPTLPSFLSKHNRTSATPTPPPSSASPRPTISGPLQQAYSSLSRHSSHGGATVQLVPSDSRSAAEQQEANNPSSSSSLSSYNPSSRKPLENNNAAGNNNNPGGSNHGSRLPAGLSRRLSRHQSTPPSFPQQQKQQTESDHLDPYLQQSPHSPGPPPHSPGRPQSMLAPPQESEGEHYSVKRADTDPAQQQYSQHPHPQRQDSFQNPANLQVQLQQQQQSQGNHAMANQAHNQPRRSMEQSNQVGPSREASALSQMNVYASSGRGNPNQQDYQIQAGGGTGGNQPLAYDPAKYEGQPMGRMTPENRAESRSGSAPEAINVPQLLQDHETLTAKYRKVKNLYFESKSEIERLQNTLAHQRISQSRTSLDDNEYANRFDRLDGAIKNVAFEIRQSWKAIPSWLGGVINSGAEVKGGREMTVVGRACISKWVVDEILEKFFHPALDANLSAQLKMVEHGIRINAPPLQSIEDDDALSAKVCNWRLTTLDGLRDSIHGPHAMEYRQRLSEHLVKQLAESLQMHLHEPSPPGLLGGIQMIVDIAVGLGANLPLESRDVRVWYPLPGERFDSKFMRGEVGLPPLVTPTRGLEGEGEKLQLDTNQQSHIMESCETSPLPPPAPAKDDKPLPAGKAGIGALPPPTGAMQNKEGAGKKLAGLNRVKKAFQGTAHQPTSPNLSPSTGQQGLQHNGNSGSQASLSQQQQPQPAQDPTAPKDGDRVRLAGFMACEVRGKLILVKAPVWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5L9</accession>
    <name>D5G5L9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00001479001</name>
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    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
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            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="STRING" id="656061.D5G5L9"/>
    <dbReference type="GeneID" id="9187517"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001479001"/>
    <dbReference type="eggNOG" id="KOG1364">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="HOGENOM" id="CLU_021255_2_0_1"/>
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    <dbReference type="OMA" id="PAIFDCQ"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02958">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="UBA_p47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01767">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
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    </dbReference>
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        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
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    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
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    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
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    <dbReference type="InterPro" id="IPR050730">
        <property type="entry name" value="UBX_domain-protein"/>
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    <dbReference type="PANTHER" id="PTHR23322">
        <property type="entry name" value="FAS-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322:SF6">
        <property type="entry name" value="UBX DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13899">
        <property type="entry name" value="Thioredoxin_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14555">
        <property type="entry name" value="UBA_4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00594">
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        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00166">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS50033">
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    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBX" evidence="2">
        <location>
            <begin position="437"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="89"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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    <feature type="compositionally biased region" description="Pro residues" evidence="1">
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <end position="381"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="532" mass="58588" checksum="50300B72F4D4ADA1" modified="2010-06-15" version="1">MDELISQFTTITGATVRKAETYLKVSDGDLEQAIQLYFDTGGADMESGPSTQTAPPPPPPPPAEALRASEPTRSTATPGSGSGSGVNSAYDEDEAIARRLQEELYGEAGGASSGGENGVRAPIARTRDTLIGGDDYGYPEGIAERRRLRAATSRFGAFGRRSAANVWAPESTDSSESLLEAVGGVSESSSKPSKLAEIFRPPFEIMTNLSFQDARDEAKEVEKWILVNIQDNSIFSCQLLNRDIWKAPEVKATVKENFVFLQMDRAGRDGKDYLRLYMANAVDDTALFSSGTKAEDVFPHIAIIDPRTGEQVKVWTDVPKNPLEFLMVLHEFLDRYSLKVDAKNPVQRKTKPKASVAHMTEDEMMQLAMQNSLGGTSTPLGATSDPDDLTKTGGASSDVGDLMEFEEVQENQADTSEKQESVFWRIRGDKHHAEPLSGPDVTRIQFKMSDGTRVVRRFLLKDRVERLFEYVKADLLPEHQEKRKSGPDDKEDLLVGKEFELVSLGKKLIDHLEETIDEAGLKMGTVMVEVLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5W7</accession>
    <name>D5G5W7_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nucleolar protein 12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00001604001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in pre-25S rRNA processing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RRM RBM34 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430002">
        <property type="protein sequence ID" value="CAZ79910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835753.1">
        <property type="nucleotide sequence ID" value="XM_002835707.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5W7"/>
    <dbReference type="FunCoup" id="D5G5W7">
        <property type="interactions" value="733"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G5W7"/>
    <dbReference type="GeneID" id="9187588"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001604001"/>
    <dbReference type="eggNOG" id="KOG0118">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006468_3_0_1"/>
    <dbReference type="InParanoid" id="D5G5W7"/>
    <dbReference type="OMA" id="NAYAVYT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12670">
        <property type="entry name" value="RRM2_Nop12p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047189">
        <property type="entry name" value="RRM2_Nop12p-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF25">
        <property type="entry name" value="RNA-BINDING PROTEIN 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="7 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="275"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="38"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="345"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="416"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="85"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="144"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="429"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ79910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="453" mass="50191" checksum="C2C93C44AD2709F6" modified="2010-06-15" version="1">MGKKSRWEKSSATAPSCSNIDPSLASLFDLSFGAVQVPQKASIESTRSNKAAEGEPEEDDEDLSEVEGSDLEDTSDGEAANDSQSGKDDDTKDLRATTAPGDKRQKRKRRSDKGEIEDIYLQKLQDSQDAEDKRRMSKRTRTEAEDEEFRESDEDDIDVEKEEPSAKHESLANPGDVELDKSSRTVFLGNVPSAAISSKIISIRFRSVAFSEQIPRKAAFVTHKLHEKQQTVNAYVVYSTPVEAREALKLNGKVVLDRHIRVDSVAHPSPQDPKRCVFIGNLDFEAQEENLWRHFGTCGKVESVRVVRDAKTNVGKGFAYVQFEDPMSVDEALLLDSKKMANDRKLRVSRAKTIKRNQTGKPDPPLSDKRSSRSKGVYIPKLDPKTKNIIGRAHKLLGRAGAAQLKSQSEVFEGLRASANTDSGIKKGGSGKKTGKPRSRARAAAWKQKGNHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6I5</accession>
    <name>D5G6I5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00004495001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430009">
        <property type="protein sequence ID" value="CAZ80128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835971.1">
        <property type="nucleotide sequence ID" value="XM_002835925.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6I5"/>
    <dbReference type="STRING" id="656061.D5G6I5"/>
    <dbReference type="GeneID" id="9181213"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004495001"/>
    <dbReference type="eggNOG" id="ENOG502RYEB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_774315_0_0_1"/>
    <dbReference type="InParanoid" id="D5G6I5"/>
    <dbReference type="OMA" id="IHIYEPV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23659">
        <property type="entry name" value="USP_At3g01520-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47815">
        <property type="entry name" value="UNIVERSAL STRESS PROTEIN A FAMILY PROTEIN C25B2.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47815:SF1">
        <property type="entry name" value="UNIVERSAL STRESS PROTEIN A FAMILY PROTEIN C25B2.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="108"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="244"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="40"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="270"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="332"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ80128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="358" mass="38823" checksum="EFE633C2103BF927" modified="2010-06-15" version="1">MTSPSTATAPLAEKEVRPTSIAWRANLPERPASEGRRRQLSLASSVGSVSLPSGSVRGDRPRSLSSPPPPPTFLKRVSFDTFDNKDASDFSLTLKSKHKDYAYTRRSRTFLCGTDQNDYSEFALEWLIDELVDDGDEVVCLRVVDKDSKISSDAALQVRQYRHEAENLLNQIIEKNEDDKTISIILEFAVGKVHETIQKMIHIYEPVILIVGTRGRSLGGLQGLLPGSVSKYCLQHSPVPVIVVRPSSKRDKKKAKRKADPGRKVYQEMVHNSDTAFTNHGNHSHRSVSGVTEAMSNTSISSLGEPIGQNSPGGDVSPKSLAPDGGGEGGNSPASESPTPSSECFGGGGAKLDVEEDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6Q0</accession>
    <name>D5G6Q0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Proteasome subunit alpha type</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00002160001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the peptidase T1A family.</text>
    </comment>
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        <property type="protein sequence ID" value="CAZ80193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002835990.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6Q0"/>
    <dbReference type="FunCoup" id="D5G6Q0">
        <property type="interactions" value="1229"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G6Q0"/>
    <dbReference type="GeneID" id="9187219"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002160001"/>
    <dbReference type="eggNOG" id="KOG0184">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035750_0_1_1"/>
    <dbReference type="InParanoid" id="D5G6Q0"/>
    <dbReference type="OMA" id="RVSMYMH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034515">
        <property type="term" value="C:proteasome storage granule"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="entry name" value="proteasome_alpha_type_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000007">
        <property type="entry name" value="Proteasome subunit alpha type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0539">Nucleus</keyword>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="7">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002000"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ80193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="285" mass="30746" checksum="F058493EBEC27F90" modified="2010-06-15" version="1">MPSSVLQPLPLQESIHHFPVMTSIGTGYDLSNSVFSPDGRNFQVEYATKAVENGGTAIGIRCKDGVVLAVEKLIGSKLLVLGSNKRIASVDSHIGIACSGLIPDARHFTSRARSEASNWRKLYRSPIPISSMADAMGGYAQAYTLYSSVRPFGVTAIIGGMDEAGGPGLWMVEPSGVGWGYFGAATGRGRQAAKAELEKLPLPNITVEEAVRQAARIIYFAHDDNSKDKDFELEMTWVRLEKGSAEDAAVKGWGKHEEVPKELVAEAERLAKESMEGGGDEEMEE</sequence>
</entry>
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    <accession>D5G6S6</accession>
    <name>D5G6S6_TUBMM</name>
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        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
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    </gene>
    <organism evidence="2 3">
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        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
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            <taxon>Fungi</taxon>
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            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
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            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430014">
        <property type="protein sequence ID" value="CAZ80219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="GeneID" id="9187244"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002248001"/>
    <dbReference type="HOGENOM" id="CLU_2814276_0_0_1"/>
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    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="67" mass="7822" checksum="1011D78907965914" modified="2010-06-15" version="1">MAQFLFTSGSCRIYRIAHPDPKSGKRKQNKKEKKPSHNPRQSLTFSHSSSLKKKEREKKATQKKTHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5G700</accession>
    <name>D5G700_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00004524001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430020">
        <property type="protein sequence ID" value="CAZ80293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836102.1">
        <property type="nucleotide sequence ID" value="XM_002836056.1"/>
    </dbReference>
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    <dbReference type="FunCoup" id="D5G700">
        <property type="interactions" value="85"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G700"/>
    <dbReference type="GeneID" id="9182484"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004524001"/>
    <dbReference type="eggNOG" id="ENOG502QSKI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019711_0_0_1"/>
    <dbReference type="InParanoid" id="D5G700"/>
    <dbReference type="OMA" id="IEAYQHC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034044">
        <property type="term" value="C:exomer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000149">
        <property type="entry name" value="Clathrin-coated vesiclec protein (Bud7)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015374">
        <property type="entry name" value="ChAPs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31975">
        <property type="entry name" value="BUD SITE SELECTION PROTEIN 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31975:SF1">
        <property type="entry name" value="BUD SITE SELECTION PROTEIN 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09295">
        <property type="entry name" value="ChAPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ80293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="611" mass="69618" checksum="103ECCBDF8B10009" modified="2010-06-15" version="1">MVAPAVPEIVESEIHESVNTRTESLPILRELGPPDLVHLTKSQPKVGLKEIGTYHHVTGVDASSSASLAAYINTLTYLIGENQIWFGKPQSWRITTGIYCCYNAFSRVDMRVEVKIPGSVEAYAVDERGEKRMASELLWLETYLCGVLRAFNYADDQQNRIVGCRRFNPITNTEAEHKFLDAAERLFSRGWQLGSDPEIQVPNMVSNYLTSGLLTYIHTTGRFASGVNLFEKLRSKEPEVASLLARVLIDADEEVKAVSLLCETIKLLPLDSGLLDVQAAFCMSKGRYDLALECAKKAVNSAPSEFTTWERLAQVYLKLEQYELALLTLNSCPMFTYQDRDHPKMPQPTKMHLPVLAESVLDEMSDESHDPKHDKLDAALLKLPAASLRGTFAKAYKLLTEITSQIGWDMLLKYRSNVFVMEEEYRFEKSARRISTQKYSHIQNKRLCERWLDNLFMVLYEDLRVYTIWRSDLVRHGQQKRPYRKSATELEILGELAQRLHHHAEAEEAFQSRLQIRFSARALRGVLQTQEKNKDNRGALNSIIRLTAWQYRWYSEFSPAILYSIRKLIAEDGAVKVRSIVQATNFPQSVLDLTHNYAQLCAAFRSSGSDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7E4</accession>
    <name>D5G7E4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00002192001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430025">
        <property type="protein sequence ID" value="CAZ80437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836246.1">
        <property type="nucleotide sequence ID" value="XM_002836200.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7E4"/>
    <dbReference type="GeneID" id="9188462"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002192001"/>
    <dbReference type="HOGENOM" id="CLU_3191638_0_0_1"/>
    <dbReference type="InParanoid" id="D5G7E4"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ80437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="46" mass="4623" checksum="56FB0E751EE28818" modified="2010-06-15" version="1">MFTEQGFHIVSVAADMIVTGGFLSAALDNPRGAQGGTGISSGPYGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7Q6</accession>
    <name>D5G7Q6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00004681001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the eukaryotic/archaeal RNase P protein component 4 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430031">
        <property type="protein sequence ID" value="CAZ80549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836358.1">
        <property type="nucleotide sequence ID" value="XM_002836312.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7Q6"/>
    <dbReference type="FunCoup" id="D5G7Q6">
        <property type="interactions" value="8"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G7Q6"/>
    <dbReference type="GeneID" id="9187028"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004681001"/>
    <dbReference type="eggNOG" id="KOG4394">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079140_1_0_1"/>
    <dbReference type="InParanoid" id="D5G7Q6"/>
    <dbReference type="OMA" id="REGECNV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005655">
        <property type="term" value="C:nucleolar ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007175">
        <property type="entry name" value="Rpr2/Snm1/Rpp21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14742:SF0">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14742">
        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04032">
        <property type="entry name" value="Rpr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="120"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ80549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="165" mass="17647" checksum="7A5EF558A2316969" modified="2010-06-15" version="1">MAHKKPANSAQAPPRPHLFARLNYLHQSAHLLLTSGSPNLSRNLVSTARTVAKKTVIRFDPSVKRTWCKRCDAVLVPGVSCEHRVENLSRGGRKAWADVLVVRCWGCGCLKRFPVGRGGRRGESGGNGEGGKAREGECNVAGEGKGKGKKVRVKVKGGEVGDPTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7V7</accession>
    <name>D5G7V7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00002672001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430032">
        <property type="protein sequence ID" value="CAZ80600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836409.1">
        <property type="nucleotide sequence ID" value="XM_002836363.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7V7"/>
    <dbReference type="FunCoup" id="D5G7V7">
        <property type="interactions" value="34"/>
    </dbReference>
    <dbReference type="GeneID" id="9181419"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002672001"/>
    <dbReference type="eggNOG" id="ENOG502QSAP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042679_1_1_1"/>
    <dbReference type="InParanoid" id="D5G7V7"/>
    <dbReference type="OMA" id="AINCWAS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="32"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="405" mass="45503" checksum="9C9188F1A36A64DD" modified="2010-06-15" version="1">MRISGVGILDSQEDPSILDATHQTSTKSPSLSLPSELIYLVLDFLSPQDQKTLAAVCKINRQWYFAATPYLYRHPQITSVNYTKFVATIAPSSTHVKPSRFAGLVKRIDLSRIVHDSTPSSIARLLRRTQGSLEEFVAPQATFGYIALVAVGNCKQLRLLNLSLVSQSIALETLFQHIQDLPHLVTLYFPRSSTVDRCKSTFNWPPRLKRFSLAGRIGDSFLINTCLPPTLHELQIAHCPFVKDTSIKYLLNKASHQLTVLSINYHIPCLGFNAFDKVLIICPHLEKLTIAVDYVSAHLFDEENSPSGHPLRWLNLESSGNPGVEKKITPDDVFIAVAEERLSELRVVRASKRLGWEQENSKSMMMDLNELLETRAEEKGETGAGVWNFESEYSCKSEAFRGFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>D5G817</accession>
    <name>D5G817_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00002706001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430038">
        <property type="protein sequence ID" value="CAZ80660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836469.1">
        <property type="nucleotide sequence ID" value="XM_002836423.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G817"/>
    <dbReference type="STRING" id="656061.D5G817"/>
    <dbReference type="GeneID" id="9184132"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002706001"/>
    <dbReference type="eggNOG" id="ENOG502RYJ5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010103_1_0_1"/>
    <dbReference type="InParanoid" id="D5G817"/>
    <dbReference type="OMA" id="HDIHASE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009836">
        <property type="entry name" value="GRDP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34365">
        <property type="entry name" value="ENOLASE (DUF1399)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34365:SF7">
        <property type="entry name" value="GLYCINE-RICH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07173">
        <property type="entry name" value="GRDP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="407"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="410"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="444" mass="50149" checksum="E65A67B50F9917E1" modified="2010-06-15" version="1">MPPLDVLMVWHAFILNPRCFLEDCYRVGKIDFWRAGLPWSVIDSLIDNGFDYNLPITAINNFQTSTNLSVENLDFPDSLPVNCPICSNLVPILITNSRDSTGYADSKFSKTCPNGTCQAVINIDTLSRAKFLQALDRLTSPAGTPLPGTILDKDGKPNPEIMLPDKSWMFPNQFCIFLQSSLRTCGSNPPTADLNDIKVEMNRSLENRKFLKSGIVPYRHLDRDGKISFRRMMSRFWSNPSPFSIHLVGAVIRQGTFVEKMAEIDWLHSPAIVDTIEAAVKKFERFMDMIKNHPGRCVVPTLNVDLVWHTLMGNPSIYWKYSLALTGKFVGHDDKIDEAKLGEAFKWTCEKYEKLYREPYSECMPPLSDPSVLHNLADMTRHLLVLRSYPLPLPIHDETLLLLKAPPNTRRSRSKHAHIRPLSSPSPRQAPEGAAESLLPGSDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5G819</accession>
    <name>D5G819_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="13">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00002715001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>Fe(II)-heme o + 2 A + H2O = Fe(II)-heme a + 2 AH2</text>
            <dbReference type="Rhea" id="RHEA:63388"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:60530"/>
            <dbReference type="ChEBI" id="CHEBI:61715"/>
            <dbReference type="EC" id="1.17.99.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:63389"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="10">Porphyrin-containing compound metabolism; heme A biosynthesis; heme A from heme O: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430038">
        <property type="protein sequence ID" value="CAZ80662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836471.1">
        <property type="nucleotide sequence ID" value="XM_002836425.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G819"/>
    <dbReference type="FunCoup" id="D5G819">
        <property type="interactions" value="841"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G819"/>
    <dbReference type="GeneID" id="9184134"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002715001"/>
    <dbReference type="eggNOG" id="KOG2725">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017627_4_2_1"/>
    <dbReference type="InParanoid" id="D5G819"/>
    <dbReference type="OMA" id="AFVCYSW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120547">
        <property type="term" value="F:heme A synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016653">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, heme protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006784">
        <property type="term" value="P:heme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01665">
        <property type="entry name" value="HemeA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003780">
        <property type="entry name" value="COX15/CtaA_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023754">
        <property type="entry name" value="HemeA_Synthase_type2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23289">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23289:SF2">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX15 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02628">
        <property type="entry name" value="COX15-CtaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="195"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="222"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="263"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="319"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="391"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="431"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="457"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ80662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="512" mass="55601" checksum="5C7B47B86555B03B" modified="2010-06-15" version="1">MSFANLTPRGISANICRTGLSRTTTLGLPRSLLAIERSKSASPLMLPRVRNFSFTNTSRNKAATPAPATHPGTHSSPLGTLANTFAHNSQTLKAGTKTQPFFPDISSPTVGYWLLGSAGLVFGIVVLGGLTRLTESGLSITEWKPITGTLPPLSPEDWESNFSKYRNSPEYCILNPNMTLDEYKFIYYMEWTHRLLGRMIGLFFVLPAIYFVARGRVSRRMAFGLGGISGLIALQGGIGWWMVKSGLKDELFQQPGAHPRVSQYRLTAHLGTAFAVYAAMLWNGLSVLRENRLLLPNPKAAIDEFNALSNPVLRPFRRLVGVIGVLVFITAMSGGLVAGLDAGLIYNEFPKMGNGYIPPLTELMDPFYAQHPDHSDTWWRNMLENPTTVQFDHRCLAISTFSFIAAVFAYSRLSPAVAGALTKNGKGGITGVMHLAILQVLLGVGTLVYMVPTHLAATHQAGSLALFTGAIVLASRVWLPRAAASAARRRLEVMAGRKVGGGEGREARRGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5G831</accession>
    <name>D5G831_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00002787001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SAM50/omp85 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430039">
        <property type="protein sequence ID" value="CAZ80674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836483.1">
        <property type="nucleotide sequence ID" value="XM_002836437.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G831"/>
    <dbReference type="FunCoup" id="D5G831">
        <property type="interactions" value="132"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G831"/>
    <dbReference type="GeneID" id="9184147"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002787001"/>
    <dbReference type="eggNOG" id="KOG2602">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014798_3_1_1"/>
    <dbReference type="InParanoid" id="D5G831"/>
    <dbReference type="OMA" id="KHPVARF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045040">
        <property type="term" value="P:protein insertion into mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.50:FF:000008">
        <property type="entry name" value="Mitochondrial outer membrane beta-barrel protein Tob55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.50">
        <property type="entry name" value="membrane protein fhac: a member of the omp85/tpsb transporter family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000184">
        <property type="entry name" value="Bac_surfAg_D15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039910">
        <property type="entry name" value="D15-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12815:SF18">
        <property type="entry name" value="SORTING AND ASSEMBLY MACHINERY COMPONENT 50 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12815">
        <property type="entry name" value="SORTING AND ASSEMBLY MACHINERY SAMM50 PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01103">
        <property type="entry name" value="Omp85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1134">Transmembrane beta strand</keyword>
    <feature type="domain" description="Bacterial surface antigen (D15)" evidence="6">
        <location>
            <begin position="174"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ80674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="506" mass="54674" checksum="296B7E893CB1C3D8" modified="2010-06-15" version="1">MASSPSTSDDILDSLKKNVDPTVLSERDAAMRKRVAKQYEKAQNQLRNLVNANSTLPVTISFINIDGAKNTRTGFLRKVLEQTFAESKGPGFTLEDALRELRRDCNTFYKFGIFHPPTLYIDSAGKRDGKTMLKAEISLRELPRFTLKTGTDMGNTEGSGYPEGSGYLSLTYRNMFGGAESLNGYASTGTRTRSAYELNFQAPINANPDLILETSGYASTRSHQYYSSHEEVLRGGKVGVKWGEHETGYTGVWRQITGLAQEASPTIRGDAGDSVKSSLWHAFTRDRRDSHLLPTRGWMVKSESEVAGWGGLGGDVAFWKNEMETQVAAPLGASGVSVSAGLRGGLLYPLCLGVGDNRKPPQPSRINDRFRLGGPTDVRGFKECGLGPRHGNDSVGGDIYMAGGASIYFPLPKVGPEKPLRLQAFVNSGRLVGLQGLAEDSGDVNSSVAGALKELGNGLPSVAAGIGLVYAHPIARFELNFCLPLVKRSGEKGRKGLQLGIGINFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5G895</accession>
    <name>D5G895_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00002938001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430041">
        <property type="protein sequence ID" value="CAZ80738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836547.1">
        <property type="nucleotide sequence ID" value="XM_002836501.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G895"/>
    <dbReference type="GeneID" id="9188340"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002938001"/>
    <dbReference type="HOGENOM" id="CLU_2724040_0_0_1"/>
    <dbReference type="InParanoid" id="D5G895"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ80738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="72" mass="8013" checksum="AEA656D699B29C32" modified="2010-06-15" version="1">MLFLPPPPKQLFENFSIPDVGSPLQVSCCGIIEWTSLESLSRNRSARSHPGWAKKRLGRIICGVIVTSEITF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8J1</accession>
    <name>D5G8J1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00002897001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430046">
        <property type="protein sequence ID" value="CAZ80838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836647.1">
        <property type="nucleotide sequence ID" value="XM_002836601.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8J1"/>
    <dbReference type="GeneID" id="9186058"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002897001"/>
    <dbReference type="HOGENOM" id="CLU_2962571_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8J1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ80838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="59" mass="6854" checksum="C65AB68D33A1065A" modified="2010-06-15" version="1">MSSFQAHARCPTFTTHNNADPTAALFLTTRCSEKPIYQKRREEKMRTKYLEGLALFTFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8M4</accession>
    <name>D5G8M4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00003009001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430047">
        <property type="protein sequence ID" value="CAZ80867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836676.1">
        <property type="nucleotide sequence ID" value="XM_002836630.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8M4"/>
    <dbReference type="STRING" id="656061.D5G8M4"/>
    <dbReference type="GeneID" id="9186235"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003009001"/>
    <dbReference type="eggNOG" id="ENOG502RFKK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016658_1_0_1"/>
    <dbReference type="InParanoid" id="D5G8M4"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004115">
        <property type="term" value="F:3',5'-cyclic-AMP phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047555">
        <property type="term" value="F:3',5'-cyclic-GMP phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006198">
        <property type="term" value="P:cAMP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902660">
        <property type="term" value="P:negative regulation of glucose mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07735">
        <property type="entry name" value="class_II_PDE_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000396">
        <property type="entry name" value="Pdiesterase2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28283">
        <property type="entry name" value="3',5'-CYCLIC-NUCLEOTIDE PHOSPHODIESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28283:SF1">
        <property type="entry name" value="3',5'-CYCLIC-NUCLEOTIDE PHOSPHODIESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02112">
        <property type="entry name" value="PDEase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00388">
        <property type="entry name" value="PDIESTERASE2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="684"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ80867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="684" mass="76167" checksum="C2C31F113321C145" modified="2010-06-15" version="1" fragment="single">KTKIHKVTLSAARRVPGLISLCFFCLSCFGTKPYHKPLPQTTRLFSLQKKVTNLGCLQREAHQQFIYLTHLFFRSDIFAAVPKGERQNRIKRGKNHNLEKTSKSFFFFLKKNSPYPIQIANNCLPYKLAVSSTDDGSPAFQVIVLVSNLRNLVLHPVWICKSFHPTSILTVAVTGYLQGSAGGPSEGNVSGYLIRSTATAWAKGSILAVDAGTHMAGIIRILEEHSSRSSFTKEPIASVSENASGNGSITKRPFPFCGANLPYDTPRGNAAYVTRELVSTYLITHPHLDHLSGFVINTACFTQTECPKRLAALPSTIEAIKTHIFNDIIWPNLSDEECGVGLVTYQRLPEAALEYVEVCSGLSAQAWPVSHGHCMKSHYHRGSQTYQGKDGATADGSPGGSRKRMCVYDSTVYFIRDDATGREVMMWGDVEPDSISLSPRNHPAWNHAAAKFHSGLLNTIFIECSYDCNQPDEALYGHLSPPHLMDEMRSLARLVVSLRRADEVDRRARLKRKRMSYGYHNDDPEIMARRIENPNAPSKILEESQRYHGPGAQEDEVNGIHGNSRWNKNYLEVDSKHSFDRFSPEISPRAVDPKPADFFDTINVAVGQRTPEERAPKGDEGDETFDLGPTPLRGLHLVITHTKDTLLDDVNVPEDILRSIQGLEAVQQLGVTFSLSEQGRSLYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8T1</accession>
    <name>D5G8T1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00004825001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430053">
        <property type="protein sequence ID" value="CAZ80924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836733.1">
        <property type="nucleotide sequence ID" value="XM_002836687.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8T1"/>
    <dbReference type="GeneID" id="9186284"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004825001"/>
    <dbReference type="eggNOG" id="ENOG502SFGZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1195620_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8T1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040233">
        <property type="entry name" value="CCD97-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018613">
        <property type="entry name" value="Ccdc97-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31840">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31840:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09747">
        <property type="entry name" value="CCD97-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCD97-like C-terminal" evidence="2">
        <location>
            <begin position="143"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ80924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="232" mass="26229" checksum="5A3911B364D26705" modified="2010-06-15" version="1">MPGRIVDEMEVDSPALASGPELVDQTINQAAREEDPHSNLDRLLETFLSFLLRACLSRSKGGENEAYLRPCPSQSLNYISKNSSTYFSSPDLELADPLLYDRLIRHYQTPSEREAEGRSKGWSGILEADITRSEAKVEALRENPQETLESRAETNQGQTVSSKEEAEQVWRETMILRFLEGRDEEADYATIDGNEEYDDYRQIERDAQDVYFDAESPSVEGSTIGDTGIQDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9A3</accession>
    <name>D5G9A3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00003222001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430057">
        <property type="protein sequence ID" value="CAZ81096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836905.1">
        <property type="nucleotide sequence ID" value="XM_002836859.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9A3"/>
    <dbReference type="STRING" id="656061.D5G9A3"/>
    <dbReference type="GeneID" id="9182858"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003222001"/>
    <dbReference type="eggNOG" id="ENOG502RZ8N">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015092_4_1_1"/>
    <dbReference type="InParanoid" id="D5G9A3"/>
    <dbReference type="OMA" id="NESTWYW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021514">
        <property type="entry name" value="DUF3176"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35394">
        <property type="entry name" value="DUF3176 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35394:SF5">
        <property type="entry name" value="DUF3176 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11374">
        <property type="entry name" value="DUF3176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="129"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="467"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="558" mass="61800" checksum="9E0FC1822EB57D72" modified="2010-06-15" version="1">MLDPDAKSSPTFPLHRKKRRIDYWAWEWLAILVSFLAIVASTIVLLLFDDRAIPAWSWSSGGISVNAILSLLSTLSRASLLVPIDECMGQLMWLWFSKRERRLADVEVYNQASRGPLGALKLCWRQKGWGLGCLGALLMVCSMGLGVAQQQLLSYPNRSIIQPNSTSTVTRAERFDTYQNVTTSGTTTPEISFSLLSSIYNAALSGSSTGKFSTVNQPFFTCTSGNCEFPPFATLAVCSECFDVSDKVQSSCDADNLCTAEFGGMRAQSWQQPDNNNINTILNQTYLALGSSTMLNSGVASRSSPFSTLAHLQARSENGNWPPKYHGTECALFWCVKTVSAKVHKGVYAEEVISTNTDITNGTNGERSFSLTNGTQVTSFVITPLAQESIQGPKGVTNLLSGWAKGSGYSRVFSSDLMQGFSTQANVTEVMSRVATAMTNRVRDFDGLTPPVNGTTMAKQSFIRVRWPWLAFPVTLWVFSLIFLLTIVVKSREGETKWGMRAWGANSLALMFHGLDEDSRNRVREGEWEEMEDLSERLWVKLERGDEGLKLRARRIDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GA01</accession>
    <name>D5GA01_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00003474001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430068">
        <property type="protein sequence ID" value="CAZ81344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GA01"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003474001"/>
    <dbReference type="HOGENOM" id="CLU_3421428_0_0_1"/>
    <dbReference type="InParanoid" id="D5GA01"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ81344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="24" mass="2775" checksum="3EA75C918DD474E3" modified="2010-06-15" version="1">MTCFQASNAPKKPRGLYYGLWTNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAD3</accession>
    <name>D5GAD3_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">rRNA 2'-O-methyltransferase fibrillarin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Histone-glutamine methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00005244001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-glutaminyl-[histone H2A] + S-adenosyl-L-methionine = N(5)-methyl-L-glutaminyl-[histone H2A] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50904"/>
            <dbReference type="Rhea" id="RHEA-COMP:12837"/>
            <dbReference type="Rhea" id="RHEA-COMP:12839"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30011"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61891"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. Fibrillarin family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430075">
        <property type="protein sequence ID" value="CAZ81487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837296.1">
        <property type="nucleotide sequence ID" value="XM_002837250.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAD3"/>
    <dbReference type="STRING" id="656061.D5GAD3"/>
    <dbReference type="GeneID" id="9182522"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005244001"/>
    <dbReference type="eggNOG" id="KOG1596">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165767_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAD3"/>
    <dbReference type="OMA" id="SYERDHA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031428">
        <property type="term" value="C:box C/D methylation guide snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990259">
        <property type="term" value="F:histone H2AQ104 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008649">
        <property type="term" value="F:rRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000494">
        <property type="term" value="P:box C/D sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000692">
        <property type="entry name" value="Fibrillarin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003276">
        <property type="entry name" value="PRK04266.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10335:SF17">
        <property type="entry name" value="FIBRILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10335">
        <property type="entry name" value="RRNA 2-O-METHYLTRANSFERASE FIBRILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01269">
        <property type="entry name" value="Fibrillarin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00052">
        <property type="entry name" value="FIBRILLARIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01206">
        <property type="entry name" value="Fibrillarin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ81487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="116" mass="13135" checksum="0FEA46AEAD571E74" modified="2010-06-15" version="1">MAKKRTNVIPIIEDARHPLKYRMLVGMVDVVFADVAQPDQARIVGLNCHLFLKEKGGVLVSVKANCIDSTAAAEAVFAREVQKMREERIKPLEQLTLEPFERDHCMVVGRYLRSQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAK2</accession>
    <name>D5GAK2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="9">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00003621001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. SKI2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="9"/>
    <dbReference type="EMBL" id="FN430078">
        <property type="protein sequence ID" value="CAZ81545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837354.1">
        <property type="nucleotide sequence ID" value="XM_002837308.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5GAK2">
        <property type="interactions" value="372"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GAK2"/>
    <dbReference type="GeneID" id="9187485"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003621001"/>
    <dbReference type="eggNOG" id="KOG0952">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_247058_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAK2"/>
    <dbReference type="OMA" id="VYGYQSH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001076">
        <property type="entry name" value="ATP-dependent DNA helicase MER3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.10:FF:000012">
        <property type="entry name" value="DEAD/DEAH box DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000012">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.10">
        <property type="entry name" value="Sec63 N-terminal domain-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052247">
        <property type="entry name" value="Meiotic_Crossover_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004179">
        <property type="entry name" value="Sec63-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057842">
        <property type="entry name" value="WH_MER3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47835:SF3">
        <property type="entry name" value="HELICASE FOR MEIOSIS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47835">
        <property type="entry name" value="HFM1, ATP DEPENDENT DNA HELICASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02889">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23445">
        <property type="entry name" value="WHD_SNRNP200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00973">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158702">
        <property type="entry name" value="Sec63 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-0469">Meiosis</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="249"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="459"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="151"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1120"/>
            <end position="1146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1189"/>
            <end position="1243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="151"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="161"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ81545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1535" mass="170243" checksum="FE40355E3081BE66" modified="2010-06-15" version="1">MEDLFDELMGVLGDEAPRDSTGREYDAGPSRQESGFSFDVSHQPREYSRYWPMQSWEDLSRYTASQQLVDTGRNRVQMRPRDAIEQASALGLGLGGVPSRGYNTEYPLQGGVVGSYRDGGRRNPTWENVGLSGTLQSPHFGHSVGISSTAEATWEIPSSQPRRRATSRGPRRLLGIRGVGDTTESRFRPPLRAAPAAKVSSAPSGTISHSHAPPMVQGVSLLSVNILPDKHRGLFQFPLFNAVQSKCFPLVYESDDNVVLSSPTGSGKTAILELAICRLSVKMQPGSYKIVYQAPTKALCSERKQDWDKRFSVIGLKCTELTGDTQQSQLRNVKDGDIIVTTPEKWDSMTRRWEDHRKLLEMVRLFLIDEVHILKEDRGATLEVVVSRMKSIGSDVRFIALSATVPNSADIAEWLGKCSSVSQSPAHDQRFGEEFRPVKLQKFVYGYQSPGNDFVFDKKLDRALPEVIRKHSAGKPIMVFCMTRAMCISTAKVLAQCCSTVKPADRMWPAPTIQFSFKDKDLHATGVCGVAFHHAGLDQNDRALVEKLFLEGHLSVICCTSTLAVGVNLPTHLVVIKNTVTWSNGGPKEYVDLEVMQMLGRAGRPQFDDTGAAVLMTRREHRVRYEKMVSGTETVESCLHENLVEHINAEIGLGTITNAESAKRWLRSTFLYVRLKKDPSHYGITGKGGIQDIEERLEEICERNIKLLTEEGLVLTGGGRLRCSELGLAMARYYVKFGSMKTILHLQERAGLSDVLVALSSAEEFKEIRFRSGEKGLYKELNKSPGMRFPVKGDLWNSSHKVQLVIQFEIGMMEFPPEAGFQKYKTSLLQDKALVFQHCHRIIRCIIDCRICSKDATTVKAALELGRSLKAKAWEDSPSQLRQIEGFGPVAIKKLVNAGIRNLEVLARLESHKVETALSRNPPFGANVLKIAQSVPLFRVFCSQISASGNGRDPVKLVVKAELGFLNSHPPERFRGNLLFANILAERSDGLLVDFRRIPVPKLDGGKTIQFKVDLETPEQGVLCHIVCEDIVGTIKTHVLKPVVNIRLFPRNAVISPPKNPHGHAIDSSAVIEHPGGEEEYGDAEFIAAVEDLDFSHIAGLGESLTNQTRLNTAANARMPASNANEVKESAASEEAGQEAHQLPNGNWECKHKCGDKTRCKHFCCREGLEKRPKAIKVTKDTVAIKAGTIPGAKPAERGSRGPENNGLQGLPYHRQANSVSKGVRSDEDFASSLPPPKPNLPLSTEITRLKRLHEAHGGETRSKVRRISAMPFPQGKHTGQSVSRKIHGERLSDDDDGLPTLERLLREGTGYKWCPEATASEPRKAILRHPIGKKPDAKKDGVNGVQRIESRAEPVGMEQIPAVHASHGKGRLVLNTKPGDEIIDSKERILYPPISSLNKSLDEPVGEGDNIQKVEHRTKREDTVLYEDMKACDVKQEHEVAVVPPTGLQVGGIKQEDYEPIDLSVPVPAEGLKYESETEYSRGQRPKTEHNEDLDYIVMYGSQGSIKKDCEVKWEETEETLQEKDLFEDFVIYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAQ2</accession>
    <name>D5GAQ2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00003728001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430084">
        <property type="protein sequence ID" value="CAZ81595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837404.1">
        <property type="nucleotide sequence ID" value="XM_002837358.1"/>
    </dbReference>
    <dbReference type="GeneID" id="9187761"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003728001"/>
    <dbReference type="eggNOG" id="ENOG502QVRM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_254556_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAQ2"/>
    <dbReference type="OMA" id="VAHANAY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058925">
        <property type="entry name" value="zf-C2H2_AcuF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35391">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35391:SF3">
        <property type="entry name" value="FINGER DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_8G04300)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26082">
        <property type="entry name" value="zf-C2H2_AcuF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="999"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="338"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="538"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="725"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="858"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1119"/>
            <end position="1289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="92"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="216"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="253"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="338"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="429"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="538"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="579"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="606"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="772"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1142"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1221"/>
            <end position="1241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1250"/>
            <end position="1265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1279"/>
            <end position="1289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ81595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1397" mass="151882" checksum="9756A2EBE09FCE6B" modified="2010-06-15" version="1">MASPLYPQHSRAPPVITEDDGSNSRNVYPTPNSAHGPGTFDTSLFSLSGSGNTHLSERPQPALPSLQSSTYPHTNSPLLPTINQPLSNQGHSPSLTSGSSGLSPATSQHSYYTHNIGLTCSEDPSSALSPPVSFVVDPGSSPERGLGPGYSVDTTPGFDEVDMPPLADENTALYGDEGIDPLDQLSDPYSPVNGSLGIYHPPSPRPTVSPSPAGLTASNPGGFTGVNSNFRKSPVVKVEEFTPQDNGVHPTPSSRAMTGSSSSSGAGAAQGGDNLTVNRGEDGSWKGGLDPTARREVELGLGDLPFSLKEQEIIRGIEEKNADIEDWLSRNTNLSIKPTDKRRSRSLADFRRRPMVPPKSKDDCVRPGESSSTGAVLFDEDDDTGLPGAYDYEDDDSSVASSFVDGSLDDSPEDQGSMDTSEIPPSESELMKTAEEEEAEQKRRENDPQYYPRPNQFYSAHPWNDTVAQVGRGAAIASRNQPNTANAAITKFYRYAENIETASRVATFGSQMSKSRRLSAGDADRFLANGLLKSLSFGRDKDKEKEREKEKEKQLNHQRRPSMWGTIPSRLKRGFSNAGDKEREKEKEKERLGTGLSMEGRKRGNSTTSLASTASHAFAPIKRGSSWAKSAPKSPLKVDTKNIGGAFAQMASMAAGAGQSSAHTAHTASTAGIISPPTRSLSGGFIAQTVKRARSKSDISSSKLQKGKPVFGIVSMLGQYGGPPALPIKSPVQSAESESPRRNYTFGEESRKRGIAAAKLLCPPDPHRHDGYDDDDDNDMDDDDMQNSQQTTPPGTRPPLPKLDITPNPEGFSQHVRGLTPALHPKLVDRVVYEQGKRFKKLVEHRQKHLAAIRGGKRCGNTTKCRGPVGSIGPGGDPSANGHKRNTSGGSGEGEAYSSDETVGDGSRSPTEGKVTATQFPPGVPQPPVLRLPAEFECPICFKVKKFQKPSDWTKHVHEDVQPFTCTFVECTEPKSFKRKADWVRHENERHRHLEWWTCTLPDCTHTCYRKDNFVQHLVREHKMAEPKVKAQKAAARKGKKGVAGMSEGVPSHEDFEKVWQIVDSCHQVTTKHPTQEKCRFCGNVCPTWKKLTVHLARHMEQISLPVLDLIKDDAVVPPSVRPSAASKRVQQNSDSSKPEPSKPPPPPPPPPIQQPDMEMDIDPADTTQRQLTGNGDCDVLPPPQFHSPDASTFDMSPAHLQYTPPRSSPNVDDRFGIQTPLRTPQFVSPHNFSPEQNLRMQQQQQQQQLSMGTNSGFDFSNNTGRDIYSLSPQQQQQHTPTSSGVTTPQRIATQAGGYINVSPPPQPGLQYPQHRSPLIHLVGQQQMAQQPQYFAYGHASGQVQGAHGQIHSQTVAHANAYNFYPQLEQNDLEIPGAAVTAADPTDDYINVAYTAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>D5GB72</accession>
    <name>D5GB72_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="12">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00003773001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430087">
        <property type="protein sequence ID" value="CAZ81765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837574.1">
        <property type="nucleotide sequence ID" value="XM_002837528.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GB72"/>
    <dbReference type="GeneID" id="9181475"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003773001"/>
    <dbReference type="eggNOG" id="KOG0762">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015166_16_1_1"/>
    <dbReference type="InParanoid" id="D5GB72"/>
    <dbReference type="OMA" id="WVTATPF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF10">
        <property type="entry name" value="SLC (SOLUTE CARRIER) HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="92"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="191"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ81765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="273" mass="29003" checksum="628D5C2D8DBB5A39" modified="2010-06-15" version="1">MADFYAGYISGAIGILLGSPLDILKVHLQSRSTTTTTTSSSSPSLFPTATSYVRGTAAPLLGYGCLNAILFHTYNRTLSLLSTPQQQQQQQSSLAMIWTAGAVSGAVSFVLSAPVELVKVRAQTRPGENSYEVARGVVKREGVRGLYLGGGVTGVRDSAGYGFYFWAYEYMKRLLRYEEAAGGGGGEGQRAASMKTLLAGGVAGCVSWLSVYPLDVIKTRVQAQGEREGLLGNRRLGTWECAKSAYAGGGGRVFFNGLGVCMARAFLVRPLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5GB84</accession>
    <name>D5GB84_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Vacuolar protein sorting-associated protein 74</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00003785001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Phosphatidylinositol-4-phosphate-binding protein that links Golgi membranes to the cytoskeleton and may participate in the tensile force required for vesicle budding from the Golgi. Thereby, may play a role in Golgi membrane trafficking and could indirectly give its flattened shape to the Golgi apparatus. May also bind to the coatomer to regulate Golgi membrane trafficking. May play a role in anterograde transport from the Golgi to the plasma membrane and regulate secretion. Mediates the cis and medial Golgi localization of mannosyltransferases through direct binding of their cytosolic domains. Involved in vacuolar protein sorting.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">Golgi stack membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GOLPH3/VPS74 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430087">
        <property type="protein sequence ID" value="CAZ81777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837586.1">
        <property type="nucleotide sequence ID" value="XM_002837540.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GB84"/>
    <dbReference type="FunCoup" id="D5GB84">
        <property type="interactions" value="511"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GB84"/>
    <dbReference type="GeneID" id="9181487"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003785001"/>
    <dbReference type="eggNOG" id="KOG3983">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036311_1_1_1"/>
    <dbReference type="InParanoid" id="D5GB84"/>
    <dbReference type="OMA" id="GETWNLL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005797">
        <property type="term" value="C:Golgi medial cisterna"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019899">
        <property type="term" value="F:enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070273">
        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048194">
        <property type="term" value="P:Golgi vesicle budding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035269">
        <property type="term" value="P:protein O-linked glycosylation via mannose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045053">
        <property type="term" value="P:protein retention in Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060304">
        <property type="term" value="P:regulation of phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3630.10:FF:000002">
        <property type="entry name" value="Vacuolar sorting-associated 74 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3630.10">
        <property type="entry name" value="yeast vps74-n-term truncation variant domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008628">
        <property type="entry name" value="GPP34-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038261">
        <property type="entry name" value="GPP34-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12704:SF2">
        <property type="entry name" value="GOLGI PHOSPHOPROTEIN 3 HOMOLOG SAURON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12704">
        <property type="entry name" value="TRANS-GOLGI PROTEIN GMX33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05719">
        <property type="entry name" value="GPP34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00058727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00073084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ81777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="350" mass="38922" checksum="680CA6806E0D45A4" modified="2010-06-15" version="1">MASNTGLTRRRGGGGGGGSAGDEVDRSSSPSTSRPAEPRSGETSFTTAENGHKIAFDPRDISENQERSKQPKLTLMEEILLLGLKDKQGYLSFWNDNISYALRGCIVLELAFRGRVSMQKDSNRRRFPLSDRIIEVIDDTLTGEVLLDEALKMMKASEKMSVSSWIDLMSGETWNLMKIGYQLKQVRERLAKGLVDKGILRTEKRNFLLFDMATHPVADGGAKDEIRRRVRNVLTSRTVVLPGNPFLPEDLEFRYLRTVAMVCAAYAANVLENALATLGHEARERAFAHVDELLAEFSQWPFAKRNTAGGVGQNLGTATTEELNKNKDRELQIEVVAAVLSVFTRLDSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBS7</accession>
    <name>D5GBS7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00005562001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ81927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837736.1">
        <property type="nucleotide sequence ID" value="XM_002837690.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBS7"/>
    <dbReference type="STRING" id="656061.D5GBS7"/>
    <dbReference type="GeneID" id="9182964"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005562001"/>
    <dbReference type="HOGENOM" id="CLU_1031312_0_0_1"/>
    <dbReference type="InParanoid" id="D5GBS7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41677:SF1">
        <property type="entry name" value="FE2OG DIOXYGENASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41677">
        <property type="entry name" value="YALI0B19030P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ81927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="270" mass="30067" checksum="0EB075869AB2D2D7" modified="2010-06-15" version="1">MVGGETVIQRGDGTFFGISQPGTGSAAVLQGGSLKHLALMAKNSPDRITLVTSYRAKAVGLWDISFLTNVRPYTDLSVLYPQWSAYRLRVLSENTAAMTRRLASSSVPRAELETFIRRQQEYLRITTEQIVTEPTVSSTIAQVGINGFYKVLGMYLSNSIFANAPSVCPQCGNVGKVDKRHLAECVRMREWRPEADVWIVFEDSLKEMSAGGAVVVEKTTRPDLEEVAKVFQKDFQAGRRNSWGIADELARLGLTEYLLEYLRFFGIVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBW2</accession>
    <name>D5GBW2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00005614001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ81962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837771.1">
        <property type="nucleotide sequence ID" value="XM_002837725.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBW2"/>
    <dbReference type="STRING" id="656061.D5GBW2"/>
    <dbReference type="GeneID" id="9185397"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005614001"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_30_13_1"/>
    <dbReference type="InParanoid" id="D5GBW2"/>
    <dbReference type="OMA" id="VYIAWIC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000217">
        <property type="entry name" value="MFS lactose permease, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF70">
        <property type="entry name" value="MONOSACCHARIDE TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G14540)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="48"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="152"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="182"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="217"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="304"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="346"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="370"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="398"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="440"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="466"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="53"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ81962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="527" mass="58226" checksum="C2851279C638BA4F" modified="2010-06-15" version="1">MGSGETIFSDIENFEGDSNDSGKNAIAPSNIDAIVQENKSSLLSRNSLKLYFCLLVSYLVSTMNGYDGSLMGAVNDMEQYQRSFGLNGKGSSTGIVFIIYNLGQIAAFPAIGWLADGYGRRWGMFVGCAIVVVGTAVQSTANGLGQFQGGRFILGFGAAIACASAPAYAVELSHPKYRGTQAGLYNVCWYVGSIIAGWCCVGSNRHMNNSWAWRTPTVVQGAIPAIVAILIFFIPESPRWLIAQGRDEEAIEILAKYHGEGDRHSSVVQAEFREMSEQIGTECSDKIWWDYRDLVNTRAARYRLGLVATVAFFGQWSGNNVVSYYMPTMFQQTGIDSSDTRLVLNGIYPVFCMFAAIWGATLLDKLGRRPMLIVATAFTVVCFSIITAGTAVSSTSKGASYAVIAFIYIFGMVYSWAYTPLQTLYSTEVLETRTRAKGSGLNFLFVNIAMCVNIFAAPVAMEAIGWRYYIVFIGWNCFEVFVIWMWFVETAGHTLEQISEIFESRNPVKMSLETRKRNRVRNSINNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5GC51</accession>
    <name>D5GC51_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00000566001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430100">
        <property type="protein sequence ID" value="CAZ82094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837903.1">
        <property type="nucleotide sequence ID" value="XM_002837857.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GC51"/>
    <dbReference type="STRING" id="656061.D5GC51"/>
    <dbReference type="GeneID" id="9181363"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000566001"/>
    <dbReference type="HOGENOM" id="CLU_1138710_0_0_1"/>
    <dbReference type="InParanoid" id="D5GC51"/>
    <dbReference type="OMA" id="IRWIMWR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140042">
        <property type="term" value="P:lipid droplet formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23995">
        <property type="entry name" value="Seipin_BSCL2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009617">
        <property type="entry name" value="Seipin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21212">
        <property type="entry name" value="BERNARDINELLI-SEIP CONGENITAL LIPODYSTROPHY 2 HOMOLOG BSCL2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21212:SF0">
        <property type="entry name" value="SEIPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06775">
        <property type="entry name" value="Seipin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="116"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="157"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="162"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="189"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ82094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="244" mass="26502" checksum="17AFCAF178134968" modified="2010-06-15" version="1">MAEITIYSTPLSPSSSASMPGMVIATGRRGGLMPWRSPIARTAHTLLRLPLLLFLPNAKEEEAIRVVLLENVVFGELARHAVVSLESRSRLDVYEAKVEFRAKLAGIRWIMWRWKVLSFFLGSGLFFAVEVLAMGVVWWAVYRWFTSSGKLAASGGGRYLEGEDDTVEEEEDDGVDGVDDLGFATPESLEADDEEDDDEVLLKSDVGDSGIGGLSESLGTTAREKSAGGASGSGGSRRRRTRLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCM2</accession>
    <name>D5GCM2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00000725001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430114">
        <property type="protein sequence ID" value="CAZ82265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838074.1">
        <property type="nucleotide sequence ID" value="XM_002838028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCM2"/>
    <dbReference type="STRING" id="656061.D5GCM2"/>
    <dbReference type="GeneID" id="9187884"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000725001"/>
    <dbReference type="eggNOG" id="KOG1337">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041939_3_2_1"/>
    <dbReference type="InParanoid" id="D5GCM2"/>
    <dbReference type="OMA" id="VWDEVCI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19177">
        <property type="entry name" value="SET_SETD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1410.10">
        <property type="entry name" value="set domain protein methyltransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050600">
        <property type="entry name" value="SETD3_SETD6_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044429">
        <property type="entry name" value="SETD4_SET"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271:SF137">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271">
        <property type="entry name" value="UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SET" evidence="1">
        <location>
            <begin position="90"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="440" mass="50309" checksum="1AD91BBA3BCA7B51" modified="2010-06-15" version="1">MTDRKTTPTPAVTGTHALFTLWARKHGIEFPKLIPAYLPPAGLGIISTDEIAGPDPIRHGRTNDTSLGEVIAFTPNALLLTRENIERFSPGCGLRSFLRQKVDDGGLSSHAVFATAIAREIRRIAEGHENMWREWIDVWPKAKEFRGSMPLMWSAEEGDLLPPSTKRLLDEQRGKFKRDFKAVVQKGFEFTKQEFMWAWIIVNTRTLFYKPAHSAYNDLPREHCMALCPFIDYFNHSDDGCKVQLTPTGFTVTPTKPSHPANTQLFVTYGSHSNDFLLVEYGFNLPLEKNKWDEVSITPTLLPLLSEEHKRVLSREGFLGNYAFDKEAFCFRTQAAVRLMLIEHPEMDSNWRKVEVWKNFLAGNDDGERERYAVEEKLLGLLDSLEADGERALLEADKLQRQSIRDVIKMRWEQILGMTNDVRANMVGETGKWRGRSHVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDR9</accession>
    <name>D5GDR9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="15">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">GSTUM_00006243001</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Nucleoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the non-repetitive/WGA-negative nucleoporin family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430153">
        <property type="protein sequence ID" value="CAZ82662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838471.1">
        <property type="nucleotide sequence ID" value="XM_002838425.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5GDR9">
        <property type="interactions" value="1156"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GDR9"/>
    <dbReference type="GeneID" id="9181667"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006243001"/>
    <dbReference type="eggNOG" id="KOG1900">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000429_0_1_1"/>
    <dbReference type="InParanoid" id="D5GDR9"/>
    <dbReference type="OMA" id="SWAPFQK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044611">
        <property type="term" value="C:nuclear pore inner ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051292">
        <property type="term" value="P:nuclear pore complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036228">
        <property type="term" value="P:protein localization to nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006405">
        <property type="term" value="P:RNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000972">
        <property type="term" value="P:transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.440:FF:000001">
        <property type="entry name" value="Nuclear pore complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.450:FF:000002">
        <property type="entry name" value="Putative non-repetitive nucleoporin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1880">
        <property type="entry name" value="Nucleoporin, helical C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.440">
        <property type="entry name" value="Nucleoporin, helical domain, central subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.450">
        <property type="entry name" value="Nucleoporin, helical domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007187">
        <property type="entry name" value="Nucleoporin_Nup133/Nup155_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014908">
        <property type="entry name" value="Nucleoporin_Nup133/Nup155_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004870">
        <property type="entry name" value="Nucleoporin_Nup155"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042533">
        <property type="entry name" value="Nucleoporin_Nup155_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042537">
        <property type="entry name" value="Nucleoporin_Nup155_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042538">
        <property type="entry name" value="Nucleoporin_Nup155_C_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10350">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10350:SF6">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03177">
        <property type="entry name" value="Nucleoporin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08801">
        <property type="entry name" value="Nucleoporin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="9" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nucleoporin Nup133/Nup155-like N-terminal" evidence="14">
        <location>
            <begin position="128"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin Nup133/Nup155-like C-terminal" evidence="13">
        <location>
            <begin position="653"/>
            <end position="1301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="7"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="29"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF03177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAZ82662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1339" mass="148628" checksum="BD342BC9FAE16A56" modified="2010-06-15" version="1">MSIFETPPSQRQYHSSLNPVYPDLPVELRSSSRNSYTGGSSSAYVADGRSSQPFAAVPSGSESGVPQAPKPVSSTDNLGPVPRAARTINEFLTTEARYPQLDDIVSQGQSSDYDLPRSSAWEPFQRTALHTIPDSIFEQYNRATSHTMMGLFAELQQAWITVDNRLYLWDYTTQGSFQGYEAQPNNITAVRLLSPKPGVFVGDVNYVLVIATTLDIFLLGVQAVPNARGGRDVTLFETKMSVPTKGLGISVIEGSKKTGRIFFGGRGDNDIYEFTYQAEEKWFYGRCGKICQTSGGVSSFAPRIPLPFLSGPKDTEYIVQMVMDDTRNLLYTLSSKSAIRAFHVKGETAFSLSITHSFSHTLANIRVMIGNTPLLEPKTPIVSISPVTSQQARRTHLIATTTTGCRLYMSAVASEYGFGGSESAPTSMQVIHVRFPPSTGPTLTPTRKAKVFAPGYFFCFVDKPEAVDELFLSAPDSGRIAILAEGTSRATLIETGMSVNLDSRVEAVELISVPFAASKGPNGFGNESAVQYDLPPTEVAILTNSGVHIIKRRRLIEVFDAAIRYGMTSSPIGVEGEARNFFEKYGRAEGCASSLAVACGASTETVNQSQARTTDVEVSEFARKFFIDYGGRPRAENVYDASALPSLDSVKVSGRAEGLALYISRIVRSIWKAQMVVEVPSVAGVASYHSGVPVEKLLSIQEQLVKLSAFLQNNRSFINGLSGAESLMGLGSRVEEVAQQAEHRMLHALVTLVGNMIEGISFVLFMLDDKLGDIILSLPDNQKEEAKRVTYAGLFTSDSGAALAKELVTAIVNRNIAAGLNVDTIADALRRRCGSFCSADDVVVFKAIEQVRRAKTDGDPDNRNRLLKESLRLFEETAASLTMENLRDTLIEYRSLNYYPGSVQLALTVARESDRGNEALGFLADGAVPTDPRAHFHAKREECYRLVFEVLDELDSKFGQSPEMQDGMPTPATRLRNETWQLVYTSDDEVFHNTLYDWLHRRGLSDRLLEVDSPYVLGYLQRRSSESLDHYDLLWQYHARRESFYGAAEVLHKLAQSDFDLSLGKRLEFLSRAKGFCGSYGPMGIRQKMNELSHTLQEELDVAVIQDDVLRRVKEDGRINSSKKEKLIKELDSNLLTLSDLFNRFADPYGYMDICLAIFQGADYRGAGEIKKCWEQLISSIHTDAEASGQGHPYELVADNIRRLGHRFNNSEYIFPPNDLVQLLEIYAYEHQRDVGAPTWVVDCLLEAGIPAETILRILDEMYWRDEVPFQGTVRRRLVRDMAYVAEKWFTHAVRRGGEALAKKEFVKGVLTGLSRVTSDNDDRLRIMGILEEIRRRMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDW5</accession>
    <name>D5GDW5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8">Eukaryotic translation initiation factor 2A</fullName>
            <shortName evidence="8">eIF-2A</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00006311001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40S subunits in a GTP-dependent manner.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the WD repeat EIF2A family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430153">
        <property type="protein sequence ID" value="CAZ82708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838517.1">
        <property type="nucleotide sequence ID" value="XM_002838471.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDW5"/>
    <dbReference type="FunCoup" id="D5GDW5">
        <property type="interactions" value="1145"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GDW5"/>
    <dbReference type="GeneID" id="9184503"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006311001"/>
    <dbReference type="eggNOG" id="KOG2315">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013809_0_1_1"/>
    <dbReference type="InParanoid" id="D5GDW5"/>
    <dbReference type="OMA" id="RCCAYSP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000596">
        <property type="entry name" value="Eukaryotic translation initiation factor 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011387">
        <property type="entry name" value="TIF2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013979">
        <property type="entry name" value="TIF_beta_prop-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13227">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13227:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08662">
        <property type="entry name" value="eIF2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017222">
        <property type="entry name" value="eIF2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="7 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 8" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Translation initiation factor beta propellor-like" evidence="10">
        <location>
            <begin position="212"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="472"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="531"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="582"/>
            <end position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR017222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ82708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="713" mass="78251" checksum="EE1CA1DED0179072" modified="2010-06-15" version="1">MAATGVTTQFAYRTQKTIGLIDGHPQYSHQPGFNKPETNNRCCTYSPDGRYFSWACNEDVKIANPNTGEIVTTLPAQNVHEIGFSPRGSYIITWERPTKQEDGSASKNLRVWKTETAEHVVAFVQKSQSGWNLQYTYDEKYCARVVTNTVQFFESTNMNEVWKILRVEGVTEVALSPGKNYSVAAFVPERKGNPAVVKIFQVPNFDDVLSQKSFYKADRVQLKWNALGTSVLVFSQTDADKTGKSYYGETNLYLITVAGNYDCRVSLDKEGPIHDVAWSTDSKEFGVVYGFMPAKTTIFDARANIIHQLPAGPRNTILFSPHARLVLVAGFGNLQGSMDIYDRQAGMKKICTIEASNASVCEWSPDGRHILTATTSPRLRVDNGVKIWHYTGGLMYNIDLDELYHVTWLPQSASMHPLPSPLPPAPTPHESALTHVATTTPKKPAGAYRPPHARNTATPLHFKREDEGGAAHIYANGSGAGSGSGSNGLFSGKGRRREVPGAAPAEKLVPGAAPGGGVSLAGTGAGADEENLSRAAVKNKKKREAAKKAKAEQQQAANAAGNGIPQGPQPDREGREGNGRNPHQHHTRSKSRNNQQNEGHRRSKSKTGGENQNHHGRHHSEHQQLHIRTGADSLAPPIDPSTPQKRQTDLEAEPQTPDGTPSTQDKKLRGLHKKLRAIEDLKQRLANGEKLEDTQLRKIRTEEQVRKELNGLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>D5GE00</accession>
    <name>D5GE00_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="12">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00001155001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the USE1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430157">
        <property type="protein sequence ID" value="CAZ82743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838552.1">
        <property type="nucleotide sequence ID" value="XM_002838506.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GE00"/>
    <dbReference type="STRING" id="656061.D5GE00"/>
    <dbReference type="GeneID" id="9183075"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001155001"/>
    <dbReference type="eggNOG" id="ENOG502SCD1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027976_0_0_1"/>
    <dbReference type="InParanoid" id="D5GE00"/>
    <dbReference type="OMA" id="YAWIFGL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019150">
        <property type="entry name" value="Vesicle_transport_protein_Use1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13050">
        <property type="entry name" value="USE1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13050:SF7">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN USE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09753">
        <property type="entry name" value="Use1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="265"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="97"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="146"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="189"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ82743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="295" mass="32516" checksum="154A69A146DE40A1" modified="2010-06-15" version="1">MALPIAATESTITTKNLTRLLSRLDSKLASRKAAPLSTVDRAKFAANLEYARQLLLSLESEPSLIRNASTRQSVLSGLGVQKQAIRRLNEQLLVMAAEGEEEGGGSTDDDSDGEEEEEVKEVSALPAQVNGGERPQQSESIPAPATTTTSTLRNRFQQPTTTPTNHHQREQLFPPRPDPTAAATDTNTDTEKHLDHQRAEQEHITADMLSLAQALKQSSLRFGSELENEKVLLDMAREGLDKNALGIEGTGMKLKALRQDESVGFLWSILYPVIIVGLAFMVLFVLFFAPKLRWW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>D5GE11</accession>
    <name>D5GE11_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00001147001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the membrane magnesium transporter (TC 1.A.67) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430160">
        <property type="protein sequence ID" value="CAZ82754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838563.1">
        <property type="nucleotide sequence ID" value="XM_002838517.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GE11"/>
    <dbReference type="STRING" id="656061.D5GE11"/>
    <dbReference type="GeneID" id="9183087"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001147001"/>
    <dbReference type="eggNOG" id="ENOG502S8V0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_132206_0_1_1"/>
    <dbReference type="InParanoid" id="D5GE11"/>
    <dbReference type="OMA" id="YGFWDIR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053279">
        <property type="entry name" value="EMC_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018937">
        <property type="entry name" value="MMgT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28144">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28144:SF1">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10270">
        <property type="entry name" value="MMgT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072053" evidence="6">
        <location>
            <begin position="21"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ82754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="113" mass="12592" checksum="2FDACD708C6C0F21" modified="2010-06-15" version="1">MPSRVLISLSALLLIHSCYSAHEHSLLMPTTSLPLDVAVEMLVSVVLLCFGIVLGNREELKPISWSVWSGLLEREKGYGQFSYLEERVGFLDIRAKRAEFAKWVKGAEEGSSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>D5GE91</accession>
    <name>D5GE91_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00001228001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430168">
        <property type="protein sequence ID" value="CAZ82834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838643.1">
        <property type="nucleotide sequence ID" value="XM_002838597.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GE91"/>
    <dbReference type="GeneID" id="9185498"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001228001"/>
    <dbReference type="HOGENOM" id="CLU_2016890_0_0_1"/>
    <dbReference type="InParanoid" id="D5GE91"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="123" mass="13098" checksum="E112FF9011987301" modified="2010-06-15" version="1">MGIRSSHSLVSTYPIGTRSPEPPPLDPPLLCISTESMDGISKVISSSAPNSADKPRYFRRFPFLHNPPVKGTGAVVITSPGCMGSGINPVLVTGQLATSKLGFFLLGLFVVRSHKDCPLVYNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5GE99</accession>
    <name>D5GE99_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">AA9 family lytic polysaccharide monooxygenase</fullName>
            <ecNumber evidence="15">1.14.99.56</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Endo-beta-1,4-glucanase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Glycosyl hydrolase 61 family protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">GSTUM_00001211001</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Lytic polysaccharide monooxygenase (LMPO) that depolymerizes crystalline and amorphous polysaccharides via the oxidation of scissile alpha- or beta-(1-4)-glycosidic bonds, yielding C1 and/or C4 oxidation products. Catalysis by LPMOs requires the reduction of the active-site copper from Cu(II) to Cu(I) by a reducing agent and H(2)O(2) or O(2) as a cosubstrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>[(1-&gt;4)-beta-D-glucosyl]n+m + reduced acceptor + O2 = 4-dehydro-beta-D-glucosyl-[(1-&gt;4)-beta-D-glucosyl]n-1 + [(1-&gt;4)-beta-D-glucosyl]m + acceptor + H2O.</text>
            <dbReference type="EC" id="1.14.99.56"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 15">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="15">Has a modular structure: an endo-beta-1,4-glucanase catalytic module at the N-terminus, a linker rich in serines and threonines, and a C-terminal carbohydrate-binding module (CBM).</text>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the polysaccharide monooxygenase AA9 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.99.56" evidence="15"/>
    <dbReference type="EMBL" id="FN430169">
        <property type="protein sequence ID" value="CAZ82842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838651.1">
        <property type="nucleotide sequence ID" value="XM_002838605.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GE99"/>
    <dbReference type="STRING" id="656061.D5GE99"/>
    <dbReference type="GeneID" id="9185501"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001211001"/>
    <dbReference type="eggNOG" id="ENOG502RXMI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031730_0_0_1"/>
    <dbReference type="InParanoid" id="D5GE99"/>
    <dbReference type="OMA" id="NAHAIFQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008810">
        <property type="term" value="F:cellulase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030248">
        <property type="term" value="F:cellulose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd21175">
        <property type="entry name" value="LPMO_AA9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.50.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049892">
        <property type="entry name" value="AA9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005103">
        <property type="entry name" value="AA9_LPMO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000254">
        <property type="entry name" value="CBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035971">
        <property type="entry name" value="CBD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33353:SF17">
        <property type="entry name" value="ENDO-BETA-1,4-GLUCANASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33353">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_1G12560)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03443">
        <property type="entry name" value="AA9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00734">
        <property type="entry name" value="CBM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00236">
        <property type="entry name" value="fCBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57180">
        <property type="entry name" value="Cellulose-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51164">
        <property type="entry name" value="CBM1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 15" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6 15" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="8" id="KW-0186">Copper</keyword>
    <keyword evidence="10 15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12 15" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 15" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="CBM1" evidence="16">
        <location>
            <begin position="219"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU368122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAZ82842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="255" mass="25792" checksum="B0F869B33CD514BC" modified="2010-06-15" version="1">MADNSAVVGTTSLIPPTRDPLLSTLLPASSEGAGSVWTKIAESGLSGGKWAVETLIANGGKHHVTLPTALAAGDYLLRAEIIALHEAETDYPVNPARGAQFYPSCSQFKVTGSGTVTPPGTFGFVGGYSHNDPGILFNLYNGATSYTIPGPAVWDGKTGGSAPAPSSTAVVPSTTRSVTTALAATTAPAATSVSAPGRTSSAIVPAASSPTKPAGDNSGIVAKYYQCGGKIHTGGTNCVAGTKCTFQNEYYSYCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEC9</accession>
    <name>D5GEC9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00001270001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430174">
        <property type="protein sequence ID" value="CAZ82872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838681.1">
        <property type="nucleotide sequence ID" value="XM_002838635.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEC9"/>
    <dbReference type="GeneID" id="9185528"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001270001"/>
    <dbReference type="HOGENOM" id="CLU_2484960_0_0_1"/>
    <dbReference type="InParanoid" id="D5GEC9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="87" mass="9621" checksum="F6F232B94BC92F93" modified="2010-06-15" version="1">MRPTNTKLFSSPSFPASAKKKNSKMRAIRHTLRKLKVLLHLRHGKKTAIPRIVLTPPSESESDREEDDGEGSVGSRLDLRLGQGSGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEJ4</accession>
    <name>D5GEJ4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00006519001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430175">
        <property type="protein sequence ID" value="CAZ82937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838746.1">
        <property type="nucleotide sequence ID" value="XM_002838700.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEJ4"/>
    <dbReference type="GeneID" id="9181568"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006519001"/>
    <dbReference type="HOGENOM" id="CLU_3260853_0_0_1"/>
    <dbReference type="InParanoid" id="D5GEJ4"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="42" mass="4916" checksum="54586D6732511103" modified="2010-06-15" version="1">MDCSLIERRSECGGRERIGRGEREEEKPPGEKTRQLEQIAVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GER0</accession>
    <name>D5GER0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein-serine/threonine kinase</fullName>
            <ecNumber evidence="10">2.7.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00001337001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the PDK/BCKDK protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.-" evidence="10"/>
    <dbReference type="EMBL" id="FN430185">
        <property type="protein sequence ID" value="CAZ83003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838812.1">
        <property type="nucleotide sequence ID" value="XM_002838766.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GER0"/>
    <dbReference type="FunCoup" id="D5GER0">
        <property type="interactions" value="333"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GER0"/>
    <dbReference type="GeneID" id="9184915"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001337001"/>
    <dbReference type="eggNOG" id="KOG0787">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023861_4_1_1"/>
    <dbReference type="InParanoid" id="D5GER0"/>
    <dbReference type="OMA" id="WSYPPSA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004740">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010906">
        <property type="term" value="P:regulation of glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.20">
        <property type="entry name" value="Alpha-ketoacid/pyruvate dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036784">
        <property type="entry name" value="AK/P_DHK_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018955">
        <property type="entry name" value="BCDHK/PDK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039028">
        <property type="entry name" value="BCKD/PDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947:SF20">
        <property type="entry name" value="[3-METHYL-2-OXOBUTANOATE DEHYDROGENASE [LIPOAMIDE]] KINASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10436">
        <property type="entry name" value="BCDHK_Adom3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69012">
        <property type="entry name" value="alpha-ketoacid dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Histidine kinase/HSP90-like ATPase" evidence="11">
        <location>
            <begin position="230"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ83003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="392" mass="42304" checksum="5E8861D0FF1C1A95" modified="2010-06-15" version="1">MVGLGVPWRGILHPRPVELKGLDSLVSPSSFGRPPLTTQSLLTSARFTREYLPVRLSRRIRALRNLPYIIVSNPNISTIYNNYIDSLTAILAFPAGSLTNLTEESKFTELLTEIVKTHSNTIPILAKGFLECRGYVTPEEATKFLDHHLRARIGTRLMAEQHIGLHLASVPQPPTVSVPGKSYIGTVDTELRPAEIIESCASFVGDICELRYGTRPKLTIDGNPDITFPYIPVHLEYIITELLKNAFRATIEANSPVPVIATIAAAPGPPDAGGITIRIRDQGGGIAPDDLCHIWSYSFSTFNGSRSSIGSGNSGRLEDVDDGGADGGVDVMGRFTESAGALETSSIAGLGYGLPMSRAYAQYFGGSLKVQSAYEWGTDVYLRLKGVGLDGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GES7</accession>
    <name>D5GES7_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">carbonic anhydrase</fullName>
            <ecNumber evidence="2">4.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00001352001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 11">
            <text>hydrogencarbonate + H(+) = CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:10748"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="EC" id="4.2.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the alpha-carbonic anhydrase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.1" evidence="2"/>
    <dbReference type="EMBL" id="FN430187">
        <property type="protein sequence ID" value="CAZ83020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838829.1">
        <property type="nucleotide sequence ID" value="XM_002838783.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GES7"/>
    <dbReference type="STRING" id="656061.D5GES7"/>
    <dbReference type="GeneID" id="9184936"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001352001"/>
    <dbReference type="eggNOG" id="KOG0382">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039326_0_2_1"/>
    <dbReference type="InParanoid" id="D5GES7"/>
    <dbReference type="OMA" id="GHTIQAN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03124">
        <property type="entry name" value="alpha_CA_prokaryotic_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.200.10">
        <property type="entry name" value="Alpha carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041891">
        <property type="entry name" value="Alpha_CA_prokaryot-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001148">
        <property type="entry name" value="CA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036398">
        <property type="entry name" value="CA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023561">
        <property type="entry name" value="Carbonic_anhydrase_a-class"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952:SF265">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00194">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01057">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51069">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51144">
        <property type="entry name" value="ALPHA_CA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072742" description="carbonic anhydrase" evidence="7">
        <location>
            <begin position="23"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-carbonic anhydrase" evidence="8">
        <location>
            <begin position="33"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ83020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="282" mass="31994" checksum="756446C87CB05E86" modified="2010-06-15" version="1">MRSFVLSSLFLSVSLPSALVSACLYKRADPSLPSYTYIGETGPLLWHNLTVDGKNFPYRKCATGKQQSPIDITDSVPLAEDTRTTFPFSGEFTIEDNGHTIEVTPQKPDDYKSILGGQEYKLSQFHFHISSEHLLRHETLPLEVHFVHTKVDDDKKIAVIGIFFDLVDIGAGDMLLQSIFPKLDDLEKPKHRRRGEEKSEDYFKVQTHSINNIVDNSHVRSYPGSLTTPPCSEEVSWYVVEEPLSITVKQLSKMRKIMKFNSRVTQNDYANPPRENLLALGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>D5GET7</accession>
    <name>D5GET7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00001380001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430193">
        <property type="protein sequence ID" value="CAZ83030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838839.1">
        <property type="nucleotide sequence ID" value="XM_002838793.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GET7"/>
    <dbReference type="STRING" id="656061.D5GET7"/>
    <dbReference type="GeneID" id="9184944"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001380001"/>
    <dbReference type="eggNOG" id="ENOG502R71Z">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1526276_0_0_1"/>
    <dbReference type="InParanoid" id="D5GET7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ83030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="176" mass="19495" checksum="6DA573A9A0E74A18" modified="2010-06-15" version="1">MDLLEKTKGRVVVKPALHWLYATLYKGAAFGYPQAAAEETGYGSELEAAIKGLKGMHRRGITILPRGDCGCAWTTHGTYASDLAHFAKHLDFMPMEYILAATAEAGKLFIQAITQTAFSSMGIFWGISPFCRTTRGWTSLWLMEGCTRVASFRFIVIYRLLERVKGRLTLSPSSCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEX1</accession>
    <name>D5GEX1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00006628001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430197">
        <property type="protein sequence ID" value="CAZ83064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838873.1">
        <property type="nucleotide sequence ID" value="XM_002838827.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEX1"/>
    <dbReference type="STRING" id="656061.D5GEX1"/>
    <dbReference type="GeneID" id="9188602"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006628001"/>
    <dbReference type="eggNOG" id="ENOG502QUQ4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026011_0_0_1"/>
    <dbReference type="InParanoid" id="D5GEX1"/>
    <dbReference type="OMA" id="WHITSAF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006307">
        <property type="term" value="P:DNA alkylation repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.590">
        <property type="entry name" value="Alpha-ketoglutarate-dependent dioxygenase AlkB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027450">
        <property type="entry name" value="AlkB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037151">
        <property type="entry name" value="AlkB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032854">
        <property type="entry name" value="ALKBH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31212">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT DIOXYGENASE ALKB HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31212:SF4">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT DIOXYGENASE ALKB HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13532">
        <property type="entry name" value="2OG-FeII_Oxy_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="2">
        <location>
            <begin position="252"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="121"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ83064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="431" mass="46805" checksum="00097635CB771DCF" modified="2010-06-15" version="1">MPPSRKKLKKPLDPSFFEPKSQRDRRGSAENSATARAAAGGCSTAKYVSEEGDEQLDTDTKIAILASLHPSLSTAALIDILLSVNGSIEATSKLLAAGQTTPQARKPPSTTNQSSLETFISTQGTSTTTSTTTSTPKPPPKGQTLHLYTPASIALHTPCVLHTTFLPPPLATTLLQELLHESTTFPPPGKFNLFSRTVQSPHRSVMYTTTPATTPYYYQGLTLPPPRAFTPSMSTAAGLVEEHFRTLEPRGRITAALVNCYAGPQQGVGFHTDALTYIGPRAIICTLSLGVTREFRIQKQPPSSHSLGTYAIHLPHNSLLVMTAGMQENWKHSIPLVQRVDRHEISGDIRIAITFRWYRDDYGPEVTPACGCGGRMVLRPVWGGEKYFWSCDGEYRGVGQGCGYFKWAVFGEGGRPVFEDEKGKVVEGGDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>D5GF22</accession>
    <name>D5GF22_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00006708001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430197">
        <property type="protein sequence ID" value="CAZ83115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838924.1">
        <property type="nucleotide sequence ID" value="XM_002838878.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GF22"/>
    <dbReference type="STRING" id="656061.D5GF22"/>
    <dbReference type="GeneID" id="9187976"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006708001"/>
    <dbReference type="eggNOG" id="ENOG502S2UQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_448482_0_0_1"/>
    <dbReference type="InParanoid" id="D5GF22"/>
    <dbReference type="OMA" id="PFNDPVN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="367"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="50"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="252"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="423"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="518"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="74"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="260"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="310"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="353"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="444"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="468"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="524"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="542"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="561"/>
            <end position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ83115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="609" mass="63056" checksum="C5E6723EAB7A0850" modified="2010-06-15" version="1">MRRDAYARHRREGVEGIEVVTDVVFVTASVSGGGKVIGFTTLGEAVEKTLSPSPTTVAPPKVTSKAASSERPRATTSETLKATEAIRTQSAISSTSESTEVASPTSTLATSTSAIASNSESILALTSTSASASETSIAPIASITSSAAPSSEESASGGMSTGATVGLAVAGVGVFLVICFTLAFVYRKRSQKKTQEAYGKPEDEKTGGAAAVGATIYMNGNNNLSRADTVTSEKAPRVPSLRPVTQFLPNLTVTPPAADPVSQRQSQAQVQSRPQQRAPVIASSPVGLTRSESRKAPPPALVLVKSEGEVSSAPVSSATTTNSTAPLLAKTQTSPTASSSSSLVEYTDSGDSAAGTPVAGGPAGVANTSPVHRVQMDFKPSMEDELGLRVGQLVRLLYEYDDGWALCIRLDRSQQGVCPRTCLSQRPVKPRHPGSPRPGYPNGRAQSPIGSQGSPRPYSPSGVPRAQSPGSNRSQLSQSPVQMRPQSPAQAHPKSPAGYVKQVYRPATATQQAQLRIIPASPVQRPQSPITQSPVALRAGPSQITVTTTTPAVISEAAPSTPVSPAQKPQSLPGSPPRPAVAPVRPSPLSNEQTSGHEPEHAQPVFHAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFB9</accession>
    <name>D5GFB9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00006816001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430208">
        <property type="protein sequence ID" value="CAZ83212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839021.1">
        <property type="nucleotide sequence ID" value="XM_002838975.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFB9"/>
    <dbReference type="GeneID" id="9188068"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006816001"/>
    <dbReference type="HOGENOM" id="CLU_359496_0_0_1"/>
    <dbReference type="InParanoid" id="D5GFB9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ83212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="779" mass="88497" checksum="19FACED33EFE77DC" modified="2010-06-15" version="1">MNRPTRPKGSHYQPPRPDGAFFNSILEPFTAIFSPFSALPPEAPCAPFSTVQESFELLDARILAGKSTSTAIKEYKTLKTIEPGLRLTGPAVIVLLRNLLKLGVPTPILEVLEMENLMVDEGIRLLRVAMEGLSLEQRRRVAGRLLEAEGHLHRKRFRTILSEEIKSFETEEVIVRNGSEYVKDIKKAQSRNEMRKLLDEIVLTKVAITRRTSQLIASRLVDLNELGLGVSYALEPLINISQSNFIATICDALCDANQYGRGYELIMKALKTGKYGLLSDLDMKRGVRTIDTMSQPHLQLYNLWDAMKSSSSIYPRDLSYNTYAAFLGRATKLRFGLRGSLREGGGETPLLTMSIEIIVRFTDQEKKMARGLISSMLEAWIWSWGSTCSKKPDFQILEDSIPFMAPLFNILPPESRDPAILMAVERTTTNCIRSGREVWPRGIIWPFVSTLAQAKIWQEEESIGLWATERVLLRKRLAPTYLRYRTDAEIARFVARHYLPLRLGLTGPKRTTRQTGDAERLMVRMRSLERGAASFPSLSVFALAILAFKDTCISSTTLLIDMLYTLHRLHRYAEIPPLLASLKFYNISPPRSDIARLIRLLTRAHPTSALELLQIYPDTQYSVFAQFIVRTATLHPDLALRAYQLLTPPTLHYFSRFQPKIPGRRLPGRKLLVAMGWNFANSPVLSPRQCLRYAQRCLRAMLVLGFDPGPRMGWAIAVAGVERAIHAEIPVRREWERMKWVLETVRRNAGPKRAREVDLILSRWEKNVWNGSVSRRGGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFS8</accession>
    <name>D5GFS8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="11">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00007057001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430220">
        <property type="protein sequence ID" value="CAZ83371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839180.1">
        <property type="nucleotide sequence ID" value="XM_002839134.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFS8"/>
    <dbReference type="STRING" id="656061.D5GFS8"/>
    <dbReference type="GeneID" id="9187282"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007057001"/>
    <dbReference type="eggNOG" id="KOG1341">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_463212_0_0_1"/>
    <dbReference type="InParanoid" id="D5GFS8"/>
    <dbReference type="OMA" id="LFALMMY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140107">
        <property type="term" value="F:high-affinity potassium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003445">
        <property type="entry name" value="Cat_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004773">
        <property type="entry name" value="K/Na_transp_Trk1/HKT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051143">
        <property type="entry name" value="TrkH_K-transport"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00934">
        <property type="entry name" value="2a38euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31064:SF30">
        <property type="entry name" value="HIGH-AFFINITY POTASSIUM TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31064">
        <property type="entry name" value="POTASSIUM TRANSPORT PROTEIN DDB_G0292412-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02386">
        <property type="entry name" value="TrkH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0630">Potassium</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="206"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="249"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="278"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="341"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="469"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="51"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="127"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="570"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="51"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="93"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="573"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ83371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="589" mass="65989" checksum="C824DC284150C4E3" modified="2010-06-15" version="1">MSEVGVWRAESVCIVYPFYNIYLLPLSWKLTTVLSHLEKVVLRAADGSALPTQTTQTSSINDTGTTPHSPEAPTSGRIVWDVPASHSTQNAHQSEEEKAHYYDTSRSTAGAIRRSSVDTIHEITDTGDITRVDTGPASSSSRRVRALSRENSLSRNRPSFRKSRDDPPAPYLSYNPSVGRNSRFYDLTEEQRNELGGIEYRSLKTLAWILIAYYAFIHVFGVIMLVPYLLRSLKYGKILDDFGVNKSWWGAYTAMSCFANVGFLLTPDSMVSFQRASLVLIVSGFLIIAGNTGFPLLLRFTIWCLFKLCPDRYPDKRDELNFLLDHPRRCFTLLFPSGPTWWLFWILIILNIADTVLFIVLDLQTPVVESIPAGYRVLNALLQAVSTRTAGVSSVPIGSLHPGIQCSYLIMMYISVLPLAISIRRTNVYEEHSLGIYIPSAEAREAQEEKRDEGTYIKAHIRRQLEFDLWYVFLGLFLICVAEGRRIADASDYAFTAFSVFFEVVSAYGDVGLSLGYPGLNTSLSGTFTTFSKLVIVAMEIRGRHRGLPYDVDRAVLLPNVTTPDSMFAPDGARLEGVKSRNGEKDEEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>D5GG05</accession>
    <name>D5GG05_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007137001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430231">
        <property type="protein sequence ID" value="CAZ83448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839257.1">
        <property type="nucleotide sequence ID" value="XM_002839211.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GG05"/>
    <dbReference type="GeneID" id="9181793"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007137001"/>
    <dbReference type="HOGENOM" id="CLU_797379_0_0_1"/>
    <dbReference type="InParanoid" id="D5GG05"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="171"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="348" mass="37968" checksum="F29856A5A1EDC56C" modified="2010-06-15" version="1">MVYVPWRLSSLEACHVSEEEWAGLRSTVIEALNTSTIGLEGPSVHGTAWDDWFEELAERVAREYWPKTDRVAVRRIFRPQVIEMAGVERGKRRAQRAGGSEDTDGGAGSSRARPKREDEEDEEMGNAPNVSTSTATVPRAATLPSTPRESANVIPSPSSAPNPRTEEVEDTSSAATNTLTPLEAPRRGPGRPKKPPVNPPTEPSDTPTIVRKHPPQNPHEEVNPTPPKRPRTSRAHPPPPWIAPRPLEAPSAPSATLGLILETRIPPSEKMKIIYASDTPTLALFLEKVRKKFHLASEAQIHGVEVEIVGGKTYVVDLEDGRDWLAVQEIARTAGTSAVGVVVEVQSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5GG34</accession>
    <name>D5GG34_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]</fullName>
            <ecNumber evidence="10">1.3.1.88</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">GSTUM_00001974001</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs. Specifically modifies U47 in cytoplasmic tRNAs. Catalyzes the synthesis of dihydrouridine in some mRNAs, thereby affecting their translation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>5,6-dihydrouridine(16) in tRNA + NAD(+) = uridine(16) in tRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53380"/>
            <dbReference type="Rhea" id="RHEA-COMP:13543"/>
            <dbReference type="Rhea" id="RHEA-COMP:13544"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.88"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="15">
            <dbReference type="Rhea" id="RHEA:53382"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>5,6-dihydrouridine(16) in tRNA + NADP(+) = uridine(16) in tRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53376"/>
            <dbReference type="Rhea" id="RHEA-COMP:13543"/>
            <dbReference type="Rhea" id="RHEA-COMP:13544"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.88"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="13">
            <dbReference type="Rhea" id="RHEA:53378"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>5,6-dihydrouridine(17) in tRNA + NAD(+) = uridine(17) in tRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53372"/>
            <dbReference type="Rhea" id="RHEA-COMP:13541"/>
            <dbReference type="Rhea" id="RHEA-COMP:13542"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.88"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="12">
            <dbReference type="Rhea" id="RHEA:53374"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>5,6-dihydrouridine(17) in tRNA + NADP(+) = uridine(17) in tRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53368"/>
            <dbReference type="Rhea" id="RHEA-COMP:13541"/>
            <dbReference type="Rhea" id="RHEA-COMP:13542"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.88"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="17">
            <dbReference type="Rhea" id="RHEA:53370"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 5,6-dihydrouridine in mRNA + NAD(+) = a uridine in mRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:69851"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:17789"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="14">
            <dbReference type="Rhea" id="RHEA:69853"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a 5,6-dihydrouridine in mRNA + NADP(+) = a uridine in mRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:69855"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:17789"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="16">
            <dbReference type="Rhea" id="RHEA:69857"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the Dus family. Dus1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.88" evidence="10"/>
    <dbReference type="EMBL" id="FN430237">
        <property type="protein sequence ID" value="CAZ83477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839286.1">
        <property type="nucleotide sequence ID" value="XM_002839240.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GG34"/>
    <dbReference type="FunCoup" id="D5GG34">
        <property type="interactions" value="645"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GG34"/>
    <dbReference type="GeneID" id="9184543"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001974001"/>
    <dbReference type="eggNOG" id="KOG2335">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013299_5_1_1"/>
    <dbReference type="InParanoid" id="D5GG34"/>
    <dbReference type="OMA" id="RPWWICQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102262">
        <property type="term" value="F:tRNA-dihydrouridine16 synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102263">
        <property type="term" value="F:tRNA-dihydrouridine17 synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02801">
        <property type="entry name" value="DUS_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035587">
        <property type="entry name" value="DUS-like_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018517">
        <property type="entry name" value="tRNA_hU_synthase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11082">
        <property type="entry name" value="TRNA-DIHYDROURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11082:SF5">
        <property type="entry name" value="TRNA-DIHYDROURIDINE(16_17) SYNTHASE [NAD(P)(+)]-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01207">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01136">
        <property type="entry name" value="UPF0034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="DUS-like FMN-binding" evidence="19">
        <location>
            <begin position="29"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="461"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CAZ83477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="483" mass="54250" checksum="9F634D7D0E89FD08" modified="2010-06-15" version="1">MASEVPPKTKPKLTGRDFYNSLGSPKTAVAPMVEQSELAWRLLSRRHSPPNTLCYTPMFHSRLFATTPIYRSQSFYPPLDGSKPDRPLFVQFCSNTPEDLFSAAQHVAPHCDGVDLNLGCPQGIARKGKYGAFLQEDWGLIRSLVSKLHKDLEVPVTAKIRILETRERSLEYARMVVDAGAQVLTVHGRTRDQKGHNTGMADWGVIRYIRDNLPKEVVLFANGNILWHEDVQRCLDATGADGVMSAEGNLYNPAIFQSDEDWEKRFPRMDILGREYLDIVRKEVLPGLEERGRKLLLQDPSITAIKPHLFKLWHALLPKYTDVRALLAKSSARASEGGDVLEPYETCLMEVQKIIKAELNQNPELTDENGRWVGPDTPFEEGAKGVMIEVDGVKFNRLVPWYRCQPYYRPLPEEAIKKGALNVRKSAQCQGISEKKKQKLDTGTGAQLVTERLQQAIKDERDIAKGVEVPGTSTGMESDVRAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GG37</accession>
    <name>D5GG37_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00001977001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430237">
        <property type="protein sequence ID" value="CAZ83480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839289.1">
        <property type="nucleotide sequence ID" value="XM_002839243.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GG37"/>
    <dbReference type="GeneID" id="9184541"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001977001"/>
    <dbReference type="HOGENOM" id="CLU_1846566_0_0_1"/>
    <dbReference type="InParanoid" id="D5GG37"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ83480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="139" mass="14853" checksum="311767CBC8CBC798" modified="2010-06-15" version="1">MRSHQCPQVPASLPAFSGEFNDPWVVCSPDMPEPRRSSYPRMSSCSIGPSIHRGTREASICATTSVGKNTAPLSLPVPQARANSSSRPGITPAMCPKIRNSINCPISPMCGENTLWWGCSGYSANPSGLSSQRSFLGVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>D5GG57</accession>
    <name>D5GG57_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="15 21">Inosine-5'-monophosphate dehydrogenase</fullName>
            <shortName evidence="15">IMP dehydrogenase</shortName>
            <shortName evidence="15">IMPD</shortName>
            <shortName evidence="15">IMPDH</shortName>
            <ecNumber evidence="15 21">1.1.1.205</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">GSTUM_00007206001</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth. Part of the gene cluster that mediates the biosynthesis of mycophenolic acid (MPA), the first isolated antibiotic natural product in the world. Does not play a role in the biosynthesis of MPA, but is involved in self resistance to MPA, since MPA acts as an inhibitor of IMP dehydrogenases.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 15 21">
            <text>IMP + NAD(+) + H2O = XMP + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11708"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="EC" id="1.1.1.205"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 15">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="15">Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.</text>
    </comment>
    <comment type="pathway">
        <text evidence="15 21">Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homotetramer. Seems to be able to form heterotetramers composed from more than 1 of the 3 IMPDH gene products (IMD2-4).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 15">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15 20">Belongs to the IMPDH/GMPR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.205" evidence="15 21"/>
    <dbReference type="EMBL" id="FN430242">
        <property type="protein sequence ID" value="CAZ83500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839309.1">
        <property type="nucleotide sequence ID" value="XM_002839263.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GG57"/>
    <dbReference type="FunCoup" id="D5GG57">
        <property type="interactions" value="1057"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GG57"/>
    <dbReference type="GeneID" id="9184562"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007206001"/>
    <dbReference type="eggNOG" id="KOG2550">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022552_2_1_1"/>
    <dbReference type="InParanoid" id="D5GG57"/>
    <dbReference type="OMA" id="MGYCGAK"/>
    <dbReference type="UniPathway" id="UPA00601">
        <property type="reaction ID" value="UER00295"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003938">
        <property type="term" value="F:IMP dehydrogenase activity"/>
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        <property type="entry name" value="Aldolase class I"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 19" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 15 21" id="KW-0332">GMP biosynthesis</keyword>
    <keyword evidence="5 15 21" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 15 17" id="KW-0520">NAD</keyword>
    <keyword evidence="9 15 20" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8 15 18" id="KW-0630">Potassium</keyword>
    <keyword evidence="7 15 21" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="22">
        <location>
            <begin position="127"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="22">
        <location>
            <begin position="190"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="active site" description="Thioimidate intermediate" evidence="15 16">
        <location>
            <position position="341"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15 16">
        <location>
            <position position="444"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15 17">
        <location>
            <begin position="284"/>
            <end position="286"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15 17">
        <location>
            <begin position="334"/>
            <end position="336"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15 18">
        <location>
            <position position="336"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
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        <location>
            <position position="338"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
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        <location>
            <position position="339"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
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        <location>
            <position position="341"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
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    <feature type="binding site" evidence="15">
        <location>
            <begin position="374"/>
            <end position="376"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
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        <location>
            <begin position="397"/>
            <end position="398"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
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    </feature>
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        <location>
            <begin position="422"/>
            <end position="426"/>
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        <ligand>
            <name>IMP</name>
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        <location>
            <position position="456"/>
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            <name>IMP</name>
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        <location>
            <position position="511"/>
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        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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            <dbReference type="ARBA" id="ARBA00005502"/>
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            <dbReference type="ARBA" id="ARBA00022755"/>
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        <source>
            <dbReference type="RuleBase" id="RU003927"/>
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        <source>
            <dbReference type="RuleBase" id="RU003928"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51371"/>
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        <source>
            <dbReference type="EMBL" id="CAZ83500.1"/>
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    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
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    </evidence>
    <sequence length="530" mass="56784" checksum="A753BB224EC9043D" modified="2010-06-15" version="1">MSSTAANIGTLDFSTALSVLEKEYRRDGLDVHSLLDSSKRGGLTYNDFLILPGYIGFPASDVTLDSKITKRVSLKLPLVSSPMDTVTEEQMAIFMALLGGVGVIHHNCTAEDQAEMVRKVKKYENGFILDPVVMSPKNTVGDVRAVKEKQGFAGIPITEDGHLHSKLIGIVTMRDIQFHRDNSTALSEVMTTELLTAPEGINLSEANEILRSSKKGKLPIVDQAGNLTALLSRSDLMKNLHFPLASKRPYSKQLLCAAAIGTREDDKARLKKLVEAGLDIVILDSSQGNSMYQVSMIKWIKQEFPGIDVIAGNVVTREQAANLIAAGADGLRIGMGSGSACITQEVMAVGRPQAAAVHSICEFASRFGVPCIADGGIQNVGHIVKGLALGASTVMMGGLLAGTTESPGKYFLSAEGKLVKAYRGMGSIDAMESMKTGDNAATGRYFSEGDKIRVAQGVSGSVLDRGSVTKFVPYLQAGLQHSFQDIGVQSISELQQGVNYGIVRFEIRTVSAQAEGNVHGLHDFNKKLYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGL3</accession>
    <name>D5GGL3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00007546001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="CAZ83743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_002839506.1"/>
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    <dbReference type="AlphaFoldDB" id="D5GGL3"/>
    <dbReference type="STRING" id="656061.D5GGL3"/>
    <dbReference type="GeneID" id="9181713"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007546001"/>
    <dbReference type="eggNOG" id="KOG0395">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_9_2_1"/>
    <dbReference type="InParanoid" id="D5GGL3"/>
    <dbReference type="OMA" id="DMVNIEE"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:GTPase activity"/>
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        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Ras-related protein Rab-1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="entry name" value="Small_GTP-bd"/>
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        <property type="entry name" value="Small_GTPase"/>
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        <property type="entry name" value="Small_GTPase_Ras-type"/>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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    </dbReference>
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        <property type="entry name" value="RAB"/>
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        <property type="entry name" value="RAS"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="190"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="190"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ83743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="232" mass="25401" checksum="229F0C3F3932A1A3" modified="2010-06-15" version="1">METISITVCGDGGTGKSSISLRLVRSKWTHEYDPTVQDSYSVTRTVDGREYSLHLTDTAGQEEYRGLWASHNLAADAYLLVYDITNHDSLLALDYFHNLVEMEEEGRSVPVVKIVAGNKCDLAEGRQVSSQEGLAWARGHECGFMETSARNMVNIEETFALLVRRVVAAREAVANGTLVPASYRKFSSATAGPAASASGPVARDSEKQQEVPHLTYDNRPPPSKWGNCCTIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGN0</accession>
    <name>D5GGN0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007431001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430275">
        <property type="protein sequence ID" value="CAZ83652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839461.1">
        <property type="nucleotide sequence ID" value="XM_002839415.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGN0"/>
    <dbReference type="STRING" id="656061.D5GGN0"/>
    <dbReference type="GeneID" id="9185574"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007431001"/>
    <dbReference type="eggNOG" id="KOG1502">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_9_4_1"/>
    <dbReference type="InParanoid" id="D5GGN0"/>
    <dbReference type="OMA" id="FHFNIRM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051783">
        <property type="entry name" value="NAD(P)-dependent_oxidoreduct"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48079:SF6">
        <property type="entry name" value="NAD(P)-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48079">
        <property type="entry name" value="PROTEIN YEEZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="1">
        <location>
            <begin position="14"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="129" mass="14686" checksum="F00F488EFCC9819C" modified="2010-06-15" version="1">MAVENPTTTTKPKVLLTGANGYVAAHVLKQLVEANYHVTGTVRSEEKANLSRSFNPEYEGSVRFIVLPDFTKQESWDQIFKETEYDYVVHTAAPLMSEENTDFDKHYLKPNVQGYGSLCLFFTRRSVQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGU5</accession>
    <name>D5GGU5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00007517001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430275">
        <property type="protein sequence ID" value="CAZ83717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839526.1">
        <property type="nucleotide sequence ID" value="XM_002839480.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGU5"/>
    <dbReference type="GeneID" id="9181687"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007517001"/>
    <dbReference type="eggNOG" id="ENOG502SCPV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1012627_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGU5"/>
    <dbReference type="OMA" id="LCKTHGF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056119">
        <property type="entry name" value="DUF7702"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42109:SF2">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42109">
        <property type="entry name" value="UNPLACED GENOMIC SCAFFOLD UM_SCAF_CONTIG_1.265, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24800">
        <property type="entry name" value="DUF7702"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="150"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="185"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7702" evidence="2">
        <location>
            <begin position="3"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ83717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="275" mass="29758" checksum="27B95D1D912340C6" modified="2010-06-15" version="1">MTPRANLSLSEVILGALFLSLTAYNISRTPRETGWWYIAVFCLFRIVGSALLLTITLEQADTNKSLVIPATVFSGIAVSPLLAACLGFLNSCTQHLSTIYHPILRLLTLIHLLVLVALVLNAVGGVLVPLHSTPSPSPSAITSLNQAITYARASSLLFLTAWTFILLSLIILYRHWRAFAWTARVFYVAVLSGMPCLLVRILYAVLAAWDLGKGGVVTPGGGSRYGVLKGDVGVLVGMRVCMEFLVVAEWVAAGALEWWWGEVMREEESGGAKAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGW3</accession>
    <name>D5GGW3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00002067001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430284">
        <property type="protein sequence ID" value="CAZ83756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGW3"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002067001"/>
    <dbReference type="HOGENOM" id="CLU_2428668_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGW3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072778" evidence="1">
        <location>
            <begin position="20"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="91" mass="10625" checksum="263A9794195422E4" modified="2010-06-15" version="1">MTLLYELSLILHLLQVSQTKGFLIRRYLSECYNTNRSLYFKLLHLVYYVSANIRNCSSYACKSLVNSRGCLPDPIQKVRKFNADTIINFLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>D5GH34</accession>
    <name>D5GH34_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00007685001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430297">
        <property type="protein sequence ID" value="CAZ83827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839636.1">
        <property type="nucleotide sequence ID" value="XM_002839590.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GH34"/>
    <dbReference type="GeneID" id="9185081"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007685001"/>
    <dbReference type="eggNOG" id="ENOG502SEYQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_622856_0_0_1"/>
    <dbReference type="InParanoid" id="D5GH34"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072138" evidence="3">
        <location>
            <begin position="24"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="237"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="342"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="390"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="282"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="390"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ83827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="440" mass="47771" checksum="D0F53A178CF17285" modified="2010-06-15" version="1">MFISKKLRLAYIAEWLSIVSVSAVPVEHSPVVEEADGNMVVILGKREENSESLKRAIGEAVGLHHDIMARHDRNEDSDGDSKTEGGDLVHSADSSDKSLPGGRDNATLNSSQSSYTVAKKLVIAGGATAALILIVTIAFWLYKTKGRIFRRANSGHWKHYGAHRLRDTVYDEKSSVYPKSAIFGGGSSARPGMEREYSIPGMPFPPPAALAPPDTSVGRRQSRKHLLIRTSLLLPEGRPVCSPSPANEKGTFYDGASDVDSSSSLEQEELPIPPPVYTTDRLLLSPSQSSSISNVESRKPPTTPTNNRQPSYAQIYPPPPIPESNTTPRNAPIMVSRFSWTTTTTSEAPSGAKSVRSSLDSEPRFRGVNSWVSHQAGRLERGERVAQLQLEQQHGARNSSSPETPREFSHNPGAPVGFLAHRARVESAELDGRLSRGGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GH92</accession>
    <name>D5GH92_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00007801001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the oligoribonuclease family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430308">
        <property type="protein sequence ID" value="CAZ83917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839726.1">
        <property type="nucleotide sequence ID" value="XM_002839680.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GH92"/>
    <dbReference type="FunCoup" id="D5GH92">
        <property type="interactions" value="716"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GH92"/>
    <dbReference type="GeneID" id="9182622"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007801001"/>
    <dbReference type="eggNOG" id="KOG3242">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064761_3_1_1"/>
    <dbReference type="InParanoid" id="D5GH92"/>
    <dbReference type="OMA" id="AFFHYRN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06135">
        <property type="entry name" value="Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000003">
        <property type="entry name" value="Oligoribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022894">
        <property type="entry name" value="Oligoribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003765">
        <property type="entry name" value="PRK05359.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11046">
        <property type="entry name" value="OLIGORIBONUCLEASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11046:SF0">
        <property type="entry name" value="OLIGORIBONUCLEASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exonuclease" evidence="5">
        <location>
            <begin position="9"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ83917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="188" mass="21504" checksum="C98A977B2DEB5E78" modified="2010-06-15" version="1">MAPGASTAPLVWIDCEMTGLDPNEDVILQICCYITDHELNLLDEEGHETVIHYEKEVLVKMDDWCQKTHSSTGLISRVLESAVTVTQATEGLLAYIKKHVPEKGIAFLAGNSVHYDRVFLARYYPSVVEHLHHRIIDVSTVKEAVKMWCSEEVYGRVPEKKSLHEARMDILESIEELRYYKRVVFDGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHA6</accession>
    <name>D5GHA6_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 15">Histone-lysine N-methyltransferase, H3 lysine-79 specific</fullName>
            <ecNumber evidence="3 15">2.1.1.360</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13 15">Histone H3-K79 methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">GSTUM_00007723001</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 15">Histone methyltransferase that specifically trimethylates histone H3 to form H3K79me3. This methylation is required for telomere silencing and for the pachytene checkpoint during the meiotic cell cycle by allowing the recruitment of RAD9 to double strand breaks. Nucleosomes are preferred as substrate compared to free histone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 20">
            <text>L-lysyl(79)-[histone H3] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(79)-[histone H3] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60328"/>
            <dbReference type="Rhea" id="RHEA-COMP:15549"/>
            <dbReference type="Rhea" id="RHEA-COMP:15552"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="2.1.1.360"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.360" evidence="3 15"/>
    <dbReference type="EMBL" id="FN430308">
        <property type="protein sequence ID" value="CAZ83858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839667.1">
        <property type="nucleotide sequence ID" value="XM_002839621.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHA6"/>
    <dbReference type="FunCoup" id="D5GHA6">
        <property type="interactions" value="24"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GHA6"/>
    <dbReference type="GeneID" id="9182563"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007723001"/>
    <dbReference type="eggNOG" id="KOG3924">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027287_1_1_1"/>
    <dbReference type="InParanoid" id="D5GHA6"/>
    <dbReference type="OMA" id="IMEMTAL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140956">
        <property type="term" value="F:histone H3K79 trimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000033">
        <property type="entry name" value="Histone-lysine N-methyltransferase, H3 lysine-79 specific"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021162">
        <property type="entry name" value="Dot1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025789">
        <property type="entry name" value="DOT1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030445">
        <property type="entry name" value="H3-K79_meTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21451">
        <property type="entry name" value="HISTONE H3 METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21451:SF0">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE, H3 LYSINE-79 SPECIFIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08123">
        <property type="entry name" value="DOT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017570">
        <property type="entry name" value="Histone_H3-K79_MeTrfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51569">
        <property type="entry name" value="DOT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5 15" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 15 16" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="11 15" id="KW-0804">Transcription</keyword>
    <keyword evidence="10 15" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DOT1" evidence="17">
        <location>
            <begin position="48"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="177"/>
            <end position="180"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="200"/>
            <end position="209"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="262"/>
            <end position="263"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR017570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR017570-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAZ83858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="377" mass="42350" checksum="93AD00FDD4C668CE" modified="2010-06-15" version="1">MIANMGIREGGKGPSRIFRKTDQKLLSRGISTSDLDSINPRLTAFRYELVRLAEPDEFSPDLDILSTAEHVATYLLTPPESDKLLTGTLSGSSGGIVYNMRKAIRNEKAQDFLENLNKYNNLIAKYRRDGTIKANIARMKSLPFPLVEHILSQAYARTVAPEVESLKDYEAFTSNVYGELLPNFTTKLFRESGLGLGKVFVDLGSGTGNVVLHAALETGCEAYGCEIMKNATRLADRQKVEFAARCRMWGINPGKFRLEKGDFLESARIAEALKRADVVLVNNYVFDSELNQRLLNLFLDLKEGARILSLKPFVPINHVITTRNAENPVSRLRVEEKEYFSGSVSWTDTGGKYYVQTVDGSMLREFLASDRSDRRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHJ3</accession>
    <name>D5GHJ3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007944001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430319">
        <property type="protein sequence ID" value="CAZ83986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839795.1">
        <property type="nucleotide sequence ID" value="XM_002839749.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHJ3"/>
    <dbReference type="GeneID" id="9183640"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007944001"/>
    <dbReference type="HOGENOM" id="CLU_433603_0_0_1"/>
    <dbReference type="InParanoid" id="D5GHJ3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="247"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="461"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="530"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="129"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="182"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="220"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="268"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="472"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="631" mass="69461" checksum="3CCDCEBC962CDFEC" modified="2010-06-15" version="1">MPRATRNNSSGPLVSLPYGPRSKRKAPPVTEEERGLGEQEATTQGIGGFISPIVIDQDSESSPTLPSRPRKRVRATTLEVRGDDVVEAAEPAIEARLPLATRRPRRSPRKSLPWDIVAAGAQKAAGKPRSSAQNNRNRSSLATMEAREKGLAEETERRIEEGIPSEDEEGGGARLSASHDPLQLKEKQPADRHTTLLSPREEQAPSGSEDGHGDLLPADRSQESPEKVVEELMNNVVTSAIAQIDEIEEEEVEDLESESNDVEFNPRNYEDVDEAIRKQLSAEADGGENGEVEVGSEPVASGEDDPQCEMSLATALGAKLEKSREALQTFKEQLEATTGTANQLMELRDGIDSRSGYTRILREIIRKLAEEERPDNGALEALADFSLNISSMVSEIRDAHRDVEALLENLGAGIISGTRSAQMVETMLEQRGFEEEAPRDGINWKRINRNSVVRENEALASAKLNPVEPQTPMRSTRSNPSRYRTQGALPTPEARPPPSVPSSHDSLSDLEFLGSTAPVIGDDAPVEIVRIESFGSRPSNTPRRSKRRRESEDDDSPQVREDRAWSSPELKALFRRLAQVQKAEPGKVIVLDSRSGYTRTKEEILIKAKEIKDDMVRSGRAGTLKPIWDDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHR8</accession>
    <name>D5GHR8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">KRR1 small subunit processome component</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">KRR-R motif-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00008009001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Required for 40S ribosome biogenesis. Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly. Essential for vegetative growth.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3. Interacts with snoRNA U3. Interacts with MPP10, KRI1 and with ribosomal proteins RPS1A, RPS4A, RPS4B, RPS8A, RPS8B, RPS11A, RPS11B, RPS13, RPS24, RPS25, RPL4A, RPL7B, RPL8, RPL23, RPL25 and RPL28.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
            <location evidence="1 11">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the KRR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430320">
        <property type="protein sequence ID" value="CAZ84029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839838.1">
        <property type="nucleotide sequence ID" value="XM_002839792.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHR8"/>
    <dbReference type="FunCoup" id="D5GHR8">
        <property type="interactions" value="1043"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GHR8"/>
    <dbReference type="GeneID" id="9183683"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008009001"/>
    <dbReference type="eggNOG" id="KOG2874">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040185_0_2_1"/>
    <dbReference type="InParanoid" id="D5GHR8"/>
    <dbReference type="OMA" id="TPDIDKW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd22393">
        <property type="entry name" value="KH-I_KRR1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22394">
        <property type="entry name" value="KH-I_KRR1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000011">
        <property type="entry name" value="KRR1 small subunit processome component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000014">
        <property type="entry name" value="KRR1 small subunit processome component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041174">
        <property type="entry name" value="KRR1-like_KH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048550">
        <property type="entry name" value="KRR1-like_KH1_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048548">
        <property type="entry name" value="KRR1-like_KH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048549">
        <property type="entry name" value="KRR1-like_KH2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024166">
        <property type="entry name" value="rRNA_assembly_KRR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12581">
        <property type="entry name" value="HIV-1 REV BINDING PROTEIN 2, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12581:SF0">
        <property type="entry name" value="KRR1 SMALL SUBUNIT PROCESSOME COMPONENT HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17903">
        <property type="entry name" value="KH_KRR1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21800">
        <property type="entry name" value="KH_KRR1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006515">
        <property type="entry name" value="KRR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 11" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="6 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 11" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="K Homology" evidence="13">
        <location>
            <begin position="121"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="230"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="274"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="321"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="335"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR006515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ84029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="344" mass="40167" checksum="C83EBC25FDBE00A7" modified="2010-06-15" version="1">MPSTHDKEKPWDTDDIDKWKIEEFKPEDNKAGAFIEESSFATLFPKYRETYLREAWPLITRTLEKHHIACTLDLVEGSMTVKTTRKTYDPAAILNARDLIKLLARSVPVPQAIKILEDGVACDIIKIGNLVRNKERFVKRRQRILGPGGSTLKALELLTGCYILVQGSTVSAMGGYKGLKEVRRVVEGCMDNIHPIYHIKELMIKRELQKDPELVNENWDRFLPQFKKRTLSKRKKPRKITDKSKKTYTPFPPAPEKSKIDLQIESGEYFLSKQSKERAARDEREEKRKEKKEEKKKEREKDFVPPLEAGDRKNDKKKRKHEGETKEERKARKEERRRRKAAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHV9</accession>
    <name>D5GHV9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008139001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430321">
        <property type="protein sequence ID" value="CAZ84102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839911.1">
        <property type="nucleotide sequence ID" value="XM_002839865.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHV9"/>
    <dbReference type="STRING" id="656061.D5GHV9"/>
    <dbReference type="GeneID" id="9184195"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008139001"/>
    <dbReference type="eggNOG" id="KOG1208">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_44_4_1"/>
    <dbReference type="InParanoid" id="D5GHV9"/>
    <dbReference type="OMA" id="ETHGKWL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157:SF31">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="344" mass="37727" checksum="196C9F50A80A3BD3" modified="2010-06-15" version="1">MSTIFQKACAFFSSLPTPRTRIDGWRILITGANTGLGFEAAKHFARQGASVVLGCRSRDKGLNAKAQIDALLGLAPGIIQVSTVDMESFDSIRNFASSVGPVDGIVLSAGIASEIWNVTGDGWERTLQVNVLGTALLGRLLLPRALERRREVAGSGTIPTITIVSSHMYQFAEFKRYMKIEGNIIEALNDENRFGMGESDLMTPRRYSMTKLLDIYIARELAALVPEASDGGPMVIVNCVTPGFCHSDLMREYNLPLLGLWKALFARNTETGARTYFHSIVDAGPDSHGKYINNCKIESLPKKIFKTKEAKDLQRKVWDDIMEIVGDVGPEISQDNADPGSIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIA6</accession>
    <name>D5GIA6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00008370001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430324">
        <property type="protein sequence ID" value="CAZ84249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840058.1">
        <property type="nucleotide sequence ID" value="XM_002840012.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIA6"/>
    <dbReference type="GeneID" id="9181967"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008370001"/>
    <dbReference type="HOGENOM" id="CLU_2759648_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIA6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ84249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="70" mass="8154" checksum="636CDFB9AAD3CF4A" modified="2010-06-15" version="1">MYFPSPQAVCDRQLPISPVKLLISHFTLPFYNRILYAKIHPPLLAITNNNYLEFCYSSFVYPFFPLSASH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIG6</accession>
    <name>D5GIG6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00008491001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430326">
        <property type="protein sequence ID" value="CAZ84309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840118.1">
        <property type="nucleotide sequence ID" value="XM_002840072.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIG6"/>
    <dbReference type="GeneID" id="9184667"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008491001"/>
    <dbReference type="HOGENOM" id="CLU_2135347_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIG6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ84309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="113" mass="13057" checksum="41F28370E18B0EBF" modified="2010-06-15" version="1">MANRDPNFWQLFPSTPNVRTNGRKRNHQLQLASFESLASIYCNVYPLQYSAHHYQVASMDGGEPSTWRCFVVLHGVVPIMFWEIGQSMFCWQGRGVWFGGSITRKPKQLVFCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIS9</accession>
    <name>D5GIS9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00008659001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430328">
        <property type="protein sequence ID" value="CAZ84422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840231.1">
        <property type="nucleotide sequence ID" value="XM_002840185.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIS9"/>
    <dbReference type="GeneID" id="9185640"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008659001"/>
    <dbReference type="HOGENOM" id="CLU_3335910_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIS9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ84422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="38" mass="4339" checksum="79E96C0CF2A8A159" modified="2010-06-15" version="1">MVMVGDEAMCLQCAIENSREPFFFLELSLIKRGFSGRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIX2</accession>
    <name>D5GIX2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008724001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840274.1">
        <property type="nucleotide sequence ID" value="XM_002840228.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIX2"/>
    <dbReference type="STRING" id="656061.D5GIX2"/>
    <dbReference type="GeneID" id="9185683"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008724001"/>
    <dbReference type="HOGENOM" id="CLU_702468_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIX2"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="337"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="337"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="393" mass="43258" checksum="9ACCDDA34DBB6DDD" modified="2010-06-15" version="1">MENHLIYQGRTSLRNAPSNTSQPKLDAKEQGFSPSTPTPSLLGKPYTMGSFSWDRDCVQERGGARGATEHLGPGGDGGLLARDTRKRNYEKNPRRKMREAGRGIECDRKRKRGKDSGIMGGKHPKEARVQGHMLKRVLSPCNSRDRLTLPPTHKTGLFKKGRASRLVKSGLSDLVFSEMRFLATTGEDQVALDQGRTMSKGEERFCRPHALEISHSDRARKVSGAAHRDKLTQDRKEVQGKIKDRALLTKLSMLNAGHRTPSSPCVSSLGPKESTCNPGRGIRNCFDARKALLSPVNLARGRMMPGRGSEEKPKSTAMNASAVRTFQGLSGLIEEGQQNRVSGQPQAASVSPTLRTLERRGTPRKAGNGEFALQEYLGRRTGCSNDGEYKVWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJ69</accession>
    <name>D5GJ69_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00008865001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJ69"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008865001"/>
    <dbReference type="HOGENOM" id="CLU_2905805_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJ69"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ84562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="62" mass="7180" checksum="4A61DD76A2C3799D" modified="2010-06-15" version="1">MWEEIKTLSVSSQWRKNDVFLANRIFFQHSNVPDQRGQKTSHTQTVATPPGVATLMKTEIHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJV8</accession>
    <name>D5GJV8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00009228001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430334">
        <property type="protein sequence ID" value="CAZ84801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840610.1">
        <property type="nucleotide sequence ID" value="XM_002840564.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJV8"/>
    <dbReference type="GeneID" id="9186837"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009228001"/>
    <dbReference type="eggNOG" id="ENOG502STEZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1455409_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJV8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ84801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="186" mass="19412" checksum="DC2E64CE1C40D259" modified="2010-06-15" version="1">MPSSPKTTLTISSHQSTMKIQSIAIFVAAVFTGFAFASPVPGAEPAPAVDSVPCGCTNDCIIACAGRPNCNCPQFCDPKYACPTTTTAKPTTTTSPTPTFPPGTTCGCHNSCTLFGCGPDIPRDQCVCPQFCDPIYECPPTPTTTAPPRPTFTCGCTNDCKIACGGRPGCICPQFCDPKYECKTTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJY7</accession>
    <name>D5GJY7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00009274001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430335">
        <property type="protein sequence ID" value="CAZ84830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840639.1">
        <property type="nucleotide sequence ID" value="XM_002840593.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJY7"/>
    <dbReference type="STRING" id="656061.D5GJY7"/>
    <dbReference type="GeneID" id="9186866"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009274001"/>
    <dbReference type="eggNOG" id="ENOG502QRU3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2442479_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJY7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ84830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="90" mass="10288" checksum="D444064EC68C1176" modified="2010-06-15" version="1">MVEAKCAQVDAKQAAATCGDGQTQPRLNDEQWQALVALYRTLPHEHHDFFLASQHPSASPPLRRLADKYAMPARMWRHGICGFLKLLRYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GK38</accession>
    <name>D5GK38_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00009349001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the isochorismatase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430335">
        <property type="protein sequence ID" value="CAZ84881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840690.1">
        <property type="nucleotide sequence ID" value="XM_002840644.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GK38"/>
    <dbReference type="STRING" id="656061.D5GK38"/>
    <dbReference type="GeneID" id="9186918"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009349001"/>
    <dbReference type="eggNOG" id="KOG1211">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025343_0_0_1"/>
    <dbReference type="InParanoid" id="D5GK38"/>
    <dbReference type="OMA" id="VECCVTT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00431">
        <property type="entry name" value="cysteine_hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.850">
        <property type="entry name" value="Isochorismatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000120">
        <property type="entry name" value="Amidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000868">
        <property type="entry name" value="Isochorismatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036380">
        <property type="entry name" value="Isochorismatase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895:SF169">
        <property type="entry name" value="GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895">
        <property type="entry name" value="TRANSAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00857">
        <property type="entry name" value="Isochorismatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52499">
        <property type="entry name" value="Isochorismatase-like hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isochorismatase-like" evidence="3">
        <location>
            <begin position="24"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Amidase" evidence="4">
        <location>
            <begin position="280"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="86"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="88"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ84881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="707" mass="76006" checksum="1B30F0D0CEC54792" modified="2010-06-15" version="1">MEALPKNITIEAKPYPYTFPLSSTALLLIDLQRDFILPSGFGDIQSGMNLAAVTAVVPNCVRILQAFRELELPIFHTREGHLPDLSDCPSSKLNRQASAPGTNHSKVIGDPGKLGRLLVRGEHGHDIVDECQPKLGEVVVDKPGKGAFWNTNLSEELVGWGITHLIVGGVTTECCVTTTVREANDRGFECCIIEECAAGYNDSFQAPSLNMIHWSQGLFGFVSSLPNLLKELASVPSPGAPVPDLGLESPPSTPPIWDGRLTLAALRKSYRSGLPPVTVITGLYARIEDYSQTKSTFIHLVDRSALISYAESLQKRFPDLANLPPLYGVPFTLKDSINVAGIPTTLACPPLAHIPSRSSKVYSRLISLGAVYIGKTNLDQFATGLTGCRSPYGVPASVYNPDHVSGGSSSGSAVSVGAELSSFAVATDTAGSGRVPAGFNGVVGWKPTKGTVSFSGVMNACESLDCLSFMVTSAGGVKDVRKLWNLVRGYDPDDPYSKMPGSLPLPMVDALGGKRWKFAIPDRKAVAECSPEYRKLFYQAIGGLHEIGGEVKEGDWGLFEEAGRLLYDGALVNERLAALPDNKWVERERDELHPMTGLTRKVNATLFNPSHSSYIDVLIVPTAPFHPRISAVLKDPIAINSRLGTFTHFGNVLDLCAIAVPAGHYMEDEKKMPFSITFLGRGGFDARVLEIASLFEGLVGAGARDSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKD3</accession>
    <name>D5GKD3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00009487001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430337">
        <property type="protein sequence ID" value="CAZ84976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840785.1">
        <property type="nucleotide sequence ID" value="XM_002840739.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKD3"/>
    <dbReference type="STRING" id="656061.D5GKD3"/>
    <dbReference type="GeneID" id="9181996"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009487001"/>
    <dbReference type="eggNOG" id="ENOG502S522">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109915_1_0_1"/>
    <dbReference type="InParanoid" id="D5GKD3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37451">
        <property type="entry name" value="MARVEL DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37451:SF1">
        <property type="entry name" value="MARVEL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01284">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="67"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="6">
        <location>
            <begin position="3"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ84976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="117" mass="12880" checksum="D430F97438B7BA11" modified="2010-06-15" version="1">MVNPILLLRVTQGVLAFIVLGVAAYVVDGYDGAVDAANFLVFDSVWTFIALGYVVVTPMFFPNFHNRWAVLGVEAITMVFWFAGFVALAAGIDRLRCDRQGRRLHLGLLNGLLGQLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKS9</accession>
    <name>D5GKS9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">threonine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Threonyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">GSTUM_00009747001</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>tRNA(Thr) + L-threonine + ATP = L-threonyl-tRNA(Thr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24624"/>
            <dbReference type="Rhea" id="RHEA-COMP:9670"/>
            <dbReference type="Rhea" id="RHEA-COMP:9704"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78534"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.3" evidence="3"/>
    <dbReference type="EMBL" id="FN430341">
        <property type="protein sequence ID" value="CAZ85122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840931.1">
        <property type="nucleotide sequence ID" value="XM_002840885.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKS9"/>
    <dbReference type="STRING" id="656061.D5GKS9"/>
    <dbReference type="GeneID" id="9183358"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009747001"/>
    <dbReference type="eggNOG" id="KOG1637">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008554_2_0_1"/>
    <dbReference type="InParanoid" id="D5GKS9"/>
    <dbReference type="OMA" id="HRYEYSG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004829">
        <property type="term" value="F:threonine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070159">
        <property type="term" value="P:mitochondrial threonyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00771">
        <property type="entry name" value="ThrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000039">
        <property type="entry name" value="Threonyl-tRNA synthetase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002320">
        <property type="entry name" value="Thr-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033728">
        <property type="entry name" value="ThrRS_core"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451:SF50">
        <property type="entry name" value="THREONINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451">
        <property type="entry name" value="THREONINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01047">
        <property type="entry name" value="TRNASYNTHTHR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="14">
        <location>
            <begin position="81"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="30"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="33"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAZ85122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="459" mass="51737" checksum="34C9EDC49BCE89EB" modified="2010-06-15" version="1">MRRPALLIAQRLSIIQTCLHRIPSPRPIARASWSRSSHTLSPGSPKPGHTGGSPPDHRLLAAAQDLLITHPVSPGSPLFLPHGSRIFNKLISFIRSQYTLYGYEEVITPTIYKKSLWETSGHWENYNEHMFKVEAGEEFGLKPMNCPGHCLLFKHAAGKLSLRDLPVRYADFSPLHRNENSGSLSGLTRVRRFHQDDAHIFCRPSQISQEISSTLRFIQTVYKVFHLPPYKLRLSTRPKEGYMGSLEEWDRAEAALKASLDKSGQDWAINEGDGAFYGPKIDTILTDSDGKEHQTATIQLDFQLPRRLGLNYFAPAPAAEQKGILSTDPEQLKESGFVTPHYNGKWPFWLSPRQAIVIPVAMTDEIVAYARAMSRRTFNVDIDESGGLLSRKVRAARIKHYNYIIVVGEKNLQAKTVSLGSASPRHQEQGHEEGVEKGLKDMSVEEVYQKFVELENEYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKY2</accession>
    <name>D5GKY2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphodiesterase</fullName>
            <ecNumber evidence="3">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00009828001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="3">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cyclic nucleotide phosphodiesterase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="3"/>
    <dbReference type="EMBL" id="FN430342">
        <property type="protein sequence ID" value="CAZ85175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840984.1">
        <property type="nucleotide sequence ID" value="XM_002840938.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKY2"/>
    <dbReference type="STRING" id="656061.D5GKY2"/>
    <dbReference type="GeneID" id="9185795"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009828001"/>
    <dbReference type="eggNOG" id="KOG3689">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010668_4_0_1"/>
    <dbReference type="InParanoid" id="D5GKY2"/>
    <dbReference type="OMA" id="TETERCC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004114">
        <property type="term" value="F:3',5'-cyclic-nucleotide phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1300.10">
        <property type="entry name" value="3'5'-cyclic nucleotide phosphodiesterase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036971">
        <property type="entry name" value="PDEase_catalytic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDEase" evidence="5">
        <location>
            <begin position="224"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="19"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="609"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="716"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="611"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="734"/>
            <end position="754"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ85175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="794" mass="87524" checksum="2343335B7CFB6A14" modified="2010-06-15" version="1">MHQVDYNVVYVDRRARRDRVQHRDDLLPSPISSGPESSDGNDEAQNENEEVIHNLQCLLSAFNSVTICTTGTSCVSVLSDVDRNDPTPTLVLLDIPWQPQPEEDIVGHEGRHDSGIAADMLYGLPLLKFICNEVDALRMSSLVVPVTFLTNREPAAANGTLPNQICWSTTETERCCEERELKCIDNGAVDVLVSPIPRERAKVMYMHCYRARKNVQKSRRVSWVGIDEQKPVREASDYAYLREKMYAYFYRCGPHMRISSGNIEKIQLAIGSWNFSAHDFSDEELVHCAKLMLEHALEMPEVEAYRISSDRIVDFLTACRAAYNPKIPYHNFRHVVDVLQAVFYFLLQLRALPPYSPGEPVGLGPPPARSLSHLLTPLDALTLLVVAVGHDVGHPGVNNAFLITLKAPLAQVYNDRSVLESFHCAAFSQVLRRYWPKTVELRKSIIDMILATDMGLHFDYMGRLDEMKEKVLKDGGMDSLKDQAISDYRTLVCALLIKCADISNVARTHECSSQWAKILIEEFARQATMESDLGISSSPVAPPVTGSVLALAKSQVGFMTLFALPLFISLSEVLPEMQFSVDVLIANKAMWQTEIETITSASDGIVDKMFGNSNGPAPGSLSTSAGSKQGGEDLGDGRLGGSHGTHVLGPVVMESSTTFQSSGNLGGGGSEARLNVLALEPGVQASAEQPTVTVVVTQPKTLSPDPHALLDEKRPTHQYSSEHLGTQEPPEQDIPDRPRSSPPDLGDHSSEHSCSKACCGPTAVVSTSTIQRRPSSFFKRVKLWKSWRKETSEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5GL46</accession>
    <name>D5GL46_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00009988001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430346">
        <property type="protein sequence ID" value="CAZ85239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841048.1">
        <property type="nucleotide sequence ID" value="XM_002841002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GL46"/>
    <dbReference type="GeneID" id="9185833"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009988001"/>
    <dbReference type="eggNOG" id="ENOG502QR7D">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023537_1_1_1"/>
    <dbReference type="InParanoid" id="D5GL46"/>
    <dbReference type="OMA" id="WFAWCNS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008313">
        <property type="entry name" value="GH125"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31047">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 157 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31047:SF0">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 157 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06824">
        <property type="entry name" value="Glyco_hydro_125"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028846">
        <property type="entry name" value="UCP028846"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01149">
        <property type="entry name" value="DUF1237"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072415" evidence="1">
        <location>
            <begin position="21"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="514" mass="57673" checksum="2E5B6ADEF40D769D" modified="2010-06-15" version="1">MIGAATLLVSFLFLIGGSEANRQDALYRSQQQQFPSHHRACPDYRDYATRLHPPYSKGKYRLPFQRPVEECRLYNSPAVESLIAEMNARIKDKDLARLFENCFPNTLDTTVRWHLNSERVADKQSFIVTGDINAQWLRDSTNQLAQYQLLAKNDESLRNLILGAINTQADFVLQSPYCNAFQPPKASGLAPTSNGQDDNVHPAYDNTVVFECKYEIDSLASFLSLGNQYYNHTGDLSFVGEKWLSAVDNVMALIEEQQASSFSRTGLAQRQVYTFQRRTTLGTETLNLGGIGNPINHNTSLVRSAFRPSDDAAIMQFFIPGNAMLAVELKRTAEILRKVRQIELANFVQKKGEAIEAGVWKHGVITHPVFGKVFAYEVDGYGSHIIMDDANLPSLLALPVLGFVDVKNQTYQNTRKMLLSREGNPYFLTGTEFKGIGGPHIGITHAWPISLLIEILTTNDDDEILALLGMSVNVERVKDYTRSWFAWANSLFAQTILDLAHRKPQILFSVNGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLL5</accession>
    <name>D5GLL5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NCT transcriptional regulatory complex subunit A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Negative cofactor 2 AB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00010259001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4">Forms the NCT transcriptional regulatory complex with nctB and mot1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NC2 alpha/DRAP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430349">
        <property type="protein sequence ID" value="CAZ85408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841217.1">
        <property type="nucleotide sequence ID" value="XM_002841171.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLL5"/>
    <dbReference type="STRING" id="656061.D5GLL5"/>
    <dbReference type="GeneID" id="9182711"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010259001"/>
    <dbReference type="eggNOG" id="KOG1659">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_704361_0_0_1"/>
    <dbReference type="InParanoid" id="D5GLL5"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017054">
        <property type="term" value="C:negative cofactor 2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001046">
        <property type="term" value="F:core promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22906">
        <property type="entry name" value="HFD_DRAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000036">
        <property type="entry name" value="CBF/NF-Y family transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050568">
        <property type="entry name" value="Transcr_DNA_Rep_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252:SF5">
        <property type="entry name" value="DR1-ASSOCIATED COREPRESSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252">
        <property type="entry name" value="HISTONE-LIKE TRANSCRIPTION FACTOR CCAAT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="8">
        <location>
            <begin position="260"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="190"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="345"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="215"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="350"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="375"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00061393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00065307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00072430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00075891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ85408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="392" mass="42144" checksum="9DB5E88AE1CD62BB" modified="2010-06-15" version="1">MRLDGAQREYGRADGMGRLDRLGEAEEGWRKWEKRVRGGDLLSFRNFCYPEGGSFLSFNTPEKDPITLPYPLPPPQNSEISITRQSTFSIIPSYSSLHVDPPGQATHHPTRPLPATYPLTIENPTAHNPVHITGALHTHCRCRKTVAQPPMSHPTDEEMSGYYNNTHPITGVAGSLPPPFQPHQQLPSAVSIPLVPPTTPVTEKVGRGRGRRGRGAAAANNPNTPTTPFNTTFPANGESSSTGATGSAATGRTGVVIKTKFPVARIKRIMQADEDVGKVAQVTPVVVSKALELFMVSLCDKAALQARMRNSKRITAGHLKEAVLHEDQFDFLAEIIAKVPDIPLPAENQGGAGSGSGGDAEEGAGGGGDATPSVKKTRKPRQRKVKDEDAFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLM5</accession>
    <name>D5GLM5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">1-phosphatidylinositol 4-kinase</fullName>
            <ecNumber evidence="2">2.7.1.67</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00010272001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
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                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
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                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
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                <person name="Zampieri E."/>
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                <person name="Lebrun M.H."/>
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            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
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        <reaction evidence="1">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + ADP + H(+)</text>
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            <dbReference type="ChEBI" id="CHEBI:15378"/>
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            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58178"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
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        </reaction>
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            <begin position="245"/>
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</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLP5</accession>
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        <lineage>
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        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
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                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
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                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
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                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
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                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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            <end position="166"/>
        </location>
    </feature>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85438.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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    </evidence>
    <sequence length="196" mass="21463" checksum="4B0EA30C685BB924" modified="2010-06-15" version="1">MSVSDPIKPNPDNQPVPLISIPESSYIADLAEKAYEKGSTAFLRLKALSRVRDVVLRPEKQGKDIVWNRIANHEAILGHTRGKLQEKPCNTCVQKIGPFADCVAVTGEFLGSCTNCHYNSCGKRCSFRYPNQTPGSKRKRSLSDASFVESGGDCDPPAKTVKTRKGDRAQDFAVHWDGLSGIIRDLIVEKVFGTGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMD2</accession>
    <name>D5GMD2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00010657001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430354">
        <property type="protein sequence ID" value="CAZ85675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841484.1">
        <property type="nucleotide sequence ID" value="XM_002841438.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMD2"/>
    <dbReference type="STRING" id="656061.D5GMD2"/>
    <dbReference type="GeneID" id="9183245"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010657001"/>
    <dbReference type="HOGENOM" id="CLU_2943511_0_0_1"/>
    <dbReference type="InParanoid" id="D5GMD2"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ85675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="60" mass="6654" checksum="50009B34CC26EBBE" modified="2010-06-15" version="1">MRSIGASKLALSTISSEELWQSSGRLEKGLGELFRFEDRKDEITQATAIEALSNNSEVPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMD6</accession>
    <name>D5GMD6_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">PAN2-PAN3 deadenylation complex catalytic subunit PAN2</fullName>
            <ecNumber evidence="10">3.1.13.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">PAB1P-dependent poly(A)-specific ribonuclease</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Poly(A)-nuclease deadenylation complex subunit 2</fullName>
            <shortName evidence="10">PAN deadenylation complex subunit 2</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">PAN2</name>
        <name evidence="13" type="ORF">GSTUM_00010661001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalytic subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein PAB1. PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1. May also be involved in post-transcriptional maturation of mRNA poly(A) tails.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>Exonucleolytic cleavage of poly(A) to 5'-AMP.</text>
            <dbReference type="EC" id="3.1.13.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="10">Binds 2 metal cations per subunit in the catalytic exonuclease domain.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="10">Positively regulated by the regulatory subunit PAN3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Forms a heterotrimer with an asymmetric homodimer of the regulatory subunit PAN3 to form the poly(A)-nuclease (PAN) deadenylation complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">Contains a pseudo-UCH domain. This ubiquitin C-terminal hydrolase (UCH)-like or ubiquitin specific protease (USP)-like domain is predicted to be catalytically inactive because it lacks the active site catalytic triad characteristic of thiol proteases, with residues at the equivalent structural positions that are incompatible with catalysis, and it cannot bind ubiquitin. It functions as a structural scaffold for intra- and intermolecular interactions in the complex.</text>
    </comment>
    <comment type="domain">
        <text evidence="10">The linker, or PAN3 interaction domain (PID), between the WD40 repeats and the pseudo-UCH domain mediates interaction with PAN3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the peptidase C19 family. PAN2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.4" evidence="10"/>
    <dbReference type="EMBL" id="FN430354">
        <property type="protein sequence ID" value="CAZ85679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841488.1">
        <property type="nucleotide sequence ID" value="XM_002841442.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMD6"/>
    <dbReference type="FunCoup" id="D5GMD6">
        <property type="interactions" value="651"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GMD6"/>
    <dbReference type="GeneID" id="9183249"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010661001"/>
    <dbReference type="eggNOG" id="KOG1275">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002369_1_0_1"/>
    <dbReference type="InParanoid" id="D5GMD6"/>
    <dbReference type="OMA" id="GCISCEM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031251">
        <property type="term" value="C:PAN complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004535">
        <property type="term" value="F:poly(A)-specific ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06143">
        <property type="entry name" value="PAN2_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000028">
        <property type="entry name" value="PAN2-PAN3 deadenylation complex catalytic subunit PAN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03182">
        <property type="entry name" value="PAN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030843">
        <property type="entry name" value="PAN2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050785">
        <property type="entry name" value="PAN2-PAN3_catalytic_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048841">
        <property type="entry name" value="PAN2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028881">
        <property type="entry name" value="PAN2_UCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15728">
        <property type="entry name" value="DEADENYLATION COMPLEX CATALYTIC SUBUNIT PAN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15728:SF0">
        <property type="entry name" value="PAN2-PAN3 DEADENYLATION COMPLEX CATALYTIC SUBUNIT PAN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20770">
        <property type="entry name" value="PAN2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13423">
        <property type="entry name" value="UCH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 10" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="8 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 10" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 10" id="KW-0540">Nuclease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="USP" evidence="12">
        <location>
            <begin position="444"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="369"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1048"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="832"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="834"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="941"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="994"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ85679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1071" mass="120339" checksum="E9F663B376F3D9F9" modified="2010-06-15" version="1">MLLFRLHWHLTPTKNFYGLGASKVGLLLTLVPIFENIPVTVVNDRGVISLAARSLHFSIRRGLAQWNIKHAGFQNLSGFAYTNRGTSEILVGGSQSSMFTVNVDRGTVIGEYSTEDDIKVMKRNPRAIVYGTGKGDVKLLDMNTFKVVREFPQAHGSTISDLDTQGNTILTCGYSPRQGTYHLDTLVKVYDLRTNRALAPVGFPSGAAFVRMHPKLSAAAIVASQAGQIQVVDIENPAAIKLYHANVTSYITAIELAPSGDALALMDADGYLQLWGSPDKLRFTELMNPVEWPEPLVLPNVAIKDDSPLNSIGMPYYRDVLLSAWPPHEIFNVNKMPQKIDADILAASATKTGYAPYHKRTPRYCIESTKHTDPPGAVPKFLSQQAKDKGSRNSGDFDDGKLLYGDESRRQFEVPPVFRKVEIKYSKFGVDDFDFEFYNKTRFSGLETHISNSYMNPLLQMYKFTPLLRNLALHHTATACTKDDCMLCQMGFLFDMLDKANGQNCQATNFLKTFSSLPTADRHGVLEEDSSSGELLSSMIQSLNRFLLDQISMDQRTWQNDSRALEEAITIGATKSQRCNICGKESRPLGDTNVTDMIYPPKHSKPVQSFSALVKQSIQMDTVQKGWCDKCQRYQQQAMRKLVRRLPKIMVFNATTNEKSLTYVREHWSTPGWLPDEIGVLVTNGQLSVYQGEDLIIMRERCENLVTFDLVGFVAEITPEEKRNTHLISFVNVAFSSTDRTEESTWHIFNDFLVTPVRKEDALDFAPKWKMPSVICFQARDPSNAIDNSWRNHLDTSLLYIDYSFGYGYSERECKSLEQHEEPKPGTLVALDAEFVSMQNEETEVKADGSRSVVRPSRLGLARVSVLRGGGVDEGVAFLDDYIITREPVVDFLTEFSGIHVGDLDPSTSKHALVPLKVAYKRLWLLLNLGCVFVGHGLLKDFRIINIHVPKEQVVDTVDLFVLRSSQRKLSLRFLAWVVLREEIQLDTHDSIEDSYTALRLYKKYLEYSDAGVLEPMLNNIQIEGMKTGFKPPPRGFLAEHRTDTPSIVQEGRSEANTPQPRGGVPKWLGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GME5</accession>
    <name>D5GME5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00010672001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430354">
        <property type="protein sequence ID" value="CAZ85688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841497.1">
        <property type="nucleotide sequence ID" value="XM_002841451.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GME5"/>
    <dbReference type="FunCoup" id="D5GME5">
        <property type="interactions" value="99"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GME5"/>
    <dbReference type="GeneID" id="9183258"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010672001"/>
    <dbReference type="eggNOG" id="KOG3322">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007205_0_0_1"/>
    <dbReference type="InParanoid" id="D5GME5"/>
    <dbReference type="OMA" id="KALSPMC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005655">
        <property type="term" value="C:nucleolar ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039182">
        <property type="entry name" value="Pop1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055079">
        <property type="entry name" value="POP1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009723">
        <property type="entry name" value="Pop1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012590">
        <property type="entry name" value="POPLD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22731">
        <property type="entry name" value="RIBONUCLEASES P/MRP PROTEIN SUBUNIT POP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22731:SF3">
        <property type="entry name" value="RIBONUCLEASES P_MRP PROTEIN SUBUNIT POP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22770">
        <property type="entry name" value="POP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06978">
        <property type="entry name" value="POP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08170">
        <property type="entry name" value="POPLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Pop1 N-terminal" evidence="5">
        <location>
            <begin position="53"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="POPLD" evidence="6">
        <location>
            <begin position="508"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="domain" description="POP1 C-terminal" evidence="7">
        <location>
            <begin position="807"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="457"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF06978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF22770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ85688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="880" mass="98523" checksum="091FC08479F03A44" modified="2010-06-15" version="1">MPPSTPNPRKRGAAAMPISGPERKRAKLQDARTIAVQNTEQALKTGELDVPAFIKSREFEIEALQNAMKASKESSNKRAFQIVPRDMRRRTASHNVKRVPKRLRPRATREMQSDNTPTVSARRRRPSGWLRFRKETARKLQTMAKKKDITAHISAKISRSRRTENVLRQPPRAQTKFRKRQKHKTWLPTHVWHAKRAKMIVRWRFAVAETPTDKSYRVAHRASGMRGCIAWDESYFSTIMLRGKERDVKEVMKALCPRDGNLMSSKVIAGTRASDTFIYRAGRYPLDLIAPVKVIWCVPEDPEAPQEERIRKLLIRVHPSAFLELWEELLPTAKPLKVTVEDLRFEIGSIEITGPDATNSLLAVLNPFDATDGDSPSGIWKNLRGLTNPSFLPLGACLSFDVIDPRLRDPPRLPEDKRKFEEVQETIFRVTSTWSIDRTQPPSSLFSREARAVAVKSQSSQKKINKRKGEAVPGEYPSPLPTDPRIPIVLLATRRSSAEKGVSGAIGSWTILLPWKWVQPVWYGIVHSGSSVKFGGLDESRQINYENSNRHFPDDFPGTKAGIVEELRKGVERKEWWDKRPKGKKVVWGSVNIGNKKGEVGDGFVCDWAYLLKGKKIDTTQGNDSIELSTDTIESTEDIAPTGTTAFMSSTESTGATISMPAVESNVSIEKSEHPQKMMSSMDIDKPPPNLPVVSLSTPTLFTGTTTTTAISPPKPTPQQTHPWTIPSSMIRYILAVPNSPLPKPLATLHPKILSAGVFSIKLVLPQRSTPMPRTRIYSLPTNNPTLKAKWKAVMSRKSQGKRPGKATELPDVPDEEDLIGFVTTGDFNLKEGRGTGVGALSWQKVFGRREKLGEVVEKACIVRDVGSGIGRLAYWEVID</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMS2</accession>
    <name>D5GMS2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">glucan 1,3-beta-glucosidase</fullName>
            <ecNumber evidence="10">3.2.1.58</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Exo-1,3-beta-glucanase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00010908001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Successive hydrolysis of beta-D-glucose units from the non-reducing ends of (1-&gt;3)-beta-D-glucans, releasing alpha-glucose.</text>
            <dbReference type="EC" id="3.2.1.58"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.58" evidence="10"/>
    <dbReference type="EMBL" id="FN430359">
        <property type="protein sequence ID" value="CAZ85815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841624.1">
        <property type="nucleotide sequence ID" value="XM_002841578.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMS2"/>
    <dbReference type="FunCoup" id="D5GMS2">
        <property type="interactions" value="365"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GMS2"/>
    <dbReference type="GeneID" id="9185151"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010908001"/>
    <dbReference type="eggNOG" id="ENOG502QQE6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028820_2_1_1"/>
    <dbReference type="InParanoid" id="D5GMS2"/>
    <dbReference type="OMA" id="WVWITET"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004338">
        <property type="term" value="F:glucan exo-1,3-beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050732">
        <property type="entry name" value="Beta-glucan_modifiers"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000490">
        <property type="entry name" value="Glyco_hydro_17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16631">
        <property type="entry name" value="GLUCAN 1,3-BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16631:SF26">
        <property type="entry name" value="GLUCAN 1,3-BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00332">
        <property type="entry name" value="Glyco_hydro_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0134">Cell wall</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072882" description="glucan 1,3-beta-glucosidase" evidence="13">
        <location>
            <begin position="22"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00036824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ85815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="306" mass="33075" checksum="BF4F7D82F3D603E2" modified="2010-06-15" version="1">MKISVASAFMAAMMATEAAIAAPLEARAPKVSYFFGFNIGANKPNGDCKSTQDWTDEFNAIKSWNPEFTAIKLFSTTDCSTLANAVPAAKATGLKIWAGVWAVDKEKFGREKAGLEQAIRDHGTEWLVGVNVGSESLYRKEIHPGVLAEQIWDVKGMVQTALGASHIPVGSADTWTSWVDGANVDVIKACDVIVMNAFPYWQGAQVDGSQDVFQKAVSNTKAAIGEDKQLIIGETGWPTGGPDFGAAHPNVENLQKFYKSAGCSLRSSKTPHFWFSAFDEPNRGSDIEKNFGIATSDRNLKINVTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMU9</accession>
    <name>D5GMU9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chitin synthase export chaperone</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00010945001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CHS7 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430360">
        <property type="protein sequence ID" value="CAZ85842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841651.1">
        <property type="nucleotide sequence ID" value="XM_002841605.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMU9"/>
    <dbReference type="FunCoup" id="D5GMU9">
        <property type="interactions" value="40"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GMU9"/>
    <dbReference type="GeneID" id="9183441"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010945001"/>
    <dbReference type="eggNOG" id="ENOG502QRVH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050424_1_1_1"/>
    <dbReference type="InParanoid" id="D5GMU9"/>
    <dbReference type="OMA" id="TVWEVKD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022057">
        <property type="entry name" value="Chs7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35329">
        <property type="entry name" value="CHITIN SYNTHASE EXPORT CHAPERONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35329:SF2">
        <property type="entry name" value="CHITIN SYNTHASE EXPORT CHAPERONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12271">
        <property type="entry name" value="Chs7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="49"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="85"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="114"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="185"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="223"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="252"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ85842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="340" mass="37585" checksum="3B9CC819D3EF771F" modified="2010-06-15" version="1">MSFGSFQSICETASIPLCSLVGPYGSSRVMRPGIEALCYSRSIETANTIIFQLANDFVHILALIMCSIMVLHVRSKFTAVGRKEITSFFYLFMLLTVISLCLDSGVIPIGSAPYAYFTAVQAGLTSALCTCLLINGFIGFQVYEDGTALSVWLLRLSTVAMFIVTGGVAICTFQSWIGLSPTKTMALFVVLYVVNALFLFVYVACQVILVMATLEDRWPLGDIVFGVFFFVVGQVLLYAFSDKLCDKVSHYLDGLFFSSICNLLGVMMVYKYWDSITKEDLEFSVGTKTNVWEVKELIPEEDRRTTIFEHAGPTSDCATSTYNSSGNRVSQGYGGFSDRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMZ1</accession>
    <name>D5GMZ1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">MutS protein homolog 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00011039001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA mismatch repair MutS family. MSH3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430361">
        <property type="protein sequence ID" value="CAZ85904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841713.1">
        <property type="nucleotide sequence ID" value="XM_002841667.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMZ1"/>
    <dbReference type="FunCoup" id="D5GMZ1">
        <property type="interactions" value="739"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GMZ1"/>
    <dbReference type="GeneID" id="9183472"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011039001"/>
    <dbReference type="eggNOG" id="KOG0218">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002472_0_0_1"/>
    <dbReference type="InParanoid" id="D5GMZ1"/>
    <dbReference type="OMA" id="INMHAAR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006312">
        <property type="term" value="P:mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.110:FF:000008">
        <property type="entry name" value="DNA mismatch repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1170.10:FF:000006">
        <property type="entry name" value="DNA mismatch repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1420.10:FF:000004">
        <property type="entry name" value="DNA mismatch repair protein Msh3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1420.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.10">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.110">
        <property type="entry name" value="MutS, connector domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007695">
        <property type="entry name" value="DNA_mismatch_repair_MutS-lik_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016151">
        <property type="entry name" value="DNA_mismatch_repair_MutS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036678">
        <property type="entry name" value="MutS_con_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003810">
        <property type="entry name" value="PRK05399.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF122">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MSH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01624">
        <property type="entry name" value="MutS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05192">
        <property type="entry name" value="MutS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55271">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="DNA repair protein MutS, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="11">
        <location>
            <begin position="1007"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="191"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="32"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="50"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="86"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="126"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="210"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="235"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS00486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ85904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1161" mass="128460" checksum="884CB3A88F526D6A" modified="2010-06-15" version="1">MGKSKTPSNAPGKAPQGKQRMISSFFAPKQSPGKTSTDNSSATPTIKKRKPDEKENKTADQEENEREPDSPPRTRRRVTQKRVLEDAEDVEQATDDDDYYSKPGTTSIAKKKLKRSPEPMANLESGGEKNEETRKKEANFLAPAAASRTKKAADSGRTLKYLYNPFADAMDIDGEPVDSATKKRREKLHEKFVQKLGKPGPVPEAHGVEVEEEAAEQDPEEPEDDEPGPIAKRFGGSAKGKAPPKGGKKLTPLERQVVEIKRKNPDTLLVIEVGYKFRFFGEDARTASQVLSIMCIPGKMRFDEHSSESHLDKFASASIPTHRLHVHVKRLVTAGHKVGVVRQVETAALKAAGDNRNAPFERKLTNLYTKGTYIDDVDGLDGDLAAGAGSGGAAGTGFMLCIAEKPGGGTGMDEKAHVGIVAVQPATGDVIYDEFDDGFMRSEIETRLLHIAPCEFLIVGELTKATEKLVSHLAGSTTTVLGDQIRIERVEGKKDAKILAPSHVSKFYADKLKSADSPEAAESNRLLEIVMNLPDLVTICLSALITHLSSYGLEHIFDLTKYFKSFSARSHMLLNGNTLSSLEIYRNQTDFSTKGSLFWTLDRTKTKFGKRQLRKWVGRPLLDKERLEERIEAVEEIKGGKSPKLERLRELLSKISYDLEKGLIRIYYGKCSRPELLSILQTLNRIANTFPPVDKPEDVGFNSKILNEALANLPRIKDDVEAYLDVFNHSQAAKDDKYDFFKDGDNYEAINEHKMGIAGVEGDLNEHLNEIAAVLKRKVVYVSVSGVEYLVEVSNEKNILKSVPATWVKMNGTRKVSRFHTPEVIKLLRERDQHKESLAAECNRAFAKFLAEISTKYQEFRDCVQSLATLDCLISLAAVANQPGYVKPEFTEEPCIEVREGRHPMVEQLLLDAYVPNDIHLFSDKQRAMIVTGPNMGGKSSFVRQVALIAIMAQVGSYVPASAARVGMLDAVFTRMGAFDNMMAGESTFMVELNETSDILKQATNRSLVILDELGRGTSTHDGVAIANAVLDYVITELKSMCLFNAMNAHMKFEEANDGTENITFLYQIGEGVAHRSYGLNVARLAGLPQRCIEGNPRTTPPFIPLTSETNTHPVAGVKSSQLEKELKERQTTKWAKTILSATEKEADFDPLQLLASIENL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GN62</accession>
    <name>D5GN62_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00011104001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430362">
        <property type="protein sequence ID" value="CAZ85955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841764.1">
        <property type="nucleotide sequence ID" value="XM_002841718.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GN62"/>
    <dbReference type="GeneID" id="9185881"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011104001"/>
    <dbReference type="HOGENOM" id="CLU_1866597_0_0_1"/>
    <dbReference type="InParanoid" id="D5GN62"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ85955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="137" mass="14726" checksum="F4363F63AB93C1FC" modified="2010-06-15" version="1">MSCQFWNPRQPFLPSHPTQRRPKSALLSTKNPVLRLNVMTYTGLTLYSSAKTARELLLLYISGIASCLSSSHGFVLGIEGSGDHYAKYGLDRHDGYGDHIFGVADLGVVNGVLGPSVSGILMGWTAVPEGCPGWASM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>D5GN67</accession>
    <name>D5GN67_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00011109001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CTAG/PCC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430362">
        <property type="protein sequence ID" value="CAZ85960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841769.1">
        <property type="nucleotide sequence ID" value="XM_002841723.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GN67"/>
    <dbReference type="GeneID" id="9185886"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011109001"/>
    <dbReference type="eggNOG" id="ENOG502S9SP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113770_3_1_1"/>
    <dbReference type="InParanoid" id="D5GN67"/>
    <dbReference type="OMA" id="VQYRATT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000408">
        <property type="term" value="C:EKC/KEOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070525">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.50">
        <property type="entry name" value="Alpha-D-phosphohexomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015419">
        <property type="entry name" value="CTAG/Pcc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31283">
        <property type="entry name" value="EKC/KEOPS COMPLEX SUBUNIT PCC1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31283:SF5">
        <property type="entry name" value="EKC_KEOPS COMPLEX SUBUNIT LAGE3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09341">
        <property type="entry name" value="Pcc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="106" mass="11705" checksum="C81D421E7A5A1FEE" modified="2010-06-15" version="1">MATATITAPPPVDPEYPHSLTISLPLPSHHLADTIQRALSVDREISSFVQRTYTSTPDSPILQIHYKATTARMLRVATNGMFESLGTVLRVCEELDVDTLEKELGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNI6</accession>
    <name>D5GNI6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00011305001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430365">
        <property type="protein sequence ID" value="CAZ86079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841888.1">
        <property type="nucleotide sequence ID" value="XM_002841842.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNI6"/>
    <dbReference type="STRING" id="656061.D5GNI6"/>
    <dbReference type="GeneID" id="9186155"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011305001"/>
    <dbReference type="eggNOG" id="ENOG502SP9C">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_987621_0_0_1"/>
    <dbReference type="InParanoid" id="D5GNI6"/>
    <dbReference type="OMA" id="NGIANDH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072171" evidence="3">
        <location>
            <begin position="23"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="54"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="83"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="154"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="184"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="192"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="233"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ86079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="282" mass="30952" checksum="DFF5AAF1D292AE48" modified="2010-06-15" version="1">MTAARAIKFVTLIFRLLQLLLAAYIVGVAGYHTWEFATNRLSLTDASSGQPPKALYTIFVISCVTVLYTSLSLFTTCFGHKKFFLATLIMDFLLLGGSIAISILMRGSPNMSCDPLEYSDSITSSSNDFPSNIYRSSGGSGNLAGTLCQLDKSVFAFAVALSVLFLITLLLSYLALRNHRKNRAFGPGPENGYSTRNNQAPRSWKRNPFNRTSEETAHTHNTNMSGVAAGMASHDDHGYEHRPSELGRGYRNSRQTPTLNPPEEPGPNVSPDYGRVAPHQMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNT6</accession>
    <name>D5GNT6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00011474001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430368">
        <property type="protein sequence ID" value="CAZ86179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841988.1">
        <property type="nucleotide sequence ID" value="XM_002841942.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNT6"/>
    <dbReference type="STRING" id="656061.D5GNT6"/>
    <dbReference type="GeneID" id="9182814"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011474001"/>
    <dbReference type="eggNOG" id="KOG1039">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_729959_0_0_1"/>
    <dbReference type="InParanoid" id="D5GNT6"/>
    <dbReference type="OMA" id="CHYKHET"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16521">
        <property type="entry name" value="RING-HC_MKRN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045072">
        <property type="entry name" value="MKRN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11224:SF10">
        <property type="entry name" value="IP09428P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11224">
        <property type="entry name" value="MAKORIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="8">
        <location>
            <begin position="52"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="104"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="8">
        <location>
            <begin position="200"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="52"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="200"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="308"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="309"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ86179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="379" mass="41915" checksum="C34F11143743DB6F" modified="2010-06-15" version="1">MEKPTSKKTPSPTAQPFVPGHQVTRASSFASTPIVASPNVDAAASKPRVNLPASLVPCRYFAAGYCQRGPACFYKHDLSSVTLPGLESADKGKKTHEPENTDTCAICYEIPVTYGLLTSCDHVFCLSCIRAWRSTAAPDAVPDSEGGALSKTSKTCPLCRVRSKFIIPSSVFPRPAGDAPDEGEKINPAKDAIIAAYLAKLKTIPCRHFTNSLLPSAPPRPRRNLPDCPFGNDCHYKHTFRGSPYVFSDSELSAQRRRRAHSPRRLGWMERLLFGAGYDSGSDPLPSPPWPVSEAEARTLMEIILEGGDPPEWDEFEEEEEDDEDDEDYFYEDVDDPFDPNWEQSILDTMEFSAPPRFNSRSRWAQGTGAGDQDDVFLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5GP54</accession>
    <name>D5GP54_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="13">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00011708001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the OXA1/ALB3/YidC family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430373">
        <property type="protein sequence ID" value="CAZ86319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842128.1">
        <property type="nucleotide sequence ID" value="XM_002842082.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GP54"/>
    <dbReference type="FunCoup" id="D5GP54">
        <property type="interactions" value="559"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GP54"/>
    <dbReference type="GeneID" id="9186559"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011708001"/>
    <dbReference type="eggNOG" id="KOG1239">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029282_5_0_1"/>
    <dbReference type="InParanoid" id="D5GP54"/>
    <dbReference type="OMA" id="HYTIHAT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032977">
        <property type="term" value="F:membrane insertase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032979">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane from matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20069">
        <property type="entry name" value="5TM_Oxa1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001708">
        <property type="entry name" value="YidC/ALB3/OXA1/COX18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028055">
        <property type="entry name" value="YidC/Oxa/ALB_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428:SF66">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEIN OXA1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428">
        <property type="entry name" value="OXA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02096">
        <property type="entry name" value="60KD_IMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="316"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="Membrane insertase YidC/Oxa/ALB C-terminal" evidence="12">
        <location>
            <begin position="162"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="406"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="441"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ86319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="479" mass="53268" checksum="5C8674FFA94F4CAB" modified="2010-06-15" version="1">MGPAYLPLNTNHRGELYHYTIHATIYAKRETMLASKLILRSSSSAASISLRRISQPASSRKISSFVFPRASRLPQPLLSKTFPKLAPLTALRFSSTSGTTPPLSEPTFLPDYTPTTQSVSAPIPTSEHIGFLKEFGLDYGYGFTTLVQSLIEHVHVYAGTPWFATIILSVGVLRLLQFPFYLKMSDTAAKMKELNPFAVPLTKKMRDAQIAGDTPGMVAARTELGQLYSRSGVKRRWLFFPISQVPVFYGFYKNLRGMSDIPIPELTEGGLSWFTDLSVADPFYLLPMATSASLFAQLSLGGEAGTNLQTEQMKKVLLIFLPLVTFVFTSQWPAVLTFYFLCNSLFSLVQTIALKNAWVRAKFGLYPMPNKAVPNPFEALPFKTMPAPTSTLPEVRQIGGKGSWMDRITGGSGSSNTDVTKSDKTEDGGFLEGMFNGGGEKAAHKAYEAKRQERIAKERDAKWELRLRKKRMQHEQNQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5GP69</accession>
    <name>D5GP69_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00011732001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the adrenodoxin/putidaredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430373">
        <property type="protein sequence ID" value="CAZ86334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842143.1">
        <property type="nucleotide sequence ID" value="XM_002842097.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GP69"/>
    <dbReference type="FunCoup" id="D5GP69">
        <property type="interactions" value="417"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GP69"/>
    <dbReference type="GeneID" id="9186574"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011732001"/>
    <dbReference type="eggNOG" id="KOG3309">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082632_0_1_1"/>
    <dbReference type="InParanoid" id="D5GP69"/>
    <dbReference type="OMA" id="TLGWGWR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016653">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, heme protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006784">
        <property type="term" value="P:heme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140647">
        <property type="term" value="P:P450-containing electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001055">
        <property type="entry name" value="Adrenodoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018298">
        <property type="entry name" value="Adrenodoxin_Fe-S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23426:SF65">
        <property type="entry name" value="FERREDOXIN-2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23426">
        <property type="entry name" value="FERREDOXIN/ADRENODOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00355">
        <property type="entry name" value="ADRENODOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00814">
        <property type="entry name" value="ADX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="7">
        <location>
            <begin position="82"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ86334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="197" mass="21646" checksum="AFF7159D27AC6C71" modified="2010-06-15" version="1">MPHLCIHSTNCTTIPPSPQIMFPRAIITPTARLLLRQPHLHLPLRTSPPPLPILPSILARSFSTTLLRKHGHTTPPKPGEELYVTFIDKSSNPHKFAVSAGENLLDIAQEHDLDMEGACGGSAACSTCHVIVADDAMYDRIPEPEDDENDMLDLAFGLTETSRLGCQVKMSKEIDGLVVKLPSMTRNLQSSDFSKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPH6</accession>
    <name>D5GPH6_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">chitin synthase</fullName>
            <ecNumber evidence="2">2.4.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00011848001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.4.1.16" evidence="2"/>
    <dbReference type="EMBL" id="FN430375">
        <property type="protein sequence ID" value="CAZ86419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842228.1">
        <property type="nucleotide sequence ID" value="XM_002842182.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPH6"/>
    <dbReference type="STRING" id="656061.D5GPH6"/>
    <dbReference type="GeneID" id="9186659"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011848001"/>
    <dbReference type="eggNOG" id="KOG2571">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1020112_0_0_1"/>
    <dbReference type="InParanoid" id="D5GPH6"/>
    <dbReference type="OMA" id="HRQSINY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004100">
        <property type="term" value="F:chitin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013616">
        <property type="entry name" value="Chitin_synth_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08407">
        <property type="entry name" value="Chitin_synth_1N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Chitin synthase N-terminal" evidence="7">
        <location>
            <begin position="216"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="161"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="164"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ86419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="273" mass="30479" checksum="3943A7138CCE18B7" modified="2010-06-15" version="1">MAGRDNNDAYSRLDFDGPIHHHDSDDDETGPLAQQQHRQSHIPVAATSPVMDPRYRTPSPAHALAGYQLEEAPYRPQRQNTGGYPAGSAQMPGDTLAYQPSYSVTNLEHSYNTAPPGPPSRTGHRQSINYSQGLYPEGLRDNYSVDPYEPHPPDHTYPLTQYTTHHDDYHDDDDRRPILEPDSPHRTPGDASYFPRNIPSPVSSAPTPGPGLRRWKTVKKVELFRGNLVLDCPVPNKLLGVLPSKAEREFTHMRYSAATCDPSENLVIRSSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPQ0</accession>
    <name>D5GPQ0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Small acidic protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00011969001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SMAP family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430377">
        <property type="protein sequence ID" value="CAZ86493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842302.1">
        <property type="nucleotide sequence ID" value="XM_002842256.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPQ0"/>
    <dbReference type="GeneID" id="9186725"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011969001"/>
    <dbReference type="HOGENOM" id="CLU_736072_0_0_1"/>
    <dbReference type="InParanoid" id="D5GPQ0"/>
    <dbReference type="OMA" id="VENCANS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026714">
        <property type="entry name" value="SMAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028124">
        <property type="entry name" value="SMAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22175:SF0">
        <property type="entry name" value="SMALL ACIDIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22175">
        <property type="entry name" value="SMALL ACIDIC PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15477">
        <property type="entry name" value="SMAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Small acidic protein-like" evidence="4">
        <location>
            <begin position="305"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="331"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="119"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="152"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="222"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="258"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="350"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="366"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF15477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ86493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="376" mass="40261" checksum="7B44ADB046DFE0DE" modified="2010-06-15" version="1">MSPEGNFAGINPERLKMLQARENPAPRDFSGRGRDRGWGQGGRGAGSGSGRGGGRGGYRATGRRFVFNEGAGSPAVANGGTKESTPAEEQLSGAEMRLAFKRNQERKSGAVVENCANSVKEEERKRKRDDEGAVAAESSPPKTSKRHKDSKTNGVTAATNGAPTLDEKRKHKKKKEKRDDRAKRAKRHAIEVGEVVVPSPSGARSSKQLNDDPDLSKEAKKAAKKERKEKREKRRAAIIEEPVNGDVGPSEHTSGSKTKTKKDKKPKKGKSKEPSSSSTDPTPAKRSKKSKTEILAGEAEYKWTAVAADLPGDDQRKSKFLRLLGAGAGALAATVPTANGSASLSSSSAKTREKDLERQYDAGLKVKGKKRRGLGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPX8</accession>
    <name>D5GPX8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00012084001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430380">
        <property type="protein sequence ID" value="CAZ86571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842380.1">
        <property type="nucleotide sequence ID" value="XM_002842334.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPX8"/>
    <dbReference type="GeneID" id="9185186"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012084001"/>
    <dbReference type="HOGENOM" id="CLU_2177200_0_0_1"/>
    <dbReference type="InParanoid" id="D5GPX8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="18"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ86571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="110" mass="12403" checksum="74AB755DF003FE71" modified="2010-06-15" version="1" fragment="single">FITGDSRELLHSIVRPRALSLAVIAGEVVSFFLLFYRLSPDIFFINEEITADRQNGTELISFSLVSTLFFLRFLLSFIFTCLLGVIWEWVVFTFCSYSPVAGEDGYDAGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GQ38</accession>
    <name>D5GQ38_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">tRNA (uracil-O(2)-)-methyltransferase</fullName>
            <ecNumber evidence="3 11">2.1.1.211</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00012186001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Adenosyl-L-methionine (AdoMet)-dependent tRNA (uracil-O(2)-)-methyltransferase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>uridine(44) in tRNA(Ser) + S-adenosyl-L-methionine = 2'-O-methyluridine(44) in tRNA(Ser) + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43100"/>
            <dbReference type="Rhea" id="RHEA-COMP:10339"/>
            <dbReference type="Rhea" id="RHEA-COMP:10340"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74478"/>
            <dbReference type="EC" id="2.1.1.211"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the TRM44 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.211" evidence="3 11"/>
    <dbReference type="EMBL" id="FN430382">
        <property type="protein sequence ID" value="CAZ86631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842440.1">
        <property type="nucleotide sequence ID" value="XM_002842394.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GQ38"/>
    <dbReference type="FunCoup" id="D5GQ38">
        <property type="interactions" value="82"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GQ38"/>
    <dbReference type="GeneID" id="9185211"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012186001"/>
    <dbReference type="eggNOG" id="KOG3790">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018580_2_0_1"/>
    <dbReference type="InParanoid" id="D5GQ38"/>
    <dbReference type="OMA" id="IREPNIN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141101">
        <property type="term" value="F:tRNA(Ser) (uridine(44)-2'-O-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002128">
        <property type="term" value="P:tRNA nucleoside ribose methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011671">
        <property type="entry name" value="tRNA_uracil_MeTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21210">
        <property type="entry name" value="TRNA (URACIL-O(2)-)-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21210:SF0">
        <property type="entry name" value="TRNA (URACIL-O(2)-)-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07757">
        <property type="entry name" value="AdoMet_MTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 11" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="7 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 11" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ86631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="456" mass="51634" checksum="4F5FBF210980FC01" modified="2010-06-15" version="1">METTIQPTPLYYQTTSDTLEQWTPILSKPCTFTQEIFTSVMHEMILNPNYNSSWILRADILSDSPTPPLQLDMSIPRPMLIPNFTPNRTVLRRFVPRNPQRDAAAEQTCTFYEGSQDRQALVVYQSHSDTPEALPYYLPTVRALAFWYSQDGEDGKSVSIYYRLFPDVPLNNRLERTALHLLTRIHKLGNGFTAGYTKRVYHDQIIPREHFQTTYLSLRERHAKRLIDRWVENTDPGKHVFEDILIAAFLIELWKDMDRGLEAGFVDIGCGNGVLVDILIREGYTGWGFDARRRKTWGVLGSSTEQNLHELVLVPRLLGGNVDDGIHDGVFPAGTFIISNHADELTPWTPLLAMDSECPFLMIPCCSHDLSGRRHRQPGKGSQYACLVEWVARLAEDVGWQIEREVLRIPSTRNVGIIGRKIGEGMTVESVLEREGGGSGWSERALALKAGGNKGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5G452</accession>
    <name>D5G452_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00003953001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TCP11 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835174.1">
        <property type="nucleotide sequence ID" value="XM_002835128.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G452"/>
    <dbReference type="GeneID" id="9182422"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003953001"/>
    <dbReference type="eggNOG" id="KOG1981">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016970_2_1_1"/>
    <dbReference type="InParanoid" id="D5G452"/>
    <dbReference type="OMA" id="FINAVRM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010737">
        <property type="term" value="P:protein kinase A signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008862">
        <property type="entry name" value="Tcp11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12832:SF11">
        <property type="entry name" value="LD23868P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12832">
        <property type="entry name" value="TESTIS-SPECIFIC PROTEIN PBS13 T-COMPLEX 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05794">
        <property type="entry name" value="Tcp11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="27"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="43"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="94"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ79295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="601" mass="68022" checksum="2489149F1BB61E15" modified="2010-06-15" version="1">MDSSPGPHRSHTRGQPFHLQPSEGEYDTSSVNTTSTQSIDIPESSSHNVSSEVSTPGYEYQSSPNAPAKRLNESAPPPETMYPLKRLKVEESDSSSSNCSLSECSMPAEAPAPDSESERSSRVKVAGNKQFRSRSLPPNLRRRRRTQQESIQQHIPPVTLNTLRELDLHEIYRNPKLRHDVVFDSQLHFRPNLDGTRGRRKKEMADMYWQIVLRECEVVLGSSKWRNAEMFSTTPRKLPILFETMRDILLTLVPKTDRSDVESILDPPLLMQQLKHNVLDFKGLSLWLATVLKAHCAPMRDQWVEQMVVKVAHGVDNRKVSALVEGIQMVFGILEAMKLDVANHQIRTLRPHLISTAISFEQGYFQDRIDQGRLDMKEPRSWFSNAVAARNTNEALDHMVIFTGAVVDMLASSQYAKYPATFVFDYERLEGLRDDIREATCLKMAILFFRQLTLSSKRDVDAATIDKLRTSIIAILGDEDGVHRWLRHSNAVALHVAQTAYEFNGSTGIADASTVKMAENWFAKHLQINSPIYVHVEKEISKELTGMVLRIIKGWSSLSTFSIMQSADVTGSSMELASIAQRASHIVFMHWRIFGKMYLAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4C9</accession>
    <name>D5G4C9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00004053001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835251.1">
        <property type="nucleotide sequence ID" value="XM_002835205.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4C9"/>
    <dbReference type="STRING" id="656061.D5G4C9"/>
    <dbReference type="GeneID" id="9184997"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004053001"/>
    <dbReference type="eggNOG" id="ENOG502SANM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_704358_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4C9"/>
    <dbReference type="OMA" id="DRSMHRD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058348">
        <property type="entry name" value="DUF8035"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26118">
        <property type="entry name" value="DUF8035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF8035" evidence="1">
        <location>
            <begin position="290"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF26118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="392" mass="45209" checksum="B850260C8531B94B" modified="2010-06-15" version="1">MGSESEDGYINAMPQNSSSQENIASCTVLYACCNTFSLTRSSHSSLGHLTEKAKEKHTAKHILQVCSVTFLGPPHNFVVHSTPLFSRGGFLKPPHPLPKSLRILYSIIFGTPKKRSLDMASISTITPYTSYGNPHTSTGALVRGGVIDEPVVEHEHHHVHHHIDHGNPVAGALVRPRVAREYSYDDLHDRERRYGSGMSLGSFGHHHGHHYGHRHRGLLGGRFARSDIDLNLDASYRHRRRSIYDDRDSELAIIDVPAGTRRVTVDMGREVDINWRRDNGVRRSRGLGSELWTEITKDLVTREAIEECGYPYEETEFFYYIFDYLHREQINELVDLTHEIRRHRIRDLEYESIGVGHPRFDRRLDYDREETRTEIVIEGGHRGHRRRNRYYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4F2</accession>
    <name>D5G4F2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00004082001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4F2"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004082001"/>
    <dbReference type="HOGENOM" id="CLU_2185858_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4F2"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ79395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="109" mass="12129" checksum="86DA4A2F640A6F5B" modified="2010-06-15" version="1">MRGEIVLSRVRIKPKPGVGESSTVTFKYARSNWCLNSPAQRGHWGSFGIPHKSSLPHLGLWASPALPGARKWAKGVSKPRPPQIGGEWRYCSNCEGRIVLRAQISERPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4H9</accession>
    <name>D5G4H9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00004113001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN429987">
        <property type="protein sequence ID" value="CAZ79422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842502.1">
        <property type="nucleotide sequence ID" value="XM_002842456.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4H9"/>
    <dbReference type="FunCoup" id="D5G4H9">
        <property type="interactions" value="140"/>
    </dbReference>
    <dbReference type="GeneID" id="9184051"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004113001"/>
    <dbReference type="eggNOG" id="KOG2533">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_0_1_1"/>
    <dbReference type="InParanoid" id="D5G4H9"/>
    <dbReference type="OMA" id="LWGICMT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000034">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000068">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF19">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G01812)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="173"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="206"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="281"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="314"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="341"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="365"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="401"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="433"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="50"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ79422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="493" mass="55390" checksum="2E0B7D802051CD24" modified="2010-06-15" version="1">MAEYSAKTASLEAKDRNGDYFEEADPDSGLSDAERKAIDKKLVRKLDMRLIPWLCLLYLASFLDRTNIGNAKIEGLVDDLGMTSGQYNACLTIFFVSYALFEPISNILLKRMRPRIYIPLIMVLWGICMTFMGFCRNFSEMMAARWFIPRYENSGVNYYLSCWYKRREFGIRAAIFFSAAAVSGSFGGLLAAAISKMDGVGGKRGWAWIFILEGLATALIGIISFWVVVDFPDDAKFLSPEDRARVLRRLRDDQQSSAEHEDFKTLYLWQSLKDWKTYTGMLIYMGVDGALYAFSLFLPSIIKEMGYSATRAQLLSVPPYAAACILTILVGWRADRNNRRGVFNIATSLLGVAGFSILLGSNSAALSYVAVFLGALGIYPCIPNTITWVSNNVEGVYKRGVTLGFVIGWGNLNGVMSSNVYRQVDAPRYRLGHGIVLGYLAIGLLGGSVLNWVLLRRENERRRNGERDAWVQGLNRKEVEYLGDQRPDFLYTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4Y1</accession>
    <name>D5G4Y1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00000151001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429993">
        <property type="protein sequence ID" value="CAZ79574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835417.1">
        <property type="nucleotide sequence ID" value="XM_002835371.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4Y1"/>
    <dbReference type="GeneID" id="9183056"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000151001"/>
    <dbReference type="HOGENOM" id="CLU_590777_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4Y1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ79574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="463" mass="53132" checksum="C44E4D6BD38673CF" modified="2010-06-15" version="1">MRTIYFDCVNKKFQWSKDDRFFFNVFDRERPERSKIGACHAGLIKMHEKTKIEKLNARLKTLRLFRRTITRGPEIRKMVVCWELGALFPMPMPGWRGRAALSKRGLKYRYPLADTLEKIGKLVEETIPYLTSLRWIDFHLDPEVVGASAEALSYIVKKSITRMTWKPRLEGCKCNHVRIKLPIGRTGYNDIPTQSYGLSEKSPSLEIYSDFDLPFYSPAIFKALATETCEVEISRPTRPSLRRDSPTVDAIDFPMTTAMRFIGAGAPTISQIIRREARRAATGTGMLTAITVGDRDIRFVDLRNLLAHSKNLKYLRLENVDVSAPTRGDIETSPRATVLKSATLKSVFWDIILRGEEDTRTETVTYYLVKSTSEACFPVLESLSGPAKKPYTEWWLSELRRVHPQKDIEMRSSENEVEMVLRPSTKAADEVGEQTRRRRFPARVLTFILARRTHQAHLVGPNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>D5G503</accession>
    <name>D5G503_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00000220001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429994">
        <property type="protein sequence ID" value="CAZ79596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835439.1">
        <property type="nucleotide sequence ID" value="XM_002835393.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G503"/>
    <dbReference type="STRING" id="656061.D5G503"/>
    <dbReference type="GeneID" id="9186293"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000220001"/>
    <dbReference type="HOGENOM" id="CLU_363361_0_0_1"/>
    <dbReference type="InParanoid" id="D5G503"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="244"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="330"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="504"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="615"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="506"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="622"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="687"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="728"/>
            <end position="738"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="769" mass="86692" checksum="7D044BE50527DD45" modified="2010-06-15" version="1">MLSRTVRVAHPRLARSIRVWGKRWETEIRDGKRMERRSEYVPGRGWVSERAEEEEHDDGGEIDLITLKRVPKAQNSWDPDADGKNCDHGGKTGFLDGFLQEPSSSASPPQGAKSVIPPQKSSVLPSGVAAATPTYPDPEQAPPPLPPRPTQPPSPPPRDKPEPSSPESDSGSESDSDMDILLPSDVRSSAGRPVKSKYEIEIERERRVRRDAMVQEWMQLREEQESAMHNIRAYRIMREQKEAQRKEAERKEIERREVERREAEEKEIQRREVERRVAEEKEMQRREEERREKLRLENQRTIELEIERGRILQEIKKRVRTESTAPLREDKEELDWSGTDTPAAEDTYKTPQYPPAQKYFVLTRNPSTNDFNFAPLPLALSPISPEQTTPIKVFENMQSPEKFIGYWDVVQRLGFEIVAGERECVIVRAPIEDADGKMQSMLQEIEGMRKLQTKPALKDNTPPKGLDAIAADIAGKIGLGGKSSPVVEEPPKVFEEFSSLVGETSKPVEKELAPTEQETAKPIEEAAKLQEVSSEAREPKAPEEDFKLNPSDIKRPDQIAEDAKYPEVVEENIESGQVHDGATATPPVKMVAEEPTEIETNNAWQDKYRFSENTEKTIQQPAEEKPFTEAPEVVKETLKKEEATKRWEATAAAPDPLASTRERTLTTQPLPLAETTPQPSPSPASEVARENWEKRRGSPLPPTPAEALLAKPAVEKPTLTTPAPAEAVNSQRQQGKSTQGKKPKRKIFWTSVWMAAATGGTSFLLENYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>D5G562</accession>
    <name>D5G562_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">Histone H2B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">GSTUM_00000270001</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the histone H2B family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429996">
        <property type="protein sequence ID" value="CAZ79647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835490.1">
        <property type="nucleotide sequence ID" value="XM_002835444.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G562"/>
    <dbReference type="FunCoup" id="D5G562">
        <property type="interactions" value="923"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G562"/>
    <dbReference type="GeneID" id="9186345"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000270001"/>
    <dbReference type="eggNOG" id="KOG1744">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075666_1_3_1"/>
    <dbReference type="InParanoid" id="D5G562"/>
    <dbReference type="OMA" id="FCPFAIR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22910">
        <property type="entry name" value="HFD_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000014">
        <property type="entry name" value="Histone H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000558">
        <property type="entry name" value="Histone_H2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055333">
        <property type="entry name" value="HISTONE_H2B_site"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23428">
        <property type="entry name" value="HISTONE H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00621">
        <property type="entry name" value="HISTONEH2B"/>
    </dbReference>
    <dbReference type="SMART" id="SM00427">
        <property type="entry name" value="H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00357">
        <property type="entry name" value="HISTONE_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6 13" id="KW-0158">Chromosome</keyword>
    <keyword evidence="10 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="12 13" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="11 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="15">
        <location>
            <begin position="30"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAZ79647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="138" mass="14914" checksum="10ABC92140CE8B53" modified="2010-06-15" version="1">MPPKAAEKKPTTAGKAPAGKAPEKKEAGKKTAAASGEKKKRSKTRKETYSSYIYKVLKQVHPDTGISNRAMSILNSFVNDIFERVATEASKLAAYNKKSTISSREIQTSVRLILPGELAKHAVSEGTKAVTKYSSSTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5C8</accession>
    <name>D5G5C8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00004272001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FRG1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835564.1">
        <property type="nucleotide sequence ID" value="XM_002835518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5C8"/>
    <dbReference type="STRING" id="656061.D5G5C8"/>
    <dbReference type="GeneID" id="9188140"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004272001"/>
    <dbReference type="eggNOG" id="KOG3962">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062276_2_0_1"/>
    <dbReference type="InParanoid" id="D5G5C8"/>
    <dbReference type="OMA" id="ACDVNGK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23339">
        <property type="entry name" value="beta-trefoil_FSCN_fungal_FRG1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008999">
        <property type="entry name" value="Actin-crosslinking"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010414">
        <property type="entry name" value="FRG1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12928">
        <property type="entry name" value="FRG1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12928:SF0">
        <property type="entry name" value="FSHD REGION GENE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06229">
        <property type="entry name" value="FRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50405">
        <property type="entry name" value="Actin-crosslinking proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="22"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="40"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ79721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="267" mass="29688" checksum="3CEA346C4D4DEA24" modified="2010-06-15" version="1">MVKALRFKGDKKVKKRKRTEVDNGNTEESSSSKVAKQQESEEPEGWVDSESLEDINGPIILTFSAARPICIACDAVGKVFASPIDSTEGEDLSTAEPTDVKQVWVATKIHGTEKYSFKSHHGRYLSCDKFGGLSATREAISPEEEFLPVLNELGSGRWALQTVRDKFLSVDEVGAGGATEIRGDAEAVGFKETFVVRLQARNKKRKKSGTEGKGKDKITRKELEEQAGVKLDDEQVKALKRARREGRFHEALLDIRVKFGKHDKFAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5H8</accession>
    <name>D5G5H8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00004342001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835614.1">
        <property type="nucleotide sequence ID" value="XM_002835568.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5H8"/>
    <dbReference type="FunCoup" id="D5G5H8">
        <property type="interactions" value="18"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G5H8"/>
    <dbReference type="GeneID" id="9183757"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004342001"/>
    <dbReference type="eggNOG" id="KOG0207">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001771_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5H8"/>
    <dbReference type="OMA" id="PMVVVIC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46594">
        <property type="entry name" value="P-TYPE CATION-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46594:SF4">
        <property type="entry name" value="P-TYPE CATION-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-1278">Translocase</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="161"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="189"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="230"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="266"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="423"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="457"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="771"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="803"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="8">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ79771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="827" mass="88744" checksum="9E1EFF245CCF292B" modified="2010-06-15" version="1">MTCTGCADKVVHALKSTSGVQKESVNVNFLMGRADLNFLPDFAHENEIMEQLKRSTGFRVVILDSGDMQDAGLAKAWISINSSSGGYRAIEGIDGMAKAKLLETAKGRHLFEAAYDPTVIGIRDLLAAINDRDHGTEAHLVPIPSEEMLTADLEKSHMKILGWKTVIAALLTIPVLIFAWAPLNIPRPSVYITQVFLATAVMGTAYKIYQSAWDTIVRGGAGGKRIDMDVLITVATGAAWAASVAILIISGRNGSLTPESQPVEPFFETCCLLVTLILTGRWITAKVRMWALKRVVDVGEEAPKDKNTTKAMLSVEGATPETISPDLLQYGDVLVAEKGDVIATDGVVVDGKAEVDESLLTGESKPAVMKAGSLVMAGSTVVTGSIRFRLTRLPHENTISVIRRMVRSAGGEKPKIQAIADKVALYLTPLILGVATTVFLIWILVHGLTRQETWGKSAVIAITYAVATLAVSCPCAIGLVVPMVIVFTSRVGIRKCGLLLVNPKALERGWNAKKVVFDKTGTLTTGKLNVVTAKCFLNGEWGGFDADAEQIVRTLCKNEKHPVARALAAKVGSGEEISGLEVETIIGKGLQAISERRTIRGGQPSWTATGGESHPLVLEIVEQGLSVFTISIDNSLRAIFGLEDTIRPEAPAVIAELRRRKIEAYIFSGDHKGAVEKVATELGFPNQNYRYGCSPDQKSAEIKSLQEASPGTVLFLGDGTNDAVALTQADVGFSFGDSTEVAVSASDIAVLSTSLNSILEFLDLCIRVKRCILAGFSWAIFWNLFAVLAAAGAFVKFRIEPQWAGLGELGSVLPVVFIAALVGIFWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5K7</accession>
    <name>D5G5K7_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Kinase</fullName>
            <ecNumber evidence="4">2.7.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00004386001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the inositol phosphokinase (IPK) family.</text>
    </comment>
    <dbReference type="EC" id="2.7.-.-" evidence="4"/>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835643.1">
        <property type="nucleotide sequence ID" value="XM_002835597.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5K7"/>
    <dbReference type="FunCoup" id="D5G5K7">
        <property type="interactions" value="57"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G5K7"/>
    <dbReference type="GeneID" id="9183786"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004386001"/>
    <dbReference type="eggNOG" id="KOG1620">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042569_3_1_1"/>
    <dbReference type="InParanoid" id="D5G5K7"/>
    <dbReference type="OMA" id="FRICGMK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016303">
        <property type="term" value="F:1-phosphatidylinositol-3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000827">
        <property type="term" value="F:inositol-1,3,4,5,6-pentakisphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000825">
        <property type="term" value="F:inositol-1,3,4,5-tetrakisphosphate 6-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000824">
        <property type="term" value="F:inositol-1,4,5,6-tetrakisphosphate 3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008440">
        <property type="term" value="F:inositol-1,4,5-trisphosphate 3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000823">
        <property type="term" value="F:inositol-1,4,5-trisphosphate 6-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032958">
        <property type="term" value="P:inositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000821">
        <property type="term" value="P:regulation of arginine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.160">
        <property type="entry name" value="Inositol polyphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005522">
        <property type="entry name" value="IPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038286">
        <property type="entry name" value="IPK_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12400">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12400:SF103">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE MULTIKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03770">
        <property type="entry name" value="IPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="228"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ79800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="295" mass="32536" checksum="E2A87327855006A6" modified="2010-06-15" version="1">MAASGLKASDLQAFNFAAAGHDGVLSDESGALVIKPCTAREVAFYTSASEHPKFQKWMPTFFGTLELSGSEPAAGPVTSNRNTSIVLQSLTYGFCKPCVMDIKLGAQLWDDEAPQEKRDRLDAVSNATTSSSLGLRIAGMKVWKGEAAGGYKVFDKHYGRTFTAENCSQAIEEYLSADISEEQKKLIVGRFLRKVTEVREMLEEQESRMYSASFLFVYEGDREVLGTALEEEEENAKKPPKGEDDDSEEDEKAKKVEELKLIDFAHANWTPGKGPDENALQGVRSTERLLAELVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5U5</accession>
    <name>D5G5U5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00001581001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
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        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Pre-mRNA processing factor 4 (PRP4)-like" evidence="5">
        <location>
            <begin position="66"/>
            <end position="116"/>
        </location>
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        </location>
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        </location>
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        </location>
    </feature>
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        </location>
    </feature>
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        <location>
            <begin position="484"/>
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        </location>
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            <begin position="105"/>
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        </location>
    </feature>
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        <location>
            <begin position="124"/>
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        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAZ79888.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="522" mass="56985" checksum="D645B1A9C9CC0FCA" modified="2010-06-15" version="1">MSGIHPSRRAYVEEDDSDMVDLANVPIDRDYNLPQNLKSGIAPEKASAILSQMDRKKKAAAIVVPTDDGRVRGRLRELGEPMTLFGEGPGDRRDRLRELLYQLQEGNAGEGSQAGDMDMRDATETPEPDEDEEEEFYTPGVPGLLEARQWMANYSLPRAHARIAKQKAEASIALQRHIKHRRAIKERLSGYQVLGSQIAGERTVSIARFSPDSEIIAAGSWSGSIRLLTIPNLEDKSLLRGHTERVGGITWHPQATVSLSPDSANLASGGGEGTVHLWSLTQDTPIATLTGHSARVARVEHHPSGNYLASASYDTTWRLWDLPTQKELLLQEGHSREVYTVAFQIDGALIASGGLDSIGRVWDLRTGRTIMVLDGHIREVTGLAWSPDGYRVVSSSGDATVKVWDVRMVRCTATIGAHKGLVSDVRFFSGKQDSSVAMDGTDEGQAAAAAAEAMRTEGTYLATSGFDGNVNIFSSDDWALVKNLSAHSGNVTSVDISRDDRFIVSSGQDRTVKLWAREDIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5W6</accession>
    <name>D5G5W6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00001603001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430002">
        <property type="protein sequence ID" value="CAZ79909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835752.1">
        <property type="nucleotide sequence ID" value="XM_002835706.1"/>
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    <dbReference type="STRING" id="656061.D5G5W6"/>
    <dbReference type="GeneID" id="9187587"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001603001"/>
    <dbReference type="eggNOG" id="ENOG502SG1C">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NUFIP1_cons_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10453">
        <property type="entry name" value="NUFIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="148"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="148"/>
            <end position="176"/>
        </location>
    </feature>
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        <location>
            <begin position="35"/>
            <end position="152"/>
        </location>
    </feature>
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        <location>
            <begin position="167"/>
            <end position="190"/>
        </location>
    </feature>
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        <location>
            <begin position="40"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
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            <begin position="75"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="105"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="167"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ79909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="230" mass="25710" checksum="02676FD5182DA88D" modified="2010-06-15" version="1">MDEETWLRLNGGKLLGTNLKPPETPEEIEEWIRERRRRFPTRERVAAKVKEEEEKKRKLAGASDASAETVRAQAPATSRQALATAQQPSQQKRKRTEQDPGNDYPSDSSLDSDADTNSQSSFPEELPTSKKPPPPSPTASTPSRKKSTKQAALCRVFRTTGKCRFGEGCRYRHVPPEGKRKKGGERKGAKSLYQRLLDQDREKENEIVVSAVRYLFERGALSVSEAGGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5G663</accession>
    <name>D5G663_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00001791001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430007">
        <property type="protein sequence ID" value="CAZ80006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835849.1">
        <property type="nucleotide sequence ID" value="XM_002835803.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G663"/>
    <dbReference type="STRING" id="656061.D5G663"/>
    <dbReference type="GeneID" id="9187685"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001791001"/>
    <dbReference type="eggNOG" id="KOG0742">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004471_6_5_1"/>
    <dbReference type="InParanoid" id="D5G663"/>
    <dbReference type="OMA" id="ECATSDI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19481">
        <property type="entry name" value="RecA-like_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054289">
        <property type="entry name" value="DUF7025"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46411:SF1">
        <property type="entry name" value="FAMILY ATPASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_7G05752)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46411">
        <property type="entry name" value="FAMILY ATPASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22942">
        <property type="entry name" value="DUF7025"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="2">
        <location>
            <begin position="764"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="212"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="601"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="17"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="164"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ80006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="986" mass="110311" checksum="7618F77D1338A611" modified="2010-06-15" version="1">MTSKPTSPAEEVSANETTPSETSVTSTASTATPTPTSTDGSATPSTSHDSAPGTATASQSTTTVAAPENAAAKTKKPMCKHKKLKAEEKEKEKEKERAKKEQEEKEKAEREKDEREKKEKEEKEKEDKGKEDKKGRGKDKKKDKKKLRSKKSKKRSRKSDSESSDSSSSSDSSCASDDSDSDSSSSSSSSSDSSSDSELENEALAAAVRLILARKSSKRRRREKLKAKKKAKARKEKKKAKKAEREAAAAMMGEGGENPPEPGEEEGDGKERASVLEYKRVDELWDKNIHDYTITDTVAHTKTDKYSSFIFTVRRVFDYENKYVETMVDIKSILLKEALTEIIGDVRGVSLVEDVPEVDPNMLFNFLPEMEAWPEDIPLTPEEISQQIDQVKLLIGYLNTDYAGVKATLYPLLANSMITFDLLWALLKPNTMMYTTCAGSNEPRAFKLEYAQKESSFMRGKWWGIEGKYLEYAGKDSKPSEANKEVGGFGWGTIMVDIDGFKGARKISSLSCYPLEYRKDKDTMRETLVERGRKFVSLRGMQYKMHKGLAFMKKKRQYAKVHVKGRIMIDPATFRRINPNYLISSVKSRNNDEEDDNQVNFFDAEGQNGSSPSSETGDCTDCCCGGEGEEEEEGENEIPAETEKHKIVTDGKGAFYVVTEQEANDKSMRVEALPEDGDEEAEPVFTDDELLTASPVVLGWSFNEKLWLEFTVSGVEEIQWNEKAFDSLVLAEEQKEIVKALVESHSGNNIASATIDDVIQGKGRGLVSVLHGPPGVGKTLTAEGIAELLKKPLYCVSSGELGTNPAVLEHELNKILDIAHSWGAVLLLDEADVFLERRTMADMHRNALVSIFLRLLEYFQGILFLTTNRVETFDDAFQSRIHVALRYNELTFKAKVKIWKMFLEMVRVKYGEDAPEVLSQEEIEWLAKKHLNGRQIKNSVRTAQALANNKKERLQMKHIRTVLNVAEGFENDLKGTGQLDSMHAYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6B3</accession>
    <name>D5G6B3_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">GSTUM_00004402001</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="FN430009">
        <property type="protein sequence ID" value="CAZ80056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835899.1">
        <property type="nucleotide sequence ID" value="XM_002835853.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6B3"/>
    <dbReference type="FunCoup" id="D5G6B3">
        <property type="interactions" value="1128"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G6B3"/>
    <dbReference type="GeneID" id="9187427"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004402001"/>
    <dbReference type="eggNOG" id="KOG0331">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_16_9_1"/>
    <dbReference type="InParanoid" id="D5G6B3"/>
    <dbReference type="OMA" id="STMPKFE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051880">
        <property type="term" value="F:G-quadruplex DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002151">
        <property type="term" value="F:G-quadruplex RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071042">
        <property type="term" value="P:nuclear polyadenylation-dependent mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17966">
        <property type="entry name" value="DEADc_DDX5_DDX17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000008">
        <property type="entry name" value="ATP-dependent RNA helicase RhlB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000079">
        <property type="entry name" value="probable ATP-dependent RNA helicase DDX17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 10" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="14">
        <location>
            <begin position="126"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="157"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="360"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="504"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="9">
        <location>
            <begin position="126"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="510"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAZ80056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="547" mass="58870" checksum="EC534DF6BC89C124" modified="2010-06-15" version="1">MAYDAYRGSGNSYGGGGGYGGGGGGYGGGSNGYSNGGPRGGAGSYGSGNSYGSGGYGGGGAGGDRMSNLGAGLKAQSWDMNSLPKFEKSFYKEAPTVASRTPAEIEAFRLEKQMRVQGRDVPKPVVDFDEAGFPSYVMNEVKAQGFAAPTAIQSQGWPMALSGRDVVGIAETGSGKTLTYCLPAIVHINAQPLLAPGDGPIVLVLAPTRELAVQIQQEVAKFGKSSRIRNTCVYGGVPKGPQVRDLSRGVEVLIATPGRLIDMLETNKTNLRRVTYLVLDEADRMLDMGFEPQIRKILGQIRPDRQTCMWSATWPKEVRQLASDFLNDFIQVNIGSLELSANHNIQQIVEVINDYDKRDRLIKHLEKVMDDKNSKCLIFTGTKRTADDITRFLRQDGWPCLAIHGDKQQTERDWVLNEFKTGKSPIMVATDVASRGIDVRNITHVINFDYPNNSEDYVHRIGRTGRGGARGTAITFFTTNDAKQARDLLTVLREAKQQIPPELADMSRSGFGGGGGRYGSSRGRGGRGGGGGGFTSSNSAPLGRSRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6B8</accession>
    <name>D5G6B8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00004413001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430009">
        <property type="protein sequence ID" value="CAZ80061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835904.1">
        <property type="nucleotide sequence ID" value="XM_002835858.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6B8"/>
    <dbReference type="GeneID" id="9187432"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004413001"/>
    <dbReference type="HOGENOM" id="CLU_2706591_0_0_1"/>
    <dbReference type="InParanoid" id="D5G6B8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ80061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="73" mass="8651" checksum="4BDA2839BB56D118" modified="2010-06-15" version="1">MQIRWERFQIIFNIVSAPPPWCLARHLELWVWALYTLQDQKDSRHQLMETTNHVGYTIQSSSPATMTINLVHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6D3</accession>
    <name>D5G6D3_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 3 subunit L</fullName>
            <shortName evidence="4">eIF3l</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00004430001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the eIF-3 subunit L family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430009">
        <property type="protein sequence ID" value="CAZ80076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835919.1">
        <property type="nucleotide sequence ID" value="XM_002835873.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6D3"/>
    <dbReference type="STRING" id="656061.D5G6D3"/>
    <dbReference type="GeneID" id="9181161"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004430001"/>
    <dbReference type="eggNOG" id="KOG3677">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029210_2_0_1"/>
    <dbReference type="InParanoid" id="D5G6D3"/>
    <dbReference type="OMA" id="AGWFIRN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03011">
        <property type="entry name" value="eIF3l"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019382">
        <property type="entry name" value="eIF3l"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13242">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13242:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10255">
        <property type="entry name" value="Paf67"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="198"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="232"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="PCI" evidence="7">
        <location>
            <begin position="260"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ80076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="478" mass="54675" checksum="EC8C3E9C9C64F088" modified="2010-06-15" version="1">MLAGQNFNGTNRHLREDSDTDDEVLASTLVEEALRDDGDYDDLESAGGSLQISGENIQQRLAAAATPLDFQAPLQNRFDSYDNYCTLFHFILNSDGPVDLELPTYYWAWDVIDEFIYQFNSFCSHRNAVARKEGGGSEEERRLLCDHPQTWGCYSVLNVLYSLIQRSQMNEQLAAIKKGQDPNEVAGEYGSRPLYRMLGYFSIIGLLRVHCLLGDFTLALKTLDDIELNKKAVFARVMAAHFTTYYYVGVSYMMMRRYADAIKAFSHILVYVSRTKNFQKNAQFDSVTKKNDQMYALIAICVALCPTRLDDTIHASLREKYGEQFTKMQRGGPDALQIYEQLFMSACPKFISPIPPDFDKPENNVDPSQHHLRVFMADVRNSMLAPTLKSYLKLYTTMDLKKLAAFIEVDPEVLRTQLLVYKQRSRQTRWIEGGLLEGETINTSDLDFALQGDLIHISEAKVGRRLVDWYLRNLAKTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6S5</accession>
    <name>D5G6S5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00002247001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430014">
        <property type="protein sequence ID" value="CAZ80218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836061.1">
        <property type="nucleotide sequence ID" value="XM_002836015.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6S5"/>
    <dbReference type="STRING" id="656061.D5G6S5"/>
    <dbReference type="GeneID" id="9187243"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002247001"/>
    <dbReference type="eggNOG" id="KOG4400">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058879_2_0_1"/>
    <dbReference type="InParanoid" id="D5G6S5"/>
    <dbReference type="OMA" id="KGNPTCY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051714">
        <property type="entry name" value="Znf_CCHC_NABP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23002">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="2">
        <location>
            <begin position="12"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="2">
        <location>
            <begin position="55"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="2">
        <location>
            <begin position="91"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="2">
        <location>
            <begin position="124"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="2">
        <location>
            <begin position="142"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="2">
        <location>
            <begin position="164"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ80218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="183" mass="19226" checksum="7AFF8AE6F7CA3C77" modified="2010-06-15" version="1">MDFGSGNNARACYNCGEGGHQARECPKRGTPVCYNCGRMFFPRRDCTGPAKEKSCYRCGQTGHLSRDCNDAPQQVGSFGGGSYGSGGGAECYKCGKVGHIARQCTASGAGYGGPPGGGARQQTCYSCGGYGHLSRDCTQGQKCYNCGQIGHLSRDCPSEQDRVCYKCKQPGHVMASCPEAQAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5G760</accession>
    <name>D5G760_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00002355001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430021">
        <property type="protein sequence ID" value="CAZ80353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836162.1">
        <property type="nucleotide sequence ID" value="XM_002836116.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G760"/>
    <dbReference type="GeneID" id="9185251"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002355001"/>
    <dbReference type="HOGENOM" id="CLU_2335166_0_0_1"/>
    <dbReference type="InParanoid" id="D5G760"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="98" mass="11528" checksum="7280DE63FB5D223A" modified="2010-06-15" version="1">MGSYGMASLPGIKFYRARYHTLFLLLLGMLYSTGVIQWDNVLIARTLEMEVLFWDPAPYDTMIRNWLAGWLEKLRKHYPPYDIVQPVFEDHEMVENGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7B4</accession>
    <name>D5G7B4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00002427001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430023">
        <property type="protein sequence ID" value="CAZ80407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836216.1">
        <property type="nucleotide sequence ID" value="XM_002836170.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7B4"/>
    <dbReference type="STRING" id="656061.D5G7B4"/>
    <dbReference type="GeneID" id="9185305"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002427001"/>
    <dbReference type="eggNOG" id="ENOG502QURC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_776564_0_0_1"/>
    <dbReference type="InParanoid" id="D5G7B4"/>
    <dbReference type="OMA" id="MAIIRND"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007541">
        <property type="entry name" value="Uncharacterised_BSP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33321">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33321:SF12">
        <property type="entry name" value="PLANT BASIC SECRETORY PROTEIN (BSP) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04450">
        <property type="entry name" value="BSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="273"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="273"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="336"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="357" mass="39514" checksum="4FB82AC621AC9526" modified="2010-06-15" version="1">MAATTTRQPSLLVQVSAALSRPASPTAGAPTRKPHHPKHWHIPRLLFMVKDVSHEGATKYFNTTNTQKLLHDAVISVLETLYTHETAPTTQRSITLILQSMDGIANTTGNELDNDCKEIYFSLDYIGQLSDDRTQNEILGVVRHEMVHCWQYDACGTAPGGLIEGISDFVRMKARLGPPHWTRRGDRWDAGYETTAYFLDYLCSRFGNDTVKRVNLSMKHSKYTPELWMTISGGIPVEKLWDDYKTAFNLHPTPKPEPGDDEEAKEDYVVIAKEEATTSPPRKTEAKNPQSVGEPLPVPLTPTSTHAFSMCSPGSPGPGDARPFAAGTPAAQAMLDRSRDTTSEEKPDATRLIDITP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7N5</accession>
    <name>D5G7N5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00004647001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430031">
        <property type="protein sequence ID" value="CAZ80528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836337.1">
        <property type="nucleotide sequence ID" value="XM_002836291.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7N5"/>
    <dbReference type="FunCoup" id="D5G7N5">
        <property type="interactions" value="96"/>
    </dbReference>
    <dbReference type="GeneID" id="9187007"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004647001"/>
    <dbReference type="eggNOG" id="KOG1289">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004495_2_4_1"/>
    <dbReference type="InParanoid" id="D5G7N5"/>
    <dbReference type="OMA" id="TYCIAMF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015101">
        <property type="term" value="F:organic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000046">
        <property type="entry name" value="Amino-acid permease, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649">
        <property type="entry name" value="AMINO-ACID PERMEASE BAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649:SF7">
        <property type="entry name" value="CHOLINE TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="196"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="229"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="274"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="318"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="344"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="424"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="531"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="555"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="591"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="628"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="64"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="80"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ80528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="677" mass="74247" checksum="82A426C313D2911D" modified="2010-06-15" version="1">MELESLIINPAAQGTSVRKKKIEKTRIASCGSLSPKYNSRPRQEGERKGAGFYLPIASFDPLEANDSNIANESYQEEGGAEAHESYRHRNSDTDDSDDSDQEAGHHDTQYPQHFLPSTPAIIKSSLIHFLSIIPNSIFPSNEMTTVPSYAQGEEDTTLKDKNVTYLDGDVGSMDSDTVKLAKMGYTQDLERNFSKWSLLGVSFALTNSWFGISASLVTGINSGGPVVTVYGIILIALINGCAGISLAELVSAMPNSGGQYYWVSVLAPPRHMKFLSYLTGAIGYAGSIFTCASVALAIGSALMGMIQMNHPDLVIERWMVFVSYQIFNLFAWVFNCYGKTLPKVASFFLYVSLTSFIVITITVLATSSPKQNAKFVFANFVNNTGWESNAIAFIVGLINPNWSFACLDSATHLAEEVPRPESNIPFAIIGTVAIGFVTAFLYSIAMFFSMTNLDELVKTATLVPILELYRQATGSKPAAIFLEFLICFTGLGCQIACHTWQARLCWSFARDKGLPGSRYWSQVHPTMGIPFYAHTMSCIVVALLGLLYIGSTTAFNSMVTACIVLLYISYAIPVILLLMKGRNNIKHGPFWVGKLGLVANLVLLFWTGFTLIMYSFPYSMPVTSGTMNYVSAVYGFVFILTIGYWFARGKRTFRGGGERAAEAEHIVREVASHAHAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7P9</accession>
    <name>D5G7P9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">FACT complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">GSTUM_00004673001</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 11">Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the FACT complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the peptidase M24 family. SPT16 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430031">
        <property type="protein sequence ID" value="CAZ80542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836351.1">
        <property type="nucleotide sequence ID" value="XM_002836305.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7P9"/>
    <dbReference type="FunCoup" id="D5G7P9">
        <property type="interactions" value="1251"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G7P9"/>
    <dbReference type="GeneID" id="9187021"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004673001"/>
    <dbReference type="eggNOG" id="KOG1189">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004627_1_0_1"/>
    <dbReference type="InParanoid" id="D5G7P9"/>
    <dbReference type="OMA" id="YHINTIP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035101">
        <property type="term" value="C:FACT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034728">
        <property type="term" value="P:nucleosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01091">
        <property type="entry name" value="CDC68-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.150:FF:000002">
        <property type="entry name" value="FACT complex subunit SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.210:FF:000001">
        <property type="entry name" value="FACT complex subunit spt16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000017">
        <property type="entry name" value="FACT complex subunit SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.350.10:FF:000006">
        <property type="entry name" value="FACT complex subunit SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.230.10:FF:000005">
        <property type="entry name" value="FACT complex subunit spt16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.210">
        <property type="entry name" value="FACT complex subunit Spt16p/Cdc68p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029148">
        <property type="entry name" value="FACT-SPT16_Nlobe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056595">
        <property type="entry name" value="Fact-SPT16_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048969">
        <property type="entry name" value="FACT_SPT16_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013953">
        <property type="entry name" value="FACT_SPT16_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013719">
        <property type="entry name" value="RTT106/SPT16-like_middle_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040258">
        <property type="entry name" value="Spt16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033825">
        <property type="entry name" value="Spt16_M24"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13980">
        <property type="entry name" value="CDC68 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13980:SF15">
        <property type="entry name" value="FACT COMPLEX SUBUNIT SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14826">
        <property type="entry name" value="FACT-Spt16_Nlob"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24824">
        <property type="entry name" value="PH_SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08512">
        <property type="entry name" value="Rttp106-like_middle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08644">
        <property type="entry name" value="SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21091">
        <property type="entry name" value="SPT16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01285">
        <property type="entry name" value="FACT-Spt16_Nlob"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01287">
        <property type="entry name" value="Rtt106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01286">
        <property type="entry name" value="SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 11" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8 11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 11" id="KW-0235">DNA replication</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 11" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="FACT complex subunit SPT16 N-terminal lobe" evidence="13">
        <location>
            <begin position="7"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="FACT complex subunit SPT16 middle" evidence="14">
        <location>
            <begin position="538"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="domain" description="Histone chaperone RTT106/FACT complex subunit SPT16-like middle" evidence="15">
        <location>
            <begin position="814"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="440"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="935"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="440"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="468"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="937"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="996"/>
            <end position="1013"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM01287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAZ80542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1019" mass="115371" checksum="973F223EF0AC132A" modified="2010-06-15" version="1">MGDEVSIDKSLFHSRLGGLVAAWKDSKKADLFGGVGSIVIILGKTVEGPYSKSLALHFWLLGYEFPTTLFVVTQEKFYVVTTPKKSKHLETLKGGKFPIEILVRGKDEAQNTQNFKDLAEVIKKSGKKVGVCMKDKAEGPFVNDWKKIFPAEIEGIEEFDVSPAISQCLAIKDDLELKTMRASSRALVGIMKDYFIDEMSTIIDEEKKITHMQLSQKIEAKIDDEKFFRAKEMKLGPDFDPLQLDWTVGPLVQSGGRYDLRSSATSDDSQLHGGIILSTMGLRYKSYCSAIARTFLIDPNKSQEKYYSFLVDLQWKVLSEIRDGVVCKDVYNKAIAFIKSKHPELEKHFLKAIGSIIGIEAKDTTTPITAKSNRVVKDGMTLCVTVGFADLENPKPQDSKSKVYSLVLTDTIKVTPGDPIVFTGGCSKDLKETAFYFKDEEPEQKVKEKKPAPKPAPAKNTAVLKAKLRGERKEVDEGAEQKRREHQKELAQQKQEEGLARYAEGDAVGDGKGKKAIKRFESYKRENQLPLSVADLKIVVDAKSQTIIVPVFGRPVPFHIATLKNASKTDEGDWTYLRINFLSPGQGVGRKDDLPFEDPNAHFVRSLTYRSTDNDRMAEICAAIQDMKKNAVKREQERKEMEDVVTQDNLVEIRNRRPQRLGDIYVRPALEGKRVPGEVEIHQNGLRYQSPVRNDHRIDVLFSNVKHLFFQPCAHELIVLIHVHLKDPIMVGKKKTKDVQFYREATDIQFDETGNRKRKYRYGDEEEFEQEQEERRRRALLDKEFKAFADKISEAGKKHENGVDVDIPYRELGFNGVPFRANVLCAPTTDALVQLTDPPFLVITLDEIEIAHLERVQFGLKNFDLVFVYKDYTRPVSHINSIPMESLENVKEWLDSSNIPFTEGPLNLNWPTIMKTVIADPHQFFKDGGWNFLSVESDADDDDEEDEVSEFEADSSDSESEGSEEDSEFDDGDASADEGSEASDDESGDDWDDLENEAKRKDKEVSVKVEEKPKKGKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8A3</accession>
    <name>D5G8A3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00002948001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430041">
        <property type="protein sequence ID" value="CAZ80746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836555.1">
        <property type="nucleotide sequence ID" value="XM_002836509.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8A3"/>
    <dbReference type="FunCoup" id="D5G8A3">
        <property type="interactions" value="110"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G8A3"/>
    <dbReference type="GeneID" id="9188348"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002948001"/>
    <dbReference type="eggNOG" id="KOG1347">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012893_1_2_1"/>
    <dbReference type="InParanoid" id="D5G8A3"/>
    <dbReference type="OMA" id="AAWFELF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13132">
        <property type="entry name" value="MATE_eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045069">
        <property type="entry name" value="MATE_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="120"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="168"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="206"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="236"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="282"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="403"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="475"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="502"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ80746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="546" mass="58968" checksum="8260B00407A0A43D" modified="2010-06-15" version="1">MGREEQDLLKGSKVISSDYGTLATSARQALSSSPSWSTTQDGGSLETTLTTETTGLLGGHPNNISKWDEAAAAGTVYTSYTREARMLVKCTAPLWLTFLLQYSLTFASLFSAGTLGKNELAAVTLAGMTANITGYAVFQGLSTSLDTLCAQAYGSGNKALVGLHLQRMIWFLGLVSVPIITAWCFAEPILHAMVPEPELAVLSGRYLRILTIGAPGFALFEAGKRFTQAQGKHPDPFYFIFLFHICIFAASTFVLLICAPVNAVLNYLLVWHPTIGVGFNGAPFAVVIVDWLMPILLFCYVYFIDGRECWTGFTKRGLQNWWPMIKLSLPGLAMIEAEYLAFELLTLMSARFGTSYLAAQSIISTGATITYQLGFALSIASSTRVANFLGAAMGDAAKVSARTGFVVAIAVGIFNCCAMLSVRKQFAKLFTANAEIISLVVRVMPLTAAFQPHDALACLGSGLLRGQGRQHIGSYINIIGYYGIAMPVSVICAFVLGWKLMGLWCGVVVALAFVSSLQSFIILRTNWDKVRTLPSHTLSSDSKVLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8B2</accession>
    <name>D5G8B2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00002960001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the proteasome-dependent degradation of fructose-1,6-bisphosphatase.</text>
    </comment>
    <dbReference type="EMBL" id="FN430041">
        <property type="protein sequence ID" value="CAZ80755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836564.1">
        <property type="nucleotide sequence ID" value="XM_002836518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8B2"/>
    <dbReference type="STRING" id="656061.D5G8B2"/>
    <dbReference type="GeneID" id="9188357"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002960001"/>
    <dbReference type="eggNOG" id="KOG2659">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_802127_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8B2"/>
    <dbReference type="OMA" id="HIIEMIR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013144">
        <property type="entry name" value="CRA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024964">
        <property type="entry name" value="CTLH/CRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006595">
        <property type="entry name" value="CTLH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050618">
        <property type="entry name" value="Ubq-SigPath_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12864">
        <property type="entry name" value="RAN BINDING PROTEIN 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10607">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08513">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00757">
        <property type="entry name" value="CRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00668">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50897">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CTLH" evidence="2">
        <location>
            <begin position="169"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ80755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="346" mass="38803" checksum="26592E6E8B098766" modified="2010-06-15" version="1">MRDVLGAVHILFRLIVNQMVKTISFPFPLNFFFLTDALPIPPPASTGTLSHKHANSYIITTNPTLPFYPFLSFFPPLSSLPLLPLFPFPQHPPVWVPLRYPVNMSHSTTTSSRKAPGAADWEKKVDEVKVSKADLNTLVMNYLIIEGYKSAAVNFAQEANMSPQVDLDSIQERVDIRHAIHHGDIQTAIERINELHPDLLETNLPLHFSLLRLQLIELIRNCTQSPDGDISEALAFATTHLAPRAPGNSKFLQDLERTMALLCFPMENLAPPLAELMDPALRRQVAAKVNEAILEVQGVPKEAKIRRLVRLRAWAEQRMRDGHREVPNMDLGLDVTSQTSHDAMGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8E5</accession>
    <name>D5G8E5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phosphoserine phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">O-phosphoserine phosphohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00004781001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HAD-like hydrolase superfamily. SerB family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.3" evidence="4"/>
    <dbReference type="EMBL" id="FN430042">
        <property type="protein sequence ID" value="CAZ80788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836597.1">
        <property type="nucleotide sequence ID" value="XM_002836551.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8E5"/>
    <dbReference type="FunCoup" id="D5G8E5">
        <property type="interactions" value="516"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G8E5"/>
    <dbReference type="GeneID" id="9186008"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004781001"/>
    <dbReference type="eggNOG" id="KOG1615">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036368_5_1_1"/>
    <dbReference type="InParanoid" id="D5G8E5"/>
    <dbReference type="OMA" id="EWNVDVI"/>
    <dbReference type="UniPathway" id="UPA00135">
        <property type="reaction ID" value="UER00198"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036424">
        <property type="term" value="F:L-phosphoserine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07500">
        <property type="entry name" value="HAD_PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000143">
        <property type="entry name" value="Phosphoserine phosphatase serb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050582">
        <property type="entry name" value="HAD-like_SerB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004469">
        <property type="entry name" value="PSP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01488">
        <property type="entry name" value="HAD-SF-IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00338">
        <property type="entry name" value="serB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43344">
        <property type="entry name" value="PHOSPHOSERINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43344:SF2">
        <property type="entry name" value="PHOSPHOSERINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01137">
        <property type="entry name" value="C1.6.1:_Phosphoserine_Phosphat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01136">
        <property type="entry name" value="C1.6:_Phosphoserine_Phosphatas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00029">
        <property type="entry name" value="phosphoserine_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0718">Serine biosynthesis</keyword>
    <feature type="active site" description="Nucleophile" evidence="11">
        <location>
            <position position="204"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR604469-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ80788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="420" mass="46640" checksum="7C25D2A4F15AD3C2" modified="2010-06-15" version="1">MSRTEPSNISPPAPRQGLARAASYNAALHIRDLHGAPPTPNEDLTTPVEIITPEGKRVLIKNSGIVHTVSHKNCVLTESALQLVATLFYNPNQSLITVDGEEINVEKQYVNFGPYISDMAMFEFIQTIDELHVPWKKWGTNHRCLDSAEHPKVMEITLSMPEMLHLSELRRHTSLWKFERKWNVELVLQENDIFRRHKRLAVFDMDSTLIEQEVIDEIAKFIGVEDQVSSITARAMNGEIDFTESLRQRAALLKGVPSTVFQQLRSSITFTPGVRELCRALKTLGYKLAVLSGGFIPLAEYVKGQLDFDYAHANNLVVSEDGKFLTGDLSGPIVHAERKALLLEEIAKENGIALEQTMAIGDGANDLLMMKKAGLGIAFNAKPSVQVAAPARLNSETMLDVLYILGFTKEEIQALLAVAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8E7</accession>
    <name>D5G8E7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00004784001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430042">
        <property type="protein sequence ID" value="CAZ80790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836599.1">
        <property type="nucleotide sequence ID" value="XM_002836553.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8E7"/>
    <dbReference type="GeneID" id="9186010"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004784001"/>
    <dbReference type="eggNOG" id="ENOG502T1H8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_272163_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8E7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="220"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="384"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="474"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="545"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="221"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="389"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="516"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="564"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="622"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="859"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="877"/>
            <end position="888"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1188" mass="129735" checksum="1995BFE5BA9DF9E4" modified="2010-06-15" version="1">MNRSSTVDASKSPSSGAGQDTNGGAQLREDIILTPASTTMSEGSEGERNERREAEGGLTRPTEDNAEMGEPKMDNNNNRRDNIPEVEDWAQYTPSRNRDIREEMGSLNNEIPNLKELFARTSSAMDEEADHLTRVKANHRSSLASLAESHRTEISNLTASHQNEIKKLEESHVEEMRNARRGYGFNNDLVRGLHESYGKQIESLVLDLGEARRQLKMKLRSISSTSSSPMATKSSVSSFPDEDPLVHDILARRLPMILNFGDLDKALSTTEISIPHPGSSFRAVYKHLCHAAKNHLQALNAAAELFATGAKWTSEVRKIKHWYQSALEERIHFRILNESGGSNIKDLWETGLWKADDFILQVDCAAEEAWPKVHVYDEGIAEQRKKLTSPSGGRPSSLRSQSRTSFGLTSEQLIEVEGETSRGFDATAELERINRELGELEVHDHLRHAGDENKENIDGSVPKRYVTVEEVVDEDDPVRAAGEGAEDEDVGLSVPSSPTPTPKEPGETTVPLGWMDNNNIGDKMGSSSRAASNRSLFSKFLEEGLGTGKRDKRPTSGSNASDSWRFDDLFSSPTERKKGKERSTVSSDESLQDRDEQTNGARGPQPWRWILTPGGTVITPPQYDSDNQAPKVGTQSFERASAPRLGRLNEEDTEDEHIISESDQPDVEQEELRTERKRRKPFFLFGEGPAASPAPPPAPEPSTRKSPTLPAYLRAEMESSYQSTRIDDDSDIDDNQPREADIENAFSKMKPGPCDTPVMGNSRTAVSLTPLLGDRSPTPRRGGPSLRLDTDNFKATRGPGERSAPMPKAPNPSASTKGPKYRGGSGSYGYPGPSHVQEGGGLDEPWESASEGSASGKWGSRRGNRCGHGKGPSTRTWRRDLDSEDFRSRSVSPQIPPPSRSSMTTTDRSADFIPPPQTYQQNIGSGSGSNGPVRYGGVRYMGPLGPTHMRNYAGAHQADMIFHQQMKNPKELFAAGYRPNGPPPFISPMGPVYAPNPPHSVSAFGGGAMGRSPAFLLGNNEQRVASENGGNWPMRPPLTAHPQFGPHMWNLSGEGPRRSYSTDSISPHLWKLDAEGGLQQAPGPIPGPTMPPQRPGSTIADQTAYQNQINASLMSALANGYAQQPSTPGSHGSVVGQMPGPQWYLNMQQAQAQAQMQGNPTIPPGFEAWTSSPIGGMPGQWAIGPFSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8Q8</accession>
    <name>D5G8Q8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Clustered mitochondria protein homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Protein TIF31 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">CLU1</name>
        <name evidence="3" type="synonym">TIF31</name>
        <name evidence="6" type="ORF">GSTUM_00003100001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">mRNA-binding protein involved in proper cytoplasmic distribution of mitochondria.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">May associate with the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CLU family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430050">
        <property type="protein sequence ID" value="CAZ80901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836710.1">
        <property type="nucleotide sequence ID" value="XM_002836664.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5G8Q8">
        <property type="interactions" value="913"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G8Q8"/>
    <dbReference type="GeneID" id="9186269"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003100001"/>
    <dbReference type="eggNOG" id="KOG1839">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003256_2_0_1"/>
    <dbReference type="InParanoid" id="D5G8Q8"/>
    <dbReference type="OMA" id="HPVWDKD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048312">
        <property type="term" value="P:intracellular distribution of mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd15466">
        <property type="entry name" value="CLU-central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000293">
        <property type="entry name" value="Clustered mitochondria protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2280.10:FF:000002">
        <property type="entry name" value="Clustered mitochondria protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2280.10">
        <property type="entry name" value="Hypothetical protein (hspc210)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03013">
        <property type="entry name" value="CLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033646">
        <property type="entry name" value="CLU-central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025697">
        <property type="entry name" value="CLU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028275">
        <property type="entry name" value="CLU_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027523">
        <property type="entry name" value="CLU_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007967">
        <property type="entry name" value="GSKIP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023231">
        <property type="entry name" value="GSKIP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12601:SF6">
        <property type="entry name" value="CLUSTERED MITOCHONDRIA PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12601">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT EIF-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13236">
        <property type="entry name" value="CLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15044">
        <property type="entry name" value="CLU_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12807">
        <property type="entry name" value="eIF3_p135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05303">
        <property type="entry name" value="GSKIP_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13374">
        <property type="entry name" value="TPR_10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103107">
        <property type="entry name" value="Hypothetical protein c14orf129, hspc210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51823">
        <property type="entry name" value="CLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="Clu" evidence="5">
        <location>
            <begin position="332"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="626"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="908"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1216"/>
            <end position="1296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="17"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="626"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="650"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1220"/>
            <end position="1232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1253"/>
            <end position="1263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1274"/>
            <end position="1287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ80901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1296" mass="143814" checksum="DEC0E3CD5742F293" modified="2010-06-15" version="1">MANISEKIRTGDEALDANTSKVTSQPTTERNEREEIFQDGAGAPQLHDLFQLTVKLPHHPNSMQIMVSTSEQVQDIRQSIIELPDTFQYTCFHLEHNGTRISDFVELSDVPGITPDSELILVEDPYTEKDARLHLLRVRELIGAAGPRIDLVNGISAGLCLFDGIGVENRDDSKKPITEEPQPMAGFDFNAPASLDQFLPKFPVHPAPKCVKSIALSPWNPPPHHLRQRGHLIYIQVTTLEGEQFHITSHVSGFYVNRSSSNKFDPAPKLAPKDFSAHSLIALLGLISPSFNKEFHALQEWNAKKEALVTFAPTNAIPAAPWLVPGPDTKYYNHQSDVARPQETYLIAGTENVDSLRDFNEEFQSTRELPRETVQERVFRERLLSKLFADYTDAAVRGVVLIAKGEVAPLNPTEGRDAQIYVYNNIFFSYGADGVGTFSSEGGDEAARVATGKDVMGVKYVNGLDIPGLSTPGTVVVDYLGRRLVAQSIVPGIFRPREEGQSQIDYGGVEGKDVVATTESFVPLFEIMSRAMRVKKHNVWDKEGKKHELVASVETKGLLGTDGRKYVLDLYRTTPLDVDFLEKHWKGTEGEESEKGYPHRMAVLRPELVESFWKTKMREYVSAEMEKRKVAKGEDQKKSDEAKAYAGEEGEAKGEATEKFEEDVQEKKLEEPVNSIDISSFEFSLNPDVLSGQKPETEEEKVQMEKDEKDVRDACNFLLTTAIPRLISDLAEGEVGSPMDGHSLSCLMHKRGVNVRYLGVIASLADEEGTKLGSIKTLAIQEMVSRACKHILNAMLRGLPGPLVPFCVSHFLNCLLGAEHNPNPKVIPDEGLWKLYHDADFGFEKLTIEGLRASIEKEVSKRYRYSLQEDWISGIKHLQMLREIALKFGLQMKIKNYEFGAPKPTSVAAKVNSEASPSTNGNSKGRSSSKKKSPVTSIHEAPIPKRPSITFDPEDIMNVVPVVKDSSSKSLLAEEALEAGRMSLIQDHKEMGQELLLESLSLHEQIYGVLHPEVARVYNTLSMLYYQLEEKTAAVELARKAVIVSERTLGIDSAETLLNYLNLGLFEHANGNTKTALAYIKHALGFWRIIYGQAHPDSVTTMNNVAVMLQALKHYTDSRKWFEASLAVCESIFGKQSPHTATLYFQLAQALALDHDSKGAVNRMRDAYNVFHAKLGPNDRNTKEAESWLDQLTQNAVILAKQAKDIQERKKRGFPMTPHVTMSTRPQPQVGQGSVAPHGVAGSATSASGLGRMDPRSIDELMKFIEGSSEPVAPKKKPTRTNPKRRGGAAGPGPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>D5G901</accession>
    <name>D5G901_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00004919001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430053">
        <property type="protein sequence ID" value="CAZ80994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836803.1">
        <property type="nucleotide sequence ID" value="XM_002836757.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G901"/>
    <dbReference type="GeneID" id="9181277"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004919001"/>
    <dbReference type="HOGENOM" id="CLU_100306_1_0_1"/>
    <dbReference type="InParanoid" id="D5G901"/>
    <dbReference type="OMA" id="FAGQHTQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40462">
        <property type="entry name" value="CHROMOSOME 1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40462:SF1">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ80994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="67" mass="7261" checksum="7A945A4E15C9AE0F" modified="2010-06-15" version="1">MIGGGPESEKKEDGLDKAIDYVQEKFLGQGPQENESALEQAKDGQIAGFIRSQYKGSTGKEFPIADK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5G908</accession>
    <name>D5G908_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00004930001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430053">
        <property type="protein sequence ID" value="CAZ81001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836810.1">
        <property type="nucleotide sequence ID" value="XM_002836764.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G908"/>
    <dbReference type="STRING" id="656061.D5G908"/>
    <dbReference type="GeneID" id="9181284"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004930001"/>
    <dbReference type="eggNOG" id="KOG1840">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_125_15_1"/>
    <dbReference type="InParanoid" id="D5G908"/>
    <dbReference type="OMA" id="KVAIRWN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053137">
        <property type="entry name" value="NLR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46082:SF6">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46082">
        <property type="entry name" value="ATP/GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13374">
        <property type="entry name" value="TPR_10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ81001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="383" mass="42830" checksum="80B86EEFDAB01CAC" modified="2010-06-15" version="1">MCRRARSGRERALGLLHRDTLLCVNNLGVALSNQGKYYEAEEIMYRHALEAVEKALGPDHPVTLDTMNMLGETLLDLRMYDQAEAVCRRALAGREKVLGADHGSTLTSADNLGRALHNQRKCGEAEMMFRYAQVGREKALGPYDVDTILSLHGLAVALLDQKKFEEAVITYQHALGSMERALGPNHPFTLESINMLGEMLLDLKRYEEAEVMCRRALAGRERALGLAHRDTLLSVNNLGVALCNQRKHDEAEAMYRRAFVGAEETLGLDHRDTLLCIYNLAGALYKQGKYYEAEMMYWRVLAGRERVLGPGHPYTLRCIVKLVAVLYDQGKFSGAETMYRRAVTEGEQALGPRYRTALKNANCLAMFLRARGSSSTGESSLPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5G969</accession>
    <name>D5G969_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00003175001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430056">
        <property type="protein sequence ID" value="CAZ81062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836871.1">
        <property type="nucleotide sequence ID" value="XM_002836825.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G969"/>
    <dbReference type="GeneID" id="9184348"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003175001"/>
    <dbReference type="HOGENOM" id="CLU_2387760_0_0_1"/>
    <dbReference type="InParanoid" id="D5G969"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ81062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="94" mass="10609" checksum="54574E06167942F5" modified="2010-06-15" version="1">MILVLVSARLLVWATSTRRSSSNPQSLIFLVASVTRLLADIIPVSYCGSRLPEGRTSSSPCRRVRIVGYSTYHWLRLLCSTVIRRWLDLCWGLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9B7</accession>
    <name>D5G9B7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="11">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00003300001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="7">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="7">Divalent metal cations. Prefers magnesium or manganese.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the malic enzymes family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430058">
        <property type="protein sequence ID" value="CAZ81110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836919.1">
        <property type="nucleotide sequence ID" value="XM_002836873.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9B7"/>
    <dbReference type="STRING" id="656061.D5G9B7"/>
    <dbReference type="GeneID" id="9182861"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003300001"/>
    <dbReference type="eggNOG" id="KOG1257">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011405_5_2_1"/>
    <dbReference type="InParanoid" id="D5G9B7"/>
    <dbReference type="OMA" id="ANYDTAN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004471">
        <property type="term" value="F:malate dehydrogenase (decarboxylating) (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006108">
        <property type="term" value="P:malate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05312">
        <property type="entry name" value="NAD_bind_1_malic_enz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000182">
        <property type="entry name" value="NAD-dependent malic enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10380">
        <property type="entry name" value="Malic enzyme, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012301">
        <property type="entry name" value="Malic_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037062">
        <property type="entry name" value="Malic_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012302">
        <property type="entry name" value="Malic_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001891">
        <property type="entry name" value="Malic_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010052">
        <property type="entry name" value="PRK13529.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23406">
        <property type="entry name" value="MALIC ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23406:SF32">
        <property type="entry name" value="NADP-DEPENDENT MALIC ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00390">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03949">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000106">
        <property type="entry name" value="ME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00072">
        <property type="entry name" value="MALOXRDTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01274">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00919">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Malic enzyme N-terminal" evidence="10">
        <location>
            <begin position="95"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="Malic enzyme NAD-binding" evidence="9">
        <location>
            <begin position="283"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="570"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="573"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="118"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="189"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>(S)-malate</name>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="425"/>
        </location>
        <ligand>
            <name>(S)-malate</name>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="469"/>
        </location>
        <ligand>
            <name>(S)-malate</name>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000106-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000106-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000106-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ81110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="591" mass="65297" checksum="377672D539FA2814" modified="2010-06-15" version="1">MAPSSTMTPFLRSGTPSQIGDHHVSSAVHSAAHSRSHSPTDFDTSYNEDLRAQLKVRGLLPPHVESYELQKARCLEQIAQKSNPIDKYMYLSNLRNTNVNLFYRLLMDNMKEITPLVYTPVVGEACVKWSHIYTQPEGLYLSYADRGNLRGILDNWTADKVDIAVVTDGSRILGLGDLGVNGMGIPVGKLALYVGCAGIQPDRTLPITIDVGTENAANLKDPLYLGAKMHRVSEAEQLEFLDEMMIALNDKWPGLVVQFEDFKNPFPSLERYRNTYTCFNDDIQGTGAVVLGGTINALKNTDIPFKDQKLVFFGAGSAAVGVAKQICELFIQSGIPESDARKMFWLVDSKGMVTNDRGDTLQEHKVYFSRDDNNGQQFKSLEEVVDYVKPTALMGLSTIGGAFTESIIRKMASYNEKPIIFPLSNPSSKSECTYEQAMQWTNNKVIFASGSPFPEFEADGRVHTPGQGNNMYVFPGIGLGTVLCEAVHVTQEMVYASAVGLADSLTDEEREKGWLYPNLDRIRSISVIVARSVIRAAQKENLDKNKTLQWLSDVELDNYIRSKMYNPAGDHSGASSPTVEVNGNAAKLSNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9V0</accession>
    <name>D5G9V0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 8">Lysine--tRNA ligase</fullName>
            <ecNumber evidence="1 8">6.1.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6 8">Lysyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00005082001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>tRNA(Lys) + L-lysine + ATP = L-lysyl-tRNA(Lys) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:20792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9696"/>
            <dbReference type="Rhea" id="RHEA-COMP:9697"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78529"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.6"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="6.1.1.6" evidence="1 8"/>
    <dbReference type="EMBL" id="FN430064">
        <property type="protein sequence ID" value="CAZ81293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837102.1">
        <property type="nucleotide sequence ID" value="XM_002837056.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9V0"/>
    <dbReference type="STRING" id="656061.D5G9V0"/>
    <dbReference type="GeneID" id="9188536"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005082001"/>
    <dbReference type="eggNOG" id="KOG1885">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008255_6_0_1"/>
    <dbReference type="InParanoid" id="D5G9V0"/>
    <dbReference type="OMA" id="MQERHVD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004824">
        <property type="term" value="F:lysine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070154">
        <property type="term" value="P:mitochondrial lysyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04322">
        <property type="entry name" value="LysRS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002313">
        <property type="entry name" value="Lys-tRNA-ligase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044136">
        <property type="entry name" value="Lys-tRNA-ligase_II_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018149">
        <property type="entry name" value="Lys-tRNA-synth_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00499">
        <property type="entry name" value="lysS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42918:SF5">
        <property type="entry name" value="LYSINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42918">
        <property type="entry name" value="LYSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00982">
        <property type="entry name" value="TRNASYNTHLYS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="9">
        <location>
            <begin position="150"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ81293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="500" mass="55594" checksum="DC68492067C0DD9A" modified="2010-06-15" version="1">MNVKSVVEICREYEHIVAGCGGGDESVSIDKLVTIRGRIKNYREVSSKLIFYDLVQDGEKIQVVVNCARVGGEKGEFRRISMLTRIGDVVSVTGYPGRTKAGELTIFATAHLQILAPSLHVPPQTILDLETRLRNRHVDLMVRPVAGQILRLRSHIIQHIRDFFISRDFVEVQTPILSDTFGGAVAKPFLTEATSVCRGKQLALRIAPELWLKRLVIGGLDRVFEIGTQFRNEGVDNTHNPEFTTCEFYQAYAGLEDIINTTEELLSGMARKVSELKARKLKDIPEIETDFTAPFQRLEFIPALEASMGHKLPVLHGNTEAVTLELLSLFKTLSLPTPPMTTIPRLLQKLSSEYLEPQCMKPTFITNHPETLSPLSKTSLLHGQRVSSRVELFVDGKELVNAYEEENSPTEQRRKFVMQAGWKDEDVIERGEDGEGVVDEAYCQALEWGLPPTGGWGIGIDRLVMVFAGAERIADVLAFGGLRGAVNLGDGVRGTKEEGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>D5GA18</accession>
    <name>D5GA18_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00003499001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430070">
        <property type="protein sequence ID" value="CAZ81361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837170.1">
        <property type="nucleotide sequence ID" value="XM_002837124.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GA18"/>
    <dbReference type="GeneID" id="9187059"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003499001"/>
    <dbReference type="eggNOG" id="ENOG502SWER">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077712_0_0_1"/>
    <dbReference type="InParanoid" id="D5GA18"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072613" evidence="2">
        <location>
            <begin position="19"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="230"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="230"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ81361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="286" mass="30202" checksum="CE12DE150E9CD8B6" modified="2010-06-15" version="1">MIPTVFLTLLGTATLATATISYNSTLTHPGDDEPWFDGSIPATSISESCGSAYAQDLPCDEWLLNAFNHESSEEEKFNNSTLKALCTDTCILGLQKWRDDVRKSCTDKDVGRKASIGQQTIASLIDTKIMFVQYLYWPTCMKDLKKGTLCYADGSNGMYTASSDDTENTPEVIDAFCKNNCQTQASVFTWVVGETVFNDTADINIADARKICKGLDTSGYPFLKDSSATGGSSSSSSSSGAVGAANSTGGNGTEADTKNDGVRVGGSVGLLTTLVLGAVMVGLGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAL3</accession>
    <name>D5GAL3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00003669001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430079">
        <property type="protein sequence ID" value="CAZ81556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837365.1">
        <property type="nucleotide sequence ID" value="XM_002837319.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAL3"/>
    <dbReference type="GeneID" id="9187496"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003669001"/>
    <dbReference type="HOGENOM" id="CLU_3015904_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAL3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ81556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="56" mass="6155" checksum="29C84EE1B099D4E7" modified="2010-06-15" version="1">MAYGPSNETKKQYLSLNLHFPQPILPSQAPDSSFPPFPSCPPVHFRNGAFPAVSLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAV2</accession>
    <name>D5GAV2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NEDD8-activating enzyme E1 regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00005321001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Regulatory subunit of the dimeric UBA3-ULA1 E1 enzyme.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 4">Protein modification; protein neddylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the ubiquitin-activating E1 family. ULA1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430086">
        <property type="protein sequence ID" value="CAZ81645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837454.1">
        <property type="nucleotide sequence ID" value="XM_002837408.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAV2"/>
    <dbReference type="STRING" id="656061.D5GAV2"/>
    <dbReference type="GeneID" id="9187813"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005321001"/>
    <dbReference type="eggNOG" id="KOG2016">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019618_2_1_1"/>
    <dbReference type="InParanoid" id="D5GAV2"/>
    <dbReference type="OMA" id="KLITHQY"/>
    <dbReference type="UniPathway" id="UPA00885"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019781">
        <property type="term" value="F:NEDD8 activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045116">
        <property type="term" value="P:protein neddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030667">
        <property type="entry name" value="APP-BP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF29">
        <property type="entry name" value="NEDD8-ACTIVATING ENZYME E1 REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039099">
        <property type="entry name" value="APP-BP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="5">
        <location>
            <begin position="17"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR039099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ81645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="551" mass="61384" checksum="FE044ACC5D4B8CD6" modified="2010-06-15" version="1">MASSPPSGSPSSKEKKYDRQLRLWGANGQNRLERAHIALFGATATGCEVLKNLILPSIGRFTVIDDKLVEQADLGVNFFLDQDSLGKPRAERAAALLGELNPDVAGGFRIDNLESILSNTPGLLDPRTTEFTHLLLMSPIPTPLLLQLPAEIPIFFVHSLGFITSLRIYAPTHTIIEIHPDSLVDLRLFNPWPELSEFALEKTSTLDVPESEGGMGDHEHGHIPYVLLLLKYLEDWKQSHQGHLPGSYSEKTLFKSMIMDRMRTGVPGGIEENYVEAVNAVMGDLRTAELSSGTKEVLNDPECQSITSQTDEFWIIARAVKDFAGSNNALLPLSGAMPDMKAESRGYVALQNLYRAKAYSDAAAVERIVRDYMNRVQHPKRNQIDRDAIQLFCRHSNFLRRLNYRSIADEFSNSNEQHKGVVLGALGDWDVESSLIHHYIALRAYQEFYTGHGRAPGDTSHESCEEDYQEIRRIICEYLKGIGYPESLPERCEKVAKEIVRAGGGELHVTASLAGGIVAQEVIKIITRQYVPINNTVIFDGIGSRTQTFEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5GB15</accession>
    <name>D5GB15_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00005399001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430086">
        <property type="protein sequence ID" value="CAZ81708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837517.1">
        <property type="nucleotide sequence ID" value="XM_002837471.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GB15"/>
    <dbReference type="GeneID" id="9187949"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005399001"/>
    <dbReference type="HOGENOM" id="CLU_3335905_0_0_1"/>
    <dbReference type="InParanoid" id="D5GB15"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ81708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="38" mass="4116" checksum="62FE858040D00472" modified="2010-06-15" version="1">MLPYHLASARCYVLTDRICFFFSPSLLLKVHGGCGAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>D5GB93</accession>
    <name>D5GB93_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00000526001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430089">
        <property type="protein sequence ID" value="CAZ81786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837595.1">
        <property type="nucleotide sequence ID" value="XM_002837549.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GB93"/>
    <dbReference type="STRING" id="656061.D5GB93"/>
    <dbReference type="GeneID" id="9181497"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000526001"/>
    <dbReference type="HOGENOM" id="CLU_883366_0_0_1"/>
    <dbReference type="InParanoid" id="D5GB93"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ81786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="315" mass="34231" checksum="93CAD8687298F350" modified="2010-06-15" version="1">MSHPLPTKRPRIISFLESETIALEGTDLAKVYVHRELLSEHCGAGGDCWWSCFHSDTIKRLVEYLYQGDYTGPLPVSVPPVTPASTVFGEEATPLATDSLVYSGSLDYEGVLLGHAELFILAKTRKIGTLAKLCLARLFESMSRAEAEQTDSTFSENMVELLRYSYSHCYMNDSCAWGELQEIVSNAWVERIEWILEMPGSSLLSGEGKLVKDLMIGTVKRLIDADSRCGAQEGSEESVVEEPVAAPQQGYNGPNLFGNPGGKNSGVGLFANRGDIGENRPGNASHIFGASSNTTTPIAWGGSTLFAPVSRTDRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBA2</accession>
    <name>D5GBA2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00000536001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430089">
        <property type="protein sequence ID" value="CAZ81795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837604.1">
        <property type="nucleotide sequence ID" value="XM_002837558.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBA2"/>
    <dbReference type="GeneID" id="9181506"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000536001"/>
    <dbReference type="eggNOG" id="ENOG502S720">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_319627_0_0_1"/>
    <dbReference type="InParanoid" id="D5GBA2"/>
    <dbReference type="OMA" id="WKCCGRE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017956">
        <property type="entry name" value="AT_hook_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35711:SF1">
        <property type="entry name" value="ECTODERMAL, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35711">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00384">
        <property type="entry name" value="AT_hook"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="251"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="307"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="441"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="272"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="492"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="504"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="526"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="560"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="579"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="613"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="645"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="664"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="685"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="739"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="762"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="797"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="846"/>
            <end position="859"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="909" mass="96179" checksum="2423BC343838A80B" modified="2010-06-15" version="1">MANPSSGDAAGSTGASPTGPSPNSVKPSPKSSSSNAPHSATPTSAQAQFHRFRLSEFSRVESSPTSASPGGHCSRFRVTDFVPWASQADPPPPPPPPPPGVAVNGGRSASDGSPSSATTPRPGSFSVMHMDVDNSGPQQTSSSVTAAAGSTRGVLQKITVPSAQAQGLKQALPIHPRPLAPSPITTNGMVPYNPATVPQEAVWELSRVLNILRNTAPEMVVKIISDALISWPSVLEGSLLRRIGEIFPPLPAPSPGVPGPSNSAASPQEPPKKKKRGRPPGKKTKLNDGSSATVPIAANLASMPPPPLPYIAPGPQAQLAPVAPPQPPPTSAKRNQADAGKLLQCTRCLKYFNPTWRATPASDPSSEATCAVKHPFKDFPSGLEVVAPARRKNNYKTTWKWKCCGREVQSTSNELLMWPRKEDDRTGGWCFVGKHTTEAGVREKLGLKPGKGKKIHGDGDKKHKGGQGDSGDHSQANGEQEDPKEEGWTMLPGGGRSIAGPAGGMQQYSGHPQQQQVQQPPPPSPQGQQPTYPQQQQQHCHQQQQQQQQQVEDSPDSPPKRKRGRPKGSKTRSKAMPISTSAASSSTATVTAKDPTASTPTQQHPHRSHNQQRAHPQDQSSFWTQYTEPTSTSNTSPRHQPPTTLPAASPSTTTTSPATPQAPHHPHHARHPHPSHHPLEDPAISTADLTLLASATAAMTGTSNNPSSSNQPSTSDANTTTTDIPEKRKRGRPKGSKNGGKTLDAGGTATTGGNRGLRMKGQQQQQQPQQPQQQQQQPQQPQQQKKVERAVIPTVPEEIEVVDEDDDEEEMEDEEEEEEEEDEDEEDEDEEEEEEDAVGEDDVDVDANNTTSNAATSTAVPTTGSNTAEVGEEVEFGVSVGVGVGVGMGMSGMDGYAGLGGGDGGGWFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBR1</accession>
    <name>D5GBR1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Man(5)GlcNAc(2)-PP-dolichol translocation protein RFT1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">GSTUM_00005530001</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Intramembrane glycolipid transporter that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. RFT1 could mediate the translocation of the cytosolically oriented intermediate DolPP-GlcNAc2Man5, produced by ALG11, into the ER lumen where dolichol-linked oligosaccharides assembly continues. However, the intramembrane lipid transporter activity could not be confirmed in vitro.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Ornithine decarboxylase (ODC) antizyme protein that negatively regulates ODC activity and intracellular polyamine biosynthesis in response to increased intracellular polyamine levels. Binds to ODC monomers, inhibiting the assembly of the functional ODC homodimer, and targets the monomers for ubiquitin-independent proteolytic destruction by the 26S proteasome.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with ODC and thereby sterically blocks ODC homodimerization.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ODC antizyme family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the RFT1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ81911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837720.1">
        <property type="nucleotide sequence ID" value="XM_002837674.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBR1"/>
    <dbReference type="FunCoup" id="D5GBR1">
        <property type="interactions" value="678"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GBR1"/>
    <dbReference type="GeneID" id="9182948"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005530001"/>
    <dbReference type="eggNOG" id="KOG2864">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_346193_0_0_1"/>
    <dbReference type="InParanoid" id="D5GBR1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008073">
        <property type="term" value="F:ornithine decarboxylase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034203">
        <property type="term" value="P:glycolipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0075523">
        <property type="term" value="P:viral translational frameshifting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002993">
        <property type="entry name" value="ODC_AZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038581">
        <property type="entry name" value="ODC_AZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007594">
        <property type="entry name" value="RFT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13117">
        <property type="entry name" value="ENDOPLASMIC RETICULUM MULTISPAN TRANSMEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13117:SF5">
        <property type="entry name" value="PROTEIN RFT1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02100">
        <property type="entry name" value="ODC_AZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04506">
        <property type="entry name" value="Rft-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0688">Ribosomal frameshifting</keyword>
    <keyword evidence="7 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="58"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="166"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="232"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="375"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="418"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="449"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="482"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="510"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="573"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="594"/>
            <end position="604"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAZ81911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="816" mass="88100" checksum="5E519496777F3001" modified="2010-06-15" version="1">MPSKPPKHPPPRDERENRRSLLSASAEGVKFLILLQVTSRLLTFLMNQLLLQYLSPSLLGISVQLELFMISILYFSRESLRTALQRQPSSSTTAVSAAPTPTELPKAGSKLIEGTPAAHHQTVINLSLLTLPLGLLFAATLSLFYTHSFASSETASQPFFYESVCLYALATIVELAAEPYFALAQLGLRYKARATAESAAALIRCILTCGVTVAVAKGVVGEELRGRGVGPLGFAVGQVGYAVALLGVYMWCFLGEGGTEGWGVGLKGIEPRSSYHLTYFHKPLTSLAASMWLQSALKHLLTQGDSLIITYFTTNHVQGIYALSSNYGSLIARMLFQPIEESSRGLFAGLLSSTATPSREDITSAKATLQTLLRLYLLLSTFFISLAAPFAPFALSLIASRQWTAASDAGETLSAFCYYIPLLAINGVTEAFVQSVANERELAGQSAWMFAFSLGFAAVGWVFLKVLGWGARGLVAANGVNMLVRILWSAGFVRGYFARFEKEGEGFKEALLPGWVFLSTALGAGVLARGIVSEVIIPVNIWALLARFSLAVTPPPSLVQPVFKGSITLRPARTGRSGVPEVSSGYPTSPPYSNGGRRNGAAAGHGKGASRIIAKECERLLCRDMRRIFLGGGNNPKSVPVPMISVNSGGNNNNYSNGVDAKEMQFIEVWDYVGGASFRGFVAEKGIEKSLFLFFEHVEGTQLKHGLIALIELASECFSCDRLVICLDRKTDRIHSLIRDLGWVGFELITLSHWLQLDSTPSPQAMAPIVFPPTPPSSYSSTNARSGSFSSVSSTTSSVVDEEVTSDRWIFVGMEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBS8</accession>
    <name>D5GBS8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00005564001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ81928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837737.1">
        <property type="nucleotide sequence ID" value="XM_002837691.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBS8"/>
    <dbReference type="FunCoup" id="D5GBS8">
        <property type="interactions" value="609"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GBS8"/>
    <dbReference type="GeneID" id="9182965"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005564001"/>
    <dbReference type="eggNOG" id="KOG1230">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008722_0_0_1"/>
    <dbReference type="InParanoid" id="D5GBS8"/>
    <dbReference type="OMA" id="PSPRVGC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025183">
        <property type="entry name" value="DUF4110"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052588">
        <property type="entry name" value="Kelch_domain_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46063">
        <property type="entry name" value="KELCH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46063:SF1">
        <property type="entry name" value="KELCH DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13422">
        <property type="entry name" value="DUF4110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4110" evidence="2">
        <location>
            <begin position="540"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="508"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ81928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="642" mass="71669" checksum="405766C674FD15F8" modified="2010-06-15" version="1">MGKKDSKKKSADKKARVAEKTARKTSKKEKKSGNKRVADVDDDDVDLDSVLEEYTRQQALFLKVTETPLPVPPPARANATLAAALSGKELFLFGGETFNGAVARFFNELFVYNPAHDEWRQITSPNSPLPRSGHCVTTSQYGGGNTGSMWLFGGEFSSPKQGTFYHYNDFWRFDIGSREWARVESKSGGRGAGGGGGPPARSGHRMVAWKNYILVMGGFQDTGSTTKYLSDLWAFDTNLYTWTAISLPAHAQRPEARSSFSLLPHEQGAVLFGGYSRASSGSVAWKPVIHQDTWFLRMDQDLAKVRWERRKKPGNPPNPPRVGVTMAWHKGRGILFGGVKDEEGSDEGLESTFFNDLFAWGIDRNRFFPLVLRKPRVQKKAWNDRGGGRRDRAREAEEDLLRNLKSLEAVGKDDYDDEEEEEKKEEKGPIVIREQAMSLELPHERFNAAIAVLDDVLYIYGGTWEKGDREFTLDEMFAIDLGKLDGPEKMQIDETEIELRRKEKALRKNVKSKDPEQTLGTPGAQDPVPMEEVVEETSLDRTPHPRPFESLREFFARTSVAWLEMLVAEQETGAHKNKTVKELRKEGFAAAEDKWWACREEIRALEDEQEEAGIGEIVSLADACGLGGGGGSGGGGGAGRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCA7</accession>
    <name>D5GCA7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="14">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00000653001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the BLM10 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430108">
        <property type="protein sequence ID" value="CAZ82150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837959.1">
        <property type="nucleotide sequence ID" value="XM_002837913.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5GCA7">
        <property type="interactions" value="320"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GCA7"/>
    <dbReference type="GeneID" id="9184828"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000653001"/>
    <dbReference type="eggNOG" id="KOG1851">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000772_3_0_1"/>
    <dbReference type="InParanoid" id="D5GCA7"/>
    <dbReference type="OMA" id="ECTQLVP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016504">
        <property type="term" value="F:peptidase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032430">
        <property type="entry name" value="Blm10_mid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032372">
        <property type="entry name" value="Blm10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055455">
        <property type="entry name" value="HEAT_PSME4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035309">
        <property type="entry name" value="PSME4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021843">
        <property type="entry name" value="PSME4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32170">
        <property type="entry name" value="PROTEASOME ACTIVATOR COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32170:SF3">
        <property type="entry name" value="PROTEASOME ACTIVATOR COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16547">
        <property type="entry name" value="BLM10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23096">
        <property type="entry name" value="HEAT_PSME4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16507">
        <property type="entry name" value="HEAT_PSME4_mid"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11919">
        <property type="entry name" value="PSME4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Proteasome activator Blm10 N-terminal" evidence="12">
        <location>
            <begin position="21"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="Proteasome activator Blm10 middle HEAT repeats region" evidence="11">
        <location>
            <begin position="375"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="domain" description="Proteasome activator Blm10 middle HEAT repeats region" evidence="11">
        <location>
            <begin position="569"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="domain" description="Proteasome activator complex subunit 4-like HEAT repeat-like" evidence="13">
        <location>
            <begin position="1251"/>
            <end position="1458"/>
        </location>
    </feature>
    <feature type="domain" description="Proteasome activator complex subunit 4 C-terminal" evidence="10">
        <location>
            <begin position="1753"/>
            <end position="1841"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="17"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="797"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="798"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="828"/>
            <end position="838"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF11919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF16547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF23096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ82150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1841" mass="209012" checksum="7FAD0D51B25CCC5A" modified="2010-06-15" version="1">MAKVSDFLAAHLGANGDGQAASGATSPGAEWRDDDDGDVAARYRPRTYPYYRYLPYDTEDESERQKNLDEIIKHLYISIEAGDFAPGAVHWSRELRSWLSLKFDPPRKTRVKLVKLYYELAMAPGLDPTVAERFASMFMVLTKRKHYLRPGKDLTLDWRPLFKELKVFVLPQESGFIHSTTVKKNVRTLTKMCSFAQPFFNPEEITEMFEEFLPYFSTSFAEGAFVVVGLLNLLLPTVPPPPDKPKLQPSYYLPTLFHLWSLVNRSRVFDVNFLDLLSRLARDLLPAKGIPFGEYGIYSKEQSALIFTAILRLLEIPVGQATSPYSSVVDTNIGLAMTLDRDPRKNPVAHHIARWIVMSLSPHSLNQPDSILSNLEGLIQAVETFFHPSNTGNWTKTLSQLVYYLADFFVMRWNRERSGEMEVPEDRRLTPELKRRFVMCLKEVIFMGIYAKSSTAMSFSLSSLQSLAYLEPDLILPGALQRIYPSIQGLVEVHRTTSSLRSLQVLSRIMVRTRGFRNHVTTLLGLALPGIDANDLDKTLHTLSFVQAVAYNIPFRDLTEDEEGGVFTAYHQARDAMAFICNSLCKVNPEKALKQLIPVLIRNIRTEIEENGAASTRNTGSDVLPRDRGLVWNVSILSMCVVHIGDAVLNHKEELLEIAGFMQKRCKGMPTVHISNFIHHLLLNLTVVYTVDYGLVEPDKRCDINAWGKVYDPKEITIRWHIPSREEVEFAVELFRSQAENALEALASLTSGSSDIKSDGTGKEWSDEVSRNLVLLRLIISGISVLFDPQRIPGGTLVEKEKRDDDLEMKDADGDTGDAPGDVSGTDGSDEPMGEAQDDDVKPTYRYPAGYFFDDPGDPLYVTVHDLRDKVGGILHSVHTFLTSSQEDDVPCFNALYTAYRSWFMDVGIERSACVLDRVTRLFAADIHPFKVSGMRKNYPRPLLLRRANVYHLQRLRHNAGPRPQSVMDKTLLLDLAESCVSLYTEIRRHAQSAGESATKVIIGARPLVIPPLLTAFEDSIKNNDFRRMKGAMYSLLFGSLAKTAGRDWRYTPALIKAFISASTADKPSVQKLATSATYQVMDYGRPLERMVILNDETVRRIAPAEDLLAVITKKRDQIARKRKRIEAKKHDLALELVELAKKAHWKTASRAATIVVNLGLRFQTIAPTSMLELTTKGTVDAHPGLRGLYSGALVAYFCLIELRATCDHNYRNFLIDRLSLPNKVNVPTRSDDPKWTSKFLDAFTKPEAEYYVDHDYPGWFVWGKDFPAYEVNPEEGLQFDEVETNARKTIANLLDREWFKSFFGYLKQEPRDGSPDRFRMASSMLLQFAFELVRDGLALVTYDQLKEETEAVFEDGSDKHQHRATAEILGALVNAYRDSTVKNRDMMWAHVFPIVKRIFEEGLTPENSSYWSSFLHLVLQGKDLRRSWPILEWLTNYRLDMNSNAAFKESSKIQLLQQSIQEAGWHFRLEKPILEDFLKHIDHPYKGKGVREAMGQTLGTIYRSRHHESYKNVQYLMDTQARASSVGLTPYGPTEEFSETVHSVFKRLEQWRHERTPGQQSPSSYTAGSKTVLLWLDTTLSSHECTQLIPFFPDVFMGEMLHMMDIKEDPELMSLAYHVFRHLPNIPHPFKQDHKFISALIRIGTTSPLWHQRLRVLINMQVIYFRRLFLITAEEKERIFTCVSTMLEDPQLEVRIGAATTLSGMVRCSPVRLRQGIVQELKNKFTRMLVRNPLPKRSPAGTPTPDYTRIVITRHAAVLGLGALVQAFPYASPPPLWLPEVLATLAVKAAADPGMVGKSVKSALGEFKKTRQDTWHVDVKAFTSDQLEDLEGVLWKSYFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCF5</accession>
    <name>D5GCF5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00005866001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of selected genes principally by acetylation of nucleosomal histone H4 and H2A. The NuA4 complex is also involved in DNA repair.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EAF7 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430109">
        <property type="protein sequence ID" value="CAZ82198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838007.1">
        <property type="nucleotide sequence ID" value="XM_002837961.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCF5"/>
    <dbReference type="STRING" id="656061.D5GCF5"/>
    <dbReference type="GeneID" id="9186380"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005866001"/>
    <dbReference type="eggNOG" id="KOG4051">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_760809_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCF5"/>
    <dbReference type="OMA" id="TRIPNIW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012423">
        <property type="entry name" value="Eaf7/MRGBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13581">
        <property type="entry name" value="MRG-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13581:SF5">
        <property type="entry name" value="MRG_MORF4L-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07904">
        <property type="entry name" value="Eaf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="145"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="159"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="196"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="211"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="241"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="273"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="301"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="322"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ82198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="333" mass="36036" checksum="A0C0B2632A9C82EC" modified="2010-06-15" version="1">MPPKKKNKGRHAAADDDSPPVPEPIIFDGWTDEQETTLFKAIAEYYIGVHKHFRIIGIAELMKNHGVSDTHTNIEGIWRKLQSQYYLEGLDEREDIEDDLDTPDPSSEFTLPADEYGALMFSRRSDPNSIDSPLTVVPQTFWATGTKGKKSGRAARSESVVSGVGTGSASVNPDDTEDESARSASPAPTKTRKNAAKAGTPSTSTTTTTASRRKPPIRKKGLVTKTKPSHKRRPPAKPASSEEESDVSDDELSDLPDDVDGDEDAGTIAGTEAGEEEEPEVVAESEMGESTTAPGTPPPVPKKRGRKPGSKKAVPPPKKVARKGDVRRSTRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCT2</accession>
    <name>D5GCT2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">beta-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00006007001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.</text>
            <dbReference type="EC" id="3.2.1.21"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 3 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.21" evidence="3"/>
    <dbReference type="EMBL" id="FN430120">
        <property type="protein sequence ID" value="CAZ82325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838134.1">
        <property type="nucleotide sequence ID" value="XM_002838088.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCT2"/>
    <dbReference type="STRING" id="656061.D5GCT2"/>
    <dbReference type="GeneID" id="9187944"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006007001"/>
    <dbReference type="eggNOG" id="ENOG502QQ55">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_633387_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCT2"/>
    <dbReference type="OMA" id="VWNTENW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009251">
        <property type="term" value="P:glucan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000495">
        <property type="entry name" value="Periplasmic beta-glucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050288">
        <property type="entry name" value="Cellulose_deg_GH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026891">
        <property type="entry name" value="Fn3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42715">
        <property type="entry name" value="BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42715:SF3">
        <property type="entry name" value="BETA-GLUCOSIDASE B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14310">
        <property type="entry name" value="Fn3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01217">
        <property type="entry name" value="Fn3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibronectin type III-like" evidence="6">
        <location>
            <begin position="352"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ82325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="433" mass="49058" checksum="CC26D4AE3A7B8EAD" modified="2010-06-15" version="1">MRIPEALRVFIIRYYPGRNIAIEEYGLASTGGQAGSSDKPFNDHGHYEPGDKHAVKMLAQEASNDVEMGGGTLKLFRATPDLLQDRTLEQNVLDTATSRALRSKFTRRFSENPSSVAPQEKWKTVKPARRIGRERRKSRTLLFLTYVPWVYKLWSLRCLSISIPRLTYAQGCERWSNDKSSSPEAIKAAKEAEVAVVVAGTWSRDHQERCGQHGSSLLQRQAHNRTLDRHLRCRADKAILPKRGGRQRPHECALLEIQPLRQALHLLPSRGTWAPTSPPYCDYLKGSRFNPLGQVARERNDDLRTPVRALVAATAVTLRLRQELHHFPVLGCYYRQGQQLNIRNNSTRYGTEVVQLYVTDKIASVVTPNKELKGFLKVFLHAGEGKIVRIPVDVSELGVWNTENWYVVEKGEFVIVVGDSAEGIKSRSPFWVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCU7</accession>
    <name>D5GCU7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00000754001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430123">
        <property type="protein sequence ID" value="CAZ82340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838149.1">
        <property type="nucleotide sequence ID" value="XM_002838103.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCU7"/>
    <dbReference type="STRING" id="656061.D5GCU7"/>
    <dbReference type="GeneID" id="9182445"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000754001"/>
    <dbReference type="eggNOG" id="ENOG502SMWI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_323181_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCU7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="238"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="366"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="488"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="595"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="733"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="853"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="170"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="183"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="257"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="491"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="520"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="636"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="855"/>
            <end position="864"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="895" mass="95809" checksum="E9CBC88488D98887" modified="2010-06-15" version="1">MRINSLLNEGPSSDPHTVNLGNTSTYKAPQSIHTPTDPGFLLHHVSHNTSGAEPAASQLPSHYSPLQQPTNLSRGTISSRSSYSGPSLHSSTAATSPLEASMPPLSAAANDRSGHSARIHETTETASRTGSSFPGRTLPPLTTMVPSGPPGSIACDGPLSPHSTGHYPHQNGTEMSYFSSSRGFYQGQSPQQSQQYPQPLFPQQSHEHVRSNALMNFAVVAMETATPVGRKNLEMEAAAEAGRRQSGGSFAEDSIRRNSAPTTIASREQANRAPIPGAGREGGVASDNKPLFLDLDPALRPPSAGSLASTGRTIEEDDQAVHNSFQANTGACFTGHNASMSHESAPLFRPTPEPHTPTLADANSPDIGLSAAPGTGPLNIPSPSPQSFLNEAPKCSYCNVCTTGSPLRKVVSHIFGRNKLSTRQIPKNVWVYYCRKHYQRSRYRNPRGFARQQVLLVKRQCERLQLWGGVKDWVIKVRRREELRMNREVGENGEDADEAEDDEEGIADEDPEPNDGGPTSGENSRRNSTTVPRRRSSAGGGSNWIIRYTGMEKTIDDIYLLLGKIELEVQENGGKFPDVELLPNVDLSMAAPLVEAEGGGDEGGNGNPGDNDKQEDAEDGGVSGRKAGKKRRRSDGGSGSSGGDDGGGAKGSKKAKNIPPKKGDRKGKKGSSHAEIIPDQVSTDTGGRLEKAEVSGPFGVPLSSGDVGVSNGHWRTQSDFCRSSITLAEYHGPTHPEQVVTPPPSISSTGRSTPEFDDCQPAHTGKGRRARGSVTQDTCEAVYNLSPRRSARVLPVAVTTPKQPNFTHRNSGIGSPSPSNFTPRELAAIAGEPLHRFDFRRPEVFCFGEMFSESSNRKGPEKSKVSLGFDSGPRRPRVGKGADEVFECIRVATNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCZ4</accession>
    <name>D5GCZ4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">thymidylate synthase</fullName>
            <ecNumber evidence="1">2.1.1.45</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00006019001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.1.1.45" evidence="1"/>
    <dbReference type="EMBL" id="FN430131">
        <property type="protein sequence ID" value="CAZ82387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838196.1">
        <property type="nucleotide sequence ID" value="XM_002838150.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCZ4"/>
    <dbReference type="STRING" id="656061.D5GCZ4"/>
    <dbReference type="GeneID" id="9183850"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006019001"/>
    <dbReference type="eggNOG" id="KOG0673">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021669_0_2_1"/>
    <dbReference type="InParanoid" id="D5GCZ4"/>
    <dbReference type="OMA" id="QRENFTH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004799">
        <property type="term" value="F:thymidylate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006231">
        <property type="term" value="P:dTMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.572.10">
        <property type="entry name" value="Thymidylate synthase/dCMP hydroxymethylase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045097">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023451">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036926">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000398">
        <property type="entry name" value="Thymidylate_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03284">
        <property type="entry name" value="thym_sym"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11548:SF2">
        <property type="entry name" value="THYMIDYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11548">
        <property type="entry name" value="THYMIDYLATE SYNTHASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00303">
        <property type="entry name" value="Thymidylat_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00108">
        <property type="entry name" value="THYMDSNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55831">
        <property type="entry name" value="Thymidylate synthase/dCMP hydroxymethylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thymidylate synthase/dCMP hydroxymethylase" evidence="5">
        <location>
            <begin position="17"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="31"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ82387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="293" mass="32863" checksum="6672B5F8971B8C76" modified="2010-06-15" version="1">MSTSSTQRENFTHPEYHYLDLVRNMLTNGEQRPDKRRAGTTSLFATPTAPPQPEKPHTPVLSTKHVFQKVILYELFWFISGSTNNVPVSAVGAKILEGDGSRAYLDCIGLTERAQRDLGPVYGFQLRHIGAKYVDCHMGYPVQCKHHLRPHHHVRMESDRSGEDGVAAVSHVCQFYVLYKDGKGEGALCCVLYQRSCDMGLGVPFNIASSRLLTIMIAHVCGLEPGEFRDTMGDENGYADREDTLKVQLERAPRELPTAKINRQVDDIDDFKSEDFKRVGHDPHPGIKMNPAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDE8</accession>
    <name>D5GDE8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00006178001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430142">
        <property type="protein sequence ID" value="CAZ82541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838350.1">
        <property type="nucleotide sequence ID" value="XM_002838304.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDE8"/>
    <dbReference type="STRING" id="656061.D5GDE8"/>
    <dbReference type="GeneID" id="9184004"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006178001"/>
    <dbReference type="eggNOG" id="KOG1284">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_522950_0_0_1"/>
    <dbReference type="InParanoid" id="D5GDE8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10990">
        <property type="entry name" value="GTP cyclohydrolase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022163">
        <property type="entry name" value="GTP_CH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036144">
        <property type="entry name" value="RibA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47259">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47259:SF2">
        <property type="entry name" value="URACIL-REGULATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12471">
        <property type="entry name" value="GTP_CH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142695">
        <property type="entry name" value="RibA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GTP cyclohydrolase N-terminal" evidence="2">
        <location>
            <begin position="106"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="74"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ82541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="521" mass="57160" checksum="2D87D0702CA22FE5" modified="2010-06-15" version="1">MADSLLNQILSAVTQLQQDNKILAASVDAINGRVNALADVKKLKDDAAAAVKEGEDREKPATIITTTTAAATTATGGAAAEATEPPASPTNDVIPTVARKPNVTSRIILTTYPGQSGIDPIIMNWGEKDTTKRGPVVVSRGASTVRRRNAIGAHGGSYSIYHALAVAIQDLKVDHKPDFTNTEPAATIGPFPQWSDPKKIVAMDPLGHLSPWLYNDYINKENVEIRPTIAITKAHMKLPELESSVKAGRLKVDGRICLNECGELAVTKFAVEPVWYLPGVAERFGIDEGTLRRSLFENTGGSFPELITRNDIKLFLPPIGGLTVYCFGDPAKMSDPNVKLALRVHDECNGSDVFGSDICTCRPYLIFGIEEASPWRGYQVPRVQRSQTRNRPSQRILQEVKHCRRKGYEVPSPHARCTPLARYYEDRQNAFHVQHEVRCNCRTRGSRSMRKFMLGTLPLRSPRTCVGRRGSLGRLTYICGPLGVHSGAPSFFQFFSGAHAPPNPRGFLIPGVTSYVRRILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDZ0</accession>
    <name>D5GDZ0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00001108001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430155">
        <property type="protein sequence ID" value="CAZ82733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDZ0"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001108001"/>
    <dbReference type="HOGENOM" id="CLU_3432052_0_0_1"/>
    <dbReference type="InParanoid" id="D5GDZ0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ82733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="17" mass="1983" checksum="725CC9E33E344479" modified="2010-06-15" version="1">MYTKITTKVKKTIPCEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEH4</accession>
    <name>D5GEH4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Citrate synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00006485001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Carbohydrate metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the citrate synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430175">
        <property type="protein sequence ID" value="CAZ82917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838726.1">
        <property type="nucleotide sequence ID" value="XM_002838680.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEH4"/>
    <dbReference type="STRING" id="656061.D5GEH4"/>
    <dbReference type="GeneID" id="9181548"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006485001"/>
    <dbReference type="eggNOG" id="KOG2617">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025068_0_1_1"/>
    <dbReference type="InParanoid" id="D5GEH4"/>
    <dbReference type="OMA" id="HWRQQML"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046912">
        <property type="term" value="F:acyltransferase activity, acyl groups converted into alkyl on transfer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.230.10:FF:000002">
        <property type="entry name" value="Citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.580.10">
        <property type="entry name" value="Citrate Synthase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.230.10">
        <property type="entry name" value="Cytochrome P450-Terp, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016142">
        <property type="entry name" value="Citrate_synth-like_lrg_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016143">
        <property type="entry name" value="Citrate_synth-like_sm_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002020">
        <property type="entry name" value="Citrate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019810">
        <property type="entry name" value="Citrate_synthase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036969">
        <property type="entry name" value="Citrate_synthase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42871">
        <property type="entry name" value="CITRATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42871:SF1">
        <property type="entry name" value="CITRATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00285">
        <property type="entry name" value="Citrate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00143">
        <property type="entry name" value="CITRTSNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48256">
        <property type="entry name" value="Citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00480">
        <property type="entry name" value="CITRATE_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ82917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="519" mass="57682" checksum="5F0D8F22B39DB86D" modified="2010-06-15" version="1">MAPPPPAPKPADRNTLTGEIPETKDGVDTDDTTNGIRVFDPAFLNTAAISSKITFIDGGKGFLMHRGYPIEQLAERSDFLETAYLLIYGELPSKSQSEIWKFEVMHHTEIADDLAKVIGSMRYDSHPMSANPALQGRNLYIHDKAIMNKNIIRIIGKSATVAAASMRVRMNRPFIAPRSDMGFAENMLYMMDATSDAENYKPDARLAKALEALFILHADHEMNCSTAAMTHVGSSLVDPYSAVSAAAAALYGPLHGGANEAVIRMIEKIGAVENIPAFISAVKAKKELLFGFGHRVYRNTDPRSKIIREVAETVFAACGHEPLIEVATALKDAALADPWFVEKKLYPNVDYWSGLIYRSLGFPLDFFPVLFAVPRVAGWLAHWKQAIEASNPKIWRPRQVYVGPQVRDYVPIECRLEREIRDGKEGILGLPACILLFFPSLLKVWLNWDVLMILGVIALPVEKKTGCQVVNRLESAPKSFISFPFHFFSGWSVCVRVSLRYIIANSISPPINIKYPSTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEH8</accession>
    <name>D5GEH8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="13">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00006489001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
            <orientation evidence="2">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I NDUFB3 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430175">
        <property type="protein sequence ID" value="CAZ82921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838730.1">
        <property type="nucleotide sequence ID" value="XM_002838684.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEH8"/>
    <dbReference type="STRING" id="656061.D5GEH8"/>
    <dbReference type="GeneID" id="9181552"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006489001"/>
    <dbReference type="eggNOG" id="ENOG502SBXP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_145518_2_1_1"/>
    <dbReference type="InParanoid" id="D5GEH8"/>
    <dbReference type="OMA" id="TYCAYEA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022900">
        <property type="term" value="P:electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012576">
        <property type="entry name" value="NDUFB3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15082:SF2">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 BETA SUBCOMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15082">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE B12 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08122">
        <property type="entry name" value="NDUF_B12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0249">Electron transport</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="43"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ82921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="72" mass="8338" checksum="27381D9B41F92621" modified="2010-06-15" version="1">MTPRPPFDPQILLKKPTRPDPWARAETWRYTGPFTRWNRFKGAFPGLGIATVAFATYCAYEALFLKKDEHHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEJ7</accession>
    <name>D5GEJ7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00006523001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430175">
        <property type="protein sequence ID" value="CAZ82940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838749.1">
        <property type="nucleotide sequence ID" value="XM_002838703.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEJ7"/>
    <dbReference type="STRING" id="656061.D5GEJ7"/>
    <dbReference type="GeneID" id="9184882"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006523001"/>
    <dbReference type="HOGENOM" id="CLU_1195621_0_0_1"/>
    <dbReference type="InParanoid" id="D5GEJ7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="1">
        <location>
            <begin position="74"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="232" mass="25636" checksum="89AAE444295C10FD" modified="2010-06-15" version="1">MTEYLLKHLREMTKEEGRLSSKLGWPFLDSTWAVGGGKRENLSVGVWECMDKDLELDDNATLTFSFGEDDEKTLEVTEVVEVVAGVLLQWLRNLKEGVVPQDIWLDVYKAGADKMLAEQVLDKLFTSLSPAHANVFVYLTGFIMEIISLLSSPIPSQLPSLDADSVTSPLSPIQGVLPFGRLRAARGVVVKHAILEVFAEAIIRKEGGNKTAEDKRKAVAFLEVFVSDVEKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEW7</accession>
    <name>D5GEW7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00006620001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430197">
        <property type="protein sequence ID" value="CAZ83060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEW7"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006620001"/>
    <dbReference type="HOGENOM" id="CLU_2623790_0_0_1"/>
    <dbReference type="InParanoid" id="D5GEW7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ83060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="78" mass="8515" checksum="4EABD5295583717B" modified="2010-06-15" version="1">MVSRSLVTFSPRGTEVAATFTEVFPRGQEGYNLGSGRIAGRVWAPPSRCTRSFGCCMPEKPLCVANTGKVIEYSWQAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFE0</accession>
    <name>D5GFE0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00006848001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430208">
        <property type="protein sequence ID" value="CAZ83233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839042.1">
        <property type="nucleotide sequence ID" value="XM_002838996.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFE0"/>
    <dbReference type="STRING" id="656061.D5GFE0"/>
    <dbReference type="GeneID" id="9188181"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006848001"/>
    <dbReference type="eggNOG" id="ENOG502SUHZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_918861_0_0_1"/>
    <dbReference type="InParanoid" id="D5GFE0"/>
    <dbReference type="OMA" id="SEPHAAM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018466">
        <property type="entry name" value="Kre9/Knh1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052982">
        <property type="entry name" value="SRP1/TIP1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40633:SF1">
        <property type="entry name" value="GPI ANCHORED SERINE-THREONINE RICH PROTEIN (AFU_ORTHOLOGUE AFUA_1G03630)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40633">
        <property type="entry name" value="MATRIX PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_8G05410)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10342">
        <property type="entry name" value="Kre9_KNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072698" evidence="4">
        <location>
            <begin position="24"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="241"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="Yeast cell wall synthesis Kre9/Knh1-like N-terminal" evidence="5">
        <location>
            <begin position="40"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="135"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="149"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="181"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF10342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ83233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="303" mass="30971" checksum="ED8CF231D0E6D81E" modified="2010-06-15" version="1">MMRYLSFLPVVLCALTGAIGAFAQGTSGQNAITYPLGGSLDSTVPLTITWTPTTQGTITISLLKGPPSNLIDLGPIAASISNGGTFTWTIPNSLQDSGSMPKGDKYGFKIIDDATKTFEYSPPFDLSIPDSTFGGATASGTTNPTASLTTSISSAPSQPSSSTATPTPTSASTATEESTSDLPSTTTQPLVPTSTDQPNGTPTSLEISLQSTLAAATTTSVPKPPQSKAADILAPNTGPNVIMIIGVAAGAFGGLALLIVGIVVFIKCRKRRSRARARAPLVSVQPPVGGMRGYGVDGMYNRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFH7</accession>
    <name>D5GFH7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00006905001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430208">
        <property type="protein sequence ID" value="CAZ83270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839079.1">
        <property type="nucleotide sequence ID" value="XM_002839033.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFH7"/>
    <dbReference type="GeneID" id="9188218"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006905001"/>
    <dbReference type="HOGENOM" id="CLU_2795785_0_0_1"/>
    <dbReference type="InParanoid" id="D5GFH7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ83270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="68" mass="7117" checksum="D894BB78436C5E68" modified="2010-06-15" version="1">MRGAKARGKATEDDAIYITVASRAALDVMGVGAVAYKDSKVILRKGGSGVLVAVYKEKDSLGESPDEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFI3</accession>
    <name>D5GFI3_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">alpha-L-rhamnosidase</fullName>
            <ecNumber evidence="2">3.2.1.40</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00006913001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal non-reducing alpha-L-rhamnose residues in alpha-L-rhamnosides.</text>
            <dbReference type="EC" id="3.2.1.40"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.2.1.40" evidence="2"/>
    <dbReference type="EMBL" id="FN430208">
        <property type="protein sequence ID" value="CAZ83276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839085.1">
        <property type="nucleotide sequence ID" value="XM_002839039.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFI3"/>
    <dbReference type="GeneID" id="9188224"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006913001"/>
    <dbReference type="eggNOG" id="ENOG502QXBB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002926_1_1_1"/>
    <dbReference type="InParanoid" id="D5GFI3"/>
    <dbReference type="OMA" id="LLCEECP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030596">
        <property type="term" value="F:alpha-L-rhamnosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.420.10">
        <property type="entry name" value="Maltose phosphorylase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016007">
        <property type="entry name" value="Alpha_rhamnosid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035396">
        <property type="entry name" value="Bac_rhamnosid6H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035398">
        <property type="entry name" value="Bac_rhamnosid_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013737">
        <property type="entry name" value="Bac_rhamnosid_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008902">
        <property type="entry name" value="Rhamnosid_concanavalin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33307">
        <property type="entry name" value="ALPHA-RHAMNOSIDASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33307:SF6">
        <property type="entry name" value="ALPHA-RHAMNOSIDASE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05592">
        <property type="entry name" value="Bac_rhamnosid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17389">
        <property type="entry name" value="Bac_rhamnosid6H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17390">
        <property type="entry name" value="Bac_rhamnosid_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08531">
        <property type="entry name" value="Bac_rhamnosid_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25788">
        <property type="entry name" value="Ig_Rha78A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010631">
        <property type="entry name" value="A-rhamnsds"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bacterial alpha-L-rhamnosidase N-terminal" evidence="6">
        <location>
            <begin position="145"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-L-rhamnosidase concanavalin-like" evidence="5">
        <location>
            <begin position="326"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-L-rhamnosidase six-hairpin glycosidase" evidence="7">
        <location>
            <begin position="433"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-L-rhamnosidase C-terminal" evidence="8">
        <location>
            <begin position="786"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="857"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="905"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="21"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="916"/>
            <end position="928"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ83276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="928" mass="103019" checksum="5BD3540D271DD61D" modified="2010-06-15" version="1">MSVDSITFDRQRPTKHLLSAPSKSPTLSWRTTPNKSDWQQTTYQIRLDRPDWQPTTYYVNSAASVAVTWPGADISSREIVNVKVAVRGVGEEQDVWSNEVSVEGGLFEQEDWSAVPICAVGVADLTGRVPVTLLRKEFTLRDGDKMVKRARLYVTALGVYEVEINGRKVGDEEFAPGWTSYGHRIKYQTFDVADLLRKGENAVGAYLGEGWYRGRIGFEGGRTAIYGKDIGLIFQLEITYEDDTVTKVVSDDSWKASTGAILYSEIYDGEFYDARKEKPGWSSPGYTGSSDWKAVRELPFPTAALVSPPGPPVRCTEIVAPVKVFTTPSGKTVVDFGQNLVGRLRIKLCGPSGHKVTFTHTEVLEKSGEVATRPLRAAQCTDHYIMKGSDEPETYEPKFTFHGFRYVQVDNWPPISDLLKSVEARVLHTDMQRTGWFSCSDKLINRLHRNVVWGMRGNFLEIPTDCPQRDERLGWTGDIHVFSETASFLYDCSGMLQGWLQDLAFEQMDNDHCVPPLVVPDVIDPGKFPRLPQAVWQDVAIVLPWVLYTRFGDVNILYSQLASMKSWLSSGIPRDPATGLWSRTFQFGDWLDPSAPGDAPQDGCTDPMLVANAFLIHVTDLLSRISTILGDPDGAAAYATSACTLRQAFNQEYVTPAGRLSSDSQTAYVLALRFNILPTPTQVEAASKRLAEVVQKSKFRIATGFAGTPYILPTLTTTGNRQLAYRMLHEQKCPSWLYPLQHDATTIWERWDGILPDGSINPAEMTSFNHYALGAVAEWMHASIGGIAPAAPGWKRIWVAPVPGGEVSWAEVRFLSVYGEVYVRWEVKDGRFKLKVRIPGNVSAGIVFPRGSRDEDVLVEESAGGGSEEEEEEAGKAGDKDTGAQVLEVGSGVHVFDRVWKEDTDWPPAVSSHPYMPEKEAVSEGKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFL0</accession>
    <name>D5GFL0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Class E vacuolar protein-sorting machinery protein HSE1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Class E vacuolar protein-sorting machinery protein hse1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">GSTUM_00006968001</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the ESCRT-0 complex which is the sorting receptor for ubiquitinated cargo proteins at the multivesicular body (MVB).</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the ESCRT-0 complex composed of HSE1 and VPS27.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endosome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the STAM family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430209">
        <property type="protein sequence ID" value="CAZ83303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839112.1">
        <property type="nucleotide sequence ID" value="XM_002839066.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFL0"/>
    <dbReference type="FunCoup" id="D5GFL0">
        <property type="interactions" value="331"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GFL0"/>
    <dbReference type="GeneID" id="9188251"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006968001"/>
    <dbReference type="eggNOG" id="KOG2199">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010104_1_1_1"/>
    <dbReference type="InParanoid" id="D5GFL0"/>
    <dbReference type="OMA" id="MMDAWEK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033565">
        <property type="term" value="C:ESCRT-0 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21386">
        <property type="entry name" value="GAT_Hse1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11805">
        <property type="entry name" value="SH3_GRB2_like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16978">
        <property type="entry name" value="VHS_HSE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000072">
        <property type="entry name" value="Unconventional Myosin IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004152">
        <property type="entry name" value="GAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050670">
        <property type="entry name" value="STAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002014">
        <property type="entry name" value="VHS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45929">
        <property type="entry name" value="JAK PATHWAY SIGNAL TRANSDUCTION ADAPTOR MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45929:SF3">
        <property type="entry name" value="JAK PATHWAY SIGNAL TRANSDUCTION ADAPTOR MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03127">
        <property type="entry name" value="GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00790">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01887">
        <property type="entry name" value="SPECTRNALPHA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00288">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50179">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0967">Endosome</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="VHS" evidence="15">
        <location>
            <begin position="16"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="14">
        <location>
            <begin position="218"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="184"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="375"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="191"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="513"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="568"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="587"/>
            <end position="618"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAZ83303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="648" mass="70391" checksum="883E66B59B5CEA77" modified="2010-06-15" version="1">MFRAQQNEFDDVVAKATDETLTSENWGYMMDAWEKINDSGENGPKNSVAALIKRLAHRNADVQLYTLELANAFSQNCGPKMHRELASRSFTEALLRLAGDRNTHQKVKTKILERMAEWSEMFSKEPDLGIMDQAYNKLKSQNPTIQAPSKPHKKEITDVDRQKEEEELQMALAISLKDRTAQGVAAAKATQQPAGSGQPSQAQGQAGPSQVAPHTTAATVSRVRALFDFVPSEAGELAFRKGDIIAVLESVYKDWWKGSLRGQTGIFPLNYVEKLSDPTPEEVQREAQMEAEVFAEIKNVEKLLALLSTTSVDTNVQDNEEITTLYHQTLAIRPKLIELIGKYSQKKDDFMSLNEKFIKARKDYESLLETSMAQPASHYGRPNQSPYNYGGDAYPTGGGEPGRYYTPAPQHNAASPSNAHPTFAPPQQKPTGAPYPGGRRTSNTFYPPQGDSLDNPSHAGGAPFYVVGQAPPGTQLPGRQGPSSPSMPSADFHPQRPHPSHTPSGSGAPPTDGPSQPQELATSAYDSPMESRPPGGGFNPQPSKLQSTDRPHSPKAPGQPSYTGYHYQPQSRPLSAGTNSNQPYPESSGPPSVYPSQPYPSLTPQSTTPGGTSNPSTPYNTYSSPAVGAPPQPNNNELFLPPGSRAQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>D5GG06</accession>
    <name>D5GG06_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">histidine kinase</fullName>
            <ecNumber evidence="3">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">GSTUM_00007138001</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="3"/>
    <dbReference type="EMBL" id="FN430231">
        <property type="protein sequence ID" value="CAZ83449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839258.1">
        <property type="nucleotide sequence ID" value="XM_002839212.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GG06"/>
    <dbReference type="FunCoup" id="D5GG06">
        <property type="interactions" value="144"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GG06"/>
    <dbReference type="GeneID" id="9181794"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007138001"/>
    <dbReference type="eggNOG" id="KOG0519">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003731_0_0_1"/>
    <dbReference type="InParanoid" id="D5GG06"/>
    <dbReference type="OMA" id="WGDSNRI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009927">
        <property type="term" value="F:histidine phosphotransfer kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005034">
        <property type="term" value="F:osmosensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007234">
        <property type="term" value="P:osmosensory signaling via phosphorelay pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.130:FF:000004">
        <property type="entry name" value="Ethylene receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000289">
        <property type="entry name" value="Osmosensing histidine protein kinase SLN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43047:SF72">
        <property type="entry name" value="OSMOSENSING HISTIDINE PROTEIN KINASE SLN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43047">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 14" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="391"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="18">
        <location>
            <begin position="566"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="19">
        <location>
            <begin position="891"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="331"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="424"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="720"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="525"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="430"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="741"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="14">
        <location>
            <position position="946"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CAZ83449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1066" mass="117139" checksum="D12500FD0F47B18C" modified="2010-06-15" version="1">MRIRIREQLSLLVALTALIAVGVLAVVTWVHNYRLVREVRSSRFSLTASLKSIQVTQSIALLHNTVYAISTRAILQSSLRRYQRGNNTADNWLRAIDDLKNSASASIGGFPYVLQVVVYDENLSSGMVGVPDGAVIDGMQLSATNPNFTGRLPNGTEIKVGSGLVKDGFPHTLYPFLHGSHALAPSNVFTPEDIYSRKGLLLGPMRVNESFYMLSFTVPVINNTSEATLLGFLTVVLNAGVILDIVRDTRGLGATGQTILVGPATINNKWENPTLSATSEMPNTTKNGQSDHTRTFRYLLPPGRNPELAGEVRKLKDYSMIEKVYQDGIGVNGRDGDSGDGGGSNLDTRNSEDRLVSVGYGFPASNLADWALLVEQDQEEAFKPIYKLRNILLSTVFGTFAVVIVLVCPVAHFAVKPITRLKRATEKSTRPPSYSLDSGSSGTIDSFDPEANLENSADRDRGRNRFYGMGELMRCRHPRNDSSQSGDGSDDQRRRAFRIPGKVQERKHFVDDELTDLTRTFNEMSEELVKQYVNLEERVAERTKELEEQKKVAESANQAKSLFVANITHELRTPLNGILGMCAVSMQEDDLPKIKRSLGVVYKSGELLLHLLTDLLTFSRNQVGHMAISLDEKEFRMAEISAQIKAIFETQANESKIDFSVSIAPEKRVQEMVFWGDSNRILQVLINLVSNSLKFTPENGAVHVRMRLLQEVLPVPEESTILRGSSRTGSKHDHGSKAVSRINSTNTPRNGSPAVSSPPPTSQAASPPTIEKVGDHVLGDGPVLAPPPNSKTYMFEFQVEDTGPGIPENLHQKVFEPFVQGDLRLSKKYGGTGLGLSICDQLARLMQGTIELKSLEKAGTTFTLKIPLKQAKEYTPSVGARGGDEDTGRVRVLVAEDNKVNQEVVLKMLRLEEIYDVTIAKDGQEAVDRIKEALDVGKHFQLVLMDIQMPNLDGIESTKIIRHLGYDAPIVALSAFAEEGNVKDCLDAGMNYFLAKPIKRPELKQVLKTYCPTIKEECAFSTEQHAEGSASVRTTPMTTPPMETAEKMSIGGPIDCSGGILGRGRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GG10</accession>
    <name>D5GG10_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007146001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430231">
        <property type="protein sequence ID" value="CAZ83453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839262.1">
        <property type="nucleotide sequence ID" value="XM_002839216.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GG10"/>
    <dbReference type="GeneID" id="9181798"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007146001"/>
    <dbReference type="HOGENOM" id="CLU_1741896_0_0_1"/>
    <dbReference type="InParanoid" id="D5GG10"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="150" mass="16705" checksum="5136F56B2097EFC3" modified="2010-06-15" version="1">MKEGSSDSSFTNPSPGHQKRAKTGESRSLYPAPHSSTRTMTYAMTKYHTNLGTVRVLYKYSNYSTKNAIPIEPCTSNTISLQSPSPPQSRTIPIKQKLPHGTPRWNDYATIIALLAWRTNLRGPSNQQWGGLFSAYMMRTVSHADTVADL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>D5GG30</accession>
    <name>D5GG30_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="16">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">GSTUM_00001967001</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Siroheme synthase involved in methionine biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>uroporphyrinogen III + 2 S-adenosyl-L-methionine = precorrin-2 + 2 S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32459"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57308"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:58827"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.1.1.107"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 12">Belongs to the precorrin methyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430234">
        <property type="protein sequence ID" value="CAZ83473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839282.1">
        <property type="nucleotide sequence ID" value="XM_002839236.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GG30"/>
    <dbReference type="FunCoup" id="D5GG30">
        <property type="interactions" value="381"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GG30"/>
    <dbReference type="GeneID" id="9182356"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001967001"/>
    <dbReference type="eggNOG" id="KOG1527">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011276_2_2_1"/>
    <dbReference type="InParanoid" id="D5GG30"/>
    <dbReference type="OMA" id="TPCAIVE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004851">
        <property type="term" value="F:uroporphyrin-III C-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019354">
        <property type="term" value="P:siroheme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000103">
        <property type="term" value="P:sulfate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11642">
        <property type="entry name" value="SUMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1010.10:FF:000006">
        <property type="entry name" value="Siroheme synthase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000504">
        <property type="entry name" value="Siroheme synthase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.950.10:FF:000005">
        <property type="entry name" value="Uroporphyrin-III c-methyltransferase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="entry name" value="Methyltransferase, Cobalt-precorrin-4 Transmethylase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006366">
        <property type="entry name" value="CobA/CysG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012066">
        <property type="entry name" value="Met1_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028162">
        <property type="entry name" value="Met8_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050161">
        <property type="entry name" value="Siro_Cobalamin_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028281">
        <property type="entry name" value="Sirohaem_synthase_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003043">
        <property type="entry name" value="Uropor_MeTrfase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45790">
        <property type="entry name" value="SIROHEME SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45790:SF6">
        <property type="entry name" value="UROPORPHYRINOGEN-III C-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13241">
        <property type="entry name" value="NAD_binding_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14823">
        <property type="entry name" value="Sirohm_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14824">
        <property type="entry name" value="Sirohm_synth_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036555">
        <property type="entry name" value="SUMT_yeast"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75615">
        <property type="entry name" value="Siroheme synthase middle domains-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00840">
        <property type="entry name" value="SUMT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="2 12" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Siroheme synthase central" evidence="15">
        <location>
            <begin position="138"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Siroheme biosynthesis protein Met8 C-terminal" evidence="14">
        <location>
            <begin position="219"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="13">
        <location>
            <begin position="277"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00052360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00055636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF14823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF14824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAZ83473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="525" mass="57480" checksum="25F32BCEBAD19AF5" modified="2010-06-15" version="1">MPGLTTSPGFLTSFDCSGQVHLIVGSNPLAGARCAKSLEVGAKVKLIAPDSEALHYGLRQRIETGEVQWIRREFQSEDLTTLGRVEVDLVVDLVFVTLSIECAQCTYISNHCRRLRIPVNVADAPHLCSFTLLSTYSDGPLQIGVTTSGNGCKLSNRIKREITNTLPQGMGTACTRLGKLRRRLWEEDHGEADIFDEDVDGMDQKSNFNALILPEDVDAAKTRRTRWLSQICEYWPLEKLAGLTDDDIGSILESYIDANNSGDVPQPLGSLPHRGRIQLVGSGPGHPDLLTTASVNAISSADLVLADKLVPQPVLALVPRRVVVHIARKFPGNADKAQEEFLKLGLEGLQKGLNVIRLKQGDPYIYGRGAEEFEFFESRGYTPTVLPGITSSLSAPLFACIPPTHRGVADQALICTGTGRKGTAPEIPEYVSTRTTVFLMALHRLGEMVRDLEKKGWPMELPCAVIERASCSDQRIVRSTLENVCFAVEEVGSRPPGLIVVGWACEVVKKLDGKKWVVEEKGFNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>D5GG35</accession>
    <name>D5GG35_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">chitinase</fullName>
            <ecNumber evidence="2">3.2.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00001975001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.14"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the glycosyl hydrolase 18 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.14" evidence="2"/>
    <dbReference type="EMBL" id="FN430237">
        <property type="protein sequence ID" value="CAZ83478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839287.1">
        <property type="nucleotide sequence ID" value="XM_002839241.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GG35"/>
    <dbReference type="GeneID" id="9184539"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001975001"/>
    <dbReference type="eggNOG" id="ENOG502RK0Z">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060983_1_0_1"/>
    <dbReference type="InParanoid" id="D5GG35"/>
    <dbReference type="OMA" id="AEDPRMY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008843">
        <property type="term" value="F:endochitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001579">
        <property type="entry name" value="Glyco_hydro_18_chit_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050542">
        <property type="entry name" value="Glycosyl_Hydrlase18_Chitinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45708">
        <property type="entry name" value="ENDOCHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45708:SF60">
        <property type="entry name" value="III CHITINASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G03850)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01095">
        <property type="entry name" value="GH18_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0146">Chitin degradation</keyword>
    <keyword evidence="6 8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GH18" evidence="10">
        <location>
            <begin position="13"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ83478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="293" mass="32607" checksum="CC32C28D78508D68" modified="2010-06-15" version="1">MPPPPLPPRPKTPRIILYHQTHYTSSKEYISILPLLTENTGTTHVILAAIHLNNPPGNIHLNETPPDDAIFETLWDEVLVLQDSGIKVLGMLGGAARGSFERLDGSEQSFEAYYCPLRDMVHKHKLDGLDLDVEEEMSLEGIIRLIDRLKADFGQEFLVTLAPVATGLINQRHLSGFDYQMLEVLRGGVIDWYNVQFYCGWGSMADPQCYDLIMAMGWPPEKVVAGVVTNAANGSGWVGIDVLVVVFMELVQKYPGFGGVMGWEYFNAMPGGLGRPWEWAENMANAMAMGRAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGE2</accession>
    <name>D5GGE2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00007336001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430264">
        <property type="protein sequence ID" value="CAZ83585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839394.1">
        <property type="nucleotide sequence ID" value="XM_002839348.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGE2"/>
    <dbReference type="GeneID" id="9183183"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007336001"/>
    <dbReference type="HOGENOM" id="CLU_2980783_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGE2"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ83585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="58" mass="6874" checksum="DD231090A3158B70" modified="2010-06-15" version="1">MELDKLKAATALNLLKEEYARRMNGKLCLRQKELLWVYANGRMETYKQTSYNNTMISE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGI5</accession>
    <name>D5GGI5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00002033001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430265">
        <property type="protein sequence ID" value="CAZ83628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839437.1">
        <property type="nucleotide sequence ID" value="XM_002839391.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGI5"/>
    <dbReference type="GeneID" id="9185551"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002033001"/>
    <dbReference type="HOGENOM" id="CLU_1644955_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGI5"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="161" mass="18359" checksum="34782A9610BC58B9" modified="2010-06-15" version="1">MPRLAEGSHDAFFTLLASILALIPLATSLRLQRERLMMFLPRLLFQSPKEVAIHRKAITDGFTEIKGWIKTMMVFGGGTIFFGFGSLAYKVMVYDVDAKKEMMAHTARVVHESEERLQTRFGSPLDNFEQKTQAQIENLKLETKSEIDKLGHLVELSTKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGK2</accession>
    <name>D5GGK2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Translation initiation factor IF-2, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00007533001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430275">
        <property type="protein sequence ID" value="CAZ83732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839541.1">
        <property type="nucleotide sequence ID" value="XM_002839495.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGK2"/>
    <dbReference type="FunCoup" id="D5GGK2">
        <property type="interactions" value="468"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GGK2"/>
    <dbReference type="GeneID" id="9181702"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007533001"/>
    <dbReference type="eggNOG" id="KOG1145">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006301_8_6_1"/>
    <dbReference type="InParanoid" id="D5GGK2"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01887">
        <property type="entry name" value="IF2_eIF5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03702">
        <property type="entry name" value="IF2_mtIF2_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03692">
        <property type="entry name" value="mtIF2_IVc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000008">
        <property type="entry name" value="Translation initiation factor IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10050:FF:000001">
        <property type="entry name" value="Translation initiation factor IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000019">
        <property type="entry name" value="Translation initiation factor IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10050">
        <property type="entry name" value="Translation initiation factor IF- 2, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00100_B">
        <property type="entry name" value="IF_2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053905">
        <property type="entry name" value="EF-G-like_DII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044145">
        <property type="entry name" value="IF2_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006847">
        <property type="entry name" value="IF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000178">
        <property type="entry name" value="TF_IF2_bacterial-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015760">
        <property type="entry name" value="TIF_IF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023115">
        <property type="entry name" value="TIF_IF2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036925">
        <property type="entry name" value="TIF_IF2_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381:SF20">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR IF-2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR IF-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22042">
        <property type="entry name" value="EF-G_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11987">
        <property type="entry name" value="IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04760">
        <property type="entry name" value="IF2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52156">
        <property type="entry name" value="Initiation factor IF2/eIF5b, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Tr-type G" evidence="12">
        <location>
            <begin position="541"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="32"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="402"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="827"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="98"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="196"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="241"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="407"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="438"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="831"/>
            <end position="846"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ83732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1078" mass="117097" checksum="3EF51724576BD839" modified="2010-06-15" version="1">MWKKSSSLPLSLLYAAGNPYRLYTRLLHSASESDASGSTAVAEDDEEPSYTTSDSFKSFRKQGAYSIRRPEARWSVPPSNAHGSADNGMRNGPSPRWSSGGASDSARPAPSSAPIGRHWVNQQSSYGPPSRRDNTGRAAAGGDPDHKFGSRPSWSSRPAPKPAAAEGGAHDTRPPPPSGPTLSWANSRTKPDTPERGNPGPSSGPFSSRPRWNDPPPQRPYQRTKQPSDISPIRASGGGRTIDHSGPEVSDRFRPGGSQEGPIGYTRGVGQVGPRFDTGVGKGSQSESLPVKTAHSGLGRSTATDRESPIYRTYTPNAPDDIKTFRYHSQKVSGSVECFSRTQEMNRMIEEISATYVPIKTKEQDAEQRNAQEGEAGITGFTSDNVARELRTQPLTPAELEYERRQSAKARGRSRRPGFGNGESAILGEDGGPLTGKAAKKAKKAKKREKQVEAAPAMVPLYLPEYISVANLARAVKMRLDEFIVQMQNMGFEDVSYDHVMNAEVAGLIAMEYGYEPIADISEEKDLLPRPPPEDRSVLQPRPPIVTIMGHVDHGKTTLLDWLRKSSIVATEHGGITQHIGAFSVTMPSGRIITFLDTPGHSAFLSMRERGANVTDIVILVVAADDSVKPQTIEAIKHAKAANVPIIVAINKVDKEGSDIDGVKHDLAKNGVDIEDFGGDTQVVLVSGKTGQGMGELEEATVTLSEVLDMRAETDGPCEGWVLEAGTEKRGRVATVLVNRGTLRPGDIIVAGQTWARVRSLINEHGVGVEFAAPGTPVKVDGWRDQPLAGDFVIQAVSEDKAKTAINFRMEKAERLRQAADIEAINNQRRAQREKEAAEKEIRERALANNQDPDAAVAASEKLAAESRKKYEVVNFIVKGDVAGSVEAVVEAVSPLGNEEVRARVIRSSFGAVTEFDIDHAAAAEGYVLCFNLSVESEIIQYARNKGVTIMTHNVIYRLVDEVSAKLSEKLAPLIKHSVKAEAEILQIFSINVRRRKTTPVAGSQVKNGNLYKHDKVRVLRDGKIIYDGEFESMKIFTKEATEVKKGGECGLGFKGWGDFQPGDTIQTYEVIEEARTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGW1</accession>
    <name>D5GGW1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00002062001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430284">
        <property type="protein sequence ID" value="CAZ83754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGW1"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002062001"/>
    <dbReference type="HOGENOM" id="CLU_2814289_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGW1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="67" mass="7505" checksum="A56E02E081221F11" modified="2010-06-15" version="1">MGLTLNKISYLVLTNILIILLGSLLVIFWVNSFAYDPSLFSLLVPILSPIKSYTNILENKNKAGVYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGX5</accession>
    <name>D5GGX5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00002078001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430292">
        <property type="protein sequence ID" value="CAZ83768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839577.1">
        <property type="nucleotide sequence ID" value="XM_002839531.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGX5"/>
    <dbReference type="STRING" id="656061.D5GGX5"/>
    <dbReference type="GeneID" id="9181740"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002078001"/>
    <dbReference type="eggNOG" id="ENOG502QVGD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016694_2_0_1"/>
    <dbReference type="InParanoid" id="D5GGX5"/>
    <dbReference type="OMA" id="MIRNAFG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035838">
        <property type="term" value="C:growing cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051380">
        <property type="entry name" value="pH-response_reg_palI/RIM9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009571">
        <property type="entry name" value="SUR7/Rim9-like_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28013">
        <property type="entry name" value="PROTEIN DCV1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28013:SF3">
        <property type="entry name" value="PROTEIN DCV1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06687">
        <property type="entry name" value="SUR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072083" evidence="7">
        <location>
            <begin position="27"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="118"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="150"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="204"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="461"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="256"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="278"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="296"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="368"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="382"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="409"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="508"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ83768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="519" mass="54227" checksum="BC3A4CAB385839D4" modified="2010-06-15" version="1">MRLRPATPLSILLFAAFALLLLSVLSTPVVTSIHLASYKDIAFGVFGFCDANKCSGVKIGYDMGSLSPANSDFSLPSNARNSLSNLLIVHPIAALFTLILLVLAVISHLHSPAHSPRYLLALLILSLPTFLLSLLAFLVDILIFLPHVQWGGWVVLGATILIAVAGVVMCGMRRTIVSKEARRKRIEDNAAMNGQEFYASQALARERAAAPTQPDKAPEFATFEVNKTAPGEPEGERILLNPRSRTVSPPIPGTASDGSFTRSNTLTREPSDRSVGYGPPPRGAGPLSRPMPPGQVRNQYSNGTLQIGTSGSPGPYGTPPPQRGGYDRGYGGDVYGERGPPLGGFRGPTPPGMYPPAGGLAPPPRRGPSPGQLGPRPAPGNAYGGGGPGYGMGPLQNSGVMSPPPPQGGVRGGQAPYGGMGGPLPGTMMMGGRGGPMPPPGPRQYDYPEDEIIDERREFEKTLPIAGVVGGTGRDELGGRPSGPGGPVELDTDERPSEPPHTGRLSVVNENGGSTSQDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5GH10</accession>
    <name>D5GH10_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00007648001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430297">
        <property type="protein sequence ID" value="CAZ83803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839612.1">
        <property type="nucleotide sequence ID" value="XM_002839566.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GH10"/>
    <dbReference type="STRING" id="656061.D5GH10"/>
    <dbReference type="GeneID" id="9185057"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007648001"/>
    <dbReference type="eggNOG" id="ENOG502S0C3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037393_1_0_1"/>
    <dbReference type="InParanoid" id="D5GH10"/>
    <dbReference type="OMA" id="SWKPAYL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd13298">
        <property type="entry name" value="PH1_PH_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13299">
        <property type="entry name" value="PH2_PH_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000286">
        <property type="entry name" value="PH-protein kinase domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051707">
        <property type="entry name" value="PI-Interact_SigTrans_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14336:SF15">
        <property type="entry name" value="DUAL ADAPTER FOR PHOSPHOTYROSINE AND 3-PHOSPHOTYROSINE AND 3-PHOSPHOINOSITIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14336">
        <property type="entry name" value="TANDEM PH DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="44"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="270"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="224"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="378"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="230"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ83803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="398" mass="43560" checksum="FFD2A6AC7C28B85F" modified="2010-06-15" version="1">MSTAEVLSPTSPVSINMAASPLPHLRTRANPTAVDQDGSFQMDKVIKSGWLLKRTRKTKNWKRRWFVLRGDRLSCYKDQKEYKIHRQIYLAELTAVAVLKDAKKPNVFGLFSPSKNHHFQGESAEDTTEWVQLIKGLTSIVETEDELFLNSPTQLHDPANPLSSPTAYNPNMRLGSSSPEFGSNPGAVQVRGMGRGGISTHTLEYSGAEVGSFSSVSDGARISQLSLSHPDPGIGPSSGAAGAGASAEPPHPGVTRHESGFSSDHMDDSRVVWHGYLYCLKSKGGVKQWKRYWVVLRTVHLAFYKNEEEYRAIKIIHLDSVVDAVEIDPLSKSKKHCLQVITEGKSLRFAAADEESLAKWLGALKSTLTKRALMKTAAVTSPTTANPPTSSSGPSQQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHM8</accession>
    <name>D5GHM8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00008050001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the cullin family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430320">
        <property type="protein sequence ID" value="CAZ84058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839867.1">
        <property type="nucleotide sequence ID" value="XM_002839821.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHM8"/>
    <dbReference type="FunCoup" id="D5GHM8">
        <property type="interactions" value="777"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GHM8"/>
    <dbReference type="GeneID" id="9183712"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008050001"/>
    <dbReference type="eggNOG" id="KOG2167">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004747_7_0_1"/>
    <dbReference type="InParanoid" id="D5GHM8"/>
    <dbReference type="OMA" id="NYQEQTW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031461">
        <property type="term" value="C:cullin-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000014">
        <property type="entry name" value="Cullin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000031">
        <property type="entry name" value="Ubiquitin ligase subunit CulD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1310.10">
        <property type="entry name" value="Cullin Repeats"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.130">
        <property type="entry name" value="Cullin, Chain C, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045093">
        <property type="entry name" value="Cullin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059120">
        <property type="entry name" value="Cullin-like_AB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016157">
        <property type="entry name" value="Cullin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016158">
        <property type="entry name" value="Cullin_homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036317">
        <property type="entry name" value="Cullin_homology_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001373">
        <property type="entry name" value="Cullin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019559">
        <property type="entry name" value="Cullin_neddylation_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11932">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00888">
        <property type="entry name" value="Cullin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26557">
        <property type="entry name" value="Cullin_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10557">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00182">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00884">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75632">
        <property type="entry name" value="Cullin homology domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01256">
        <property type="entry name" value="CULLIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50069">
        <property type="entry name" value="CULLIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Cullin family profile" evidence="7">
        <location>
            <begin position="505"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="90"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="20"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="61"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ84058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="873" mass="99895" checksum="B869732C48CCA9CC" modified="2010-06-15" version="1">MPEKVSDQETQSQGKKRKQNLQSPRPSQGFSQRSITDFFGDSTLGLDVPTSRPSSPKRSRKNSEQPELPKDMSTPLNEQAWRRISENRVADLNGLPPHPRDPPNPNTNIVATKPPRPAPFQPHAGAPRKLHIKNLRKPSKIDPDVYFNQTWGSLEAALAAIFGSRKISASLEELYRGTENICRADRAGELYIRLKACCATYVGDYLKDSIIACNSWKDDAVKCVVSAWEKWNAQLGMIRSVFLYLDRSYLLNNANPSLQPVEPTGLELFRHHIILAQEIETKFMDGIMALFERDRQQCSIDASLLTRAVRMVDSLDLYETNFEPRFLAMSREYYDRLGILGATSNSLAEYLDECSQQLHKEALRCERYRLDPPTKRSMGLILEEGLLKNQLLILTDQGSIEDLLQKQDHKSLATLYSLLDRIGEPSSYLRLAWEKHILTVGRSIIEDESRENEMVQRLLELKDSLDSFVRVPFKGDDTLAYALRESFGTFLNARTKDRSEMVNSKPAEMIAKYVDALLRGGAKGTSTGTPGDEDARLAHSLEQVLDLFRFIQGKDVFEAFYKRDLARRLLMDRSASRDAERSMITKLKTECGSGFTQNLESMFKDIEISREAISHFKTTRNRAGNSPNVDLNVLVLSQSAWPTYDEVPVVIPLEMAQYLESYRNVYCEKHSGRKLMWRHALSHCVLRARFAPNVNKELVLSALQAVVLLLFNDAEFGTYLSYQQIKGGTGLDDKQLIRTLQSLACAKYRVLQKETKGKDILPTDNFCVNRHFSAPKFRIKINQIQLKETKKEKEDTFERVAQDRQYETQAAIIRIMKSRKKLRHNDLIQMTIDQTKNRGKLDVPEIKKQIERLIDKDYMERLPGGETWYQYVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHN5</accession>
    <name>D5GHN5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="13">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00008059001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleoporin GLFG family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430320">
        <property type="protein sequence ID" value="CAZ84065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839874.1">
        <property type="nucleotide sequence ID" value="XM_002839828.1"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GHN5"/>
    <dbReference type="MEROPS" id="S59.A07"/>
    <dbReference type="GeneID" id="9183719"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008059001"/>
    <dbReference type="eggNOG" id="KOG0845">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002330_0_0_1"/>
    <dbReference type="InParanoid" id="D5GHN5"/>
    <dbReference type="OMA" id="PMGKGLN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044614">
        <property type="term" value="C:nuclear pore cytoplasmic filaments"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008139">
        <property type="term" value="F:nuclear localization sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000973">
        <property type="term" value="P:post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006405">
        <property type="term" value="P:RNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034398">
        <property type="term" value="P:telomere tethering at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2360:FF:000001">
        <property type="entry name" value="Nuclear pore complex protein Nup98-Nup96"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1610.10:FF:000003">
        <property type="entry name" value="Nucleoporin SONB, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.690">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1610.10">
        <property type="entry name" value="Peptidase S59, nucleoporin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037665">
        <property type="entry name" value="Nucleoporin_S59-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007230">
        <property type="entry name" value="Nup98_auto-Pept-S59_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036903">
        <property type="entry name" value="Nup98_auto-Pept-S59_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021967">
        <property type="entry name" value="Nup98_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23198:SF6">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP98-NUP96"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23198">
        <property type="entry name" value="NUCLEOPORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04096">
        <property type="entry name" value="Nucleoporin2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12110">
        <property type="entry name" value="Nup96"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82215">
        <property type="entry name" value="C-terminal autoproteolytic domain of nucleoporin nup98"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51434">
        <property type="entry name" value="NUP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="6" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="9" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Peptidase S59" evidence="12">
        <location>
            <begin position="901"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="58"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="414"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="444"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="475"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="561"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="628"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="833"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1053"/>
            <end position="1098"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1127"/>
            <end position="1188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1298"/>
            <end position="1321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="27"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="71"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="414"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="424"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="481"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="614"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1053"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1143"/>
            <end position="1152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1168"/>
            <end position="1188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1310"/>
            <end position="1321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ84065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1995" mass="209977" checksum="4514E8A38DF659B1" modified="2010-06-15" version="1">MFGSGPSSGTGGGFGGFGTNNNNQTGTTGGSVFGSGTGFGQPAATGFGATNNAAASPFGTSKPFGGTTQPGTGGLFGGGNSSNTTAGGTGFGFGATNNTSNVFGGGNTAAAGGSGLFGNSKPAFGPTTTTAGATSGLFGTANSGTGGVFGGGANTGIGFSSGTAQGPLTGTANPPYTAFTEKEAQGGATNHFQTITFMPAYRNWSLEELRLQDYVQGRRYGTDSGNSGAFGQGTQFGAAFGATSGNNTSGFGPTNNSATTGGGLFGGAANNNTASTPFGAANTGGGFGTGATQTGSGGLFGASKPATGGLFGAPSNTTTTGGSGLFGAGANNTTTGAFGGSNTTGTFGATGATGATGGLFGAANNNNQQAQKPFSFGNTGTGFGGGASNTAFNAQGNNATTTGGGLFGNTQSTAPAFGTTQTGTGFGGTGGAFGTAGQNQGGAAFGGAFGNNPQAQPNKPFGTAFGATQATTATGGGGLFGNNNQQQQQQNTGGLFGAGDNKQFGPPAISTGAQGGGLFGNNATATGGTAGGLFGGGQATQASQGGLFGGGTGANAGSVGLFGNNANKPPGSQGGGLFTNNGPPSSTSGGLFGGSALGGSQLNTSTGGGFFNNQQQPQQQQQQIAGNGLFGSQFGNSLQGQQQQQQGFTASVMDSPYISPVLNSSLGNVPNSPMGPIATPLSSSVQTKKAAMIPHYKIAPRQPSLAPRLGNSFSRSGSPFAASSGSHATLGNSGSLGRSFSSTNKLHLFDGDDSILNSSSFTPNTSSRVASLKRLVIDKKIRDQDLFSGGQDLRKDNSGGSDFAAKGGTKSILKKTVSFDIAASRRGEEELYGAGATSAKGKETIGPTPTAEEMGFLRSTPERRRLREVEETSNGSSTSDAPVVGNEVAIISEKPKDDKSPGTYWMVPNASKLKILPKEQQKRVMNLTIGRRGYGQVRFDNPVDISDIEDIDKIPGHIVLFAQRVCTVYPETMPKPPPGKGLNVPATITLEDCFPVSKNERGKIRDPEHPRYVTHIRRLKSIKDTEFVNYLASEGMWIFRVRHFTTYGLVESDDEEEDGDYTVDEPTMLPTGETPTPAKTPGSSLWDGDEESSMDADVSGVDNTSSYDDTFDFKKFGSSAIVSGSKRDKHSTFLGRSDRETSYEESEEEGDVTMDQSFLGEGSVGSAEDVDEPAEPVSDQSEEEVDTTVVDETDGDVTGALVLANSDMLSPVKEYEEEDTITQDSPAKPLPMGQDWTEQLNNTISPKKRRFGGESFMASTCKLANNPAKKFNIEPMSYGLLDLANDLYGGRASTINGTSTKGKGRATWDQVESPTSSRYQHENAWTATKNGESDAGTAGSDEKRWRNAIKPVWGKDGSIIYFGDLEGLERRRNYGNDLKLAKLKYSIQNSESHGTPKPLELQLRATTIKRDEFNVPVAELRMDTLFHSFAQLSWSPDDPAGQHEKSVWELASVLWDPIAGELALEEESQEMIEYMQETCRKERLSRFLEALVEAAADEHVHGATTLEEVAIAHLTAHRVEQACASLLEAGDFRLANLITMVGGDAKLRSSIEKQLEQWKNKGVLAEIPIPIRALYELLAGNTCYSEGVRGPPEDAAPNFFILDRFNLDWKRGFGLRLWYGCADEEPIGKAVVDYETDMREYPEAVPAPKPWYHTEASPARDGAIDILWGLLKLYADRELNLEEVLAPMNMTTSYIDYRLSWQLRTILARKGIRDFSGGKLHRRIDGLDEFIAGGMADQTTIDFAGELETLGMWEWAIFVLLHLTDTDSRETAVRNVIGKHIDELKSAEGADVGRKLEFLEKTLLIPKNWICEARALQARSAGEHSLEAEYLLAAQAWNEAHKTVVKKVAPEAVISGHLEDLKSILAKFEDVDLVDKWNLGGQIYLDYIHLQEYVLSGKPHLKNAPEQKGLPKSNSPKDLARRLLAGLKNADRKGFLQNIAVREMAGVVGSWVLKSEDMISEAPKLLELPLTEDQFLRKTVNLGLAYYKARLQEAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHW3</accession>
    <name>D5GHW3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00008146001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430321">
        <property type="protein sequence ID" value="CAZ84106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839915.1">
        <property type="nucleotide sequence ID" value="XM_002839869.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHW3"/>
    <dbReference type="GeneID" id="9184199"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008146001"/>
    <dbReference type="eggNOG" id="ENOG502RYSB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_504428_0_0_1"/>
    <dbReference type="InParanoid" id="D5GHW3"/>
    <dbReference type="OMA" id="ASISYPM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054293">
        <property type="entry name" value="DUF7029"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055647">
        <property type="entry name" value="DUF7223"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22974">
        <property type="entry name" value="DUF7029"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23865">
        <property type="entry name" value="DUF7223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072088" evidence="2">
        <location>
            <begin position="20"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7029" evidence="3">
        <location>
            <begin position="108"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7223" evidence="4">
        <location>
            <begin position="287"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="567"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="575"/>
            <end position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ84106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="606" mass="63665" checksum="5CDC873EF8AE7F7D" modified="2010-06-15" version="1">MRTSAYTALLGGLLGLSSAAVIERSAVELKPIKPDAFVPGLTPRSEGEAPTVLRPINPDIFAAVNGLKPRADVDFSKLDLADQAQLIFGAPGEDGNVMLANMTLYAPSGQQIVMMESFEGLTSAVDCKGDDGEMALTFKSQEAFDYAHKQWSFINEKSENAFLLIANHDGCGPDDERQAYNITGIDDVDGTLTTLLKASPASWSDISGTFDLDFGYASLTPDAARRLRARGIGDFFDDITDTIGDGLDSIGDAFGSIGDAEFDKSVSFDVSVGTEGKRTDIIPKLFACVDCFITGKFKVAGKLSVDNFVVKDLTLGASPEDFRAKMIMEARVSATLLNGIPGLNLTQELASFPIPGAGISVPKIFELGAIITYEVGVECSVTGEANFTFGLSASLPNSALVSLNLADVSQSQASGFDGATFDPVFDINSGSVSLNVAAISRPKLTFGIEIIGVGKLEAALKMGLPVVSTKLTAAFDEQGVCEKTPTASRTGFKVNSDVSVNLAVSVDGSIGSVSTGLEKNLFTFKQPLLDKCFPLNIPALDPKPSQTTSALPSASATAASAKDTISGFKPGNLTLPGSNHSEPITNHTAPSPTGRKLPLRALRFSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5GI98</accession>
    <name>D5GI98_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00008354001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430324">
        <property type="protein sequence ID" value="CAZ84241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840050.1">
        <property type="nucleotide sequence ID" value="XM_002840004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GI98"/>
    <dbReference type="STRING" id="656061.D5GI98"/>
    <dbReference type="GeneID" id="9181959"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008354001"/>
    <dbReference type="eggNOG" id="KOG2190">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022670_4_2_1"/>
    <dbReference type="InParanoid" id="D5GI98"/>
    <dbReference type="OMA" id="TERSYFP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035925">
        <property type="term" value="F:mRNA 3'-UTR AU-rich region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070935">
        <property type="term" value="P:3'-UTR-mediated mRNA stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903138">
        <property type="term" value="P:negative regulation of cell integrity MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903753">
        <property type="term" value="P:negative regulation of p38MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd22455">
        <property type="entry name" value="KH-I_Rnc1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22456">
        <property type="entry name" value="KH-I_Rnc1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22457">
        <property type="entry name" value="KH-I_Rnc1_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049786">
        <property type="entry name" value="Rnc1_KH-I_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="50"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="134"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="298"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="217"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="20"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="30"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="232"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="269"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ84241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="386" mass="40944" checksum="363884A56EA09829" modified="2010-06-15" version="1">MSNEVADNHDGSPAGDESLFDNSFQNLNVNDDHHDGDHEGARTEEEYAQSPLTLRAIVSSKEAGVIIGKAGKNVADLREETGVKAGVSKVVTGIPDRVLTVTGPLDGVAKAYAMVSQTLLDSPPSTIGGGPPPGPGTLRLLISHNQMGTIIGRQGLKIKHIQDVSGTRMTAAKEMLPQSTERVVDVAGDPEAIRTAIWEIGKCLVDDWQRGTGTVLYNPAVRSPGGSSSVGMGNGMGGSDRYGGERGNTRSYARTGNGADFSDSSPPYRRHDGPSGRPSDRSPSSSNTQPMHTDDGEEIQTQNISIPADMVGCIIGRGGTKIQDIRRSSGARISIAKTPHGDTGERMFTIMGSASSNEKALYLLYENLEAEKLRRSNQGSPNHEIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIP6</accession>
    <name>D5GIP6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="13">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00008598001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in pre-mRNA splicing and cell cycle progression. Required for the spliceosome assembly and initiation of the DNA replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Associated with the spliceosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the crooked-neck family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430328">
        <property type="protein sequence ID" value="CAZ84389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840198.1">
        <property type="nucleotide sequence ID" value="XM_002840152.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIP6"/>
    <dbReference type="FunCoup" id="D5GIP6">
        <property type="interactions" value="1030"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GIP6"/>
    <dbReference type="GeneID" id="9183323"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008598001"/>
    <dbReference type="eggNOG" id="KOG1915">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011554_1_0_1"/>
    <dbReference type="InParanoid" id="D5GIP6"/>
    <dbReference type="OMA" id="HIKVWIS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
        <property type="term" value="P:spliceosomal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000048">
        <property type="entry name" value="Cell cycle control protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000306">
        <property type="entry name" value="Cell cycle control protein cwf4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000269">
        <property type="entry name" value="Crooked neck pre-mRNA-splicing factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055433">
        <property type="entry name" value="HAT_Syf1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055430">
        <property type="entry name" value="HAT_Syf1_CNRKL1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045075">
        <property type="entry name" value="Syf1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11246:SF3">
        <property type="entry name" value="CROOKED NECK-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11246">
        <property type="entry name" value="PRE-MRNA SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23231">
        <property type="entry name" value="HAT_Syf1_CNRKL1_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23233">
        <property type="entry name" value="HAT_Syf1_CNRKL1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Pre-mRNA-splicing factor Syf1-like N-terminal HAT-repeats" evidence="12">
        <location>
            <begin position="60"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="Pre-mRNA-splicing factor Syf1/CRNKL1-like C-terminal HAT-repeats" evidence="11">
        <location>
            <begin position="225"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="Pre-mRNA-splicing factor Syf1/CRNKL1-like C-terminal HAT-repeats" evidence="11">
        <location>
            <begin position="367"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="603"/>
            <end position="623"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF23233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ84389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="668" mass="80491" checksum="54DE407482B8868C" modified="2010-06-15" version="1">MEASRGPPRVKNKGASTQQISAEQILREAFERQEQGLQAPTQRFADLEELHEFQGRKRKEFEDYVRRNRINMNNWMRYAQWELDQKEYARARSIFERALDVDSRSVVLWLRYIEAEMKTRNINHARNLLDRAVTILPRVDKLWYKYVYMEETLGNIPGTRQVFERWMSWEPDEAAWSAYIKLEKRYDEFARARTIFQRFTQVHPEPRNWIKWARFEEEFGTEDNVREVYTLAVETLGEEFMDEKLFIAYARYEAKLKEYERARVIYQYALDRLPRSKSQLLHKSYTTFEKQFGEREGVEDVILSKRRVQYEEQIKENPKNYDVWFDYARLEETLGDKDRVRDVYERAIANIPPTKDKRHWRRYIYLWVFYALWEEMKGKDIDRTRQIYNECLNLIPHKRFTFAKIWLLKAHFEVRHFNLPAARKTLGQAIGACPKDKLFKGYISLETRLHEFSRCRTLYEKHIEFNPSNAQTWIRFAELEMALEDCDRVRAIFELAVDQELLDMPELLWKAYIDFEEEGGEFDKVRGLFERLLEKTDHVKVWISYAHFEVNADEGEDEDSVSEETKARAREIFERAYKRLKEKELKEERVALLNAWKAFEQTHGTPEDQKKVEAQMPSKVKKRRKLDDDTYEEYMEYMFPADEQSNARILNMLQAARAWKQNQAGLGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIY4</accession>
    <name>D5GIY4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00008737001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840286.1">
        <property type="nucleotide sequence ID" value="XM_002840240.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIY4"/>
    <dbReference type="STRING" id="656061.D5GIY4"/>
    <dbReference type="GeneID" id="9181818"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008737001"/>
    <dbReference type="eggNOG" id="ENOG502QVF9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009617_1_0_1"/>
    <dbReference type="InParanoid" id="D5GIY4"/>
    <dbReference type="OMA" id="PSHFIGN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051615">
        <property type="entry name" value="Transcr_Regulatory_Elem"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31313:SF79">
        <property type="entry name" value="C6 FINGER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31313">
        <property type="entry name" value="TY1 ENHANCER ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="9">
        <location>
            <begin position="39"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="79"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="117"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="682"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="826"/>
            <end position="856"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ84477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="856" mass="94504" checksum="68758440AC059752" modified="2010-06-15" version="1">MQQSINLSPTDLGGARENHRNYVFVDEHNRHKRLKVMRACGGCRRRKIKCDAATTNTWPCSACVRLKLHCVPPMVQYDGDFAPSQPPEQDGNGEFDNSSGSGEEEMIPGHLVARKQSRSALNYPPRDSHGPYRNISPPEQHHGTSHGAPPTIAFTGIHSGEGIEPPLSGMHFPAPVYQTQAVMRSQEQWPGDTEYVSTAHVSGVLGQLKIDDSGVANYISAQGRRLAETPAYEPWTEEIEPNPSFLPVQHDFAVRIHQEDMPTEDEAQEMFGIFFNHIHPYIPIVNKAAFYRQWNTSRDSISPLLLETIFACAGRLTTDPSKGLKWMGMATRHVDCFLDVPRLSTVQALLLLLKARESAPQRGYFFRSWMSVVSIIAMARDLGLDKHYELHKQGVGCGEGPLDCMTRTRVWQACFVYELMICAPQGRDIMQCNPDSIDLQTPIVSPDCDESEVAICESFIYFVWLVKNIRRMNDVHAKLKSTPNWRNDPQFLELWVRLDVWAYKLPAKLRVNVSTELGTSGPSGGLTFSGKISKIRSRWRVNGKPHMTICTNSAKSICRLEETVFEQYGMLGLQCMLRGVNFTIYTLLTCAMIHLIAATCPDPEFNHDAKEYFTRTMRILENCIDNSASSEVKGQIETLREAFSLDVNRPFVLNPNFPFYPGVSGPVDPSITRAYTQGESSTLATGPVNYPTHPLSPPHSAGGSEPKGESPAAVQSLVMLAAGQGAQNSTPIVDTLAWNPSRLFDNWNAAFGVNGTVSAAMPQAQMAAHESGLVDPLGGGRPPSSSSYVPTTVGVSHSFVSPGMWQDSVAAAAVFSDSHKRPFPFGGETVWHPSPSTDRDPVSPPTLHADAGRNSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJ44</accession>
    <name>D5GJ44_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008833001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the OBAP family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840346.1">
        <property type="nucleotide sequence ID" value="XM_002840300.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJ44"/>
    <dbReference type="GeneID" id="9181878"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008833001"/>
    <dbReference type="HOGENOM" id="CLU_2575589_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJ44"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010686">
        <property type="entry name" value="OBAP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31360:SF0">
        <property type="entry name" value="OIL BODY-ASSOCIATED PROTEIN 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06884">
        <property type="entry name" value="DUF1264"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="81" mass="8929" checksum="27C5D7A859F58ACD" modified="2010-06-15" version="1">MEELAKLYGKTYHLWQVDRGDEVPLGAPMLMGSFLKETKGVEMLGSIFSSRGFIRIRINGITASILRSHFGSDPTSGLESP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJ82</accession>
    <name>D5GJ82_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">2-isopropylmalate synthase</fullName>
            <ecNumber evidence="7">2.3.3.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">GSTUM_00008878001</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3-methyl-2-oxobutanoate + acetyl-CoA + H2O = (2S)-2-isopropylmalate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21524"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.13" evidence="7"/>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840384.1">
        <property type="nucleotide sequence ID" value="XM_002840338.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJ82"/>
    <dbReference type="FunCoup" id="D5GJ82">
        <property type="interactions" value="167"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJ82"/>
    <dbReference type="GeneID" id="9185943"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008878001"/>
    <dbReference type="eggNOG" id="KOG2367">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004588_3_0_1"/>
    <dbReference type="InParanoid" id="D5GJ82"/>
    <dbReference type="OMA" id="WPDKVID"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003852">
        <property type="term" value="F:2-isopropylmalate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07942">
        <property type="entry name" value="DRE_TIM_LeuA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000045">
        <property type="entry name" value="2-isopropylmalate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.270:FF:000002">
        <property type="entry name" value="2-isopropylmalate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00572">
        <property type="entry name" value="LeuA_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013709">
        <property type="entry name" value="2-isopropylmalate_synth_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002034">
        <property type="entry name" value="AIPM/Hcit_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005668">
        <property type="entry name" value="IPM_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054692">
        <property type="entry name" value="LeuA-like_post-cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036230">
        <property type="entry name" value="LeuA_allosteric_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039371">
        <property type="entry name" value="LeuA_N_DRE-TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00970">
        <property type="entry name" value="leuA_yeast"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002991">
        <property type="entry name" value="PRK03739.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46911">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46911:SF1">
        <property type="entry name" value="2-ISOPROPYLMALATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22615">
        <property type="entry name" value="IPMS_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08502">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00917">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110921">
        <property type="entry name" value="2-isopropylmalate synthase LeuA, allosteric (dimerisation) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89000">
        <property type="entry name" value="post-HMGL domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00815">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00816">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="13" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0432">Leucine biosynthesis</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="14">
        <location>
            <begin position="33"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAZ84575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="619" mass="68375" checksum="40AA09D69445B467" modified="2010-06-15" version="1">MPMLKDPSKKYQAFKPVHLPDRQWPSKTLDKAPRWLSTDLRDGNQSLIDPMDGDQKWRYFKMLTDIGYKEIEVSFPSASQTDFDFTRRLIETPGAIPDDVWLQVLSPCREDFIRRTIASLKGANKAIVHLYLATSECFRRIVFGMTEEQSVELAVKSTKLVRSLTKDNPNAQGTEWAYEFSPETFSDTSLEFAVKICEAVKAAWEPTKENPIIFNLPATVEMSTPNVYADQIEYFCRHISEREKVCISLHPHNDRGCATAASELAQMAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGIHPNVDFSDITNIINVVEDCNKIPVHPRAPYGGQLVVCAFSGSHQDAIRKGFKLREKAHAADDDEWQIPYLPLDPADIGRTYEAVIRVNSQSGKGGVAWVIQRALDLDLPRGLQIVFSKIVQKETEDLGRELEAGEIQALFETEYHLKSAPRITLIDYEFSTDRSTSPNPVLGKQSANAKRTFHGIISVDGRERSIRGVGNGPISSLADALRTVGVELDVLDYKEHAIGRGKSVKAATFIECDISGSAEAEKRVVWGVGIHEDTAQASLIAILGAASKAISSRPATSMLTASLTSPLTTLKPSSVKINFNGLESKAEGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJ91</accession>
    <name>D5GJ91_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Casein kinase II subunit alpha</fullName>
            <shortName evidence="10">CK II alpha</shortName>
            <ecNumber evidence="10">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00008887001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Heterotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="10"/>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840393.1">
        <property type="nucleotide sequence ID" value="XM_002840347.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJ91"/>
    <dbReference type="FunCoup" id="D5GJ91">
        <property type="interactions" value="929"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJ91"/>
    <dbReference type="GeneID" id="9185952"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008887001"/>
    <dbReference type="eggNOG" id="KOG0668">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_70_4_1"/>
    <dbReference type="InParanoid" id="D5GJ91"/>
    <dbReference type="OMA" id="ECHMIEW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005956">
        <property type="term" value="C:protein kinase CK2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006356">
        <property type="term" value="P:regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006359">
        <property type="term" value="P:regulation of transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14132">
        <property type="entry name" value="STKc_CK2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000059">
        <property type="entry name" value="Casein kinase II subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000088">
        <property type="entry name" value="Casein kinase II subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045216">
        <property type="entry name" value="CK2_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24054">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24054:SF0">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 9" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="32"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU369118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ84584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="335" mass="39450" checksum="92A587BBA0AE6A48" modified="2010-06-15" version="1">MARVYADVNQHMPRTYWDYDSVNIPWGVLENYEVVRKIGRGKYSEVFEGINITNYQKTVIKVLKPVKKKKIKREIKILQNLSGGPNIVALLDVVRDPQSKTPSLIFEYINNTDFRSLYPKFQDYDVRYYIYELLKALDFCHSKGIMHRDVKPHNVMIDHEKRKLRLIDWGLAEFYHAGTEYNVRVASRYFKGPELLVDYQEYDYSLDLWSLGAMFASMIFRKEPFFHGNSNSDQLVKIAKVLGTEELFDYLDKYDIELDTQYDDILGRFPKKSWHQFVNADNQRFVSNDALDFLDKLLRYDHQERLTAKEAMAHPYFEPVRRAASTPNHGSSSHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJB5</accession>
    <name>D5GJB5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GTP-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00008924001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">GTPase involved in activation of the TORC1 signaling pathway, which promotes growth and represses autophagy in nutrient-rich conditions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the GSE complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the GTR/RAG GTP-binding protein family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430330">
        <property type="protein sequence ID" value="CAZ84608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840417.1">
        <property type="nucleotide sequence ID" value="XM_002840371.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJB5"/>
    <dbReference type="FunCoup" id="D5GJB5">
        <property type="interactions" value="569"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJB5"/>
    <dbReference type="GeneID" id="9185976"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008924001"/>
    <dbReference type="eggNOG" id="KOG3887">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047421_2_0_1"/>
    <dbReference type="InParanoid" id="D5GJB5"/>
    <dbReference type="OMA" id="NCRTFQE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990131">
        <property type="term" value="C:Gtr1-Gtr2 GTPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010507">
        <property type="term" value="P:negative regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11385">
        <property type="entry name" value="RagC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001086">
        <property type="entry name" value="GTP-binding protein GTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006762">
        <property type="entry name" value="Gtr1_RagA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039400">
        <property type="entry name" value="RagC/D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11259:SF2">
        <property type="entry name" value="GH16429P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11259">
        <property type="entry name" value="RAS-RELATED GTP BINDING RAG/GTR YEAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04670">
        <property type="entry name" value="Gtr1_RagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ84608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="285" mass="32819" checksum="7D85A89BEAC62F68" modified="2010-06-15" version="1">MPPTETLFLESTTRIQKDPIHSFMDFQVWDFPGQLNFLDPTFDFDHIFGELGALIWVIDAQDEYLEPLSKLHTTIIAIQRFYPAINIEVFVHKVDGLSDEYKLDTQRDIMQRTQDELSDNGFDNVQVSYHLTSIYDHSIFEAFSKVIQKLIPQLGTLENLLNILCSNSGIEKAFLFDVLSKIYIATDSSPVDMQSYEICSDYIDVIVDVSEIYGYLVDPLQEEATVEASSIIRLQNGMVLYLREINKYLALICLMRKDSIEKQGLVDFNVQVFQNALQRVFEVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJE2</accession>
    <name>D5GJE2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mediator of RNA polymerase II transcription subunit 8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Mediator complex subunit 8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">MED8</name>
        <name evidence="2" type="ORF">GSTUM_00008958001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Mediator complex subunit 8 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430330">
        <property type="protein sequence ID" value="CAZ84635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840444.1">
        <property type="nucleotide sequence ID" value="XM_002840398.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJE2"/>
    <dbReference type="FunCoup" id="D5GJE2">
        <property type="interactions" value="130"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJE2"/>
    <dbReference type="GeneID" id="9182636"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008958001"/>
    <dbReference type="eggNOG" id="ENOG502S8U1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_987620_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJE2"/>
    <dbReference type="OMA" id="PQWYSLQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019364">
        <property type="entry name" value="Mediatior_Med8_fun/met"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10232">
        <property type="entry name" value="Med8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0010">Activator</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU364144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="282" mass="31838" checksum="4E10B41462284AF3" modified="2010-06-15" version="1">MFRDLGEASKRAYLHIIIPEVAHSSSIQFHHCLHPHLRSHHHHHHFPPTLALALPTPPLRIGKIYSFPAHYGVMENPQRSLESTRARFNRLVSALTAFDSALRTGTPEQLPPWQTLQPHFNVLFSILDSLSHTLNQQFPPPQINTSALVSYPLPSFPANSQIQLSISTLLNKKLNPIVQDWALRALNAPTVNNDDLWDRAREIVQEQQEERNWYDDIYSLEEQEAGTEGIETGVSEEKQEKEEAIPGLKLEAILRFMETGVVPADQPALPMAAMPGTGPMKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJV3</accession>
    <name>D5GJV3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00009214001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430334">
        <property type="protein sequence ID" value="CAZ84796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840605.1">
        <property type="nucleotide sequence ID" value="XM_002840559.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJV3"/>
    <dbReference type="STRING" id="656061.D5GJV3"/>
    <dbReference type="GeneID" id="9186832"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009214001"/>
    <dbReference type="eggNOG" id="KOG0101">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009958_6_1_1"/>
    <dbReference type="InParanoid" id="D5GJV3"/>
    <dbReference type="OMA" id="IYKHTME"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10170">
        <property type="entry name" value="ASKHA_NBD_HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14187">
        <property type="entry name" value="ALPHA KINASE/ELONGATION FACTOR 2 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14187:SF82">
        <property type="entry name" value="FAMILY CHAPERONE, PUTATIVE (AFU_ORTHOLOGUE AFUA_7G08575)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="88"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ84796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="626" mass="68268" checksum="AF10B2324101ABDD" modified="2010-06-15" version="1">MAAEAMANLTLRSNSLNSIRTPTSSSSSTASAGKDRLVIGVDFGTTYSGVAAVYSGTPDDIEVIKTWPGGNGITSDKVPTELSYIPLPSRTPSGAGMPQPPVSHEGGSSDPVTYKWGFQPSANNPRLRCLKLFLDRSQTLPPYISPLETAGMLRGVGKTIIHAVTDYLSALYGESFMAGTPLEWVLTVPAVWSDAAKNATRRAAEGAGMGGPDGKGIRLISEPEAAAVYTLKAIQPNNLSVGDNFVVCDAGGGTVDLISYEITSVSPSLLLSESSIGTGGLCGSAFLNYAFEDHCRRRLGAEIYDHLKPKTRNMALKYWDEYVKRNFKDEEPDEEEEISVPLPGVADDEEKRIEGGFLVMRREEVKTIFEPVVSQVVKLVDEQVEGIRRKGGKVAGIVLVGGFGQSNYLYSRLKTRFNPPTLNPPSYSETPISGNGGIEVMQPLYAWTAVVRGAVIRGLEGSMVVARQARFHYGTSYATVWEESKHPVQDRYWSPLWERWMVSDRMQWHIARGEPVSDKTPISFHYTRNFRPGQSLKIEDDLICCEHEVAPASMADANKHLVKTVCTLTTDLSSVPKSKFTRLTTSKGIAFDNLDFTLDLIVDSASLVFELKVEGVAYGSVTAKFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJW8</accession>
    <name>D5GJW8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00009247001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430334">
        <property type="protein sequence ID" value="CAZ84811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840620.1">
        <property type="nucleotide sequence ID" value="XM_002840574.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJW8"/>
    <dbReference type="GeneID" id="9186847"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009247001"/>
    <dbReference type="HOGENOM" id="CLU_2238584_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJW8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="105" mass="11912" checksum="0DBC421D22D04725" modified="2010-06-15" version="1">MLSSTERTLVKDRKKNTSYQERKSEHTSSSYRQYLIARTAYLPILDLTQNRAAPPFTLQNFPVAKVLVPSSFQTPSPAQSRSSKLSRALFLHTVSRQTSLKPSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>D5GK33</accession>
    <name>D5GK33_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00009341001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the chaperonin (HSP60) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430335">
        <property type="protein sequence ID" value="CAZ84876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840685.1">
        <property type="nucleotide sequence ID" value="XM_002840639.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GK33"/>
    <dbReference type="SMR" id="D5GK33"/>
    <dbReference type="FunCoup" id="D5GK33">
        <property type="interactions" value="1334"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GK33"/>
    <dbReference type="GeneID" id="9186913"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009341001"/>
    <dbReference type="eggNOG" id="KOG0356">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016503_3_0_1"/>
    <dbReference type="InParanoid" id="D5GK33"/>
    <dbReference type="OMA" id="TDTDKME"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006458">
        <property type="term" value="P:'de novo' protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051131">
        <property type="term" value="P:chaperone-mediated protein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045041">
        <property type="term" value="P:protein import into mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03344">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000001">
        <property type="entry name" value="60 kDa chaperonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000001">
        <property type="entry name" value="60 kDa chaperonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00600">
        <property type="entry name" value="CH60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018370">
        <property type="entry name" value="Chaperonin_Cpn60_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001844">
        <property type="entry name" value="Cpn60/GroEL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02348">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000592">
        <property type="entry name" value="PRK00013.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009487">
        <property type="entry name" value="PRK12849.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009488">
        <property type="entry name" value="PRK12850.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009489">
        <property type="entry name" value="PRK12851.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45633">
        <property type="entry name" value="60 KDA HEAT SHOCK PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00298">
        <property type="entry name" value="CHAPERONIN60"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00296">
        <property type="entry name" value="CHAPERONINS_CPN60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ84876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="592" mass="62160" checksum="8FB84B544954AEC3" modified="2010-06-15" version="1">MQRALSSAVRVRPSFSTARPLALASSLMSSHQQSRAYSHKELKFGVEGRQSLLAGVDMLAKAVAVTLGPKGRSVLIESSYGSPKITKDGVTVAKAITLKDKFENLGARLLQDVASKTNEVAGDGTTTATVLARAIFTETVKNVAAGCNPMDLRRGTQAAVEAVVDYLQKNKRNITTSEEIAQVATISANGDQHIGNLIAQAMERVGKEGVITVKEGKTIEDELEVTEGMKFDRGYVSPYFITDTKTQKVEYEKPLILLSEKKISAVQDIIPALEASTTMRRPLVIIAEDIDGEALAVCVLNKLRGQLQVVAVKAPGFGDNRKSILGDIGVLTNATVFTDELDVKLEKLTSDMLGSTGSITITKEDTIILNGEGSKDIIAQRCEQIRGVMNDSTTSEYEKEKLQERLAKLSGGVAVIKVGGASEVEVGEKKDRFVDALNATRAAVEEGILPGGGTALLKASTNALGNVKVNNFDQQLGLSIVKSAITRPARTIVENAGGEGSVVVGKLTGEYGGDFNMGYDASGGEYVNMIEAGIVDPLKVVRTALGDASGVASLLGTTECTIVDAPEEKGPPGGGMGGGMGGMGGMGGMGGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GK49</accession>
    <name>D5GK49_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribose-5-phosphate isomerase</fullName>
            <ecNumber evidence="4">5.3.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">D-ribose-5-phosphate ketol-isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Phosphoriboisomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00009363001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>aldehydo-D-ribose 5-phosphate = D-ribulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:14657"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="EC" id="5.3.1.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate degradation; pentose phosphate pathway; D-ribose 5-phosphate from D-ribulose 5-phosphate (non-oxidative stage): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ribose 5-phosphate isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.6" evidence="4"/>
    <dbReference type="EMBL" id="FN430335">
        <property type="protein sequence ID" value="CAZ84892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840701.1">
        <property type="nucleotide sequence ID" value="XM_002840655.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GK49"/>
    <dbReference type="STRING" id="656061.D5GK49"/>
    <dbReference type="GeneID" id="9186929"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009363001"/>
    <dbReference type="eggNOG" id="KOG3075">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_332088_0_0_1"/>
    <dbReference type="InParanoid" id="D5GK49"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00412"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004751">
        <property type="term" value="F:ribose-5-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006014">
        <property type="term" value="P:D-ribose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009052">
        <property type="term" value="P:pentose-phosphate shunt, non-oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01398">
        <property type="entry name" value="RPI_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1360:FF:000014">
        <property type="entry name" value="Ribose 5-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004788">
        <property type="entry name" value="Ribose5P_isomerase_type_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014751">
        <property type="entry name" value="XRCC4-like_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00021">
        <property type="entry name" value="rpiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11934">
        <property type="entry name" value="RIBOSE-5-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11934:SF0">
        <property type="entry name" value="RIBOSE-5-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06026">
        <property type="entry name" value="Rib_5-P_isom_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75445">
        <property type="entry name" value="D-ribose-5-phosphate isomerase (RpiA), lid domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58022">
        <property type="entry name" value="XRCC4, C-terminal oligomerization domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="373"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="424"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="652"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="595"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="373"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="684"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="716"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="731"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="748"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="782"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="837"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="850"/>
            <end position="862"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ84892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="862" mass="93296" checksum="9D8853BB0FF61CAB" modified="2010-06-15" version="1">MTTTLSVVESAKRQAAYRAVDEHFPQHARFVGIGSGSTVIYVVERIVHLGRDVTDSITFIPTGYQSKELIINGGLKLGSIDRHTELDIAFDGADEVDPYLNCIKGGGACLFQEKLVAICAKKFVVVADYRKLSPSLSTHYAPGIPIEVVPPAATHILSRLITLGAVNPAIRMGGTSKAGPCITDNSNFIIDAPFPDGFIPSPSSPLSHPHKPLSPALSATPVNAAAVERGGERVGGMDILGKEGGHPGAAVGMGVGSNEKVEELARALKSIVGVVEHGIFWNGDRKPVVAYFGMEDGRVETRGCLFLFSFDIQTPQALNPIIPSCYGSDVEDALPEKKFHMCTNISPGTLEYSTSTGLYPAVWIFEATQSTEPTTQNQWTQSKTPHQITTSHPKDDDTAKPMHACLLQVNLAWIQNRPKTAHQPTTGRSCSFFPPTQQPIPNMSSSSSRRPTLTRLTRYDTPDADQFVLLHATPTSAVRPLDLKLHATEGERAFATNITQSKIYELRARSYTGTSEDLEKIFLALLLKQDVGVERGVELAAAVDAKGVTLTVRQDIGGIKQRIAALQIPEGEVEIPIFEWAVEACGDLNSGSTQLGSLQRKVESQEKEIEALTKQLKDLAVFKKEHEEMLLVKFSELLNSKKTKIRELSRELEVRGGDAVSSKAESPIAEDESEVAEPAPTRGRGGRGRARGRAKAAAPPRKRKPIAPPTDSESDSFAKMDTGSKGRPSPDDETEDSEEEAGPSRGYLRQDARSDRSITEDEDELPPVRRPIAFTIKKPDPEPEWTPDEDVEMDEPLPAQPARIGGRTKSGGGKGKKPAPPPTSRGTGRVTRSKPQEPSPSPPPSPPANDNDETGDSEDEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5GK61</accession>
    <name>D5GK61_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">GSTUM_00009382001</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">ATP-dependent RNA helicase associated with the nuclear pore complex and essential for mRNA export from the nucleus. May participate in a terminal step of mRNA export through the removal of proteins that accompany mRNA through the nucleopore complex. May also be involved in early transcription.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">Associates with the nuclear pore complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the DEAD box helicase family. DDX19/DBP5 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="3"/>
    <dbReference type="EMBL" id="FN430336">
        <property type="protein sequence ID" value="CAZ84904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840713.1">
        <property type="nucleotide sequence ID" value="XM_002840667.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GK61"/>
    <dbReference type="FunCoup" id="D5GK61">
        <property type="interactions" value="735"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GK61"/>
    <dbReference type="GeneID" id="9186942"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009382001"/>
    <dbReference type="eggNOG" id="KOG0332">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_1_0_1"/>
    <dbReference type="InParanoid" id="D5GK61"/>
    <dbReference type="OMA" id="HTRKMAG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17963">
        <property type="entry name" value="DEADc_DDX19_DDX25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000849">
        <property type="entry name" value="ATP-dependent RNA helicase DBP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="11" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="10" id="KW-0811">Translocation</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="20">
        <location>
            <begin position="69"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="18">
        <location>
            <begin position="102"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="19">
        <location>
            <begin position="280"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="16">
        <location>
            <begin position="69"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="CAZ84904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="453" mass="50581" checksum="C8C651F5CDBDDA19" modified="2010-06-15" version="1">MKEYTVSRKSRMGKKSKGPVGTAIATHKRSPSTPPPTTGGSGMNEPKYEVAVRFDDLQGDLYSPFYSVKRFEDLGLSKDLLEGIYFMNFKKPSMIQERALPLLLSNPPRNMIGQSQSGTGKTAALVLTMLTRIDMSVSNVEALCLAPTRELAKQIQRVVQTMGQFTNVKAQFAIPNMAKCSQRIDAHIVVGTPGTVLDLIQRNQLAVEHLKLFMLDEVDNMLELQGLGEQCLRVKRNIPSTTQIALFSATCSDEAFKYMYRFAPNANRIILKNTEPSLAGIKQLCMDCQSEEDKYRVLLELYHVLTVGSSIIFAKKRETTSEIQRRIEVDEHKVATLHSAQDRPDRDKAVHSFLSGKAKVLITTDVLVRGIDVATVSMVVNYDLPLDMNRQPDPVAYLHRVGRTGIFGRPGLSVNFVYDKHSLHQVTEISSYFGTCMTRVSTNNTDVCSFQTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKG5</accession>
    <name>D5GKG5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00009537001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430338">
        <property type="protein sequence ID" value="CAZ85008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840817.1">
        <property type="nucleotide sequence ID" value="XM_002840771.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKG5"/>
    <dbReference type="GeneID" id="9182028"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009537001"/>
    <dbReference type="HOGENOM" id="CLU_2759651_0_0_1"/>
    <dbReference type="InParanoid" id="D5GKG5"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="70" mass="8425" checksum="FD8989FFC420453C" modified="2010-06-15" version="1">MHIRRLIDLARYPRRPPPPPLRIKKWRPVSSANGEFPFTSLTPHHSEHTNHHPRYPDHLPFSQEERVIFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKI8</accession>
    <name>D5GKI8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">sphingolipid C(9)-methyltransferase</fullName>
            <ecNumber evidence="14">2.1.1.317</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">GSTUM_00009583001</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">Mel28</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CFA/CMAS family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.317" evidence="14"/>
    <dbReference type="EMBL" id="FN430339">
        <property type="protein sequence ID" value="CAZ85031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840840.1">
        <property type="nucleotide sequence ID" value="XM_002840794.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKI8"/>
    <dbReference type="STRING" id="656061.D5GKI8"/>
    <dbReference type="GeneID" id="9184692"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009583001"/>
    <dbReference type="eggNOG" id="ENOG502QS47">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026434_5_0_1"/>
    <dbReference type="InParanoid" id="D5GKI8"/>
    <dbReference type="OMA" id="GFKTWLF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006665">
        <property type="term" value="P:sphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052290">
        <property type="entry name" value="Sphingo_C9-MT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45197:SF1">
        <property type="entry name" value="SPHINGOLIPID C9-METHYLTRANSFERASE A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45197">
        <property type="entry name" value="SYNTHASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_7G04190)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02353">
        <property type="entry name" value="CMAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="10" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="52"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAZ85031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="500" mass="56980" checksum="1A4EE70463E560B7" modified="2010-06-15" version="1">MGGSQSEDSFEFVDTPPASTPQPASDSCGVRTTDYPAIPNAPLPAEGSGNDSWSNMLMFGLLLGVPYYLKYKVGGGFKTFLFFALIVDLPILVGFWVVMSTYSPRKNEKVKLPGKGVEHYLQFHKQEDAVKYKGGTKIPMEVFYEMYFEGDVSFKGDCLEILEHRHDWANFQFTKGLFKHFLLGMIPELLVHSRSQDEEQVRDHYDRGDDFYGWFLGPRMIYTSGLISDIHKEESLEQLQDNKLKVVCEKIDLKNGDRMLDLGCGWGTLARFASKSYGAHVTGITLGRNQTAWGNSGLRRDGIKESQSKILCMDYRDTPVPAGKYNKITCLEMAEHVGVRHFGAFLNQVYNMLEDDGTLFLQIAGLRKSWQFEDLIWGLFMNKYVFPGADASTPLGWFVDKLEGAGFEVKSVDTIGVHYSATLWRWYRNWMANREKVEAKYGNRWFRIWEFFLAYSSIISRQGSATCYQIVLHKNLNAFHRVEGISTQYQLSGALSVKRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKL7</accession>
    <name>D5GKL7_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Vacuolar protein sorting/targeting protein 10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Carboxypeptidase Y receptor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14 16">Sortilin VPS10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">GSTUM_00009633001</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Functions as a sorting receptor in the Golgi compartment required for the intracellular sorting and delivery of soluble vacuolar proteins, like carboxypeptidase Y (CPY) and proteinase A. Executes multiple rounds of sorting by cycling between the late Golgi and a prevacuolar endosome-like compartment.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Prevacuolar compartment membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430340">
        <property type="protein sequence ID" value="CAZ85060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840869.1">
        <property type="nucleotide sequence ID" value="XM_002840823.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5GKL7">
        <property type="interactions" value="174"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GKL7"/>
    <dbReference type="GeneID" id="9184722"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009633001"/>
    <dbReference type="eggNOG" id="KOG3511">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000700_0_0_1"/>
    <dbReference type="InParanoid" id="D5GKL7"/>
    <dbReference type="OMA" id="ATMSEFI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15482">
        <property type="entry name" value="Sialidase_non-viral"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.270:FF:000005">
        <property type="entry name" value="Sortilin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.80">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031777">
        <property type="entry name" value="Sortilin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031778">
        <property type="entry name" value="Sortilin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006581">
        <property type="entry name" value="VPS10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050310">
        <property type="entry name" value="VPS10-sortilin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12106">
        <property type="entry name" value="SORTILIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12106:SF27">
        <property type="entry name" value="SORTILIN-RELATED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15902">
        <property type="entry name" value="Sortilin-Vps10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15901">
        <property type="entry name" value="Sortilin_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00602">
        <property type="entry name" value="VPS10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110296">
        <property type="entry name" value="Oligoxyloglucan reducing end-specific cellobiohydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="18" id="KW-0732">Signal</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072843" description="Vacuolar protein sorting/targeting protein 10" evidence="18">
        <location>
            <begin position="21"/>
            <end position="1472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1358"/>
            <end position="1379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1405"/>
            <end position="1431"/>
        </location>
    </feature>
    <feature type="domain" description="VPS10" evidence="19">
        <location>
            <begin position="46"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="domain" description="VPS10" evidence="19">
        <location>
            <begin position="712"/>
            <end position="1346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00031902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CAZ85060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1472" mass="166141" checksum="0EF045473F4C2EE2" modified="2010-06-15" version="1">MLLLRVWALAAAWLATATLAKKSPEISKTKLENLPSIFFYFDDSPVILTLDAHANNVWRSDDDGNSWHKVKGVPDGAAWDITEHPFDKKRAFIFGEDKEHWYTEDRGAKWHKFKSEVPLSLLQPPLVFHAGQPEWILYSGRVCEDDDDYFGRSCKEVTYYTKDGFDSEMKKLKEETHGCMFARGTPIFEGAKDETVLCIVNGDTAYTENRRLLVSHNWFRDSEEPKLDGYKTVNGLAGLAGIQKFIVAAVKTRGTDEMALYVTKDTKTWHRAEFPHDHGGLREDAYTVLESTPYSIQVDVLTTRAMNPMGSLFTSNSNGTYFTRNLDHTNRAVEGLVDFEQVANIQGIVLANVVNNADRVLENPTIRKERQSVISFDDGKWGTWVPLKGPDGKRLNLYSVTALADGGRIYSSPAPGILMGVGSLGDHLAGYKDCDLFYSDDAGLTWRKALTDAHKYEIGGSGSVMVAINDEDMTDIVSYSIDHGKNWEEVKLDERIRARLLTTTPDSTTLKFTLIGTARDKTPYVFGLDFSEVFERKCKLDKSSEDGDYEKWYARLDEEGKPDCLMGHKQYFWRRKPGVNCYVGEDYKDPEPEKEVCECTKHDFECDFNFVRENRGKDDDCVPAKGVKFSIPAGECKKDEDTYMGPSGYRKIPGNDCKGGESLEDKVERKCNEVTTTPSNGKVTDVKTSFAGKRVAEYFYLENAESASKSDETIIMRTEEQEVWITRDHGATWNRILEKVNVVSIYPNPNLWDSVYFITPDAEVWYTLDRGEHIKKMASVKTKPNRQGLQVIDFHPTKQGWLIWTGQEDCDSPSTCHSVAYHTKDNGEEWHRLLAYVGTCKWIRTEKQSEKLIFCEHFETERGNGEGNLELVASDNWFDDRHVKFRSIIGYATMAEFIIVASINEDGTSLKASASVNGSIFAEAAFPHNFNVPHQTAYTVLDSETHAVFLHVTVNPRKGFEFGSLLKSNSNGTNYVLSLDAVNRNEEGFVDFEKLKTLEGVAIANRVVNADKAVDGEKKRLRSMITHNDGATWDYILAPEKDADGKDHKICDTSDKKKCSLNLHHYTDRVDPRDTFGSGSAVGLMMGIGNIGDVLTGYKDGDTYLTRDGGVRWKQVKKGQYQWEYGDQGSIIVLVEDNVLTDRVHYSLDEGESWTEYTFAGSSDEKMRVADISTLPSDTSRKFILWGTREGDGNKFWTVQLDFTGLTDVKCDLKDGDGADSDDFELWSPEHQDGCLFGHRVQYNRKIRTHNCYIGRKIKQPHKILEKCKCTRHDYECDFNYQRDKDGACYLVEGLTPPDHQAVCKENKDQDEYWEPTGYRRIPMTTCEGGQELDKSISHYCPGHKKEWEKKHGGARGFALFLAIIAPITAAAVIGWFVWTRLLDGRFGAIRLGEDNNGQSPFVRYPIIVISAIVALAVSIPTILGAIGSWASSKLTRTRRYTSRSSFARGADYSVVNTDEGELLGSDDEDEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKX0</accession>
    <name>D5GKX0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00009804001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the RecA family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430341">
        <property type="protein sequence ID" value="CAZ85163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840972.1">
        <property type="nucleotide sequence ID" value="XM_002840926.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKX0"/>
    <dbReference type="FunCoup" id="D5GKX0">
        <property type="interactions" value="129"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GKX0"/>
    <dbReference type="GeneID" id="9183399"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009804001"/>
    <dbReference type="eggNOG" id="KOG1434">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041732_2_1_1"/>
    <dbReference type="InParanoid" id="D5GKX0"/>
    <dbReference type="OMA" id="QQIECIT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000794">
        <property type="term" value="C:condensed nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070192">
        <property type="term" value="P:chromosome organization involved in meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000730">
        <property type="term" value="P:DNA recombinase assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042148">
        <property type="term" value="P:DNA strand invasion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006312">
        <property type="term" value="P:mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002052">
        <property type="entry name" value="DNA repair protein RAD51 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013632">
        <property type="entry name" value="Rad51_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020587">
        <property type="entry name" value="RecA_monomer-monomer_interface"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22942:SF30">
        <property type="entry name" value="MEIOTIC RECOMBINATION PROTEIN DMC1_LIM15 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22942">
        <property type="entry name" value="RECA/RAD51/RADA DNA STRAND-PAIRING FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08423">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50163">
        <property type="entry name" value="RECA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecA family profile 1" evidence="5">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="RecA family profile 2" evidence="6">
        <location>
            <begin position="165"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ85163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="217" mass="23701" checksum="07C54AFE60810343" modified="2010-06-15" version="1">MSITEVFGEFRTGKSQLSMTVSVICQLPRDHGGAEGKVAYIDTEGTFRPERIKQISDRFGVDGDAACENIIYGRALNSEHQLELLNGLCQNFASGEFRLLIVDSIMACFRVDYCGRGELSERQQKLGQMLSKLSHMAEEFNVAVLMTNQVQSDPGASALFAGADGHVLAHASATRILLRKGRGEERVAKLQDSPDCPEREATYIITNGGISDPEGTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLH4</accession>
    <name>D5GLH4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Endonuclease</fullName>
            <ecNumber evidence="17">3.1.30.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">GSTUM_00010203001</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2 17">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the DNA/RNA non-specific endonuclease family.</text>
    </comment>
    <dbReference type="EC" id="3.1.30.-" evidence="17"/>
    <dbReference type="EMBL" id="FN430349">
        <property type="protein sequence ID" value="CAZ85367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841176.1">
        <property type="nucleotide sequence ID" value="XM_002841130.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLH4"/>
    <dbReference type="FunCoup" id="D5GLH4">
        <property type="interactions" value="223"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GLH4"/>
    <dbReference type="GeneID" id="9186111"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010203001"/>
    <dbReference type="eggNOG" id="KOG3721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055174_0_2_1"/>
    <dbReference type="InParanoid" id="D5GLH4"/>
    <dbReference type="OMA" id="YVMPNQV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000014">
        <property type="term" value="F:single-stranded DNA endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006309">
        <property type="term" value="P:apoptotic DNA fragmentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00091">
        <property type="entry name" value="NUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.570.10:FF:000004">
        <property type="entry name" value="Nuclease 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.570.10">
        <property type="entry name" value="Extracellular Endonuclease, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018524">
        <property type="entry name" value="DNA/RNA_endonuclease_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044929">
        <property type="entry name" value="DNA/RNA_non-sp_Endonuclease_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001604">
        <property type="entry name" value="Endo_G_ENPP1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020821">
        <property type="entry name" value="ENPP1-3/EXOG-like_nuc-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044925">
        <property type="entry name" value="His-Me_finger_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040255">
        <property type="entry name" value="Non-specific_endonuclease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13966:SF5">
        <property type="entry name" value="ENDONUCLEASE G, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13966">
        <property type="entry name" value="ENDONUCLEASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01223">
        <property type="entry name" value="Endonuclease_NS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00892">
        <property type="entry name" value="Endonuclease_NS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00477">
        <property type="entry name" value="NUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54060">
        <property type="entry name" value="His-Me finger endonucleases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01070">
        <property type="entry name" value="NUCLEASE_NON_SPEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 17" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="10 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0464">Manganese</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 16 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="6 17" id="KW-0540">Nuclease</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="18" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072201" description="Endonuclease" evidence="18">
        <location>
            <begin position="21"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="DNA/RNA non-specific endonuclease/pyrophosphatase/phosphodiesterase" evidence="20">
        <location>
            <begin position="91"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="ENPP1-3/EXOG-like endonuclease/phosphodiesterase" evidence="19">
        <location>
            <begin position="92"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="156"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR640255-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR640255-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU366055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="CAZ85367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="338" mass="36561" checksum="1C3A3ABCB76B576D" modified="2010-06-15" version="1">MSKATLGLIAAASAAAGSAATAIYYSTVNNQKIVPPAVPATKGIPATSGGRLPPLVGITSSPVKAAQSRKPVDPSGLFQYGFPGPVNDLATRAALVSAFDRRTRNPIWVAEHITPESVAAKEGDRHRSKFVEDHDVPEKFRARLNDYRGSGYDRGHQVPAADAKFSQEAMDDTFFLTNMCPQVGAGFNRDYWSHFEDFCRRLTSRYPSVRIVTGPLYLPRRDSGGKYHVSYEVIGNPPNVAVPTHFYKVILAEDGVVGGTVAVGAFVLPNAVIDNSTRLEEFSVPLSIVERASGVEFLSKLPVNRRKELCKEIECSITVLEFNNASKSVTGVPAPGKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLH6</accession>
    <name>D5GLH6_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ribosomal RNA-processing protein 43</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00010208001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase PH family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430349">
        <property type="protein sequence ID" value="CAZ85369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841178.1">
        <property type="nucleotide sequence ID" value="XM_002841132.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLH6"/>
    <dbReference type="FunCoup" id="D5GLH6">
        <property type="interactions" value="209"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GLH6"/>
    <dbReference type="GeneID" id="9186113"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010208001"/>
    <dbReference type="eggNOG" id="KOG1613">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038194_5_0_1"/>
    <dbReference type="InParanoid" id="D5GLH6"/>
    <dbReference type="OMA" id="EIKAFWV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035925">
        <property type="term" value="F:mRNA 3'-UTR AU-rich region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071028">
        <property type="term" value="P:nuclear mRNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034473">
        <property type="term" value="P:U1 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034476">
        <property type="term" value="P:U5 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050590">
        <property type="entry name" value="Exosome_comp_Rrp42_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097:SF9">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097">
        <property type="entry name" value="EXOSOME COMPLEX EXONUCLEASE RIBOSOMAL RNA PROCESSING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="Ribonuclease PH domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0271">Exosome</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="11">
        <location>
            <begin position="51"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="37"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="50"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ85369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="330" mass="35384" checksum="9E433BC50D0D692B" modified="2010-06-15" version="1">MTTAPPPSPPPPNPLSFPPATFQKLAPKIFLERHLQSTPATRPSGRNPDTFRTPSLHTNSLSHAHGSAVVRTGDTAVVCGVRGEVLSLAAGTGGRIVVLRTGEDGRGKNGEGEGDEVRERGLVVTNLEMGTGCSRDYHPGPPTDFAQAVTARLHRLLLSNKLISSQSLRILDPNTEEVKAYWVLYIDAVVISLDGNVLDAAWASILAALRSTRLPQAYWDQDTEVVVCYPEVSRYKSLDVKGLAFTASFAVIDLGAGKGGKVILSDPDEFEEGVCGERVMVCALEGEKVGWIEKGGGVGQVAEVVECVHRATERFKIWEQLFAEVLGSSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLU2</accession>
    <name>D5GLU2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">mRNA stability protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00010420001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Plays an essential role in initiation of the G0 program by preventing the degradation of specific nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the endosulfine family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430351">
        <property type="protein sequence ID" value="CAZ85509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841318.1">
        <property type="nucleotide sequence ID" value="XM_002841272.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLU2"/>
    <dbReference type="FunCoup" id="D5GLU2">
        <property type="interactions" value="48"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GLU2"/>
    <dbReference type="GeneID" id="9186493"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010420001"/>
    <dbReference type="eggNOG" id="KOG4076">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101493_1_0_1"/>
    <dbReference type="InParanoid" id="D5GLU2"/>
    <dbReference type="OMA" id="TNSNMTQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004865">
        <property type="term" value="F:protein serine/threonine phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905287">
        <property type="term" value="P:positive regulation of G2/M transition of mitotic cell cycle involved in cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900237">
        <property type="term" value="P:positive regulation of induction of conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902472">
        <property type="term" value="P:regulation of mitotic cytokinesis, division site positioning"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006760">
        <property type="entry name" value="Endosulphine"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10358">
        <property type="entry name" value="ENDOSULFINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10358:SF6">
        <property type="entry name" value="ENDOSULFINE, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04667">
        <property type="entry name" value="Endosulfine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="56"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="115"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ85509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="147" mass="15432" checksum="64E484F6E1BC2220" modified="2010-06-15" version="1">MNPHQKQKVDISSLNEEEQRLFRLYGKLPSKKDILKNKLKERKYFDSGDYALSKAGKASDVGVTNIGSQHPLPENIPHHTAAGTPTGASGNSGSPVKESPLLHAKSPMGSQPPTEGEDVGDAEEASAVASEKTVGGSAAPAVPIPSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLW5</accession>
    <name>D5GLW5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Dolichol phosphate-mannose biosynthesis regulatory protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00010446001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Regulatory subunit of the dolichol-phosphate mannose (DPM) synthase complex; essential for the ER localization.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the dolichol-phosphate mannose (DPM) synthase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Endoplasmic reticulum membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the DPM2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="FN430351">
        <property type="protein sequence ID" value="CAZ85532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841341.1">
        <property type="nucleotide sequence ID" value="XM_002841295.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLW5"/>
    <dbReference type="FunCoup" id="D5GLW5">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GLW5"/>
    <dbReference type="GeneID" id="9187312"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010446001"/>
    <dbReference type="eggNOG" id="KOG3488">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_150144_2_1_1"/>
    <dbReference type="InParanoid" id="D5GLW5"/>
    <dbReference type="OMA" id="YTLWIIV"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033185">
        <property type="term" value="C:dolichol-phosphate-mannose synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0180047">
        <property type="term" value="P:dolichol phosphate mannose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009914">
        <property type="entry name" value="DPM2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15039">
        <property type="entry name" value="DOLICHOL PHOSPHATE-MANNOSE BIOSYNTHESIS REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15039:SF11">
        <property type="entry name" value="DOLICHOL PHOSPHATE-MANNOSE BIOSYNTHESIS REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07297">
        <property type="entry name" value="DPM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ85532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="83" mass="9327" checksum="E53B20F4F2AFB86A" modified="2010-06-15" version="1">MLMVASIVFLYYSIWTLFMPFVDEGHQLHDLFPPRVWAIRIPVILILVGIAVVGSFLSVVMIRSGRKKAAKARAKASQGKKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GM36</accession>
    <name>D5GM36_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00010509001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430351">
        <property type="protein sequence ID" value="CAZ85579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GM36"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010509001"/>
    <dbReference type="HOGENOM" id="CLU_1778814_0_0_1"/>
    <dbReference type="InParanoid" id="D5GM36"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ85579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="146" mass="15968" checksum="ABC1CB2DF5EAFC3C" modified="2010-06-15" version="1">MALLTGLRARVAKIRPTGASSDRVLARGVFSVFHDCADSSSGRFTCRDESLSIRVSLQSRSTVIILFFFSFSLPTEVRMVGYGLMFVKGVTHHTSGPEKRRGVESSGGNFRVGYHEVDTRAGGSWNTGRGLSRAWLSRLVLQVSVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMX1</accession>
    <name>D5GMX1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00010978001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430360">
        <property type="protein sequence ID" value="CAZ85864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841673.1">
        <property type="nucleotide sequence ID" value="XM_002841627.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMX1"/>
    <dbReference type="STRING" id="656061.D5GMX1"/>
    <dbReference type="GeneID" id="9183432"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010978001"/>
    <dbReference type="eggNOG" id="KOG1116">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021934_1_0_1"/>
    <dbReference type="InParanoid" id="D5GMX1"/>
    <dbReference type="OMA" id="YGFHASI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001727">
        <property type="term" value="F:lipid kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046512">
        <property type="term" value="P:sphingosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001206">
        <property type="entry name" value="Diacylglycerol_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050187">
        <property type="entry name" value="Lipid_Phosphate_FormReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12358:SF108">
        <property type="entry name" value="DAGKC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12358">
        <property type="entry name" value="SPHINGOSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00781">
        <property type="entry name" value="DAGK_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50146">
        <property type="entry name" value="DAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DAGKc" evidence="1">
        <location>
            <begin position="89"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="428" mass="46156" checksum="4768634F514C97FC" modified="2010-06-15" version="1">MELPTDSTPTPAYISDSECGERLTFCQDEEKALSIPLEYLLALVPAETSNAYTLISQTGPGGGVISSTITSPPAEIIERYCIPVDTPAHLQKPVSVVTSPYSGLGRAELYFSTLLAPVLEYYSVDYGKHTVTSPTAIKTIANGLPEGTSTVIILSGDTLIFELLNQLPSTKRPTLVLIPTGTGNGLASSLYRPSPPDDLIRSAFHTFLHGISHPLPGFAVKFSPGARLLEEAIPEQGLKGTVVTSWGFHASLVADASTPEMRSSGTEKFQVAAKQNLTPSLHAYRGTISIVRPGSTKWEEVGDTNDEGHFYLVVTGVSNFEPIFTISPAANGMDGILRIIYFGVGDVEGAEGVMGVMKAAYDGGKHVSIPGVRYQEVDGVKVHLKEAEEKWRRICVDGAVVTLEEEGWLEVNRHEGWADVVYRSRFDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5GN14</accession>
    <name>D5GN14_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ribosomal protein L37</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00011013001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the eukaryotic ribosomal protein eL37 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430361">
        <property type="protein sequence ID" value="CAZ85889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841698.1">
        <property type="nucleotide sequence ID" value="XM_002841652.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GN14"/>
    <dbReference type="FunCoup" id="D5GN14">
        <property type="interactions" value="484"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GN14"/>
    <dbReference type="GeneID" id="9183457"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011013001"/>
    <dbReference type="eggNOG" id="KOG3475">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_150908_0_0_1"/>
    <dbReference type="InParanoid" id="D5GN14"/>
    <dbReference type="OMA" id="RMAYLKH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030684">
        <property type="term" value="C:preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.30:FF:000001">
        <property type="entry name" value="Ribosomal protein L37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00547">
        <property type="entry name" value="Ribosomal_eL37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001569">
        <property type="entry name" value="Ribosomal_eL37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011331">
        <property type="entry name" value="Ribosomal_eL37/eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018267">
        <property type="entry name" value="Ribosomal_eL37_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003214">
        <property type="entry name" value="PRK04179.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10768">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10768:SF0">
        <property type="entry name" value="RIBOSOMAL PROTEIN L37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01907">
        <property type="entry name" value="Ribosomal_L37e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01077">
        <property type="entry name" value="RIBOSOMAL_L37E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7 9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 9" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="5 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="76"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ85889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="97" mass="11063" checksum="0FE55A3515CF9332" modified="2010-06-15" version="1">MTKGTSSFGKRHNKSHTLCRRCGRRSFHIQKHTCASCGYPAAKVRKYNWSEKAKRRKTTGTGRMRYLKTVSRRFSNRFQLGAPKGARGPQSNADTVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GN17</accession>
    <name>D5GN17_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00011016001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430361">
        <property type="protein sequence ID" value="CAZ85892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841701.1">
        <property type="nucleotide sequence ID" value="XM_002841655.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GN17"/>
    <dbReference type="FunCoup" id="D5GN17">
        <property type="interactions" value="823"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GN17"/>
    <dbReference type="GeneID" id="9183460"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011016001"/>
    <dbReference type="eggNOG" id="KOG4210">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027451_1_1_1"/>
    <dbReference type="InParanoid" id="D5GN17"/>
    <dbReference type="OMA" id="RVWVNQL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF95">
        <property type="entry name" value="NUCLEOLAR PROTEIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="103"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="209"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="319"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="10"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="60"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="322"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ85892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="405" mass="44854" checksum="B41D87D7B0DBDBAB" modified="2010-06-15" version="1">MRNENLSSMAEEPTTQETAEADTSTPSKKRKRDTAVEDIEIDINAPEPPSKKALRKLKKSGATSISTGSSASTSALTAPITAPATAPKSQNFPEHASQSRSKWGVWIGNLAFSVTKPDLETFFTKPPSTIPRVGITRINLPTNPQTRRNKGFAYVDFSNQESLNYALILTETLWNGRPVLIKNAKDFSSRTPTSAIKVGADLSKNPPSRILWVGNLDFITKEEDLAKHFAFAGKIVKVRLTTFEDTGKCKGFGFVDFEDEESVKRAMLGLSPEDKTNPEIAEGKEEEELNRLRKKRGFIGGRKVKMEYGEDPSVRYKKRFGKEKDAQDNEDVTKGAPIALMGSRGRLTATGGVREQENDTKPIRTKKKSKFDNPKRSYNPGTAYSNDSRRTGAIIEGKGKRVKFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5GN95</accession>
    <name>D5GN95_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">D-serine dehydratase</fullName>
            <ecNumber evidence="11">4.3.1.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">D-serine deaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">GSTUM_00011166001</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the conversion of D-serine to pyruvate and ammonia. May play a role in D-serine detoxification.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>D-serine = pyruvate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:13977"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:35247"/>
            <dbReference type="EC" id="4.3.1.18"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:13978"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DSD1 family.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.18" evidence="11"/>
    <dbReference type="EMBL" id="FN430362">
        <property type="protein sequence ID" value="CAZ85988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841797.1">
        <property type="nucleotide sequence ID" value="XM_002841751.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GN95"/>
    <dbReference type="FunCoup" id="D5GN95">
        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GN95"/>
    <dbReference type="GeneID" id="9185914"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011166001"/>
    <dbReference type="eggNOG" id="ENOG502QWI8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031639_0_0_1"/>
    <dbReference type="InParanoid" id="D5GN95"/>
    <dbReference type="OMA" id="WPRFYGW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008721">
        <property type="term" value="F:D-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036088">
        <property type="term" value="P:D-serine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009636">
        <property type="term" value="P:response to toxic substance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.10:FF:000016">
        <property type="entry name" value="D-serine dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="entry name" value="Alanine racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.37.20">
        <property type="entry name" value="D-serine dehydratase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001608">
        <property type="entry name" value="Ala_racemase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051466">
        <property type="entry name" value="D-amino_acid_metab_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026956">
        <property type="entry name" value="D-ser_dehydrat-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042208">
        <property type="entry name" value="D-ser_dehydrat-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28004:SF2">
        <property type="entry name" value="D-SERINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28004">
        <property type="entry name" value="ZGC:162816-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01168">
        <property type="entry name" value="Ala_racemase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14031">
        <property type="entry name" value="D-ser_dehydrat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01119">
        <property type="entry name" value="D-ser_dehydrat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="PLP-binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0216">Detoxification</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="D-serine dehydratase-like" evidence="14">
        <location>
            <begin position="299"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00051198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00055764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00066349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00069616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00075219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAZ85988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="429" mass="46243" checksum="3FC861678BD05E5E" modified="2010-06-15" version="1">MAEYIGQKIHMLPTPSAVISRPVLEKNCSRVLQAVDALSVGFRPHVKTHKTVEVSRLQLGREARGGKVVISTVREAEGLGPLIMEGVVKDILYGMPIPVSSIYRLQRLEESYSGLEISVLVDHPAQVRALRAAGVDWNVYVKLDMGTRRAGIAHDSPAFGELLACVKESDIGLAGFYCHAGHSYSVSDSDEALALLREEVDTAVDAAGKAEAQGVLPPLFTGWTLSVGATPTIISTAGAGIPGQVPPKLQKMISFFADLREKGMHVEFHAGVYTMLDLQQLSTHASTATPEGLGTSDLALTILAEVVSLYPRRGASGENEALINVGTVGLGREPGRAYSGWGTVSSWRAENEAEFEHELEGAGWVVGRISQEHGILTEVPNGNVSSVRLGSKVRIWPQHACIAGSGYERYFVVDEDEVVVGVWARWRGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNN2</accession>
    <name>D5GNN2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Exocyst complex component SEC5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00011384001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the exocyst complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the SEC5 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430366">
        <property type="protein sequence ID" value="CAZ86125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841934.1">
        <property type="nucleotide sequence ID" value="XM_002841888.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNN2"/>
    <dbReference type="FunCoup" id="D5GNN2">
        <property type="interactions" value="313"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GNN2"/>
    <dbReference type="GeneID" id="9186201"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011384001"/>
    <dbReference type="eggNOG" id="KOG2347">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011308_0_0_1"/>
    <dbReference type="InParanoid" id="D5GNN2"/>
    <dbReference type="OMA" id="RMWMDVD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029175">
        <property type="entry name" value="EXOC2/Sec5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039481">
        <property type="entry name" value="EXOC2/Sec5_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13043:SF1">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13043">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT SEC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15469">
        <property type="entry name" value="Sec5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component EXOC2/Sec5 N-terminal" evidence="6">
        <location>
            <begin position="77"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="514"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU365069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF15469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ86125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="968" mass="106904" checksum="C46DE31EC2F5B901" modified="2010-06-15" version="1">MGDPRAEAALLKHYRLQNPFPETWSDPIEEAQIIQRQQSRASTSRYSILQEEGVSASLRGLVGGDVYSTGVIPEDEPDPLGTTGSVVRVLGKKGLPVRDNVRLRNRYLVSSKTFSPTAFLRDVHANTPSPALQQGLNYLSTSIAQKSGSLKLLVESNFDRFVAAKGTIEGVYKEMKGDSFLDKDRESEWGVGKIRGYLNDAGAKADEVFGPAMRGRGREEGLRLALAVLEKHRAMLEMPGNLSECIKRKDYESLIEEYQKARSFLEGSRKLVPTGNMAPAKEDHIHQLVIAENMWAEVTIVIKDFKRDTWKRLAECPTEGNLHMELIGILLELGVDDNPVWLWLLSRYDYLKNRITTLFERSRVEIEVLRRKIVEAPPPTPKSVAHHLRSPSRRAHVDSSKSLDTHLITSFWELVYSSLSALLSPLSGLVGELLSFQKTAQSFIDGDNLLPVGIDDQSRKHHRLEPDDARKLQEGIYELALMIRDCLVKFYTEPPVEDISAMYSPIPETPLSANTPGQDPNFPPPGPAVPGAQAQKDEDEAYAFVPPGTNSLSGAHYMSKILVLTGNASVAICEMVSRLGRADFAERFRAMVSGSRERAVVAVCAAWLKDAGNCKFLEDWTRAGDNRDVTRMPGLFGAFEREVISGMQAIVYLDKVVRSDSSVIPPPSAKLLSHVREQFVRSLYKSLQGMVENAKTPLDLDARDGDQTDLASPVTSVAPRNLTAYSVDSKDKNVRILLTLSNLQLLRNDVVPELIGVFENAFSVKLTDESKTIRDAQSQIDAQLFEEYTKPLVSSLSDTIRLGVLSPSWPPPPGKLATSVRPYIYTALLSLVLVHSQITTTAPSLTHPILSYLLEAISGELLEAFKQRHRFSLGELLQAALDVEFVNQTLSQYNTPRASELQQGVYVELDRGSDAEARLGLREGLGGMKKILGELRGGSRAEFLCFRAKKSSSKKTPAAVAGAATAEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNT8</accession>
    <name>D5GNT8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00011477001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430368">
        <property type="protein sequence ID" value="CAZ86181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841990.1">
        <property type="nucleotide sequence ID" value="XM_002841944.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNT8"/>
    <dbReference type="STRING" id="656061.D5GNT8"/>
    <dbReference type="GeneID" id="9182816"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011477001"/>
    <dbReference type="eggNOG" id="KOG3346">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_592107_0_0_1"/>
    <dbReference type="InParanoid" id="D5GNT8"/>
    <dbReference type="OMA" id="CTSKIAP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00920">
        <property type="entry name" value="Cupredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00866">
        <property type="entry name" value="PEBP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.280.10">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008914">
        <property type="entry name" value="PEBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036610">
        <property type="entry name" value="PEBP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035810">
        <property type="entry name" value="PEBP_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052953">
        <property type="entry name" value="Ser-rich/MCO-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34883:SF4">
        <property type="entry name" value="CUPREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34883">
        <property type="entry name" value="SERINE-RICH PROTEIN, PUTATIVE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01161">
        <property type="entry name" value="PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49777">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072491" evidence="2">
        <location>
            <begin position="24"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="226"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ86181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="462" mass="47967" checksum="7FBEE1F5F0DF9C7E" modified="2010-06-15" version="1">MFGKRTGFIAAVAAVLFSGSVLAQIPDAFSAGFDPKSIQLEVTFGSKELKDGDVLTPADAAALPKFALGGSSGISPKTKYCILMIDPDSPNRDDAVAPQVLHYLKTDFTVFELTNLASQSNPTLKYVGPDASTGSGPHRYIFLLYVQPDGFSVQGVPGESNRGGFNVSSWREVNNLKRAVAGIHFVATPPAGGAAPSSAAGAESKTAVAPPASTLTSNGVVYTTTPPPCTTSTSKSAQSVPVQTTSQPVAVPSKSQPPMTYTSVPPAQTSVPAGPPKTHTVKVGGPNLLKYTPENVIANVGDVVYFDFLERNHTVTQSTFDNPCVLNPNGVRSGFRPNPQNIPGKETFNVTVSDDKPKWFYCAQGTHCKAGMVFAINPGAKFEEFVNKAKSGGNATTTAGGPAVTGTPNPSAPVTLIPSGTITPPVNSTAPTGTPVFTGAGSRVTFSITTFVGVFLTAWLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>D5GP60</accession>
    <name>D5GP60_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00011720001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430373">
        <property type="protein sequence ID" value="CAZ86325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842134.1">
        <property type="nucleotide sequence ID" value="XM_002842088.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GP60"/>
    <dbReference type="GeneID" id="9186565"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011720001"/>
    <dbReference type="HOGENOM" id="CLU_1138715_0_0_1"/>
    <dbReference type="InParanoid" id="D5GP60"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ86325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="244" mass="26476" checksum="192E9682C426AD66" modified="2010-06-15" version="1">MAEPEKPTLFDSIRRYTNTQISGVLHSLIGVPSMLQPPTFSDMDNTPTSDTTSTLSQPTTPSGEGSQQVVEGRGDGKVERVISEEWKIIERQLFLRDPEEVFSSFLKDTGVGLKGSWAEEVFWPRGRFGDVWVRGVCRGGEADVKGKGGEEGERAKATGEGGEGRETELDAYAQLLPLSTRPENKTGGAVTGSRGQGSLGVVSSVATTRSVTENGVTKTEYILKRRFSDGQEEFLQKRITRGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPA3</accession>
    <name>D5GPA3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00011774001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430373">
        <property type="protein sequence ID" value="CAZ86368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842177.1">
        <property type="nucleotide sequence ID" value="XM_002842131.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPA3"/>
    <dbReference type="FunCoup" id="D5GPA3">
        <property type="interactions" value="480"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GPA3"/>
    <dbReference type="GeneID" id="9186608"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011774001"/>
    <dbReference type="eggNOG" id="KOG1690">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066963_2_1_1"/>
    <dbReference type="InParanoid" id="D5GPA3"/>
    <dbReference type="OMA" id="GATCAWQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="GOLD" evidence="8">
        <location>
            <begin position="33"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ86368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="223" mass="25272" checksum="B7A37D95637243B1" modified="2010-06-15" version="1">MLPSIASPLTLLLAASYALPSFGLYFYIDGITPKCFFEELPKDTLVVGQYTAEEYNPNIQAYHNNPQLAIQITVDETFDNDHRVVNQKGSSSGRFTFTAGDSGEHRLCFWASHASSGGWFSNHPEGVKLTLDLAIGETSEIESKDKDKLADIVQRVKDLNSRLQDIRREQVFQREREAEFRDQSESTNARVVRWTLIQLALLGVTCAWQLSHLRAFFIKQKLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPJ4</accession>
    <name>D5GPJ4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00011872001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430375">
        <property type="protein sequence ID" value="CAZ86437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPJ4"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011872001"/>
    <dbReference type="eggNOG" id="ENOG502S8NN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101051_0_0_1"/>
    <dbReference type="InParanoid" id="D5GPJ4"/>
    <dbReference type="OMA" id="FKAPKTT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053203">
        <property type="entry name" value="Cisplatin_resist-associated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022024">
        <property type="entry name" value="DUF3602"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34693:SF2">
        <property type="entry name" value="DUF3602 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34693">
        <property type="entry name" value="PROTEIN PAR32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12223">
        <property type="entry name" value="DUF3602"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="125"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ86437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="166" mass="17485" checksum="79F8EC8851B3025A" modified="2010-06-15" version="1">MPFQLTEGTPVHDAGSYYHAGRGGAGNYRRLPSTTSLPATVATALPAATTPSNPRVFFGGRGGAGNIKPKSERAMFSFDEELERDRLFHEHHAPVYSIGRGGAGNIVPSEAIATRSHYSVSPAGSPDSTPRSSTSSPSSSPRFSSHSLRSGADRVWGSIARVRSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPN6</accession>
    <name>D5GPN6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00011948001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430377">
        <property type="protein sequence ID" value="CAZ86479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842288.1">
        <property type="nucleotide sequence ID" value="XM_002842242.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPN6"/>
    <dbReference type="STRING" id="656061.D5GPN6"/>
    <dbReference type="GeneID" id="9186719"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011948001"/>
    <dbReference type="eggNOG" id="KOG2699">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_534160_0_0_1"/>
    <dbReference type="InParanoid" id="D5GPN6"/>
    <dbReference type="OMA" id="APKYDWG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012506">
        <property type="term" value="C:vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16105">
        <property type="entry name" value="Ubl_ASPSCR1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021569">
        <property type="entry name" value="TUG-UBL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46467">
        <property type="entry name" value="TETHER CONTAINING UBX DOMAIN FOR GLUT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46467:SF1">
        <property type="entry name" value="TETHER CONTAINING UBX DOMAIN FOR GLUT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11470">
        <property type="entry name" value="TUG-UBL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBX" evidence="2">
        <location>
            <begin position="349"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="264"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="444"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="219"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="456"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="480"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ86479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="501" mass="54590" checksum="EB1689A788AFF96B" modified="2010-06-15" version="1">MSLATVVVIYGGRRASIKAPPTRSLSEIHTEACIKFGVDPQNFALKKGNAALDLSLQIRLTNLSPGAKLDLVLAKNRDAPITIVLRAQEADGRPIQEFTDMFTASSTIWEIMRKLEAKGPRTNNDPAINITERAAPSGTSGSGRLHYVMPVARVMNREVSGFRELQQTLSDFGIRSGRQLVTLRYRETDIPFEAALDEIAGFSAPREKATEAEVQGGESSKDAEGEKRKAEAGFIMFDAPEDPDKQGDTGGSNAMEVDKPMEGSADALETEGGLPEPPNSDKPVVSVFRPSQSNTLAATQIDVPDKAYEVGITEAKLIQANIKKETIGKRLPSDREIEERKAAALAEAEKVERVVIKVRFPDGYTSEQNFDKTHTSQDLYDTVRGTLRHPNEPFLLRIPPRETLPQAPKRLTTDLKLRTGATVHLVWGPDVSAKAKKELALKDEFIENSKALPTPEEPEGPEGESDDNPGGGLSKGKGKSGGDRASKENKLMGILRFGKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPY3</accession>
    <name>D5GPY3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00012089001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430380">
        <property type="protein sequence ID" value="CAZ86576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842385.1">
        <property type="nucleotide sequence ID" value="XM_002842339.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPY3"/>
    <dbReference type="STRING" id="656061.D5GPY3"/>
    <dbReference type="GeneID" id="9182320"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012089001"/>
    <dbReference type="eggNOG" id="KOG1205">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_9_1"/>
    <dbReference type="InParanoid" id="D5GPY3"/>
    <dbReference type="OMA" id="VWEAYNI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05374">
        <property type="entry name" value="17beta-HSD-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051911">
        <property type="entry name" value="SDR_oxidoreductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43976">
        <property type="entry name" value="SHORT CHAIN DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43976:SF16">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE_REDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ86576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="290" mass="31871" checksum="B6B51939F6884FA6" modified="2010-06-15" version="1">MAAPRVWLVTGTSSGFGNELVHTILKNGHKVVAAGRNTSRLTSLKEAGAATTKIDQNEPLDILRKQVEDAITIYGHIDVIVLNAAYVTTGTLEERTPEESLAQYHTNVFGILNLYRAILPHFRVRKAGIIATVGSMGASMTDPLIGLYHSSKAAVRNLSLTLDNEVKPFGIKTCLIEPGYFRTNLLSSGTSFQLPETKIEAYAEINEKTKTFLTERDRKQSGDPKRGAQVIFDVLTSSGCARGREVPKTLALGSDAVKCIIGSNEEFNKRVEEWKSVSESTDFPKEEQHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>D5G3U5</accession>
    <name>D5G3U5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00003808001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835067.1">
        <property type="nucleotide sequence ID" value="XM_002835021.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G3U5"/>
    <dbReference type="STRING" id="656061.D5G3U5"/>
    <dbReference type="GeneID" id="9187126"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003808001"/>
    <dbReference type="eggNOG" id="KOG2651">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016581_1_1_1"/>
    <dbReference type="InParanoid" id="D5G3U5"/>
    <dbReference type="OMA" id="LQAPYNW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052220">
        <property type="entry name" value="METTL25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12496">
        <property type="entry name" value="CGI-41 METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12496:SF0">
        <property type="entry name" value="METHYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13679">
        <property type="entry name" value="Methyltransf_32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase" evidence="2">
        <location>
            <begin position="144"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="224"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="255"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ79188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="537" mass="59240" checksum="946450B72DBCDA2C" modified="2010-06-15" version="1">MPPTPLLPYSPQFTSPEEYITSLLTFTSNPLFQTLCGGVHILDFFTRDSGTKPVDLYHEILPADWISFFSVQEIPSVLDHLLRTPIDQLPKTCPETLVTFITQVRIHSLNRDFTRPPALAKAKTSAGVSHSGEEWALNAGMRPKKIHEVENFSAFIDDLVSNINARDKPIGGEGITHILDFGSGQAYLSRTLAKKYGHNVVGIESRPANIEGAKEMDTRFDSLAERRERKGRKSQQSREPTSRVPNDDPAAEETVPTTTNDGKSGTLQYIQKCISGSDLTPITTLIPSPTPKSLLLISLHSCGNLLHHGLISFATTPSVKACALIGCCYNLLTEKSGATYKPPLRSPHPRLAATSAAADPDGFPLSHRFTHTNPAVRLNITARMMACQAPSNWTPSSSEAFFTRHFFRALLQRIFFDRGLVTGAEPVIIGSLRKRCYSSFGDYVSGAIDKIGWAQRLQVSAGEVAQYEERFTCRKKDISVIWSLMAFCAGVAESLIVVDRWVYLMEGVEKGLVKEAWVEAGFEYGISPRNLVVVGVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>D5G477</accession>
    <name>D5G477_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00003984001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835199.1">
        <property type="nucleotide sequence ID" value="XM_002835153.1"/>
    </dbReference>
    <dbReference type="GeneID" id="9184296"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003984001"/>
    <dbReference type="eggNOG" id="ENOG502SSBZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_418082_0_0_1"/>
    <dbReference type="InParanoid" id="D5G477"/>
    <dbReference type="OMA" id="VSHPRAY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058348">
        <property type="entry name" value="DUF8035"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26118">
        <property type="entry name" value="DUF8035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF8035" evidence="2">
        <location>
            <begin position="529"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="494"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="627"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="249"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="321"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="511"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ79320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="656" mass="70526" checksum="551EA0B144D31F3C" modified="2010-06-15" version="1">MSHRLQSPNRNRKKRRDDPHRISTGSLQGGQDGYYGGAPPAAPPAPAPPYYNTHSVPQSPISSNESIGTFPLGSRQGHMHQHPSHHGYPPAPPPPGMRGGDAGHMYNGGRTHVGRNAQARNRQNVIHQQPSQNMPEYEPHPEFDFRPLPPRVPPAAHRESRSSSRTRDGDLSPPGSAGENSNFSGASFDHVDPHKRFVVPSSRFSGNREYRLNGPLPGERDYRGGGGTIYSGGSRAVLGGERRRERSMSRGPHGLGLSSVSGDGLSDEYFEEQHAYDPRSGGRRMVSSGAVDPNAGALVRSRSRSAPPESRAMISQHAPASESDGSSSSSSSGTAPQPGTGIRLEYAGNSVDITYTGSGGKPVSIGSITINTTARTTAIPISPTTQAIAAPPMQAIAAPPSAPAPAALPAPPPPPVQPVMIPPPPAPVAVPPPGIEPLILPPPPAPMMPGSMPGPTPASMPGRDMGIYGDDERGMRDHMFRSMEALALEPRYDDRGRGREVMPPGGMTALSHRRRSQSRGRKPADDELRWTKISRDIVARRAVEVKGYDFEEQPDSIVVFQVLDEKQIDELFDLSERIRSGEVRVIRRDDSGKEPKRRRRRHSXXXXXXXXXXXXXXXXXXXXXXXXXTCPTPPAICGRKAHGGSEQAGDVHGIQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4F8</accession>
    <name>D5G4F8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Glutathione S-transferase omega-like 2</fullName>
            <ecNumber evidence="3">1.8.5.1</ecNumber>
            <ecNumber evidence="4">2.5.1.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Glutathione-dependent dehydroascorbate reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">GSTUM_00004090001</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Active as '1-Cys' thiol transferase against beta-hydroxyethyl disulfide (HED), as dehydroascorbate reductase and as dimethylarsinic acid reductase, while not active against the standard GST substrate 1-chloro-2,4-dinitrobenzene (CDNB). May be involved in cell wall organization and biogenesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-dehydroascorbate + 2 glutathione = glutathione disulfide + L-ascorbate</text>
            <dbReference type="Rhea" id="RHEA:24424"/>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58297"/>
            <dbReference type="ChEBI" id="CHEBI:58539"/>
            <dbReference type="EC" id="1.8.5.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GST superfamily. Omega family.</text>
    </comment>
    <dbReference type="EC" id="1.8.5.1" evidence="3"/>
    <dbReference type="EC" id="2.5.1.18" evidence="4"/>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835280.1">
        <property type="nucleotide sequence ID" value="XM_002835234.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4F8"/>
    <dbReference type="FunCoup" id="D5G4F8">
        <property type="interactions" value="149"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G4F8"/>
    <dbReference type="GeneID" id="9185026"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004090001"/>
    <dbReference type="eggNOG" id="KOG2903">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037263_0_1_1"/>
    <dbReference type="InParanoid" id="D5G4F8"/>
    <dbReference type="OMA" id="PWANRAI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045174">
        <property type="term" value="F:glutathione dehydrogenase (ascorbate) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03190">
        <property type="entry name" value="GST_C_Omega_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000162">
        <property type="entry name" value="Glutathione S-transferase Gst3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000038">
        <property type="entry name" value="Glutathione S-transferase omega-like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047047">
        <property type="entry name" value="GST_Omega-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016639">
        <property type="entry name" value="GST_Omega/GSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32419:SF6">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE OMEGA-LIKE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32419">
        <property type="entry name" value="GLUTATHIONYL-HYDROQUINONE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13410">
        <property type="entry name" value="GST_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13409">
        <property type="entry name" value="GST_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015753">
        <property type="entry name" value="GST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01206">
        <property type="entry name" value="Xi.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01148">
        <property type="entry name" value="Xi_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST C-terminal" evidence="17">
        <location>
            <begin position="206"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="14">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="14">
        <location>
            <position position="229"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>glutathione</name>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="161"/>
            <end position="164"/>
        </location>
        <ligand>
            <name>glutathione</name>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="179"/>
            <end position="180"/>
        </location>
        <ligand>
            <name>glutathione</name>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Cys" evidence="16">
        <location>
            <position position="289"/>
        </location>
    </feature>
    <feature type="site" description="Lowers pKa of active site Cys" evidence="16">
        <location>
            <position position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00055859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00070045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR015753-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR015753-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR015753-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAZ79401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="360" mass="41820" checksum="4DADE6B729934757" modified="2010-06-15" version="1">MFFRSIFRQTFSHLSSPLKASPSLRYQLNYRAMSTSGQKITDWCTPDSKRGEFNRKPSTFCDLVSREPNAKFPPERGRYHLYVSYACPWAHRTLITRELKGLQDIIGVSVVHWHLGENGWRFGTTEEAGEYPEVAVGPEANTGASFLRDIYFKAQPDYDGRYTVPTFWDKKNNTIVSNESSEIIRFLYTEFDDLVDESKKGITYYPEDLRKEIDELNEWIYATVNNGVYKAGFATKQEAYEVAVTKLFESLDRIEKILKDSDGPYLLGKVLTEADIRLYPTIARFDPVYVQHFKCNLRMIRHDYPAIEKWMKHLYYDIPGFKETTNFEHIKKHYTKSHSQINPKGITPLGPAPDIMPRDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>D5G541</accession>
    <name>D5G541_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="2 7">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00000242001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the peptidase C12 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="2 7"/>
    <dbReference type="EMBL" id="FN429996">
        <property type="protein sequence ID" value="CAZ79626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835469.1">
        <property type="nucleotide sequence ID" value="XM_002835423.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G541"/>
    <dbReference type="STRING" id="656061.D5G541"/>
    <dbReference type="GeneID" id="9186324"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000242001"/>
    <dbReference type="eggNOG" id="KOG2778">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018316_3_1_1"/>
    <dbReference type="InParanoid" id="D5G541"/>
    <dbReference type="OMA" id="CLWELDG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.532.10">
        <property type="entry name" value="Peptidase C12, ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001578">
        <property type="entry name" value="Peptidase_C12_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036959">
        <property type="entry name" value="Peptidase_C12_UCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10589">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10589:SF29">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01088">
        <property type="entry name" value="Peptidase_C12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52048">
        <property type="entry name" value="UCH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UCH catalytic" evidence="9">
        <location>
            <begin position="37"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="194"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="118"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="229"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="7">
        <location>
            <position position="112"/>
        </location>
    </feature>
    <feature type="site" description="Important for enzyme activity" evidence="7">
        <location>
            <position position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS52048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ79626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="425" mass="48041" checksum="FE891E63E70E4F19" modified="2010-06-15" version="1">MASSSNIGGSTRVKSNISPCAAPEIRGIVEQKENWGGWATTLNDATIFEYIMRQVGCESVAVRELYSMEEDYLREVKSCYGIIFLMPYNEHYETQAGEELKDLPDGMWYSNQIADNACASYALLNILMNLNNVPLGTQLEGFREFTLGLLPPHRGQAVDGFKSIKNAHNSFCFRYEIEEADLILYEEAVRDSDPKSELKSKENHGGKKYKTEEAEVARGHTDQIDGPHHYIAYVQVGGCLWELDGLKQTPFKVGDCNNENFAVCVGSRMRELTARCSDLSIDFGIFALEPAKAPQLDKQHQANAREIGAIERKLQEIDPHSSLRKEDLLSGLLILPPSDTDDFGHHNGMMTEQLVELRTDLIQKQKAIEMEKDNLFNEESRKALELARNRHDYEPFILTMATMVWAQQQAEAKTIKPKKKGRKGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>D5G557</accession>
    <name>D5G557_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00000264001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835485.1">
        <property type="nucleotide sequence ID" value="XM_002835439.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G557"/>
    <dbReference type="GeneID" id="9186340"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000264001"/>
    <dbReference type="eggNOG" id="ENOG502QWQE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033097_1_0_1"/>
    <dbReference type="InParanoid" id="D5G557"/>
    <dbReference type="OMA" id="WLPLWFY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018830">
        <property type="entry name" value="DUF2434"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10361">
        <property type="entry name" value="DUF2434"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="223"/>
        </location>
    </feature>
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        <location>
            <begin position="243"/>
            <end position="263"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="275"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="308"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="375" mass="43004" checksum="754FDC27BA29478B" modified="2010-06-15" version="1">MAKPFNSSHIQFGGQVFNETLLQEFNYGIYSNGTLSNSSRCYLVFDTYRPWIASNGSVFNGTKCDSPINPIATRGALGITFAVLYAVVIVFVCVHLRKHGATHLPLEKRFRLVSRRWQWYWLMITCACGMMSGFTAIDIDRDYLMGTPFILSVLFYWVMVPTTLAAVWETTRHWGSFLERQIMESSPFKLSQEDRRSKIEFYVPLVFYLFAFLTFFLSILRSWNPIRKGFKSAITDPRFRTSAVFSLIALLVIVFSFCVSIHYYKPLSHRIPAKIPICITLAFVRVAYAIACSWEADINPLSIHASPAYVYALGYLPSLGIMLTMIVAGYREMNEDLFIKKLKAERIRLADGEIEKGRREELKKKEEEGLKKVGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5G589</accession>
    <name>D5G589_TUBMM</name>
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        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
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    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
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            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
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            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="KEGG" id="tml:GSTUM_00004220001"/>
    <dbReference type="HOGENOM" id="CLU_2401274_0_0_1"/>
    <dbReference type="InParanoid" id="D5G589"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="93" mass="10062" checksum="B57CACB4A1223DCD" modified="2010-06-15" version="1">MNIKPRLSSIPIFRTPNPGRPTPPTLSTPSLAWNTGTKSSRTQHPAVQQRKSQKIGAQRPFGTSPGGMARGLSRTRSVAFPTHQGGWDPSDVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5K6</accession>
    <name>D5G5K6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00004385001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
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            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835642.1">
        <property type="nucleotide sequence ID" value="XM_002835596.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5K6"/>
    <dbReference type="STRING" id="656061.D5G5K6"/>
    <dbReference type="GeneID" id="9183785"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004385001"/>
    <dbReference type="eggNOG" id="ENOG502S0TP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023193_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5K6"/>
    <dbReference type="OMA" id="NKPTGFE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033167">
        <property type="term" value="C:ARC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018606">
        <property type="entry name" value="Arb1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09692">
        <property type="entry name" value="Arb1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="336"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <begin position="336"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="349"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="457" mass="51447" checksum="82E7689F05E650DE" modified="2010-06-15" version="1">MSESAEASLSEAIGKLEIVNAGEEYEDEDVPKLEGVRIGELEDQQAPKKKKKRKKNKPKNPRPSGFEEHHVEGPLTLDAVLEEQSLYHPTKPFSERIEICIQRYKAKRKFDTLRSQIFTTYLTLGGISTGPKQFSGGLDYRQNDHLEKEQLEAMAATDFVSTNLDEDDEWEVDFPFVVRGFLSHRVPYVLGYIEVAHLELAAQVVRNFLNYVLYHNVAPEHADGIKEAIQICDLAEKELALCRHTSHRLPGNFNMACSTLFGGHYAGMKDETQTWDTLTPPVGLTTREATDIFNACVQARGTPEQKEMGLDVKITKAEFMGLEVIGIVFPNLERKRGKDEAKADEPEGSETNTDENTNPAAKPYEATGLKALGRLLVKPWTPDDELHPETDLIEFEIWLELEVLQFCFSGMHLEAKVHKLNSGIFWIDSVTAVQCSFYLQLDPPDKAGDNASDDDFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5R1</accession>
    <name>D5G5R1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00001444001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430001">
        <property type="protein sequence ID" value="CAZ79854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835697.1">
        <property type="nucleotide sequence ID" value="XM_002835651.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5R1"/>
    <dbReference type="STRING" id="656061.D5G5R1"/>
    <dbReference type="GeneID" id="9187532"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001444001"/>
    <dbReference type="HOGENOM" id="CLU_1620267_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5R1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="164" mass="16966" checksum="221F32BBA18AFF5C" modified="2010-06-15" version="1">MAGKNLKEGDEGHVARDGNDVVKRASEPTKESTDIANDSATSSSAAAAAEPSNANGEGKKEVELGEKRDRVEDEEKPAAQELAEAEKDAGKETKKPKLAGDAADGEGENSGARNGADGAASPPKKRGPGRPKKSEREAKKRDEAAKPEASSAETISGRTRSKAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5Y0</accession>
    <name>D5G5Y0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00001620001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430002">
        <property type="protein sequence ID" value="CAZ79923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835766.1">
        <property type="nucleotide sequence ID" value="XM_002835720.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5Y0"/>
    <dbReference type="STRING" id="656061.D5G5Y0"/>
    <dbReference type="GeneID" id="9187601"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001620001"/>
    <dbReference type="eggNOG" id="ENOG502QSSH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036627_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5Y0"/>
    <dbReference type="OMA" id="NHWNAWK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025337">
        <property type="entry name" value="Questin_oxidase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35870:SF6">
        <property type="entry name" value="MGS207 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35870">
        <property type="entry name" value="PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G03330)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14027">
        <property type="entry name" value="Questin_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ79923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="423" mass="48248" checksum="D449541EE5B799EC" modified="2010-06-15" version="1">MGNAKSTPLNNPYTGLPRVPTTNPSASTDKRDRTLTHLLRLNHHNYSLLYSQLRFHNHLPHALGSAYLLDAPSEHLTRLYESESKPLEPWPPTTPGEIARHDWREHISDRHYQRSYLDFFEDELVRLGYDWRRVVAEYLFQGPEPLIFGGIGGLGHPLIHLGYAFELGSKEVGMEALSLFATNYDFLHGYVDSEFPTAAGGVPVESPSPPLPTVVHDCQDPLEIIHRIRMDTAFHGIFPHAGAENIPLLFELREKEVLAYYRQFKIESPATAHKSLNHLAALLLTCSHDPGRPEFDFFVVHLTTVSYAIRILLPEVPEEYARPLVKMQWLFTIVVYLIQLSREVRPELLDGVELRGRGWGDVVRKALDRDEGSGSGGEMEDAHYLKAVRALKDSAQLWKDQEEFYLTAAVKFAWEFEKWSFGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5G667</accession>
    <name>D5G667_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00001632001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430008">
        <property type="protein sequence ID" value="CAZ80010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835853.1">
        <property type="nucleotide sequence ID" value="XM_002835807.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G667"/>
    <dbReference type="GeneID" id="9187689"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001632001"/>
    <dbReference type="HOGENOM" id="CLU_2225116_0_0_1"/>
    <dbReference type="InParanoid" id="D5G667"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ80010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="106" mass="12641" checksum="73E8D58A5C3D3552" modified="2010-06-15" version="1">MHLAYTSCWAKGPMVDRILKEIWVPPQWWRGPHPGYRPLRRDAIIIDQEIEWVVENRVRQEPGAYGFIVFDEDIFCPRYRQLRRPAQLPTTFTSCAKNLTQETLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5G673</accession>
    <name>D5G673_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Dynein light chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00001641001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Acts as one of several non-catalytic accessory components of the cytoplasmic dynein complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in changing or maintaining the spatial distribution of cytoskeletal structures. Also a component of the nuclear pore complex.</text>
    </comment>
    <comment type="function">
        <text evidence="11">Acts as one of several non-catalytic accessory components of the cytoplasmic dynein complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in changing or maintaining the spatial distribution of cytoskeletal structures.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Cytoplasmic dynein consists of two catalytic heavy chains (HCs) and a number of non-catalytic subunits which present intermediate chains (ICs), light intermediate chains (LICs) and light chains (LCs).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
            <location evidence="1 11">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the dynein light chain family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430008">
        <property type="protein sequence ID" value="CAZ80016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835859.1">
        <property type="nucleotide sequence ID" value="XM_002835813.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G673"/>
    <dbReference type="FunCoup" id="D5G673">
        <property type="interactions" value="620"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G673"/>
    <dbReference type="GeneID" id="9187695"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001641001"/>
    <dbReference type="eggNOG" id="KOG3430">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070944_4_0_1"/>
    <dbReference type="InParanoid" id="D5G673"/>
    <dbReference type="OMA" id="THEKGHF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990429">
        <property type="term" value="C:peroxisomal importomer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008574">
        <property type="term" value="F:plus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048468">
        <property type="term" value="P:cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040001">
        <property type="term" value="P:establishment of mitotic spindle localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030473">
        <property type="term" value="P:nuclear migration along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051292">
        <property type="term" value="P:nuclear pore complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043935">
        <property type="term" value="P:sexual sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd21452">
        <property type="entry name" value="DLC-like_DYNLL1_DYNLL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.740.10:FF:000001">
        <property type="entry name" value="Dynein light chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.740.10">
        <property type="entry name" value="Protein Inhibitor Of Neuronal Nitric Oxide Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037177">
        <property type="entry name" value="DLC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019763">
        <property type="entry name" value="Dynein_light_1/2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001372">
        <property type="entry name" value="Dynein_light_chain_typ-1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11886">
        <property type="entry name" value="DYNEIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11886:SF35">
        <property type="entry name" value="DYNEIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01221">
        <property type="entry name" value="Dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01375">
        <property type="entry name" value="Dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54648">
        <property type="entry name" value="DLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01239">
        <property type="entry name" value="DYNEIN_LIGHT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6 11" id="KW-0243">Dynein</keyword>
    <keyword evidence="5 11" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="7" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="7" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0811">Translocation</keyword>
    <keyword evidence="11" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00055833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU365010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ80016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="97" mass="10849" checksum="5A9612A8921A57B1" modified="2010-06-15" version="1">MATAPPSTTPEPKTTIKSVDMTEEMSSIAIDVAKKAINSNNVEKDIAQYIKKEFDTKFGSTWHCIVGRNFGSFVTHETKNFIYFYVGHLAVLLFKTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>D5G680</accession>
    <name>D5G680_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00001648001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430008">
        <property type="protein sequence ID" value="CAZ80023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835866.1">
        <property type="nucleotide sequence ID" value="XM_002835820.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G680"/>
    <dbReference type="STRING" id="656061.D5G680"/>
    <dbReference type="GeneID" id="9187702"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001648001"/>
    <dbReference type="eggNOG" id="ENOG502QWIY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016622_2_0_1"/>
    <dbReference type="InParanoid" id="D5G680"/>
    <dbReference type="OMA" id="NQPEFYT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070086">
        <property type="term" value="P:ubiquitin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22952">
        <property type="entry name" value="ART10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011021">
        <property type="entry name" value="Arrestin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050357">
        <property type="entry name" value="Arrestin_domain-protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188:SF166">
        <property type="entry name" value="ARRESTIN (OR S-ANTIGEN), N-TERMINAL DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_7G02050)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00339">
        <property type="entry name" value="Arrestin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arrestin-like N-terminal" evidence="2">
        <location>
            <begin position="3"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="401"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="407"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="447"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="466"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ80023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="537" mass="58768" checksum="EAE18A24AEA6BDDA" modified="2010-06-15" version="1">MSVRIELDNRGSLFTCLDYVTGKVVMNIANDETISAITVKMEGISRTRLTPPRNDEGRDSKHAEIENHRLLYLVQTVFPSPEIRENTSATNSGYTLKPGQYEYPFKLRIPVNSQCTEGQGVGGNILQRLSFDKGTADIARDARRHHPGTLPPSLSGIPGDEALIRYFLKVTVIRPEFYKFNLRAIDPFVFLPIEPPRSPPTTNETYARRQHQFIYNVSPVSKKRGLFSPFRQQAPAINTLSRAVHFSIEARLPSPAIIVPNIQLPLRVILVKIEPYTTPVFIRSLQIHLFAFTGITAHGLGKTDKQIITCLSLMGLQSPIGDDYQAIGKEVEADQALWANAALPDSVPPSFRTCNIRRTYELEIILGLSHGRSGPTEMVPLTLPIKVYSGIKPPPGLLETARHPKTPQFPPLPTKEPVPPLPPRTPTTQSLKVPTAAFGGYNSTKNSPVSSPVSPSFPARPHSSSCGGGGWGGGNGGGGAWPGPRPSSVSSVFPEDDIPPPTYEDTIAEDIGPVDGPRRRYEQEGAYYGALPDDVHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5G688</accession>
    <name>D5G688_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00001657001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430008">
        <property type="protein sequence ID" value="CAZ80031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835874.1">
        <property type="nucleotide sequence ID" value="XM_002835828.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G688"/>
    <dbReference type="GeneID" id="9183486"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001657001"/>
    <dbReference type="HOGENOM" id="CLU_2499505_0_0_1"/>
    <dbReference type="InParanoid" id="D5G688"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="86" mass="9171" checksum="C2D5188236259DB8" modified="2010-06-15" version="1">MGVRVIRVGGVKIMMDGIRSRIYGRDSLSLEGVEGSDPLGAGMAQPLFYAARPTSMVGGGVLSDNSNETSAQWQTDNVGNDNRNRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6C1</accession>
    <name>D5G6C1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00004418001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic initiation factor 4G family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430009">
        <property type="protein sequence ID" value="CAZ80064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835907.1">
        <property type="nucleotide sequence ID" value="XM_002835861.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6C1"/>
    <dbReference type="FunCoup" id="D5G6C1">
        <property type="interactions" value="597"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G6C1"/>
    <dbReference type="GeneID" id="9187435"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004418001"/>
    <dbReference type="eggNOG" id="KOG0401">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003998_0_0_1"/>
    <dbReference type="InParanoid" id="D5G6C1"/>
    <dbReference type="OMA" id="IANQSQW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016281">
        <property type="term" value="C:eukaryotic translation initiation factor 4F complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000020">
        <property type="entry name" value="Eukaryotic translation initiation factor subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.970.30:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor subunit eIF-4F, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.970.30">
        <property type="entry name" value="eIF4G, eIF4E-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022745">
        <property type="entry name" value="eIF4G1_eIF4E-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036211">
        <property type="entry name" value="eIF4G_eIF4E-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003890">
        <property type="entry name" value="MIF4G-like_typ-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23253">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4 GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23253:SF9">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4 GAMMA 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12152">
        <property type="entry name" value="eIF_4G1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02854">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00543">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101489">
        <property type="entry name" value="Eukaryotic initiation factor 4f subunit eIF4g, eIF4e-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="MIF4G" evidence="9">
        <location>
            <begin position="636"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="33"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="132"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="440"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="506"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="904"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="35"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="86"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="144"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="178"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="335"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="440"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="457"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="474"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="506"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="529"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="540"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="608"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="904"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="947"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="977"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1008"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1041"/>
            <end position="1052"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ80064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1068" mass="116974" checksum="6DD50538840F4B9A" modified="2010-06-15" version="1">MPGQMPGHMAYPMDPNYYSGGYMYHHPATPGYGNVPQTSPRPSFQPMSSPVPPYLQPFTPGAPMSRTPSTNMEMHSARPNSSLGQPPTPSHTPQPINMQPPTPQHPSNTYAYPQKKRTHKVEIKNPVTGSVVHYDEVKPPSTTPPVVTTAPLIVSSPSPAPGRTLSRDSPHTRPASKVKSEQEKREEMKVSVQKRMEEQRKQEEQMLEKQRLEAEKVAKEEQERLTKEAEEKKRREEEERLRKEEEERLRKEAEERALKEEEERRREAEKAEAARIAREKEEEAAREAARVAKEKEEEEARIKAEAEKKAKEEEEAARVKAEAEAKAAEALKASSAPTISKTFTPDNETMPPPASRVVGGSKPKPSPLNLQIKTNEPGPPSAALTSLRSARFIEDINSVNYPANILSPNPALNPAASGKFKYPKDFLMQFKDVFTEKPSVDWDQKMRETVGEPDSARPQTARNSGGSMAPRGSTPRTAASSAQPPMGAMGTFGSFKTAAASMITSNRGFERSSTTGSLPPGGLIFNPRSGGGGGGGGSGLRRTGSSTSLSGGNTPASPRTGNRSSRGSSRKGGGAPMEKTESKQGSGSQPTIPLAEVKPLPISENRWKPPSATKAAESAAASTPSEDNRLSPELVQRKVKAALNKMTPEKFDKISDQILEITSQSKYETDGRTLRQVIQLTFEKATDEAAWSSMYAKFCKRMLEAMDPSIKDENIKDRNDNLVTGGSLFRKYLLNRCQEEFERGWKVNLPPKPEGVSDEAAMLSDEYYIAAAAKRRGLGLVQFIGELFKLGMLTERIMNECVRKLLDFEGVPEDETVESLVKLLRTIGRALDASEKSKNMMDMYFSRIKLLIGDKDLNSRMRFMLMAIITNGAQDVIDLRSKEWETKETDKGPKTIQEVREDALKAQQEKEAATRANQRSRPGGGRGGDTRSFSGGGYGNIHHQGGWQDNSKVNTSDLEKLSSRARPSNRGPSTFGPPSMFSRSTSGSGSRRGLGPSISRGPEDSHSSSRTATPAPPTSSKNPFSPLGESHHDSGEINEATSPPSTTSSPPTANSRIRSPPLMKEEAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6Q5</accession>
    <name>D5G6Q5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphatidylinositol 4-kinase</fullName>
            <ecNumber evidence="7">2.7.1.67</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00002170001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19877"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58178"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.67"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="7">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Vacuole membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the PI3/PI4-kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.67" evidence="7"/>
    <dbReference type="EMBL" id="FN430012">
        <property type="protein sequence ID" value="CAZ80198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836041.1">
        <property type="nucleotide sequence ID" value="XM_002835995.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6Q5"/>
    <dbReference type="FunCoup" id="D5G6Q5">
        <property type="interactions" value="241"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G6Q5"/>
    <dbReference type="GeneID" id="9187224"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002170001"/>
    <dbReference type="eggNOG" id="KOG2381">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009049_2_0_1"/>
    <dbReference type="InParanoid" id="D5G6Q5"/>
    <dbReference type="OMA" id="NWMVKVD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004430">
        <property type="term" value="F:1-phosphatidylinositol 4-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039756">
        <property type="entry name" value="Lsb6/PI4K2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12865:SF1">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE TYPE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12865">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE TYPE-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="9">
        <location>
            <begin position="160"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="36"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="305"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="586"/>
            <end position="655"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ80198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="692" mass="78145" checksum="ABC13E90C4337E60" modified="2010-06-15" version="1">MPRLAASGYERLAQEAQLSDSDDDDRESIVDHPGRVVTVQPRPPTSMLSTPYGPVTPSHNGRSMKRLRSNSSGVDIKAINARLERWSHEIANKFKFKKGRSQQEHPPLEIVYSVFVPPEGTRPLTDASPLGLSPVAENIQFTQDQFEEIVESVRLAIRKGIDPKLIKQGSSGSYFMRDSDGNVVGVFKPKDEEPYGKLNPKMMKWLHRTLFPCFFGRACLIPNLSYISEAAACLLDRQLKTFLVPYTDVVKLSSKSFHYDYWDRRAYYKKHKPLPSKVGSFQVFLKGFQDANVFLRKHPWPDQFNSSGAVDDDSDEESHTPLSVDDPDNRRFVWTEQLQQNFREELEKLVILDYIMRNTDRGLDNWMIREPMVASSRSETPQPGAGAALTIGAIDNSLAFPWKHPDQWRSFPFGWLFLPVSLIGQPFSQKTRDHFLPILTSAKWWAETQTLLRRLFEQDSDFKERMFQKQIAVLKGQAFNVVETLKTPDQGPLELTRRTRVHVWDDEMEVPVAVPLRVPTTSDARHRRGHDLERGFNEQVEMDIGAAASSAPLSREIDILGLGLSPPRYEIPSSVRLGISRTTSIGSRRAGIRSGAPGSPRPQRLDYKSFDAGSRNGPSRRKDAPGLLDPRRRRFSLTGGLGGGPPRNVYNDDDAEGDLGYSAVGGSESAQRKVIVERLEAVKSRNPVFTWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7H5</accession>
    <name>D5G7H5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00002468001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430027">
        <property type="protein sequence ID" value="CAZ80468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836277.1">
        <property type="nucleotide sequence ID" value="XM_002836231.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7H5"/>
    <dbReference type="STRING" id="656061.D5G7H5"/>
    <dbReference type="GeneID" id="9188493"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002468001"/>
    <dbReference type="HOGENOM" id="CLU_1788220_0_0_1"/>
    <dbReference type="InParanoid" id="D5G7H5"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040554">
        <property type="entry name" value="KPWE_PEX14_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36855">
        <property type="entry name" value="CHROMOSOME 10, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36855:SF1">
        <property type="entry name" value="PEROXISOME MEMBRANE ANCHOR PROTEIN PEX14P N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17733">
        <property type="entry name" value="KPWE_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peroxisomal membrane protein PEX14-like KPWE" evidence="2">
        <location>
            <begin position="90"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="13"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="23"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ80468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="145" mass="15615" checksum="92A9C8160ED028CE" modified="2010-06-15" version="1">MTGIKVDYNVYKARISQGKPNSTTTTTATATAPENPPEFAQKEHSTPLDSTNGEPILDTTERDVNSEQFPLGNLDPNTPATTSGGTPDAPYPNSFAYIVELITTGQPIPGIKQIPNELNSAPPSEPVRAKRLKPWEKASNDNLNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7Q2</accession>
    <name>D5G7Q2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00004676001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic RPB8 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430031">
        <property type="protein sequence ID" value="CAZ80545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836354.1">
        <property type="nucleotide sequence ID" value="XM_002836308.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7Q2"/>
    <dbReference type="FunCoup" id="D5G7Q2">
        <property type="interactions" value="766"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G7Q2"/>
    <dbReference type="GeneID" id="9187024"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004676001"/>
    <dbReference type="eggNOG" id="KOG3400">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103864_1_0_1"/>
    <dbReference type="InParanoid" id="D5G7Q2"/>
    <dbReference type="OMA" id="KEDDKGW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003968">
        <property type="term" value="F:RNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006363">
        <property type="term" value="P:termination of RNA polymerase I transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006386">
        <property type="term" value="P:termination of RNA polymerase III transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006361">
        <property type="term" value="P:transcription initiation at RNA polymerase I promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000191">
        <property type="entry name" value="DNA-directed RNA polymerases I, II, and III subunit RPABC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005570">
        <property type="entry name" value="RPABC3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10917">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10917:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03870">
        <property type="entry name" value="RNA_pol_Rpb8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000779">
        <property type="entry name" value="RNA_pol_Rpb8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00658">
        <property type="entry name" value="RPOL8c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="95"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ80545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="174" mass="19512" checksum="0E7C9DA11EFF4E49" modified="2010-06-15" version="1">MCLLPFFSTTNLSPHQNLSRTPLISGSTNTMSESILFDSTFPITGFDQNKYDRVARISGEREDLKFSLDIHVELYPVAIGETVQLSLASTLSPDGAKEDPAESKTGWKENKAAEPSLADTYDYVCHGKVYRFEEGNGEFIKVYASFGGLLLFLEGPHKKLSSMRHEYVYLLMKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8B7</accession>
    <name>D5G8B7_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">RING-type E3 ubiquitin transferase (cysteine targeting)</fullName>
            <ecNumber evidence="17">2.3.2.36</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Peroxin-2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">GSTUM_00004727001</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-S-ubiquitinyl-L-cysteine.</text>
            <dbReference type="EC" id="2.3.2.36"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the pex2/pex10/pex12 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.36" evidence="17"/>
    <dbReference type="EMBL" id="FN430042">
        <property type="protein sequence ID" value="CAZ80760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836569.1">
        <property type="nucleotide sequence ID" value="XM_002836523.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8B7"/>
    <dbReference type="STRING" id="656061.D5G8B7"/>
    <dbReference type="GeneID" id="9188362"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004727001"/>
    <dbReference type="eggNOG" id="KOG2879">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024591_2_1_1"/>
    <dbReference type="InParanoid" id="D5G8B7"/>
    <dbReference type="OMA" id="CYVCLAG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016562">
        <property type="term" value="P:protein import into peroxisome matrix, receptor recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025654">
        <property type="entry name" value="PEX2/10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006845">
        <property type="entry name" value="Pex_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48178">
        <property type="entry name" value="PEROXISOME BIOGENESIS FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48178:SF1">
        <property type="entry name" value="PEROXISOME BIOGENESIS FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04757">
        <property type="entry name" value="Pex2_Pex12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Pex N-terminal" evidence="19">
        <location>
            <begin position="86"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="407"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="418"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="452"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF04757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CAZ80760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="490" mass="54558" checksum="3E9E8508EB704A9E" modified="2010-06-15" version="1">MATITATAPTAATTTTTTAATAISVNPPTDWQLAHQHAQQRITRLQEQRAREASGQNRVASFFGGRSVVEGRPAFRVGQLDTELLDEELLELMKGQLWNGLKYFRPSLKEAYEPEFLLLLRAALFKLTIWDHNATYGAALQNLVYVDARSKGPIDQPPTALQKSLYGLITVGGRYMFSRMNLFLLSRSNPEDSTPFTERLSNLVESLSTLHSGLTLLSFTAFLVTGHYRTLLDRALRMRLVSPTRLVSREVSFEYLNRQLVWHAFTEFLLFILPLIRVSRWRRWWARLDRKIRSSSGAATTSTGEGYGELNFLPECTCAICHKETDGGAEATGGKATDVVNPYEAVGCGHIYCYVCLAGKIELEEGDGWTCLRCGNLVKRCRPWRGGLKGLTTVGDWGEEVVDRRRVGFGENGPDSGPEIDTEDLETTASDDDDGEGTERADEEVSDIGSEDTERPNRGRPGFKEKHIAGLWAEETLDESVYNTAEGEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8D5</accession>
    <name>D5G8D5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00004764001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 10">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the oligosaccharyltransferase (OST) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Endoplasmic reticulum membrane</location>
            <topology evidence="2 10">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the OST1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430042">
        <property type="protein sequence ID" value="CAZ80778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836587.1">
        <property type="nucleotide sequence ID" value="XM_002836541.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8D5"/>
    <dbReference type="FunCoup" id="D5G8D5">
        <property type="interactions" value="1059"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G8D5"/>
    <dbReference type="GeneID" id="9185998"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004764001"/>
    <dbReference type="eggNOG" id="KOG2291">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031381_1_0_1"/>
    <dbReference type="InParanoid" id="D5G8D5"/>
    <dbReference type="OMA" id="RYEYARE"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018279">
        <property type="term" value="P:protein N-linked glycosylation via asparagine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007676">
        <property type="entry name" value="Ribophorin_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21049:SF0">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21049">
        <property type="entry name" value="RIBOPHORIN I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04597">
        <property type="entry name" value="Ribophorin_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0732">Signal</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005126574" description="Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1" evidence="10">
        <location>
            <begin position="27"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="458"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ80778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="483" mass="55008" checksum="114984F6DBE99A4E" modified="2010-06-15" version="1">MRFLRNWGALLLPLASLFSLAPCSSADSTLVQPKEFTPPPVFKNTNLLRTIDITKPYVRETTAMVIDNISKEPQTEYYVLVPKGIIENISYFEARGESGPHAVSPTVFNPKSETQYYRVLFSAPLPAGKSAKLWITLAQTDCLTPVPALIGQTDKQFLQWTGSQYAASAYITEKQKTKIKFPNNEVPNFTVLENNDENDDPTGAGSWYTYGPYKTVNPDQKGGKAITLRFEYTSPVIKMIRMERHIEVSHWGGNIAFQERYWMTNLGAKLKDTFSRVQWASTSYYNPPTSAIKQLTFTLIPGAQDAYFTDEIGNVSTSRFRSNMREAHLELKPRYPVFGGWNYSFVVGWNHDLKNFLRVSQDGERYILKVPFLEGPKDSVTYDEMEITVVLPEGAIDVKYSPPIPLISEEEYLHHTFMDTLGRTAIKMRAVNVVDEQHKKELIVTYHYPTMALHRKPFAIFTGILTLFTFSWLVSKIDLRIGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5G906</accession>
    <name>D5G906_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Type 1 phosphatases regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00004926001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 3">Regulator of type 1 phosphatases which maintains protein phosphatase activity under strict control.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the YPI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430053">
        <property type="protein sequence ID" value="CAZ80999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836808.1">
        <property type="nucleotide sequence ID" value="XM_002836762.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G906"/>
    <dbReference type="STRING" id="656061.D5G906"/>
    <dbReference type="GeneID" id="9181282"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004926001"/>
    <dbReference type="eggNOG" id="KOG4102">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098333_0_2_1"/>
    <dbReference type="InParanoid" id="D5G906"/>
    <dbReference type="OMA" id="RRHIQWA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008157">
        <property type="term" value="F:protein phosphatase 1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004865">
        <property type="term" value="F:protein serine/threonine phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011107">
        <property type="entry name" value="PPI_Ypi1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20835:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PPP1R11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20835">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PPP1R11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07491">
        <property type="entry name" value="PPI_Ypi1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="78"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="17"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="85"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="105"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU367162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ80999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="148" mass="16279" checksum="127227AD8198C21A" modified="2010-06-15" version="1">MLLPPPDRRELAPALPSATQTETDPSVQTSIRSVRIPDGTLRLRGGPVENRRVRWDAGVIDNEGMGKKKSKVCCIYSRPRAYDESSSDESDSSSDEDSENEGHGHTHNKNCGHGPKRSAKGKSRDSKRAPNPNAYEKMPKPARKKSAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9S6</accession>
    <name>D5G9S6_TUBMM</name>
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        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">GSTUM_00005053001</name>
    </gene>
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        <lineage>
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            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
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            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
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                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002837032.1"/>
    </dbReference>
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    <dbReference type="KEGG" id="tml:GSTUM_00005053001"/>
    <dbReference type="HOGENOM" id="CLU_2499507_0_0_1"/>
    <dbReference type="InParanoid" id="D5G9S6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="86" mass="9214" checksum="7A635E99B5351F2C" modified="2010-06-15" version="1">MPSPYAEELKIGNFDISLITSIKLKLSLCSRARVYIGSQVPGNRGDKSGQTQPNTDPFCSAPAHPISLLIGDEPMYGTRLVPAGPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>D5GA27</accession>
    <name>D5GA27_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00003542001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
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                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="CAZ81370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002837133.1"/>
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    <dbReference type="STRING" id="656061.D5GA27"/>
    <dbReference type="GeneID" id="9187068"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003542001"/>
    <dbReference type="eggNOG" id="ENOG502QV88">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011273_0_0_1"/>
    <dbReference type="InParanoid" id="D5GA27"/>
    <dbReference type="OMA" id="VMWLKKV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010972">
        <property type="term" value="P:negative regulation of G2/M transition of mitotic cell cycle"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052945">
        <property type="entry name" value="Mitotic_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43628">
        <property type="entry name" value="ACTIVATOR OF C KINASE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43628:SF11">
        <property type="entry name" value="PROTEIN DSF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08238">
        <property type="entry name" value="Sel1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="200"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="583"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="673"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="258"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="277"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="306"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <begin position="375"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="395"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="427"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="469"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="517"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="554"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="583"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="675"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="751"/>
            <end position="769"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="973" mass="105195" checksum="ACB097895975AE3F" modified="2010-06-15" version="1">MPPPTNHAAGEYTPQVSPLDDLINRGRMLQKQMGSARAGSAGLNPMTVHRSLSRAGSNPGTLGTLARSRSVGRTPEVEKGGQWGNTAVTATSAATTPILKVMGDRQLSTPALEDRPTSSYPRFSTVSSATSDDGRSDRDSIMSGETSVRDSNFTLISGFLNPVRDPPNLQQIQGRLEVREQDVERSSLKPVIVGRIEDERRQREAEGVGSVPGGVGARGSPRVGNEPRGAIAQRRAPEGRQRGPSGPLGPTGPSGPEGRQRNPGERQRGPPGPPGPDGRRRVPDGRQHSPGQRQRGPPGPGGRQYGPDERQRGRGQDQRDTAGQGNASEYARSRSQPPPRNRRPPGRQPLEAEQQFPVRKDSRDLTVLIPPGPSGTHTQPSQPAQAYTPYRQDSKSSPLGYSAPSRQGSAESEKFPPPMRQASGERLYPHRNDSRGSDRRFYPRSPRREDSNDSVTKYLPYRQGSGDVEFQRPSRTDSLESVHRSYSPQLAPVPRTASPAQVPSSIFPMISDFRASSPDSIGAESSLTFGPPSAPLPRNPSVPYGGTDSHDMTRSASQSSLTSDAPSVHSVGGILLQKPTFNFNFSRPLSTRPSFESERLSPSPDVPQRLSPERSPMLLDEQSLPTPVSMSGDFTEDAGGPVPTYIYSKFILPRGREPERNSVIFQGAAGYPSQVVHQQTHSPSTPLGNSTTTVDRPPSPSSPPRTFLQPAPPPRDPRHSPHTGHPPSLSPRIGFLTPTVETRGRSAAVTSPCSPRAPNSRSNSSQPPSATMPPDEHVQLGIDLHESGSLQESTYHLRLAANAGHPTGMLLFALACRHGWGMKANQKEGVMWLKKVTELASSEVADDEQAGGSVDYIEKKGRRAQFALSIYELAVSHMNGWGTEKDQGLALRCFEIAGRWGDPDALSEAGFCYANGVGCKKDLKKSAKFYRMAEAKGVNMVGNSWIWKDKYLDEEEKAVKKAVKKNGGGGKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5GA53</accession>
    <name>D5GA53_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00005136001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MIP18 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430075">
        <property type="protein sequence ID" value="CAZ81407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837216.1">
        <property type="nucleotide sequence ID" value="XM_002837170.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GA53"/>
    <dbReference type="FunCoup" id="D5GA53">
        <property type="interactions" value="393"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GA53"/>
    <dbReference type="GeneID" id="9187105"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005136001"/>
    <dbReference type="eggNOG" id="KOG3381">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075876_1_0_1"/>
    <dbReference type="InParanoid" id="D5GA53"/>
    <dbReference type="OMA" id="NQCISAR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.130:FF:000004">
        <property type="entry name" value="cytosolic iron-sulfur assembly component 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="entry name" value="Fe-S cluster assembly (FSCA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039796">
        <property type="entry name" value="MIP18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002744">
        <property type="entry name" value="MIP18-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12377:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR ASSEMBLY COMPONENT 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12377">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR ASSEMBLY COMPONENT 2B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01883">
        <property type="entry name" value="FeS_assembly_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="Fe-S cluster assembly (FSCA) domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIP18 family-like" evidence="4">
        <location>
            <begin position="68"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ81407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="192" mass="21087" checksum="E2DAFBDEDA0A2F7A" modified="2010-06-15" version="1">MDGKNANPTILNASDLPTRRQAQQKKVTSPLTCGLVPAYAQDPFVGNNVEFSDPDESQDDSVVEPIDEQEIYDLIATISDPEHPLTLGELAVVQLQHIRVTDDPSEIISNVLVELTPTINHCSLATVIGLGVRVRLEQALPPRFRLDVRIREGTHATGQQVNKQLNDKERVASALENETLMGVVGKMLETCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAA7</accession>
    <name>D5GAA7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00005206001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430075">
        <property type="protein sequence ID" value="CAZ81461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837270.1">
        <property type="nucleotide sequence ID" value="XM_002837224.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAA7"/>
    <dbReference type="FunCoup" id="D5GAA7">
        <property type="interactions" value="387"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GAA7"/>
    <dbReference type="GeneID" id="9185743"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005206001"/>
    <dbReference type="eggNOG" id="KOG0714">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017633_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAA7"/>
    <dbReference type="OMA" id="FPDVINP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006413">
        <property type="term" value="P:translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10747">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000002">
        <property type="entry name" value="Dnaj homolog subfamily b member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000013">
        <property type="entry name" value="DnaJ subfamily B member 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051339">
        <property type="entry name" value="DnaJ_subfamily_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24078:SF553">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24078">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="3">
        <location>
            <begin position="6"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="76"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="165"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="87"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="105"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="188"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ81461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="373" mass="40907" checksum="12C87D3D1FBC7AA8" modified="2010-06-15" version="1">MVKETKLYDTLGIKPNATPEEIKKAYRKGALQYHPDKNKDSKVAADKFKDISQAYEVLSDPEKRKIYDQFGLEYLLRGGPSSPPPQSQSQSHPSFSRSETFPGGSFPGMGGMGGMGGMGGGGRPYYGASNFHQFGNPESIFEKFARMEGGFEDLGFDILGGLGTGSPLGGGRPSSFPGTRFGGGPREPNGRSKTPESTVSERRVSLTLEELFNGTEKKFRVKRKTFDKDGRISREDKELKIPVKPGMKAGSKFKFKGVGDEIDGSKQDLHFIIEEKPHESFTRDGDDLITTLSIPLKDALLGWSRQIKTIEGKQVKVSHAGPTSPTWQESYPGQGMVLSKTPNERGNLIVKVNIVFPSTLTLEQKNQLRVALP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAL0</accession>
    <name>D5GAL0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00003666001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430079">
        <property type="protein sequence ID" value="CAZ81553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837362.1">
        <property type="nucleotide sequence ID" value="XM_002837316.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAL0"/>
    <dbReference type="STRING" id="656061.D5GAL0"/>
    <dbReference type="GeneID" id="9187493"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003666001"/>
    <dbReference type="eggNOG" id="ENOG502S8IT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_483284_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAL0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="156"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="387"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="458"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="514"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="12"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="54"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="163"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="564" mass="59681" checksum="B4AE284259C9CD77" modified="2010-06-15" version="1">MRSPSYRQNCRCASSTISPPLSSATSTSARARACACSEGESGCDWEFESIASTTPTISTPSHTSASVATPASPPLVLESSRGTLASGIYTPSSHPSTSLSHKKSIIGANAGGFTPAPVEKKFRTLRRRKSRDLHQARRAYLSMSMTTATKLGDTAQQLHPHHTTASTTNSSSTHSPAPPSTVTSCSTAATTPNDSSLSFDEILAYSSPQPISNIPRYAPSDISPRPLSSRSEYSIKTPFSLSAENLSSPYSNDPVTLARPHSMSSLSTPSTVNEQQALHYSIRRSSAPTDTTMFYEPPATRGLSIDYNPWMPNNLTQHSFELPMSSGTSAIKTEPPESWWSEAPKVDIRRLNMIQSQSNEEASASTVARYNDDGSRHSPYGSLLYIHQPPSPAFDDEDSGIYPQSTASPIPSPPESPDFYFPSAVPTPEIGYPPTPSIGRSGSPPVTAAAAAAAALVASKPPKLKSSRPGSCSRRKASAGSMRSQAKSGASPALCIPGETEMAFVNYTPQDKTRILNGVAPSGSSKTKARREREATEKRRRLAAAAAAAVEAAGGDASVLANVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAR9</accession>
    <name>D5GAR9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00003756001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430085">
        <property type="protein sequence ID" value="CAZ81612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837421.1">
        <property type="nucleotide sequence ID" value="XM_002837375.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAR9"/>
    <dbReference type="GeneID" id="9187778"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003756001"/>
    <dbReference type="HOGENOM" id="CLU_2387761_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAR9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ81612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="94" mass="10504" checksum="4DCCA7648B194866" modified="2010-06-15" version="1">MNPDNLAMEDPQILLLPNTILRNAPVSSGVSRYRTSHLTSQFARGSLSYRKTNISLSYSSTVQGFLMSSFSPRHRKLIIKLPNINQIAPRTSSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBP9</accession>
    <name>D5GBP9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Ubiquitin-like modifier-activating enzyme ATG7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Autophagy-related protein 7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00005517001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 and ATG8 for its conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Autophagy is essential for maintenance of amino acid levels and protein synthesis under nitrogen starvation. Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Preautophagosomal structure</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the ATG7 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ81899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837708.1">
        <property type="nucleotide sequence ID" value="XM_002837662.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBP9"/>
    <dbReference type="FunCoup" id="D5GBP9">
        <property type="interactions" value="724"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GBP9"/>
    <dbReference type="GeneID" id="9182936"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005517001"/>
    <dbReference type="eggNOG" id="KOG2337">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012998_2_1_1"/>
    <dbReference type="InParanoid" id="D5GBP9"/>
    <dbReference type="OMA" id="RQIWDAI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019778">
        <property type="term" value="F:Atg12 activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019779">
        <property type="term" value="F:Atg8 activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032446">
        <property type="term" value="P:protein modification by small protein conjugation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01486">
        <property type="entry name" value="Apg7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000243">
        <property type="entry name" value="Ubiquitin-like modifier-activating enzyme ATG7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.100">
        <property type="entry name" value="Ubiquitin-like modifier-activating enzyme ATG7 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.70">
        <property type="entry name" value="Ubiquitin-like modifier-activating enzyme ATG7 N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006285">
        <property type="entry name" value="Atg7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032197">
        <property type="entry name" value="Atg7_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042522">
        <property type="entry name" value="Atg7_N_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042523">
        <property type="entry name" value="Atg7_N_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01381">
        <property type="entry name" value="E1_like_apg7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF3">
        <property type="entry name" value="UBIQUITIN-LIKE MODIFIER-ACTIVATING ENZYME ATG7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16420">
        <property type="entry name" value="ATG7_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0072">Autophagy</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-like modifier-activating enzyme Atg7 N-terminal" evidence="10">
        <location>
            <begin position="1"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="9">
        <location>
            <begin position="299"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="623"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="625"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR606285-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ81899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="643" mass="72187" checksum="FEC0C4DCCD89F560" modified="2010-06-15" version="1">MKYAPFSSLIDLQFYYSLASHKIDYDKLDESPRKVLGQYLPHDGGRMQVHGNALTSDEVPITACRAEGMIKNYNTIEDFRKVEKIVMIKRSAQTIWGAILDGSIYSCPSLLSSFLILSYADLKRYKFHHLFAFPAFAATPQWTCNTPIERLDPHQTSSLVEAINTWRYSTDSRQHGFFLVKRDAGTWKIGSLGDFEHSFFDKDVDEEDRFVGFVDPSDYPDNPGWPLRNLLMLVRKRWGWRKVRVLCLEELNLGDEMPKVVGWEKNDDNKIVPKIANLSAQMDPKKQADINVDLNLKLMKWRVAPELDLDVIRNTRCLLLGAGTLGAYVARNLLSWGVHKITFIDSGTVSFSNPVRQPLYTFQDCIGGETMKAIRAAEALKEIYPGVDAQGFDMTVPMAAHPFSDTQEAGVKQDYLKLRRLFDEHDAIFLLMDTRESRWLPTVMGKSMKKLVMNAALGFDSYVVMRHGVKPSDDQKGGEELGCYYCSDVVAPGDSLVDRTLDQQCTVTRPGVAPIASALLVETLVSVLQHPLGAAAPAPNNDKADRNPPNHPLGLIAHQMRGFLSSFQNIMLKGPAYNCCSACSPAIIKEYRDEGWDFVKKALNQRGYVEEISGLLEVQRRADEADMTDWGDSGDGDEDGDGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBY8</accession>
    <name>D5GBY8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00005647001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ81988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837797.1">
        <property type="nucleotide sequence ID" value="XM_002837751.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBY8"/>
    <dbReference type="GeneID" id="9185423"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005647001"/>
    <dbReference type="HOGENOM" id="CLU_3279817_0_0_1"/>
    <dbReference type="InParanoid" id="D5GBY8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="41" mass="4713" checksum="CA3C94B254E08427" modified="2010-06-15" version="1">MGVGHLFLYWLIGYLIRLVFNLLFKPIVVWAGTSGTPLYEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>D5GC32</accession>
    <name>D5GC32_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00000543001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430099">
        <property type="protein sequence ID" value="CAZ82075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837884.1">
        <property type="nucleotide sequence ID" value="XM_002837838.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GC32"/>
    <dbReference type="FunCoup" id="D5GC32">
        <property type="interactions" value="71"/>
    </dbReference>
    <dbReference type="GeneID" id="9181344"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000543001"/>
    <dbReference type="eggNOG" id="ENOG502QVX4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062645_0_0_1"/>
    <dbReference type="InParanoid" id="D5GC32"/>
    <dbReference type="OMA" id="GRYFQLK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005619">
        <property type="term" value="C:ascospore wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005628">
        <property type="term" value="C:prospore membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059112">
        <property type="entry name" value="CysZ/EI24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052786">
        <property type="entry name" value="Spore_wall_assembly"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34292">
        <property type="entry name" value="OUTER SPORE WALL PROTEIN LDS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34292:SF2">
        <property type="entry name" value="OUTER SPORE WALL PROTEIN LDS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07264">
        <property type="entry name" value="EI24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="167"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="224"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ82075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="283" mass="30923" checksum="645AFC5AE5563846" modified="2010-06-15" version="1">MLLKDTIKTIVRDEVIGVTALAKEPAATGAYLYPLRGIAYFLTHRAIWKPLTSRLLPLLTLSTGVITSMFLFTYIPQSFLLTFVNGPVAWISTIALVLSESAVIITALSRAFLIDEALVDIFDGVLLLEGMSVLVKNGRKLEAGNGVMGKLGALVKKPLEKLTVKSLVSYLIWLPVNFIPIVGTVVFIFVQGRKLGPTYHARYFQLKSYSNQQKNAFVERNMPAYIGFGTASMLLQLTPIVSILFSYTNTVGAALWAVDIERGNRPAEGKRVGGIVAGGEKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>D5GC39</accession>
    <name>D5GC39_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 9">Mitogen-activated protein kinase</fullName>
            <ecNumber evidence="1 9">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00000551001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="9">Activated by threonine and tyrosine phosphorylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="1 9"/>
    <dbReference type="EMBL" id="FN430099">
        <property type="protein sequence ID" value="CAZ82082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837891.1">
        <property type="nucleotide sequence ID" value="XM_002837845.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GC39"/>
    <dbReference type="FunCoup" id="D5GC39">
        <property type="interactions" value="547"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GC39"/>
    <dbReference type="GeneID" id="9181351"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000551001"/>
    <dbReference type="eggNOG" id="KOG0660">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_181_1_1"/>
    <dbReference type="InParanoid" id="D5GC39"/>
    <dbReference type="OMA" id="SFFDFDY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0075018">
        <property type="term" value="P:positive regulation of appressorium formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07849">
        <property type="entry name" value="STKc_ERK1_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000040">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000073">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="19"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ82082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="351" mass="40769" checksum="FF293CF8EF2B75F5" modified="2010-06-15" version="1">MSRHNSSSRKISFNVSDQYEIQDVVGEGAYGVVCSALHKPSGQKVAIKKITPFDHSMFCLRTLREMKLLRYFNHENIISILDIQKPRNYETFTEVYLIQELMETDMHRVIRTQDLSDDHCQYFIYQTLRALKAMHSANVLHRDLKPSNLLLNANCDLKVCDFGLARSAASTEDNSGFMTEYVATRWYRAPEIMLTFKEYTKAIDVWSVGCILAEMLSGKPLFPGKDYHHQLTLILDVLGTPTMEDYYGIKSRRAREYIRSLPFKKRIPFSAMFPKTSASALDLLEKLLAFNPAKRITVEDALKHPYLEPYHDPEDEPTAPPIPDEFFDFDRRKDELSKEQLKMLIFEEIMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCB9</accession>
    <name>D5GCB9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00005809001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430109">
        <property type="protein sequence ID" value="CAZ82162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837971.1">
        <property type="nucleotide sequence ID" value="XM_002837925.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCB9"/>
    <dbReference type="STRING" id="656061.D5GCB9"/>
    <dbReference type="GeneID" id="9184839"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005809001"/>
    <dbReference type="eggNOG" id="KOG1087">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_912743_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCB9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052653">
        <property type="entry name" value="ARF-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004152">
        <property type="entry name" value="GAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47180">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-BINDING PROTEIN GGA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47180:SF1">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-BINDING PROTEIN GGA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50909">
        <property type="entry name" value="GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GAT" evidence="1">
        <location>
            <begin position="165"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="305" mass="33811" checksum="B0913D2EBF382F7D" modified="2010-06-15" version="1">MLSRRLTSHHLRPRASQLAQLISNACSSSISGVRARAKVLEYVRERGGNTTYEAAMALIECVASGGDVAVQAVELLRYCSYSDVPFLATLSQPPLLQPLLILLEERALHACLVELFATLEAAGIGLPYLSTVRKVCNNLRAKGYELSGVLYMKMYSKSELRRRQELAYSARLQACLRRGSKEDLREANGLVKILAGDPEVGGLQQEGEEMLVWKEMIAGVREQVSTLGGALEGKVKREVRYDGFVNKLAHLLQAERRKIDEIEADGNLTESLRAVRREIEEVLQKHRDLVVGGERRVVEGVLVAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCH6</accession>
    <name>D5GCH6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00005895001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430109">
        <property type="protein sequence ID" value="CAZ82219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838028.1">
        <property type="nucleotide sequence ID" value="XM_002837982.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCH6"/>
    <dbReference type="STRING" id="656061.D5GCH6"/>
    <dbReference type="GeneID" id="9186401"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005895001"/>
    <dbReference type="eggNOG" id="KOG0118">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043291_2_1_1"/>
    <dbReference type="InParanoid" id="D5GCH6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12291">
        <property type="entry name" value="RRM1_La"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045180">
        <property type="entry name" value="La_dom_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006630">
        <property type="entry name" value="La_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002344">
        <property type="entry name" value="Lupus_La"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792:SF140">
        <property type="entry name" value="ACHILLES, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792">
        <property type="entry name" value="LUPUS LA PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05383">
        <property type="entry name" value="La"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00302">
        <property type="entry name" value="LUPUSLA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00715">
        <property type="entry name" value="LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50961">
        <property type="entry name" value="HTH_LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="HTH La-type RNA-binding" evidence="7">
        <location>
            <begin position="114"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="252"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="64"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="210"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="342"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="210"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="363"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="379"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="440"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ82219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="491" mass="54439" checksum="F4D8C7A40710DE39" modified="2010-06-15" version="1">MGDEVATDMATEVPNLGENVVEPTVEKGEGVKVVEQPAAPEVGVKQGEPVEENLVEDAVESAVEKDEQVKAESLEQLEDVKSATVEEKDSTTAETKEPPKLQKKSENIKFDATLLPESDDPAEIRKQVEFYFSDSNLPFDKFLFGLVGKDNRSVPLKLIRSFKRMRRFTNPEAIVKALRESEALVITGDIGEETIRRKIPLIINEVENKEGNVESKDSKDKSNHRGYQPTPRQAWDADITGGRKFTDLTIPRSIYAKGFGAETPTTQIDLENFFAPYGPIACVRLRRTRTGFFKGSIFVEFKDEDLMKGFMSLEEKPKWNDTDLQWLTKKAYCDGKIQDIKDGKIQPTLHPPHKSPSNKSGHRGNNQRGNNQRGRGKAGRSGNGGRGGGRHQGSNGRRGNGGKGRNDRGGPPKIKTTPGGVDGIPLVKTNGSASEADEDNNNKRKAENSAEVHTEVKKIKQECDNTYIKQECDNTHIKQECDNTHIKQEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCN0</accession>
    <name>D5GCN0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00000739001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430116">
        <property type="protein sequence ID" value="CAZ82273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCN0"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000739001"/>
    <dbReference type="HOGENOM" id="CLU_3425154_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCN0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="22" mass="2636" checksum="0717307E3D5226AF" modified="2010-06-15" version="1">MKGIALDSRRRKNKNEYQTITL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCP7</accession>
    <name>D5GCP7_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DNA polymerase</fullName>
            <ecNumber evidence="11">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00005938001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">DNA polymerase that functions in several pathways of DNA repair. Involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Also contributes to DNA double-strand break repair by non-homologous end joining and homologous recombination. Has both template-dependent and template-independent (terminal transferase) DNA polymerase activities. Has also a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the DNA polymerase type-X family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="11"/>
    <dbReference type="EMBL" id="FN430120">
        <property type="protein sequence ID" value="CAZ82290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838099.1">
        <property type="nucleotide sequence ID" value="XM_002838053.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCP7"/>
    <dbReference type="STRING" id="656061.D5GCP7"/>
    <dbReference type="GeneID" id="9187909"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005938001"/>
    <dbReference type="eggNOG" id="KOG2534">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008698_4_1_1"/>
    <dbReference type="InParanoid" id="D5GCP7"/>
    <dbReference type="OMA" id="QWITHTL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00141">
        <property type="entry name" value="NT_POLXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.210.10:FF:000005">
        <property type="entry name" value="DNA polymerase IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000010">
        <property type="entry name" value="DNA polymerase lambda"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.110:FF:000005">
        <property type="entry name" value="DNA polymerase POL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.110">
        <property type="entry name" value="DNA polymerase beta, N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.210.10">
        <property type="entry name" value="DNA polymerase, thumb domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002054">
        <property type="entry name" value="DNA-dir_DNA_pol_X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028207">
        <property type="entry name" value="DNA_pol_B_palm_palm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018944">
        <property type="entry name" value="DNA_pol_lambd_fingers_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027421">
        <property type="entry name" value="DNA_pol_lamdba_lyase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037160">
        <property type="entry name" value="DNA_Pol_thumb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022312">
        <property type="entry name" value="DNA_pol_X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002008">
        <property type="entry name" value="DNA_pol_X_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010996">
        <property type="entry name" value="HHH_MUS81"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029398">
        <property type="entry name" value="PolB_thumb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11276">
        <property type="entry name" value="DNA POLYMERASE TYPE-X FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11276:SF29">
        <property type="entry name" value="DNA POLYMERASE TYPE-X FAMILY PROTEIN POL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14792">
        <property type="entry name" value="DNA_pol_B_palm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14791">
        <property type="entry name" value="DNA_pol_B_thumb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10391">
        <property type="entry name" value="DNA_pol_lambd_f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14716">
        <property type="entry name" value="HHH_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00869">
        <property type="entry name" value="DNAPOLX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00870">
        <property type="entry name" value="DNAPOLXBETA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00483">
        <property type="entry name" value="POLXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47802">
        <property type="entry name" value="DNA polymerase beta, N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81585">
        <property type="entry name" value="PsbU/PolX domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8 11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7 11" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 11" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="BRCT" evidence="13">
        <location>
            <begin position="81"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="116"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="116"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU366014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ82290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="598" mass="66968" checksum="56878FCF520FAEF4" modified="2010-06-15" version="1">MSSAPLNLSHLPPTFLLPTRLAQPKANEISSQLRSCKCPLTSNPTDASLFVGDINMPKRCEFELRGRGLSVSSKASPHAKKVRVVKLKWFTESYEGSKLLPIDEYIVWTGYVQPSKRPSTVSSPEQNEGQKSALEKDAADRQLRKEILERARRDQVRYSEEKAHDTRDDIRKLQEMELLYATGRQRKGINELMAISKPGSKNGVRQSTPEYEERREKVVSRGRPRLLKEMPDWIQQRNKYSCCRPTPLTSPNADFISLLDTIKLSRVLTSDGVGIRAYSTAIATLQAYPYPLISPSEVSLLPGCDGKSSALFEEYITSPDHGLAVVRELEKSPSFNILKLFSSIWGVGPVGAREFFYDKGWKTLEDIVDHGWGQLTRAQQIGVKYFDEFQSPISRNEARDIAAVVGKAAAKLRAGMDYTIVGGYRRGKMEIHDVDILLSHTTHSMTTGGVVKELVSELEASGHITHTLHLGRELNNTTTINAHRYGFDSLEKALVVFLAPNSGLHRRVDIILAPPVSVGSALLGWTGGTTFERDLRLWCDKEHGWKFTSEGVFERITGRRVAGVDGTWRVGEKMVEAERRVMEGVGLRWLEPCERCTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>D5GD00</accession>
    <name>D5GD00_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="13">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00006032001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430131">
        <property type="protein sequence ID" value="CAZ82393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838202.1">
        <property type="nucleotide sequence ID" value="XM_002838156.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GD00"/>
    <dbReference type="FunCoup" id="D5GD00">
        <property type="interactions" value="61"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GD00"/>
    <dbReference type="GeneID" id="9183856"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006032001"/>
    <dbReference type="eggNOG" id="ENOG502QRGH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_419897_0_0_1"/>
    <dbReference type="InParanoid" id="D5GD00"/>
    <dbReference type="UniPathway" id="UPA00753"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004608">
        <property type="term" value="F:phosphatidylethanolamine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006656">
        <property type="term" value="P:phosphatidylcholine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007318">
        <property type="entry name" value="Phopholipid_MeTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32138">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32138:SF0">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04191">
        <property type="entry name" value="PEMT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="6"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="155"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="185"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="226"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="254"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ82393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="653" mass="73445" checksum="BC95AFBBD03198C7" modified="2010-06-15" version="1">MGLTAGRWIGGIVVFMFNLWVKLDAHRVVKGYAWYWGDFFFMVDQNLAFDGVFEMAPHPMYSVGYAGYYGISLIVASYPVLFISLAAHAAQFVFLSVVENPHIDKTYNAPAPRRISTPPPTRPLSSDGKESYTAILADDYTHAVKERKPLEKLDMFYNTHLCTALLVFYLAVDAAWVLFRHTLGILLFALQMWTAVSIHELLGEFGWFVVDFFFEQPTPHLTYSGIYRYLNNPELLVGSAGIWGIVLITSSQEIFMLGLFSHVCLLLFIEFVERQHMEKLYGSQIRKEAGVVKTIKEAMPPPVDDGVKSFEGSIDKALADVGEFIEEFLETAKPKLTQGVIGVVKDTQLLISHYPADLSLTFLADGHAKYDAKKYSIAILNALRETNDGIETGLSVEFGQPIRIVWTALVNHLRLDWIGLYRIADNASREVTRIASMGRWTALCKEEYDTDIAEKGIISSDIKTLLPENPNEEILTGELEFGGDKTFWSNGVYEFRYHHDGKHKVIAISLPFEVIINKAPLAADKTKEEHFIDMQEMQESVENYLFPVVVRCFDKEDQDIAPGDVTDGFGGLSDGSMLGGLCTLSRKCLGLISHQRLSKPMEMSNALPGGYAMQREYWQVIYLFISLILQISFQEAPFSMNTPGTLSPFAPER</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDJ1</accession>
    <name>D5GDJ1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="11">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00000964001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MCM10 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430146">
        <property type="protein sequence ID" value="CAZ82584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838393.1">
        <property type="nucleotide sequence ID" value="XM_002838347.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDJ1"/>
    <dbReference type="STRING" id="656061.D5GDJ1"/>
    <dbReference type="GeneID" id="9188175"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000964001"/>
    <dbReference type="eggNOG" id="KOG3056">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011047_0_0_1"/>
    <dbReference type="InParanoid" id="D5GDJ1"/>
    <dbReference type="OMA" id="IGFCKAV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040184">
        <property type="entry name" value="Mcm10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055065">
        <property type="entry name" value="OB_MCM10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015408">
        <property type="entry name" value="Znf_Mcm10/DnaG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13454">
        <property type="entry name" value="PROTEIN MCM10 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13454:SF11">
        <property type="entry name" value="PROTEIN MCM10 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22379">
        <property type="entry name" value="OB_MCM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09329">
        <property type="entry name" value="zf-primase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MCM10 OB-fold" evidence="10">
        <location>
            <begin position="307"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="Zinc finger Mcm10/DnaG-type" evidence="9">
        <location>
            <begin position="447"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="54"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="123"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="169"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="495"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="656"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="92"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="123"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="180"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="201"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="233"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="252"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="294"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="509"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="671"/>
            <end position="684"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF09329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF22379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ82584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="704" mass="76720" checksum="BC6E02CE30D27D5E" modified="2010-06-15" version="1">MDKSNSAFPAACLVKHVARLIDDSPPTSSRTLCVMASNKDLVWPPQSTADVLNLTPRKSRLPNQSIPTSPSPLRKAPLPFLVGGGDGAHDGNDEEEDDEETLQLRLAQIEAKLKLKRLQKKQASQKQASSKSKAVVQVPLSPKSTKTEPVLPKSPSRVLLGIDKGLTGRDVSLRRAPSLRTGSSRPGTASPQKPKKTFSKRIAEERRNGREKAERQRMIESSRSKGFASFGNGPPPSITSGPPPTFGPRGVLQSSGSAATVAQLSKPPSPLASAALEGITDPFTANSKSTPPPEDPDSTDEGGFDSFSGLHLSRRLITHVTLSRHLSPKTIFLLSHLLRSIKSPDFEPPDVLGDWVVMGIICSKSSPRDVGQNTQKRGTGKYMVLQITDLKWEVELFLFGAGFDNFWKVGVGTVVALLNPGILKPRITDTGRFSLTLTEGDQLLEIGQARDLDYCKTVKRDGKRCASWVDKRHTHYCTFHVEQALNKSRVSRAEVNSTTRMFSPPKQKGTLRPRRFFGRGRPNGPFRDDGLLPDVEGGGIADIPQRAGGAGGRVYIAPGRSTASLLDDAEYLADSFLRGSKEDRMRKRLADAQKERELTKRIIKSQGIEGGVGVDYLKKQVRWGEDEIQKRQGEAGVAAASSRNLDDEAANTLSSLRQKAKEATNVSLSPIKRKRPASPAPPKKKIRFAGLDALEEEDDDLVIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDW9</accession>
    <name>D5GDW9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="11">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00006317001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the prenylcysteine oxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430153">
        <property type="protein sequence ID" value="CAZ82712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838521.1">
        <property type="nucleotide sequence ID" value="XM_002838475.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDW9"/>
    <dbReference type="STRING" id="656061.D5GDW9"/>
    <dbReference type="GeneID" id="9184507"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006317001"/>
    <dbReference type="eggNOG" id="ENOG502QSHJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021176_0_0_1"/>
    <dbReference type="InParanoid" id="D5GDW9"/>
    <dbReference type="OMA" id="SIGIWDG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001735">
        <property type="term" value="F:prenylcysteine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030327">
        <property type="term" value="P:prenylated protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030328">
        <property type="term" value="P:prenylcysteine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010795">
        <property type="entry name" value="Prenylcys_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017046">
        <property type="entry name" value="Prenylcysteine_Oxase1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15944">
        <property type="entry name" value="FARNESYLCYSTEINE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15944:SF0">
        <property type="entry name" value="PRENYLCYSTEINE LYASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13450">
        <property type="entry name" value="NAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07156">
        <property type="entry name" value="Prenylcys_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036292">
        <property type="entry name" value="Prenylcysteine_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072301" evidence="9">
        <location>
            <begin position="23"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="domain" description="Prenylcysteine lyase" evidence="10">
        <location>
            <begin position="172"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="31"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ82712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="563" mass="61827" checksum="6057E91DCEBF8120" modified="2010-06-15" version="1">MAIAKSIFLALAALSMTLPVSAHHPGLTHLHNHEGQQPLRDHHNTHDNDGVKRVAIIGAGSAGSSAAYYLRRFHANTTNTPPINITIYERNAYIGGRSTTVNPHGNPGYPIELGASIFVSVNENLVNAAKEFGLTVSSTSSTRPKTVQDSIGVFNGEEMVFVQPYAEGKVGKWWSLAKLLYQYGPFAPIRANRLTDEVVGKFLNMYREPLFPFSSLGEAAESTGLLEATGVTGEQFLMAKGVGNGKFVTELMQSSTRVNYGQNLGLIHGLETMVCMAAEGAVSVEGGNWQIFEGMVKSSGALFHLNTSVTGITEKSTKEDKKIWTITSQEHGDRQPVANQDFDVVIIASPYQYLDLTHPPSTLHKTPDEIPYVALHVTLFTSRRPLDPKYFNLDPASTVPETVLTTLNATEQSSETFTRGEGASAVGNVGFFSISTLQQIFTEEHGEEFVYKVFSPEVFGQDRIRKILGIEGESEDEADVTWVLRHLARTFLPSPSFFFWNAYPYLYPRVTFEDIKLAEGMYYTAGIESFISTMETSSLMGMNVARLVLDEALGARGKVKAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEC8</accession>
    <name>D5GEC8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00001269001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430174">
        <property type="protein sequence ID" value="CAZ82871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838680.1">
        <property type="nucleotide sequence ID" value="XM_002838634.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEC8"/>
    <dbReference type="GeneID" id="9185527"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001269001"/>
    <dbReference type="HOGENOM" id="CLU_2122862_0_0_1"/>
    <dbReference type="InParanoid" id="D5GEC8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ82871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="114" mass="12446" checksum="5F7BE8106BB1C59B" modified="2010-06-15" version="1">MCGVVSVVWVEVSWVGCGVYHLRDASLLFLPTPFCHLCSLTPISPSLPPSIILGCSRSTDERGIPIRRLAVYQKQPGCSCSSTSSGFRWKLSSFCPLAPGTMDTEHTYSTRSSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEM9</accession>
    <name>D5GEM9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00001288001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430179">
        <property type="protein sequence ID" value="CAZ82972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838781.1">
        <property type="nucleotide sequence ID" value="XM_002838735.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEM9"/>
    <dbReference type="GeneID" id="9184885"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001288001"/>
    <dbReference type="HOGENOM" id="CLU_219517_0_0_1"/>
    <dbReference type="InParanoid" id="D5GEM9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="40" mass="4873" checksum="E1AEA97A2356AD53" modified="2010-06-15" version="1">MLLQGLQATNHKRKKKKESNKKHGPYYPKPKKPWKKENRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5GES8</accession>
    <name>D5GES8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00001353001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the actin family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430187">
        <property type="protein sequence ID" value="CAZ83021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838830.1">
        <property type="nucleotide sequence ID" value="XM_002838784.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GES8"/>
    <dbReference type="STRING" id="656061.D5GES8"/>
    <dbReference type="GeneID" id="9184935"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001353001"/>
    <dbReference type="eggNOG" id="KOG0676">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015497_0_0_1"/>
    <dbReference type="InParanoid" id="D5GES8"/>
    <dbReference type="OMA" id="RYIFEKT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="68"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="466"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="601"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="715"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="74"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="475"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="611"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="715"/>
            <end position="738"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ83021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="832" mass="88824" checksum="975E6983A5C1880A" modified="2010-06-15" version="1">MPSFRDDHILVISPGSQVTLAQLGLPESFTPAKLRLASRMFPALESGTWTWTKVREAREGEKDRLLATAMAGHDDDDDDDDDDEEDDEEDDDEEEVEEGDEATAAGTRHEGGSTEGPKASESTIKEVVNAAAEDTAKLDISEKKVAKTEEEGVAIVKDEAKPSGSELKVEDSEMMDEGAVWCMKEGKVVDWGCFFALLQHVHETINPTFHTPILMLCPPAFTPSDKEMITQFVFEKLKAPGFVLMDMALATLWAYGLSTSTVIDVGLEKTDVTPILDFAIQERARETIPGWGGESMTKHLMKLLPELKYEEVEQLKRSPICEILHSGNLLPNATSVNTAIAAGNDKMKRAAEMDGSDLIDEEEGTMNVAAIVASGKTHEFLAKREKEKRGEAERKLPNRERETNSFWVVEKKKHGDEPTSSSAMILDEPQSAVSVSAPKLPEVVVGTEAPPAIPVATVAAETPSASTVPAPEVVPTDAAPITPAAPTAPADSTDSAPTAPPVATSTDEVAATTAVAPIPPVTTEAAAAAGGAIVEKPETEDSAPEPAKPAEIVETTVATEVVATVEATITNTTVTTTATTAPQVTDTTVTVAQETEVAPLPAPAPVVSDEEALRKQREKERRKEEKRAAAEGLPKIGEDEIRREIQVGVERFMAAECGILQELADCIWRLWESLILVGNGSKIRGFKDALLQTIQSKYIIPPSSATIFSSELPSNMSTPAATGTSTPVPQAGPSSHTGANPLLVAATTSNLPLPAIGHHGHHSSHGQTPTNIKFPRAPEYFPEWKDAGFEEAAFLGAQVAAKVLFIVDQGASGGFMTRVEYNEGGPAAIHSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFD7</accession>
    <name>D5GFD7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00006844001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430208">
        <property type="protein sequence ID" value="CAZ83230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839039.1">
        <property type="nucleotide sequence ID" value="XM_002838993.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFD7"/>
    <dbReference type="GeneID" id="9188086"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006844001"/>
    <dbReference type="HOGENOM" id="CLU_2198910_0_0_1"/>
    <dbReference type="InParanoid" id="D5GFD7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="108" mass="12276" checksum="3A1DFACA087C6005" modified="2010-06-15" version="1">MGAAFSRSSTLFNCQISLIRTQKDQPELARPTWIAKNLRPSQKRTKKIRSPRPERNSRVHPLNSRARARVMPDNGPHNNKHEHGNNNTSWGGSGLKRAQQAEICHCNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFI2</accession>
    <name>D5GFI2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="11">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00006911001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the multicopper oxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430208">
        <property type="protein sequence ID" value="CAZ83275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839084.1">
        <property type="nucleotide sequence ID" value="XM_002839038.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFI2"/>
    <dbReference type="STRING" id="656061.D5GFI2"/>
    <dbReference type="GeneID" id="9188223"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006911001"/>
    <dbReference type="eggNOG" id="KOG1263">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006504_3_2_1"/>
    <dbReference type="InParanoid" id="D5GFI2"/>
    <dbReference type="OMA" id="CIAHRYT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13854">
        <property type="entry name" value="CuRO_1_MaLCC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13880">
        <property type="entry name" value="CuRO_2_MaLCC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13901">
        <property type="entry name" value="CuRO_3_MaLCC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000021">
        <property type="entry name" value="Extracellular dihydrogeodin oxidase/laccase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000038">
        <property type="entry name" value="Extracellular dihydrogeodin oxidase/laccase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF71">
        <property type="entry name" value="OXIDOREDUCTASE TPCJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0186">Copper</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072758" evidence="7">
        <location>
            <begin position="18"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="10">
        <location>
            <begin position="69"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="8">
        <location>
            <begin position="193"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="9">
        <location>
            <begin position="399"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ83275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="564" mass="62579" checksum="756524B75EA3F474" modified="2010-06-15" version="1">MHLSLGLTALLAFGVQALPSEIRAREYGGCTHSPTSRHCWDSTTDIHTDYSTTWPNTGVTREYWLILQNVTLAPDGFARTMLTFNGTYPGPPITADWGDMVKIHVTNGLAHNGTSIHWHGVRQLNTFQNDGVNGVTQCPLAPGETQTYTWRATQYGTSWYHSHYAVQYAEGLLGPIIINGPASANYDVDLGAVMVNDWYHNTAFSTWHYAETVGLPQAQNGLIHGQNVFGSGGSRWTTTFIPGKKYRLRFINTGVEAAFRISIDDHKLKVIAADFVPITPYTTDSILIGDGERYDVIVEANFWLRATVQTACSTGVDNPLNIKGIIRYAGGATSGDPTTTGVTIPQNCADEPISSLVPVVPKAVPQVDLSGAQNLFWGLQRTPVIRWTLGGVSTRVDWNHPTLQLLQSTGTYPREYNIVELPEEKWYYFVIEETGVRFAHPLHLHGHDFHILYQGNGTFNATEASLGARWSNPPRRDVAMLPGQGYMVIAFEADNPGIWVLHCHIAWHVSAGFALQFLERQDDIAGVDNSTLNDICYDWSTYQSSATHYQEDSGLKKREFDSIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5GG29</accession>
    <name>D5GG29_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00001966001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430234">
        <property type="protein sequence ID" value="CAZ83472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839281.1">
        <property type="nucleotide sequence ID" value="XM_002839235.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GG29"/>
    <dbReference type="FunCoup" id="D5GG29">
        <property type="interactions" value="369"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GG29"/>
    <dbReference type="GeneID" id="9184535"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001966001"/>
    <dbReference type="eggNOG" id="KOG0730">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_8_3_1"/>
    <dbReference type="InParanoid" id="D5GG29"/>
    <dbReference type="OMA" id="DRHIYVA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19511">
        <property type="entry name" value="RecA-like_CDC48_r2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001721">
        <property type="entry name" value="AAA family ATPase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000012">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF27">
        <property type="entry name" value="ATPASE FAMILY GENE 2 PROTEIN HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="4">
        <location>
            <begin position="132"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="4">
        <location>
            <begin position="417"/>
            <end position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ83472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="651" mass="70373" checksum="3B3A5FA76CD102C4" modified="2010-06-15" version="1">MEGLVESKYIYDNLSIPNTEYKGAKRLFRIEAVTGEGTLVEDRPVVYKATLSTVVEIANPSSPSSTPIILPAPRLNPLKDPISQPGSAIVAPPSKTNFSSIGGLAPQIAALKTLLLSTLHHSHLFTKHNLTPPRGILLYGPPGTGKTLLLKAIASEISAKCYVLNGSVIGKYLGESEAAIRKVFAEARKNQPAVVFMDEADSIACKRGEGDGNEGRIVSTILTEIDGMSCGDSDGVEVVKLVVVATTSRPNVIDQALRRPGRFDREIEIGIPDADSRREILEILTRKIDFSNEQPKEAIIKALAAKTHGFVGADLEELVRTAFTSALTRLERDDLDPSALTLSDTPQTHPQFLLREADLDSALKDVRPTAMREIFLEPPKVRWSDIGGQEEVKQRLREAVEWPLAHPETFSRLGGTPRKGLLLYGPPGCSKTLTAKALATEAGLNFIAVKGPELFNMYLGESERAVREVFRKARAASPSIIFFDEIDALSASRDGGGGGGGGGGKTNVLTTLLNEMDGIEVLKGVTILAATNRPEIIDPALLRPGRLDTILYVGPPDLPAREQILQIKTGKMSISTDVDLGHLAEATEGFSGAEVVNTCDEAIHYAMRESFFIEAVCARHFEAALERAVPQITTEMREAYESWSVGGVKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>D5GG43</accession>
    <name>D5GG43_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00001989001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430241">
        <property type="protein sequence ID" value="CAZ83486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="tml:GSTUM_00001989001"/>
    <dbReference type="HOGENOM" id="CLU_3436980_0_0_1"/>
    <dbReference type="InParanoid" id="D5GG43"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ83486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="12" mass="1473" checksum="6883ECF6ED405B04" modified="2010-06-15" version="1" fragment="single">MTNDREINADYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>D5GG48</accession>
    <name>D5GG48_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007178001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430242">
        <property type="protein sequence ID" value="CAZ83491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839300.1">
        <property type="nucleotide sequence ID" value="XM_002839254.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GG48"/>
    <dbReference type="GeneID" id="9184553"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007178001"/>
    <dbReference type="HOGENOM" id="CLU_102666_0_0_1"/>
    <dbReference type="InParanoid" id="D5GG48"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="179" mass="20708" checksum="9365434B34159590" modified="2010-06-15" version="1">MTTPQPAASSEEEAPFGYTKGGRPRKRPLSDANREGLCPVKECQRSFRHRYGFTLISFLDGNPNIIDSQNPQQALWQHLGYYLSPLHAYFPTSQAHIEMHEKMKAESASRRLTENEKKERQKESKKKWVEKNKEKRALSLKKSNLRKKAKKELKKEGQDLTERAIEELVEKWLVQGGGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>D5GG79</accession>
    <name>D5GG79_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00001991001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430244">
        <property type="protein sequence ID" value="CAZ83522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839331.1">
        <property type="nucleotide sequence ID" value="XM_002839285.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GG79"/>
    <dbReference type="FunCoup" id="D5GG79">
        <property type="interactions" value="370"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GG79"/>
    <dbReference type="GeneID" id="9184584"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001991001"/>
    <dbReference type="eggNOG" id="KOG1337">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030667_1_0_1"/>
    <dbReference type="InParanoid" id="D5GG79"/>
    <dbReference type="OMA" id="IMWAMKV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19180">
        <property type="entry name" value="SET_SpSET10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1410.10">
        <property type="entry name" value="set domain protein methyltransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044432">
        <property type="entry name" value="Set10/Efm1_SET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050600">
        <property type="entry name" value="SETD3_SETD6_MTase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271:SF147">
        <property type="entry name" value="PROTEIN-LYSINE N-METHYLTRANSFERASE EFM1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271">
        <property type="entry name" value="UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ83522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="541" mass="60077" checksum="52730DD163CA3B9C" modified="2010-06-15" version="1">MSARIFSTDEAFNAFGHWLVSHGGFIHGSLTFTPPDSKYGSRVITCTDIPSHSQLISCPHTLTINYTKARSAFSADFITNTTQHAALCMFLCLEWLKGKESFWWPYLCVLPREFDTPLYFSDEDLQFLQGCNLEATEVEARKLIWREEFEAAVSILQREGYDTEYYTWELYLWASTIFTSRSFPGKLMDWDRIIVHEDDTMPILFPLIDSLNHYPATIITWQPSDTSLRIISGVGVSAGAEVYNNYGPKANEELLMGYGFTLLQNPFDSFLLKSSPPLTPLQHSILGESPTGLYHLTPRNRTPENPSIYPSSLLTLIYILTSTPTEASCMLTTQPTKPATKRNELSTHITLLHALLRKHQNFPLTLPEPRNSKQASAKIYADSQRSLILSAIGESKDIINSHTTSSGLIRLQSALGRAGGADMAFAAAIESCFGSADVQELMEAGQEDVVFILYLCHLSLTEKGFRLPVCPTSSPEATQSAQELHEGLFPAVVNAAPAVFGGEGWTVDLLHRGMEAYANMGIRFPEIEGLVVAGEYAVCLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGB4</accession>
    <name>D5GGB4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00007309001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430253">
        <property type="protein sequence ID" value="CAZ83557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839366.1">
        <property type="nucleotide sequence ID" value="XM_002839320.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGB4"/>
    <dbReference type="STRING" id="656061.D5GGB4"/>
    <dbReference type="GeneID" id="9183155"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007309001"/>
    <dbReference type="eggNOG" id="KOG2743">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017452_3_0_1"/>
    <dbReference type="InParanoid" id="D5GGB4"/>
    <dbReference type="OMA" id="QEEFFPV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03112">
        <property type="entry name" value="CobW-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003495">
        <property type="entry name" value="CobW/HypB/UreG_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051316">
        <property type="entry name" value="Zinc-reg_GTPase_activator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13748:SF62">
        <property type="entry name" value="COBW DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13748">
        <property type="entry name" value="COBW-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02492">
        <property type="entry name" value="cobW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobW/HypB/UreG nucleotide-binding" evidence="2">
        <location>
            <begin position="8"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="322"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="323"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ83557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="415" mass="45843" checksum="531992519B7C3417" modified="2010-06-15" version="1">MPSSPPTPITIITGFLGSGKTTLLLKLLPQLPANYGLALLKNEFGDVAVDSRLASQGSISGVKELLNGCICCNLIGQLGNALVTLKTDYKPNRIVIETSGSAFPATLAMEVNRVISENPNFFVLDGVILVIDVENWAGYVDTSYTAKLQARYTDLIVLNKWESVTERREDEVLDRIRDVNEDTPVVKSDKGWVSKDIIFGIDSGLTRELSAATIAQNHEHDEGGGEEHTQEVDVISITLPYRAEEGGKWIDLEALDRFLTSAPKDEVYRIKGSIFSHTKPKSDPPITKDAHRFILNWAFGRWTFTALTKSSVSTVIEAPALRPATSSSANSTAPPSPSEENQRIEVIVQTSIKTTVEQRDQNDDGQEWDGEPVGRFVVITARGEGERWRKRIEKESWIKFDSDILETGLDVVQIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGD4</accession>
    <name>D5GGD4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein uS19c</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Small ribosomal subunit protein uS19m</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00007325001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the universal ribosomal protein uS19 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430264">
        <property type="protein sequence ID" value="CAZ83577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839386.1">
        <property type="nucleotide sequence ID" value="XM_002839340.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGD4"/>
    <dbReference type="FunCoup" id="D5GGD4">
        <property type="interactions" value="110"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GGD4"/>
    <dbReference type="GeneID" id="9183175"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007325001"/>
    <dbReference type="eggNOG" id="KOG0899">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_144911_1_2_1"/>
    <dbReference type="InParanoid" id="D5GGD4"/>
    <dbReference type="OMA" id="EYVAFEV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.860.10:FF:000001">
        <property type="entry name" value="30S ribosomal protein S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.860.10">
        <property type="entry name" value="30s Ribosomal Protein S19, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00531">
        <property type="entry name" value="Ribosomal_uS19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002222">
        <property type="entry name" value="Ribosomal_uS19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005732">
        <property type="entry name" value="Ribosomal_uS19_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020934">
        <property type="entry name" value="Ribosomal_uS19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023575">
        <property type="entry name" value="Ribosomal_uS19_SF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01050">
        <property type="entry name" value="rpsS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880">
        <property type="entry name" value="RIBOSOMAL PROTEIN S19P FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880:SF8">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US19M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00203">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002144">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00975">
        <property type="entry name" value="RIBOSOMALS19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54570">
        <property type="entry name" value="Ribosomal protein S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00323">
        <property type="entry name" value="RIBOSOMAL_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ83577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="96" mass="11148" checksum="8E83F3C4362BC250" modified="2010-06-15" version="1">MFPTRALLKRSIWKGPFVVPYPLQDLSNLEPGKKPLAIRTHVRSCTIIPPFVGQRFQVHNGKDYVEFMVTEEMVGHKLGEFAPTRKKFNYKMSKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGE9</accession>
    <name>D5GGE9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007348001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430264">
        <property type="protein sequence ID" value="CAZ83592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839401.1">
        <property type="nucleotide sequence ID" value="XM_002839355.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGE9"/>
    <dbReference type="GeneID" id="9183190"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007348001"/>
    <dbReference type="eggNOG" id="ENOG502S93Y">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_465410_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGE9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="242"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="311"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="261"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="350"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="367"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="415"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="630" mass="69960" checksum="DE76112AE6E9BA22" modified="2010-06-15" version="1">MEVTVTRAPDAVAPTDTTSPQIPYRLPGCAKSEVSITLAIIPPSRPLTPQQEDEQPASPYPSQTLFPTSPITRTRSGSIASSFDIASDSEAYLNSAATSVTDISLATDTSDIASPDTTIYHIYRNGSAHNNLSIHTVKDSTLKETLQEPDGPPIIIDKGDPISTRISKFKNRKSKLPAKKVKVEDPEMDPRKATYYMHSPMIYWRCPAQTLRFGHNKTAPIVCLVEGGWFWRTWRMDFVVPEKESKKSGKQNRIDSGFAGGEDKGKSGKEWKSLNEPGVIDPRGVLASKYPLRLSGHRKGESGKKYLTERAERQKAKGAPPDQQLKRSKSFSDTARRIFRLNTASPTSKPKGEEKVTTEAKTGDTAEPTTEPTTESTTETTTEPTTEPTTEPTTGSTTRPTREEEIESMLPSGPKPEDLPDHLKPETLEEGSLLMKWSGWLTREYDFKWRGIEFVWKGTGTVRDEKKYWGSLSRLSHLKLVAKFPEEPEEEVEGVTATETKGDKKGPQITIRRQNSFSSFVSTISRKCSNASQLSVSGGGKPRGHREVVVAKYTCLLAKRKCGRLVIYEKALRETTYLTLEGHNPDVARERLRHVVIATGLCMLQAEKQKRDVIKRIIEMGVPEVSNVAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGF4</accession>
    <name>D5GGF4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="11">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00007354001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the arsA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430264">
        <property type="protein sequence ID" value="CAZ83597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839406.1">
        <property type="nucleotide sequence ID" value="XM_002839360.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGF4"/>
    <dbReference type="FunCoup" id="D5GGF4">
        <property type="interactions" value="943"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GGF4"/>
    <dbReference type="GeneID" id="9183195"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007354001"/>
    <dbReference type="eggNOG" id="KOG2825">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040761_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGF4"/>
    <dbReference type="OMA" id="MDAPYEF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043529">
        <property type="term" value="C:GET complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071816">
        <property type="term" value="P:tail-anchored membrane protein insertion into ER membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02035">
        <property type="entry name" value="ArsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000235">
        <property type="entry name" value="ATPase ASNA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03112">
        <property type="entry name" value="Asna1_Get3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025723">
        <property type="entry name" value="ArsA/GET3_ATPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016300">
        <property type="entry name" value="ATPase_ArsA/GET3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027542">
        <property type="entry name" value="ATPase_ArsA/GET3_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00345">
        <property type="entry name" value="GET3_arsA_TRC40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10803">
        <property type="entry name" value="ARSENICAL PUMP-DRIVING ATPASE ARSENITE-TRANSLOCATING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10803:SF3">
        <property type="entry name" value="ATPASE GET3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02374">
        <property type="entry name" value="ArsA_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <keyword evidence="7 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="ArsA/GET3 Anion-transporting ATPase-like" evidence="10">
        <location>
            <begin position="27"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ83597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="333" mass="36899" checksum="F3E62384463B6CC7" modified="2010-06-15" version="1">MSSVFVDSDLDPLEPTLQSILDQKSLRWIFVGGKGGVGKTTTSCSLAIQLAKVRDSVLLISTDPAHNLSDAFNQKFGKEARKIEGFENLSAMEIDPNGSIQELIEGAEGSQNPLGNMMQDLAFSIPGVDEAMSFAEVLKQVKSLSYSTIVFDTAPTGHTLRFLQFPAVLEKALSKISQLSGKFGPMLNGILGAQGGPGGVDEMMQKMEGMREVIGEVSKQFKDPDLTTFICVCIPEFLSLYETERMIQELNNYQIDTHSIVVNQLLFPQKGSTCEQCTARRRMQKKYLEQIEELYEDFNVVKCPLLVEEVRGSDKLRKFSEMLVTPYDAPAED</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGN6</accession>
    <name>D5GGN6_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Inheritance of peroxisomes protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00007438001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for peroxisome inheritance.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Peroxisome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the INP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430275">
        <property type="protein sequence ID" value="CAZ83658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839467.1">
        <property type="nucleotide sequence ID" value="XM_002839421.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGN6"/>
    <dbReference type="STRING" id="656061.D5GGN6"/>
    <dbReference type="GeneID" id="9185580"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007438001"/>
    <dbReference type="eggNOG" id="ENOG502S7ZC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_511104_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGN6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005780">
        <property type="term" value="C:extrinsic component of intraperoxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045033">
        <property type="term" value="P:peroxisome inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024758">
        <property type="entry name" value="Inp1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12634">
        <property type="entry name" value="Inp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="16"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="320"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="388"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="35"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="329"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ83658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="533" mass="57619" checksum="BB9AC61A5EEF65F9" modified="2010-06-15" version="1">MSAAWSQCTPLPRRAVPVPITLRRRSPPGAPLSPSPGSTGSVGGSASASNDQNIDLLFAHSSARIVSFDASASAYAGGQLIPWTSATERTIASGPIRIYKTIPHNIAFLQSGSALRPVLSKSQCWNVDGRGVFCLQIRPRSYWRLEILEDPGEDAIKAIEFKGVLITILAYEITTCPFLRSGGCVTEVNAVKTLEPSRVWRRPRATTLDIEAVREEEPVAAEELPRGVGEVKLAKVEDEHVEAIIGDQEQEGSGNESDTTTLVHDEMDHNISSIDVGPCEEALEGTSTESKGRHDFSLPFRSPRGSMFLSRLSRSCTVPPQLTDTNAFPTISRPSSRSSTPDSNTPISPIEWHTLTPQLPEVRISKRRDRNLSAVGLDLSANLAPEPAQISPALSTPTLWSGSESEDSWSEIATPQRPSLYRNTDSTIAPHSPALSRSTSSLVLSKTAGFVGAAADLVVVKPSSYIAGMMFSIANRIATRAVSGAAYTLAERYGPAVSLGSEEEFDYEDDFGVRLRPGRKDPWDKDVWGPENV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGS4</accession>
    <name>D5GGS4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00007493001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430275">
        <property type="protein sequence ID" value="CAZ83696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839505.1">
        <property type="nucleotide sequence ID" value="XM_002839459.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGS4"/>
    <dbReference type="STRING" id="656061.D5GGS4"/>
    <dbReference type="GeneID" id="9185617"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007493001"/>
    <dbReference type="eggNOG" id="KOG0916">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016425_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGS4"/>
    <dbReference type="OMA" id="NANTAFW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000148">
        <property type="term" value="C:1,3-beta-D-glucan synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003843">
        <property type="term" value="F:1,3-beta-D-glucan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006075">
        <property type="term" value="P:(1-&gt;3)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051278">
        <property type="term" value="P:fungal-type cell wall polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003440">
        <property type="entry name" value="Glyco_trans_48_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12741:SF48">
        <property type="entry name" value="1,3-BETA-GLUCAN SYNTHASE COMPONENT FKS1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12741">
        <property type="entry name" value="LYST-INTERACTING PROTEIN LIP5 DOPAMINE RESPONSIVE PROTEIN DRG-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02364">
        <property type="entry name" value="Glucan_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="307"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="359"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="565"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="606"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="642"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="746"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="799"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase 48" evidence="2">
        <location>
            <begin position="1"/>
            <end position="634"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ83696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="890" mass="100713" checksum="9DDF76AFA2FF626F" modified="2010-06-15" version="1">MARRKFKICVSMQRYAKFKKEEMENTEFLLRAYPDLQIAYLDEEPPLVEGGEPRLYSALIDGHSELMENGMRRPKFRVQLSGNPILGDGKSDNQNHAIIFYRGEYIQLVDANQDNYLEECLKIRSVLAEFEEMTVENVSPYTPGLPPTKFDPVAILGAREYIFSENIGILGDVAAGKEQTFGTLFARTLAQIGGKLHYGHPDFLNGIFMTTRGGVSKAQKGLHLNEDIYAGMNALLRGGRIKHCEYYQCGKGRDLGFGSILNFTTKIGTGMGEQMLSREYYYLGTQLPLDRFLSFYYAHPGFHINNLFIMLSVQLFMFVMIHLGALKDQVVVCDYNPNKPITDELKPIGCRNIEPIMDWVVRCSLSIVIVFFISFVPLVVQELTERGFWRAATRLGRHFCSCSPAFEVFVCQIYANSLLNDLAFGGARYIGTGRGFATARIPFGILYSRFAGPSIYLGARSLMMVLFATLTIWGIHLLYFWASLLALCTSPFIFNPHQFAWDDFFIDYRDYLRWLSRGNSRANHQSWISFCRLSRTRITGYKRKILGDPSEKLSGDMARAKFTNIVFNEIVGPFLVAILCIIPYLFINAQTGDLPEPKPTGALMRLAVVAFAPIAVNAGILAGLFGMACCMGPVFSMCCKKFGGVLAAIAHGLAVIMLFAFFEVMMFLEGFSFTKTLSGMIAMVAIQRWFYKLIISLALTREFKTDQSNLAFWNGKWYGLGLHAISQPGRELLCKITELGMFSADFILGHVILFFQLPVILFPYADRIHSTMLFWLRPSRQIRPPIYSSKQSKLRKKRVIRFTVVYFLMLVVFLALVVGPVVAKNILIDATKSVNIMPDMGLQQPHDTYYNNTDTAYWEANPDLVDDGGKEFREGAHQNKNANSKFKLRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGT6</accession>
    <name>D5GGT6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00007506001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430275">
        <property type="protein sequence ID" value="CAZ83708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839517.1">
        <property type="nucleotide sequence ID" value="XM_002839471.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGT6"/>
    <dbReference type="GeneID" id="9181678"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007506001"/>
    <dbReference type="eggNOG" id="ENOG502SZ4D">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_183945_1_0_1"/>
    <dbReference type="InParanoid" id="D5GGT6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020301">
        <property type="entry name" value="Mrx7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10906">
        <property type="entry name" value="Mrx7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ83708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="73" mass="8523" checksum="CEC710D76E833780" modified="2010-06-15" version="1">MMIRVFEAWLVARLLRSPSFHRMVARMHGRLTGKGQVEDYSKLGGTKLEGGNENGFKKFVDIYIDEFKNNFKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>D5GH53</accession>
    <name>D5GH53_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00002083001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430299">
        <property type="protein sequence ID" value="CAZ83846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839655.1">
        <property type="nucleotide sequence ID" value="XM_002839609.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GH53"/>
    <dbReference type="FunCoup" id="D5GH53">
        <property type="interactions" value="4"/>
    </dbReference>
    <dbReference type="GeneID" id="9187264"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002083001"/>
    <dbReference type="eggNOG" id="ENOG502RYYK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1210561_0_0_1"/>
    <dbReference type="InParanoid" id="D5GH53"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.28.10">
        <property type="entry name" value="Homing endonucleases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027434">
        <property type="entry name" value="Homing_endonucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004860">
        <property type="entry name" value="LAGLIDADG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051289">
        <property type="entry name" value="LAGLIDADG_Endonuclease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36181">
        <property type="entry name" value="INTRON-ENCODED ENDONUCLEASE AI3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36181:SF1">
        <property type="entry name" value="LAGLIDADG ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00961">
        <property type="entry name" value="LAGLIDADG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55608">
        <property type="entry name" value="Homing endonucleases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homing endonuclease LAGLIDADG" evidence="1">
        <location>
            <begin position="76"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="229" mass="26850" checksum="FCA6B2F896B785C8" modified="2010-06-15" version="1" fragment="single">MVSQSNIYLNKNAYVLSFSSKECIIFIVSLINGKMRTPKINALYGLIDWLNNKKSQNSIIHKLPQNSEPLGSNSWLSGFIEGDAHFSVRATLPNKKINYPKVECRIELVQRQIYHNGLSNYNFMADIATFLDCSVKEIRTSSNHPQFRIRTISLKSNEILINYLEKYPLFSKKYLDYKDWLKILEFKKLGKYDQGFLDKVITIKNNMNNKRTFFVWDHLQGFYNLENKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHI0</accession>
    <name>D5GHI0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007925001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430319">
        <property type="protein sequence ID" value="CAZ83973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839782.1">
        <property type="nucleotide sequence ID" value="XM_002839736.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHI0"/>
    <dbReference type="STRING" id="656061.D5GHI0"/>
    <dbReference type="GeneID" id="9183665"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007925001"/>
    <dbReference type="eggNOG" id="ENOG502QSR7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036524_0_1_1"/>
    <dbReference type="InParanoid" id="D5GHI0"/>
    <dbReference type="OMA" id="YDNSHDY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33594:SF1">
        <property type="entry name" value="HD_PDEASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33594">
        <property type="entry name" value="SUPERFAMILY HYDROLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G03035)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD/PDEase" evidence="1">
        <location>
            <begin position="24"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="214" mass="23608" checksum="95EF8CC80578AF6E" modified="2010-06-15" version="1">MVAQDTPLIAAVKEFVNDYMSKYDSSHNYQHIERVLAHSLSILHAAQASPPETHYDENLVILLALLHDVGDKKYSTATTTTSSTAGPAEDFLLSAGASPDLASLLQKLINNLSYSHEQTNPQQVAELVKQHPELAIVQDADRLDAIGAVGVGRTFTFGAIKNQEGGMGAVIRHFDEKLLKLADGMKTVEGRRIARERGERVKAFREWWREEAGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHL6</accession>
    <name>D5GHL6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00007977001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430319">
        <property type="protein sequence ID" value="CAZ84009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839818.1">
        <property type="nucleotide sequence ID" value="XM_002839772.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHL6"/>
    <dbReference type="GeneID" id="9183663"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007977001"/>
    <dbReference type="HOGENOM" id="CLU_2887464_0_0_1"/>
    <dbReference type="InParanoid" id="D5GHL6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ84009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="63" mass="6851" checksum="53A174FFA6A4E84D" modified="2010-06-15" version="1">MATDARLNYSLLNFLIASSTLSSPVVLETGGNHQGVTGIGVVPIRSTPLEYQLAFSFRVRREV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHV3</accession>
    <name>D5GHV3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008132001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430321">
        <property type="protein sequence ID" value="CAZ84096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839905.1">
        <property type="nucleotide sequence ID" value="XM_002839859.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHV3"/>
    <dbReference type="GeneID" id="9184189"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008132001"/>
    <dbReference type="eggNOG" id="ENOG502S75W">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107705_2_1_1"/>
    <dbReference type="InParanoid" id="D5GHV3"/>
    <dbReference type="OMA" id="NEMRGYK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018824">
        <property type="entry name" value="Conidiation-specific_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052670">
        <property type="entry name" value="UPF0654_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36576">
        <property type="entry name" value="UPF0654 PROTEIN C11D3.01C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36576:SF1">
        <property type="entry name" value="UPF0654 PROTEIN C11D3.01C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10346">
        <property type="entry name" value="Con-6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="98" mass="10443" checksum="D904F81DAC297EC6" modified="2010-06-15" version="1">MDSASDQKNLGNVIGGHKANLHNPNTSPEAKQHSREVLEELGFETTSYTSGPSGVDVNIEGKNPGNVAGGYKATLHNPNVSEEAKERAQEKLEQMGAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHW0</accession>
    <name>D5GHW0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00008141001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430321">
        <property type="protein sequence ID" value="CAZ84103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839912.1">
        <property type="nucleotide sequence ID" value="XM_002839866.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHW0"/>
    <dbReference type="STRING" id="656061.D5GHW0"/>
    <dbReference type="GeneID" id="9184196"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008141001"/>
    <dbReference type="eggNOG" id="ENOG502SHSC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_493632_0_0_1"/>
    <dbReference type="InParanoid" id="D5GHW0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1020">
        <property type="entry name" value="Prion-inhibition and propagation, HeLo domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029498">
        <property type="entry name" value="HeLo_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038305">
        <property type="entry name" value="HeLo_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37542">
        <property type="entry name" value="HELO DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14479">
        <property type="entry name" value="HeLo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prion-inhibition and propagation HeLo" evidence="2">
        <location>
            <begin position="5"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="348"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="355"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ84103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="552" mass="59008" checksum="4304573DA9BA7873" modified="2010-06-15" version="1">MESIGVALGIAGLAGLFTTCLQLFDLVSTAESHSSACQTLLCKLEVEQVLLLKWGEAVGIFNPQDSDREERNETYNKRLGDTPIRRAVFNILSCLKTTLEDTNNLVAKYGLQPLQSEDEAFDSSPPRSAFRVAHEKLQRLLKLTQQQASFSRKTRWVVQDKAKFELLIVEIRGFNESLGRLLPEVAMIQQENTRAEVGESSDVAALKTLEEALKGAHDGLSDAASQRLCSLSDAGSPDDIEDRLRQAPPPNACDVTGSTIGLNCVEPQTTAVPKIEKSNLPDTIAEENPDTSKRATASISRRCRCGLETSLENGHVCILKSLQSSPGDYSGTILSRLRSCEDFTATANFNRSNLPSTPSSSSSPTTSSFPGAISSTIPPSQPSTVLLPAIKKSRFHPYQNLSRRNPPTPPRRTVSFDTSRMAPPDSTSNTSSSTPIYQRRYQRPLSRLGSRSPPGFSNTAGGAADYNTGLAVAKSPMTALQDQSMGLAGVKSPMQALIDQESVLAGVKSPMQAITNMGNLARSVDGVHGGPVGYGMALGAGPADDGAGPAGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHY6</accession>
    <name>D5GHY6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00008188001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family. RarA/MGS1/WRNIP1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430322">
        <property type="protein sequence ID" value="CAZ84129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839938.1">
        <property type="nucleotide sequence ID" value="XM_002839892.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHY6"/>
    <dbReference type="FunCoup" id="D5GHY6">
        <property type="interactions" value="783"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GHY6"/>
    <dbReference type="GeneID" id="9184222"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008188001"/>
    <dbReference type="eggNOG" id="KOG2028">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017985_0_3_1"/>
    <dbReference type="InParanoid" id="D5GHY6"/>
    <dbReference type="OMA" id="RIILSQC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051880">
        <property type="term" value="F:G-quadruplex DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051276">
        <property type="term" value="P:chromosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033567">
        <property type="term" value="P:DNA replication, Okazaki fragment processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000731">
        <property type="term" value="P:DNA synthesis involved in DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006282">
        <property type="term" value="P:regulation of DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3710.10:FF:000005">
        <property type="entry name" value="AAA family ATPase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.272.10:FF:000001">
        <property type="entry name" value="Putative AAA family ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000137">
        <property type="entry name" value="Replication-associated recombination protein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3710.10">
        <property type="entry name" value="DNA polymerase III clamp loader subunits, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032423">
        <property type="entry name" value="AAA_assoc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051314">
        <property type="entry name" value="AAA_ATPase_RarA/MGS1/WRNIP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021886">
        <property type="entry name" value="MgsA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13779:SF7">
        <property type="entry name" value="ATPASE WRNIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13779">
        <property type="entry name" value="WERNER HELICASE-INTERACTING PROTEIN 1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16193">
        <property type="entry name" value="AAA_assoc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12002">
        <property type="entry name" value="MgsA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="29"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ84129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="422" mass="46621" checksum="F728527BA8361449" modified="2010-06-15" version="1">MRPKNLDDIHGQELVGRRGVLRGLIEEGKVPSMILWGAAGCGKTTIARVIAAMSSCRFIEISATASGVNDCKKIFIEAKNELVLTGRRTILFCDEIHRYTKAQQDVFLAPVEKGEITLIGATTENPSFKVQSALLSRCRTFALNKLSQADIESILHRALLSESLLDEKKGHTVVSKLLDTSMIEYLARFADGDARAGLNLLELSLGLATSPGITQEQIKKSLAHTIIYDRAGDAHYDTISAFHKSIRGSDPDGAIFWLTRMLKGGEDPLFIARRMIVMASEDIGLADNTMLSLATATFTAIQQVGMPEARINLAHCAVALALSKKSTRSYRALGKAMELYEHDPALANTPVPVHLRNAPTKLMKELVYGKEYKYNPDYKDGKVKQEYLPEQLRDTVFLDDMDLGTKVDPDLKDKQLRVRGSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>D5GI66</accession>
    <name>D5GI66_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008307001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430323">
        <property type="protein sequence ID" value="CAZ84209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840018.1">
        <property type="nucleotide sequence ID" value="XM_002839972.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GI66"/>
    <dbReference type="GeneID" id="9181927"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008307001"/>
    <dbReference type="HOGENOM" id="CLU_761169_0_0_1"/>
    <dbReference type="InParanoid" id="D5GI66"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="128"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="218"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="147"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="219"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="254"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="273"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="290"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="353"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="364" mass="38243" checksum="600CEE9F7848D2DF" modified="2010-06-15" version="1">MSAIIRLRPNRIVLTSDEVHNLNPRNANANGNGSTPSPAATLAWDHNITRRRARARASRKYCAEALDLHAPELKEGSDGSVEILEDEDVRPGWGGGGEVGEGGAGEEVVLEGSGDILVLPFEDVGVRGVQVGEEEEGRVQRTDSDSDSASPRASGSGSSDPTFFAPGAGEVDDASHDFIDIAYLADVDFDLPDSPLPSPPTIEAYLDGATAALASLDISSPATTTAGAKTPTSLRRFAERESSSPGDGVSGLSPRHKAVERKRLARYRDRSHSGKGARLRRSRTAGGGFDGDDEKSDSDNDESSDDRNEDGASSIGEGEDKKGTAGVDASTQEEKVEAGESAMSASERREGNAATTGTCTRNFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIC8</accession>
    <name>D5GIC8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="11">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00008428001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430326">
        <property type="protein sequence ID" value="CAZ84271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840080.1">
        <property type="nucleotide sequence ID" value="XM_002840034.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIC8"/>
    <dbReference type="STRING" id="656061.D5GIC8"/>
    <dbReference type="GeneID" id="9184629"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008428001"/>
    <dbReference type="eggNOG" id="KOG1827">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_467074_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIC8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016586">
        <property type="term" value="C:RSC-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04369">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037382">
        <property type="entry name" value="Rsc/polybromo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054551">
        <property type="entry name" value="RSC4_Ig-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16062:SF19">
        <property type="entry name" value="PROTEIN POLYBROMO-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16062">
        <property type="entry name" value="SWI/SNF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22994">
        <property type="entry name" value="RSC4_Ig_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="3" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Bromo" evidence="10">
        <location>
            <begin position="83"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="33"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="191"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="320"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="508"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="545"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="45"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="332"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="546"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ84271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="584" mass="63478" checksum="573ABA49BD2E6045" modified="2010-06-15" version="1">MCQNAAGYNKPKSQVHSDSMRIRNVVEGFIAEQSNRHVKKEDEASQANHPRQISGRSAKNDPATLQRAQNEIIDELLAFTDEKGESVAHFFINLPSKKLHGEYFRVIKQPISLNLIKRRIVLGEYPTWTEFESAVQLIRSNAEEYNDEQSSIVEDVRKLDTQFHKLLSEAKVRVGNVEGGGSGIRLRLNVHQTQSPPQEPPRGPRIKLNIGSRRESGMDGPSVAAQSSPPPPQRVQRVPKKPPVNGLRTTRAALADRPSPSLESPAIAQANGARSVRGRSNDSPGKPPQGSKKEPTPPTPVTSFLPQAAPRDQKNLLLLPASQSPKPGAGNMTPSTQSGRSRSPANDSITLRARSNPRTPQPRVSSPAPNIAMPPPPQRHSATPVPNGHSPAPQPNASTPAPPALLQPKPNAGPEEPRYRADGKDASAAIISSVRLAAALNGIDGKFSQELFPDDRKLMTQFSIHLPPEAGLVVFTPVLSTANLNNRAYKLTVELRVNNNTPRLLIPNPSLQKKREEPPYEFRPSPGSVSTLECTLVTTVQRPAINGTSNGVNGTTNGTANGTTGTFGNWEMERFRIFFSFLPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIC9</accession>
    <name>D5GIC9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Cyclin-dependent kinases regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00008429001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Binds to the catalytic subunit of the cyclin dependent kinases and is essential for their biological function.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the CKS family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430326">
        <property type="protein sequence ID" value="CAZ84272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840081.1">
        <property type="nucleotide sequence ID" value="XM_002840035.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIC9"/>
    <dbReference type="FunCoup" id="D5GIC9">
        <property type="interactions" value="490"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GIC9"/>
    <dbReference type="GeneID" id="9184630"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008429001"/>
    <dbReference type="eggNOG" id="KOG3484">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140546_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIC9"/>
    <dbReference type="OMA" id="RPINYGQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.170.10:FF:000002">
        <property type="entry name" value="Cyclin-dependent kinases regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.170.10">
        <property type="entry name" value="Cyclin-dependent kinase, regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000789">
        <property type="entry name" value="Cyclin-dep_kinase_reg-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036858">
        <property type="entry name" value="Cyclin-dep_kinase_reg-sub_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23415">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASES REGULATORY SUBUNIT/60S RIBOSOME SUBUNIT BIOGENESIS PROTEIN NIP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01111">
        <property type="entry name" value="CKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00296">
        <property type="entry name" value="CYCLINKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01084">
        <property type="entry name" value="CKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55637">
        <property type="entry name" value="Cell cycle regulatory proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00945">
        <property type="entry name" value="CKS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 5" id="KW-0132">Cell division</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00068939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU311113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ84272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="112" mass="13685" checksum="FDB55AB48A26116A" modified="2010-06-15" version="1">MEKTSARQRRPRLLTDKERDRLEEFVDLIHYSDRYSDKQYEYRHVMLPKQMLKQIPKDYFDTETGTLKILHEEEWRGLGITQSLGWQHYEIHAPEPHILLFKRPKNFQTNAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIG3</accession>
    <name>D5GIG3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00008487001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430326">
        <property type="protein sequence ID" value="CAZ84306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840115.1">
        <property type="nucleotide sequence ID" value="XM_002840069.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIG3"/>
    <dbReference type="GeneID" id="9184664"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008487001"/>
    <dbReference type="eggNOG" id="ENOG502S5M9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073624_2_1_1"/>
    <dbReference type="InParanoid" id="D5GIG3"/>
    <dbReference type="OMA" id="QGIDFCK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36854">
        <property type="entry name" value="CHROMOSOME 9, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36854:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072809" evidence="2">
        <location>
            <begin position="24"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ84306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="132" mass="14592" checksum="A70753597BEB1277" modified="2010-06-15" version="1">MPLPFFSRFFLPILFLLINLAAASGPANIFCKCTCGNNSTLIPITSPPSCSECNRQFCLKQHLPICRDVGEDGIFTTCFQRDSAKDRAVVFIFIVATVGLLGYAGLKGWVGEAIKKWRDRTARERYEALPGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIW2</accession>
    <name>D5GIW2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008711001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840264.1">
        <property type="nucleotide sequence ID" value="XM_002840218.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIW2"/>
    <dbReference type="FunCoup" id="D5GIW2">
        <property type="interactions" value="211"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GIW2"/>
    <dbReference type="GeneID" id="9185673"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008711001"/>
    <dbReference type="eggNOG" id="KOG4203">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066889_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIW2"/>
    <dbReference type="OMA" id="TIHMNDY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006083">
        <property type="entry name" value="PRK/URK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10285">
        <property type="entry name" value="URIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00485">
        <property type="entry name" value="PRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphoribulokinase/uridine kinase" evidence="1">
        <location>
            <begin position="88"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="227" mass="24415" checksum="8B2973781AC1B6B4" modified="2010-06-15" version="1">MPQTTSPILISITGGEAAGKKAVQAGLKSRLLSLNGELKISTLHMADYALPPLLPCTGTAAEGAKRYDLERLAGELEGIVRGDVEEAEVDVVIAEGRYMLCCSRLVEVAGVKIFVDCDSDVRLARKVIRESGTRSLDRILDEYVRKSKPAFEQAILPTKHISDIILPAGAEGPGLDLIAHGIMDDLKRKRGRQSLPGILASVSHLQLPLTLSEVDLTTGAPSYYQTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIW7</accession>
    <name>D5GIW7_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Efflux pump dotC</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Dothistromin biosynthesis protein C</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00008719001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Efflux pump; part of the gene cluster that mediates the biosynthesis of dothistromin (DOTH), a polyketide toxin very similar in structure to the aflatoxin precursor, versicolorin B. One function of dotC may be to transport early-stage dothistromin biosynthetic intermediates from the cytoplasm into vacuoles, thereby affecting the rate of dothistromin production.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. TCR/Tet family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840269.1">
        <property type="nucleotide sequence ID" value="XM_002840223.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIW7"/>
    <dbReference type="FunCoup" id="D5GIW7">
        <property type="interactions" value="53"/>
    </dbReference>
    <dbReference type="GeneID" id="9185678"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008719001"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_22_0_1"/>
    <dbReference type="InParanoid" id="D5GIW7"/>
    <dbReference type="OMA" id="GVCFCHG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17502">
        <property type="entry name" value="MFS_Azr1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1720.10:FF:000014">
        <property type="entry name" value="MFS drug transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000196">
        <property type="entry name" value="MFS toxin efflux pump (AflT)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF102">
        <property type="entry name" value="DRUG TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G08530)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01036">
        <property type="entry name" value="TCRTETB"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="130"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="164"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="222"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="255"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="289"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="321"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="352"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="392"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="428"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="457"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="486"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="519"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="11">
        <location>
            <begin position="132"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00057269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00069956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00083178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ84460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="661" mass="70122" checksum="6B98ADED70A4B070" modified="2010-06-15" version="1">MPPEKAAIQEGEPSTTIPPQAPTAQESLNLRSSAASSSDGATIIVPRTDDFTETNSTSAGSRLGHPFSLNEKLQEYQNSSGIESRGSCGTENAGAVETAAETGVAPDAITEPHAVAPEGQQEGLSQKRKAIIVLALCICVFLAALDQTIITTAMPIIAAQFNSATGYTWIGSSYLLTSAAFLPAWGKLSDIWGRKPVLLSAAMIFLIGSILCAAAPNLGMLLVGRVVQGLGAGGQLGLVNVTIADLIAVRERGMYLSYIGLTWAFASAIGPVLGGVFTEKVTWRLCFWINVPIAVLAMGSLICLLHLQSPKITVKEGLKRVDWLGIALVASGTVLSLLGLEFGGVSHPWHSPIVICFILFGMLILCSFVYVEWKVAKLPIMPIKLFTNRTNACAYFVGFFHGFIFIAGCYFIPLYFQAVRGDTAFMAGVYVLPYVMTLSGVSAISGLIIARTGRYQEVIWAGVAMTTLGAGLIVDLDRTSGWGKIVIYQIILGIGCGPLFQSPLIAIHANIDYRDVGTATVTFAFLRTMGTALTICIGLVVFQNSMQSQSRDLAGGRLPDKVLNAISGHSAASSVGFVQTLEPAERVVARDAYARGLSTMWCFFLAVSAGELYAARKGSEEEGWGGGGGGGVDLGSGYFSGYNLACIWVGNTIIECSRHDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJ23</accession>
    <name>D5GJ23_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nuclear protein localization protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00008787001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in the import of nuclear-targeted proteins into the nucleus and the export of poly(A) RNA out of the nucleus. Has a role in the endoplasmic reticulum-associated degradation (ERAD) pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NPL4 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840325.1">
        <property type="nucleotide sequence ID" value="XM_002840279.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJ23"/>
    <dbReference type="FunCoup" id="D5GJ23">
        <property type="interactions" value="911"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJ23"/>
    <dbReference type="GeneID" id="9181857"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008787001"/>
    <dbReference type="eggNOG" id="KOG2834">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017172_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJ23"/>
    <dbReference type="OMA" id="KWSRTGR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08061">
        <property type="entry name" value="MPN_NPL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016563">
        <property type="entry name" value="Npl4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007717">
        <property type="entry name" value="NPL4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024682">
        <property type="entry name" value="Npl4_Ub-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007716">
        <property type="entry name" value="NPL4_Zn-bd_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12710">
        <property type="entry name" value="NUCLEAR PROTEIN LOCALIZATION 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12710:SF0">
        <property type="entry name" value="NUCLEAR PROTEIN LOCALIZATION PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05021">
        <property type="entry name" value="NPL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11543">
        <property type="entry name" value="UN_NPL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05020">
        <property type="entry name" value="zf-NPL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010052">
        <property type="entry name" value="Polyub_prc_Npl4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0811">Translocation</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MPN" evidence="9">
        <location>
            <begin position="263"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="86"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="631"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="632"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ84516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="675" mass="74437" checksum="22FF49BE55123730" modified="2010-06-15" version="1">MILRFRSPDGAYRVDIQQTDDTSLLISKLRENLPKDVDPHTIIVSDRPANGRARVLNEISGMKISDLGLSHGDMLFLLYKKLNEPQAPNGAATSPGEPSSIRLNGQPIRPSESASNLYTTTTSGSVIPDKVSNPWETVVQDPVDEELDKKDGKIPRGRDHKMCRHGPKGMCDYCMPMEPYDPGYLAENKIKHLSYHSYLRKINTSTNKPELKSSYMPPLSEPYFRLRKNCPSGHQPWPAGLCSKCQPSAITLQPQEFRMVDHVEFSDPALINTFIDFWRQTGNQRIGYLYGRLAPYPEVPLGIKAIVEAIYEPPQNGESDGVTLQLPWTGEKDVAEVASLCGLRKIGVIFSDLLDDGTGQGTVVCKRHIDSYYLSSLEIVFAARLQAEHPHPSKWSETGQFGSSFVTCVVSGNEDNGIDVAAYQVSNTAVEMVRADIIEPSADPSVMMVHEEDGKTRYVPEVFFRHINEYGLNVKENAKPSFPVEYLLVTLTHGFPNDTKPIFTAPLGKFPIGNREIMGISQDHRAVAKQLGITSQSANRPTDGIDVVSDFHLLTFIKGWGVLDKNEEELLAKVAVGHDVNDGYKLLQTPGWQTLMTILRESGENAPLKRAFVEHSPPPPQIPLPFVNARGAGVGGSGSSNGGAARRGGRGDNATSSNNYGPLAKRFRGFSLREE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJ49</accession>
    <name>D5GJ49_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00008838001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840351.1">
        <property type="nucleotide sequence ID" value="XM_002840305.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJ49"/>
    <dbReference type="FunCoup" id="D5GJ49">
        <property type="interactions" value="93"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJ49"/>
    <dbReference type="GeneID" id="9181883"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008838001"/>
    <dbReference type="eggNOG" id="KOG1208">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_44_1_1"/>
    <dbReference type="InParanoid" id="D5GJ49"/>
    <dbReference type="OMA" id="NYLANYH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320">
        <property type="entry name" value="RETINOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320:SF285">
        <property type="entry name" value="RETINOL DEHYDROGENASE 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="277"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="368"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="377"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="445"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ84542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="483" mass="54899" checksum="9CBCA58C701BBDDD" modified="2010-06-15" version="1">MPIPILGEIYYNGIDSVPYLLPVLKFAPWILVVVVLKLYFNGYRNRNERVMHGKVAIITGGTSGVGAAVVNDLAARGAHLILLVRNVSDVFTVDYIEDLRERHGNPFIYAEECDLSSLHDIRLFATKFIDNTPPRRLDMVVCCAGVMAPPYTPRTTTKDGIEAHWGLNFLANFQLLNILAPCLRAQPPDRDVRILLATCSSYIAGDLDLNDPQFFRRQYPFGRPWLCYGASKMALMAFCLEFQRRLDSFVRPDKQPNNVRIYNIDPGLVRTPGARRWITMGSLWGLGVYLVMWPIWWLVLKDPQQGAQSFLAAAMSPECGMGEGGKFLRECQNQKYRAGELSSPELGRQLWELAELMIKDAEKAGAIKRAKEKGKKKAQEDMKDELKPDGEDKELMEKIRQRKEALRTVLEEEEAKQHEERAKAKLPPSLIPPPIYTPSRGTRSRTGELRTEEAKKVPMVKIETKEEVRDTPSRNTRRRTKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJ57</accession>
    <name>D5GJ57_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008847001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840359.1">
        <property type="nucleotide sequence ID" value="XM_002840313.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJ57"/>
    <dbReference type="GeneID" id="9184776"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008847001"/>
    <dbReference type="eggNOG" id="ENOG502RXHE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037541_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJ57"/>
    <dbReference type="OMA" id="RFPQDSY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018809">
        <property type="entry name" value="DUF2406"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28186">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 9 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28186:SF1">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 9 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10295">
        <property type="entry name" value="DUF2406"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="211"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="318"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="327" mass="35789" checksum="0C6A9DA6ECAAE47F" modified="2010-06-15" version="1">MTASVDPTRAITEEEPFQVNQSKQTSNLRATQFKDQYGNPIAEPDLSNPTRNRWERPLDTIRAFEDAIYKEHRRAPSYGDRRSGPSSETGNAADNQQQNHYDFHTRSNTSTPRNPHYDVYSGRSATPRSGMDGYGEPEAGYGRMNARSPIYNGRMSPRNHGHHQGYGYPQGPAGPPRPGHDRRQHYDPYAHGHRPNIAEGIGHPAHRNAPSPESYQSKPASNSSGPESDISHGSAGGPPAQPQPVDSYGFASGQGSGQPELFATSTQLYEPLSPGENWNAPKPNAPPTPPAEADQKAVPSPPPAQQPESNAVAEKRKSWFKRKMGKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJA6</accession>
    <name>D5GJA6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00008912001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJA6"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008912001"/>
    <dbReference type="HOGENOM" id="CLU_3428570_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJA6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ84599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="20" mass="2353" checksum="800123A2D21E0AD0" modified="2010-06-15" version="1">MIEMDALKYSATTMLQTKNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJF2</accession>
    <name>D5GJF2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008970001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430330">
        <property type="protein sequence ID" value="CAZ84645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840454.1">
        <property type="nucleotide sequence ID" value="XM_002840408.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJF2"/>
    <dbReference type="STRING" id="656061.D5GJF2"/>
    <dbReference type="GeneID" id="9182646"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008970001"/>
    <dbReference type="eggNOG" id="ENOG502QQCH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020809_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJF2"/>
    <dbReference type="OMA" id="VCDFYVQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021829">
        <property type="entry name" value="DUF3419"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47473">
        <property type="entry name" value="BTA1P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47473:SF1">
        <property type="entry name" value="METHYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11899">
        <property type="entry name" value="DUF3419"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13489">
        <property type="entry name" value="Methyltransf_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="809" mass="92241" checksum="4BC608B116CCB12D" modified="2010-06-15" version="1">MDSHPLILREPPNGNGRMFTPLDIQMFTLGAFLFAISLVVYFLLHAGKPYAVFFYNCFLKPFSGSSAEGNGGQQDALESFYKGQADIYDATRGKLLRGREEMLALAAAQLKYRVKKEGRKAVWVDIGGGTGFVSYFLSGKSIGLIGVRYNIETLNKYLSIQGFFSKVYLVDLSPSLCDVARERFGKLGWRNVEVVCMDARVFRLEEKADWISMSYSLSMIPDFYSVIDSLLSLLSSEGIISVADFYVQSLTNFAGRTYTGGVLSRHVNWFSRQFWRVWFEFDRVNLDEGRRDYLEYKFGTILSVNERNRILGGIPYYIWIGCQKLHTRTDVLALVDSATTKPPCLPPIHHMSGAQANAQLSDTMLKLRSKGFEAAVLNLATNLPLPSAFYQNRHWRIYYDELLQKHTQFNNSYIYAFTWEDPRVDQSILKIGPDDVVLAITSAGDNILHYLIEGSPKRVHAVDLNPTQNHLLELKLAAFSALPYEDIWNIFGLGRHENFGGLLVNKLSPHLSSRAFQYWFDHRQVFGSRLSGGLYETGQSRLAIIAAKWLFRVFGVSKHVERMCAARTLNEQREIWRSKVRPVILSWWVNWAIIDRDRFLWRALGVPANQRNMVIHDCPQLARGRDKDSGRAMWEYVVNTLDPVAENSLLSEDNYFYYLCLRGQYTEKSRPVYLCPRSHAKLSRPGALDNVRIHTDEINHVLSRIQPSTLSIAVIMDSMDWLETDGVEARKQIKSLNTAMMVGGRVLIRSAGRNPWYMRVLEEEGFKTARVGDRSPGACIDRVNMYASTWVCTKVEERGRMRGSSGIEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJJ5</accession>
    <name>D5GJJ5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">cystathionine gamma-synthase</fullName>
            <ecNumber evidence="10">2.5.1.48</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">O-succinylhomoserine (thiol)-lyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00009043001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the formation of L-cystathionine from O-succinyl-L-homoserine (OSHS) and L-cysteine, via a gamma-replacement reaction. In the absence of thiol, catalyzes gamma-elimination to form 2-oxobutanoate, succinate and ammonia.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>O-succinyl-L-homoserine + L-cysteine = L,L-cystathionine + succinate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20397"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:57661"/>
            <dbReference type="ChEBI" id="CHEBI:58161"/>
            <dbReference type="EC" id="2.5.1.48"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-cystathionine from O-succinyl-L-homoserine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the trans-sulfuration enzymes family. MET7 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.48" evidence="10"/>
    <dbReference type="EMBL" id="FN430332">
        <property type="protein sequence ID" value="CAZ84688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840497.1">
        <property type="nucleotide sequence ID" value="XM_002840451.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJJ5"/>
    <dbReference type="FunCoup" id="D5GJJ5">
        <property type="interactions" value="164"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJJ5"/>
    <dbReference type="GeneID" id="9182689"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009043001"/>
    <dbReference type="eggNOG" id="KOG0053">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011302_1_0_1"/>
    <dbReference type="InParanoid" id="D5GJJ5"/>
    <dbReference type="OMA" id="KVAKRCR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003962">
        <property type="term" value="F:cystathionine gamma-synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000111">
        <property type="entry name" value="Cystathionine gamma-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000063">
        <property type="entry name" value="Probable cystathionine gamma-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054542">
        <property type="entry name" value="Cys_met_metab_PP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051750">
        <property type="entry name" value="Trans-sulfuration_enzymes"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42699">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42699:SF1">
        <property type="entry name" value="CYSTATHIONINE GAMMA-SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00868">
        <property type="entry name" value="CYS_MET_METAB_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="4 12" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00051441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00058439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00060510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00066530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00083849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ84688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="542" mass="60462" checksum="90CDA0E75CDD51FE" modified="2010-06-15" version="1">MKTGYPRFFIHKSIQKLASTILSSFGQPGEEAMLFPTPSVAARCRQFIKATSRTTPVPSTRVVEFVSKIGKRNGGDGRLIRSDLCAVMYPASEAKVAKQFWQHTGDGVSSRRAEFCQREFDEGLLVEKSTISTESHPRMSKGPKRYSHRGMQFKPSAKEEEGLNMFVEERFGRNLDLEFVDSAKLAIRRRIAQSLEINMGLRDALGSSKDDGGMIEGFAENAVYLHPTGMSAIFNTHRLLMAILGPNKSVCYGFPYIDTLKILEKFGPGCHFYGHGSSEELGDLEQKLESGERILALFCEFPSNPLLKAPDLQRIKALADKYDFAIVIDETVGNFLNVHVLPYADVVVSSLTKIFSGDSNVMGGSSVLNPKGRYYKRLREALTTDYEDNHWCEDALFLERNSRDFVSRIERINRNAEAVADLLKSHPRIKQVYYPKYSPTKPFYDNCRCPGAGYGGLLSVTFYTAQDAIESFDALEICKGPSLGTNFTLASPYTLLAHYGELDWAAGYGVEDNLVRVSVGLEETGELLEKFRRALEALPSGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJK6</accession>
    <name>D5GJK6_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Eukaryotic translation initiation factor 3 subunit I</fullName>
            <shortName evidence="7">eIF3i</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Eukaryotic translation initiation factor 3 39 kDa subunit homolog</fullName>
            <shortName evidence="7">eIF-3 39 kDa subunit homolog</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">TIF34</name>
        <name evidence="9" type="ORF">GSTUM_00009054001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the WD repeat STRAP family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the eIF-3 subunit I family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430332">
        <property type="protein sequence ID" value="CAZ84699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840508.1">
        <property type="nucleotide sequence ID" value="XM_002840462.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJK6"/>
    <dbReference type="FunCoup" id="D5GJK6">
        <property type="interactions" value="1040"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJK6"/>
    <dbReference type="GeneID" id="9182699"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009054001"/>
    <dbReference type="eggNOG" id="KOG0643">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043845_0_1_1"/>
    <dbReference type="InParanoid" id="D5GJK6"/>
    <dbReference type="OMA" id="VWFSHNG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071540">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3e"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071541">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3m"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000127">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 subunit I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03008">
        <property type="entry name" value="eIF3i"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027525">
        <property type="entry name" value="eIF3i"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19877">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19877:SF1">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24805">
        <property type="entry name" value="EIF3I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 7" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="6"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="48"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ84699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="333" mass="37448" checksum="032914CD0EE59AD3" modified="2010-06-15" version="1">MRPILLQGHERSLTQIKYNKDGDLLFSTAKDHVVCAWYSVNGERVGTYKGHVGALWTVDVDPATTLLATGSADNTIRIWDVRTGKQLKMWEFPTAVKRVEFSEDGTLLLAVTEQRMGHLGTVVVFPITVDLEAEQVDEPNFTITFRESKATVAAWSYLNKYIITGHEDGRVSQYDGKTGELIRDVQAHELDMQITDIQMAPDRTYFITCSKDKTAKILDVNELKVMKTYVTDTPLNTAALTPKKDFVILGGGQEARDVTTTSQRQGKFEARFYHKIFEDEIGRVKGHFGPLNTIAVHPQGVGYASGGEDGYVRVHHFDKPYFDFAYEVEKMKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJM5</accession>
    <name>D5GJM5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00009076001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430332">
        <property type="protein sequence ID" value="CAZ84718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840527.1">
        <property type="nucleotide sequence ID" value="XM_002840481.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJM5"/>
    <dbReference type="STRING" id="656061.D5GJM5"/>
    <dbReference type="GeneID" id="9186752"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009076001"/>
    <dbReference type="eggNOG" id="ENOG502RZ6J">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_503527_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJM5"/>
    <dbReference type="OMA" id="TTSKCDN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030427">
        <property type="term" value="C:site of polarized growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005034">
        <property type="term" value="F:osmosensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030010">
        <property type="term" value="P:establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006972">
        <property type="term" value="P:hyperosmotic response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007232">
        <property type="term" value="P:osmosensory signaling pathway via Sho1 osmosensor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001402">
        <property type="term" value="P:signal transduction involved in filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039295">
        <property type="entry name" value="MSB2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35778">
        <property type="entry name" value="SIGNALING MUCIN HKR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35778:SF1">
        <property type="entry name" value="SIGNALING MUCIN HKR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="423"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="378"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="450"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="496"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="380"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ84718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="527" mass="53733" checksum="0B03350D69064D4F" modified="2010-06-15" version="1">MLPTDTSPPVSSPTTLPVDVPTFPVGTGGTAILPTGVTSHPTDTFIPGLPTSASLTPLPTDATATSFPITTPGPVSLPTNIGTTASGVTTPSVTPVPAPSNNDTTPSVATPTPTGTASLSVNATEPVPVGTGASPTSIISESKKHGTTVPLLTTPTTFTEKIGSATSLPVKPPTHVPTGAESVTTASGATTATMASTQWFPPELSTAPASTMITQVVTAIPGSTILSSATTTQSVETGIPSSFPKVVTPAGGMPEKPGNSSLVRVGFNHQLNYAFVVKNPVAVAQIFEYLPKGIAFGLGIKLSEVTMHHLQPLDTTKTKGYITTLAFAFIPTDMIDKLDMDRRNQNSRFFQSDDPPVREMINLLDPTISLRATEAELTGEGTGLPGEGSWLGGSGGNQGGASSNDGAPLGSNGSEGPVSKKSVGVGLGIVAGATVYGAAMFFVARRYRQRRNRHSRSSSIGRSVSPGSNPTSGLMSGVGPVMHGARSPYGAIGVPHDKRGSRGSGKASARTQNISGPMMAENSLGWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJM8</accession>
    <name>D5GJM8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Chitin synthase</fullName>
            <ecNumber evidence="5">2.4.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00009079001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Polymerizes chitin, a structural polymer of the cell wall and septum, by transferring the sugar moiety of UDP-GlcNAc to the non-reducing end of the growing chitin polymer.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>[(1-&gt;4)-N-acetyl-beta-D-glucosaminyl](n) + UDP-N-acetyl-alpha-D-glucosamine = [(1-&gt;4)-N-acetyl-beta-D-glucosaminyl](n+1) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16637"/>
            <dbReference type="Rhea" id="RHEA-COMP:9593"/>
            <dbReference type="Rhea" id="RHEA-COMP:9595"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17029"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="EC" id="2.4.1.16"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the chitin synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.16" evidence="5"/>
    <dbReference type="EMBL" id="FN430332">
        <property type="protein sequence ID" value="CAZ84721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840530.1">
        <property type="nucleotide sequence ID" value="XM_002840484.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJM8"/>
    <dbReference type="FunCoup" id="D5GJM8">
        <property type="interactions" value="73"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJM8"/>
    <dbReference type="GeneID" id="9186755"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009079001"/>
    <dbReference type="eggNOG" id="KOG2571">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004760_3_2_1"/>
    <dbReference type="InParanoid" id="D5GJM8"/>
    <dbReference type="OMA" id="TTMRETE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030428">
        <property type="term" value="C:cell septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004100">
        <property type="term" value="F:chitin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006031">
        <property type="term" value="P:chitin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902404">
        <property type="term" value="P:mitotic actomyosin contractile ring contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04190">
        <property type="entry name" value="Chitin_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004835">
        <property type="entry name" value="Chitin_synth"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914">
        <property type="entry name" value="CHITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914:SF38">
        <property type="entry name" value="CHITIN SYNTHASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01644">
        <property type="entry name" value="Chitin_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="345"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="389"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="420"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="463"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="594"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="627"/>
            <end position="649"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU366040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ84721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="692" mass="78386" checksum="E085A9ABA4519183" modified="2010-06-15" version="1">MYNEDEMHFTRTMHGVMRNIAHFCSRSKSRVWGKDGWQKIVVCIVADGRKKVHPRVLDALAAMGVYQDGIAKNSVNGREVKAHVYEYTTQVSLDSDLKFKGAEKGIVPVQMIFCLKELNAKKLNSHRWFFNAFGATLNPNVCILLDVGTRPGHDSLYHLWKAFDTDSNVGGACGEIKAMKGKGWMGLLNPLVASQNFEYKMSNILDKPLESVFGYITVLPGALSAYRYHALQNDESGHGPLSQYFKGETLQGKDADVFTANMYLAEDRILCWELVAKRNERWVLKYVRSATGETDVPDAVPEFVSQRRRWLNGAFFAATFSLAHFKQIWVTDHTIIRKVLLHIEFVYQFFSLLFTFFSLANFYLTFYFIAGSLSDPEMDPFGKGWGGRIFTVLRFMCVLLICTQFILCMGNRPQGAKRMFMSSMIAYSVIMAYTTFCSFYIVIKSFSGGFGDQKTEIKLDDNIFTNLIVSTGSTIGVYFLMSFLYLDPWHMFTSALQYFMMLPSYICTLQVYAFCNTHDVSWGTKGDNTVSVDLGAAVGSKGDTVELEMPSEQLDIDSGYDDALRNLRDRLEVLKEPMTEIQIKEDYYRAVRTYVVVVWMVANAILAMIVTEVYGLSGIGNNFYLKILLWSVAALAVFRALGSSAYLVLRAIHSFVEGRVKLDLKTFTDTFQPPWKRKLRLAGVSQSNLGRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>D5GK51</accession>
    <name>D5GK51_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00009365001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430335">
        <property type="protein sequence ID" value="CAZ84894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840703.1">
        <property type="nucleotide sequence ID" value="XM_002840657.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GK51"/>
    <dbReference type="STRING" id="656061.D5GK51"/>
    <dbReference type="GeneID" id="9186931"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009365001"/>
    <dbReference type="HOGENOM" id="CLU_2470712_0_0_1"/>
    <dbReference type="InParanoid" id="D5GK51"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00406">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ84894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="88" mass="9634" checksum="E9607B44402A6B84" modified="2010-06-15" version="1">MAPSLEDVASLKDLVKKLQERIEKLELSITGDPGSGADAPVDSLRMILMGPPGAGKGTQAPKIKEKFCICHLACFLLRCKYSEGYYQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKJ2</accession>
    <name>D5GKJ2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00009589001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430339">
        <property type="protein sequence ID" value="CAZ85035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840844.1">
        <property type="nucleotide sequence ID" value="XM_002840798.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKJ2"/>
    <dbReference type="STRING" id="656061.D5GKJ2"/>
    <dbReference type="GeneID" id="9184696"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009589001"/>
    <dbReference type="eggNOG" id="KOG4300">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037990_6_1_1"/>
    <dbReference type="InParanoid" id="D5GKJ2"/>
    <dbReference type="OMA" id="CEHVVNP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052356">
        <property type="entry name" value="Thiol_S-MT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45036:SF1">
        <property type="entry name" value="METHYLTRANSFERASE LIKE 7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45036">
        <property type="entry name" value="METHYLTRANSFERASE LIKE 7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="Methyltransferase" evidence="2">
        <location>
            <begin position="73"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ85035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="245" mass="27354" checksum="4B49955416EB6871" modified="2010-06-15" version="1">MLARTINYLTPAYLILLSAFFGVKALLLHLRLSSTTATFRDVWFTLFWHYSGPRFFANDPTISRIASQCRGKVLDIGPGTGDAIRFLPHPPLITHIYGLEPNIHMHAQLQKAVDAEGLTEVYTILPFGAEDLDGLRGAGVEAGSVDTVLTIRALCSLDKRLLDRAVGGLFECLKPGGQWLVFEHVRNGKCSVSGLLQGFYQILWPRMLAGCNINRRTSEILLRAGRWDVNDLVDLKEEEHWASCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKJ7</accession>
    <name>D5GKJ7_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 3 subunit J</fullName>
            <shortName evidence="4">eIF3j</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Eukaryotic translation initiation factor 3 30 kDa subunit homolog</fullName>
            <shortName evidence="4">eIF-3 30 kDa subunit homolog</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">HCR1</name>
        <name evidence="6" type="ORF">GSTUM_00009595001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the eIF-3 subunit J family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430339">
        <property type="protein sequence ID" value="CAZ85040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840849.1">
        <property type="nucleotide sequence ID" value="XM_002840803.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKJ7"/>
    <dbReference type="FunCoup" id="D5GKJ7">
        <property type="interactions" value="98"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GKJ7"/>
    <dbReference type="GeneID" id="9184701"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009595001"/>
    <dbReference type="eggNOG" id="KOG4813">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087988_0_0_1"/>
    <dbReference type="InParanoid" id="D5GKJ7"/>
    <dbReference type="OMA" id="KPHYALW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.60">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03009">
        <property type="entry name" value="eIF3j"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023194">
        <property type="entry name" value="eIF3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013906">
        <property type="entry name" value="eIF3j"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21681">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21681:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08597">
        <property type="entry name" value="eIF3_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="135"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="229"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="61"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="86"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="137"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="232"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ85040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="283" mass="30761" checksum="2168072908860B8C" modified="2010-06-15" version="1">MAPPKKWSGEEESSSGEEGVGPIAPRVAIKKKWDDEDSEEKVLDSWDAEDSEEERKKARAKQAAAENAAAEAAANKKSKAQRIAERQAQNAAQREATAAARESSNMEDETPAERRVREEQMRIEGDLAHATDLFGTVGVGAGSGGTKGKPITTGDPEDPTKSIDLSSLPLFAPKGKDEFTRLREVLVPLLVANSKKAHYSLFLQEFVRQLGKDMSSEQVRKVASGLTTLANERQREEKAAEKGGKKTKAAKGKTTLATGKEADKFDTTDYLEPAGYDGFDDFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKR2</accession>
    <name>D5GKR2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00009715001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430340">
        <property type="protein sequence ID" value="CAZ85105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840914.1">
        <property type="nucleotide sequence ID" value="XM_002840868.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKR2"/>
    <dbReference type="GeneID" id="9183341"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009715001"/>
    <dbReference type="HOGENOM" id="CLU_624369_0_0_1"/>
    <dbReference type="InParanoid" id="D5GKR2"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="9"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="439" mass="47679" checksum="798D3E479F9E2364" modified="2010-06-15" version="1">MAQTLHSSLLSAVFAYLDTQTLFTASLTCKHWRATTFLSRSHSPQLLRPHLSMLRAHFGRETVPHRIPIPTVEKLFTQYVRETAPAWMYSRRTYLLPPSHGAVSVSCDGGNGVEGKMAVVVGRNKVYVYDITESPITQTHTIQISDKVSKLAVKGGYLALSTKRKLHLYALLSNGLSLRLYSVPIPAVEAIALGRAGKAEEPLVAITTKDKTAIIHPSYALWRRRNNFFLQGSGSVAGASTGQSGRWEDCPWILVYRVTGERLSFKSDGRGVLVGNSLLGVWDGEERNLARTWKGFGRRLVLRVEGDGAVCFGSLVGAVNKGTVTVQTGEKIKEVSGGKCYTGCSQGRAAYLAFSRAPGQISVAPIGEGKEQTISLNSTSEILSITGGKERIVVAMGDRIVTLNLWNPSPDSQLKIEKDGSVPSSQPGTNTKYDACVIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKV1</accession>
    <name>D5GKV1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00009780001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430341">
        <property type="protein sequence ID" value="CAZ85144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840953.1">
        <property type="nucleotide sequence ID" value="XM_002840907.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKV1"/>
    <dbReference type="STRING" id="656061.D5GKV1"/>
    <dbReference type="GeneID" id="9183380"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009780001"/>
    <dbReference type="eggNOG" id="KOG0265">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_57_2_1"/>
    <dbReference type="InParanoid" id="D5GKV1"/>
    <dbReference type="OMA" id="IWDIRPY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052234">
        <property type="entry name" value="U5_snRNP_Component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44006">
        <property type="entry name" value="U5 SMALL NUCLEAR RIBONUCLEOPROTEIN 40 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44006:SF1">
        <property type="entry name" value="U5 SMALL NUCLEAR RIBONUCLEOPROTEIN 40 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="62"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="105"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="147"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="196"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="231"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="281"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ85144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="360" mass="38494" checksum="ACBCBF0FF363D893" modified="2010-06-15" version="1">MTDKRKASGSLGSSQLVKRIRPDGAGGDGSSAVSIVNSSASGGALIQVVPRTSALQAPIMELTGHSKEVFAVRFDPTGQNIASGSFDRSILVWRTYGDCPNYLALPGHKGAVLDLHWSRDSQIIFSASADTLLATWDVSTGGRIRRYVGHEDVVNAMDVTRRGPELMASGSDDGSIGIWDPRQKECIDYLETNFPVTAVAISEAGNELFSGGIDNDIKVWDLRKRSVAYTMRGHQDTVTSLSVSPDGQSILSNAMDSTVRTWDIRAFAPSNRLINTYEGAPSGIEKNLIRASWSPTGDKVGAGGGDGSVAVWEAETRKLLYKLPGHRGTVNDMRFSPIAAEPIIVSASSDKNILLGELGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLM4</accession>
    <name>D5GLM4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00010271001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430349">
        <property type="protein sequence ID" value="CAZ85417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841226.1">
        <property type="nucleotide sequence ID" value="XM_002841180.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLM4"/>
    <dbReference type="STRING" id="656061.D5GLM4"/>
    <dbReference type="ESTHER" id="tubmm-d5glm4">
        <property type="family name" value="Steryl_acetyl_hydrolase"/>
    </dbReference>
    <dbReference type="GeneID" id="9182720"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010271001"/>
    <dbReference type="eggNOG" id="ENOG502RDZA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047269_1_0_1"/>
    <dbReference type="InParanoid" id="D5GLM4"/>
    <dbReference type="OMA" id="YLEFLMA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050300">
        <property type="entry name" value="GDXG_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019436">
        <property type="entry name" value="Say1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN C4A8.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081:SF2">
        <property type="entry name" value="ALPHA_BETA-HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10340">
        <property type="entry name" value="Say1_Mug180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="391" mass="44664" checksum="D93D945A45A81918" modified="2010-06-15" version="1">MLLGQVSWLDILVFVVFLTWRLLRDVGIYGTVICAIQALPFLCWRMPTDIIKERYLTKHNRQSAFTRRSTLFQALVVACMRYGFTYVPTRIGRVFFTKGVALPFLRFRMFRHGYIWHLRHRPLWREINLNKGAMKGIWIVEDDIKKPDVVVYYCHGGGFAMGTCYFYLEFLISWVSILKESGFSNPAIFAVEYTLVPDKRYPVQLQEIISGYNHVLSITGDPGKVVVSGDSAGGALILSLLLHVGNRCPQKKPSFAALISPWTHIVSKLNRDAPSDFLSVSRLHEYGRLYAGSEKLWNPMVSPGFCMDISWWRKAMPVRGVHFYFGSEEIFYESIKALAKRLSGVGSVVCREDENLHAWPFAVLFLGAHEKERGRGLRRISSDIAGTLLHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLU8</accession>
    <name>D5GLU8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Histidinol dehydrogenase</fullName>
            <shortName evidence="11">HDH</shortName>
            <ecNumber evidence="3 11">1.1.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">GSTUM_00010427001</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the sequential NAD-dependent oxidations of L-histidinol to L-histidinaldehyde and then to L-histidine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>L-histidinol + 2 NAD(+) + H2O = L-histidine + 2 NADH + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:20641"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="ChEBI" id="CHEBI:57699"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="15">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 9/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11 16">Belongs to the histidinol dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.23" evidence="3 11"/>
    <dbReference type="EMBL" id="FN430351">
        <property type="protein sequence ID" value="CAZ85515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841324.1">
        <property type="nucleotide sequence ID" value="XM_002841278.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLU8"/>
    <dbReference type="FunCoup" id="D5GLU8">
        <property type="interactions" value="318"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GLU8"/>
    <dbReference type="GeneID" id="9186499"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010427001"/>
    <dbReference type="eggNOG" id="KOG2697">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006732_3_0_1"/>
    <dbReference type="InParanoid" id="D5GLU8"/>
    <dbReference type="OMA" id="YIAGPNH"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00014"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004399">
        <property type="term" value="F:histidinol dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06572">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1300:FF:000001">
        <property type="entry name" value="Histidine biosynthesis trifunctional protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1980:FF:000050">
        <property type="entry name" value="Histidine biosynthesis trifunctional protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1980:FF:000001">
        <property type="entry name" value="Histidinol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1980">
        <property type="entry name" value="Nitrogenase molybdenum iron protein domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01024">
        <property type="entry name" value="HisD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001692">
        <property type="entry name" value="Histidinol_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022695">
        <property type="entry name" value="Histidinol_DH_monofunct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012131">
        <property type="entry name" value="Hstdl_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00069">
        <property type="entry name" value="hisD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21256:SF2">
        <property type="entry name" value="HISTIDINE BIOSYNTHESIS TRIFUNCTIONAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21256">
        <property type="entry name" value="HISTIDINOL DEHYDROGENASE HDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00815">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000099">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00083">
        <property type="entry name" value="HOLDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00611">
        <property type="entry name" value="HISOL_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9 11" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="5 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 11 13" id="KW-0520">NAD</keyword>
    <keyword evidence="7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="330"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="331"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="364"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="364"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="418"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="423"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="423"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR000099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000099-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000099-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000099-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000099-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU004175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAZ85515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="438" mass="46925" checksum="170FD464894BC1AA" modified="2010-06-15" version="1">MARFNLKTSTPKELKEVLQRPAQKASDIMGIVTPIIESVKQRGDVALIEYAEKFDKAVGLKSAVLSAPFPPELMQLPQETIDAIDLSFENIRKFHEGQKEAGTLVVETMPGVRCSRFVRPIERVGLYIPGGTAVLPSTALMLGVPALVAGCKKIIFTSPPRKDGSLTPEIVYVAHKVGAEAIVLAGGAQAVAALAYGTETVPKVDKILGPGNQFVTAGKMFVSNDTGAGVSIDMPAGPSEVLVVADAEANPAFVASDLLSQAEHGVDSQVILITVGLTEEQLVAIEEELEKQALALPRVDIVRGSISHSVTLQTDNIEEAIELSNQYAPEHLILQIKDAAKAVDLVQHAGSVFIGHWTPESVGDYSAGVNHSLPTYGYARQYSGVNLSTYVKHLTSSELTEQGLRNVGGAVMQLAKVEELEAHRRAVGIRLEWLDSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLW4</accession>
    <name>D5GLW4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase</fullName>
            <ecNumber evidence="5">2.7.8.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">GlcNAc-1-P transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">N-acetylglucosamine-1-phosphate transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">GSTUM_00010445001</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. Catalyzes the initial step of dolichol-linked oligosaccharide biosynthesis, transfering GlcNAc-1-P from cytosolic UDP-GlcNAc onto the carrier lipid dolichyl phosphate (P-dolichol), yielding GlcNAc-P-P-dolichol embedded in the cytoplasmic leaflet of the endoplasmic reticulum membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a di-trans,poly-cis-dolichyl phosphate + UDP-N-acetyl-alpha-D-glucosamine = an N-acetyl-alpha-D-glucosaminyl-diphospho-di-trans,poly-cis-dolichol + UMP</text>
            <dbReference type="Rhea" id="RHEA:13289"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19507"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:58427"/>
            <dbReference type="EC" id="2.7.8.15"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:13290"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyltransferase 4 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.15" evidence="5"/>
    <dbReference type="EMBL" id="FN430351">
        <property type="protein sequence ID" value="CAZ85531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841340.1">
        <property type="nucleotide sequence ID" value="XM_002841294.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLW4"/>
    <dbReference type="FunCoup" id="D5GLW4">
        <property type="interactions" value="472"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GLW4"/>
    <dbReference type="GeneID" id="9187311"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010445001"/>
    <dbReference type="eggNOG" id="KOG2788">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029942_1_0_1"/>
    <dbReference type="InParanoid" id="D5GLW4"/>
    <dbReference type="OMA" id="LPHFNAR"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043541">
        <property type="term" value="C:UDP-N-acetylglucosamine transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003975">
        <property type="term" value="F:UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06855">
        <property type="entry name" value="GT_GPT_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000715">
        <property type="entry name" value="Glycosyl_transferase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033895">
        <property type="entry name" value="GPT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10571">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--DOLICHYL-PHOSPHATE N-ACETYLGLUCOSAMINEPHOSPHOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10571:SF0">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--DOLICHYL-PHOSPHATE N-ACETYLGLUCOSAMINEPHOSPHOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00953">
        <property type="entry name" value="Glycos_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="78"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="168"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="212"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="238"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="269"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="299"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00029567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00033238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00044717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00045078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CAZ85531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="456" mass="51043" checksum="5210780D3A44E06D" modified="2010-06-15" version="1">MTQKITTGWFISLFTIVGACLGLIWTVFIGDGEPLIASLGFSGIAFSASYCLIVWLGDSFKRVGLKGKDISKADRRELPETMGAVCAVVYLMCMIVFLPFAFYEYFVILTSGGGNRDATIEGVETGRLLHRFPHNKLGEYLSAILSLQSMVILGVADDLFDIRWRHKLFLPVIAAIPMLMVYYVDFGVTNIVVPTSLQEYIGTALLDLSWGYYLYMAAIAIFCPNSINILAGINGIEVSQSIIIGSFIAINDLLYISVEGHPATDSHLFSLYFLLPFLGVSLALWWHNWYPSKVFVGDTYCYFAGMTFAVVGILGHFSKTLLLLFVPQIFNFIYSAPQLFRLVPCPRHRLPKFNARSGLMEPSRAPFEKPPKWYIALILGILEKLRLIRVWRNKEGVIMECSNLTLINLWIVTFGPMREDKATMGLMGLQIACSIIGLLIRHKAALLVFPHDNLSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMA2</accession>
    <name>D5GMA2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="11">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00010620001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the universal ribosomal protein uS4 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430354">
        <property type="protein sequence ID" value="CAZ85645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841454.1">
        <property type="nucleotide sequence ID" value="XM_002841408.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMA2"/>
    <dbReference type="FunCoup" id="D5GMA2">
        <property type="interactions" value="1005"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GMA2"/>
    <dbReference type="GeneID" id="9183224"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010620001"/>
    <dbReference type="eggNOG" id="KOG3301">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089738_0_0_1"/>
    <dbReference type="InParanoid" id="D5GMA2"/>
    <dbReference type="OMA" id="RQFITHG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.10:FF:000021">
        <property type="entry name" value="40S ribosomal protein S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022801">
        <property type="entry name" value="Ribosomal_uS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018079">
        <property type="entry name" value="Ribosomal_uS4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005710">
        <property type="entry name" value="Ribosomal_uS4_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001912">
        <property type="entry name" value="Ribosomal_uS4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003139">
        <property type="entry name" value="PRK04051.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01018">
        <property type="entry name" value="uS4_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831">
        <property type="entry name" value="30S 40S RIBOSOMAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831:SF5">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00163">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01390">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00632">
        <property type="entry name" value="RIBOSOMAL_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS4 N-terminal" evidence="10">
        <location>
            <begin position="4"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="RNA-binding S4" evidence="9">
        <location>
            <begin position="109"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="163"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ85645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="195" mass="22617" checksum="0BC7A6C6E94454FD" modified="2010-06-15" version="1">MAPRSYSKTYNKSPCVPRRPFESARLDSELKIVGEYGLRNKREVWRVQLTLSKIRRAARQLLTLDEKDPKRLFEGNALIRRLVRVGVLDDSRMKLDYVLALRTEDFLERRLQTQVYKLGLAKSIHHARVLIRQRHIRVGKQIVNVPSFIVRLDSQKHIDFALSSPFGGGRPGRNRRKKAKAAESKGDGEEEEEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMP4</accession>
    <name>D5GMP4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00010865001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430358">
        <property type="protein sequence ID" value="CAZ85787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841596.1">
        <property type="nucleotide sequence ID" value="XM_002841550.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMP4"/>
    <dbReference type="GeneID" id="9185123"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010865001"/>
    <dbReference type="HOGENOM" id="CLU_2293754_0_0_1"/>
    <dbReference type="InParanoid" id="D5GMP4"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ85787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="101" mass="11370" checksum="D8692067E7AFE50F" modified="2010-06-15" version="1">MISYLDFLECIILRGLNNPVGWAPYGNGLRRYLVTRYGLVKPPCQSKPFNGRCKSGTEAADRASYVLGGAEYRGWALSMCYNHPECAASILTWWEFLISLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMS0</accession>
    <name>D5GMS0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein HIR</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">GSTUM_00010906001</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 12">Required for replication-independent chromatin assembly and for the periodic repression of histone gene transcription during the cell cycle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the WD repeat HIR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430359">
        <property type="protein sequence ID" value="CAZ85813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841622.1">
        <property type="nucleotide sequence ID" value="XM_002841576.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMS0"/>
    <dbReference type="FunCoup" id="D5GMS0">
        <property type="interactions" value="154"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GMS0"/>
    <dbReference type="GeneID" id="9185149"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010906001"/>
    <dbReference type="eggNOG" id="KOG0973">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004372_3_0_1"/>
    <dbReference type="InParanoid" id="D5GMS0"/>
    <dbReference type="OMA" id="RGSWDGD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000417">
        <property type="term" value="C:HIR complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000290">
        <property type="entry name" value="Protein HIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055410">
        <property type="entry name" value="Beta-prop_CAF1B_HIR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031120">
        <property type="entry name" value="HIR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011494">
        <property type="entry name" value="HIRA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019015">
        <property type="entry name" value="HIRA_B_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13831">
        <property type="entry name" value="MEMBER OF THE HIR1 FAMILY OF WD-REPEAT PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13831:SF0">
        <property type="entry name" value="PROTEIN HIRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24105">
        <property type="entry name" value="Beta-prop_CAF1B_HIR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07569">
        <property type="entry name" value="Hira"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09453">
        <property type="entry name" value="HIRA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="10 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12" id="KW-0678">Repressor</keyword>
    <keyword evidence="9 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="8 12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 11 12" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="20"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="67"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="CAF1B/HIR1 beta-propeller" evidence="15">
        <location>
            <begin position="105"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="138"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Protein HIRA-like C-terminal" evidence="14">
        <location>
            <begin position="679"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="400"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="594"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="800"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="407"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="601"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="804"/>
            <end position="813"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU364014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF07569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF24105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAZ85813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="928" mass="100849" checksum="4FFDBC035BC868ED" modified="2010-06-15" version="1">MHFIRPQWLTHSAEKKACEVYSCHVSPDGERLATGGLDGNVRIWSTRAVYGAADESASRLPKQLCSLSHHSGAVLTVRFSGNNRYLASGSDDKIVLVYERDLSADVGWSADSSVLVSVGLDSKVIVWSGSTFEKLKRIDVHQSHVKGLTFDPANKYFATASDDRSIKIFRFNSPAANATAHDQQTNFTVETSITTPFKESPLTTYFRRCSWSPDGSHIAAANAVNGPVSSVAIINRGNWDSEINLIGHEGPVEVCAFAPRMFSKTPLTPGTPPSTPVTVIACAGQDKALSIWNTSNPRPLIIAQDLAIKAISDLTWSPDGRTLFVCSLDGSIICICFEDGDLGFVLGLEENERILQKFGAGRKGATLPEGTESVRLEEMARDGERREVEGRMGELMMDGNGQAGDTPMSNSGSTAGPQWQMQQVPEAEKATNAPEQKEKPYKQKVTLTKDGKKRVAPLLVSTGGGHRSNLPNAHLLQASANNNSTISNDPKAILDLSRPYDGLPKGGMPALIFGNKRKAPVLEEGEEENPQQITNGKRIAVLASGGSGKQGPEETPEFIRPAVINPASSISQIRLGVPKVRNIVSRVVDSNGLPSSNVPNAAGGTSAAGAQASSSSDDYTFEARNLRNAGEPTRLTLSRHGQILWMDFVPKVALLVTGNSNFWSVACEDGTVHVFTPVGRRLLSPLILEAQPCFMECRGWWLMCITSIGMAYVWNLQTMKAPHPPVSLAPILDSSNTYQKQDSPTKAEAITEAGVNSEGTLIVSLTNGDGFAYSSDMYTWHKLTEAWWAIGSQYWDSTGAMRPAQNSSDNPTDPKSPAISAGVIPHLERRTTNEVLLHGRGRFLQRFETAVSLSHLENRMAAAVLLNAKDDFKNYLMMYARRIAAEGMKAKVEELLRDMMGKHRDLQRIAVPYARLLGVMDEDDSEDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMT6</accession>
    <name>D5GMT6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00010924001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430359">
        <property type="protein sequence ID" value="CAZ85829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841638.1">
        <property type="nucleotide sequence ID" value="XM_002841592.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMT6"/>
    <dbReference type="GeneID" id="9185165"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010924001"/>
    <dbReference type="HOGENOM" id="CLU_1688015_0_0_1"/>
    <dbReference type="InParanoid" id="D5GMT6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="95"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="156" mass="16665" checksum="A7B61E1F0D4DEE9B" modified="2010-06-15" version="1">MYLSTVMTAQIPDVKPTLFGLSSPRSPTNGYTCAFPSWPSRPCLDRRMPWEDDDDDEEEVSHECAGRKDGRCTCPTAVATAAPSSSAAERWDGRSVSSTCASSTSSTSAHATGGKVETLITGFEKLPLQPRSPRAPSRPTKSLLSLAILRKAGGDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMZ0</accession>
    <name>D5GMZ0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00011035001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430361">
        <property type="protein sequence ID" value="CAZ85903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841712.1">
        <property type="nucleotide sequence ID" value="XM_002841666.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMZ0"/>
    <dbReference type="GeneID" id="9183471"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011035001"/>
    <dbReference type="HOGENOM" id="CLU_467841_0_0_1"/>
    <dbReference type="InParanoid" id="D5GMZ0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056002">
        <property type="entry name" value="DUF7580"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24476">
        <property type="entry name" value="DUF7580"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7580" evidence="2">
        <location>
            <begin position="399"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="246"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ85903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="583" mass="64993" checksum="E89AF81CA0E0BBC3" modified="2010-06-15" version="1">MSTISLAVSVLQSFNRVETFLDPYQTLDRTAKKLKLRILTAKTLFRSEIKFLPGSSAWGADFPQGIRGSSGATKSTRGRQKVSENTSRDLVLRIDILPYILRIPILGYFLPAAGTRNERLLEGDIKIKLGSSYGRLRNSMRNAQTALENITEILEERARDAKSKSETILIFRSCLWGLMAILRVLSLGYPEVPVQTNIVPEKPVFERLGLGRCPGGSTGRLEELLAILEKEISIISEVRQLVIEPSGSESDPLQTSTVSSLPTPGEPANSEQLKSKQNHVINDLYNLLSRELGCRCHFTHLSLCSVSADPSSIKPMDSICSFFVTLDLNTCMDAYAARHPLGLTASRSLTPTLTPPRTENPTGCSTIITSPVGSQHLLVYQSPTRIEFLLKSTCPSLGSPNVNARSNSPPILTLRELLRSHSRGETSMKLGDRDRLLLAAKLAQWALQYNDTHLLYDLDARKIRFFTRYESDLNSGSWTPYLSTALNGPSPAHNERGGLYALGLVLLELGLQGPFIDESIDESRSEAAIRIALRKLLTKFGLGLRYRCIVKELLERRSREEKLINLEHWIRSIEEKDLKYLTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5GN41</accession>
    <name>D5GN41_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00011077001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430362">
        <property type="protein sequence ID" value="CAZ85934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841743.1">
        <property type="nucleotide sequence ID" value="XM_002841697.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GN41"/>
    <dbReference type="FunCoup" id="D5GN41">
        <property type="interactions" value="44"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GN41"/>
    <dbReference type="GeneID" id="9185860"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011077001"/>
    <dbReference type="eggNOG" id="KOG3798">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020884_2_0_1"/>
    <dbReference type="InParanoid" id="D5GN41"/>
    <dbReference type="OMA" id="LPCQYES"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070290">
        <property type="term" value="F:N-acylphosphatidylethanolamine-specific phospholipase D activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070291">
        <property type="term" value="P:N-acylethanolamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070292">
        <property type="term" value="P:N-acylphosphatidylethanolamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.15.10:FF:000048">
        <property type="entry name" value="Zn-dependent hydrolase/oxidoreductase family protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024884">
        <property type="entry name" value="NAPE-PLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15032">
        <property type="entry name" value="N-ACYL-PHOSPHATIDYLETHANOLAMINE-HYDROLYZING PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15032:SF4">
        <property type="entry name" value="N-ACYL-PHOSPHATIDYLETHANOLAMINE-HYDROLYZING PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038896">
        <property type="entry name" value="NAPE-PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="3">
        <location>
            <begin position="178"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="76"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="278"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="87"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="278"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>an N-acyl-1,2-diacyl-sn-glycero-3-phosphoethanolamine</name>
            <dbReference type="ChEBI" id="CHEBI:62537"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="414"/>
        </location>
        <ligand>
            <name>an N-acyl-1,2-diacyl-sn-glycero-3-phosphoethanolamine</name>
            <dbReference type="ChEBI" id="CHEBI:62537"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR038896-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ85934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="480" mass="53090" checksum="B3EF4DEA8E75708B" modified="2010-06-15" version="1">MRPRPASDLFGFLQSSHSCLLQSYRFVPRSLFSHNQIYSNNVIPHFSTVSSRTTTPTRMSSTRAVVASVYSVSVSTPQNLSSSPEDVANKSHHLENGKGFTNPWPSWRDLDPIKALKTIARHRVGPDSAGIWGPSTTPPTVPVVTPQFLPARSTVSTEFRATWLGHACFYVEFPGGFRVLFDPVFSERCSPVQFIGPKRYTKMPCDIADIPVVDAVVISHNHYDHLDHNTVIKLKKHHPDAWYFVPLGNKAWFACSGVEKCIELDWWEERDIVLTPSNSQISAGSSPSTDPNELSSKVQAEPTMEGPKANGIVARIGCLPCQHTSNRGLTDRGATLWASWSIESGGKKVYFGGDTGYRAVPPDLPKSVDDYGEEFRDLPVCPAFKQIGELRGPFDVGLIPIGAYVPRGVMSPMHANPYDAVRIFVDTKCNKALGIHWGTFVLTDEPVLEPPRLLKEALGKEGIEKEGVFDVLNIGETREY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GN44</accession>
    <name>D5GN44_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00011080001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430362">
        <property type="protein sequence ID" value="CAZ85937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841746.1">
        <property type="nucleotide sequence ID" value="XM_002841700.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GN44"/>
    <dbReference type="STRING" id="656061.D5GN44"/>
    <dbReference type="GeneID" id="9185863"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011080001"/>
    <dbReference type="eggNOG" id="KOG1840">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_454312_0_0_1"/>
    <dbReference type="InParanoid" id="D5GN44"/>
    <dbReference type="OMA" id="IQGVKHR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053137">
        <property type="entry name" value="NLR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46082:SF6">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46082">
        <property type="entry name" value="ATP/GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13374">
        <property type="entry name" value="TPR_10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="351"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="601" mass="67502" checksum="6DCA08D0E6B28B77" modified="2010-06-15" version="1">MFQRALAVGKEILGLGNQDTLLRVNNSIQLGRKRKWNDEAEVAYRHVSAGREKTRGSDHADTLRNGKSLSVVPHDQEEHDEAEIMYRRALVGREKILGPDHLDTLRAIYSLGTTLLRQGKDGEAEIMYRRALAGREKTLGRDHPSTLLNIHLLGVALYRQGKFEEAEIMQRRALAGREEAFGPDHPDTLSTISSLGRRKYEAGATYRHASGDTWKALGPDHPFSLESMNAVGETLLDLRKYNEAEIIFRLTLLGREKALGANHPATLTSTDNLGRTLHYQGNYKEAETMFRSAHLRREKAFGPYDLDTILSAHGLAVTLSDQQNYKEAEVMYRHALGGMEKSLGLDHPFTLESISTLGETLLDLKKYDEAEDMFRRAVADREKALGLAHRDTLLTVNNLGVALCNQRKHHEAEVMYRRALAGAEETLGPDHRDTLMCAYNLARALHKQRKYHEAEMMHRHALAGRERVLGPGHAYTLRSTINLGAVLCDQREYDTAEIMYRRAVTEGEKALGPSHWMTLKSANCLAVLAWALRGQKKCAGAEDICRRTLAGAEKALGPDHPDTLWNMNNLSVVLYSQGKHYKAEIIYRHALDRSSYPTECG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5GN56</accession>
    <name>D5GN56_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00011098001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430362">
        <property type="protein sequence ID" value="CAZ85949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841758.1">
        <property type="nucleotide sequence ID" value="XM_002841712.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GN56"/>
    <dbReference type="STRING" id="656061.D5GN56"/>
    <dbReference type="GeneID" id="9185875"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011098001"/>
    <dbReference type="eggNOG" id="KOG0417">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030988_13_0_1"/>
    <dbReference type="InParanoid" id="D5GN56"/>
    <dbReference type="OMA" id="WRAVMKG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23812">
        <property type="entry name" value="UBCc_ScPEX4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="2">
        <location>
            <begin position="10"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ85949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="166" mass="18040" checksum="3DAEBCBA6B96BEE2" modified="2010-06-15" version="1">MASSSSSPLSPTTRIMNELANYRKEPNPALESLGPVSEDDLFHWTTVLKGAEGTAYEGGRWKIDIKIPENYPHKAPEMKFLTSICHPNVHLKTGEICLDLLTNNWSAVCSISATLTSVQQLLASPEWDSPLNVDAAAVLRAGDTVGYESLVRVWSVLHAGKPPTLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5GND7</accession>
    <name>D5GND7_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase</fullName>
            <ecNumber evidence="1">3.1.3.67</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00011237001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.3.67" evidence="1"/>
    <dbReference type="EMBL" id="FN430363">
        <property type="protein sequence ID" value="CAZ86030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841839.1">
        <property type="nucleotide sequence ID" value="XM_002841793.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GND7"/>
    <dbReference type="FunCoup" id="D5GND7">
        <property type="interactions" value="38"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GND7"/>
    <dbReference type="GeneID" id="9182230"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011237001"/>
    <dbReference type="eggNOG" id="KOG2283">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020105_4_1_1"/>
    <dbReference type="InParanoid" id="D5GND7"/>
    <dbReference type="OMA" id="YIESWYR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016314">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043491">
        <property type="term" value="P:phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051896">
        <property type="term" value="P:regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051281">
        <property type="entry name" value="Dual-spec_lipid-protein_phosph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057023">
        <property type="entry name" value="PTP-SAK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029023">
        <property type="entry name" value="Tensin_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12305">
        <property type="entry name" value="PHOSPHATASE WITH HOMOLOGY TO TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12305:SF81">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3,4,5-TRISPHOSPHATE 3-PHOSPHATASE AND DUAL-SPECIFICITY PROTEIN PHOSPHATASE PTEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22784">
        <property type="entry name" value="PTP-SAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51181">
        <property type="entry name" value="PPASE_TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphatase tensin-type" evidence="5">
        <location>
            <begin position="1"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="4">
        <location>
            <begin position="66"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="309"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="322"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ86030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="369" mass="41591" checksum="C1E9869FA12EF89D" modified="2010-06-15" version="1">MSVPAAEFPKKAYRNPLDQVVKFLDSKHGQGWRIWEFRAEGTGYKDEDVFGRIFHAPFPDHHPPPFALVPAVLASMRNHLKGEGRENAVAVIHCKAGKGRSGTMSCAYLIGEEGWSADDALKHYTTVRMRPGFGEGVSIPSQRRWTRYIERWARELNKQYLERTIRIEEVHIHGLREGVRVAIQGFVDEGKEIKTYHTFTRGEQITLDSNETSSSRNAILKPKHPVILPANDINIDFEKRNIAAYGWSMVTSIAHVWFNAYFEGGEDSGLFEIEWEAMDGIKGTLRKGMRAFDRLKVVWSVDRSTEHIVREPSEGEPIPQPRRAETKLLHERNLGLKRKDDTASPASGSSGGSGVSLVNSTELLKAKDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNX9</accession>
    <name>D5GNX9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">acetyl-CoA C-acyltransferase</fullName>
            <ecNumber evidence="11">2.3.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">GSTUM_00011577001</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>an acyl-CoA + acetyl-CoA = a 3-oxoacyl-CoA + CoA</text>
            <dbReference type="Rhea" id="RHEA:21564"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:90726"/>
            <dbReference type="EC" id="2.3.1.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; fatty acid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.16" evidence="11"/>
    <dbReference type="EMBL" id="FN430371">
        <property type="protein sequence ID" value="CAZ86222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842031.1">
        <property type="nucleotide sequence ID" value="XM_002841985.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNX9"/>
    <dbReference type="FunCoup" id="D5GNX9">
        <property type="interactions" value="304"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GNX9"/>
    <dbReference type="GeneID" id="9183515"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011577001"/>
    <dbReference type="eggNOG" id="KOG1389">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031026_1_1_1"/>
    <dbReference type="InParanoid" id="D5GNX9"/>
    <dbReference type="OMA" id="MTAFPEP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003988">
        <property type="term" value="F:acetyl-CoA C-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010124">
        <property type="term" value="P:phenylacetate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00751">
        <property type="entry name" value="thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050215">
        <property type="entry name" value="Thiolase-like_sf_Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020610">
        <property type="entry name" value="Thiolase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020613">
        <property type="entry name" value="Thiolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01930">
        <property type="entry name" value="AcCoA-C-Actrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43853">
        <property type="entry name" value="3-KETOACYL-COA THIOLASE, PEROXISOMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43853:SF8">
        <property type="entry name" value="3-KETOACYL-COA THIOLASE, PEROXISOMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00737">
        <property type="entry name" value="THIOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00099">
        <property type="entry name" value="THIOLASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Thiolase N-terminal" evidence="15">
        <location>
            <begin position="33"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase C-terminal" evidence="16">
        <location>
            <begin position="296"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="13">
        <location>
            <position position="118"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="374"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000429-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAZ86222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="419" mass="43780" checksum="6B18EE1EB844E827" modified="2010-06-15" version="1">MSASARLNQITKQLAPGNNSARDNLLEKHADDVVVTCALRTALTKGGRGGFKDTPAQDLLVGVLKGLIERSKINPAVVEDVAVGSVLAPGGGATEFRAASLVAGFPVETAVYSLNRQCSSGLVACANIANAIRGGQIDVGVAAGVESMSTQYGPSAVTEFSELLDSNVQAKNCTVPMGVLSEQMAKDRNISRTRQDAFAAASFQKAEKAQKAGLFVEEIYPLEVSFEDPKTGETKRIIVDKDDGIRPGMTAEGLAKIRPAFAKDGSIHAGNASQISDGAAAVLFMRRSAAEKLGQKIIGKFVTSAVAGVEPLLMGIGPWASIPKVFAKTGLTKDDVDVFEINEAFASQCLWCAEELGLDKAKINPKGGAIAFGHPLGCTGARQVSTLMTELKRTGGRIGLTSMCIGTGMGMAAVWVAEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>D5GP65</accession>
    <name>D5GP65_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="14">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00011726001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430373">
        <property type="protein sequence ID" value="CAZ86330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842139.1">
        <property type="nucleotide sequence ID" value="XM_002842093.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5GP65">
        <property type="interactions" value="315"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GP65"/>
    <dbReference type="GeneID" id="9186570"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011726001"/>
    <dbReference type="eggNOG" id="KOG1246">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000991_0_0_1"/>
    <dbReference type="InParanoid" id="D5GP65"/>
    <dbReference type="OMA" id="GFDQVCK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034647">
        <property type="term" value="F:histone H3K4me/H3K4me2/H3K4me3 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="CDD" id="cd16100">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
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    <dbReference type="CDD" id="cd15518">
        <property type="entry name" value="PHD_Ecm5p_Lid2p_like"/>
        <property type="match status" value="1"/>
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    <dbReference type="CDD" id="cd15543">
        <property type="entry name" value="PHD_RSF1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PHD transcription factor (Rum1)"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PHD transcription factor (Rum1)"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PHD transcription factor (Rum1)"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="ARID DNA-binding domain"/>
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        <property type="entry name" value="Cupin"/>
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        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ARID_dom"/>
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        <property type="entry name" value="ARID_dom_sf"/>
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        <property type="entry name" value="JmjC_dom"/>
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        <property type="entry name" value="JmjN"/>
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    <dbReference type="InterPro" id="IPR013637">
        <property type="entry name" value="Lys_sp_deMease-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004198">
        <property type="entry name" value="Znf_C5HC2"/>
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    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
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    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
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        <property type="entry name" value="Znf_PHD-finger"/>
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    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
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    <dbReference type="PANTHER" id="PTHR10694">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694:SF33">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01388">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
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    <dbReference type="Pfam" id="PF08429">
        <property type="entry name" value="PLU-1"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF02928">
        <property type="entry name" value="zf-C5HC2"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="BRIGHT"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="ARID-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="2"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="JMJN"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="JmjN" evidence="12">
        <location>
            <begin position="89"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="ARID" evidence="11">
        <location>
            <begin position="154"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="10">
        <location>
            <begin position="469"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="domain" description="JmjC" evidence="13">
        <location>
            <begin position="611"/>
            <end position="777"/>
        </location>
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    <feature type="domain" description="PHD-type" evidence="10">
        <location>
            <begin position="1305"/>
            <end position="1354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="254"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="302"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="364"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1278"/>
            <end position="1299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1454"/>
            <end position="1645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="28"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="66"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="368"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="411"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1278"/>
            <end position="1291"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1522"/>
            <end position="1536"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1566"/>
            <end position="1575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1576"/>
            <end position="1595"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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            <dbReference type="ARBA" id="ARBA00022833"/>
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            <dbReference type="ARBA" id="ARBA00023004"/>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
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    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51011"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS51183"/>
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        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ86330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1697" mass="189125" checksum="4C97C72EEBC5957D" modified="2010-06-15" version="1">MPAAASSASSAAASSARPSPRAPSTRIKGGGGHGGGGGAPSNLPGPGGYQSAPLSSRKAQPLDMLTVERRSSSSKENPKRIRPHGLQEAPTYRPTAEQFKDPFEYIKSIAEEGKKYGIIKIIPPDTWNPEFAVDTERFHFRTRRQELNSVEGGTRANLNYLDQLSKYHKQHGTNLNRFPSVDKRPLDLYRLKKAVEIRGGFTNVCKHKKWAEIGRDLGYSGKIMSSLSTSLKNSYQRYLHPYEEWVKSAKPGVQQQIEAEQGGPITPSGSPLKKLPADKPAMSPLSSLPDSPAIRASAALNASVVGTPERSDTDMADATPIRGSTPAVNGGFTAVNTGSGFTPVNSGAGGFAIVNVSNGMKRELDANMTPNTGNSRRSENGATPSKRSTPELKAGTILKRGHSQENGSGADIKEQSKEATADESENGERRSKRLKKEPAPMVAGSHMTLHRPSSTPRTLGGDRNNSKPGEKCEKCGRGDDMANLLLCESCDHGYHTFCLEPVLKSVPDRDWYCDRCLVGTGDYGFADGEIYSLRQFQEKANNFKDLYFQSKMPFDPVLNKARQVTEDDVEREFWKLVESVHETVEVEYGADIHSTTHGSGFPTIEKQPTYPYATDPWNLNNLPLHPESLFRHIKSDVSGMTVPWLYVGMCFSTFCWHNEDHYTYSANYQHFGATKTWYGIPGSDAMKFEDAMREAVPELFEQQPDLLFQLVTLLTPQHLMKAGVKVYALDQRAGQFVVTFPQAYHAGFNHGFNFNEAVNFAPSDWEPFGQAGVERYLEFRKAPVFSHDELLLTAAARDTTIKTSQWLAPALARVRDRELQARRGLLEHLPDIKQATLPEDEELSEDQYQCGVCKVYCYLSQITCPCTPNVTCPSHFRDICDCEDTRLTLRLRMTDESLEELVQRVHDKANMPKSWAVKLEKSIAESPRPALKVLKSLLSEGERIPCYIPELAPLKAFVERAQEWVDEATPFISRKQQNRRKNEKAWRRMGSKVAEMEEKEREHRKLDSILKLLERAEDIGFECTEIEQLRERADLIRAFQLRARKALATPGTLSTPMYEELVETGKSFNVDLAETDLLEKIVRQLKWADSARDARARFLSLTEVAELIKSGQELGIPDTNEQMMHFRSQKEAGDMWEKKAKELMSVEIVHFQQLEALSGQASTLPVSRETLAQVDQILNKQREAHRQIVALYEKSKEADFYKRPKYKEVRDVLDGLTELHSKPTGTIDLEKEQKRHEDWMRRGKKLFGKANAPLHILAQHMAYVEGRNKHCFCLEDRPRTPVEPSSREPSPDSKSNGPFGEGPREVFCICRQPEAGMMIECEVCHEWYHGKCLKIARGKVKEDEKYTCPICDYRVKIPRDAARPKLEELIDWQAEIENLPFIPDELDCLHRIIDAATKFREWVRPFTTAIGLTGAEVPTMRFYLRKIEGAEILLSQETNFFRTELHRWMPIAPDPPPVVEVSMSTRKPRPTKQQKLMAQMGITNPDDLPPQLRTKAHSFSKKKPVEANGKPPPPIKPAPSKGSMTSSPVTPTSQTHPLLGQSSSSSSQPPPSQPQFDYPRFGSAGPSAQSPTFTLAHSSNSSSGNGASSSSSQHQRLGSPVLLNNPGRGPTLDPALFNPQAFPGDHSGHGHRGVGGGPGSPQYSSAKAMFDALTEDPDLYSKEETSGGVSYEALAAEALNTAGEALDEKLADQFLAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5GP67</accession>
    <name>D5GP67_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00011728001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430373">
        <property type="protein sequence ID" value="CAZ86332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002842095.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GP67"/>
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        <property type="interactions" value="1013"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GP67"/>
    <dbReference type="GeneID" id="9186572"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011728001"/>
    <dbReference type="eggNOG" id="KOG0264">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020445_3_1_1"/>
    <dbReference type="InParanoid" id="D5GP67"/>
    <dbReference type="OMA" id="PHEEGCL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022052">
        <property type="entry name" value="Histone-bd_RBBP4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050459">
        <property type="entry name" value="WD_repeat_RBAP46/RBAP48/MSI1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22850">
        <property type="entry name" value="WD40 REPEAT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12265">
        <property type="entry name" value="CAF1C_H4-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 6" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Histone-binding protein RBBP4-like N-terminal" evidence="7">
        <location>
            <begin position="30"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="186"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="282"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="326"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="383"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ86332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="436" mass="48869" checksum="6C7D126F9EBBF2BB" modified="2010-06-15" version="1">MSTNMDDDIDMDPSLRDHEEQIVVDKIINEEYKIWKKNAPFLYDLILSSALEWPTLTTQWFPDKQKHTGKNYSTHRLLIGTHTSGNDTNYLQIAEVQLPNPVTEHDPNRYDEDKEEIGGYGGGAECRLHIQQKMVHEGEVNKARYMPQKPDLIATMCADGNVLVFDKTKHPLMPTNTSKCTPQMTLVGHGKEGYGLNWNPHKEGRLMTGSEDSTVRLWDLNSYTKTNTTLHPIHTYTHHSAIVNDVAYHPCHDALFGSVSDDHTLQIVDTRSSDTTTAVHKVVAHADAVNSIAFNAASDYVVATASADKTVALWDLRNLKLKLHSLQGHNAEVNGLSWHPHEEPILASSSADRRIIFWDLARIGEEQSPEDAEDGPPELLFMHGGHTNRVSDFAWNPNDPWVMVSAAEDNLIQCWKVSSAIVGKDMEDVPVGELET</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPF8</accession>
    <name>D5GPF8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00011828001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430375">
        <property type="protein sequence ID" value="CAZ86401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842210.1">
        <property type="nucleotide sequence ID" value="XM_002842164.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPF8"/>
    <dbReference type="GeneID" id="9186688"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011828001"/>
    <dbReference type="HOGENOM" id="CLU_2869288_0_0_1"/>
    <dbReference type="InParanoid" id="D5GPF8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ86401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="64" mass="7300" checksum="E1B9A26F7C8EDD12" modified="2010-06-15" version="1">MRNNRQPRALPPHQEPVHRQEPNIHATRHPHVGVKPADHDPPCALQSLTHCRGRARGTKTRHEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPP4</accession>
    <name>D5GPP4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00011959001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430377">
        <property type="protein sequence ID" value="CAZ86487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842296.1">
        <property type="nucleotide sequence ID" value="XM_002842250.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPP4"/>
    <dbReference type="GeneID" id="9184307"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011959001"/>
    <dbReference type="eggNOG" id="ENOG502SA1F">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058677_2_0_1"/>
    <dbReference type="InParanoid" id="D5GPP4"/>
    <dbReference type="OMA" id="VTIMEPP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="158"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="85"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="162"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ86487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="216" mass="22778" checksum="A9A543A2597E6D8B" modified="2010-06-15" version="1">MSSARTSEQVTNGQPPSYPHARRGSLGPGTLTDFFSTSRVPTTSAYPGPITSAAAQANRRRLSMSNASGTSPPRVQTNMSMRRGSISSVSSASSAVDESAIEEGEVNAGSPSSLGSSPFARRLSFGARALRDVRIPQGRNPGNFPVSAGAASPTVTRGFWLDGRSGSQSQGSDRDEAPVQRRQSISVIPPPVNAISPKDAHDPLQERMLKGDFYMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPQ7</accession>
    <name>D5GPQ7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00011977001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <dbReference type="EMBL" id="FN430377">
        <property type="protein sequence ID" value="CAZ86500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842309.1">
        <property type="nucleotide sequence ID" value="XM_002842263.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPQ7"/>
    <dbReference type="FunCoup" id="D5GPQ7">
        <property type="interactions" value="118"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GPQ7"/>
    <dbReference type="GeneID" id="9186732"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011977001"/>
    <dbReference type="eggNOG" id="KOG0876">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057349_1_0_1"/>
    <dbReference type="InParanoid" id="D5GPQ7"/>
    <dbReference type="OMA" id="MTAREPN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.40.20">
        <property type="entry name" value="Iron/manganese superoxide dismutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019832">
        <property type="entry name" value="Mn/Fe_SOD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036324">
        <property type="entry name" value="Mn/Fe_SOD_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036314">
        <property type="entry name" value="SOD_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43595">
        <property type="entry name" value="37S RIBOSOMAL PROTEIN S26, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43595:SF2">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02777">
        <property type="entry name" value="Sod_Fe_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54719">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46609">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Manganese/iron superoxide dismutase C-terminal" evidence="2">
        <location>
            <begin position="125"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Manganese/iron superoxide dismutase C-terminal" evidence="2">
        <location>
            <begin position="215"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ86500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="273" mass="30732" checksum="453C755288410DDF" modified="2010-06-15" version="1">MSVPLTLRRQALRAFPPLTRKPPTLHKPRSVHSMPVISLTEKGVPGLFSEKAFKTAWTDYQKHLVEKLGSVTAGREFDGMDTMDIAIATARQPDQASIFNYASQAYNNHFFFQSLRSDQEEITAQFKAYVISTFVSVDALKAELFATANAMFGNGYVWLVLDQAGSLRILCTYNAGTPYGASYRRQDTDMNTGLRLGSELPPYTSAIRNAAKGKTGGWVFPVLNVNVWEHAWLEDFGILGKDNYLEAWWKHIDWMVVQDRFPKKGVARAPMNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPT0</accession>
    <name>D5GPT0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00012016001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430378">
        <property type="protein sequence ID" value="CAZ86523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842332.1">
        <property type="nucleotide sequence ID" value="XM_002842286.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPT0"/>
    <dbReference type="FunCoup" id="D5GPT0">
        <property type="interactions" value="16"/>
    </dbReference>
    <dbReference type="GeneID" id="9182267"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012016001"/>
    <dbReference type="eggNOG" id="ENOG502QU6M">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021993_3_1_1"/>
    <dbReference type="InParanoid" id="D5GPT0"/>
    <dbReference type="OMA" id="VEFMIEV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000286">
        <property type="entry name" value="MFS efflux transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051788">
        <property type="entry name" value="MFS_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23514">
        <property type="entry name" value="BYPASS OF STOP CODON PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23514:SF15">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_8G05090)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="101"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="128"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="157"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="194"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="222"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="270"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="337"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="361"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="395"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="424"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="70"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="26"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ86523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="451" mass="48696" checksum="0A05ECEB1DB44CCC" modified="2010-06-15" version="1">MTAEIAKIPPPVAPIELENRSLAAGSNFAPIGTPSRSISAGDEESSQLKDRPPEGVTIFERPNVSTMKGELLSMNFAVFISGMNDGSTGALIPYLRPAYDIGLLFVAVVYLINFSGWLFAAFTNVHLTARLGMGGVLTLGAALQLSAYCLMFWKPPYPLFVCSFFFSGLGIAYQNAQANVLVANLNNSHRWLSILHATFGVGALLGPLIATTIAARTAYWHYFYLVTLGLAVINLGLLAWMFRDGLFKGTPAAKESANRDFKKALSERTVIVLSLFFLLYVGMEISAGGWVVEFLIRARNGKPSKVGYVASGFWGGLTLGRVVLADVTHRLGERRMVFVYILLALIAQLIFWFVPNIIANAFMISLLGFFIGPFFPTGISVATKVLPRELHVASIGFAATMGQAGSAAFPFLTGAIAAKSGVRVLQPMLVALLAGMSIFWALIPRASRHIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5GQ25</accession>
    <name>D5GQ25_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00012164001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430382">
        <property type="protein sequence ID" value="CAZ86618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842427.1">
        <property type="nucleotide sequence ID" value="XM_002842381.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GQ25"/>
    <dbReference type="STRING" id="656061.D5GQ25"/>
    <dbReference type="GeneID" id="9185198"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012164001"/>
    <dbReference type="eggNOG" id="KOG1674">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_389426_0_0_1"/>
    <dbReference type="InParanoid" id="D5GQ25"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20557">
        <property type="entry name" value="CYCLIN_ScPCL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013922">
        <property type="entry name" value="Cyclin_PHO80-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615:SF36">
        <property type="entry name" value="PHO85 CYCLIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08613">
        <property type="entry name" value="Cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="495"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="602"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="661"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="519"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="602"/>
            <end position="614"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ86618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="709" mass="75978" checksum="9CC0032019220812" modified="2010-06-15" version="1">MTLAHTPPTATITHSRYSYSPAPSIASSASSSQSSGWSVASAVSNTTSVASCTGGASAPGTNIIPSLNIQRQFAGSEQDGWLLRKRVSVSSLRGSDSIQHPHPPSVTVAQEPVPIEQRRNARRTNRLVEGANAGCPVSEGCPLPPVPTLQRQVDRKVNFVDNLVDSAAQIVETIWPPPKMLICATPKDKVLPLRTFIQETLRRSRTSYSTLQVALYYLIVIRPHLPPRDCDISSLPAEEAQMTRAKQCGRRMFLAALILASKYLQDRNYSARAWSKISGLNTQEINVNEMAFLDAVNWRLHISETNFQKWTDLVLKYTSDHNPSSASSARANPWVDEEERKREWCDLVLKLSPDLNEAELLASGGLTPCAVDADIVASTSPLTIEPFVPIAPAQQTPAAPVAAQPVATLAPRATEHLQTSHPHAFKALHQPLPKAPAFRRSSSSQLHSGTVTPAAGICSSRLEVTSASRCSAMASVAKIAENALSGATMEKFPFAPRRGSDALPPPVLPPSQGGCIRRSSLSASVSKPGPSSSPAFPKAKQSCELSKLVFTTFAPPSPSSVASSVKNICTSPRKTGDHRMTPCVSPKSREAALSLNMLKETYSPCSSGSTTPTGLSRKRSYPESAMEGHPGSALRVEANVHGYAADGTTSVTPKFKSLNSGFRSPSLAYGSPTRSPAPDRKGKRLRCAPEKKMGGMNMIEGMSVAQNVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>D5GQ29</accession>
    <name>D5GQ29_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00012174001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="FN430382">
        <property type="protein sequence ID" value="CAZ86622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842431.1">
        <property type="nucleotide sequence ID" value="XM_002842385.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GQ29"/>
    <dbReference type="STRING" id="656061.D5GQ29"/>
    <dbReference type="GeneID" id="9185202"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012174001"/>
    <dbReference type="HOGENOM" id="CLU_714090_0_0_1"/>
    <dbReference type="InParanoid" id="D5GQ29"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.220">
        <property type="entry name" value="Fungal kinase associated-1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031850">
        <property type="entry name" value="Fungal_KA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043024">
        <property type="entry name" value="KA1_sf_fungal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16797">
        <property type="entry name" value="Fungal_KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Fungal kinase associated-1" evidence="9">
        <location>
            <begin position="265"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="22"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="52"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ86622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="387" mass="42589" checksum="9FBFB79CFAA54120" modified="2010-06-15" version="1">MPHIFEKEKIAKLEAQNQGLGITRGGVGSKSRVVSAPQPNRNVSLARRHPKRQSDNLTRVDKFEPAPLRIRPKSVVNRHVPIDRAGDGSRNSKRLSQLPAVKDESAGFNPELILPSTHGDERQSLRLANDETRTIATKVNPVMSGALATAVMEPLVKGVKKKRSLFDVLKGKSKIAPESKDPAVEEIVSGGIKGTFGRRSGNLLKRWASRDAGGLLAFGTSNDRGDTQSIGNGLTSCFTVPQGSKEGSGSFGGFEEDQFTKVMSQRNWFMKFLNVKPASRVICFNISPMVARKEIMKLWKEWVKYGLVIVEDDRKSLVMRARVGTPNSLNIKEVSFVGEIRAYGGSRKTGSGVVRFTQERGAASSFNRVIGEMTKKFSEKRIILPED</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>D5G3X1</accession>
    <name>D5G3X1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DNA replication licensing factor MCM5</fullName>
            <ecNumber evidence="12">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">GSTUM_00003837001</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the MCM2-7 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the MCM family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="12"/>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835093.1">
        <property type="nucleotide sequence ID" value="XM_002835047.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G3X1"/>
    <dbReference type="FunCoup" id="D5G3X1">
        <property type="interactions" value="932"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G3X1"/>
    <dbReference type="GeneID" id="9187152"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003837001"/>
    <dbReference type="eggNOG" id="KOG0481">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000995_7_2_1"/>
    <dbReference type="InParanoid" id="D5G3X1"/>
    <dbReference type="OMA" id="ITYCKTR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071162">
        <property type="term" value="C:CMG complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005656">
        <property type="term" value="C:nuclear pre-replicative complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006267">
        <property type="term" value="P:pre-replicative complex assembly involved in nuclear cell cycle DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006279">
        <property type="term" value="P:premeiotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030174">
        <property type="term" value="P:regulation of DNA-templated DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17756">
        <property type="entry name" value="MCM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000241">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.10">
        <property type="entry name" value="mini-chromosome maintenance (MCM) complex, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008048">
        <property type="entry name" value="MCM5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054125">
        <property type="entry name" value="MCM5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF42">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21933">
        <property type="entry name" value="MCM5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01661">
        <property type="entry name" value="MCMPROTEIN5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 12" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="14">
        <location>
            <begin position="320"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU368063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAZ79214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="720" mass="79123" checksum="FD79695D32C37F4D" modified="2010-06-15" version="1">MDRSSVFTIDTGLPKDDSQPDDQPGEVQRAFRSFILEFRLDNNFIYRDALRTNVLIKKYMLDVDMAHLISFNEELAHRLANEPAEVIPIFEIAIKECAKRMFFPGADSSRGFPECQLILHSTANMISIRDLNASYISKLVRIPGIVVGASTLSSKATVLSIMCRDCSNTKKIPVGGGFQGISLPRVCDRQQVQGEEAQKCSLDPFFVVHEGSTFIDQQVLKLQEAPDMVPVGELPRHILVSADRYLTNRVVPGSRCTVMGVFSIYQSKGGKGPAAAVAIRNPYIRVVGIQSDVDASAAGNASFTGEEEQEFLELSRNPNLYEMFANSIAPSIYGNADIKKAIACLLLGGTKKILPDGMKLRGDINVLLLGDPGTAKSQLLKFVEKVSPIAIYTSGKGSSAAGLTASVQRDNNTREFYLEGGAMVLADGGVVCIDEFDKMRDEDRVAIHEAMEQQTISIAKAGITTILNARTSVLAAANPIFGRYDDMKSPGENIDFQTTILSRFDMIFIVKDDHNPERDARMAKHIMGLHMNQLPQGEEETGDISIAKMKRYITYCKTRCAPRLSPEAAEKLSSHFVSIRKRVHQAEVDANERSSIPITVRQLEAIIRITESLAKLSLSPIATEDHVDEAIRLFLASTMDAVGQGAATSSELMEEINKVEAELRRRLPIGWSTSLATLKRELCVNKGYSEAALSRALVIMSRRETIQFRSGGGSVFRSGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>D5G459</accession>
    <name>D5G459_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00003961001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835181.1">
        <property type="nucleotide sequence ID" value="XM_002835135.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G459"/>
    <dbReference type="FunCoup" id="D5G459">
        <property type="interactions" value="379"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G459"/>
    <dbReference type="GeneID" id="9182429"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003961001"/>
    <dbReference type="eggNOG" id="KOG2793">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1826693_0_0_1"/>
    <dbReference type="InParanoid" id="D5G459"/>
    <dbReference type="OMA" id="EPLILNW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF152">
        <property type="entry name" value="PROTEIN-LYSINE N-METHYLTRANSFERASE EFM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10294">
        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ79302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="141" mass="15370" checksum="534102AB2CF52629" modified="2010-06-15" version="1">MDLFCDNSDSEVEPFGADLVEPAPILKPRIIETTFHGLLSSPIKLHEDLAKGCGGQIWPAGETLAKYLLRRYGRGNNGLRGKRIVELGAGGGLVGLAITAGCDIFGSELYITDMEAMMEMMARNVALNGFEDRVKVQLLDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4D8</accession>
    <name>D5G4D8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00004064001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835260.1">
        <property type="nucleotide sequence ID" value="XM_002835214.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4D8"/>
    <dbReference type="STRING" id="656061.D5G4D8"/>
    <dbReference type="GeneID" id="9185006"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004064001"/>
    <dbReference type="eggNOG" id="ENOG502SDF2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_567643_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4D8"/>
    <dbReference type="OMA" id="RIWGSER"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013860">
        <property type="entry name" value="AreA_GATA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08550">
        <property type="entry name" value="GATA_AreA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitrogen regulatory protein areA GATA-like" evidence="2">
        <location>
            <begin position="32"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="304"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="457"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="214"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="239"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="265"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="279"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="306"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ79381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="481" mass="52118" checksum="41DD0717B9356C14" modified="2010-06-15" version="1">MKPIGGIVTNKKSIPDNFRQVETFTTEYLSKLWKIYTVNRDVLDKGRRLENLFWRIWGSERIQRTFSGKTVSKIFLMILEGEGPFEKTRFRIPPLPIPTPPHPPHPESTPRPSLEIECPPTPPPSPMPPVTSIAGFQLGSSYLSKIPSEMAITASLHQAFPPRLLSPPPPSRTNPSMAEQSTDSAPPQNDPPGPSRLATPPNTKMIVGAAAAGSKPSSLVSSENESVSSKEGGCGKNPAVKERKARTERPPRGRRKVGVSTRVTATRRSRPAGPRRKSSSSSNTAVNTAFSSPLQTLAGDLARSLPSDSVRQQHKVSSTDGGDFESDAVGKRGDTADSNWIVDPDFRTKYLERKRKEKLVAWSSEAFVVPLVTKAVPTAAVVESQATASTKISRGKGKGVVLVDQIVPLKGLSEEATDLQATRVLVANVRDEGIDELPATLVRRKSELTLMFEAEAKRSGEKVGKGGPGKEEAITEGEMTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4K7</accession>
    <name>D5G4K7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00004200001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="CAZ79450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002835247.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="9188385"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004200001"/>
    <dbReference type="HOGENOM" id="CLU_345520_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4K7"/>
    <dbReference type="OMA" id="DVYAHER"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="156"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="261"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="180"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="378"/>
            <end position="398"/>
        </location>
    </feature>
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        <location>
            <begin position="413"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="517"/>
            <end position="526"/>
        </location>
    </feature>
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        <location>
            <begin position="552"/>
            <end position="563"/>
        </location>
    </feature>
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        <location>
            <begin position="674"/>
            <end position="683"/>
        </location>
    </feature>
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        </location>
    </feature>
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            <end position="744"/>
        </location>
    </feature>
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            <begin position="770"/>
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        </location>
    </feature>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ79450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="818" mass="88436" checksum="6C0242074688DD63" modified="2010-06-15" version="1">MFDQLFSRRNSEVEHTSPTRESGTPVSPHMPISQCIAGEVAGIGISIGYCDSPPPIISVESIVAAKPWYEDFPDYSLISVGPRDGNEEEHSSAGHTRGWNASGFLAAICGIGIALSFWTPRNLWVIMGGFGLRREKRQQKSREQVRVRAREDVYAHERIGTRSGGKGKQRAIEASESEGESSTTLGESTPLLVGPHVPSYNKIDVYDPDEEPKPTFNRGDLEPVPVSPVENLLTTIEEDIEPRSLPKNSGFQLPERKLQTEQPILEGPEAAEVATPDSEGQCPFHVRKNSLQIQPLPVSIVIPTGKPASIRRPRSEESIVGSPPKDSPASSYRSRSLPRAFAMRDASRRREEEEEEEEKEKKVEEPQAMEPELAAETVPEHIQAEKEENKEKEDKEETPVFNLIPATPAAIPEEVRDAAEAAMKEAQDDKASSEEDKKDHRKTLSDSASIRSRKHSRANSLSDLREEPEESAAPARENSGSLTVDYKGKQPATSDEDDISIVDHPVSASPDQLSINEEVKKTEEPKPAPVVDSPSHGPPPSRGGSIMDDGKSISSQDPKSSPSRKGSFRRMVSGLPTRQPSNDAMKREGSLRRSLTSVIPGGKSKKRDKSAPPSSASSQILKLDTEAPAGSKSTTDVREAIKPPSESINTASPVKPDETPAVSPVAGPSSDDAQSTREEEKPKPASRRSSWWGISKELKRKNSIAKSDKGEEVTTRPATATSTTAPVVAPATPEGKGKEVERPAEVTTEASPAPPPAPAIPTEGVAKSDAPAAIAEPATTETPATETPAAAAASPPKPMLKERVERVKSIKSFWGRKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4U3</accession>
    <name>D5G4U3_TUBMM</name>
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        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="ORF">GSTUM_00000201001</name>
    </gene>
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        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
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        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
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            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429991">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002835333.1"/>
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    <dbReference type="AlphaFoldDB" id="D5G4U3"/>
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    <dbReference type="KEGG" id="tml:GSTUM_00000201001"/>
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    <dbReference type="InParanoid" id="D5G4U3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18186">
        <property type="entry name" value="BTB_POZ_ZBTB_KLHL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47843:SF2">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47843">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="2">
        <location>
            <begin position="114"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ79536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="324" mass="36087" checksum="CBB40C39378BD7A9" modified="2010-06-15" version="1">MAMRKKGRKNASGLYHWSGSARRTGSTLQADTTSALQATSALQAAITPPETPQATSHWQATGAFEASNVPEGSSHPPKIADDTPEQSDATPASQMIPEASKQSPPIPFDKFASSDIFTIRAGTELQAFHVHKDLLAGLSDEMRNHVYNDMREGKANVMDLKHISPETMHRFMEFCYSGDYLFPPGAERKDPLKYKEATEALPILLTHARLYVFAEMFNIVSLKRLSADKIIAITPLFGRLEERAHGLAVISLMEFVLDNIPATTETTDRLVKFLARYSGYFVEQIGGYPEFHALLASSAREDFFRVFCKWVRAGGDREPWTDIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5E9</accession>
    <name>D5G5E9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
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    </gene>
    <organism evidence="1 2">
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        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
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            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
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            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835585.1">
        <property type="nucleotide sequence ID" value="XM_002835539.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5E9"/>
    <dbReference type="GeneID" id="9188161"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004302001"/>
    <dbReference type="HOGENOM" id="CLU_2484954_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5E9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ79742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="87" mass="9727" checksum="E46E8417DD7FC024" modified="2010-06-15" version="1">MCHYRNITFSCGCQTEENTLVCGYVHDDVAQCMADTQADEVSVTRACGRRCCKNAPPRGSNLKPQDNHKTNHCHVEWPKENAATSEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5J5</accession>
    <name>D5G5J5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Rho GDP-dissociation inhibitor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00004368001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Rho GDI family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835631.1">
        <property type="nucleotide sequence ID" value="XM_002835585.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5J5"/>
    <dbReference type="FunCoup" id="D5G5J5">
        <property type="interactions" value="421"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G5J5"/>
    <dbReference type="GeneID" id="9183774"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004368001"/>
    <dbReference type="eggNOG" id="KOG3205">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076228_1_0_1"/>
    <dbReference type="InParanoid" id="D5G5J5"/>
    <dbReference type="OMA" id="YKPTAAK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005934">
        <property type="term" value="C:cellular bud tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005094">
        <property type="term" value="F:Rho GDP-dissociation inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032889">
        <property type="term" value="P:regulation of vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007266">
        <property type="term" value="P:Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.50.30:FF:000001">
        <property type="entry name" value="Rho GDP-dissociation inhibitor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.50.30">
        <property type="entry name" value="Coagulation Factor XIII, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000406">
        <property type="entry name" value="Rho_GDI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024792">
        <property type="entry name" value="RhoGDI_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10980:SF3">
        <property type="entry name" value="LD16419P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10980">
        <property type="entry name" value="RHO GDP-DISSOCIATION INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02115">
        <property type="entry name" value="Rho_GDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00492">
        <property type="entry name" value="RHOGDI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00054143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00071407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ79788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="202" mass="22747" checksum="6B32F60CBA701C99" modified="2010-06-15" version="1">MASHADDELKPSTTEGYKVGEKKSVDEYAKLDAEDESLARWKQSLGIGSGSAGGSLGDPGDKRKVVILQLCLLISGRPDVVISLDTPAALEELGNKPFIIKEGAEYRMKVRFRVQHEVISGLRYLQLVKRKGIKVDKSDEMMGSYGPNTSETPYYEKIFSEEEAPSGMIYRGRYDAVSKFMDDDHNTHLEFKWNFDIKKSWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5U7</accession>
    <name>D5G5U7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00001583001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="2">Binds 1 Fe cation per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the pirin family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430002">
        <property type="protein sequence ID" value="CAZ79890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835733.1">
        <property type="nucleotide sequence ID" value="XM_002835687.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5U7"/>
    <dbReference type="STRING" id="656061.D5G5U7"/>
    <dbReference type="GeneID" id="9187568"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001583001"/>
    <dbReference type="eggNOG" id="ENOG502QQ5A">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045717_0_2_1"/>
    <dbReference type="InParanoid" id="D5G5U7"/>
    <dbReference type="OMA" id="TPWHPHR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02247">
        <property type="entry name" value="cupin_pirin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02909">
        <property type="entry name" value="cupin_pirin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012093">
        <property type="entry name" value="Pirin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008778">
        <property type="entry name" value="Pirin_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003829">
        <property type="entry name" value="Pirin_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13903:SF8">
        <property type="entry name" value="PIRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13903">
        <property type="entry name" value="PIRIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02678">
        <property type="entry name" value="Pirin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05726">
        <property type="entry name" value="Pirin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006232">
        <property type="entry name" value="Pirin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pirin N-terminal" evidence="4">
        <location>
            <begin position="22"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Pirin C-terminal" evidence="5">
        <location>
            <begin position="177"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR006232-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ79890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="302" mass="33426" checksum="04E89A78638ACE19" modified="2010-06-15" version="1">MIVARTIAKAFKAIEKAEGAGAKVRRSVGTSQLKNFSPFLMLDHFSVGSEAGFPDHPHRGQETITYLLHGSIDHEDFAGHKGTIHPGDLQFMTAGRGIMHAEMPGKNGDGSPNVGLQLWVDLPKHLKFCEPRYRDLAAKEIPTVTTDNEKVFIKIISGTSHGVDSVRDLAYTPVWILDVTMKPGARLEQQLPKGWNAFAYTLKGKAAFGANGEKRVGEFYNVVFEQEGDGVFAATDDNAEEDAHFIIIAGQPLDQPVIQYGPFVLNTEEQVYQAMLDYQTHSNGFERAKHWQSEIGKRMTGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5G651</accession>
    <name>D5G651_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00001771001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430007">
        <property type="protein sequence ID" value="CAZ79994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835837.1">
        <property type="nucleotide sequence ID" value="XM_002835791.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G651"/>
    <dbReference type="GeneID" id="9187673"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001771001"/>
    <dbReference type="HOGENOM" id="CLU_2529106_0_0_1"/>
    <dbReference type="InParanoid" id="D5G651"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ79994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="84" mass="9473" checksum="F551E45636B25985" modified="2010-06-15" version="1">MDGVLNAACGIEGNAPFVLSTGLDRFYPYFVNRTHRPYAIPNTTGLHQTYFPTGDPSLERPIHYLLLWDILTGVEVHQRDVITV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6F6</accession>
    <name>D5G6F6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00004462001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430009">
        <property type="protein sequence ID" value="CAZ80099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835942.1">
        <property type="nucleotide sequence ID" value="XM_002835896.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6F6"/>
    <dbReference type="GeneID" id="9181184"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004462001"/>
    <dbReference type="HOGENOM" id="CLU_1403361_0_0_1"/>
    <dbReference type="InParanoid" id="D5G6F6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="194" mass="20181" checksum="B3B15A5674928882" modified="2010-06-15" version="1">MSSFLNDPLKGLREIFFSTLRVVTCGPILIRDLVKAADPVLGPASKGSRALIPLSLTISALCVLAAENKMDELAKQICSVALGSAGAAIGSAIGILGGPVGMVVGAALGGIAGGLLGDFGYAKFKQWLFTEGEDGVRPIDRIADKLRAATDTLRVVAEISSIIMHGQTEVFKALLEKWRRMGGLEGGRGFGGIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6V9</accession>
    <name>D5G6V9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00002302001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430017">
        <property type="protein sequence ID" value="CAZ80252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836095.1">
        <property type="nucleotide sequence ID" value="XM_002836049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6V9"/>
    <dbReference type="STRING" id="656061.D5G6V9"/>
    <dbReference type="GeneID" id="9182476"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002302001"/>
    <dbReference type="eggNOG" id="KOG0733">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_8_3_1"/>
    <dbReference type="InParanoid" id="D5G6V9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990275">
        <property type="term" value="F:preribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000018">
        <property type="entry name" value="Cell division control 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000365">
        <property type="entry name" value="Ribosome biogenesis ATPase RIX7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015415">
        <property type="entry name" value="Spast_Vps4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF171">
        <property type="entry name" value="NUCLEAR VALOSIN-CONTAINING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09336">
        <property type="entry name" value="Vps4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00830">
        <property type="entry name" value="ENDOLAPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="125"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="381"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="13"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="25"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="61"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ80252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="648" mass="70729" checksum="81D1EBE130927455" modified="2010-06-15" version="1">MAIEKVLATIRDEVAGGNSAGSGSDEFEGLDEDSVVEPKGSNTMNKPVAGKIVPALSSPLKRKDLEEVNDGEARKRPKQNNSGVAPKTIYRNDIGGMEDIMDDLLELIVMALVHPEVYQLTGMPPPLGVLFHGPPGCGKTMLANIIAEEVGRPFIAISAPSIVSGMSGESEKRLRELFEEAREKAPCLMFIDEIDAIMLKRDNTERGMDRRLVAQMLTCMDDLTLEKTGGKPVVIIGATNQPDSPDPALRRPGRFNREIHLSIPDEVGREKILRVVCGKLKLPSDFDFKRLAKETPGFVGGDLSALAAGANAVAIRRLYEALRNPIAATDSLEVTRVAGSGLRGSIQQFLKAYPDPLTEKQINSHFPIKNPEIFRSAGISLPAGVLLWGPPGCGKTLLAKAVANESRANFISIQGPELLSKYVGESERAVRQVFSRTRACIPCVIFFDELDGLVPRRNDSHSESTSRVVNTLLTELDGRDNRKGIYVIGATNRPDVIDPAMLRPGRFDKLLFIDLPDKDGRLEILKAVTKMTPLSNDVDLRAIAEDNRCKGLSGADLTALVRQATIESLLRCRFTDVGGVEEGKITSNLQALVTAEDFEKALGRVRRSVSEDDREEYREFAAAFGLQGGRRGSGRCTKYRSFPIPISM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5G736</accession>
    <name>D5G736_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00002324001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430021">
        <property type="protein sequence ID" value="CAZ80329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836138.1">
        <property type="nucleotide sequence ID" value="XM_002836092.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G736"/>
    <dbReference type="STRING" id="656061.D5G736"/>
    <dbReference type="GeneID" id="9182520"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002324001"/>
    <dbReference type="eggNOG" id="KOG0024">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_11_0_1"/>
    <dbReference type="InParanoid" id="D5G736"/>
    <dbReference type="OMA" id="MGLMMLE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08234">
        <property type="entry name" value="threonine_DH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050129">
        <property type="entry name" value="Zn_alcohol_dh"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43401">
        <property type="entry name" value="L-THREONINE 3-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43401:SF2">
        <property type="entry name" value="L-THREONINE 3-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like N-terminal" evidence="6">
        <location>
            <begin position="30"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="5">
        <location>
            <begin position="175"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ80329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="346" mass="37623" checksum="C3F6FED2FE480D84" modified="2010-06-15" version="1">MSLPATMKALRYDKPESYAVVEVPLPTLRDNDVLIKVKACGVCGTDLHIHEGEFIAKFPLIPGHETVGVVAAVGKDVKGFEVGERVCADNSELCNECFYCRRGQLLLCEKFEAHGVTMDGGFAEYCAYPAGKVFKIQNLTDVDATLLEPASCAAHGLEKIAPKIGSSVLMFGAGPTGLCLAQLLRHNGASHVVIAAPEGLKMDLAKKLDCADIYVPLSRSNPQEQFEKIKNDNPYGFDIVVEATGSPKILEDAINYVRRGGKLVVYGVYSDSARVSWPPSKIFGDEITIIGSFSETYMFPATIGYLDTGKVRVEGIVNKTYKLEQWGECLEAMRNKSAIKAAIVFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7K3</accession>
    <name>D5G7K3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00004593001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430031">
        <property type="protein sequence ID" value="CAZ80496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836305.1">
        <property type="nucleotide sequence ID" value="XM_002836259.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7K3"/>
    <dbReference type="FunCoup" id="D5G7K3">
        <property type="interactions" value="253"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G7K3"/>
    <dbReference type="GeneID" id="9181404"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004593001"/>
    <dbReference type="eggNOG" id="KOG0062">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1242">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002848_0_1_1"/>
    <dbReference type="InParanoid" id="D5G7K3"/>
    <dbReference type="OMA" id="EDHRKFG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008079">
        <property type="term" value="F:translation termination factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006449">
        <property type="term" value="P:regulation of translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03221">
        <property type="entry name" value="ABCF_EF-3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000076">
        <property type="entry name" value="Elongation factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000193">
        <property type="entry name" value="Probable Elongation factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050611">
        <property type="entry name" value="ABCF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211:SF14">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY F MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24984">
        <property type="entry name" value="HEAT_EF3_GNC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24987">
        <property type="entry name" value="HEAT_EF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="HEAT" evidence="7">
        <location>
            <begin position="207"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="432"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="676"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="898"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="898"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="928"/>
            <end position="937"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ80496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="972" mass="106595" checksum="D66F19D786556586" modified="2010-06-15" version="1">MPGVLDATSPTSSPRSSTNQPLEMLLTDAAISDYLSAVFSADSSQGSLDAALALCSAILSDPATAHHSFPTLFPPLAKAASDKKSGTRRESAMIVYGALYESFIPKSPITEVLLLKETLPIVLDGLADKGAVVREASQYAIDAMLALLKEEALVVGLVRVLMTYVKGSGSKWQGRVAALQLIGRVAEKAVTRDGFLKEVMGRELETLIPVVEGGMHDLKNEVSRAAAKAMTSLAKLLSNDDVARHIPTLIKTMSDPSKETLQKAIHDLSQTTFIAVVTSPVLSLLTPLLERSLSSPQTSQDVLRQTVVVVENLTKLVHDPVEAREFLPRLQPGVKKIEDTAALPVVRSLAKDANATMVKAMKVGTGTDVPERISVDEFKRISGCITVYLEHFMAQDDAKRVAAEIEQWARDETLNRFGNGIGEVDDEHKHEVEIVNAEFSLAYGGMMLLTHTNLRLFQGHRYGLCGRNGAGKSTLMRSISQGKLEGFPSKDELRTCFVEHKERDERDMSIVGYIRLALEENGDGNISDEEIVETLKSVGFEGERAEMNIGQLSGGWKMKLGLAEAMLKRAQVLLLDEPTNHLDVSNVQWLQDYLKLHTEITSLIVSHDSKFLDEVCTDIIHYENKKLVYYKGNLSEFVRKRPEGKAYYSLDASNVSFKFPSPGLLTGVKSATRSIIKMTNVTYTYPTATRPSLYNVTTSLSLSSRVAIIGGNGAGKSTLVKLLTGEMVPQEGRVEKHPNLRVGYMAQQSLHHLVQDFDDAESAREGLGYRDLDVASIEKHFEDIGLDKEIAMNNEIGGLSGGQKVKVVIAAAMWPKPHLLILDEPTNYLDRESLGALATAIKEFRGGVCLISHDAQFVSQLSNEEWHIEAGRMVRKTSNGSNMNLSLPLDLNSRPGSSAVSLSSSTVSSAANTDAEDNNGEGMEFKSIKRGKKKKYTRAQLKEREVRRRLRYLEWLNSPKGTPKPADTDDES</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7L8</accession>
    <name>D5G7L8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00004614001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430031">
        <property type="protein sequence ID" value="CAZ80511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836320.1">
        <property type="nucleotide sequence ID" value="XM_002836274.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7L8"/>
    <dbReference type="STRING" id="656061.D5G7L8"/>
    <dbReference type="GeneID" id="9186990"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004614001"/>
    <dbReference type="HOGENOM" id="CLU_2428660_0_0_1"/>
    <dbReference type="InParanoid" id="D5G7L8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="3">
        <location>
            <begin position="18"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="63"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ80511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="91" mass="10257" checksum="2A49A6B3EB1B0456" modified="2010-06-15" version="1">MADDTKQNRIKRLKTARACDQCRRGRSRCDIAALPASSPENSNCQRCMEHGFECTSVLSIKETRGKRTTAQKQDLNSPTRESPTTEARQVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7P8</accession>
    <name>D5G7P8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00004672001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Putative mitochondrial redox protein which could be involved in the reduction of small toxic molecules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FMP46 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430031">
        <property type="protein sequence ID" value="CAZ80541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836350.1">
        <property type="nucleotide sequence ID" value="XM_002836304.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7P8"/>
    <dbReference type="GeneID" id="9187020"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004672001"/>
    <dbReference type="eggNOG" id="ENOG502S4MH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126094_0_0_1"/>
    <dbReference type="InParanoid" id="D5G7P8"/>
    <dbReference type="OMA" id="IVDWNNG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012882">
        <property type="entry name" value="Fmp46"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28071">
        <property type="entry name" value="REDOX PROTEIN FMP46, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28071:SF1">
        <property type="entry name" value="REDOX PROTEIN FMP46, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07955">
        <property type="entry name" value="DUF1687"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ80541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="128" mass="13767" checksum="66B856C5A1B955BE" modified="2010-06-15" version="1">MFRFRKTLDVLTLFHAPASAASKRILETLRSSPAAHKKSFELDVVEAPTVPTPTQLASILEFTGKSRVGEVVPGARSEDDAARMLKVEEEGGAGRMVRPLLVDWNNGRAVVGGDEGAVLRLLETLPGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7R6</accession>
    <name>D5G7R6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00004693001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430031">
        <property type="protein sequence ID" value="CAZ80559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836368.1">
        <property type="nucleotide sequence ID" value="XM_002836322.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7R6"/>
    <dbReference type="STRING" id="656061.D5G7R6"/>
    <dbReference type="GeneID" id="9181378"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004693001"/>
    <dbReference type="eggNOG" id="ENOG502S404">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_698660_0_0_1"/>
    <dbReference type="InParanoid" id="D5G7R6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47783:SF1">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47783">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="3">
        <location>
            <begin position="44"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="82"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="96"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ80559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="395" mass="43057" checksum="B0918050495B37A9" modified="2010-06-15" version="1">MPQSVKRSLSTPNVREDASIQAAGGSYSTDKRRNKLGYHRISVACGHCRRRKIRCIAAKDDPSGRCSNCIRLKKDCNFYPVENTDRRHKSPPKLDTPNNNDGGSAGSSPSPVFGEGQIHQNPNEYPESVPVTPTSDQPMSNFGDNYQPNRDTGLPPRIPISQSASVSRRPSMAQLNSGTEEIFVGVPQWVESFQPDMLGCVDQHSYEDLSGIYYRLNSPPICGAHYPQHPLHNMSSMASLTTPESLDSYDGRIYSQGPSRMGSIDQGMGSVFSYPTTHSPEAAEFGVVLDSRSASAPTPPLTASISEASQFGAPGESGYGQRYNMAQWVDQTSNGIHFDTESVAKEEDGVATDVPYLFPVEDGYSMNGDFHQLAHPGQHAGPIVKPDQELGLDHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7W6</accession>
    <name>D5G7W6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00002682001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430032">
        <property type="protein sequence ID" value="CAZ80609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836418.1">
        <property type="nucleotide sequence ID" value="XM_002836372.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7W6"/>
    <dbReference type="GeneID" id="9181428"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002682001"/>
    <dbReference type="HOGENOM" id="CLU_1628254_0_0_1"/>
    <dbReference type="InParanoid" id="D5G7W6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ80609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="163" mass="17038" checksum="A22E31E659EE1C01" modified="2010-06-15" version="1">MSSNPAAGSSTMAYYSGHPLTDEEIANTPAIYTPVIGPTHISMSYADSTCSSNSDVSPFFYEGIWLEGPDAPQGVLTEQTPLLSAPVPFDPELDGFQLDIAGAVPLKPGDLESGSLAQVENVVGLGTRLSSVIGRLWGWILGIFQSCGLVGDTGDENGEGEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5G837</accession>
    <name>D5G837_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00002794001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430039">
        <property type="protein sequence ID" value="CAZ80680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836489.1">
        <property type="nucleotide sequence ID" value="XM_002836443.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G837"/>
    <dbReference type="GeneID" id="9184153"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002794001"/>
    <dbReference type="HOGENOM" id="CLU_3144028_0_0_1"/>
    <dbReference type="InParanoid" id="D5G837"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ80680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="49" mass="5410" checksum="C8EE581F96957A72" modified="2010-06-15" version="1">MNEAWRLVELCLQRVGEGTNQVASNLKPVPVRGKLTTSKEVSPGVVVYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5G853</accession>
    <name>D5G853_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00002834001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TfdA dioxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430040">
        <property type="protein sequence ID" value="CAZ80696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836505.1">
        <property type="nucleotide sequence ID" value="XM_002836459.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G853"/>
    <dbReference type="FunCoup" id="D5G853">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="GeneID" id="9184259"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002834001"/>
    <dbReference type="eggNOG" id="ENOG502QT05">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036005_0_0_1"/>
    <dbReference type="InParanoid" id="D5G853"/>
    <dbReference type="OMA" id="RHFGKPN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016706">
        <property type="term" value="F:2-oxoglutarate-dependent dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.130.10:FF:000003">
        <property type="entry name" value="Alpha-ketoglutarate-dependent taurine dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.10">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051323">
        <property type="entry name" value="AtsK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042098">
        <property type="entry name" value="TauD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003819">
        <property type="entry name" value="TauD/TfdA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30468">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT SULFONATE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30468:SF30">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT TAURINE DIOXYGENASE (AFU_ORTHOLOGUE AFUA_7G06030)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02668">
        <property type="entry name" value="TauD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TauD/TfdA-like" evidence="8">
        <location>
            <begin position="78"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="27"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ80696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="361" mass="40833" checksum="0F86274530EAB57B" modified="2010-06-15" version="1">MAPHSLEPTEEAASYDINLPYKPASNNDEHKQEEVSKYPQYLPQWEPVFFPQCPEFDFLDPALRADKEKKILLKDAQVKHISPKMGTELSGVQLSSMSDAAKDELALLVSERKCVVLRDQDFADWGPSNQQDFASYFGKPNYQPVTGSIPGFPGFHIIYRDGNQAEIDKFFEHKLTSTLWHQDVSYERQPPGYVILCILQAPEVGGDTVFASTAEAYNRLSPGFQSMLEDLRATHSSEKMCNFAKANGGLCRKDPIRTSHPIVRVHPVTGEKSLFVNGEFIRGIDGWKDPESDILLNFLIDHITKGHDFQVRLSWKPKSVVIFDNRSTCHTATVDYDARSDKDRHMFRLASMAEVPIPAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5G883</accession>
    <name>D5G883_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00002926001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430041">
        <property type="protein sequence ID" value="CAZ80726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836535.1">
        <property type="nucleotide sequence ID" value="XM_002836489.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G883"/>
    <dbReference type="FunCoup" id="D5G883">
        <property type="interactions" value="1012"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G883"/>
    <dbReference type="GeneID" id="9188328"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002926001"/>
    <dbReference type="eggNOG" id="KOG0179">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035750_1_0_1"/>
    <dbReference type="InParanoid" id="D5G883"/>
    <dbReference type="OMA" id="CSGCWCD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000027">
        <property type="entry name" value="Proteasome subunit beta type-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF2">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA TYPE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0647">Proteasome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ80726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="257" mass="28062" checksum="285B6EB27E75652D" modified="2010-06-15" version="1">MSLPFMQPLGYDQSYSFHTPTSKASGGYTQHSFNPYTDNGGTIIGITGEDFVVLAGDTRHTSGYNINSRTEKKVYRLGEDNSLVLATVGFGADSKDVAETMMRAVNDFKFNHNLKNMSVSAAAQYLSTFLYGNRFFPKYANCVLAGLDKDGRARLYSYDSVGSYSKQGESRASAGSASSLIVPFLDNQVEFKNQFVPSSKGQEERPVVPLDPVTVAKLVKDAFTSATERHIEVGDGLQIITVTKDGVSEEVVDLKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8E4</accession>
    <name>D5G8E4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Non-homologous end-joining factor 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00004779001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the XRCC4-XLF family. XLF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430042">
        <property type="protein sequence ID" value="CAZ80787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836596.1">
        <property type="nucleotide sequence ID" value="XM_002836550.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8E4"/>
    <dbReference type="STRING" id="656061.D5G8E4"/>
    <dbReference type="GeneID" id="9186007"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004779001"/>
    <dbReference type="eggNOG" id="ENOG502SCQK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_792715_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8E4"/>
    <dbReference type="OMA" id="NAREEWW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032807">
        <property type="term" value="C:DNA ligase IV complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045027">
        <property type="term" value="F:DNA end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22285">
        <property type="entry name" value="HD_XLF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.210.10">
        <property type="entry name" value="DNA double-strand break repair and VJ recombination XRCC4, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052287">
        <property type="entry name" value="NHEJ_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053829">
        <property type="entry name" value="XLF-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015381">
        <property type="entry name" value="XLF-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038051">
        <property type="entry name" value="XRCC4-like_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32235">
        <property type="entry name" value="NON-HOMOLOGOUS END-JOINING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32235:SF1">
        <property type="entry name" value="NON-HOMOLOGOUS END-JOINING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09302">
        <property type="entry name" value="XLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21928">
        <property type="entry name" value="XLF_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XLF-like N-terminal" evidence="9">
        <location>
            <begin position="7"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="XLF-like coiled-coil region" evidence="10">
        <location>
            <begin position="133"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="222"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="331"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="244"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="305"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF09302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF21928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ80787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="350" mass="39047" checksum="00D39E91A173806D" modified="2010-06-15" version="1">MPLTKTPWHPLPLTISNRSAPPLLLQSTFTSTTYTIHLTDLVNIWSESLSKREILARAERNSTAIDPSEDVSQLPILLEKLEYALGRKRDDDAVDVEIFLGKSGLRLKATVELPKPLGDLKWVFELQPVGGVELVNLLVLPYLSEVATLRDMVESLMTVVKEKDTVLERLVEGMGEAGMDVGAMVGGGRRRRGLDKFDVGRWRGEFLGGDRRAGEVVSEVFEQNSEPEIPGVKGLGGEAGEWWRGLDEDGGGEEKEKEPRKKFEPGGGAQERWLQNPIPGKPVSDTDDDEFEVRRTPPRASERTPQSIKKPQSISPPSFRRKLSPCFKIRLAIQNSTPTHQASHREDRWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8L3</accession>
    <name>D5G8L3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00002998001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430047">
        <property type="protein sequence ID" value="CAZ80856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836665.1">
        <property type="nucleotide sequence ID" value="XM_002836619.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8L3"/>
    <dbReference type="FunCoup" id="D5G8L3">
        <property type="interactions" value="185"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G8L3"/>
    <dbReference type="GeneID" id="9186224"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002998001"/>
    <dbReference type="eggNOG" id="KOG0104">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005965_5_0_1"/>
    <dbReference type="InParanoid" id="D5G8L3"/>
    <dbReference type="OMA" id="SRTPMIQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034663">
        <property type="term" value="C:endoplasmic reticulum chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10230">
        <property type="entry name" value="ASKHA_NBD_HSP70_HYOU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639">
        <property type="entry name" value="HSC70CB, ISOFORM G-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639:SF3">
        <property type="entry name" value="HYPOXIA UP-REGULATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072011" evidence="6">
        <location>
            <begin position="28"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="810"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="810"/>
            <end position="820"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ80856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="831" mass="91404" checksum="3A04625981CE2457" modified="2010-06-15" version="1">MRPISASPAALAGLLVLSVSSLASASAAVLGIDFGQEFIKAALVKPGIPLEIVLTKDTKRKEAAAVGFKPASNGKEGEFAYPERLYGADAVNLAARFPHDVYPGLKKVMGQHITDDSVMAYWARNPALSVVPNLFRSTVAFQSSSAPPVDSGEGEFTVEELLAMQFKSIVYNAKALAGANTRIRDVVFTVPAFLKAEERSSLKLAAELAGLKVMSFVSDGLAVGINYATTRTFNESSPEYHIVYDMGAGSTTATLLRFQGRSVKDVGRFNKTVQEVTVLGIGYDTELGGDIFNQEIFEILLDDFVNNPKAKGLLEEDPDLKNTVRTNGRAASKLWKEATRVRQILSANTDTVASIESLYKDIDFRSSKITRATFEKLLAQYEARITKPITDAISNANVALSEVNTLIVHGGAVRTPFVGKKIEDIIGGAEKISKQVNPDESAVLGATFKGAAESGAFKVKEIRTQDIGAFGVSIKYKKDAEAKKETVQQIFPFYSKIGSEKVIPFSRNSELTFNTVHNLPPRINDPNQKPRQELLETIETSNLTSSVKKLQDDFGCAENDIVTKFGIVLNPKDSLPKVIRFWVECGVEVAPEAPKRDAGGAFEDVKGFFGYGKKDASKSTKSADSQPRAIRRIEKIPVKYTITKAGFPNIPEKDMTSLILKIRGFDKHDKERKDLEEARNNLEAYTYRARGYLSDDAFIAVSTEEQRERLDAMISEASDWLYGDGYTAGLKEVHQKIKQLEALEAPISKRRTEKDSLPEALKGLNNILISMKGFIEREESLAEAQSDDSYSFDLKPLKKAYEKERAWVEEKSAAQEKLQPHEGPILTSEEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8V7</accession>
    <name>D5G8V7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00004858001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430053">
        <property type="protein sequence ID" value="CAZ80950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836759.1">
        <property type="nucleotide sequence ID" value="XM_002836713.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8V7"/>
    <dbReference type="GeneID" id="9181233"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004858001"/>
    <dbReference type="eggNOG" id="ENOG502SAHG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_903703_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8V7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40623:SF1">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40623">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="13"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="211"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="257"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="268"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="298"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ80950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="308" mass="32147" checksum="C4AE204AF49DA725" modified="2010-06-15" version="1">MGSFGDLELGAKYGIVLGSLVVLVFTAGFLKLLYIRHQAKKFNEEAKAKAANEEGKTEQIALNQREEDEGDLFGIRALERGFTGGVAQSRPTTPNTATNRSTSTLVPSAVNIPFGQKSAVSFAGSSRSETDSTLTIEVGNPKGITISNGRTSPRAGDVAVDMNLNVPESPRMPRSPDSSKAPSIYSTRSSPTLKPISGVAIPSSGLGMQFGQDGTVSPRMQAKAPTRVVTGSSRGMTVPSDSASLVDGSDGTPPLSPSSSSYSVTPSEGKSTNYGDRSGSPSASQRPYQGHNPSIRVDGPDHPCETLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>D5G925</accession>
    <name>D5G925_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00004955001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430053">
        <property type="protein sequence ID" value="CAZ81018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836827.1">
        <property type="nucleotide sequence ID" value="XM_002836781.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G925"/>
    <dbReference type="GeneID" id="9181301"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004955001"/>
    <dbReference type="HOGENOM" id="CLU_3242466_0_0_1"/>
    <dbReference type="InParanoid" id="D5G925"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ81018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="43" mass="4803" checksum="21A2AE249F49812F" modified="2010-06-15" version="1">MRDCSGRLVGVGGVPRMVVRGRRMKERWRWGGREEGVSGGELS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9E1</accession>
    <name>D5G9E1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="15">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">GSTUM_00003266001</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Mel28</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Component of the NuA4 histone acetyltransferase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430059">
        <property type="protein sequence ID" value="CAZ81134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836943.1">
        <property type="nucleotide sequence ID" value="XM_002836897.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5G9E1">
        <property type="interactions" value="816"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G9E1"/>
    <dbReference type="GeneID" id="9182885"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003266001"/>
    <dbReference type="eggNOG" id="KOG0384">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000315_29_0_1"/>
    <dbReference type="InParanoid" id="D5G9E1"/>
    <dbReference type="OMA" id="WVQIRDD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140658">
        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034728">
        <property type="term" value="P:nucleosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18659">
        <property type="entry name" value="CD2_tandem"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17993">
        <property type="entry name" value="DEXHc_CHD1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000130">
        <property type="entry name" value="Chromodomain-helicase-DNA-binding protein 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1440">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056302">
        <property type="entry name" value="CHD1-2/Hrp3_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023779">
        <property type="entry name" value="Chromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623:SF14">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23588">
        <property type="entry name" value="HTH_CHD1_Hrp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00598">
        <property type="entry name" value="CHROMO_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Chromo" evidence="12">
        <location>
            <begin position="241"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="Chromo" evidence="12">
        <location>
            <begin position="341"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="440"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="742"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="990"/>
            <end position="1058"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1259"/>
            <end position="1444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="23"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="35"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="95"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="178"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="990"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1271"/>
            <end position="1281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="1320"/>
            <end position="1332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1333"/>
            <end position="1382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAZ81134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1444" mass="163113" checksum="B3235CB7BB70F923" modified="2010-06-15" version="1">MSPASSSPSEGPLSDAEEDAGAQQESISTPESGNQDEVVSSASSDEGGDGGQSEDGDYEIETPAEEPADDHNGDSDDSDIPVKSAGSLRRTAKSKPADDFHENPDLYGLRRSERARPAARPVVDTTSDSDDSEPKSNRKRRRTVTAPRSSKSSKRNTPALGCGSGDSDDDEDDYGVRKASKSRGKRRRVVVSDAPSADGLRFSTRKAAKITSYNEDGDDDFEEEEDGAIAYECADPGADVGGIDSILDHRPREGIDSTDPIMERGELQFYIKWQGLSHYHSTWETAEALAGCKGIRKLDNYMKKVVLEDYYARTNPFITREEVENMTLERERLRESLAEFVHVERVIGDQVNEEGNREYMIKWKRLAYENCTWESAELITEIAPDEIDKYRVREKTLPYSKKSESDVRTRRPYKKLEGQPSYIKGGELRDFQMKGLNWLAYNWTNGNNGILADEMGLGKTVQTVAFMSWLRHDRHQHGPFLVVVPLSTVPSWAETLENWAPDMNFIVYTGTGKAREVIRKYEMFADPGMTKVKFNCMVTTYEYILNDFATLGNIKWQFLAVDEAHRLKNKESALYDKLNVFKAPCRLLITGTPLQNNLKELGALVDFLMPGKISIDNDVDLQSKDAGRQIEQLQQALKPYMLRRVKKSVEKSLPGKTEKIIRVELSDVQTEYYKAIITRNYAALNAGATGPKQSLLNIVMELKKISNHPFMFPPAEQRILGGSNRREDVLKALIMSSGKMVLLDQLLTKLKADNHRVLVFSQMVHMLDILADYLNLKGFSFQRLDGTIAAGPRRIAIDHFNAPESPDFCFLLSTRAGGLGINLMTADTVILFDSDWNPQADLQAMARAHRIGQKAHVMVYRLVSKDTIEEEVLERARNKMILEHLVISLGVTDKGITDKVKKKTDRLESAELSAILKARASKMFEATDNQKKLEELKIDDILLNAEDHITQIEPGLGGEGGDEFLKQFEVTDFKAEVSWDDIIPKEELDAIKSEEQKEEEQAFLKEQIDMNARRKRKSATNGELEGRSAKRRAKELSAAHIDSEDDEPEKDPRRPLTGPEIRNLYRAYARYGCLEQCWEQIVKACGLEDRDPDLVKTTIADMTQIGEEAIARQRAIDDGTGKKSKQAILFTYKGCPNINAQTVVERPRELKELRRAVASFSEPLKFRIEDVKPVHNWACEWGTREDAMLCVGIARHGYGSWNLIRDDPELGMGDRFFLEEHRVGKKDEAAKSPGAVHLVRRADYLLGVMRARFEAASIAAPSTARHRRSPDANSRQPQKRNGASAVDRVTKKDRVSLSASPAPSKKYRYQTADPSEKHRPEKHRPEKHRPEKHRPENHRSETHRPEKRRHEEKGRYQEKVRYEEKERYQDRARYQEKGRIQDKTSVSSKKHKSDGHEVDSANRKKAKSSPPPKANAKGNGHVTEVNRPLTAEESVAEVLPSFPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9I9</accession>
    <name>D5G9I9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00003345001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430062">
        <property type="protein sequence ID" value="CAZ81182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836991.1">
        <property type="nucleotide sequence ID" value="XM_002836945.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9I9"/>
    <dbReference type="STRING" id="656061.D5G9I9"/>
    <dbReference type="GeneID" id="9184255"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003345001"/>
    <dbReference type="eggNOG" id="KOG1515">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012494_3_2_1"/>
    <dbReference type="InParanoid" id="D5G9I9"/>
    <dbReference type="OMA" id="CEWDMLM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050300">
        <property type="entry name" value="GDXG_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN C4A8.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081:SF8">
        <property type="entry name" value="ALPHA_BETA HYDROLASE FOLD-3 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="2">
        <location>
            <begin position="76"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ81182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="326" mass="36803" checksum="6367605024287C92" modified="2010-06-15" version="1">MPRPRWFLQAEANFFRTLMNTGMCLHRLSPPIPPQHNFTRNIPMTVSPTKGEISLYFYTPPSYPPRPDSSRKYPVVVNFHGGGFTLGHATDDVRWAASVVSHADAIVVSVDYRLAPEHPFPTAIEDGVDALLYLSAHAEELRIDPLRVGISGFSAGGNMAFTVPLRLHEEYRRQPALPQLVIVAIATWYPSTDFTATRTNRRSTNLRPDKELPKLFTDMFDASYLYPPKSVPLDSPYLSPGVASEELLRELPPVIYMYTCEWDELLQEAEGFRVRLESEGVGKKVFYRRVDGAAHAWDKTVGPGDTRREALYKEVCEGLHSVFYKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9P9</accession>
    <name>D5G9P9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00005014001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430064">
        <property type="protein sequence ID" value="CAZ81242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002837005.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9P9"/>
    <dbReference type="GeneID" id="9185379"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005014001"/>
    <dbReference type="HOGENOM" id="CLU_2414899_0_0_1"/>
    <dbReference type="InParanoid" id="D5G9P9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072655" evidence="1">
        <location>
            <begin position="24"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="92" mass="10230" checksum="E9ACC713331F94C1" modified="2010-06-15" version="1">MIRGSMFGQEAVFLAMLVIGLHSSPCGLPEPQAEAVLWGSWQQHSFMNPNFRTVIVCAGALRVSGIVNFGWLICREKCEYGWRFHSSRLGGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9Q6</accession>
    <name>D5G9Q6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00005028001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430064">
        <property type="protein sequence ID" value="CAZ81249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837058.1">
        <property type="nucleotide sequence ID" value="XM_002837012.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9Q6"/>
    <dbReference type="GeneID" id="9185386"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005028001"/>
    <dbReference type="eggNOG" id="ENOG502S6IQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_566217_0_0_1"/>
    <dbReference type="InParanoid" id="D5G9Q6"/>
    <dbReference type="OMA" id="FATYEEP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="323"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="426"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="20"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="193"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="358"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="494" mass="53753" checksum="795893E8A2A841AA" modified="2010-06-15" version="1">MASVVPPYHYHPPRHPGSFVPTTPRSSTSYYPSSTSTVPTSYSVTGPPSSISTSSSPRLNPGLAAARHVTKTPGKLHKRGRSFLKNKEETVDDVDEGFFSGPGSPPPSFDHFEQPSLSHSHPASVSVAAHKARGKIKPLLKKVSGSRSNSLDLSRSDGGLPNGVGLGIYDGTGYDSSGIEDDSPFAYRHRRNFSQTSGSSPLLVGANQTFSHPKRQLPRRGGYTPDVTQYNTSNESSDESDGEHTLDRTRRNALGGSKLPVPGGLHLNTGRSTPMLPTLSMTDLHQRARTPSIQMTTPISPVSPMDLPSTVFPSNVSQHGIKASFKRSHNRARSSLDKSVPETTPSFEASVTAARLAWEAKEEKKEEKRERKRRRSEAKEEERSRATSRCSGRGRGNSGSSNQQHWSHESDFGDNIAIGGVIKEKVGGFDGSRASSKSRNDSRGRHGTGKKWGLSGKSEAPNPKGKRPGFKKRWLGFVVWVRIGVVRMQRRLGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9U7</accession>
    <name>D5G9U7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00005079001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430064">
        <property type="protein sequence ID" value="CAZ81290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837099.1">
        <property type="nucleotide sequence ID" value="XM_002837053.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9U7"/>
    <dbReference type="STRING" id="656061.D5G9U7"/>
    <dbReference type="GeneID" id="9188533"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005079001"/>
    <dbReference type="eggNOG" id="KOG2734">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017098_0_0_1"/>
    <dbReference type="InParanoid" id="D5G9U7"/>
    <dbReference type="OMA" id="TDWREQE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010467">
        <property type="term" value="P:gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:001136">
        <property type="entry name" value="Beta-catenin-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039678">
        <property type="entry name" value="CTNNBL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013180">
        <property type="entry name" value="CTNNBL1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14978:SF0">
        <property type="entry name" value="BETA-CATENIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14978">
        <property type="entry name" value="BETA-CATENIN-LIKE PROTEIN 1 NUCLEAR ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08216">
        <property type="entry name" value="CTNNBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01156">
        <property type="entry name" value="DUF1716"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Beta-catenin-like protein 1 N-terminal" evidence="7">
        <location>
            <begin position="98"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ81290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="597" mass="66171" checksum="A2CA1BDECA74FB0E" modified="2010-06-15" version="1">MASLDNLFKKPALPSSSSNSSKKRKLESSVDPVAAYKSAKLEAPSNDKGKSRAATVEEGDIDDTAGPSALPVNFFDNAGEEPEDEEGGRFFGGGVNKKTAEILDFMDERDRDDTAAASEKIDVAWLRRTALGFEKKINKNAEMRGKYEDDPHKFMASEADLDAGIKAISVLSEHPELFEEFRKLGCLASLVGLLAHENTDIAIDVVEVISELTDDEVEAEPEQWNAIVDGMIEAQLLEMLTQNLSRLNEGNESDRNGVYHTLSVFENLASQQSLAEQMVRETNITPYLLQRIQARESPISQNKQYAAELLAILLQSSPANRKKLSELSGTDVLLQLLSPYRKRDPVKGGDEEEFVENVFDCLTCVVDELEGKEAFVDLEGVELVLIMLREGKMSKPRALRLLDHAVGGQSGANVCQKLVDAAGLKTVFGMFMKKQDNQTTEHLLGIFAALLTHLPADSASRIRTLAKFVEKDYEKIAKLTKLRTDYALRVAKVDAEIAVQREDLGSVVEEEEMAEEWFSKRLDAGLFCLQMTDRILAWLCAEDDGAKKRIGGLLGRAGGSLRDVRRTLKEQIDGMTEASDEWQQVIKDMLSTLIEFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9U8</accession>
    <name>D5G9U8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00005080001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430064">
        <property type="protein sequence ID" value="CAZ81291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002837054.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9U8"/>
    <dbReference type="STRING" id="656061.D5G9U8"/>
    <dbReference type="GeneID" id="9188534"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005080001"/>
    <dbReference type="eggNOG" id="ENOG502QT8Q">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010065_0_0_1"/>
    <dbReference type="InParanoid" id="D5G9U8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070822">
        <property type="term" value="C:Sin3-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038919">
        <property type="entry name" value="STB2/STB2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059025">
        <property type="entry name" value="STB6_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31011">
        <property type="entry name" value="PROTEIN STB2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31011:SF2">
        <property type="entry name" value="PROTEIN STB2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25995">
        <property type="entry name" value="STB6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="STB6-like N-terminal" evidence="2">
        <location>
            <begin position="70"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="433"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="496"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="572"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="514"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="587"/>
            <end position="600"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ81291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="886" mass="96792" checksum="EFFAA1B3D3C2059E" modified="2010-06-15" version="1">MPTVPVPTNSTATSPLPYNVQSQTPPRKTSGAFTPLGGSFGPGGGGGSGVEVVEGIGGGYVDTLPADRQLFVLCDPVAFRYLEEETCTTVVHRHRTLPGHEVYFVEQWACSRIHPTFVISTYTGDPNHVITIGVLNIPRDEAQWSPRLRVYFRAVTKFHARPKETPYGKLMVTNLSTFPSALTVIPVPDGDARLHREDFIVNEDLKRMGCSGRSALSLSMPADASQTKFHQLYRTSDKIPLYTSVMELVRLCQIALTLFEKLKPEYADGLLCDITERGIYDWWTEFGTEFYNIEPSDGTLGPTTVAALLGMVLGARNRLSSFGAPVPKDAFDLPQLKKAIYHFQRQQRIPRTRRLDRETMDKLQKAAMKNGSGASSGDIFAVPRAIKSTVVDLGGRAVGSSSAKVEGTSVETVDIEKFASHLSGERCKYLWRGKPRKSQSATGGGVGGSAGTQTPPAAVSMSRRNSVGGGSISENEGMIRHSGEFTATGYHVVSNPTNSSLGSSGGGSVTSPGLKEKESEGRDVSDLRKTVFKGMSGRMKGMKDVASAGADYVRGRGHHGRSYAKDFGVYEDSTEHTGTTTPLSGLKESERNEREHKGPSEHPVTSFLKLPFHTDSPAGSTGNINFLSVVSGDGGVATGNHSAAASVYREVMDAEGDDDGEEFVDANLPGRQWIDYDEGRILECGRKRNKSFSEYLKKCHEVRHEEYWPRRLSFSVAEEAVLDIGLLPSLDDDVQDESDLTLDTLSRHALNLQTLLADDRISEWTDRRLTTLENIFAYLDSGAGRLEGDLRRGSEVVNSMGEATRDMIDRQTMDLKAAVREVEALGARLQYELGSVRGQMADVEDAVDGFGRNVEEVEERALGLGEAIERGWGEWLLAVVFGIRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>D5GA72</accession>
    <name>D5GA72_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00005166001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430075">
        <property type="protein sequence ID" value="CAZ81426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837235.1">
        <property type="nucleotide sequence ID" value="XM_002837189.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GA72"/>
    <dbReference type="STRING" id="656061.D5GA72"/>
    <dbReference type="GeneID" id="9185708"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005166001"/>
    <dbReference type="HOGENOM" id="CLU_2514266_0_0_1"/>
    <dbReference type="InParanoid" id="D5GA72"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ81426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="85" mass="9715" checksum="6FBAB9B42254DF3D" modified="2010-06-15" version="1">MPFGKIYSYPKNPRTTALLAVAKENGLEIEIVHEEPDKGVSAEFPLSRAMMDSFCLSALLLPFTLPPRTRRPLCWERLSRTMCQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAJ8</accession>
    <name>D5GAJ8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00003617001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ornithine cyclodeaminase/mu-crystallin family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430078">
        <property type="protein sequence ID" value="CAZ81541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837350.1">
        <property type="nucleotide sequence ID" value="XM_002837304.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAJ8"/>
    <dbReference type="FunCoup" id="D5GAJ8">
        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GAJ8"/>
    <dbReference type="GeneID" id="9187481"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003617001"/>
    <dbReference type="eggNOG" id="KOG3007">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042088_0_1_1"/>
    <dbReference type="InParanoid" id="D5GAJ8"/>
    <dbReference type="OMA" id="WHIRIAL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1780.10">
        <property type="entry name" value="ornithine cyclodeaminase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003462">
        <property type="entry name" value="ODC_Mu_crystall"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023401">
        <property type="entry name" value="ODC_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13812">
        <property type="entry name" value="KETIMINE REDUCTASE MU-CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13812:SF19">
        <property type="entry name" value="KETIMINE REDUCTASE MU-CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02423">
        <property type="entry name" value="OCD_Mu_crystall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="351" mass="37687" checksum="680F5FB20D552613" modified="2010-06-15" version="1">MSTPTPLILTHTTITSLLTSLPAPAIRSIIASLTASLQTLTQPTPIITQPPRQTLQDNIANYTTLIMPCSTPNLTSIKSVTLPTPPAPPSPPNGVITLQDAAGRLLAVLNAEEITAFRTACVSIALLLGRIRYGLAVKKAVVFSTGKQAEWISRLLLKLCPTLGRLSIIGREGNSPEKMEAFVGKFRGGVESGTRVGWKTYGGGIDQDVQDELHEADAIFCCTPSRVELFPVEFLTDEKERYISLIGSYKPHMKELSPGYLRRESVTVVVDSVEACLVEAGEVIQAGLKAEDLMDIGSVVSDLENGLGEKYLGDVVFKCVGLAIMDLIAAEEIVKIAKEQKVGIEIPGFSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAP8</accession>
    <name>D5GAP8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00003723001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430084">
        <property type="protein sequence ID" value="CAZ81591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837400.1">
        <property type="nucleotide sequence ID" value="XM_002837354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAP8"/>
    <dbReference type="STRING" id="656061.D5GAP8"/>
    <dbReference type="GeneID" id="9187762"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003723001"/>
    <dbReference type="eggNOG" id="ENOG502S0FZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065859_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAP8"/>
    <dbReference type="OMA" id="QMGHNDA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037459">
        <property type="entry name" value="RhgT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43695">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_2G17250)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43695:SF1">
        <property type="entry name" value="RHAMNOGALACTURONAN ACETYLESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072671" evidence="3">
        <location>
            <begin position="24"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ81591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="276" mass="29925" checksum="BBDDFE5C45F479B4" modified="2010-06-15" version="1">MKSSGLFAALIALSAIGISSVSAAKILMAGDSTMAHWKSSIQGWGTPFSQYIDLPIINLARGGRSARSYYREGLQAELISQIEAGDYVILEFGHNDGGSPATSDRAPTWGDGAQTTVVKLANGTVETVQTFVTYEKWFIRDVIGKGGFPVVSSTTPNGDAWNPAHTEMLAPNRFVPYAKMAAGNFTEATYIDHYEFVTIAFETLGYTNVYPKLFPNDHTHTSPEGADIVAQAFVNGLKCTNHPLAGYLTYAGNALEKKCRSVALFFFSFSAHERNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAS0</accession>
    <name>D5GAS0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00003758001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430085">
        <property type="protein sequence ID" value="CAZ81613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837422.1">
        <property type="nucleotide sequence ID" value="XM_002837376.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAS0"/>
    <dbReference type="GeneID" id="9187779"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003758001"/>
    <dbReference type="HOGENOM" id="CLU_1797851_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAS0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="144" mass="15946" checksum="1F4500853E172692" modified="2010-06-15" version="1">MGSAVPRRFVVGGSDEVRRESVGHLWLLGRRRVRPGFPNCSAGLRVCYRSAGLQLTASLAVLRSSTGQVRVAQHRRLINNAPSTTFTQACKQSGLSLILLSFSSTYHTVFIILLVSFHPQRNFPPSLPRTRNKFYSPNAFLSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5GB17</accession>
    <name>D5GB17_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Serine/threonine-protein kinase SSN3</fullName>
            <ecNumber evidence="6">2.7.11.22</ecNumber>
            <ecNumber evidence="5">2.7.11.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Cyclin-dependent kinase 8</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Serine/threonine-protein kinase ssn3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="ORF">GSTUM_00005403001</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27 28">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>[DNA-directed RNA polymerase] + ATP = phospho-[DNA-directed RNA polymerase] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10216"/>
            <dbReference type="Rhea" id="RHEA-COMP:11321"/>
            <dbReference type="Rhea" id="RHEA-COMP:11322"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43176"/>
            <dbReference type="ChEBI" id="CHEBI:68546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the SRB8-11 complex, a regulatory module of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="6"/>
    <dbReference type="EC" id="2.7.11.23" evidence="5"/>
    <dbReference type="EMBL" id="FN430086">
        <property type="protein sequence ID" value="CAZ81710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837519.1">
        <property type="nucleotide sequence ID" value="XM_002837473.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GB17"/>
    <dbReference type="FunCoup" id="D5GB17">
        <property type="interactions" value="1007"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GB17"/>
    <dbReference type="GeneID" id="9187951"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005403001"/>
    <dbReference type="eggNOG" id="KOG0666">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_181_6_1"/>
    <dbReference type="InParanoid" id="D5GB17"/>
    <dbReference type="OMA" id="YFKNGGP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990508">
        <property type="term" value="C:CKM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008353">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060258">
        <property type="term" value="P:negative regulation of filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070481">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, non-stop decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031648">
        <property type="term" value="P:protein destabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07842">
        <property type="entry name" value="STKc_CDK8_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000408">
        <property type="entry name" value="Serine/threonine-protein kinase SSN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000426">
        <property type="entry name" value="Serine/threonine-protein kinase ssn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF495">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0010">Activator</keyword>
    <keyword evidence="13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="17" id="KW-0804">Transcription</keyword>
    <keyword evidence="15" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="26">
        <location>
            <begin position="87"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="419"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="25">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="25">
        <location>
            <begin position="23"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="25">
        <location>
            <begin position="53"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00039418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00040708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00041823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00049280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EMBL" id="CAZ81710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="453" mass="51632" checksum="5965247879D166E0" modified="2010-06-15" version="1">MQRAEDREVDDKDDRGGGRPGLLTSTSTIFNTITPTQHPPSKRKREGTHHEEGDEGEDEQEEDQEERGLHEEIMPPYKSKVRVLEEYHIIGFISSGTYGRVYKARSKLPGNTKEYAIKKFKPDKEGEIIQYTGISQSACREMALCSELSHENVIHLHEIILEDKCIYMVFEYAEHDLLQIVHYHSHPERRPIPEATIKSVLWQLLNGVSYLHQNWVLHRDLKPANIMVTAAGEVKIGDLGLARLFWKPLQSLYAGDKVVVTIWYRAPELLLGSKHYTAAIDLWAVGCIFAELLALRPIFKGEEAKMEKKSTVPFQRNQMQKIIEILGTPNKERWPAVVQQPEYNQLQAFKQYTNNLEAWYHQIGATNALGFQLLSGLLNYDPTQRLTAQSALEHPYFSEGAKLTLNAFEGQSFDYPHRRVSQDDNDISLPGTKRSGLPDDSLGGSRAKRLKEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>D5GB32</accession>
    <name>D5GB32_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Mitochondrial import inner membrane translocase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00005423001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Heterohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Mitochondrion inner membrane</location>
            <topology evidence="1 12">Peripheral membrane protein</topology>
            <orientation evidence="1 12">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="12">The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the small Tim family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430086">
        <property type="protein sequence ID" value="CAZ81725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837534.1">
        <property type="nucleotide sequence ID" value="XM_002837488.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GB32"/>
    <dbReference type="FunCoup" id="D5GB32">
        <property type="interactions" value="850"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GB32"/>
    <dbReference type="GeneID" id="9187966"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005423001"/>
    <dbReference type="eggNOG" id="KOG3479">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_141397_3_0_1"/>
    <dbReference type="InParanoid" id="D5GB32"/>
    <dbReference type="OMA" id="QDFLRMY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.810">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit tim13 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050673">
        <property type="entry name" value="Mito_inner_translocase_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004217">
        <property type="entry name" value="Tim10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035427">
        <property type="entry name" value="Tim10-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13172">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM9B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02953">
        <property type="entry name" value="zf-Tim10_DDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144122">
        <property type="entry name" value="Tim10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0143">Chaperone</keyword>
    <keyword evidence="10 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 12" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0811">Translocation</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Tim10-like" evidence="13">
        <location>
            <begin position="14"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU367043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ81725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="97" mass="10786" checksum="B6F9A79946FFBEE9" modified="2010-06-15" version="1">MDSLSPSEQREFQSVLEKRQTRDIMQFYSNLVQRCFDDCINDFTSKALSTKEETCVSRCVDKFVKTSDRLGQRFAEQNQAMMQGGGAGGAGAGFGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBA3</accession>
    <name>D5GBA3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00000537001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430089">
        <property type="protein sequence ID" value="CAZ81796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837605.1">
        <property type="nucleotide sequence ID" value="XM_002837559.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBA3"/>
    <dbReference type="STRING" id="656061.D5GBA3"/>
    <dbReference type="GeneID" id="9181507"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000537001"/>
    <dbReference type="HOGENOM" id="CLU_832066_0_0_1"/>
    <dbReference type="InParanoid" id="D5GBA3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072680" evidence="3">
        <location>
            <begin position="19"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="312"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="158"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="249"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="166"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="272"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="283"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ81796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="334" mass="33119" checksum="B6179E181E7BA775" modified="2010-06-15" version="1">MRPTSIILLGASVAVAAASGESQPKVKLINRRDAVPIPDVFMNAIQAVPALRKRELGPPTYYASAPEGVRKRSSLESRQWGCETVGQINCVFEGCCNVGTICGVYGGQTGCCPIGKTCTGVGSCKTGETICGSGCCPSNASCTSNADGSAACDYGTGSSSTRTLMRPTATSARLSSTSTSTSLPGSGSECPAGYSPCADTDTCCPAGVKCLPNKKCDSKCSVSDIKCGTGCCKGGFTCDTATFICQGNGSDSSDDSGDSGSGTSSGGTRSTVSFTRFTATANSGSSSGTTSRSSVPTGITASDGGANSGPRLIAGMGMAGIIAGVVAIVGVALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GC25</accession>
    <name>D5GC25_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00005787001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430098">
        <property type="protein sequence ID" value="CAZ82068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837877.1">
        <property type="nucleotide sequence ID" value="XM_002837831.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GC25"/>
    <dbReference type="GeneID" id="9181337"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005787001"/>
    <dbReference type="HOGENOM" id="CLU_2456394_0_0_1"/>
    <dbReference type="InParanoid" id="D5GC25"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ82068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="89" mass="9715" checksum="9B209D92B31EA54E" modified="2010-06-15" version="1">MVTDRCRYHGISFTSDLPLAQFGWLGDGRSSKGGNADWRLPPPPGDRSQFGLRCGKLGHSCRGYSSGRIFTCWCVLGKIDGLVSQVQPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5GC71</accession>
    <name>D5GC71_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">assimilatory sulfite reductase (NADPH)</fullName>
            <ecNumber evidence="4">1.8.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">GSTUM_00000610001</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">This enzyme catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>hydrogen sulfide + 3 NADP(+) + 3 H2O = sulfite + 3 NADPH + 4 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13801"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17359"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.8.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sulfur metabolism; hydrogen sulfide biosynthesis; hydrogen sulfide from sulfite (NADPH route): step 1/1.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.2" evidence="4"/>
    <dbReference type="EMBL" id="FN430103">
        <property type="protein sequence ID" value="CAZ82114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837923.1">
        <property type="nucleotide sequence ID" value="XM_002837877.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GC71"/>
    <dbReference type="FunCoup" id="D5GC71">
        <property type="interactions" value="374"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GC71"/>
    <dbReference type="GeneID" id="9184790"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000610001"/>
    <dbReference type="eggNOG" id="KOG1158">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003662_1_0_1"/>
    <dbReference type="InParanoid" id="D5GC71"/>
    <dbReference type="OMA" id="MIVAVNW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009337">
        <property type="term" value="C:sulfite reductase complex (NADPH)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004783">
        <property type="term" value="F:sulfite reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000103">
        <property type="term" value="P:sulfate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06207">
        <property type="entry name" value="CyPoR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.920:FF:000007">
        <property type="entry name" value="Pyruvate:ferredoxin (Flavodoxin) oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.990.10:FF:000010">
        <property type="entry name" value="Sulfite reductase [NADPH] flavoprotein component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000052">
        <property type="entry name" value="Sulfite reductase [NADPH] flavoprotein component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000011">
        <property type="entry name" value="Sulfite reductase flavoprotein component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.990.10">
        <property type="entry name" value="NADPH-cytochrome p450 Reductase, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003097">
        <property type="entry name" value="CysJ-like_FAD-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023173">
        <property type="entry name" value="NADPH_Cyt_P450_Rdtase_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002869">
        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384">
        <property type="entry name" value="NITRIC OXIDE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384:SF109">
        <property type="entry name" value="SULFITE REDUCTASE [NADPH] FLAVOPROTEIN COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00667">
        <property type="entry name" value="FAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53323">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="12" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10" id="KW-0274">FAD</keyword>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0288">FMN</keyword>
    <keyword evidence="14" id="KW-0408">Iron</keyword>
    <keyword evidence="15" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0521">NADP</keyword>
    <keyword evidence="13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FAD-binding FR-type" evidence="18">
        <location>
            <begin position="657"/>
            <end position="888"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00052219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00059320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAZ82114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1044" mass="114386" checksum="F587237BC774358C" modified="2010-06-15" version="1">MAHQSGVKNPLEEISPVFPHSVPADISSVNGTLYTTAQTLVQQVAYTLSDKLFTYSPDTFSLDEAARLWSGNEQTNVRGVVPTVNAMETRLGAANVLLGYVFSPDVDPKKKAVAKSIIASSPALIHMRSALDQLSVLYGVSSPFVAHVSAVDYEVSSAKLVTDYASAVTVAQETGIGLLASFSAHEAQHMALFATLAATVLPMVHIYDGVSVSRESAKIKDVLDRTSLQNVFSSILGEQKEAPKKADQGTKVNAILRGLNAELGTAYSLFEYEGHDEPDAVLVTFGSVESLLATQVAASLTNSGERIGVIAIRVYRPFSEPHFLEALPRSVKRIAVLGQVANKAAAEDSAVQSALYVDVVAAITMSDIWTLPPPIVDIPEEELTAQASTMDAFILLGDGSHAQQYVFWDGDDAASAPAASIISRLLSEDPRKSVSFQAAYDNVPLSGILHSEIRASSSAIDAPFHIECADISVVNDIQLLSKFDIASRTKDSGIILLKSTIKDDEFEKKLPALFRRAVTKKAINLIIADPSALGDEAPAAVENALIQLAFAKLANISAPLEKLSYLNIDSSTVHQAYERLDSSLRNVEIPKEWADSLEDNEPSHLPTIPQTTSFAINEEKHTTEPAPALKTSQSAAQAIVFKEAYDVQSALRPDLSMKNYIVKVQENRRLTPEYYGRNIFHIEFDLSGTGMEYQIGEALGIHSQNDPDEVSEFIEFYGLNADDIVEVPSKDDPDVLEMRTVFQALSQNVDIFGRPPKKFYESLAEFATDEGERKALLTLSSAEGATESKRRAEMDTVTFADILAEYKSAHPSFYELVRIVSPLKRREYSIASSQKVNPNSVHLLIVVVNWVDTKGRDRFGQATRYLSRLPIGAKVTVSVKPSVMKLPPLATQPLIMAGLGTGLAPFRAFVQYRAWQKSQGVDIGSVLLYMGSRHQREEYLYGEEWEAYQAAGIVTLLGRAFSRDQSEKIYIQDRMRQSLDDIIEAYIKEDGAFYLCGPTWPVPDVTEVLQEAIVEEGKRRGAKVDGAKKIEELKEGLRYVLEVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5GC82</accession>
    <name>D5GC82_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00000634001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430105">
        <property type="protein sequence ID" value="CAZ82125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837934.1">
        <property type="nucleotide sequence ID" value="XM_002837888.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GC82"/>
    <dbReference type="GeneID" id="9184803"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000634001"/>
    <dbReference type="HOGENOM" id="CLU_2122860_0_0_1"/>
    <dbReference type="InParanoid" id="D5GC82"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="114" mass="12652" checksum="7A243347CE3DEDDE" modified="2010-06-15" version="1">MHPGNPVQHDKAQPSPRHACHSHYSTNNPIKSLAEPALFFLHPFPFHPSILPSLPPSLLPSFLPPSKRRAIASVRDHSITPTKIRSVHPSPMLELMTPRQQPIFSLPINNLAIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>D5GC95</accession>
    <name>D5GC95_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="12">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00000585001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430106">
        <property type="protein sequence ID" value="CAZ82138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837947.1">
        <property type="nucleotide sequence ID" value="XM_002837901.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GC95"/>
    <dbReference type="FunCoup" id="D5GC95">
        <property type="interactions" value="7"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GC95"/>
    <dbReference type="GeneID" id="9184813"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000585001"/>
    <dbReference type="eggNOG" id="KOG0236">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003182_0_2_1"/>
    <dbReference type="InParanoid" id="D5GC95"/>
    <dbReference type="OMA" id="ENEDFWF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034618">
        <property type="term" value="F:arginine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015174">
        <property type="term" value="F:basic amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034490">
        <property type="term" value="P:basic amino acid transmembrane import into vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07042">
        <property type="entry name" value="STAS_SulP_like_sulfate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000141">
        <property type="entry name" value="Sulfate transporter family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.750.24:FF:000012">
        <property type="entry name" value="Sulfate transporter family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="entry name" value="STAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052706">
        <property type="entry name" value="Membrane-Transporter-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43310:SF4">
        <property type="entry name" value="AFR304WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43310">
        <property type="entry name" value="SULFATE TRANSPORTER YBAR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SpoIIaa-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="396"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="428"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="466"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="500"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="555"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="633"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="659"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="689"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="domain" description="STAS" evidence="11">
        <location>
            <begin position="757"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="10">
        <location>
            <begin position="975"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="110"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="150"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="151"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ82138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1091" mass="120375" checksum="6AE865790A947AF3" modified="2010-06-15" version="1">MSSSSYPRRGFTMPGVPLPLEDTLQREPDEETGLLQPHGPRNAQSDYILTSRSPPSPPTLLGKQNSRSFFHSSYHSHHLHDPDKVTWPQYTSSLGVRDQTAELATLALSDAGSVRGSPPPRPLSSSYPDIGLSPTVEGSLNRTRVIVEVAEPPSESETPTSIPGKSYLTHLLRNSPPETDSESETDAEAQQGLHATDYNPHSPVVDDLPAPNNTGGPPSTPKVHPSGDGTRLYDAIQQPNGEAVSHRGYGAVPGVKAIRVITWPTEKGLNAMKSMLHRRKMWRKQEIWENVVAKPVGCLPAVLLGLLLNVLDGLSYGMILFPLGQSIFSDLGPDGLSIFYVSCIVSQLVYSCGGSIFKGGIGSEMIEVVPFFHKMAFTILNQVGEDRPKAVIATTILSYSLSSILTGAVFFALGYLKLGSLTGFFPRHILVGCIGGVGWFLVATGLEVSARLDGNLNYNLEVLQLLVKPATLVLWTVPLFLAIILIIIQRFIKHPLIVPLYFLSIPAVFYLVVLAVPNLNIASLRDQGWVFELPQAGVPFYHFYTLYGHEVNWKALASTVPAMFALTFFGILHVPINVPALGVSTGEDNVDVNRELVAHGISNAMSGFAGSIQNYLVYTNSILFIRSGGDSRLAGIMLAIGTFGIMAIGPTIIGYIPVLVVGALIFLLGIELLREALYDTWGKLSRLEYLTICTIVVTMGAWDFVIGILIGIVLACMSYVVQTSQKPAIRAIYSGAIARSTVRRHPNQQRFLRKVGPQIQVFKLAGYMFFGTIASVEGKIRSLLEEKNFQEQPIRFLIVDLLHVNGMDFSAAEAFTRMRRLLSAKNVEMILSSVQSESEVGRELRAVGVWGDNDKIQVFQDLNAALEACENEFLTAFYFHSDELATRKHNPPTHLDVPNRNPSDVHGIEAAYSSPRRNLLKLAAASTLRESESESQHSKWKNFKQPLPLILQTFQEMTDKHEDFWFRAIPFFVRREYAQGSIVFSRGDKSKEFYLLEEGMFRAEYDLEQGKYFESIVAGTTCGELPFFSATKRTATVTTEKKTVAWVLNKKSWESMQKIQPDIAKELLEISLKLTSERMSAITSYVLTSAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCA1</accession>
    <name>D5GCA1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Alkyl transferase</fullName>
            <ecNumber evidence="12">2.5.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00000645001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">With NUS1, forms the dehydrodolichyl diphosphate synthase (DDS) complex, an essential component of the dolichol monophosphate (Dol-P) biosynthetic machinery. Adds multiple copies of isopentenyl pyrophosphate (IPP) to farnesyl pyrophosphate (FPP) to produce dehydrodolichyl diphosphate (Dedol-PP), a precursor of dolichol which is utilized as a sugar carrier in protein glycosylation in the endoplasmic reticulum (ER).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>n isopentenyl diphosphate + (2E,6E)-farnesyl diphosphate = a di-trans,poly-cis-polyprenyl diphosphate + n diphosphate</text>
            <dbReference type="Rhea" id="RHEA:53008"/>
            <dbReference type="Rhea" id="RHEA-COMP:19494"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="ChEBI" id="CHEBI:136960"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
            <dbReference type="EC" id="2.5.1.87"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Forms an active dehydrodolichyl diphosphate synthase complex with NUS1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the UPP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.-" evidence="12"/>
    <dbReference type="EMBL" id="FN430108">
        <property type="protein sequence ID" value="CAZ82144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837953.1">
        <property type="nucleotide sequence ID" value="XM_002837907.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCA1"/>
    <dbReference type="FunCoup" id="D5GCA1">
        <property type="interactions" value="390"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GCA1"/>
    <dbReference type="GeneID" id="9184822"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000645001"/>
    <dbReference type="eggNOG" id="KOG1602">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038505_0_4_1"/>
    <dbReference type="InParanoid" id="D5GCA1"/>
    <dbReference type="OMA" id="FDRRDLW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904423">
        <property type="term" value="C:dehydrodolichyl diphosphate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045547">
        <property type="term" value="F:ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyl diphosphate specific] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043048">
        <property type="term" value="P:dolichyl monophosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016094">
        <property type="term" value="P:polyprenol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00475">
        <property type="entry name" value="Cis_IPPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1180.10:FF:000002">
        <property type="entry name" value="Alkyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1180.10">
        <property type="entry name" value="Decaprenyl diphosphate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01139">
        <property type="entry name" value="ISPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001441">
        <property type="entry name" value="UPP_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018520">
        <property type="entry name" value="UPP_synth-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036424">
        <property type="entry name" value="UPP_synth-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00055">
        <property type="entry name" value="uppS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291:SF43">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE COMPLEX SUBUNIT DHDDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01255">
        <property type="entry name" value="Prenyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64005">
        <property type="entry name" value="Undecaprenyl diphosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01066">
        <property type="entry name" value="UPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00058504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00064670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ82144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="267" mass="30246" checksum="80170810E0817CF8" modified="2010-06-15" version="1">MMQLQELLVNALRQGSVPQHVAFVMDGNRRFARSNHIETVEGHNMGFEALAKALCYKAGVKVVTIYAFSIENFKRPLHEVNALMEIAKIKLSQLCQHGELMDRYGASLRILGHRSLLRQDVLEAIEQATEMTKHNDKAILNVCFPYTSRDEMTTAIRDITATPGLMDIESITEKTITQHMFTSDCPPLDLLVRTSGVERLSDFMMWQCHQDTDIVFSDCLWPQFDIWKFLPILINWGVKRRKLEKGRGDVEVRGVGMGMGVGKGGGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCP0</accession>
    <name>D5GCP0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00000783001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the cytochrome b5 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430119">
        <property type="protein sequence ID" value="CAZ82283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838092.1">
        <property type="nucleotide sequence ID" value="XM_002838046.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCP0"/>
    <dbReference type="STRING" id="656061.D5GCP0"/>
    <dbReference type="GeneID" id="9187901"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000783001"/>
    <dbReference type="eggNOG" id="KOG0536">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_602953_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCP0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004128">
        <property type="term" value="F:cytochrome-b5 reductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.120.10:FF:000001">
        <property type="entry name" value="Cytochrome b5 reductase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051872">
        <property type="entry name" value="Cytochrome_b5/Flavoprotein_Rdt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46237:SF1">
        <property type="entry name" value="CYTOCHROME B5 REDUCTASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46237">
        <property type="entry name" value="CYTOCHROME B5 REDUCTASE 4 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0349">Heme</keyword>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
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        <location>
            <begin position="24"/>
            <end position="454"/>
        </location>
    </feature>
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        <location>
            <begin position="358"/>
            <end position="435"/>
        </location>
    </feature>
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        <location>
            <begin position="74"/>
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        </location>
    </feature>
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            <begin position="305"/>
            <end position="329"/>
        </location>
    </feature>
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        <location>
            <begin position="87"/>
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        </location>
    </feature>
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            <begin position="100"/>
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        </location>
    </feature>
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        </location>
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        </location>
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        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU362121"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="454" mass="47247" checksum="07F20660CCD82E89" modified="2010-06-15" version="1">MIVGLSLILAFLSYLLYRRLSSADGTSGQSKALPMWPFFSAFIKQPLSPKPPSSPQTQATTAAAAVSITVQDYDAPNFEPAPKGKDTQQQQQQTRRTPAQAPPPPPPSSSPLSSPGTIPSLPPSLPNPHSPEATPKALAPSAAPTVGISAIPQISLLTDGERDGDDPGARGVESNNHNDGEQAEGDETDDDDTPPSFPSLNSAQRASGGISSLTASLSPSSSLSPSTNTSTMPSPITSTATTTLPMAPPPIPIPIPIRGRNPKANARLVLPGVPPQSSAAQQRQSESSAARSRLAAVGLGASGVAAGNGLTLPNQRKSATPSSSSRKKVILEPGHSPLDWARLQRSGVDLRGLSHSNLIKVSPSMLATHAKAPDDIWTALNGRVYNITAYLPFHPGGEKDLLRGAGKDCTKLFNATHPWVNVEGMLAECLIGIYITEEEAVALAAPESSLESVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCU2</accession>
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        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
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        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
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        <lineage>
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            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
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            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
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    </dbReference>
    <dbReference type="RefSeq" id="XP_002838144.1">
        <property type="nucleotide sequence ID" value="XM_002838098.1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
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            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
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        </location>
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            <begin position="52"/>
            <end position="116"/>
        </location>
    </feature>
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        <location>
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    </feature>
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    </feature>
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    </feature>
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            <begin position="139"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ82335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="148" mass="15700" checksum="E2ECF48758602173" modified="2010-06-15" version="1">MSVPPPDPAGTLPRTTGPLKNRLPIYIGAGVATIAAYYFYNAGGDASVARKKMKQDASRASASIRDELPHRGTENKTSAEESMAKAGSAIDSTVRDGKSRITEGQHKLEETKDKTGKAIMSKIDEADLKIESESSKAKGGIGSWFGSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
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    <name>D5GDC4_TUBMM</name>
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        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00006147001</name>
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        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
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        <lineage>
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            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
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            <taxon>Tuber</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
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                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
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                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
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                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
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                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
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                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
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                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CWC21 family.</text>
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    <dbReference type="EMBL" id="FN430142">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002838280.1"/>
    </dbReference>
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    <dbReference type="FunCoup" id="D5GDC4">
        <property type="interactions" value="45"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GDC4"/>
    <dbReference type="GeneID" id="9183981"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006147001"/>
    <dbReference type="eggNOG" id="KOG1869">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067891_2_1_1"/>
    <dbReference type="InParanoid" id="D5GDC4"/>
    <dbReference type="OMA" id="LAHMRPR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21372">
        <property type="entry name" value="cwf21_CWC21-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051372">
        <property type="entry name" value="CWC21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013170">
        <property type="entry name" value="mRNA_splic_Cwf21_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36562">
        <property type="entry name" value="SERINE/ARGININE REPETITIVE MATRIX 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36562:SF5">
        <property type="entry name" value="SERINE_ARGININE REPETITIVE MATRIX 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08312">
        <property type="entry name" value="cwf21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01115">
        <property type="entry name" value="cwf21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="CWF21" evidence="8">
        <location>
            <begin position="57"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="130"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
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            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="133"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ82517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="169" mass="19363" checksum="A73AA774B8218C27" modified="2010-06-15" version="1">MSSNVGLSTPRGSGTSGYVQRNLSHLRPRDYPPPSSSSAIASNTREYRQRQPDKEILEHDRKRGVEVKCLEFRDKLEDEGVDEDDIDERVETYRKKLLGELEREGEGWDVHEIAVAKAAESERLRNALGISKDYQEGSHWKKQEDERQKRLGDREKEVAASRRRSASPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDD1</accession>
    <name>D5GDD1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00006157001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family. Azg-like subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430142">
        <property type="protein sequence ID" value="CAZ82524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838333.1">
        <property type="nucleotide sequence ID" value="XM_002838287.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDD1"/>
    <dbReference type="STRING" id="656061.D5GDD1"/>
    <dbReference type="GeneID" id="9183987"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006157001"/>
    <dbReference type="eggNOG" id="ENOG502QQ5E">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1120804_0_0_1"/>
    <dbReference type="InParanoid" id="D5GDD1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005345">
        <property type="term" value="F:purine nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015853">
        <property type="term" value="P:adenine transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015854">
        <property type="term" value="P:guanine transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045018">
        <property type="entry name" value="Azg-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006043">
        <property type="entry name" value="NCS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43337">
        <property type="entry name" value="XANTHINE/URACIL PERMEASE C887.17-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43337:SF1">
        <property type="entry name" value="XANTHINE_URACIL PERMEASE C887.17-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00860">
        <property type="entry name" value="Xan_ur_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="113"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="167"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="197"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ82524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="248" mass="26353" checksum="D832DBB272B2950A" modified="2010-06-15" version="1">MARFAGFIDERTQDFEGSAMAYIVDAMGISLGSLFGSPPVTAYIESGAGISEGGITGITAMVTGVCFFISVFFAPIFASIPPWATGSTLIIVGSMMMKAAADINWSYPGDGIPAFVTIMVMPFTYSIAYGLIAGIITYIILNTGAWFIEIASGGRIVPYGKELKDHWTWRIPGGFFPAWVVRIAHGDRKFWKREAYDGQSGGASGASTPQEKVGREPRGAASEDLGVEMGSAGSRVAVGKDEKFVKDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDL6</accession>
    <name>D5GDL6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00001078001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430150">
        <property type="protein sequence ID" value="CAZ82609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838418.1">
        <property type="nucleotide sequence ID" value="XM_002838372.1"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GDL6"/>
    <dbReference type="GeneID" id="9181621"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001078001"/>
    <dbReference type="eggNOG" id="KOG0101">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009958_6_1_1"/>
    <dbReference type="InParanoid" id="D5GDL6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10170">
        <property type="entry name" value="ASKHA_NBD_HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14187">
        <property type="entry name" value="ALPHA KINASE/ELONGATION FACTOR 2 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14187:SF82">
        <property type="entry name" value="FAMILY CHAPERONE, PUTATIVE (AFU_ORTHOLOGUE AFUA_7G08575)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ82609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="667" mass="73403" checksum="0A568EC82A4AE45D" modified="2010-06-15" version="1">MSSSKQRLIIGLDYGTTYTGVAFCDSSAGGSIEGIQLIHNWPGKSQNATNEKVPSQVAYGSFADGRTYEWGNRIPPRAERQVWTKLQLDEGQKREELKMLLALLSGNLENMSIGGSGEDDDNPPLYPGKQPQDVVADFLTGVNEHVVQTLIKQFSHRLLSTLQIEIVITVPAVWSDRAKDLTFQAVGKAGLGEFKYTTSMIAEPEAAAIYTLKSLKGGASGDDIQVGDHFVLCDAGGGTVDLISYRVSSVSPSFKVEEAAVGTGDKCGATFVDRNFKEWLKGKLGEANYKKIPKEKLMIGSKLMKEFEDAKTSFSGDGPEGLITVPSEVGITEDASRGIEDGEILITAADFQEIFDPCINRTLELIDGQIAAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSMASGNYPFKKSLLSPIAVVQSRGSRYVFLVGGFGKSEYMFKKVQEYCVARGLETRRPPFPWSAVVRGAVARGLEGEGSGLVQLRMCRRHYGTPVSEHFDETKHTVQDMYVDDLTGERFAKGQMHWLLTKGESLSATTPKRVSIECCRTFRPTDSRVFSAKLVACNNDYAPRRYFENSVYNVCTLHADISTVPESKFHQARRGLGGEMFYIAEFKIEIIAHGANLKFFLTFEGQEYGSVSANYDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEP3</accession>
    <name>D5GEP3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00001304001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430181">
        <property type="protein sequence ID" value="CAZ82986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838795.1">
        <property type="nucleotide sequence ID" value="XM_002838749.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEP3"/>
    <dbReference type="FunCoup" id="D5GEP3">
        <property type="interactions" value="33"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GEP3"/>
    <dbReference type="GeneID" id="9184897"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001304001"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_11_5_1"/>
    <dbReference type="InParanoid" id="D5GEP3"/>
    <dbReference type="OMA" id="RWDPTRT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF51">
        <property type="entry name" value="ALPHA-GLUCOSIDE TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G11920)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="53"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="156"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="294"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="329"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="357"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="391"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="423"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="457"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="56"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ82986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="511" mass="55714" checksum="91B2EF9E12F95253" modified="2010-06-15" version="1">MDANPASLSGEDVKGASSGQLDLDVTQHTLFEEANRYDHNQTFREALRDEKGVLLWAGVMGAAALSWGFDAQVNGAAISVEAFRRDFGYIYEGKAVLPANWQTIFNVTSTVGAFFGGLASGYIADRVGRKWCAWLGSWILCAGIALEIAATTRAVFTVAKVILGVALGFYLSVAPAYCSEISPVVLRGITTCGVSLAIVVGQLLSNVAIKIFGERLDRWAYRGPFCVHPHGTWYARVTSRELALLSFGILHGTDRDIAPRLAQIQETLRLENELYTNAAWLDCFKGVDARRTMITVGVFCCQHLSGIIFVLGYSTYFFQLAGLPTSQTFSLGVGVTACGVAGNISSWWLVNTVGRRIVAFYGMVVLTGMLTLIGVFDVIPTAGAQWAQSAFTVIYSYVWFLTIGAMGYVILGEASSSRLRSKSVGLAAVVQSLWGLIMNFALPYFVNPDEANLRGKVGFIFGGTSLLATVWVFFCVPEFKGLTFSEIDERFKMRVPARKFQEYKGRPESDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>D5GF55</accession>
    <name>D5GF55_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00001862001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430207">
        <property type="protein sequence ID" value="CAZ83148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838957.1">
        <property type="nucleotide sequence ID" value="XM_002838911.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GF55"/>
    <dbReference type="GeneID" id="9188009"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001862001"/>
    <dbReference type="eggNOG" id="ENOG502SQZN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_669383_0_0_1"/>
    <dbReference type="InParanoid" id="D5GF55"/>
    <dbReference type="OMA" id="CGTSTHE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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        </location>
    </feature>
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        <location>
            <begin position="249"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="96"/>
        </location>
    </feature>
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        </location>
    </feature>
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            <end position="261"/>
        </location>
    </feature>
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        <location>
            <begin position="262"/>
            <end position="285"/>
        </location>
    </feature>
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        <location>
            <begin position="300"/>
            <end position="314"/>
        </location>
    </feature>
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        <location>
            <begin position="315"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="332"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="411" mass="44337" checksum="F3D43A6F789570E6" modified="2010-06-15" version="1">MTPPSFSSSMPGGSFLPSIPNSIPAYPKTVPAHAFRHPNQSPSPTFGPTRKRLQRDSGFSAQSPPPFEDAGDSDEDVMRNSLYSDSAYRSTLATSNSRKRGLADDDADDYEHGPGARSGDGAGDSNRPPKRRIIDVVGSVASRVWEFCKARVPIYGPTSRMTAAEVDMNADEHLYDPGMPSYLMNGAQFGTGLYDNQHFIASSPPQPTNAGAGGMSGEEQSYFSSRDFSPNKQVDGGLSSRWVMVSPAPTASTAPQQHNNKPSFSASASSSRTSTPTHSRRPQSSASSKRTNRPIIASSARKRPTVVRPSHQRSRSSSTVTSSTFAPPPPQSAFTFHSSPISGGASVANTRGRRKGRIPGLAATPSLGDDEEDESMRRFNERLRDMIREGREALGSKVEVVYDEDDDLENF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFC9</accession>
    <name>D5GFC9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">Vacuolar fusion protein MON1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00006828001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">In complex with CCZ1, is required for multiple vacuole delivery pathways including the cytoplasm to vacuole transport (Cvt), autophagy, pexophagy and endocytosis. The MON1-CCZ1 complex acts at the fusion of vesicles with the vacuole, through its regulation of the SNARE complex during the coordinated priming and docking stages of fusion, and particularly at the stage of tethering/docking.</text>
    </comment>
    <comment type="function">
        <text evidence="4">Required for multiple vacuole delivery pathways including the cytoplasm to vacuole transport (Cvt), autophagy, pexophagy and endocytosis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endosome</location>
            <location evidence="4">Multivesicular body membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 4">Prevacuolar compartment membrane</location>
            <topology evidence="1 4">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Vacuole membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the MON1/SAND family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430208">
        <property type="protein sequence ID" value="CAZ83222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839031.1">
        <property type="nucleotide sequence ID" value="XM_002838985.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFC9"/>
    <dbReference type="FunCoup" id="D5GFC9">
        <property type="interactions" value="529"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GFC9"/>
    <dbReference type="GeneID" id="9188078"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006828001"/>
    <dbReference type="eggNOG" id="KOG0997">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014574_5_0_1"/>
    <dbReference type="InParanoid" id="D5GFC9"/>
    <dbReference type="OMA" id="QQPFNAK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035658">
        <property type="term" value="C:Mon1-Ccz1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032585">
        <property type="term" value="C:multivesicular body membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043972">
        <property type="entry name" value="FUZ/MON1/HPS1_longin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043971">
        <property type="entry name" value="FUZ/MON1/HPS1_longin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043970">
        <property type="entry name" value="FUZ/MON1/HPS1_longin_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004353">
        <property type="entry name" value="Mon1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13027">
        <property type="entry name" value="SAND PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13027:SF7">
        <property type="entry name" value="VACUOLAR FUSION PROTEIN MON1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19036">
        <property type="entry name" value="Fuz_longin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19037">
        <property type="entry name" value="Fuz_longin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19038">
        <property type="entry name" value="Fuz_longin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01546">
        <property type="entry name" value="YEAST73DUF"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="4" id="KW-0926">Vacuole</keyword>
    <feature type="domain" description="FUZ/MON1/HPS1 first Longin" evidence="6">
        <location>
            <begin position="204"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="FUZ/MON1/HPS1 second Longin" evidence="7">
        <location>
            <begin position="366"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="FUZ/MON1/HPS1 third Longin" evidence="8">
        <location>
            <begin position="495"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="25"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF19036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF19037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF19038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ83222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="604" mass="65930" checksum="6A3E23885B0C78B8" modified="2010-06-15" version="1">MSTTSPPPSPPCSHPSPSTSTSSSNEDQLPTPRASTRPTAITLTEDYFAPPLPPRPSLEVIASSTLLSIQSTTSSGASTPGLQAKPTTAISLPEGVHAQQQDAVVSGNGSITGAPSFSDSVSVKSLVPTLSAGDNDVESMLGEILDEEGEGGGFLKARRDIMNIGGGGAGIEEVEFEDEGWDESSDEEGLGEEELVAKWRAKRKHFFILSSAGKPIYSRHGDESVLSGYMGVIQAIISFFQDDNDTLKSFSAGRHRFAIAASGPLYLVAISSMGESDSQLRAQLDALYTQVLSTLTLSQLNKIFAQRGNFDLRRLLGGTEVFLDGLSDAMTLGSPQVLLSALECVKMRKSHREKINSILLKHRSPNLLYGLVIADGRMVSVLRPKRHSLHPPDLQLLFSMLFNATTFRDGGEHWTPICLPKFNSKGFLHAYIHFFRKEVAVVLISADKDAFFEMRVMKEVIVEQLEKAGSIKIIEEAVGNGRYTTTDVLSSETAIRHFLYRSKNNVQFTMPSFEPHFYSAIARRRLMNLYQRLHAAVHAKNALLKVHHCIRNSSVSLSWVTPTFELYCVAVAGANRAALAKSATAIAAWVKREEERLFIGGVIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFE5</accession>
    <name>D5GFE5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Phosphotransferase</fullName>
            <ecNumber evidence="11">2.7.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">GSTUM_00006856001</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>D-fructose + ATP = D-fructose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16125"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37721"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:16126"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22740"/>
            <dbReference type="ChEBI" id="CHEBI:4194"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:229467"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:22741"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate metabolism; hexose metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the hexokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.-" evidence="11"/>
    <dbReference type="EMBL" id="FN430208">
        <property type="protein sequence ID" value="CAZ83238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839047.1">
        <property type="nucleotide sequence ID" value="XM_002839001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFE5"/>
    <dbReference type="FunCoup" id="D5GFE5">
        <property type="interactions" value="796"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GFE5"/>
    <dbReference type="GeneID" id="9188186"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006856001"/>
    <dbReference type="eggNOG" id="KOG1369">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014393_5_2_1"/>
    <dbReference type="InParanoid" id="D5GFE5"/>
    <dbReference type="OMA" id="ADCVQQF"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00180"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005536">
        <property type="term" value="F:D-glucose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008865">
        <property type="term" value="F:fructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004340">
        <property type="term" value="F:glucokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019158">
        <property type="term" value="F:mannokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001678">
        <property type="term" value="P:intracellular glucose homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006013">
        <property type="term" value="P:mannose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000092">
        <property type="entry name" value="Phosphotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.367.20:FF:000004">
        <property type="entry name" value="Phosphotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.367.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001312">
        <property type="entry name" value="Hexokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019807">
        <property type="entry name" value="Hexokinase_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022673">
        <property type="entry name" value="Hexokinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022672">
        <property type="entry name" value="Hexokinase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443">
        <property type="entry name" value="HEXOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443:SF16">
        <property type="entry name" value="HEXOKINASE TYPE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00349">
        <property type="entry name" value="Hexokinase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03727">
        <property type="entry name" value="Hexokinase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00475">
        <property type="entry name" value="HEXOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00378">
        <property type="entry name" value="HEXOKINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51748">
        <property type="entry name" value="HEXOKINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 11" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="6 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Hexokinase N-terminal" evidence="13">
        <location>
            <begin position="27"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="Hexokinase C-terminal" evidence="14">
        <location>
            <begin position="230"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF03727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAZ83238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="497" mass="55265" checksum="5DBB9A0117D423DD" modified="2010-06-15" version="1">MYLSASRKPPSRKGSMHDVPNDLMKHIHELEKMFTIDTPKLHEIVSVFQEELIKGLSVAGGTIPMNPTWVIGYPTGDETGTYLALDMGGTNLRVCEVELPEEKGQYEICQSKYRLPEEIKSGTGEQLFDYIAECVKQFLVANHDGQDVKDLKELHLGFTFSYPCEQNAIDHGILQRWTKGFDIEGVEGHDVVPMFEAALERKGVPIKITALVNDTTGTLIASAYTDNTTRIGCIFGTGCNAAYMETVGSIPKLAHMNLDPKLDIAINCEWGAFDNEHRVLPRTVYDKHIDEHSPRPGQQTFEKMVAGLYLGEIFRLVLVDLHSNPDVKIFEKQDISALQTEYSLDSSFLAEIESDPWENLMETHALFEKKLKIETTEPERKLIRRLSELIGTRAARLSACGVAAICKMKNIESCHVGADGSVFNKYPNFQERGAKALREIFGWGRAVRKDPIQIVPSEDGSGVGAALIAALTMKRIRGGIHAGVNVKRKNSLIPPVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFN6</accession>
    <name>D5GFN6_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">glucan 1,3-beta-glucosidase</fullName>
            <ecNumber evidence="14">3.2.1.58</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Exo-1,3-beta-glucanase D</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">GSTUM_00006999001</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Glucosidase involved in the degradation of cellulosic biomass. Active on lichenan.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Successive hydrolysis of beta-D-glucose units from the non-reducing ends of (1-&gt;3)-beta-D-glucans, releasing alpha-glucose.</text>
            <dbReference type="EC" id="3.2.1.58"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.58" evidence="14"/>
    <dbReference type="EMBL" id="FN430209">
        <property type="protein sequence ID" value="CAZ83329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839138.1">
        <property type="nucleotide sequence ID" value="XM_002839092.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFN6"/>
    <dbReference type="STRING" id="656061.D5GFN6"/>
    <dbReference type="GeneID" id="9188277"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006999001"/>
    <dbReference type="eggNOG" id="ENOG502QRG8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004624_4_2_1"/>
    <dbReference type="InParanoid" id="D5GFN6"/>
    <dbReference type="OMA" id="TYHEGFL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000935">
        <property type="term" value="C:division septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990819">
        <property type="term" value="C:mating projection actin fusion focus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031520">
        <property type="term" value="C:plasma membrane of cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004338">
        <property type="term" value="F:glucan exo-1,3-beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904541">
        <property type="term" value="P:fungal-type cell wall disassembly involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009251">
        <property type="term" value="P:glucan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000033">
        <property type="entry name" value="Glucan 1,3-beta-glucosidase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050386">
        <property type="entry name" value="Glycosyl_hydrolase_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31297:SF34">
        <property type="entry name" value="GLUCAN 1,3-BETA-GLUCOSIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31297">
        <property type="entry name" value="GLUCAN ENDO-1,6-BETA-GLUCOSIDASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 16" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="18">
        <location>
            <begin position="78"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00036824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00041260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU361153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAZ83329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="446" mass="49831" checksum="5FE5F6B4DCDE874E" modified="2010-06-15" version="1">MGLWDDYDDSVQANENVPPLNQEFPYGTKPIRGVNLGGWLIVEPFITPSFFNKIDSRFGVVDEFTLSKHLGPTNAAKTIEQHYANFINESAFREVRDAGLDHVRIPFGYWAIMTLGGDPFVPMISWRYLLRAIEYARKYGLRVKLDLHSVPGGANGWNHSGKLGPIGWLNGTDGDTNAQKTLDIHNQMATFFSQPRYKNVVTMYGLVNEPKMIFLDADKVIEWTVKAHGVVRKAGYQGYIIFGDGFRGLDNWKGEFKSLDKMLLDVHQYVIFNNVQISATHSAKVKFACDGWGQQMARSVDTSTGFGPTMVGEWGQAETDCTPYLNNVGIGSRWEGTLNSGDPLTQTDTPSCPGGKGCSCEGANANPSDYSKSYKKFLLMFAEAQMDSFEKCWGWLYWTWDTEAATQWSYKKGLAAGILPKKAYTRSFKCGDTIPDFGSEGLPETY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFP7</accession>
    <name>D5GFP7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="11">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00001911001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the steroid 5-alpha reductase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430218">
        <property type="protein sequence ID" value="CAZ83340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839149.1">
        <property type="nucleotide sequence ID" value="XM_002839103.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFP7"/>
    <dbReference type="FunCoup" id="D5GFP7">
        <property type="interactions" value="371"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GFP7"/>
    <dbReference type="GeneID" id="9188290"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001911001"/>
    <dbReference type="eggNOG" id="KOG1639">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059260_0_1_1"/>
    <dbReference type="InParanoid" id="D5GFP7"/>
    <dbReference type="OMA" id="ATMPIFN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001104">
        <property type="entry name" value="3-oxo-5_a-steroid_4-DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039357">
        <property type="entry name" value="SRD5A/TECR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10556">
        <property type="entry name" value="3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10556:SF28">
        <property type="entry name" value="VERY-LONG-CHAIN ENOYL-COA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02544">
        <property type="entry name" value="Steroid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50244">
        <property type="entry name" value="S5A_REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="293"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="3-oxo-5-alpha-steroid 4-dehydrogenase C-terminal" evidence="10">
        <location>
            <begin position="176"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ83340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="336" mass="37960" checksum="D832775C6A741641" modified="2010-06-15" version="1">MISLQLKPRGRSIKKLPSTIELDLATATAETLYTRLSAHTGLSRDRLRLTKGSDGSAVTVKRESGKDIMVEELGLRDGSTVFVKDLGPQVAWRTVFMIEYLGPLFIHPLLLYIVRPYIYSSSPLNPLSYIPYGPSLKPDQDLPPPTQTQALLCALVVLHFLKREYETFFIHRFSGTMPFRYIFRNSAHYWILSGVNLAYSLYTPAASPAASWWPAALNADNNPAVVYGMVALWVFAQISNFKTHLTLRNLRPANSTKRQIPQGYGFDSVTCPNYSFEVLSWVCVWVLSGGNWAASLFTLTGTTVMFLWAQKKEKRYRKEFGSTYKKKRNVMVPGIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFS2</accession>
    <name>D5GFS2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007048001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430220">
        <property type="protein sequence ID" value="CAZ83365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839174.1">
        <property type="nucleotide sequence ID" value="XM_002839128.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFS2"/>
    <dbReference type="STRING" id="656061.D5GFS2"/>
    <dbReference type="GeneID" id="9187271"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007048001"/>
    <dbReference type="eggNOG" id="ENOG502SU8Q">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1595759_0_0_1"/>
    <dbReference type="InParanoid" id="D5GFS2"/>
    <dbReference type="OMA" id="WGDAHPR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.970.10">
        <property type="entry name" value="Ribonuclease H1, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009027">
        <property type="entry name" value="Ribosomal_bL9/RNase_H1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011320">
        <property type="entry name" value="RNase_H1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037056">
        <property type="entry name" value="RNase_H1_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01693">
        <property type="entry name" value="Cauli_VI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55658">
        <property type="entry name" value="L9 N-domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribonuclease H1 N-terminal" evidence="1">
        <location>
            <begin position="6"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="Ribonuclease H1 N-terminal" evidence="1">
        <location>
            <begin position="90"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="167" mass="18528" checksum="7270F59DEE7CB52D" modified="2010-06-15" version="1">MARRGYYAVARGKPPAPAIYSSWAAAQVVTQKYPDVIFKKFSTFEEAQAFMAGHGVARYRVEITFDVTCMMEESRSPTSGVGGGTGGKQFYVVANGSGKGVYESWEQAKVHIFGQPGICHQAFGTRQEADEWLWEYGRMQDEVEYRANAKQGLGNLSDLARELPEPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFZ3</accession>
    <name>D5GFZ3_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protoheme IX farnesyltransferase, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Heme O synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">GSTUM_00001943001</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Converts protoheme IX and farnesyl diphosphate to heme O.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UbiA prenyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430229">
        <property type="protein sequence ID" value="CAZ83436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839245.1">
        <property type="nucleotide sequence ID" value="XM_002839199.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFZ3"/>
    <dbReference type="FunCoup" id="D5GFZ3">
        <property type="interactions" value="768"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GFZ3"/>
    <dbReference type="GeneID" id="9181781"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001943001"/>
    <dbReference type="eggNOG" id="KOG1380">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029631_2_0_1"/>
    <dbReference type="InParanoid" id="D5GFZ3"/>
    <dbReference type="OMA" id="TSAYGMY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008495">
        <property type="term" value="F:protoheme IX farnesyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006784">
        <property type="term" value="P:heme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd13957">
        <property type="entry name" value="PT_UbiA_Cox10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.140:FF:000004">
        <property type="entry name" value="Protoheme IX farnesyltransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00154">
        <property type="entry name" value="CyoE_CtaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006369">
        <property type="entry name" value="Protohaem_IX_farnesylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030470">
        <property type="entry name" value="UbiA_prenylTrfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01473">
        <property type="entry name" value="cyoE_ctaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43448">
        <property type="entry name" value="PROTOHEME IX FARNESYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43448:SF2">
        <property type="entry name" value="PROTOHEME IX FARNESYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00943">
        <property type="entry name" value="UBIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="193"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="222"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="267"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="294"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="318"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="359"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="400"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="100"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAZ83436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="500" mass="54569" checksum="F10B113D86CD73AE" modified="2010-06-15" version="1">MYSPNAVASPLALPRPLTNRNSSASNAMLLLLPQVLPPPTICSHCLRRLTTYTRRPFSSTLHPLRSLDAVSLHVPSLLPRSRPKWHSQYFFSNGYLRADAGDSGKHTHTRDGSTTPMREPALLPHRRRQRQQKLCEGVLSPHTTTAGANQSTAVTAMQLPPDASSLLSTTSSQQTSSWKRALYSYLALTKPRLTALIVLSAMAPYALFPVDPLLSDVPTLSALTLTFLTVGTALSSSSANAFNMYLEPTYDRQMSRTRNRPLVRGLLTPRQALVFAAATGLLGVGSLYLGVNPAVAALGGINIVLYAGVYTPLKRISVVNTWVGAIVGGIPPLMGWAAAAGNVQGVDSTWSSTLSHSGGWLLAGLLFAWQFPHFNSLSWGIRDEYRRVGYQMMVWKYPALNARVALRYSLLLFPICFGLWATGVTDVGFLVDSSAVNGWLVREAWAFYSKGGEGGNAKRLFWASVWHLPVVMGLAMVHKEGLWRGVWESIVGGAEEEEHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGB9</accession>
    <name>D5GGB9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00002008001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430254">
        <property type="protein sequence ID" value="CAZ83562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839371.1">
        <property type="nucleotide sequence ID" value="XM_002839325.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGB9"/>
    <dbReference type="STRING" id="656061.D5GGB9"/>
    <dbReference type="GeneID" id="9183160"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002008001"/>
    <dbReference type="eggNOG" id="KOG4668">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067138_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGB9"/>
    <dbReference type="OMA" id="IMIATEF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003954">
        <property type="term" value="F:NADH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015990">
        <property type="term" value="P:electron transport coupled proton transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.2700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003945">
        <property type="entry name" value="NU5C-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42829">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42829:SF2">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="18"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="93"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="201"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="305"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="NADH:quinone oxidoreductase/Mrp antiporter transmembrane" evidence="6">
        <location>
            <begin position="2"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ83562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="324" mass="36263" checksum="0BD2A679FA978CBB" modified="2010-06-15" version="1">MMVIAVGLSSYNVALFHLINHAFFVEALLFLGAGAVIHAVSDNQDQIKYGGLRVFLPLSYSVMLIASLSLVAFPFMTGFYSKDFILESAFGQFYFSSIVVYFIATIGAIFTTLYSVKVLYLTFLANPHGPLINYKQAHEGDIFISLPLITLAIFSIFFGYITKDIYIGLGSSFFADNSLFIHPSHEIMIATEFAVLTIFKLLPFISTIFFSVLALVLSEFFPNLLLAFKFTRFGYNIWGFFNQRFLVELFYNKYIIAFVLKLGGQTTKIIDKGGIELIGPFGLEKGLLNLSKNIAKLDTGIITSYALYILIGLIIYILIYTLST</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGD3</accession>
    <name>D5GGD3_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Protein farnesyltransferase subunit beta</fullName>
            <shortName evidence="9">FTase-beta</shortName>
            <ecNumber evidence="2 9">2.5.1.58</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00007324001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the transfer of a farnesyl moiety from farnesyl diphosphate to a cysteine at the fourth position from the C-terminus of several proteins. The beta subunit is responsible for peptide-binding.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-cysteinyl-[protein] + (2E,6E)-farnesyl diphosphate = S-(2E,6E)-farnesyl-L-cysteinyl-[protein] + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13345"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11535"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:86019"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the protein prenyltransferase subunit beta family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.58" evidence="2 9"/>
    <dbReference type="EMBL" id="FN430264">
        <property type="protein sequence ID" value="CAZ83576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839385.1">
        <property type="nucleotide sequence ID" value="XM_002839339.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGD3"/>
    <dbReference type="FunCoup" id="D5GGD3">
        <property type="interactions" value="692"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GGD3"/>
    <dbReference type="GeneID" id="9183174"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007324001"/>
    <dbReference type="eggNOG" id="KOG0365">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028946_1_0_1"/>
    <dbReference type="InParanoid" id="D5GGD3"/>
    <dbReference type="OMA" id="HFHTGIA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005965">
        <property type="term" value="C:protein farnesyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004660">
        <property type="term" value="F:protein farnesyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097354">
        <property type="term" value="P:prenylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02893">
        <property type="entry name" value="FTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026872">
        <property type="entry name" value="FTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045089">
        <property type="entry name" value="PGGT1B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001330">
        <property type="entry name" value="Prenyltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774:SF6">
        <property type="entry name" value="PROTEIN FARNESYLTRANSFERASE SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00432">
        <property type="entry name" value="Prenyltrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 9" id="KW-0637">Prenyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Prenyltransferase alpha-alpha toroid" evidence="11">
        <location>
            <begin position="139"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="405"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="19"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="61"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="77"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="410"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ83576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="551" mass="60489" checksum="52926790A43AF6D8" modified="2010-06-15" version="1">MPQRLRVRSKRPKVFFTHNKMSATSSPSSPKAPSNSTTRTPSTPSEVFDDILSSAQPPPTYSQQTWNPSNPYFPHYHNQNMSTTATTPPESLIPPTPALFTTRPILQDTLKTLSSKEQLSTAEICMKYLSNPSDPFPILSRREHIAFLKSGVLGVQNGKLREEMVALDASRPWIIYWCLQGLVSLGVDVGKYRERVISSLQPLQNESGGFGGGNGQVSHVTASYAAVLALAVVGRGEDGDEEYGEALGIVDRRAMFRWLHEIKDWESGGFRVCVGGEEDVRGVYCALVILALLGLPTSGNLTQGTKEYLGRCQTYEGGFGATPNGNEAHGGYAFCTLAGLCILGEPAVVLRKYLDMDRAISWLSARQYAPEGGFSGRTNKLVDGCYSTWVGGCWALIEAGINGPQDENEEKLGEKEVGGLRDKPRKGPDYYHSNYILLGLASTQNYFYYTSSPIPLSSPPPSPNRGSGDPPLPLTAPFLWRYSPRIPSNTPNVAEAIVKDMPAWPDDVTEEDLLPGQEVESDRLKVHHPIFNIPFERAGEIEAWWKGKVGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGR6</accession>
    <name>D5GGR6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="13">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00007484001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430275">
        <property type="protein sequence ID" value="CAZ83688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839497.1">
        <property type="nucleotide sequence ID" value="XM_002839451.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGR6"/>
    <dbReference type="FunCoup" id="D5GGR6">
        <property type="interactions" value="90"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GGR6"/>
    <dbReference type="GeneID" id="9185610"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007484001"/>
    <dbReference type="eggNOG" id="ENOG502QTKT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027285_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGR6"/>
    <dbReference type="OMA" id="KRTMITE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000111">
        <property type="entry name" value="Soluble cell wall protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050732">
        <property type="entry name" value="Beta-glucan_modifiers"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000490">
        <property type="entry name" value="Glyco_hydro_17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16631:SF14">
        <property type="entry name" value="FAMILY 17 GLUCOSIDASE SCW10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16631">
        <property type="entry name" value="GLUCAN 1,3-BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00332">
        <property type="entry name" value="Glyco_hydro_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0134">Cell wall</keyword>
    <keyword evidence="9" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072132" evidence="12">
        <location>
            <begin position="23"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="23"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="40"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ83688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="305" mass="32508" checksum="62AF997B7E351B2E" modified="2010-06-15" version="1">MIFVRLSNIALALLAVLQLSSAQPHGGRHGRRFATPDEVGTTPTTSSTKSKGGIVYSPYNADGTCKDEATFLSDTEKLRDFSYLRLYGIDCNQVPNGIAAAKKYDFQLFLGIHNVPDCENEIGKLITMVANNWALVHTVSVGNEVVNSGQMSAESVVAKVKIARSLLGAAGYTGSVVTVDTFVAIMANPVLCEASDYVAANCHPFFDGKVDASAAGDFLKTQSENVKKACGGKKVLITETGWPHEGSPNGAAVPSVDNQGVALSSIKNAMGLDVCYFTAFDDNWKTDTPATFNAEKHWGIQNLGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>D5GH31</accession>
    <name>D5GH31_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00007681001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430297">
        <property type="protein sequence ID" value="CAZ83824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839633.1">
        <property type="nucleotide sequence ID" value="XM_002839587.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GH31"/>
    <dbReference type="STRING" id="656061.D5GH31"/>
    <dbReference type="GeneID" id="9185078"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007681001"/>
    <dbReference type="HOGENOM" id="CLU_1180945_0_0_1"/>
    <dbReference type="InParanoid" id="D5GH31"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055264">
        <property type="entry name" value="BOD1/SHG1_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05205">
        <property type="entry name" value="COMPASS-Shg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BOD1/SHG1" evidence="2">
        <location>
            <begin position="50"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ83824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="235" mass="26561" checksum="E04C6CF07ECE410B" modified="2010-06-15" version="1">MSDSYRPSNSPLTNGHHHRHRPTSTLPPPHPSLPPAPILSEEAKSTIDLLKTTYKKRGHFDSLRKDIYQSFTDSDTKLHFLTALEKIASAEIERDPSLLARDKKKAAELIEATVLRGDTYSNVEKAVEELLAARRAEIEGMLRGIMEEQGLLPRDEEEERSEKEGKDRDGDADMALYEYGIIPALEESERECWIFPKPNLQAQLQQHPHSNLNDANLPYEDDDSSQSEGTSCEIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHE5</accession>
    <name>D5GHE5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007830001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430318">
        <property type="protein sequence ID" value="CAZ83938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839747.1">
        <property type="nucleotide sequence ID" value="XM_002839701.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHE5"/>
    <dbReference type="STRING" id="656061.D5GHE5"/>
    <dbReference type="GeneID" id="9183592"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007830001"/>
    <dbReference type="eggNOG" id="KOG1840">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_125_15_1"/>
    <dbReference type="InParanoid" id="D5GHE5"/>
    <dbReference type="OMA" id="GMKHQST"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053137">
        <property type="entry name" value="NLR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46082:SF6">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46082">
        <property type="entry name" value="ATP/GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13374">
        <property type="entry name" value="TPR_10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="418" mass="46883" checksum="83BCA288E25D9669" modified="2010-06-15" version="1">MHRRAFAGLEKTAGVAHRGTSHSANNPGAVLHDQKKNSGVEVTHRHVAADGKRALGPDRPDVLLGVFRLAITLLAQRKYDEAEIMYRHGLRAMEKAVGPNNPDALDSVNMLGETLLDLERYDEAEAICRRTYAGREKALGADHQATLTSADNLGRTLYHQRKYEEAEIMFQSAQAGRRKVLGLYHPDTLLSVLRLGVTLLDRKKYKEAEIMYRHALGAMEKALGPDHPEVLDSMNTLGETLLDLKEYGEAEAMCRRALVGRERTLGPDHRDTLLSANNLGLALRNQNKHHEAEISYRRALVGAEKTLGLGHQDTLMCIYNLAGALHKQKIYDEAEMMHRRALVGREKLLGLGHRHTLRSLIKLGTVLYEKRKYDEAEVMYRRAVTEAERALGPGHPVTLKNFNHLSMFLYARATVAWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHH9</accession>
    <name>D5GHH9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00007891001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430318">
        <property type="protein sequence ID" value="CAZ83972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839781.1">
        <property type="nucleotide sequence ID" value="XM_002839735.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHH9"/>
    <dbReference type="GeneID" id="9183617"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007891001"/>
    <dbReference type="HOGENOM" id="CLU_3392569_0_0_1"/>
    <dbReference type="InParanoid" id="D5GHH9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ83972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="32" mass="3403" checksum="F84C54F71323DE60" modified="2010-06-15" version="1">MPWCSAGKFEEWGMLQVNCGGCAGPRRCGAIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHS2</accession>
    <name>D5GHS2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">U3 small nucleolar ribonucleoprotein protein MPP10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00008013001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in nucleolar processing of pre-18S ribosomal RNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
            <location evidence="1 7">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the MPP10 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430320">
        <property type="protein sequence ID" value="CAZ84033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839842.1">
        <property type="nucleotide sequence ID" value="XM_002839796.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHS2"/>
    <dbReference type="FunCoup" id="D5GHS2">
        <property type="interactions" value="901"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GHS2"/>
    <dbReference type="GeneID" id="9183687"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008013001"/>
    <dbReference type="eggNOG" id="KOG2600">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011271_1_0_1"/>
    <dbReference type="InParanoid" id="D5GHS2"/>
    <dbReference type="OMA" id="HFAEDFG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034457">
        <property type="term" value="C:Mpp10 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005732">
        <property type="term" value="C:sno(s)RNA-containing ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012173">
        <property type="entry name" value="Mpp10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17039">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RIBONUCLEOPROTEIN PROTEIN MPP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17039:SF0">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RIBONUCLEOPROTEIN PROTEIN MPP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04006">
        <property type="entry name" value="Mpp10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017300">
        <property type="entry name" value="snoRNP_Mpp10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 7" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3 7" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="113"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="326"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="568"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="128"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="213"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="295"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="326"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="592"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="611"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="638"/>
            <end position="654"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR017300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ84033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="663" mass="73536" checksum="9515CC7763938685" modified="2010-06-15" version="1">MNGSSSSLLIKALSNSQHEFLQPKSSLHSAALVLAKKYLDPLAASVAGGKKSEVLEKIYLDGFDVDQVWEQARLVVDAVESGVGEGKGVSWGVNGDAKGGELANGKKRKAVAFEEDIDSASGTSSQSDSEEDEEVDDDEDLGEEEYEEPMDEDGGIALPDDDDDEEGIEEDSEEGGEEFVQDVHGLNDGFFSIDDFNRQTRLLEMQDERGELGSDEEEIDYDADPDQLADNDEVEDEGGIVTVGNDDYDEEDGDLHDSYDNSNGIMYQDFFAPPAKKGSKKRLSKVSKPWNQRTKRLEEELEREGSNIESEMARVRRDLFDDDVSEEGSDFDMDDDLANPISHRSTHEKRQATIIEQIRQLEAANVAKKQWTLAGEARSTTRPLNSLLEEDLDFERAGKPVPVITQEVTEDLEEMIKRRIIASDFDEVIRRRPDDISSRFRRGRIELDDSKPQQGLAEIYEREHLQKIDPENNPDIKDEKLAEAHREIGELFSDVSRKLDALSSWHFTPKPPKPAISIVVDAPAISMEEAQPSAVDGGALAANISMLAPQEVYAPQKGRAVATIGEGGGREIVGKSGAPVSTREMTREDKQRRRRREKEKIKKRNLGEGNKGGEVKEGSRKDVRNSLRKGRVKVIGEGGEKRDVDGNLVKDKGGGTKSSFLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHT2</accession>
    <name>D5GHT2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00008023001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIGU family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430320">
        <property type="protein sequence ID" value="CAZ84043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839852.1">
        <property type="nucleotide sequence ID" value="XM_002839806.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHT2"/>
    <dbReference type="FunCoup" id="D5GHT2">
        <property type="interactions" value="648"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GHT2"/>
    <dbReference type="GeneID" id="9183697"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008023001"/>
    <dbReference type="eggNOG" id="KOG2552">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030193_0_0_1"/>
    <dbReference type="InParanoid" id="D5GHT2"/>
    <dbReference type="OMA" id="ALWHLWI"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042765">
        <property type="term" value="C:GPI-anchor transamidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016255">
        <property type="term" value="P:attachment of GPI anchor to protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009600">
        <property type="entry name" value="PIG-U"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13121">
        <property type="entry name" value="GPI TRANSAMIDASE COMPONENT PIG-U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13121:SF0">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL GLYCAN ANCHOR BIOSYNTHESIS CLASS U PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06728">
        <property type="entry name" value="PIG-U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="127"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="169"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="209"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="246"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="339"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="373"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ84043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="417" mass="46163" checksum="0413BCFB02F1D5B4" modified="2010-06-15" version="1">MDRRSFTVIFAAVVLRLVLFTAFPSLPDLLTNRVELSTPVTSFKRLQEGLFLYTHGLSPYDGGVFHQAPLLLAFFTTLPSNPIVVYLVYILSDVLSAHSLIQIATSGVSVSTPYHTSPRKSSTWPPWAVAAAFLFNPFSIATCIARPTIVFTNTVILTAIAKGVQGQGVAAVLALAMASYLSLYPVLLLPPMVLLAYDSLPVVKKGRYLGSFAVMLTSVFTVAAAGLGALSWGITGGSWEFLASTYGVHLLLPDLTPNVGLWWYFFIEMFDSFREFFLCVFQLHLAVYLAGLCLRLRKQPLFVITTLLGISSIFKSYPSIGDTALYLSLLSLYRHIFPLMRYTFFVTSTLLYATLLGPAFYHLWIYAGSGNANFFYAITLVWSLAVTVLVSDAIFAVLRDEWETERPEMKGKEIVQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHZ6</accession>
    <name>D5GHZ6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00008207001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIMIBI class G3E GTPase family. UreG subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430322">
        <property type="protein sequence ID" value="CAZ84139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839948.1">
        <property type="nucleotide sequence ID" value="XM_002839902.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHZ6"/>
    <dbReference type="STRING" id="656061.D5GHZ6"/>
    <dbReference type="GeneID" id="9182552"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008207001"/>
    <dbReference type="eggNOG" id="ENOG502QR6E">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072144_0_0_1"/>
    <dbReference type="InParanoid" id="D5GHZ6"/>
    <dbReference type="OMA" id="KMRGDKP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043419">
        <property type="term" value="P:urea catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05540">
        <property type="entry name" value="UreG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000208">
        <property type="entry name" value="Urease accessory protein UreG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01389">
        <property type="entry name" value="UreG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003495">
        <property type="entry name" value="CobW/HypB/UreG_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004400">
        <property type="entry name" value="UreG"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00101">
        <property type="entry name" value="ureG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31715">
        <property type="entry name" value="UREASE ACCESSORY PROTEIN G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31715:SF0">
        <property type="entry name" value="UREASE ACCESSORY PROTEIN G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02492">
        <property type="entry name" value="cobW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0996">Nickel insertion</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobW/HypB/UreG nucleotide-binding" evidence="7">
        <location>
            <begin position="49"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ84139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="251" mass="27429" checksum="6C52008ABFF88ECB" modified="2010-06-15" version="1">MTHTHDLSGGNINSHDHGHEHEILDGPGSYLARELPIVEGRNWEDRAFTIGIGGPVGSGKTALMLQLCKNLREHYSIAAVTNDIFTKEDQEFLVKNEALEPKRIRAIETGGCPHAAVREDISQNLHALEDLHREFDTQLLLIESGGDNLAANYSRELADYIIYVIDVSGGDKIPRKGGPGITQSDLLIVNKTDLAELIGADLDVMDRDSRRMREGGPTIFAQVKNGVGVEDIVSMILSAWRASGASGPWVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GI00</accession>
    <name>D5GI00_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00008212001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic/archaeal PrmC-related family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430322">
        <property type="protein sequence ID" value="CAZ84143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839952.1">
        <property type="nucleotide sequence ID" value="XM_002839906.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GI00"/>
    <dbReference type="FunCoup" id="D5GI00">
        <property type="interactions" value="329"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GI00"/>
    <dbReference type="GeneID" id="9184779"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008212001"/>
    <dbReference type="eggNOG" id="KOG3191">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018398_6_0_1"/>
    <dbReference type="InParanoid" id="D5GI00"/>
    <dbReference type="OMA" id="DVNRNAC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035657">
        <property type="term" value="C:eRF1 methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052190">
        <property type="entry name" value="Euk-Arch_PrmC-MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45875">
        <property type="entry name" value="METHYLTRANSFERASE N6AMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45875:SF1">
        <property type="entry name" value="METHYLTRANSFERASE N6AMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ84143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="228" mass="24522" checksum="A98C30C486E45F06" modified="2010-06-15" version="1">MLPTPTTSHLSFDHIYEPSEDTFLLLDTLALQSPFLRSRFPQTSLPLALELGSGSGVITSFLTTHSATLFGHTSITTLSTDISPHAPDATKQTVSANITEESGCFLGAIRTDLATGLRPGVVDMLVFNPPYVPTEVVEDGNGEEGWIGVACGGGVDGMEVTIRVLKGLKECLSESGVAYILLCERNKPEAVAEQLRNGEYGVKWIVEKVGTSGRKGGWEVLGIWRVMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>D5GI50</accession>
    <name>D5GI50_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00008277001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430323">
        <property type="protein sequence ID" value="CAZ84193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840002.1">
        <property type="nucleotide sequence ID" value="XM_002839956.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GI50"/>
    <dbReference type="STRING" id="656061.D5GI50"/>
    <dbReference type="GeneID" id="9181911"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008277001"/>
    <dbReference type="eggNOG" id="KOG0101">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1230691_0_0_1"/>
    <dbReference type="InParanoid" id="D5GI50"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ84193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="225" mass="24904" checksum="82974C01EF003756" modified="2010-06-15" version="1">MLLGRLRKSRALTRMFVADLGCTRCDTAVVTSRGGMWCTVLYTIPAMTHNYALSGVKLDEVLVDHFSKEFFQKDKVDPENERSLATLILEPKGTNRLLSLGTSATISIVPLVDGNDFHSTINRLRYELSPKVFNQIAFLVEEIVKKAALDMLDITRFVIPSGGTSHPPKIASLIAKFSVRKCPSNTHSGTQYITTLGPWSSNPTFPNCQIQQGKTSSNLPTLQYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIC7</accession>
    <name>D5GIC7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00008427001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA mismatch repair MutL/HexB family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430326">
        <property type="protein sequence ID" value="CAZ84270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840079.1">
        <property type="nucleotide sequence ID" value="XM_002840033.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIC7"/>
    <dbReference type="FunCoup" id="D5GIC7">
        <property type="interactions" value="60"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GIC7"/>
    <dbReference type="GeneID" id="9184628"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008427001"/>
    <dbReference type="eggNOG" id="KOG1978">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_449183_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIC7"/>
    <dbReference type="OMA" id="WINTEHE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032389">
        <property type="term" value="C:MutLalpha complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061982">
        <property type="term" value="P:meiosis I cell cycle process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000017">
        <property type="entry name" value="PMS1 homolog 1, mismatch repair system component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014762">
        <property type="entry name" value="DNA_mismatch_repair_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013507">
        <property type="entry name" value="DNA_mismatch_S5_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002099">
        <property type="entry name" value="MutL/Mlh/PMS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038973">
        <property type="entry name" value="MutL/Mlh/Pms-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00585">
        <property type="entry name" value="mutl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MLH, PMS, MUTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073:SF41">
        <property type="entry name" value="MISMATCH REPAIR PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_8G05820)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01119">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13589">
        <property type="entry name" value="HATPase_c_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01340">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00058">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair protein S5" evidence="4">
        <location>
            <begin position="222"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="390"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="429"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="449"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ84270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="608" mass="65565" checksum="C126E0B20CADC2D6" modified="2010-06-15" version="1">MGIQALPDTAVRALGSGQVLTDPVSLIKELLDNSLDALATAISIEVSQNTLDVLQVKDNGHGIPTEDREFVAMRHCTSKITDFEEIQDCRSLGFRGEALASAAELAGGLVITTMVEGEKVAMACTCDRVGKVTARKPVGAMKGTTVRVTDFFKFLPVRRENAIKGAQKSLSKVRTLLQRYYLSHPACRFSLRVLAPPQKSKAAGGQSEDIVYAPSKSVSEAVMKAVGKEIVATCQWINTEHEEVTDGSGEQKIGIEAFMPKPGSDPAIICRKPQCTNFVFVDSRPVSCARGTLKQILSLYKSYLKSALPDSAISDPFLYLNLRCPPGAYDPNIEPAKDDVMFFHADRVIGAVEVIFKGLYGELKTKSSPGGSGGKGKSVEMMTGGFELLLARKRDPKPGTTKPPEAPEIDEDEEEMAAQQVNMMMELDEDPPAAGLTPANPGKAPPVQTREESPHRSHNRDGSVRPPMFSITDAPSPGSVRPGELNQEPTPPAAPLAQMVQTPRRRPTNWGFNMSSGYDEDESDLVLDEVERQVRSDLAEEQADEAAARDIAISNPWTAAKMNSPASGSNQPFSPAIVPSIWSSTVSSLLCDSCIVLATDDHNAPNSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIP2</accession>
    <name>D5GIP2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00008594001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430328">
        <property type="protein sequence ID" value="CAZ84385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840194.1">
        <property type="nucleotide sequence ID" value="XM_002840148.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIP2"/>
    <dbReference type="STRING" id="656061.D5GIP2"/>
    <dbReference type="GeneID" id="9183319"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008594001"/>
    <dbReference type="eggNOG" id="ENOG502SDFJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1225549_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIP2"/>
    <dbReference type="OMA" id="ECTIART"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007741">
        <property type="entry name" value="Ribosomal_mL43/mS25/NADH_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040049">
        <property type="entry name" value="Ribosomal_mS25/mL61"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13274">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN S25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13274:SF2">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05047">
        <property type="entry name" value="L51_S25_CI-B8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00916">
        <property type="entry name" value="L51_S25_CI-B8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein/NADH dehydrogenase" evidence="6">
        <location>
            <begin position="101"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="206"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ84385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="226" mass="25491" checksum="4AA18BB0878FF8B8" modified="2010-06-15" version="1">MIFEPRFLAPPPAAVMKDRVVLCFRLDLLVACFGGSRGGGTEIATPRSEHRKPQTKPPSKMVSTKIRMFHLRSRLALIRAGTGAAILPPEVRKLGLTFAMKNTDGHMGPRKFWRNYLPRLKYHNPNVDMQVHRDRESGGDAMLVIEFDGGKTETIDVKHKHENDIVRAVLESTKAKPVPTSEEDAEIVAQYELYKVRMEEQLKINKRKRAERRAEKSAIGGIGRPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJ05</accession>
    <name>D5GJ05_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00008765001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840307.1">
        <property type="nucleotide sequence ID" value="XM_002840261.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJ05"/>
    <dbReference type="STRING" id="656061.D5GJ05"/>
    <dbReference type="GeneID" id="9181839"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008765001"/>
    <dbReference type="eggNOG" id="KOG0691">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045732_3_1_1"/>
    <dbReference type="InParanoid" id="D5GJ05"/>
    <dbReference type="OMA" id="HFLQIAY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000390">
        <property type="term" value="P:spliceosomal complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12429">
        <property type="entry name" value="RRM_DNAJC17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034254">
        <property type="entry name" value="DNAJC17_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052094">
        <property type="entry name" value="Pre-mRNA-splicing_ERAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44313">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44313:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="8">
        <location>
            <begin position="18"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="105"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="148"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="269"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="354"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="157"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="281"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="305"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="326"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ84498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="384" mass="43259" checksum="4C48DF79987BDA65" modified="2010-06-15" version="1">MPSTESDAASYAATSETDFYELLELPSSNTPLTPSLLRKSYRKASLKWHPDKNPSPEAAEIFYLLSIAYDVLSDPATRAVYDNARNARLARKRRNEAFDVNRKRMQEELESRERGAKKARTDGEDAEERFRRQLEKLRAEGAELRRKREGAMRAAVEEEEKGGEEEGEAMADGDGGSNGGSRFSEIDRTLRVRWKLKGNEHFDEQHLRSIFSKYGAIQDCVVPPTKSEKEKKLKSALIVFTSIVAAHAAFHDPLDSRLKDLVWASGKEPDISHNHHHYHPTPEPHTPPPPKPKQQTPKDPPKPPTLARPTTTTTKDPKLNRVPSFASFSGTPRGSPSNRSAIAQSPDYESITLMRMRAAEKAKIEARIRKEEEEEAAALEEREV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJ29</accession>
    <name>D5GJ29_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00008810001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prefoldin subunit beta family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840331.1">
        <property type="nucleotide sequence ID" value="XM_002840285.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJ29"/>
    <dbReference type="FunCoup" id="D5GJ29">
        <property type="interactions" value="727"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJ29"/>
    <dbReference type="GeneID" id="9181863"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008810001"/>
    <dbReference type="eggNOG" id="KOG4098">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113004_1_0_1"/>
    <dbReference type="InParanoid" id="D5GJ29"/>
    <dbReference type="OMA" id="CFKMIGG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23163">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.370:FF:000002">
        <property type="entry name" value="Prefoldin subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027235">
        <property type="entry name" value="PFD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002777">
        <property type="entry name" value="PFD_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13303">
        <property type="entry name" value="PREFOLDIN SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01920">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="40"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="111"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="16"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ84522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="146" mass="16331" checksum="AD2BE1C1C9AD8195" modified="2010-06-15" version="1">MSTAPSPSVAKKQQGPPFPPLCPLPLPPHTTNSQLTKTKKTDLQTQYSTYKSALQQLAQKIGDVEQEAEEHKLVLESLEPVDPGRKCFRLVNGVLVERCVGDVIPAVRTNAEGLKSVLEGLVKEYKRKQEEMEKWKVKNNVQVVNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJ53</accession>
    <name>D5GJ53_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00008842001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840355.1">
        <property type="nucleotide sequence ID" value="XM_002840309.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJ53"/>
    <dbReference type="GeneID" id="9181887"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008842001"/>
    <dbReference type="eggNOG" id="ENOG502SWNK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099493_1_0_1"/>
    <dbReference type="InParanoid" id="D5GJ53"/>
    <dbReference type="OMA" id="VPQNHFT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020999">
        <property type="entry name" value="Chitin_synth_reg_RCR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12273">
        <property type="entry name" value="RCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ84546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="177" mass="20528" checksum="50DD19F3313C6FD3" modified="2010-06-15" version="1">MAVLKRWLDLVKRDHGRDDDDWPHRSWYWNDQARAIKWGIFFGILTFFFVLMMAMYYHADRRMKNGQPPLRYHRWLVPRTRRAQFEPNLQRPEENFSFYHQQQPQENDTYGMHAVPPPAYNPNYPEPPVYSGGPPIGATKVDPAEHPAHHLGEGSSSGPAPPPPVAVPASTPQQRPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJ63</accession>
    <name>D5GJ63_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00008858001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840365.1">
        <property type="nucleotide sequence ID" value="XM_002840319.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJ63"/>
    <dbReference type="STRING" id="656061.D5GJ63"/>
    <dbReference type="GeneID" id="9185924"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008858001"/>
    <dbReference type="eggNOG" id="KOG4628">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031409_1_0_1"/>
    <dbReference type="InParanoid" id="D5GJ63"/>
    <dbReference type="OMA" id="LMKYKTW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16473">
        <property type="entry name" value="RING-H2_RNF103"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000539">
        <property type="entry name" value="Ring finger domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051826">
        <property type="entry name" value="E3_ubiquitin-ligase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22765:SF434">
        <property type="entry name" value="GB|AAD18119.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22765">
        <property type="entry name" value="RING FINGER AND PROTEASE ASSOCIATED DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="30"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="4">
        <location>
            <begin position="229"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="64"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="186"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="278"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="329"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="186"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="331"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ84556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="417" mass="45420" checksum="FCD1AA0B399C4E68" modified="2010-06-15" version="1">MSTTLPVIPSPSPTQTGNGGGNNGPTSSPLLFFVALGFGVVFTNLWIIVGVKYCFRYNARQRRRANGEEEPVDLQTMPRPHRRRREKKLMTMDEVNERFPTTKYKSWRAHRERMGLPTEGGIKTAPNSRPASLMDLEAATSADANNGSPDALAKELTLPPAVDKETSPAPAPTLFTDKLEAPEIARKHLSDISEPEVRKCEDDEDEEDEHDHVQTAVPDELLNSVGDSCAICLDTIEDDDDIRGLTCGHAFHAGCLDPWLTSRKACCPLCKADYYVPKPRPEGENVPDDQSRSHRHRRGTNGTPTPPANVYLVPRRMLFSPRRIISPYYTGSASGRSRGGSSVAAQRGSEDGQYTGGSRWMRNPLRGVSLSFGRRNNTAAPPTPSNLEAGLLDNTPAAAAPSTGVPTPNPISGAEAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJE1</accession>
    <name>D5GJE1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00008957001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GPAT/DAPAT family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430330">
        <property type="protein sequence ID" value="CAZ84634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840443.1">
        <property type="nucleotide sequence ID" value="XM_002840397.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJE1"/>
    <dbReference type="STRING" id="656061.D5GJE1"/>
    <dbReference type="GeneID" id="9182635"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008957001"/>
    <dbReference type="eggNOG" id="KOG3729">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015023_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJE1"/>
    <dbReference type="OMA" id="ENMICKM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004366">
        <property type="term" value="F:glycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006072">
        <property type="term" value="P:glycerol-3-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07993">
        <property type="entry name" value="LPLAT_DHAPAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022284">
        <property type="entry name" value="GPAT/DHAPAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045520">
        <property type="entry name" value="GPAT/DHAPAT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041728">
        <property type="entry name" value="GPAT/DHAPAT_LPLAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12563:SF17">
        <property type="entry name" value="DIHYDROXYACETONE PHOSPHATE ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12563">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19277">
        <property type="entry name" value="GPAT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="7">
        <location>
            <begin position="204"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ84634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="788" mass="89211" checksum="087FA518118AD5AE" modified="2010-06-15" version="1">MTSKEPSTSAPDIDILPPNLQIHPPLVPEQQLPSPNSSEESRNLITANFTHFRDDPFSFLREISLHYSGTGWRAYDEPIGQKIFYKGFSDEMKARVLDSRMLKAKLQELAGRRVVIEDEMGLFGEEKRRQKRLQEVEKQLNEVAEEIVEGMICKFESKRFIRGAYFICVQLLARTYHRGIHVSTLEIQNLRETAKLAAARKQSLIFLPCHRSHIDYVSLQVICYRLGLTLPVVVAGDNLNFPLVGPFLQHAGAMFIRRSFSGDEVYTAIVQAYVDALLSGGYNFECFIEGGRSRTGKLLAPKFGILSFILDSILSGRVQDAWFIPVSTQYDKVIETEAYVSELLGIPKVKENLWDFLSSSKFLNLNMGRVDVRFHEPWSLRTFVESQIDKIRAEGAIGISLTPRLINPADANVGQDTVDRADPEVRLKVLRSLGYRVLGDINRVSVVMPTSLIGTILLTLRGRGVGKSELVRRMEWLRTRIRAKGGRVAHFGIAETETVVERGLEVLGELVGRVDGLAEETFFAVDRFQLSFYRNMVIHLFISETIVAAAMYTRIKQGGGPANQRVEYQTLLDQVSFLSQLFRGEFVFPTEGLITNLSKTLTGLSADNVITVTSNSDSKIGYVELSDAERKCGRENFDFYCFLIWPFIEGSWLAGVAIMGLIPPKGMEEVWVDLREAQDMAQLMGKTLYHQGDISYFEAVNKETLKNAFQRFQEEGIITIRKSKSGRTPPLMRVSPDWIANDRLWEFVEKIAISRREGKNRRDNESVSSRVLKLAERCGRELFERGGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJH8</accession>
    <name>D5GJH8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00009023001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Allantoate permease family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430331">
        <property type="protein sequence ID" value="CAZ84671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840480.1">
        <property type="nucleotide sequence ID" value="XM_002840434.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJH8"/>
    <dbReference type="FunCoup" id="D5GJH8">
        <property type="interactions" value="113"/>
    </dbReference>
    <dbReference type="GeneID" id="9182672"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009023001"/>
    <dbReference type="eggNOG" id="KOG2533">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_0_5_1"/>
    <dbReference type="InParanoid" id="D5GJH8"/>
    <dbReference type="OMA" id="TSNRWAW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17327">
        <property type="entry name" value="MFS_FEN2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000064">
        <property type="entry name" value="MFS allantoate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF40">
        <property type="entry name" value="THIAMINE PATHWAY TRANSPORTER THI73"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="58"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="130"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="217"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="286"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="320"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="350"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="376"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="411"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="444"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="58"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ84671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="502" mass="54567" checksum="B0370F18B263B54C" modified="2010-06-15" version="1">MSEKQTGTVSDRSTSGSIPPEVGNNSNVDIALTYVRNVGAGGGHVGQKELVRKIDWRIVPIMFACYTMQFLDKVLINYAAVMGMPKDLKLKGNDLTWAATSFFVAYLVAEIPTGYLLQKMPAGKYLGANVVLWGIVTVCTAATNSFTGFVVARVFLGIFEAAIAPCLTIITGMWYTTSEQPPRFAFWYCGLGIGQIVGGLVSYGFQQIKGESLEGWRVMFIVLGLVTILIGSVTYLFLPDTPMTASFLTKEEKVVAINRIADNQTGIENRTFKWSQIKELAMDVQIYLIGLVILLASISSGVITTYSSTLIRNFGYTPPIAALLNMPSGVVSLAVTILSGVAVGKASNRWLWIAGLAIPGAIGGGLMSFLPKSNRAGLLVGIYLVNSIVSVLIIVYNWLSANIAGHTKRTVAMSLVSGSFSIGNIIGPQTFQARDAPNFYPAKIAVLATQASAALAAIVLRLYYGWQNSKREKRSEIPHSLPEDYKWLNLTDKENPNFRYVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJN3</accession>
    <name>D5GJN3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00009085001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GET4 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430332">
        <property type="protein sequence ID" value="CAZ84726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840535.1">
        <property type="nucleotide sequence ID" value="XM_002840489.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJN3"/>
    <dbReference type="FunCoup" id="D5GJN3">
        <property type="interactions" value="375"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJN3"/>
    <dbReference type="GeneID" id="9186760"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009085001"/>
    <dbReference type="eggNOG" id="KOG3024">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046061_0_1_1"/>
    <dbReference type="InParanoid" id="D5GJN3"/>
    <dbReference type="OMA" id="LMDMMGM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072380">
        <property type="term" value="C:TRC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045048">
        <property type="term" value="P:protein insertion into ER membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000060">
        <property type="entry name" value="Golgi to ER traffic protein 4 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007317">
        <property type="entry name" value="GET4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12875">
        <property type="entry name" value="GOLGI TO ER TRAFFIC PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12875:SF0">
        <property type="entry name" value="GOLGI TO ER TRAFFIC PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04190">
        <property type="entry name" value="GET4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="304"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="304"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ84726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="325" mass="36303" checksum="D4AB784CC4E19E29" modified="2010-06-15" version="1">MASKNVEKTRARLEKRIEEGAYYEAHQQLRVVSQRYIKAHNYDAAIDILCSGAEALLKAGQNSSGCDLCLMLVGVYKTGSLAPSATSRARLIQLISLIPPEEPGRKRFINEAIAWSSKHGEYPAGDPELHHFIGKLLAQEDDTYEAEKHLVVGTKESAETFADMLYEWYTEDEPHTAPIYAARAVLPYMLIGNLRDATRLLTTFTRKLIENNSSLAVQEVQTASVDCKVVPSLPLLNFLNLLLLACQTGGQDMFRNLKSHYSNSLMEVPHWNEPLEQIGEIYLGIQIRRPASIFDMMGSMFGGTPNSVPQSRQIPPEQQPLVDLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5GK34</accession>
    <name>D5GK34_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00009342001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430335">
        <property type="protein sequence ID" value="CAZ84877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840686.1">
        <property type="nucleotide sequence ID" value="XM_002840640.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GK34"/>
    <dbReference type="GeneID" id="9186914"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009342001"/>
    <dbReference type="HOGENOM" id="CLU_3242470_0_0_1"/>
    <dbReference type="InParanoid" id="D5GK34"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ84877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="43" mass="4815" checksum="F84C8733E20D7BF3" modified="2010-06-15" version="1">MALGLFRSYALSPHSLLFLHSVNQKASTFVSFLPFLPFIICIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GK64</accession>
    <name>D5GK64_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ER lumen protein-retaining receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00009395001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Endoplasmic reticulum membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the ERD2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="FN430337">
        <property type="protein sequence ID" value="CAZ84907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840716.1">
        <property type="nucleotide sequence ID" value="XM_002840670.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GK64"/>
    <dbReference type="FunCoup" id="D5GK64">
        <property type="interactions" value="342"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GK64"/>
    <dbReference type="GeneID" id="9181998"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009395001"/>
    <dbReference type="eggNOG" id="KOG3106">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057784_0_0_1"/>
    <dbReference type="InParanoid" id="D5GK64"/>
    <dbReference type="OMA" id="WKSRSCE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045015">
        <property type="term" value="F:HDEL sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006621">
        <property type="term" value="P:protein retention in ER lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000133">
        <property type="entry name" value="ER_ret_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10585">
        <property type="entry name" value="ER LUMEN PROTEIN RETAINING RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00810">
        <property type="entry name" value="ER_lumen_recept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00660">
        <property type="entry name" value="ERLUMENR"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00951">
        <property type="entry name" value="ER_LUMEN_RECEPTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00952">
        <property type="entry name" value="ER_LUMEN_RECEPTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="62"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="96"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="151"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ84907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="213" mass="24812" checksum="0E9FBB911866D7C5" modified="2010-06-15" version="1">MNLFRIAGDLSHLISIFILLHKMRISKSCIGISFKSQVLYLIVYVTRYLDLLWTFQHSVYNSLMKIIFIGSSAYTIYLMLNDFKPTHDPNIDTFKVEYLIGGSAILALLFPYKWTTAEVLWSFSIWLESVAILPQLFMLQRTGAGETITTHYIFALGAYRALYIPNWLYRYFMENYTDPIATVAGIIQTILYSDFFYIYYTTVSKGRKFELPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>D5GK85</accession>
    <name>D5GK85_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">NADH-ubiquinone oxidoreductase 78 kDa subunit, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">GSTUM_00009423001</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the complex I 75 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430337">
        <property type="protein sequence ID" value="CAZ84928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840737.1">
        <property type="nucleotide sequence ID" value="XM_002840691.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GK85"/>
    <dbReference type="STRING" id="656061.D5GK85"/>
    <dbReference type="GeneID" id="9186957"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009423001"/>
    <dbReference type="eggNOG" id="KOG2282">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000422_11_6_1"/>
    <dbReference type="InParanoid" id="D5GK85"/>
    <dbReference type="OMA" id="QAMAYGV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02773">
        <property type="entry name" value="MopB_Res-Cmplx1_Nad11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.740:FF:000016">
        <property type="entry name" value="NADH dehydrogenase (Quinone), G subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.740:FF:000001">
        <property type="entry name" value="NADH-quinone oxidoreductase subunit G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.210:FF:000002">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase 75 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.20:FF:000002">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase 75 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006963">
        <property type="entry name" value="Mopterin_OxRdtase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000283">
        <property type="entry name" value="NADH_UbQ_OxRdtase_75kDa_su_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054351">
        <property type="entry name" value="NADH_UbQ_OxRdtase_ferredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010228">
        <property type="entry name" value="NADH_UbQ_OxRdtase_Gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019574">
        <property type="entry name" value="NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015405">
        <property type="entry name" value="NDUFS1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050123">
        <property type="entry name" value="Prok_molybdopt-oxidoreductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01973">
        <property type="entry name" value="NuoG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43105:SF13">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 75 KDA SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43105">
        <property type="entry name" value="RESPIRATORY NITRATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13510">
        <property type="entry name" value="Fer2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22151">
        <property type="entry name" value="Fer4_NDSU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22117">
        <property type="entry name" value="Fer4_Nqo3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10588">
        <property type="entry name" value="NADH-G_4Fe-4S_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09326">
        <property type="entry name" value="NADH_dhqG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00929">
        <property type="entry name" value="NADH-G_4Fe-4S_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53706">
        <property type="entry name" value="Formate dehydrogenase/DMSO reductase, domains 1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51839">
        <property type="entry name" value="4FE4S_HC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51669">
        <property type="entry name" value="4FE4S_MOW_BIS_MGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00641">
        <property type="entry name" value="COMPLEX1_75K_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00642">
        <property type="entry name" value="COMPLEX1_75K_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00643">
        <property type="entry name" value="COMPLEX1_75K_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="12">
        <location>
            <begin position="35"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S His(Cys)3-ligated-type" evidence="14">
        <location>
            <begin position="113"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S Mo/W bis-MGD-type" evidence="13">
        <location>
            <begin position="252"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00070722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAZ84928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="740" mass="80310" checksum="08B733E5B740011B" modified="2010-06-15" version="1">MMLQGLLRSLKPSGRLLATSPAPRRTFSSTLRAFDEVEITIDGKKVKIEAGAALIQACEKAGVTIPRYCYHEKLMIAGNCRMCLVEVERVPKPVASCAWPVQPGMLVKTNSPVVHKAREGVMEFLLANHPLDCPICDQGGECDLQDQSMRYGADRGRFHEIGGKRAVEDKNIGPLIKTSMNRCIHCTRCIRFANDVAGAPELGSTGRGNDMQIGTYLEKNLDSELSGNVIDLCPVGALTSKPYAFRARPWELKPVESVDVLDALGSAIRVDARGMEVMRVIPRLNDDVNEEWINDKTRFACDGLRTQRLTMPLVRRDDKFIPATWEQALAEVASAFQRLQPKGDEVKAIAGHLVEAESLVALKDMINRLGSENLALDQPLGNQPPAHGVDIRSNYLFNSKICGVEEADAILLVGTNPRHEAAVLNARIRKQWLRSDLEIGLVGENFDNIFHIEHLGQDATSLKKALAGKFGQKLLVAKRPMIIVGSGVTEHTDGKAFYEIIGQFVDKNAGKIISPEWNGYNVLQRAASRAGAYDIGFVSSSPTISQTTPKIIWLLGADEITPNDIPKGAFVVYQGHHGDVGAQYADVVLPGAAYTEKSGTYVNTEGRAQLTRAATGPPGVAREDWKIIRAASEFLGVTLPYDSLAGLRTRMEGISPALTHYDVVEPASLATVGNKVQIVDANKGAEAGGAPLRRVIENFYFTDAISRSSPTMSRCSAAKMTRSGIEADPSPGDAAYAASG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKD7</accession>
    <name>D5GKD7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00009493001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430338">
        <property type="protein sequence ID" value="CAZ84980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840789.1">
        <property type="nucleotide sequence ID" value="XM_002840743.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKD7"/>
    <dbReference type="FunCoup" id="D5GKD7">
        <property type="interactions" value="327"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GKD7"/>
    <dbReference type="GeneID" id="9182000"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009493001"/>
    <dbReference type="eggNOG" id="KOG3487">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085828_0_2_1"/>
    <dbReference type="InParanoid" id="D5GKD7"/>
    <dbReference type="OMA" id="RYMNQFI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14825">
        <property type="entry name" value="TRAPPC2_sedlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006722">
        <property type="entry name" value="Sedlin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12403">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04628">
        <property type="entry name" value="Sedlin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ84980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="141" mass="16259" checksum="3A7E2625A6E1C5CA" modified="2010-06-15" version="1">MSFYFAIVGTKDNPIYEAEFGTSKQGGDGVARFREEQRSMNQFIVHSSLDIVEEVQWATPTMYMKCVDKFNNLQVSCFLTAGNIKFLLLHDQKADDAIRQFFTDVYDLYTKTLMNPFYEVDMRITSGVFDGKVKQAAKKYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5GL18</accession>
    <name>D5GL18_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00009915001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS8 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430344">
        <property type="protein sequence ID" value="CAZ85211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841020.1">
        <property type="nucleotide sequence ID" value="XM_002840974.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GL18"/>
    <dbReference type="FunCoup" id="D5GL18">
        <property type="interactions" value="1032"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GL18"/>
    <dbReference type="GeneID" id="9185805"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009915001"/>
    <dbReference type="eggNOG" id="KOG3283">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080597_1_1_1"/>
    <dbReference type="InParanoid" id="D5GL18"/>
    <dbReference type="OMA" id="QRPHYRK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11380">
        <property type="entry name" value="Ribosomal_S8e_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.168.20">
        <property type="entry name" value="Ribosomal protein S8e, subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001047">
        <property type="entry name" value="Ribosomal_eS8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042563">
        <property type="entry name" value="Ribosomal_protein_eS8_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022309">
        <property type="entry name" value="Ribosomal_Se8/biogenesis_NSA2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00307">
        <property type="entry name" value="eS8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10394">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01201">
        <property type="entry name" value="Ribosomal_S8e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ85211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="201" mass="22190" checksum="18A57EDF19C8FA39" modified="2010-06-15" version="1">MGISRDSRHKRSATGAKRATYRKKRAFELGRQSASTRIGTKRIHLVRTRGGNRKFRAIRLEAGNFSWGSEGISKKTRVIGVVFHPSNNELVRTNTLTKSAIVQIDAAPFRQWQLPLNLQIKRGAAAKAEAAETAPTTKSKSVQRKLAARAPTAKVENALETQFAAGRLYAAISSRPGQSGRADGYILEGEELAFYQKQLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>D5GL56</accession>
    <name>D5GL56_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ATPase synthesis protein 25</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00010013001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Mitochondrial mRNA stabilization factor.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Probable mitochondrial mRNA stabilization factor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Mitochondrion inner membrane</location>
            <topology evidence="2 8">Peripheral membrane protein</topology>
            <orientation evidence="2 8">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the ATP25 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430346">
        <property type="protein sequence ID" value="CAZ85249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841058.1">
        <property type="nucleotide sequence ID" value="XM_002841012.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GL56"/>
    <dbReference type="STRING" id="656061.D5GL56"/>
    <dbReference type="GeneID" id="9182043"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010013001"/>
    <dbReference type="eggNOG" id="ENOG502RGZN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016140_0_0_1"/>
    <dbReference type="InParanoid" id="D5GL56"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140053">
        <property type="term" value="P:mitochondrial gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048255">
        <property type="term" value="P:mRNA stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040152">
        <property type="entry name" value="Atp25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28087">
        <property type="entry name" value="ATPASE SYNTHESIS PROTEIN 25, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28087:SF1">
        <property type="entry name" value="ATPASE SYNTHESIS PROTEIN 25, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02410">
        <property type="entry name" value="RsfS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ85249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="683" mass="76450" checksum="FFF0F1A235EFCCA1" modified="2010-06-15" version="1">MFGGGRAFRVSVETSGPPVSTTLAPPVPPETCDSKEGSSPWYLRVKTPILRFHPLAERQALPALPEDPPPALQAVLDQLSVEEGIDYLKVLDLRALDPAPALGENIVMVIGTARSGRQLHMCSERMCKWLRKEFNMRPFADGLVGRGELKLQQKRARRRGKLTTAESGIEEAAGWVCITTGGQGLVIQLFTQDKREEIDLESLWGKELERDAKKKEAEKRIVEGKEQGTFGAFGRGLSTGFPIPGTPRLQARSFHSPSQKPSQVVTGFSAGNLVRFAEEGISVDTHVKKGHYWRLVTRVSEAFDPDADLKNAAYLMEAHINHLRKKFRGNNYSGKLTKGCKLLGQGIEDRTSTEFLQSYFRYLPKHPAPTHLRLHLEFLIETNRISPEKYPLSKVTTFLKAFPAPIPAELYYLCMRAIADSPEHRSYKLPASEAATLRLSLIQDLAEHFDPTSTLSRNIPEFQFCRFRALAPDNAIALILLAAKTVCSTKRAEAGKEADGAIKKFNIRVGTYALDPRLVRVERESIEQMCHSYAIRDYYEQAMAAYVAADHWTALWNRWKSLKYMSIRRDQAFYELLIGLVVLGAKQKEGVYAARHIWNDMGRETPKVLMTAGIAKGLLAVIRLAERDSSASGEFAELKARCDGYLERWHKWRVENGLVEEEDAAATIAKEGKSREENGGVIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLW6</accession>
    <name>D5GLW6_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA primase large subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00010447001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="9">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the eukaryotic-type primase large subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430351">
        <property type="protein sequence ID" value="CAZ85533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841342.1">
        <property type="nucleotide sequence ID" value="XM_002841296.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLW6"/>
    <dbReference type="FunCoup" id="D5GLW6">
        <property type="interactions" value="882"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GLW6"/>
    <dbReference type="GeneID" id="9187313"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010447001"/>
    <dbReference type="eggNOG" id="KOG2267">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026253_1_0_1"/>
    <dbReference type="InParanoid" id="D5GLW6"/>
    <dbReference type="OMA" id="RINYKPW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005658">
        <property type="term" value="C:alpha DNA polymerase:primase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07322">
        <property type="entry name" value="PriL_PriS_Eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058560">
        <property type="entry name" value="DNA_primase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016558">
        <property type="entry name" value="DNA_primase_lsu_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007238">
        <property type="entry name" value="DNA_primase_lsu_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10537">
        <property type="entry name" value="DNA PRIMASE LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10537:SF3">
        <property type="entry name" value="DNA PRIMASE LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04104">
        <property type="entry name" value="DNA_primase_lrg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26466">
        <property type="entry name" value="DNA_primase_lrg_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009449">
        <property type="entry name" value="DNA_primase_large_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9 10" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4 9" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0408">Iron</keyword>
    <keyword evidence="7 9 10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5 9 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 9" id="KW-0639">Primosome</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA primase large subunit C-terminal" evidence="11">
        <location>
            <begin position="330"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="335"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="413"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="430"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="471"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR009449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR009449-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ85533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="540" mass="61841" checksum="A889BF06BF9082DA" modified="2010-06-15" version="1">MYRQEPAKRVRAIDSKKHWAGVDFKQLFYPHRLNFYNTPPTTEITLDEFEQWAIDRLRILGEIESCLYRNMTAAELQDVIIKKLQKDLLPLGANSAAGGGSKFQDSKIAKQQDERRKDHYSHYTLRLAFARSEDLRKRFLATETILFKLRYNSDDTQERQAFIESLGFDWEFLSVEEKVAMREDLLAGTGTKSNEADAVFEAGFFRVDWEKVYDLVDQRKVLLRGGKAYVPESQQSSLVIAEFTARLEKALEACLLPPQVFSSRRRNRSLTISTQLTARALPRLDEDDRLIPILNHLSRGFIAPEYNTSMTATTLNGASLTAAVIDDLVQYFPMCMRQLHTTLRREKHLLHNGRLQYNLFLKGIGLSVDEALIFWRKSFSRIDDDEFQKKYRYNVRHGYGLEGTRRNYKPMSCQQILTDRRPAANQVHGCPYREVAIDNLVVGLGTMGVSDRDVIRHVREDIEAKKYHVACSRVFEHTHAKELKREKEAGGWSGGLTENIIHPNEYFTRSFGLKNPGSVEATNAIAPPASGGSGQSMEMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMC4</accession>
    <name>D5GMC4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00010649001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bystin family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430354">
        <property type="protein sequence ID" value="CAZ85667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841476.1">
        <property type="nucleotide sequence ID" value="XM_002841430.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMC4"/>
    <dbReference type="FunCoup" id="D5GMC4">
        <property type="interactions" value="801"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GMC4"/>
    <dbReference type="GeneID" id="9183237"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010649001"/>
    <dbReference type="eggNOG" id="KOG3871">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029727_0_1_1"/>
    <dbReference type="InParanoid" id="D5GMC4"/>
    <dbReference type="OMA" id="TKLPVIW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007955">
        <property type="entry name" value="Bystin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12821">
        <property type="entry name" value="BYSTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12821:SF0">
        <property type="entry name" value="BYSTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05291">
        <property type="entry name" value="Bystin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="58"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="92"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ85667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="433" mass="49192" checksum="DB52982D03807D94" modified="2010-06-15" version="1">MPKEVAPRQGRRHNPLHDDLVENESGNLRRIARSKRKEKSESFENYVDSGSSKRILKIARDQQDELHEEEARKNAAQGEFFGSAAQMRLQDQEEEESDEEEYEEADFGEDDIVEEVEVDEGDIELFNQFMPSGQGAAQRISLADKILEKIAEHEAKLAGHPIGDEETSTLPPKIIEVYTKVGLLLSRYKSGKLPKAFKIIPSLNNWEEILSITRPDQWSPHACYEATRLFASTKANQCQKFLNVVLLDRVRDDIQENKKLNVHLYNAIKKSLYKPAAFFKGFLFPLALSGTCTLKEAQIVGSVLTRISVPVLHSAAALLRLCEMDYTGPTSVFIKVLIDKKYALPYKVIDALVFHFMRFKSVPDALPLLWHQSFLAFAQRYKNDITEDQRDVLLDVVLVKGHPLVSPEIRRELLEGRGRGVEIGEADDDLMLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMH9</accession>
    <name>D5GMH9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cytochrome b-c1 complex subunit Rieske, mitochondrial</fullName>
            <ecNumber evidence="11">7.1.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00010736001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a quinol + 2 Fe(III)-[cytochrome c](out) = a quinone + 2 Fe(II)-[cytochrome c](out) + 2 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:11484"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="7.1.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
        <text evidence="11">Binds 1 [2Fe-2S] cluster per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="11">The Rieske protein is a high potential 2Fe-2S protein.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Rieske iron-sulfur protein family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.8" evidence="11"/>
    <dbReference type="EMBL" id="FN430356">
        <property type="protein sequence ID" value="CAZ85722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841531.1">
        <property type="nucleotide sequence ID" value="XM_002841485.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMH9"/>
    <dbReference type="STRING" id="656061.D5GMH9"/>
    <dbReference type="GeneID" id="9182148"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010736001"/>
    <dbReference type="eggNOG" id="KOG1671">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_202262_0_0_1"/>
    <dbReference type="InParanoid" id="D5GMH9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008121">
        <property type="term" value="F:quinol-cytochrome-c reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd03470">
        <property type="entry name" value="Rieske_cytochrome_bc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="entry name" value="Rieske [2Fe-2S] iron-sulphur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014349">
        <property type="entry name" value="Rieske_Fe-S_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005805">
        <property type="entry name" value="Rieske_Fe-S_prot_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006317">
        <property type="entry name" value="Ubiquinol_cyt_c_Rdtase_Fe-S-su"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01416">
        <property type="entry name" value="Rieske_proteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10134">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT RIESKE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00162">
        <property type="entry name" value="RIESKE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="ISP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Rieske" evidence="13">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ85722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="62" mass="6702" checksum="BD6A3DEEBE6665F3" modified="2010-06-15" version="1">MLGICTHLGCVPIGEAGDYGGWFCPCHGSHYDISGRVRRGPAPLNLEIPEYDFPEEGKLVIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMM6</accession>
    <name>D5GMM6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00010814001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDC73 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430357">
        <property type="protein sequence ID" value="CAZ85769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841578.1">
        <property type="nucleotide sequence ID" value="XM_002841532.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMM6"/>
    <dbReference type="FunCoup" id="D5GMM6">
        <property type="interactions" value="190"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GMM6"/>
    <dbReference type="GeneID" id="9185105"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010814001"/>
    <dbReference type="eggNOG" id="KOG3786">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025849_2_0_1"/>
    <dbReference type="InParanoid" id="D5GMM6"/>
    <dbReference type="OMA" id="FRPDYWN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016593">
        <property type="term" value="C:Cdc73/Paf1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11990">
        <property type="entry name" value="RNA polymerase II accessory factor, Cdc73 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007852">
        <property type="entry name" value="Cdc73/Parafibromin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031336">
        <property type="entry name" value="CDC73_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038103">
        <property type="entry name" value="CDC73_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12466">
        <property type="entry name" value="CDC73 DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12466:SF8">
        <property type="entry name" value="PARAFIBROMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05179">
        <property type="entry name" value="CDC73_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="Cell division control protein 73 C-terminal" evidence="6">
        <location>
            <begin position="254"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="225"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="240"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ85769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="415" mass="45703" checksum="7EB11CA5DCE5EE71" modified="2010-06-15" version="1">MVEFDTLLRVPVSRPDTAMSSSSTTADTDPLSLLRLSISQSSPPSLSHTPNPPPQGVSLASASYLIFHSAAFPLWTKTRFHKSSGESVDLRSIYFAWLHREDTIPDYITKAQNLGDGVVTNLAFLERLELVTWLEGGQEESDFIKPLASAVASASASADAHATSAVSGGGVVGAAAMGAQVTDPRLLEIYSHERVLSNRNTFLRGVKPTDFSHVRKQAEDFITRLRNQKPSGNNPPPSTPATSTPSKSPSKSRSRDPIILLSPSASSLLTMANIKAFLENGSFIPSSQLPGPHETIHQISRLIPAIHPTAPVRFLVVDSIEKFKPDYWDRVVGVFTTGQAWQFRDYKWNNPVELFRQVRGFYVGWDGEAVPDQVRGWGAGVKCLSIERNRRFRDREVCESIWDGIETGMKVKFGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMS7</accession>
    <name>D5GMS7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00010913001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430359">
        <property type="protein sequence ID" value="CAZ85820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841629.1">
        <property type="nucleotide sequence ID" value="XM_002841583.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMS7"/>
    <dbReference type="STRING" id="656061.D5GMS7"/>
    <dbReference type="GeneID" id="9185156"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010913001"/>
    <dbReference type="eggNOG" id="KOG0725">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_0_1"/>
    <dbReference type="InParanoid" id="D5GMS7"/>
    <dbReference type="OMA" id="SIAQTCA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43180">
        <property type="entry name" value="3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE (AFU_ORTHOLOGUE AFUA_6G11210)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43180:SF66">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE_REDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ85820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="310" mass="31927" checksum="E7FD5CF9231A1B1B" modified="2010-06-15" version="1">MSSSAQSRLLQVAGHVGGGGGEGGLPPVPMVAGDSVGPRLTGKVAIVTGCNSAIGIGRATALQYARNNLKALFICDFSNTNLPALESELRALAPNTKIHARTFDAADEASVAAVVQEALDKYARLDIFFANAGVLGGMKLVGDITAEEFMETLRVNTLSVFLAVKYAGKAMQVVGKEKKVPGGSIIATASTAGVRSGAGTTDYSASKAAVINVMQTSAFQLAGTNVRCNAICPGLTETGMTSGVWDAARKRGTEGKIGQINPLKRGAVSDEIARVALFLGSDESSYVNAQFWLVDGGLSGSHPTAPGKFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5GN49</accession>
    <name>D5GN49_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00011090001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430362">
        <property type="protein sequence ID" value="CAZ85942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841751.1">
        <property type="nucleotide sequence ID" value="XM_002841705.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GN49"/>
    <dbReference type="FunCoup" id="D5GN49">
        <property type="interactions" value="534"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GN49"/>
    <dbReference type="GeneID" id="9185868"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011090001"/>
    <dbReference type="eggNOG" id="KOG2178">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008831_7_2_1"/>
    <dbReference type="InParanoid" id="D5GN49"/>
    <dbReference type="OMA" id="ATAWCAF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019674">
        <property type="term" value="P:NAD+ metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006741">
        <property type="term" value="P:NADP+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10330:FF:000025">
        <property type="entry name" value="NAD+ kinase Utr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.30">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017437">
        <property type="entry name" value="ATP-NAD_kinase_PpnK-typ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002504">
        <property type="entry name" value="NADK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275">
        <property type="entry name" value="NAD KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275:SF0">
        <property type="entry name" value="NAD KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01513">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20143">
        <property type="entry name" value="NAD_kinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="562"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="42"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="57"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="100"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ85942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="611" mass="66991" checksum="A74B6F2266787853" modified="2010-06-15" version="1">MSSSTFSTTPVATPYSHNQPTKLKKSVEDEPSGGACATNPFAAAITTTTSTLASSAPQTFSPPSTTPATNNSAPYPIFKTPALHLADDLPVPGSPKQHKQPQSPSKPSFFLKSPTSFSTHRNSILSVGSVPRQTLLKALASGRPSLDPATLTASLDNVMTSPFPRSASYSPLPNEEGNFTEYHDAMESIGSAPNTAKLLPALQSPCFYHQRFDNAVNVERVLEEIAGDEYTSHSRLVQTALGVREVARQLQRHNCKMNVKNIMIVTKARDNQLVNLTRELAHWIMKTPRYGSKLGVNVYVDKKLKESKRFDAAGLLEEEPKFKDMLRYWTPDLCCVSPETFDLVLTLGGDGTVLFTSWLFQRVVPPILSFSLGSLGFLTNFQFESYKEHLNKVLAEGMRVNMRMRFTCTVYRDEGNGQMSEGDQFEVLNELVIDRGPSPLTVASSLPLLACSTAYSLSAGGSLVHPDIPAILLTPICPHTLSFRPMLLNDSMLLRVSVPKSSRATAWCAFDGKGRVELKQGDHVTIAASQYPFPTVLSAPNEWIDSVSRTLRWNTRAAQQKAWEGDSTSSDGPNLVEEWDIDTDSGVYDSGSDETGKSSPLRRQMSLLNFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>D5GND9</accession>
    <name>D5GND9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DNA-directed RNA polymerase</fullName>
            <ecNumber evidence="11">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00011240001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the phage and mitochondrial RNA polymerase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="11"/>
    <dbReference type="EMBL" id="FN430363">
        <property type="protein sequence ID" value="CAZ86032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841841.1">
        <property type="nucleotide sequence ID" value="XM_002841795.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5GND9">
        <property type="interactions" value="227"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GND9"/>
    <dbReference type="GeneID" id="9182232"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011240001"/>
    <dbReference type="eggNOG" id="KOG1038">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003364_1_0_1"/>
    <dbReference type="InParanoid" id="D5GND9"/>
    <dbReference type="OMA" id="KWFEVDM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034245">
        <property type="term" value="C:mitochondrial DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001018">
        <property type="term" value="F:mitochondrial promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006390">
        <property type="term" value="P:mitochondrial transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000041">
        <property type="entry name" value="DNA-directed RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.280:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1320.10">
        <property type="entry name" value="DNA-directed RNA polymerase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024075">
        <property type="entry name" value="DNA-dir_RNA_pol_helix_hairp_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046950">
        <property type="entry name" value="DNA-dir_Rpol_C_phage-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002092">
        <property type="entry name" value="DNA-dir_Rpol_phage-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037159">
        <property type="entry name" value="RNA_POL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029262">
        <property type="entry name" value="RPOL_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10102">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10102:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00940">
        <property type="entry name" value="RNA_pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14700">
        <property type="entry name" value="RPOL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01311">
        <property type="entry name" value="RPOL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00900">
        <property type="entry name" value="RNA_POL_PHAGE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00489">
        <property type="entry name" value="RNA_POL_PHAGE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 11" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase N-terminal" evidence="13">
        <location>
            <begin position="354"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1333"/>
            <end position="1375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ86032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1416" mass="159215" checksum="B3D262908BB7E697" modified="2010-06-15" version="1">MLRAVGRGIRRQIVRRSSGVRLTSGAPRIPWLCPSPYVYHKPIRWSSSNARAISLPSETPPKHTDRSYSTAAAALELEDIDTSAIFPPLRRDAAPYSIPIEPLEPLDPSQLLVFEPPAAPEEKCMRKSTAGVSSDKAEVLATFNACIKVGVLARAQLMLEQITKNCIEERDTVVLVDAHNIFLKALLDQYSDSGNLSELKVFFMWFENKMRVEYSIKGDATTFALLLKGSLNIPAAEASKRYLAEYINLWRYSGRNIVDVLESAVLNNEEVLKIAKICHLRIGELSATCREMMKEQRKSRLEPPILAVPDAKPTPVKGPGLAAVKSSLRSLIDPDHMPFQEYDINLNDKEFQIERQKLLEEHAFDSARERWKRDFESLSERGVIKQDAEINSLLWEWHQALVPLIHEELKRVQHAEESGTKAAGAVDRCFYGPFMRVIEPEKLAAITIIELLRTHTLNGVGAGLKASRAVLQLGREVELEYQAQEVAKQRGEGFFANMSKAELETIFRDRSAFRNLISKSKSASDLDFEEIPEWPNTVKVKLGGVLISMLIHVARIPVEAAVPGLTEGTESSEKIVQHLPAFQHCYEYQRGRKLGVVKLHPELVKRLGTDDLNFTAIGKSLPMVVRPLPWTDWDEGGYFYTRSKVVRIRESVEQRQYVKAAAERGHLDHLFAGLDVLGRTAWRINRRVFDVVLEVWNQEKAFAKIPALNPDINFPLEPEPNADPKVRWEWAREMKRVNTARKNNHSTRCDVNFKVEIARAFLFEEFYFPHNIDFRGRAYPIPPNLNHIGNDLSRGLLMFSEAKEVGETGLRWMKIHLANLAGYDKASFSQRVAFTEENIENIHDSAENPLTGNRWWLKSDDPWQLLASCFALNEALSLNNPHEYKCHIPVAQDGTCNGLQHYAALGGDVAGARQVNLEPSDLPQDVYTGVAELVKASVELDAKRGLPAAVALKDLVTRKVVKQTVMTNVYGVTFIGARNQIENQLKCIESLDQKDIPALSLYLTKKVFDSIQEMFTGASAIQDWLVTCAKRISRSVRPNQLEHLKDPKTGEDSVHFMTSVVWTTPLRLPVVQPYRQVASAAISTNLQTVYINDPYVIDKVDSRKQMTAFPPNFIHSLDASHMLLSALNCDASGLTFASVHDSFWTHPSDVDEMNRILRDSFVALHGKDIIENLKAEFEERYKGFRTLVKVIANTPCGKRIKLARRNYAIENLGRPKLTAVEDLQWELKRWALMQSHDHDDRKIAAEMVTPSVILEKYGGLKVNELAENTGKLGLSVNDYGSPSLGDQSGDEGSIVDQEDILGPAEESDDGYEESAPLDEDMVLGGMEEGSRMLGTAGDEDIDLDDASTKKRSRARKELDEDGNTIPRDNGSAKGTLKPKQAHIATLNVWVELKFPDLPPKGLFDVNLLRKSEYFFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNN3</accession>
    <name>D5GNN3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00011385001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL24 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430366">
        <property type="protein sequence ID" value="CAZ86126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841935.1">
        <property type="nucleotide sequence ID" value="XM_002841889.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNN3"/>
    <dbReference type="FunCoup" id="D5GNN3">
        <property type="interactions" value="141"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GNN3"/>
    <dbReference type="GeneID" id="9186202"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011385001"/>
    <dbReference type="eggNOG" id="ENOG502QUDZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041333_0_0_1"/>
    <dbReference type="InParanoid" id="D5GNN3"/>
    <dbReference type="OMA" id="TRHDPEY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06089">
        <property type="entry name" value="KOW_RPL26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003256">
        <property type="entry name" value="Ribosomal_uL24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041988">
        <property type="entry name" value="Ribosomal_uL24_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12903">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22682">
        <property type="entry name" value="Ribosomal_uL24m-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="44"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="334"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="45"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ86126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="361" mass="40952" checksum="63FB469ADEC630AC" modified="2010-06-15" version="1">MLKATSRAIRARSQAARKLARVREHNKRMRIKEAMGVRMQIQRIAHADQKQSRREAKEDRMLGPLGPRRAMTSAEADEMSVVATERARPLPIRKEERVKYWNIVPGDRVVVLAGVDRHKIGVVKSVDKATATLMVQGINMAPMKIPDAVFELDTKQQRIYHSELSISYHDVRLVYPLPDPATGALRDTIIANIEMSNIWHNKNGTSSWKRLVPGMEGVVIPWPPKEKPKYVDHPGDTRIMDVEMPSYFPTLLAPPFPLEVIDELRNKYSKFRTRHDPVWVAKKEAEDAAQKAKDGERILTAVQELNRKLRKERKALGPPQLSDSNLEYIGRVMAQNRPELLENLPPTSPPPPTSSTQEATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNQ0</accession>
    <name>D5GNQ0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00011422001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SGT family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430367">
        <property type="protein sequence ID" value="CAZ86147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841956.1">
        <property type="nucleotide sequence ID" value="XM_002841910.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNQ0"/>
    <dbReference type="STRING" id="656061.D5GNQ0"/>
    <dbReference type="GeneID" id="9182782"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011422001"/>
    <dbReference type="eggNOG" id="KOG0553">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044224_1_1_1"/>
    <dbReference type="InParanoid" id="D5GNQ0"/>
    <dbReference type="OMA" id="LAIKDCH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072380">
        <property type="term" value="C:TRC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006620">
        <property type="term" value="P:post-translational protein targeting to endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000207">
        <property type="entry name" value="Small glutamine-rich tetratricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.100:FF:000011">
        <property type="entry name" value="TPR Domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047150">
        <property type="entry name" value="SGT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45831">
        <property type="entry name" value="LD24721P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45831:SF2">
        <property type="entry name" value="LD24721P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 4" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="4">
        <location>
            <begin position="58"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="112"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="197"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="234"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ86147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="256" mass="25908" checksum="28178EA275B3367A" modified="2010-06-15" version="1">MAKKDYSSAIEYYTQALRIVPNNPIYLSNRAAAYSSAAQFEKAILDAQVAVDTDPSYAKAWSRLGHARFAKGDARGAMEAYKAGIEAEGNGGSQITRNGYETAKKKVEEEIAADADNVTAPPGPSTPRGGGVGGMPDLSSIAGMLGGAGRPAGGGMPDLGSIMNNPMFAQMAQNLMSNPSVMQNMMNNPLVREMLDSVGGDDGEGPGAEGRSMPDLSGLLADPSIAEMAKNLMGGPGGGGGGTSGDAGGAGGSGRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNY2</accession>
    <name>D5GNY2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00011582001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ubiquitin family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430371">
        <property type="protein sequence ID" value="CAZ86225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842034.1">
        <property type="nucleotide sequence ID" value="XM_002841988.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNY2"/>
    <dbReference type="STRING" id="656061.D5GNY2"/>
    <dbReference type="GeneID" id="9183518"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011582001"/>
    <dbReference type="eggNOG" id="KOG0001">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010412_7_0_1"/>
    <dbReference type="InParanoid" id="D5GNY2"/>
    <dbReference type="OMA" id="NIQKESX"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd01803">
        <property type="entry name" value="Ubl_ubiquitin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000158">
        <property type="entry name" value="Polyubiquitin 5"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000004">
        <property type="entry name" value="Polyubiquitin Ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019954">
        <property type="entry name" value="Ubiquitin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019956">
        <property type="entry name" value="Ubiquitin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050158">
        <property type="entry name" value="Ubiquitin_ubiquitin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10666">
        <property type="entry name" value="UBIQUITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00348">
        <property type="entry name" value="UBIQUITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00299">
        <property type="entry name" value="UBIQUITIN_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="8">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="8">
        <location>
            <begin position="77"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="8">
        <location>
            <begin position="153"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="8">
        <location>
            <begin position="229"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ86225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="305" mass="34214" checksum="FEA18639BE7EA8FA" modified="2010-06-15" version="1">MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKGKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKGKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKGKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNY3</accession>
    <name>D5GNY3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00011583001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lysine N-acyltransferase MbtK family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430371">
        <property type="protein sequence ID" value="CAZ86226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842035.1">
        <property type="nucleotide sequence ID" value="XM_002841989.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNY3"/>
    <dbReference type="STRING" id="656061.D5GNY3"/>
    <dbReference type="GeneID" id="9183519"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011583001"/>
    <dbReference type="eggNOG" id="ENOG502RZMI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027095_1_0_1"/>
    <dbReference type="InParanoid" id="D5GNY3"/>
    <dbReference type="OMA" id="ITIHTRV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016410">
        <property type="term" value="F:N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019290">
        <property type="term" value="P:siderophore biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000256">
        <property type="entry name" value="Putative lysine N-acyltransferase C17G9.06c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019432">
        <property type="entry name" value="Acyltransferase_MbtK/IucB-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31438">
        <property type="entry name" value="LYSINE N-ACYLTRANSFERASE C17G9.06C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31438:SF1">
        <property type="entry name" value="LYSINE N-ACYLTRANSFERASE C17G9.06C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13523">
        <property type="entry name" value="Acetyltransf_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01006">
        <property type="entry name" value="AlcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Acyltransferase MbtK/IucB-like conserved" evidence="6">
        <location>
            <begin position="253"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="30"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ86226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="423" mass="48991" checksum="FA1F30FEF194F08D" modified="2010-06-15" version="1">MSSDIITIHTRVSSDLHTPRPTEPIRPLFEDNESESELEEPPQSIVEKLHLPNRIALHVTTDGRDDCKVDFECNDLKWGIEISSSSTYPTLHDDVLSFRYYVNEDNQEEYALFKVWVMLFWYFQLPAPSADLIPNKQGSPRGSWQVHFNNTGPFFKLANLQAVEAAGLVSFPDSGAASGEWIGAYATRVGFWQLPPEPFLNPVCTSKKPLHMPKPVEYTFTDRVRHPVRPRPQPNGDMYTRWIPSFGQFLTFRLTDLDEDTEVVHRWMNDPRVSMFWGEEAEDPEKTRSFLKKGLGSKHFFPIIGCWDDEPFGYFEVYWVKEDALGRYASDVDMWDRGVHCLVGEQKFRGPHRVGVWISGLVHFMFLDDPRTQTVMLEPRVDNTKFIKYLVDAGFYKEKEFAFPHKQAALMKLKREAWEGPLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GP77</accession>
    <name>D5GP77_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00011742001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430373">
        <property type="protein sequence ID" value="CAZ86342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842151.1">
        <property type="nucleotide sequence ID" value="XM_002842105.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GP77"/>
    <dbReference type="GeneID" id="9186582"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011742001"/>
    <dbReference type="HOGENOM" id="CLU_3360024_0_0_1"/>
    <dbReference type="InParanoid" id="D5GP77"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ86342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="36" mass="4180" checksum="B0533CAFE341CCC0" modified="2010-06-15" version="1">MPRPLVSPRRSGVRFFHHPSTLLALLDHILPQQQHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPI1</accession>
    <name>D5GPI1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Isocitrate dehydrogenase [NADP]</fullName>
            <ecNumber evidence="11">1.1.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">GSTUM_00011853001</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>D-threo-isocitrate + NADP(+) = 2-oxoglutarate + CO2 + NADPH</text>
            <dbReference type="Rhea" id="RHEA:19629"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11 14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="11 14">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="11 14">Binds 1 Mg(2+) or Mn(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.42" evidence="11"/>
    <dbReference type="EMBL" id="FN430375">
        <property type="protein sequence ID" value="CAZ86424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842233.1">
        <property type="nucleotide sequence ID" value="XM_002842187.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPI1"/>
    <dbReference type="FunCoup" id="D5GPI1">
        <property type="interactions" value="772"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GPI1"/>
    <dbReference type="GeneID" id="9186664"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011853001"/>
    <dbReference type="eggNOG" id="KOG1526">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023296_1_1_1"/>
    <dbReference type="InParanoid" id="D5GPI1"/>
    <dbReference type="OMA" id="HGTVQRH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004450">
        <property type="term" value="F:isocitrate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006097">
        <property type="term" value="P:glyoxylate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006739">
        <property type="term" value="P:NADP+ metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.718.10:FF:000002">
        <property type="entry name" value="Isocitrate dehydrogenase [NADP]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004790">
        <property type="entry name" value="Isocitrate_DH_NADP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00127">
        <property type="entry name" value="nadp_idh_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006156">
        <property type="entry name" value="PRK08299.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11822:SF21">
        <property type="entry name" value="ISOCITRATE DEHYDROGENASE [NADP], MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11822">
        <property type="entry name" value="NADP-SPECIFIC ISOCITRATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000108">
        <property type="entry name" value="IDH_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0329">Glyoxylate bypass</keyword>
    <keyword evidence="6 11 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 11 14" id="KW-0464">Manganese</keyword>
    <keyword evidence="5 11 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11 15" id="KW-0521">NADP</keyword>
    <keyword evidence="8 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="16">
        <location>
            <begin position="76"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="142"/>
            <end position="144"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="161"/>
            <end position="167"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="317"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="325"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="340"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="375"/>
            <end position="380"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="393"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="site" description="Critical for catalysis" evidence="12">
        <location>
            <position position="206"/>
        </location>
    </feature>
    <feature type="site" description="Critical for catalysis" evidence="12">
        <location>
            <position position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR000108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000108-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAZ86424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="478" mass="53476" checksum="98B57D3EF19E727A" modified="2010-06-15" version="1">MPLGATTSRCLLLRSRAIASTATIVARPPLLQCSTRVGKKENKAGSGIRLERLGNLHAFSVSMERQEFQKIKVKNPIVELDGDEMTRVIWDWIKARFIHPYLDVDLKYYDLGILYRDKTEDKVTVEAAEAILKHSVGVKCATITPDEARVKEFNLKKMWLSPNGTIRNILGGTVFREPIVIPRIPRLVAGWKEPIIIGRHAHGDQYKAQDFVASGPGKLEMVYTPENGEPKRIQVFDYKASGVALAMYNTDDSISGFAHASFKLALQKKLPLYMSTKNTILKKYDGRFKDIFQNIYESTYKTEFDAKGIWYEHRLIDDMVAQMIKSKGGFIMALKNYDGDVQSDIVAQGFGSLGLMTSVLITPDGKTFESEAAHGTVTRHYREYQKGKETSTNPIASIFAWTRGLIQRGTLDNTPDVVAFAESLESAIIDTVQVDGIMTKDLALACGKQDRDSWVTTSIFLDGVEKRFKGSLKAKSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPM8</accession>
    <name>D5GPM8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00011938001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430377">
        <property type="protein sequence ID" value="CAZ86471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842280.1">
        <property type="nucleotide sequence ID" value="XM_002842234.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPM8"/>
    <dbReference type="GeneID" id="9183577"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011938001"/>
    <dbReference type="HOGENOM" id="CLU_2499514_0_0_1"/>
    <dbReference type="InParanoid" id="D5GPM8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ86471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="86" mass="9511" checksum="F18E686A8B9C8EC6" modified="2010-06-15" version="1">MPRGGLGNMPETGIPAYWGYCFTPSSSIELKHADKLPLLQVSGDFAEAWTSQSFLTFPFRPHTISAKPGLSLRGERKIGKVERMTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPT8</accession>
    <name>D5GPT8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00012028001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430378">
        <property type="protein sequence ID" value="CAZ86531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842340.1">
        <property type="nucleotide sequence ID" value="XM_002842294.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPT8"/>
    <dbReference type="GeneID" id="9182275"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012028001"/>
    <dbReference type="HOGENOM" id="CLU_883369_0_0_1"/>
    <dbReference type="InParanoid" id="D5GPT8"/>
    <dbReference type="OMA" id="SHAFCAF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="258"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="10"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="182"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="264"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ86531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="315" mass="33896" checksum="A7761D972A54C7B3" modified="2010-06-15" version="1">MADFFPPFTSSPGRASLLGLGLPPLSSAENSPPAALRSAPSSRFHREQRKALHSAPDESDDGCSPHPSIYEASPVASDEECQSDKEPLSVCFETAKPQAADLLSSNPDGTYSPPGGRDISRFSGLSLLETITEQKSASTQPEEGGQRSYTQVPTEDDDESEDEELEQYYEYASPTQPLHPALSTSAPQYTESPYPSPSQSHPTSAPASNSFFTRGLPPSPRPFPHQPNFRPAVSMNRSYGTLSSHPFHRAPVLATFPGGYPTDNMGTPDSLQSPSSRSRAGSLQEGGIWTMISHAFCAFCCCVSVGDSERWGEHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPU2</accession>
    <name>D5GPU2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00012032001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430378">
        <property type="protein sequence ID" value="CAZ86535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842344.1">
        <property type="nucleotide sequence ID" value="XM_002842298.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPU2"/>
    <dbReference type="STRING" id="656061.D5GPU2"/>
    <dbReference type="GeneID" id="9182279"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012032001"/>
    <dbReference type="HOGENOM" id="CLU_128881_0_0_1"/>
    <dbReference type="InParanoid" id="D5GPU2"/>
    <dbReference type="OMA" id="NPGADMD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033615">
        <property type="term" value="P:mitochondrial proton-transporting ATP synthase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039196">
        <property type="entry name" value="Fmc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28015">
        <property type="entry name" value="ATP SYNTHASE ASSEMBLY FACTOR FMC1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28015:SF1">
        <property type="entry name" value="ATP SYNTHASE ASSEMBLY FACTOR FMC1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13233">
        <property type="entry name" value="Complex1_LYR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ86535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="99" mass="10934" checksum="1DF71AC9D1711435" modified="2010-06-15" version="1">MATTTTTTKTLYRSLLRELPPISQKRTHLHHRLRGSISGKGEGDGGMLLLAQLTTYLRAQRTYSALLERYNPSLGEGSGGGDDDRVRMSARRVGLHVPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPW8</accession>
    <name>D5GPW8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00012082001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430379">
        <property type="protein sequence ID" value="CAZ86570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842379.1">
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    <dbReference type="FunCoup" id="D5GPW8">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GPW8"/>
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    <dbReference type="KEGG" id="tml:GSTUM_00012082001"/>
    <dbReference type="eggNOG" id="ENOG502QURG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033465_3_1_1"/>
    <dbReference type="InParanoid" id="D5GPW8"/>
    <dbReference type="OMA" id="IGGFFEW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007568">
        <property type="entry name" value="RTA1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31465">
        <property type="entry name" value="PROTEIN RTA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31465:SF35">
        <property type="entry name" value="RTA1 DOMAIN PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04479">
        <property type="entry name" value="RTA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="102"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="140"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="183"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="218"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ86570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="264" mass="29549" checksum="04384D9E34ECB24D" modified="2010-06-15" version="1">MSDNTSENFELYRYTPSLVGAVIFAALFFISSGYHVIQLTRARTWYFIPLVIGALMEAVLAPYLVQALLLLVAPALYAASIYMILGRLITSIEAERHSLIRTTWLTKIFVAGDVLSFLMQSTGGGMMAKGDDSVKMGEHIVLGGLFVQILFFSLFMIATVVFHLRIRKDPTQASQMGTAWRTLLMALYIASLSILVRSLFRIVEYIQGREGYLMSNEVWLYIFDAVLMLSVTVLFNVVHPSVAVPGRRRKVTEHGYAMRSGDLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPY5</accession>
    <name>D5GPY5_TUBMM</name>
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        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00012093001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430380">
        <property type="protein sequence ID" value="CAZ86578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842387.1">
        <property type="nucleotide sequence ID" value="XM_002842341.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPY5"/>
    <dbReference type="GeneID" id="9182322"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012093001"/>
    <dbReference type="HOGENOM" id="CLU_2529115_0_0_1"/>
    <dbReference type="InParanoid" id="D5GPY5"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ86578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="84" mass="9474" checksum="8B433EC0DED07120" modified="2010-06-15" version="1">MKRTYGRSIHTTAPLYAFSFQILPWRSHFEQLYGVKGFTTIPVSPQSSYMAYLYGTGYVFPPPPIAPSHPLRPSPSNSLTSCFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5GQ44</accession>
    <name>D5GQ44_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00012193001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430382">
        <property type="protein sequence ID" value="CAZ86637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842446.1">
        <property type="nucleotide sequence ID" value="XM_002842400.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GQ44"/>
    <dbReference type="GeneID" id="9185217"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012193001"/>
    <dbReference type="eggNOG" id="KOG2922">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011406_1_0_1"/>
    <dbReference type="InParanoid" id="D5GQ44"/>
    <dbReference type="OMA" id="PLCFLIY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3730.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008521">
        <property type="entry name" value="Mg_trans_NIPA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570:SF86">
        <property type="entry name" value="ADR321CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05653">
        <property type="entry name" value="Mg_trans_NIPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="152"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="230"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="262"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="288"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="337"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="389"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="462"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="354"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="398"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ86637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="487" mass="52589" checksum="120605912A1A7ABB" modified="2010-06-15" version="1">MVGLGDLSSGAQITIGVCVGVLSTSVQSLGLTLQRKSHLLEDDRPPHIKKRPPHRRRMWQIGILLFIVSNILGSSIQITTLPLVILSPLQASGLVFNSICATLILSEPFTRYSLIGTILVCIGAALIAAFGAMKEPAHSLDELLDLLAKKTFLSWMACTALLVFGILGATKASSILRPRLKHTAKMRMVRGVAYGCVSGILSAHCLLLAKSAVELLVRTIVDRHNQFNRWQSWMILLGLAALALTQLYYLHRGLKLCSTSVLYPLVFCVYNIIAILDGLLYYRQASRLTLLHGCLIALGTAILLTGVLALSWRLNQEQTGPSAGAAGALSPGIGFIDTDSSDPSTDTEGESSEIDTNQQLLSPSSPSARSKFLKRRRALSEVEEIWGELQDEGGLPRDEEEAGSDVDEGTSLLRKGPGGRRYTRKGSYIGRGGSGRSSRKERILQGAIGGWWKLKWWKEGKEGNPGCRRGQSDDGDDTQDGGGGGGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5G3W4</accession>
    <name>D5G3W4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glycogen [starch] synthase</fullName>
            <ecNumber evidence="8">2.4.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00003828001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Glycogen synthase participates in the glycogen biosynthetic process along with glycogenin and glycogen branching enzyme. Extends the primer composed of a few glucose units formed by glycogenin by adding new glucose units to it. In this context, glycogen synthase transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.</text>
    </comment>
    <comment type="function">
        <text evidence="8">Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>[(1-&gt;4)-alpha-D-glucosyl](n) + UDP-alpha-D-glucose = [(1-&gt;4)-alpha-D-glucosyl](n+1) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18549"/>
            <dbReference type="Rhea" id="RHEA-COMP:9584"/>
            <dbReference type="Rhea" id="RHEA-COMP:9587"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15444"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.11"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:18550"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Glycan biosynthesis; glycogen biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the glycosyltransferase 3 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.11" evidence="8"/>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835086.1">
        <property type="nucleotide sequence ID" value="XM_002835040.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G3W4"/>
    <dbReference type="FunCoup" id="D5G3W4">
        <property type="interactions" value="403"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G3W4"/>
    <dbReference type="GeneID" id="9187145"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003828001"/>
    <dbReference type="eggNOG" id="KOG3742">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015910_1_0_1"/>
    <dbReference type="InParanoid" id="D5G3W4"/>
    <dbReference type="OMA" id="RDVRNHI"/>
    <dbReference type="UniPathway" id="UPA00164"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004373">
        <property type="term" value="F:alpha-1,4-glucan glucosyltransferase (UDP-glucose donor) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03793">
        <property type="entry name" value="GT3_GSY2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000014">
        <property type="entry name" value="Glycogen [starch] synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000045">
        <property type="entry name" value="Glycogen [starch] synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008631">
        <property type="entry name" value="Glycogen_synth"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10176:SF3">
        <property type="entry name" value="GLYCOGEN [STARCH] SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10176">
        <property type="entry name" value="GLYCOGEN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05693">
        <property type="entry name" value="Glycogen_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0320">Glycogen biosynthesis</keyword>
    <keyword evidence="3 8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="618"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="690"/>
            <end position="702"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00043883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ79207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="702" mass="79861" checksum="DFDFD16353DC12DA" modified="2010-06-15" version="1">MSEAPEGRRDVKNHLLFEISTEVANRVGGIYSVIKSKAPITTAEYGERYCLIGPLNRTSAAVEVEALEPTNPALKATLAAMSDRGIQWLYGRWLIDGAPRVLLFDTGTGYKHLDEWKGDLWSTAGIPCPPGDAETNEAVVFGYLVAWFLGEFVSHEQSRAVIAHFHEWLAGVALPLCRKRRIDVTTIFTTHATLLGRYLCAGSVDFYNNLQYFDVDAEAGKRGIYHRYCIERAATHSCDVFTTVSHITAYESEHLLKRKPDGVLPNGLNVVKFSAMHEFQNLHAQSKEKIHDFIRGHFYGHNDFDLENTLYFFTAGRYEYRNKGVDMFIESLARLNHRLKISGSKTTVVAFIIMPAQTHSLTVEALKGQAVIKALRDTVDNIEKGIGKRLFERALRWHDGEQVPAEKDLMSNQEKVMLKRRVYALKRNALPPIVTHNMIDDQADPILNQIRRVQLFNHPSDRVKIVFHPEFLNSSNPVLSLDYDDFVRGTHLGVFASYYEPWGYTPAECTVMGVPSITTNLSGFGCYMEELIENASDYGIYIVDRRMKGVDDSVNQLCQFMYDFTVKSRRQRINQRNRTERLSDLLDWKRMGMEYVKARQLALRRAYPGSFDSKEPFPFFDGTEQKVSRPLSAPGSPRDRSGMMTPGDFPSLQEGMEGLNTDDYVGWRLPEEEDPDDYHFPLTLRAPLTKRQQPSVNGEHNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4A3</accession>
    <name>D5G4A3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00004019001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CBF/MAK21 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835225.1">
        <property type="nucleotide sequence ID" value="XM_002835179.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4A3"/>
    <dbReference type="FunCoup" id="D5G4A3">
        <property type="interactions" value="585"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G4A3"/>
    <dbReference type="GeneID" id="9184971"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004019001"/>
    <dbReference type="eggNOG" id="KOG2154">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015945_1_0_1"/>
    <dbReference type="InParanoid" id="D5G4A3"/>
    <dbReference type="OMA" id="YYNNIVT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030692">
        <property type="term" value="C:Noc4p-Nop14p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005612">
        <property type="entry name" value="CCAAT-binding_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027193">
        <property type="entry name" value="Noc4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12455">
        <property type="entry name" value="NUCLEOLAR COMPLEX PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12455:SF0">
        <property type="entry name" value="NUCLEOLAR COMPLEX PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03914">
        <property type="entry name" value="CBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="CCAAT-binding factor" evidence="5">
        <location>
            <begin position="338"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ79346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="571" mass="64926" checksum="85C8309F55DA9CC2" modified="2010-06-15" version="1">MAPKHPKARVEIAGKLSADRKERSRKLALLKKKRTELVKQTSKLQHEQTTDEIEGLEKEIVASPRGYNHVVKLLEHLKSPEKTTISAAATSLCRAFIKLMVKGELSKKKGATDAEVQILEWLRGQYGIFIGDLCGLLDYKDGGIQEEVLWLLMRLVKAESEHLTPPEEEAYFPHKLFRRIVEALVYAENLEDYLRIQFTEKYLDVYDDIRYSFFSAVKTLTQIALKTPSTLPQFTKTTITLILSLHNLPTSTTPPFTSFFALIPPSSTSPTNPRAILALTPHRKLLQTVLLHLLRLPTITPYTPSILQNITTLAPTFPNPETLMDFLTDTYATGGPTALLALSGLFYLIKTKNLDYQNFYQNLYALLDRNLLHLKYRSRFFRLLEEFLGSKYLPAALIASFLKRLARLALSAPPAAVVVIIPFVYNLLKAHPACWFMLHREGTTEELDRWRKDGVVDPFDPEEEDPLETGAIDSCLWELETLRGHWQPNVATLARILGEQFTKASYGLEDFLDHSYGSMFAAEMKKNVNSERPPVVEFEVPKRIFFEVEREGGNEDLSEAAANPLLSLWEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4I8</accession>
    <name>D5G4I8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00004131001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429987">
        <property type="protein sequence ID" value="CAZ79431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842511.1">
        <property type="nucleotide sequence ID" value="XM_002842465.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4I8"/>
    <dbReference type="GeneID" id="9184060"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004131001"/>
    <dbReference type="HOGENOM" id="CLU_2098610_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4I8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ79431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="116" mass="13239" checksum="E156E6F6C25D7127" modified="2010-06-15" version="1">MVFFAGNIAMANQRIELKPWHIRLESSISNRPGGFDSGIIPTIAWHMVMFLPNPLPLRGKESPEEGYSTPSLSRPTPAKCPISPFRFFCDPLSYRKDPVRDYRVSRFPRDLSSLET</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4J1</accession>
    <name>D5G4J1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00004171001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429987">
        <property type="protein sequence ID" value="CAZ79434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842514.1">
        <property type="nucleotide sequence ID" value="XM_002842468.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4J1"/>
    <dbReference type="GeneID" id="9184066"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004171001"/>
    <dbReference type="eggNOG" id="ENOG502S56Q">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065618_2_2_1"/>
    <dbReference type="InParanoid" id="D5G4J1"/>
    <dbReference type="OMA" id="ITWQAKP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018466">
        <property type="entry name" value="Kre9/Knh1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052982">
        <property type="entry name" value="SRP1/TIP1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40633:SF1">
        <property type="entry name" value="GPI ANCHORED SERINE-THREONINE RICH PROTEIN (AFU_ORTHOLOGUE AFUA_1G03630)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40633">
        <property type="entry name" value="MATRIX PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_8G05410)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10342">
        <property type="entry name" value="Kre9_KNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003071987" evidence="2">
        <location>
            <begin position="19"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="Yeast cell wall synthesis Kre9/Knh1-like N-terminal" evidence="3">
        <location>
            <begin position="34"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ79434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="280" mass="28726" checksum="EA0CAE83DF7CD757" modified="2010-06-15" version="1">MKYALFTVLAAAFTFANAYTTPVGDTTEGNPISKPGLGEIVPAGSPYTITWNPTTGGTVTLLLLRGPSNNVVPLYPIVEMAPNTGTYTWTPATDLEADVSRYGIQLIDDATGKYQYTTQFGVSNSNPYMGSSSSSAPPLYPLSVPPTSSVSTSCTSSLVPTHSVPTYAVSSGYPTAIGGLVTSGTVLYSTSMVTVTSCGPTVTNCPGKPYTTPAAVWTSPAWNGTATKLANPTGYPSAIPSTNVTKPSIPVIPVSPGAGDKVRVGGVMLVVMVAGLALLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5G528</accession>
    <name>D5G528_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glucosidase 2 subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00000285001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429996">
        <property type="protein sequence ID" value="CAZ79659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835502.1">
        <property type="nucleotide sequence ID" value="XM_002835456.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G528"/>
    <dbReference type="FunCoup" id="D5G528">
        <property type="interactions" value="518"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G528"/>
    <dbReference type="GeneID" id="9186357"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000285001"/>
    <dbReference type="eggNOG" id="KOG2397">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016834_2_1_1"/>
    <dbReference type="InParanoid" id="D5G528"/>
    <dbReference type="OMA" id="YENGQHC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017177">
        <property type="term" value="C:glucosidase II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006491">
        <property type="term" value="P:N-glycan processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.130.10">
        <property type="entry name" value="Mannose-6-phosphate receptor binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039794">
        <property type="entry name" value="Gtb1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009011">
        <property type="entry name" value="Man6P_isomerase_rcpt-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044865">
        <property type="entry name" value="MRH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036607">
        <property type="entry name" value="PRKCSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028146">
        <property type="entry name" value="PRKCSH_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12630:SF1">
        <property type="entry name" value="GLUCOSIDASE 2 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12630">
        <property type="entry name" value="N-LINKED OLIGOSACCHARIDE PROCESSING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12999">
        <property type="entry name" value="PRKCSH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13015">
        <property type="entry name" value="PRKCSH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50911">
        <property type="entry name" value="Mannose 6-phosphate receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51914">
        <property type="entry name" value="MRH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072524" description="Glucosidase 2 subunit beta" evidence="6">
        <location>
            <begin position="19"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="MRH" evidence="7">
        <location>
            <begin position="383"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="177"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ79659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="521" mass="57848" checksum="ED4301118CB8E1A8" modified="2010-06-15" version="1">MRRGSILLLLSSASIATASSSPRGVSPEDANLYTSCGEKTFTCLTNPHVTIPYENVNDDYCDCPDGSDEPGTSSCSHLPHKSLAIRGFYCKNEKHTPAFLPLSRVNDGICDYEICCDGSDEWAGVGGVKCENKCGEIGKAAGKLAAEKGRLRDEGVRKKKELLGRAKTMRIELEYNVKTTRVRIEALEGKVRRLEVQLRETEEEEKLKMAKQPKEGSKLGILISLARERMQELKSSLERVRDDRDSARSKLEKAEGILKALKEGYNPNFNDEGVKAARDLNSLLDEDEIDWEGFTEPDDVAEGGIRTWVHDKLADFRQMLVENGLLAAKSDDGMPESRALASARQALETGRTEESDTKSHLETLLSDLTYPYGQDDVFRPLKGECISSQFGEYTYEYCFLGTAYQKSLKDSSSVSLGEYSRIEVDKSINDASVRGIFESGWEEAHDEGLSGTSLIHENGQQCWNGPRRSVKVDLYCSAVDELRSVREEEKCVYRFEVGTAAVCGGEDGRGENTEENVKSEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>D5G588</accession>
    <name>D5G588_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00004219001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the ClpA/ClpB family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835524.1">
        <property type="nucleotide sequence ID" value="XM_002835478.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G588"/>
    <dbReference type="FunCoup" id="D5G588">
        <property type="interactions" value="448"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G588"/>
    <dbReference type="GeneID" id="9188100"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004219001"/>
    <dbReference type="eggNOG" id="KOG1051">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005070_4_1_1"/>
    <dbReference type="InParanoid" id="D5G588"/>
    <dbReference type="OMA" id="ERMKAVM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140602">
        <property type="term" value="C:nucleolar peripheral inclusion body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140453">
        <property type="term" value="C:protein aggregate center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051787">
        <property type="term" value="F:misfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070370">
        <property type="term" value="P:cellular heat acclimation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071218">
        <property type="term" value="P:cellular response to misfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043335">
        <property type="term" value="P:protein unfolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19499">
        <property type="entry name" value="RecA-like_ClpB_Hsp104-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000025">
        <property type="entry name" value="ATP-dependent Clp protease subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000010">
        <property type="entry name" value="Chaperone clpB 1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1780.10">
        <property type="entry name" value="Clp, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036628">
        <property type="entry name" value="Clp_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004176">
        <property type="entry name" value="Clp_R_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001270">
        <property type="entry name" value="ClpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018368">
        <property type="entry name" value="ClpA/B_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028299">
        <property type="entry name" value="ClpA/B_CS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041546">
        <property type="entry name" value="ClpA/ClpB_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050130">
        <property type="entry name" value="ClpA_ClpB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11638">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11638:SF18">
        <property type="entry name" value="HEAT SHOCK PROTEIN 104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17871">
        <property type="entry name" value="AAA_lid_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02861">
        <property type="entry name" value="Clp_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00300">
        <property type="entry name" value="CLPPROTEASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81923">
        <property type="entry name" value="Double Clp-N motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51903">
        <property type="entry name" value="CLP_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00870">
        <property type="entry name" value="CLPAB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00871">
        <property type="entry name" value="CLPAB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Clp R" evidence="9">
        <location>
            <begin position="1"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="430"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ79681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="836" mass="91886" checksum="E5F290429C9C2901" modified="2010-06-15" version="1">MSNLQLTDKASKALSDAHESASQYGHPQVTPVHLVCALIDGPQVENDTAPAGAAPASSSPSLLRQVIEKANGDFQTFERAVKKARVRMPSQDPPPDQVSFSPSLSKVLRDAQKLQKTQRDSYIAQDHLISAVAQDSGIQKLMVEAGIPKVSLLDDAVQSLRGTRRVDSQNEESVESENLSKYCVDLTQRARDGKLDPVIGREQEILRVVRILSRRTKNNPVLIGEPGVGKTSVVEGLATRIINADVPANLASCKLLTLDVGSLVAGTKYRGEFEDRMKAVLKEIEDSKQMIVLFIDEIHLLMGAGSSGEGGMDAANLLKPALARGQLHCIGATTLAEYRKYIEKDAAFERRFQQVLCPEPSVPETISILRGLKERYEVHHGVTISDGAIVAAATLASRYLTARRLPDSAIDLIDEAASAVRVTRESAPEIAANCEQAERLEAEKLEAEQDAAISSANGTGENNPMLTDLVGPEQINEIVAKWTGIPVTRLKTTEKEKLLNMEKYLSKQVIGQREAVKAVANAIRLSRSGLSNPNQPIASFLFCGPSGTGKTLLTKSLAEFLFDDPKSMIRFDMSEYMEKHSVSRLIGAPPGYIGHDSGGQLTEALRRRPFSILLFDEIEKAAKEVLTVLLQLLDDGRITDSTGRIVDGKNTVIIMTSNLGSQYISSHSHSEDGRIDPTTRELVNEAIRQHFLPEFINRISSIVIFNRLAKREIRKIVDLRLVDIQKRLRSNNRNVILLVDEQARDYLGEAGYSPSYGARPLNRVIEKEILNKLAVLLLRGAIREGEVAHITMDETGHRIVVLPNHDSDIEEDEDMDYIEEDAQGMGGEDPDIELYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5D9</accession>
    <name>D5G5D9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00004288001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835575.1">
        <property type="nucleotide sequence ID" value="XM_002835529.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5D9"/>
    <dbReference type="STRING" id="656061.D5G5D9"/>
    <dbReference type="GeneID" id="9188151"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004288001"/>
    <dbReference type="HOGENOM" id="CLU_804580_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5D9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059018">
        <property type="entry name" value="HEAT_URB1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26140">
        <property type="entry name" value="HEAT_URB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="URB1 central HEAT repeat" evidence="1">
        <location>
            <begin position="2"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF26140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="345" mass="37657" checksum="213A1CD59C8740D7" modified="2010-06-15" version="1">MPLSPFVSILRACCGSSSRFPPQQIKTLLHSFVAPSLLLQAETPVSPLEALLESLSAIAQPATFEATLVFLDESILRCIRTPFKYIDDYAELVLNISGPQRQGQGTAAVSPLVMTIVEQFKFFIESDAPRGVKLGFSTWLARFMESCAILGENGHALAALCNRLVELCGYDKASKGVFKSLKQDLEGGGGFQLVNRESPEPTPKRYGGIISGEKRSRDLFRSLRRLDTQAIENLLRLYRDVGAEVSLLDIVVICRAVGSILDGSTINEGDACAAISKLLGLLKALFERIPRGDGLRGRAKAMLAHDSIWIESFLHVPVDPGRVPRYIIFSTGNCPLCLLDESKAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5E5</accession>
    <name>D5G5E5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Golgi SNAP receptor complex member 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00004296001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport. It belongs to a super-family of proteins called t-SNAREs or soluble NSF (N-ethylmaleimide-sensitive factor) attachment protein receptor.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of several multiprotein Golgi SNARE complexes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the GOSR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835581.1">
        <property type="nucleotide sequence ID" value="XM_002835535.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5E5"/>
    <dbReference type="FunCoup" id="D5G5E5">
        <property type="interactions" value="849"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G5E5"/>
    <dbReference type="GeneID" id="9188157"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004296001"/>
    <dbReference type="eggNOG" id="KOG3208">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078034_1_0_1"/>
    <dbReference type="InParanoid" id="D5G5E5"/>
    <dbReference type="OMA" id="QAYAVND"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005797">
        <property type="term" value="C:Golgi medial cisterna"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048219">
        <property type="term" value="P:inter-Golgi cisterna vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023601">
        <property type="entry name" value="Golgi_SNAP_su1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21094:SF2">
        <property type="entry name" value="GOLGI SNAP RECEPTOR COMPLEX MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21094">
        <property type="entry name" value="GOS-28 SNARE- RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12352">
        <property type="entry name" value="V-SNARE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF027109">
        <property type="entry name" value="Golgi_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="7 9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="210"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR027109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ79738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="228" mass="25735" checksum="D4E93168646A4B13" modified="2010-06-15" version="1">MTTSTSNSWAQLRQQARSLESQTDNLFHTYSSFVSNPAAKPSEAELRTESQLQEILQKRETVVSSLSRLLDSETALTSSATKLQNLSLHRSTLTDHRHEFVRLKGTVSESRSRTHLLSSVRDDINAFRSASRIEEGRSEADYMLDERDRIDNSHNVADSVLSQAYAIQSDFTDQRQLLGSINRRIVHSASQIPGINTIIAKINTRKKRDSIILAGLIAACFLMVLWFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5Z6</accession>
    <name>D5G5Z6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00001696001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430005">
        <property type="protein sequence ID" value="CAZ79939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5Z6"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001696001"/>
    <dbReference type="HOGENOM" id="CLU_2198906_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5Z6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ79939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="108" mass="11828" checksum="2E6831D3A769E1A6" modified="2010-06-15" version="1">MSGGRGGVLVSFGISVSLSFLPYLICSNSRSNLFVFSCYFFPSLWRGMMGRGGIVSIGLRVDSMEMEKSGCALVRTSCGEGEKNQKKKKKRPESTKTNHLGKRISINP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6N3</accession>
    <name>D5G6N3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00002139001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430012">
        <property type="protein sequence ID" value="CAZ80176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836019.1">
        <property type="nucleotide sequence ID" value="XM_002835973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6N3"/>
    <dbReference type="SMR" id="D5G6N3"/>
    <dbReference type="STRING" id="656061.D5G6N3"/>
    <dbReference type="GeneID" id="9187202"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002139001"/>
    <dbReference type="eggNOG" id="KOG2912">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027534_0_1_1"/>
    <dbReference type="InParanoid" id="D5G6N3"/>
    <dbReference type="OMA" id="EHKIDNY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120048">
        <property type="term" value="F:U6 snRNA (adenine-(43)-N(6))-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120049">
        <property type="term" value="P:snRNA (adenine-N6)-methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010286">
        <property type="entry name" value="METTL16/RlmF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13393:SF0">
        <property type="entry name" value="RNA N6-ADENOSINE-METHYLTRANSFERASE METTL16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13393">
        <property type="entry name" value="SAM-DEPENDENT METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05971">
        <property type="entry name" value="Methyltransf_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ80176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="377" mass="42025" checksum="4FB036541DE35BE6" modified="2010-06-15" version="1">MAPGAVQGLILPRELTKCLLHRDFGLNLALPSDRLCPTVPNRFRLNYVLWIQDLLDSTGNLENDEFDSTREVVGLDVGTGASCIYPLLGCALRDGWKFIATDIDGKSLEYARENVRRNKEKVGDLDERIMVLDNSPDDDIFPVGKLGIEMLDFTMCNPPFYASIDDMLDSARQKDLPPSSACTGTTTEMVCPGGETSFVALMLNQSLQFCSILGFKNTWYTSMLGKRSSVESLVPEIKLHTQNYAITEFKQGSTRRWGVAWSFGDRRPCLAAARYESSQLRGLMPFPTEFEFDAAGDVISVGWGTVKGNVWSRAARRAKARGLEMEGVSKGIVLGFRVGVKEKEDQSVKVRIRWTRGTDSVLFESFCGMIKRKILGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6W0</accession>
    <name>D5G6W0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00002304001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430017">
        <property type="protein sequence ID" value="CAZ80253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836096.1">
        <property type="nucleotide sequence ID" value="XM_002836050.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6W0"/>
    <dbReference type="GeneID" id="9182477"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002304001"/>
    <dbReference type="HOGENOM" id="CLU_1166573_0_0_1"/>
    <dbReference type="InParanoid" id="D5G6W0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="136"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="238" mass="25650" checksum="2A68A67545531B26" modified="2010-06-15" version="1">MLAIQFPSSPIDARTPSPPTSFHADYYTSAPLLAPPPVNKRRAGPPKLSLQITSETRRTFSKSSIPPVSAGPLSPTSLSTQRNTQFNARRDSAPTSFSAAPIEQASSRTNGSNKKRRMGARTVGFAEGVEVIGYSVRWEDKQPQRVEEESSSSSSSEDDEEEVRARKVGGNLRGLEQMEALKLGRGSSRGYNWRMKMLNSRSSIPTTTDEVTEPMSATLGTSTGGVGFGFGSGIGRFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5G769</accession>
    <name>D5G769_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">type I protein arginine methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.319</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00002492001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-arginyl-[protein] + S-adenosyl-L-methionine = N(omega)-methyl-L-arginyl-[protein] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48100"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11990"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65280"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:48101"/>
        </physiologicalReaction>
    </comment>
    <dbReference type="EC" id="2.1.1.319" evidence="1"/>
    <dbReference type="EMBL" id="FN430022">
        <property type="protein sequence ID" value="CAZ80362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836171.1">
        <property type="nucleotide sequence ID" value="XM_002836125.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G769"/>
    <dbReference type="FunCoup" id="D5G769">
        <property type="interactions" value="882"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G769"/>
    <dbReference type="GeneID" id="9185260"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002492001"/>
    <dbReference type="eggNOG" id="KOG1499">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017375_1_2_1"/>
    <dbReference type="InParanoid" id="D5G769"/>
    <dbReference type="OMA" id="CTHTKVK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042054">
        <property type="term" value="F:histone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035242">
        <property type="term" value="F:protein-arginine omega-N asymmetric methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035241">
        <property type="term" value="F:protein-arginine omega-N monomethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060567">
        <property type="term" value="P:negative regulation of termination of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.160.11:FF:000001">
        <property type="entry name" value="Blast:Protein arginine N-methyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000003">
        <property type="entry name" value="Blast:Protein arginine N-methyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.160.11">
        <property type="entry name" value="Hnrnp arginine n-methyltransferase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025799">
        <property type="entry name" value="Arg_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055135">
        <property type="entry name" value="PRMT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006:SF53">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06325">
        <property type="entry name" value="PrmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22528">
        <property type="entry name" value="PRMT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51678">
        <property type="entry name" value="SAM_MT_PRMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein arginine N-methyltransferase" evidence="7">
        <location>
            <begin position="178"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF22528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ80362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="357" mass="40783" checksum="CFE4CF829D3BD215" modified="2010-06-15" version="1">MGEKRLEQAEEHYFQSYNHHGIHEEMLVRDIPLFVPPFTTGKISSYRIFRQKDEVRTNAYKNAIYQNAHLFKDKVVLDVGCGTSILSMFAVKAGAKHVIGVDMSTIIEKASEIVKINGMSDRITLLQGKMEEVVLPVEKVDIIISEWMGYFLLYESMLDTVLLARDKYLAPGGLIFPDKAHIYMAAIEDGDYKEEKIGYWNNVYGFDFTPLQRTALAEPLVDTVEMKAVVTDPGQVFSIDLYTVMAPELAFHVPFELHFNRNDFAHAFIAWFDIEFSACHKPIKFSTGPHAKYTHWKQTVFYLHEVLRVNRGEVITGSISSKPTEKNRRDLDIKIDYQLQTADPSRASAGSCNYKMC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>D5G789</accession>
    <name>D5G789_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00002515001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the V-ATPase e1/e2 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430022">
        <property type="protein sequence ID" value="CAZ80382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836191.1">
        <property type="nucleotide sequence ID" value="XM_002836145.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G789"/>
    <dbReference type="STRING" id="656061.D5G789"/>
    <dbReference type="GeneID" id="9185280"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002515001"/>
    <dbReference type="HOGENOM" id="CLU_221126_0_0_1"/>
    <dbReference type="InParanoid" id="D5G789"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008389">
        <property type="entry name" value="ATPase_V0-cplx_e1/e2_su"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05493">
        <property type="entry name" value="ATP_synt_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ80382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="32" mass="3855" checksum="286D6A844D31CEDD" modified="2010-06-15" version="1">MWAITFMAQLHPLVMPRRSDLRPEAHSDPRFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7D2</accession>
    <name>D5G7D2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00002556001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430024">
        <property type="protein sequence ID" value="CAZ80425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836234.1">
        <property type="nucleotide sequence ID" value="XM_002836188.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7D2"/>
    <dbReference type="STRING" id="656061.D5G7D2"/>
    <dbReference type="GeneID" id="9187254"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002556001"/>
    <dbReference type="eggNOG" id="ENOG502S5EQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037529_0_1_1"/>
    <dbReference type="InParanoid" id="D5G7D2"/>
    <dbReference type="OMA" id="MGMKSHG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39606:SF1">
        <property type="entry name" value="CELL SURFACE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39606">
        <property type="entry name" value="SURFACE PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="148"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="191"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="233" mass="23635" checksum="5873F57674C8088C" modified="2010-06-15" version="1">MSNVTNKVRDAIASQQDDTTTDRTAGPHRSDMANRADPRVESDRGHHGNPVGGAGLGGMGSTHTAGSTNYGPHDSNIANKVDPRVDSDRDGHGLHNASGAHGAMGLGTGYNTSTNVGPDREGRAGLGSTTQGAPGIGSTGTHTGTGYGTSTSHGPHGSNVGNKLDPRVDSDRDHRASQAATSTGPAPDTAGPHKSDLMNKLDPRVDSNLDGSKTIGADKTYTAGSGATRGMHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7J8</accession>
    <name>D5G7J8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00002606001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430029">
        <property type="protein sequence ID" value="CAZ80491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836300.1">
        <property type="nucleotide sequence ID" value="XM_002836254.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7J8"/>
    <dbReference type="STRING" id="656061.D5G7J8"/>
    <dbReference type="GeneID" id="9186970"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002606001"/>
    <dbReference type="HOGENOM" id="CLU_792714_0_0_1"/>
    <dbReference type="InParanoid" id="D5G7J8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="130"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="284"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="141"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ80491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="350" mass="39453" checksum="A9697DD0EBBF86F1" modified="2010-06-15" version="1">MSKPNITRHFRGGMEELKTADGITLYAYKSVLGQGTSPYLNTPWECFTTSTVERFLEYCYQGDYSPPKPAKLPLATPTSSVVDADGNAIGVFQTAAGHLPCCQLANRRAAPQGDHHLCCSCIAIQEDDTEEDEGSKYDGVDGSDTEYADDKDDSGPSRHPHGLDYFEVFFTHAELYILSQTQGQSLLAALCLNRLRTALEKAAKAPVRPRFPTNLSRLLSYAYEHNNVRLIDDNQAHDLQNLASSCAAMHIKDMKEECSSLMGRGGKMAEDLMKEVADRAISLDSVLEMEMEKMETEKMEMKEKMKKMETEWMEVEKKLEEVETEKTEMEKMLEKVETEKAEMKKKLKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7P6</accession>
    <name>D5G7P6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="12">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00004670001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA2/NAM7 helicase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430031">
        <property type="protein sequence ID" value="CAZ80539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836348.1">
        <property type="nucleotide sequence ID" value="XM_002836302.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5G7P6">
        <property type="interactions" value="268"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G7P6"/>
    <dbReference type="GeneID" id="9187018"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004670001"/>
    <dbReference type="eggNOG" id="KOG1801">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000459_2_0_1"/>
    <dbReference type="InParanoid" id="D5G7P6"/>
    <dbReference type="OMA" id="GCIKCIM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016604">
        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001147">
        <property type="term" value="F:transcription termination site sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18042">
        <property type="entry name" value="DEXXQc_SETX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000326">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001152">
        <property type="entry name" value="tRNA-splicing endonuclease, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045055">
        <property type="entry name" value="DNA2/NAM7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024481">
        <property type="entry name" value="Helicase_Sen1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056474">
        <property type="entry name" value="SEN1_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887:SF495">
        <property type="entry name" value="HELICASE SENATAXIN ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23576">
        <property type="entry name" value="SEN1_barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12726">
        <property type="entry name" value="SEN1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="11">
        <location>
            <begin position="1820"/>
            <end position="1835"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="948"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1755"/>
            <end position="1819"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1831"/>
            <end position="1971"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="1368"/>
            <end position="1447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="1761"/>
            <end position="1777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1861"/>
            <end position="1875"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1876"/>
            <end position="1885"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1890"/>
            <end position="1907"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1908"/>
            <end position="1923"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="1952"/>
            <end position="1965"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ80539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1971" mass="219831" checksum="F69F9349473D6999" modified="2010-06-15" version="1">MSAPDALDVEDIESGLRLVHSLPEQVHWLCDKIEKRDDGVEVKYEGDKSRRNRSVTHAMVLLSFADQEATQAWFTAALNAQLSRCPRCVEQFYILKKGLYRELLEADNKDNVDMFFNFLHTCDANRVGPVLKEVASELRNSPTVESWHQTFQSRAQYRNGVYEVFCCPDLLRDPEINADFTDICATRKVFPEGPGEGLISFLFRDDERLRFPVEHSWKELGPHITPEVFETHLLATLEAQVQRMEVQSDPGQLQLFFAGLVIIVQSVSKELIMKCICGAQNDPIKLAVNRISNNASYLPALLGLFDALMIQLGFEFWDVAQPLTPSALIDTLLANGIFANLLKRAPQDTSGERLLIGLTGWMSSLVASVRPLSRATPAAPLLRLLLKDRSLPALSKGICLKEGMKILSMTLSGVSAHPIEGEGGRMILRQTNELFDEYTLLVVEIAISFSTRFNDQIMANHMGIAQKNAYNAMLLALELDVKVMKTDFSLLSKKKPEIPHAETSIRTSLWDAVCKNIPINHVKLATDLLLIFCEIFGIGKSVHPKGIDTGSEADRRRDAFNKALDILSRCFARIFQRISQFAPDKLKMVVNGEHSRIALFVGVIFPPKDVAFGAEDILLSCYGVDSRENAIHAMFDENLAMALSAVTAISRTQAKSSVFDLMPAMIRFNQEILEVLCAPDKGVISNIDLENWTKTALRVYWDIQWRVVDYATELFRRFWTFEQAFRAKGPDNESTRPQREASWRARLLQDPSKALDSIAALLTIQDMHLLSSSQELMCNILELLAKENVDVEDEFVNNMRPILFSDSRAGARTMTNLVEAQKAQLMHALSAVRPDFAPLEAKSKIVEISDDEYDDIPDDDLIRASERSLRIASRKQARLDFPVAPTPPTYEQKLHKVGVSKSSSIIDDLRVGTPKAISVVPRNEFLLKRKADLAASEAAKAARLELKKKEQAPVVPSSDEDDSSDDGGGNGLFRLGNSVQRSTKPIPEPKLIDAKPKNLLRQPAVPTRRVKDNRARVAPDLSPLYKQVLKWEFFHDDVFPPGLSATNYSKVASTFGSYAAYKKTFEPLLLLEAWQALKQAKEEAPQPVVKLNLVTRMSADNFVELEITIDGMNERNRWLESDVVLVSVGKKPLASKDSPHCLARVHSLKKKFPGANTTEVQLRCDPSPQMVQENMRNGSTLYATRIMGFVPTEREYSALMCLKYYDLEQEILAAKPSSLEEPTEKQIVRTRGLYKVNEPQARAILSAVKNTGFTLIQGPPGTGKTKTVVGIVGALLTPKVGSTVIQIPGSMNKSPKPTTKKLLVCAPSNAAVDELVLRFKKGILTAKGEEMQPKVVRIGKSDAVNFTVRDVTLDELVERKMAPTKESANSKNADMDELRQKHRAILDERDAKLKQLEDARAKSIDPGTLQSEIDSLNATLRETRRSLDLKRDQKKESSRNAEVLKRRIQQEIMDEAHIICATLSGTGHDLLRNINVDFETVIIDEAAQSVELSALIPLKFGCEKCILVGDPKQLPPTVLSREAAKFSYEKSLFVRMQENHPKDVHLLSIQYRMHPMISSFPRKQFYDSELEDGENMKELRTEVWHKNPIYAPYRFFNIAGQESAGGLHSLVNRQEAQSALSLYQRLTADFPQTNFDGKIGIITPYKQQINLLKTTFRDVYGENICDTIDFNTTDAFQGRERDIIIFSCVRASQEGGIGFLSDVRRMNVGLTRAKFSLFVLGHSTSLMRNRLWASLVQDAKDRGVFDEETFAEFGSNAKGNRAGGTGAFRGSTRGRGGQARNSTRNTPPPVAPKVDPDAMDIDEPEPQAPHQPNPSHIRLCFECNQPGHIANSCPNRQGPRNHPSRNSNGPGPRPMPPAGRGNDHTQNQHHERLPQEHNQLPNQPTRAPKRPIDGEGEVPSKRQHLENRPSSANQVTPGPSTVPVTIRPPHKKKAQRRSEPPAVFINPKRGGGRGGRGGGGRGGGQPSRPKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7Y6</accession>
    <name>D5G7Y6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00002693001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430034">
        <property type="protein sequence ID" value="CAZ80629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836438.1">
        <property type="nucleotide sequence ID" value="XM_002836392.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7Y6"/>
    <dbReference type="GeneID" id="9181892"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002693001"/>
    <dbReference type="eggNOG" id="ENOG502S2K4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026112_0_0_1"/>
    <dbReference type="InParanoid" id="D5G7Y6"/>
    <dbReference type="OMA" id="ERMNVTP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16616">
        <property type="entry name" value="mRING-HC-C4C4_Asi1p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22696">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22696:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072237" evidence="3">
        <location>
            <begin position="20"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="231"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="415"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="460"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="470"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="482"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="517"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ80629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="663" mass="71993" checksum="9CCBD7CB22BEABFE" modified="2010-06-15" version="1">MRLTPIVLCLFHIYSISLALRCQTSEVYLSDGSPRPAGGFLWQVARVGNWWMDDLQVCEAVGMIPDEAWAEQPIKGSLALLWPFYKTVCFSQFVESFICAVTARNPAAETGMTLMEHSLAFAEAEFLAVPKVFEIQSAPPDGETTVADNRRVLVHTEKNVPPEVLYISLISALGHLTSHILGVFNRQAKHRLLSTGIWGVAFLSGFAWALIFRSESGAVLNFPTVCVVGFIPHLVIFTGIFVCGAIYALALVLTSLLPPPSVSERPSFRAGLDNLQANLTLGTVTINNSDDFYTTLLKLGFQCLTAASEATYLNEGRVVRLPAWTWLEDQRTKVLDSQRKLGIGGGGAAPGLVGTGVGKGPYSQERKDVKGVMAPKKGRSGTGSSRWAGAGEMMRGVSVLLVSLTSRWVPTLFRTRERDPPDTPESVDANQGPILEVEEEDANSTSLYTRFIGNIEGPLWADSDASGDFQPDDSIDDDDDASTVSSAASDTAPLTDFPYDSDDDGFQRSGTRTPTQSHPYPFHDSSSSPSPPRSPFTEIFSTAADLATLLDPQTPEDRAQAQALGRHLKAPNILTRSTYHSANGASTSLESIILQQRRQSPATPASSYSSCVVCQSATRTIIVWPCRCLALCEDCRVCLAHNNYKSCVCCRQGVEGFSRIYVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="105" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8D9</accession>
    <name>D5G8D9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Pentafunctional AROM polypeptide</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="23">3-dehydroquinate synthase</fullName>
                <shortName evidence="23">DHQS</shortName>
                <ecNumber evidence="23">4.2.3.4</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="23">3-phosphoshikimate 1-carboxyvinyltransferase</fullName>
                <ecNumber evidence="23">2.5.1.19</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="23">5-enolpyruvylshikimate-3-phosphate synthase</fullName>
                <shortName evidence="23">EPSP synthase</shortName>
                <shortName evidence="23">EPSPS</shortName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="23">Shikimate kinase</fullName>
                <shortName evidence="23">SK</shortName>
                <ecNumber evidence="23">2.7.1.71</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="23">3-dehydroquinate dehydratase</fullName>
                <shortName evidence="23">3-dehydroquinase</shortName>
                <ecNumber evidence="23">4.2.1.10</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="23">Shikimate dehydrogenase</fullName>
                <ecNumber evidence="23">1.1.1.25</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="30" type="ORF">GSTUM_00004770001</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="30 31" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="30 31">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="22 23 24">The AROM polypeptide catalyzes 5 consecutive enzymatic reactions in prechorismate polyaromatic amino acid biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23 24">
            <text>3-dehydroquinate = 3-dehydroshikimate + H2O</text>
            <dbReference type="Rhea" id="RHEA:21096"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16630"/>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
            <dbReference type="EC" id="4.2.1.10"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>3-phosphoshikimate + phosphoenolpyruvate = 5-O-(1-carboxyvinyl)-3-phosphoshikimate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21256"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57701"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
            <dbReference type="EC" id="2.5.1.19"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:21257"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23 24">
            <text>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate = 3-dehydroquinate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21968"/>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
            <dbReference type="EC" id="4.2.3.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21 23 24">
            <text>shikimate + ATP = 3-phosphoshikimate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13121"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.71"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23 24">
            <text>shikimate + NADP(+) = 3-dehydroshikimate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17737"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16630"/>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="23 24">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="23 24">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="23 24">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 2/7.</text>
    </comment>
    <comment type="pathway">
        <text evidence="23 24">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7.</text>
    </comment>
    <comment type="pathway">
        <text evidence="23 24">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 23 24">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 23 24">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="23 24">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 23 24">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the EPSP synthase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="23 24">In the 2nd section; belongs to the EPSP synthase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the 2nd section; belongs to the type-I 3-dehydroquinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="23 24">In the 3rd section; belongs to the shikimate kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="23 24">In the 4th section; belongs to the type-I 3-dehydroquinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="23 24">In the C-terminal section; belongs to the shikimate dehydrogenase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="24">In the N-terminal section; belongs to the dehydroquinate synthase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the N-terminal section; belongs to the shikimate kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="23">In the N-terminal section; belongs to the sugar phosphate cyclases superfamily. Dehydroquinate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.4" evidence="23"/>
    <dbReference type="EC" id="2.5.1.19" evidence="23"/>
    <dbReference type="EC" id="2.7.1.71" evidence="23"/>
    <dbReference type="EC" id="4.2.1.10" evidence="23"/>
    <dbReference type="EC" id="1.1.1.25" evidence="23"/>
    <dbReference type="EMBL" id="FN430042">
        <property type="protein sequence ID" value="CAZ80782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836591.1">
        <property type="nucleotide sequence ID" value="XM_002836545.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5G8D9">
        <property type="interactions" value="435"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G8D9"/>
    <dbReference type="GeneID" id="9186002"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004770001"/>
    <dbReference type="eggNOG" id="KOG0692">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001201_1_2_1"/>
    <dbReference type="InParanoid" id="D5G8D9"/>
    <dbReference type="OMA" id="SWANMSW"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00085"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003855">
        <property type="term" value="F:3-dehydroquinate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003856">
        <property type="term" value="F:3-dehydroquinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003866">
        <property type="term" value="F:3-phosphoshikimate 1-carboxyvinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004764">
        <property type="term" value="F:shikimate 3-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004765">
        <property type="term" value="F:shikimate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00502">
        <property type="entry name" value="DHQase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08195">
        <property type="entry name" value="DHQS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01556">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01065">
        <property type="entry name" value="NAD_bind_Shikimate_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00464">
        <property type="entry name" value="SK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1090.10:FF:000007">
        <property type="entry name" value="Pentafunctional AROM polypeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000135">
        <property type="entry name" value="Pentafunctional AROM polypeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1970:FF:000007">
        <property type="entry name" value="Pentafunctional AROM polypeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001256">
        <property type="entry name" value="Pentafunctional AROM polypeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.65.10.10:FF:000007">
        <property type="entry name" value="Pentafunctional AROM polypeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.65.10.10:FF:000008">
        <property type="entry name" value="Pentafunctional AROM polypeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1090.10">
        <property type="entry name" value="Dehydroquinate synthase-like - alpha domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.10">
        <property type="entry name" value="Enolpyruvate transferase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00210">
        <property type="entry name" value="EPSP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03143">
        <property type="entry name" value="Pentafunct_AroM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00109">
        <property type="entry name" value="Shikimate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016037">
        <property type="entry name" value="DHQ_synth_AroB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030960">
        <property type="entry name" value="DHQS/DOIS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056179">
        <property type="entry name" value="DHQS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001381">
        <property type="entry name" value="DHquinase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001986">
        <property type="entry name" value="Enolpyruvate_Tfrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036968">
        <property type="entry name" value="Enolpyruvate_Tfrase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006264">
        <property type="entry name" value="EPSP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023193">
        <property type="entry name" value="EPSP_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008289">
        <property type="entry name" value="Pentafunct_AroM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031322">
        <property type="entry name" value="Shikimate/glucono_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013708">
        <property type="entry name" value="Shikimate_DH-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010110">
        <property type="entry name" value="Shikimate_DH_AroM-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000623">
        <property type="entry name" value="Shikimate_kinase/TSH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023000">
        <property type="entry name" value="Shikimate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006151">
        <property type="entry name" value="Shikm_DH/Glu-tRNA_Rdtase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01356">
        <property type="entry name" value="aroA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01357">
        <property type="entry name" value="aroB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01093">
        <property type="entry name" value="aroD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01809">
        <property type="entry name" value="Shik-DH-AROM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21090">
        <property type="entry name" value="AROM/DEHYDROQUINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21090:SF5">
        <property type="entry name" value="PENTAFUNCTIONAL AROM POLYPEPTIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01761">
        <property type="entry name" value="DHQ_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24621">
        <property type="entry name" value="DHQS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01487">
        <property type="entry name" value="DHquinase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00275">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01488">
        <property type="entry name" value="Shikimate_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08501">
        <property type="entry name" value="Shikimate_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01202">
        <property type="entry name" value="SKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000514">
        <property type="entry name" value="Pentafunct_AroM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01100">
        <property type="entry name" value="SHIKIMTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56796">
        <property type="entry name" value="Dehydroquinate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="EPT/RTPC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00104">
        <property type="entry name" value="EPSP_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00885">
        <property type="entry name" value="EPSP_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01128">
        <property type="entry name" value="SHIKIMATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 23 24" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="17 23 24" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="14 23" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 23 24" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 23 24" id="KW-0418">Kinase</keyword>
    <keyword evidence="18 23 24" id="KW-0456">Lyase</keyword>
    <keyword evidence="10 23 24" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19 23" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="15 23" id="KW-0521">NADP</keyword>
    <keyword evidence="11 23" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16 23 24" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 23 24" id="KW-0808">Transferase</keyword>
    <keyword evidence="13 23 24" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="3-dehydroquinate synthase N-terminal" evidence="27">
        <location>
            <begin position="79"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="3-dehydroquinate synthase C-terminal" evidence="29">
        <location>
            <begin position="193"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="Enolpyruvate transferase" evidence="25">
        <location>
            <begin position="394"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="domain" description="Shikimate dehydrogenase substrate binding N-terminal" evidence="28">
        <location>
            <begin position="1276"/>
            <end position="1356"/>
        </location>
    </feature>
    <feature type="domain" description="Quinate/shikimate 5-dehydrogenase/glutamyl-tRNA reductase" evidence="26">
        <location>
            <begin position="1393"/>
            <end position="1453"/>
        </location>
    </feature>
    <feature type="region of interest" description="3-dehydroquinate synthase" evidence="23">
        <location>
            <begin position="1"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Shikimate kinase" evidence="23">
        <location>
            <begin position="846"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="region of interest" description="Shikimate dehydrogenase" evidence="23">
        <location>
            <begin position="1271"/>
            <end position="1554"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for 3-dehydroquinate synthase activity" evidence="23">
        <location>
            <position position="246"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for 3-dehydroquinate synthase activity" evidence="23">
        <location>
            <position position="261"/>
        </location>
    </feature>
    <feature type="active site" description="For EPSP synthase activity" evidence="23">
        <location>
            <position position="807"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for 3-dehydroquinate dehydratase activity" evidence="23">
        <location>
            <position position="1162"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate; for 3-dehydroquinate dehydratase activity" evidence="23">
        <location>
            <position position="1190"/>
        </location>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <begin position="48"/>
            <end position="50"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <begin position="85"/>
            <end position="88"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <begin position="116"/>
            <end position="118"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate</name>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <begin position="141"/>
            <end position="142"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate</name>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate</name>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate</name>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <begin position="181"/>
            <end position="184"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <begin position="196"/>
            <end position="199"/>
        </location>
        <ligand>
            <name>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate</name>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <begin position="250"/>
            <end position="254"/>
        </location>
        <ligand>
            <name>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate</name>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
        </ligand>
    </feature>
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        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate</name>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate</name>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="342"/>
        </location>
        <ligand>
            <name>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate</name>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <begin position="853"/>
            <end position="860"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00044633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00054455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRNR" id="PIRNR000514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF00275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF01488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="Pfam" id="PF01761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="Pfam" id="PF08501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="Pfam" id="PF24621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="EMBL" id="CAZ80782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1554" mass="168596" checksum="05C94511E674CB98" modified="2010-06-15" version="1">MSGDNGSTVTTVQILRKDTIKVGYDLTDTITVDLLQNLISSTYVLITDTNLAPLYLPAFESSFNKNALKYSPEARLLSYIIPPGEYSKSRSTKAEVEDWMLSEKCTRDTVVIALGGGVIGDMIGYVAATFMRGVRFIQVPTTLLAMVDSSIGGKMAIDSPHGKNLIGAFWQPERIFIDMKFLETLPEREFVNGMAEVVKTAAIWDEAEFARLEQSSGLILETVRAKSSGNKAQIGNAHVVSADEREGGLRNLLNFGHSIGHAIEAILFPQMLHGECVAIGMVKEAELARHLGVCKPGAVARLSKCLTSYGLPTSLEDKRVKKLTAGKHCGVERMLSIMGVDKKNDGRKKKVVLLAAIGRTYEKKASVVDDDSIRVILSPSVIVHSQRSGVPELEVTPPGSKSISNRALVLAALGSGTCHVKNLLHSDDTEHMLTALHNLGGIQYSWEEDGDVLVVNGNGGKLRACGDELYLGNAGTAARFLTSVATLVSPSGEHDSIVLTGNARMKQRPIGSLVDALRSNGSSVEYLESQGSLPVRVPSTSGLMGGRIELEAKVSSQYVSSVLICAPYAREPVTLSLVGGKPISQLYIDMTIAMMSSFGIDVTKSTAEEYTYHIPQGSYKNPAVYVVESDASSATYPLAIAAITGNTVTVPNIGRKSLQGDARFAVDVLRPMGCTVEQSDYSTTVRGPPKGQLRPLPHVDMEPMTDAFLTASVLAAVAKGVERSSITGIANQRVKECNRIAAMVHELGKFGVTAGELEDGIWIQGIDYNLLKTPEGVHCYDDHRVAMSFSVISLIAPNPVILQERKCVEKTWPGWWDVLSTSFNVHLGGVELLENPQVMEVKKGSNRTIVIIGMRGAGKTTMGIRAAKILNRPVLDLDTVLEQEQKSALPEIIKKHGWDGFRKLELELLQRHLKAHPDSYIFACGGGIVETPVARNLLKQYCSSGGIVIHVHRNIERVMEFLSIDKTRPAYVEDMVGVWNRRKPWYTECSNYQYYSPIVSESDLAIAQADFSRFLAIVTGNSDYHERLLKKTRSFFVSLTFPNLEEVTGAINSVTIGSDAVEVRIDLLIDPTDSNGVATPAFVSEQLAVLRGKTQLPVVFTIRTKSQGGKFPDEDHQRALELYLAGIRMGLEYIDLETTWPVALLEKVTSGKGYSKIIASHHDLGGDLCWRNASWISKFNMTLKYGDVIKLIGQAKSIQDNYDLENFRKYAATHETRMIALNMGEKGKLSRILNTFLTPVTHPALPFKAAPGQLSTVEIHQALSLMGMLETKKFYIFGTPLSHSRSPALHNTLFQSLGLPHTYHLFESADVSATKEIMNTENFGGASVTIPHKISIIPLLSSISAHAQAIGAVNTVIPVTTEDGTRRLTGDNFDWVGIQNSLRIGGATEPEGHSTATALVIGAGGTSRAAVYALHMMGYGRIYVVNRTPENLKSLVEAFPKEYNLMAINSLAEAKSVEVKPITAVCTVPADKPIDDALKVILWELLAGGACAGVLTEMAYKPTVTEVMTLAEGAGWRVVPGLEGLVGQGVAQFEVFTGGIVPEYGAARAAVLGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8I9</accession>
    <name>D5G8I9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00002894001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430046">
        <property type="protein sequence ID" value="CAZ80836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836645.1">
        <property type="nucleotide sequence ID" value="XM_002836599.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8I9"/>
    <dbReference type="STRING" id="656061.D5G8I9"/>
    <dbReference type="GeneID" id="9186056"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002894001"/>
    <dbReference type="eggNOG" id="KOG1804">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001666_7_1_1"/>
    <dbReference type="InParanoid" id="D5G8I9"/>
    <dbReference type="OMA" id="LYYFDEC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035194">
        <property type="term" value="P:regulatory ncRNA-mediated post-transcriptional gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045055">
        <property type="entry name" value="DNA2/NAM7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887:SF322">
        <property type="entry name" value="HELICASE MOV-10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0347">Helicase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="3">
        <location>
            <begin position="391"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase-like C-terminal" evidence="4">
        <location>
            <begin position="682"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="59"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="896"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1036"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="24"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="66"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="92"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1036"/>
            <end position="1045"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ80836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1106" mass="122905" checksum="256E7231DB4D0F84" modified="2010-06-15" version="1">MSPPTPLRIVPPSPLASQAAGYVPNTSHTPLSSTSQALPTTAMSYAMVAKCNNAPTFPSGVFQPPLSRTPQQASLPHNAQQHRQQQGIAMHPPQPFRPNPLQPPFSPSSQLTPGASSSSPKPWQPDVYTLPFIPHYLQAINTLPAVLVASLPAPSIPYHSFISSFCGSTFLRPLPPAPAVPPVPSASVPVPSSDLTPTIYSKYFFNLLSHELSAQHTEFRKYDLFNVPIYVQEPSQSLYRLDVPGIREDNPPVLLGDVVKLRQVRQPGYMGMGGTFTGYQYDACVYGMDKTVGYIVLRVDHLVLESGRFNVCFVVQEKQWTGATRAIEDLGAELRGTGSREVAEGAKGNGVEANNRPGVFVRRMLFPGKEDGIWQTTLSRGVFKRRWFDRELNYEQQKAVDTILQQNYGNVPYLISGPPGTGKTKTIVEVALQLIFTPSNPKHHILLCAPSQEAADTLALRLVPYLNPRVLFRLQSSTRTFPEVPSELLPYSHIANDMFSLPEWKKLMGFRVVVCSTRDAEILVEARCTNKDLARWEKSVFDSLRGIGDESDGNDPTASGVVEKGQIVNVHWTALLLDEAAQGIEPEVAVAVSVVVPPEEASVDRPIFVMAGDQRQLGPRTVSRVPQLDMSLFERLFLRELYSQHPLARQSFTHAYKQSPRVWSAERILERKKALLPYLRPPFTNLIRNYRSHPAILAVPSALFYNDTLVPEASNTNYLEGWSGWKGRRGIPVKFCLNGGMDEWHEEGVSFYNLREIKIACNIAKNLVSSGLIAPHEISIMAQFREQIRRLRRALRSPDYNLRDVNVGPIESYQGSEHRFVIVCTTRSRERFLEGDLERGLGVIFENRRVCVAMTRAKQGLIVIGNPWILGRDPIWRAWMGFCWRHNSVEKDFFEEEEEDKAFDPQANEKDVMLGDEDSQPNRENPPLPNGHSNHTSGKLPAITELKVNNWQPPEDERETPQYISRLETALVYRSRVNNGYTGALGAVHGGFDEDDPMWTAGIVAEEAVRGGIGNRDLLEPTAGTGMPWPHLLDNTHSVDGSLQGLTRRDSSEEEGVDGEGAGADADKQAEPAARIGKNKKGFSEVKATETTQTSLFLAGPGKLWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8J0</accession>
    <name>D5G8J0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00002895001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430046">
        <property type="protein sequence ID" value="CAZ80837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836646.1">
        <property type="nucleotide sequence ID" value="XM_002836600.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8J0"/>
    <dbReference type="STRING" id="656061.D5G8J0"/>
    <dbReference type="GeneID" id="9186057"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002895001"/>
    <dbReference type="eggNOG" id="ENOG502RDJ2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008050_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8J0"/>
    <dbReference type="OMA" id="NDGWREL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd08681">
        <property type="entry name" value="C2_fungal_Inn1p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037791">
        <property type="entry name" value="C2_fungal_Inn1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052981">
        <property type="entry name" value="Ingression_C2_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47052">
        <property type="entry name" value="CONSERVED SERINE PROLINE-RICH PROTEIN (AFU_ORTHOLOGUE AFUA_2G01790)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47052:SF3">
        <property type="entry name" value="INGRESSION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="2">
        <location>
            <begin position="6"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="145"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="726"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="210"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="335"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="365"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="400"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="431"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="828"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="844"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="879"/>
            <end position="892"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ80837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="963" mass="107519" checksum="48ADD8728E80919E" modified="2010-06-15" version="1">MPRAGIKEAHPAGIWSDLSVDGPEIGELVVILDKAKNLPNRKKIGKQDPYVVVRLGKNAQRTDADVRGGQTPRWDEELRFKIRESPDYKTLKITVFNDDKKADLIGETTLKIDKILVTGGGINDGWRELRCKGRYAGEILVELTYYDGPDSDDPDNVEPPAEKKRTRRDLGGKEREREKEAARKVGGAREMVSSRDKSVKRRPLPPDPTRSSKEDVSRPVPVPEKRTRQSRHSYRSGYEDNQPHNQHSYPEPQHPHHRNPRGSSMAMAPDHLPVATGAYDPYGDDHIPGGDMFGGFEDDDPHGQPPPPPPPIHRRHYDASPVHHSPNPPYPHQQTVHKRHKSQHQLKHHQSVPDWQHHQQQSHSPDPREREMHRSNSYDPRLSAQDQYGGSYPGPRTRYHGQDDYDEYVHSPNAVASTAHLRRERPLTYHGESSGPPYGPSPASSFEDSRDEDDAPPPPPPPHGHSPASARFDEPNWGNHTPKTKEEEDLGLPSYELSTQLAHRRSHSNMNARALEVQMPAVGVETPPTRNGMPIPPSLVPGIDPLVAEQMSGRIEVERRLSFVDTSKGPLQIEGPPLSHSQAMQHRSSQNYHRPQVEEVQDVQLYQPQLLPEDQVVGRPVTVRRGREPRTAPMVKPMPIHGERDDVVPVVGATTSSVRRNSTVAMVIPERKPVPPAEPVFDGLPFSPDSFDLINPGPSASLEAAINGPGPTYNTPQQAEETARRWNAESKRGKLPSKMIVPGTNRVLDGTDVLPPESFAPEPERRGGRSPRPPPPVPAEYRRHSTGRQRSPSPQPPKRGERLALIGPVPPPLPKKVSIRTPSPQPGRSEKERERAKLQKRLRNSQSMAALPSNSRKHASSRDRHSIGPGAMVMYNPGSERERQMERDRDSRALVPAKGNDRYGYNSDQPRGGRYKQHLPAPPLPAKIPINTAGMSREDYLLSQEMSLISIGPTSGGRTRRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8J2</accession>
    <name>D5G8J2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00002898001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430046">
        <property type="protein sequence ID" value="CAZ80839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836648.1">
        <property type="nucleotide sequence ID" value="XM_002836602.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8J2"/>
    <dbReference type="STRING" id="656061.D5G8J2"/>
    <dbReference type="GeneID" id="9186059"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002898001"/>
    <dbReference type="eggNOG" id="ENOG502QQNB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033094_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8J2"/>
    <dbReference type="OMA" id="FYLFHNY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03507">
        <property type="entry name" value="Delta12-FADS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012171">
        <property type="entry name" value="Fatty_acid_desaturase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32100">
        <property type="entry name" value="OMEGA-6 FATTY ACID DESATURASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="317"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid desaturase" evidence="3">
        <location>
            <begin position="124"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ80839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="489" mass="54745" checksum="39397ECA4707D472" modified="2010-06-15" version="1">MATRSRTRKSSISQASTPLSANSTSSSKAPSASSSSTSLSSMAPVNEKSRKPLLDTYGNEFEFPDFTIKQIRDAIPAHCYKSSTLRSLGYVARDLALVSSNFYAFKFHIIPALAEYHPVARFAAWGVYSFIQGLFGTGLWVLAHECGHGAFSPSKTVNDLVGWVLHSSLLVPFWSWKISHGKHHKATGHLERDMVFVPKSREEYSRRYAGGIGHDIVELTEETPIATLVHLIGQQLLGWPFYLINNVTGHPYHERQSEGRGVGKRNGWFGGVNHFDPRSPLYENRDAKWILLSDVGIAVTMYFLYQAVQIWGLANIAIWYFGPYLWVNHWLVAITFLQHTDPSLPHYHPESWTYTRGAAATIDREFGFIGRHLLHGIIETHVAHHFVSSIPFYNADEATEAIKKVMGKHYRSDTEGGPAGFLRALWRSSRWCQFVEPSEGAEGEGKGVVFFRNRNGLGVPPVSQEKIVSRVPFEKKGPVVQVAEVSDSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8J4</accession>
    <name>D5G8J4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="12">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SEY1</name>
        <name evidence="12" type="ORF">GSTUM_00002904001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endoplasmic reticulum membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <text evidence="8">Enriched in the cortical ER. Concentrated in punctae along the ER tubules.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the TRAFAC class dynamin-like GTPase superfamily. GB1/RHD3 GTPase family. RHD3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430046">
        <property type="protein sequence ID" value="CAZ80841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836650.1">
        <property type="nucleotide sequence ID" value="XM_002836604.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8J4"/>
    <dbReference type="FunCoup" id="D5G8J4">
        <property type="interactions" value="57"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G8J4"/>
    <dbReference type="GeneID" id="9186061"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002904001"/>
    <dbReference type="eggNOG" id="KOG2203">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011270_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8J4"/>
    <dbReference type="OMA" id="PIIKMTE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048309">
        <property type="term" value="P:endoplasmic reticulum inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016320">
        <property type="term" value="P:endoplasmic reticulum membrane fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01851">
        <property type="entry name" value="GBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000727">
        <property type="entry name" value="Protein SEY1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03109">
        <property type="entry name" value="Sey1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030386">
        <property type="entry name" value="G_GB1_RHD3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008803">
        <property type="entry name" value="RHD3/Sey1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046758">
        <property type="entry name" value="Sey1/RHD3-like_3HB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45923">
        <property type="entry name" value="PROTEIN SEY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45923:SF2">
        <property type="entry name" value="PROTEIN SEY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05879">
        <property type="entry name" value="RHD3_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20428">
        <property type="entry name" value="Sey1_3HB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51715">
        <property type="entry name" value="G_GB1_RHD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 8 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="topological domain" description="Cytoplasmic" evidence="8">
        <location>
            <begin position="1"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="737"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="topological domain" description="Lumenal" evidence="8">
        <location>
            <begin position="756"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="762"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="8">
        <location>
            <begin position="780"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="domain" description="GB1/RHD3-type G" evidence="11">
        <location>
            <begin position="59"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="490"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="69"/>
            <end position="76"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ80841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="852" mass="95296" checksum="F45496EFC1B75A99" modified="2010-06-15" version="1">MSVDAGGGHNRSGSMGRNGTRARDDSNSYSSGIQVIDENKEFTPYLPAYLTAQNLITAGFSYHVVAVFGSQSTGKSTLLNHLFGTRFSVMDEQARRQTTKGIWMSRAIEDTKKSDPREMGNNILVMDVEGTDGRERGEDQDFERKSALFALATSEVLIVNIWEHQVGLYQGANMGLLKTVFEVNLGLFLKDRSTTHRSLLFFVIRDHVGHTPLQNLSNTLMQDLYRIWSSLSKPPGLEDSKISDFFDFEFVALPHKILQPERFVEETQKLRKRFREGISPQVEAMSGSNGVERRGDGGGVFLPAYHRRIPADGFPHYASGVWTQIVINKDLDLPSQQELLAQYRCDEIAATCTAGFNEIMSPFEEQAKTGRVLEGLGSAMKEALGEAMGGFEESGGRYHKGVFERKREDLRENLEGRLRGLVVGQFSALSKRAVQEFTEEITGILKLANASGQSTSSNYDFAAVVNQTRSKVVDRFITEASESYIPGAEWSNYENELASLQAALDEIAARLRGEEMKRLVARLEKAIRSKLAEPVELEFRKMEDSSKEKGNLWDRVWKVWTEAVGEGVEGFLARAGGLNATEKERDIGAWKLRKKAWGVLKAKIEEEVMEGNILLKLRENFEDRFRYDEQGVPRVWKPTDPIEQAYTTAREATLGLIPLISRIRLASGVEPPLTEFLSDPPSNFEEDVDDMGVAKDEFVVLGEAKQVDLSTRFKRMADAVFVEAKRSTVSSVAQIPIYFWGLMLALGWNEIWAVLRSPVYFIFLLMCCAAAYVVHTLNLWGPILRVTNAMSQQAVDIGKERLRDLVADPNVVYPAGLNGTRGVDDGSGAISLQTLDGEGRKRARIKREDDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8T8</accession>
    <name>D5G8T8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="14">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00004833001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Forms homooligomers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SHO1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430053">
        <property type="protein sequence ID" value="CAZ80931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836740.1">
        <property type="nucleotide sequence ID" value="XM_002836694.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8T8"/>
    <dbReference type="SMR" id="D5G8T8"/>
    <dbReference type="FunCoup" id="D5G8T8">
        <property type="interactions" value="120"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G8T8"/>
    <dbReference type="GeneID" id="9186440"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004833001"/>
    <dbReference type="eggNOG" id="ENOG502QW7A">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043316_1_0_1"/>
    <dbReference type="InParanoid" id="D5G8T8"/>
    <dbReference type="OMA" id="NIVWIFY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007232">
        <property type="term" value="P:osmosensory signaling pathway via Sho1 osmosensor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11855">
        <property type="entry name" value="SH3_Sho1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000213">
        <property type="entry name" value="High osmolarity signaling protein SHO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035522">
        <property type="entry name" value="Sho1_SH3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735">
        <property type="entry name" value="FCH AND DOUBLE SH3 DOMAINS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735:SF20">
        <property type="entry name" value="HIGH OSMOLARITY SIGNALING PROTEIN SHO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="8" id="KW-0346">Stress response</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="92"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="127"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="13">
        <location>
            <begin position="259"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="165"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="185"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
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            <begin position="223"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="235"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ80931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="318" mass="34399" checksum="4C2E7585B73B2064" modified="2010-06-15" version="1">MQGQDPSMSRGRPYGTSSSRLDFGNIIGDPFALSTISIAIIGWLVGFVSQAIADADKAIPNFPKFAWWSIVYVFFCIIGVIIVILMDSVHKYQLALVGYLSAGMVFTCSAANTLVYTDRGTMQAAGAGHIILSIVQIIWIFYFGSTPDASSHAYVDSFALHKDQKRSQSTRPLSPAYHGGDRPHTSTSSHHAPQMYTSAQLNGFETSSPVPGQAGPDHRQSANRHASPSVSTLTPGPSATPQPQSQGGPPDSEVSAPTEYPYRAKAIYSYDANPEDANEISFAKHEILEVSDVSGRWWQARKQNGETGIAPSNYLILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8Z1</accession>
    <name>D5G8Z1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00004903001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430053">
        <property type="protein sequence ID" value="CAZ80984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836793.1">
        <property type="nucleotide sequence ID" value="XM_002836747.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8Z1"/>
    <dbReference type="GeneID" id="9181267"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004903001"/>
    <dbReference type="eggNOG" id="KOG1289">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004495_2_1_1"/>
    <dbReference type="InParanoid" id="D5G8Z1"/>
    <dbReference type="OMA" id="GIMLQML"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649">
        <property type="entry name" value="AMINO-ACID PERMEASE BAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649:SF15">
        <property type="entry name" value="CHOLINE TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="75"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="329"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="381"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="405"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="447"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="476"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ80984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="528" mass="56964" checksum="3C309B275F07D272" modified="2010-06-15" version="1">MAPDAKELALMSKDMELSVEDKDALKLEAALGHKQVLSRGFGLFSLTSLGIIIANAWSLTGGTIVVAIGNGGPMAVLYGLILVTIFYTLISASLAELVSSIPSSGGVYHWASVTAGPRYGRVVGWYAGFLNLCGWLLSAASISSTLGTELVALYQLSYPEVEWKSWQVFIAYQIVNWMCCCIVIFANRHLPLINKIAFYLSMGGLFTTIMVLAIMSRGRRASDKFVWREYTDLSGWNNDGLCFILGLINAAFAVGTPDCQSHVAEEIPEPEKNVPKGIMIQIGTSFITAFAYLIALFYSINDIELVFDSQSQFPTSEIYRQATGSTPGALGLTAVLFLATFPTLIGTLITGGRTFWTLSRDNAAPFGDYFTRISPTFGSPLRATIAVSVVTTCLGAIQVGSTTAFSALIGSYIVLSTLSYAGAILPHVLTGRKTIVPGPFHLGRAGFAINIISLLYIAVTVVFFCFPFAMPVEAGNMNYTSVIVSGFVVLITFWWFVHGTGNYEGPKYYKEAAKQLADSGSIGNSELQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9A6</accession>
    <name>D5G9A6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00003226001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430057">
        <property type="protein sequence ID" value="CAZ81099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836908.1">
        <property type="nucleotide sequence ID" value="XM_002836862.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9A6"/>
    <dbReference type="GeneID" id="9182850"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003226001"/>
    <dbReference type="HOGENOM" id="CLU_2623786_0_0_1"/>
    <dbReference type="InParanoid" id="D5G9A6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ81099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="78" mass="8648" checksum="A31B2A449BFF8E47" modified="2010-06-15" version="1">MGSKDCLRPGGEPCISDICITCEHLVSRERLRVQHEGLPIAGRLLGSLVYEAFCTESHNCRPSHRLFDSNIPLSVVNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9D6</accession>
    <name>D5G9D6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00003259001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430059">
        <property type="protein sequence ID" value="CAZ81129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836938.1">
        <property type="nucleotide sequence ID" value="XM_002836892.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9D6"/>
    <dbReference type="GeneID" id="9182880"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003259001"/>
    <dbReference type="HOGENOM" id="CLU_2706592_0_0_1"/>
    <dbReference type="InParanoid" id="D5G9D6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ81129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="73" mass="7750" checksum="AA8FECA0FB4D0A66" modified="2010-06-15" version="1">MCGCIICVSMDGNCTVLVQARCGCKAKITLIQSITASDLSKNGMGSPFLIPFPTMVAEHGSIVRYSALCRCAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9H5</accession>
    <name>D5G9H5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA-directed RNA polymerase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00003403001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 12">Belongs to the archaeal rpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430061">
        <property type="protein sequence ID" value="CAZ81168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836977.1">
        <property type="nucleotide sequence ID" value="XM_002836931.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9H5"/>
    <dbReference type="FunCoup" id="D5G9H5">
        <property type="interactions" value="456"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G9H5"/>
    <dbReference type="GeneID" id="9182919"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003403001"/>
    <dbReference type="eggNOG" id="KOG2906">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093932_3_0_1"/>
    <dbReference type="InParanoid" id="D5G9H5"/>
    <dbReference type="OMA" id="MEFCDEC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006386">
        <property type="term" value="P:termination of RNA polymerase III transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd10509">
        <property type="entry name" value="Zn-ribbon_RPC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.10:FF:000005">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012164">
        <property type="entry name" value="Rpa12/Rpb9/Rpc10/TFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001529">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034014">
        <property type="entry name" value="Zn_ribbon_RPC11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001222">
        <property type="entry name" value="Znf_TFIIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239:SF12">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02150">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01096">
        <property type="entry name" value="Zn_ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005586">
        <property type="entry name" value="RNApol_RpoM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00661">
        <property type="entry name" value="RPOL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00440">
        <property type="entry name" value="ZnF_C2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51133">
        <property type="entry name" value="ZF_TFIIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8 12" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="3 9 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIS-type" evidence="13">
        <location>
            <begin position="67"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="10">
        <location>
            <begin position="5"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="5"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR005586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR005586-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR005586-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ81168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="111" mass="12784" checksum="73632373CC262BE5" modified="2010-06-15" version="1">MLLFCPQCSNSLTVSRAPETGTNRLECRTCPYQFLLDQAYYERKSMKRKEVEDVLGGEGAWDNVDQTDAQCPVDECGGTRAYFYMVQIRSADEPMTTFYKCVSCAHKWREN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9W2</accession>
    <name>D5G9W2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00005099001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL20 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430064">
        <property type="protein sequence ID" value="CAZ81305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837114.1">
        <property type="nucleotide sequence ID" value="XM_002837068.1"/>
    </dbReference>
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        <property type="interactions" value="1159"/>
    </dbReference>
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    <dbReference type="GeneID" id="9188548"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005099001"/>
    <dbReference type="eggNOG" id="KOG0829">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080773_1_1_1"/>
    <dbReference type="InParanoid" id="D5G9W2"/>
    <dbReference type="OMA" id="CIFAKND"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.10:FF:000001">
        <property type="entry name" value="60S ribosomal protein L18a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023573">
        <property type="entry name" value="Ribosomal_eL20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021138">
        <property type="entry name" value="Ribosomal_eL20_eukaryotes"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10052">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L18A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01775">
        <property type="entry name" value="Ribosomal_L18A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002190">
        <property type="entry name" value="Ribosomal_L18a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160374">
        <property type="entry name" value="RplX-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein eL20" evidence="5">
        <location>
            <begin position="53"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR002190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ81305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="148" mass="17469" checksum="5602B550DEF8C9BB" modified="2010-06-15" version="1">MRLFAPNTVVAKSRFWYFLKKLRKVKKSAGEIVSLNVIHEKRPQKVKNFGIWIRYDSRSGTHNMYKEYRELSRTDAVQSLYQDMAARHRARFRSIHILKVVEIEKASDVKRPYLRQLITKGLKFPLPHRVPKSVSAKVFSAKRPSTFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9X9</accession>
    <name>D5G9X9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00003433001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430065">
        <property type="protein sequence ID" value="CAZ81322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837131.1">
        <property type="nucleotide sequence ID" value="XM_002837085.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9X9"/>
    <dbReference type="STRING" id="656061.D5G9X9"/>
    <dbReference type="GeneID" id="9188563"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003433001"/>
    <dbReference type="eggNOG" id="KOG1178">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002220_2_1_1"/>
    <dbReference type="InParanoid" id="D5G9X9"/>
    <dbReference type="OMA" id="WHYLIPR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051414">
        <property type="entry name" value="Adenylate-forming_Reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013120">
        <property type="entry name" value="FAR_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43439:SF2">
        <property type="entry name" value="ENZYME, PUTATIVE (JCVI)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43439">
        <property type="entry name" value="PHENYLACETATE-COENZYME A LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23562">
        <property type="entry name" value="AMP-binding_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07993">
        <property type="entry name" value="NAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carrier" evidence="3">
        <location>
            <begin position="571"/>
            <end position="653"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ81322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1061" mass="116153" checksum="2557715170131D2E" modified="2010-06-15" version="1">MVSSPLFRRPRFSLLERLPTDQDDNIRTYPELLAFNAKYNPSHIFCLQGVKDHNAPPVPITFAQAQLSVERCMGWLSKSVEGIHSPREVDGKVGKAAAVGILMGSDVGVIIYMMALLSLGVPVVLLSARLTPVAIAHLLKSTFAGSVIVSQRCQRAVLEAVASYGSAEKVPDIYEPISYDRFLEDEGWGSLEIPPAYDIVEEMDRNVLILHSSGTTGLPKPIYHPHKYMLGYAACHLFEENDRVDGVNCSTLPLFHGFGLLSPSLALSVGKPFCIPPASIIPTATSTLSLLRLSNARSLMSVPSILEDLLYLPQSEGISALRALDFVVVGGGPIKSSVGAALVEAGVKLLNHMGATEIGAIAPIFLPGPDYDWRYLLLRKDIGLRLEDPGDGGGLFKLIGRPFGWSEEFPVQDLLQCNPHAPDSQFKILGRADDLIVLATGEKVLPRMMEMTVANDCKVKEAIAFGDGRFNIGLIVEASIDLDMKDKEQVSAYVEEIWPSVQAGNEFTDNHGKITSKGMIVVTTPSYKQLARTDKGSLTRKTIVQDFAEEIDAAYEKAEMEGIEAMPGSVEDTKAWLKTLVGDIIKISPGDPTWLDSDDFFELGMDSLQATILRRRILASITKSGREFKELSPDFIFKCPTIDTLHGLLSGNASDSSRERADRMNEMVRKYVSNIRSLPPPTMAPTAIVLLTGSTGSLGSFLLAQLTALPHVDKVICLNRRISSPGFKDLRSRQEAALSKGGITIPTSSWGRITIHEADLKAPSFGLPDSDYNSLRSVTHIIHNAWPMDFNRSLESFEPHFHATQNIVSLALLANESNPQNKPRILFTSSVAAVGRYPALTGNALIPEDPMHNAQVTDHFGYPEAKWVCERLIEEAGRIHPGRIETASVRIGQLTGSESSGAWSPKEHFPSILKSSQTIGSLPDIKGTLSWIPVDRAARVVIELLFQSSPPDPIYHLENPMRQPWSDLLVLLSRALGLPSKIIPFEAWLDKVRSITDAEKNPAGKVVKFLEEEFTAMASGAVVLDTRKARRVSRTLAGSGGIESRHVDLYVKYWRSVGHLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>D5GA52</accession>
    <name>D5GA52_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00005135001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430075">
        <property type="protein sequence ID" value="CAZ81406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837215.1">
        <property type="nucleotide sequence ID" value="XM_002837169.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GA52"/>
    <dbReference type="STRING" id="656061.D5GA52"/>
    <dbReference type="GeneID" id="9187104"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005135001"/>
    <dbReference type="eggNOG" id="KOG2031">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007773_2_0_1"/>
    <dbReference type="InParanoid" id="D5GA52"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017005">
        <property type="term" value="F:3'-tyrosyl-DNA phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09122">
        <property type="entry name" value="PLDc_Tdp1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010347">
        <property type="entry name" value="Tdp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12415">
        <property type="entry name" value="TYROSYL-DNA PHOSPHODIESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12415:SF4">
        <property type="entry name" value="TYROSYL-DNA PHOSPHODIESTERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06087">
        <property type="entry name" value="Tyr-DNA_phospho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="178"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="584"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="191"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="591"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="314"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR610347-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR610347-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ81406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="603" mass="66561" checksum="F9457D49E91DE80C" modified="2010-06-15" version="1">MEHTYEISDSDSGANEDEERQIAEAIRLSLLESPHPPAQGDASGSRDPQRGAKKRKMDVIEIVSDDEEDDGILDGINATRKAPKASDNSSVRARAVGIPGVNGPAIVLEPADGLLRMIGNRAQMERERLARLAELKRSPPEFGLLLDGAAPEAPKPAEAAGRLSCFFGGGSVPNKASAFEPSGFSADNRKQINTRGTRQSGNDSDSPDATPRFLQYPNGVVRKTWADGYPKTDTEITFEEVLQKESLCVAVLSAFQWDIDWVLKKLPLDTIQRLVMVMHAKEEQDRSYKVQQLGSLPRTTLVLPPMQGQVSCMHSKLMLLFHMNGDQRWLRVAVPSANLTDYDWGELGGVMENTVFIIDLPRLPKPNHNQTHFAKELHHFCAAKGMPEDVLNGLYRYDFSRTKDMAFVHSIGGSNAGKDWRRTGYSGLGTAVKALGLSSGPGLEFDFVTSSLGAANMGFISNMYRAAMGDDGLKELQRCSNKSVKGAKKKSVLVMGDQITDGDSETGFDEETNQERIMNHVRIYFPSYETVRLSKKGFNSGGTICFNSSWWDSPTFPRSVMRDCVSVRDGLLMHNKMLFARPSRQLQASAGKRKRMVGKTSHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>D5GA58</accession>
    <name>D5GA58_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Maintenance of mitochondrial morphology protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">MMM1</name>
        <name evidence="12" type="ORF">GSTUM_00005145001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the ERMES/MDM complex, which serves as a molecular tether to connect the endoplasmic reticulum (ER) and mitochondria. Components of this complex are involved in the control of mitochondrial shape and protein biogenesis, and function in nonvesicular lipid trafficking between the ER and mitochondria. The MDM12-MMM1 subcomplex functions in the major beta-barrel assembly pathway that is responsible for biogenesis of all outer membrane beta-barrel proteins, and acts in a late step after the SAM complex. The MDM10-MDM12-MMM1 subcomplex further acts in the TOM40-specific pathway after the action of the MDM12-MMM1 complex. Essential for establishing and maintaining the structure of mitochondria and maintenance of mtDNA nucleoids.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer. Component of the ER-mitochondria encounter structure (ERMES) or MDM complex, composed of MMM1, MDM10, MDM12 and MDM34. A MMM1 homodimer associates with one molecule of MDM12 on each side in a pairwise head-to-tail manner, and the SMP-LTD domains of MMM1 and MDM12 generate a continuous hydrophobic tunnel for phospholipid trafficking.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endoplasmic reticulum membrane</location>
            <topology evidence="8">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <text evidence="8">The ERMES/MDM complex localizes to a few discrete foci (around 10 per single cell), that represent mitochondria-endoplasmic reticulum junctions. These foci are often found next to mtDNA nucleoids.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the MMM1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430075">
        <property type="protein sequence ID" value="CAZ81412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837221.1">
        <property type="nucleotide sequence ID" value="XM_002837175.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GA58"/>
    <dbReference type="FunCoup" id="D5GA58">
        <property type="interactions" value="63"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GA58"/>
    <dbReference type="GeneID" id="9185694"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005145001"/>
    <dbReference type="eggNOG" id="ENOG502QUUW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032730_1_0_1"/>
    <dbReference type="InParanoid" id="D5GA58"/>
    <dbReference type="OMA" id="WSFTQGL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032865">
        <property type="term" value="C:ERMES complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120013">
        <property type="term" value="F:lipid transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015917">
        <property type="term" value="P:aminophospholipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070096">
        <property type="term" value="P:mitochondrial outer membrane translocase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990456">
        <property type="term" value="P:mitochondrion-endoplasmic reticulum membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045040">
        <property type="term" value="P:protein insertion into mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd21671">
        <property type="entry name" value="SMP_Mmm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03103">
        <property type="entry name" value="Mmm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027537">
        <property type="entry name" value="Mmm1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019411">
        <property type="entry name" value="MMM1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13466:SF0">
        <property type="entry name" value="SMP-LTD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13466">
        <property type="entry name" value="TEX2 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10296">
        <property type="entry name" value="MMM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="6" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="7 8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="topological domain" description="Lumenal" evidence="8">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="8">
        <location>
            <begin position="52"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="SMP-LTD" evidence="11">
        <location>
            <begin position="113"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="316"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ81412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="353" mass="39490" checksum="11281BC532C7F663" modified="2010-06-15" version="1">MQQERHIQLPLSEYLALTTKQCSSWSFTQGLLLGQLIVILTLGCFIKFFIFGDSPGSTSKTIYSGSFTPTALKQFSRNQSNLRKKRSAVLRDPPSQSTETILSKTYYDVRSHQPESLDWFNVLIAQTLAQFREDAHTDGAILKSLNKFLNGPQKPDFLDSIRVTEVSLGEDFPIFSNCRISPSDDEPGKLQAKMDVDLSDLITLGVETKLLLNWPKPLVAVLPVALAVSIVRFSGTLSISFIPSPENLAGNTTLTFSFLDDYRLEIAVRSLVGSRSRLQDVPKIAQLVESRLHGWIDERCVEPKFQQIVLPSLWPRKRTTREQSRPGEGTDDGELPGGSFDGLRRRPQPTAHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAK9</accession>
    <name>D5GAK9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA-5-taurinomethyluridine 2-sulfurtransferase</fullName>
            <ecNumber evidence="3">2.8.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">GSTUM_00003665001</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the 2-thiolation of uridine at the wobble position (U34) of mitochondrial tRNA(Lys), tRNA(Glu) and tRNA(Gln). Required for the formation of 5-taurinomethyl-2-thiouridine (tm5s2U) of mitochondrial tRNA(Lys), tRNA(Glu), and tRNA(Gln) at the wobble position. ATP is required to activate the C2 atom of the wobble base.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>5-taurinomethyluridine(34) in tRNA + S-sulfanyl-L-cysteinyl-[protein] + AH2 + ATP = 5-taurinomethyl-2-thiouridine(34) in tRNA + L-cysteinyl-[protein] + A + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:47040"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11726"/>
            <dbReference type="Rhea" id="RHEA-COMP:11732"/>
            <dbReference type="Rhea" id="RHEA-COMP:11733"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61963"/>
            <dbReference type="ChEBI" id="CHEBI:87171"/>
            <dbReference type="ChEBI" id="CHEBI:87172"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.8.1.14"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MnmA/TRMU family.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.14" evidence="3"/>
    <dbReference type="EMBL" id="FN430079">
        <property type="protein sequence ID" value="CAZ81552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837361.1">
        <property type="nucleotide sequence ID" value="XM_002837315.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAK9"/>
    <dbReference type="FunCoup" id="D5GAK9">
        <property type="interactions" value="348"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GAK9"/>
    <dbReference type="GeneID" id="9187492"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003665001"/>
    <dbReference type="eggNOG" id="KOG2805">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035188_1_2_1"/>
    <dbReference type="InParanoid" id="D5GAK9"/>
    <dbReference type="OMA" id="LFMRNWN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103016">
        <property type="term" value="F:tRNA-uridine 2-sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990799">
        <property type="term" value="P:mitochondrial tRNA wobble position uridine thiolation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01998">
        <property type="entry name" value="MnmA_TRMU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.280">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004506">
        <property type="entry name" value="MnmA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046885">
        <property type="entry name" value="MnmA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046884">
        <property type="entry name" value="MnmA-like_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023382">
        <property type="entry name" value="MnmA-like_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00420">
        <property type="entry name" value="trmU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11933:SF5">
        <property type="entry name" value="MITOCHONDRIAL TRNA-SPECIFIC 2-THIOURIDYLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11933">
        <property type="entry name" value="TRNA 5-METHYLAMINOMETHYL-2-THIOURIDYLATE -METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03054">
        <property type="entry name" value="tRNA_Me_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20258">
        <property type="entry name" value="tRNA_Me_trans_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20259">
        <property type="entry name" value="tRNA_Me_trans_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="4" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="tRNA-specific 2-thiouridylase MnmA-like central" evidence="14">
        <location>
            <begin position="348"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA-specific 2-thiouridylase MnmA-like C-terminal" evidence="13">
        <location>
            <begin position="409"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="98"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="98"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF20258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF20259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAZ81552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="512" mass="57400" checksum="655E56B4EE27CD75" modified="2010-06-15" version="1">MNGQTLTRTSLRLFRLKPKPRKPVQAPFFISAIQRQHFQPFSAAEYSEPNVSPHDKIFIAMSSGVDSSVAAALLLRSGHPRENLLPFYMANWSPSANPDPIPPPPSLPGRARRNRASTRPQSNEGSLKCTEREFNDVKDVCRALDLPEPICMNFEKEYWCEVFVPMLEMYRCGKTPNPDVECNRHIKFGAVIKRLRDEFRRRWEGVTEKGSKWWLATGHYAHILYHIPTKSYHLLRSIDINKDQTFYLSTLHRDVLSRLLFPLGSYGLSKPGVKSLARELLLPGWRPGQTERPESMGLCFVEPAGGKGKTGFRRFLSGYLEPEPGDVVIGPSKKYPGGGESEGACAPEGSVVGKHAGLWHATIGERARFQLPQASPEYRGKWYVSRKNRELNTLEIVRGQDNARLYGKGMVVEKWRWLGDDAADIVRERSASNGRGLVTQFRHRQIPLKVSSVEQPGGPGYRPCSEGNEPAPALKIIFDSPAMAIAPGQSAALWFRDRCLGGGVIRDAIELD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAP7</accession>
    <name>D5GAP7_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphoinositide phospholipase C</fullName>
            <ecNumber evidence="7">3.1.4.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00003722001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + H2O = 1D-myo-inositol 1,4,5-trisphosphate + a 1,2-diacyl-sn-glycerol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33179"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:203600"/>
            <dbReference type="EC" id="3.1.4.11"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.1.4.11" evidence="7"/>
    <dbReference type="EMBL" id="FN430084">
        <property type="protein sequence ID" value="CAZ81590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837399.1">
        <property type="nucleotide sequence ID" value="XM_002837353.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAP7"/>
    <dbReference type="STRING" id="656061.D5GAP7"/>
    <dbReference type="GeneID" id="9187763"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003722001"/>
    <dbReference type="eggNOG" id="KOG0169">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002738_3_0_1"/>
    <dbReference type="InParanoid" id="D5GAP7"/>
    <dbReference type="OMA" id="FCFNRRF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004435">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate phospholipase C activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051209">
        <property type="term" value="P:release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00275">
        <property type="entry name" value="C2_PLC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08598">
        <property type="entry name" value="PI-PLC1c_yeast"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.190:FF:000039">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001192">
        <property type="entry name" value="PI-PLC_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000909">
        <property type="entry name" value="PLipase_C_PInositol-sp_X_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001711">
        <property type="entry name" value="PLipase_C_Pinositol-sp_Y"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336:SF82">
        <property type="entry name" value="PHOSPHOINOSITIDE PHOSPHOLIPASE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336">
        <property type="entry name" value="PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00388">
        <property type="entry name" value="PI-PLC-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00387">
        <property type="entry name" value="PI-PLC-Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00390">
        <property type="entry name" value="PHPHLIPASEC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00148">
        <property type="entry name" value="PLCXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00149">
        <property type="entry name" value="PLCYc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50008">
        <property type="entry name" value="PIPLC_Y_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="4 7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="PI-PLC Y-box" evidence="10">
        <location>
            <begin position="347"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="9">
        <location>
            <begin position="466"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="297"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="332"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00059664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ81590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="607" mass="67539" checksum="B5FE5ADB7EA3F4F1" modified="2010-06-15" version="1">MATATASHGSAGGGIATQALTAKPNKIDSKVLLGELQKVYTSLCPRGYSTLPVSVFKSYLSDTQKVATYDEHSGNEEDVKETGVQKWVHKVFGHSSQSFSAEKLLSDDQDITFDEFVSYHATPTNSALALPDSKEKDWSRPLNEYFISSSHNTYLTGHQLYGSSTTEGYINVLSRGCRCIEIDVWDGDDGEPEVFHGYTLTKEISFRDVCKAVAEHAFSEGGSLWQGGAGEGPVIISLECHAGPKQQEKIVKIMREQWGDMLVQGIEPENVENLPSPLELRRKILLTFQAKYLPPPPKATPIDVTAPRVPPPAVSDSSSDSSDSELEELAKKANKQKPPKAKVTRALAALGIYCSSHHFPSRAAQPFTEEPTSRIPNHVYSFSEKVFANLHAQHAESIFHHNKNYMMRVYPFGLRFSSSNADPTEFWQRGVQLVALNWQKCDEGMMLNEGMFAGEGGYVLKPDSYRPGGRGIHEDKRSLDLTIVVIAAVNIPLPEKSDSPKRFEPYVKIEVHTDAAPGLKKKTKAKRGIECTWNEKLEFKGISGIVEKLTFIRFKIHDEEFGRDDLAAWACIRLDRLQEGYRFVNLINNEGDPTNGLILVKISKRVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAS6</accession>
    <name>D5GAS6_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome b-c1 complex subunit 2, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Core protein II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00005280001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peptidase M16 family. UQCRC2/QCR2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430086">
        <property type="protein sequence ID" value="CAZ81619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837428.1">
        <property type="nucleotide sequence ID" value="XM_002837382.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAS6"/>
    <dbReference type="FunCoup" id="D5GAS6">
        <property type="interactions" value="264"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GAS6"/>
    <dbReference type="GeneID" id="9187786"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005280001"/>
    <dbReference type="eggNOG" id="KOG2583">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009902_0_1_1"/>
    <dbReference type="InParanoid" id="D5GAS6"/>
    <dbReference type="OMA" id="APKFALY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000021">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050361">
        <property type="entry name" value="MPP/UQCRC_Complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851:SF209">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851">
        <property type="entry name" value="METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="12">
        <location>
            <begin position="46"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="13">
        <location>
            <begin position="193"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ81619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="445" mass="46988" checksum="2E9F745FBEAC0688" modified="2010-06-15" version="1">MLARSTLSRGPSQLLARAAPSHRGLAATAASNPFHYRVGKSAGITVASRDDGGPTTTLAVVSRGGSRYETSPGLAHGLEKFAFKNSRRSALRLQRETELLGGSLGSTLSRENIVLRAKFLREDLPYFVEALADVLIHTKYNPYEFNEQVASTLNFEVEKLHHTPAALALEAAHSVAFHKGLGSSRLALTNKYLTSKSITEYSKEVYNKGNIAVVASGAPQLDLERWTAEFFKELPSGTGPVMAPAKYYGGENRLFSPHGNAIVIAFPGSSSPPSFKAEYTVLAYLLGGEASTKWNAGMSLLSQAISETPHTTAVAKHVAYTDTGLLYITIEGSGSAVTQAGGNVVSAIKSLGEAKPEDVKRAIALAKFDVLAAAEDRSAGLEAVGQAVIARGEAPQVEGVVKALDGVTVEAVKAVGKKLLDSKATFVAVGDTYLLPYAGDLGLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAV7</accession>
    <name>D5GAV7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00005327001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430086">
        <property type="protein sequence ID" value="CAZ81650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837459.1">
        <property type="nucleotide sequence ID" value="XM_002837413.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAV7"/>
    <dbReference type="STRING" id="656061.D5GAV7"/>
    <dbReference type="GeneID" id="9187818"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005327001"/>
    <dbReference type="eggNOG" id="KOG3410">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098435_1_1_1"/>
    <dbReference type="InParanoid" id="D5GAV7"/>
    <dbReference type="OMA" id="QLSEHHD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013865">
        <property type="entry name" value="FAM32A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13282">
        <property type="entry name" value="PROTEIN FAM32A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13282:SF6">
        <property type="entry name" value="PROTEIN FAM32A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08555">
        <property type="entry name" value="FAM32A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="43"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="126" mass="14402" checksum="3AEADFF32EDCC3EC" modified="2010-06-15" version="1">MGKEDYISTGGSLKLKGTKDGGIKKKKKKKPATPDPTSELKSRTASAKATSADGLEEREEEEREEPEDPYAGKTEAERRFEERKRKRMDERLKKEGIKSHKERVEEFNKYLSNLSEHHDMPRIGPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBD0</accession>
    <name>D5GBD0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein PBDC1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00000432001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PBDC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430091">
        <property type="protein sequence ID" value="CAZ81823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837632.1">
        <property type="nucleotide sequence ID" value="XM_002837586.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBD0"/>
    <dbReference type="FunCoup" id="D5GBD0">
        <property type="interactions" value="617"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GBD0"/>
    <dbReference type="GeneID" id="9184396"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000432001"/>
    <dbReference type="eggNOG" id="KOG4093">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103791_1_0_1"/>
    <dbReference type="InParanoid" id="D5GBD0"/>
    <dbReference type="OMA" id="IQFYAFE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051787">
        <property type="term" value="F:misfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990593">
        <property type="term" value="F:nascent polypeptide-associated complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061770">
        <property type="term" value="F:translation elongation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051083">
        <property type="term" value="P:'de novo' cotranslational protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3560.10:FF:000001">
        <property type="entry name" value="Protein PBDC1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3560.10">
        <property type="entry name" value="yst0336 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023139">
        <property type="entry name" value="PBDC1-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008476">
        <property type="entry name" value="PBDC1_metazoa/fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021148">
        <property type="entry name" value="Polysacc_synth_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13410">
        <property type="entry name" value="PROTEIN PBDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13410:SF9">
        <property type="entry name" value="PROTEIN PBDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04669">
        <property type="entry name" value="PBDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Polysaccharide biosynthesis" evidence="5">
        <location>
            <begin position="18"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00061201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00069779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ81823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="163" mass="19359" checksum="83EC4266144E7694" modified="2010-06-15" version="1">MSIPKDFRAEQAQNLQDIEKQFAVKAVIHAQTYWSILERRAGTDLRLTRMDQEIYDHFFETFPDFDPKKPLSEDEMKGKAGKEKWRNFLMKYERRVGDYNFGTLLRTDAKVEYEKDTTIFVPRMQFYAIEIARNRSGLNDWIKKAYDKEGEAKETKAVKENTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBD1</accession>
    <name>D5GBD1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00000469001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430092">
        <property type="protein sequence ID" value="CAZ81824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837633.1">
        <property type="nucleotide sequence ID" value="XM_002837587.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBD1"/>
    <dbReference type="FunCoup" id="D5GBD1">
        <property type="interactions" value="432"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GBD1"/>
    <dbReference type="GeneID" id="9184413"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000469001"/>
    <dbReference type="eggNOG" id="KOG0094">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_10_2_1"/>
    <dbReference type="InParanoid" id="D5GBD1"/>
    <dbReference type="OMA" id="PVSNDGC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01861">
        <property type="entry name" value="Rab6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000229">
        <property type="entry name" value="Probable Ras-related protein Rab-6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0636">Prenylation</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ81824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="208" mass="23395" checksum="AC89689BFDD4D74F" modified="2010-06-15" version="1">MAASAGSYSNPLKKFKLVFLGEQSVGKTSLITRFMYDSFDNMYQATIGIDFLSKTMYLEDRTVRLQLWDTAGQERFRSLIPSYIRDSSVAVVVYDITNRTSFQNTTKWVDDVRGERGNDVIIVLVGNKTDLNDKRQVTTEEGEKKAKEFKVMFIETSAKAGHNVKTLFKRIAQALPGMEGTLNEPNSEMINVDIDRNDSTQADGSCSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBG4</accession>
    <name>D5GBG4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00000462001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430094">
        <property type="protein sequence ID" value="CAZ81857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837666.1">
        <property type="nucleotide sequence ID" value="XM_002837620.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBG4"/>
    <dbReference type="STRING" id="656061.D5GBG4"/>
    <dbReference type="GeneID" id="9184431"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000462001"/>
    <dbReference type="eggNOG" id="KOG1245">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_525991_0_0_1"/>
    <dbReference type="InParanoid" id="D5GBG4"/>
    <dbReference type="OMA" id="YANACIW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028941">
        <property type="entry name" value="WHIM2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32075">
        <property type="entry name" value="ISWI CHROMATIN-REMODELING COMPLEX SUBUNIT YPL216W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32075:SF6">
        <property type="entry name" value="ISWI CHROMATIN-REMODELING COMPLEX SUBUNIT YPL216W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15613">
        <property type="entry name" value="WSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WHIM2" evidence="4">
        <location>
            <begin position="279"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="156"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="477"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="7"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="188"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="204"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF15613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ81857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="518" mass="59851" checksum="48D8433455BE1B93" modified="2010-06-15" version="1">MEIEDDEKSRAKSVDRERSHRADELFEDSDGWIQRLKVRDFKNGGWQAIVVGVLYRLSVDDRYSKDCEEILRHLTPVGEEPALESVRQRYLTLDINLRAMVLHLLSRLTHETRSVRDYMEECSEEMTKHRKDKIEVQRARRDLISELKMLDDERKILLPENTPRSPSPVAASRQTSTDADGDSKMTGADEEDLDGDDDEDGDDTDRGRSLRRGGERANERKRKREAEKEKADAAAKLPKTSKQFQKVLKTIESVKAKIAKQEEKIASFDEDLRQADCARFRMMGRDRFWNRYWWFERNGMPFGGLPQSSTAYAGYANAMIWVQGPDPSEREGFLVEEAGTKWASEEGSDMRMKMNGSNDGRMTVLERQALEESSTTLTSPTQYGYIDTVEEFEQMLSWLDIKGVREKKLKQQFEEYRKHIEEGMVNRKKYLAGESEGAPSMEAGSTRMNTRGKTHIDPEIWRCLNWTNETMIDEHGHTHFEQPQPRKASKKKGSAQEKGVPLNRQGKPVSRQGDRYSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBK1</accession>
    <name>D5GBK1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00005672001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ82007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837816.1">
        <property type="nucleotide sequence ID" value="XM_002837770.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBK1"/>
    <dbReference type="GeneID" id="9185442"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005672001"/>
    <dbReference type="HOGENOM" id="CLU_2759636_0_0_1"/>
    <dbReference type="InParanoid" id="D5GBK1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="70" mass="8018" checksum="F93757E8166B9DCD" modified="2010-06-15" version="1">MAMYDMTCMYDTPGYYFFTQPSVDQGRAGFLPSFLFVFFIFFVYDISSSKSGRGKQIATYQSRTCSSTHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBR5</accession>
    <name>D5GBR5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00005538001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ81915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837724.1">
        <property type="nucleotide sequence ID" value="XM_002837678.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBR5"/>
    <dbReference type="STRING" id="656061.D5GBR5"/>
    <dbReference type="GeneID" id="9182952"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005538001"/>
    <dbReference type="eggNOG" id="KOG4563">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028900_1_0_1"/>
    <dbReference type="InParanoid" id="D5GBR5"/>
    <dbReference type="OMA" id="IAECHYK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034080">
        <property type="term" value="P:CENP-A containing chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006335">
        <property type="term" value="P:DNA replication-dependent chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051730">
        <property type="entry name" value="NASP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019544">
        <property type="entry name" value="Tetratricopeptide_SHNi-TPR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15081:SF1">
        <property type="entry name" value="NUCLEAR AUTOANTIGENIC SPERM PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15081">
        <property type="entry name" value="NUCLEAR AUTOANTIGENIC SPERM PROTEIN NASP -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10516">
        <property type="entry name" value="SHNi-TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="Tetratricopeptide SHNi-TPR" evidence="4">
        <location>
            <begin position="242"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="76"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="179"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="424"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="77"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="130"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="179"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="197"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="216"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="427"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="457"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="467"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ81915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="496" mass="52939" checksum="B0D74FE66E40F28B" modified="2010-06-15" version="1">MSTETTTTRLATLKAQAAKSYAQKDYSAASDSYAQACELQSSINGDDNPQNAHLLYLYGRSLFQVALTKSDVLGGKPVEEQDEKEKKGGIAKGKGKDVSEAGGEGNDEKLKAPAPVVGAKGGFFSFTGDENWDDSEEEGEDADAGGDGDVEKEGDDDFTLAWEILDFARVLFLKQLGSSKTADDQTTGTNEVEPIVQETTTDTSNDPITSIPNPDSALPAASSASTSTETKPDFKTLKTALADTYDLLGEVSLESESFPQATKDLRSSLELKLKLYPPSSTLISEAHFKLSLALEFAAAGEGVTNDDRRKGREEAAVQMELAIASCRARITKEEAELKELGTDGDKDGKAKKSLEEAREMVGELEVRLADLRSATPSPTLADAQDDDQLRGILGQVLGEDPEEARKRIAEVVGRANDLSGLVRKKPASASAEPAPATAGASTVIVPINGGKRKLEDVVEEEPVEELEIPKKVKLQDEAKPEAEAEEKVEGEGDVKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBS1</accession>
    <name>D5GBS1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00005547001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ81921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837730.1">
        <property type="nucleotide sequence ID" value="XM_002837684.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBS1"/>
    <dbReference type="STRING" id="656061.D5GBS1"/>
    <dbReference type="GeneID" id="9182958"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005547001"/>
    <dbReference type="eggNOG" id="ENOG502RXQH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022884_1_0_1"/>
    <dbReference type="InParanoid" id="D5GBS1"/>
    <dbReference type="OMA" id="MVATMNS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901135">
        <property type="term" value="P:carbohydrate derivative metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05014">
        <property type="entry name" value="SIS_Kpsf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035474">
        <property type="entry name" value="SIS_Kpsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38418">
        <property type="entry name" value="SUGAR ISOMERASE, KPSF/GUTQ (AFU_ORTHOLOGUE AFUA_6G08860)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38418:SF2">
        <property type="entry name" value="SUGAR ISOMERASE, KPSF_GUTQ (AFU_ORTHOLOGUE AFUA_6G08860)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SIS" evidence="1">
        <location>
            <begin position="94"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="437" mass="46407" checksum="8BF2AFDE2E545986" modified="2010-06-15" version="1">MDNGRSNSDFFRHLRDGRNNLPFAQHLSCLPNTEAISSLPITPPTTYADPEDEEVLSTAVRVLSTEAMALSCLSQLYATNPVARDGFVRAVEAISTAVVKGGKVIIIGVGKSGKIGEKMVATMNSLGLLSVFMHPIEALHGDLGMVKPKDVLLLITFSGNTPELISLLPHLPGHLPLIALTSHISYHTSPLTRERKKAILLPAPIHEAEETSFGISAPTTSTTVALALGDALAVVSARHVYTGDGEKPKDVFKRNHPGGAIGLGTGKGVVRVSDLAVRLEDIPVVDGILEDGLLDSPPISSTSSESDLEITEWTDTPLSATHSVRVIDCLLAGVGSKTGWVRVSNDQVIPPGRLKSIRGDVQGGVYEFEPSLVVAKSEMIRVSGQANAEDMKKWVMASREEERGVFPAGAVLGVCVGDAGKGEEVIAVIEVDELMRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>D5GC10</accession>
    <name>D5GC10_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00005754001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430098">
        <property type="protein sequence ID" value="CAZ82053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837862.1">
        <property type="nucleotide sequence ID" value="XM_002837816.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GC10"/>
    <dbReference type="STRING" id="656061.D5GC10"/>
    <dbReference type="GeneID" id="9181322"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005754001"/>
    <dbReference type="eggNOG" id="ENOG502QWJN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_364846_0_0_1"/>
    <dbReference type="InParanoid" id="D5GC10"/>
    <dbReference type="OMA" id="MQLQPED"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013877">
        <property type="entry name" value="YAP-bd/ALF4/Glomulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040347">
        <property type="entry name" value="YBP1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28020">
        <property type="entry name" value="YAP1-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28020:SF1">
        <property type="entry name" value="YAP1-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08568">
        <property type="entry name" value="Kinetochor_Ybp2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="184"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="326"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="199"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="613" mass="67025" checksum="68F6E0EB358DA4F6" modified="2010-06-15" version="1">MERIVTLDKAINAIKTAAEEIPADDFISYSTILDVHLSYAHDAFSQDEQHRLLEVLGDILETHEDLAREISWDLISVLLPFLESLSPATVQLTESHIAFAAEKGNPREGGEVGETEDKLRKETTRNALRKFHALLSALSILTTLLGVFTKAVQCLDRQAVDGVLRGLLEFAELVKPALAAPRKQSIVKGERPPLPPRVPTNSTNPSLAPTSADGDAPAKEPEGDSPETTLQARLLQSFLSHLLEIYLLRTRDITGRGLVVGWAGQYDSEVVRKGKSEVPGGKTPIDDERAERAVQRDVKALTEDIEGLCEALGLRADELMKICESHQEPESPTDEDQEDPAPPSSAEDIPLSKTGALFLLAQRLAHYPLSSLSIFPNHSDISQNFLVNGSGQHQPAVVDAVLFLGALALHQGGGLGNAPELDDFLLYLQTFAAISATSSSPQARFLAHYHVSTCISKHPDEAARLAYIKDTLEHCPFETLKAAVVGILKDEIQSASLSTPPKPNSIFASPVCLEELFNVLFLEVEIGDFDDFKNYYPTLVATANLYYFLVLSPVCREKLGVGKREFAERVEEKFLTSVGKAVEGISPEGGGMETDILKDVIERIREKVKEVCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCM3</accession>
    <name>D5GCM3_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Octanoyltransferase</fullName>
            <ecNumber evidence="5">2.3.1.181</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00000727001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>octanoyl-[ACP] + L-lysyl-[protein] = N(6)-octanoyl-L-lysyl-[protein] + holo-[ACP] + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17665"/>
            <dbReference type="Rhea" id="RHEA-COMP:9636"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:9928"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78463"/>
            <dbReference type="ChEBI" id="CHEBI:78809"/>
            <dbReference type="EC" id="2.3.1.181"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 5">Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the LipB family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.181" evidence="5"/>
    <dbReference type="EMBL" id="FN430114">
        <property type="protein sequence ID" value="CAZ82266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838075.1">
        <property type="nucleotide sequence ID" value="XM_002838029.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCM3"/>
    <dbReference type="FunCoup" id="D5GCM3">
        <property type="interactions" value="378"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GCM3"/>
    <dbReference type="GeneID" id="9187885"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000727001"/>
    <dbReference type="eggNOG" id="KOG0325">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035168_1_2_1"/>
    <dbReference type="InParanoid" id="D5GCM3"/>
    <dbReference type="OMA" id="TEEPMWY"/>
    <dbReference type="UniPathway" id="UPA00538">
        <property type="reaction ID" value="UER00592"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033819">
        <property type="term" value="F:lipoyl(octanoyl) transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000544">
        <property type="entry name" value="Octanoyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020605">
        <property type="entry name" value="Octanoyltransferase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00214">
        <property type="entry name" value="lipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10993:SF7">
        <property type="entry name" value="LIPOYLTRANSFERASE 2, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10993">
        <property type="entry name" value="OCTANOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21948">
        <property type="entry name" value="LplA-B_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016262">
        <property type="entry name" value="LPLase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01313">
        <property type="entry name" value="LIPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="9">
        <location>
            <begin position="52"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="6">
        <location>
            <position position="197"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="99"/>
            <end position="106"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="166"/>
            <end position="168"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="179"/>
            <end position="181"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Cys" evidence="8">
        <location>
            <position position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR016262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR016262-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR016262-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR016262-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ82266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="254" mass="27957" checksum="0E886D9B866EE38B" modified="2010-06-15" version="1">MSRTRLLHIHLGKTTYASAAALQKSLLSAHLSHKSRTTLIPISPLPTLITTQPPEPTVITSEFHPPVYTLGRRESQSPLTPERESHLRVRGAELQYTPRGGLTTFHGPGQLTAYVIADLRHHGLRPRCYIRTLERALVATCAQWGIRAFTTEDTGAWTSENRKIGAVGVHMRRFVSSFGVGLNVSTDLWWFDRIVACGLEGKGTTSFVREGVVGVGVEEVGDRFVKAMAESFGCTSIGKKSRKDIPPDLLVEES</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>D5GD42</accession>
    <name>D5GD42_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00000961001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430139">
        <property type="protein sequence ID" value="CAZ82435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838244.1">
        <property type="nucleotide sequence ID" value="XM_002838198.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GD42"/>
    <dbReference type="STRING" id="656061.D5GD42"/>
    <dbReference type="GeneID" id="9183899"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000961001"/>
    <dbReference type="eggNOG" id="ENOG502S1YN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024412_1_1_1"/>
    <dbReference type="InParanoid" id="D5GD42"/>
    <dbReference type="OMA" id="FDEYMHP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="entry name" value="Leucine Aminopeptidase, subunit E, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012664">
        <property type="entry name" value="CHP02452"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019261">
        <property type="entry name" value="PARG_cat_microbial"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02452">
        <property type="entry name" value="TIGR02452 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35596">
        <property type="entry name" value="DUF2263 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35596:SF2">
        <property type="entry name" value="MICROBIAL-TYPE PARG CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10021">
        <property type="entry name" value="PARG_cat_microb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF014899">
        <property type="entry name" value="UCP014899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Microbial-type PARG catalytic" evidence="1">
        <location>
            <begin position="3"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="210" mass="23703" checksum="391E219A5F5B7DB7" modified="2010-06-15" version="1">MSNRILVLNTANEKKPGGEWEGGVLSQEEGFARRSNLIQALTTTDPRSGLQTYYPLENTSGIYSPNVVVFREGFDKDYELWRDEEWTTLAIVSAPAVRRPKVDESGLHYSFTEERQLQREKMKSVLRIAALNGHTNLVLGGFGSCGPEGSGGDLYKNPVRDVCLLWKDLLFEDEEFKGWFKNVVFAFGKGGGSWMKEDGNSIEEFRQFFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5GD88</accession>
    <name>D5GD88_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">aminodeoxychorismate synthase</fullName>
            <ecNumber evidence="4">2.6.1.85</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Para-aminobenzoate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">p-aminobenzoic acid synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00006093001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>chorismate + L-glutamine = 4-amino-4-deoxychorismate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:11672"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58406"/>
            <dbReference type="EC" id="2.6.1.85"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 4-aminobenzoate from chorismate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the anthranilate synthase component I family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.85" evidence="4"/>
    <dbReference type="EMBL" id="FN430142">
        <property type="protein sequence ID" value="CAZ82481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838290.1">
        <property type="nucleotide sequence ID" value="XM_002838244.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GD88"/>
    <dbReference type="FunCoup" id="D5GD88">
        <property type="interactions" value="166"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GD88"/>
    <dbReference type="GeneID" id="9183945"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006093001"/>
    <dbReference type="eggNOG" id="KOG1224">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006493_0_0_1"/>
    <dbReference type="InParanoid" id="D5GD88"/>
    <dbReference type="OMA" id="DWSVNIR"/>
    <dbReference type="UniPathway" id="UPA00077">
        <property type="reaction ID" value="UER00149"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046820">
        <property type="term" value="F:4-amino-4-deoxychorismate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008153">
        <property type="term" value="P:4-aminobenzoate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01743">
        <property type="entry name" value="GATase1_Anthranilate_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.120.10">
        <property type="entry name" value="Anthranilate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005801">
        <property type="entry name" value="ADC_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019999">
        <property type="entry name" value="Anth_synth_I-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006805">
        <property type="entry name" value="Anth_synth_I_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015890">
        <property type="entry name" value="Chorismate_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010117">
        <property type="entry name" value="PabB_fungal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006221">
        <property type="entry name" value="TrpG/PapA_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01823">
        <property type="entry name" value="PabB-fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00566">
        <property type="entry name" value="trpG_papA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11236">
        <property type="entry name" value="AMINOBENZOATE/ANTHRANILATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11236:SF18">
        <property type="entry name" value="AMINODEOXYCHORISMATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04715">
        <property type="entry name" value="Anth_synt_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00425">
        <property type="entry name" value="Chorismate_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00097">
        <property type="entry name" value="ANTSNTHASEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00096">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56322">
        <property type="entry name" value="ADC synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase" evidence="11">
        <location>
            <begin position="4"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="Anthranilate synthase component I N-terminal" evidence="13">
        <location>
            <begin position="271"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="domain" description="Chorismate-utilising enzyme C-terminal" evidence="12">
        <location>
            <begin position="471"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="435"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ82481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="745" mass="82390" checksum="74CD9F1D982F071E" modified="2010-06-15" version="1">MRILLIDAYDSFAQNLAQLLTSATGATVYTIRIDDFADISFLKPHLPSFHAVVIGPGPGSPEVEADIGVVNDVWKLSGGDVLPVFGVCLGLQSLVFSHGGRVNRLGTVKHGLLSEIVHTGEGLFEGVGKVNVVRYHSLHGITKIGGDVEEIAWAYDENGRVVMGVKHISKPFWGVQYHPESVCSEDGRDVVGNFWKMAMDWNTGQGRVVKELRTDWRGQSREKGLLELQTTLEGRRLDDCQKVEVRTEDIFGKGISAGRICEILGVEAANEFILLESAAAPGRFSIIGVLTPGLTQRINYTCGDTFLKLSYTGRDSSETRIDLRTYGGSIWRFLAKYMDQRKAVGGKDHSPFWGGLVGYFNYETGVNSLNIPIKPRKEAEGMKRRPDVNLAFVERSIVLDGHTGRVYIQTLLPPAADGNWLESTKRQLQPEADMAITPSATPPPEPSPECHRSFLETSHPKPSPVIYKPDAERYMQKVRTCQSYLSAGDSYELCLTATTKIHLEPTSPWALYRHLCARNPAPYASYFRLSNVTLLSSSPERFLSWSRTGACRLCPIKGTVRKTPTTTYSSASLQLNTPKERAENLMIVDLIRHDLHRLATNVSVEKLMVVEEYASVYQLVSVITGSVEPQDNMTGFDVLARSLPPGSMTGAPKKRSVEILQEIEETERGVYSGVLGYWSVCGAGDWNVIIRSAFRYDDEVVTMEDGGEKEVWRIGAGGAVTALSGPREEWEEMEVKLGEPCSLFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDE4</accession>
    <name>D5GDE4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00006172001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430142">
        <property type="protein sequence ID" value="CAZ82537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838346.1">
        <property type="nucleotide sequence ID" value="XM_002838300.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDE4"/>
    <dbReference type="STRING" id="656061.D5GDE4"/>
    <dbReference type="GeneID" id="9184000"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006172001"/>
    <dbReference type="eggNOG" id="KOG0080">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_10_6_1"/>
    <dbReference type="InParanoid" id="D5GDE4"/>
    <dbReference type="OMA" id="IRYCDDQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001447">
        <property type="entry name" value="Ras-related protein Rab-1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="164"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ82537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="186" mass="20295" checksum="1DAC50C4D986A913" modified="2010-06-15" version="1">MADPTIKLLLIGPSSSGKTALLLRYVEDIFDTDNATATIGVDFKVKKLSVKGKLHRVFLFDTAGQERFRTLTSSYYRNAQGEVEKYAPPDAVKCLVGSKLDKSTSSRIVRTEEGRALADRYSASFYEVSSKTRENVKEPFVDTVTRIVETPVLVNGGAGAKRGTTALSPPVVSFNQREPPPKSGCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDI9</accession>
    <name>D5GDI9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00001046001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430145">
        <property type="protein sequence ID" value="CAZ82582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838391.1">
        <property type="nucleotide sequence ID" value="XM_002838345.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDI9"/>
    <dbReference type="STRING" id="656061.D5GDI9"/>
    <dbReference type="GeneID" id="9188171"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001046001"/>
    <dbReference type="eggNOG" id="KOG0151">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007957_0_0_1"/>
    <dbReference type="InParanoid" id="D5GDI9"/>
    <dbReference type="OMA" id="VWYRWKL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.790">
        <property type="entry name" value="Surp module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006569">
        <property type="entry name" value="CID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051485">
        <property type="entry name" value="SR-CTD_assoc_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000061">
        <property type="entry name" value="Surp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035967">
        <property type="entry name" value="SWAP/Surp_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23140">
        <property type="entry name" value="RNA PROCESSING PROTEIN LD23810P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23140:SF0">
        <property type="entry name" value="U2 SNRNP-ASSOCIATED SURP MOTIF-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01805">
        <property type="entry name" value="Surp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00648">
        <property type="entry name" value="SWAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109905">
        <property type="entry name" value="Surp module (SWAP domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51391">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50128">
        <property type="entry name" value="SURP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="SURP motif" evidence="3">
        <location>
            <begin position="366"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="CID" evidence="4">
        <location>
            <begin position="504"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="55"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="161"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="815"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="55"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="184"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ82582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="843" mass="91597" checksum="F6B50A16476296E4" modified="2010-06-15" version="1">MADSHDPFPNVKEKLTARTKKSAFEKSRLEAEAKRLREEAETAAVYKDFVASFEQEPSCSTPGASNLSGTRGSLGNSRDPTGDSRGGFRGIPPSGPGRRHFTSAPARGSQAAILLSGPQNRKRNIDSLFEREEEVGVFGSACLSEREKRRLRESNIGLLAFENSAPPSRSKDQYSHDDDSDSDPATNTHPKPTLQMTSLPPSTTKLTLTALFSATALKLDSIRIIPAPPPPGPSQPSPSVRKAASAIVMLSPETPSSDIDAAVSMLNGRYLGCGFWLGIGRHLPSTAVGTVGSGPIPAALQTYPFGARPPLAASRSLSRAPPVHRGGFASAFGSSHRGVSTGTSAGGAGNLLVQVEAPSDLKVLRLIHKTIESVLTHGPEFEALLMSSNIVKNDVKFSWLWDARSPEGVYYRWKLWEIISGTPREQIGGVDMFEPSSSLTTIWSPPRRMLKYEWAGTLRDVIEDEGFSSEELEDDRDDSDREEEIERPIGLMGIGEERRGRGYLGVLERAKLIHLISRIPTQTSKVRRGDVGRVMAFAMEHAEGGMGEEVVTVLVGNVVKPLAFSKAAKRENTPDGDDYNEVGSENNEGNLDTSAAKVIGLWLISDILSNSSLGIRNAWRYRQLFDVALREKGVFSHLGGVWKGSGWGRMKAEKFRRGVVEGVLEWWEKWCIFPQYTQEEFLRAFTEPVDREKTICAPGSQPPTEGTSATAPVVLTTKSKWKAVEAKAEAFNVESAVLATAEDVDGEPMADDDVDGESMVEDVDGEPMIDEEAEMEPETSDNHDGLILGRNVEAMSNSCGKPATGVIMQGFKIGSTSSTVKGGMGPRKRMRAEDMFADEDDRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDS1</accession>
    <name>D5GDS1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">UDP-glucose 4-epimerase</fullName>
            <ecNumber evidence="11">5.1.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00006245001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Mutarotase converts alpha-aldose to the beta-anomer. It is active on D-glucose, L-arabinose, D-xylose, D-galactose, maltose and lactose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>UDP-alpha-D-glucose = UDP-alpha-D-galactose</text>
            <dbReference type="Rhea" id="RHEA:22168"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="EC" id="5.1.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 11">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Carbohydrate metabolism; galactose metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Carbohydrate metabolism; hexose metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the NAD(P)-dependent epimerase/dehydratase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">In the C-terminal section; belongs to the aldose epimerase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">In the N-terminal section; belongs to the NAD(P)-dependent epimerase/dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.2" evidence="11"/>
    <dbReference type="EMBL" id="FN430153">
        <property type="protein sequence ID" value="CAZ82664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838473.1">
        <property type="nucleotide sequence ID" value="XM_002838427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDS1"/>
    <dbReference type="STRING" id="656061.D5GDS1"/>
    <dbReference type="GeneID" id="9182355"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006245001"/>
    <dbReference type="eggNOG" id="KOG1371">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_1_10_1"/>
    <dbReference type="InParanoid" id="D5GDS1"/>
    <dbReference type="OMA" id="GEHLICN"/>
    <dbReference type="UniPathway" id="UPA00214"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003978">
        <property type="term" value="F:UDP-glucose 4-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006012">
        <property type="term" value="P:galactose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd05247">
        <property type="entry name" value="UDP_G4E_1_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005886">
        <property type="entry name" value="UDP_G4E"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01179">
        <property type="entry name" value="galE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007956">
        <property type="entry name" value="PRK10675.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43725">
        <property type="entry name" value="UDP-GLUCOSE 4-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43725:SF47">
        <property type="entry name" value="UDP-GLUCOSE 4-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-0299">Galactose metabolism</keyword>
    <keyword evidence="7 11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5 11" id="KW-0520">NAD</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="12">
        <location>
            <begin position="8"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU366046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ82664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="367" mass="40402" checksum="E5ACF38912EF3270" modified="2010-06-15" version="1">MSAKEGTVLVTGGTGYIGSFTTLELLVAGYEVIVIDNLYNSSEESLNRIERICGKRPIFYKADVTDQEALDKIFKDHPDIDSVIHFAALKSVGESTEIPVEYYRVNVGGTISLLRSMEANGVKNIVFSSSATVYGDATRYPNMIPIPETCPIGPTNPYGRTKMMIEMVIEDHVESHPGWNAALLRYFNPAGAHPSGIMGEDPQGVPYNLLPLLGQVAVGRREKLKVFGTDYASHDGTAIRDYIHVVDLAKGHIAALNKLRKDKPGCRAWNLGTGKGSTVFEMIGAFSKAVGRDLPYEVVPRRKGDVLDLTAVPTRANEELGWKAEMTLEQACEDLWKWVSNNPQGYRQMPPQMLLDALAETRTKLPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDY7</accession>
    <name>D5GDY7_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ATP synthase subunit a</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">F-ATPase protein 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00001101001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATPase A chain family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430155">
        <property type="protein sequence ID" value="CAZ82730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838539.1">
        <property type="nucleotide sequence ID" value="XM_002838493.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDY7"/>
    <dbReference type="FunCoup" id="D5GDY7">
        <property type="interactions" value="229"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GDY7"/>
    <dbReference type="GeneID" id="9184525"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001101001"/>
    <dbReference type="eggNOG" id="KOG4665">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041018_0_2_1"/>
    <dbReference type="InParanoid" id="D5GDY7"/>
    <dbReference type="OMA" id="FFDQFMS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00310">
        <property type="entry name" value="ATP-synt_Fo_a_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.220:FF:000003">
        <property type="entry name" value="ATP synthase subunit a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.220">
        <property type="entry name" value="ATP synthase, F0 complex, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01393">
        <property type="entry name" value="ATP_synth_a_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000568">
        <property type="entry name" value="ATP_synth_F0_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045083">
        <property type="entry name" value="ATP_synth_F0_asu_bact/mt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035908">
        <property type="entry name" value="F0_ATP_A_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01131">
        <property type="entry name" value="ATP_synt_6_or_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11410">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11410:SF0">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00119">
        <property type="entry name" value="ATP-synt_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00123">
        <property type="entry name" value="ATPASEA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81336">
        <property type="entry name" value="F1F0 ATP synthase subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="5" id="KW-0138">CF(0)</keyword>
    <keyword evidence="7" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="132"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="179"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="208"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ82730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="242" mass="26579" checksum="5392B24794DDA163" modified="2010-06-15" version="1">MLSFKNITSPMDQFEVRNLLSLDVPLLGNINMSITNIGLYLIIESIYATIHSIVVSQINAAKGQIYFPFIYSLFIFILINNLLGMVEASPYDNIDNIFTFASTSHFILTFSISFTIVLGATLLGFQKHGLGFFSLFVPAGCPLALAYYLFLVLIEFISYLSRAISLGLRLAPNIVSGHLLLAILSGLTYNIMATGLLSFIIGLLPLSFIIAFCGLEIAIAFIQAQVFVVLTASYIKDALDLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDY8</accession>
    <name>D5GDY8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00001104001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430155">
        <property type="protein sequence ID" value="CAZ82731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="tml:GSTUM_00001104001"/>
    <dbReference type="HOGENOM" id="CLU_3435993_0_0_1"/>
    <dbReference type="InParanoid" id="D5GDY8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ82731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="13" mass="1567" checksum="C22164257964D736" modified="2010-06-15" version="1">MYSLTQLAYNCYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>D5GED3</accession>
    <name>D5GED3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00001275001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430174">
        <property type="protein sequence ID" value="CAZ82876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838685.1">
        <property type="nucleotide sequence ID" value="XM_002838639.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GED3"/>
    <dbReference type="STRING" id="656061.D5GED3"/>
    <dbReference type="GeneID" id="9185532"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001275001"/>
    <dbReference type="eggNOG" id="ENOG502S4ZA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_441590_0_0_1"/>
    <dbReference type="InParanoid" id="D5GED3"/>
    <dbReference type="OMA" id="WASCHRE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="410"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="225"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="357"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="557"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="46"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="271"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="291"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="357"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="367"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ82876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="619" mass="66447" checksum="CBEF943F0E67B600" modified="2010-06-15" version="1">MVSSNSTVDSAHGPQKMDHSPAEHNEPGDSREETGAPAGPSDKQKSLVVKLPLPKLSQPKENPPVIDLTDPTPEPQSINPSPNPESAATNLREAQKTKQTSPEPSVMPAKTPKPEIILAEQPVSHIRRSIVGRRSRYNPDTIVMDLLRTIGRHPTLPALNSGLEVLKDTLPNYFSGPVDLTTVRWDILDPPPPFAHNDETEDEAEILHRSLGAVGVGGGLVGVVASPAPAPRKRGRKKKGDVASSVPGHAPLASTGTPFPRPRGRPRKGTSITPIRSTIINVSGDAGGPSNKASFTQPTGASVSGAGVPGEGPSPRIGSSEAGGRQRASVGASGLRNVSTPSGSSIAVLIPPLSGASKSEYLTTPSNDRKRKSEERAEGGRAGKTPKLSVKSRSQKSGRPEPSGFKIFECRWKHCRARLHNFENLRKHVFKAHKKLTTYNAYACLWASCHREPPEEMKKRAIELGTILPDKISHDFKIGEEWEAHIDENHLAQVKEELGLGPAVLPSDCESGISDVDYMSDRDGHPVTPLAVLAPPGYRFTPPPGFPVNPQFRLAHGLPTPPARRRRNATGREGALGVVRNLAAGSSDPEKPDDDDDEGWKADQIPVVEEEDVQMREGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEH6</accession>
    <name>D5GEH6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00006487001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430175">
        <property type="protein sequence ID" value="CAZ82919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838728.1">
        <property type="nucleotide sequence ID" value="XM_002838682.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEH6"/>
    <dbReference type="GeneID" id="9181550"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006487001"/>
    <dbReference type="eggNOG" id="ENOG502SCZX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_462461_0_0_1"/>
    <dbReference type="InParanoid" id="D5GEH6"/>
    <dbReference type="OMA" id="QDEQMRS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="19"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="506"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="388"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="31"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="64"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="149"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="200"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="506"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ82919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="590" mass="63406" checksum="10B7934A69D33F6B" modified="2010-06-15" version="1">MATRHLPIRDLHRTNAIMLPDNPIPLPPPDDTSATATTASITPTSISPSVDSPSPTVQVTPKRPIASIPPASLAPAVSFTRPPATDPPISSPLAPHQGNSWDLETSYPGNGARFTSIPITPPRGSAQSNTRRLPPGSVGQTPLAPGRESSPSRLSTISALAGSQLSRVASVRHTRSHSTPMGFTGDNRGKHKRQQQQQQRNTAGLFSSIFTPSAKQSPPERQDPPPSSLRAVASSASALASAMGTPKSGNKGFFNFAFGSSNQPNLSPSSRSSTRALSPDRDTCLVDEFAGISFRALLQPYTLEPLGTPETPEEALKRTPEGRLGELCETAGDLLERVYKAYKARTDALSDVMDQQAEDQDLLEESKTKTAGYKLQLERLTTEERLAREEQQLRLAAYEKRIRDLEDELCREKAKVQDLEQRAAVANRKKRTSAASDSGFESDADSLFSIRERDRMASPVDSFLDTGIDETSSTTSTSTVTTSTASAAPLKCDSCAKLLHPISSASSTASMRNPTPLSQAWAPSNNSNNNNPTSKWGFAAALRGNRQTGVWGGSDAEIVRQENRMLRARVGELEVVVDDVLEVVAGRGVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEN5</accession>
    <name>D5GEN5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00001314001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430180">
        <property type="protein sequence ID" value="CAZ82978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838787.1">
        <property type="nucleotide sequence ID" value="XM_002838741.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEN5"/>
    <dbReference type="GeneID" id="9184889"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001314001"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009001_1_0_1"/>
    <dbReference type="InParanoid" id="D5GEN5"/>
    <dbReference type="OMA" id="GTVMAWA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051059">
        <property type="entry name" value="VerF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40626">
        <property type="entry name" value="MIP31509P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40626:SF11">
        <property type="entry name" value="ZINC FINGER PROTEIN YPR022C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="40"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="56"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="308"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="91"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="120"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="154"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ82978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="856" mass="93456" checksum="299D2E96DECE3D47" modified="2010-06-15" version="1">MRRTGPRVAKERKCNYCGKLFSKTEHLERHERSHTKDRPFQCQTCGKKYTRNDTLLRHSKSHNGGGGSGLHSSVNLTTGDIEGAKGEEPSPVSTSATTANFYSPSISPNFHENTGSYDGHSGGGAGDHGGGGTGNFDSPGGLLSDPARRHDPMSMQSVDPTLEISPQNIPQGTLPGLHTSPSFVDPSLDDASPHHPNSTPFVTDADGMQINLVSTVNSGFGSGDQWAQALFAEPSWLIGQGFDFGALNASIIPGFSDVNANIYVDDDTVSSVLPPPTITASLQENQSQIAVEYKPPRTNVAKLWFTSMGPSEENDEPSRPGSASLMSPDREMVDAGDENSGVFDEQYRRRLSNQMKPKWAEEPLPSVDFLNLCVQMYFTKCSILLPILHRATFKPTPENSFLLLTICTLGSLFIGSASARKQGERIFERVNKVILASWEKRLVRGAGEELALIQSSLIGQTYGLLSGSPSHLAIISAFHGTVISWARRAGMFQRNKTTPSPSGLSGEALEKVWHQWVKVEEAKRTVLGLYIHDVELASMFHHEPALRHHANILPLACSTELFEAPTASTWAAHYLAQDPNAVASAPIKTFKDFSDRIFPSWPVASPCPMTVSVPPVVTADYTCMYTLYISLLSINAAACDAQSLGNLRSPETFSSLSAALMQWHRYYQNALKLSPNGPQPCTFSLMALWHAVFLYLLVDINRLELAIGRDVNNPPSPAQEEVAYAVKWASTLSAKRAVIHGTLLQKQLANTPLDAEPAIHVPRALFHTGVVWYCWTRFAPQVDDGRNIHGEWDEMEMLGVDTDKLLGAVGSGRGVIGKANLCALTDMLRRAGHWGIARRFARILGELVFESGGNEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GET1</accession>
    <name>D5GET1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00001357001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430188">
        <property type="protein sequence ID" value="CAZ83024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838833.1">
        <property type="nucleotide sequence ID" value="XM_002838787.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GET1"/>
    <dbReference type="GeneID" id="9184938"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001357001"/>
    <dbReference type="HOGENOM" id="CLU_2470708_0_0_1"/>
    <dbReference type="InParanoid" id="D5GET1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="88" mass="9855" checksum="3869104975B185DD" modified="2010-06-15" version="1">MGTRDETCRASNYMREEIARLSVSPIYQSILSAAIILSPPTPVLCVISCHLQPTRQRQHTFSGPRTQHDTSSRLHYIDLTSNQPVSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEU5</accession>
    <name>D5GEU5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase</fullName>
            <ecNumber evidence="3 14">2.4.1.258</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Dol-P-Man-dependent alpha(1-3)-mannosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">GSTUM_00001398001</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11 14">Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. In the lumen of the endoplasmic reticulum, adds the first dolichyl beta-D-mannosyl phosphate derived mannose in an alpha-1,3 linkage to Man(5)GlcNAc(2)-PP-dolichol to produce Man(6)GlcNAc(2)-PP-dolichol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate = an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29527"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="Rhea" id="RHEA-COMP:19516"/>
            <dbReference type="Rhea" id="RHEA-COMP:19517"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:132515"/>
            <dbReference type="ChEBI" id="CHEBI:132516"/>
            <dbReference type="EC" id="2.4.1.258"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12 14">
            <dbReference type="Rhea" id="RHEA:29528"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Endoplasmic reticulum membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the glycosyltransferase ALG3 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.258" evidence="3 14"/>
    <dbReference type="EMBL" id="FN430194">
        <property type="protein sequence ID" value="CAZ83038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838847.1">
        <property type="nucleotide sequence ID" value="XM_002838801.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEU5"/>
    <dbReference type="FunCoup" id="D5GEU5">
        <property type="interactions" value="620"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GEU5"/>
    <dbReference type="GeneID" id="9184950"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001398001"/>
    <dbReference type="eggNOG" id="KOG2762">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035382_3_0_1"/>
    <dbReference type="InParanoid" id="D5GEU5"/>
    <dbReference type="OMA" id="KERTYCH"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052925">
        <property type="term" value="F:dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018279">
        <property type="term" value="P:protein N-linked glycosylation via asparagine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007873">
        <property type="entry name" value="Glycosyltransferase_ALG3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12646:SF0">
        <property type="entry name" value="DOL-P-MAN:MAN(5)GLCNAC(2)-PP-DOL ALPHA-1,3-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12646">
        <property type="entry name" value="NOT56 - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05208">
        <property type="entry name" value="ALG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 14" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="141"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="184"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="259"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00093457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU364047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAZ83038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="359" mass="40990" checksum="1486D0E48C535141" modified="2010-06-15" version="1">MDLLRTTIHSTFNSKSRFPLWLLPPLLLIDAVFCTTIILKVPYTEIDWKAYMEQVSQYRAGERDYVMIKGRTGPLVYPAGHVYIYNYLYGVTRGGVDVGLAQWIFMGVYLVTVGVVGLCYREARAPPYLLPFLILSKRLHSIYMLRLFNDPFSILLLFLAVFVWQKKLWTLGSLAYSLSVGVKMNTLLALPAVGGLYLLALGRDKALRQAGIMLQLQLLLATPFLTEFPMSYLNRAFEFTRQFFFQWTVNWRFVGEEVFLSKGFSLGLLGLHACLLLFFLVTRWSKPSDRSFPTRNPQVCPHHRSWCERNRHALRSQPALPVLLMACLGYPLPSLAQWVWAWVGYGALGCTGVGVERIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEV9</accession>
    <name>D5GEV9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="13">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00001413001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the ABC transporter superfamily. ABCB family. Heavy Metal importer (TC 3.A.1.210) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430196">
        <property type="protein sequence ID" value="CAZ83052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838861.1">
        <property type="nucleotide sequence ID" value="XM_002838815.1"/>
    </dbReference>
    <dbReference type="GeneID" id="9188592"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001413001"/>
    <dbReference type="eggNOG" id="KOG0056">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_257820_0_0_1"/>
    <dbReference type="InParanoid" id="D5GEV9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18583">
        <property type="entry name" value="ABC_6TM_HMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000287">
        <property type="entry name" value="Multidrug ABC transporter ATP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221:SF651">
        <property type="entry name" value="HEAVY METAL TOLERANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="152"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="373"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="401"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="480"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="12">
        <location>
            <begin position="262"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="580"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="949"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1076"/>
            <end position="1349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="981"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1076"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="1135"/>
            <end position="1147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1173"/>
            <end position="1185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1264"/>
            <end position="1275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1321"/>
            <end position="1331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ83052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1349" mass="148446" checksum="97A5EA727413892E" modified="2010-06-15" version="1">MSDTPEAFLKAYRAIHYVYPPLLLIFFVSLQTITCCTLQNLKPSKGVIRRNLMLFLQTAAIATYVGQTSVFLKASSVDGFVVANDLVLYVLSSVVIWGVIELCLVDNNSPVWYPYYGSWILSLIAELCLLALPNDDPGQKSGFRKAKISVEIIRVFFLFALPASYWGFQRSDNQKSFCEDEESAGLLTASNAVNETSNGTSYGATGGGNNNTPENAANTEANARMMKKIEESGNWWVYAKSFSILWKYLWPSEDRRLQASMVMVAFCLLAERGLNALVPHQLGVVTNSLSIGNGRAIPWLQVSIYVIFRWLDSGSGIPALHRYLWIPVDQYASRSITTAAYNHVMSLSHDFHTNKKSVEIYSSVRQGRSVNGFIESVLFAVLPMLADLCIAFAYFYYMFNAYMALIVAVVSIVYLYATAKLGATKNQIRRDYNTARIEEANVMCETMSSWTTVSYFNRIEYEQDRYWGAIKNYQKAELNYVLGITFLGVSQSLIFTVGLLGASFLAVYEVSRGNKPVGSFVTLLSYWAQLGSPLAFFANFYRKIQTQMLDAERLLELFQRKPSVVDKPGAQEFQLKSGEIEFNDVCFAYDPRKPAINNMSFRVPGGSTVALVGETGGGKTTCLKLLFRFFDVNSGSITIDDQDIRDVTLWSLRENMGVVPQDPQLFNDTLMMNLKYANFDATDEQVYAACRAAAIHNKILGFPDGYLTKVGEHGVKLSGGELQRVIAIARAILKNPKIVLLDEATSMVDMETERSIQRAFRELASNRTMFVIAHRLSTIMNANLIIVIKDGEIVEKGSHEELISAGGKYFQLWSKQLKNEQPKSSPGRPTELNYVNDLSPISREQELKKVSGELGTMSHQNPNLDNHPKPKTEGANLAANDFYIPILVPENGEGLSNSQSGLDKDNISKSLSNRQNFVAFAAELPGSDIQGEARVSSTHGILRNFGSVKNPEKQHMQLPRQDTPRTPSSLKPDAQEFIPNEPTSNGYAHQSISSDGAPVGNSTGCPLVSRESKNEVMMNALTDVIPFSDQSGNGEAGNGGDVHDQNGVGEDRIQALQEKIGVVLKFSEAKVKEMEERRASQMLPVKEKGPGTSEAPKSCIAMPNGNDGGNQCAVDTENSPMSPTGYRGGLLPEPKKKRRRNRRRSNKSKSTEESDSIANGGSHEKAQGAPLSESPSLPSMKNTSLHHCVGEEFPPALGGNSSIDKKQKRRCLDGPKTSNPKSEVERAVTSLSTLGRMISGEGLPGKVRGKNGISSAGESNLRGALKEDLRPKSGDGEYIVSPPNPVEHPTPISSVANRNTLREQNRRRSWGKSTALKGSWRRSDRDGRDEGVDATQGAGLTDGASAHSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEY6</accession>
    <name>D5GEY6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00006659001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430197">
        <property type="protein sequence ID" value="CAZ83079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838888.1">
        <property type="nucleotide sequence ID" value="XM_002838842.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEY6"/>
    <dbReference type="STRING" id="656061.D5GEY6"/>
    <dbReference type="GeneID" id="9188617"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006659001"/>
    <dbReference type="eggNOG" id="ENOG502RR25">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047019_0_1_1"/>
    <dbReference type="InParanoid" id="D5GEY6"/>
    <dbReference type="OMA" id="PRTHINF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019339">
        <property type="entry name" value="CIR_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039875">
        <property type="entry name" value="LENG1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22093">
        <property type="entry name" value="LEUKOCYTE RECEPTOR CLUSTER LRC MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22093:SF0">
        <property type="entry name" value="LEUKOCYTE RECEPTOR CLUSTER MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01083">
        <property type="entry name" value="Cir_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBF1-interacting co-repressor CIR N-terminal" evidence="2">
        <location>
            <begin position="10"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="292"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="322"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="361"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ83079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="370" mass="42116" checksum="0812D98524AD4117" modified="2010-06-15" version="1">MPLHLLHHKSYHVYSAENIARVRRDEVDAAAREAAEDARMQAADAEARMKLLRERAREGGSAVAAERGPERGALEQTARHIETQDHRPRQPLEGGILGADGHINLFPAPTAPQGGNPEREREKREEKERAEGAMGKAVGERRPWYSTVDLVSGRQREKEKDGRKSEWEGKRDDRRKEAGDPLGVIRRGVREVKEARRDREEWKRRREREVGGAEARGWQDGRDEKAERIERADRGGRDSGRKRRRRSFSRSGGGSPNRHDRSHKRHSHSHSRSVSHSQSRRHRHHRDDPGRHSHHHHSRSHHRGSGSDSGSRSISPNPTLESLRKEKLQRESAERQKASLLLAKAQADSEPGWKPVPGGRYSSQFGNRPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFA1</accession>
    <name>D5GFA1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00006796001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430208">
        <property type="protein sequence ID" value="CAZ83194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839003.1">
        <property type="nucleotide sequence ID" value="XM_002838957.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFA1"/>
    <dbReference type="GeneID" id="9188050"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006796001"/>
    <dbReference type="HOGENOM" id="CLU_2851348_0_0_1"/>
    <dbReference type="InParanoid" id="D5GFA1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072067" evidence="1">
        <location>
            <begin position="30"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="65" mass="7618" checksum="EC95EC3E8F7ADF88" modified="2010-06-15" version="1">MRYLFVTSENTNRIHALISWLLHILLGQASEEVYLISNAPSRYCDTDFFAKSSVFIENHGKHRYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFZ7</accession>
    <name>D5GFZ7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00001960001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430230">
        <property type="protein sequence ID" value="CAZ83440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839249.1">
        <property type="nucleotide sequence ID" value="XM_002839203.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFZ7"/>
    <dbReference type="STRING" id="656061.D5GFZ7"/>
    <dbReference type="GeneID" id="9181785"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001960001"/>
    <dbReference type="eggNOG" id="KOG0101">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009958_6_1_1"/>
    <dbReference type="InParanoid" id="D5GFZ7"/>
    <dbReference type="OMA" id="CGNQRIM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd10170">
        <property type="entry name" value="ASKHA_NBD_HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14187">
        <property type="entry name" value="ALPHA KINASE/ELONGATION FACTOR 2 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14187:SF82">
        <property type="entry name" value="FAMILY CHAPERONE, PUTATIVE (AFU_ORTHOLOGUE AFUA_7G08575)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="81"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="649" mass="72306" checksum="DAE64BA26018ACAA" modified="2010-06-15" version="1">MATDRSGTHGLSVVFRGRAEPVPSSPETERIIVGIDFGTTHSAIAWAQTSSPEEYEVITQWPGQSGKDWGKVPSDISYTTNRPASNPAPGSAPGSAPSPSPTPSVNSESNIPRPESTSSEAAALGFKWGFEVPPTDPQKLSWIKILLDPSQPRPNFVNPKKTTLPRGKKIVDIVTDYLGALKSYTVQTLERRLGKEIVQRSKVEYVLTVPAVWSDKARQMTMEAAIRAGLGVGSEDGGAGLLKLITEPEGAAEYALRSIQPNSMKVGDCFVVCDAGGGTVDLISYRISSLVPLQLNEVAIGTGALCGAVYLDRRFEDFVSKRIGKDTFNAMDHRARFQMNQFWETYVKREFVDDEGVQRDEAEEEENESKLFWVPVMGVPDDEYKGIRGGFLKLSRREVKEIFEPIVAQVLELISKQVIEVLKSGKGPIAAILLVGGFGSSEYLYQRINRWAADKIDVMQPRNAWTAVVRGAIIRGLQGDIAAVQSRLSRRHYGVVHDSRYDESKHDESERVWHPLRCEWVVKNRIQWYIRRGDVTTERRKISFPFYRTVAESDLVGDTHQLKTELWVCDEEAAPDKRVKGDDSVRKLCVMTSEPVPVSRFTTHTNEKAETFYRVDYSLDMTVTSGVVVYEMSLEGEKASVGRVTTKYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5GG83</accession>
    <name>D5GG83_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00001997001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430246">
        <property type="protein sequence ID" value="CAZ83526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839335.1">
        <property type="nucleotide sequence ID" value="XM_002839289.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GG83"/>
    <dbReference type="STRING" id="656061.D5GG83"/>
    <dbReference type="GeneID" id="9184593"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001997001"/>
    <dbReference type="eggNOG" id="KOG0725">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_1_1"/>
    <dbReference type="InParanoid" id="D5GG83"/>
    <dbReference type="OMA" id="LFGVQCD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000398">
        <property type="entry name" value="Probable 2-deoxy-D-gluconate 3-dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760:SF5">
        <property type="entry name" value="2-DEHYDRO-3-DEOXY-D-GLUCONATE 5-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASES/REDUCTASES FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ83526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="259" mass="27400" checksum="DC297348C3754B90" modified="2010-06-15" version="1">MSSMFDLSGKTALVTGGTRGIGQTMALALAEAGADIILVQRDSKNISTKAQIAAMGRACIIVNADLSSDAEVSTLIPGLIADPSSLPNQRIDILLNCAGIQRRHPSHQFPTKDFQAVIQVNLATVFTLCRDIGAYFIGNQIHGRIINVASVLSFQGGLTVPAYAASKGAVLQLTKALSNEWAGKGIGVNAIAPGYIATDMNEALIKDEARSRQIMERIPAGRWGRPEDFKGPVVFLASEKASAYVSGECLVVDGGWMGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGB6</accession>
    <name>D5GGB6_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NADH-ubiquinone oxidoreductase chain 4L</fullName>
            <ecNumber evidence="4">7.1.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">NADH dehydrogenase subunit 4L</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">GSTUM_00002005001</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29091"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="7.1.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I subunit 4L family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.2" evidence="4"/>
    <dbReference type="EMBL" id="FN430254">
        <property type="protein sequence ID" value="CAZ83559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839368.1">
        <property type="nucleotide sequence ID" value="XM_002839322.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGB6"/>
    <dbReference type="STRING" id="656061.D5GGB6"/>
    <dbReference type="GeneID" id="9183162"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002005001"/>
    <dbReference type="eggNOG" id="KOG4669">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1416123_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGB6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030964">
        <property type="term" value="C:NADH dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.3510:FF:000004">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase chain 4L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.28.10">
        <property type="entry name" value="Homing endonucleases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01456">
        <property type="entry name" value="NDH1_NuoK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027434">
        <property type="entry name" value="Homing_endonucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004860">
        <property type="entry name" value="LAGLIDADG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001133">
        <property type="entry name" value="NADH_UbQ_OxRdtase_chain4L/K"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039428">
        <property type="entry name" value="NUOK/Mnh_C1-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004320">
        <property type="entry name" value="PRK05715.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11434:SF16">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 4L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11434">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE SUBUNIT ND4L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00961">
        <property type="entry name" value="LAGLIDADG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00420">
        <property type="entry name" value="Oxidored_q2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55608">
        <property type="entry name" value="Homing endonucleases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="8 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="55"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="Homing endonuclease LAGLIDADG" evidence="15">
        <location>
            <begin position="98"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAZ83559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="192" mass="21625" checksum="84B49679E3EA931B" modified="2010-06-15" version="1">MSISLLLFLIGTLGFVLNRKNIILMLISIEIMLLAITFLILVSSLSFDDILGQTYAIYIIAIAGAESAIGLGILVAFYRLREISLPTPTYSLLPLKKLVQNQLHETDRPLLLLIPKFFGNIGAVSTKNTRSTVEFRVSTLSHLINVIIPHFEKYSLITNKHSDFFFKQIVLLMSENQHRNLDGLQLILNNRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHF1</accession>
    <name>D5GHF1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="12">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00007904001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430318">
        <property type="protein sequence ID" value="CAZ83944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839753.1">
        <property type="nucleotide sequence ID" value="XM_002839707.1"/>
    </dbReference>
    <dbReference type="GeneID" id="9183619"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007904001"/>
    <dbReference type="eggNOG" id="KOG0065">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_35_0_1"/>
    <dbReference type="InParanoid" id="D5GHF1"/>
    <dbReference type="OMA" id="QYWSDQS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03233">
        <property type="entry name" value="ABCG_PDR_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03232">
        <property type="entry name" value="ABCG_PDR_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001010">
        <property type="entry name" value="ABC multidrug transporter (Eurofung)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000054">
        <property type="entry name" value="ABC multidrug transporter atrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029481">
        <property type="entry name" value="ABC_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034001">
        <property type="entry name" value="ABCG_PDR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034003">
        <property type="entry name" value="ABCG_PDR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010929">
        <property type="entry name" value="PDR_CDR_ABC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19241">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14510">
        <property type="entry name" value="ABC_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06422">
        <property type="entry name" value="PDR_CDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="523"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="556"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="598"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="632"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="665"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="693"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="156"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="850"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="820"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="25"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="58"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ83944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1405" mass="156593" checksum="3875D25E419AB749" modified="2010-06-15" version="1">MSEKLVSTAPSGDAIMSDTEGVRQDPSTSNPRGNSNIHTGVDVAAAEAEFQELSRQLTGLSKTASRQQSRRMSRTISRTQSRILGGVGIKSGGDPEKAGGEEEEEFNLEDHLRGNQSADRAAGIRPKHIGVIWQDLTVSGIGGVRNFISTFPDAVVGFFNIFATASSILGLNKKGREFDILQGFRGVVKPGEMVLVLGRPGSGCTTFLKVIANQRFGYTKVGGEVLYGKFPADTFAQRYRGEAVYNEEDDVHHPTLTVGQTLGFALDTKTPGKRPAGMSKQEFKDSVVTLLLKMFNIEHTINTIVGNPFVRGVSGGERKRVSIAEMMITKATVCCWDNSTRGLDASTALDYAKSLRIMTNIYKTSTFVSLYQASENIYAQFDKVLVIDEGREVFFGPTSEARAYFEGLGFLPKPRQTTPDYLTGCTDEFEREFASGRDETNVPSSPVALEEAFHKSPHFVRLNQEMEAYRETLGEEKFENFEAAVREEKRRGAPKTSVYTIPFFQQIWTLMQRQFLLKWQDKFSLVVSWITSITIAIVLGTVYLDLPKTSAGAFTRGGLLFIAMLFNAFQTFGELGATMLGRPIVNKHKAYTFHRPSALWIAQVVVDVVFAAAQTMVFSIIVYFMSGLVRDAGAFFTFWLMIITGYLAMTVFFRTIGCICPDFDYAMKFAAVIITFFVLTSGYLIPYPAQGKWVSWIFYVNGLGLGFSSLMMNEFKRLTLTCTSDSLVPSGPGYENLRYQTCTLLGSSPGSSEIDGGDYINLSFQYDPKDLWRNFGITIVLIAAFLASNALLGEYLSYGAGGKTITYYAKENAERKALNQKLTQKRESRRQKQAAEEEESTLKIESRSILTWEKLVYEVPTPSGQLRLLNEIYGYVRPGELTALMGASGAGKTTLLDVLASRKNIGVITGDVLVDGKPKGTAFQRGTSYAEQLDTHEPTQTVREALRFSAYLRQPYEVSRAEKDTYVEEVIALLEMENIADAIIGDPTNGLAVEERKRVTIGVELAAKPQLLLFLDEPTSGLDSQSAFNIVRFLKKLAKAGQAILCTIHQPNSSLFENFDRLLLLQHGGECVYFGDIGKDAQALLGYFAKHGAYCPSDANPAEWMLDAIGAGLAPRIGDRDWGDVWRSSEELASIKAMISRMKDERIAEVGSVPPVDEKEYATPVMFQVKEVMKRMNLSFWRTPNYGFTRLFNHIAIALLTGLVFLDLDESRSSLQYRVFIIFQSTVLPSLIIAQVEPKYDMSRPKFYREQSSKMYXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXHSQTQHSQVLARVPLPTRPFHTPNRRNGRDGAPRRFGNLCLQRVQHLPHTGWGDVWWVCRKVHGGRARVYPGFERYGIVRLLCFLGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHH2</accession>
    <name>D5GHH2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00007875001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430318">
        <property type="protein sequence ID" value="CAZ83965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839774.1">
        <property type="nucleotide sequence ID" value="XM_002839728.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHH2"/>
    <dbReference type="FunCoup" id="D5GHH2">
        <property type="interactions" value="157"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GHH2"/>
    <dbReference type="GeneID" id="9183610"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007875001"/>
    <dbReference type="eggNOG" id="KOG0872">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047036_3_0_1"/>
    <dbReference type="InParanoid" id="D5GHH2"/>
    <dbReference type="OMA" id="GPGLWYN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000248">
        <property type="term" value="F:C-5 sterol desaturase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050307">
        <property type="entry name" value="Sterol_Desaturase_Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11863">
        <property type="entry name" value="STEROL DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="77"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="125"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="163"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="236"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid hydroxylase" evidence="6">
        <location>
            <begin position="168"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ83965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="347" mass="41073" checksum="C95B9BA143A983E2" modified="2010-06-15" version="1">MDYILGVADNLFFDAIYATLLPAGSLASWAGANDTLTEEYMNPPIIVDDPISHFFGLRPSHRAYESLFLRGDWQRQAFSLFLITWIFGLVVYLVFASLSYFLIFDKDTFKHPKFLKNQIRQEMAQTIRSLPIMAIFTTPFFLAEVRGYSKLYADVELHGKWYLWFQFPLFLMFTDFWIYLIHRGLHHKSIYKTIHKPHHKWIMPTPFASHAFHPIDGFSQSIPYHVFPFIFPLHKFAYIGLFIFINVWTIMIHDGEYVANSPIVNGAACHTMHHLYFNYNYGQYTTLWDRLLGSYRKPNMELFYRETKMGKDEWEKQVKEMEKVVKEVEGDDTFREYAVDVSHKKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHL5</accession>
    <name>D5GHL5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="12">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00007976001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="FN430319">
        <property type="protein sequence ID" value="CAZ84008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839817.1">
        <property type="nucleotide sequence ID" value="XM_002839771.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHL5"/>
    <dbReference type="FunCoup" id="D5GHL5">
        <property type="interactions" value="38"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GHL5"/>
    <dbReference type="GeneID" id="9183662"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007976001"/>
    <dbReference type="eggNOG" id="ENOG502QRIP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021264_11_0_1"/>
    <dbReference type="InParanoid" id="D5GHL5"/>
    <dbReference type="OMA" id="HDSHQHT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10951">
        <property type="entry name" value="CE4_ClCDA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00035">
        <property type="entry name" value="ChtBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.10">
        <property type="entry name" value="Endochitinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.370">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001002">
        <property type="entry name" value="Chitin-bd_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036861">
        <property type="entry name" value="Endochitinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002509">
        <property type="entry name" value="NODB_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46471">
        <property type="entry name" value="CHITIN DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46471:SF2">
        <property type="entry name" value="CHITIN DEACETYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00187">
        <property type="entry name" value="Chitin_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00270">
        <property type="entry name" value="ChtBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57016">
        <property type="entry name" value="Plant lectins/antimicrobial peptides"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50941">
        <property type="entry name" value="CHIT_BIND_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51677">
        <property type="entry name" value="NODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="2 8" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="7" id="KW-0170">Cobalt</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072159" evidence="9">
        <location>
            <begin position="26"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-1" evidence="10">
        <location>
            <begin position="40"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="NodB homology" evidence="11">
        <location>
            <begin position="128"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="63"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ84008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="328" mass="34345" checksum="D0E2ED556B909EDB" modified="2010-06-15" version="1">MISPISSIGLLGLSTYFGALVGSTAIPPNNIFSRQSTTPDGTCGVQNSGNGRGYRCTTGSSACCSQWGWCGDGSEYCGSGCQPSYGSCGSTGGGNPPGGGGNPPTTGGRTKPGSVPYGTVISSCTAGGVVALTFDDGPYTYTSNLLDLLARYGAKATFFVNGQNWGAPITDPSKQDLLRRMVSDGHQIGSHTWSHPALSSLSTADMTTQMTSLESAIATAIGRYPTYMRPPYFDCNSNCLGVMDSLGYHVIFSNLDTLDWANQGNIQASKDIFSRNVASGSLVLAHDVHPDTVGTLAEHMIVESQNRGLRLVTLGDCLGDPAENWYRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHX0</accession>
    <name>D5GHX0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008156001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430321">
        <property type="protein sequence ID" value="CAZ84113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839922.1">
        <property type="nucleotide sequence ID" value="XM_002839876.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHX0"/>
    <dbReference type="GeneID" id="9184206"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008156001"/>
    <dbReference type="HOGENOM" id="CLU_2428670_0_0_1"/>
    <dbReference type="InParanoid" id="D5GHX0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="91" mass="9686" checksum="9B6EB137B250BC60" modified="2010-06-15" version="1">MNCRGGPKGTSSQAAPNGESGANRTPAPPPNPSQAGPLVEIQERLPQVLRIASPNASQQNLEFRALSDRVVEESGANQLWISRVLRERGNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GI52</accession>
    <name>D5GI52_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00008279001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TTC4 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430323">
        <property type="protein sequence ID" value="CAZ84195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840004.1">
        <property type="nucleotide sequence ID" value="XM_002839958.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GI52"/>
    <dbReference type="FunCoup" id="D5GI52">
        <property type="interactions" value="1188"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GI52"/>
    <dbReference type="GeneID" id="9181913"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008279001"/>
    <dbReference type="eggNOG" id="KOG0551">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040446_1_0_1"/>
    <dbReference type="InParanoid" id="D5GI52"/>
    <dbReference type="OMA" id="WRAAQCA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd21381">
        <property type="entry name" value="CTWD_TTC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044059">
        <property type="entry name" value="Csn1/TTC4_wheel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46035">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46035:SF1">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18972">
        <property type="entry name" value="Wheel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="Cns1/TTC4 wheel" evidence="4">
        <location>
            <begin position="274"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF18972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ84195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="394" mass="43369" checksum="ADD9A4717D73C693" modified="2010-06-15" version="1">MATVTELPTGVDNENLDMPRFVEEATARHAIPTPTQTTSGAALPPAISGVKNTTTEEFLKAMNKMPLFMTELDESNEDGTGENLELEALKALAYEGEPHEVAQNFRNQGNDCYRARAWRDAVEYYTKALALKCGVAEIEEACYVNRAAANLELKNLRKVIADTKSALQLNPRNTKAWYRSARALLALDKLPESLQCIDNGLSTSPTNQPLITLQEQALSRQSHLSALETVRRQRVQRQLAIEKTLAAALAARSITVTRTPHPPDMEDVQVHLEDPLDPTSELYFPAIFLYPLQGQSDFVKAFPVSSKVGMQLGIVLAKFPEWDHEKAYSPPGKVEVFVETRTGGLVRVGKKAALGSVLGNGGKVEVVDGLVKFLVVPKGEVGAFLEDWKRRMGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>D5GI73</accession>
    <name>D5GI73_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008314001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430323">
        <property type="protein sequence ID" value="CAZ84216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840025.1">
        <property type="nucleotide sequence ID" value="XM_002839979.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GI73"/>
    <dbReference type="GeneID" id="9181934"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008314001"/>
    <dbReference type="eggNOG" id="ENOG502SF70">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059402_2_1_1"/>
    <dbReference type="InParanoid" id="D5GI73"/>
    <dbReference type="OMA" id="QKTYESE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35587:SF4">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35587">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="42"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="80"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="133" mass="13926" checksum="0AED0C4BFA65853A" modified="2010-06-15" version="1">MASQGQTRASDLSETESEYDPAPRTQSRRRGRGRSRKNKKAGQLQQQQQKSGPLQGLGPGQQLSQTTGQIGEVAGGLSGSLGNTLGQTAGQVAGGGGGGGKKNDTLKLRLDLNIDIHIELRAKIHGDLELSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>D5GI80</accession>
    <name>D5GI80_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008324001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430324">
        <property type="protein sequence ID" value="CAZ84223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840032.1">
        <property type="nucleotide sequence ID" value="XM_002839986.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GI80"/>
    <dbReference type="GeneID" id="9181941"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008324001"/>
    <dbReference type="eggNOG" id="ENOG502RX75">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008809_0_0_1"/>
    <dbReference type="InParanoid" id="D5GI80"/>
    <dbReference type="OMA" id="GNDPFPM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35041:SF3">
        <property type="entry name" value="FORMYLMETHIONINE DEFORMYLASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35041">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="637" mass="70671" checksum="3DE10D0EAC1C9CA8" modified="2010-06-15" version="1">MEQYDPYPPQGDANERAPSPMRVERVISGAGSVASNASTGSRLRERIRRDMEWGSKLHWFLPSAMIGLFILGLLGALLHHWFYVSLHGKGAKDQLIMVRFGTAFAFFTKAALVGSIVLAYRQRIWYTLRTKPITVKGIDNLFALTEDPTGFYSKDGATRAKVATLMALATWVIPLAAVLSPGALTAQLSRVESTADCTVPAVNTSAQNDAVQDQPKYGVLGYDLVYFNNSDPPGHDQYFGGTGYLFTEATNFAFTLKKPRDISSPCKGYNCTWNAVFDAPWYECHEKSVSEARNAFDTMDWVDIESRHWAPRSQVTLVAVWDGEEYASQQPLWNGSDNMQGYFTGEPTIRVGYVVDTGIPVVEGSVDSDSGLWKTVLEPHWLSCDLFKASWNVTFNFTGTPSARVKTSNRRRLFEPPKMGPTHPNYRENALYYSFGKLVREKLRGGLGVRNGTSDTRRISSYNPLVNVTTRMAINDVKGGVEKLVIDLGLTLLTFPYLDIAVNTSVGCTKWRYENRFHYSPQGLWIGYTLSVIATLASVLVGMHSIYVNGITSDTLFSKILVTTRNPTLDKLVREHEGVCLGGGPFPEKLENTRLLFGIIDNGSHTAFGIVDETTVMLDPVGYRGLINPRWGHSTGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIE2</accession>
    <name>D5GIE2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="13">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00008446001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Transport of phosphate groups from the cytosol to the mitochondrial matrix.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430326">
        <property type="protein sequence ID" value="CAZ84285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840094.1">
        <property type="nucleotide sequence ID" value="XM_002840048.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIE2"/>
    <dbReference type="FunCoup" id="D5GIE2">
        <property type="interactions" value="741"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GIE2"/>
    <dbReference type="GeneID" id="9184643"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008446001"/>
    <dbReference type="eggNOG" id="KOG0767">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039456_2_1_1"/>
    <dbReference type="InParanoid" id="D5GIE2"/>
    <dbReference type="OMA" id="YKGAIWL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035434">
        <property type="term" value="P:copper ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990547">
        <property type="term" value="P:mitochondrial phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000076">
        <property type="entry name" value="Mitochondrial phosphate carrier protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000131">
        <property type="entry name" value="Mitochondrial phosphate carrier protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044677">
        <property type="entry name" value="SLC25A3/Pic2/Mir1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 (MITOCHONDRIAL CARRIER PHOSPHATE CARRIER), MEMBER 3, LIKE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671:SF10">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="81"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="178"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="279"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00054508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ84285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="379" mass="40861" checksum="F60F2A7DDB1528B7" modified="2010-06-15" version="1">MSGIATPTCANSSVVFPPTPFSVTVLQKKARPAQASAASLYPYSSISEEAKKLGKKAQAEFPYASEVTQGKAGKIDMYSPKFYAACTTGGILACGLTHTAVTPLDLVKCRRQVDSKLYRSNIQAWRTISRAEGIRGIFTGWSPTFFGYCAQGGLKYGGYEFFKKFYSDLAGPEKAAKYKTAIYLAGSASAEFIADVALCPLESVKVRTQTTIPPEFKGTTEGLKSVVAKEGFGGLYKGLYPLWGRQIPYTMMKFASFETVVEMIYDYLPKSKDQYGSGVQTTVSFAGGYIAGILCAIVSHPADVMVSKLNANRRPGEAFGAVMKRIYGEIGFGGLWNGLPVRIVMIGTLTGLQWLLYDSFKLAVGLPTTGGSALEKGEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIZ7</accession>
    <name>D5GIZ7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00008755001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840299.1">
        <property type="nucleotide sequence ID" value="XM_002840253.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIZ7"/>
    <dbReference type="FunCoup" id="D5GIZ7">
        <property type="interactions" value="353"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GIZ7"/>
    <dbReference type="GeneID" id="9181831"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008755001"/>
    <dbReference type="eggNOG" id="KOG3911">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022876_0_2_1"/>
    <dbReference type="InParanoid" id="D5GIZ7"/>
    <dbReference type="OMA" id="AMWFSDR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010301">
        <property type="entry name" value="RRP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13026">
        <property type="entry name" value="NNP-1 PROTEIN NOVEL NUCLEAR PROTEIN 1 NOP52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13026:SF0">
        <property type="entry name" value="RIBOSOMAL RNA PROCESSING 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05997">
        <property type="entry name" value="Nop52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="232"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="236"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ84490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="258" mass="30011" checksum="32A869274DFB1F94" modified="2010-06-15" version="1">MTETKLQKTLFVKQLAANDRPTRDRAVASLRTYLSSRRSFTKIEFLKLWKGLFYCMWLSDRPRTQQRLVLDLAGLLDSMETKNAGLFLEAFWNTIAREWNGVDVLRMDKFLLLVRGYLASSFRYLHARGWEKDLVGRFTNLLRETPLSPKDMKVPNGLRYHLIDIYVDELEKLKPEEMKIPFPIATLLSPFEELTLASPTKLVRTKSKELLCDGRLADWGYDKAIGAIKSRRKEVEEEDCGDDEEMGDEGEEWYGIDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJ52</accession>
    <name>D5GJ52_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00008841001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840354.1">
        <property type="nucleotide sequence ID" value="XM_002840308.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJ52"/>
    <dbReference type="GeneID" id="9181886"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008841001"/>
    <dbReference type="eggNOG" id="ENOG502RXM1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067132_1_0_1"/>
    <dbReference type="InParanoid" id="D5GJ52"/>
    <dbReference type="OMA" id="NKAADFG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022226">
        <property type="entry name" value="DUF3752"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046331">
        <property type="entry name" value="GPAM1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46370">
        <property type="entry name" value="GPALPP MOTIFS-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46370:SF1">
        <property type="entry name" value="GPALPP MOTIFS-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12572">
        <property type="entry name" value="DUF3752"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3752" evidence="2">
        <location>
            <begin position="122"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ84545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="277" mass="30566" checksum="47B1A9DB8E356E1C" modified="2010-06-15" version="1">MGGTVGPSIPPHILAKRKLDKEENAAATSKIGLSAPDDLDIKKRRTVGPAPPPLNEQPNSGSEDGDDETISDDDIGPALPPSSASAEEVELAARRRLAQFAETKFQNSDGGKAKRDEWMLVPPKSEDWTTKVDPTKLRNRKFQTGKGSKAPQKPGGDNTLWTESPEEKRKRLNDEVMGIKKPATQTQEKEGKHGVSAAEAEETARRIREYNEKNRSQSLYEEHKKLGPREKEDDPGARAFDREKDIAGGRRIGHAQKKEMLNRAAEFGSRFSSGSYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJ60</accession>
    <name>D5GJ60_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Golgi apparatus membrane protein TVP23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00008850001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Golgi membrane protein involved in vesicular trafficking.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Golgi apparatus membrane</location>
            <topology evidence="2 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the TVP23 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840362.1">
        <property type="nucleotide sequence ID" value="XM_002840316.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJ60"/>
    <dbReference type="FunCoup" id="D5GJ60">
        <property type="interactions" value="371"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJ60"/>
    <dbReference type="GeneID" id="9185921"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008850001"/>
    <dbReference type="eggNOG" id="KOG3195">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074845_1_1_1"/>
    <dbReference type="InParanoid" id="D5GJ60"/>
    <dbReference type="OMA" id="FEWMIVA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008564">
        <property type="entry name" value="TVP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13019">
        <property type="entry name" value="GOLGI APPARATUS MEMBRANE PROTEIN TVP23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13019:SF7">
        <property type="entry name" value="GOLGI APPARATUS MEMBRANE PROTEIN TVP23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05832">
        <property type="entry name" value="DUF846"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ84553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="192" mass="21551" checksum="81B30B9B2D1C41F1" modified="2010-06-15" version="1">MDSQQGALSWRLSAHPLTLGFFLAFRLASIFTYLFGLWFSSNFILIFIIVILLLAADFYYCKNIAGRRLVGLRWWNETNPETGEQTWVFESADETRQINATDSRFFWLTIYVVPLLWVFLAIIAIVRFEFIWLSLVVIAVILCATNGVAFSRADKFGNASSLAGSALNSSGGGAVRRFAGNMATRMFFSGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJB4</accession>
    <name>D5GJB4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00008922001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
            <location evidence="1">Centromere</location>
            <location evidence="1">Kinetochore</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mis12 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430330">
        <property type="protein sequence ID" value="CAZ84607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840416.1">
        <property type="nucleotide sequence ID" value="XM_002840370.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJB4"/>
    <dbReference type="GeneID" id="9185975"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008922001"/>
    <dbReference type="eggNOG" id="ENOG502S72R">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1788230_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJB4"/>
    <dbReference type="OMA" id="TWPPITL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000444">
        <property type="term" value="C:MIS12/MIND type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051382">
        <property type="term" value="P:kinetochore assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000070">
        <property type="term" value="P:mitotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008685">
        <property type="entry name" value="Centromere_Mis12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14527">
        <property type="entry name" value="PROTEIN MIS12 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14527:SF2">
        <property type="entry name" value="PROTEIN MIS12 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05859">
        <property type="entry name" value="Mis12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="9" id="KW-0137">Centromere</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ84607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="145" mass="16326" checksum="FDA5A9A856668567" modified="2010-06-15" version="1">MNPASTSLLTEHLRWTPLSLIDDIINTVNALLYQSVSAVETFLLSSPPGLLGFAPPPGTIPDTDGDGNVIYSEKEEEEINKGIHQLETLLENGVDKRFDAFELYVLRNILVVPQDLVGWVRLAHHKVSLQYCSPFRTLFYIPQKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJB6</accession>
    <name>D5GJB6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00008926001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 15 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430330">
        <property type="protein sequence ID" value="CAZ84609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840418.1">
        <property type="nucleotide sequence ID" value="XM_002840372.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJB6"/>
    <dbReference type="STRING" id="656061.D5GJB6"/>
    <dbReference type="GeneID" id="9185977"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008926001"/>
    <dbReference type="eggNOG" id="KOG4472">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024327_4_1_1"/>
    <dbReference type="InParanoid" id="D5GJB6"/>
    <dbReference type="OMA" id="CPSAYYM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002685">
        <property type="entry name" value="Glyco_trans_15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121">
        <property type="entry name" value="ALPHA-1,2 MANNOSYLTRANSFERASE KTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121:SF6">
        <property type="entry name" value="ALPHA-1,2 MANNOSYLTRANSFERASE KTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01793">
        <property type="entry name" value="Glyco_transf_15"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018153">
        <property type="entry name" value="Glyco_trans_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR018153-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ84609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="390" mass="45687" checksum="DF1F0C054BD82C5E" modified="2010-06-15" version="1">MDPKLLWQRADTGSRISARFFTRRLSLRVLALLSICVVLYNVVYSTELRRRLEMNVQAPRHTGQPQHGTDTAELEDGPPSATCFGAELGHNGTEKATLVMLVRNSELHGALSSIRMVEDRFNRRFHYPWTFLNDKPFTDDFIKYTSGLASGPVEYAVIPEDDWSIPDHVNTTIMRAEMLRMAKQEIIYGGSLSYRHMCRFNSGFFYNQEVMRKYDWYWRVEPGIELYCDINYDPFTFMRENKKIYGFVISMYEFRDTITTLWDRTRQFLNHGDYNLCHFWSNFEIASLEFYRSEAYSRYFEFLDSTGGFFYERWGDAPVHSIGVGLFAPKDSIHHFADFGYSHPPCARCPQDDESHTSGRCVCSRNKNFDSDSYSCLPRWWKVNRGTGWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJB9</accession>
    <name>D5GJB9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00008931001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430330">
        <property type="protein sequence ID" value="CAZ84612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840421.1">
        <property type="nucleotide sequence ID" value="XM_002840375.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJB9"/>
    <dbReference type="GeneID" id="9185980"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008931001"/>
    <dbReference type="eggNOG" id="KOG2254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123444_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJB9"/>
    <dbReference type="OMA" id="SEYHCEN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018909">
        <property type="entry name" value="Eng1_septum"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10645">
        <property type="entry name" value="Carb_bind"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072392" evidence="1">
        <location>
            <begin position="20"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Endo-1,3(4)-beta-glucanase 1 carbohydrate binding" evidence="2">
        <location>
            <begin position="33"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="Endo-1,3(4)-beta-glucanase 1 carbohydrate binding" evidence="2">
        <location>
            <begin position="87"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ84612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="134" mass="14696" checksum="52B70531F8D5BC0A" modified="2010-06-15" version="1">MQFTTYVLATFTLISAALASPVNNLVERQLQYCGTQPYYPQDYTCYPANNNLLCPISNGVIFQPCGQACFDPANYGCVNGQLVPVGNCNGQAYDKNSYVCVSNHLCPKTHPNLCGEACYSLSQYRCNNGQLVQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJK3</accession>
    <name>D5GJK3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00009051001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430332">
        <property type="protein sequence ID" value="CAZ84696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840505.1">
        <property type="nucleotide sequence ID" value="XM_002840459.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJK3"/>
    <dbReference type="GeneID" id="9186793"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009051001"/>
    <dbReference type="HOGENOM" id="CLU_2607760_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJK3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ84696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="79" mass="8874" checksum="67ACDA4FCAB7CB8F" modified="2010-06-15" version="1">MEALLTALRQRHAELRSQNDRNVRLGVGMENLLRPVEDGIRIEREREAAELASKKMDFGLRAEAGKGDEDVGTVGRRSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJU2</accession>
    <name>D5GJU2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00009195001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430333">
        <property type="protein sequence ID" value="CAZ84785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840594.1">
        <property type="nucleotide sequence ID" value="XM_002840548.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJU2"/>
    <dbReference type="GeneID" id="9186821"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009195001"/>
    <dbReference type="HOGENOM" id="CLU_1327242_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJU2"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="76"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="167"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ84785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="207" mass="22589" checksum="59A3EDB8E20C86A8" modified="2010-06-15" version="1">MGTSILPSGDPRWNRTPIRPGFFVFILITILNAGICIAETAKRSHVMGVLVLTVSLVTTVHLAIDMYLFFVKGRLHGISVFLLFFAEFCLWMVVAVSEAMDLGFLSKPKGWFNTDGPFPECGFGDNMVNGGICELMRARFFISLAILIFSFAFTLFSALGPAPETRPISSLMESGDGEGGEGAQRCSDQEQVQPPNMDKAQPVVEVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5GK29</accession>
    <name>D5GK29_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00009336001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ORC5 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430335">
        <property type="protein sequence ID" value="CAZ84872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840681.1">
        <property type="nucleotide sequence ID" value="XM_002840635.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GK29"/>
    <dbReference type="FunCoup" id="D5GK29">
        <property type="interactions" value="795"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GK29"/>
    <dbReference type="GeneID" id="9186909"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009336001"/>
    <dbReference type="eggNOG" id="KOG2543">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028223_2_0_1"/>
    <dbReference type="InParanoid" id="D5GK29"/>
    <dbReference type="OMA" id="QLRRWHG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005664">
        <property type="term" value="C:nuclear origin of replication recognition complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041664">
        <property type="entry name" value="AAA_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020796">
        <property type="entry name" value="ORC5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047088">
        <property type="entry name" value="ORC5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048866">
        <property type="entry name" value="ORC5_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12705">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12705:SF0">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13191">
        <property type="entry name" value="AAA_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14630">
        <property type="entry name" value="ORC5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21639">
        <property type="entry name" value="ORC5_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Orc1-like AAA ATPase" evidence="7">
        <location>
            <begin position="17"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="ORC5 lid" evidence="9">
        <location>
            <begin position="199"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Origin recognition complex subunit 5 C-terminal" evidence="8">
        <location>
            <begin position="283"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF14630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ84872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="424" mass="47310" checksum="0288C8B45F8780F1" modified="2010-06-15" version="1">MTDEMLPTELLTTMNEQFPCRAPQIRRLAALVGTDETPSPPAIVLHGLEATGKTTILRTFMDSVNASYTAGVTENVGGGDAGRAGDVCALGVRLSGVLSGVERKHFLVLDRVDRTREGSSVLFAALGRLGEMIPILTVIFILSVPKPRLLSSAEPPHIHFAPYTKEESIKILSKYVRRIPFQDIEEYTQKDAKEELYVWQKFCGTVWDSLAKGTARGVVQFRAIVDEMWEPFVKPIAEGEYGTRNYSSLYLLHKDMFRRETNVIDVVVPVGAGEKAVTKIHDLPYYSKFLLCAAYLASYNPARQDAVFFMKNNDFGRKKRRGGTTGSTKRTAKNRKIHRRLLGPQAFPLERLQAIFAAILPHRMSSSADIQTQIATLTSLRLLTKASATDVLEAGAKWRVNAGWDYIRHVARSVKFDIEDFVAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>D5GK56</accession>
    <name>D5GK56_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00009371001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430335">
        <property type="protein sequence ID" value="CAZ84899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840708.1">
        <property type="nucleotide sequence ID" value="XM_002840662.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GK56"/>
    <dbReference type="STRING" id="656061.D5GK56"/>
    <dbReference type="GeneID" id="9186936"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009371001"/>
    <dbReference type="eggNOG" id="ENOG502S4YJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1120805_0_0_1"/>
    <dbReference type="InParanoid" id="D5GK56"/>
    <dbReference type="OMA" id="TANAHTY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase zinc-binding" evidence="2">
        <location>
            <begin position="102"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="172"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="174"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ84899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="248" mass="27360" checksum="766A4CCED299C466" modified="2010-06-15" version="1">MGNAFEDTPTSLEGFPTQAEFENLLNEYILSLSPKKRDKALIPQKRYDNILAVLRDPKCTTIESAQFRFWAKKMFRLVPDDDNVGVVCHEGKPVAVREQLYNVLTSSHGQAQHGGRDKTSAQVRKYFSWVPKELIARFVRNCPSCTLRRSSPLEYAVANNARQAIDATPTYITSSASSSQRSSVSSMSSPMSTVPPNHQVVAQLALVSSAPPSSLVPATANAHTYSFSYPEMPLDPRMYATGPSDLSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>D5GK59</accession>
    <name>D5GK59_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00009379001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430336">
        <property type="protein sequence ID" value="CAZ84902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840711.1">
        <property type="nucleotide sequence ID" value="XM_002840665.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GK59"/>
    <dbReference type="GeneID" id="9186940"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009379001"/>
    <dbReference type="eggNOG" id="ENOG502S005">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032571_1_0_1"/>
    <dbReference type="InParanoid" id="D5GK59"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.50.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36182">
        <property type="entry name" value="PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G10930)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36182:SF1">
        <property type="entry name" value="PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G10930)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="199"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="230"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="245"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="411" mass="41884" checksum="DDF5C20074DA73F2" modified="2010-06-15" version="1">MFEPKQWNVPEVVRATVEKSDGNLNPLQGDGSNFPCHGVAPEAPVATYLPGTSHPLKIKGSAVHGGGSGQMVITYETKPTKNTVFRVMTSYMGDHPIKAAGNLGPADRDPQGLKSELELWKLNPLSFTVPKGLPKGKAVVAWTWFNRIGNREMYMKCATVVIGGEETSMSVFEALPGMFKANIGGGCLVPEQTSAIDFKNPGPEVFGTGITPVPCGEPSSKGAGSGGSAPSGAGASGPAADTKPSVGNTIAPTNTSSPAPSQSAQTGAGNSGNGAGAQPPEGNIIEPPATTGGPAPSPSAPEGNGTGNEAVLATNIPVEPMPTATSEALAQTPIPSIPTTLMASTIRLSPPQQTGATPVEACTTEGEITCGENGTWYMCGSGRKQFMGSLAAGTTCTAGKIAKRGIRGYYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKC2</accession>
    <name>D5GKC2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00009471001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430337">
        <property type="protein sequence ID" value="CAZ84965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840774.1">
        <property type="nucleotide sequence ID" value="XM_002840728.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKC2"/>
    <dbReference type="STRING" id="656061.D5GKC2"/>
    <dbReference type="GeneID" id="9181985"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009471001"/>
    <dbReference type="eggNOG" id="KOG0725">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_654150_0_0_1"/>
    <dbReference type="InParanoid" id="D5GKC2"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050085">
        <property type="term" value="F:mannitol 2-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050664">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019594">
        <property type="term" value="P:mannitol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000090">
        <property type="entry name" value="NADP-dependent mannitol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008">
        <property type="entry name" value="BENZIL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008:SF4">
        <property type="entry name" value="CHAIN DEHYDROGENASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G08710)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ84965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="420" mass="45479" checksum="AEFD0D1DF4D093C0" modified="2010-06-15" version="1">MPCHSESTRPCGDCNGSSGDASLVARALTDAPNGNGTLVDVMFTGATCEDNLVDIPLAHPLVVGRDRLDSIQEPKLETGQKLALLPKVIRNFSLVGKVCVVTGGARGLGYNITEGFLQAGAQGAAILDILEDVGIKAADTLGKRYNTKVLFYKVDITNDESVEKVFSQICKDLGSIDVLLCSAGIADSNMPAETYSMARFRRLMDINVNGLMKCSQEAGKQMIEQRTGGSIVLIASMSSHIVNYPQEQCCYNASKAAVRHLGASLALEWAKHGIRVNSISPGYMDTELNRAPALEEQKVIWCDRTPMKRLGQEDELNNLALFLASPASSFVTGADVIIDIQYRVGIVFLRHHPNPQLDGLFLFTLPSIFYFLLSIFPLSPGFLGFECGETSWGICTGGIKIVSGSETNCEFGNLIWDINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKV2</accession>
    <name>D5GKV2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Trafficking protein particle complex subunit BET3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00009781001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 8">Golgi apparatus</location>
            <location evidence="1 8">cis-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the TRAPP small subunits family. BET3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430341">
        <property type="protein sequence ID" value="CAZ85145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840954.1">
        <property type="nucleotide sequence ID" value="XM_002840908.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKV2"/>
    <dbReference type="FunCoup" id="D5GKV2">
        <property type="interactions" value="1026"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GKV2"/>
    <dbReference type="GeneID" id="9183381"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009781001"/>
    <dbReference type="eggNOG" id="KOG3330">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087110_0_0_1"/>
    <dbReference type="InParanoid" id="D5GKV2"/>
    <dbReference type="OMA" id="MVQMQVQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030008">
        <property type="term" value="C:TRAPP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048193">
        <property type="term" value="P:Golgi vesicle transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14942">
        <property type="entry name" value="TRAPPC3_bet3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1380.20:FF:000001">
        <property type="entry name" value="Trafficking protein particle complex subunit BET3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1380.20">
        <property type="entry name" value="Trafficking protein particle complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016721">
        <property type="entry name" value="Bet3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007194">
        <property type="entry name" value="TRAPP_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13048">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04051">
        <property type="entry name" value="TRAPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018293">
        <property type="entry name" value="TRAPP_I_complex_Bet3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="Ligand-binding domain in the NO signalling and Golgi transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 8" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="7 8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR018293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ85145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="191" mass="21591" checksum="427F2A8F1C1F87D3" modified="2010-06-15" version="1">MSATKAARVGEDVWKTRIDKVNAELVTLTYGSIVAQLCRDFEGDYLEVNKQLEKMGYNIGLRLIEDFLAKTNMGKCANFRETAETIAKVGFKVFLNITPTVANWTTDNKQFSLIFEENPLADFVELPDDGRAQNELWYSNMLCGVLRGTLEMVQMQVDAHFASDILRGNEVTEMRVTLVRIIDDELPPGED</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>D5GL58</accession>
    <name>D5GL58_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00010017001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430346">
        <property type="protein sequence ID" value="CAZ85251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841060.1">
        <property type="nucleotide sequence ID" value="XM_002841014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GL58"/>
    <dbReference type="GeneID" id="9182045"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010017001"/>
    <dbReference type="eggNOG" id="ENOG502SG5T">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_364157_0_0_1"/>
    <dbReference type="InParanoid" id="D5GL58"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="600"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="711"/>
            <end position="723"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="767" mass="82844" checksum="B37F0991201CA084" modified="2010-06-15" version="1">MIEGKEDVIPQLQSRPSVRKNTYKGGNVYDNNDSENTNTKVANTENKVKNLNLKKDGDAASHVVDTEKVPVLDVTNKDKNINSKPKGNEEVAVVKKFEKNVEKNVNVDAGEPAAGPRGVPPVAIGSQRQGTTFLPGGVMPVGMTDIVFPDIIITNTNDINNANENQNQLASSNENQNQNPNDLVNENAAIAEQNFAENNFLFSEEKNFFYLNAPADVVDDLPPAEVVVGAPSPVTQVVQVTEVVQVESIHKPCITCPPTTEFVPITHVIPVETVVEQVQLPGQTITKQIQLPGETYVEQVQLPRETIIEKIQLPGQTYIEQLPGETVIEQIQLPCVTFYEQLPCETVTEKIVSPGETFIEQIVLPCETVTCEQEVTVQVTKTIPPTTVIKQESVIVENKVITTEAPKTVLESVYISKVIKVPVTVTKEKSIYIPEEVRIPVTVTKKNNVYISEEVKIPVTVVEEEIVEVYITKEVPVPTVISVPGIATPVATTVTKEVVLPGQVVTEFFPCVPTPVANTIKIPVTIKEKEEVYITQEVFVTKELVITSLVYVPGVKTPVPVTITKVVKATPRPSLEIPKAPTPAPAPEAEIECEVPTPETWAPISHAPTPNEKSFFGSEVGQAPSAPLERPKPDHGPVSQPLSAPFTPEVPSGEAEVGGQIPTPQCPATTEAEYPEPGSSKEIEVEFPIPKGEVEVSPPKPSRVPERSYPIPIPPAAPAPEAPSWPKETPGGAPETPLPPAFQGSASNNRPAITAVVAAILGAILAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>D5GL66</accession>
    <name>D5GL66_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00010027001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430346">
        <property type="protein sequence ID" value="CAZ85259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841068.1">
        <property type="nucleotide sequence ID" value="XM_002841022.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GL66"/>
    <dbReference type="STRING" id="656061.D5GL66"/>
    <dbReference type="GeneID" id="9182053"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010027001"/>
    <dbReference type="eggNOG" id="ENOG502SX0G">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_689274_0_0_1"/>
    <dbReference type="InParanoid" id="D5GL66"/>
    <dbReference type="OMA" id="CENEYVY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="400" mass="45701" checksum="94697D3B7D1290C3" modified="2010-06-15" version="1">MDKLPLETARHIVSYLTKRTDVASLRLVSRTFAILGAELLFHTISFHPSYPSLTRLCKISQRPHLAHNVRELVLNLPPIRDLIWGPFVRHLEAEGIDISGSTQRIQEAFRLLTYHVDQYSKLQTSGDLASMLGEAVRGLPRLRGVLMKENNERDRDYWLSQSSLTLGCNGGFNYRIELSAELHGYSGSKDHYRAFAALLDAVYYAGCKLDTFYVDAKVDPRMFFDNTVRLERAGAVFQNCRVLGLKMDIEDSMVEYLIKTPLFNVILPSSPNLQSLTLQFGISCAREAIPLSTIFGSKHVWPRLQKVSFSFIDMEDQELYAFLLRHKNTLRELSFSMCSLYAGRWDDAMMFLRQNLGLVALRLDPLFELAGEFDYLCESELKMMEDYVVNGGKCLPPRED</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GL99</accession>
    <name>D5GL99_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">beta-N-acetylhexosaminidase</fullName>
            <ecNumber evidence="3">3.2.1.52</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00010084001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.</text>
            <dbReference type="EC" id="3.2.1.52"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 20 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.52" evidence="3"/>
    <dbReference type="EMBL" id="FN430347">
        <property type="protein sequence ID" value="CAZ85292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841101.1">
        <property type="nucleotide sequence ID" value="XM_002841055.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GL99"/>
    <dbReference type="STRING" id="656061.D5GL99"/>
    <dbReference type="GeneID" id="9182086"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010084001"/>
    <dbReference type="eggNOG" id="KOG2499">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010969_1_0_1"/>
    <dbReference type="InParanoid" id="D5GL99"/>
    <dbReference type="OMA" id="GWQWTPA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004563">
        <property type="term" value="F:beta-N-acetylhexosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06564">
        <property type="entry name" value="GH20_DspB_LnbB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025705">
        <property type="entry name" value="Beta_hexosaminidase_sua/sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052764">
        <property type="entry name" value="GH20_Enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015883">
        <property type="entry name" value="Glyco_hydro_20_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43678:SF1">
        <property type="entry name" value="BETA-N-ACETYLHEXOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43678">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_2G00640)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00728">
        <property type="entry name" value="Glyco_hydro_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00738">
        <property type="entry name" value="GLHYDRLASE20"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072416" description="beta-N-acetylhexosaminidase" evidence="6">
        <location>
            <begin position="19"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 20 catalytic" evidence="7">
        <location>
            <begin position="166"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR625705-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ85292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="701" mass="78448" checksum="4B8F5C773AC0CAE5" modified="2010-06-15" version="1">MQLLVLLFLTLSLLGAEAYNALPPVPWRYSTGTFKFSLRRPPTIYLQESAAEARDTDGLTLIPPSVREFAETFAGDLKDRFHVDVRVECVAFERGRAGPGIYLGIDEGPGKYTYESGIETSEGYTISVAEDSELMLAREVGGVHPGDRRDEVIELKAGRAVDSPAYPTRGFLLDAGRKWYSADFLKDLCTYASFFKFSEFHYHASDNYPLNRGRNSTWQNIYSQFSLIPDNVALHPLVARHNESHSRAEFESIQRHCASRGITIIPEIEAPGHALAITKWKPELALTNKKDLLNLTHPDTIPTVKAIWSEFLPWFQTKEVHIGADEYDSDLADVYIHFVNTMSEFIFTTAGKKVRIWGTHEPSNTSVISRAITTQHWQYGQSDPVQLERDGYEIINSEDWWGYTGLKNDHTPILPAPYPQYFNEARVLNFGGVNGWQWEPSLYNPFNTTAEWQIPIESKRNKGAIMATWNDNGPDATTQLEAYYCIRDGIPVVGARSWSGHRGPEIDVGTLEISKSILTNNAPGQNLDRRLGGGYNLGDLLFDWPTGTLGVRLATKSKGMNYTMQITYTSPFTLSSAHDAVSLTLTPDSTLIFTADGFEYPLRSVAESAFDPSHPGRIWSNDTASTHIPVSLPPIATITIQTDRIGGSRVWQNGTFLGRFEVFIFGGRNTLFSWSQMAFVAPVEQVRGGVHRVRIWEGLVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLI9</accession>
    <name>D5GLI9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Nitrate reductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">GSTUM_00010228001</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 16">Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>nitrite + NADP(+) + H2O = nitrate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19061"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16301"/>
            <dbReference type="ChEBI" id="CHEBI:17632"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.7.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>Mo-molybdopterin</name>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
        </cofactor>
        <text evidence="17">Binds 1 Mo-molybdopterin (Mo-MPT) cofactor per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the nitrate reductase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430349">
        <property type="protein sequence ID" value="CAZ85382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841191.1">
        <property type="nucleotide sequence ID" value="XM_002841145.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLI9"/>
    <dbReference type="STRING" id="656061.D5GLI9"/>
    <dbReference type="GeneID" id="9186126"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010228001"/>
    <dbReference type="eggNOG" id="KOG0534">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0535">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0537">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003827_4_0_1"/>
    <dbReference type="InParanoid" id="D5GLI9"/>
    <dbReference type="OMA" id="KAMMPDY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030151">
        <property type="term" value="F:molybdenum ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
        <property type="term" value="F:molybdopterin cofactor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050464">
        <property type="term" value="F:nitrate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008482">
        <property type="term" value="F:sulfite oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042128">
        <property type="term" value="P:nitrate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006809">
        <property type="term" value="P:nitric oxide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006790">
        <property type="term" value="P:sulfur compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06183">
        <property type="entry name" value="cyt_b5_reduct_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000021">
        <property type="entry name" value="NADH-cytochrome b5 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.120.10:FF:000016">
        <property type="entry name" value="Nitrate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.650">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.420.10">
        <property type="entry name" value="Oxidoreductase, molybdopterin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008333">
        <property type="entry name" value="Cbr1-like_FAD-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005066">
        <property type="entry name" value="MoCF_OxRdtse_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008335">
        <property type="entry name" value="Mopterin_OxRdtase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012137">
        <property type="entry name" value="Nitr_rd_NADH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000572">
        <property type="entry name" value="OxRdtase_Mopterin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036374">
        <property type="entry name" value="OxRdtase_Mopterin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022407">
        <property type="entry name" value="OxRdtase_Mopterin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19372:SF7">
        <property type="entry name" value="SULFITE OXIDASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19372">
        <property type="entry name" value="SULFITE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00970">
        <property type="entry name" value="FAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03404">
        <property type="entry name" value="Mo-co_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00174">
        <property type="entry name" value="Oxidored_molyb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000233">
        <property type="entry name" value="Nitr_rd_NADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00406">
        <property type="entry name" value="CYTB5RDTASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00363">
        <property type="entry name" value="CYTOCHROMEB5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00407">
        <property type="entry name" value="EUMOPTERIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56524">
        <property type="entry name" value="Oxidoreductase molybdopterin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00559">
        <property type="entry name" value="MOLYBDOPTERIN_EUK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0274">FAD</keyword>
    <keyword evidence="8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0349">Heme</keyword>
    <keyword evidence="12" id="KW-0408">Iron</keyword>
    <keyword evidence="9 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 17" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="13 16" id="KW-0534">Nitrate assimilation</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="19" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072423" description="Nitrate reductase" evidence="19">
        <location>
            <begin position="21"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="20">
        <location>
            <begin position="557"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="21">
        <location>
            <begin position="658"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="40"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="70"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>Mo-molybdopterin</name>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
        </ligand>
        <ligandPart>
            <name>Mo</name>
            <dbReference type="ChEBI" id="CHEBI:28685"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR000233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR000233-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="CAZ85382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="913" mass="101002" checksum="7CF5D7A10CAFC370" modified="2010-06-15" version="1">MNTLDTVFGWLLSLLSLIRFFRTTKKLVIDGTTSSISIPNVETNPHIPPSNAFLGNPNQLAPPTPPESDKGDDECDDLENIPLPPPSVNPTTVLDVDVGTPDSHVPRDPRLIRLTGVHPFNVEAPLSALFDSGMIFDPSGTLLFHDDEMLDWTITIEGLVANPLALTLRQIITGFEQVTLPVTLVCAGNRRKEQNQVRKSQGFSWGAAGVSTSLFTGPLLASVIAAARPQRNARYLCMEGADELPNGNYGTSIRLSQVFDPNRGIMLAHKMNGEELRPDHGRPLRVVVPSMIGGRSVKWLKKIILTESPSENWYHIYDNRVLPTMVTPEMAKANKDWWTDERYAIYDLSVNSAIAYPAHDETLSMETTKEQYNLKGYAYAGAGRRITRVEISLDKGRSWILSSVEYPEDLYRSIPENATLFGGRLDVGSRDACLCWCFWSLDIPTSALFAADDIVVRAMDDSMNTQPRDTYWSVLGMMHNSWFRVAIRKENGRIRFEHPNQPALLKGGWMERVKKEGGDLLGLNWGERVMGEESVAGGATSAPEVRDTVKMTDDSVQRTIEIEELRGHDNASEPWFVVEGEVYDGKPFLEEHPGGATSIISAAGQDATDEFVGIHSESAKAMMPKYHIGTLSATARKLLLEPAAPSKKGPSETFLDPRAWCQAALVQKKVVSWDTRIFTFELDNPTQTLGLPVGQHLLIKIRDEKTGEVITRPYTPISCNTEKGVVHLLVKVYFDTPTALGGKMTQAMDRMQMGHTASFKGPIGKLVYHGQGVVSLLDNRINVTSFLMICGGSGITPIFQVLRAVLSDPTDSTTCVVLDGNRAEEDILCREDLDSFAATYPEKCKIVHTLSKPSERWQGERGRIGWDLIKRNYPQGCEGRKPLALICGPESLEKSVRGLLTAGGWGESDMVFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GM45</accession>
    <name>D5GM45_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">polynucleotide adenylyltransferase</fullName>
            <ecNumber evidence="3">2.7.7.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00010521001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA polymerase type-B-like family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.19" evidence="3"/>
    <dbReference type="EMBL" id="FN430352">
        <property type="protein sequence ID" value="CAZ85588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841397.1">
        <property type="nucleotide sequence ID" value="XM_002841351.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GM45"/>
    <dbReference type="STRING" id="656061.D5GM45"/>
    <dbReference type="GeneID" id="9187372"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010521001"/>
    <dbReference type="eggNOG" id="KOG1906">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013572_2_0_1"/>
    <dbReference type="InParanoid" id="D5GM45"/>
    <dbReference type="OMA" id="CERIGHD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031499">
        <property type="term" value="C:TRAMP complex"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990817">
        <property type="term" value="F:poly(A) RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010605">
        <property type="term" value="P:negative regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043634">
        <property type="term" value="P:polyadenylation-dependent ncRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05402">
        <property type="entry name" value="NT_PAP_TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.10:FF:000006">
        <property type="entry name" value="non-canonical poly(A) RNA polymerase PAPD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054708">
        <property type="entry name" value="MTPAP-like_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002058">
        <property type="entry name" value="PAP_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045862">
        <property type="entry name" value="Trf4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23092:SF15">
        <property type="entry name" value="INACTIVE NON-CANONICAL POLY(A) RNA POLYMERASE PROTEIN TRF4-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23092">
        <property type="entry name" value="POLY(A) RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22600">
        <property type="entry name" value="MTPAP-like_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03828">
        <property type="entry name" value="PAP_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81631">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Poly(A) RNA polymerase mitochondrial-like central palm" evidence="9">
        <location>
            <begin position="391"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="domain" description="PAP-associated" evidence="8">
        <location>
            <begin position="591"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="187"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="749"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="20"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="59"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="249"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="301"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="317"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="749"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="781"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="793"/>
            <end position="802"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF22600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ85588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="802" mass="89943" checksum="F29ED34B4F315C75" modified="2010-06-15" version="1">MGDRGRDRRVNDIHRFTGRSDPNNPNHEFTYQHQRQMPTETDSYRPSNRSQDSNQGPTRHLKGSKGSKTGKRQKTSQQPKPQKWTPVPAHERPILSFNVREKTPDLLPGMITGGEFLSAPDGSGDGIGESIRDAAGADDEVEMDMSDDENPSLAVTKVGSATEGAVDGEKKPDVISLVRSFKARATMGKPKNPIQDNDDFISLSFGDEPSHLGQGDEDSNHKRRKVNGKDNCDEPSFSHRYNHFGPPKDTGPPGLPPRPLTYRKPSPPPQLRDLDPQPRNRSVRQSNNGGPSSLPGAAHSKYTSSNTSRNPAMQNKGYDRQLDIPKNRKRSYSDMNRGKTTTDGNITYEYMATPRSDPFPWTKMGRDHSRSLIILLENENESNSFCAIYRLHNEILDFIAYIQPRAYEHAVRRELVHRVRVVVTKLWPDTDLRVFGSFAAELYLPTSDIDLVVISTSFAKIGIPRYSERNALYKLRQAILSSNTAKPGSLAVIANAKVPIIKFVDRETNIHVDISFENSSGLVANETFIRWKAQYPAMPILLTVIKQFLAMRGLNEVYLGGLGSFSVTCLIVSMFQMHPGAASGRMDPRRHLGVMLLEFLELYGKSFNTETLGICVDSRKPSYFRKNDVFPVIQRGATPPQTDLLCIQDPNNSENDISRSSYQIKIILTLWAEALDDLVGQMKKFERLQFVNRQNRSLLGVILGGSYGKIEEQRWLMRKVYMDRIGPLEDILTEEKQWGEATPFVTRTLSPVQQVTNRSSGSGPVPNKKTSHGSNSSGPSHPRKSKQGFCRKGRQDEPIVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GM54</accession>
    <name>D5GM54_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00010532001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430352">
        <property type="protein sequence ID" value="CAZ85597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002841360.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="9187381"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010532001"/>
    <dbReference type="HOGENOM" id="CLU_2639894_0_0_1"/>
    <dbReference type="InParanoid" id="D5GM54"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="77" mass="9237" checksum="184EA66B48FEA87A" modified="2010-06-15" version="1">MRHWNCVNGYIYHHHHCPFYSVKQKQPRCTRAQHSASSGGGKQSRLGNSQFCPPTHFYSRPNRKLMWRFIDPRRTEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5GM70</accession>
    <name>D5GM70_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00010550001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430352">
        <property type="protein sequence ID" value="CAZ85613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841422.1">
        <property type="nucleotide sequence ID" value="XM_002841376.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GM70"/>
    <dbReference type="GeneID" id="9187397"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010550001"/>
    <dbReference type="HOGENOM" id="CLU_2499513_0_0_1"/>
    <dbReference type="InParanoid" id="D5GM70"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ85613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="86" mass="10236" checksum="671870B4879306C7" modified="2010-06-15" version="1">MILFCLFAFNDRFPSHLFAPFHVQELCRSELAIFPFKYVLIFIFIFPLPYESECKCYELGRKLWTSSSPGNYPVVSALRYRYHLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5GM99</accession>
    <name>D5GM99_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Anaphase-promoting complex subunit 5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Cyclosome subunit 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00010617001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. The APC/C complex catalyzes assembly of branched 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on target proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the APC5 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430354">
        <property type="protein sequence ID" value="CAZ85642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841451.1">
        <property type="nucleotide sequence ID" value="XM_002841405.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GM99"/>
    <dbReference type="STRING" id="656061.D5GM99"/>
    <dbReference type="GeneID" id="9183221"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010617001"/>
    <dbReference type="eggNOG" id="KOG4322">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010478_0_0_1"/>
    <dbReference type="InParanoid" id="D5GM99"/>
    <dbReference type="OMA" id="DANMGMA"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045842">
        <property type="term" value="P:positive regulation of mitotic metaphase/anaphase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070979">
        <property type="term" value="P:protein K11-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037679">
        <property type="entry name" value="Apc5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026000">
        <property type="entry name" value="Apc5_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12830">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12830:SF9">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12862">
        <property type="entry name" value="ANAPC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Anaphase-promoting complex subunit 5" evidence="9">
        <location>
            <begin position="250"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ85642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="739" mass="83843" checksum="43247CF2D9A33666" modified="2010-06-15" version="1">MSRYLTSGKIALLALVELYAESRFPNQSVVPVLSFVIRHLVSSKENTAFILTLENIKSATLHQPSAVVGRTIFDLLLKKLWEINSFDALHTFFANIQTYLIEPERADKSPVRVHGEKSMLSRTSVLGCFVRRAALEYVRLQFHDAVSLWRSFIAYREPTLAMWKKRNLGAGPMSFDANLKGLGFGDGMVGVVYGKGKESRDCTVSTDDVERLLEFQVDSMQRMGTRIPDDMQVQLRQMLSTNVTIPALTHYVEFLNSWRSGDYPGSFDNLHRYFDYTMHNRDRTFYQYALLNLAILQADFGCYRESILAMQETINTARENKDMACLNFSLSWLYHFHKAHPQDCPDVISSRMERESLQFLKAKAKEGGMHHLQSMAHLSEAKQILVGGESIPAAFESILRSSHLNVTKNIHNAMGSQILLQSSLWSRMGISYLSWMNCELFLSKYRVNSPIEDVVKALCRSAYIMSLRGKTDEALERLDQVDQECLRTLKVYQYWATYVGLLKLRQAMYRGDAAAAELLLQQLSASPYVEPDCALEISVCRLDLLARRGNYSAALTAITQLSEELVRDRADTYHRIRVMTIKADLLSKCGRTVKGLSVAISAATIAWRSRLLPALFQAFRTIANILIHMGEFSAAYSMMDNIMPQVLECEDAYLNAQCFSCLADAQMGLAGTKKGAGRNECLHRALEFIDRGFSEYSRIRDTKSQMTLMSQKARIQDFTGEKSLRDDAVKWYKQLKKGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMW2</accession>
    <name>D5GMW2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 10">Vacuolar protein 8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00010964001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 10">Functions in both vacuole inheritance and protein targeting from the cytoplasm to vacuole.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Vacuole membrane</location>
            <topology evidence="1 10">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the beta-catenin family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430360">
        <property type="protein sequence ID" value="CAZ85855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841664.1">
        <property type="nucleotide sequence ID" value="XM_002841618.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMW2"/>
    <dbReference type="FunCoup" id="D5GMW2">
        <property type="interactions" value="110"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GMW2"/>
    <dbReference type="GeneID" id="9183423"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010964001"/>
    <dbReference type="eggNOG" id="KOG4224">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021483_0_0_1"/>
    <dbReference type="InParanoid" id="D5GMW2"/>
    <dbReference type="OMA" id="VWDKPDG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071563">
        <property type="term" value="C:Myo2p-Vac17p-Vac8p transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071561">
        <property type="term" value="C:nucleus-vacuole junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043495">
        <property type="term" value="F:protein-membrane adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051656">
        <property type="term" value="P:establishment of organelle localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061724">
        <property type="term" value="P:lipophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071562">
        <property type="term" value="P:nucleus-vacuole junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000425">
        <property type="term" value="P:pexophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903044">
        <property type="term" value="P:protein localization to membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034497">
        <property type="term" value="P:protein localization to phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031503">
        <property type="term" value="P:protein-containing complex localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034517">
        <property type="term" value="P:ribophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000011">
        <property type="term" value="P:vacuole inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000243">
        <property type="entry name" value="Putative Vacuolar protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000095">
        <property type="entry name" value="Vacuolar protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045156">
        <property type="entry name" value="Vac8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47249">
        <property type="entry name" value="VACUOLAR PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47249:SF1">
        <property type="entry name" value="VACUOLAR PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 10" id="KW-0926">Vacuole</keyword>
    <feature type="repeat" description="ARM" evidence="9">
        <location>
            <begin position="90"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="9">
        <location>
            <begin position="131"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="9">
        <location>
            <begin position="172"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="9">
        <location>
            <begin position="213"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="9">
        <location>
            <begin position="256"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="9">
        <location>
            <begin position="297"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="9">
        <location>
            <begin position="338"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00026209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU369097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ85855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="561" mass="60450" checksum="26ED9D9E35F003F5" modified="2010-06-15" version="1">MGNCGSSCSTCCRGRTRDGLYEPVLQESEREAVSDLLQYLENRSEVDFFTGEPLQALSTLVYSDNVDLQRSASLTFAEITERDVREVDRDTLEPILFLLQSSDIEVQRAASAALGNLAVNTENKVAIVLLGGLAPLIRQMMSPNVEVQCNAVGCITNLATHEDNKAKIATSGALGPLTRLAKSKDMRVQRNATGALLNMTHSDDNRQQLVNAGAIPVLVSLLSSGDVDVQYYCTTALSNIAVDQANRKKLASNEPKLVFSLVHLMDSSSPKVQCQAALALRNLASDEKYQLDIVRAKGLLPLLRLLQSSFLPLILSAVACIRNISIHPLNESPIIEAGFLRPLVDLLGSTENEEIQCHAISTLRNLAASSDKNKELVLEAGAVQKCKQLVLGVPLSVQSEMTAAIAVLALSDDLKSHLLNLGVFDVLIPLTASESIEVQGNSAAALGNLSSKVGDYSMFVQDWLEPEGGIHGYLKRFLASGDPTFQHIAIWTLLQLLEADDTRLKNLIGQSTEIVTMIRNIADKNIDSDDEGDDGEGEVVALARRSADLLSGGSGTFGKAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNM4</accession>
    <name>D5GNM4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">allantoicase</fullName>
            <ecNumber evidence="3">3.5.3.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00011373001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Utilization of purines as secondary nitrogen sources, when primary sources are limiting.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>allantoate + H2O = (S)-ureidoglycolate + urea</text>
            <dbReference type="Rhea" id="RHEA:11016"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16199"/>
            <dbReference type="ChEBI" id="CHEBI:17536"/>
            <dbReference type="ChEBI" id="CHEBI:57296"/>
            <dbReference type="EC" id="3.5.3.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Nitrogen metabolism; (S)-allantoin degradation; (S)-ureidoglycolate from allantoate (aminidohydrolase route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the allantoicase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.3.4" evidence="3"/>
    <dbReference type="EMBL" id="FN430366">
        <property type="protein sequence ID" value="CAZ86117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841926.1">
        <property type="nucleotide sequence ID" value="XM_002841880.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNM4"/>
    <dbReference type="FunCoup" id="D5GNM4">
        <property type="interactions" value="50"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GNM4"/>
    <dbReference type="GeneID" id="9186193"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011373001"/>
    <dbReference type="eggNOG" id="KOG4145">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038797_0_0_1"/>
    <dbReference type="InParanoid" id="D5GNM4"/>
    <dbReference type="OMA" id="MDDGWET"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004037">
        <property type="term" value="F:allantoicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000256">
        <property type="term" value="P:allantoin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006144">
        <property type="term" value="P:purine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000078">
        <property type="entry name" value="DAL2p Allantoicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000059">
        <property type="entry name" value="Probable allantoicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00813">
        <property type="entry name" value="Allantoicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005164">
        <property type="entry name" value="Allantoicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015908">
        <property type="entry name" value="Allantoicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02961">
        <property type="entry name" value="allantoicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12045">
        <property type="entry name" value="ALLANTOICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12045:SF3">
        <property type="entry name" value="INACTIVE ALLANTOICASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03561">
        <property type="entry name" value="Allantoicase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016516">
        <property type="entry name" value="Allantoicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0659">Purine metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Allantoicase" evidence="8">
        <location>
            <begin position="31"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="Allantoicase" evidence="8">
        <location>
            <begin position="200"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00056910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00060607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ86117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="347" mass="37839" checksum="4644056F1FBA38E6" modified="2010-06-15" version="1">MSSNTAHRIDESEAQEAFMSSCIDLISSALGGQVIEFSDQWFAAAENLINPAPPIGRPGVFVPTGAWYDGWETRRHNKAPADWVIIKLGVASGRVHGFEVDTAFFKGNYAPAVTVEGTFLDGSHPDTSTVWEEILPMQECGPSQRHIWKLSSPTTKAYSHVRLNMFPDGGIARFRLFGTAVPVFPSDPDAIIDLAHVSSGGLAISCSEQNFGTKKDNLLLPGRGKNAGNGWETKRSREPGHVDWVVVKLGAPGYIEEVIVDTLHFRGNYPQAVEIYAINSPEQHVAGDANGWELIVPAQACEADKEHAFPSAFLQNVNGKVVTHVKMVIIPDGGVKRLRVFGKRARN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNN5</accession>
    <name>D5GNN5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00011387001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430366">
        <property type="protein sequence ID" value="CAZ86128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841937.1">
        <property type="nucleotide sequence ID" value="XM_002841891.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNN5"/>
    <dbReference type="FunCoup" id="D5GNN5">
        <property type="interactions" value="25"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GNN5"/>
    <dbReference type="GeneID" id="9186204"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011387001"/>
    <dbReference type="eggNOG" id="KOG2273">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007739_1_1_1"/>
    <dbReference type="InParanoid" id="D5GNN5"/>
    <dbReference type="OMA" id="MGWLEGI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06869">
        <property type="entry name" value="PX_UP2_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047168">
        <property type="entry name" value="LEC1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024554">
        <property type="entry name" value="LEC1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024555">
        <property type="entry name" value="PX-associated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47185">
        <property type="entry name" value="PX DOMAIN-CONTAINING PROTEIN YPR097W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47185:SF1">
        <property type="entry name" value="PX DOMAIN-CONTAINING PROTEIN YPR097W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12825">
        <property type="entry name" value="DUF3818"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12828">
        <property type="entry name" value="PXB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="2">
        <location>
            <begin position="190"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="269"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="805"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="841"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="277"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="841"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="882"/>
            <end position="894"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ86128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="914" mass="104405" checksum="65FF07A061C4F651" modified="2010-06-15" version="1">MTAPAPPDLSSLPISGKKNILAGKQEHYLKRELVSRQVQFEISELNSPTALQRFGAPFRSPLGEVAPVDSELPILRYIFVHHVRNFPFLDQAREKEFWQDRLQVFLESFANKRISSSEDRLEETKRRKLALKCEKVVALMMNSGIPTASGYEESVGFDEIEAAGGADRAASENGILVNMTEGHWVNGWDVNVASVRQTSIKRTVRYHTHAEFLIRTKRMDQPDVIVGRRYGAFARLHRNLRNELPGKVLPALPRKINKSQTSTMGLLSMSSADDRSSTYSVSTTSSNSVNANGEKGGSGHKRSQSLGASLGISKHSRNASRNSLASSINEPVTLWRENQRISLRAFLRVLLGDAQVAKSRAMNDFLLKDPITLNEEELNDEARRREMDHIRILEQQKFYEIARQRARELDVYMESFRREIVESNGLTKLFAEIREKDKLEDLSLQYKKFAEWLRIEVAATIYHIFLAEDNSPELFHQAKRIHSLIPYTILKNVIRIANPAAVMTGVLDLFLATPFGSRSLMQRILSLAINDGIRQLQKSIDSLTVKINDPVLCSKLRQFTEIDEETREEIRMDAVLDQVDLIVAILRSERIEPELEPKQVETIFNAYVGWNNAVENIDDMRAGGQLFAYLKQLLKLYTRQRDKNMMLAIIGEPVTLQLFRDLFTIFYEPLIRVYKSANVYNSVTDFAVFADDMVEVKETLTVESIDMSSDPNQTVQAFIDLCARHENNFYKFVHEVHIHDDGLFEKLMGWLEGILSFLRSGPKSGPLDMNRLVEEAVLRGDIDGNKAKEEIDQLIKWQAQRRKWHEDKTRQKMASGGDMNGWLNTAPDNAFTTSDFHLNETDLDDLNNVDDDNNSSDEDESDDQADPIEAEKRRRKKKQDHLRRSAGEPEKPEISEILKLQVEFLLQLRNVLAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNQ5</accession>
    <name>D5GNQ5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00011428001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430367">
        <property type="protein sequence ID" value="CAZ86152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841961.1">
        <property type="nucleotide sequence ID" value="XM_002841915.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNQ5"/>
    <dbReference type="GeneID" id="9182787"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011428001"/>
    <dbReference type="HOGENOM" id="CLU_1732822_0_0_1"/>
    <dbReference type="InParanoid" id="D5GNQ5"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ86152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="151" mass="16849" checksum="C74161D5D9F70189" modified="2010-06-15" version="1">MSSKEKPGSPAPSSSQEPKDEDEEEMDLINAFMSGEKTFYSPLPPVKPTPPKPSGSRKRVAFAEPREEPSKAPKIEHRQISMPAELMVPNSRRTPWKAPKHHSLPCTLKRQNPNYWWSAVTPEHRIPPVLNENESGSNSRLGNSSQGRLGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNU1</accession>
    <name>D5GNU1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00011480001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430368">
        <property type="protein sequence ID" value="CAZ86184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841993.1">
        <property type="nucleotide sequence ID" value="XM_002841947.1"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GNU1"/>
    <dbReference type="GeneID" id="9182819"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011480001"/>
    <dbReference type="eggNOG" id="KOG1202">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_35_3_1"/>
    <dbReference type="InParanoid" id="D5GNU1"/>
    <dbReference type="OMA" id="AHMGVGT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004312">
        <property type="term" value="F:fatty acid synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031177">
        <property type="term" value="F:phosphopantetheine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08955">
        <property type="entry name" value="KR_2_FAS_SDR_x"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00833">
        <property type="entry name" value="PKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.366.10">
        <property type="entry name" value="Malonyl-Coenzyme A Acyl Carrier Protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014043">
        <property type="entry name" value="Acyl_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016036">
        <property type="entry name" value="Malonyl_transacylase_ACP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032821">
        <property type="entry name" value="PKS_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013968">
        <property type="entry name" value="PKS_KR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050091">
        <property type="entry name" value="PKS_NRPS_Biosynth_Enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020806">
        <property type="entry name" value="PKS_PP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006162">
        <property type="entry name" value="Ppantetheine_attach_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775">
        <property type="entry name" value="FATTY ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775:SF22">
        <property type="entry name" value="SYNTHASE, PUTATIVE (JCVI)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00698">
        <property type="entry name" value="Acyl_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16197">
        <property type="entry name" value="KAsynt_C_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08659">
        <property type="entry name" value="KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00827">
        <property type="entry name" value="PKS_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00823">
        <property type="entry name" value="PKS_PP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01294">
        <property type="entry name" value="PKS_PP_betabranch"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55048">
        <property type="entry name" value="Probable ACP-binding domain of malonyl-CoA ACP transacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="KS3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52004">
        <property type="entry name" value="KS3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00012">
        <property type="entry name" value="PHOSPHOPANTETHEINE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="1" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ketosynthase family 3 (KS3)" evidence="7">
        <location>
            <begin position="1"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="Carrier" evidence="6">
        <location>
            <begin position="1310"/>
            <end position="1385"/>
        </location>
    </feature>
    <feature type="domain" description="Carrier" evidence="6">
        <location>
            <begin position="1424"/>
            <end position="1502"/>
        </location>
    </feature>
    <feature type="domain" description="Carrier" evidence="6">
        <location>
            <begin position="1540"/>
            <end position="1618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1279"/>
            <end position="1305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1389"/>
            <end position="1422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1506"/>
            <end position="1537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1282"/>
            <end position="1294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1295"/>
            <end position="1305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1389"/>
            <end position="1400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1506"/>
            <end position="1516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS52004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ86184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1620" mass="173644" checksum="50BFCB8EC718B2B0" modified="2010-06-15" version="1">MDPQQRICLEVAWEALEHAGISPNDIAGSDAAVLMGVNSDDYGRLMLEDLPGIEAWMGVGSSFCGVPNRISYLLDLHGNSIALDAACASSLIAIHHAKQLLENYESEVAVVGGVNAMCGPGLIRVLDVAGATSPEGRCRSFDAEARGYGRGEGAAVVIMKRVEDAIRDGDQILSVVKGTAVGQDGRTNGIMQPNPQAQAEVYKKALSAAGFDASTIDFVEAHATSTRVGDPKEVSAITSVYGSAAGRTAENPCYIGSVKPNVGHLEAGAGVAGVIKATLALHYRQLPPQANLQTLNPAVDWASSGLQVVRDARPWPRPDQKLRAGVCSYGYGGSISHLVLEEAPSREALKEENNGPVILTLSGPGEKWYKGQAAKLAEWLKTEGAKIPLSAIATTLSTRRAHHHVRASIVAENHEGAIASLEKLSRGIDDLTIIKARTVSGNTNNDVVWVFSGHGAQYPSMGVELLKTEPEFAKVIDTLESTYQETLGFSPRQALETGDFETTDKIQALTFAMHVGLAAVLKSRGIKPAAIIGHSVGEIAAAVTTGALTLLEGARVASIRASLYGRPDVAGKGAMILVDIPFEDVEKDCASRKDINASIHASPTSTVVSGTPEAIKEYSDYLATQQQQVRVVKSDVAFHSPLLTFLGPALTRLLGDTISPKQADIKLYSTSDLDARTDKLRNCDYWINNMLGPVWLTSAVKAAAEDGYKVFLEVSAHPLVSHSINETLMEIGEDESVVIPTIRRNHPAWEGINTALGRLHANGAVVDFKKFVGKTWVPSLPLTVWQHEKYWHEVTLDGTPGVSGSVESLAHSLHWTAAQLDSTPAPLNNVVFVANKDHSLLGPFKKTLTKRGLGVKVIESPSEIESHSDVLHKTGTVVIVLPDELEDIERLPQTNFANTLKLLEITKRLNTFNSSAKLWVITEEAQNAKNEYSLAAASMLGLARIIASEQPDLWGGLVDIDEPAFPFQVLKFIRKAGVVIVRDGVSKIPRLRALPKASLTPGSDLKSAVKIRPDGTYLITGGLGVLGLEVASWLAEKGARRLVLLSRKGLPLRSTWSSATSGPNAGIINKVKTIEALGVAVNTVSLDLGKPGADEDLKKALDNLSLPPVTGVVHAAGVMEDAFTHEATEQSFKNVLTPKVDGSWALHNAFPVGTLDFFALFSSCGQLFGFPGQGAYASGNAFLDALANYRRNSGDNTLSLMWTAWSGLGMSASTSADYIDAEMESKGITSVTRDEAFRAWEYAAKYDVPQVVVVRSLPIDEGATPAVPILNDIVARRPAPEVDSEDEENSEYEESAPAATTPSNPADLKKHLEATIKANVASVLRLSSPDDVDTHRPLSELGMDSVMTVGLRKQFKDTFKVTIPPTLIWAHPTVAHLTKWFAENAKVSAPSAAATTSSAPKKSKAPKKSTPSSSGNALPSNPVDLKKRLEQMIKADVASVLRLSSPDDVDTHRPLSELGMDSVMTVGLRKQFKDTFKVTIPPTLIWAHPTVAHLTKWFTENVKPTSSAAAAAAPPTKKSKSTKKSSTSPTGLSLPTNPAELKKHLEKQIKADVASVLRLSSPDDVDPHRPLSELGMDSVMTVGLRKQFKDTFKVTIPPTLIWAHPTVSHLCKWFAENVAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPN3</accession>
    <name>D5GPN3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00011945001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430377">
        <property type="protein sequence ID" value="CAZ86476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842285.1">
        <property type="nucleotide sequence ID" value="XM_002842239.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPN3"/>
    <dbReference type="FunCoup" id="D5GPN3">
        <property type="interactions" value="46"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GPN3"/>
    <dbReference type="GeneID" id="9186716"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011945001"/>
    <dbReference type="eggNOG" id="ENOG502QQWB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008201_1_0_1"/>
    <dbReference type="InParanoid" id="D5GPN3"/>
    <dbReference type="OMA" id="WSQKQPE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000935">
        <property type="term" value="C:division septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04399">
        <property type="entry name" value="RhoGAP_fRGD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000044">
        <property type="entry name" value="Rho-gtpase-activating protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000050">
        <property type="entry name" value="RhoGAP and Fes/CIP4 domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000591">
        <property type="entry name" value="DEP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065">
        <property type="entry name" value="PROLINE-SERINE-THREONINE PHOSPHATASE INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065:SF17">
        <property type="entry name" value="RHO-GTPASE-ACTIVATING PROTEIN RGD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00610">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00049">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50186">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-BAR" evidence="5">
        <location>
            <begin position="2"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="DEP" evidence="3">
        <location>
            <begin position="225"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="4">
        <location>
            <begin position="460"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="164"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="292"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="691"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="165"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="696"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="726"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="752"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="793"/>
            <end position="805"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ86476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="852" mass="93797" checksum="F047533654998C31" modified="2010-06-15" version="1">MPGFADSFWSGDYAGGLGVLFGKLQQGVIENQQILQISKLRADAEDAYGQRLATIPAGAEKPGGFGRDDGASVRKAFDGLRKEMDDAGKNHRKVADNIRQLVINPFSKWCEAHGERIINSHDDLQAQIKSHDKQAEAVKKLRSHYFNKCRLVEDLEEETKFIQVPAPGTPSSATAPPTAPAADPEDEEPLEIGDLFYQPAEVKKMLAHMLETIPLGEVKVAILGTYQNVSTGENIVKFIQENLGATSISHAERIGQDLVTHGFLRLVGSVGNTFANSSKLHYQWRPKTFQLTGKPEKPAQRSVRPLENGGDTAESPTMAYVGDYLGGLLTNQHPGETPGDRLRREASEADERYKAGVKKLDLMRCALEESMMEHLKFMERCELDRLKAIKSVILDLSGAISNVIPSIQATVDNMLLYQETIQPLGDLRYMLESYRTGSYVPKVVTYESYYNSVDEQTFGVDLEARARADRKRVPIIITSILTYLDHHYPDLEGDEARRGIWLVDVPLAATHHLRNTINTGKAIPKEALATYEIPIVASVLKLYLLELPDTLVSSHKYEIIKTVYTTHGTEGEEKQRLAVIQNTLGSLPLTNIATLDAITTHFTRLIELTSADEAYVTTLAQSLSSCILRPRVESSLTQHERYAYRLVRDLFAHKEQIFGELKRASTLNSGRARAVSTDESQRRAFVEARNRAVIGAARSRPSSPAPSSRHRREVSTDGGNRFPIQTSPTSGSPRGSRYRPSSLEVPGGASSLDRDQKLDSEAEAPRVSGEPESFAHHESGESSPATPTAGLEKSNSLTRKSQAGGSTRFPRKGANLVRSAAGGPKRESLTMGGSFEEDRPAGVSLTDRPMDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPT6</accession>
    <name>D5GPT6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00012025001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430378">
        <property type="protein sequence ID" value="CAZ86529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842338.1">
        <property type="nucleotide sequence ID" value="XM_002842292.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPT6"/>
    <dbReference type="GeneID" id="9182273"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012025001"/>
    <dbReference type="HOGENOM" id="CLU_1587694_0_0_1"/>
    <dbReference type="InParanoid" id="D5GPT6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ86529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="168" mass="17694" checksum="30039C41D113A1F4" modified="2010-06-15" version="1">MPYRWRCCKCDNGWMTVQAEKNVCTWSECQHVKCNGCDQRNMAAPLYSGGDIIKDITGGGGGRGIGGFAASGGGCCGHFISVSDAEIALGGDDQGSRVLKRSSGTALDHSSLILVPSPIDDEEDEDISHGSSNPTIPEDEDGLVPRPSCSGKCLLRAKQAIIATYPLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>D5GQ12</accession>
    <name>D5GQ12_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00012128001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430380">
        <property type="protein sequence ID" value="CAZ86605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842414.1">
        <property type="nucleotide sequence ID" value="XM_002842368.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GQ12"/>
    <dbReference type="GeneID" id="9185183"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012128001"/>
    <dbReference type="HOGENOM" id="CLU_1876959_0_0_1"/>
    <dbReference type="InParanoid" id="D5GQ12"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ86605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="136" mass="14205" checksum="5492305F919A9A51" modified="2010-06-15" version="1">MSDLYGTTSKKSLIAALAIAGVIVFLFLVGLGVMLWCYFRAKPGSQIDALYPRDVESTRTFIPSSTTAIAGWNEKAPSTATGSSEGSLEEGSLEGGRGGGQNVEAPQVAMSRHSSASDTSQNLNGLRPVEPAAIVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5GQ20</accession>
    <name>D5GQ20_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00012155001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430381">
        <property type="protein sequence ID" value="CAZ86613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842422.1">
        <property type="nucleotide sequence ID" value="XM_002842376.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GQ20"/>
    <dbReference type="GeneID" id="9185193"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012155001"/>
    <dbReference type="eggNOG" id="ENOG502RY95">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008869_0_0_1"/>
    <dbReference type="InParanoid" id="D5GQ20"/>
    <dbReference type="OMA" id="HVVHSKI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47842:SF3">
        <property type="entry name" value="DUF676 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47842">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="19"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="146"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="324"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="464"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="328"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="402"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="476"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="527"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ86613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="642" mass="69808" checksum="5EBE9A6260E3ED33" modified="2010-06-15" version="1">MTFKSPPDSVPSGKRKLLLVYIHGFMGNETSFQSFPAHLHNLLSVTLEDLGWGVHTKVYPKFKTRNNISLATEGFSRWLAQFENPGTDVILLGHSMGGILAAEVVLLRDERGGFKHRILGVVTFDSPFLGMHPGVISAGLGSLFRSEGKSPPEESGSGEGGGVYAGENSSMASLSTVHSGSTVDEFFDKRPERNFTIVQSKQEGTWDSALKFITKHHSHLTAATGQYLMSHLEFGGCLADPSGLKNRYNAIRKLETGMQTVDGKTTRVRFINYYTSSTGRSKDPPKPAPAAVHPEHSFDLADAGVEGSGGSELQEMTREMHLGVNPSAPRTHSRNLSTSTISSDGEMTQLDPDPISGSGEFGEAQEKQRGSTPVPAIETTMVGPEPLMLDHALPSIPSYSSPAPPAVSPPPPKPTEFDYSQIPDATVRKAAEKAAAKAMKLWEKSVKDYEKVVKDWEKAIEKNERKKHEKAKKEGKKPASQHDGEVERLRLERERMEREERRLKGIPGPPIYNAAGESTASLKTLPSPSPSLSPTPSPTRSPTPTPETPKKKRRERKFCLTPRTPDACWVKVEMTGVDEVGAHCGLFFLGDVYERLVGDVAGRVEGWVGEEGTRRLLEGEGGADAECGFGGGGWESGVGWVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>D5GQ62</accession>
    <name>D5GQ62_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein YIF1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00012214001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Has a role in transport between endoplasmic reticulum and Golgi.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Endoplasmic reticulum membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Golgi apparatus membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the YIF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430382">
        <property type="protein sequence ID" value="CAZ86655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842464.1">
        <property type="nucleotide sequence ID" value="XM_002842418.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GQ62"/>
    <dbReference type="STRING" id="656061.D5GQ62"/>
    <dbReference type="GeneID" id="9185235"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012214001"/>
    <dbReference type="eggNOG" id="KOG3094">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1708485_0_0_1"/>
    <dbReference type="InParanoid" id="D5GQ62"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005578">
        <property type="entry name" value="Yif1_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14083">
        <property type="entry name" value="YIP1 INTERACTING FACTOR HOMOLOG YIF1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14083:SF0">
        <property type="entry name" value="YIP1D-INTERACTING FACTOR 1, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03878">
        <property type="entry name" value="YIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="125"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU368073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ86655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="164" mass="17891" checksum="110FE514713576E7" modified="2010-06-15" version="1">MQRGNYPPNTAASPPLHHPVPQHHVPIPVMHSPPPLQASSAYGNAYAPAPAVGHPAYGFLNDPTTQMAFQVGGRATEVAGQYVEQNLGKYLQVSALKHYFNVSNGYVLQKLGLVLFPWRHKPWSRSGSQRTGASGQTEGYFLPPREDINSPDMYIPGMPLVVHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4C5</accession>
    <name>D5G4C5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00004048001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835247.1">
        <property type="nucleotide sequence ID" value="XM_002835201.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4C5"/>
    <dbReference type="STRING" id="656061.D5G4C5"/>
    <dbReference type="GeneID" id="9184993"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004048001"/>
    <dbReference type="HOGENOM" id="CLU_328502_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4C5"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="133"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="217"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="548"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="647"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="737"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="846"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="347"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="24"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="49"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="87"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="135"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="549"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="659"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="711"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="740"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="759"/>
            <end position="776"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ79368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="875" mass="95537" checksum="10148C9D0A9B6FEE" modified="2010-06-15" version="1">MTSYPPTPAFGGFPSVLVNTNRKTSANTSNTTGSPTSPATRSPNHFPKATSNTPRLPPSTAQIPCIDPALPGLGKLVERTGGTSVEDREEGELSDIEGDGQGTIINEMSNSIGGNQQAREEQQQIFETYTPRLDGQTQPYSPNMPLSSRDDSYRPRPRGNHALVFNSGYAYQSSAHLPTPPGNGLSYGRIWPADFQYQQIESFEKENLQHKSLLFAEPELSPPTGPQHQPQSHGGATGVPSYSMMPQAFSQLPTEVLLSNPEALRKETETAVSNMLPLKVTFANMVEEGINPRVLRKVFTRLGFPVPVEAASPSPGADVNVTQAAQQDTRQILAPAPPQRTEIERKARTIRKQLEKEKSVRDKERREAEARAAELVFEREAARAEAEKTKRSAEVEAKKEVLRRKIGELKLPAKALSQAPTPVSSMPVVAAIARASMDVPRIPGLSFGESGADISNSPLIQLTNQADEHIDTVMKDSTAPEPKLLPVVSTSAVGLTACTNVAVLSPATPLERPINARKKRPVASDLYSEPQPAKRKFGEQRFGRLIIEVSDDDEGTDEVDDDDAGLGEARTSRSTPTGARPPGPIEHVQRSASTVNVSVALRSVNTAPPGTLNAGYRNGSGGAHAKIALQSKMEEIETLKRAIQEAQHKKKLAAKRGVSTPTSVGTPNNDSVVTPPSDSQSKPARAEVLESIASNGAYLTDKGQQHQFPSAKEKESLKEASQEKLKLLLEELEELEAAEEAEKKKQAESEIAERRRRAEKQEKQKKLEAFEADKVKSRAARQRLREEMERIEKEEMEIEEKKLLLEKELETLEQASGSGFTTVEGSTESINEALRRKTAEIENLKKRMEHTEEGEQALDPSPGPKGPLFSSLRPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4F0</accession>
    <name>D5G4F0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00004079001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835272.1">
        <property type="nucleotide sequence ID" value="XM_002835226.1"/>
    </dbReference>
    <dbReference type="GeneID" id="9185018"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004079001"/>
    <dbReference type="HOGENOM" id="CLU_889007_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4F0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="279"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="313" mass="35096" checksum="645403C923666598" modified="2010-06-15" version="1">MWLPQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLPCLLGGLFVFFCVFIPIRRSQQCRKIPKIFPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4H4</accession>
    <name>D5G4H4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Presequence translocated-associated motor subunit PAM17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00004108001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the PAM complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Mitochondrion inner membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the PAM17 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429987">
        <property type="protein sequence ID" value="CAZ79417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842497.1">
        <property type="nucleotide sequence ID" value="XM_002842451.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4H4"/>
    <dbReference type="FunCoup" id="D5G4H4">
        <property type="interactions" value="46"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G4H4"/>
    <dbReference type="GeneID" id="9184046"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004108001"/>
    <dbReference type="eggNOG" id="ENOG502S1B1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068297_2_0_1"/>
    <dbReference type="InParanoid" id="D5G4H4"/>
    <dbReference type="OMA" id="MIFGFDP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001405">
        <property type="term" value="C:PAM complex, Tim23 associated import motor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013875">
        <property type="entry name" value="Pam17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28021">
        <property type="entry name" value="PRESEQUENCE TRANSLOCATED-ASSOCIATED MOTOR SUBUNIT PAM17, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28021:SF1">
        <property type="entry name" value="PRESEQUENCE TRANSLOCATED-ASSOCIATED MOTOR SUBUNIT PAM17, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08566">
        <property type="entry name" value="Pam17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 12" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="6 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="9 12" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="76"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="113"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="30"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU367146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ79417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="209" mass="23284" checksum="96A2BDF9E23273B6" modified="2010-06-15" version="1">MLTKSVPLRLTSLRISPPFSPATTILLHTHRNASTTTTTTTTSSTSPITTATSSSQSNEKLGWDTFLQLRKQRRRYNLVSSLFTSALGTTAGVGYLANKEIDPTQMIMGMDPIILFGLATVSCGALGWLAGPVLGSSAFSVVKRRIMGQINEREREFLQHIKKNRVDPSFQSFSNPVPDYYGEKIGSIKQYKQWLKDQRAYNRKSKSFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4K0</accession>
    <name>D5G4K0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glutathione hydrolase</fullName>
            <ecNumber evidence="8">2.3.2.2</ecNumber>
            <ecNumber evidence="8">3.4.19.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Gamma-glutamyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Gamma-glutamyltranspeptidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00004188001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Cleaves the gamma-glutamyl peptide bond of glutathione and glutathione conjugates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 8">
            <text>an N-terminal (5-L-glutamyl)-[peptide] + an alpha-amino acid = 5-L-glutamyl amino acid + an N-terminal L-alpha-aminoacyl-[peptide]</text>
            <dbReference type="Rhea" id="RHEA:23904"/>
            <dbReference type="Rhea" id="RHEA-COMP:9780"/>
            <dbReference type="Rhea" id="RHEA-COMP:9795"/>
            <dbReference type="ChEBI" id="CHEBI:77644"/>
            <dbReference type="ChEBI" id="CHEBI:78597"/>
            <dbReference type="ChEBI" id="CHEBI:78599"/>
            <dbReference type="ChEBI" id="CHEBI:78608"/>
            <dbReference type="EC" id="2.3.2.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>an S-substituted glutathione + H2O = an S-substituted L-cysteinylglycine + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:59468"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="ChEBI" id="CHEBI:143103"/>
            <dbReference type="EC" id="3.4.19.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 8">
            <text>glutathione + H2O = L-cysteinylglycine + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:28807"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:61694"/>
            <dbReference type="EC" id="3.4.19.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 8">Sulfur metabolism; glutathione metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the gamma-glutamyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.2" evidence="8"/>
    <dbReference type="EC" id="3.4.19.13" evidence="8"/>
    <dbReference type="EMBL" id="FN429988">
        <property type="protein sequence ID" value="CAZ79443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835286.1">
        <property type="nucleotide sequence ID" value="XM_002835240.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4K0"/>
    <dbReference type="FunCoup" id="D5G4K0">
        <property type="interactions" value="146"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G4K0"/>
    <dbReference type="MEROPS" id="T03.011"/>
    <dbReference type="GeneID" id="9188397"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004188001"/>
    <dbReference type="eggNOG" id="KOG2410">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014813_4_0_1"/>
    <dbReference type="InParanoid" id="D5G4K0"/>
    <dbReference type="OMA" id="ICGMGPP"/>
    <dbReference type="UniPathway" id="UPA00204"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036374">
        <property type="term" value="F:glutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103068">
        <property type="term" value="F:leukotriene C4 gamma-glutamyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006751">
        <property type="term" value="P:glutathione catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006805">
        <property type="term" value="P:xenobiotic metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.40:FF:000001">
        <property type="entry name" value="Gamma-glutamyltranspeptidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043138">
        <property type="entry name" value="GGT_lsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000101">
        <property type="entry name" value="GGT_peptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043137">
        <property type="entry name" value="GGT_ssub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00066">
        <property type="entry name" value="g_glut_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11686">
        <property type="entry name" value="GAMMA GLUTAMYL TRANSPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11686:SF9">
        <property type="entry name" value="RE13973P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01019">
        <property type="entry name" value="G_glu_transpept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01210">
        <property type="entry name" value="GGTRANSPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="71"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="35"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="463"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="481"/>
            <end position="483"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="505"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="533"/>
            <end position="534"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="556"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR600101-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR600101-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU368068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ79443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="657" mass="71589" checksum="4F180ACF28597E9C" modified="2010-06-15" version="1">MPPNKVRFSPTVSRSHTPPHSPSPTPSSSSILYNTDDDQDSDDDDSVRRNLLPTIARPLVRRRRRLGKRNAWMKFGMTVLLAAALLGLGVLFGVIFWENEEGIVDDGILVDAPRGAVSSDVLTCSRIGVELLKKGGNAIDAAIGTAACIGVVNSFASGIGGGGFMVVREGDGRSKAYNFRETAPGSASRDMYSGDPLKARVGGLAVAIPGEVHGYATAHARSGRLPWQEIWQPSIDLARRGFHIPPMLAGIMAKEAPFFRQHREEWEFLFSQETGELLQVGELMKREAYARTLEVIAGKGEYARGGVYSGVKQFYNGSIAERIVEAANKHGGILTTEDFSRYFTIVEDTVRTHFHGREVITCPPPCSGAVLIEGLNIAEKLDISDPDKSLSYHYLVEIMKWISAGRTELGDPADPVVASNSKRIHQLQSKSYAEAVRANISADTTYSWEHYNPAYEPVDPKGTSHLSVLDNYGNAVALTTTVNLYWGAKVHDPQTGIVLNSEMDDFSIPGRSNAYRLEPSIYNYIIPYKRPLSSTTPTIIVEEDGAPGLVIGGSGGSRIVTGVFQAIVKNYLWGYNLLDTIKSPRLHHQLIPEVVSAERGTEKWILEGLEERGHVIGLRGIGEASGGSVIQVVKGMDSGRVEAVADWWRKGGRAAGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4L3</accession>
    <name>D5G4L3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00004209001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="FN429988">
        <property type="protein sequence ID" value="CAZ79456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835299.1">
        <property type="nucleotide sequence ID" value="XM_002835253.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4L3"/>
    <dbReference type="STRING" id="656061.D5G4L3"/>
    <dbReference type="GeneID" id="9188392"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004209001"/>
    <dbReference type="eggNOG" id="KOG4231">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_794773_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4L3"/>
    <dbReference type="OMA" id="WTIANEM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047499">
        <property type="term" value="F:calcium-independent phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019369">
        <property type="term" value="P:arachidonate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046486">
        <property type="term" value="P:glycerolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07199">
        <property type="entry name" value="Pat17_PNPLA8_PNPLA9_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24185">
        <property type="entry name" value="CALCIUM-INDEPENDENT PHOSPHOLIPASE A2-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24185:SF1">
        <property type="entry name" value="CALCIUM-INDEPENDENT PHOSPHOLIPASE A2-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 4" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3 4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="5">
        <location>
            <begin position="15"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="4">
        <location>
            <begin position="53"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="4">
        <location>
            <begin position="214"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ79456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="389" mass="43125" checksum="F1EBA49E91A43C6B" modified="2010-06-15" version="1">MSDIGLLSTCQLLTFSLSRGGVRSIVQLTILQELEISIGLEVNIVDFFDLIIGTSGGGIVAAGLGIRKFGVQKCIDKFQQVSKQAFTKRTGAGGVFGPLIEAQHHSRYKTTNLSEALQKAFGEDTILFGGQRDPTHEPITRVGVSAATPSRHTFLLSNYQRPQVASVGRPTYTFYRGETAEEELKVWEALRATSAAPRYFKSFYHKASGYTFYDGAITCNNPIFLAHTERKILWPEQHERDPDILLSIGTLWSNQDGSSSEIDKANPKGKFGVRQYVKNLFGIMMAAVHDDLDCEKTWSSFYDSLNISEGDGHRKKKYWRINPKIPNTPPALDSVSSMDDLQDIARKYCSTSPEIKNVASTLIASLFYFQMKSASKITLSGFQTCEGKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4S6</accession>
    <name>D5G4S6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00000080001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429990">
        <property type="protein sequence ID" value="CAZ79512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835355.1">
        <property type="nucleotide sequence ID" value="XM_002835309.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4S6"/>
    <dbReference type="GeneID" id="9188448"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000080001"/>
    <dbReference type="eggNOG" id="ENOG502SARD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096567_3_0_1"/>
    <dbReference type="InParanoid" id="D5G4S6"/>
    <dbReference type="OMA" id="CIRFLQF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42083">
        <property type="entry name" value="MARVEL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42083:SF1">
        <property type="entry name" value="MARVEL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="163" mass="18306" checksum="A6418061E4A386D6" modified="2010-06-15" version="1">MVQKNKSHRAPLPDSRKITIPRLILRILQILISLAASGIYGRDLQSATKNGERADSRWVFAEVVVGVSIVLAVVYILPFVRSFRTFYLDGIVFLFWVILLGIFGMLFGRVDCRGDGDCRRMKGGIWVDVVGVLLWFATFVGGGVMWWKDRHGRSIYTGRGSVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4V2</accession>
    <name>D5G4V2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00000098001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429992">
        <property type="protein sequence ID" value="CAZ79545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835388.1">
        <property type="nucleotide sequence ID" value="XM_002835342.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4V2"/>
    <dbReference type="GeneID" id="9183027"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000098001"/>
    <dbReference type="HOGENOM" id="CLU_2833015_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4V2"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ79545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="66" mass="7798" checksum="1A05B01330B84C66" modified="2010-06-15" version="1">MYEYGSVLIVIFVYARGKLLRGTMSDIEICFRVQRTFTLVLRYTVIRVVVRFVYRVILGSDKVTHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5G594</accession>
    <name>D5G594_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">PAN2-PAN3 deadenylation complex subunit PAN3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">PAB1P-dependent poly(A)-specific ribonuclease</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Poly(A)-nuclease deadenylation complex subunit 3</fullName>
            <shortName evidence="8">PAN deadenylation complex subunit 3</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">PAN3</name>
        <name evidence="10" type="ORF">GSTUM_00004233001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Regulatory subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein PAB1. PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1. May also be involved in post-transcriptional maturation of mRNA poly(A) tails. PAN3 acts as a positive regulator for PAN activity, recruiting the catalytic subunit PAN2 to mRNA via its interaction with RNA and with PAB1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer. Forms a heterotrimer with a catalytic subunit PAN2 to form the poly(A)-nuclease (PAN) deadenylation complex. Interacts (via PAM-2 motif) with poly(A)-binding protein PAB1 (via PABC domain), conferring substrate specificity of the enzyme complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">Contains a pseudokinase domain. The protein kinase domain is predicted to be catalytically inactive because some of the residues important for catalytic activity are substituted and it lacks the equivalent of the binding site for a peptide substrate. However, it has retained an ATP-binding site and ATP-binding is required for mRNA degradation, stimulating the activity of the PAN2 nuclease in vitro. The nucleotide-binding site is juxtaposed to the RNase active site of PAN2 in the complex and may actually bind nucleosides of a poly(A) RNA rather than ATP, feeding the poly(A)-tail to the active site of the deadenylase and thus increasing the efficiency with which this distributive enzyme degrades oligo(A) RNAs.</text>
    </comment>
    <comment type="domain">
        <text evidence="8">The N-terminal zinc finger binds to poly(A) RNA.</text>
    </comment>
    <comment type="domain">
        <text evidence="8">The pseudokinase domain, the coiled-coil (CC), and C-terminal knob domain (CK) form a structural unit (PKC) that forms an extensive high-affinity interaction surface for PAN2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily. PAN3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835530.1">
        <property type="nucleotide sequence ID" value="XM_002835484.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G594"/>
    <dbReference type="FunCoup" id="D5G594">
        <property type="interactions" value="608"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G594"/>
    <dbReference type="GeneID" id="9188106"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004233001"/>
    <dbReference type="eggNOG" id="KOG3741">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016423_0_1_1"/>
    <dbReference type="InParanoid" id="D5G594"/>
    <dbReference type="OMA" id="YVFHSVD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031251">
        <property type="term" value="C:PAN complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.3700:FF:000001">
        <property type="entry name" value="PAN2-PAN3 deadenylation complex subunit PAN3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PAN2-PAN3 deadenylation complex subunit PAN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.5160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03181">
        <property type="entry name" value="PAN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030844">
        <property type="entry name" value="PAN3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041332">
        <property type="entry name" value="Pan3_CK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12272">
        <property type="entry name" value="DEADENYLATION COMPLEX SUBUNIT PAN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12272:SF11">
        <property type="entry name" value="PAN2-PAN3 DEADENYLATION COMPLEX SUBUNIT PAN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18101">
        <property type="entry name" value="Pan3_CK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 8" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="133"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Knob domain" evidence="8">
        <location>
            <begin position="418"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="379"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="214"/>
            <end position="221"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="279"/>
            <end position="280"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ79687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="522" mass="59806" checksum="D0F4BA2ED1426EB3" modified="2010-06-15" version="1">MAPAIDPQLYHHQGLQHGFENMGLGENTTANAAMNAYDFTVGASVNPYAQTGVDMFYQPQQTYQHPLNYHLYAPLAPYPQKLQPFQKNIHGFFLPDNLREELQKKADATLQTLPAISSILPPSIEEYHQLVPLDISNQKSAQVFGYPSWAYKAFSSEDGNAYALRRLEGFRLSNEADIRIVQRWRKVLSANIVTIHDAWTTTKFGDSSIVFVYDYHPLSKTLAETHFGPNPRYPNPTRQHQPTGPIVPEQVLWSYIVQIASALRVIHSAGLAARVIEPSKILLTNKMRIRLNCCGIFDVIHPETTRSQESVRECQQEDLIQLGRLMLSIAFNTPSAIQYPQKSMEYIGRHYSIQLRDSIYYLYTGSTKNIDEYVKSIANQAFQNFDSALHYEDSLESALSRELENGRLVRLLCKFGFINERPEYDHDPRWSETGDRYYLKLFRDYVFHSVDESNNPVVDMAHVLTCLNKLDAGVDEKIMLVSRDSMNCFVVTYKEARPQQVQTMKFDLLLATYLRSFMVPSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>D5G598</accession>
    <name>D5G598_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00004237001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835534.1">
        <property type="nucleotide sequence ID" value="XM_002835488.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G598"/>
    <dbReference type="STRING" id="656061.D5G598"/>
    <dbReference type="GeneID" id="9188110"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004237001"/>
    <dbReference type="eggNOG" id="ENOG502SG4F">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_309091_0_0_1"/>
    <dbReference type="InParanoid" id="D5G598"/>
    <dbReference type="OMA" id="RLCHNDG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="159"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="199"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="452"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="517"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="871"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="517"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="573"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="601"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="632"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="656"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="709"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="741"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="790"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="809"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="916"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="936"/>
            <end position="945"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="954" mass="105389" checksum="E86DC99C339FF649" modified="2010-06-15" version="1">MSLVVRGGISISIFDRRTAPLTPFSDLSDNPDTPGTTYSFQDPPQHKSANMQADPAHHPLPGLLDHHVLPATDDEMMDSDTDIRKDSDFDIEIDADEVYSNAGGADLDANYRMEEDNADNSHQLYDNDDIMLDGEDDIIFEKQQVDEAGPVPDGIPTIPGASPKTLPEEQSTVPEEPSITPNIPPAISEQADQPFLPLEDPSAGEAAHLTDSQQTVSPAGESRGTLALLENTEKTVAHEAVTQDGDLQHKDNVWSENTSVTLANEVVSLEERQANEATEQPHQSVTTGNEVEEQGAVNLANEPPGFDPHPVVVQYEQSHVSLFPPPDDVDLPFFIKDVGVCLRGMNELFSELRNVLDGSVSPGDELVIKIVDYGVEISEVSFFLHLSSYEFSLQHILDLHVRLSLQDNIENPEALRLVLFSRPSVSDRINMLLSSANEGLGYNKAIEYARRKSGDNDANSEQRFVETSEGGNLQEPEVEDYNNNDDSSVAFAHATDKPTEVEPNLTEDQGSILEQKEEFEEDDGDDDDDDDDFDLEYGNDDIANGVEGEGNEGGDDQKDEAPLHPTEAVSKDSPASINESPNYGSETPRAQENAVLELETVSDSASPRDTQTPDEVEESVSTVQGDEAHSTEEEEELIEYEDYETFGESESHHSPHTVNSESGEQNLGKNEPPPHEDPEQQQDAAGPSDTPELYEQESAVNFIDPGENAADRNHNESHSPSDGDVNVNNETSSIPVFGGDGEGEEDYVGEQQEEYGEYEYDGQQGFEAGQDASRDYIPDDEQYDIQDPLENNHESNMQRHSRPEQNDQPTDDEQNQYLDETVEYEDGYEHYDAEYDGAVNGQEALSGETEYHVGYQIYDLNQAHLRTDYGDDTVTGDANESGVAINAENDEAVDSLHSPEPAPEVESLLGYDSDDAEHNIFLEAKPSTEQKRMYDELEQDEDDDEILAKRQRSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5B5</accession>
    <name>D5G5B5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00004258001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835551.1">
        <property type="nucleotide sequence ID" value="XM_002835505.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5B5"/>
    <dbReference type="GeneID" id="9188127"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004258001"/>
    <dbReference type="HOGENOM" id="CLU_1564024_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5B5"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ79708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="171" mass="19492" checksum="70FE4A976C7E5A9E" modified="2010-06-15" version="1">MTNRPQPAVYLVSGSGPTTVAIAQIELRKVFTFHAVWKWILSKQPQYIGKPMVIENRFFVETEIVADDQQLPIGSTGFHRIFDDASWDDCKIDLLAHNIPDFPGAANRKPIFSVRVLQPDVDVFDFQNREIVFYSVWKLLNIISSPPPDEEMLQPRGHLLAYLQALGERGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5E3</accession>
    <name>D5G5E3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00004293001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835579.1">
        <property type="nucleotide sequence ID" value="XM_002835533.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5E3"/>
    <dbReference type="STRING" id="656061.D5G5E3"/>
    <dbReference type="GeneID" id="9188155"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004293001"/>
    <dbReference type="eggNOG" id="KOG1465">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1866590_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5E3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005851">
        <property type="term" value="C:eukaryotic translation initiation factor 2B complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051855">
        <property type="entry name" value="eIF2B_beta_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45859">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45859:SF1">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ79736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="137" mass="15118" checksum="253CE05F9A89539F" modified="2010-06-15" version="1">MASQMVTLRTPDLQWWLDHLDTAFAPDVSVDLFVGVLKRRSVKGPEAAAIATAQLFLRLIYAHPFSSIGDLVDHISSIGTKLSKAVPRELAVRNMARRVIGIIREEAENNGMGDLFQAALEIGTPPGFSCSYKECRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5S8</accession>
    <name>D5G5S8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00001463001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430001">
        <property type="protein sequence ID" value="CAZ79871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835714.1">
        <property type="nucleotide sequence ID" value="XM_002835668.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5S8"/>
    <dbReference type="GeneID" id="9187549"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001463001"/>
    <dbReference type="eggNOG" id="ENOG502SFC0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115534_2_0_1"/>
    <dbReference type="InParanoid" id="D5G5S8"/>
    <dbReference type="OMA" id="WPPDFSL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="115"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ79871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="126" mass="14698" checksum="609F18EFB48E58B5" modified="2010-06-15" version="1">MFPTRALLTQKVYKAKKPWPPDFSLLSQKHQFRLERRYRRRMKLKFARPRLHKAVNLAQGVIISAVLLWGVFINDWGERGNRPVQPIRNWVSDLRKSFWSTPSRPEVSKQPRTPSSSGSISEASGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5G721</accession>
    <name>D5G721_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00004564001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430020">
        <property type="protein sequence ID" value="CAZ80314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836123.1">
        <property type="nucleotide sequence ID" value="XM_002836077.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G721"/>
    <dbReference type="GeneID" id="9182505"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004564001"/>
    <dbReference type="HOGENOM" id="CLU_2943497_0_0_1"/>
    <dbReference type="InParanoid" id="D5G721"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ80314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="60" mass="6726" checksum="2D129DAF5D040BFB" modified="2010-06-15" version="1">MTGIPIKMYHESLFALRLLSFLFRIESTVSARMRRYMMIGSGVETDSGVAGSTKNKLRGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7H2</accession>
    <name>D5G7H2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00002462001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430027">
        <property type="protein sequence ID" value="CAZ80465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836274.1">
        <property type="nucleotide sequence ID" value="XM_002836228.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7H2"/>
    <dbReference type="STRING" id="656061.D5G7H2"/>
    <dbReference type="GeneID" id="9188490"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002462001"/>
    <dbReference type="eggNOG" id="KOG0272">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_57_4_1"/>
    <dbReference type="InParanoid" id="D5G7H2"/>
    <dbReference type="OMA" id="TIRTWPL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001196">
        <property type="entry name" value="WD repeat protein (AFU_orthologue AFUA_1G12380)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_b-propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848:SF8">
        <property type="entry name" value="F-BOX AND WD REPEAT DOMAIN CONTAINING 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="77"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="119"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="266"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="308"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="355"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="373"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ80465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="389" mass="43063" checksum="C866FD1E34972453" modified="2010-06-15" version="1">MSMSNDPGHFFQTETALANAKRRSAKSSNKNGSPIPLASKLLSIIPDLTSSMRVYIAESGGITRRVDLESGDKSCTYKGHTAPVTSLALNTAQGILFTGSWDKTIITYSTETRQKLRTFAGHSDFVKSLLFVPSPKHEGGLLFSGSSDASIVIWDAGTGQRLNTLKGHNRAVRALAVDPITSNSEAAIIYSGGSERDIKRWEIPYANVSATTESSETIMEHDTSVNMIRFQGEDAEMWTASADNTARKIDIRTETKKNGTCRSEAVLRHPDYVNDVVVEPRGRWIITACRDEAVRIWDISTGELYHIYDGHIEEVTGLAIVGNGEMVASISIDNTVRTWSLRPVDLMKAVEDKRVQEQGMEEEPVQERKEGFLTEEEERELAELMDDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7H4</accession>
    <name>D5G7H4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GTP-binding protein ypt1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00002466001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Preautophagosomal structure membrane</location>
            <topology evidence="8">Lipid-anchor</topology>
            <orientation evidence="8">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430027">
        <property type="protein sequence ID" value="CAZ80467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836276.1">
        <property type="nucleotide sequence ID" value="XM_002836230.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7H4"/>
    <dbReference type="FunCoup" id="D5G7H4">
        <property type="interactions" value="1069"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G7H4"/>
    <dbReference type="GeneID" id="9188492"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002466001"/>
    <dbReference type="eggNOG" id="KOG0084">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_23_1_1"/>
    <dbReference type="InParanoid" id="D5G7H4"/>
    <dbReference type="OMA" id="TQMAKDF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01869">
        <property type="entry name" value="Rab1_Ypt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000069">
        <property type="entry name" value="Ras GTP-binding protein YPT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057289">
        <property type="entry name" value="Rab1/Ypt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0636">Prenylation</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00060489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00074496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ80467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="201" mass="22299" checksum="4E9ACF79FD44BBAA" modified="2010-06-15" version="1">MNPEYDYLFKLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTVELEAKTVKLQIWDTAGQERFRTITSSYYRGAHGICVVFDVTDMDSFNNVKQWLQEIDRYATEGVNKLLVGNKSDIADKKVVEYTVAKEFADSLGIPFLETSAKNATNVEQAFLTMARQIKERMGSTTVNNKPTVSVQQGTGVQQGSAGGCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7H8</accession>
    <name>D5G7H8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00002472001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430027">
        <property type="protein sequence ID" value="CAZ80471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836280.1">
        <property type="nucleotide sequence ID" value="XM_002836234.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7H8"/>
    <dbReference type="STRING" id="656061.D5G7H8"/>
    <dbReference type="GeneID" id="9188496"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002472001"/>
    <dbReference type="eggNOG" id="ENOG502QV85">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_316914_0_0_1"/>
    <dbReference type="InParanoid" id="D5G7H8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018562">
        <property type="entry name" value="ARS-binding_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42048">
        <property type="entry name" value="ARS-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42048:SF1">
        <property type="entry name" value="ARS-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09441">
        <property type="entry name" value="Abp2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="399"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="518"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="664"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="825"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="859"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="406"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="442"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="531"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="615"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="664"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="874"/>
            <end position="885"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="920" mass="100663" checksum="D1DF7D64AD928404" modified="2010-06-15" version="1">MAGPSPHVRNSQNPSLQDAVNPRCTSTQTLTSALFTTRLPAPSLAASLKVTKIPLIEDDSAPRVVVPRLLVLAESTPDEGVQTLSQYTHLDDGMDAEHYQHSLDEEEDDDEESSFVQGITAGGHDHSQDTSPPEFSHQSFERQHLSIDPALDTPPPENHPHHLHHHHHAGQDNDDEEGDGSSHHRLQQPQQQVQNHFVQSQLPRQLYTDLARSANQFSLQHHAPDQLSTPPAQFQASSPASGKILPTFTPSDRSLPDRAVTDETLDDAYVAFILYCNPSVPLDCDTAELRKAFRQPPKSDGKTFNIHHLLQLIEKFEEKEIKTWTKLAIQLGVERTADQSAQKVQQYAVRLKRWMHAMHIDAFFEYILNKPHAYYQHIPHSLPTSDSLDQMRDGVPIEEDLALRALHPETRPKRGRRKTDDKDDGSEKDMPDSKRPHIGIETPTTTDPTSAMEHFSNSLFPPHPQSAAPSSAGGDGMERFLEESDPWAAATQSVLSGGGNSATPTGGQQFRWRAFPREGTTPQSAHAPPTILTSHDNITPQDEIQTPVSTTPVSASKPRSRRRHGPAVSSAWPSSGNPLTGKLRGRPPSNRSVRDGPFSTFPAHPTGKGHTIDLSSPGSITPTPTSTPVTTHAPQQFTFRPEQLHLTVPARVGGNAISMASPASAITPSPHMNGNSWKREKSPLGRESANIYEAQGLEDVERRFSVSLLQAQCHDGLVLGVEDAKRIAEKIVSALKRAWPNSGGRVDDKIVAALLGCGDDGVFREVKISRLSGGSDRRDLDDLGLDVDDGDQLNGREGGERGFDVRWSIQFGSVKGEFSQIVSVGGNSGLDHNNDPPPSSLSQIGEDNDRVLKELEGLEDFGGGVNDDPHEKSTGSSNGASNTGNVEKLRKRVLELERSLKAKEKEIHGIKENVLKAVVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7Z3</accession>
    <name>D5G7Z3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00002741001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat RAPTOR family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430035">
        <property type="protein sequence ID" value="CAZ80636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836445.1">
        <property type="nucleotide sequence ID" value="XM_002836399.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7Z3"/>
    <dbReference type="FunCoup" id="D5G7Z3">
        <property type="interactions" value="767"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G7Z3"/>
    <dbReference type="GeneID" id="9184109"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002741001"/>
    <dbReference type="eggNOG" id="KOG1517">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001136_3_0_1"/>
    <dbReference type="InParanoid" id="D5G7Z3"/>
    <dbReference type="OMA" id="TEVCTND"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031931">
        <property type="term" value="C:TORC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071230">
        <property type="term" value="P:cellular response to amino acid stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030307">
        <property type="term" value="P:positive regulation of cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031929">
        <property type="term" value="P:TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004083">
        <property type="entry name" value="Raptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12848">
        <property type="entry name" value="REGULATORY-ASSOCIATED PROTEIN OF MTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12848:SF16">
        <property type="entry name" value="REGULATORY-ASSOCIATED PROTEIN OF MTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01547">
        <property type="entry name" value="YEAST176DUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="2">
        <location>
            <begin position="948"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="563"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="580"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="619"/>
            <end position="628"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ80636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1040" mass="116029" checksum="34C4F445B3A4AE7A" modified="2010-06-15" version="1">MELRAKDPNALVTTSYSDCIQLAACGSKETLPMNPDLPADLFTCCLTTPIEIAIRFFVLQNPLPSNLTVEQAMKIPGRLQERRTPLGELNWIFTAITDTIAWNTLPRPLFKKLFRQDLMVAALFRNFLLSQRIMRVHQCHPISSPPLPETHHHPLWQSWDLAVEMVLAQLPALNRATDGGPAYEYQHSGFFTEQLTAFEVYLSQGAIDRKPPDQLPIVLQVLLSQVHRLRALILLSKFLDLGPWAVNLALSIGIFPYVLKLLQSAAQELKPVMVFIWARILAVDASCQADLLKDNGYSYFIQILSPASGIPVGNASEHRAMCAFILAMFCRGFHQGQVVCMPPNVMGACLAHLGDENPLLRQWACLCLSQLWHDFAEAKWQGIREGAHLRLCELATDNVPEVRAAVLYALTSFLGIPEMSEEVAKIEQDVAGSLLIMTADGSNMVRKELVIFLSMFVKRNEGKFLVAAFEQMVQERDDLTANGSAKKKDTLGAISPNTIFSAIWQALLMFSVDPFPEIGQAAQIVVDYVHLALLQSPLGQYAQDVIEEISRANLRSFNLTLPTNSMPESKRPMALGYASGETTPTNSAPATPKTLKGSWLANGSAERSPEWNRPPDANDPQSSSTSYRTAKEPITRNFHAKGVDKIPSIPLKSQFMEWSIEYFREPQMKPNEADEPGSVDYNQRLWRRTRNDRIIIDTQTQKERASASRWDHNKGVFNNGSLPMRLLFHQFESHLIAVDEKDSVSVWDWAKGVKTNHFSNGNPTGTKITEIKFLNEDDVALLMTGSGEGVVRIYRNYENDGEVELVSSWRALTDLLPSRRSSGLVAEWQQGRGTMLVGGDVRVIRVWDAPREMCMSDIPARSGSCITSLTSEQVAGNIFVAGFGDGAVRIYDRRLAPRDSMVKVWKQHKAWIVKAHMQRGGMRELVSGSATGEVKLWDIRMDNPVRGFLAHTKGMRSLSVHEHAPIIATGSTWHDVKLWNTSTPKDLPLSTIKPYSSFLHQNRSNPVTGLAFHPHRMMVACSGAGDNHISLYNCDSKGGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8J5</accession>
    <name>D5G8J5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00002870001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430046">
        <property type="protein sequence ID" value="CAZ80817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836626.1">
        <property type="nucleotide sequence ID" value="XM_002836580.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8J5"/>
    <dbReference type="STRING" id="656061.D5G8J5"/>
    <dbReference type="GeneID" id="9186062"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002870001"/>
    <dbReference type="eggNOG" id="KOG0725">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_1_1"/>
    <dbReference type="InParanoid" id="D5G8J5"/>
    <dbReference type="OMA" id="NSLACGP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052178">
        <property type="entry name" value="Sec_Metab_Biosynth_SDR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43618">
        <property type="entry name" value="7-ALPHA-HYDROXYSTEROID DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43618:SF17">
        <property type="entry name" value="RHAMNOLIPIDS BIOSYNTHESIS 3-OXOACYL-[ACYL-CARRIER-PROTEIN] REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ80817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="263" mass="27748" checksum="58CDDBE5811EBE31" modified="2010-06-15" version="1">MDVSTLFSVKDKVVLVTGGAKGIGRMISEGFVANGAKVYISSRDAKSCESAAAELTTMGPGTAHAIPADFYKLSDCQNLVAELKKRETKLHVLVNNSGSNWGESYDRYPDAAWTRVLTLNLHRVFTITQLCTPLLEAAAGIVGDPGRVIHIGSIDGIRVPALETFAYSASKAGLHHLSEVLANHLGKRNITSNSIACGPFESKMMAATLKKFKPMIEGSIPLKRIGTPQDVAGTCLFLASRAGAYVNGATITVDGGSAIGAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8Q2</accession>
    <name>D5G8Q2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00003084001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430050">
        <property type="protein sequence ID" value="CAZ80895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8Q2"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003084001"/>
    <dbReference type="HOGENOM" id="CLU_2795781_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8Q2"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ80895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="68" mass="7458" checksum="6E46C412CD5CCE1B" modified="2010-06-15" version="1">MVCEILPDLSIPSNALATGLLLRLCTTRHHPKSFATSRLGLVGASFGKKRISDRWPRLCFTSATPFIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8Y1</accession>
    <name>D5G8Y1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RecQ-mediated genome instability protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00004892001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RMI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430053">
        <property type="protein sequence ID" value="CAZ80974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836783.1">
        <property type="nucleotide sequence ID" value="XM_002836737.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8Y1"/>
    <dbReference type="STRING" id="656061.D5G8Y1"/>
    <dbReference type="GeneID" id="9181257"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004892001"/>
    <dbReference type="eggNOG" id="KOG3683">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1939647_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8Y1"/>
    <dbReference type="OMA" id="KVEAWHD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016604">
        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031422">
        <property type="term" value="C:RecQ family helicase-topoisomerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.770">
        <property type="entry name" value="RecQ-mediated genome instability protein Rmi1, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042470">
        <property type="entry name" value="RMI1_N_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013894">
        <property type="entry name" value="RMI1_OB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14790:SF15">
        <property type="entry name" value="RECQ-MEDIATED GENOME INSTABILITY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14790">
        <property type="entry name" value="RECQ-MEDIATED GENOME INSTABILITY PROTEIN 1 RMI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08585">
        <property type="entry name" value="RMI1_N_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecQ mediated genome instability protein 1 OB-fold" evidence="3">
        <location>
            <begin position="3"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ80974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="130" mass="13762" checksum="97F22489C3CB1990" modified="2010-06-15" version="1">MGERGEFMKGKEVIRLVPEEEGDEGGEGGPVAGRPKVVVAGTGPHKLLMEDAGGRGVYGIELQSVQGIQVDMPMGAKMVLKNVTVARGILLLTPANTLVLGGKVEALNQQYLANRKQVLNAALEKLRSQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>D5G963</accession>
    <name>D5G963_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00003160001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TBP family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430055">
        <property type="protein sequence ID" value="CAZ81056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836865.1">
        <property type="nucleotide sequence ID" value="XM_002836819.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G963"/>
    <dbReference type="FunCoup" id="D5G963">
        <property type="interactions" value="1032"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G963"/>
    <dbReference type="GeneID" id="9184342"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003160001"/>
    <dbReference type="eggNOG" id="KOG3302">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060161_1_0_1"/>
    <dbReference type="InParanoid" id="D5G963"/>
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</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>D5G973</accession>
    <name>D5G973_TUBMM</name>
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        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
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        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
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    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ81066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1821" mass="200106" checksum="02187FB6826820D4" modified="2010-06-15" version="1">MSEPYRSHSASGGSGGGRGYVGNNSRGAAQQNYLRSRTPSFDNGDDATYFDGGTGVGARQSIRGPEELFYDRAYSTPHDPSRRGSVGPISTSAVGRTQSQASSGGYFAVTPGGQYSPAFSNTSYDTAPSSAPYPTTPGSGYNGSQHQPYNPAAYQAQPQRHATSAGFGVSPEYQPQRHATTAGVGNYNSGQFSPTTATYNPSTFNTASYPTGTYRTGAVPQTAPLQSHPSQSFGGGPGYSQYATRPQYATTSSLYGSGQPYSPGLLPPPPAQSAPPQSTYQPPPPPPPPHPPGSPGFAPGSYGQPLFGSTAYQHSSLSHQTSVSQGNKSYLTRPETHYNSGSDSDISGPDQVGHPLPIPPKDRPEPLQNTGYNVAGTNPLPSPPPPTPPSHGAFRQGHSVEDSRHPQARPLPHAPHGVHDKGRQRKEQEAAEELYADVEASLAEMENKPQQAPGRRPVPMNDQHEEGPAPLFGGAGRVPYSQNGSGPTNGHLTTAQTRYGYYSGESDAEAAEGLAMMRLAEVQEERERRAMEDPSTQTPPNKTTEDVESDSDFPPGVDMTMYGGFPGMSGFYGADSYGGNNDIPVLPTYKSVPPPPPARGNDMGRTSSAAGSSSTAPRLPEYSQFAIPNDMDVHPFPQFQNARVDTWGTGGLAEPSSQRRPMSYDDGDEDVDRSGSQSPSKLQNDSDDDLPELFYHPPAGGVYGSPVNTRPLPSPPYNDLMATGSLPYPEDLPPYQQQFPVAGRVQTMRPYATPTSAEVIGPTMPVLGPGPQFPRSTSMSSHTSTPPTIPPIRSKTDGKAHPARGHRQSALPTGAQTLAVNAEELTLNDLPPIPLTKKFDPKKLSSRDFRNCPTPWALSAIQAWLKEMTEGEQDLKQPAVEEGIVALFTHYVPTMNVADAEALAVRVVSGMIKERALVIDEEWVKFGEGEVSGVIFQITGNGCYAPKLHEIECPGRCYAHHCARTLRKITLSQHPDEFDKKKEDWATFWKIKKEDIADVSKKEIERQNNLHEIVQTEDEYMEHMRVLKALYRDPIANTQPPIIKPTRLDGFVKDVFGKADAVRKVSEEHLLPQLKFRQREQGPWIVGFSDIFREWIRKAKTAYLEYAAAFPKADMLVRREAERNLLFRQFLEQCRQDPRSRRLDWVTFLKAPITRLQRYSLLLFTVLKHTVIEGEEKEILQKAIEEIKAVTLECDARVDEMSKRVALWELDTRLMMRQWDVDLRLDEKGRELIFKGDLQRNGNNRFTWLETHAILFDHYLVLAKTTMQKDAHGGAKHGRYDVSRMPIPMDLLVLESTNDEPVVRSTANRLGIGGPTAAAKDKNEQKSGRHNTIAATPSPGALTHSNTGLSTTSTNTAPGRLVTALPDRSAGDDRVMYPFRIKHLGNRSFKADDNTYILYAPTQQNRMDWCEKIIQAKERHAQSLHAQNAEPFRLKVMADTAFGYETTAQSGPKPIIVRGTPLDRAIGEVEKMYEKAPGPRPGVICRASVNCATAFTVNYGREMVAIGTDYGVFTADAKNPRGWQRSINLPKVTQIAVLEDFSLFLVLSDKSLIAYHLDVVIPPPGASPDAAVKQRPPQKLSGGKDVGFFATGRMKERSLVFYKKREGLSSTFKVLEPVFQKAPEKKSRFSRKGTTGFFQEFDEFYIPTDCYGINLFHSSLAIQTSKGFEVMTLDKKQAWSVPELKASHVTLIASRLQGQKPLGMFRLSDIEFLLCYEECAVYVNKHGDISRSVIMEFVGKAKSVAIYGPYILIFDNDFVEIRNADNGRLRQVIAGRDCRCIDDAQCGGSAGTRTIKVAMAHPEVEGRQLVVELLVNEGQED</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5G976</accession>
    <name>D5G976_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00003183001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the OXA1/ALB3/YidC family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430056">
        <property type="protein sequence ID" value="CAZ81069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836878.1">
        <property type="nucleotide sequence ID" value="XM_002836832.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G976"/>
    <dbReference type="FunCoup" id="D5G976">
        <property type="interactions" value="55"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G976"/>
    <dbReference type="GeneID" id="9184355"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003183001"/>
    <dbReference type="eggNOG" id="KOG1239">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029282_1_2_1"/>
    <dbReference type="InParanoid" id="D5G976"/>
    <dbReference type="OMA" id="WQRKRIV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032977">
        <property type="term" value="F:membrane insertase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032979">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane from matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20069">
        <property type="entry name" value="5TM_Oxa1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001708">
        <property type="entry name" value="YidC/ALB3/OXA1/COX18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028055">
        <property type="entry name" value="YidC/Oxa/ALB_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428:SF65">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX18, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428">
        <property type="entry name" value="OXA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02096">
        <property type="entry name" value="60KD_IMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="205"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="243"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="Membrane insertase YidC/Oxa/ALB C-terminal" evidence="8">
        <location>
            <begin position="64"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ81069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="304" mass="34375" checksum="90BE2ED67F997D96" modified="2010-06-15" version="1">MLHLYPLLAKPLYRVLLTKPLHHRQFSSTTPNHLIPAILSTTHDLLTTLHTSTHLPYHTLIPLSAIFLRLTITTPLTIYSRRRALRTQRLQPILSAYQHPIARGAKSATTTPQAWELKTRKEMREKRRELWKRWGCQAWKMLLAPVVQIPVWVAASMTLRGMTGIEGLPTWLRGSGEIGGEAWVEEALATEGALWFGDLLIADPYLALPMGFSFLLFANIELQSKLHPPSTRKQRVFTNVLRILSLAAFPLTANAPAALTLYWSTSAMFSLVQNIILAKVMPRGDELEPCKFQDPYSVGDKVRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>D5G980</accession>
    <name>D5G980_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00003188001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430056">
        <property type="protein sequence ID" value="CAZ81073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836882.1">
        <property type="nucleotide sequence ID" value="XM_002836836.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G980"/>
    <dbReference type="GeneID" id="9184359"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003188001"/>
    <dbReference type="eggNOG" id="ENOG502SAPN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_903702_0_0_1"/>
    <dbReference type="InParanoid" id="D5G980"/>
    <dbReference type="OMA" id="PKANITH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF92">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="50"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="96"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="132"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="182"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="212"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="255"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="8">
        <location>
            <begin position="44"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="282"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ81073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="336" mass="37593" checksum="3D9AE43FD91781A8" modified="2010-06-15" version="1">MSQQPPRVDVPVSTEGLLALTWGLNSLVFVVLVIRLVLHRRQKLKGPAIVISDALICVSFFFGITAIATDAWKYSKEIEARTRQLSLHDKETSPKLSFFCGYFVFSAMWFAKGAFVVYYYHLFTNLSKKLRYYLIFVTAFTASTYIFTMLLQTFWCRPIWTNWTLDPLRGCSATGNTTTLPLWSFMNIVTDILILAVPLSVLHTLQLGRRQVVALIVVFLIGGLSIFAAVARFIIIFPKIKKNSKAPIVHTYELWALIEIMSSQLAVCLPALRGWLRGRKDNRSRKAWGGSERKADNAVSGGSGGKKLRDSQDSCPDSGISRVEGGEGLLGNDDRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5G985</accession>
    <name>D5G985_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00003194001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the paxM FAD-dependent monooxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430056">
        <property type="protein sequence ID" value="CAZ81078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836887.1">
        <property type="nucleotide sequence ID" value="XM_002836841.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G985"/>
    <dbReference type="STRING" id="656061.D5G985"/>
    <dbReference type="GeneID" id="9184364"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003194001"/>
    <dbReference type="eggNOG" id="KOG2614">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009665_19_3_1"/>
    <dbReference type="InParanoid" id="D5G985"/>
    <dbReference type="OMA" id="YPIPWID"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000115">
        <property type="entry name" value="Salicylate hydroxylase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050493">
        <property type="entry name" value="FAD-dep_Monooxygenase_BioMet"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13789">
        <property type="entry name" value="MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13789:SF147">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_2G01950)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding" evidence="6">
        <location>
            <begin position="6"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ81078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="438" mass="48377" checksum="1B0DB2FC3B2F28B8" modified="2010-06-15" version="1">MPKPRLNVLIVGAGLGGLSAAIAIRLAGHEVLVLEQASTLSEVGAGIQIPPNSARFLISWGLREAMDRVSVLPETIRLRSYRDGSILNEQPLVPLTEEVYGGPYWHCHRADFHKVLVEKCYAIGGLTIEVRINSMVTEIDFEAPSVTLSTGRTIGADLIVGSDGLKSKCREQFLGRSDPPHLTGDLAYRLLVKAEEMRKHKDLEEFVSKPAINTWMGPQAHAVCYLLKGGGYYNMVLICPDNMPGGVNVMPADPAEVLQLFKSWDPRLKKMISLVSSVSKWRLQNSTEMASWTHPSGKFTLLGDACHATLPYLAQGAAIAVEDGGVLGGLLGKLEDRSQLPGLLRVYEGLRKNRTTKIVVGSTNMRDILHMEDGEEQRRRDAVLLADDIKPGFPNKWRDPVFRNFLFSYDGAREVEIAWERFRERSGSGAVGTFRAML</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9D4</accession>
    <name>D5G9D4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00003257001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430059">
        <property type="protein sequence ID" value="CAZ81127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836936.1">
        <property type="nucleotide sequence ID" value="XM_002836890.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9D4"/>
    <dbReference type="STRING" id="656061.D5G9D4"/>
    <dbReference type="GeneID" id="9182878"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003257001"/>
    <dbReference type="HOGENOM" id="CLU_1448714_0_0_1"/>
    <dbReference type="InParanoid" id="D5G9D4"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ81127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="187" mass="21246" checksum="BBAA4B672A3C386E" modified="2010-06-15" version="1">MKRQKKRMLKEHVLAPPKVPASNPYSTFMSLEGVTVGADAAKKWKALTPEQQQEYAEKGRNLHALGLQNREKWVKTYHPLEVYKANKARKHLKRLGGGKKFDVLKDPRMPKRPTPIGAAFLRDQWNAGTFINPDGSKMPAVLALKHSRDLYEKLSAAEKKVYEDQFAAERAVYKKAMAELFGDISKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9J2</accession>
    <name>D5G9J2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="13">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00003349001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tom22 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430062">
        <property type="protein sequence ID" value="CAZ81185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836994.1">
        <property type="nucleotide sequence ID" value="XM_002836948.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9J2"/>
    <dbReference type="FunCoup" id="D5G9J2">
        <property type="interactions" value="233"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G9J2"/>
    <dbReference type="GeneID" id="9184761"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003349001"/>
    <dbReference type="eggNOG" id="KOG4111">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094333_1_0_1"/>
    <dbReference type="InParanoid" id="D5G9J2"/>
    <dbReference type="OMA" id="QGAQHML"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005742">
        <property type="term" value="C:mitochondrial outer membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045040">
        <property type="term" value="P:protein insertion into mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd22884">
        <property type="entry name" value="TOM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005683">
        <property type="entry name" value="Tom22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12504">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12504:SF0">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM22 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04281">
        <property type="entry name" value="Tom22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ81185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="142" mass="16006" checksum="1502200251E762CE" modified="2010-06-15" version="1">MVELREVEDEHFVHSQPAHSDDEDYTDTDSEISEEDEDDDVDLEESFYERIAALKDMIPLKQRTYISNGFSRTYNFFSKGFALGGKTLWILSTSALVLGVPFSVAITDEQQVAEMEKEMKMTQIATDALTPGAESVLTTPQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9W9</accession>
    <name>D5G9W9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00005109001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430064">
        <property type="protein sequence ID" value="CAZ81312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837121.1">
        <property type="nucleotide sequence ID" value="XM_002837075.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9W9"/>
    <dbReference type="GeneID" id="9188555"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005109001"/>
    <dbReference type="HOGENOM" id="CLU_2814280_0_0_1"/>
    <dbReference type="InParanoid" id="D5G9W9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ81312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="67" mass="7650" checksum="B3F62A9E403C5220" modified="2010-06-15" version="1">MRRVIKCGIILYVSKVRGGKAKKTQRRLVKKAETIARPKTATPIPSHPIPIPIPIPLHTLERKRFFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9Z0</accession>
    <name>D5G9Z0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">receptor protein-tyrosine kinase</fullName>
            <ecNumber evidence="1">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00003463001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.10.1" evidence="1"/>
    <dbReference type="EMBL" id="FN430068">
        <property type="protein sequence ID" value="CAZ81333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837142.1">
        <property type="nucleotide sequence ID" value="XM_002837096.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9Z0"/>
    <dbReference type="GeneID" id="9187056"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003463001"/>
    <dbReference type="HOGENOM" id="CLU_1603954_0_0_1"/>
    <dbReference type="InParanoid" id="D5G9Z0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044912">
        <property type="entry name" value="Egfr_JX_dom"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072661" description="receptor protein-tyrosine kinase" evidence="7">
        <location>
            <begin position="25"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="30"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="49"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ81333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="166" mass="18094" checksum="57728347D237B316" modified="2010-06-15" version="1">MSKLNISPQSLAYLLFILPTFTSALITRQPDDVDEDKQGQVVVSTDGSAPPPPPPPPPMGPKTIVPGLDDGSTIACIVGGISLIVVIPMFVYIWRRRRRLRRARAPPRSLDKVALVDRRGGSIDEQESQLPSPLEKLERTGTSVSFASEVTVMDQVAKPQPVFHAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9Z5</accession>
    <name>D5G9Z5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00003468001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430068">
        <property type="protein sequence ID" value="CAZ81338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9Z5"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003468001"/>
    <dbReference type="eggNOG" id="ENOG502T2BZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1836579_0_0_1"/>
    <dbReference type="InParanoid" id="D5G9Z5"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024316">
        <property type="entry name" value="APQ12"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12716">
        <property type="entry name" value="Apq12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="140" mass="15061" checksum="12C65A0B7E8380A2" modified="2010-06-15" version="1">MDEILFHVMNIAHSATSYTTMLKTHMISPLKQYAASQPDLMNIALLLVIVYLSLSILGMASRWIYSVVITTLRLLLFVVVIGGGIWVWQRGPQKSQSDFLKVVEVVSEGVSAAAAGKGPGGGPGGMGGAGAGFERQRYEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>D5GA90</accession>
    <name>D5GA90_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">intramembrane prenyl-peptidase Rce1</fullName>
            <ecNumber evidence="10">3.4.26.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00005185001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Hydrolyzes the peptide bond -P2-(S-farnesyl or geranylgeranyl)C-P1'-P2'-P3'-COOH where P1' and P2' are amino acids with aliphatic sidechains and P3' is any C-terminal residue.</text>
            <dbReference type="EC" id="3.4.26.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase U48 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.26.1" evidence="10"/>
    <dbReference type="EMBL" id="FN430075">
        <property type="protein sequence ID" value="CAZ81444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837253.1">
        <property type="nucleotide sequence ID" value="XM_002837207.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GA90"/>
    <dbReference type="FunCoup" id="D5GA90">
        <property type="interactions" value="281"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GA90"/>
    <dbReference type="MEROPS" id="G05.002"/>
    <dbReference type="GeneID" id="9185726"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005185001"/>
    <dbReference type="eggNOG" id="KOG4130">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049909_1_0_1"/>
    <dbReference type="InParanoid" id="D5GA90"/>
    <dbReference type="OMA" id="HSFCNWC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071586">
        <property type="term" value="P:CAAX-box protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039731">
        <property type="entry name" value="Rce1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003675">
        <property type="entry name" value="Rce1/LyrA-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13046:SF0">
        <property type="entry name" value="CAAX PRENYL PROTEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13046">
        <property type="entry name" value="PROTEASE U48 CAAX PRENYL PROTEASE RCE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02517">
        <property type="entry name" value="Rce1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="56"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="219"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="263"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="CAAX prenyl protease 2/Lysostaphin resistance protein A-like" evidence="12">
        <location>
            <begin position="139"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ81444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="293" mass="33443" checksum="7B0831A8E9170481" modified="2010-06-15" version="1">MSSTPEQQHLEFTPFLANLFSVIYTSSYVGILYLSHLTRPQPGLARDTPSVIRARIRAVTVVTAVALANSAPITYFYSPHKTYLDIATFLGLYVKLPLTLDILRCFLLTGTLFLGPLVRRLWFDHGWRELQGDVDKMFTTWIGWRNYVAGPFTEEIVFRACVVPLHLLAGRSPGTIVFITPLFFGIAHIHHAYEFYINNPNRIFVMIVRSLFQFTYTTLFGWFATFVFLRTGSVWASIIVHSFCNFMGLPDFGPVDGPRWRSAVYYALLVAGAFSFYKLLWLLTGSQHALVRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>D5GB33</accession>
    <name>D5GB33_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00005424001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430086">
        <property type="protein sequence ID" value="CAZ81726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837535.1">
        <property type="nucleotide sequence ID" value="XM_002837489.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GB33"/>
    <dbReference type="STRING" id="656061.D5GB33"/>
    <dbReference type="GeneID" id="9187967"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005424001"/>
    <dbReference type="eggNOG" id="ENOG502RXGX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052530_0_1_1"/>
    <dbReference type="InParanoid" id="D5GB33"/>
    <dbReference type="OMA" id="YAGINKG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015814">
        <property type="entry name" value="Pgluconate_DH_NAD-bd_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09130">
        <property type="entry name" value="DUF1932"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphogluconate dehydrogenase NAD-binding putative C-terminal" evidence="1">
        <location>
            <begin position="220"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="356" mass="38198" checksum="85FABD1BA6B72312" modified="2010-06-15" version="1">MAGATVAILSPGEMGSGIACLLRAHQFRVITSISGRRDSLSSSSYTYDRAVASKIEFVSSDVDLINQADFILSIVPPSEALPTARRVGYAYTYAARGNKLPLYYFDLNAVSPNLAEKVEDVFSKPTAGANGAVRFIDGAILGGAPEFMQGPGGVGGVWKKPRVVVSGKEKLVGDSGHLLEEVLGLTWVGDRVGQASGLKMCFASLTKGLTGLAVQSFTTASNLSLLPALKEELAIFCSPLLTFLESAVPAVPPRAYRFVREMEEIADTHAEAGFSRAVFSGIADIYRGVAQDEVLGKERSGERNRGQTVDDFADTYGEGMRRREEVNRNGNNGGVQDGMELEKKWMGRAVERMAVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>D5GB49</accession>
    <name>D5GB49_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Endonuclease III homolog</fullName>
            <ecNumber evidence="12">3.2.2.-</ecNumber>
            <ecNumber evidence="12">4.2.99.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Bifunctional DNA N-glycosylase/DNA-(apurinic or apyrimidinic site) lyase</fullName>
            <shortName evidence="12">DNA glycosylase/AP lyase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">NTH1</name>
        <name evidence="15" type="ORF">GSTUM_00005447001</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Bifunctional DNA N-glycosylase with associated apurinic/apyrimidinic (AP) lyase function that catalyzes the first step in base excision repair (BER), the primary repair pathway for the repair of oxidative DNA damage. The DNA N-glycosylase activity releases the damaged DNA base from DNA by cleaving the N-glycosidic bond, leaving an AP site. The AP lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination. Primarily recognizes and repairs oxidative base damage of pyrimidines.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66592"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:16897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17067"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:157695"/>
            <dbReference type="ChEBI" id="CHEBI:167181"/>
            <dbReference type="EC" id="4.2.99.18"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 12">Belongs to the Nth/MutY family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.-" evidence="12"/>
    <dbReference type="EC" id="4.2.99.18" evidence="12"/>
    <dbReference type="EMBL" id="FN430086">
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        <property type="interactions" value="482"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
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        <property type="entry name" value="HhH_motif"/>
    </dbReference>
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        <property type="entry name" value="NTH1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43286">
        <property type="entry name" value="ENDONUCLEASE III-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43286:SF1">
        <property type="entry name" value="ENDONUCLEASE III-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00633">
        <property type="entry name" value="HHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01155">
        <property type="entry name" value="ENDONUCLEASE_III_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4 12" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8 12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10 12" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HhH-GPD" evidence="14">
        <location>
            <begin position="207"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="130"/>
            <end position="151"/>
        </location>
    </feature>
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            <begin position="1"/>
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        </location>
    </feature>
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            <dbReference type="ARBA" id="ARBA00008343"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00044632"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAZ81742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="459" mass="51075" checksum="172C6EB27111B856" modified="2010-06-15" version="1">MARAGRAAARAGAASSLSAVVATTVTRVATTRPTTMQTRAGVPMGDDYSSSGLSSPPSDLGNYSDEEREVKKEEDEEEGKNGSIVSRFFQSPSPGKGARKAPRKAIALVKKEPTPELGDIAPVLIKEEKEEKKEKGIGQRKPRGKKVKDEEHVKVEAPENWEEVYEKLREMRKKVKAPVDTMGCERLGDKAATPKLRRFHTLISLMLSSQTKDTINAVAMKGLREQLPGGLCLESILEVEPKRLDELIRIVGFHNRKTEYIKKAAVIIRDKHGGDIPDTFEGLTALPGVGPKMAHLCLSAAWDRTEGIGVDVHVHRICNLWDWVKTTTPEGTREALQAWLPRDKWREINFLLVGFGQTICLPRGRKCGECALSSGLCGAAYMEPKKVKAKKGKRVIGEDSDEGFDDDNYAAPERRVRPKRRRVRVEVKEEEEMEVEDEDALVADIEDAVPNLAKFRYKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>D5GB80</accession>
    <name>D5GB80_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00003781001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430087">
        <property type="protein sequence ID" value="CAZ81773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002837536.1"/>
    </dbReference>
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    <dbReference type="KEGG" id="tml:GSTUM_00003781001"/>
    <dbReference type="HOGENOM" id="CLU_1338396_0_0_1"/>
    <dbReference type="InParanoid" id="D5GB80"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="149"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="205" mass="23081" checksum="07B8D4E58078F28C" modified="2010-06-15" version="1">MNSTVHSEWQKLRIHAAPQDCRNRFGHVPRDLSRPHTPPSLSPSPAPSQCSYYTDHCDDADDFEIEEIEDEYGDSGVELLIGEYEEPSPDYGREMYDSDSDDESQREIVYSFGMLSAGRGPSPIAKAMAQQHFKFGVQPRKAKRSYTESVGSSDSDYDSGFDSQSSERRIRRRIIRQRDGSVEAVSVGTSYAGSQTSDYEVDMEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBF1</accession>
    <name>D5GBF1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00000441001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430093">
        <property type="protein sequence ID" value="CAZ81844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBF1"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000441001"/>
    <dbReference type="HOGENOM" id="CLU_3423350_0_0_1"/>
    <dbReference type="InParanoid" id="D5GBF1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="23" mass="2767" checksum="C373EA59E9FE78FF" modified="2010-06-15" version="1">MQQDKRVWQQNRDLAGLQNHSIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBN6</accession>
    <name>D5GBN6_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein bS18m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00005494001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bS18 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ81886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837695.1">
        <property type="nucleotide sequence ID" value="XM_002837649.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBN6"/>
    <dbReference type="STRING" id="656061.D5GBN6"/>
    <dbReference type="GeneID" id="9182923"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005494001"/>
    <dbReference type="eggNOG" id="KOG3162">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082177_0_0_1"/>
    <dbReference type="InParanoid" id="D5GBN6"/>
    <dbReference type="OMA" id="MPATHKH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.640.10:FF:000013">
        <property type="entry name" value="37S ribosomal protein S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.640.10">
        <property type="entry name" value="Ribosomal protein S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001648">
        <property type="entry name" value="Ribosomal_bS18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036870">
        <property type="entry name" value="Ribosomal_bS18_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13479">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13479:SF40">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN BS18M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01084">
        <property type="entry name" value="Ribosomal_S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46911">
        <property type="entry name" value="Ribosomal protein S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="39"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ81886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="202" mass="22754" checksum="737B68EC65DFA792" modified="2010-06-15" version="1">MSSRLTLSFLPRLSLLRPPPLARTAKRCFTAAPHLQNDIPPPSNPPSTTPAPKDLTQKLTASLSNLAPASLPEDYISDQDLQKLQSRFLPGDIYAPHDLSHAQQRSRRTRRSPRFDVCDLVGINPVKEYKNFCMLSEYVTEMGRIKHSSETGLRPVNQRRMAKAIRRAIALGFMPATHKHPEYLMHMRESREGRGGGQRGGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBU2</accession>
    <name>D5GBU2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00005580001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ81942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837751.1">
        <property type="nucleotide sequence ID" value="XM_002837705.1"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GBU2"/>
    <dbReference type="GeneID" id="9182979"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005580001"/>
    <dbReference type="eggNOG" id="KOG0949">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002305_2_0_1"/>
    <dbReference type="InParanoid" id="D5GBU2"/>
    <dbReference type="OMA" id="GYFHRLC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18025">
        <property type="entry name" value="DEXHc_DDX60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001039">
        <property type="entry name" value="ATP-dependent RNA helicase DDX60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055124">
        <property type="entry name" value="PIN-like_DDX60"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052431">
        <property type="entry name" value="SKI2_subfamily_helicases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059032">
        <property type="entry name" value="WHD_DDX60"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44533">
        <property type="entry name" value="DEAD/H RNA HELICASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44533:SF4">
        <property type="entry name" value="DEAD_H RNA HELICASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23002">
        <property type="entry name" value="PIN-like_DDX60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26076">
        <property type="entry name" value="WHD_DDX60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="6">
        <location>
            <begin position="811"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="1255"/>
            <end position="1428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="169"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="565"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1132"/>
            <end position="1156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1732"/>
            <end position="1763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="580"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1732"/>
            <end position="1743"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1746"/>
            <end position="1758"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ81942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1788" mass="200092" checksum="F2C3173908B4140C" modified="2010-06-15" version="1">MDPAMQLRTIREWYAKLRSIRVDLVGDYAGEEFFIIEGDSLLRWVFADERIDFDTGFQLLHAVYVVESFLQQLSSRRCRFSVVFLDISRNACIPFSPTAEQNAWKYLFARSAIIEHMKHIDHPQGLVSSFESLADPEFRRYLDSTRPYFVMYHDGADAIHFKKAHKKPDWEAGGASERGNGNDTEGNDDADSPQTGYVDISLDGGFNQEGLLKAGIREWMSLGFSIALMNEIVFKDSKIFTYVLETNLYTLEPVDIRVSPAETPPPGPEYSQAPDWKSLRSARERLAVAAVAHALRLDEGKHEDLASIFLVHIILLSQLTLPARFLPPFNVDEAVSAAISPARDFLRRLIILSGDLLRIGAVSLEDAQNDLCDYVDGRLLLKLILVPEELSEVVKQRFSDLADHVETLCGESLELPILSKSVNISFTEPPAVWGCKILAFSNPAFDKYLEQIRLPGDILAEEGSEDHEEEDEQAYYEKTHWHNPRNPLVTARIRAQRALPKPVAKVRGAVAVAPGAKTQDQDRAVRRAAGRARKWEQVYLNQMYQYASSLTDSIDGSLNPKLVTVGEKAKKPQAAGKKTMVKEEKNAKPNKKDGPSKGKGSGKLSAADIISQNNATKKAGREAKLQDSWKSICDEVASIKDDETAILRLETWLAASQKAIAAAVAENKEWPFIEAEARLYKIQLLQRIWVGYCRRREQDHGYAAASALFNEARLVLFSGGMTRKVHQILNNIFEAFGIPKPPSDPLASLPDRKILFTKVWDGKCEGFDVKLGMTSEEFQLLHCGPWMDRNMDSQSDPRVPFKPDGWQRKVLDELDQDNSLFVVAPTSAGKTFIAFYAMEKVLKESDEGVLVYVAPTKALVNQIAAEVISRYKKNYRYAGKTVWAIHTRDYRMNNANECQILITVPDILQIMLMSPSNAKSWTPRVKRIIFDEVHSIGNAEDGLVWEQLLLLAPCPIIALSATVGNPEEFGSWLASTQKSVGVKLTVVQHPYRYSDLRKFVYSPKKGMTSFEGMPQKVSRFNQLEMADYMRVVHPVAALANTKQGIPNDMSLEPKDCLQLYWAMSKCQTPECPLPKDLDFKEVFGTEGKVVTKAEVVKWEEDLKKVLLNWMEDAEPLFSEIAKLLAAEAAKTDAPSKGYGDGGRSSGAPAEGDGPLVVDLPPPSCTREYLTKTTLPLLQTLHSANALPAILFSYDRLMCEKICIMICSQLETAEKNWRENSPKWQKIIKDWEKYKADRSSRGSKKAKVVLPPGSTKADQMRENAEYDSSFWSSFDPNHPSPEFSFADFKKHAKSDLQIDIDNLIRWGIPEVLVHAFSRGIGVHHSGMNRKYRQAVEMLFRKGYLRVVIATGTLSLGINMPTRSSVFVGESVYLNSLNYRQAAGRAGRRGFDNLGNVVFHAFSLAKVERIMSSKLPSLIGHFPISTSLVLRLFILLHNSNESDHAKHTINTLLTQGKLVIGGGEFKEQVLHHLRFSIEFLRRQKLLGSNGRPLNFAGLTSHLYYVENSSFALHVLLCNGYLKELCKNINKSPDSICLELMLVLANIFGREQKYFSRHKSLPPLPAAAAELLTKQNERTLRTYVTYVESFVKSYCKDLDNVLPFSGLECGGKGTADESNVKSRSSFVALSGHGDNFTSVDDLTSSVRRGVLIDGASVPHLRADDSSTVNGYLYYFYIHGDVKKLDQEHGIPAGSVWFVLKDFSLILAAIEAGLTCFIQDGPGAYYDFKDEGDDVDGDIHEDDEDDRESVTTTDDGSSGVSSEATDPGFKGVLKAVKILRGRFEEKFKKMWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBZ9</accession>
    <name>D5GBZ9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00005729001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430098">
        <property type="protein sequence ID" value="CAZ82042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837851.1">
        <property type="nucleotide sequence ID" value="XM_002837805.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBZ9"/>
    <dbReference type="GeneID" id="9181311"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005729001"/>
    <dbReference type="HOGENOM" id="CLU_1876958_0_0_1"/>
    <dbReference type="InParanoid" id="D5GBZ9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="136" mass="14984" checksum="17657EA5CC2AA34E" modified="2010-06-15" version="1">MLAPFSMIPFCAPAYCTSTVHLILGPHLPVLPLILCILSFLHFCIHTCLFVIYHTGTPSVAFDTSTSPPPLNKAVNIIREILSTPIAGPTAPANRLPFRLPSAYLPVLFSSLTPPSSSSPHLYSIYRRFRRRPLSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GC72</accession>
    <name>D5GC72_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 12">Adenylosuccinate lyase</fullName>
            <shortName evidence="12">ASL</shortName>
            <ecNumber evidence="6 12">4.3.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 12">Adenylosuccinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00000611001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>(2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + fumarate</text>
            <dbReference type="Rhea" id="RHEA:23920"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:58443"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="EC" id="4.3.2.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>N(6)-(1,2-dicarboxyethyl)-AMP = fumarate + AMP</text>
            <dbReference type="Rhea" id="RHEA:16853"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="4.3.2.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 12">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer. Residues from neighboring subunits contribute catalytic and substrate-binding residues to each active site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.2" evidence="6 12"/>
    <dbReference type="EMBL" id="FN430103">
        <property type="protein sequence ID" value="CAZ82115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837924.1">
        <property type="nucleotide sequence ID" value="XM_002837878.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GC72"/>
    <dbReference type="FunCoup" id="D5GC72">
        <property type="interactions" value="682"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GC72"/>
    <dbReference type="GeneID" id="9184791"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000611001"/>
    <dbReference type="eggNOG" id="KOG2700">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030949_1_1_1"/>
    <dbReference type="InParanoid" id="D5GC72"/>
    <dbReference type="OMA" id="VQENAMK"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00132"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00075">
        <property type="reaction ID" value="UER00336"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070626">
        <property type="term" value="F:(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido) succinate lyase (fumarate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004018">
        <property type="term" value="F:N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03302">
        <property type="entry name" value="Adenylsuccinate_lyase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.275.60:FF:000001">
        <property type="entry name" value="Adenylosuccinate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.40.30:FF:000005">
        <property type="entry name" value="Adenylosuccinate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.40.30">
        <property type="entry name" value="Fumarase/aspartase (C-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.200.10">
        <property type="entry name" value="Fumarase/aspartase (Central domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019468">
        <property type="entry name" value="AdenyloSucc_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004769">
        <property type="entry name" value="Pur_lyase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00928">
        <property type="entry name" value="purB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43172">
        <property type="entry name" value="ADENYLOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43172:SF1">
        <property type="entry name" value="ADENYLOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10397">
        <property type="entry name" value="ADSL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00998">
        <property type="entry name" value="ADSL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="L-aspartase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="8 12" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Adenylosuccinate lyase C-terminal" evidence="13">
        <location>
            <begin position="373"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00017058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ82115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="481" mass="54531" checksum="780EFD9873EE7ED3" modified="2010-06-15" version="1">MSNNDIYQTPLASRYASEEMAQLFSNKTRFRTWRQLWVWLAKAEKQLGLPISDEAIAQMEAHLDLTDEDFKVAAEEEKRRRHDVMAHVHTFGQVAPAAAGIIHWGATSCYCTDNADLLFLRQGLDIVLPKLANVIYKLSNFALEHKDLPALGYTHYQPAQLTTVGKRACLWIQDLLWDLRNLTRARSDLGFRGVKGTTGTQASFLAIFNGDHDRVEELDRLVTQLAGFEYAYPITSQTYSRKVDADVIYAMSSFGATCQRIGGDIRHLAATKEMEEPFEKDQIGSSAMAYKRNPMRSERMCSLGRHLANLCKDALDTYSQQWFERTLDDSAIRRIDIPHAFLTTDVLLRILDNVVSGLVVYPAVIARRIREELPFMATENFIMRMVALGASRQDTHEAIRVLSHQASAIVKQEGKDNDLIERIKKDKFFEPIWGEIDGLMDPRTFVGRAPEQTVKFIEKEVKVALEPWTGALSGEVSELNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCE2</accession>
    <name>D5GCE2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Actin-related protein 2/3 complex subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00005849001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the Arp2/3 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasm</location>
            <location evidence="1 6">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ARPC3 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430109">
        <property type="protein sequence ID" value="CAZ82185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837994.1">
        <property type="nucleotide sequence ID" value="XM_002837948.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCE2"/>
    <dbReference type="FunCoup" id="D5GCE2">
        <property type="interactions" value="933"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GCE2"/>
    <dbReference type="GeneID" id="9186367"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005849001"/>
    <dbReference type="eggNOG" id="KOG3155">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094365_1_0_1"/>
    <dbReference type="InParanoid" id="D5GCE2"/>
    <dbReference type="OMA" id="TPSKWWL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044396">
        <property type="term" value="P:actin cortical patch organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051654">
        <property type="term" value="P:establishment of mitochondrion localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007163">
        <property type="term" value="P:establishment or maintenance of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1760.10:FF:000002">
        <property type="entry name" value="Actin-related protein 2/3 complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1760.10">
        <property type="entry name" value="Actin-related protein 2/3 complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007204">
        <property type="entry name" value="ARPC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036753">
        <property type="entry name" value="ARPC3_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12391">
        <property type="entry name" value="ARP2/3 COMPLEX 21 KD SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04062">
        <property type="entry name" value="P21-Arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016315">
        <property type="entry name" value="ARP2/3_P21-Arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69060">
        <property type="entry name" value="Arp2/3 complex 21 kDa subunit ARPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR016315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ82185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="190" mass="21646" checksum="53681DF92F5D04EB" modified="2010-06-15" version="1">MPAYHSAFLQDTDVRVIGNFTLLPLRTRTRGPAYTLSPLPANITNDLDIDPDGEHYDILDETITLFRANTLFRNFEIKGPADRVLIYGILFVSDCLSRIKPSMSLRDSEKALQTAALDHFALPGDPAFPLNALYQPPRDRMEAEALRGYISQMRQELVIRLLNRVYADGSGKPSKWWLSFTKRRFMGKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCG8</accession>
    <name>D5GCG8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00005884001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430109">
        <property type="protein sequence ID" value="CAZ82211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838020.1">
        <property type="nucleotide sequence ID" value="XM_002837974.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCG8"/>
    <dbReference type="STRING" id="656061.D5GCG8"/>
    <dbReference type="GeneID" id="9186393"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005884001"/>
    <dbReference type="eggNOG" id="KOG4696">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013053_3_0_1"/>
    <dbReference type="InParanoid" id="D5GCG8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041298">
        <property type="entry name" value="UBZ3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012462">
        <property type="entry name" value="UFSP1/2_DUB_cat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07910">
        <property type="entry name" value="Peptidase_C78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51907">
        <property type="entry name" value="ZF_UBZ3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="UBZ3-type" evidence="9">
        <location>
            <begin position="66"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="102"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="111"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="145"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="172"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="206"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ82211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="594" mass="67556" checksum="E5C720F1740055F6" modified="2010-06-15" version="1">MAGAVEVYECPWCRFKSGAEYTILMHIEAIHTPDSPFMIKDEHGNRDISSEDLVDTDFDIPPEDDAETTYILCTEDGCEENVLLMDLQTHLDFHAAEQISMEDVRSQTPVSGDDSGSYSGSQDMVLSEQGKSRYGYGRSEGSIEGGRRERRENKTLIASRERDKNGYKLSFAQDQGSSSLVPSKTPSHLKSPKRSHKLVFGLPVKDPWRQREKEKEKERERERELERERDRERAREVRRPVERSVVRPIDGQNLPGQQQTVSSNHILTPVKRLGKSDLGPYAHEAQMPAWLRKELEQGGKLTKETRLDHAAGRLVRVVTVSNETPDLIPVIALLCEHDPRVVRAWVCHPGTKHVGKQIRGEGGFCGYRNIQMVVSYIQNAYPRGSHPFEGRIPTILRLQDWIEEGWDKGINPSARLETGGIKGTRKYIGTSEAQTMFVNLNIPCRPHSFTRSEGLKTQEKLLDWVENYFSDGGILPEAAQVKVYQTQKPPIYFQHSGHSMTIVGCETNRDGSKNLLVFDPFFTPSAPMKNLIGARSLSHRMDPELLLRAYRRRMNYLQKYKEFEVITFVPPIASHYNKSIAEGGSGGRIHYYFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCJ1</accession>
    <name>D5GCJ1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00005914001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430109">
        <property type="protein sequence ID" value="CAZ82234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838043.1">
        <property type="nucleotide sequence ID" value="XM_002837997.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCJ1"/>
    <dbReference type="GeneID" id="9186416"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005914001"/>
    <dbReference type="HOGENOM" id="CLU_1788223_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCJ1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="145" mass="16075" checksum="94045342C33DC950" modified="2010-06-15" version="1">MPPHFVTFLLASSTTQRKYSTGLGLRNSDRKTRAICLNSQPRNPTTPGLHLPRRAPSPSSRTGLSPPRLLKSTMASSSNRHSPICPRCLGARPTTTNRTVLASPASLRKKLLRRRADRTAVASRPIMQDKRASPGRIRNVWRMPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCL1</accession>
    <name>D5GCL1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">4-hydroxy-3-methoxy-5-polyprenylbenzoate decarboxylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">COQ4</name>
        <name evidence="8" type="ORF">GSTUM_00000708001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Lyase that catalyzes the C1-decarboxylation of 4-hydroxy-3-methoxy-5-(all-trans-polyprenyl)benzoic acid into 2-methoxy-6-(all-trans-polyprenyl)phenol during ubiquinone biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 4-hydroxy-3-methoxy-5-(all-trans-polyprenyl)benzoate + H(+) = a 2-methoxy-6-(all-trans-polyprenyl)phenol + CO2</text>
            <dbReference type="Rhea" id="RHEA:81179"/>
            <dbReference type="Rhea" id="RHEA-COMP:9551"/>
            <dbReference type="Rhea" id="RHEA-COMP:10931"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:62731"/>
            <dbReference type="ChEBI" id="CHEBI:84443"/>
            <dbReference type="EC" id="4.1.1.130"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="7">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of a multi-subunit COQ enzyme complex, composed of at least COQ3, COQ4, COQ5, COQ6, COQ7 and COQ9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion inner membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
            <orientation evidence="7">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the COQ4 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430114">
        <property type="protein sequence ID" value="CAZ82254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838063.1">
        <property type="nucleotide sequence ID" value="XM_002838017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCL1"/>
    <dbReference type="FunCoup" id="D5GCL1">
        <property type="interactions" value="501"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GCL1"/>
    <dbReference type="GeneID" id="9186437"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000708001"/>
    <dbReference type="eggNOG" id="KOG3244">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061241_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCL1"/>
    <dbReference type="OMA" id="YYERHFH"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031314">
        <property type="term" value="C:extrinsic component of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120539">
        <property type="term" value="F:4-hydroxy-3-methoxy-5-polyprenylbenzoate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03111">
        <property type="entry name" value="Coq4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007715">
        <property type="entry name" value="Coq4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027540">
        <property type="entry name" value="Coq4_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12922">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12922:SF7">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS PROTEIN COQ4 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05019">
        <property type="entry name" value="Coq4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2 7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="7">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00081568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ82254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="262" mass="29860" checksum="4934A94F023B924F" modified="2010-06-15" version="1">MNSLVLRPVIRRTGLARDLPKVAFTSQFVPFTSLSRPPPNYEGHVPLTIPERGFLAVGSAVMSLLSPRRADLIAALGEATATPYFIYRLRDAMLLSPTGRRILKDRPRINSTTMSMGKLRELPDGTVGKEYVAWLDKEGVSPDTRSQVRYIDDPECAYVMQRYRECHDFYHALTGLPIMVEGEVALKAFEFANTLIPMTGLSMFAVARLKPKERRRILTTYFPWAIRNGLQAKEVMNIYWEEELETDVKELRKRIGLEKPPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5GD23</accession>
    <name>D5GD23_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00000944001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430136">
        <property type="protein sequence ID" value="CAZ82416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838225.1">
        <property type="nucleotide sequence ID" value="XM_002838179.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GD23"/>
    <dbReference type="FunCoup" id="D5GD23">
        <property type="interactions" value="1081"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GD23"/>
    <dbReference type="GeneID" id="9183878"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000944001"/>
    <dbReference type="eggNOG" id="KOG1539">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002774_3_0_1"/>
    <dbReference type="InParanoid" id="D5GD23"/>
    <dbReference type="OMA" id="PMEIACE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034388">
        <property type="term" value="C:Pwp2p-containing subcomplex of 90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007319">
        <property type="entry name" value="WDR36/Utp21_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22840">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22840:SF12">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25168">
        <property type="entry name" value="Beta-prop_WDR36-Utp21_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04192">
        <property type="entry name" value="Utp21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="153"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="WDR36/Utp21 C-terminal" evidence="5">
        <location>
            <begin position="284"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ82416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="472" mass="51757" checksum="29A64549CA943F9F" modified="2010-06-15" version="1">MRDDAQSFVFSEKAGGRRSRNPPQSGEKGTWANREFSSSNPVTCIATVIGEGGAGNAENAARDLAGIITGHRDSKSGSLWSFENKSLGGRWAQTTDGGLVRSAGVSSCGTFCFIGSSNGRMAMYNIQSGIKRRQFPEPVSAATAKRLKNMRELWGAQGRISDFCFSKDGRWVLGASADRVIRVWDLPTGHLIDGIRTKSDATAMAFSGTGEFLATAHVDSVGINLWTNRTLFRHVPTRHLDEKDIVDMTAPFASGEGGVGVIEAALNPQGDGEDDCTGTYTTVDQLSSNMLTMSLIPRARWQTLLNLDVIEQRNKPKEAPGTPEKAPFFLPPLGHTAERSRILRMSRDSGESTFTKLLHEAADGNNRDYTVILAHLKALSPSTADLEIRSLQVNELVPFVRATTQRLRSRRDYQLVESWVNVFLKCHPEGVMSEEDVRDELRKWMVEQKREAGRLAQLVGYCSGVAGFLSGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDY6</accession>
    <name>D5GDY6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00001100001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430155">
        <property type="protein sequence ID" value="CAZ82729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838538.1">
        <property type="nucleotide sequence ID" value="XM_002838492.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDY6"/>
    <dbReference type="GeneID" id="9184524"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001100001"/>
    <dbReference type="HOGENOM" id="CLU_3410728_0_0_1"/>
    <dbReference type="InParanoid" id="D5GDY6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ82729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="29" mass="3460" checksum="A2CC456363D1529B" modified="2010-06-15" version="1">MRILKSHPLLRMVNSYIIDSPQPSNISYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>D5GE18</accession>
    <name>D5GE18_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00001182001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430161">
        <property type="protein sequence ID" value="CAZ82761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838570.1">
        <property type="nucleotide sequence ID" value="XM_002838524.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GE18"/>
    <dbReference type="GeneID" id="9183092"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001182001"/>
    <dbReference type="eggNOG" id="ENOG502RZYG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_270035_0_0_1"/>
    <dbReference type="InParanoid" id="D5GE18"/>
    <dbReference type="OMA" id="PRRIMNG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.50.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36182">
        <property type="entry name" value="PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G10930)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36182:SF1">
        <property type="entry name" value="PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G10930)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1141"/>
            <end position="1161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1183"/>
            <end position="1206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="231"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="274"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="538"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="660"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="734"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="945"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="314"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="336"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="379"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="567"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="660"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="674"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="735"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="751"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="809"/>
            <end position="818"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ82761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1208" mass="127898" checksum="4CA245DEC614B7F7" modified="2010-06-15" version="1">MDLKYPAPLNHPNNPNTNAGTADFSYNSPISAAQYPCKGHHKLIGTPAGKSVATWAAGSTQNFTLSGTAVHGGGSCQASISEDGGKTFRVVRSYVGGCPAVGKAFEFVVPKETKSGVALFAWTWFNNLGNREMYMNCAAVTISDGGSKGLSDLPEIFQVNLGSGCETVPGSDVLFPAPGNDAVIVNPAATAPIPPGYLAVLGRGLGARAQKRGIGNSSAGIMAASTPHPQLLASVPSTESSPPPSPVPLDASASIKQSSGVEFKHRFLPLPFHRPLAASTSDHPNIPAAAPDQSARPRLPGRQETPPSPPNSVLLDQSPTSPSQPTLAQPIADHPSSPQSISGTNVEGPGVTEHQTPPPSLKDRLARAFKGHKRGESDVSMSTNINPRDGASTSNHRHNNSGSTLVTSATTTAQNIRDNNNNSTILQTFASKTNLGFSPTKPDFYTHQWASPIRPNSQNHYTYGHGGTKRRIMNGGEVRVEILPRPSVIAERSTHEEGGKPGLFEEYKEQGGGDAYKSHGVDHVRCDQHEDGYLFEAPNPLRHGGRRSQNDLDDYLPPLNLSLGTMGDSSPRSRTATSLETVLSGDSDSSGGSRERRFVEESTASFNFEDLRAGSENDNYFAGFPSPNPDSGSALTGLCGSSGMASSGVLASLSQGDLSTQARSNSSLGGYTTGKSSSGAVGRSSSSHLLALQRGQVPGSGTSPMEDAVGKELKRLSKISAGSGVSGVAIVVTSDEPDSDFGEGSDDDADDQRWTREEKGKGRAPRSVGSGNGSMAERSGSDARLGNYKEHHRSGSGMSRVSGWSLGDQSGGTGSGSEGGDDDSRELLKGVDQTKKRKLKSQVAPKGVLVHEGDGRYDFVYRMRRTPDETPILVPQYTFSSESSFPNRNALTLPIPAKISSPNLRGADHIPLATRYGSSPITKPEGAVVRSPDLNDSYYGAVIRGGQADDSPAHSARYSGSHKSISQSMSDSQMGELIHPAHRQASRGGRDRGVVSMGSLEGIVGIGLRPMPSPGLSGGKFVGSSLADTSDIGHPLEDTTAKSSINQKRVRMSANLDEIAPAPQHYFEREPEVRKKRRSYLPPALGPRPMRPKDLRRSMTPHLYAPRPLGDSWKDLADIESLPDNEQTLEYRKRERRVGRLLLVFCMLFPPLLLVVAWGGFDVTVEGWTSGQLKGVGRVEKRIAAWVGGTFAVCVVAGIIAGAVVVSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>D5GE73</accession>
    <name>D5GE73_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00006407001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430164">
        <property type="protein sequence ID" value="CAZ82816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838625.1">
        <property type="nucleotide sequence ID" value="XM_002838579.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GE73"/>
    <dbReference type="FunCoup" id="D5GE73">
        <property type="interactions" value="402"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GE73"/>
    <dbReference type="GeneID" id="9185472"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006407001"/>
    <dbReference type="eggNOG" id="KOG1530">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089574_0_0_1"/>
    <dbReference type="InParanoid" id="D5GE73"/>
    <dbReference type="OMA" id="REPYELF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004792">
        <property type="term" value="F:thiosulfate-cyanide sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01519">
        <property type="entry name" value="RHOD_HSP67B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44086">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE RDL2, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44086:SF10">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE_RHODANESE-LIKE DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072087" evidence="1">
        <location>
            <begin position="20"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="2">
        <location>
            <begin position="79"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ82816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="178" mass="19754" checksum="D2AF2AAF0CA02CBE" modified="2010-06-15" version="1">MSPAKRLLTTLLLPRLSTGTPISRRKIATIPTHLHAPPFFCTPPRQQPPLSVSKRYSTAQSPNTKLYDYEAIKSLTKTYPRTCALIDVREPAEFAAGAIPMAVNIPIASSPDALTLSPEEFEDRFGFAKPELEKEVVFYCKAGIRSSAAAQVAVGVGYRRVGEYRGSWVDWEKRERGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>D5GE96</accession>
    <name>D5GE96_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00001233001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EMBL" id="FN430168">
        <property type="protein sequence ID" value="CAZ82839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838648.1">
        <property type="nucleotide sequence ID" value="XM_002838602.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GE96"/>
    <dbReference type="STRING" id="656061.D5GE96"/>
    <dbReference type="GeneID" id="9185494"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001233001"/>
    <dbReference type="eggNOG" id="KOG1812">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017097_0_0_1"/>
    <dbReference type="InParanoid" id="D5GE96"/>
    <dbReference type="OMA" id="IQIEVFR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20353">
        <property type="entry name" value="Rcat_RBR_RNF216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051628">
        <property type="entry name" value="LUBAC_E3_Ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047546">
        <property type="entry name" value="Rcat_RBR_RNF216"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047544">
        <property type="entry name" value="RING-HC_RBR_RNF216"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058758">
        <property type="entry name" value="UBA_RNF216"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22770:SF42">
        <property type="entry name" value="FINGER PROTEIN (ZIN), PUTATIVE (AFU_ORTHOLOGUE AFUA_4G03910)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22770">
        <property type="entry name" value="UBIQUITIN CONJUGATING ENZYME 7 INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26200">
        <property type="entry name" value="Rcat_RNF216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26191">
        <property type="entry name" value="RING-HC_RBR_RNF216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26112">
        <property type="entry name" value="UBA_RNF216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="321"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="E3 ubiquitin-protein ligase RNF216 UBA" evidence="8">
        <location>
            <begin position="96"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="E3 ubiquitin-protein ligase RNF216 RING finger HC subclass" evidence="9">
        <location>
            <begin position="216"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF26112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF26191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ82839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="571" mass="64497" checksum="5A71B443E820B7E6" modified="2010-06-15" version="1">MLSLLSSPKGKGPRGGRETLSTSLDPEEAADQARVASLNRAIEILNELFPNVDVDEFRHMLSTFSEESRLHVITEELLKKSKDGRVQRRVVEPWEKFRSEAYIVAAKNVLYKEFKGLAHSTIKAVMAENNFDYARSRLTLMDIAGKSWRFSVANFFRGARSGIPPEPTLTATGCKELDDELFSLGKPTRDRQIDEDLKVAKLLNEAEHAVSSEMMEYGHFFCHQCLLRSVQEGLYGQGRNILGEVCSVRCLSSSASPPCAAFVPHELLLAILPPDVLHSLEDKTAAESLERSGLDLARCPFCAYAEVVALEEYRIKRSAKVALLASVLVLFSIVPLALMAFLILILTMMFTLRSPFLPRETSNSIQWILSLTACREHVEKSIGRIQRKRRGNFFRCGNARCGRDSCLECGREWAPFHKCYEKEEDSVRIYVEKAMADAIKRTCPVCHISFVKSDGCNKLTCPCGYVMCYVCRAEIGSESYKHFCQHFRQVPGTPCDECDRCDLYVQEDEAAAIDCAAEKAEAEYFRRFKKPTGWKYRRRDARAFGSRKGVKLSWPEAIDADLDRILDRVLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GED0</accession>
    <name>D5GED0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00001271001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430174">
        <property type="protein sequence ID" value="CAZ82873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838682.1">
        <property type="nucleotide sequence ID" value="XM_002838636.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GED0"/>
    <dbReference type="STRING" id="656061.D5GED0"/>
    <dbReference type="GeneID" id="9185529"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001271001"/>
    <dbReference type="eggNOG" id="KOG1485">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013430_2_2_1"/>
    <dbReference type="InParanoid" id="D5GED0"/>
    <dbReference type="OMA" id="CWALRNQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098771">
        <property type="term" value="P:inorganic ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030003">
        <property type="term" value="P:intracellular monoatomic cation homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1350:FF:000003">
        <property type="entry name" value="Cation diffusion facilitator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1510.10:FF:000005">
        <property type="entry name" value="Putative Cation diffusion facilitator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1350">
        <property type="entry name" value="Cation efflux protein, cytoplasmic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036837">
        <property type="entry name" value="Cation_efflux_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050291">
        <property type="entry name" value="CDF_Transporter"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43840:SF12">
        <property type="entry name" value="CATION DIFFUSION FACILITATOR 1 (AFU_ORTHOLOGUE AFUA_1G14440)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43840">
        <property type="entry name" value="MITOCHONDRIAL METAL TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160240">
        <property type="entry name" value="Cation efflux protein cytoplasmic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="129"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="157"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="200"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="242"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="302"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="8">
        <location>
            <begin position="136"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="50"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="63"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ82873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="423" mass="47072" checksum="983CB524D7429BD9" modified="2010-06-15" version="1">MAFTTSVTELHPLPAPTPNPPADPENLPTVSDPFSLSSGLKTSEEIHNLTARHRTARWKRPSAEQTLGESSTTGANNGGDNNKRMQKVDVKGIHEFYQSQNQSIQQLLKPVEEHRREAKDEKGDTRIRYLIAVNGSLAANIILAVLQLYGALSSGSLSLFATMADSIFDPCSNLLLILSHRAINKVDPNRFPSGKARIENAGNIVFCFLMCAVSLILIVVSIQELATHKAEDELNKFHLPSIIAVAVAFVTKLTLFLYCWGLRNIYSQVRILWEDHRNDLLINGFGILTSVGGSKLKWWLDPVGAIILSLLILGLWSRAAYREFLLLVGVTADRSLLRLITYISMTHSPLITQIDTVRAYHSGPRIIVEVDVVMSATTALQLTHDVAEELQVKLESLPDVERAYVHVDYETSHKPEHFLKKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEX3</accession>
    <name>D5GEX3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00006630001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430197">
        <property type="protein sequence ID" value="CAZ83066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838875.1">
        <property type="nucleotide sequence ID" value="XM_002838829.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEX3"/>
    <dbReference type="STRING" id="656061.D5GEX3"/>
    <dbReference type="GeneID" id="9188604"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006630001"/>
    <dbReference type="eggNOG" id="KOG3699">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006033_1_2_1"/>
    <dbReference type="InParanoid" id="D5GEX3"/>
    <dbReference type="OMA" id="EAVFWFV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.225.10:FF:000009">
        <property type="entry name" value="Class II aldolase/adducin N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="entry name" value="Class II aldolase/adducin N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051017">
        <property type="entry name" value="Aldolase-II_Adducin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001303">
        <property type="entry name" value="Aldolase_II/adducin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004855">
        <property type="entry name" value="PRK06208.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10672">
        <property type="entry name" value="ADDUCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10672:SF41">
        <property type="entry name" value="CLASS II ALDOLASE_ADDUCIN DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_3G01330)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00596">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01007">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="AraD/HMP-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Class II aldolase/adducin N-terminal" evidence="1">
        <location>
            <begin position="46"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="283" mass="30955" checksum="F0145689402779E7" modified="2010-06-15" version="1">MAPTVTSSYSALTAETTNVPIEDRIGIPEPGMEFESPEAERQYLKERLAAGFRIFAKHGFDEGVVGHISLRDPINPHAFWVNPLGLHFSLIKASDLLLIDESGKVLEGGANRHLNLAAFMTHSAIHKARPDVNCAAHAHSLYGRTFCTLGRTLDITTQDSCAFYNDHVLYGNFRGAVLDEGEGIDIAQTLGAKKAALLQNHGLLTTGGTVEETVFWFLSLEKCCHSQLLADAAAAGRGETTKKIGNKEAMETWKVFGSRRIGHFSGRPLFDLIEVETKGDYKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>D5GF42</accession>
    <name>D5GF42_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00001837001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430198">
        <property type="protein sequence ID" value="CAZ83135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838944.1">
        <property type="nucleotide sequence ID" value="XM_002838898.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GF42"/>
    <dbReference type="GeneID" id="9187996"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001837001"/>
    <dbReference type="eggNOG" id="ENOG502S29P">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1462361_0_0_1"/>
    <dbReference type="InParanoid" id="D5GF42"/>
    <dbReference type="OMA" id="VITVTEW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031342">
        <property type="entry name" value="Mug163-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17119">
        <property type="entry name" value="MMU163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="185" mass="20781" checksum="FD76E5777585A64A" modified="2010-06-15" version="1">MLKHSLRQSPLRRPLLLLRASISSNTPLLLYPPAPTPAPRKSSTHHHQPSYFAASIPNPSQPSKDHTPPSERSINLGRTIQTLKSHLPTLLQTPLPLDILSPSITLNLFPSTHPHLPAVRGRVAYVAALWTAPVAWGRLPGRHRRLEVLSERMLREGQGEGEEVFVVKWRMCEEEEFCGVFLFGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFA9</accession>
    <name>D5GFA9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00006805001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430208">
        <property type="protein sequence ID" value="CAZ83202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839011.1">
        <property type="nucleotide sequence ID" value="XM_002838965.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFA9"/>
    <dbReference type="STRING" id="656061.D5GFA9"/>
    <dbReference type="GeneID" id="9188058"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006805001"/>
    <dbReference type="eggNOG" id="KOG0075">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040729_10_3_1"/>
    <dbReference type="InParanoid" id="D5GFA9"/>
    <dbReference type="OMA" id="FRNMWER"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098852">
        <property type="term" value="C:lytic vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04159">
        <property type="entry name" value="Arl10_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001015">
        <property type="entry name" value="ADP-ribosylation factor-like protein 8B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044154">
        <property type="entry name" value="Arl8a/8b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45732">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45732:SF7">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SAR"/>
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    </dbReference>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="27"/>
            <end position="34"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="130"/>
            <end position="133"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ83202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="183" mass="20727" checksum="218934D2BE66C3D2" modified="2010-06-15" version="1">MSGFFRRIYDWLLRMFWATEMDVTMIGLQNAGKTSLLRVLAGGEFTYDSIPTVGFNMKRVQKGHVTLKCWDLGGQPRFRSMWERYCRGVSAIVFIVDSADDSSIPIAKDELHSLLHKPMLNGIPLLVLGNKSDLPDKIGVDELIEVMDLKDITNREVSCYSISAKEETNLDAVLQWLIGRAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFC6</accession>
    <name>D5GFC6_TUBMM</name>
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        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00006824001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="GeneID" id="9188075"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006824001"/>
    <dbReference type="eggNOG" id="KOG3362">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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    <dbReference type="OMA" id="CCMCGYW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="zf-HIT_ZNHIT1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039723">
        <property type="entry name" value="Vps71/ZNHIT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007529">
        <property type="entry name" value="Znf_HIT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13093">
        <property type="entry name" value="ZINC FINGER HIT DOMAIN CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04438">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51083">
        <property type="entry name" value="ZF_HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="HIT-type" evidence="6">
        <location>
            <begin position="172"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="29"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="46"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ83219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="209" mass="23067" checksum="8BDF7E2ABE45E026" modified="2010-06-15" version="1">MPTIQVLPNTSTSSGANWTYSNLPPALAHTNSLPSNTPRAPRAKRTNNIENSAARQRQINSRILDLERDNYRDATIVVGRSGVTGRSSSRNKSQNVRRILASQKTFANHLADEEALHPTPSAVHRQRVEEEDGMDFDGALKEGLINTQDPGQPLGYAASASRPSARPKRVFCEICGYWGKYKCQKCSARYCGLQCGGIHSETKCNKFYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFN7</accession>
    <name>D5GFN7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00007000001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430209">
        <property type="protein sequence ID" value="CAZ83330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839139.1">
        <property type="nucleotide sequence ID" value="XM_002839093.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFN7"/>
    <dbReference type="STRING" id="656061.D5GFN7"/>
    <dbReference type="GeneID" id="9188278"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007000001"/>
    <dbReference type="eggNOG" id="KOG0598">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_63_43_1"/>
    <dbReference type="InParanoid" id="D5GFN7"/>
    <dbReference type="OMA" id="CEVDWWS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004703">
        <property type="term" value="F:G protein-coupled receptor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05578">
        <property type="entry name" value="STKc_Yank1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000354">
        <property type="entry name" value="AGC/YANK protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000469">
        <property type="entry name" value="Serine/threonine-protein kinase 32B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24355">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR KINASE/RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24355:SF30">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE 32B ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="20"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="9">
        <location>
            <begin position="277"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="360"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="470"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="423"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="505"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ83330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="521" mass="58369" checksum="09619BBDD3F2611D" modified="2010-06-15" version="1">MGNSSGKPVVFTEEVNLNHFRLLRVVGKGAFGKVRIVERRDTGLTFALKYIRKDEVVRSESVRNIIRERRMLEQLNHPFLCNLRYSFQDIEYLYLVVDLMNGGDLRFHISRKTFTEEAVRFWISELACALLYIHGRGIVHRDVKPDNILLDSDGHVHLADFNVASDIHPDRPLSSKSGTLAYLAPEVYSGNGYDTGVDWWSLGVVFYECIYGKRPFTSDTHETLGKEIMKASPKYAVTKPPVTRDCMDAIRSLLQPDVTKRIGATGFNTFTSHPFFADIDFDALERKEYDPVFKPSTEKTNFDATYDLEELLLEEAPLEARARRQRPRERLKDDATAQEIRQEELHRMIETLFEPFDYTTAGFDSPTKGGRHPGTVDPASKSAGEPLDWSQKSLVGSPASGSPPIPSCTDLPKVCPPPRSMPPTVSSTDRSNMKEQQLQKRQSIGKLRSGMRNQSSSGGVQVVLDEAGSWSDLADKSSPTPVRLKPSGMLGFFSKRKGRASSPKPQERGVLGREGARVAIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFY5</accession>
    <name>D5GFY5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00001950001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LTV1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430227">
        <property type="protein sequence ID" value="CAZ83428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839237.1">
        <property type="nucleotide sequence ID" value="XM_002839191.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFY5"/>
    <dbReference type="FunCoup" id="D5GFY5">
        <property type="interactions" value="419"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GFY5"/>
    <dbReference type="GeneID" id="9181773"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001950001"/>
    <dbReference type="eggNOG" id="KOG2637">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028555_1_0_1"/>
    <dbReference type="InParanoid" id="D5GFY5"/>
    <dbReference type="OMA" id="TAQHFTL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007307">
        <property type="entry name" value="Ltv1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21531">
        <property type="entry name" value="LOW-TEMPERATURE VIABILITY PROTEIN LTV1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21531:SF0">
        <property type="entry name" value="PROTEIN LTV1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04180">
        <property type="entry name" value="LTV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="196"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="252"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="331"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="206"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="271"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="331"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ83428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="404" mass="45145" checksum="42CDD57400F2A5F9" modified="2010-06-15" version="1">MPTKKWIDKKTATNYQLRYRAQTDPLVHDDQASELVFTQVTAPNASRSIPALTASSSKIKTTTDLAQELSPPSTSLRKNEGEAAIHGVYYDDTEYDYMQHMRDINDEQVYFVDVQEKSSQGQNKKKNKMKLEDALRLPQEVFPSVVEVKRSYQDMQDVPDALSGFQPDMDPRLREVLEALEDEAYVDDDEDVFGELAKGGEISPGEFEEGEEDGWESDVTEKPSNNPPPAGKEGKEGEGEDWCRAFKAFKNDQRKKRGGKVAFDNDSLADTMSFGGNSTTSSFGGTMSVSNRTRRRKKKAGTESSGYSMSSSALFRTEGLTLLDDRFDKIEEEYAEEEEGEDDLRSNSGVALPETRRDLDSVLDEFLDGYSVGKKGRVRRGKYGSGMEQLDEIRRGLGGARIAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFZ8</accession>
    <name>D5GFZ8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00007130001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430231">
        <property type="protein sequence ID" value="CAZ83441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839250.1">
        <property type="nucleotide sequence ID" value="XM_002839204.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFZ8"/>
    <dbReference type="STRING" id="656061.D5GFZ8"/>
    <dbReference type="GeneID" id="9181810"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007130001"/>
    <dbReference type="HOGENOM" id="CLU_2727897_0_0_1"/>
    <dbReference type="InParanoid" id="D5GFZ8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009215">
        <property type="entry name" value="TIM-br_IGPS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051353">
        <property type="entry name" value="Tobamovirus_resist_UPF0261"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31862">
        <property type="entry name" value="UPF0261 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_1G10120)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31862:SF1">
        <property type="entry name" value="UPF0261 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_1G10120)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09370">
        <property type="entry name" value="PEP_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TIM-barrel" evidence="2">
        <location>
            <begin position="16"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ83441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="66" mass="6924" checksum="A176CE18071BB832" modified="2010-06-15" version="1">MTARMQRPPATRAEALQRPRDTLASGGTIIRAGAGIGPTAKPTEAGGADLIIIYNSGRYRMAGRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGD1</accession>
    <name>D5GGD1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007321001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430264">
        <property type="protein sequence ID" value="CAZ83574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839383.1">
        <property type="nucleotide sequence ID" value="XM_002839337.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGD1"/>
    <dbReference type="FunCoup" id="D5GGD1">
        <property type="interactions" value="125"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GGD1"/>
    <dbReference type="GeneID" id="9183172"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007321001"/>
    <dbReference type="eggNOG" id="ENOG502S0A4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089054_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGD1"/>
    <dbReference type="OMA" id="CSQFFVG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024388">
        <property type="entry name" value="Ribosomal_mL58"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28266">
        <property type="entry name" value="54S RIBOSOMAL PROTEIN L20, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28266:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12824">
        <property type="entry name" value="MRP-L20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="180"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="203" mass="23233" checksum="45814BE984CF8978" modified="2010-06-15" version="1">MFNPATFLRRGLPEIRSTTSKSLAFIRHESNTRRHTKRLRLPPHPDFKTHLTTDSPSLTQILHNPPAAAPSVFVTPTLFLPKDDPRRALQTAASPINGTPEELTLPPPVTEIKEKKYHLTPTDFSRMRSLRKSDPGLWTRRKLAEEFGTSSLFVGMVAEADDERRKKMWAKAEMVKKQWGPRRRNARIDRAKRREGWGGADGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGF5</accession>
    <name>D5GGF5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00007355001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430264">
        <property type="protein sequence ID" value="CAZ83598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839407.1">
        <property type="nucleotide sequence ID" value="XM_002839361.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGF5"/>
    <dbReference type="STRING" id="656061.D5GGF5"/>
    <dbReference type="GeneID" id="9183196"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007355001"/>
    <dbReference type="eggNOG" id="ENOG502QS1V">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056435_7_0_1"/>
    <dbReference type="InParanoid" id="D5GGF5"/>
    <dbReference type="OMA" id="FFKSDWE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008854">
        <property type="entry name" value="TPMT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32183">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32183:SF6">
        <property type="entry name" value="CYSTEINE SULFINATE DESULFINASE_CYSTEINE DESULFURASE AND RELATED ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05724">
        <property type="entry name" value="TPMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51585">
        <property type="entry name" value="SAM_MT_TPMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ83598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="267" mass="30698" checksum="B46979991A00E826" modified="2010-06-15" version="1">MTNVGASMDHNWPTDERQRLREIFSVSTDRHQEKWAELWDNGEFIPWDKGCPSPALVDLLVQRQQHTSFTAQDLVARGERRKRALVPGCGRGYDVFLLATHGYDAFGVEISKTAVKEAREWASDQLEIEKAASGQTLETDRWGRIQMVEGDFFKDDWIEAMEIDLRGGFDLVYDYTFLCALHPTLRRSWAVRMAELLAPQRGLLVCLEFPLYKPIETGGPPWGLKNEVYDELLGGPMGAFQKVLHYKPERTHKIGEGTDWISVWRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGH4</accession>
    <name>D5GGH4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007385001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430264">
        <property type="protein sequence ID" value="CAZ83617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839426.1">
        <property type="nucleotide sequence ID" value="XM_002839380.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGH4"/>
    <dbReference type="STRING" id="656061.D5GGH4"/>
    <dbReference type="GeneID" id="9183215"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007385001"/>
    <dbReference type="eggNOG" id="ENOG502QPI4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015126_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGH4"/>
    <dbReference type="OMA" id="HKTLDKQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42345">
        <property type="entry name" value="TPR_REGION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42345:SF1">
        <property type="entry name" value="VTC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="610"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="615"/>
            <end position="624"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="711" mass="79264" checksum="791B44062A979BA3" modified="2010-06-15" version="1">MPISVSPSPSPPLQLCLPAAAVPQHNVRAIRAPPREWPPIRYQTELSATEAKKFIGDGAPKFCGELREITNGPRGYYYLFAFAGDEWDEESDTALFQSDSFCDMSSSPEGRQTDALERPAMAHAFGIYPGTITLSCYVTSFGSTLQGHEVAARGKVRKLTMLYVLDRLRILQVKGIEEDLGQLHMHLYDYLLHDTHRYDSKNHVSLDNQISDLVAALCRPSWVNFSSSENQTIARFLSDPNPGVANSFFHQLLLSIELYLRINARHQTNLAAKPLPDMPEKIRWDLMLAQRWLENVEVEAPKKVKDGKGKREQKSSVGFKFPHKKTQVEALRNFAWTLKWPNMHEVEYTLSEGEDPNEVLEDRSINCMSWFTGLLLPGKSMPWILMNALTDCDPDVPETFHNLSQTTPNVGFQYRGHTYWSWECIVGKVLGAAAGVAQIAGWIGPCSASADLSRSEMAIINQMPPRGNKITPSNVRGMALNSDPLGACQTTYPVDDYVLLTPSTDDPVEDIRIERLNFSPAASSPKKGVPRITENGHQAAEESPVLTFDASITFAITVERRTRSWKVSLRHNTPFIAAYPCQNGPHVLFCEYQHRVIYADKLVEIQNWGPSGGVEDSEVDDEGGQPENTSTGVEEVLVVEAFGVPDNEVFVRAWCSFWGLPAITADVNKTCMACAIREAYAALVSVVILTNKSSISVEVEEVDRLMENLQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGR0</accession>
    <name>D5GGR0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00007478001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430275">
        <property type="protein sequence ID" value="CAZ83682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839491.1">
        <property type="nucleotide sequence ID" value="XM_002839445.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGR0"/>
    <dbReference type="STRING" id="656061.D5GGR0"/>
    <dbReference type="GeneID" id="9185604"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007478001"/>
    <dbReference type="eggNOG" id="KOG2128">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009455_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGR0"/>
    <dbReference type="OMA" id="AYMITLN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046580">
        <property type="term" value="P:negative regulation of Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05132">
        <property type="entry name" value="RasGAP_GAPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000593">
        <property type="entry name" value="RasGAP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023152">
        <property type="entry name" value="RasGAP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001936">
        <property type="entry name" value="RasGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14149:SF17">
        <property type="entry name" value="GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14149">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEIN WITH IQ MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00616">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03836">
        <property type="entry name" value="RasGAP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00323">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143885">
        <property type="entry name" value="RGC domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00509">
        <property type="entry name" value="RAS_GTPASE_ACTIV_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50018">
        <property type="entry name" value="RAS_GTPASE_ACTIV_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-GAP" evidence="3">
        <location>
            <begin position="188"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="61"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="22"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ83682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="782" mass="89566" checksum="F6DB06D3CE23D3FD" modified="2010-06-15" version="1">MSVTTMLAPSARNSTSSTSSSFVPVTRQNTIGSDGAPRSVRASKRYSVTALYLSMSAQDKDMEIEDELARVQKTLRDLKSRISSQSKKNFVLEKDVRYLDSRIALLIQNRMALEEQNEVASHFDESSELQEGTFPDDRKTQQYGNLFFLLQSEPRHIAALCRLVNMAEIDSLLQTVMFTIYGNQYESREEHLLLTMFQSVLSAQFDTTPEYSSLLRANTPVSRMMTTYTRRGPGQSYLKVVLAKQINNLIELKDLNLEINPLKVYERMVKNVEEDTGTLPANMPRSVSAEEAAENPQVQAIIEPRLTMLMELANTFLSTIIDNINETPYGIRWICKQIRSLTRRKYPDAKDNIICTLIGGFFFLRFLNPAIVTPRSYMLIDNIPSEHPRRTLTLIAKMLQNLANKPSYSKEPYMAKLSPFVTANKNRISKFLNDLCEVGDFYESLEMDNYVALSKKDLELNITLNEVYATHNLLEKHAKELLQGRDTEHLTILLQELGPAPPQVPRKENRAIILPLFSRWESALDDLSAALDITQEDVYFMEAKSIFVQLLRTLPPISAAVRRPLNLKKIADVAATTKDIGTFRKGIRGLELLNQLKDLGVISEVDEYQILREEVEQELVHLGSLKEKVTEESLKLDEVYKTIRDHNAYLVSQLETYKSYLHNVRSQSEGKQRGKQQKQHVLGPYKFTHQQLEKEGVIQKSNVPDNRRANIFFTITSPTPGTFVISLHYKGRNRGLLELDLKLDDLLEMQQNNHDELDLEYVQFNVPKVLALLNKRFARKKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGU6</accession>
    <name>D5GGU6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00007518001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430275">
        <property type="protein sequence ID" value="CAZ83718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839527.1">
        <property type="nucleotide sequence ID" value="XM_002839481.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGU6"/>
    <dbReference type="FunCoup" id="D5GGU6">
        <property type="interactions" value="133"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GGU6"/>
    <dbReference type="GeneID" id="9181688"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007518001"/>
    <dbReference type="eggNOG" id="KOG1983">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006030_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGU6"/>
    <dbReference type="OMA" id="QIYVFGQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045159">
        <property type="term" value="F:myosin II binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013905">
        <property type="entry name" value="Lgl_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10241">
        <property type="entry name" value="LETHAL 2 GIANT LARVAE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10241:SF25">
        <property type="entry name" value="TOMOSYN, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08596">
        <property type="entry name" value="Lgl_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lethal giant larvae (Lgl)-like C-terminal" evidence="2">
        <location>
            <begin position="520"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="557"/>
            <end position="586"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ83718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1005" mass="110127" checksum="845FBCB66F5DA248" modified="2010-06-15" version="1">MDFLRSKQAGIQHDFTGALAAPDLFVLDEVEKYGIESSVSVLAYDPIQSLFAVGTNETATTPGKVYVFGQKRVQVTFTLTRKAGVKFLKLCDNRIVVVDTKSELSVFDMTDLEAQPARYSPPGIVSAVLTDPSLDWVFMGLQNGEVVAYDLDRRLRSPFLVPNLWRERSPKARVLPIVSLALHPRDLGTLLIGYLEGCVVFSLKQHAPTLHLQFEIPPGAPGADPERSMLNSVRRPRLCEALWHPTGTFICTTHEDSCLVIWDSRDGRVVQARTLQDTNVNIPCGPQPSMLSETEGTIIVRQPLFKVSWCCTDNPDDTSILVAGGNLSVMPNKGLTLLDLGPTPNMLTSSIQFVSDHFASPRRQRVLPTPADLDVVDFCLLPRTSPHYAGSHDPVAVIALLASGELVTLSFPDGQPISPALVFHPSLSLIHPRVTTANLSPINRQRWLGMIESRDGPKEVIIGGVEHVKPLRRYESRTVLQMAHLDGTVKIWDLGLADEIENEKIMEVDLGQVLERGVDLKIECVSMAGFTGELAVGMETGEVVLFRWGKNKGYGRTREDEALQSMSRSEPGSPTGAIRDAKSKSNPTLKEGLLPLCVLEQHCGSVLHLACSDVGFVAIAYQTGHVSVVDLRGPAVIFHQSLTSLTKEAKRSSVRKSHDKPMEGEKATCLEFGVLTLEGDGKQLITNTLEHLLTVNHRVLKHCTVRPGFVCNFVNITHMEGPVIKIIPLNSDSGKRVYATQQAVANLRDGIKVPGVLLGVTKSESRIFRPPTAKGAHKSWEDSDCLSANVVELETHGVCLSCVMGNGVVKSYSIPGLKEISEVKIGQWFEKSRLADIIVTGAGYIFGWTSEAECSLLHMWGKGDSWRVDTLYNPNTPVPARPTISNFQWISGTQYVTVEDVDLLIGGPERPMSRKMIEQMRAEKRQAEIDARTGSAAAAAGASNQDGVFANMAKQIQERTEKLNIMGDSMDRLGESSANFAEDVGKFVNQQKRKALLGGLTGKWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGW8</accession>
    <name>D5GGW8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007555001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430286">
        <property type="protein sequence ID" value="CAZ83761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839570.1">
        <property type="nucleotide sequence ID" value="XM_002839524.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGW8"/>
    <dbReference type="STRING" id="656061.D5GGW8"/>
    <dbReference type="GeneID" id="9181732"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007555001"/>
    <dbReference type="HOGENOM" id="CLU_1267720_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGW8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="218" mass="23079" checksum="6A746E70A3D32263" modified="2010-06-15" version="1">MQARRATRQRSSSNIDNNDENRGTAGVSTRLTRAKAAALTSSDSATSLPAKTTTTGALPANRKRSALGDVSNVVKADGVDDKKAGGKKGGKPALSTKTVNGVTKQTRAKAQPIKSVAPEETKRTNSGSGVLAARKKKAATGVPGAGSAKAKEDRENEVESAKVTISSVEIEVPAKSETEVVRATKKPRVEERLAHNDLDEEDHLRIPTWTGNYYYAQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGZ4</accession>
    <name>D5GGZ4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00007630001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430297">
        <property type="protein sequence ID" value="CAZ83787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839596.1">
        <property type="nucleotide sequence ID" value="XM_002839550.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGZ4"/>
    <dbReference type="STRING" id="656061.D5GGZ4"/>
    <dbReference type="GeneID" id="9185041"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007630001"/>
    <dbReference type="eggNOG" id="KOG2425">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_767665_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGZ4"/>
    <dbReference type="OMA" id="YSTAICR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090730">
        <property type="term" value="C:Las1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000460">
        <property type="term" value="P:maturation of 5.8S rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000470">
        <property type="term" value="P:maturation of LSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007174">
        <property type="entry name" value="Las1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15002">
        <property type="entry name" value="RIBOSOMAL BIOGENESIS PROTEIN LAS1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15002:SF0">
        <property type="entry name" value="RIBOSOMAL BIOGENESIS PROTEIN LAS1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04031">
        <property type="entry name" value="Las1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ83787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="361" mass="39556" checksum="737D8FD1B5F2DDCC" modified="2010-06-15" version="1">MHTLAKTLGLPTSFVEIRHAATHEALPSLPLLRTATTRALDWLWENYWCSLSDSDSDPEPAEEGGIKARALLKSFRTLRRAEPTRALRSGDGREETKLTMGLIKECICIAGDGLVQALLEEKALIPAGKHKPSSMKGAILLWTPLLDALDAAVLGFGNSLLLAMLESLKRVAAGGQQCEFAEAVMAWLVHLTTSNAGAKSGFGRNPDAPVDLDGLAKQCVLYPSKWTMRLLRHLLKEHAALRGKYAGLVEMAGAQVLLSRDLAVEIEEGMKKRASKAYGEEENTQFTGKKRSIHDVEVEVEMFEARYEAMKKMRLSGNSESAITTSILEGEGPTGREEGEVVIGRWKKWSGKWVPRPIGVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5GH03</accession>
    <name>D5GH03_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00007640001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430297">
        <property type="protein sequence ID" value="CAZ83796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839605.1">
        <property type="nucleotide sequence ID" value="XM_002839559.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GH03"/>
    <dbReference type="FunCoup" id="D5GH03">
        <property type="interactions" value="20"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GH03"/>
    <dbReference type="GeneID" id="9185050"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007640001"/>
    <dbReference type="eggNOG" id="ENOG502QS9U">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026015_1_0_1"/>
    <dbReference type="InParanoid" id="D5GH03"/>
    <dbReference type="OMA" id="MVVAEEW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070086">
        <property type="term" value="P:ubiquitin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050357">
        <property type="entry name" value="Arrestin_domain-protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024391">
        <property type="entry name" value="LDB19_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188:SF76">
        <property type="entry name" value="PROTEIN LDB19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13002">
        <property type="entry name" value="LDB19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LDB19 N-terminal" evidence="2">
        <location>
            <begin position="115"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ83796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="440" mass="47877" checksum="1DDA5D2B77B07CD2" modified="2010-06-15" version="1">MPVIGFFHHGASPSTSMTSILPIDSKHPAPKRRWSSNRSSSESDRPKLTIRTMSRGSSSRGGLKGFIENPADITVAIESPPLVCYGPPKESTGALLSGIMKLQVKETEQTFEAFTMRLLADVLTKKPVGAHCSDCTTSVSELHKWQLISEPVVLKKGTHTYPFSYLLPGHLPASNNNALSRITYCLYATATTMKGDEIKFEKPITLQRAVLPGPDRHSTRVFPPTDLSANVTLPPVAHPGGDFPLHIRLDGVLSKARTSRWRLKRLSWRIDETSRVVSPACKQHSGKLGGDGKGLLHDDVRTVGSGEVKSGWKSDFNSTDGSIEVEFNARIHAHADAACDVDSPCGISVSHNLVVEMIVAEEHCPVPPSRVANPTGAARVLRMQFHLIVTERSGLGISWDEEIPPVYENIPASPPNYGTVLEVEGEEGVALFCPPVYQES</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GH17</accession>
    <name>D5GH17_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">DNA polymerase alpha subunit B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00007656001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Accessory subunit of the DNA polymerase alpha complex (also known as the alpha DNA polymerase-primase complex) which plays an essential role in the initiation of DNA synthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the DNA polymerase alpha subunit B family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430297">
        <property type="protein sequence ID" value="CAZ83810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839619.1">
        <property type="nucleotide sequence ID" value="XM_002839573.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GH17"/>
    <dbReference type="FunCoup" id="D5GH17">
        <property type="interactions" value="398"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GH17"/>
    <dbReference type="GeneID" id="9185064"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007656001"/>
    <dbReference type="eggNOG" id="KOG1625">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014923_1_0_1"/>
    <dbReference type="InParanoid" id="D5GH17"/>
    <dbReference type="OMA" id="PFLDIEH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005658">
        <property type="term" value="C:alpha DNA polymerase:primase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016233">
        <property type="term" value="P:telomere capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007185">
        <property type="entry name" value="DNA_pol_a/d/e_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016722">
        <property type="entry name" value="DNA_pol_alpha_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054300">
        <property type="entry name" value="OB_DPOA2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23061">
        <property type="entry name" value="DNA POLYMERASE 2 ALPHA 70 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23061:SF12">
        <property type="entry name" value="DNA POLYMERASE ALPHA SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04042">
        <property type="entry name" value="DNA_pol_E_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22062">
        <property type="entry name" value="OB_DPOA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018300">
        <property type="entry name" value="DNA_pol_alph_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA polymerase alpha subunit B OB" evidence="8">
        <location>
            <begin position="237"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="DNA polymerase alpha/delta/epsilon subunit B" evidence="7">
        <location>
            <begin position="372"/>
            <end position="593"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR018300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF22062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ83810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="652" mass="71664" checksum="65386EDB06E0117F" modified="2010-06-15" version="1">MDHSSTSETIAEKFGKLTPEVLGELESMLRIYGIGAEELFYKWESYCIKMGSEDTKLNLDTVLVFKKDVQELFGKEIKSKKRMHVANTSRPVARTPRVKATGDHVGFLDVLVPTTPRGEKGSVKRKFEKAPFETPSSKAGRFLDVNSPGAAIGSSPVGTPHKAGGQQIPSTPFAQRKNAGSTVEVLNEHIPKPSLPLPESSADAELASWNSRVKVVSIVELKKFSYRPMHQKLSEASEVMDDRIDEFMDMIQEHHKLADQQFGNPSTASPSEIIAVGRISCDTLDGKLSPSSVLLEASRKMGAGSRVRLKLDAVLSYSFFPGQIAAVRGVNASGEYLAVREILDIPRLPPPTSSPNVLLETANRLSAGGLNIVVASGPYTTDDNLHFEALSEICNKAAESCPDVLILTGPFIDADHPLIRTGDFDFEIPDREYGDGGSMEDLFRERISPKIRLVQRSMVLLVPSVRDVVSGHISFPQEQFKRRQLELPNVRCLPNPATFSINEIVFSVSTNDILFHMTSQEISRNPQDTNPTARFSKALIYQRNLYPLFPGPSKEQLPRGATGPNLDVPNLRLADLVSLAPDVLVLPSMLASSAKVVDGVLVLNPGFLSKRMGPGSYAEMHVLPPQVKDGEEGEEELSHRVWERARVEVLRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>D5GH25</accession>
    <name>D5GH25_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Serine/threonine-protein kinase TEL1</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">ATM homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21 22">DNA-damage checkpoint kinase TEL1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Serine/threonine-protein kinase tel1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Telomere length regulation protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="ORF">GSTUM_00007669001</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27 28">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates histone H2A to form H2AS128ph (gamma-H2A) at sites of DNA damage, involved in the regulation of DNA damage response mechanism. Required for the control of telomere length and genome stability.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Associates with DNA double-strand breaks.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PI3/PI4-kinase family. ATM subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="EMBL" id="FN430297">
        <property type="protein sequence ID" value="CAZ83818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839627.1">
        <property type="nucleotide sequence ID" value="XM_002839581.1"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GH25"/>
    <dbReference type="GeneID" id="9185072"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007669001"/>
    <dbReference type="eggNOG" id="KOG0892">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000178_8_0_1"/>
    <dbReference type="InParanoid" id="D5GH25"/>
    <dbReference type="OMA" id="LRICMDH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05171">
        <property type="entry name" value="PIKKc_ATM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1010.10:FF:000019">
        <property type="entry name" value="Serine/threonine-protein kinase Tel1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038980">
        <property type="entry name" value="ATM_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014009">
        <property type="entry name" value="PIK_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044107">
        <property type="entry name" value="PIKKc_ATM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37079">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE ATM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37079:SF4">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE ATM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51189">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="8" id="KW-0158">Chromosome</keyword>
    <keyword evidence="12" id="KW-0227">DNA damage</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="16" id="KW-0779">Telomere</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FAT" evidence="26">
        <location>
            <begin position="616"/>
            <end position="1210"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="25">
        <location>
            <begin position="1314"/>
            <end position="1631"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00014619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00020288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00025079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00030020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00030222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00031460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00032467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS51189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EMBL" id="CAZ83818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1684" mass="188901" checksum="D8C62317E5D9E647" modified="2010-06-15" version="1">MKEKDDASQELAKILDVPYDDLLEKGFHKILAYGVAGAMATPPRPDAPQPPNVELRIRQRLGDEEYKRLLEKKFALIIASLFQVMQEDGTSDRFLSKDPSLAATAAYMRDIVEVSHSTGKIPDPLRPYFKVKVVLNAVHHLCGRLQYDDTRLWTPAMFTFVTRRLFDTMDPALGSLSACAVVRKIRLLVCLANPKSIDGYALEMVIHGLKPYIVDTYCAEDAVGVVQYLLDQGKGYLSTQPAFVIGTFLSILASLRAFLSAATGQEDTQVQLSLNIAQSFHIWLGDYLTGCHFPELNEMQNQLFQAIVESAMGFRLHGNASSGTKESDLLRHLLDDDRNKNKLLDDVSRGLAFSLVCSGFQKPVSFRDDIFGSDEESVARSRVSYAASGDIHSEWTQETELEKITDLDTTQYDLDIIPKAGIFQLLLKLLLSDDRLVIGLAEATLETIFFDESRSPGVATFHYILDENMYKAMKWSILPMSLSENENPRPITSVTKPEILPVDEWVKDLTTAISTNLQEDAVVKNLRPILEKVGGLAKDLFPFVVHILLLHHIKGDDELKMELSELFRDCFRTCSEKTVPHSIILIKTILYLRTQETAQEATKLPRDHWLDVDYLEMARAACICKMFKTSLLFAEIYHSESGNAEFPDSKLLLEIFRNVDDLDSYYGVSQASSLETVLKRFEYEEDGWKSLSFRGANLESHLRLGSTSDGQDLFGVVDAFNTLGMNGLSHAFLQSGTAADASEDTLDNMFRSAWKLEQWDLPCPSSCTTRSATIYRALQSVNNNLDMRAIPSQLDSSFFGVMRQITAGKQTGHSLGASMRTLAMLTEMEEVLVSKETGELEQVWERLQNRNSWMHIGRYSDVEEIMTMRQTTLSSVSKRDHLRRAAGVDIKTARFLEAKALVASCKMARSHKVLQHSLSAATQLSKAVEPCKQVGLDINAVATLQAANVLWDHGQGIASIRMLQALGFDNALGQQSIVVGKAKLLAKLGNWVSEARLEKPDKIMSNYLVFAIDELKENYSGSEAGRVFHEFASFCDQQLQNSGNIEDYQRALKLRQNKEAEVRELEKLMKSAAGGKLEQLTLYRHKAKTWLNLDDAEFNRLRLDREAFLERSISNYLRALAACDDYDHDAVRFCALWLEHSGDAKVNKAAEAPLGQVASRKFVPLMNQLSSRLLESPEDIFQSLLFPLILRICMDHPHHGHYQILALTRAKPKDNISQSRQASAAKIATKLKAHPSSKPLFSHIIQATTSYIKLATQKIERKPGIKPVLRSVLTRDIYSKFSVDIPNWKIPPPTMQIDVRADKDYSGLPVIAKFHPEITIASGISAPKIVTCLASDGRTFKMLFKGGNDDLRQDAIMEQVFEQVSNLLKKSRTTRQRNLSIRNYKVLPLATNAGIIEFVANTIPLYDFLMPAHMSYHPRDWKPTYCRKTITDVQTKTREVRIDAFQRVAAHFRPVMRYFFMHKFNGPDDWFSSRLAYSRSTAAISILGHVLGLGDRHGHNILLDEKSGEVVHIDLGVAFEQGRILPVPEVVPFRLTRDIIDGFGVTRTEGVFRRSCEFTLTVLRNEAYNITTILDVLRYDPLYSWTISPLRMKKMQDQVEAAATGIVDEGIVGTGGAGKKDEESEADRALTVVAKKLSATLSVGATVNELIQVAGDERNLAVLYSGEYLFFLVSPISLMVGGWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5GH62</accession>
    <name>D5GH62_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Conserved oligomeric Golgi complex subunit 8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Component of oligomeric Golgi complex 8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00007764001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COG8 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430308">
        <property type="protein sequence ID" value="CAZ83887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839696.1">
        <property type="nucleotide sequence ID" value="XM_002839650.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GH62"/>
    <dbReference type="STRING" id="656061.D5GH62"/>
    <dbReference type="GeneID" id="9182592"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007764001"/>
    <dbReference type="eggNOG" id="KOG2069">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025395_1_0_1"/>
    <dbReference type="InParanoid" id="D5GH62"/>
    <dbReference type="OMA" id="DAMKDMT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017119">
        <property type="term" value="C:Golgi transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007255">
        <property type="entry name" value="COG8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21311">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX COMPONENT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21311:SF0">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04124">
        <property type="entry name" value="Dor1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="263"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="330"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="263"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="277"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="331"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="344"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ83887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="483" mass="52274" checksum="2B0D3707EFF07AD4" modified="2010-06-15" version="1">MDPSVSSFNPLLDLLLPHIPQSAALPDPAVSTQYFSRISSLALTSIVDSEPASLSSSLHSLNLSLQSLSSRSHKSIIAASNHLSAVPQSIQSLHSSLTTLENSLPAIDSEITSFTTNFSRDGTYLTNRSRTHLLSKNLDRLLDILQLPALLQSLISTSSYPTALDLLAHVRRLHSLYHDSPTIDSVYAECETLQKSMTANLISTLRGPLKLPTAMKTIGFLRRCGTKEAVMDERGLRGLFLVCRLAFLSGLLSALDPLKELAEEERERRQNGGDENTSSTDTTTTTTPSSWGGGTQTERYLKRWIEVFREQSFGIVSMYKSIFPNALPAANPDPPPPSSPHPHSPIMSSVGIGIRSRSSSTLSNMSSSANDDDPEGGQAPDPLASFVLHLLSLLTETLGRYLKTIEERSVRESLLTQVLYAAGSMGRLGGEFGAILGVIECFGMGDGEREEGKDGDGEEEEWVGVVAKHKVLASRLETLAGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHG6</accession>
    <name>D5GHG6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00007860001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430318">
        <property type="protein sequence ID" value="CAZ83959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839768.1">
        <property type="nucleotide sequence ID" value="XM_002839722.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHG6"/>
    <dbReference type="STRING" id="656061.D5GHG6"/>
    <dbReference type="GeneID" id="9183604"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007860001"/>
    <dbReference type="eggNOG" id="ENOG502RYAY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010112_0_1_1"/>
    <dbReference type="InParanoid" id="D5GHG6"/>
    <dbReference type="OMA" id="PIMFTIC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046623">
        <property type="entry name" value="DUF6536"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35395">
        <property type="entry name" value="DUF6536 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35395:SF1">
        <property type="entry name" value="DUF6536 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20163">
        <property type="entry name" value="DUF6536"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="19"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="227"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="318"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="363"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="389"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="441"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="482"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="DUF6536" evidence="2">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ83959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="584" mass="65110" checksum="26B9E9EB669E06C0" modified="2010-06-15" version="1">MDIGVPSVRNLFGIKLERVFLWIAIGITSIPLHLLYNSAIYTSLAANEFFVTIVTNNHFESGASSNMTEDLFSMYRENYNITNWTGDMSQKFHQLRIVLEEYNTSAQNYDDLTPRRCTKLYNTEFISSHRNLFLITKQSDNAMHNTTLLDTFQVGVNGISPGRWMCDYHGVASGETYYQDGPTCNPSDLVSNVTNGLPWLLQLRRGGEVEISGCKSEKTEEKCKVQFSLRIMIVVICCNIAKACCMIITVVRSREPTLVTLGDAVDSFLRIPDPTTAAICFADRQFVKREWGCGWRTRPRQWRKMGVQKWWTSVSKTRWITCNALCSVAIMAAGVSLASGMGKDGRYWSNDIKSMWTRGFGKVNSVSLVGISFKNITMAILLANVPQTILSFLYLTYHSLFTCMLLGHEWSLFGHHHRTLRVTSPRPGQCSTYWLQMPYNYAIPLMVLSGLLHWLASQSIFLARVEIRDPLERPTPATISTIGYSCIAIIFALTLGILALLAAIGMGYRPLASEITAAGSCSAAISAACHTWEDPEVIVGKKVRWGDVGIVPNLVVRHLTFSSEGGVGKPVFGGIYAGTGQDRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHQ8</accession>
    <name>D5GHQ8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Alanine--tRNA ligase</fullName>
            <ecNumber evidence="15">6.1.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Alanyl-tRNA synthetase</fullName>
            <shortName evidence="15">AlaRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ALA1</name>
        <name evidence="18" type="ORF">GSTUM_00007997001</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14 15">Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 15">
            <text>tRNA(Ala) + L-alanine + ATP = L-alanyl-tRNA(Ala) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:12540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:9923"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78497"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="15">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="15">Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. Alax-L subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.7" evidence="15"/>
    <dbReference type="EMBL" id="FN430320">
        <property type="protein sequence ID" value="CAZ84019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839828.1">
        <property type="nucleotide sequence ID" value="XM_002839782.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHQ8"/>
    <dbReference type="FunCoup" id="D5GHQ8">
        <property type="interactions" value="956"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GHQ8"/>
    <dbReference type="GeneID" id="9183673"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007997001"/>
    <dbReference type="eggNOG" id="KOG0188">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004485_5_0_1"/>
    <dbReference type="InParanoid" id="D5GHQ8"/>
    <dbReference type="OMA" id="NKKDNFW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004813">
        <property type="term" value="F:alanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070143">
        <property type="term" value="P:mitochondrial alanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00673">
        <property type="entry name" value="AlaRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.130:FF:000004">
        <property type="entry name" value="Alanine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.310.40:FF:000003">
        <property type="entry name" value="Alanine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000011">
        <property type="entry name" value="Alanine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.980.10:FF:000004">
        <property type="entry name" value="Alanine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.980.10">
        <property type="entry name" value="Threonyl-trna Synthetase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00036_B">
        <property type="entry name" value="Ala_tRNA_synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002318">
        <property type="entry name" value="Ala-tRNA-lgiase_IIc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018162">
        <property type="entry name" value="Ala-tRNA-ligase_IIc_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018165">
        <property type="entry name" value="Ala-tRNA-synth_IIc_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018164">
        <property type="entry name" value="Ala-tRNA-synth_IIc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050058">
        <property type="entry name" value="Ala-tRNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059090">
        <property type="entry name" value="ALA1_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023033">
        <property type="entry name" value="Ala_tRNA_ligase_euk/bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00344">
        <property type="entry name" value="alaS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11777:SF9">
        <property type="entry name" value="ALANINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11777">
        <property type="entry name" value="ALANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26023">
        <property type="entry name" value="ALA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01411">
        <property type="entry name" value="tRNA-synt_2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00980">
        <property type="entry name" value="TRNASYNTHALA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101353">
        <property type="entry name" value="Putative anticodon-binding domain of alanyl-tRNA synthetase (AlaRS)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="ThrRS/AlaRS common domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50860">
        <property type="entry name" value="AA_TRNA_LIGASE_II_ALA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 15" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="8 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 15" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10 15" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 15" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 15" id="KW-0820">tRNA-binding</keyword>
    <keyword evidence="7 15" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alanyl-transfer RNA synthetases family profile" evidence="17">
        <location>
            <begin position="10"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="435"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="604"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="608"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="723"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="727"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00055137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAZ84019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="959" mass="107142" checksum="8B3F51D827AC5DBD" modified="2010-06-15" version="1">MASPLSTVEWPASLVRKTFIDYFKNNGHTFVPSSSVVPLSDPTLLFANAGMNQYKSIFLGTVDPSSDFASLKRAANSQKCIRAGGKHNDLDDVGKDSYHHTFFEMLGNWSFGDYFKKEAVEYSWELLTEIYGLDPDRLYVTYFEGNKERGLEPDLETQELWRAVGVADNHILPGNMKDNFWEMGDQGPCGPCSEVHYDHIGGRNASHLVNMDDPNVIEIWNNVFIQYNREPDHSLRSLPNKHVDTGMGFERLVSVLQDKSSNYDTDVFSPLFQKIQEITKARPYGGKFGAEDVDGIDTAYRVIADHVRTLTFAIADGGVPNNEGRGYVVRRVLRRGARYARRYFQAPIGEFFSKIVPTLVLQLGDVFPEIKKKESDVKEILNEEEESFSRTLDRGERMFDVFSKRATEHNSKVLKGSDVWRLYDTFGFPVDLTRIMAEENGLEINEEEFERAKAESKEASKARKSSGDVELLKLGVHDLGRLEQMAEVPKTDDVYKFGRGNITGHIMAIYCDKSFRSSTANISRYTQLGLILDKTNFYAEQGGQEYDTGKILIDGEAELDVQNVQVYGGYVLHTGYIKYGTLSVGDDVICEYDELRRWPIRNNHTGTHVLNYALREVLGDGIEQKGSLVAAEKLRFDFSHKCGVTDAELEMIEKISTEYIRQNLEVYSEDVPLSIAREIEGVRAVFGETYPDPVRVVSVGVPIEELTRDVKNKQWRKVSIEFCGGTHVQKTDDIKDLIILEENGIAKGIRRIVAVTGNDAGKVQKIASQFEERINALGKLPAGPRKEAEIKSVQADLNSLVISAVKKVQLKDKFGKIHKQLLDDQKAKQKAELKKAVEIVTEHFKQNPDSPYFVSKLPISANSKALSDAIKHVQTKAKDKTVYLFATDDDAEGKVIHGCYVAENVGKQLSAKDLADSISQIVGGKAGGKGATSQGIGNKVDKVGEAVDAAAKALQQLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHX8</accession>
    <name>D5GHX8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00008179001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430322">
        <property type="protein sequence ID" value="CAZ84121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839930.1">
        <property type="nucleotide sequence ID" value="XM_002839884.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHX8"/>
    <dbReference type="STRING" id="656061.D5GHX8"/>
    <dbReference type="GeneID" id="9184214"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008179001"/>
    <dbReference type="eggNOG" id="KOG0737">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004223_1_0_1"/>
    <dbReference type="InParanoid" id="D5GHX8"/>
    <dbReference type="OMA" id="CRFIAHR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056027">
        <property type="entry name" value="DUF7608"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051701">
        <property type="entry name" value="Mito_OM_Translocase_MSP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45644:SF56">
        <property type="entry name" value="AAA ATPASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G12920)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45644">
        <property type="entry name" value="AAA ATPASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G12920)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24581">
        <property type="entry name" value="DUF7608"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="685"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="95"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="925"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="581"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="111"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ84121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="958" mass="105449" checksum="14C2A6ADF245031E" modified="2010-06-15" version="1">MHTITRRVVSSLVPLSRRALLHKRPRPRTIRHFHLSRNALNNSKTSPISVTELRTEKIPASAPVSQDPTSDDIQATLEGESIYATEPVAERVVATETGGDGGNGDSDGRPRARGRPAGVKRRPVVKLRDDAVLPLKVPEWFVDQRVRLLEDEAALGPKAVITSSSSDYVPSTLPGASLDYPPPDTTPEGLNKQILTRYLLEENVWREVVATLRAGLASPKRLSSHSLAALKSHCILHSPIGGSVYQLDAIVNKVAQDVGADVIRIDPLDLEELLGESVGDSRLGTSSPISSFSIRSLGYDIFAPKEKTFVPAQRAPTNWTQLTQLLGFGQVPVFASPLFSITAPPDPEKVSEQSIERANEQKIWSILESVISANRIKRTKGQPTSDTQSSGVPPNQKTIIHVRDFRELERNNSGQAILRIFYEVVLKRRNNGENIVVIGTSSAEEGVENYLTKSGVLTAQLQSGSSFERTIIVPAHPLNPRYRDDKMTRCREVNIRHLGDLLRRKSGENGQSVTIEPMPIGSEAEKEFGITVPSAGANIWNFEHVHRLCTLILGELNGRTSATRHDIKQAIEILDRSDSAKIQWAMTADRAEQELAEMMNEIEQDEAEKAAALEGKPLRKRPKIPKNCTAHEKKLLGGVIDPAEIHTVFSSVRAPEETIEALKTLTSLSLIRPEAFKYGVLATDRIPGVLLYGPPGTGKTLLARAVAKESGATVLEVSGSEVFDMYVGEGEKNVKAIFSLAKKLSPCVVFIDEADAIFGSRHSHSTRTTHREIINQFLKEWADMQSNAFIMVATNRPFDLDDAVLRRLPRRILVDLPTVEDRQEILKIHLAAEILAPSVSLQSLAEQTTLFSGSDLKNLCVSAALACVREENAAATKAKAEKQSFTFPEKRTLDDRHFQTALQEITASISDDMSSLTAIRKFDEKYGEKSGKRKRKPHWGFGTGDPTESKGTGRVRVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>D5GI49</accession>
    <name>D5GI49_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00008270001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430323">
        <property type="protein sequence ID" value="CAZ84192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840001.1">
        <property type="nucleotide sequence ID" value="XM_002839955.1"/>
    </dbReference>
    <dbReference type="GeneID" id="9181910"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008270001"/>
    <dbReference type="eggNOG" id="ENOG502S40N">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_244029_0_0_1"/>
    <dbReference type="InParanoid" id="D5GI49"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="32"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="149"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="352"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="605"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="663"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="40"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="149"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="355"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ84192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1428" mass="156675" checksum="A7EEDCD753273D20" modified="2010-06-15" version="1">MSAAIPSSALATTKDLKGFKEFKDLKDLKDFKDYSIPPLPRDRDGGQGDWESRSRDKGRDDGPKFSLQELETITSQLSGLTSIANSLQREMANLSRRSKDNATDLISLKEATKSRDEDIRQSLRDLVHNLHSLESDRLLTNPSLPLITSGASTSSRASSYASDNFATPPGARGDKSGLEMATLERVLREMSTKDEQDRVIDLLRDIQDTLNKPQLDRLENEEKILSLLEEFKERDYSKGKELVIPGRERDSDMDEKIVSMLQIIRETLYQREERESDVRIISLLEELKQKEEEGGNAQVISLLEEIRDKEGGSRIDAVLSSLEELKDKGGDERVLSLLEEIKARMDGVKEDLANSIGSSTTKRSPSSQDTTGAESTRDSVESNEILNVLKHIKQGVDSGEETARDVQKLIDEWKERWAGHDKEAHEALKDLMKEIEILVTEQRQAISRLHSTPGLQSTALTMTNPPLPPDLDNEAAITALANIASTTTRTDITLSTINALIKVFHKDMNSANTHTHETVLNISRFLQELGNTVSNTHSQTGETRKLLEVVRSGVSSGNDRLAEFEGATLRKIEELMFLHKHLQKTMLTNGDEMIWKQDVGVRAVVEEFQSEVEELLEKNMEAVKESAEKAVEAINNSNPVSLIEELKSELGAMAQRSLAAFAAADSKEALADLKSELQRATEQSVEAINTLNPVTSIETFRAEVKDLLERILSTSESSSKGLAKLKESAPTQAAIEALKCTLGELIERSFNTSNDQTSAAITDMKSVITDKAGGTEKIQTSIDQLKAEVTELMEKSVTALAPPTSYPEAESIKARIESLALEITKTMDNTIALFSDSSVETDEKLKKAVDELKAELGEVSQSISEAVSSNSTSSLEGVVKEALDEMKEVVGQIEKNTEVERSMVSNPEVSEKMTSAIEDLKKEVHEMVEKSINMAVGTPQTGESTYKIQEAIEGLRADIGSAMEKSLVAASDSGNLKELLEDLRKEFAGMVEKSASMIVPQYGSDEATKELLAEIKNDIASILAKSPASEQSSSDVREAIEGLRREVSELMDKTMSMVAPRPTDTEGLPPRSAERIEGVLEGLKSEIGEMVQHSLAAAIANSTFDSEESVKEAIEELKRDVRDTLQLSMMPLPAENTEAAERIRTVLQGFKTELGELFETRIAVPNVTVMEAIDGLRVQIGALAESSKGGVVTEIVEELKMQTEGLEHKGSDGSVVESIEELRKGVDVLAEKLDASSSTEITSAMEELKREVETLLGKSGDALEALKLQVEGLDIKETIEALKAQFEGLSGISGVPVASGLSATSGEEMKESMESLKRDIEEVMEKSMALGKSPFIKEYLPKPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRHVSCGIAGLRMDKYSEGRDVGPRGEECSSCQGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIB1</accession>
    <name>D5GIB1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008394001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430325">
        <property type="protein sequence ID" value="CAZ84254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840063.1">
        <property type="nucleotide sequence ID" value="XM_002840017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIB1"/>
    <dbReference type="GeneID" id="9184613"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008394001"/>
    <dbReference type="eggNOG" id="ENOG502RYM3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031559_1_1_1"/>
    <dbReference type="InParanoid" id="D5GIB1"/>
    <dbReference type="OMA" id="HIWDYAP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021838">
        <property type="entry name" value="DUF3431"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37490:SF3">
        <property type="entry name" value="DUF3431 DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37490">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11913">
        <property type="entry name" value="DUF3431"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="429" mass="49554" checksum="41B526782C64DBF2" modified="2010-06-15" version="1">MPVTILASTVSQVRAIIITCVVTFFVLYTLSDIYHSTPKPGSSSIESHPRTNNLAADRPVEQAGPKPEDEPEDASEKIPIEEEVIPHGVSVFMPDNFSKHLVLPRMRSEDVTWLTYLPEDLNIITKTYLIDPDEGDTLPSTLTVPINKGHEAQAYLTYIIEHYDNLPDIAIFTHAHSTAWHNNDLQVSSTPVMLLELNYQRVAEHGYMNLRCHWKPGCPGWIRLNTTEYDDFKKEEFVMHESFMELFPEEVMRDGIPDILGTPCCAQFAVSRSRIMQHAKERYVSWRKWITDTPLSDEVSGRVMEYVWHFIFASPSLTRNADRAVYCPAEHVCYCDGYGYCFGDDAKYKRWVEKRDRAREMAERLEAAEEGVGREEGEDEAWRQEGIKQWKRELKKMEWECEVEMQYARRRGSDAKLRRGEVGELEFSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>D5GID0</accession>
    <name>D5GID0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00008430001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the flavin monoamine oxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430326">
        <property type="protein sequence ID" value="CAZ84273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840082.1">
        <property type="nucleotide sequence ID" value="XM_002840036.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GID0"/>
    <dbReference type="FunCoup" id="D5GID0">
        <property type="interactions" value="339"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GID0"/>
    <dbReference type="GeneID" id="9184631"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008430001"/>
    <dbReference type="eggNOG" id="KOG0029">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004498_1_2_1"/>
    <dbReference type="InParanoid" id="D5GID0"/>
    <dbReference type="OMA" id="KTQWHVC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000064">
        <property type="entry name" value="Lysine-specific histone demethylase 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050281">
        <property type="entry name" value="Flavin_monoamine_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007526">
        <property type="entry name" value="SWIRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742">
        <property type="entry name" value="FLAVIN MONOAMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742:SF386">
        <property type="entry name" value="LYSINE-SPECIFIC HISTONE DEMETHYLASE 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04433">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50934">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SWIRM" evidence="7">
        <location>
            <begin position="52"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="HMG box" evidence="6">
        <location>
            <begin position="706"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="3">
        <location>
            <begin position="706"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="664"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ84273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="846" mass="93534" checksum="ADBEF2AF02A537E0" modified="2010-06-15" version="1">MDLDSAPASAKPSSTPTVRTVPDRTLIKGRADFRPKTAIPQDLTAEEYARQCVQAAIASRLPPHALHPNEYSLLKDHINHLQVTTYIHIRNGILRLWQRNPLVSVTREEAAGCAKDYRFFDVAEVAYEVLVRGGYINFGCVEVPSTIPANLGNAKRGKTIVVIGAGMSGLGCARQLEGLFTQFGDRLPAGEGMPKVIVLEARGRLGGRIYSHPLKSQAGVNLPEGKRATADLGAQVITGFDNGNPLGVLIRGQLALHYHSLKDNSSLYDSDGTLAPKDRDMLVERLYNDILDRETIILEPHGSDSRHPTLGKTMDSVLRQYQDIIDIAPRDLRLINWHYANLEYANAANVDLLSLGHWDQDDGNDFSGAHAMLLGGYTQLPRGLWLSPRKLDLRTRHVVKKISYNSSKGVEGGARIQCENGETLSADKVVITVPLGVLKAETVTFEPPLPEWKSGAIERLGYGLLNKVILVYDVPFWDVENDMVGLLRDPLGDPTIQESYESNRGRFYMFWNCTKASGKPTLVALMAGDAATQTELESDDTLINEATTALSKMYSDKPVPLPTETIVTRWQKDPYSRGSYSFVGSEATADDYDIMAKPVGNSLYFAGEASCRAYPATVHGAYISGLQAASEIAGSILGPIQVPSPLIPPKPKFQGSYSSLSGSKRKAEDSVIEKARELKSARLEVYEEQLKQALVKALGERPTKPGRSGANPFLLYQKDHWFICKAKCDEARRKATNNPEAKATRNEVRAALGQMWRDAPEDEKRPYLNETENNKRSNAASVTEFKRKLAEWDKAAVKFRKEWKEANPSVPSEDEVLLARQAELEIAEIKRSRKLNGFIDSDDEGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIN4</accession>
    <name>D5GIN4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008583001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430327">
        <property type="protein sequence ID" value="CAZ84377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840186.1">
        <property type="nucleotide sequence ID" value="XM_002840140.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIN4"/>
    <dbReference type="STRING" id="656061.D5GIN4"/>
    <dbReference type="GeneID" id="9183311"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008583001"/>
    <dbReference type="eggNOG" id="ENOG502SDD8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059602_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIN4"/>
    <dbReference type="OMA" id="GTFSRCF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="196"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="196"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="217" mass="24374" checksum="6DA2ABD8F87180B1" modified="2010-06-15" version="1">MGWFWGSSSSKDADGSSDPLSHLDPALRDFLLKESPLKPPSPPPPPPPSSKPTPTTPETTETPTRDPRTINSSYGDRYADIWAQYTPQGLTQASKSSQEQLADILQAYKYRKSLIARAALENCVGEQSALHECYRHGSFAQKARGCGGEKGRLQDCYTTQTKFLQAMGYMSDFARSAEVDERIQMHADKLYQEQVRQDELAEEEKRAERAARERRKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIQ2</accession>
    <name>D5GIQ2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">2-oxoglutarate reductase</fullName>
            <ecNumber evidence="4">1.1.1.399</ecNumber>
            <ecNumber evidence="5">1.1.1.95</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00008606001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible oxidation of 3-phospho-D-glycerate to 3-phosphonooxypyruvate, the first step of the phosphorylated L-serine biosynthesis pathway. Also catalyzes the reversible oxidation of 2-hydroxyglutarate to 2-oxoglutarate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(2R)-3-phosphoglycerate + NAD(+) = 3-phosphooxypyruvate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12641"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18110"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="EC" id="1.1.1.95"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(R)-2-hydroxyglutarate + NAD(+) = 2-oxoglutarate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:49612"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15801"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.399"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.399" evidence="4"/>
    <dbReference type="EC" id="1.1.1.95" evidence="5"/>
    <dbReference type="EMBL" id="FN430328">
        <property type="protein sequence ID" value="CAZ84395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840204.1">
        <property type="nucleotide sequence ID" value="XM_002840158.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIQ2"/>
    <dbReference type="STRING" id="656061.D5GIQ2"/>
    <dbReference type="GeneID" id="9183329"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008606001"/>
    <dbReference type="eggNOG" id="KOG0068">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019796_9_2_1"/>
    <dbReference type="InParanoid" id="D5GIQ2"/>
    <dbReference type="OMA" id="FIICLAR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004617">
        <property type="term" value="F:phosphoglycerate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.260:FF:000036">
        <property type="entry name" value="D-3-phosphoglycerate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050857">
        <property type="entry name" value="D-2-hydroxyacid_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42789">
        <property type="entry name" value="D-ISOMER SPECIFIC 2-HYDROXYACID DEHYDROGENASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_6G10090)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42789:SF1">
        <property type="entry name" value="D-ISOMER SPECIFIC 2-HYDROXYACID DEHYDROGENASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_6G10090)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0718">Serine biosynthesis</keyword>
    <feature type="domain" description="ACT" evidence="13">
        <location>
            <begin position="169"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ84395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="239" mass="26208" checksum="F68F8549B37E5196" modified="2010-06-15" version="1">MHNGTWNKISTKCWEIRGKTLGKDRWAMGMKVLYFDDMIGAEQLAQMKQGSYLINASRGSVVDIPALVESMRSGHTAGAALDVYPTEPAANGDYFNDTLNSWGNDLRSLKNLILTPHIGGSTEEAQSAIGIEVGEALVRYINSGSTIGCVNMPEVTLRSLTLAEENHVRVIFIHSNRPGVLRQVNSILGDHNVDKQMSDSRGDVAYLMADISDVNAGDIRSLYESLEALSSKIMTRVLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIT8</accession>
    <name>D5GIT8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Dolichol-phosphate mannosyltransferase subunit 1</fullName>
            <ecNumber evidence="13">2.4.1.83</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">GSTUM_00008671001</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Transfers mannose from GDP-mannose to dolichol monophosphate to form dolichol phosphate mannose (Dol-P-Man) which is the mannosyl donor in pathways leading to N-glycosylation, glycosyl phosphatidylinositol membrane anchoring, and O-mannosylation of proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a di-trans,poly-cis-dolichyl phosphate + GDP-alpha-D-mannose = a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + GDP</text>
            <dbReference type="Rhea" id="RHEA:21184"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5 13">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Component of the dolichol-phosphate mannose (DPM) synthase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 13">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 13">Belongs to the glycosyltransferase 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.83" evidence="13"/>
    <dbReference type="EMBL" id="FN430328">
        <property type="protein sequence ID" value="CAZ84431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840240.1">
        <property type="nucleotide sequence ID" value="XM_002840194.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIT8"/>
    <dbReference type="FunCoup" id="D5GIT8">
        <property type="interactions" value="828"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GIT8"/>
    <dbReference type="GeneID" id="9185649"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008671001"/>
    <dbReference type="eggNOG" id="KOG2978">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033536_13_3_1"/>
    <dbReference type="InParanoid" id="D5GIT8"/>
    <dbReference type="OMA" id="KCFRREV"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004582">
        <property type="term" value="F:dolichyl-phosphate beta-D-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006066">
        <property type="term" value="P:alcohol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006720">
        <property type="term" value="P:isoprenoid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035269">
        <property type="term" value="P:protein O-linked glycosylation via mannose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06442">
        <property type="entry name" value="DPM1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000036">
        <property type="entry name" value="Dolichol-phosphate mannosyltransferase subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039528">
        <property type="entry name" value="DPM1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43398">
        <property type="entry name" value="DOLICHOL-PHOSPHATE MANNOSYLTRANSFERASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43398:SF1">
        <property type="entry name" value="DOLICHOL-PHOSPHATE MANNOSYLTRANSFERASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 13" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0464">Manganese</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="14">
        <location>
            <begin position="15"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00006739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU365083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAZ84431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="247" mass="27733" checksum="7D355BCE2CCC153F" modified="2010-06-15" version="1">MAPRQSTKSRKDKYTIILPTYNERKNLPVVTWLLEKTFTEHKLDWELVIVDDASPDGTQEVAKQLISVYGADRIVLKPRAGKLGLGTAYVHGLQFATGNFVIIMDADFSHHPKFIADFIALQKTKDYDIVTGTRYAGNGGVYGWDFKRKLVSRGANLLASVVLRPNVSDLTGSFRLYKRKVLETVISQTESKGYTFQMEMMVRARGLGYSVAEVPISFVDRIYGDSKLGGDEIVEYAKGVLNLWLKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIV6</accession>
    <name>D5GIV6_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Copper transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00008704001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the copper transporter (Ctr) (TC 1.A.56) family. SLC31A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840258.1">
        <property type="nucleotide sequence ID" value="XM_002840212.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIV6"/>
    <dbReference type="FunCoup" id="D5GIV6">
        <property type="interactions" value="497"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GIV6"/>
    <dbReference type="GeneID" id="9185667"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008704001"/>
    <dbReference type="eggNOG" id="KOG3386">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079690_4_0_1"/>
    <dbReference type="InParanoid" id="D5GIV6"/>
    <dbReference type="OMA" id="DITEYCH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005375">
        <property type="term" value="F:copper ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007274">
        <property type="entry name" value="Cop_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483:SF115">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 31 COPPER TRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04145">
        <property type="entry name" value="Ctr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0186">Copper</keyword>
    <keyword evidence="4" id="KW-0187">Copper transport</keyword>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="125"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="148"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ84449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="175" mass="19547" checksum="5C272676570E53FA" modified="2010-06-15" version="1">MDHSTHDHHAHMMHASANHDMHASPEAHRCQMSMLFTWSTKDLCLVFRSWHVHNTTTLILSLFAVIALSAGYEFVRDLARRYEARVDGSLSISEETSSLLPGRAGIAAGTDEVERRRRSGRFGKAVFYGVQVFYSFFIMLLFMTYNGWVMISVAIGSAIGYTLWGSGAAKSVACH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJ10</accession>
    <name>D5GJ10_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Restriction of telomere capping protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00008770001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in a process influencing telomere capping.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RTC4 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840312.1">
        <property type="nucleotide sequence ID" value="XM_002840266.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJ10"/>
    <dbReference type="GeneID" id="9181844"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008770001"/>
    <dbReference type="eggNOG" id="ENOG502SEU0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_395976_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJ10"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039024">
        <property type="entry name" value="RTC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028094">
        <property type="entry name" value="RTC4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41391">
        <property type="entry name" value="RESTRICTION OF TELOMERE CAPPING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41391:SF1">
        <property type="entry name" value="RESTRICTION OF TELOMERE CAPPING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14474">
        <property type="entry name" value="RTC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01312">
        <property type="entry name" value="RTC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Restriction of telomere capping protein 4 C-terminal" evidence="9">
        <location>
            <begin position="555"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="99"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="346"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="446"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="99"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="131"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="216"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="254"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="367"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ84503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="696" mass="76279" checksum="93A3B5312E6E6717" modified="2010-06-15" version="1">MPLTRKSTGIKPSTTSTYSAGGEKSLKRPRITKPIFMEVGPKFGGGRGKRQTYMIPGDEDGEGGTPAGIRANSQFSSAYAIRSPPHSPLHVTSVNTGTVISTESPQSDDPLSNLNAPTIAETRSARRTLTRKSVSVLSTNTNENGSVAPRFFVPYGPGDEHKKKRRLDYTQTADSLDYHDSAQGMLAERLSRSRSRSLGRDLEPPNNSVVGEEKTSISLSGDTISSSSSPLSPLPSSSRSSPILIDGDSPQKADPSNDNGSNTPSLVNTDPTNGDDSLCGPTGGKAKAPPVPSWKTGEEKRGGKRPVTFKKATYGTNGKGGYSTIPYAKELMKSHPFARALSRAEKRQQELQEESETVTTPTAAPRVGKRKDKTSKRSSRLLLATHKRDGDLSLEGDEEAVAESSFINPREKFGKKSCFTSALRFAIDLGIGFGVMREVSLKRLSDDDLDGDDDLSSKSPGPAIEGRNLAQSPPPGAEKGVCQGCNEPLPTGFLDTWKNGNFSEGHIPMLEWMDICTEHKAVSLREEWMERGYPEIDWKGMKKRIETHYKPIKAIVHGETESPFMAIFKQQEKDVRGNPILLIQKDHRLQYPGYYGPRGANIMLHWLLRRFGNEITTRNNETSNLAHSSAAAFVQAVLVPEMAVRLIMEDMKITEKRAREVLEESRSIGEILNLQDYQEDIYGTGGNWVDVNNIKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJD8</accession>
    <name>D5GJD8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00008953001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PA-phosphatase related phosphoesterase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430330">
        <property type="protein sequence ID" value="CAZ84631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840440.1">
        <property type="nucleotide sequence ID" value="XM_002840394.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJD8"/>
    <dbReference type="STRING" id="656061.D5GJD8"/>
    <dbReference type="GeneID" id="9182632"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008953001"/>
    <dbReference type="eggNOG" id="KOG3030">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_950588_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJD8"/>
    <dbReference type="OMA" id="GHSIAAW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008195">
        <property type="term" value="F:phosphatidate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046839">
        <property type="term" value="P:phospholipid dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043216">
        <property type="entry name" value="PAP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165">
        <property type="entry name" value="LIPID PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165:SF84">
        <property type="entry name" value="PHOSPHATIDIC ACID PHOSPHATASE BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="45"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="128"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="158"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="190"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="7">
        <location>
            <begin position="50"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ84631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="293" mass="32106" checksum="71446758D870D99D" modified="2010-06-15" version="1">MIAVFIPIISIILINTLGIATHGPHGKRLRTAGMRGGALLGRGSFWNVNNGCMGVIYSVLTGATLQIIIKLVIGGLRPHFLTVCDPQIDGVQGMGYGGLYFDTSVCRDHTDPKRRDEIANAMQSFPSGHSIAAWAGLLYLSLYFNAHLKIFSNYHPSYWKLLLFITPMLAATLIVGSLSLDMSHNWYDTVVGSVMGVVMAILSYRMCFAAVWDFRFNHIPLARDPRIPGFGYSIEELEDWSGGCATRRGGWGVARRYTTFNHRWGAPGDCAFQSAGVSSGLGGVDSMDGQRRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJQ1</accession>
    <name>D5GJQ1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00009123001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430332">
        <property type="protein sequence ID" value="CAZ84744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="tml:GSTUM_00009123001"/>
    <dbReference type="HOGENOM" id="CLU_3435095_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJQ1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ84744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="14" mass="1509" checksum="841BFFF44662FCA5" modified="2010-06-15" version="1">MPTPCVSSALRSLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>D5GL86</accession>
    <name>D5GL86_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00010058001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430346">
        <property type="protein sequence ID" value="CAZ85279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841088.1">
        <property type="nucleotide sequence ID" value="XM_002841042.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GL86"/>
    <dbReference type="STRING" id="656061.D5GL86"/>
    <dbReference type="GeneID" id="9182073"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010058001"/>
    <dbReference type="eggNOG" id="ENOG502SMFK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010595_0_2_1"/>
    <dbReference type="InParanoid" id="D5GL86"/>
    <dbReference type="OMA" id="WAPRNCE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591:SF10">
        <property type="entry name" value="ABC TRANSMEMBRANE TYPE-1 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13489">
        <property type="entry name" value="Methyltransf_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="345" mass="38764" checksum="478276E213E2273D" modified="2010-06-15" version="1">MSDSHQSIAPSPPSQFPALDIETNPHDYSDIDSGFGASIARTENSYSMIAGSGIHPYVYENGRRYQVYREGVYMLPNDAQEIERLDHFHRIFLALLDGKLFIAPLGQDGHRVRNVLDIGTGTGTWAIEMAEEFPDAKVMGNDLSKIQPSWAPRNCEFEIDDVESDWTYPENHFDFIHLRSLSGCFQDWDMVIRRCYDHTAPGGYIEFQDYDGNLKSGLGTMPDNAVSRYWGVVSEAASSTGRTFLVASTIEERIKAAGFVDVTVKKEMWPLGPWPKDERLKEIGRLGRTGMRSAVHAFASALLTRVLGWDEMQVQKLCQEATQEVNDGSAQLYVTAYFVCGRKPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLA9</accession>
    <name>D5GLA9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00010108001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat G protein beta family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430348">
        <property type="protein sequence ID" value="CAZ85302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841111.1">
        <property type="nucleotide sequence ID" value="XM_002841065.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLA9"/>
    <dbReference type="FunCoup" id="D5GLA9">
        <property type="interactions" value="423"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GLA9"/>
    <dbReference type="GeneID" id="9182096"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010108001"/>
    <dbReference type="eggNOG" id="KOG0286">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_57_34_1"/>
    <dbReference type="InParanoid" id="D5GLA9"/>
    <dbReference type="OMA" id="PLDSQWV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000020">
        <property type="entry name" value="Guanine nucleotide-binding protein beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016346">
        <property type="entry name" value="G-protein_beta_1-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001632">
        <property type="entry name" value="WD40_G-protein_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19850">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN BETA G PROTEIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25391">
        <property type="entry name" value="WD40_Gbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002394">
        <property type="entry name" value="GN-bd_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00319">
        <property type="entry name" value="GPROTEINB"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="56"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="144"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="185"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="228"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="279"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="314"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ85302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="347" mass="38302" checksum="F53714F7A63D1C51" modified="2010-06-15" version="1">MSDIQDRIAAARREAEGLKAKIKIKKEELADADLRQVAKETLEPLPRTQMKVRKTLKGHLAKIYAMHWSTDRRHLVSASQDGKLIIWDAYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNICSVYNLSARDGPTRVARELSGHSGYLSCCRFINDRRVLTSSGDMTCMLWDIDTGAKITEFSDHLGDVMSLSINPTNQNIFVSGACDAFAKLWDIRTGKAVQTFAGHESDINAVQFFPDGNAFGTGSDDATCRLFDIRADRELNTYSSDQILCGITSVAFSVSGRLLFAGYDDFECKVWDVLRGERVGTLTGHDNRVSCLGVSNDGISLCTGSWDSTLKIWAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLE9</accession>
    <name>D5GLE9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00010166001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bS6 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430348">
        <property type="protein sequence ID" value="CAZ85342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841151.1">
        <property type="nucleotide sequence ID" value="XM_002841105.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLE9"/>
    <dbReference type="FunCoup" id="D5GLE9">
        <property type="interactions" value="208"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GLE9"/>
    <dbReference type="GeneID" id="9186086"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010166001"/>
    <dbReference type="eggNOG" id="KOG4708">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126331_1_0_1"/>
    <dbReference type="InParanoid" id="D5GLE9"/>
    <dbReference type="OMA" id="RGVQYWG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15465">
        <property type="entry name" value="bS6_mito"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000529">
        <property type="entry name" value="Ribosomal_bS6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035980">
        <property type="entry name" value="Ribosomal_bS6_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014717">
        <property type="entry name" value="Transl_elong_EF1B/ribsomal_bS6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21011">
        <property type="entry name" value="MITOCHONDRIAL 28S RIBOSOMAL PROTEIN S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21011:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN BS6M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01250">
        <property type="entry name" value="Ribosomal_S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54995">
        <property type="entry name" value="Ribosomal protein S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="116" mass="13339" checksum="5BD65097885A8F0A" modified="2010-06-15" version="1">MLYELVAVVRTSSLNEIKEIAKSAGMTVLQNGGVVRGYTYWEKLMLPTRRRAHLVYHTHGQYFIMRFDSNSPTQHLIQRTLRLDPRMISFSVVKMGTKLKDVAKVKGKTWDLDLGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLX7</accession>
    <name>D5GLX7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00010465001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430351">
        <property type="protein sequence ID" value="CAZ85544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841353.1">
        <property type="nucleotide sequence ID" value="XM_002841307.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLX7"/>
    <dbReference type="STRING" id="656061.D5GLX7"/>
    <dbReference type="GeneID" id="9187324"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010465001"/>
    <dbReference type="eggNOG" id="ENOG502QWPZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043034_3_1_1"/>
    <dbReference type="InParanoid" id="D5GLX7"/>
    <dbReference type="OMA" id="ASYYCTY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011089">
        <property type="entry name" value="GmrSD_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24094:SF15">
        <property type="entry name" value="AMP-DEPENDENT SYNTHETASE_LIGASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24094">
        <property type="entry name" value="SECRETED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07510">
        <property type="entry name" value="GmrSD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013197910" evidence="1">
        <location>
            <begin position="16"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="GmrSD restriction endonucleases C-terminal" evidence="2">
        <location>
            <begin position="106"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ85544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="205" mass="22095" checksum="275CE619A7898A1B" modified="2010-06-15" version="1">MKLSAIALFISAATATPVNELVKRTPPNIPSAASAISMLASLTVRTSDPSGYDRNLFPHWITISGSCNTRETVLARDGTNVIQASDCSAVSGSWYSPYDGATWTAASDVDIDHVIPLSDAWKSGANTWTTALRQQFANDLTNPQLIAVTDNVNQAKGDKSPDLWKPPLTSYYCTYAKMWVKVKSAWSLSVKSAEKSALASMLNTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5GM34</accession>
    <name>D5GM34_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00010507001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430351">
        <property type="protein sequence ID" value="CAZ85577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841386.1">
        <property type="nucleotide sequence ID" value="XM_002841340.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GM34"/>
    <dbReference type="GeneID" id="9187361"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010507001"/>
    <dbReference type="HOGENOM" id="CLU_2656251_0_0_1"/>
    <dbReference type="InParanoid" id="D5GM34"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ85577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="76" mass="8800" checksum="A53621A3073EC929" modified="2010-06-15" version="1">MAVYEKQRRRFPIACGRGSLRNEKRLFVYCSPALHHTVLGYRWGRVIGCPWVMCALFPWGDMGFGAVPWGYFCITL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GM86</accession>
    <name>D5GM86_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00010612001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430353">
        <property type="protein sequence ID" value="CAZ85640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841449.1">
        <property type="nucleotide sequence ID" value="XM_002841403.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GM86"/>
    <dbReference type="GeneID" id="9187424"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010612001"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_43_5_1"/>
    <dbReference type="InParanoid" id="D5GM86"/>
    <dbReference type="OMA" id="MPLYNRD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015798">
        <property type="term" value="P:myo-inositol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015791">
        <property type="term" value="P:polyol transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000474">
        <property type="entry name" value="Sugar transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050814">
        <property type="entry name" value="Myo-inositol_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020:SF25">
        <property type="entry name" value="SUGAR TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_7G05830)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="181"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="211"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="240"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="278"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="399"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="433"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="463"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="498"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="524"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="113"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ85640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="635" mass="71289" checksum="259F0B7BD67BCF46" modified="2010-06-15" version="1">MADIQKASNVADGEKVAPASISDSIPDRILANPLAHFRPAELEALGRNFVVEKGLDAKYRELFQRAALLAQNPLSFRDEDSEFRTNPRLGAEREALERELTHRWDQPKSLYALVVMCSLGAAVQGWDETAVNGANTFYQKAFGITDNRWLYGFVNSAPYLCCALIGCWLTEPLNHFCGRRGTIFISCVISSVTCLGQGLTQTWQQMLATRFLLGLGIGPKSATIPVYASECTPPTIRGGLVMMWQMWTAFGIMLGLVMGVALFHIGDSTDQAFINWRLMIASPMFFPLIVCALVYFSPESPRWYIKKGNYESAFRSLIRLRHSKIQAARDLIYIDVLLEAEKRISNRRNRLVEVFTVPRNRHALQASLTVMIMQQFCGVNVLAYYSSEVLKGSKTDYNSIRYALLGSMGFGIINFLFAIPAVRTIDTFGRRNLLLFTFPLMAICMLITFFLEDIHNKKDGAPLSLIGMYLFAAVYSPGEGPVPFVYSAECYPLYIRDIGMAIATAVTWFFNWLLTITWPPLHDAIHSAAFGVYAALNVIGFVVILLLVPETRNRTLEELNSVFEVPTRDQAMYGIRQLWWFFGSWIWGKGEQVEKPVLVVGNRSREDVAFPPREGEFGIRAFPPGRDSSSVHGAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMB5</accession>
    <name>D5GMB5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ubiquinone biosynthesis O-methyltransferase, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">3-demethylubiquinol 3-O-methyltransferase</fullName>
            <ecNumber evidence="5">2.1.1.64</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">3-demethylubiquinone 3-O-methyltransferase</fullName>
            <ecNumber evidence="5">2.1.1.-</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Polyprenyldihydroxybenzoate methyltransferase</fullName>
            <ecNumber evidence="5">2.1.1.114</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">COQ3</name>
        <name evidence="6" type="ORF">GSTUM_00010637001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">O-methyltransferase required for two non-consecutive steps during ubiquinone biosynthesis. Catalyzes the 2 O-methylation of 3,4-dihydroxy-5-(all-trans-polyprenyl)benzoic acid into 4-hydroxy-3-methoxy-5-(all-trans-polyprenyl)benzoic acid. Also catalyzes the last step of ubiquinone biosynthesis by mediating methylation of 3-demethylubiquinone into ubiquinone. Also able to mediate the methylation of 3-demethylubiquinol into ubiquinol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 3,4-dihydroxy-5-(all-trans-polyprenyl)benzoate + S-adenosyl-L-methionine = a 4-hydroxy-3-methoxy-5-(all-trans-polyprenyl)benzoate + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:44452"/>
            <dbReference type="Rhea" id="RHEA-COMP:10930"/>
            <dbReference type="Rhea" id="RHEA-COMP:10931"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64694"/>
            <dbReference type="ChEBI" id="CHEBI:84443"/>
            <dbReference type="EC" id="2.1.1.114"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 3-demethylubiquinol + S-adenosyl-L-methionine = a ubiquinol + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:44380"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="Rhea" id="RHEA-COMP:10914"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:84422"/>
            <dbReference type="EC" id="2.1.1.64"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 3-demethylubiquinone + S-adenosyl-L-methionine = a ubiquinone + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:81215"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:19654"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:231825"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of a multi-subunit COQ enzyme complex, composed of at least COQ3, COQ4, COQ5, COQ6, COQ7 and COQ9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Mitochondrion inner membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
            <orientation evidence="5">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. UbiG/COQ3 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.64" evidence="5"/>
    <dbReference type="EC" id="2.1.1.-" evidence="5"/>
    <dbReference type="EC" id="2.1.1.114" evidence="5"/>
    <dbReference type="EMBL" id="FN430354">
        <property type="protein sequence ID" value="CAZ85658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841467.1">
        <property type="nucleotide sequence ID" value="XM_002841421.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMB5"/>
    <dbReference type="FunCoup" id="D5GMB5">
        <property type="interactions" value="306"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GMB5"/>
    <dbReference type="GeneID" id="9184309"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010637001"/>
    <dbReference type="eggNOG" id="KOG1270">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042432_3_1_1"/>
    <dbReference type="InParanoid" id="D5GMB5"/>
    <dbReference type="OMA" id="LASRWWD"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031314">
        <property type="term" value="C:extrinsic component of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061542">
        <property type="term" value="F:3-demethylubiquinol 3-O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120537">
        <property type="term" value="F:3-demethylubiquinone 3-O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010420">
        <property type="term" value="F:polyprenyldihydroxybenzoate methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00472">
        <property type="entry name" value="UbiG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010233">
        <property type="entry name" value="UbiG_MeTrfase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01983">
        <property type="entry name" value="UbiG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43464">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43464:SF19">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS O-METHYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13489">
        <property type="entry name" value="Methyltransf_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 5" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="binding site" evidence="5">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ85658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="311" mass="34614" checksum="471134941FD7F7C6" modified="2010-06-15" version="1">MSSRIPPLLRIRSPLIRFSKSLRTNPTRPHSTTSSVDQTEISHFANLASTWWDPHGSSRLLHLMNPIRLSFIQRCILKGSPIPATSSTRTYTEKLDPTVAVQDADNPQKLCFLDIGCGGGILTEALARLPSTESVVAIDPSKEVLSVALEHARQDPMLTGSKLRYINTTIEQLPKELEGQRNQFDVVTLMEVIEHVPRPSSFLRTTMEHVRPGGWLVLSTIARTWASFLTTKFVAEDILGIVPRGTHDWNKYINAEELGEFFKETGGWGDVLVMGCVYVPGFGWREVRGGERVGNYFFGVRRGLGEGVGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMY5</accession>
    <name>D5GMY5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00011024001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATP11 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430361">
        <property type="protein sequence ID" value="CAZ85898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841707.1">
        <property type="nucleotide sequence ID" value="XM_002841661.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMY5"/>
    <dbReference type="FunCoup" id="D5GMY5">
        <property type="interactions" value="289"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GMY5"/>
    <dbReference type="GeneID" id="9183466"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011024001"/>
    <dbReference type="eggNOG" id="KOG3281">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054226_1_0_1"/>
    <dbReference type="InParanoid" id="D5GMY5"/>
    <dbReference type="OMA" id="FLQWGFH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033615">
        <property type="term" value="P:mitochondrial proton-transporting ATP synthase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010591">
        <property type="entry name" value="ATP11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13126:SF0">
        <property type="entry name" value="ATP SYNTHASE MITOCHONDRIAL F1 COMPLEX ASSEMBLY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13126">
        <property type="entry name" value="CHAPERONE ATP11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06644">
        <property type="entry name" value="ATP11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="79"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="95"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="106"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ85898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="322" mass="36064" checksum="1143456930B8B110" modified="2010-06-15" version="1">MASVIRPFITRRVIVPQFKIAPRIQRRNAQVLNVRFLQTPTAPLVLEKYREKLEAKLKSEGLGSLEELREVYRDRIENVRKESAPPRSPIDPLLQDPPPPPPSPQPSSSVETASASSPSSVQTGGARSTDAPPPGVKTLSSFVDVDRLTAHSDPKEIEFIWRARFLDDKNSLCAVIPKDTYASLEESACQYPMFILPLPRDNGAEMHFLQWTFPTPRTSTIIFASLAEYKLRGEFAVPHTTFSHHLELAEDKGIVLAQGAVTPDRGVTANDARWLVMALQRFYGALDSEESGVRRKRLLEMFSKGDDGFKVESLIEEVEKWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMZ5</accession>
    <name>D5GMZ5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00011043001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Endomembrane system</location>
            <topology evidence="5">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430361">
        <property type="protein sequence ID" value="CAZ85908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_002841671.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMZ5"/>
    <dbReference type="FunCoup" id="D5GMZ5">
        <property type="interactions" value="26"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GMZ5"/>
    <dbReference type="GeneID" id="9183476"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011043001"/>
    <dbReference type="eggNOG" id="KOG0724">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037236_1_0_1"/>
    <dbReference type="InParanoid" id="D5GMZ5"/>
    <dbReference type="OMA" id="RYIRHAR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052606">
        <property type="entry name" value="DnaJ_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44653">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44653:SF2">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
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    <keyword evidence="1 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072167" evidence="8">
        <location>
            <begin position="21"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="147"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="9">
        <location>
            <begin position="44"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="207"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="316"/>
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        </location>
    </feature>
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            <begin position="216"/>
            <end position="234"/>
        </location>
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            <begin position="235"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
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            <begin position="327"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="352"/>
            <end position="361"/>
        </location>
    </feature>
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        <location>
            <begin position="362"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="372"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ85908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="383" mass="42521" checksum="3196FA4B79724628" modified="2010-06-15" version="1">MRSPFLVLMLIASLATSAFAWSKEDHEIFRLHDEVQLHEGKDTTFYDFIGLKNGPSASQSDITKAYKKKSVRMHPDKHKPKPPPGVTYTQAQLSELHKQASERFARFRLVVAVLTGPGRERYDHFLKNGFPRWRGTGYYYSRFRPGLGSVLVGLFLFSGAMHYGILWLNSKRQKEFMREYIKNARTAAWGKGGIPGLNDALEAAVPGGEATGGEQKLSRAERRAGKGKAVKESSRSGSATPSTQHMTKRRVQADNGKVLMVDSAGSVYLIEQDGNGKDVHLMLDLDEIEGPKIRNTLLVTLPLWIFDKTIGRLIPGRNATTTGDEAHDSEEDAGEDEEVVTSSAGEEVVVKKMARKKKGVAKKVERSDGLPRRKTAAKRPSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>D5GN81</accession>
    <name>D5GN81_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00011143001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430362">
        <property type="protein sequence ID" value="CAZ85974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002841737.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GN81"/>
    <dbReference type="GeneID" id="9185900"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011143001"/>
    <dbReference type="eggNOG" id="KOG1399">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019225_1_0_1"/>
    <dbReference type="InParanoid" id="D5GN81"/>
    <dbReference type="OMA" id="PLFEYSF"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050346">
        <property type="entry name" value="FMO-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23023">
        <property type="entry name" value="DIMETHYLANILINE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13738">
        <property type="entry name" value="Pyr_redox_3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
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        <location>
            <begin position="19"/>
            <end position="571"/>
        </location>
    </feature>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAZ85974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="571" mass="64629" checksum="28C3E5547F880283" modified="2010-06-15" version="1">MSTKVIVIGAGWFGLVAAKTYLQVEPHVDLTILESDETIGGTWSRSRIYPSLLAQQRYGRYEYSDRNIDIGGGDRNSFVPAFKIHAYMNDYVEEFGIKQRIRFKTVVTRIVRNEDGKRWDVWINDDSELMTCDRLIVATGLTSKPIMPNIPSEKPTSKIFHSRELGTYYDYITSPAVKNVTVYGGGKSAIDAIYSCVTNGKHVNWVVRKGGGGVPPLLTGVANGKVLDDFATSRFSSKLHPSLHTNGWWHWALHSGKNFLGQWLHWKYWGRATNSMQSNVEYTKSENMAKLTPNIVDHGAYWINVGPAVLTQPEIVDWIHCGCQITVHRNTIEKLEGDQVHLDDGSSHRADAVIYATGYDVIQPAFSLEDGANLGLPTPVKQCPPEVSMKWGYLEEVADKEVVERFPRLGKPPPHKKIPVGYTPYRLWRDMVPLPLLSGEKPDRSLVFVGTVKTYSTSIMSEAMALWTVAWMSGRIHPGKSFGEMEHEVAVSNAFARRRYLNLGHKFTYQFYEFLPIVDKLLQDIGVKSQRKPDAISDFFEPYIPEDYRGMVEEFKKAHGISNDEETKKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNE5</accession>
    <name>D5GNE5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00011247001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430363">
        <property type="protein sequence ID" value="CAZ86038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841847.1">
        <property type="nucleotide sequence ID" value="XM_002841801.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNE5"/>
    <dbReference type="GeneID" id="9182238"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011247001"/>
    <dbReference type="HOGENOM" id="CLU_886206_0_0_1"/>
    <dbReference type="InParanoid" id="D5GNE5"/>
    <dbReference type="OMA" id="LAMHTFA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="219"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="158"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="181"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="220"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="241"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="259"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="299"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ86038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="314" mass="33326" checksum="E186E923CB81C0F5" modified="2010-06-15" version="1">MPARAVFIAVSAAIAAGIVVYHERERLLELLDRTSRKVALSLSQIAEDFNPRPRQTEHPMTSRAPPRPRTPSEEQWTEEEKAFIFDEKPTDTEARKRDEDTFGAASGWEGRDGAARDVRRRGSPLAAPADGSVLFDTAPASPAGSSTPTGTAVKVPGSVTCTSFSRRSSSATLSGDRALPNLPPPLPPKPQALAQEAIPTAAAVAAEIVSPLQTPDVLVTTANADSSRTASSSSDHEGRQTENPFGNSQPYWSIHEWAENTTLGSGSRSPSIAGSAAEEIPQPADEILSEFGSEDESVGSWTEVGSSIHSDDDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNL4</accession>
    <name>D5GNL4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00011352001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430366">
        <property type="protein sequence ID" value="CAZ86107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841916.1">
        <property type="nucleotide sequence ID" value="XM_002841870.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNL4"/>
    <dbReference type="GeneID" id="9186183"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011352001"/>
    <dbReference type="HOGENOM" id="CLU_1180947_0_0_1"/>
    <dbReference type="InParanoid" id="D5GNL4"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ86107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="235" mass="26004" checksum="B5CFE3061B0BF426" modified="2010-06-15" version="1">MCTGGRSTSSSFWGYRYHINTTKIRYLPIQLLDDLIQRASCQTSSDTPCNPRALITRCGGAKYDIFSSSTHYGRKGETKKGLYPTLPYYQPFPSLYLHPQFLPLPKLPARVLIFWPPPTKRPTSAPQKKKKKKHDNNSNSPHRPPPPGLNLHPPPPDLRALPTGIHPPNGLNRPIPLNPNPLTPVLAPTPDAAAHACGGSLPPAHAGVPQHGCHPFHRVRDCRAVGLWVPELRIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNP2</accession>
    <name>D5GNP2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Autophagy-related protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00011396001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Preautophagosomal structure membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHEK2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430366">
        <property type="protein sequence ID" value="CAZ86135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841944.1">
        <property type="nucleotide sequence ID" value="XM_002841898.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNP2"/>
    <dbReference type="STRING" id="656061.D5GNP2"/>
    <dbReference type="GeneID" id="9186211"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011396001"/>
    <dbReference type="eggNOG" id="KOG0615">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_281744_0_0_1"/>
    <dbReference type="InParanoid" id="D5GNP2"/>
    <dbReference type="OMA" id="TFCGTML"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0072">Autophagy</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHA" evidence="9">
        <location>
            <begin position="94"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="246"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="domain" description="FHA" evidence="9">
        <location>
            <begin position="895"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="514"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="565"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="631"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="746"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="774"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="529"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="584"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="705"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="722"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="749"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ86135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1044" mass="115146" checksum="ECB6D2DDC9264D9B" modified="2010-06-15" version="1">MSAMDEEATQPATQPLPVHNGVAPSTVHGNYLAGVLCLLHPSSPAAYSIVEKTAKVDPGVVLRIPAAQQGEPEVSGLDIALRLSPGPKDPELGFVFGRNPARCDVVFTESNKSRRISNLHFRIYQNAQGVLMLHDSSTNGTWVDNVKLGGVGADGGKSTDNQRALFAGSVIHLAPGAKLLRFIVRIPTQDGIPPPFPPPKLPLKHTRSPARHLAAELRDPFKVQGKGRHMAKAVKYNDGWDGGERYKFEGIIGKGAFATVRRAIDRRTGDAYAVKSIQKRAFAQSSDRQLGVRKEVEILEKLNHPNIVSYIDCHEDRSHIYIFMELIKGGDLSEYLGKHGALPEPMVQEVSRQVLRGIEYVHSMGISHRDLKPDNILLACDDPIQVKISDFGLAKMVQNEDTFLKTFCGTMLFLAPEVFPGYANGRGAKQRRRYNQAVDMWSFGCVIFMLLTGSAPFQGKNQDDMLKIILSGKFDVAPLEKRLGARSHQAKDFIRRLLQVNPGMRIMETEALRHPWLADGSEEQSSMEVQEEDEDEGLDEEDDEEANAISSFEKVEKQQEKWMMVQGPDARPKFTASPRRLEQEELSDNDEEDSDDEEIETPKREELQDVQLRKAVTGLSFKGEIHIPAVRFDSGSSEGSFASLGDAESNRGSRRMFHPPPGISIQGSSGVFPDHGYSAFSDGNDSLENGRQVEEDFVPSSQTHEPFEYDELRVIKPREGTRSGSISRSVSGGSLSGAEAMVGKLKVHSPSPKDTPSPTDGEFQHQNQMTPLAVNRNMNGDSNNRDNSPTPTAARQITPTPHQRSRRTSPQPDSFESQAAVEFYTPVSLFSSNIPPPPTTPLAIPADENLPPTQPTNFDHHHNRQFAVPTTPWGRLVPMPGSIPHSTISLNRHQISIGRSASCTHTETDIRISKYHIAFQLSPPDYDVDEGSFQPANNMIAWYYVRGTNGIILNGKKKHKGRIGRIYDGDVVYLFKDCKPGDVVEFLGFRCEFGIGQHVRLVEEQSDYEEEVFANAYGTATGSMATTMAVGTSKGTNGSIDGVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNP8</accession>
    <name>D5GNP8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">C2H2 type master regulator of conidiophore development brlA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00011420001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430367">
        <property type="protein sequence ID" value="CAZ86145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841954.1">
        <property type="nucleotide sequence ID" value="XM_002841908.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNP8"/>
    <dbReference type="STRING" id="656061.D5GNP8"/>
    <dbReference type="GeneID" id="9182780"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011420001"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1061621_0_0_1"/>
    <dbReference type="InParanoid" id="D5GNP8"/>
    <dbReference type="OMA" id="NHWTIST"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000072">
        <property type="entry name" value="zinc finger protein 143 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR PROTEIN YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003:SF19">
        <property type="entry name" value="YY2 TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="159"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="186"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="30"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="123"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="207"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ86145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="277" mass="29922" checksum="E85A5DE6C698819D" modified="2010-06-15" version="1">MNNHWTISTSSGRRVSLLCEEEAASDYAYSYASSSSSPTPPLSPDYRHRPYTIRHDSIESASSSSSSLGSSLYSPRTPPILSLPSLEKLLPVPESSYFPNISTPSVTSSPIPSPAMMMIAATSEELSPTSSQAPITSAPPSPKTSSAANPSLKRSSRRYTCQCGKSFTTSGHLARHTRIHTGEKNYVCPETGCGARFSRQDNCMQHYRTHQSGSGSKRAARKRRMSAESAEAVGNKPDMHPQMHSYHTPQPVSYSHYPESFDGGLTALANAAISGYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNR0</accession>
    <name>D5GNR0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00011433001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430367">
        <property type="protein sequence ID" value="CAZ86157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841966.1">
        <property type="nucleotide sequence ID" value="XM_002841920.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNR0"/>
    <dbReference type="FunCoup" id="D5GNR0">
        <property type="interactions" value="620"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GNR0"/>
    <dbReference type="GeneID" id="9182792"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011433001"/>
    <dbReference type="eggNOG" id="KOG3207">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017716_5_1_1"/>
    <dbReference type="InParanoid" id="D5GNR0"/>
    <dbReference type="OMA" id="SEESHMF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd17044">
        <property type="entry name" value="Ubl_TBCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.190">
        <property type="entry name" value="CAP Gly-rich-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036859">
        <property type="entry name" value="CAP-Gly_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000938">
        <property type="entry name" value="CAP-Gly_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044079">
        <property type="entry name" value="Ubl_TBCE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15454">
        <property type="entry name" value="NISCHARIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15454:SF56">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01302">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01052">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74924">
        <property type="entry name" value="Cap-Gly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50245">
        <property type="entry name" value="CAP_GLY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CAP-Gly" evidence="5">
        <location>
            <begin position="23"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ86157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="512" mass="57211" checksum="91EC673594A74FA2" modified="2010-06-15" version="1">MSALHIGQRLSYSGSLCTVRYIGPVTGTDGEWLGVEWDNVSRGKHSGQHGGKRYFEAKLSNSSTFIRTTRAPDPPVTFLEALRNKYTTEKDISFEIGKPISLSATKVFEEVGFDKILEKLGRLRELEVVLLDGMRIVRGDGATEVLDTCPKIEELDVSRNLWEGLEDIAGVCRGLKMLKGLRISGNRFSSLEIGDGDPPFEGVVSLEVNNTLFDWAEVSHILQFFPQIHTLFSASNNTTEIPKSITLPHTLTTINLEQNAFTSLSSIFPLSNLPQLKKLILAHNTISTVHTPESSPVVFPSLEHLDLSFNSLGTYPLLDLLPPLFPCLTSLRISHNPLFSSTPLEEAHMLAVARTPARVITLNYSKITESERANAELYYLNQIAKEMAKVEEGMEDTVLRDHKRWEELCELHGKPIIDRSTEEKKTLATKLIDLEFLSEGKDPLVKKIPKSLSISTLKGLIGRMFGIAPLKVQMEYHPLEEGGDFIPLDDDIKEIGFYIDNREGRIKVAERA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNR2</accession>
    <name>D5GNR2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00011436001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430367">
        <property type="protein sequence ID" value="CAZ86159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841968.1">
        <property type="nucleotide sequence ID" value="XM_002841922.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNR2"/>
    <dbReference type="FunCoup" id="D5GNR2">
        <property type="interactions" value="420"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GNR2"/>
    <dbReference type="GeneID" id="9182794"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011436001"/>
    <dbReference type="eggNOG" id="KOG0069">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019796_1_2_1"/>
    <dbReference type="InParanoid" id="D5GNR2"/>
    <dbReference type="OMA" id="PHIAWAY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030267">
        <property type="term" value="F:glyoxylate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016618">
        <property type="term" value="F:hydroxypyruvate reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12168">
        <property type="entry name" value="Mand_dh_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000282">
        <property type="entry name" value="Glyoxylate reductase protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050223">
        <property type="entry name" value="D-isomer_2-hydroxyacid_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996">
        <property type="entry name" value="2-HYDROXYACID DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996:SF269">
        <property type="entry name" value="HYPOTHETICAL D-ISOMER SPECIFIC 2-HYDROXYACID DEHYDROGENASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00065">
        <property type="entry name" value="D_2_HYDROXYACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00670">
        <property type="entry name" value="D_2_HYDROXYACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase catalytic" evidence="5">
        <location>
            <begin position="24"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="6">
        <location>
            <begin position="123"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ86159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="332" mass="36651" checksum="C4B29CE8868FCC68" modified="2010-06-15" version="1">MSSDKKKILLLGRIDHAHSYWSSLNDVAEVIVPKARNRAEFIEECKSGALDDVFCAYRTFFSVAITGRIDEELVSALPKSLKFLCHKGAGYDQIDPEACTRHDIRVSNTPTAVDDATADVNLFLIIGALRNFNQGMVQLREGHWLKDIPLGHDPEGKVLGILGLGGIGRALKKRTDAFDMKVIYHNRKQLSPDLENGAEYVSFDELLSRSDVISLNLPLNSATRHIISSNEFSKMKDGVVIVNTARGAVMDEEALVQALASGKVRSCGLDVYEEEPKVHPGLLSNPRVMLIPHMGTYSHETLKAMECWTIDNLHSAISTGKLKSPIPEQADM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5GP96</accession>
    <name>D5GP96_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DASH complex subunit DAD2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Outer kinetochore protein DAD2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">GSTUM_00011764001</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
            <location evidence="3">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DASH complex DAD2 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430373">
        <property type="protein sequence ID" value="CAZ86361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842170.1">
        <property type="nucleotide sequence ID" value="XM_002842124.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GP96"/>
    <dbReference type="FunCoup" id="D5GP96">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GP96"/>
    <dbReference type="GeneID" id="9186601"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011764001"/>
    <dbReference type="eggNOG" id="ENOG502SG7I">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_138063_0_0_1"/>
    <dbReference type="InParanoid" id="D5GP96"/>
    <dbReference type="OMA" id="DYEVGVW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042729">
        <property type="term" value="C:DASH complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990023">
        <property type="term" value="C:mitotic spindle midzone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008608">
        <property type="term" value="P:attachment of spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013963">
        <property type="entry name" value="DASH_Dad2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28036">
        <property type="entry name" value="DASH COMPLEX SUBUNIT DAD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28036:SF1">
        <property type="entry name" value="DASH COMPLEX SUBUNIT DAD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08654">
        <property type="entry name" value="DASH_Dad2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="16" id="KW-0137">Centromere</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="11" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="100"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="15"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="116"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00030568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAZ86361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="148" mass="15844" checksum="86AE17944E9746E8" modified="2010-06-15" version="1">MANPYPNRYSQHFRQPSASGSSGMGGSQQSPALSARINEKKQELENLQALRDLSAGLASQMQQLEEKLGTLADGTEAVAAVLSNWHNVLRAIAMASSKIPIPKDPAADDNPFAPTKKTDENEDLGKDGNGVPMPQTLVRIPVLPQPSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPC1</accession>
    <name>D5GPC1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00011795001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430373">
        <property type="protein sequence ID" value="CAZ86386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842195.1">
        <property type="nucleotide sequence ID" value="XM_002842149.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPC1"/>
    <dbReference type="GeneID" id="9186626"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011795001"/>
    <dbReference type="HOGENOM" id="CLU_1705547_0_0_1"/>
    <dbReference type="InParanoid" id="D5GPC1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ86386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="154" mass="16942" checksum="1A3044B65BC37B03" modified="2010-06-15" version="1">MEPLGGNQKSAQVPDLELELPRIPEKAYIPGYLSRDLDLPAVLAPPDPPSSQLALAFGSASSQTSSQDEDSVVGTKVAICFSMEDEGTEYSEYPRNDEMNLKHLKGKINKLQQIRKNSAGKIRFLQKSCEDAMEIAMNAEADLEHVMKLYVSKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPS7</accession>
    <name>D5GPS7_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">endo-polygalacturonase</fullName>
            <ecNumber evidence="3">3.2.1.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">GSTUM_00012011001</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(1,4-alpha-D-galacturonosyl)n+m + H2O = (1,4-alpha-D-galacturonosyl)n + (1,4-alpha-D-galacturonosyl)m.</text>
            <dbReference type="EC" id="3.2.1.15"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the glycosyl hydrolase 28 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.15" evidence="3"/>
    <dbReference type="EMBL" id="FN430378">
        <property type="protein sequence ID" value="CAZ86520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842329.1">
        <property type="nucleotide sequence ID" value="XM_002842283.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPS7"/>
    <dbReference type="FunCoup" id="D5GPS7">
        <property type="interactions" value="114"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GPS7"/>
    <dbReference type="GeneID" id="9182264"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012011001"/>
    <dbReference type="eggNOG" id="ENOG502QTAW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040116_0_0_1"/>
    <dbReference type="InParanoid" id="D5GPS7"/>
    <dbReference type="OMA" id="WTGNSIT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004650">
        <property type="term" value="F:polygalacturonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045490">
        <property type="term" value="P:pectin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000002">
        <property type="entry name" value="Endopolygalacturonase D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000743">
        <property type="entry name" value="Glyco_hydro_28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050434">
        <property type="entry name" value="Glycosyl_hydrlase_28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31884">
        <property type="entry name" value="POLYGALACTURONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31884:SF1">
        <property type="entry name" value="POLYGALACTURONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00295">
        <property type="entry name" value="Glyco_hydro_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00502">
        <property type="entry name" value="POLYGALACTURONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9 13" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072241" description="endo-polygalacturonase" evidence="14">
        <location>
            <begin position="17"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAZ86520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="361" mass="37371" checksum="FB4EA5AC43D8F549" modified="2010-06-15" version="1">MFAFASLSIFASLVTAIPLIARSPSPSCTITSIDDVAACKKSCTDISIGDFTVPAGQTLDLTDLLTGTTVTFTGTLCFGYKEWTGPLVSVSGTDVHIVGAPGSVLDGDGARWWDTKGGNGGKKKPKFFAAHGLKSSSITGLTLKNTPVQAFSISGVEDLLLSSITINNKDGDVNDLGHNTDAFDVGSSDGVTIEGATVYNQDDCLAVNSGRNINFLNCFCSGGHGISIGSVGGRKNNEVRGVTIKDSTVVDSDNGIRIKTVSGTTGSVSDVTYSGITLKNISKRGIVIQQDYENGSPTGVPTDGVPITDLTITNVKGTVTSSATQIYVLCAQDACSDWEWSGVEISGGKYSDCKNAPSGTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPT4</accession>
    <name>D5GPT4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00012023001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430378">
        <property type="protein sequence ID" value="CAZ86527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPT4"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012023001"/>
    <dbReference type="HOGENOM" id="CLU_2361270_0_0_1"/>
    <dbReference type="InParanoid" id="D5GPT4"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="10"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ86527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="96" mass="10430" checksum="F6B66EAC157A2411" modified="2010-06-15" version="1">MPIIGHLSYLFKAGEQSPGGQTRPGPKEETNSRRGYPPPSDLGLKNIADKFSNHVHGSPCAPRAQVPTQCALAFTKEKGKRKEKRSARARAGFADE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5G3W7</accession>
    <name>D5G3W7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00003832001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 15 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835089.1">
        <property type="nucleotide sequence ID" value="XM_002835043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G3W7"/>
    <dbReference type="FunCoup" id="D5G3W7">
        <property type="interactions" value="42"/>
    </dbReference>
    <dbReference type="GeneID" id="9187148"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003832001"/>
    <dbReference type="eggNOG" id="KOG4472">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024327_4_3_1"/>
    <dbReference type="InParanoid" id="D5G3W7"/>
    <dbReference type="OMA" id="WCFFLYQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000051">
        <property type="entry name" value="Alpha-1,2-mannosyltransferase (Ktr4)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002685">
        <property type="entry name" value="Glyco_trans_15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121">
        <property type="entry name" value="ALPHA-1,2 MANNOSYLTRANSFERASE KTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121:SF7">
        <property type="entry name" value="MANNOSYLTRANSFERASE KTR4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01793">
        <property type="entry name" value="Glyco_transf_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018153">
        <property type="entry name" value="Glyco_trans_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR018153-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ79210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="396" mass="46087" checksum="062D4B4AA923B5AA" modified="2010-06-15" version="1">MVTKGILRALITLAALTTLLLAFYNYSSPSVRLGSPAGASGGKFVRPKTGGSLKGIDPLLSPEKGPSGKLWGPGEAERTSATLLSLVRNNELAGMVSAMRDLEKTFNYKFNYPWTFFNDEEFTQEFKDKTQAETKAECRYEVIPKDHWEIPSWINNDLMAESVKLLEEKNVQYSGLKSYHQMCRWNSGKFYDHPALKDIRWYWRVEPKVHFFCDVNYDVFKYMEDNNKTYGFVINIYDSPESIETLWPHSLEFFAENPEYLHPNNAMAWLTDAKNRPEHHRKAGYSTCHFWSNFEIADMNFWRSKKYRDYFDHLDRAGGFFYERWGDAPVHSIALGLMEDKDKIHWFRDIGYQHIPYFNCPSSPKCNGCQPGRFTDGTGLDQEDCRANWFEFAHMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5G3Z1</accession>
    <name>D5G3Z1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Mitochondrial import inner membrane translocase subunit TIM22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00003866001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Essential core component of the TIM22 complex, a complex that mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. In the TIM22 complex, it constitutes the voltage-activated and signal-gated channel. Forms a twin-pore translocase that uses the membrane potential as external driving force in 2 voltage-dependent steps.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the TIM22 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion inner membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Tim17/Tim22/Tim23 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835113.1">
        <property type="nucleotide sequence ID" value="XM_002835067.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G3Z1"/>
    <dbReference type="FunCoup" id="D5G3Z1">
        <property type="interactions" value="627"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G3Z1"/>
    <dbReference type="GeneID" id="9182361"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003866001"/>
    <dbReference type="eggNOG" id="KOG3225">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091077_1_0_1"/>
    <dbReference type="InParanoid" id="D5G3Z1"/>
    <dbReference type="OMA" id="VNPNMAD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042721">
        <property type="term" value="C:TIM22 mitochondrial import inner membrane insertion complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030943">
        <property type="term" value="F:mitochondrion targeting sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039175">
        <property type="entry name" value="TIM22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14110">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14110:SF0">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02466">
        <property type="entry name" value="Tim17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="16"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ79234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="179" mass="18483" checksum="55B4AC341DBC9B41" modified="2010-06-15" version="1">MSFGGFPLGGRGLGGTSSNEPLTPEQQQMQMAKTIQAGMESCPVKTVISGGMGFGLGALFGLFMASMSYDTPMTGLPGAPSASLQNLPLKEQLRRGFKDMGQRSYSTGKNFALVGSIFAGTECCIEGFRAKNDMYNGMSAGCVTGGVLAAKAGPQAAAVGCAGFAAFSAAIDYYLRHMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5G417</accession>
    <name>D5G417_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00003906001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835139.1">
        <property type="nucleotide sequence ID" value="XM_002835093.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G417"/>
    <dbReference type="FunCoup" id="D5G417">
        <property type="interactions" value="228"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G417"/>
    <dbReference type="GeneID" id="9182387"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003906001"/>
    <dbReference type="eggNOG" id="KOG3133">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043063_1_1_1"/>
    <dbReference type="InParanoid" id="D5G417"/>
    <dbReference type="OMA" id="YEPMKEM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033328">
        <property type="term" value="F:peroxisome membrane targeting sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045046">
        <property type="term" value="P:protein import into peroxisome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.900">
        <property type="entry name" value="Pex19, mPTS binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006708">
        <property type="entry name" value="Pex19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038322">
        <property type="entry name" value="Pex19_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12774">
        <property type="entry name" value="PEROXISOMAL BIOGENESIS FACTOR 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12774:SF2">
        <property type="entry name" value="PEROXISOMAL BIOGENESIS FACTOR 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04614">
        <property type="entry name" value="Pex19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="258"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="288" mass="32057" checksum="AA3AED2609169AB5" modified="2010-06-15" version="1">MPPPSKVTVEEDSDDDDLDDLDDLLDEFQSSPQPTGQPPAKTSDTGVSNDNRGPSLESLDDDFARQLQLGMQDLLGEMQDSKELQDQFEHLVKELNDATAVPSPMPSAPSSSRPSDQAAANFQDRIKQTMDRMKSSGAEVDAAVAGSEAEDFLSEMLKQMQGAAGEGESDEDFSKMLLNMMEQLTSKEILYEPMKELNEKYPDWLRSNESKQPAEDLQQYREQYGVVKEIVAKFEEPEYRDESDKDREFIIDRMQKMQAAGAPPTELMGEMGQGLSIPPDVDSNCAVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4J8</accession>
    <name>D5G4J8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00004141001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the GMC oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429987">
        <property type="protein sequence ID" value="CAZ79441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842521.1">
        <property type="nucleotide sequence ID" value="XM_002842475.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4J8"/>
    <dbReference type="STRING" id="656061.D5G4J8"/>
    <dbReference type="GeneID" id="9184064"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004141001"/>
    <dbReference type="eggNOG" id="KOG1238">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002865_5_1_1"/>
    <dbReference type="InParanoid" id="D5G4J8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.560.10">
        <property type="entry name" value="Glucose Oxidase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012132">
        <property type="entry name" value="GMC_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000172">
        <property type="entry name" value="GMC_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007867">
        <property type="entry name" value="GMC_OxRtase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552">
        <property type="entry name" value="GLUCOSE-METHANOL-CHOLINE GMC OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552:SF78">
        <property type="entry name" value="GLUCOSE-METHANOL-CHOLINE OXIDOREDUCTASE N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05199">
        <property type="entry name" value="GMC_oxred_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00732">
        <property type="entry name" value="GMC_oxred_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000137">
        <property type="entry name" value="Alcohol_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00623">
        <property type="entry name" value="GMC_OXRED_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00624">
        <property type="entry name" value="GMC_OXRED_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="4">
        <location>
            <begin position="87"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="5">
        <location>
            <begin position="270"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="538"/>
            <end position="539"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000137-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS00623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS00624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ79441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="607" mass="65814" checksum="17A562877A0855E0" modified="2010-06-15" version="1">MSPPSEVDVIICGGGAAGSVVAGRLATADPSLSILVVEGGKNNHNLLTVVRPALFLTHMTPGSTTARFYKGKASKQLNGREPIIPVANILGGGSSINFLMYTRASRSDYDDWKTEGWTADDLIPLLRKTETYHLENKDPAIHGFEGPLHVSHGGHGSETTDQLLEAAKSMGYKITKDLQDLNESNAFEKWPKWINPETGRRCDAPHGFIHPQIEKGCNLHLLCETKVARVIIENGKAVGVEVVPAEAPADSSVPVTTTIKARKLVVVSSGALGTPQILERSGIGAPEVLNAAGIKQVVDLPGVGTNYQDHNLLLQSFRVAPETTTHDDFIRGSTDVLEQGEKDFANGKGIYTTNFVNAGGKVRPTQEEIEEMGPEFRKLYDEYFKDAPDKPVMFVSLGNAFFGDHTLVPPGKYITIALYLEYPASRGTIHVTSPSPYGEPDLDPGFLTHEADIGPNIWGYKLGREIVRRMPCYRGEVPAFHPKFDPSSAARAREIDIETSEEIQESALTSGKIESLVYTKEDNRAIEEWVRENVATTWHSMGTCAMKPREQGGVVDSKLNVYGVEGLKLADLSICPGNVGANTYSTALLVGEKAAILVGECLGLKNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4J9</accession>
    <name>D5G4J9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00004155001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN429987">
        <property type="protein sequence ID" value="CAZ79442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842522.1">
        <property type="nucleotide sequence ID" value="XM_002842476.1"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G4J9"/>
    <dbReference type="GeneID" id="9184065"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004155001"/>
    <dbReference type="eggNOG" id="KOG4157">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000702_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4J9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051836">
        <property type="entry name" value="Kremen_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002889">
        <property type="entry name" value="WSC_carb-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24269">
        <property type="entry name" value="KREMEN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24269:SF16">
        <property type="entry name" value="PROTEIN SLG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01822">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00321">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51212">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="WSC" evidence="8">
        <location>
            <begin position="870"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="domain" description="WSC" evidence="8">
        <location>
            <begin position="1020"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="domain" description="WSC" evidence="8">
        <location>
            <begin position="1129"/>
            <end position="1222"/>
        </location>
    </feature>
    <feature type="domain" description="WSC" evidence="8">
        <location>
            <begin position="1265"/>
            <end position="1358"/>
        </location>
    </feature>
    <feature type="domain" description="WSC" evidence="8">
        <location>
            <begin position="1374"/>
            <end position="1467"/>
        </location>
    </feature>
    <feature type="domain" description="WSC" evidence="8">
        <location>
            <begin position="1519"/>
            <end position="1612"/>
        </location>
    </feature>
    <feature type="domain" description="WSC" evidence="8">
        <location>
            <begin position="1628"/>
            <end position="1721"/>
        </location>
    </feature>
    <feature type="domain" description="WSC" evidence="8">
        <location>
            <begin position="1795"/>
            <end position="1888"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="973"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1237"/>
            <end position="1260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1487"/>
            <end position="1512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1725"/>
            <end position="1791"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1725"/>
            <end position="1789"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ79442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1909" mass="197746" checksum="FFB723CA902EB3E2" modified="2010-06-15" version="1">MDPNVIKSGTFNQIWKTKGLGNYNGFTEQFYAQPLVYTPADTQYVYVISQQNIAYILDAKTGEIIKHRTIHIPFLVTPDLAGCNDVADCVGSTGTGVIDPDTGTWFFTTKTYADQTKNTPQGLAAGRYYIHAVDALTLEEKPNFPIPLEGMTADNAPWRMFEGGKHHQRPALLQVNNYVYAGFASHCVQWNFTGWVVGWHATEGRIVTKYTMEGGKEATGIGGGIWMSGGGIASDNPGRMFFATGNGYASQLADDPVPGRQPPTALEEAVVNMAIGEDGRVTPTDFFMPWEKRDLDGMDKDLGTSSFVLLEPSVFSTARVQRIGTVAGKTGKLYFLNLDDLGGYQMGENRKDKILQTIALSGPVFASAGTYPHDGGYVYLSPVGQQTVVFKFGINSNGDPVFTQAGKTTETTAGRQGVGHTTVTSMNGESGSGILWISDVDGANLRAYGAVPVNGVLPTLALLNNVGQMKFSRPSFGDGRVYLTSHTGYITAFGSPVNMPLNCSSPYDAGTVPIGNTSTVAITCRAKTPLTINATNLDDGTNFKIFGYTLPNTLATGETFSIRANFTPKTVGPLSTNINIRTINGGTEQFSTNTPVVIRGVAISLNPILTIQPNVLSFGEVITGEDTAGKNLDFTMENDGQSPLVIQDYKISLNTSTGPFLPAQPFPLKAGPFTFVDLPAVNSSIPGDGAVSTTVNFNPKVDGYYKAYLVITTNGGSKFVGAFGTAGGKPKALLEWQLENGTWIAYQKGVPFAFGPVPLGTQAFRKLRLRNLGGTNLTTSVSKPPVSGPLAAVNPLGSIPEGSQVAPNGSIEATLICSPPKSQVNEDPTTLTAVWTMNNNDAAFGKTEVDFTCTGASNQVGPLDPNGQGWYRYLGCFKDANPNRNLEELLYYSANNTNGRCQFDCMNSAGQFAYAATEYEGECWCGTKRGLEKVPDKFCQYLCRGDFREYCGGDGAYMSLFGDRRKVGNFSTISSSTTSTTSTSTSSTSTPISTTTTGNSSITITSTSTSATASSTAPANLTYLGCANEGTTGRALAKDAMADPLMTITLCAAYCTNKGYPLSGLEYSTECYCGNVLENGSTIGGSTACNMPCGGNAATTCGGPGALSVYNNTALVPPKQPATVPSVGNYQSLGCYTEGVNERALAGPGMATADMTVQSCVAYCLAGGFKYAGIEYSTECYCGATIATTAKSAPASECNMLCGGDKYAYCGGPSRLNVYGNLNGTVSSVPPISTTTSTSITSSINGTTTSTTSTSTSSTPTLRPGYTYLGCANEGINGRALTKDATASATMTTIMCQDYCTSKGYSLSGVEYSTECYCGGALENGSIIGGSTACNMPCGGAPSSICGGPGALSVYNNTALVAPKQPAVVPAVGDYKSQGCYTEGVNERALAGPGTASVNMTVQSCVGFCQSGGYKYAGIEYSTECFCGSVIATTAKTAPASECNMLCGGDKLAYCGGPSRLNIYGKVNGTTTTSSSTSIVSTSTSTSTTVASGTTTTSSTTSKSSTSTSSTATPTLRAGYTYLGCANEGTNGRALAKDSTASSTMTTTVCQDYCTGKGYPLSGVEYSTECYCGNVLENGSTIGGSTACTMPCGGASNLTCGGPGALSVYNNTALVAPKQPTVVPAVGDYKSQGCYTEGVNERALAGPGTANANMTVQSCVSYCQGGGYKYAGIEYSTECYCGNAIGTTAKTAPVSECNMLCGGDKFAYCGGPSRLNIYGKVNGTSSSSGSTSIPVTSTSLTSTGSGTTTSASTTSKSSTSTTSGSSTGSSTTLGNSTSTTSMSSSSTATPTLRPGYTYLGCANEGTNGRALSKDATSSSTMTPNVCQDYCTGKGYPLSGVEYSTECYCGNVLENGSTIGGSTSCTMPCGGSPNVICGGPGALSVYNNTAITAPKQPIMIPAAAGYNLTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4K2</accession>
    <name>D5G4K2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00004192001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429988">
        <property type="protein sequence ID" value="CAZ79445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835288.1">
        <property type="nucleotide sequence ID" value="XM_002835242.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4K2"/>
    <dbReference type="GeneID" id="9188383"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004192001"/>
    <dbReference type="eggNOG" id="ENOG502SHQF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028200_0_2_1"/>
    <dbReference type="InParanoid" id="D5G4K2"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF114">
        <property type="entry name" value="MEMBRANE PROTEIN PTH11-LIKE, PUTATIVE (AFU_ORTHOLOGUE AFUA_7G06620)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="17"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="51"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="95"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="128"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="170"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="208"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="245"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="8">
        <location>
            <begin position="35"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="321"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="326"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ79445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="383" mass="41699" checksum="9178B24A34CCC279" modified="2010-06-15" version="1">MVDEPNSMNYPEDRREVVLGVNAACLGCTILFVGLRSYARFSVKGLGTDDVLIFLATLCSIGLFVTCLFAMKYGMGRHVGTISSEELVVYTKMTWIGGLVHTASTAFVKASLLALYLRLSPSRAYRSLSYISLVLVVGHAVSGLTVTTFQCTPVPYLWDKTIKGRCIRTGIFHFVNAGVNVLTNVLICALPMPMLWGVQLPKRQKLGLCVVFSLGALACTASIIRLLCLRGLLKSHDPTWTIVNSFTWSIIELHAIILAACIPSFRTLMGRHFPNPLGSSYYSHSTPGSMRMKTKLGRHTHYRVKHHMPYALGSMDTREHTTNHIEGGGGVGGGGGLDDKDRDEGGGMMVVGGDGESEILFIEVPDGGIIKTTEVEVRVVERG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4M1</accession>
    <name>D5G4M1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">26S proteasome regulatory subunit 8 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00000006001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429989">
        <property type="protein sequence ID" value="CAZ79464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835307.1">
        <property type="nucleotide sequence ID" value="XM_002835261.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4M1"/>
    <dbReference type="FunCoup" id="D5G4M1">
        <property type="interactions" value="992"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G4M1"/>
    <dbReference type="GeneID" id="9188401"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000006001"/>
    <dbReference type="eggNOG" id="KOG0728">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_2_0_1"/>
    <dbReference type="InParanoid" id="D5G4M1"/>
    <dbReference type="OMA" id="REPAVIF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19502">
        <property type="entry name" value="RecA-like_PAN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000006">
        <property type="entry name" value="26S protease regulatory subunit 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000068">
        <property type="entry name" value="26S protease regulatory subunit 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000030">
        <property type="entry name" value="26S protease regulatory subunit 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032501">
        <property type="entry name" value="Prot_ATP_ID_OB_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16450">
        <property type="entry name" value="Prot_ATP_ID_OB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0647">Proteasome</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="11">
        <location>
            <begin position="165"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="11"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00069286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ79464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="389" mass="43643" checksum="732E29E0DD8C269E" modified="2010-06-15" version="1">MALNSFFHNKIESLELEIIERNSKLRRLEAQRNDYNSRVRLLREELGLLQQPGSYVGEVVKVMGSKRVLVKVHPEGKYVVDVADSVDISKLTPGKRVTLLSDTYRLEKMLPSSVDPLVSLMMVEKVPDSTYDMIGGLDQQIKEIKEVIELGLKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAHHTDCRFIRVSGSELVQKYIGEGSRMVRELFVMAREHAPSIIFMDEIDSIGSSRVEGSSGGDSEVQRTMLELLNQLDGFEPTKNIKVIMATNRLDILDPALLRPGRIDRKIEFPPPTVEARADILRIHSRSMNLTRGIDLKKIADKMNGCSGAELKGVCTEAGMYALRERRIHVTQEDFDLATAKVLNKHDDKEMSLSKLWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4S4</accession>
    <name>D5G4S4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00000077001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429990">
        <property type="protein sequence ID" value="CAZ79510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835353.1">
        <property type="nucleotide sequence ID" value="XM_002835307.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4S4"/>
    <dbReference type="GeneID" id="9188446"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000077001"/>
    <dbReference type="HOGENOM" id="CLU_1215554_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4S4"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="133"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="228" mass="24412" checksum="C7E061DFD29A5892" modified="2010-06-15" version="1">MSDDGYSDTYSDDFAPYDDYYEDDDLAALVDAMAETAFAQGDPIWDDFDVDYDTTEYHSDWEEYYSDGEFYEEEVVVPNMAGGGGKGGGVDGKKSEVVGTGVVERGRGGGPRAGRRMPPRKRRKIEEKGVGVVGVSVGATVMGTGEDEAEDGGRKERPTGANPITATEGKKNTTVIVPVAKEEKRNGTVIVGKEKDGPAKAEQGAGAPKLDTRKRKPSVDGNEILSID</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>D5G534</accession>
    <name>D5G534_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">asparagine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00000298001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.22" evidence="2"/>
    <dbReference type="EMBL" id="FN429996">
        <property type="protein sequence ID" value="CAZ79665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835508.1">
        <property type="nucleotide sequence ID" value="XM_002835462.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G534"/>
    <dbReference type="FunCoup" id="D5G534">
        <property type="interactions" value="468"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G534"/>
    <dbReference type="GeneID" id="9186362"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000298001"/>
    <dbReference type="eggNOG" id="KOG0554">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004553_2_0_1"/>
    <dbReference type="InParanoid" id="D5G534"/>
    <dbReference type="OMA" id="PEMAFYD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004816">
        <property type="term" value="F:asparagine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070145">
        <property type="term" value="P:mitochondrial asparaginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00776">
        <property type="entry name" value="AsxRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04318">
        <property type="entry name" value="EcAsnRS_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004522">
        <property type="entry name" value="Asn-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00457">
        <property type="entry name" value="asnS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003037">
        <property type="entry name" value="PRK03932.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594:SF34">
        <property type="entry name" value="ASPARAGINE--TRNA LIGASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594">
        <property type="entry name" value="ASPARTYL/LYSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="8">
        <location>
            <begin position="160"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ79665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="492" mass="54911" checksum="6B8EC6256B26DC9E" modified="2010-06-15" version="1">MLPLRPIVRRILSRSFQTCHYSSTSIFPRTISSLLHSPLSDERANETVTVAGWVRSVRRMKNVSFAHVSDGSTIHPLQAVLTKEQSENLSMGTSVKITGEWKESVGGKGQQSKELLAEKVEVLGKADPLTYPLQKKYQTPEFLRTLLHLRQRIPANTNLLHLRSNIISTLTAFFVEHSFIQCHTPIITSSDCEGAGEVFAIVPEKFFNTPKYLTVSGQLHLEALAAAVPRVWTLSPSFRAEKSGTTRHLSEFYMLEVEAAFIDTLDPLVALIEDMVKTIVRSISTSRVGKETLSTAGEDKAVQLEERWKGLLERPWSRITYTEAISRLGLAAEQKEVQFDIPAVWGAALQAEHERFLAQTVFNGPVFVTDYPKSLKPFYMLPSVSTVPTPGEGETVACFDLLVPAVGELIGGSLREYRYDELLTEMRRHGIAVGDGGEDEGSLRWYLELRKWGSTRHGGFGLGLDRLVCYLAGVENIREATAFPRWVGRCDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>D5G567</accession>
    <name>D5G567_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">1-phosphatidylinositol-4-phosphate 5-kinase</fullName>
            <ecNumber evidence="2">2.7.1.68</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">1-phosphatidylinositol 4-phosphate kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Diphosphoinositide kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">PIP5K</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00000277001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14425"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58178"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.68"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.1.68" evidence="2"/>
    <dbReference type="EMBL" id="FN429996">
        <property type="protein sequence ID" value="CAZ79652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835495.1">
        <property type="nucleotide sequence ID" value="XM_002835449.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G567"/>
    <dbReference type="FunCoup" id="D5G567">
        <property type="interactions" value="198"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G567"/>
    <dbReference type="GeneID" id="9186350"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000277001"/>
    <dbReference type="eggNOG" id="KOG0229">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004312_0_3_1"/>
    <dbReference type="InParanoid" id="D5G567"/>
    <dbReference type="OMA" id="WTNWPNG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052811">
        <property type="term" value="F:1-phosphatidylinositol-3-phosphate 4-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016308">
        <property type="term" value="F:1-phosphatidylinositol-4-phosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902635">
        <property type="term" value="P:1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902412">
        <property type="term" value="P:regulation of mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17303">
        <property type="entry name" value="PIPKc_PIP5K_yeast_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.800.10:FF:000009">
        <property type="entry name" value="Phosphatidylinositol 4-phosphate 5-kinase its3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.810.10">
        <property type="entry name" value="2-Layer Sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.800.10">
        <property type="entry name" value="Phosphatidylinositol Phosphate Kinase II Beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027483">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002498">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027484">
        <property type="entry name" value="PInositol-4-P-5-kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023610">
        <property type="entry name" value="PInositol-4/5-P-5/4-kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086:SF8">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 5-PHOSPHATE 4-KINASE, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01504">
        <property type="entry name" value="PIP5K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00330">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51455">
        <property type="entry name" value="PIPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PIPK" evidence="13">
        <location>
            <begin position="227"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="643"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="49"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="90"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="101"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="643"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="721"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="752"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="769"/>
            <end position="787"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00078403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00080374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00082306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ79652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="805" mass="89152" checksum="F861EF3246972B8C" modified="2010-06-15" version="1">MSTFANGGPADRDGRMAVGMGSGAVKGPTTPSPSPENLYFPNGPAVEGGSNSSPNGVQSSAATHGSSSSVASPNSTVSLVAPKTTDHLSAPNSQPNTSRFSSPLPLSASSAAHVGQPQQPPPPGSSLRHRHTLQVPKAYNARNPPPGGVPDAVASGRFSPTTTTSAPRRASISLGRRATRSANSDMHIDEIPQDEDAARWAEAIRAKRASKRRKQEEEEEDDKVVVGTKVDHNHVNWVTAYNMLTGIRFVVSRVNAKMDRPLTDADFDAKHKFSFDITGNELTPSAKYDFKFKDYSPWVFRHLRTKFGLDPADYLMSLTSKYILSELGSPGKSGSFFYFSRDYKYIIKTIHHGEHLFLRKILRDYWKHVEDNPDTLISQFYGLHRVKTPFGRKIHFVVMNNLFPPHRDIHQTFDLKGSTIGRDFKEENLADNPRATLKDLNWLRRDMNLNFGPYKKELFLQQVERDVKLLRKLHIMDYSLLLGIHDLTRGNRDDIRNNALKVFQPGGSESDELHGHMLQRTPSKMEKARQARELRKIINKEVPVSLDRTSNRLPDEFLEQRKTVFYGDDGGFRATHENDEPGDVIYYLGIIDCLTHYGFAKKAEHFWRGLSNPKDQVSAIPPQGYGDRFFKFISGLVKSPEEAARDREMREKANAEAVPARSESDAVDGSRNQAERAGAGERDVPTRSLGTVRSPSAERAGGAAGAVLPVVEEAGEGGSTGARSRSRNGSMSDGGRHSGYFDSVTDRRPSREIASSSSSPATSRKPQRQLDNGTTLRQPLNLQTGSIVQKHEQDDFAIRVARVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5A5</accession>
    <name>D5G5A5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00004247001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the partitioning of the mitochondrial organelle and mitochondrial DNA (mtDNA) inheritance.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the misato family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835541.1">
        <property type="nucleotide sequence ID" value="XM_002835495.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5A5"/>
    <dbReference type="FunCoup" id="D5G5A5">
        <property type="interactions" value="63"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G5A5"/>
    <dbReference type="GeneID" id="9188117"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004247001"/>
    <dbReference type="eggNOG" id="KOG2530">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022511_2_0_1"/>
    <dbReference type="InParanoid" id="D5G5A5"/>
    <dbReference type="OMA" id="SYETGWM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06060">
        <property type="entry name" value="misato"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049942">
        <property type="entry name" value="DML1/Misato"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029209">
        <property type="entry name" value="DML1/Misato_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019605">
        <property type="entry name" value="Misato_II_tubulin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13391">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION REGULATOR MISATO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13391:SF0">
        <property type="entry name" value="PROTEIN MISATO HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10644">
        <property type="entry name" value="Misat_Tub_SegII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14881">
        <property type="entry name" value="Tubulin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Misato Segment II tubulin-like" evidence="5">
        <location>
            <begin position="2"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="DML1/Misato tubulin" evidence="6">
        <location>
            <begin position="123"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF10644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF14881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ79698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="520" mass="58103" checksum="BF2D519B6A3556A6" modified="2010-06-15" version="1">MHEIITLQLGHQANYLGTHFWNTQESYFTYAEDASPSPVDHNVHFRPGIGVDGGETFTPRTLIYDLKGGFGSMKKINALYEVERDSDAGIPQGLWDNGMTIQREPLVEPIEYQRSLDTGLLPARLEPASVRYWSDFNKVFYHPRSSIQLNQYDLNSKVMPFENISLGRDLFNQLNREHDMLDRDFRLFAEECDQMQGVQIFTSAEDAWGGFAAEYVAALRDEYPKTGIMTWGLQDFEKTTRERQINHTVNLAYTLSNIIPLTSLYIPLGFPQSKLPGYVHLDTALQWHKSALLSVAVESCTLPTRLRDGFGAGRLGKLDDLASLLNVNGGQKIATLSMTVHKPNPSAPRSGEPSLSDKGFGLSWDSGGYEFGGMAMRARGVQSHESHTFAVTEVVRVSNHDRTKDMTAGDSPELPNDHHDAVFERYHTSFTYPVIDSFPSIFSTDRIIPVEGDATGGDQRLAVSARLTTSSQVMPRLKSIKNVVSRVVGVDEREAILNDLGEFIEGFETGWNSGSDDYDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5B8</accession>
    <name>D5G5B8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00004261001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the C/M/P thioester hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835554.1">
        <property type="nucleotide sequence ID" value="XM_002835508.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5B8"/>
    <dbReference type="FunCoup" id="D5G5B8">
        <property type="interactions" value="157"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G5B8"/>
    <dbReference type="GeneID" id="9188130"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004261001"/>
    <dbReference type="eggNOG" id="KOG3016">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032690_3_0_1"/>
    <dbReference type="InParanoid" id="D5G5B8"/>
    <dbReference type="OMA" id="QVWFRTN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047617">
        <property type="term" value="F:fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006637">
        <property type="term" value="P:acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009062">
        <property type="term" value="P:fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03444">
        <property type="entry name" value="Thioesterase_II_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03445">
        <property type="entry name" value="Thioesterase_II_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.210">
        <property type="entry name" value="Acyl-CoA thioesterase, double hotdog domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049450">
        <property type="entry name" value="ACOT8-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042171">
        <property type="entry name" value="Acyl-CoA_hotdog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003703">
        <property type="entry name" value="Acyl_CoA_thio"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049449">
        <property type="entry name" value="TesB_ACOT8-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11066">
        <property type="entry name" value="ACYL-COA THIOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11066:SF34">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13622">
        <property type="entry name" value="4HBT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20789">
        <property type="entry name" value="4HBT_3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA thioesterase-like N-terminal HotDog" evidence="4">
        <location>
            <begin position="55"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA thioesterase-like C-terminal" evidence="5">
        <location>
            <begin position="160"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF20789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ79711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="340" mass="37734" checksum="C65B05B3FDD9206B" modified="2010-06-15" version="1">MGAQRRASDTATIKPPRPADPEKSKIENNLEITTLEEFGEGVFTNTRALYKPVGARGIYGGCVIAQTLAVAQKTVPPGMEIHSMHCYFILAGNATIPIVYRVENVRSGRSYATRSVYAEQRGQAIFTTTCSFTRPISEDTDEPKRKVQHSIKFPKDVPAPEDCPTGAQTIDEWLSGGKVDTEVAELLRKREAGGPFESRRAGIVSDIPGTDPTHKIQRQWIRARGNIFDEEAHLPALAYISDSSLIGTVSRVNPQARHGQIGMMLSLDHTIYFHNARKTRADRWLLMESDSPWAADERALATQRIWDPEVGLLATCYQEGLVRLKDGREGECDNNVISKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5D0</accession>
    <name>D5G5D0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00004274001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835566.1">
        <property type="nucleotide sequence ID" value="XM_002835520.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5D0"/>
    <dbReference type="GeneID" id="9188142"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004274001"/>
    <dbReference type="eggNOG" id="ENOG502SSB8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_903702_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5D0"/>
    <dbReference type="OMA" id="LVAFYWW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF92">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="92"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="127"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="206"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="248"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="7">
        <location>
            <begin position="38"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ79723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="308" mass="34432" checksum="D26515BFC0A4F7A3" modified="2010-06-15" version="1">MPDDINGFIALVAFITVLTIFGTIGRLCILRRRPRTLAVNISDAAFVLFTVLMVATAGIAYDVVLQELRIRRDFGDQAIPHMLFTEGYLKEFFAITLFCTTQVWLLKAAFLGYYWSLRETLTDRIKFLLYAVSAYCLVTYIGVLSFQFAYCHPISTNWSTGSDRCLATLTVPGMILQQWTNITSDIMVLIVPLFTISTLNLGRKDLYALSVVFCIGALSIAASVVRFTQVYYVVMHADNSIEAIRRTLVWAVIEKLFAFTAFCLPCLRVFYRKRHSGSGCLPAEKKTLFSTSSHSNSCRSGGGSGRSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5H0</accession>
    <name>D5G5H0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Aldehyde dehydrogenase</fullName>
            <ecNumber evidence="4">1.2.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00004330001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>an aldehyde + NAD(+) + H2O = a carboxylate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:16185"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:29067"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.2.1.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Alcohol metabolism; ethanol degradation; acetate from ethanol: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.3" evidence="4"/>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835606.1">
        <property type="nucleotide sequence ID" value="XM_002835560.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5H0"/>
    <dbReference type="FunCoup" id="D5G5H0">
        <property type="interactions" value="821"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G5H0"/>
    <dbReference type="GeneID" id="9183749"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004330001"/>
    <dbReference type="eggNOG" id="KOG2450">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_0_1_1"/>
    <dbReference type="InParanoid" id="D5G5H0"/>
    <dbReference type="OMA" id="GQLIMQY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019413">
        <property type="term" value="P:acetate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07091">
        <property type="entry name" value="ALDH_F1-2_Ald2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000011">
        <property type="entry name" value="ALD5p Mitochondrial aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000026">
        <property type="entry name" value="Aldehyde dehydrogenase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000001">
        <property type="entry name" value="Mitochondrial aldehyde dehydrogenase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11699">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="10">
        <location>
            <begin position="32"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ79763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="496" mass="53550" checksum="CE8F752BE7D8CB40" modified="2010-06-15" version="1">MSLSINLELPNGVKYDQPLGLFINGEFVRGVEGKTFEVINPSTEKVICSVNEATEKDVDIAVRAARAAFEGEWHKVTPAQRGKFLCKLADLFEENLLTLASVEALDNGKSVSMAKGDVGAAAACLRYYGGWADKVHGKVIDTEHGKFNYTKQEPIGVCGQIIPWNFPILMWAWKIGPAIATGNVVVLKTAEQTPLSGLVAAELVAKAGFPPGVINILSGFGKTAGAAISSHMDIDKVAFTGSTITGRTIMKAVANSNLKKVTLELGGKSPNIVFDDADIDEAISWVNFGIFYNHGQCCCAGSRIYVQEGIYDTFVEKFKKRIKENVVGDPFENETFQGPQVSQLQFDRIMEYIEHGKKEGAKIEIGGKRHGDVGYFIEPTIFSNVDESMKIMQEEIFGPVASIAKFKTVADAIALGNKTTFGLASAVHTKNLETALTVANSLKAGTVWVNTYNTLHAALPFGGFKESGIGRELGKAALANYTQVKTVSVNMNGALY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5H1</accession>
    <name>D5G5H1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="7">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00004331001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5 11">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 11">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the PPP phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="7"/>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835607.1">
        <property type="nucleotide sequence ID" value="XM_002835561.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5H1"/>
    <dbReference type="FunCoup" id="D5G5H1">
        <property type="interactions" value="857"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G5H1"/>
    <dbReference type="GeneID" id="9183750"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004331001"/>
    <dbReference type="eggNOG" id="KOG0373">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004962_8_1_1"/>
    <dbReference type="InParanoid" id="D5G5H1"/>
    <dbReference type="OMA" id="MCLKVKY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032956">
        <property type="term" value="P:regulation of actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060237">
        <property type="term" value="P:regulation of fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903432">
        <property type="term" value="P:regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07415">
        <property type="entry name" value="MPP_PP2A_PP4_PP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000005">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047129">
        <property type="entry name" value="PPA2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45619">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0464">Manganese</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="8">
        <location>
            <begin position="112"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ79764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="304" mass="34067" checksum="0F96667A05A065BD" modified="2010-06-15" version="1">MATGGGPDEWLEMAKKCKYLSEGDMKRLCELVKECLMEESNIQPVSTPVTVCGDIHGQFYDLMELFQVGGGLPDKVNYVFLGDFVDRGYFSLETFTLLMCLKAKYPDRITLVRGNHESRQITQVYGFYEECQTKYGNASVWKSCCQVFDFLALAAIIDGSVLCVHGGLSPEIRTLDQIRVVARAQEIPHEGAFCDLVWSDPEEVETWAVSPRGAGWLFGDKVATEFNHVNGLTLIARAHQLYHFKNKDVVTVWSAPNYCYRCGNVASIMKLEPDLNPNFTIFSAVPDDQRVVPAGRGGRGEYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5H4</accession>
    <name>D5G5H4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00004336001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835610.1">
        <property type="nucleotide sequence ID" value="XM_002835564.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5H4"/>
    <dbReference type="GeneID" id="9183753"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004336001"/>
    <dbReference type="HOGENOM" id="CLU_2724037_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5H4"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ79767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="72" mass="7528" checksum="460FD64776147720" modified="2010-06-15" version="1">MALFSCGVAGALCGAKTVDGGGAWCVGDVDTPTRTVELDVLIGALFHLDNLYLIWRCSLVWPLLGGSRGSQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5L6</accession>
    <name>D5G5L6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00000372001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429999">
        <property type="protein sequence ID" value="CAZ79809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835652.1">
        <property type="nucleotide sequence ID" value="XM_002835606.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5L6"/>
    <dbReference type="STRING" id="656061.D5G5L6"/>
    <dbReference type="GeneID" id="9183797"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000372001"/>
    <dbReference type="eggNOG" id="ENOG502SU1U">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060781_2_0_1"/>
    <dbReference type="InParanoid" id="D5G5L6"/>
    <dbReference type="OMA" id="VECNRAY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14688">
        <property type="entry name" value="bZIP_YAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42070">
        <property type="entry name" value="FILAMENT ASSOCIATED PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_8G06630)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42070:SF1">
        <property type="entry name" value="FILAMENT ASSOCIATED PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_8G06630)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="239" mass="26212" checksum="68E62B93047657CE" modified="2010-06-15" version="1">MPESRTEKDAQRALRIRDTKRRQRARQKEYTAELAQRLRTLEAQGVQATVEMQVSARRVAEENERLRKLLRAIGVDEDVVNGWTEGQEVTYKLMNKLNNGEDESAVGGQRKQCSAGPCNSNTKPEQYREPTPPPTERDPPTPAEPCPSKTPCKLARALAANPHIDIAQLPTPADGQAEGRTSDKTSCGKATQMLLQFATSEEKVQAVAGVLEAGCVQDGKGGCSVENKVMLEALDRVCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>D5G636</accession>
    <name>D5G636_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00001752001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430007">
        <property type="protein sequence ID" value="CAZ79979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835822.1">
        <property type="nucleotide sequence ID" value="XM_002835776.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G636"/>
    <dbReference type="GeneID" id="9187658"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001752001"/>
    <dbReference type="HOGENOM" id="CLU_1062421_0_0_1"/>
    <dbReference type="InParanoid" id="D5G636"/>
    <dbReference type="OMA" id="WWTELAN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003071995" evidence="1">
        <location>
            <begin position="23"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="262" mass="30089" checksum="E3269784292CD066" modified="2010-06-15" version="1">MVSTFNILLTSLLLLFPTEILGSAGKRSYCKLTRTYTYHDNLGPLFVEHRYTCAFPPSPSISRSPAPSKSYSDHTWGPIRLPPPWDHPAGSQAGDARVAERGSIISEAVDYAQQLRDSQQKHQIVSYGCEYLPDSRRLPQNRYSNLFWGVSQLFNQTCLAPGEMVEFVDYRRPWTADWRFTNLNRGERSICDWYTEMANVGRALHYFCYHTLGRSGDEEFSGGEEIVAFGRDSNERRGQRWCLRYSKKGWGEKLFDEECLGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>D5G638</accession>
    <name>D5G638_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="18">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">GSTUM_00001754001</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>[protein]-with a C-terminal TM segment(out) + ATP + H2O = [protein]-with a C-terminal TM segment(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66168"/>
            <dbReference type="Rhea" id="RHEA-COMP:16963"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:90782"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type V subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430007">
        <property type="protein sequence ID" value="CAZ79981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835824.1">
        <property type="nucleotide sequence ID" value="XM_002835778.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5G638">
        <property type="interactions" value="1073"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G638"/>
    <dbReference type="GeneID" id="9187660"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001754001"/>
    <dbReference type="eggNOG" id="KOG0209">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001828_4_0_1"/>
    <dbReference type="InParanoid" id="D5G638"/>
    <dbReference type="OMA" id="WYYSLFN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140567">
        <property type="term" value="F:membrane protein dislocase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015662">
        <property type="term" value="F:P-type ion transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07543">
        <property type="entry name" value="P-type_ATPase_cation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000049">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000054">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000071">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057255">
        <property type="entry name" value="2TM_P5A-ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006544">
        <property type="entry name" value="P-type_TPase_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047820">
        <property type="entry name" value="P5A-type_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01657">
        <property type="entry name" value="P-ATPase-V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630">
        <property type="entry name" value="CATION-TRANSPORTING ATPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630:SF7">
        <property type="entry name" value="ENDOPLASMIC RETICULUM TRANSMEMBRANE HELIX TRANSLOCASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23143">
        <property type="entry name" value="2TM_P5A-ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01229">
        <property type="entry name" value="COF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="55"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="173"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="337"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="528"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1157"/>
            <end position="1177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1236"/>
            <end position="1256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1276"/>
            <end position="1294"/>
        </location>
    </feature>
    <feature type="domain" description="P5A-ATPase transmembrane helical hairpin" evidence="17">
        <location>
            <begin position="137"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="16">
        <location>
            <begin position="377"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="8"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF23143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAZ79981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1338" mass="148454" checksum="D42740C30B998F80" modified="2010-06-15" version="1">MSKKLVEGGHSISHGCGSSLSSSSSPSFTFCRAPSIFGNLLDTGVHPIPSSSSPLFFLLFYLFRLVTFSLIAGGISYFSVPSFLASLTLSIEPQDKIWDICKYSRSGIFRCPIDPLAMAILVNSPQIASASLLNPLPPLFRLYTWPFLAIYPAFFSIYFPSASYQKYLGSQEWTVLYFGTILSIQALIWLGCHWSIGWKAWITGIKASRVEDAKLIKVIPVENAGSPDICELTRDRSSGREEISFLFQKRRFIYSPATGSFSPLVYPVDIEPKPRLETFQGAQGISEKDVGHLFRHYGTNSFDIPVPTFTELFKEHAVAPFFVFQIFCVGLWCLDEYWYYSLFTLFMLVAFESTVVWQRQRTLNEFRGMSIKPYPIYVHRSGKWKEIQSDQLLPGDLVSVGRTKEDSGVACDMILISGTAIVNEAMLSGESTPLLKDSIALRPSYSEIDIEGTDKNAIVHGGTKVLQITHNTSYPDDAPLENTPDVKPPPDGGALAVVTRTGFETSQGSLVRTMIYSTERVSANNAEALLFILFLLMFAIAASWYVWTEGIQKDRKRSKLLLDCVLIITSVVPPELPMELSLAVNTSLAALGKYAIYCTEPFRIPYAGRVDVCCFDKTGTLTGEDLVVEGIAGLNGGKAGIEKLKKVGNAGRETTLVLATAHALVKLDEGEVVGDPMEKATLNALGWTLGKNDTLTSKATSSAPGTSFAAPAGIVQIKRRFQFSSALKRQSSVASVLLNHQGKKSKVTFVGVKGAPETVRNMLVEVPADYEETFKWFTRRGSRVLALGYKYLSNDSEWGQNKLNALKREEVEHNLIFAGFLVLQCPLKDDAKKTVRMLNESSHRVVMITGDNPLTAVHVAREVEIVDRDVLILDAPEDDKFGNNLVWRSVDEKTIIPVDPSDPIDKEIIATKDICVTGYALSKMQDQPDVVDLLRHTWVYARVSPLQKEFILNGLKSAGYTTLMCGDGTNDVGALKQAHIGVALLNGSEEDLRRISDHYRTTKFKEMYEKQVQLTKRFNQIPPPPPGAQQAAKFADKMMAQVMEAEEDSEPPTIKLGDASVAAPFTSKLANVIAIANIIRQGRCTLVATIQMYKILALNCLISAYSLSVLYLDGIKFGDGQVTISGMLMSVCFLSLSRAKPVEKLSKERPQPNIFNFYIIGSVLGQFAVHIVTLIYISKYVARVEPRDDNVDLEGEFTPSLLNSAIYLLQLIQQISTFAINYQGRPFRESIKENKGMYWGLIGVAGVAFSCATEFIPELNEKLKLVPFSNEFKLRMTTVMILDFGVCWAIEKCLKFLFSDYRPKDIAVRRPDQDAAELERLEKELAEELIVPVKDAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>D5G645</accession>
    <name>D5G645_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00001764001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the avfA family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430007">
        <property type="protein sequence ID" value="CAZ79988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835831.1">
        <property type="nucleotide sequence ID" value="XM_002835785.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G645"/>
    <dbReference type="STRING" id="656061.D5G645"/>
    <dbReference type="GeneID" id="9187667"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001764001"/>
    <dbReference type="eggNOG" id="ENOG502S3UW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066707_0_0_1"/>
    <dbReference type="InParanoid" id="D5G645"/>
    <dbReference type="OMA" id="MLPLYHW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004074">
        <property type="term" value="F:biliverdin reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042602">
        <property type="term" value="F:riboflavin reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051606">
        <property type="entry name" value="Polyketide_Oxido-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43355">
        <property type="entry name" value="FLAVIN REDUCTASE (NADPH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43355:SF2">
        <property type="entry name" value="FLAVIN REDUCTASE (NADPH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="266" mass="28152" checksum="54E10687422A0B71" modified="2010-06-15" version="1">MSEAITTEIKEVAFFGPTGGCALAALKLALESGCRCSALTRRPSHLLTQLSSLPQGTLQNLTITEGTVQTPTAVLETLRSQTNSSLPRKYIISGIGGAPKFGLNPFRPTLDNPHICQEAAEAILSGLRELKGGAAEGEWQQPLLAVISTTGLVPGELPFVMKPLYGMLKIPHEDKVKMEGVVTGAEEGLLKWVVGRAGLLTDGPAGGAVRAGYAGRVRNGSGKWGDVEGVAVGYRISRGDVGRWLFEEVIGQDGGEWVGKRVCLVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>D5G693</accession>
    <name>D5G693_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00001662001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430008">
        <property type="protein sequence ID" value="CAZ80036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835879.1">
        <property type="nucleotide sequence ID" value="XM_002835833.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G693"/>
    <dbReference type="GeneID" id="9187172"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001662001"/>
    <dbReference type="HOGENOM" id="CLU_3377392_0_0_1"/>
    <dbReference type="InParanoid" id="D5G693"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ80036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="34" mass="3946" checksum="2BCB2273B69FD013" modified="2010-06-15" version="1">MFRRRWVKSSLSLSLSLLFTVIALLRGGAQYIYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6D0</accession>
    <name>D5G6D0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00004427001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the biotin--protein ligase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430009">
        <property type="protein sequence ID" value="CAZ80073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835916.1">
        <property type="nucleotide sequence ID" value="XM_002835870.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6D0"/>
    <dbReference type="FunCoup" id="D5G6D0">
        <property type="interactions" value="110"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G6D0"/>
    <dbReference type="GeneID" id="9181158"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004427001"/>
    <dbReference type="eggNOG" id="KOG1536">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006150_1_1_1"/>
    <dbReference type="InParanoid" id="D5G6D0"/>
    <dbReference type="OMA" id="HHAFYSN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004077">
        <property type="term" value="F:biotin--[biotin carboxyl-carrier protein] ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd16442">
        <property type="entry name" value="BPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03144">
        <property type="entry name" value="GATase1_ScBLP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019197">
        <property type="entry name" value="Biotin-prot_ligase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004408">
        <property type="entry name" value="Biotin_CoA_COase_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00121">
        <property type="entry name" value="birA_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12835">
        <property type="entry name" value="BIOTIN PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12835:SF5">
        <property type="entry name" value="BIOTIN--PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03099">
        <property type="entry name" value="BPL_LplA_LipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09825">
        <property type="entry name" value="BPL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="3">
        <location>
            <begin position="359"/>
            <end position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ80073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="649" mass="71630" checksum="7C02D60C315188B2" modified="2010-06-15" version="1">MGRMNVLVYSGTGTTVESVRHCLYSLRRLLSPHYAVIPAAADTLLKEPWETSCALLIIPGGADLGYCRVLNGEGNRRIAQFVRKGGAFIGFCAGGYYGCKMVEFEVGNGKMEVVGSRELQFFPGACRGAAFAGFQYASEKGARAPKIKVEKEALGDSELEGFNSYYNGGGLFVDADKYADGGVEVLARFEEKEKLSVEGGDAAVVYCKIGNGGAILTGPHPEFAAVNLDRSAGDSEYRERIELIEQTDTQREAFLRACLIKLGLSASDEAGAIPTLSLLHLSSTNPADVGHLADRLKEISVVGGEKQKIVGENDTFILKRNPEAFSVEKHVRIHDSGHPSVKETPYFDHTAYFKHLERYRSQSRSSPEIFGSFLLYGEVVTSTNTMLDKNFELLQRLPSGLTAVATLQVAGRGRGSNIWVSPMGALVFSTCIRHPRELSVQAPVVFVQYLVALAIVEAIKTYDIGFGDMPIRLKWPNDIYASNPKNENEFLKIAGILVNSSYANNQFLLVVGCGINTTNNAPTTSLNHILDKLNRTRKSKGLSRLPAFEQERLLAKILVVFEEMYYQFCRSGFAPFEDLYYKHWLHSGQVVRLEMEGGRKARIRGITTDYGLLTADELDESGRVTGRSFTLQSDNNSFDFFRGLLRRKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6J6</accession>
    <name>D5G6J6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00004510001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430009">
        <property type="protein sequence ID" value="CAZ80139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835982.1">
        <property type="nucleotide sequence ID" value="XM_002835936.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6J6"/>
    <dbReference type="FunCoup" id="D5G6J6">
        <property type="interactions" value="147"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G6J6"/>
    <dbReference type="GeneID" id="9181224"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004510001"/>
    <dbReference type="eggNOG" id="KOG2417">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039213_0_0_1"/>
    <dbReference type="InParanoid" id="D5G6J6"/>
    <dbReference type="OMA" id="FSVYCVY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025969">
        <property type="entry name" value="ABA_GPCR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022535">
        <property type="entry name" value="Golgi_pH-regulator_cons_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015672">
        <property type="entry name" value="GPHR/GTG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15948">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 89-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15948:SF0">
        <property type="entry name" value="GOLGI PH REGULATOR A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12430">
        <property type="entry name" value="ABA_GPCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12537">
        <property type="entry name" value="GPHR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="30"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="146"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="191"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="322"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="390"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="431"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="479"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="Golgi pH regulator conserved" evidence="7">
        <location>
            <begin position="186"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Abscisic acid G-protein coupled receptor-like" evidence="6">
        <location>
            <begin position="317"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ80139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="526" mass="58091" checksum="71D2D96BB0438510" modified="2010-06-15" version="1">MLPAEALAKPDLCDDDCVPHYVKHHSTSHLAFSSLLFTATFCIIFLGVLQRLFPVLCGAGRRHAHHENRGQRRIAAITFSTIFGATGVLAELILCEVSEWGEASARKAGFQSCVWVLMICLVAVAPLLEFHSLVEGSRISTWRRRYVVLFEMAIFGVWLWVFWSLGDRLPIRKADQSLWRDSTRTLREECLARVGVIGVSLMALLSGFGCVSSPWHDFTVKTRPITEIDISRAQGGLDATIEMLETKRSRMRLLEKKIRDKKASQEGLMSKVLSSIRGDADAQEHAILAKEVSGLETMRTSLAAEFAELQRRLSLEQQAKSLFGRVLQAISYGFSVYCVYRIIATSLAHIPHLHRNKSSFSQSDPINNVLAIIAKHWDPHLDRVAWSRQIGFALSGVIIAGSLNSAMTTFNMLTRAAPGVMGHAVKGENPNLALFVSQISAVYVLASALLLRSNLPPHMSTVISEALGAPLDPAFVDRWFDALFLGAAGLTLVGMIIGRKSGWWGGAGKHDPFCDEMEQLEAGKRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6M8</accession>
    <name>D5G6M8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Clathrin light chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00002134001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Clathrin is the major protein of the polyhedral coat of coated pits and vesicles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasmic vesicle membrane</location>
            <topology evidence="1 6">Peripheral membrane protein</topology>
            <orientation evidence="1 6">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <location evidence="6">Coated pit</location>
            <topology evidence="6">Peripheral membrane protein</topology>
            <orientation evidence="6">Cytoplasmic side</orientation>
        </subcellularLocation>
        <text evidence="6">Cytoplasmic face of coated pits and vesicles.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the clathrin light chain family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430012">
        <property type="protein sequence ID" value="CAZ80171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836014.1">
        <property type="nucleotide sequence ID" value="XM_002835968.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6M8"/>
    <dbReference type="FunCoup" id="D5G6M8">
        <property type="interactions" value="312"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G6M8"/>
    <dbReference type="GeneID" id="9187197"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002134001"/>
    <dbReference type="eggNOG" id="KOG4031">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069856_0_0_1"/>
    <dbReference type="InParanoid" id="D5G6M8"/>
    <dbReference type="OMA" id="QVIKDWR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030132">
        <property type="term" value="C:clathrin coat of coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030130">
        <property type="term" value="C:clathrin coat of trans-Golgi network vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032050">
        <property type="term" value="F:clathrin heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000996">
        <property type="entry name" value="Clathrin_L-chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10639">
        <property type="entry name" value="CLATHRIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10639:SF7">
        <property type="entry name" value="CLATHRIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01086">
        <property type="entry name" value="Clathrin_lg_ch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0168">Coated pit</keyword>
    <keyword evidence="5 6" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="3 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="56"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="97"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ80171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="240" mass="26643" checksum="7E76CCD7A012019C" modified="2010-06-15" version="1">MASRFPSIEEFDEGQTSATTGGETPLDLGLGGTSGMDFSERERLALGEDAALFANPADSDLLGGDSDAQEKFQQDSYEGTYFPALDNPSIGPGGVITGTSEPYLPNQNTYSGMSIQPEEEEPEVIKQWRERQTLEIQRRDELSETRKRETIEKAQRAVDDFYENYNSKRDKAIEVTRTEAQEFLDSRENTTSGGTSWERIAKLVDLTEKGARGGKSDKTRFREMLLSLKKDEKAPGASGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7K9</accession>
    <name>D5G7K9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00004604001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430031">
        <property type="protein sequence ID" value="CAZ80502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836311.1">
        <property type="nucleotide sequence ID" value="XM_002836265.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7K9"/>
    <dbReference type="GeneID" id="9186981"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004604001"/>
    <dbReference type="eggNOG" id="ENOG502SFK0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_562826_0_0_1"/>
    <dbReference type="InParanoid" id="D5G7K9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="23"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="144"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="485" mass="53650" checksum="1841793E33539C83" modified="2010-06-15" version="1">MPRRRERSMIRSGISTFLHRSRSSSPSPSTSTTSPNRKPRGETALERFTKARNVHDFLTNRAPRAICEKETAILKKKDGGKSDSRSNNNRESFTVLRAQVYKKGECNDFKYSPMLAARVPMIRVDPPDDIPFELKIEELRIDNGVVTPPGSPVEGGSSSASKGPKLRMTIPGIPSIHTPPSPPDSPILLASPTRSPPPPPPSQKAPQGHSPFLTLSDDILLRIYTHSPALSTATTLSETNKRLRTLYTSTALLILQKVLSRTSPAAHRFLNILQTSYTPATYAEYYSVATDTVRTFSSLISARCEYLCPENTLATEEALFNVWSYCTLFAPTSSPHNAQEEWLQRGGFSKEQVLDMLKVWNCISVLLQPFLKEERLARMYGVVTTPKDAEGVEVRVLKELEGFVEGLLARGLDTLLPVVRWGEEEHNQRYAIVVAKGLNKVRSDGAERGRFLKEVAGGVYMQMCISEGQGKSSGGVGGWGPTFEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7X2</accession>
    <name>D5G7X2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00002614001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430033">
        <property type="protein sequence ID" value="CAZ80615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836424.1">
        <property type="nucleotide sequence ID" value="XM_002836378.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7X2"/>
    <dbReference type="FunCoup" id="D5G7X2">
        <property type="interactions" value="38"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G7X2"/>
    <dbReference type="GeneID" id="9181434"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002614001"/>
    <dbReference type="eggNOG" id="KOG0691">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025145_3_1_1"/>
    <dbReference type="InParanoid" id="D5G7X2"/>
    <dbReference type="OMA" id="RPALMDK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016558">
        <property type="term" value="P:protein import into peroxisome matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000028">
        <property type="entry name" value="DnaJ domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026894">
        <property type="entry name" value="DnaJ_X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052814">
        <property type="entry name" value="Peroxisomal_DnaJ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45006">
        <property type="entry name" value="DNAJ-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45006:SF1">
        <property type="entry name" value="DNAJ-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14308">
        <property type="entry name" value="DnaJ-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="3">
        <location>
            <begin position="6"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="120"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="163"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="192"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ80615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="494" mass="55837" checksum="FBFFB7D64F824F81" modified="2010-06-15" version="1">MVKETEYYDALGVSSTASELEVKKAYRKLAIKLHPDKNLDDETAAEKFQAISEAYQVLSNTDLRRQYDKFGKERAVPDSGFEDPGEFFSMIFGGDAFVDWIGEISLMKDLTRTMEITMKDAEEDEIASGDDEKKTPESEAGPSNAGASEKKASAIPVADEETPLTSTLPFTSPPISRASTPRPRGVPTRQMLMDKSEEDARMDAEGMSQEEKDLKRKEKAKKGLSKEQREELAAYEAERRKIRQERVDTLARKLVDRICVWTETDKGGEVTHSFNEKTKYEVENLKMESFGIEILHAIGNTYLSKGASFVKSQKFLGISGFFSRLKDKGNIVKDTWGTISTAIDAQMTMEEMAKMEEKGGEDWTDEKKAEYERKVTGKILAAAWRGSRFEIQSVLREVCDKVLNDKNVPLNKRVERAHALIMIGTIFKNAERDPDEEFPEGNPALEAVIPRIMRRLKSRISMLRLGFLLAELNFMIEWSCFDSHCVSGVLFSFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7Y7</accession>
    <name>D5G7Y7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00002725001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430035">
        <property type="protein sequence ID" value="CAZ80630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7Y7"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002725001"/>
    <dbReference type="HOGENOM" id="CLU_2672896_0_0_1"/>
    <dbReference type="InParanoid" id="D5G7Y7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="75" mass="8476" checksum="8DEA1382721A085A" modified="2010-06-15" version="1">MEETSNSPAPSPPYFDSPLESKAAFLSQNPVGSENLQPSDFSSHKPFLYIQRVLDNHRLLRTQSRYESLPLVKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>D5G820</accession>
    <name>D5G820_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Lysine--tRNA ligase</fullName>
            <ecNumber evidence="3 11">6.1.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 11">Lysyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00002716001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>tRNA(Lys) + L-lysine + ATP = L-lysyl-tRNA(Lys) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:20792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9696"/>
            <dbReference type="Rhea" id="RHEA-COMP:9697"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78529"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.6" evidence="3 11"/>
    <dbReference type="EMBL" id="FN430038">
        <property type="protein sequence ID" value="CAZ80663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836472.1">
        <property type="nucleotide sequence ID" value="XM_002836426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G820"/>
    <dbReference type="FunCoup" id="D5G820">
        <property type="interactions" value="1155"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G820"/>
    <dbReference type="GeneID" id="9184135"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002716001"/>
    <dbReference type="eggNOG" id="KOG1885">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008255_6_0_1"/>
    <dbReference type="InParanoid" id="D5G820"/>
    <dbReference type="OMA" id="DFRNEGM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004824">
        <property type="term" value="F:lysine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006430">
        <property type="term" value="P:lysyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00775">
        <property type="entry name" value="LysRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04322">
        <property type="entry name" value="LysRS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000050">
        <property type="entry name" value="Lysine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000044">
        <property type="entry name" value="Lysine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00252">
        <property type="entry name" value="Lys_tRNA_synth_class2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002313">
        <property type="entry name" value="Lys-tRNA-ligase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034762">
        <property type="entry name" value="Lys-tRNA-ligase_II_bac/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044136">
        <property type="entry name" value="Lys-tRNA-ligase_II_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018149">
        <property type="entry name" value="Lys-tRNA-synth_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00499">
        <property type="entry name" value="lysS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001756">
        <property type="entry name" value="PRK00484.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42918:SF9">
        <property type="entry name" value="LYSINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42918">
        <property type="entry name" value="LYSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039101">
        <property type="entry name" value="LysRS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00982">
        <property type="entry name" value="TRNASYNTHLYS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="13">
        <location>
            <begin position="254"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="27"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ80663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="618" mass="69730" checksum="ED7C8D78CC9B2D52" modified="2010-06-15" version="1">MSEQQPPALAENTPNLHLDEVTGEMVSKSELKKRQKKRENDAKKAGKAVAAAATAPPAGGAAATAVGRQLDDGELTPNQYFEIRSRSINRLRETKAPNPYPHKFHVKDKLEDVIRAHSSTLAKGETKPEVEVRVAGRIHGIRSAGSRLRFYDLRAEGVKIQVVAQGQEAVGDYQAQHEYLHRGDIIGIVGFPGRTNPKKADNPGEFSIFAKEVVLLSPCLHMLPTEHFGLKDQETRYRQRYLDLIMNNSTRDIFVTRSKIVSYVRRYFDERAFVEVETPMMNAIAGGATAKPFITHHNDLNMDLFMRVAPELYLKMLVVGGLERVYEIGRQFRNEGIDLTHNPEFTTCEFYWAYADVNDLLDITEELVSEMVKCITGSYRTKFHTQGGKVYEVDWERPWKRIEMMTALEVATGRKFPPGDQLHTKATNEFLKEVAKAHGIECSPPLTNARLLDKMVGEFIEETCINPTFITGHPQMMSPLAKYHRSQPGLCERFEAFVCKKEIVNAYTELNDPFDQRLRFEEQANQKDQGDDEAQLIDENFCTSLEYGLPPTGGWGMGIDRLVMFLTDNYSIKEVLAFREFFLLPRVELGANGLIASMKDGHSEKKSAAEVVGVQPVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>D5G835</accession>
    <name>D5G835_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00002792001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430039">
        <property type="protein sequence ID" value="CAZ80678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836487.1">
        <property type="nucleotide sequence ID" value="XM_002836441.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G835"/>
    <dbReference type="STRING" id="656061.D5G835"/>
    <dbReference type="GeneID" id="9184151"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002792001"/>
    <dbReference type="eggNOG" id="KOG1816">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012913_0_0_1"/>
    <dbReference type="InParanoid" id="D5G835"/>
    <dbReference type="OMA" id="THLKHHE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034098">
        <property type="term" value="C:VCP-NPL4-UFD1 AAA ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.50">
        <property type="entry name" value="Ubiquitin fusion degradation protein UFD1, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056012">
        <property type="entry name" value="DUF7590"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049439">
        <property type="entry name" value="TRAFD1-XIAF1_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004854">
        <property type="entry name" value="Ufd1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042299">
        <property type="entry name" value="Ufd1-like_Nn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055418">
        <property type="entry name" value="UFD1_N2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12555:SF15">
        <property type="entry name" value="FUSION DEGRADATION PROTEIN (UFD1), PUTATIVE (AFU_ORTHOLOGUE AFUA_4G04640)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12555">
        <property type="entry name" value="UBIQUITIN FUSION DEGRADATON PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24503">
        <property type="entry name" value="DUF7590"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21366">
        <property type="entry name" value="TRAFD1-XIAF1_ZnF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24842">
        <property type="entry name" value="UFD1_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23580">
        <property type="entry name" value="Znf_XAF1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin fusion degradation protein UFD1 N-terminal subdomain 2" evidence="4">
        <location>
            <begin position="156"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7590" evidence="3">
        <location>
            <begin position="258"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="TRAFD1/XAF1 zinc finger" evidence="2">
        <location>
            <begin position="535"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="312"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ80678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="753" mass="83527" checksum="10B472190F9B7BE7" modified="2010-06-15" version="1">MASPVLHWSSQLSVLPSSEISSSLVGDKILLPQSALEQLLAAVSSTQSGSQPTPALTDTHPPQVDISWASAARHERPQPQLPHPLMFRLFNPRNNRFSHAAPLEFSAEEGKIGISPLLRDALGIDEDVEMKGAGEGELVEDEEKRITVVWKDLPRGTYVRLRPLEAGYDEEDWKALLERYLRVNFTTLTVGEILAIGGRTLAIGGKEKAEFRFLIDAMKPEEAVCIVDTDLEVDIEPLSEEQARETLKQRLARKSVGSSGGALKIGQPAKGGVLPGEYHFYELSEWDRKEPLVIELDEVDDGDEVDIFVTTSKHHHKPRQDEHAWGDSGSKYPKTIQINPTNIELVDVERIFIGVHGYAPLDIEEGGEQQAEEDNSPRPYSLRITQTEIQDTEAPTITTPPSPDYKQCRNCTQWVPTRTFILHENFCLRNNLVCPHCHQVSKRGAPNTHWHCQQCTAHGNNTPYSLRKHVSIYHTPRPCPACASSSSPYTAVNLPDLAIHRTTTCPEKLVLCRFCHLLLPQESSSNHAELLLLGLTHHELTCGGRTTNCHLCNRIVRMRDLDAHVRNHDLKRLSTPPPITCRNVNCARTTKGLRHPNPLGLCGSCFGPLYAPGYDPGDTALGRRLERRLIRQAITGCDRVWCRNEMCKTGRANLGIADDPAQGVGVAGVMPLIKKVVQDLKDGGGELGLCVDEGTQKRRHLAERIAVESVLEKDGGWDVCWVVAAVEEVSGAGEEEGAIRAWLDRNAVRRGER</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5G874</accession>
    <name>D5G874_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peroxisomal ATPase PEX1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Peroxin-1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00002916001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430041">
        <property type="protein sequence ID" value="CAZ80717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836526.1">
        <property type="nucleotide sequence ID" value="XM_002836480.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G874"/>
    <dbReference type="FunCoup" id="D5G874">
        <property type="interactions" value="922"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G874"/>
    <dbReference type="GeneID" id="9188319"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002916001"/>
    <dbReference type="eggNOG" id="KOG0733">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_8_3_1"/>
    <dbReference type="InParanoid" id="D5G874"/>
    <dbReference type="OMA" id="AIANKYC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990275">
        <property type="term" value="F:preribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000149">
        <property type="entry name" value="Nuclear valosin-containing protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000365">
        <property type="entry name" value="Ribosome biogenesis ATPase RIX7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF171">
        <property type="entry name" value="NUCLEAR VALOSIN-CONTAINING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="9">
        <location>
            <begin position="191"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="9">
        <location>
            <begin position="469"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="108"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="108"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="131"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00032509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ80717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="703" mass="77498" checksum="C8F1CAAC4BB4B55E" modified="2010-06-15" version="1">MLTMAGNRQSLNKGLERRVLAIVRRYIDEHSVTEGNNGSLYLSVSKVYPYVTTDRSMTRQRRRNLEKMIEKALATVRGEEAKADGLDSDLGEIEELNEEDLIGLKQSNTVNQQVADSTIPTSPAPQKRRERWPAKDGEPTKRQKQDNSREPPKNICLKNIGGREEVINDLLEFIAMPLAHPEVNLHTGVQPPCGVLLHGPPGCGKTMLANAIAREVGLPFIAISAPSIVSGVSGEPEKMLRELFEEARGIAPCLMFMDEIDAITQKRDNTQRDMERRIVAQMLTCMDDLALEKTGGKPVMIIGATNRPDSLDPALRRAGRFDKEIYLGVPDEVGREKILRILCEKLRLIGDFDFKKLAKKTAGFVGADLSALAREAGMSGSLSSIQCFLRAHPHRLTRKQLDPLYITFPDFLTGLTRIQPSSKREGFATVPDVTWADVGALESHKAELQMAIVLPIKKPELFASVGLTAPSGVLLWGPPGCGKTLLAKAIANESGANLISIRGPELLNKYIGESERAVRQVFSRAQASIPCIIFFDELDALAPHRNDNLSESSSRVVNTLLTELDGFNDRKGIYIIAATNRPDMIDPSILRPGRLDTLLLEILKTITKKTPLSNNVDLRAIAEHSRCKNFSGADLAALVKRATTLALRESCFIGVGEVEEGKNQVVVTMEHFEKASANIRPSVSEDRREQYQELATTFGSEGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8D6</accession>
    <name>D5G8D6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00004765001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRP12 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430042">
        <property type="protein sequence ID" value="CAZ80779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836588.1">
        <property type="nucleotide sequence ID" value="XM_002836542.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8D6"/>
    <dbReference type="FunCoup" id="D5G8D6">
        <property type="interactions" value="918"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G8D6"/>
    <dbReference type="GeneID" id="9185999"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004765001"/>
    <dbReference type="eggNOG" id="KOG1248">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003753_1_0_1"/>
    <dbReference type="InParanoid" id="D5G8D6"/>
    <dbReference type="OMA" id="PDQMKHR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012978">
        <property type="entry name" value="HEAT_RRP12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057860">
        <property type="entry name" value="HEAT_RRP12_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052087">
        <property type="entry name" value="RRP12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48287">
        <property type="entry name" value="ARM REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48287:SF1">
        <property type="entry name" value="ARM REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25772">
        <property type="entry name" value="HEAT_RRP12_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08161">
        <property type="entry name" value="RRP12_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RRP12 N-terminal HEAT" evidence="6">
        <location>
            <begin position="12"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="RRP12 HEAT" evidence="5">
        <location>
            <begin position="337"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1042"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1090"/>
            <end position="1144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1157"/>
            <end position="1246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1042"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1164"/>
            <end position="1176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1180"/>
            <end position="1193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ80779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1246" mass="137237" checksum="4306DD9501BA4BDF" modified="2010-06-15" version="1">MSLQAKLEKVRSPNLQNQQQVAVVLSAVEDILQEQKTEFTPTAYFAALLSLLQQATSTSAVLNKNLAASTVYLLDLVTPFAPPPLLRSKFQQILSHLVPTLTAHDADGPLLRSSIGCLENLLIAQDAPAWAIPQRDIGPRRALVGLLSLGLDNRPKVRKRAHEAITKILKNPPPSPSLDHPVAEMCATITLQSVADLAKASEGQRKRNGEHDPRVIHSLQLIKAVAGAGGWPNRKVEGLCEVLLSVSKSTNEYVMRAAFGVFEEMFASLVDGMASAKLPRVIEVITELRPSQNDSQLLPSWLAVIARGYEVYAQVSEGEALVELPSLFETIAAFLGSQAYNIRTSAAQCLISLATSCIPQSSLFDITRSTGRVFERITVTTTNLLSVRFQGAWNQVFEILSTLFDKLRWRSTPLLDEAVKVIGSLRSNEGFQGKKEADEVLGRAIRAVGPDTILSILSLNLIKPAPGTSGRAWMLPILRDWVENTKLRHFREEFVPLSEAFFQKVVDFGEGKEKTVEIKIFETLVGQIWALLPGYCDLPLDLTEAFDQTFAELIANVLYQKVDLRSDICKALQRLVDSNKAVLEVPLEDGENLAAQRRVSKADAKKNIAHLASYSGNMLAVLFNVYSTTLPQYRGFILKCLNSFLSITPDTELTTTFQKVTSMLEATLAEPVRKKSETEAAGDTRMPPMAHTLMDLVITITPYLPSGSHLTLFRIFSVTVDKSEDPQLQKKAYKIIPRLAEAESGKQALIQKSEDLQNFLIDAAEKATSPARRDRLITLSQLTEFLPTTDLHFIPSILSEVVISTKEVNEKARTAAFDLLVLMGEKMKSGGVVINSKVTHMPSDASNVAATLDEYITMVSAGLAGSTPHMISASITALTRILYQFKNDISKPLISEIITTLDLFLASKNREIVRSVLGFIKVCIISLPKETMLPRFNTLIPNLMIWSHEHKAHFKAKVKHIIERMVRRFGYEAVEKHVPEDDKKLVINIRKTRERRKKHKDAIAAPSCDEDGGDVEVDAVPKAKRQSRFASEFEAAIYDSDSDMSCDSDAEVDSTPRRKNKRGGKSGEAYIHEGEDEPLDLLDRKSIANISSTRPQRARFGDLRKSATAKARTNKDGKLILGGEDNDNAERSEDVEMGTGLTPADGVNAYVQAIAGKDALARGQRGRLKYSNRKGRHDGDGDDNSDSEDEGMEIDSGKTGKGLYSSSAGSGGRKKAWKEMSGRKGLGVKGVREGRVGKIPRKKGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8D7</accession>
    <name>D5G8D7_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA-directed RNA polymerase subunit</fullName>
            <ecNumber evidence="7">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00004767001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the RNA polymerase beta' chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="7"/>
    <dbReference type="EMBL" id="FN430042">
        <property type="protein sequence ID" value="CAZ80780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836589.1">
        <property type="nucleotide sequence ID" value="XM_002836543.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8D7"/>
    <dbReference type="STRING" id="656061.D5G8D7"/>
    <dbReference type="GeneID" id="9186000"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004767001"/>
    <dbReference type="eggNOG" id="KOG0262">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000487_2_1_1"/>
    <dbReference type="InParanoid" id="D5G8D7"/>
    <dbReference type="OMA" id="RQAFIYD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="CAFA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="CAFA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="CAFA"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.860.120:FF:000006">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.120">
        <property type="entry name" value="RNA polymerase II, clamp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045867">
        <property type="entry name" value="DNA-dir_RpoC_beta_prime"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000722">
        <property type="entry name" value="RNA_pol_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006592">
        <property type="entry name" value="RNA_pol_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007080">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044893">
        <property type="entry name" value="RNA_pol_Rpb1_clamp_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376:SF11">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04997">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00623">
        <property type="entry name" value="RNA_pol_Rpb1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00663">
        <property type="entry name" value="RPOLA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4 7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RNA polymerase N-terminal" evidence="8">
        <location>
            <begin position="329"/>
            <end position="544"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ80780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="544" mass="61413" checksum="1550F4396A0E284E" modified="2010-06-15" version="1">MNISNPVSSEVTGVNFTFLCKDDITKLSVKQVVNPATFETVIPGNVPVPVVGGLYDPALGASDALRQRCLTCHLDYKYCPGHVGHISLPVPVYHPLFFDQMLKMLRSSCLYCFHFRMARAEVNRFECKLKLIQYGLLVEAAELDDIHPNTRGEEEGEEGEFSGSTEMEAFYAKREKFVARAIRRHLRSGGLDEGKVMSVAEERRKVVKEFLSTIQFPRKCNRCQAISPGFRKDGYSKIFEVPLTPKAQSQNVQYGFQRPNAAMMQKKPNRLPETKNHDLNRKGAAGRLLHSMEVLNNLSRLFEKEREILQLLYQPREAISKTPKPLTPEMFFIHAIAVPPTKFRPPQAAGGEISESATNKQLVKILQGCLAIRDYNDKFNNPEIEAGAKTVIISKLMQAFVTLQDDVNSFLDSSKSPLTGAAARTLEEGIKQKLEKKEGLFRMNMMGKRVNFAARSVISPDPNIETREIGVPPVFASKLTYPEPVTEINHQWLMRLRIHLSKTLLTPDALSLSDSKSKRVHRHLLNGDVVLMNRTYDPYALRQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8F2</accession>
    <name>D5G8F2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FAD synthase</fullName>
            <ecNumber evidence="2">2.7.7.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">FAD pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">FMN adenylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">GSTUM_00004794001</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>FMN + ATP + H(+) = FAD + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:17237"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="2.7.7.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; FAD biosynthesis; FAD from FMN: step 1/1.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.2" evidence="2"/>
    <dbReference type="EMBL" id="FN430042">
        <property type="protein sequence ID" value="CAZ80795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836604.1">
        <property type="nucleotide sequence ID" value="XM_002836558.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8F2"/>
    <dbReference type="FunCoup" id="D5G8F2">
        <property type="interactions" value="142"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G8F2"/>
    <dbReference type="GeneID" id="9186015"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004794001"/>
    <dbReference type="eggNOG" id="KOG2644">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056971_0_1_1"/>
    <dbReference type="InParanoid" id="D5G8F2"/>
    <dbReference type="OMA" id="EEFVQWS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003919">
        <property type="term" value="F:FMN adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006747">
        <property type="term" value="P:FAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23948">
        <property type="entry name" value="FAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000187">
        <property type="entry name" value="Probable FAD synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002500">
        <property type="entry name" value="PAPS_reduct_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23293:SF9">
        <property type="entry name" value="FAD SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23293">
        <property type="entry name" value="FAD SYNTHETASE-RELATED FMN ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01507">
        <property type="entry name" value="PAPS_reduct"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0288">FMN</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoadenosine phosphosulphate reductase" evidence="14">
        <location>
            <begin position="101"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoadenosine phosphosulphate reductase" evidence="14">
        <location>
            <begin position="180"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAZ80795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="310" mass="34598" checksum="B91FCDCBFDC2153E" modified="2010-06-15" version="1">MENSTSSGGIATATTTATNSTSPSLQKVDAEQNSQTSTAALAPPEESGEDLYTICTRLSQKVHRFLETDFSSELLKGVQRQTAISLGVIEEALQRYKLENLSISFNGGKDCLVLLILFLASLPPHLAPPPKSAPNASGESGEEKINSVYVTTTHPFPQVDAFVQTCITTYHLSLTRYSQSMKSSFERYLQENPNVEAIFVGTRRTDPHGEFLSHFDVTDHGWPKFMRVHPVVDWGYREVWGFLRELQIPYCVLYDMGYTSLGGTKDTHPNPALLEDGTEVLEGECGERKRRFRPAYELVEDGQERLGRDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>D5G927</accession>
    <name>D5G927_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00004957001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430053">
        <property type="protein sequence ID" value="CAZ81020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836829.1">
        <property type="nucleotide sequence ID" value="XM_002836783.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G927"/>
    <dbReference type="GeneID" id="9181303"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004957001"/>
    <dbReference type="eggNOG" id="ENOG502SEJ8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062848_1_0_1"/>
    <dbReference type="InParanoid" id="D5G927"/>
    <dbReference type="OMA" id="VYIVGNW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007138">
        <property type="entry name" value="ABM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42052">
        <property type="entry name" value="ABM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42052:SF1">
        <property type="entry name" value="ABM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51725">
        <property type="entry name" value="ABM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABM" evidence="1">
        <location>
            <begin position="106"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="201" mass="22180" checksum="722BA30DC9B1B19C" modified="2010-06-15" version="1">MPVLEFAIYTLRPPHTPASPTVRSLLSNALAALNSASGHSFILMKQVESPNTIYLVGTWDSISAHNSFLASAENQKLLADAKDVLNVDAMYHAEMDGSVVPLAAPVVGVGRHHVKRGMKEGFEDCFGRVRGVLRGFTGGWEVAGGWRVDEEEGKGEWLLFTGWESVAQHEEFGRSEKFKEYAEIKGFLEGFEVRHAVKYEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5G935</accession>
    <name>D5G935_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00004966001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430053">
        <property type="protein sequence ID" value="CAZ81028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836837.1">
        <property type="nucleotide sequence ID" value="XM_002836791.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G935"/>
    <dbReference type="GeneID" id="9184315"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004966001"/>
    <dbReference type="HOGENOM" id="CLU_2943499_0_0_1"/>
    <dbReference type="InParanoid" id="D5G935"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ81028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="60" mass="6901" checksum="9A6C01390765219D" modified="2010-06-15" version="1">MGGPTLRFQCLIVPFDLVEFPFQVLNPHISFLDVVDIFFSFAQQLFVQSFHIVIELLLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9H3</accession>
    <name>D5G9H3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00003401001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430061">
        <property type="protein sequence ID" value="CAZ81166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836975.1">
        <property type="nucleotide sequence ID" value="XM_002836929.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9H3"/>
    <dbReference type="STRING" id="656061.D5G9H3"/>
    <dbReference type="GeneID" id="9182917"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003401001"/>
    <dbReference type="eggNOG" id="ENOG502QSS6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030508_0_2_1"/>
    <dbReference type="InParanoid" id="D5G9H3"/>
    <dbReference type="OMA" id="FYRPAVW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09083">
        <property type="entry name" value="EEP-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050410">
        <property type="entry name" value="CCR4/nocturin_mRNA_transcr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121">
        <property type="entry name" value="CARBON CATABOLITE REPRESSOR PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121:SF36">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="1">
        <location>
            <begin position="36"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="290" mass="33038" checksum="F9E415756A920C54" modified="2010-06-15" version="1">MLRTNTPPSPLPLRLYTHNIRYATTHPLPHESSWPTRLPRIISSIAYTQHQHPSTLLCLQEVLRTQLLSILSLLPRSYNYIGRARDDGHEAGEYSPVIYDTEVWRLDKWRSKWLSPTPERPSKGWDAACIRILTVGYFTHWESGRRVVVLNTHLDDQGAVARRESARMIVEEVREVLGEGEGRGVVLAGDMNSPEGDDAYKIFTASGSLLRDARADVPPAKRYGHQMTFSGFGNEHEIPQRIDFVFAAEAKEDDEKAIWTITNYSVLENKFDDEIYSSDHRPVVVDLILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9J0</accession>
    <name>D5G9J0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00003346001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430062">
        <property type="protein sequence ID" value="CAZ81183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836992.1">
        <property type="nucleotide sequence ID" value="XM_002836946.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9J0"/>
    <dbReference type="GeneID" id="9184256"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003346001"/>
    <dbReference type="eggNOG" id="ENOG502QTVX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026180_0_1_1"/>
    <dbReference type="InParanoid" id="D5G9J0"/>
    <dbReference type="OMA" id="WEFIQFV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.36.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003806">
        <property type="entry name" value="ATP-grasp_PylC-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02655">
        <property type="entry name" value="ATP-grasp_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="3">
        <location>
            <begin position="136"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="412"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="412"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ81183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="445" mass="50915" checksum="F39A482FC007057F" modified="2010-06-15" version="1">MHCFKSRKKCTTPLNILLSNGRFPVSIDLARQLKLAGHNVYVVDPMHYHVCKFSNSVVKSYWVPAPHVDAEGYTEGVRRAVVDAKIDLIIPMHEELFHLAEQTSDVRDRNNQIVRKRLFAPDFRTLVRLHNKWEFSRFLRKAGCDGPRAWLCRDMDGVRALPHGEMELALKPVYGRAAKNLYHLKPDTPLPDGLDVSPESEYIAQEWISGNRYCSYSILRDGRIFAFSVYPVVDTIDDSSCVYFQSIEHAGIRAYVERIAEMLTGVDGQIAFDFIETGDRLVAIECNPRATSGIHLWSGTPDLAYSFTRSSPAVAKPGARRQIIPGMLMWSKKGAGLGEYLRHMRRLMETKDVIFNKRDLLPSLMQPFLLTSYYEICRERKMNISTMFQWDLVWEPGKEQLENVRRLLAREGRGEVGGEGTKKDVEREESASWWSGSGVRVERKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9N4</accession>
    <name>D5G9N4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00004989001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430064">
        <property type="protein sequence ID" value="CAZ81227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837036.1">
        <property type="nucleotide sequence ID" value="XM_002836990.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9N4"/>
    <dbReference type="STRING" id="656061.D5G9N4"/>
    <dbReference type="GeneID" id="9185364"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004989001"/>
    <dbReference type="eggNOG" id="ENOG502SYWK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1262336_0_0_1"/>
    <dbReference type="InParanoid" id="D5G9N4"/>
    <dbReference type="OMA" id="GAFNWIA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37451">
        <property type="entry name" value="MARVEL DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37451:SF4">
        <property type="entry name" value="MARVEL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="138"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="167"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ81227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="219" mass="24100" checksum="B19004BEF472CBD1" modified="2010-06-15" version="1">MNSYTAQHVVDQPPWIKFIRIAQVSLSVLILALAAFALSAIPYGNTFGTFGFNIFTCMFTLIVGAYLLITPLRAPKLYNCWAAFVLEIFGVFFWLSPFATQASWASLGLLTVDYLDNITKEAEERYARYRAGWRCAGAAAGLGGIVWILFMITLITYSIFLHRHKKKPGKKNLPENGSPAKEGGEGGIPRPGAAEIKGHEMGSVHVVAVGGDYPRPYQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5GA24</accession>
    <name>D5GA24_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00003538001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430071">
        <property type="protein sequence ID" value="CAZ81367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837176.1">
        <property type="nucleotide sequence ID" value="XM_002837130.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GA24"/>
    <dbReference type="GeneID" id="9187065"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003538001"/>
    <dbReference type="eggNOG" id="ENOG502QTE5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018354_10_2_1"/>
    <dbReference type="InParanoid" id="D5GA24"/>
    <dbReference type="OMA" id="AFRWRDC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.462.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="entry name" value="Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012951">
        <property type="entry name" value="BBE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050416">
        <property type="entry name" value="FAD-linked_Oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42973">
        <property type="entry name" value="BINDING OXIDOREDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G17690)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42973:SF39">
        <property type="entry name" value="FAD-BINDING PCMH-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08031">
        <property type="entry name" value="BBE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="6">
        <location>
            <begin position="35"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ81367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="564" mass="63818" checksum="833BAC662EA6F12B" modified="2010-06-15" version="1">MDTIVSFLKYEEIPVYEPGEVEYERSVAVANLLYRYSRPTCVVQPLRSSDVQIIVKQAKDLKIPITIKNGGHSYAGFSTTDYGILLDLVKMNKVSLDMRARTITLQGGAQWGHAYKTLVSGRHDGYIINGGRCPTVGVSGFILGGGLGPFTRSLGMGCDSLKEVTIVTADGREVTVKDSGNPKSNEDRLFWALCGAGGGNFGVVVKLKMSVQELRDPDVVAGRYTWHPDPSEMGDFMATMKDFYTTEWDNYLTMDTSWLCDLQQIKSELAVRFIIYYDGGKEPFDNLIDRGIQNKELAKQLKRRSMPEKSTRFLHETLVSQWSEEITKTFPTNRTYTIYTSFVFKNNKKTIDSATTIIRDEMKAFRDLFPGEQALLQVTWIHSGGVASKKKREATAFRWRDCVYHTYIMLQWEEKWLELNMRGFLQSFKEKLRPLSMMKRAAFINFPDPALPKGTHERAYYGNNRQELQRIKQIWDKDNFFNWSQGVRLPPQKGPAQAPGAGAGGTELAVRSLMAADEDEVIGEETRVDEQAWTDTIAGKHWQTGIPRKNEFEGGILGLTDLGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAB7</accession>
    <name>D5GAB7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00005224001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
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            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
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        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
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        <subcellularLocation>
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        <subcellularLocation>
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        <property type="entry name" value="alpha/beta hydrolase"/>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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        <property type="entry name" value="Prot_kinase_dom"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Protein kinase-like (PK-like)"/>
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        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
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            <begin position="144"/>
            <end position="457"/>
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    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="478"/>
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            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="990" mass="111204" checksum="6573292A627B501F" modified="2010-06-15" version="1">MSTSTKESELRKRILNQRLPTSVERVRFFPDCDLKQMLTYDVVAEVIGESNIPFHDRRGVIKDILDGAQKIFCILVCIRHVNYMHLLLQSGVVDAKLALSKEGLVSFGLPIPAVEEFWHQQWDFIAPIFKSRIQKLDPSCILPFKEEQDLGEGGYGAVSKVVLYRSHQDLVSISGPGDTVVVARKRIKHVSAKTEAYFESERGPLELLKTRGHPHIIKLLGSYYQNDDYNLFFPLAEGSLKDLFDGGRPGFDVDTESFYSSIVGLSSAINHIHNFTYEMGGKNMNFKGYHHDLKPANILIQQGKFVIADFGLSKFKADANTSSTIHKTGTEAYLPPETCSIDPETNFDDRMVGRSVDIWAFGCILAEIATYLLRGSGGVGEFSRRRLTRVSANMKDNSFHAYNEVKEEVREWLKSLEIMAERDPQISRLIQVVWKTLIPDRKKRPTAQAVLEEVSVLDPETATNMELFVSQGRTALPLSPPISPAGSPQSRSQTTSPGSGTLSDRCGIAVLSEGTRDLGLRIDVVALHGLNGDLYTCWQVGDGPIWLRDFLPSDGMHLPHIVSYGYDSERLFTHLSKDNLEKEAHNFLRQIQKRREIQGASTRPIIFLAHSVGGFPLKQAILLAKGSDEYRDLFKSICGVTFFGTPHREHGIFHSMGACLRKISRTASVSEQSRELLRQNMCDNDQLLKAVNHSFETRICTPGSNLRVVNFYETKPTPPGLGVVVDKDAGTMGLNGVDEKIYGLELSHPDLCRYSSETEPSYKVALRGVRLLTRISADEYGSKQAKELEQEVPYINNYQQFSPEIHVNNILPHLPIIPSLQRPSTGSRLTQNFNTTPPHQPTTTPKTAPRTQTKPPPPPFHTPLKKKQPPPPPQPQPQPQARAHTAPPQQSQKKSQTQNKNSINAIEKRLANVGGRKSNIAAEIVARQKERKGLRLRRQERGEQWFSDETRKLEKRITELEARRTEAHNEQERLKLELIGARTVHGHRTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBE8</accession>
    <name>D5GBE8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00000437001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
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        <property type="term" value="C:Swr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:ATP-dependent H2AZ histone chaperone activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
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        <property type="entry name" value="Swc3"/>
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        <property type="entry name" value="Swc3_dom"/>
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        <property type="entry name" value="Znf_GATA"/>
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    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
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        <property type="entry name" value="SWR1-COMPLEX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SWR1-COMPLEX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Swc3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="4">
        <location>
            <begin position="636"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="151"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="307"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="549"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="683"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="770"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="493"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="22"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="151"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="208"/>
            <end position="235"/>
        </location>
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        <location>
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        </location>
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        <location>
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            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="370"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="549"/>
            <end position="572"/>
        </location>
    </feature>
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        <location>
            <begin position="597"/>
            <end position="615"/>
        </location>
    </feature>
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        <location>
            <begin position="616"/>
            <end position="625"/>
        </location>
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        <location>
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        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="770"/>
            <end position="804"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ81841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="854" mass="92525" checksum="1BCC2D4997E2C437" modified="2010-06-15" version="1">MGEKRKRTVAVRESHPSKHRSATPAAAEAPTPEEQTPTPEVPTSFTKDGPLPVIPQRQPGGLLLSDYQSVAESKVMAESLIRSQRKWVDGDIFEKFWIKPLKSRKLDPAELEKNPSKDCMTRLGNCTMSSMPHKFEIKLFGVLEPLDISENTTSGPGFTAHHAQSSGAGVQTPPAHHQVPQQGVARAPDLARTQSSPASVTPVPHQRPTGNPGISPQPSTPGQPSHQGPQLQPSHPAQQDPPRVAPPPAASRPDPVIQALAIRAASDPDLKALMRIVASGKATTEQLKIFQAHIDVLTPVASSQLIRSHAARPSTQTPGPSVTLGSGHTAQAPHQQTHPGHQQRHQQQPQLQHTQQPQPQHQHQHHPQQHHQQQYHQHQTHSQSHSESPQFAHHPLQCTPSRIPVPHPAQTNPKPRPFPQPPKQEITSVVFEFVDPYSQGDRFLFPKHAILEYLKGGTVVRASFILVRKPVDDLDEYYQPITVVLESATPKPLEILQRVVASAENTLKHMEEIMASKKRAEDTFLVFRLRREPGEAIPDKPEMHEMVEASARTLRERRARRESSTPAHDLKNFADTPQAAPPRPRVKTIKKAIPREPTTQPSIPSPSTSAVRTPVPKMPKTPRPPKPPRSRKGRIADPTKSCHICQTSKTSLWRKADIEGENVTVCNACGIKWKTNAQKAAQAATQGATSQPLPTDRASSSSGIVGPATHQLVAGAPQMQCNQRAVGLTANDLSGQQVHAVAYQISSTTAASSAPVPSLQHHPQLAVNLSAPQPSLPQQDGNSQQSSLTGGLQLAGSSGENGAQSLPLVNDTPQGLVSGAADRPPLPLQSIPEKPIDQNSSMVQDSPGGLGDIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>D5GC01</accession>
    <name>D5GC01_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Altered inheritance of mitochondria protein 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00005731001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AIM6 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430098">
        <property type="protein sequence ID" value="CAZ82044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837853.1">
        <property type="nucleotide sequence ID" value="XM_002837807.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GC01"/>
    <dbReference type="FunCoup" id="D5GC01">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="GeneID" id="9181313"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005731001"/>
    <dbReference type="eggNOG" id="ENOG502QVA8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031561_1_0_1"/>
    <dbReference type="InParanoid" id="D5GC01"/>
    <dbReference type="OMA" id="QRVRFWA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051236">
        <property type="entry name" value="HAT_RTT109-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31571">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31571:SF1">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="27"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="145"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ82044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="351" mass="39397" checksum="13D0B6D4303208C2" modified="2010-06-15" version="1">MLAAGTVPPIEGAVGRYNCLGRLIGSFSFFVLFVLCVTQLFSIATTIVRVIYTTNLQTLLLNFPLSKSVYPTSRTRDILPKPIHSHNDYWRRVPAYTAISYGCISIEADVWEYSGELFIGHDVASLTRNRTFRSLYVDPLREMLDSQNPDTEFTHPRKGDDEEPGEPVGGRNGIWDVDPGQTTHLFVDFKTPGHNTLPVVIAHLEALRTPVNYLTYFNGTHVVRGQITVHLTGDAPFDSIVANSTYRDYFYDAPLRDLSNGRYTPNNTIVASGQFGRDVGSVIWGRGMTKEQKHIVRAQIAEAHKRGIMARYWDTPGWPISVRNAVWGVLMQEGVDLLNADDLKAASYLDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GC24</accession>
    <name>D5GC24_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00005781001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430098">
        <property type="protein sequence ID" value="CAZ82067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837876.1">
        <property type="nucleotide sequence ID" value="XM_002837830.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GC24"/>
    <dbReference type="GeneID" id="9181336"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005781001"/>
    <dbReference type="HOGENOM" id="CLU_3242467_0_0_1"/>
    <dbReference type="InParanoid" id="D5GC24"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ82067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="43" mass="4626" checksum="6A5EE4279BF34349" modified="2010-06-15" version="1">MTVCFFFSSLVRSMHVFLGLGLCFCDGNSWSLVGRHAGGSCVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5GC86</accession>
    <name>D5GC86_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EKC/KEOPS complex subunit BUD32</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 12">Atypical Serine/threonine protein kinase BUD32</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">EKC/KEOPS complex subunit bud32</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">GSTUM_00000638001</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. BUD32 has ATPase activity in the context of the EKC/KEOPS complex and likely plays a supporting role to the catalytic subunit KAE1. The EKC/KEOPS complex also promotes both telomere uncapping and telomere elongation. The complex is required for efficient recruitment of transcriptional coactivators.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the EKC/KEOPS complex composed of at least BUD32, CGI121, GON7, KAE1 and PCC1; the whole complex dimerizes.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="FN430105">
        <property type="protein sequence ID" value="CAZ82129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837938.1">
        <property type="nucleotide sequence ID" value="XM_002837892.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GC86"/>
    <dbReference type="STRING" id="656061.D5GC86"/>
    <dbReference type="GeneID" id="9184807"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000638001"/>
    <dbReference type="eggNOG" id="KOG0667">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_362149_0_0_1"/>
    <dbReference type="InParanoid" id="D5GC86"/>
    <dbReference type="OMA" id="HYNANIS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050494">
        <property type="entry name" value="Ser_Thr_dual-spec_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058:SF130">
        <property type="entry name" value="SERINE_THREONINE PROTEIN KINASES-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="97"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="385"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="461"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="535"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="52"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="73"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="387"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="468"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="554"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="670"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00033194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAZ82129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="772" mass="86049" checksum="9456DCDBC2D793BC" modified="2010-06-15" version="1">MPSRVHYVTARQANRRKVEEGEEMMDMPTPLSTPDRWGNGDSRRERDCTLSGEVQNSDNLHSSPPLANRQPEEGEEIAEEGGDEELVLEDALLDSGYRILSVLGRGNFSTTYLAEHVSKDVDASDSSPLVAIKRLKRPFGAIGENEYSLLQYLHSKGQHPRHIICPISCFLAESGHFHLVLERLDSARPVALVRCFCEEVHSKLACPARHLALAKIILQLLSGLLSLHSHNLIHADLTPANMLFLPSSNRIKLIDLGNTIRPEDREAYLDDFGVQSVCYRAPEILLGQGPLSRVMDVWSAGVIAVELLLDGEVIGEGLPEVELVRSHIEDREGMVRRVIELVGSVTEYRYGMYYLDAYDEASFESVPFPPKKKRRKTIERWYVARTAKPEENTPKESPRRPRKGILRSSLTEKTGEVEIVEFLMEMMEVNVRKRKTVKELMRHPWLVGKLLGDWGGVLTRVPNGDDGRSDEELDDVGLGEVDEEEELDEDGFGLAEETTLVNDEETQDELGNSEGASLLVNGHGNLSAIKESILETEEDPRASPPKRAKPLTPQPESRPQSEPRSSLGPQEDSWSRLDIVSASLDPAHQKTSRGTPPRKIKQEGLSETPEITAEPASPPSTDSTPIIKPEPHSQESEIPTMPQNPNTQRPAVDLLSAPIKPKPVDPDITPSSFHSPSPDPSPLSSVVTSPPSPIPLIPTDLIRGRASDIWHRDLAADMVLLLHPFPTHYNANISSPNRMKFLKMKATTTKSCFYNYGSSFRLYYVLHSRLHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCG9</accession>
    <name>D5GCG9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00005885001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430109">
        <property type="protein sequence ID" value="CAZ82212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838021.1">
        <property type="nucleotide sequence ID" value="XM_002837975.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCG9"/>
    <dbReference type="STRING" id="656061.D5GCG9"/>
    <dbReference type="GeneID" id="9186394"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005885001"/>
    <dbReference type="eggNOG" id="KOG3838">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053733_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCG9"/>
    <dbReference type="OMA" id="WSAEFQF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005537">
        <property type="term" value="F:D-mannose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051136">
        <property type="entry name" value="Intracellular_Lectin-GPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005052">
        <property type="entry name" value="Lectin_leg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12223:SF28">
        <property type="entry name" value="LECTIN, MANNOSE BINDING 1 LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12223">
        <property type="entry name" value="VESICULAR MANNOSE-BINDING LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03388">
        <property type="entry name" value="Lectin_leg-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51328">
        <property type="entry name" value="L_LECTIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="428"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="L-type lectin-like" evidence="8">
        <location>
            <begin position="38"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="254"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="254"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="271"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ82212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="461" mass="52188" checksum="8311874BA489C88C" modified="2010-06-15" version="1">MSSYILRPLKLVKSLSAISALLSILLPTFSAVQGHEVYDRDLSIGLVHPFSEDRQTIPGYTIHGSPQILSDRLVLTPPSPGNQRVGLWTTKTNPYHEWTLDVNFRTSGGERPGGSFHVWYTARGAHGKEGMESVYTSKPWDGLALVVDSYGGYGTIRAYLNDGATDYSIHHNVPSLSFGHCEIHYRNRGALTGLRITHSPKNFKVEADGSLCFETTRVRLPTGYHYGISAATSETPDSYELFSILLSSPERIDPSRAKDAHHEGQTQQHQDQFRHQEQEKSHGRTPHEEDYTMYKPEYEDESPEKYKSEHDQFVDVHNRLQGITHHLAAIQSQVGMLYDKVDSLSHKQDGYRAELRETRVPRSQIDGMEEKMKAIEDLVVQIGNAITSKDYSQQFEVLHKTLENHHSNLLYTVPNKVTQALTTNTPRVGFGITLLIIFQLVLVVAYIVYKRRRASSPKKYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCI7</accession>
    <name>D5GCI7_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Adenylate kinase isoenzyme 6 homolog</fullName>
            <shortName evidence="7">AK6</shortName>
            <ecNumber evidence="7">2.7.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Dual activity adenylate kinase/ATPase</fullName>
            <shortName evidence="7">AK/ATPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00005906001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Broad-specificity nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates. Has also ATPase activity. Involved in the late cytoplasmic maturation steps of the 40S ribosomal particles, specifically 18S rRNA maturation. While NMP activity is not required for ribosome maturation, ATPase activity is. Associates transiently with small ribosomal subunit protein uS11. ATP hydrolysis breaks the interaction with uS11. May temporarily remove uS11 from the ribosome to enable a conformational change of the ribosomal RNA that is needed for the final maturation step of the small ribosomal subunit. Its NMP activity may have a role in nuclear energy homeostasis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>AMP + ATP = 2 ADP</text>
            <dbReference type="Rhea" id="RHEA:12973"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with small ribosomal subunit protein uS11. Not a structural component of 43S pre-ribosomes, but transiently interacts with them by binding to uS11.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the adenylate kinase family. AK6 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.3" evidence="7"/>
    <dbReference type="EMBL" id="FN430109">
        <property type="protein sequence ID" value="CAZ82230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838039.1">
        <property type="nucleotide sequence ID" value="XM_002837993.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCI7"/>
    <dbReference type="FunCoup" id="D5GCI7">
        <property type="interactions" value="796"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GCI7"/>
    <dbReference type="GeneID" id="9186412"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005906001"/>
    <dbReference type="eggNOG" id="KOG3347">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079096_3_1_1"/>
    <dbReference type="InParanoid" id="D5GCI7"/>
    <dbReference type="OMA" id="QCEIFGT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:AMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00039">
        <property type="entry name" value="Adenylate_kinase_AK6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020618">
        <property type="entry name" value="Adenyl_kinase_AK6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12595:SF0">
        <property type="entry name" value="ADENYLATE KINASE ISOENZYME 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12595">
        <property type="entry name" value="POS9-ACTIVATING FACTOR FAP7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13238">
        <property type="entry name" value="AAA_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="2 7" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="NMPbind" evidence="7">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="LID" evidence="7">
        <location>
            <begin position="100"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ82230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="166" mass="19426" checksum="44512D721B99AE41" modified="2010-06-15" version="1">MPRTKPNIIITGTPGVGKSCHCEHLAQITELGLKHLSINHIAKERNCYDGYDDELKSHMIDEDKLLDEIEDEVKEGGYIIDWHILLLCYVRIRPFYMIDWRNGEYKKLQENLDAEIMQVILEEAREAYDEEAIIELRSDTTEEIDSNVDRISAWTRHWIQNNPEGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCS9</accession>
    <name>D5GCS9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00006003001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430120">
        <property type="protein sequence ID" value="CAZ82322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838131.1">
        <property type="nucleotide sequence ID" value="XM_002838085.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCS9"/>
    <dbReference type="GeneID" id="9187941"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006003001"/>
    <dbReference type="HOGENOM" id="CLU_3351399_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCS9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ82322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="37" mass="4291" checksum="BB86D1AFAEDE5AEC" modified="2010-06-15" version="1">MRRQRSWAETSGEMGIYESANAKARPNEYEVGWQGLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCZ5</accession>
    <name>D5GCZ5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00006027001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430131">
        <property type="protein sequence ID" value="CAZ82388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838197.1">
        <property type="nucleotide sequence ID" value="XM_002838151.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCZ5"/>
    <dbReference type="FunCoup" id="D5GCZ5">
        <property type="interactions" value="306"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GCZ5"/>
    <dbReference type="GeneID" id="9183851"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006027001"/>
    <dbReference type="eggNOG" id="KOG2241">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009710_6_6_1"/>
    <dbReference type="InParanoid" id="D5GCZ5"/>
    <dbReference type="OMA" id="DIFYFCS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017102">
        <property type="term" value="C:methionyl glutamyl tRNA synthetase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd10304">
        <property type="entry name" value="GST_C_Arc1p_N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053836">
        <property type="entry name" value="Arc1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002547">
        <property type="entry name" value="tRNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051270">
        <property type="entry name" value="Tyrosine-tRNA_ligase_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11586:SF33">
        <property type="entry name" value="AMINOACYL TRNA SYNTHASE COMPLEX-INTERACTING MULTIFUNCTIONAL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11586">
        <property type="entry name" value="TRNA-AMINOACYLATION COFACTOR ARC1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21972">
        <property type="entry name" value="Arc1p_N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01588">
        <property type="entry name" value="tRNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50886">
        <property type="entry name" value="TRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 3" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="TRNA-binding" evidence="5">
        <location>
            <begin position="182"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="108"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="108"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="131"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="144"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ82388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="384" mass="41254" checksum="B5D239DBC02C7B57" modified="2010-06-15" version="1">MADSTSATPVLEETIPQLNGNLKFRTTILGEKLSIADVAVYARIRGQAASWSDEQKTGEKGYRYIIRWVDFVQNTPDLGLQIPPEEKVAVDPSKVLVYLKPEELVKEKKKEGAADGEGSKKKGRGRVAVGEAKEEAEKVKEGVNEAAGKAAAAVASEGKQCKREKREKPKREAHKKEEPVISPSQIDLRVGHIVYCVPHPNADSLFVSTISMGDPEGSPLVTPHSELSLPNIVIAKLTPLPTVRTVCSGLNGLVPIEEMQDRRVVVANLKPVTMHGIRSAAMVLAASPAPVPGEDATAHRKEAVELVAPPKGAIDGERVFFEGYKTEPEAVLNPKKRIWEQIQPGFATTSDLDGKAPGEGPIGRLVTESGGVSKVKSLVGAVVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5GD90</accession>
    <name>D5GD90_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein YIP</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00006096001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Golgi apparatus membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the YIP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="FN430142">
        <property type="protein sequence ID" value="CAZ82483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838292.1">
        <property type="nucleotide sequence ID" value="XM_002838246.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GD90"/>
    <dbReference type="FunCoup" id="D5GD90">
        <property type="interactions" value="955"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GD90"/>
    <dbReference type="GeneID" id="9183947"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006096001"/>
    <dbReference type="eggNOG" id="KOG3103">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074741_3_0_1"/>
    <dbReference type="InParanoid" id="D5GD90"/>
    <dbReference type="OMA" id="PFQANYG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048280">
        <property type="term" value="P:vesicle fusion with Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045231">
        <property type="entry name" value="Yip1/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006977">
        <property type="entry name" value="Yip1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21236">
        <property type="entry name" value="GOLGI MEMBRANE PROTEIN YIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21236:SF2">
        <property type="entry name" value="PROTEIN YIPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04893">
        <property type="entry name" value="Yip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="201"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="232"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Yip1" evidence="8">
        <location>
            <begin position="102"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ82483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="261" mass="27807" checksum="0D25330CCA5510A3" modified="2010-06-15" version="1">MANFFQSPPPQQQPHYGGGGAPNNLQFYPSSYSSQTQSPQGYGFGGMGSIQPGGAVGSGSREHGLTKGWLAAFGTSGYDDEPPLLEELGVNFGHIKMKTLMVLNPMASVDQHMMDDSDVAGPILFYLLFGAFLLLSGKVHFGYIYGVALLGSISLHLILNLMSPPTTSLNYIRSASVLGYCLLPLVFTSALGIGYNMNGFIGYILSAAAIGWCTYSASNMFIAVLRVRDMRLLVAYPLGLFYSVFGIMAIFGGRGGTPPLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDK4</accession>
    <name>D5GDK4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00001059001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430147">
        <property type="protein sequence ID" value="CAZ82597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838406.1">
        <property type="nucleotide sequence ID" value="XM_002838360.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDK4"/>
    <dbReference type="STRING" id="656061.D5GDK4"/>
    <dbReference type="GeneID" id="9181602"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001059001"/>
    <dbReference type="eggNOG" id="KOG1297">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061714_0_0_1"/>
    <dbReference type="InParanoid" id="D5GDK4"/>
    <dbReference type="OMA" id="KKLMYKR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019129">
        <property type="entry name" value="Folate-sensitive_fs_Fra10Ac1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09725">
        <property type="entry name" value="Fra10Ac1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="171"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="196"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="237" mass="27852" checksum="FCC337499E3412F6" modified="2010-06-15" version="1">MPPTSRAFAQSSGATSAYARHLQAASAYVSYYGGTIPTPAKYRNERDILEDHHQFIRTDEEADTEEKRIAKSYYDKLFKEFAMVELARWREKQIAMRWRTKQEVLDGKGQFSCANLSCRNHRSTLDENPDEEGGLRSVELNFEYAERGEKKHALVTARVCEECADKLRYVQRGDKSKRKRSRGSRSPSRGRHHRGRSEGGEESRRRSESPRGRRRRLDPPTRRDDSRRRRRRHHERD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDV7</accession>
    <name>D5GDV7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00006297001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430153">
        <property type="protein sequence ID" value="CAZ82700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838509.1">
        <property type="nucleotide sequence ID" value="XM_002838463.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDV7"/>
    <dbReference type="STRING" id="656061.D5GDV7"/>
    <dbReference type="GeneID" id="9184495"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006297001"/>
    <dbReference type="eggNOG" id="ENOG502SY9C">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_950586_0_0_1"/>
    <dbReference type="InParanoid" id="D5GDV7"/>
    <dbReference type="OMA" id="AIQHHIP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37048">
        <property type="entry name" value="QUESTIONABLE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37048:SF2">
        <property type="entry name" value="QUESTIONABLE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="274"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="190"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="201"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="214"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ82700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="293" mass="32364" checksum="11E513314FD83332" modified="2010-06-15" version="1">MLTAALIGHVYWLPNTSESRGTSVNGGKLNHPVLVLGAEREGKVQVLLITSFGNTTLSVRFPNPTDQLRRKYLPLKVSGAAPHPDNGILLSVIARGEPPLLTKQSYVNLEPFEVEVRLLDSRHGSTCSLPDDMLGLLMKKLRELRPTFSVHQERRVVASSSSPAKRQPPAPPRQTTPAIQHHIPPPPRQATPATRHQPPLAARPPPPLRSPVPTQQHPLLSPQRTYSSPYGTTAPWQDFGQDLERNAPRQSRYTRTVDHHDSGAELDCSVVWDILGYLGIAVAVFVSVRYLFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDV9</accession>
    <name>D5GDV9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">rRNA adenine N(6)-methyltransferase</fullName>
            <ecNumber evidence="7">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00006301001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Mitochondrial transcription factor that confers selective promoter recognition on the core subunit of the yeast mitochondrial RNA polymerase. Interacts with DNA in a non-specific manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="7"/>
    <dbReference type="EMBL" id="FN430153">
        <property type="protein sequence ID" value="CAZ82702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838511.1">
        <property type="nucleotide sequence ID" value="XM_002838465.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDV9"/>
    <dbReference type="GeneID" id="9184497"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006301001"/>
    <dbReference type="eggNOG" id="ENOG502QY7G">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_399122_0_0_1"/>
    <dbReference type="InParanoid" id="D5GDV9"/>
    <dbReference type="OMA" id="AHERENN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034245">
        <property type="term" value="C:mitochondrial DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034246">
        <property type="term" value="F:mitochondrial transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006391">
        <property type="term" value="P:transcription initiation at mitochondrial promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.100">
        <property type="entry name" value="Ribosomal RNA adenine dimethylase-like, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001737">
        <property type="entry name" value="KsgA/Erm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023165">
        <property type="entry name" value="rRNA_Ade_diMease-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727">
        <property type="entry name" value="DIMETHYLADENOSINE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727:SF17">
        <property type="entry name" value="DIMETHYLADENOSINE TRANSFERASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00398">
        <property type="entry name" value="RrnaAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="4 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="295"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="468"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="486"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ82702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="690" mass="79371" checksum="40111FDF64C5F5F3" modified="2010-06-15" version="1">MTAASIFRRSADESIAHLRSLISPYALSRRSKLPPSLVNWQICDPIMDKLNLLAYKKTTIIDMNPNLGVFSAALHERLQPKQHILLEPEEFYGDRMREFQKTYKNSWYIPLDGYDWNTYNQLFSDNPPPPPWPDNFPKPRIQATTSPDDVNSDLLFVGHMGKSEKSERLIAQFINCCALGTWIQQYGRVRFLLWVPDSIKDRYLPRSIASRARPAASAEAVVDITEVASSNVIRTGRGFQKLPEINPDNVEVEEKRLTSIKPQERKVKIKKKLVIRQKMVEDEIKKLVAELCPGLTPTTRGRPKKLPSKEQKKLEETRARVRELVEAIRNEEPILPASIPCKERVTVLNRCDEIRRRFEHKKGAEEEAEKIPEKENKRKWKLRVPRAPGRPRKLPKQGEPPSIAQIEAIEDKIMNYQGLWIQGMEHPPWYYKNDKAEIRKLALEMATLHPALGVDPKQVLQPEDVYETAMGAGGSPPNGDAAVQEPSATSASETNWSSQQPGKTDSMGGKGDKDTAEVWEEYAERWARRHEVKARAQSRDDEIYAVRNRLLNIHNREYEPLPIKPAEDFFPDIPMSLLEITPKKPHPFFSVPNAHERENNWLIFVWLLRHLFVLRASSILNALRSLAPGAENILEHLPADERIPPSKRVRCLGTIELVNLAMAWKRWPFKDPDTQFQHIEEGVGERNRFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>D5GE32</accession>
    <name>D5GE32_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00006352001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430164">
        <property type="protein sequence ID" value="CAZ82775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838584.1">
        <property type="nucleotide sequence ID" value="XM_002838538.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GE32"/>
    <dbReference type="FunCoup" id="D5GE32">
        <property type="interactions" value="833"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GE32"/>
    <dbReference type="GeneID" id="9183107"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006352001"/>
    <dbReference type="eggNOG" id="KOG0282">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022571_2_1_1"/>
    <dbReference type="InParanoid" id="D5GE32"/>
    <dbReference type="OMA" id="TLWHPHE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000191">
        <property type="entry name" value="mRNA splicing factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032847">
        <property type="entry name" value="PRPF17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43979">
        <property type="entry name" value="PRE-MRNA-PROCESSING FACTOR 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43979:SF1">
        <property type="entry name" value="PRE-MRNA-PROCESSING FACTOR 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="281"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="325"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="411"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="543"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="190"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="193"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ82775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="576" mass="64454" checksum="8CCA16F44E893EE2" modified="2010-06-15" version="1">MVLVNYASSDDEEAGPSSSGISNLAITSSTKVIAAPDVSLEDPMQLRQSLIKSTDKKITTNMTYDQLSAPVVGPSNPFLSAENASRKRKNVLTGYAEESAISDSTFRTQQRTFISLGYAKDPSLVANGNSFVGNQDSVVLHGGKDVVQLRPSRQETDAIKRKRTKKGDPSILEGENAYVGPWAKYNEANTLSESEDEDEEEEGEEEYSAPTSNPIVDKAGKEYLDVNTGGIGKETTEFHGSQLRDYQGRTYMHVPQDLDIDLKKEPGSWTNYIPKKLVHTWKGHTKPITALRFFPGSGHLLLSSSSDAKAKIWDVYHDRELLRTYNGHSKAVTDITFNNSGSQFLTASYDRYMKLWDTETGKCLHRFTTGKIPHVIRFNPDPALHHEFVAGMSDKKIIQFDTRTEQVVQEYDHHLGPVNTITFVDENRRFITTSDDKSLRAWEYGIPVPIKFIAEPYMYSMVRASLHPSGKYVAYQSGDNQVVVYAATDKFRQNRKKAFRGHNNAGYAIDVDISPDGQFLMSGDSGGFLCFWDWKTCKMYHKFQASDGPVVAAQWHPQESSKVASAGLDNVIKFWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>D5GE79</accession>
    <name>D5GE79_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="11">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00006415001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430164">
        <property type="protein sequence ID" value="CAZ82822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838631.1">
        <property type="nucleotide sequence ID" value="XM_002838585.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GE79"/>
    <dbReference type="GeneID" id="9185478"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006415001"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_30_12_1"/>
    <dbReference type="InParanoid" id="D5GE79"/>
    <dbReference type="OMA" id="AWCANIL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000026">
        <property type="entry name" value="MFS quinate transporter QutD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF42">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="21"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="66"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="98"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="121"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="156"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="190"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="283"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="324"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="352"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="391"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="422"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="459"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="22"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="496"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="510"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ82822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="532" mass="58054" checksum="ECE51BE40ED248C8" modified="2010-06-15" version="1">MGLHASGTNDPKEVRNWRIHLLAVAVSMSAMAIGYDSSFIGGTQALVSFKRDFGLNVEGQHVRDTLAGNIVSTFQAGTFFGSLLTFPIAERYGRKKGMVLSSIVFCIGAVIMTTSDGRLSMIYAGRAITGLGIGCASLIVPVYIAETSPPSIRGRLVGIFEILSQGGGMLGFWINYIVDRTVNPIGPTQWLVPTSLQLVPGALLFAASLIAPESPRWLCQQDDWDQASINLTLLRTLPRDHEFIKEELAGIKEQIEFEASKGGAETFKGKVKEMCSKGNRNRIGIGLILMACQNLTGVNIITYYSPRIFETLGITGTSTKLFATGFYGVAKTLGMIVFSVWLAERLGRRKGLIYGAFIGSLPMWYIGGYVMRADPAKAAAAGQYSRNGWGYLAMVCVYLYGFIYCMSWQGITWVYCSEIFPIGIRMICVAITTADQWFWSFVVSRTTPYMITSLGYGTYFFFGSLMILMGIWAFLFVPETKGLTLEDMDRLFGVPERSGDSSDGGFLGDGGEKGRMEKGGAASLEKEKGGAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEF0</accession>
    <name>D5GEF0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00006452001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430175">
        <property type="protein sequence ID" value="CAZ82893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838702.1">
        <property type="nucleotide sequence ID" value="XM_002838656.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEF0"/>
    <dbReference type="STRING" id="656061.D5GEF0"/>
    <dbReference type="GeneID" id="9181524"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006452001"/>
    <dbReference type="eggNOG" id="ENOG502RWRE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_921943_0_0_1"/>
    <dbReference type="InParanoid" id="D5GEF0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000424">
        <property type="entry name" value="Primosome_PriB/ssb"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00436">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50935">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="108"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="108"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ82893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="302" mass="32059" checksum="2A70B949757B19B6" modified="2010-06-15" version="1">MSRITISGKLVRTPELSTTSSGRKCIRYTIRSWQGKERTNFFSFLCITENARSIEYLTSIPRGSRVVAEGQFSPYQIANEDGSKVQKYSIFSNYPDLLHVIEKAEPGAAAGEATQAQEASAPTEAAQAQEEPEPTEATQAQEEPEPAEATQVQEASAPTEAAQAQEEPEPTEAAQAQEEPEPTEATQAQEEPEPAEATQVQEASAPTEAAQAQEAPEPTEATQAQEEPEPAEATQVQEASAPTEAAQAQEAPEPAEATQTQEASAPTEAAQAQEAPEPVEAAQAQEAPAPTEGGEAPDVKPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEQ8</accession>
    <name>D5GEQ8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00001335001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430185">
        <property type="protein sequence ID" value="CAZ83001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002838764.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEQ8"/>
    <dbReference type="GeneID" id="9184913"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001335001"/>
    <dbReference type="HOGENOM" id="CLU_1042780_0_0_1"/>
    <dbReference type="InParanoid" id="D5GEQ8"/>
    <dbReference type="OMA" id="CWITKVW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="267" mass="29635" checksum="0636B553AB6B0734" modified="2010-06-15" version="1">MPPLTLEISLSIIYPTTTTPFSDGSVRIDITAPDQAHRGDVVPQHQPPPTILQTGPSHPIYRLPQSLSSGGMKIHLNAYAASGGTPEVMSHRELARSSRTSMIDKDHILYEITKVPVGSDYRLECFRYGKGTGAYGFGLMSFVSGWLSGGVWNVEYKTQASGAKVETSVKIQIDDGGSEHGVWKLIEPVQKVVARERPPREGEYGENGRSIEIFEDDFHGQGIMPGEEWRDFIVACWITKVWREATRKPLFETSVRSSTEAGTLRTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEY7</accession>
    <name>D5GEY7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00006662001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430197">
        <property type="protein sequence ID" value="CAZ83080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838889.1">
        <property type="nucleotide sequence ID" value="XM_002838843.1"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GEY7"/>
    <dbReference type="GeneID" id="9188618"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006662001"/>
    <dbReference type="eggNOG" id="KOG0519">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000263_0_0_1"/>
    <dbReference type="InParanoid" id="D5GEY7"/>
    <dbReference type="OMA" id="WGQKATF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16922">
        <property type="entry name" value="HATPase_EvgS-ArcB-TorS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000010">
        <property type="entry name" value="Sensor histidine kinase RcsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013655">
        <property type="entry name" value="PAS_fold_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339:SF5">
        <property type="entry name" value="HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08447">
        <property type="entry name" value="PAS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PAS" evidence="6">
        <location>
            <begin position="994"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="7">
        <location>
            <begin position="1067"/>
            <end position="1119"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="1300"/>
            <end position="1516"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="1832"/>
            <end position="1954"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="159"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="319"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="396"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="493"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="737"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="844"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1522"/>
            <end position="1549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="12"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="322"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="412"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="493"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="522"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="565"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="743"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="783"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="922"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1528"/>
            <end position="1548"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="1884"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ83080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1966" mass="212927" checksum="4DFB88CDA32F69C1" modified="2010-06-15" version="1">MSTSSSSNTGRVGTGGTGGSAPGSGNGAGGAGGGNGSGEGRGCGQGAHSIEGLKEVFFLLLSNPSPTISLLLLLFSGCIVIRLFGILISRKAVVSARAQTGRKKSRLYQATTVSASSSPGPPDAPEVASVAASTTASSVTATKGFRAGGDVVRSFSGAGATSASRVRHPTDPTPTPSYPFPRMETPRTLSSGSVSAAFDLNMSSGMGPQDKPIPSGTNTPASFAFQPPGAQPIASWENPDFPTPNMYELALLLHSEAGIDAFWSNIVKIATSCYKAERVTLAVPSDLTDLENTPWGQKATFNIAEDDCLSLTYMGDAGCVDDREESSPEEDDGTHDVPIPKEFGSPTQSENKAGWEGLGSKVEHDDDLAEPLHIDMNPDEPFTPVTIGLAESCHDSARESDWLSETSSLSPLEPPQPPPLPPELGRQRRRDMSPSGGADDEIRGRVFKTLQPLNYEADALLDGAGVSRVLQRGKVVVLGREYRDVKTHLEGLEKRRKEAREAEEAAAGKPSPPNVSGGPAKSSVSIPEAASTQGVEKLDLSSVNNGMPQIPSQPPTPLFFAARSESPDKAHSYEEYEQALTSPWSQSPAPSPAPKQDPHENPFFALPKVDEESFDPQVSSPVYSTQERVNAIGFESAWTVIHIPLVHPSQSKHDPASHKKAPIAILSILSPVIPYPRNLIHSLSHFAPLAATAFSLAQSHANVVNQLTHLRYGRPFERKMDVESILTNSFHYPTPPDNFGGASVTSPSEISSSLSKDSSSRVASPGWEGVTLGMTGPHGSGSGSRSWNNSAASTPASPGMERQDEYFGPPPRGGKIFSRQNSGNVPTLSTLRARIPMVELKVRIEDEGTTGSSGTAKGKRRESSHAAQRRRADMGMGSSKFPNHTDLRRGTNPNKTPTQHLGAIAAEGASPPVSPGPEIAHQHRRGRGVPRRKRPGLSPGYPHTLLHSYGADYAATFQSLYATTSLPGAPSGRHGISSKLATSIQPLNEMPPPSNRLLRTIIDSIPVHVFTALPHNGETTWVNARMLDYRGSTVEELIKGPWDALHPDDRGDYVKRWGTAIRKGEAFSHQVRIRRFDGIYRWFMIRAVPLRDSRGIIVHWFGTNMDIHDQRLAEVDAARQSEMAESESKYRSLANSSPQIVFAATSNDGITYANTQWQGYSGQTLPQALQLGFMEHVHPADRHKCALPGPGETLVNGLPCLGNSFKMTAGGGNLGASGSGKFEEEKPTFSTELRLKDKHGNYRWHLVRCVGVETNFGTGDGQWFGTCTDINNHKLLEQRLKEAKDAAQKTMESKTRFLANMSHEIRTPLIGISGMVNFLLDTPLNGEQLDYCHTISQSSDGLLSVINDILDLSKVEAGMMRMNPEWFRINTLIEDANELLSAMAVAKDLELSFIVEDDVPPVVCGDRVRLRQVMLNVIGNAIKFTTKGEVFSRWSVIKDAQVGEGEVMLQFECHDTGPGFDRKDEELMFKPFSQIDGSSTREHGGSGLGLVISRQLVELHGGRMTAKSQKGKGSTFICSSKFRLPRNPTDSSTTPQAAVTPSAITESPGQKRGILESARLNDYRSDTPPGPAFSEGHSLGTLFSASHEPGSAASIRPTGSAVGQVHGLPIRSPPDPAAMRLSLPSGVQQKYMAYTPPQPGQHNSANYHGPPIGSELGRAVSPPMFSILIVSEQHYSRVAIAHHIRVTLPKGIPNQITDVSKFSESKDLIGGEDPVVFTHVVINLPEHLEIIELLTMLLNSSIHSQTTTLVLTNPTQRATIMQGAADVCDRMSLRLQFIYKPIKPSRFGVIFDPANERDASTDRNRDSAQQVVESQKRVFSQMEKEVGNKGYRVLLVEDNRVNQKVLLRFLARVGLEVETASDGEECVERVFEHEPGHYGLILCDLHMPRKDGFQATKEIRLWEQEHKAHRVPIVALSANVMSDVADRCIAAGFSRYVSKPVDFKELSSTIKDLLQDSQEGEFDHIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEZ6</accession>
    <name>D5GEZ6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00006673001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430197">
        <property type="protein sequence ID" value="CAZ83089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838898.1">
        <property type="nucleotide sequence ID" value="XM_002838852.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEZ6"/>
    <dbReference type="STRING" id="656061.D5GEZ6"/>
    <dbReference type="GeneID" id="9188627"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006673001"/>
    <dbReference type="eggNOG" id="KOG2688">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_850439_0_0_1"/>
    <dbReference type="InParanoid" id="D5GEZ6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045114">
        <property type="entry name" value="Csn12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12732:SF8">
        <property type="entry name" value="NUCLEAR MRNA EXPORT PROTEIN THP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12732">
        <property type="entry name" value="UNCHARACTERIZED PROTEASOME COMPONENT REGION PCI-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ83089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="327" mass="36660" checksum="CA1789F28CB72A31" modified="2010-06-15" version="1">MNQIRLCATIHSNIKTASIPSYLDLYPKSEQCAYNYYHGRHYFYADQFVSALSTLEHAYALARPRDQKQRKMIARYMIAAAVILGKFPSDSLLARPECAGLGAMFLPICHAIRLGDFRGFRCALGDEGPDSGRKQFWIRTELYLTLKLRCQILLWRGLVRRAFLLTRPEQTGMRKKGPPTLDMRHILTLARFLHGPERVDNPLFLGEGFHRDEDSDTDSDLGDDETSYYGLRLEMVDVECAIISLLDQGFIRGYIARQSSGPVLVLGKSDAFPPVAELYREERFPEEDVMESDYEGGPFSAHVAPAGGSGGGNVIRLSGVKPIGFKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5GF30</accession>
    <name>D5GF30_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Leucine carboxyl methyltransferase 1</fullName>
            <ecNumber evidence="3 8">2.1.1.233</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00006721001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Methylates the carboxyl group of the C-terminal leucine residue of protein phosphatase 2A catalytic subunits to form alpha-leucine ester residues.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>[phosphatase 2A protein]-C-terminal L-leucine + S-adenosyl-L-methionine = [phosphatase 2A protein]-C-terminal L-leucine methyl ester + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:48544"/>
            <dbReference type="Rhea" id="RHEA-COMP:12134"/>
            <dbReference type="Rhea" id="RHEA-COMP:12135"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90516"/>
            <dbReference type="ChEBI" id="CHEBI:90517"/>
            <dbReference type="EC" id="2.1.1.233"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the methyltransferase superfamily. LCMT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.233" evidence="3 8"/>
    <dbReference type="EMBL" id="FN430197">
        <property type="protein sequence ID" value="CAZ83123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838932.1">
        <property type="nucleotide sequence ID" value="XM_002838886.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GF30"/>
    <dbReference type="FunCoup" id="D5GF30">
        <property type="interactions" value="535"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GF30"/>
    <dbReference type="GeneID" id="9187984"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006721001"/>
    <dbReference type="eggNOG" id="KOG2918">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031312_1_1_1"/>
    <dbReference type="InParanoid" id="D5GF30"/>
    <dbReference type="OMA" id="IIYEPIR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018423">
        <property type="term" value="F:protein C-terminal leucine carboxyl O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016651">
        <property type="entry name" value="LCMT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007213">
        <property type="entry name" value="Ppm1/Ppm2/Tcmp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13600">
        <property type="entry name" value="LEUCINE CARBOXYL METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13600:SF21">
        <property type="entry name" value="LEUCINE CARBOXYL METHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04072">
        <property type="entry name" value="LCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016305">
        <property type="entry name" value="LCM_mtfrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 8" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="169"/>
            <end position="170"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR016305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR016305-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ83123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="337" mass="37307" checksum="3B1FCF073B7088B8" modified="2010-06-15" version="1">MNPVRFPKRDGGSSAEEKDRVVQQTDKDASQLRLSAIEAGYLEDPFAKLFVAGEVQRRYPLINRGTYIRTTAIDKLVNNFLSSSPETPKQIISLGAGSDTRFFRIASANPGVIPNLIYRELDFRAITSRKIASITASPALQSLLSPQGLSVDQINGSLTSKNYSIHPLDIRTLHPSADFSASINTALPTLFISECCLIYLSPTEADDILKWITTDFSSSVGIILYEPIGGDDAFGRVMIQNLAVRGIVLKTLKKYSSLERQIERLRVLGFVDGQLAVSVKGIHDGWVSPRERGRIAGLEMLDEVEEWDMLAAHYCVAWGWRDRSGVFAGWRNLGRGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>D5GF88</accession>
    <name>D5GF88_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00006776001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="CAZ83181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838990.1">
        <property type="nucleotide sequence ID" value="XM_002838944.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GF88"/>
    <dbReference type="GeneID" id="9188037"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006776001"/>
    <dbReference type="HOGENOM" id="CLU_369266_0_0_1"/>
    <dbReference type="InParanoid" id="D5GF88"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046520">
        <property type="entry name" value="DUF6697"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20411">
        <property type="entry name" value="DUF6697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6697" evidence="3">
        <location>
            <begin position="435"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="156"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="226"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="394"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="644"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="121"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="175"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="245"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="266"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="290"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="353"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="646"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="741"/>
            <end position="754"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ83181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="754" mass="82291" checksum="2D145DFD72A371BB" modified="2010-06-15" version="1">MSHFLLHLTPCLLPFPFLSHPSFLILPSFLIIPSFLILPSYPILNNHSTLYNTLTPTHKILTHSSFLRTISEMVFSRAGSGATAVESSKLSMREMFKLITEKLDHIQETQNSILTNFTEKSLAQNREIEELRRLMRDTEDQVKAQELNELRKALGHTEITPSAGGRFNSEALEAPTSTSHGKTGSVQTVEKSKHNGTIADTFETTLSPDLDIPYTAVDQDVEMAMPDVGDSDRENIAPAPPAPLPSSELPGPEATGVLSTTESNKSKKRPVQKMRPTRGKSGRGRGGGRAPVRVSGGDSSSDSDESSSDKSVEAPKIFPGRGGFGKARRAGALLGSRVQPQAKSPAVVGPIAEESESESESDTEVIPSTSGFVAVNQKTAKTPVRNTAPLLASIGPGMPSAPQSSSGKKIPPPSAKVIARDRKLPENLPDKPFLFTADAIKAITGTRPGKIVAQVTKENVLPYEYYIVFTPDLHFKPPKAIGDTCELIDLHNSEGELLASRYPVFKKVDKVHFAFYGEYKLGGRKMVSLSIWQNSDPVVKKKWAEKFIHTGWGSKMLLDKGLRTPGEIRNFTLKDIMEFFNRDDDSKALRLSATSFEFVNFDRKLYDKMVAVAENLLNGGMSAPNVARSSLTTRVPKQSTAVDAVDAVDVDDNDESPPITARKRRKVGTESPAMVQGPDRGRRFPGRARRATQGAQYSVLHLSKAGEHDSTSPVNNQWRVESRKKREPIPAEPSANKDGDGGDDDNMETGVDSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GF97</accession>
    <name>D5GF97_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00006792001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430208">
        <property type="protein sequence ID" value="CAZ83190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838999.1">
        <property type="nucleotide sequence ID" value="XM_002838953.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GF97"/>
    <dbReference type="FunCoup" id="D5GF97">
        <property type="interactions" value="1078"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GF97"/>
    <dbReference type="GeneID" id="9188046"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006792001"/>
    <dbReference type="eggNOG" id="KOG2476">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019955_2_0_1"/>
    <dbReference type="InParanoid" id="D5GF97"/>
    <dbReference type="OMA" id="IVPITHY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061632">
        <property type="term" value="F:RNA lariat debranching enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07380">
        <property type="entry name" value="MPP_CWF19_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040194">
        <property type="entry name" value="Cwf19-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006768">
        <property type="entry name" value="Cwf19-like_C_dom-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006767">
        <property type="entry name" value="Cwf19-like_C_dom-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12072">
        <property type="entry name" value="CWF19, CELL CYCLE CONTROL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12072:SF4">
        <property type="entry name" value="CWF19-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04677">
        <property type="entry name" value="CwfJ_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04676">
        <property type="entry name" value="CwfJ_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cwf19-like C-terminal" evidence="3">
        <location>
            <begin position="313"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="Cwf19-like protein C-terminal" evidence="2">
        <location>
            <begin position="462"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="257"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ83190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="535" mass="58320" checksum="9F5BECFBAB65C747" modified="2010-06-15" version="1">MAAKVLVIGATNGHFIEAFAKITALHAKNNFSLSLLLGDLFSDPSQATSKEAENIERLLHGKIRVPLPIYFGLGQHSLPVPVREKINSSGEVCENLFFLGKKTILNTSDGIRIVALGGRLDAGLVTGETGGIEGETLPFYSDKDAKGLKGANYADLLLTYEWPEGVGNLSALAPPGVKGTGVIAELAAALSPRYHFAAGGEIFWEREPYQNGMRSSDGGSDTKITRFLSVADWGNEKRAKALYAFSINPKDTNISRPASTTACPYREGGGKKRPRQDSTGGASFFWGDHTSGGYERGGRGGKRGRGTLANLSPAESCFFCLSYPQLEKHLIVSIGNEAYVTTAKGPLTNPTTNPSTLPFSSHVLIIPLTHTPTVTAIDDEDSRKSTIEEMTNYRLAIERMLKSRDCGAVTFEVSRANGVHSHWQLIPVPVDKLAAVEEAFKSEALADRIGEFEKRGLDAENEGDYFRIWISGMDGSLVVSLKEGEYFDLQFGRKVLAKVMGLKSVHWRDCAQTFEQEVKDANDFKEAFKSFDFNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFI0</accession>
    <name>D5GFI0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 11">Cystathionine beta-synthase</fullName>
            <ecNumber evidence="4 11">4.2.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00006909001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 11">
            <text>L-homocysteine + L-serine = L,L-cystathionine + H2O</text>
            <dbReference type="Rhea" id="RHEA:10112"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:58161"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
            <dbReference type="EC" id="4.2.1.22"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-homocysteine and L-serine: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the cysteine synthase/cystathionine beta-synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.22" evidence="4 11"/>
    <dbReference type="EMBL" id="FN430208">
        <property type="protein sequence ID" value="CAZ83273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839082.1">
        <property type="nucleotide sequence ID" value="XM_002839036.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFI0"/>
    <dbReference type="FunCoup" id="D5GFI0">
        <property type="interactions" value="827"/>
    </dbReference>
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        <property type="reaction ID" value="UER00201"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="F:cystathionine beta-synthase activity"/>
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        <property type="term" value="P:cysteine biosynthetic process from serine"/>
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    </dbReference>
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        <property type="term" value="P:cysteine biosynthetic process via cystathionine"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Related to CYS4-cystathionine beta-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046353">
        <property type="entry name" value="CBS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050214">
        <property type="entry name" value="Cys_Synth/Cystath_Beta-Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005857">
        <property type="entry name" value="Cysta_beta_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001216">
        <property type="entry name" value="P-phosphate_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01137">
        <property type="entry name" value="cysta_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10314">
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    </dbReference>
    <dbReference type="Pfam" id="PF00571">
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    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CYS_SYNTHASE"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 10 11" id="KW-0129">CBS domain</keyword>
    <keyword evidence="11" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="7 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 11" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="12">
        <location>
            <begin position="356"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00005003"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00007103"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012041"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00026192"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU361204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ83273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="510" mass="55264" checksum="4801AC2461B1EA99" modified="2010-06-15" version="1">MPVSTSENAILNTIVDHIGDTPLVRLNRIPQSLGIKAQVLAKCEFFNAGGSVKDRIAKRMIEEAEREGKIKPGDTLIEPTSGNTGIGLALVGAVKGYRTIITLPEKMSAEKVSVLRALGAEIVRTPTEAAYDSPESHIGVAKRLEKEIPNSIILDQYSNVNNPLAHELGTAEEIWRQTGGKVDAIVAGAGTGGTISGIARGLRKHNPKVTIICADPVGSTLAPGEIDDSNAGYKVEGIGYDFVPDVLDQNIVDTWVKTADRDSFIYARRLIKEEGLLCGGSSGSALTALVDGIKSRPELNQEGKTIVMILPDSVRNYLSKFVDDTWMENNGFIPAKEIEHARETTQWQGARIRDLKLKPVVTVRAHDTAKSAIDIMTEKSFDQLPVLSNSGQLVGLVTLGNILSYISRGRATSKSPVRELMFDFTKLKEDNGITPHSTSTGRKKAGKRKFEEITLDTHLSALSRFFEHNSAGIVTERLKDGGLQVKHVVTKVDLLSYLVRGGKRAPGVTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFT3</accession>
    <name>D5GFT3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007065001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430220">
        <property type="protein sequence ID" value="CAZ83376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839185.1">
        <property type="nucleotide sequence ID" value="XM_002839139.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFT3"/>
    <dbReference type="GeneID" id="9187287"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007065001"/>
    <dbReference type="HOGENOM" id="CLU_1195623_0_0_1"/>
    <dbReference type="InParanoid" id="D5GFT3"/>
    <dbReference type="OMA" id="ICHICRE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="159"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="232" mass="25815" checksum="3B995FD2F697BCBE" modified="2010-06-15" version="1">MKMPSGSKDALSERIHTSLRSFNYHASDCPSCCDPYSVHLSKTLVLCDVGHALAQEAAEALYAKARTTPMGQIDVTFPEGWESVDGLIKSITHRLQGTPSNRIDLEAMKNAKMVSSNAARRIEAERRGKITYVSAEEVSRGSSFAMDLSRRGIVEVASGPAMQRSRSKHYRYPDPSRSSGLDLIATAHDSHRHHHHHHHGRRADSHSSREHGSPRSTGRAVTFNPDIEMRYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>D5GG46</accession>
    <name>D5GG46_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00007176001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430242">
        <property type="protein sequence ID" value="CAZ83489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839298.1">
        <property type="nucleotide sequence ID" value="XM_002839252.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GG46"/>
    <dbReference type="STRING" id="656061.D5GG46"/>
    <dbReference type="GeneID" id="9184551"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007176001"/>
    <dbReference type="eggNOG" id="ENOG502QS1N">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014631_0_0_1"/>
    <dbReference type="InParanoid" id="D5GG46"/>
    <dbReference type="OMA" id="VPNKSIM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005826">
        <property type="term" value="C:actomyosin contractile ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902716">
        <property type="term" value="C:cell cortex of growing cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005078">
        <property type="term" value="F:MAP-kinase scaffold activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022018">
        <property type="entry name" value="GIT1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013724">
        <property type="entry name" value="GIT_SHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039892">
        <property type="entry name" value="Spa2/Sph1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056439">
        <property type="entry name" value="VBS_C3G9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21601:SF0">
        <property type="entry name" value="PROTEIN SPA2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21601">
        <property type="entry name" value="SPA2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12205">
        <property type="entry name" value="GIT1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08518">
        <property type="entry name" value="GIT_SHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23742">
        <property type="entry name" value="VBS_C3G9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00555">
        <property type="entry name" value="GIT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="GIT Spa2 homology (SHD)" evidence="4">
        <location>
            <begin position="121"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="GIT Spa2 homology (SHD)" evidence="4">
        <location>
            <begin position="177"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="146"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="209"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="357"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="413"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="701"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="445"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="58"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="242"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="279"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="416"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ83489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="909" mass="98864" checksum="18D262B975BA42B9" modified="2010-06-15" version="1">MSINGNIPRSALSPVSVAGSDWSGISGYQKRTSDQQYGGPPPRSGSLASGEGMGPPGQRQNVSPPSSIARSSDGTGLYAQSDASSLKDIIHDEEVAAHHSALRKLLQPYLSQSGDSRPNKARDKLLRLSVVQFRELSTDVYDELQRREGVDASSGPNDKYPPYLLPKEKFHPKRNQARQKLSSLAATRFRDLATDVFFELEKRYPKYATELTRERSPTNSVDGRGMAPPGRLGSPGPGRIGSTGPSGRMGSPGPGQMRGPPGSAPGPYGPGPNGLPRMGPGGPSRNGLPGGLGPPGMEGGGSPNRGDAPSSFGRPLPKTFQPGTVIPNKSTMVDKKLIAEYQGKVEILHDKIVGLEGSLEDRDQEIAKLKDEQRRKESGHNRDRQEWENLKRDLEEKLTNAENLNQNIQNELSSVKAEKSANERSLKDQSEDSRQTVGGASDMKYEDLLKQHGELKTELREQEEVTEEVRREAMEFLSQMKSISERADHSFEREEKLTLEIGKLQDELKEWKARYARTKATLRSLRTSSMGLALQSPTSLISKEGGLSDPDGLVKDVHVTRFQISIDELLRTARGANYSQTLEYVKAVVMATRSITEDIDESKTHNDDRLKLKSRVSATANNLTTAAKNHATGGGLSPVSLVDAAASHLTASIVELIKIVKIRPTPQGDLEDDGEDLIISPASAYEVDRAVSVGLVAQKTNGAAKGQPGSRLSGDSVYSPLSSPEHSRDRSRNGSSGGKDQWGSTLSPARSPAFTGRTVGSNFEELRILLETQMVGIVESIQLLLTSIRAGDDMQTLRKHVDNITPVVWKVLQSTENSMQEPGNGTLIGREGWIVANLSKCLQSMSEMSNEGEPIDGPADKEFRGRLASLAFDLVRETKELIRTVEDINSQSRGGAQMMNQIMSDDSLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGC9</accession>
    <name>D5GGC9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00007318001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.</text>
            <dbReference type="EC" id="3.2.1.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>alpha-D-galactosyl-(1-&gt;3)-1D-myo-inositol + sucrose = raffinose + myo-inositol</text>
            <dbReference type="Rhea" id="RHEA:20161"/>
            <dbReference type="ChEBI" id="CHEBI:16634"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
            <dbReference type="ChEBI" id="CHEBI:17505"/>
            <dbReference type="ChEBI" id="CHEBI:17992"/>
            <dbReference type="EC" id="2.4.1.82"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolases 36 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430264">
        <property type="protein sequence ID" value="CAZ83572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839381.1">
        <property type="nucleotide sequence ID" value="XM_002839335.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGC9"/>
    <dbReference type="STRING" id="656061.D5GGC9"/>
    <dbReference type="GeneID" id="9183170"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007318001"/>
    <dbReference type="eggNOG" id="ENOG502QPVE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006630_1_0_1"/>
    <dbReference type="InParanoid" id="D5GGC9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004557">
        <property type="term" value="F:alpha-galactosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047274">
        <property type="term" value="F:galactinol-sucrose galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008811">
        <property type="entry name" value="Glycosyl_hydrolases_36"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31268">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31268:SF32">
        <property type="entry name" value="GALACTINOL--SUCROSE GALACTOSYLTRANSFERASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05691">
        <property type="entry name" value="Raffinose_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00049426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ83572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="638" mass="71674" checksum="09F62D898EF023BA" modified="2010-06-15" version="1">MAQAPLTPVGPSLSWVIAPWMGPLHGTKNISIQKDALLVMFQRAHDGLHVVSLPVSGLDGGSTYLTSDAEEGGRLIVRSMGDHEKSYDEERDVKLLMGFGPDPHKTVACVFNHLKRKIKEKQGGWTDSCMHHFYQGTKSKDSSTYSEDSQERSPWKDGVSYCTWNGLGWDLSENKILNALDDLEKSGIQVSNLVIDDNWQTLAGRGYCFNGTWSAFEANEKFPGGLKGIVTKVRERFPKIKHIGVWHALHGYWDGITPNSALTEKYKTIEVSWRDNVNSITKKLTMVDSEDIERFYDDFYKRVFLSESGIDCVKTDVQCRIDELTSGADKARLAGPYQEAFRKSAIKYFDQRVIYCMSHVPQILYTALLRDDGLKAFLRSATLHPDAMLMYFYPNVPQSHSWHIFANAMNMILFSQLHILPDWDMFQTSLPQYASIHAAARCLSGGPIFITDSPESHDRYLVSSMVSVTPSAEAPPRALRPSEMAYAVDPYLGYRSSRLLCVKNSYLNESGKVHLLGVFNVSGTYIAELIEAFWPRGEWVVRGHNNQNFKKVEGELMVVGLEAGDWEIFTAAVVQDNVAVLGIKDNMTGAAAVSRWSVKTGKEGREIEVELRALGTLGRWIPYKNCSLNLELIVWLAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGD5</accession>
    <name>D5GGD5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Putative lipoate-protein ligase A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00007326001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein lipoylation via exogenous pathway; protein N(6)-(lipoyl)lysine from lipoate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the LplA family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430264">
        <property type="protein sequence ID" value="CAZ83578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839387.1">
        <property type="nucleotide sequence ID" value="XM_002839341.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGD5"/>
    <dbReference type="FunCoup" id="D5GGD5">
        <property type="interactions" value="245"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GGD5"/>
    <dbReference type="GeneID" id="9183176"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007326001"/>
    <dbReference type="eggNOG" id="KOG3159">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022986_3_0_1"/>
    <dbReference type="InParanoid" id="D5GGD5"/>
    <dbReference type="UniPathway" id="UPA00537">
        <property type="reaction ID" value="UER00595"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017118">
        <property type="term" value="F:lipoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16443">
        <property type="entry name" value="LplA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004562">
        <property type="entry name" value="LipoylTrfase_LipoateP_Ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12561">
        <property type="entry name" value="LIPOATE-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12561:SF3">
        <property type="entry name" value="LIPOYLTRANSFERASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21948">
        <property type="entry name" value="LplA-B_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="5">
        <location>
            <begin position="63"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ83578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="321" mass="36012" checksum="C0975890726EBF5B" modified="2010-06-15" version="1">MSPSRGLIFARTVSRAHQRQFRRYQSTFRTFSGSQIPVQVYISKSNNPYLNLSLEHHLFQTTPPKIATLLIYTNSPSIVLGRNQNPWVEVNHPQFASTFNPPLIVRRRSGGGTVFHDLGNVNYSVAMPTSAFCRDKHAEMVVRALRKLGVAEAVVNKRHDIVILPDGMTVEETRLTREVVGTKKVSGSAYKLTTRRSYHHGTMLLESRLEDVRGYLRSAAREWIKARGVDSVRSMVGNVRIEGGKFVGAVVEEFGRMYVGEEGMEILKDAMASRRDRIDGEVGVCWVGEEAGEVEEVQKGIAELMVSSRVYGRVLYAELVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGH7</accession>
    <name>D5GGH7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007388001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430264">
        <property type="protein sequence ID" value="CAZ83620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839429.1">
        <property type="nucleotide sequence ID" value="XM_002839383.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGH7"/>
    <dbReference type="GeneID" id="9183218"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007388001"/>
    <dbReference type="HOGENOM" id="CLU_1653422_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGH7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="160" mass="18002" checksum="368902E538AE8156" modified="2010-06-15" version="1">MPIRRRMGRKSLWVGWQMSRSSLSSGGSTDRGIVTPTPPFLDEGSLAVGIAGWDTSAERAQREALYPRSHPSRLIPPLHRLSFLLGQLGKCYLFSLSVRHHSLLRPGSPLDTSPPYRPPHTIPSARKLDNAEPRRVLVPPASLRIHLRIHLRIHLLHRSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGR3</accession>
    <name>D5GGR3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="11">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00007481001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HAUS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430275">
        <property type="protein sequence ID" value="CAZ83685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839494.1">
        <property type="nucleotide sequence ID" value="XM_002839448.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGR3"/>
    <dbReference type="GeneID" id="9185607"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007481001"/>
    <dbReference type="eggNOG" id="ENOG502S1U0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068908_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGR3"/>
    <dbReference type="OMA" id="QAKDWAY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070652">
        <property type="term" value="C:HAUS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026243">
        <property type="entry name" value="HAUS1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31570">
        <property type="entry name" value="HAUS AUGMIN-LIKE COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31570:SF1">
        <property type="entry name" value="HAUS AUGMIN-LIKE COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25762">
        <property type="entry name" value="HAUS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="7 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="161"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ83685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="298" mass="33775" checksum="9BF4DE0A1C7B5505" modified="2010-06-15" version="1">MADYRDIWSPAKVFSPSAAKHQNHLAKEWAYVDQWLAARYHPKPVPKFERNPDTLKALTTLASASEAANEERALETKVKEKALAELKARDKAASENEESVLIALEENLTPEGTRALNSIALLSVALGSTSTDTQKLAKNLIDLTKEEFELKYQSIRIETLHKRLQKDLAHLREKLNCMEHSEEFKVPPELAGQVAEWTRTGRHLRHKAEDYKERVEMLQNEFNSSRLATGLTVEAVAGQEKAVEALKEHVLNLESQAKCFQGLPPEKDLARLEVERVAKELEELEAKREKLYDTMVGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GH20</accession>
    <name>D5GH20_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00007663001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430297">
        <property type="protein sequence ID" value="CAZ83813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839622.1">
        <property type="nucleotide sequence ID" value="XM_002839576.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GH20"/>
    <dbReference type="STRING" id="656061.D5GH20"/>
    <dbReference type="GeneID" id="9185067"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007663001"/>
    <dbReference type="HOGENOM" id="CLU_1571754_0_0_1"/>
    <dbReference type="InParanoid" id="D5GH20"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ83813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="170" mass="18088" checksum="94EFEB47D7CABC8C" modified="2010-06-15" version="1">MSARLLSMGAVSAAFSRRPLLRAATSRRLLSSPMSTMFASRAPPTSAGLLRISYASRPRTFLRAGQLSRSHSGAAAVRSLSFSRAIPKLVYKFARLPVAFGGATVAGLAYVQYQAQQAGTYAIGLFNSAGEMVGTAIAGAKGTAGDLYGVSRMDARSLELQKIRRGRVWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHB9</accession>
    <name>D5GHB9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00007740001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430308">
        <property type="protein sequence ID" value="CAZ83871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839680.1">
        <property type="nucleotide sequence ID" value="XM_002839634.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHB9"/>
    <dbReference type="GeneID" id="9182576"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007740001"/>
    <dbReference type="HOGENOM" id="CLU_1476187_0_0_1"/>
    <dbReference type="InParanoid" id="D5GHB9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072085" evidence="2">
        <location>
            <begin position="23"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="63"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="142"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ83871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="183" mass="17114" checksum="7D18C016D522ED20" modified="2010-06-15" version="1">MRSSNILAKLLALASIVGIVAAQTFSIVPPPTTTGTGAGAGTSPTTLTPPRPSTPTGPPSSPVAPVTPVTPVTPSGPSIPSATTNTTSRSAGLTTGLSIPSGNSTGPAVASKTGDTAGGSGEEKSGTVSSPTGGSGSGTSGDGSVSPASSSKPATTSSSGAADRNGGSVLAVVVLGSLVWFLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHK4</accession>
    <name>D5GHK4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00007958001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430319">
        <property type="protein sequence ID" value="CAZ83997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839806.1">
        <property type="nucleotide sequence ID" value="XM_002839760.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHK4"/>
    <dbReference type="GeneID" id="9183651"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007958001"/>
    <dbReference type="eggNOG" id="ENOG502QW68">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009650_0_0_1"/>
    <dbReference type="InParanoid" id="D5GHK4"/>
    <dbReference type="OMA" id="FPDAVMG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021822">
        <property type="entry name" value="DUF3405"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36205">
        <property type="entry name" value="CHROMOSOME 19, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36205:SF1">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11885">
        <property type="entry name" value="DUF3405"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="282"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="309"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ83997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="728" mass="82208" checksum="6E2983FA233109BB" modified="2010-06-15" version="1">MSWNPFMKVHSYSPLPLSIPAEPSEKWRFSRNGTVTSRLKRVMCKFVIILTSVTAIIAGFSLVAIRHPTSRVITQAKAVAEKPRWEGFALLEGYYSGMARLVKDNRPEYPPLGDTGVIEDLEVSSEGKSVVFNPYPDFGSKEYRGTWRGTYKECSYGVDLSGKKRGRPEVRTFEGIPEGTGFGHFRVEMRANESVLGMPTAVFGSHSLLGLKDDLCFDRYGRYGSYGYGYSKDNGGLGVAEWDGNITDGGETGWGQGSMPVKEIDWRGIDWGKLQRECVEKNEDRFSASDPEDLDPEGVLAESVHSSNKRSEKSDGKKPRPRAAVLLRTWTGYEYKPSDMASIRSLISELSINSGGEFNVYLLVHVKDNELPIWASKKLYEKTLRENVPEEFWGIAELWSERMMGAIYNNLEDNTFHGARLQGVYRSSFFPVQDFAYRHPEFDFVWNWEMDVRYIGHFYELFSRMDAWAAKQPRKHLWERNERYYVPSVHGSWEDFSSAVAAKSPKPIWGPVPVESVKIFSTDPKPPFLTPEKDTESMWGVDEPADLISLNPIFDPEGATWFLSGDSTGYGPPVEDTPPKGPPRRTAIVAVGRLSRKLLMRMHAENALYGHAMFSEMWPPSVALQHGLKAVFAPHPLFVDRAWPLRYLERTFNGGVKGSAGGRGSVFGKGEHNFQGTSWYYNARFAPKLWKRWVGMEVDGAGGETFESVQGRMCLRSMLLHPIKGMEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHN1</accession>
    <name>D5GHN1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00008055001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430320">
        <property type="protein sequence ID" value="CAZ84061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839870.1">
        <property type="nucleotide sequence ID" value="XM_002839824.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHN1"/>
    <dbReference type="GeneID" id="9183715"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008055001"/>
    <dbReference type="HOGENOM" id="CLU_2724047_0_0_1"/>
    <dbReference type="InParanoid" id="D5GHN1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ84061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="72" mass="7955" checksum="5E2618346E5FE9CC" modified="2010-06-15" version="1">MENALLSQCGRVCLSECPDVSLIPQNQRAYHDRQRVQSMPVFTGMCSEVRNVPVFSLVATGSLLAGWVEDTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHR6</accession>
    <name>D5GHR6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008007001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430320">
        <property type="protein sequence ID" value="CAZ84027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839836.1">
        <property type="nucleotide sequence ID" value="XM_002839790.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHR6"/>
    <dbReference type="STRING" id="656061.D5GHR6"/>
    <dbReference type="GeneID" id="9183681"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008007001"/>
    <dbReference type="eggNOG" id="KOG4055">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079129_4_0_1"/>
    <dbReference type="InParanoid" id="D5GHR6"/>
    <dbReference type="OMA" id="PDREIHM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004860">
        <property type="term" value="F:protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009548">
        <property type="entry name" value="Prkrip1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13507">
        <property type="entry name" value="PRKR-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13507:SF0">
        <property type="entry name" value="PRKR-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06658">
        <property type="entry name" value="DUF1168"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="101" mass="11191" checksum="3289A36EB1A8239F" modified="2010-06-15" version="1">MSEPIPEAIPTSQDPRNKRPTKRRALSPASAQATALTNLFSKPDREIHMPTNPKTKVLPPPPEIVTNVQGSSAGAGSGEFHVYKAARRREYERIRLMEEEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHU7</accession>
    <name>D5GHU7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00008124001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430321">
        <property type="protein sequence ID" value="CAZ84090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839899.1">
        <property type="nucleotide sequence ID" value="XM_002839853.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHU7"/>
    <dbReference type="STRING" id="656061.D5GHU7"/>
    <dbReference type="GeneID" id="9184183"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008124001"/>
    <dbReference type="eggNOG" id="KOG4750">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013964_0_1_1"/>
    <dbReference type="InParanoid" id="D5GHU7"/>
    <dbReference type="OMA" id="VESPFHC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016407">
        <property type="term" value="F:acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03357">
        <property type="entry name" value="LbH_MAT_GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051159">
        <property type="entry name" value="Hexapeptide_acetyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024688">
        <property type="entry name" value="Mac_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23416">
        <property type="entry name" value="SIALIC ACID SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23416:SF76">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14602">
        <property type="entry name" value="Hexapep_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12464">
        <property type="entry name" value="Mac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01266">
        <property type="entry name" value="Mac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="5">
        <location>
            <begin position="53"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="92"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="207"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="29"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="106"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="207"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ84090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="459" mass="49182" checksum="3FF48AA7F4BAE527" modified="2010-06-15" version="1">MNEKHGGNYTVDDEKLNDGDGTRAGASEGSPQPGSQQGQGKRKRVFSNRTKTGCITCRRRKKKCDEGKPECFNCKRGGFVCEGYSGKINWQKPPGRSANAAGAPVSIQSKTEQTGSASASPSPAVVQKQSQTHRLDEVAQHQSPQTRVVQFPYVTTPTVVPEKQVSEGSTSPNTVELPTPHPPPRTQMPQAPALPFVSVVPQQATPTNNAVTSHPTSVSQATVATASGSSMPRERSEKEKMISSELYFASSPELVEERERCKAACWRFNSAATNPDLGISKAEKGRLLHQILRPGGTWEVTRINGLTGPEISYINDAGTPSDIVDVRVDAPFNCSYGYNIRLGRDVLIEAGCTILDSCSVTIKARTVLSPDVSIYSATHPIDPRKRNGSKGPELAKPVMIEEDCWLGGNVIVLGGISIGKGSVVGAGSVVTRDVPPYTVVAGNPARVIRGIYRNDSTDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHV6</accession>
    <name>D5GHV6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00008136001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430321">
        <property type="protein sequence ID" value="CAZ84099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839908.1">
        <property type="nucleotide sequence ID" value="XM_002839862.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHV6"/>
    <dbReference type="GeneID" id="9184192"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008136001"/>
    <dbReference type="HOGENOM" id="CLU_1797853_0_0_1"/>
    <dbReference type="InParanoid" id="D5GHV6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ84099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="144" mass="16409" checksum="6A591C6585669127" modified="2010-06-15" version="1">MRPEDPRLMIGHAEIRHAVSRHRFGGYEGSQCIRMIPDRLGSQRITIRQDGLSLPLMFRLFSRVATYLGVYLSPGLSLASLFTDRLVCRSPAFTSELAMAVLLSLFQHLALSIGFFMHEEWEVEGSDLERYVEQYSIDGDSEHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>D5GI83</accession>
    <name>D5GI83_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008328001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430324">
        <property type="protein sequence ID" value="CAZ84226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840035.1">
        <property type="nucleotide sequence ID" value="XM_002839989.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GI83"/>
    <dbReference type="STRING" id="656061.D5GI83"/>
    <dbReference type="GeneID" id="9181944"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008328001"/>
    <dbReference type="eggNOG" id="ENOG502S16F">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009388_3_0_1"/>
    <dbReference type="InParanoid" id="D5GI83"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010730">
        <property type="entry name" value="HET"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39596">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39596:SF2">
        <property type="entry name" value="HET DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_1G17550)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06985">
        <property type="entry name" value="HET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Heterokaryon incompatibility" evidence="1">
        <location>
            <begin position="214"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="669" mass="74820" checksum="8FDB7D5EEAC20FB9" modified="2010-06-15" version="1">MLPKYLWMWVAAECVPGWVIDSEHLPRVQECLSVVNSTANRLAKLSVGAVSEEAWNAGFGYPPGHAVLLSVILLGELLDNALAGVVFSLPKMKMLSWEYSLFGKYLLQKAGWCLGELDMLGITEPAILFYVGSFDRVALKKDHSRCSDRVCLANQIDERVYQTKHVMETCTCEHIIVEEEGNRPVTEVLHKGDIPTISFDGEKVFVHSSDSAPYVAMSHVWSDGLGNPHTNSLPKCQLERIQGLVNALHPDRDPASPVPFWLDTVCVPLHAETRKTAIRRMAKTYDSAAKVLVLDVSLSGTSAGVPADELLMRIRCTPWTQRLWTLQEGMLAEELYFQFRDKAIAAESLPDNWYYENSPVKLCGKHFGRPRADNLPLETRVFRALASDADEFVKDEMAMESALETLSRHPDCPDILDHTLLYRLDTNHSASFHPVYFAGWTSFQKLRYRFGVGHSALPSVAGALRGRLSSKMDDETICVATLLDIDPLHILSAKSQIARMKVLIDSVDNFPPSLIFTNVPRMDLDGYRWAPESFMDKNANYAGLLRAGETGRRTEDGLLVSNYFSYAFPKDWTFPDAGSFVVKGWESYFRITHAHALTVEVARPAVILEQPPATVSRGIVVDMWREDDGVVKFGRHVGHVSIEALGGEWAGEVVCEVVKWPISYKWCVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIK0</accession>
    <name>D5GIK0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008532001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430327">
        <property type="protein sequence ID" value="CAZ84343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840152.1">
        <property type="nucleotide sequence ID" value="XM_002840106.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIK0"/>
    <dbReference type="GeneID" id="9183277"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008532001"/>
    <dbReference type="HOGENOM" id="CLU_723992_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIK0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="145"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="287"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="159"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="309"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="329"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="382" mass="42146" checksum="DB80569EE1EC5115" modified="2010-06-15" version="1">MSFGRRLRKAVSKLICRRGSKPRTDTTLAMIHPSQSFQSRPYETPVGVGASERAQPPPVAQSRSQLEPSQAHRLSATGPDPEPENRNHGESSREEEHVSEENRRQSNLWRSPTNISTSSSDYSNHSISHSLSPATPVPWLGRTLMDSHFVNTPPPTPPGSDSTDGSDPASGEGVKERGHAPLIPQMSFIQRNSSAPDELRTEEALSQLRITDDGRGIVYGQFTPQDSPITGQTPHDIPTNTTFETSYPKPTTLTMVHPQEIEQTTDLLDHEVHTSTAHTRIQPVVAQEHLPTERRPETPTGEVAELTDEETRQDDWEEVWRSEWASNKEGSRKLRTYDKGNGTESKGTDDAPALLVRPKEPGLMEVKGSTAGPSRAPMTPSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJ09</accession>
    <name>D5GJ09_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00008769001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic/archaeal RNase P protein component 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840311.1">
        <property type="nucleotide sequence ID" value="XM_002840265.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJ09"/>
    <dbReference type="STRING" id="656061.D5GJ09"/>
    <dbReference type="GeneID" id="9181843"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008769001"/>
    <dbReference type="eggNOG" id="KOG4046">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1397253_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJ09"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030677">
        <property type="term" value="C:ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033204">
        <property type="term" value="F:ribonuclease P RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.210">
        <property type="entry name" value="Ribonuclease P/MRP, subunit p29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00754">
        <property type="entry name" value="RNase_P_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016848">
        <property type="entry name" value="RNase_P/MRP_Rpp29-subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036980">
        <property type="entry name" value="RNase_P/MRP_Rpp29_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023538">
        <property type="entry name" value="RNP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023534">
        <property type="entry name" value="Rof/RNase_P-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002730">
        <property type="entry name" value="Rpp29/RNP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13348:SF0">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13348">
        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01868">
        <property type="entry name" value="RNase_P-MRP_p29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00538">
        <property type="entry name" value="POP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101744">
        <property type="entry name" value="Rof/RNase P subunit-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="28"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="34"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ84502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="195" mass="20886" checksum="D3E63D48EC5FEB89" modified="2010-06-15" version="1">MSLNTLLSSAYSPAITSEVHSSITYKPLHLHPNTLPPPPPAPPSKPLTATQKRAQKIHQIPKSVPPTTLRALHALWSDYATDLLRSTSSSAAAAQLMASAEFVGARVEVVRSACPSLVGIVGVCVIERRGVVYIACEESGRVRGAGKGVCVFRIEVGGGKEGQGRRVFEVHGRRFLVRPGERGGKKFKGRGMLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJ42</accession>
    <name>D5GJ42_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein BCP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00008831001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 3">Involved in nuclear export, actin cytoskeleton organization and vesicular transport.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the BCP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840344.1">
        <property type="nucleotide sequence ID" value="XM_002840298.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJ42"/>
    <dbReference type="FunCoup" id="D5GJ42">
        <property type="interactions" value="948"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJ42"/>
    <dbReference type="GeneID" id="9181876"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008831001"/>
    <dbReference type="eggNOG" id="KOG3034">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068770_2_0_1"/>
    <dbReference type="InParanoid" id="D5GJ42"/>
    <dbReference type="OMA" id="VKFYRKE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025602">
        <property type="entry name" value="BCP1_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13261">
        <property type="entry name" value="BRCA2 AND CDKN1A INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13261:SF0">
        <property type="entry name" value="BRCA2 AND CDKN1A-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13862">
        <property type="entry name" value="BCCIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028983">
        <property type="entry name" value="BCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="7"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR028983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ84535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="299" mass="33369" checksum="C4F6F32A61FA5649" modified="2010-06-15" version="1">MGKRKASKDTDTRDVEIGNGGEENTNSDSSSDSEEIDIVNVDFEMFDPQPDHDFHGLKTLLRQLFDADNTLFDLSALTDLILSQPLLGTTVKVDGNESDPYAFLTVVNLQEHKDKPVVQDLIKYILARSQSSPNLNKKLNELLSSSASSHTGLILTERLINVPTEIVPPMYKMLLEEINWAIEENEPYNFSNFLIISKTYTEIPSKIDALESRPQKKGKKGSSKSKNELFYFHPEDETIQRSGEHASYKYVKEGGTGTADSKRAFSDFGIMPQGHIILVGKDELPKIVEGLEKLFSPGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJ66</accession>
    <name>D5GJ66_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008862001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840368.1">
        <property type="nucleotide sequence ID" value="XM_002840322.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJ66"/>
    <dbReference type="GeneID" id="9185927"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008862001"/>
    <dbReference type="HOGENOM" id="CLU_712107_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJ66"/>
    <dbReference type="OMA" id="FLHATHN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="346"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="388" mass="43165" checksum="A83DB3CE9019FDEF" modified="2010-06-15" version="1">MIWLSGFQLAANGLLQIYGNGWENPIFIPLGFAEFVPEREYSPDDPEYSMYMKFRKDPQRALEGKRKVVDALIDYLGKRYGKKFGGNIEPEIATVYYHYPLRPREYKRKGLKISPPPPLPDDTPPPLRISNLTYEWTTRPIPTATMHRLITAFYPTWMFSSVTTSLQHTYSFLHATHNSTQTSPASSPIDLGKRAISHGIQSGLVHLRRTYRRPPAPQGHVVLDGLVQVAGDKLRVAMDVTVSFHPEDLNSIIFHKIDVRYVARNDKRQKVIAPPVASPGQAPPHQVTTIRVVVVKSATEQPKTEIIEAAAALQRSNVDKARSFIDKQDKERKKVKKEAVEVGVEKEVTAVTPGAEGAAVGGPTPPVSPEEEKEGSKRTGDDAASSKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJD0</accession>
    <name>D5GJD0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00008943001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430330">
        <property type="protein sequence ID" value="CAZ84623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840432.1">
        <property type="nucleotide sequence ID" value="XM_002840386.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJD0"/>
    <dbReference type="STRING" id="656061.D5GJD0"/>
    <dbReference type="GeneID" id="9185991"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008943001"/>
    <dbReference type="eggNOG" id="ENOG502SANW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_821808_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJD0"/>
    <dbReference type="OMA" id="CQMENDD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="247"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="55"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="279"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="299"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ84623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="338" mass="37898" checksum="DC8150204601A8C0" modified="2010-06-15" version="1">MRELAEIRDKEEKAYASEVAERETLVKTMKNWGDKASLLEKEIHKIEEGEEGKEARALREAKSEVEREMQELRTQLSKLEDHRAALRYKLGELESTVSSKTSSYQSSLVTVKQRTSNFLATYRFPSPATAIESWTLEREALLAKKDQAKVEGEALLEGMVLWDETLSLVYSFEDKLGLLISGRRSGDINRDIMQGLEGVVEALEQKLELAENRGWKLLVCCIGAELEAFREAKEVMKRSLGITSASRAEASKLASMEPNKDERKGGDQNQDGEDLITGLKERGRTRDRSLIGEVGGDGEGDKAEQKESDESEGKSEGGVRDLRSSIFTLRKDYSPLDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJD9</accession>
    <name>D5GJD9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00008954001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430330">
        <property type="protein sequence ID" value="CAZ84632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840441.1">
        <property type="nucleotide sequence ID" value="XM_002840395.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJD9"/>
    <dbReference type="STRING" id="656061.D5GJD9"/>
    <dbReference type="GeneID" id="9182633"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008954001"/>
    <dbReference type="eggNOG" id="KOG0255">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008455_0_4_1"/>
    <dbReference type="InParanoid" id="D5GJD9"/>
    <dbReference type="OMA" id="MTWIACA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF134">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="173"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="203"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="286"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="325"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="364"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="391"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="427"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="457"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ84632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="496" mass="54576" checksum="AC5FEF592509C15A" modified="2010-06-15" version="1">MPRPRAPQEGPEIIKHVLPTVFDLEKITIVDDSPAPPYAEKLPSGFSYLEWNTPPEALFADPEQLPSTVTCYTDPFTWPRAQKNAILILTSLSTAMGAYSAGAYTSGIDEMMEEWHVSRIVMLLGITTFTIGFAVPPLVLGPLSEVFTFGLFNVCHMLSALSHSATGMIVTRFFLGVGGSTFGAMIGGIISDIFHAPDRGLPMALFSMAGMAFTGLGPLVTFPILKETRGSVLLSRKAKALNKFLDEREDRGVRWKVKADEERESISLMVRVALTRPFRFLVTESVVFWFSCWLTFAWGILYMFLEAIPLVFVENHGFTVAQVGYVFAAMPIGSVAALCCNSLVEMSTEKYLSSSTPNPEARLYSACTIGCLLPIGLFWFGWTSFPSQHWILPTVAVGCVTMGIYSIYLAAFNYLADTYHRYASSALAAQSFCRNFFAGGFPLIADMMYHRLGFAGASSLLGCVGALLTIVPWVLMFYGTNIRARSKFAKVFQTVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJE3</accession>
    <name>D5GJE3_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mannose-P-dolichol utilization defect 1 protein homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00008959001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the MPDU1 (TC 2.A.43.3) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430330">
        <property type="protein sequence ID" value="CAZ84636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840445.1">
        <property type="nucleotide sequence ID" value="XM_002840399.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJE3"/>
    <dbReference type="STRING" id="656061.D5GJE3"/>
    <dbReference type="GeneID" id="9182637"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008959001"/>
    <dbReference type="eggNOG" id="KOG3211">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053568_0_1_1"/>
    <dbReference type="InParanoid" id="D5GJE3"/>
    <dbReference type="OMA" id="LQVLYYW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016817">
        <property type="entry name" value="MannP-dilichol_defect-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12226">
        <property type="entry name" value="MANNOSE-P-DOLICHOL UTILIZATION DEFECT 1 LEC35 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12226:SF2">
        <property type="entry name" value="MANNOSE-P-DOLICHOL UTILIZATION DEFECT 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF023381">
        <property type="entry name" value="MannP-dilichol_defect-1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00679">
        <property type="entry name" value="CTNS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="150"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="209"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="235"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="261"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR023381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ84636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="292" mass="31407" checksum="C6383CE9FFB7BA3E" modified="2010-06-15" version="1">MDSILQSLEGPVFSTIRPYITPYTSTLPPSIQKPLLNLLTPICYNNLIRDFDPASNPQCVQYAISKAIGLGIVTLSTIVKVPQLIKLLSSQSSKGLSFLSYLLETTAFLCTLAYNFRSGNPFSTYGEIAMIAVQNVLISTLILQYSGKGGWGAVWVAALAAAGYALFNEGIVSPDIMVYLQAATIPLGLASKVPQIIEVARQKSTGQLSAFAIFNYLFGSLARVFTTLSEVNDPLILWGFLGGAALNTVLAAQMVYYWNSGKKGGKSPNTPSKKFYAGGKSPKPKGDPKKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJU0</accession>
    <name>D5GJU0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00009190001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430333">
        <property type="protein sequence ID" value="CAZ84783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840592.1">
        <property type="nucleotide sequence ID" value="XM_002840546.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJU0"/>
    <dbReference type="GeneID" id="9186819"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009190001"/>
    <dbReference type="HOGENOM" id="CLU_3368798_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJU0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ84783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="35" mass="3830" checksum="2A00C92620E914C1" modified="2010-06-15" version="1">MVSLRFLLCGSRVLDLGHRNGGCDLVTSVSYLGYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5GK35</accession>
    <name>D5GK35_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00009344001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430335">
        <property type="protein sequence ID" value="CAZ84878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840687.1">
        <property type="nucleotide sequence ID" value="XM_002840641.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GK35"/>
    <dbReference type="FunCoup" id="D5GK35">
        <property type="interactions" value="34"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GK35"/>
    <dbReference type="GeneID" id="9186915"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009344001"/>
    <dbReference type="eggNOG" id="KOG1564">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013059_1_1_1"/>
    <dbReference type="InParanoid" id="D5GK35"/>
    <dbReference type="OMA" id="IACKDLE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033065">
        <property type="term" value="C:Rad51C-XRCC3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005657">
        <property type="term" value="C:replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000400">
        <property type="term" value="F:four-way junction DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000730">
        <property type="term" value="P:DNA recombinase assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007533">
        <property type="term" value="P:mating type switching"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071140">
        <property type="term" value="P:resolution of mitotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090656">
        <property type="term" value="P:t-circle formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000722">
        <property type="term" value="P:telomere maintenance via recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19491">
        <property type="entry name" value="XRCC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013632">
        <property type="entry name" value="Rad51_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047348">
        <property type="entry name" value="XRCC3-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46487">
        <property type="entry name" value="DNA REPAIR PROTEIN XRCC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46487:SF1">
        <property type="entry name" value="DNA REPAIR PROTEIN XRCC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08423">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecA family profile 1" evidence="8">
        <location>
            <begin position="80"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="223"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="292"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="230"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="294"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ84878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="408" mass="44194" checksum="DB9A1CC8B6FC390D" modified="2010-06-15" version="1">MSDLLTLFPTFPLTPPVSNLLNFIGDARLTTADLVALDPMSISRRLPSQLNTSLLELKSLQRTLVEHIHADIARLSSLPPPRTITTHSPTLDRLLNKIPTQAITEFAGESGSGKTQILLLLTLTVQLSPSRGGLGRPAVYISTEAPLSTLRLIQMKNYISRDLPVGEQPTTDKVFSIVCSDLETQEHIIRYQLPVLVQKFNVGLVVIDSIAANFRAEFERPMNRNKKLRSSNTSTPSSSPSSSSTGAPSAGARHGSQMAQRGKEMVRLAATLRHLAINHNLAVVVSNQVSDKFTPSASSRTPLSSSSSARDRDDDDDDIMSLDFQLKWFAGCDEGLEGGGKIPALGLVWANLLATRVVIRKSEDPKTGESRRSLRVAYSNRALAGEEVGIEVFEGGVKSVNDGGDECS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5GK39</accession>
    <name>D5GK39_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00009350001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the purine-cytosine permease (2.A.39) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430335">
        <property type="protein sequence ID" value="CAZ84882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840691.1">
        <property type="nucleotide sequence ID" value="XM_002840645.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GK39"/>
    <dbReference type="FunCoup" id="D5GK39">
        <property type="interactions" value="559"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GK39"/>
    <dbReference type="GeneID" id="9186919"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009350001"/>
    <dbReference type="eggNOG" id="KOG2466">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021555_4_0_1"/>
    <dbReference type="InParanoid" id="D5GK39"/>
    <dbReference type="OMA" id="AFRTHEW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015205">
        <property type="term" value="F:nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4160.10">
        <property type="entry name" value="Hydantoin permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001248">
        <property type="entry name" value="Pur-cyt_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045225">
        <property type="entry name" value="Uracil/uridine/allantoin_perm"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30618">
        <property type="entry name" value="NCS1 FAMILY PURINE/PYRIMIDINE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30618:SF0">
        <property type="entry name" value="PURINE-URACIL PERMEASE NCS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02133">
        <property type="entry name" value="Transp_cyt_pur"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="85"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="178"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="206"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ84882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="271" mass="30150" checksum="96A68233CBD8FF25" modified="2010-06-15" version="1">MPFSSSLTFARVVPHLRLRLPYKAGAYGTARWSNKDLDPVPVELRTWGGLDYWAYWCSNMLAPPLAATVSTVMSLGFTARETIPIVFFGFMICSVVVTLTGKMGATYHVAYPVIVRSTFGMIGSYPAIVIRAFVAMMWTAILCVQAGAFLQNCIEVIWPSFKKFPNHLSEGAGIDCRSFSAGLLFFFLYWILQTILSLMPIQKLRLLFLAKAIIVPPTFLALFLWAVIVTHGGGPLVTGKYVLTTPMNTAYSSLTGDRPYLFYGSKHARFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GL35</accession>
    <name>D5GL35_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00009949001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in nucleolar processing of pre-18S ribosomal RNA. Has a role in the nuclear export of 40S pre-ribosomal subunit to the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOP14 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430344">
        <property type="protein sequence ID" value="CAZ85228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841037.1">
        <property type="nucleotide sequence ID" value="XM_002840991.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GL35"/>
    <dbReference type="FunCoup" id="D5GL35">
        <property type="interactions" value="1079"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GL35"/>
    <dbReference type="GeneID" id="9185822"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009949001"/>
    <dbReference type="eggNOG" id="KOG2147">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008874_0_0_1"/>
    <dbReference type="InParanoid" id="D5GL35"/>
    <dbReference type="OMA" id="KSCWPSL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030692">
        <property type="term" value="C:Noc4p-Nop14p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007276">
        <property type="entry name" value="Nop14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23183">
        <property type="entry name" value="NOP14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23183:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04147">
        <property type="entry name" value="Nop14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="162"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="249"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="281"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="378"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="23"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="205"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="281"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="404"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ85228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="860" mass="97100" checksum="984CBA7B64A65E84" modified="2010-06-15" version="1">MPESQLKRLKASLRTAGITGQQKSKKAAKKQRKSTIKHDSRLDRHATLQTIREEFNPFEIKTTREKHAVIGRGKAKGAQGRPSLSKQIGEENRKRTLLVEMQKRNKAGGILDRRFGENDPTITPEEKMLQRFTKEKQKRVRGGDIFNLDDDDELTHFGQSLGGLTDDFDAQNDLMLSDDEPTRKRPLDTEGLGDEDASPGRKKSKTEVMKEVIAKSKLHKYERQKAKEDDEEEREKLDAQMNDIWALLGAKKPSATGPATGANNEPGREYDYDVAVREMAFDKRSKPSERTKTEEEKLQEESERLRKLEEVRQKRMRGEEDSGDEKEALGGDDVDLGDAAEYGLGAGVPMAPVGGNFNPDELVDGDYEASEDGYIDVDENGEANASDTEQLDDYNSDYSGVGGSDDEPEEGEDDDFLETLGSQSGRLAFTFPCPQTHGEFLEIVGDIPIDDLPTVVQRIRVLYHPKLDAENKHKLARFSEILLQHILLVANKAPSAPPFKPLETLIRHLHALAKSYPDTVSRAFREHLNIIHEQRSTGDLKAGDLILFTAISTIFPTSDHFHQVVTPAMIVICQWLGQVSPNSLSRLGVGTYLVTMCVQYQRLSKRYVPEATHFVLRALSLLAPVRQEKLSGTFPYEEPEVSLRIQKPPPRETVEARKMNFADIFGAEESGADRRDIQLSLLATLASLLESMAGLWVGKTAFTMIFEPALEIIEHLLHKSNKSAIGGTYREKLLATKSYLESRILRARKSLRPLTLHYHRPLPIKTYIPKFEEAYSLDKRSYDPNADRVALSKLKAEHKREKKGALRELRKDSRFIAREKLKEDKKTSKEYHEKMRKLTAMIQTEEGAAGNEYKKAKGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLU1</accession>
    <name>D5GLU1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00010416001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic ribosomal protein eS10 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430351">
        <property type="protein sequence ID" value="CAZ85508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841317.1">
        <property type="nucleotide sequence ID" value="XM_002841271.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLU1"/>
    <dbReference type="STRING" id="656061.D5GLU1"/>
    <dbReference type="GeneID" id="9186492"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010416001"/>
    <dbReference type="eggNOG" id="KOG3344">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089349_0_2_1"/>
    <dbReference type="InParanoid" id="D5GLU1"/>
    <dbReference type="OMA" id="HEHIFRE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000025">
        <property type="entry name" value="40S ribosomal protein S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005326">
        <property type="entry name" value="Plectin_eS10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037447">
        <property type="entry name" value="Ribosomal_eS10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12146">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12146:SF0">
        <property type="entry name" value="RIBOSOMAL PROTEIN S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03501">
        <property type="entry name" value="S10_plectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Plectin/eS10 N-terminal" evidence="7">
        <location>
            <begin position="65"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="29"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="153"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="170"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ85508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="227" mass="25817" checksum="D29825CE4E1407DC" modified="2010-06-15" version="1">MKTPTVLQSRLRHFFGRIVVTVYDTPSTLPGQNPFRTQQSQSTQRQRHSRNFPSSQPHKVAIMLIPKADRKKIHEHIFREGVLVAKKDFNLPKHTDIDTKNLYVIKACQSLTSRGYLKTQFSWQYYYYTLTPEGLDYLREWLHLPAEIVPATHVKQNRSHAPPRGMLSGDGDRRPGGGRGGERGDREGGYRRRFGDKDGGKDTQTGEFQPQFRGGFGRGRGAPPAGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMF6</accession>
    <name>D5GMF6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00010684001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430354">
        <property type="protein sequence ID" value="CAZ85699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841508.1">
        <property type="nucleotide sequence ID" value="XM_002841462.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMF6"/>
    <dbReference type="STRING" id="656061.D5GMF6"/>
    <dbReference type="GeneID" id="9182125"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010684001"/>
    <dbReference type="HOGENOM" id="CLU_195594_1_0_1"/>
    <dbReference type="InParanoid" id="D5GMF6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003883">
        <property type="term" value="F:CTP synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006221">
        <property type="term" value="P:pyrimidine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017456">
        <property type="entry name" value="CTP_synthase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06418">
        <property type="entry name" value="CTP_synth_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CTP synthase N-terminal" evidence="1">
        <location>
            <begin position="2"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="36" mass="3610" checksum="D8F2CBB154F38669" modified="2010-06-15" version="1">MKYVLVSGGVISGIGKGVIASSTGLLLKTIALRLGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>D5GN01</accession>
    <name>D5GN01_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00010994001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430361">
        <property type="protein sequence ID" value="CAZ85876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841685.1">
        <property type="nucleotide sequence ID" value="XM_002841639.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GN01"/>
    <dbReference type="STRING" id="656061.D5GN01"/>
    <dbReference type="GeneID" id="9183444"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010994001"/>
    <dbReference type="eggNOG" id="KOG2507">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_858403_0_0_1"/>
    <dbReference type="InParanoid" id="D5GN01"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01767">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46424">
        <property type="entry name" value="UBX DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46424:SF1">
        <property type="entry name" value="UBX DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00166">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBX" evidence="3">
        <location>
            <begin position="242"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="108"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="150"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ85876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="324" mass="36632" checksum="2695CA21EC74ACBA" modified="2010-06-15" version="1">MEDVYCKESIQEGSASAARDNKSLVFFVAGENCWRSMKWENENLLDGEIFPLLKYSSIVFKLKAGSAEAGFLAMFCPVEQAPYLVVVKNVSLVTKLTSDTPDSAFTQSLKSALSPTASPPPPSPPSTSVSTQGSSQNTLSSLFADRRVRLEQQQKALTAAAREKKAAEVKRRQNNLTPDRKKYVEEQTRRIAEEKAEKERILLALQHDRIEREVRELRKRQGRAAVFGTPVQAIDRTEEDKVAFNEVLVSVRLLNGRLIKARFLEGKGLAELRHWVDENRTDGAEAYTFMQPPDRKFSALDENKSLKELKFPKLMNLVLVRRWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNJ9</accession>
    <name>D5GNJ9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00011320001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430365">
        <property type="protein sequence ID" value="CAZ86092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841901.1">
        <property type="nucleotide sequence ID" value="XM_002841855.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNJ9"/>
    <dbReference type="FunCoup" id="D5GNJ9">
        <property type="interactions" value="12"/>
    </dbReference>
    <dbReference type="GeneID" id="9186168"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011320001"/>
    <dbReference type="eggNOG" id="ENOG502R7I3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014536_1_0_1"/>
    <dbReference type="InParanoid" id="D5GNJ9"/>
    <dbReference type="OMA" id="WDPYNES"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030695">
        <property type="term" value="F:GTPase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008812">
        <property type="entry name" value="Ran_GTP-bd-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31010:SF2">
        <property type="entry name" value="RAN-SPECIFIC GTPASE-ACTIVATING PROTEIN 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31010">
        <property type="entry name" value="RAN-SPECIFIC GTPASE-ACTIVATING PROTEIN 30-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05508">
        <property type="entry name" value="Ran-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="349"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="403"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="506"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="361"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="403"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="417"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="506"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="605"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="626"/>
            <end position="638"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ86092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="638" mass="71119" checksum="4C18BC377412196C" modified="2010-06-15" version="1">MDFFLAKVSQQAVSFAIRSGIIVTSQFAIKECSRYISSIKGKDRQELQDLQDRLEQKIRIISPAIDLVEIISARGNTSLESALTLTKNIRHDIQSLGVRVAGLSAQGGGRKRRRSQDEINAVVQDIKQLLRKIEDAVPFINLAITTSGVNISSNIPHNVSPSRLMQASTLLTAGDTAYSMNPFNPAQIGTTFTLSLYMLFAGHSHRQEHVSAKDLTWQEVVYKCKVKLQRVPLVYDDDQDEDGASRNTNRLRAQSKIDEYCYELCLIEDLDDGRMHEVEEGKQPPGSFDDVEKAGLRTRIPIHQISKIFYTNSGQLLGIEDSSSPILLVKRDLNAAPPRKLVDRVEQYDYGDSGSEGEYPLDMHEGDSRELEPRQELPEEYELPPHLDPEWLAFEVFTEPEPDFDDAASEYSDAEESLSVSGSSPPGSLTDPNTSIAQALSNVELSISEHPPSPTPYVSPNVKSTLSILECLLRLTILQNYQQTSHLAVHDELLNLFLSDSAWDGTRESRRQERENARRRIGFDPFGSPTKPAKEAEVGTIQLTGGSRASSHRGSRWGTPQSFGRGYRLDSSLPMTPSSDSGKPMRKLEDREQPVLANNRELWTPEQQLSSMFNSPYGTSPLSKKNSPDGKKVNTEES</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNK0</accession>
    <name>D5GNK0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00011321001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TSR2 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430365">
        <property type="protein sequence ID" value="CAZ86093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841902.1">
        <property type="nucleotide sequence ID" value="XM_002841856.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNK0"/>
    <dbReference type="FunCoup" id="D5GNK0">
        <property type="interactions" value="158"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GNK0"/>
    <dbReference type="GeneID" id="9186169"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011321001"/>
    <dbReference type="eggNOG" id="KOG4032">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074896_0_2_1"/>
    <dbReference type="InParanoid" id="D5GNK0"/>
    <dbReference type="OMA" id="QSNWGGP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019398">
        <property type="entry name" value="Pre-rRNA_process_TSR2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21250">
        <property type="entry name" value="PRE-RRNA-PROCESSING PROTEIN TSR2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10273">
        <property type="entry name" value="WGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="136"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="153"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ86093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="199" mass="22184" checksum="6790BDFCC3364398" modified="2010-06-15" version="1">METIRPAHPTTMGNVQPSSEVTAKWELGVSLILYNWDVLTEAVNNLWGGEDSSGKRDWLAGAIVEMFVGRPETDEYDIEETLLQVMGDEFSVCLEDDSSWVVARQIIVLRREIMDGNFSTVDKLHARFLARPGNPPASMFKEVVDESGSSSSEEGEGDEEMGDAPSAAPSEPRQPRQPLEPTIDEEGFELVRKRGSRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNY0</accession>
    <name>D5GNY0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00011580001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430371">
        <property type="protein sequence ID" value="CAZ86223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842032.1">
        <property type="nucleotide sequence ID" value="XM_002841986.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNY0"/>
    <dbReference type="GeneID" id="9183516"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011580001"/>
    <dbReference type="HOGENOM" id="CLU_829472_0_0_1"/>
    <dbReference type="InParanoid" id="D5GNY0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="245"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="278"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ86223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="335" mass="37973" checksum="1FA67240A3E1CAFC" modified="2010-06-15" version="1">MHLPAEKNIPCPLVKQTGISEKLTSLSNKVSAVAVDGQTRTTSANTSPRASRAQCESTTGNTSQKAWWRAKSSHAKPQQCALICFAGISPSPTAHALAPMRWELGMVRRPRHNRLHHLLQAALCHRIMDILGPELETLMSLAHYLARGTCPWIMDPPVGWSRPRELSSWAIRRLALDLGVSERNRFVGYLRACDGNGECGGFFFSFFLFLFPSHIVPFSLFFPFSCLVSPPSIIPLASFMTLRTLIYWMESLIRFFFFPLSFRTLFAGGRSRILHLSAYIGWVPPFGWCSQSYIALVFLKERIKIKNKKPVFFSFLLFPHPPRLCVIYPLTHFQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNZ3</accession>
    <name>D5GNZ3_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">GSTUM_00011594001</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="FN430371">
        <property type="protein sequence ID" value="CAZ86236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842045.1">
        <property type="nucleotide sequence ID" value="XM_002841999.1"/>
    </dbReference>
    <dbReference type="SMR" id="D5GNZ3"/>
    <dbReference type="STRING" id="656061.D5GNZ3"/>
    <dbReference type="GeneID" id="9183529"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011594001"/>
    <dbReference type="eggNOG" id="KOG0920">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001832_1_3_1"/>
    <dbReference type="InParanoid" id="D5GNZ3"/>
    <dbReference type="OMA" id="TIRSICY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd17917">
        <property type="entry name" value="DEXHc_RHA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000819">
        <property type="entry name" value="ATP dependent RNA helicase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000500">
        <property type="entry name" value="ATP-dependent RNA helicase DHX29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000002">
        <property type="entry name" value="Putative ATP-dependent RNA helicase DHX36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF145">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX57-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0934">Plastid</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="739"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="943"/>
            <end position="1125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="242"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="405"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="18"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="281"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="410"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAZ86236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1488" mass="166398" checksum="FFEB4C2CC79BC83F" modified="2010-06-15" version="1">MGGKKKNKVASIPARGFVTTSVPSKTKTPAPVPEGPSEKETDTVVASGPSEKDSGKAGEILGIPNEEEVEEHRLKALVDKLGQRARKETQRIVARVEVEKRTIRSICYPLKVNKLLEFDFVRYNSQPRTTGGVKELGVGDQILALAKEEFIQMGRTNETECKRGEALLLNAWILQRAFISMGFPKGRVEEGLQALADHGSTALDKEKGLDGALEEMFDWLALNCEEEELPGFYDEFSRGKKDVASGSSDVTPSEIKPDSGTPTPRQPFGADKRHLTGKLLRVADRSLESRDVSGEDKKVGNNESGDSEDEDVEVAPEELVPQYIKLQTQKYHLHPASTIITGKKVKGKNEIKASSIAGVSPAKAEQLGKIQNKLDELTRDPLFDLREAEDVWRVERLRLEKEEWARKQSRGGSTFKQGTKASAGYTVNSEGNGVKEETESTSPIDLPHTEEDSNIDQYFVDGLFKPTPTEELTAIQEGENENVDIRDFELVNASGGNFGKGKPRGKAGVGTPAVKKVLEELCRSRDVNSKIRFEMIPGTSISARSKLIITWSSIAESSSVDVSPITPTLGPGARPPVIISTLGLLCTTFSMASIAAMSKDQADGFIATYALFKLCGKKDEKMYLRLPSIWRELWFELLNEGQLESERQEREVLRNLEGALKIGNFGLSGPDYLAEKKPRAIKAVGPKEQGVTGGRVESGNTTSDAIKEDWFYRTSRSGYQHMLEHRRKLPMWAFKEDVLAAMEKNQVIIICGETGCGKSTQTPAFILEHELSQGKSCRIYCTEPRRISAISLARRVSEELGERKSEVGSKSSLVGYAIRLEGRMHSGTRLIYATTACFFSFLLSPELEEVTHLVLDEVHERSIDSDFLLLVLKKLLVQRKDLRVVLMSATVDADRLSAYLGDAPVMVAPGRTFPVETYYLEDAIRLTQDTLSRLNLHHINYELIVRLIDFVGSSPEYVDYSKAILIFMPGFAEIRRLNDMLIAHPTFGNNRGDGGWLIYPLHSTIASEDQEAAFSIPPSGMRKIVIATNIAETGITIPDVTCVIDTGKHKEMRFDEKRQLSRLVETFVSRANAKQRRGRAGRVQKGLCFHLFTKSQHNNWMVEQQTPEIMRLSLQDLVLRIKICRLGQVEEVLSQALDAPLPKNIRRAIDSLLEVKALTVAEELTALGRQLAKLPLDVYLGKLVLMGSIYGCLDAALTIAAILSSKSPFVTPIGHKKEAESCRLSFKRADSDLLTGWNAYSSWRRVCQRKTMMSESEFCQRNYLSSRNLLGIEELKQQLLVSVVEARFLTLKEEEKAELNRCRFSTYYRRNFFIVPESVNYSSENDSIVNSVIAASFYPKLLAKGGNGWRNILNNHRIAIHPSSVNRYAKSEWLAFHSIMQSNRSYDAHETSHVDGTAIALLCGDVDYKMHAGSMTLDGHRVRFAFEDWKSLIAFKILRSRLKEITTRSFRAPGKELTQNQRKWMQIFYRVFLRLKQIEDERVGGEAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5GP47</accession>
    <name>D5GP47_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histone acetyltransferase</fullName>
            <ecNumber evidence="2">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00011700001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-lysyl-[histone] + acetyl-CoA = N(6)-acetyl-L-lysyl-[histone] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21992"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:21993"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="2"/>
    <dbReference type="EMBL" id="FN430373">
        <property type="protein sequence ID" value="CAZ86312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842121.1">
        <property type="nucleotide sequence ID" value="XM_002842075.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GP47"/>
    <dbReference type="STRING" id="656061.D5GP47"/>
    <dbReference type="GeneID" id="9186552"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011700001"/>
    <dbReference type="eggNOG" id="KOG4534">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_511984_0_0_1"/>
    <dbReference type="InParanoid" id="D5GP47"/>
    <dbReference type="OMA" id="WINEVRY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032931">
        <property type="term" value="F:histone H3K56 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051236">
        <property type="entry name" value="HAT_RTT109-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013178">
        <property type="entry name" value="Histone_AcTrfase_Rtt109/CBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016849">
        <property type="entry name" value="Rtt109"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31571">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31571:SF2">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE RTT109"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08214">
        <property type="entry name" value="HAT_KAT11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01250">
        <property type="entry name" value="KAT11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51728">
        <property type="entry name" value="RTT109_HAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="362"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="551"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="362"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="385"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="424"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="582"/>
            <end position="593"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ86312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="610" mass="65367" checksum="E800A52A84BD2D70" modified="2010-06-15" version="1">MPKKSPPPTGGPVDDPLASLLSSLLPTTLSLKLYHISTPTSRYFPLFSSPPNQDPHRTTLESHFLVLSHENVLVFAIEVLIYTVPKQHARTFFISKADSSGYLPSNISSFATETGERKSTLRTISTAFIKYLFDAARRADPHLTATISLFARSQSQYLFPNSAENSKKHVLTDRGLIAWWGKVLDPVLQEYASSGGRGYLLVPGSDAGQTRSMIPSVAEGGVKWINGHPFKLNLDNDISVREVIPHFPDDPKARYLDELNGVAGSKDAGIGGGGKVKERAAGWRDIKTIDQFWELMSFRQECSLGRCVGFLWIVVEGVKKERKMTATGLSGEGIPEDEVVLSPISATGPTFSENILGAAATASNSSQEISPTESVGPLDFTTTSIPPPTETPTPASTPPSPASPRKRRSSSTTSFQPSKKKLRALSFSPAPSSATAPLIPPLLALDEKAYVRTIDSLLNHSDFGDIESAKRGTRKWIESVQPGVAEKGDWGVDVVGLVKVVENGRGENGNGAGAGGVAVNTLSAGLVRRKSSTPGNGVNLLDAGLVRKKSKAADEKLPGVTEEQERNGVNVLSGGLLRRGPKSREGAKGEETHVNALPTNLVRKKPKVGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GP55</accession>
    <name>D5GP55_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00011709001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430373">
        <property type="protein sequence ID" value="CAZ86320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842129.1">
        <property type="nucleotide sequence ID" value="XM_002842083.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GP55"/>
    <dbReference type="FunCoup" id="D5GP55">
        <property type="interactions" value="1021"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GP55"/>
    <dbReference type="GeneID" id="9186560"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011709001"/>
    <dbReference type="eggNOG" id="KOG2086">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029402_4_1_1"/>
    <dbReference type="InParanoid" id="D5GP55"/>
    <dbReference type="OMA" id="NKDHTDK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061025">
        <property type="term" value="P:membrane fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031468">
        <property type="term" value="P:nuclear membrane reassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14348">
        <property type="entry name" value="UBA_p47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01770">
        <property type="entry name" value="UBX_UBXN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000179">
        <property type="entry name" value="Plant UBX domain-containing protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.210:FF:000002">
        <property type="entry name" value="UBX domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.210">
        <property type="entry name" value="SEP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036241">
        <property type="entry name" value="NSFL1C_SEP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012989">
        <property type="entry name" value="SEP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23333:SF20">
        <property type="entry name" value="NSFL1 COFACTOR P47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23333">
        <property type="entry name" value="UBX DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08059">
        <property type="entry name" value="SEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14555">
        <property type="entry name" value="UBA_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00553">
        <property type="entry name" value="SEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00166">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102848">
        <property type="entry name" value="NSFL1 (p97 ATPase) cofactor p47, SEP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51399">
        <property type="entry name" value="SEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="SEP" evidence="4">
        <location>
            <begin position="192"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="UBX" evidence="3">
        <location>
            <begin position="312"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="44"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="258"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="54"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="97"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="171"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="275"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="286"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ86320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="391" mass="42102" checksum="DFD52F9D5AA0E16E" modified="2010-06-15" version="1">MSDQDSLIQNFCTVTGSDPQQARHLLEASNWDIESATAQFFEANEDDITGVSHHHLDSDSDGSEAHYDPPRQAPGGATASSSKSRFATLGDLKGKDGEEDENSDDENQDLFAGGEKSGLAVQNPGDPRNKGHIIQDILKRAAEGGPSRPQDEEAGPSRPRFSGTGRTLGSDDTESVEIPDPDAAIRRTQIPTVTRSLTFWRDGFSVEDGPLMRYDDPANQDVLRAIQNGRAPLSLMNVEPGQPADVNVFRRIDEDYVPTKKKPAPFSGTGQRLGSPTPSALSSSMAPPPANTPPAAPPASAPVPAPAVDVDDSAPHTTLQIRLSDGTRLRSRFNHSHTVGDVYGFVNGASLESRTRSYLLQTTFPTKELRDMEQTIKDAGLINAVVVQKWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPA9</accession>
    <name>D5GPA9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00011783001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the syntaxin family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430373">
        <property type="protein sequence ID" value="CAZ86374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842183.1">
        <property type="nucleotide sequence ID" value="XM_002842137.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPA9"/>
    <dbReference type="FunCoup" id="D5GPA9">
        <property type="interactions" value="596"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GPA9"/>
    <dbReference type="GeneID" id="9186614"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011783001"/>
    <dbReference type="eggNOG" id="KOG0811">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059257_2_0_1"/>
    <dbReference type="InParanoid" id="D5GPA9"/>
    <dbReference type="OMA" id="LMTYTKQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15840">
        <property type="entry name" value="SNARE_Qa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000059">
        <property type="entry name" value="Related to syntaxin 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006012">
        <property type="entry name" value="Syntaxin/epimorphin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006011">
        <property type="entry name" value="Syntaxin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF38">
        <property type="entry name" value="LD27581P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14523">
        <property type="entry name" value="Syntaxin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00914">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="258"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="4">
        <location>
            <begin position="185"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ86374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="279" mass="31094" checksum="A5574F59C1FFFD88" modified="2010-06-15" version="1">MSFDRLPSLEAQPTTSRATGYSDSPEFDRLTTQLSSQLFTLTSNISNLNRELSLVGTKRDSEALRERVKKLLNETRAGFKSVGEGVKKVQDWPDVSPSQRFVQQKLSREMSSTLADFQAIQRLSAEKTRDYVTAARQAQHLTHDEGVPTDDLAYSSKSGGQQQVQVPLVQQQFALAEQSEVEFQESLIIEREEEIRGIEQGITELNEIFRDLGTMVSQQGEMIDDIEVYVGNTATSTKAADQELTQAARYQKGARNKACCLLLILSIILTVVLLAIFLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPZ4</accession>
    <name>D5GPZ4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00012108001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430380">
        <property type="protein sequence ID" value="CAZ86587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842396.1">
        <property type="nucleotide sequence ID" value="XM_002842350.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPZ4"/>
    <dbReference type="GeneID" id="9182330"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012108001"/>
    <dbReference type="eggNOG" id="ENOG502RZIA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026017_0_0_1"/>
    <dbReference type="InParanoid" id="D5GPZ4"/>
    <dbReference type="OMA" id="MRFFMRA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="169"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ86587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="489" mass="53100" checksum="103852636EE1A746" modified="2010-06-15" version="1">MPKRRFPPVRSNKRKKELPETSEEFLDAGIGLEESGDRWRAGDKAKAGRFYFKAIESYEAALERNSRSFDATYNRQLDSIHLVTRGSPTNLGTAERKAVQSHRECLVLDPDNQDALFNTGQALCSLAEALVEYKNTPIEARSEAWKFLCEAVEIFQKCLQVQEFKRASFGPRDGSGAGDGDTDMDADEPPGGSGNPIPSQGGREDEQMSEGSGSDNAQWVIVQTPVTLSQILDTALAQLEACASLLPLCAVENLGGKSLSWAQSMGETLLSSKIIPLSQETGREQEVALAKANFEVSLGEASFRAGLTDLTGWEAAIHLAFPNSEGTTQGFQELCDRADAHIRLASTAADKTEEEEEEAVAYLAWRHYAFAAKSLSAAAKLEPGKSEIHIARGDVEILRAKLGVPAAAKSRDVLVKNAGVYYRGAKRLEGDVRVRIEAAVKEAMVTFESGDEGELLKGVEMGPIVKEVVVEAVDEGLFDAEWLTRVSLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>D5GQ42</accession>
    <name>D5GQ42_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00012190001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430382">
        <property type="protein sequence ID" value="CAZ86635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842444.1">
        <property type="nucleotide sequence ID" value="XM_002842398.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GQ42"/>
    <dbReference type="STRING" id="656061.D5GQ42"/>
    <dbReference type="GeneID" id="9185215"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012190001"/>
    <dbReference type="eggNOG" id="ENOG502SAE9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_444955_0_0_1"/>
    <dbReference type="InParanoid" id="D5GQ42"/>
    <dbReference type="OMA" id="DMARTND"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="187"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="584"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="603"/>
            <end position="614"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ86635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="614" mass="69304" checksum="A0E61A7E947CA74A" modified="2010-06-15" version="1">MSARSSKATKPAASGRITNFFKPASSQTLNAGSRNESTRQAASQEGVTRRQGSTSFEEDPTISSPTRQLLRESSVSDASTQPSSMPRDQPAKRQRVVPSSDGEESEDSDGLEDLGSMFRARKPPAQTPQKKKEPDGIVLRLRNVTIPKRPPEMPKPQTYKFSLDRIIEERAKRSSLGDDIIKARKMFEDAQKETPDGEGRTRPSEEEIGSKLGEGQASKLLGALDRKDAWRIEKSWHFFDVDKARIPKSRVAFPLENLKKGWETANLADIRVRRETLVSGFVRDMMEIGVKFSDKILEWMFIEMALESNQDLSFAYYQTLCCLSTEELLHALSSHGLVHIFQLLGGREDALNVDAPVRLGRSSRKDDVPLDYWNIQLVVKYIGKMAHGISSDEEVFKEACKLLGRLALDTLSIGHNFISTINDPDIRYNLLTTLPCVTPRAHLFRRRLALAYLFQDPTYLSKDYPDLVRLQRFARLLKTSPTFEVNNDTDYDELRATICILDIAIDDGPTSGEPNMNDLEVDVLVENFRNMFSKIRDTNAQNLSRTESKDVIERLMFRLRFGVRKREKMNLVVDTAFGTEWAGKGGKTQTKLKLKPKEKMEGDDIGDEGVEGAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5GQ61</accession>
    <name>D5GQ61_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="12">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00012213001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the XPF family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430382">
        <property type="protein sequence ID" value="CAZ86654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842463.1">
        <property type="nucleotide sequence ID" value="XM_002842417.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GQ61"/>
    <dbReference type="FunCoup" id="D5GQ61">
        <property type="interactions" value="878"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GQ61"/>
    <dbReference type="GeneID" id="9185234"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012213001"/>
    <dbReference type="eggNOG" id="KOG0442">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002265_2_0_1"/>
    <dbReference type="InParanoid" id="D5GQ61"/>
    <dbReference type="OMA" id="THILDIM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000110">
        <property type="term" value="C:nucleotide-excision repair factor 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990599">
        <property type="term" value="F:3' overhang single-stranded DNA endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000736">
        <property type="term" value="P:double-strand break repair via single-strand annealing, removal of nonhomologous ends"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007534">
        <property type="term" value="P:gene conversion at mating-type locus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901255">
        <property type="term" value="P:nucleotide-excision repair involved in interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20078">
        <property type="entry name" value="XPF_nuclease_XPF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10130:FF:000002">
        <property type="entry name" value="DNA repair endonuclease XPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006166">
        <property type="entry name" value="ERCC4_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006167">
        <property type="entry name" value="XPF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047520">
        <property type="entry name" value="XPF_nuclease"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00596">
        <property type="entry name" value="rad1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10150">
        <property type="entry name" value="DNA REPAIR ENDONUCLEASE XPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10150:SF0">
        <property type="entry name" value="DNA REPAIR ENDONUCLEASE XPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02732">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00891">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="RuvA domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ERCC4" evidence="11">
        <location>
            <begin position="680"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="478"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="488"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ86654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="902" mass="101820" checksum="BF182BDC57410292" modified="2010-06-15" version="1">MSTPTSSQPKVSLSLAYQQDLFEEVCEQDQLVILARGLGLLHIVTSLLHKYDAVGNNLVLLIGADDRENGWLGEALAEHAVRSGTPEARGLKIINTEVASVSARAEMYKQGGIFSVTSRILVTDMLTDLMDCPKISGVVVLHSERVIATSTEAFILRIYRQKNQEGFLKAFSDNPEPFTTGFAPLATMMRNLFLRSPSLWPRFHVTVARSLETKKAEVVELEVAMTDSMREIQTAVLECIETSISELRKSNSSLELDDWTVDSALHKSFDVVVRRQLDPVWHRISWKTKQIVNDLTVLRRILHCLLTYDCVTFYQYLETILATHAPPPGSTRSSQSPWLFLDAAHTIFTLAKRRVYTGKADLSKKASGKVPHGLEPVLEELPKWDMLAEVLDEIEKDGYFNPAFSQSSRSTLIMCSDESTCRQIREFLQTMHETPLEDLINQDDELEGKPNRASAAYMMRRKLRAYFAWKPDFAKLTPPPNKRRRVRGGAAAAASGPNRQNAGAVQVTEEQSAQVAALLAGVQPTEGEQNLKQEIGVDSFENMEDYYEFYEMGDLTVVHPYDGDMDERLLGELHPRYVIMYEPDPAFIRRVEVYRSSHKDREVRVYFLYYGKSVEEQRYLSAVRREKDSFTKLVHEKGSMSVTLTLDAKAIEDPQEAFLRTVNTRIAGGGRITATAVPPRVIVDIREFRSSLPSLLHGRNVEVVPCTLTVGDYILSPDICVERKSVKDLISSFKDGRLYTQCENMLLYYKNPMVLIEFDQNKSFNLEPFADLTGSISQHDLQAKLVLLTIAFPKVKLIWSSSPYQTAEIFEELKRNQGEPDPLEAIKLGLEPGEEIAGVYNQTPQEILRSIPGINPKNIKTVMSEVENLVELSNMEEKDISDLIGKEAGKQVYEFFNRSAFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>D5G489</accession>
    <name>D5G489_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00004002001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835211.1">
        <property type="nucleotide sequence ID" value="XM_002835165.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G489"/>
    <dbReference type="GeneID" id="9184957"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004002001"/>
    <dbReference type="eggNOG" id="ENOG502SBIW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1012626_0_0_1"/>
    <dbReference type="InParanoid" id="D5G489"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="114"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="161"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="114"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ79332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="275" mass="29641" checksum="F7BC4B7335355212" modified="2010-06-15" version="1">MPTSSAYYSPGTPPEDHLEHFFRELRRPSEKSLAGDDGMSIYSAAEAEAPLPPLLHPAATFAGETDIVSPLQSPRVLNTPSFGGTDVFPEMCNSPVPSELICSPLHPPTISPLTSPSLASTSQTSLSMPAPSMTHRKVPSIDPATPLAPKKLASPITFVTANSLADMIEEMNRDLEAYDATRARMIESGWSSAQEIRNVELQKEDKERNWKHRIAESKKILEAARRSEVKHSAMSSVSSIDSLGGSYPISPIIPVTILPGGDGHSMKGSERALSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5G497</accession>
    <name>D5G497_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00004012001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the annexin family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835219.1">
        <property type="nucleotide sequence ID" value="XM_002835173.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G497"/>
    <dbReference type="GeneID" id="9184965"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004012001"/>
    <dbReference type="eggNOG" id="KOG0819">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025300_4_0_1"/>
    <dbReference type="InParanoid" id="D5G497"/>
    <dbReference type="OMA" id="VRGPLMQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012506">
        <property type="term" value="C:vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.10">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001464">
        <property type="entry name" value="Annexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018502">
        <property type="entry name" value="Annexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037104">
        <property type="entry name" value="Annexin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10502">
        <property type="entry name" value="ANNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10502:SF102">
        <property type="entry name" value="ANNEXIN B11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00191">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00196">
        <property type="entry name" value="ANNEXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00335">
        <property type="entry name" value="ANX"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47874">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51897">
        <property type="entry name" value="ANNEXIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0041">Annexin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="25"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="49"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="60"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="97"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ79340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="451" mass="49665" checksum="9DD48B2D564EB19D" modified="2010-06-15" version="1">MSYPQNQNPNYGAPAGAPQQNYYAPPGPGQPYPPNPQGYPAPGVPPSPYAGQPPYHQPQGYPPPGQPQQYYPHPPPPQQGYPPHPSYNQPQYAPQGQYPPPGVHNPQYPPTPPQITYQHPPPHPQPGMPYGAPPPHHAPPPQQHVAPINATADVDAIYRACKGFGTDGRALIGVLGHRDGLTIDAIRAQYTTRIRRDLIETLEKETSGNFETALVSTALGPIESEAYFAHRAIAGFGTKESMLTEAIMGKNNQQIATLKSVYQRMYGESLENAVRGDLSGKTEQLFVMALAGNRMEDWVTVDPQVASNDVRTLYAATQGRVDDIQVCSILTRANDAYIRAYVDGYKRQYAQDFLKMIRKEFSGHMEAALLYIVRGAINKAERDAELLEDSMAGLGTKDELLISRLVRAHWDKGHFAIVKLTYAALYKRDLTKRVKGETSGDYGKMMIALAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4G4</accession>
    <name>D5G4G4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00004096001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429987">
        <property type="protein sequence ID" value="CAZ79407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842487.1">
        <property type="nucleotide sequence ID" value="XM_002842441.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4G4"/>
    <dbReference type="GeneID" id="9184072"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004096001"/>
    <dbReference type="HOGENOM" id="CLU_2470700_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4G4"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072572" evidence="3">
        <location>
            <begin position="18"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="66"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="22"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="48"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ79407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="88" mass="8000" checksum="289CE1F857D13DCD" modified="2010-06-15" version="1">MQFKILAIAALVAGASAATGVHNKTMTNGTVPTTTGGPGSGIGGGNNGSVPTGSGSPSGAPSSNGAYANAISGGLFGAAVAAGLTLVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="96" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4H6</accession>
    <name>D5G4H6_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DNA replication licensing factor MCM7</fullName>
            <ecNumber evidence="12">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">MCM7</name>
        <name evidence="15" type="ORF">GSTUM_00004110001</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the MCM family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="12"/>
    <dbReference type="EMBL" id="FN429987">
        <property type="protein sequence ID" value="CAZ79419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842499.1">
        <property type="nucleotide sequence ID" value="XM_002842453.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4H6"/>
    <dbReference type="FunCoup" id="D5G4H6">
        <property type="interactions" value="924"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G4H6"/>
    <dbReference type="GeneID" id="9184048"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004110001"/>
    <dbReference type="eggNOG" id="KOG0482">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000995_7_2_1"/>
    <dbReference type="InParanoid" id="D5G4H6"/>
    <dbReference type="OMA" id="AQHVTYV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071162">
        <property type="term" value="C:CMG complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097373">
        <property type="term" value="C:MCM core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005656">
        <property type="term" value="C:nuclear pre-replicative complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033679">
        <property type="term" value="F:3'-5' DNA/RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904931">
        <property type="term" value="F:MCM complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990518">
        <property type="term" value="F:single-stranded 3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006267">
        <property type="term" value="P:pre-replicative complex assembly involved in nuclear cell cycle DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006279">
        <property type="term" value="P:premeiotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030466">
        <property type="term" value="P:silent mating-type cassette heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17758">
        <property type="entry name" value="MCM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.10:FF:000004">
        <property type="entry name" value="DNA replication licensing factor MCM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000288">
        <property type="entry name" value="DNA replication licensing factor MCM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008050">
        <property type="entry name" value="MCM7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF26">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24901">
        <property type="entry name" value="WHD_MCM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01663">
        <property type="entry name" value="MCMPROTEIN7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 12" id="KW-0235">DNA replication</keyword>
    <keyword evidence="7 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="14">
        <location>
            <begin position="374"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="43"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="61"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU365012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAZ79419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="783" mass="87051" checksum="AB84A4D1BB6F0907" modified="2010-06-15" version="1">MSKLPVIKLSDEIDYDKQQVAFEAFLTGFKSTLSTEEAARDALSNLNMEDQDEDLTDTPRRRATRSGGRSRARRPRVAVQEPKLKYMLQLQEIANRTRDSITIELDDLRSFEDSGLVEEAVGLVKAVDKNAAHYIEIFSRAIDKVMPPETVDTTYNDDVLDLFPAMLTRRYQVYFKPLTPSGDSGNKALAVRNVRGSHLGHLITVRGIATRVSDVKPTVLVNAYTCDRCGCEIFQEVKTKSFAPLVDCPSQECKNNDARGQLFMSTRASKFLPFQEIKIQEMADQVPVGHIPRTLTVHAHGSQTRCVNPGDVVDIAGIFLPTPYTGFKAIKAGLLTDTYLEAMHFEQHKKQYDDIVIDTRTIQRIEELREQGNLYDTLAKSIAPEIFGHEDVKKCLLLLLIGGVTKEMGDGMRIRGDINVCLMGDPGVAKSQLLKYITKVAPRGIYTTGRGSSGVGLTAAVMRDPVTDEMVLEGGALVLADNGICCIDEFDKMDDSDRTAIHEVMEQQTISISKAGITTSLNARTSILAAANPLYGRYNPKFSPVENINLPAALLSRFDILFLILDTPTREGDEELGRHVTFVHMENRHPEMDFEPLTPQEMRIYIAQARTKRPVIPKEVADYIVGAYVNTRKAAKKAEKDNKSFTHASPRTLLGIIRLSQALARLRFADTVVIEDADEALRLIEVSKASLQEKSDWDEDNTVSSKIYNIIRTLAFGAGDGSGELNMRNVRERVLAKGFTDDQLADVIDEYSALDVWQVAGEGTRLVFVGAEVDAEEMDVDMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4I3</accession>
    <name>D5G4I3_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phospho-2-dehydro-3-deoxyheptonate aldolase</fullName>
            <ecNumber evidence="6">2.5.1.54</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00004117001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>D-erythrose 4-phosphate + phosphoenolpyruvate + H2O = 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14717"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16897"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="2.5.1.54"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the class-I DAHP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.54" evidence="6"/>
    <dbReference type="EMBL" id="FN429987">
        <property type="protein sequence ID" value="CAZ79426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842506.1">
        <property type="nucleotide sequence ID" value="XM_002842460.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4I3"/>
    <dbReference type="FunCoup" id="D5G4I3">
        <property type="interactions" value="151"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G4I3"/>
    <dbReference type="GeneID" id="9184055"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004117001"/>
    <dbReference type="eggNOG" id="ENOG502QPSU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030903_2_0_1"/>
    <dbReference type="InParanoid" id="D5G4I3"/>
    <dbReference type="OMA" id="DINTGLR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003849">
        <property type="term" value="F:3-deoxy-7-phosphoheptulonate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000005">
        <property type="entry name" value="Phospho-2-dehydro-3-deoxyheptonate aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006218">
        <property type="entry name" value="DAHP1/KDSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006219">
        <property type="entry name" value="DAHP_synth_1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00034">
        <property type="entry name" value="aroFGH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009395">
        <property type="entry name" value="PRK12755.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21225">
        <property type="entry name" value="PHOSPHO-2-DEHYDRO-3-DEOXYHEPTONATE ALDOLASE DAHP SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21225:SF18">
        <property type="entry name" value="PHOSPHO-2-DEHYDRO-3-DEOXYHEPTONATE ALDOLASE, PHENYLALANINE-INHIBITED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00793">
        <property type="entry name" value="DAHP_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001361">
        <property type="entry name" value="DAHP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4 6" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DAHP synthetase I/KDSA" evidence="7">
        <location>
            <begin position="65"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR001361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ79426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="390" mass="42639" checksum="D506FDFA9015993C" modified="2010-06-15" version="1">MANYYIENKNVGDRTNSEDWRIRGYNPLTPPDLLQHELQQTSNSKATVLKAREETVAILHSRDPQNRLLVVVGPCSIHDPNAALEYARRLLLLKEKYQNELLIVMRAYLEKPRTTVGWKGLINDPDIDGSFQINRGLRISRQLFIDLTELGMPLASEMLDTISPQFLADMLSVGAIGARTTESQLHRELASGLSFPVGFKNGTDGSLDIAIDAIGAAKGSHHFLSVTKPGVVAIVATMGNEDCFVILRGGKKGTNYDAASIKDSKERLRAKGTQDKLMVDCSHGNSEKNHLNQPKVAASVAEQLRNGEDCIMGVMIESHIKAGNQKVPEEGKAGLKYGVSITDACINFEDTEGVLEMLADAVKARRELLSGEPGVNVAESRPRLDPVDGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4P9</accession>
    <name>D5G4P9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Transaldolase</fullName>
            <ecNumber evidence="3 9">2.2.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00000035001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the rate-limiting step of the non-oxidative phase in the pentose phosphate pathway. Catalyzes the reversible conversion of sedheptulose-7-phosphate and D-glyceraldehyde 3-phosphate into erythrose-4-phosphate and beta-D-fructose 6-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>D-sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-erythrose 4-phosphate + beta-D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:17053"/>
            <dbReference type="ChEBI" id="CHEBI:16897"/>
            <dbReference type="ChEBI" id="CHEBI:57483"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="2.2.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Carbohydrate degradation; pentose phosphate pathway; D-glyceraldehyde 3-phosphate and beta-D-fructose 6-phosphate from D-ribose 5-phosphate and D-xylulose 5-phosphate (non-oxidative stage): step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transaldolase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.2" evidence="3 9"/>
    <dbReference type="EMBL" id="FN429990">
        <property type="protein sequence ID" value="CAZ79485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835328.1">
        <property type="nucleotide sequence ID" value="XM_002835282.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4P9"/>
    <dbReference type="FunCoup" id="D5G4P9">
        <property type="interactions" value="731"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G4P9"/>
    <dbReference type="GeneID" id="9188422"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000035001"/>
    <dbReference type="eggNOG" id="KOG2772">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047470_0_1_1"/>
    <dbReference type="InParanoid" id="D5G4P9"/>
    <dbReference type="OMA" id="THAEFLW"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00414"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004801">
        <property type="term" value="F:transaldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009052">
        <property type="term" value="P:pentose-phosphate shunt, non-oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00957">
        <property type="entry name" value="Transaldolase_TalAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000088">
        <property type="entry name" value="Transaldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00492">
        <property type="entry name" value="Transaldolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001585">
        <property type="entry name" value="TAL/FSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004730">
        <property type="entry name" value="Transaldolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018225">
        <property type="entry name" value="Transaldolase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00874">
        <property type="entry name" value="talAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10683">
        <property type="entry name" value="TRANSALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10683:SF18">
        <property type="entry name" value="TRANSALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00923">
        <property type="entry name" value="TAL_FSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01054">
        <property type="entry name" value="TRANSALDOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00958">
        <property type="entry name" value="TRANSALDOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0570">Pentose shunt</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0704">Schiff base</keyword>
    <keyword evidence="5 9" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ79485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="325" mass="35840" checksum="9074962FD35508B5" modified="2010-06-15" version="1">MTTSLDQLKATGTTVVCDSGDFATIDKYKPQDATTNPSLILAASKKPEYARLIDEAVKYGRSHGGNIDSQVDATFDRLLVEFGKEILNIVPGKVSTEVDARFSFDRQASIDKALHIIDLYKSIGIDKGRVLIKIASTWEGIQAARELESKHGIKCNLTLLFSEVQAIAAAEAGAFLISPFVGRILDWYKASTGKAYVAREDPGVKSVQNIFNYYKKYGYKTIVMGASFRNTGEITELAGCDYLTISPNLLEELYNSQAAVPKKLSSEDATALDIARKSFIDNEAEFRFYLNEDQMATEKLSDGIRKFAADAVTLKEILKAKLERA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4V8</accession>
    <name>D5G4V8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="12">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00000111001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ERT1/acuK family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429992">
        <property type="protein sequence ID" value="CAZ79551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835394.1">
        <property type="nucleotide sequence ID" value="XM_002835348.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4V8"/>
    <dbReference type="FunCoup" id="D5G4V8">
        <property type="interactions" value="188"/>
    </dbReference>
    <dbReference type="GeneID" id="9183033"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000111001"/>
    <dbReference type="eggNOG" id="ENOG502R1M5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010748_1_0_1"/>
    <dbReference type="InParanoid" id="D5G4V8"/>
    <dbReference type="OMA" id="VMTTCKL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050335">
        <property type="entry name" value="ERT1_acuK_gluconeogen_tf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056751">
        <property type="entry name" value="PAS_13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47659:SF1">
        <property type="entry name" value="TRANSCRIPTION ACTIVATOR OF GLUCONEOGENESIS ERT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47659">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24990">
        <property type="entry name" value="PAS_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="11">
        <location>
            <begin position="76"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="150"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="249"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="317"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="12"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="155"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="172"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="317"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="352"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ79551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="635" mass="68099" checksum="A2E12A981D41F0EE" modified="2010-06-15" version="1">MVKPDPDSLGETAASTTATTTTATTTPATAATATPTATPTATPTGQQSPAVPPAPSGNIASSKDPGRPRRKKARRACFACQRAHLTCGDERPCQRCIKRGLQNACHDGVRKKAKYLHDAPIDDVSEFPGGFATYPTHRVPGNINTDMLPPDGTINPGSIQSPGLQESSPTKGGNGGGRGAGGGNFPGTPLFDPSDPAFFNFDIASLNFGSQYGALEFGVLGRMSGQAADADDGSVGSYAYSPSMGTGEGYGGFSAPQDDPPHYRDSWGSGHGGRNSGAGHSVGFAGTYMIEAGPGSLTSPSPPVESPRQNYIQNTAQTTAGSSSYDTTATSGPMSSPSFYQSGGHHRPSTLQQVQSASTAQQQQQQQVPQVQAPMRRRRLPEDPSQIYSSITTPYPYTLHYHRLFHMLETRFPKPLLLRIARAMASFRPSFIACTQTLKADDLLFMEKCFQRTLWEYERFIQNCGTPTLVCRRTGEVVGVGKEFCMLTGWEKQVLLGEKANRNTNVPVNGEVVNFPPPSVDEYGHGGVGKPVFLAELLDDESVVEFYEQFARLAFGDSRGAVMTTCKVLKYRAEKDDSKKDGVREARGMDGLGAKDGKVECAFCWTIKRDVFDIPMLIVANFLPILPRPAPEGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5G579</accession>
    <name>D5G579_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 12">Adenylosuccinate lyase</fullName>
            <shortName evidence="12">ASL</shortName>
            <ecNumber evidence="6 12">4.3.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 12">Adenylosuccinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00000346001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>(2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + fumarate</text>
            <dbReference type="Rhea" id="RHEA:23920"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:58443"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="EC" id="4.3.2.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>N(6)-(1,2-dicarboxyethyl)-AMP = fumarate + AMP</text>
            <dbReference type="Rhea" id="RHEA:16853"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="4.3.2.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 12">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer. Residues from neighboring subunits contribute catalytic and substrate-binding residues to each active site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.2" evidence="6 12"/>
    <dbReference type="EMBL" id="FN429997">
        <property type="protein sequence ID" value="CAZ79672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835515.1">
        <property type="nucleotide sequence ID" value="XM_002835469.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G579"/>
    <dbReference type="FunCoup" id="D5G579">
        <property type="interactions" value="682"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G579"/>
    <dbReference type="GeneID" id="9188091"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000346001"/>
    <dbReference type="eggNOG" id="KOG2700">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030949_1_1_1"/>
    <dbReference type="InParanoid" id="D5G579"/>
    <dbReference type="OMA" id="NTPNIGW"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00132"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00075">
        <property type="reaction ID" value="UER00336"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070626">
        <property type="term" value="F:(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido) succinate lyase (fumarate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004018">
        <property type="term" value="F:N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03302">
        <property type="entry name" value="Adenylsuccinate_lyase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.275.60:FF:000001">
        <property type="entry name" value="Adenylosuccinate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.40.30:FF:000005">
        <property type="entry name" value="Adenylosuccinate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.40.30">
        <property type="entry name" value="Fumarase/aspartase (C-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.200.10">
        <property type="entry name" value="Fumarase/aspartase (Central domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019468">
        <property type="entry name" value="AdenyloSucc_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004769">
        <property type="entry name" value="Pur_lyase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00928">
        <property type="entry name" value="purB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43172">
        <property type="entry name" value="ADENYLOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43172:SF1">
        <property type="entry name" value="ADENYLOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10397">
        <property type="entry name" value="ADSL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00998">
        <property type="entry name" value="ADSL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="L-aspartase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="8 12" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Adenylosuccinate lyase C-terminal" evidence="13">
        <location>
            <begin position="354"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00017058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ79672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="462" mass="52459" checksum="5F0A1516131B2D56" modified="2010-06-15" version="1">MAQLFSNKIRFRTWRQLWVWLAKAEKQLGLPISDEAIAQMEAHLDLTDEDFKVAAEEEKRRRHDVMAHVHTFGQVAPAAAGIIHWGATSCYCTDNADLLFFRQGLDIVLPKLANVIYKPSNFALEHKDLPALGYTHCQPAQLTTVGKRACLWIQDLLWDLRNLTRARSDLGFRGVKGTTGTQASFLAIFNGDHDRVEELDRLVTRLAGFEYAYPITSQTYSRKVDADVIYAMSPFGATCQRIGGDIRHLAATKEMEEPFEKDQIGSSAMAYKRNPMRSERMCSLGRHLANLCKDALDTYSQQWFERTLDDSAIRRIDIPHAFLTTDVLLRILDNVVSGLVVYPAVIARRIREELPFMATENFIMRMVALGASRQDTHEAIRVLSHQASAIVKQEGKDNDLIERIKKDKFFEPIWGEIDGLMDARTFVGRAPEQTMKFVEKEVKVALEPWTRALSGEVSELNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5J6</accession>
    <name>D5G5J6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00004369001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835632.1">
        <property type="nucleotide sequence ID" value="XM_002835586.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5J6"/>
    <dbReference type="STRING" id="656061.D5G5J6"/>
    <dbReference type="GeneID" id="9183775"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004369001"/>
    <dbReference type="eggNOG" id="ENOG502S0ET">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030669_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5J6"/>
    <dbReference type="OMA" id="FKAAFPW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990862">
        <property type="term" value="C:nuclear membrane complex Bqt3-Bqt4"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048315">
        <property type="term" value="P:conidium formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070197">
        <property type="term" value="P:meiotic attachment of telomere to nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044820">
        <property type="term" value="P:mitotic telomere tethering at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030435">
        <property type="term" value="P:sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037548">
        <property type="entry name" value="Bqt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036887">
        <property type="entry name" value="HTH_APSES_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018004">
        <property type="entry name" value="KilA/APSES_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003163">
        <property type="entry name" value="Tscrpt_reg_HTH_APSES-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38044">
        <property type="entry name" value="BOUQUET FORMATION PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38044:SF1">
        <property type="entry name" value="BOUQUET FORMATION PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01252">
        <property type="entry name" value="KilA-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54616">
        <property type="entry name" value="DNA-binding domain of Mlu1-box binding protein MBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51299">
        <property type="entry name" value="HTH_APSES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0183">Conidiation</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0749">Sporulation</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="345"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="HTH APSES-type" evidence="5">
        <location>
            <begin position="61"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="161"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="210"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="212"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="231"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ79789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="363" mass="40068" checksum="8126F0A0BEFDE197" modified="2010-06-15" version="1">MPSEKKIKERSLPSQRNPLLQGADIPAYNELVDMRRLGQTNLGSKGGNEAVLFSYVHLRAPLPQSLEGSEIFGSMAPESYFLMRRSQDGYVSSTGMFKASFPWAKKKEEDHERQYVREHFEKTSPDETAGNLWIHPTDALALADDYGMRLWIEALLDNDTAPAPRRRSTRSVSPSKRLGSTPRKTRAKAAKDSPLKKEVAPIVEAMEEETEVVTLTTTEKTTLTSTETAPVLTNGNGIETSLTTPAKGEPTIKLRVDQESEEKGDVTVARNSEMIAKAKEMVAEAQRLDSGSDVIPVNGVGRKRKAEDVECAEEVGAGEESGYRHVKRSKVLEEELKREKVKNKALIGLTAVLAIGAIFPYVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5K4</accession>
    <name>D5G5K4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00004383001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0057 (PMP3) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835640.1">
        <property type="nucleotide sequence ID" value="XM_002835594.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5K4"/>
    <dbReference type="STRING" id="656061.D5G5K4"/>
    <dbReference type="GeneID" id="9183783"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004383001"/>
    <dbReference type="eggNOG" id="KOG1773">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107649_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5K4"/>
    <dbReference type="OMA" id="HEPAPMT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000612">
        <property type="entry name" value="PMP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21659">
        <property type="entry name" value="HYDROPHOBIC PROTEIN RCI2 LOW TEMPERATURE AND SALT RESPONSIVE PROTEIN LTI6 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21659:SF57">
        <property type="entry name" value="PLASMA MEMBRANE PROTEOLIPID 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01679">
        <property type="entry name" value="Pmp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="89"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="92"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ79797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="194" mass="21319" checksum="FFD7B876AE36115A" modified="2010-06-15" version="1">MCSSDCFLAVLAVLFPPLPVWVKRGICSADSLISLALCCLGWFPGLIHSWYIISRYPDPWDTAYAPLAHESQGTAYYYHQVAPAQHPAHSCAHQSRPQYQQPNPQYSTFRGGPPKPAPAENRQRQYTVIQPVPASPAPAGNRQPERYTSHEQQTPPETPMNGLGGEGSSMHIPSGEPPSYASIVQGDHKIQTDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5P6</accession>
    <name>D5G5P6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00001418001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430001">
        <property type="protein sequence ID" value="CAZ79839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835682.1">
        <property type="nucleotide sequence ID" value="XM_002835636.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5P6"/>
    <dbReference type="STRING" id="656061.D5G5P6"/>
    <dbReference type="GeneID" id="9187559"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001418001"/>
    <dbReference type="eggNOG" id="ENOG502QSKG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010595_0_2_1"/>
    <dbReference type="InParanoid" id="D5G5P6"/>
    <dbReference type="OMA" id="NIMEPLN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591:SF24">
        <property type="entry name" value="2-METHOXY-6-POLYPRENYL-1,4-BENZOQUINOL METHYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13489">
        <property type="entry name" value="Methyltransf_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="361" mass="40964" checksum="1BD80C6AE67EF3E3" modified="2010-06-15" version="1">MAAEGVTGQAVRDTSVRHDYNHDDDCKRSEYELSAFDGSVFDSDAGSIASSMSSAVRSNSSILSNVGNYKFEHNRRYHSYREGNWVLPNDQQEQDRMDIFHQMALLRMDDKLHLAPLENPRRILDIGTGTGIWAIEMAFKYPNARIIGTDLSPIQVSPPANCSFEVEDADSEWVYPTNNFDMIHTRQLLGGICDWRRFFEQAYAHARPGGWLELQETPPEIHTDGPAPPADLPFLRIFSLFAEASATTSRPFVDQVSQFASHMEAVGFVDVTSESRRLPLGTWSTDPTEKEIGRYNVLNFVEGMEAYTLALFTRVLGMTAEQVRSLIDEAKKDVCNRKRRWYFDLVIVRGRKPYTDEVVST</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5W8</accession>
    <name>D5G5W8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00001605001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430002">
        <property type="protein sequence ID" value="CAZ79911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835754.1">
        <property type="nucleotide sequence ID" value="XM_002835708.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5W8"/>
    <dbReference type="STRING" id="656061.D5G5W8"/>
    <dbReference type="GeneID" id="9187589"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001605001"/>
    <dbReference type="eggNOG" id="ENOG502S14R">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_452122_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5W8"/>
    <dbReference type="OMA" id="PRRISMI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013907">
        <property type="entry name" value="Sds3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21964">
        <property type="entry name" value="BREAST CANCER METASTASIS-SUPPRESSOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08598">
        <property type="entry name" value="Sds3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01401">
        <property type="entry name" value="Sds3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="395"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="473"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="578"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="67"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="138"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="156"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="422"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="477"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="496"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ79911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="604" mass="66609" checksum="7E83ADA5E5A607E8" modified="2010-06-15" version="1">MPTRKEMEDHDEDQVTGPGESIPATPTTTTSSPSKKRKRETNGSPALRPVEEPKSRQSTPPLKKKNQIPQEVSENESTSANSRRGSNTEAVSNTVSNGITQDSVMANSKDVRPSKPAKVVPPKNEVPGNAGDERDPEPVAETEVDEAADATASATLDEEPCDNVDTTREDDEDTERVKRKNAAMEDLAEIERVFAQLKDRIYSERLKRVDMEMKMVQDGTHPEFVAQKTCIDQKLEEKVRLADAHYKYGMESLSIATRVSRAQVHSQYFQTVRQLREDALERCSELWYQIQRERRAGDALVSEFSYRIPDRQSARIKQRQQYNWEVALLSGIAKHIGFPAAPDVVGASEEEIVDDLESMGIATRVPARASVTVPPTGSLRERGLDDFYERSLHTNWSHTGMQPSPPPPAPQPTQQAPGYPHVHIHHHHHHNRRHTGPNQPDLARRGPSAQGHNPGHQPSAVSLSSLLQNDVIKMEGPSTHPPSTSSLSATPHLPPRTSQLYSSQSAQHSLPPSTSLQQHVNPYVRPGNADSHQAPPPAPQRFDIGRGSTPGDVRIYGTMGGRENEAPDGGLRRLKEENDNAMGVGPPALGASAPLRYGPRMSYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6H0</accession>
    <name>D5G6H0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">GTP-binding protein RHO3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00004480001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430009">
        <property type="protein sequence ID" value="CAZ80113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835956.1">
        <property type="nucleotide sequence ID" value="XM_002835910.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6H0"/>
    <dbReference type="FunCoup" id="D5G6H0">
        <property type="interactions" value="93"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G6H0"/>
    <dbReference type="GeneID" id="9181198"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004480001"/>
    <dbReference type="eggNOG" id="KOG0393">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_21_2_1"/>
    <dbReference type="InParanoid" id="D5G6H0"/>
    <dbReference type="OMA" id="THTIMLC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005933">
        <property type="term" value="C:cellular bud"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030950">
        <property type="term" value="P:establishment or maintenance of actin cytoskeleton polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045921">
        <property type="term" value="P:positive regulation of exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090338">
        <property type="term" value="P:positive regulation of formin-nucleated actin cable assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010590">
        <property type="term" value="P:regulation of septum digestion after cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000780">
        <property type="entry name" value="Rho GTPase Rho3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00067968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ80113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="203" mass="22362" checksum="497AE5E1FAC43EFD" modified="2010-06-15" version="1">MPLCGSNKTTQRKIVLLGDGACGKTSLLNVFTRGYFPQVYEPTVFENYVHDIYVDNTHIELSLWDTAGQEEFDRLRSLSYSDTHTIMLCFSVDSRDSLENVESKWVGEIADNCQGVKLVLVALKCDLREAGDGDGPEKTVPGGFIQYPEGLAVAERIKALRYLECSAKMNRGVNEAFTEAARVALSANAKGSKESGHRACVIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6J8</accession>
    <name>D5G6J8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00004512001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430009">
        <property type="protein sequence ID" value="CAZ80141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835984.1">
        <property type="nucleotide sequence ID" value="XM_002835938.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6J8"/>
    <dbReference type="STRING" id="656061.D5G6J8"/>
    <dbReference type="GeneID" id="9181226"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004512001"/>
    <dbReference type="eggNOG" id="ENOG502S1BF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087364_0_0_1"/>
    <dbReference type="InParanoid" id="D5G6J8"/>
    <dbReference type="OMA" id="TVPWFNF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053229">
        <property type="entry name" value="NADH-Q_oxidrdct_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024549">
        <property type="entry name" value="NADH-UbQ_OxRdtase_su21_C_fun"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019721">
        <property type="entry name" value="NADH-UbQ_OxRdtase_su21_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34062:SF1">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 21KDA SUBUNIT N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34062">
        <property type="entry name" value="OXIDOREDUCTASE 21 KDA SUBUNIT, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G04750)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10785">
        <property type="entry name" value="NADH-u_ox-rdase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12853">
        <property type="entry name" value="NADH_u_ox_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="NADH-ubiquinone oxidoreductase 21kDa subunit N-terminal" evidence="2">
        <location>
            <begin position="10"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="NADH-ubiquinone oxidoreductase 21kDa subunit C-terminal fungi" evidence="3">
        <location>
            <begin position="105"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ80141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="181" mass="20729" checksum="8B2F9E06EC13DA7F" modified="2010-06-15" version="1">MDVVPPKTVHTDFPVIDTDPHFFRVLRYARPSDYAVGAGTAVAGPILFLAMDRAHPSLLPRAAMAQSLRLVAAIGVTAGFLRYYKRSSLRFWGWSENEREVEMDMREMVQKVKNKEPLYGVSMLDAHMQGVAARNSRYSQIFFHVLPWFNLVNHNQHGVDTTKYFRAAEEELEMERLARGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>D5G732</accession>
    <name>D5G732_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00002320001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430021">
        <property type="protein sequence ID" value="CAZ80325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836134.1">
        <property type="nucleotide sequence ID" value="XM_002836088.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G732"/>
    <dbReference type="GeneID" id="9182516"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002320001"/>
    <dbReference type="eggNOG" id="ENOG502SBFH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092474_2_0_1"/>
    <dbReference type="InParanoid" id="D5G732"/>
    <dbReference type="OMA" id="DVKVYRV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056539">
        <property type="entry name" value="NuiA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42093">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42093:SF1">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23151">
        <property type="entry name" value="NuiA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="13"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="163" mass="17520" checksum="6174A1FD7800C115" modified="2010-06-15" version="1">MSNEDAEYAAFLEKSNKDYSKPSGGVGASTKASEEVVKTSVPEEVHPAINALRGRTYTSDADEEFQPVSLNYEGDGLPSEEGFAKVLHLPSDSEIKVMDLGYWDVHNDYEDVRDAVVAIAGGNASLVKVYRVTGSGARAEYYVLGLAKEKGVLEGVKVLAVES</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5G737</accession>
    <name>D5G737_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00002325001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430021">
        <property type="protein sequence ID" value="CAZ80330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836139.1">
        <property type="nucleotide sequence ID" value="XM_002836093.1"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G737"/>
    <dbReference type="GeneID" id="9184244"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002325001"/>
    <dbReference type="eggNOG" id="KOG0941">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_246644_0_0_1"/>
    <dbReference type="InParanoid" id="D5G737"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000004">
        <property type="entry name" value="probable E3 ubiquitin-protein ligase HERC4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037473">
        <property type="entry name" value="Lcp-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018713">
        <property type="entry name" value="MPAB/Lcp_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37539:SF1">
        <property type="entry name" value="ER-BOUND OXYGENASE MPAB_MPAB'_RUBBER OXYGENASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37539">
        <property type="entry name" value="SECRETED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09995">
        <property type="entry name" value="MPAB_Lcp_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="446"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="domain" description="HECT" evidence="7">
        <location>
            <begin position="1199"/>
            <end position="1545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="76"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="491"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="685"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="882"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="7"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="491"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="525"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="685"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="709"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="3">
        <location>
            <position position="1513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ80330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1545" mass="170601" checksum="AB0D1CE9F9CE1AE1" modified="2010-06-15" version="1">MSPPLLPSSHSLSSHPRIPSSAIPPHSRKTTISAWGHKYVPTPLHPAAQVLTTHQESWDTLADEALDLLTLLRIPSPPASPTSPQKDLYTTPKEHHDTHPTLSTLWQRVDTVPAWVDWEQISRGQDVFYRYSGAMLMGLCYMSLLGGMGASRIAEVLYRTGGFSTGTARRRMFETMQHILQCTKSLESIKPGGAGQISSIKVRLLHAAVRKRILEIERRNPGYYSVKEFGVPVNDLDSIGTVLSFSANMVWGALPKQGLFLSSREVEDYIALWRLIAHYVGCPTHPFSTSARARAYYDVLITTQISPTPTSALLATNILTSLACQPPTYPTRTYLLASTRWLNPPCLCDALGLPPVQLSSKILVLAQCILNCYLGYTARFVPWFDKRHGAVARETLWKVIVLGEIGLGGETGFEFSYVPMLGKGTGAGEAVDTGERGTMGRRSVKVLGIFLLVVGVLGVVLGWCLVRGAQALWRMTILACLNTHDDNSMPSWPSRLFSTNNDSDGMMKHGRSRSQQTSASAGWTGSPGRGGGGSASGRGITEGGGGGGGSRSSNAQFATGHCMCCDCTVRWPSNLNVFRCTVCMTINDLKPVPSGPPGTPDTPLKEVPPVSLAGTKAIIDTCVEEYLDTRKWIKQMPAYAPPPPPVGGGGEGSVPGDIPGTPNAFPVFDSRRPLVSFLSRSEGAVMPSTSAPSAMPSVGDAPGSLPGKSGKEKGEGDAKDSGRELAKNMFKRLEDYLFLAFNDWECLNGSFVKERKDSGGRNKTEGAEIGMSELGSRSRGRREDIAFVTENIGEKEATLLMGRGRVADARGYNKSERADRSRADAKFSIRSPGIDWDAVNEFYDMITNAGGDYALERALHRSNSGDKEGDSKELSESLLSRRLGWEDEIMEISAEIEEARDNIARILLKATESVLKRPGRPLKKPEEVRFLLVILANPLLYPSFSRASTRYPSNSSSHSGVGGGPGHNPGIVKRILGLLSNLPNECHHYLVSWFTRMPETQFRRLIELVGSFITYRLTRQEGRKRSHAGGSAASAGGRLSYNDDWQIRAAARVMSLFFSANNNTAGRRGLQGSSYDTADDNLNYGQIARREARRRGQIIPTSEFYNMLLDYQDLIADFDVWESRASRFCFCQYPFLLSMGAKISILEHDAKRQMEVQARHAFFNSIASRRTINQYLVLKVRRECLVEDSLKGISEGVGSLDDIKKGLRIEFIGEDGVDAGGLKKEWFLLVARDVFDPSYGMFVYDDDSQYCYFNPNSLESSEEFFLVGVLFGLAIYNSTILDVALPPYIFKKLLHFTVPHSLAVSSIRPPLHHTLEDLAVFRPSLAHGLRQLLEFEGDVESTFCRDFVAETERFGQVIRVPLCPNGENRPVTSSNRREFVDLYLNYLLNSSVAKQFEPFKRGFYTVCGGNALALFRPEEIELLIRGSDEALDVSALKAVAIYDGWGGGNPAENDPIVKWFWNFFEKITPKEQRMLLSFITGSDRIPAMGATNLIIKVVCLGQDSNRFPVARTCFNQICLWRYKRREKLEALLWRAVTESEGFGLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7G1</accession>
    <name>D5G7G1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00002449001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430027">
        <property type="protein sequence ID" value="CAZ80454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836263.1">
        <property type="nucleotide sequence ID" value="XM_002836217.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7G1"/>
    <dbReference type="STRING" id="656061.D5G7G1"/>
    <dbReference type="GeneID" id="9188479"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002449001"/>
    <dbReference type="eggNOG" id="KOG3752">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_358313_0_0_1"/>
    <dbReference type="InParanoid" id="D5G7G1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13934">
        <property type="entry name" value="RNase_H_Dikarya_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002156">
        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00075">
        <property type="entry name" value="RNase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50879">
        <property type="entry name" value="RNASE_H_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase H type-1" evidence="3">
        <location>
            <begin position="275"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="25"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="438"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="75"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="447"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="500"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="524"/>
            <end position="539"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ80454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="782" mass="85717" checksum="B6ACDDC43987EA8B" modified="2010-06-15" version="1">MRLPSCEILRLPKCPPCRRGMHALPPLRSSSGGCGTTSSTGSTSANRQIAKMEKLEDLVFAQAAYISSTARSSETEALNERVEKLEEMLAAQAAELKAVKGKSAGVDAIERRVREMENKIQSQSGQIGKGANRVGDVETKVNAAEHRLNGFVSKLGNVEKTIPNLVTTKVADMYMSTTKQDIATLFNRIEALEALGSQGGGDGMAGTDNPIVLTPAAKSGKPSPLDIRLEKLFMDLKHHPRRFVPPSPQSTPLSEFSFASNSAPVPSQRLIRNGDPKQVLLFIDGSCISSGKLDSRPGYGVYFRREDNVYGRLEEYDGDDSASGVAEIWAAVIALQLRPWREEGFDKVVLACDAEYVVMGVCELAEKWAAKGWKAGGKPISGKDAWEKLLFELAVQEARGVRVQFWLIPRKLNLADPVARKGAGFDMAEIRSRNELLLRAPHPPPPPEREQAVLDHQEERTAPPEESEPREIQPVDAPAEKPPGLFGSLFGFLRRKPLTTGPSSKETPINSNLTPGPKATRKSSQKEEKDKTKEKKDKIATLSLIPLHPPPSHPGDWPGGTGPNQPEHVRGLNSQVTQISLSDPNIPIEQRRLAYHRSLLLNPNLDLETLQPHEITNIDFATLRKDYHISRLIRATLEDPFIKKGHKSIKADAESITSLSRRLINASPESAAVIRANKKKIKAAAALSVANILRAKALDASCRGETYALKERLTSLSSSTTKMVFHRMVWESTVPKIEEAVGALTADVRGTAGMITVLDQMIHEREAWEKKADEKETNGNGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7G8</accession>
    <name>D5G7G8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">[histone H3]-trimethyl-L-lysine(9) demethylase</fullName>
            <ecNumber evidence="2">1.14.11.66</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00002456001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>N(6),N(6),N(6)-trimethyl-L-lysyl(9)-[histone H3] + 2 2-oxoglutarate + 2 O2 = N(6)-methyl-L-lysyl(9)-[histone H3] + 2 formaldehyde + 2 succinate + 2 CO2</text>
            <dbReference type="Rhea" id="RHEA:60200"/>
            <dbReference type="Rhea" id="RHEA-COMP:15538"/>
            <dbReference type="Rhea" id="RHEA-COMP:15542"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="1.14.11.66"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the JHDM3 histone demethylase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.66" evidence="2"/>
    <dbReference type="EMBL" id="FN430027">
        <property type="protein sequence ID" value="CAZ80461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836270.1">
        <property type="nucleotide sequence ID" value="XM_002836224.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7G8"/>
    <dbReference type="STRING" id="656061.D5G7G8"/>
    <dbReference type="GeneID" id="9188486"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002456001"/>
    <dbReference type="eggNOG" id="KOG0955">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0958">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001442_4_3_1"/>
    <dbReference type="InParanoid" id="D5G7G8"/>
    <dbReference type="OMA" id="NSIWHYL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051864">
        <property type="term" value="F:histone H3K36 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140684">
        <property type="term" value="F:histone H3K9me2/H3K9me3 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15571">
        <property type="entry name" value="ePHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.650:FF:000024">
        <property type="entry name" value="Putative jumonji family transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055500">
        <property type="entry name" value="DUF7072"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034732">
        <property type="entry name" value="EPHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003349">
        <property type="entry name" value="JmjN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694:SF7">
        <property type="entry name" value="[HISTONE H3]-TRIMETHYL-L-LYSINE(9) DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23258">
        <property type="entry name" value="DUF7072"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02375">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13832">
        <property type="entry name" value="zf-HC5HC2H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00545">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51805">
        <property type="entry name" value="EPHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51183">
        <property type="entry name" value="JMJN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="JmjN" evidence="8">
        <location>
            <begin position="55"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="JmjC" evidence="9">
        <location>
            <begin position="228"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="10">
        <location>
            <begin position="500"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="145"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="443"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="791"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="943"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="998"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1092"/>
            <end position="1134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="443"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="834"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="950"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1020"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1057"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1092"/>
            <end position="1102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1103"/>
            <end position="1123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ80461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1134" mass="126486" checksum="0CE3E43EBCB515DD" modified="2010-06-15" version="1">MAAAIMDPVTTSGEGSRFEVVVEKRQLNNGEQFEVFPEEKDEEITPSYYYENMGIPVFEPTMDQFRSFKHFVNKINHYGMQSGIVKVIPPKEWTDSLASLEDKLKDIRIKNPIIQHMAGVAGEYRQENIEKQRTYNLPQWRQLCESSEHQPPAKRGERRKGQAIKEAPEREAEQAIADDEAFDGFDFRIHNADDYTPERCDELEKAYWRTLTYSNPLYGADMPGSLFDDSITSWNVAKLENLLDCLGKKLPGVNTAYLYLGMWRSTFAWHLEDVDLYSINYIHFGAPKQWYSISREDKPKFEQVMRGKFFSFSSQSALSLTYDRVAGIWPNDSKKCSQFLRHKNYLVSPSLLLGHGIKVNKLVHHQGEFVITFPFGYHSGYNLGYNCAESVNFATEAWLEYGRNAKKCECIKDSVWVDVDEIERKLRGESTEDEGDEIEYYYGDEDEDEMEANDLPTPPESVEGKPVKTKNHKKRKLEDGKERPAKVKKLRLKLSKEAAPEPCILCPNNIPYEELVATDDGQKAHRLCAIYTPETYFKWDEAQQQEVVANIPGIPRARLELRCIFCRSTKGACFQCSSKKCARAYHATCAAAAGVLVNMFDVPHYGEDGQMYPQTDIDFRCRFHRPKRPKGMDSEKLESDMVVRKFANSLIKGDVIQMQYYRGDIFGGVVIENRYSEECVVVNVLPKGTESLEVEWKWILAQDPSTVPLPLPLTEMPMYPTAQQSGVATTKAPKLVPEPDDPFCDEASGLKWGELIMAGPPVNADQAPVDLSNSIWHYLGEHSTEYIAKYTDDPEKRVPNEAASMGSTRKPRAKIIKTPAKVLKPMHHSPGPLQRSQELQSPYTPDQTRDHHSPQSHLTGGGVNISHLSQQQLWPSAYQPSVPTVSSGTSIANIVADATKAFINEHALFQTQEHAILTQILPGRETGMSASQPLTLMNDGARQHDDDFSNQESIQSSTTSNSMIDPQLMAQGTGLVATPPSQQIEAYDPVFVAQQTSALSTQTTGPAHDMTETLPRGVNSPEQVSAPTVVATPQGSSITSVPQSPPPSSALPAVDHSPNTSLPHQPLSSTPPRKLVPLADIQAALAQLARAKAQEQKAKADVEAAQAEVGAARARARQSAATAFGQTPAPPSQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7H1</accession>
    <name>D5G7H1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00002461001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430027">
        <property type="protein sequence ID" value="CAZ80464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836273.1">
        <property type="nucleotide sequence ID" value="XM_002836227.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7H1"/>
    <dbReference type="GeneID" id="9188489"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002461001"/>
    <dbReference type="HOGENOM" id="CLU_718026_0_0_1"/>
    <dbReference type="InParanoid" id="D5G7H1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="174"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="174"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="219"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="243"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="385" mass="42567" checksum="603DDD98A531E7B0" modified="2010-06-15" version="1">MQSTSRKKRYLLDELMIDIPRYDPRDDDPSEDKALRTLRLMRETHKQQRDRLLARHLRESEQWKKDAVASIDWDNTELVANVPGSPPTMLAYSVDQISTPEAEQALEIMPAEYRHTIVSIKQFDRPPNSNSNRAMLDSYKLAGMFADEGVHMRQRMVREEQDLRTLQEHEKAPYYEVLRRQDPERYNAKEGVPLPTKAGANQSHHLSLRASGARRDSGPSHTLSSSNAPGVGTATDYFGQKGGPEAVGSSGASAAKSRDPRPRPSMATPLAIQEGASATAGRQSGGFGAGPPSGHPFSDSNRLWGFASPGFALAKESPGSPMPPTSPLTLNPFDRRNSSASSQAAIINNLRNVPDDSGEAAYLKRILMTQQQQEQQHHSNQQPPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>D5G812</accession>
    <name>D5G812_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Palmitoyltransferase</fullName>
            <ecNumber evidence="10">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00002700001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="10"/>
    <dbReference type="EMBL" id="FN430038">
        <property type="protein sequence ID" value="CAZ80655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836464.1">
        <property type="nucleotide sequence ID" value="XM_002836418.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G812"/>
    <dbReference type="FunCoup" id="D5G812">
        <property type="interactions" value="24"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G812"/>
    <dbReference type="GeneID" id="9184141"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002700001"/>
    <dbReference type="eggNOG" id="KOG1312">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042181_2_1_1"/>
    <dbReference type="InParanoid" id="D5G812"/>
    <dbReference type="OMA" id="HIYLIWA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF288">
        <property type="entry name" value="PALMITOYLTRANSFERASE SWF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="217"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="11">
        <location>
            <begin position="114"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ80655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="349" mass="40843" checksum="AD5EE7E98FD770E7" modified="2010-06-15" version="1">MGTIRQVGLVILGISFFTFIALFGHVPRLRSTPIGWINRFMWSVFPRWLSYIDRRFTGGYIGPLVSGIGNYLMNEKHPVIMAFYLTLDFCPHCRYSARALRVYPYDHVNFRPGVICRTCHLRKPARSKHCSTCKHCIAKHDHHCVWINNCVGQLNIRHFIYFLGATDALLTYGVYLTFDILQNVFMLRAVIPSNVPMSSLPWSIYMHLLGIMIVEEIYIGAVFLLASLTGILSYGFTIYHLYLIWAGTTTNETIKWSDWQDDIKNGDILIAEPDESPPYAEDDEYDEDELCRHWPKRTTQLFYRRDPENVTELPRGFLWRRVESLSEMDNVYDLGWSKNFRDVFWPREL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5G868</accession>
    <name>D5G868_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00002910001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430041">
        <property type="protein sequence ID" value="CAZ80711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836520.1">
        <property type="nucleotide sequence ID" value="XM_002836474.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G868"/>
    <dbReference type="FunCoup" id="D5G868">
        <property type="interactions" value="687"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G868"/>
    <dbReference type="GeneID" id="9188313"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002910001"/>
    <dbReference type="eggNOG" id="KOG2769">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015750_2_2_1"/>
    <dbReference type="InParanoid" id="D5G868"/>
    <dbReference type="OMA" id="QAMQPKF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd24162">
        <property type="entry name" value="Prp3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013881">
        <property type="entry name" value="Pre-mRNA_splic_Prp3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027104">
        <property type="entry name" value="Prp3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010541">
        <property type="entry name" value="Prp3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14212">
        <property type="entry name" value="U4/U6-ASSOCIATED RNA SPLICING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14212:SF0">
        <property type="entry name" value="U4_U6 SMALL NUCLEAR RIBONUCLEOPROTEIN PRP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08572">
        <property type="entry name" value="PRP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06544">
        <property type="entry name" value="Prp3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pre-mRNA-splicing factor 3" evidence="7">
        <location>
            <begin position="190"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="Small nuclear ribonucleoprotein Prp3 C-terminal" evidence="6">
        <location>
            <begin position="427"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="43"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="63"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="74"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="111"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF06544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ80711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="570" mass="62713" checksum="628B2F03E68E668B" modified="2010-06-15" version="1">MASMIPSKRPPEEDPSISSKKPKQGVDQERIQKMLAEARARAAAVAAKLNNNARASAPSSASPTPPPAPPPPAAQSAADRIAAMRARVSGAVSRSSGLAAGGPPRPPLSAYRREEPDIEEASKARGGLDVGLHPALLGDGGDAGSAKRGKGGIPPKFATTMANRRAKGKSGKKQLEIQGPSADMTDPTKNPYFDPNLASKVVAPRRISKALAFNQKGKYIEQANALRRQAALEAMKKRIAEAARKVGIDEDMHADKAFAREPPPEIEWWDQGLTTAPSYETITPESLLISAADTIVTHYVQHPVLLAPPQDQLAPPMKPFPLTKKEQKKVRRQRRAEALKEKQAKVRLGLEPPPPPKIKKSNLMRVLGEEAVKDPTAVEARVNREIKERHEGHLKANEERKLTKQQRHEKLAANQEKDLARGIHVLVFRIETLANGRHRYKININAEQLALTGICILNPKFNLVIVEGGIHSITKFRKLMVNRIDWKESITARERIKGVEGDAGAEEEGQAVDMTKNMCTLVWEGEVRQKGFKRFTTERVPSDALARERLERAKMEYMWTQTKNAVAKEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5G898</accession>
    <name>D5G898_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00002941001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the SPT3 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430041">
        <property type="protein sequence ID" value="CAZ80741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836550.1">
        <property type="nucleotide sequence ID" value="XM_002836504.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G898"/>
    <dbReference type="FunCoup" id="D5G898">
        <property type="interactions" value="892"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G898"/>
    <dbReference type="GeneID" id="9188343"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002941001"/>
    <dbReference type="eggNOG" id="KOG3902">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038706_1_1_1"/>
    <dbReference type="InParanoid" id="D5G898"/>
    <dbReference type="OMA" id="QFMFNEQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22926">
        <property type="entry name" value="HFD_SPT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000023">
        <property type="entry name" value="transcription initiation protein SPT3 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003195">
        <property type="entry name" value="TFIID_TAF13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11380">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID/SUPT3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11380:SF16">
        <property type="entry name" value="TRANSCRIPTION INITIATION PROTEIN SPT3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02269">
        <property type="entry name" value="TFIID-18kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="90"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="103"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ80741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="314" mass="35653" checksum="9FFC06C8A162B4D0" modified="2010-06-15" version="1">MSNDKFKYRVEIQQMMFVSGETGEPSAETTGIIEEIVRGQVIEMLQQCTNLASRRGSRSISTDDLIFLIRHDKAKVSRLRTYLSWKDVRKTAKDNDPSGGAGAPDPADLLDDAAAGIAGGTGPVQMRQKKAKIGLPWEVASFFSEQVPEREEDEDEDEVEANAATLQRLKNADERTRGMTRDEYVHWSECRQASFTFRKGKRFREWAGFGTVTDAKPNDDIVDILGFLTFEIVQTLTEEALKVKELEDQVLLESREKSKKRKREKFLFHAEEGRTPIEPRHVEEAFRRLQVPKPKSRAMRNFSGGVVKSKLTLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8G3</accession>
    <name>D5G8G3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00002852001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430043">
        <property type="protein sequence ID" value="CAZ80806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836615.1">
        <property type="nucleotide sequence ID" value="XM_002836569.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8G3"/>
    <dbReference type="STRING" id="656061.D5G8G3"/>
    <dbReference type="GeneID" id="9186025"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002852001"/>
    <dbReference type="eggNOG" id="KOG0332">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_25_1_1"/>
    <dbReference type="InParanoid" id="D5G8G3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase C-terminal" evidence="1">
        <location>
            <begin position="12"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="165" mass="18528" checksum="7017989A904AC807" modified="2010-06-15" version="1">MVGDSQVDKYRKLIDLYHILTIGSSVIFVQKRDTAYEIQRRMEADGHKVATLHSAEEGPDRDKVVRAFRSGKSKVLIATDLLARGIDVATVSMVVNYSLPLRNNRRLDPVTYFHRIGRTGRFGRVGVAITFAHDKQSLSEVGEISNHFGVCITRVPTHDIEVCSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8U3</accession>
    <name>D5G8U3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00004843001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430053">
        <property type="protein sequence ID" value="CAZ80936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836745.1">
        <property type="nucleotide sequence ID" value="XM_002836699.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8U3"/>
    <dbReference type="FunCoup" id="D5G8U3">
        <property type="interactions" value="76"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G8U3"/>
    <dbReference type="GeneID" id="9186445"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004843001"/>
    <dbReference type="eggNOG" id="KOG2816">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017517_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8U3"/>
    <dbReference type="OMA" id="TFALICH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23507:SF40">
        <property type="entry name" value="TETRACYCLINE-EFFLUX TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23507">
        <property type="entry name" value="ZGC:174356"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="188"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="213"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="253"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="283"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="367"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="404"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="50"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ80936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="450" mass="48975" checksum="32BE7DEE7E2C00F5" modified="2010-06-15" version="1">MDSSIQDPLVGSEAEAQEILGGNGISEPPLIGGGLSAGGALYPRGLQHEGAEAGETSPLTDENRNGAPPRADEIDDWEGVPWYRKPSVFWLLPPFCLFTMAFGGAGVPRLNVILALICQQYYTDTGYTGNPILPVLMGGDNDKHCKEPEVQALVSKFTLYMSLVAGIFSALTSPRLGSLSDRHGRRLAMAFSSFGMLASEITTILAVKYPDLFSVNILLLGSLVDGLCGSFMLGMALSYSYGADCTSPARRAVAFGYFQGCLFLGIAIGPVLGGLLIERTNNILSVFYSALGAHAVFIAYVTFFMPESLSERRMVLARENHERERMERQEGAPKHWWNSLNPINFLRPLTILFPTGRGSSARLRLNLMLLAATDCVLFGVGIGSITVILIYAEAKFDWGNFESSIYVSIVNCFRVTMLFVLLPLVVTFFRRGSTAQNPLKGCDTLDIKYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>D5G918</accession>
    <name>D5G918_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 3 subunit F</fullName>
            <shortName evidence="4">eIF3f</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00004946001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the eIF-3 subunit F family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430053">
        <property type="protein sequence ID" value="CAZ81011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836820.1">
        <property type="nucleotide sequence ID" value="XM_002836774.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G918"/>
    <dbReference type="STRING" id="656061.D5G918"/>
    <dbReference type="GeneID" id="9181294"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004946001"/>
    <dbReference type="eggNOG" id="KOG2975">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027018_0_0_1"/>
    <dbReference type="InParanoid" id="D5G918"/>
    <dbReference type="OMA" id="EYFVHFH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071540">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3e"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071541">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3m"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd08064">
        <property type="entry name" value="MPN_eIF3f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000019">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 subunit F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03005">
        <property type="entry name" value="eIF3f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027531">
        <property type="entry name" value="eIF3f"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024969">
        <property type="entry name" value="EIF3F/CSN6-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10540:SF6">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10540">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01398">
        <property type="entry name" value="JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13012">
        <property type="entry name" value="MitMem_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MPN" evidence="6">
        <location>
            <begin position="31"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="309"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ81011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="332" mass="35723" checksum="A212FD8F3F49F665" modified="2010-06-15" version="1">MADKDSYIHLARPLPVVSVPPAQATTSPLSVVISAQALFSILDHSLRRNTDQERVIGTLLGVRNEDGAEVEIRNCFAVGHNESQEQVEVDMDYHKTMLGLHLKANPKEVLIGWYATSPELNTFSALIQNFYASNGDGTFPHPAVHLTMSTAPGQDISIRTYISSAVGVHPDRLADSCLFVPVPYEIKYADAERSGLELISSAKNSENRSAEVTTDIHALEKALEEVLEMLDRVSEYVARVVAGNTQASVAIGKFLMNTLSLAPKVSPADVERMFNTHIQDVLLVAYLANTIRTQIDLSNRLASANLGTATPTPAAAATNPTATGTTTTAGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5G959</accession>
    <name>D5G959_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00003155001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430055">
        <property type="protein sequence ID" value="CAZ81052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836861.1">
        <property type="nucleotide sequence ID" value="XM_002836815.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G959"/>
    <dbReference type="STRING" id="656061.D5G959"/>
    <dbReference type="GeneID" id="9184338"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003155001"/>
    <dbReference type="eggNOG" id="ENOG502R01W">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004261_0_0_1"/>
    <dbReference type="InParanoid" id="D5G959"/>
    <dbReference type="OMA" id="IMFLPHI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033596">
        <property type="term" value="C:TSC1-TSC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032007">
        <property type="term" value="P:negative regulation of TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007483">
        <property type="entry name" value="Hamartin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15154">
        <property type="entry name" value="HAMARTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15154:SF2">
        <property type="entry name" value="HAMARTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04388">
        <property type="entry name" value="Hamartin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="480"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="525"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="578"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="922"/>
            <end position="1132"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="744"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="817"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="488"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="587"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="934"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="985"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1036"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1080"/>
            <end position="1098"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1107"/>
            <end position="1132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ81052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1161" mass="130124" checksum="69D7C4D1C8607C2C" modified="2010-06-15" version="1">MSGGSLKDLSKTLNVALSAQFPLYPLPEDVLNVIHAYLNKHINSDDHDSQRLHDELLNLHESKVRNRPENHATFLACFRALRPGLVGVERLLKWWDILVRPTLDSMEQAKAVVADARAIVLSVLAYDDEDDPTGEKARGSAVFTDKLFEVFLEKTKLISSDRGAGFKEEQKQRFVSANVEVVLLAFGKRRPKVFLEKIDSFVVQKEYRLQVLGLLCSFVRLQGPHLYQILQTELLDHLLQCLENDTSTTVISLALTVLIMFMPHLCNSLAPYLPRLFVAYTRVLCWDKFGVVRLEEMKFAGHKDSYSRTNAPLPQAKGGSWGSPWQKLDSSFETATSTTPDVSDYFTFLYGLYPLNLMAFIREPYKFLERANYKDIDHLDIDEETIRQRTETYRQRHTLHPNFLNLTAETELTDQSRWMKVEPADVTAECIGLVNSSIPMGSAHDPRAKGAVPAAPAKPELPEALVPTEDIPNESLLSSEAVDDDHASNSGASAQSWRGDTPAIYPNNNCTEVLGLFSRPPVGYGAHSRDSPYTIPRDSKTPDSPTIPHTIAPCEEEERLQDMIRLQENLRANLHESMKNDSTHSLPPLPGPPPSGPPPGYGSVTASPRLDAYVHSLSQNTVPRSPALRPAASDTQGTIAFLQREVMLLKNDLNFERYLKQQHLSHIGHLQRKHIKEATAEAETQNLINTNKTLKAKLEEAKKAYTASRQGALKSKSQAKKWEAELNFKIRSLREEQMAWRSDEESTKQALQAAREEADHLKKLLAENEAEGLRSRQKLQSISSEMEEVSKLRALVDHLSSKLAQHESHSEEIEMRKQSEEAALAHAERIRMRLQSREQEAQDMKRGYENKIQELESRISSLSNSLPAAPGQAFQEMIDNALSATNARFASLRNEHNQLLSRYYNLKIKCIKLQADRELSNLPPHHSSLDHHQTSPMSQGFNDLHSSLALSDSEGSQCHSPRGGNHLYHSEGSTSPHSGSYPESAESNYSGGQNMYFSPNDRAQNTQAAPNNAMHPMSPGYTSPGPATAMGGGQFSMGFGSPEPHQHPSYQQQHQRNHPGQQPVQEHSNPPTLKRRPSIVGSTVSGADNQSMKTLGSQSERKRRRPEHWQEGKRKGGEKGGQEERQEEPVSDREWVGWYQGACLARVLVVVRGLLFLLTVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9L3</accession>
    <name>D5G9L3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00003371001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430062">
        <property type="protein sequence ID" value="CAZ81206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837015.1">
        <property type="nucleotide sequence ID" value="XM_002836969.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9L3"/>
    <dbReference type="FunCoup" id="D5G9L3">
        <property type="interactions" value="243"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G9L3"/>
    <dbReference type="GeneID" id="9185343"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003371001"/>
    <dbReference type="eggNOG" id="ENOG502S029">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070379_0_0_1"/>
    <dbReference type="InParanoid" id="D5G9L3"/>
    <dbReference type="OMA" id="SHKSEKH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007062">
        <property type="entry name" value="PPI-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12398:SF20">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY INHIBITOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12398">
        <property type="entry name" value="PROTEIN PHOSPHATASE INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04979">
        <property type="entry name" value="IPP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="264"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="233"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="271"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="306" mass="33996" checksum="CC79ECFC8485BD7E" modified="2010-06-15" version="1">MSQSHSPPTLEGPGNKRPRGILKNSASYHRSPTDKEATAAIPLSPSSENIPASVPMEASLSEKDITLQNTLQNAGRRRSSSNQAGRPASRRQSSVHELDEQASPRLKWDEANLYLTEQQKSSTMKITEPKTPYAKRYNPLEDEAELRILDAEDLVVDELDAMQAEAGEGPSSMPKRKKKEYAMPDEDIPGLELGEPEEAVPENSLKARESHSPKQVVVKDDYIGDHHHRHGDDDEGLDTPEEEEKHRKFQEMRRRHYEMKEVANLLGHPEELDEEDDDGDVQLGDAPNGVPHAPMVPVGLANGTSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9M9</accession>
    <name>D5G9M9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRM61</fullName>
            <ecNumber evidence="1">2.1.1.220</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">tRNA(m1A58)-methyltransferase subunit TRM61</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00004980001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.1.1.220" evidence="1"/>
    <dbReference type="EMBL" id="FN430064">
        <property type="protein sequence ID" value="CAZ81222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837031.1">
        <property type="nucleotide sequence ID" value="XM_002836985.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9M9"/>
    <dbReference type="STRING" id="656061.D5G9M9"/>
    <dbReference type="GeneID" id="9185359"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004980001"/>
    <dbReference type="eggNOG" id="KOG2915">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029873_1_0_1"/>
    <dbReference type="InParanoid" id="D5G9M9"/>
    <dbReference type="OMA" id="FYVGHVE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031515">
        <property type="term" value="C:tRNA (m1A) methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160107">
        <property type="term" value="F:tRNA (adenine(58)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049470">
        <property type="entry name" value="TRM61_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014816">
        <property type="entry name" value="tRNA_MeTrfase_Gcd14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12133">
        <property type="entry name" value="TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12133:SF1">
        <property type="entry name" value="TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08704">
        <property type="entry name" value="GCD14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14801">
        <property type="entry name" value="TrmI-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51620">
        <property type="entry name" value="SAM_TRM61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRM61 C-terminal" evidence="9">
        <location>
            <begin position="172"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="340"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ81222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="405" mass="44438" checksum="50ADCC3154A11667" modified="2010-06-15" version="1">MSGIFSLFPSLPVLFRPRDMRGAGGLNSIERQNMFRPRLPSILSSSIRRWSSSAQRVFCENDIALLRPVLDPTKTYLTQPLTQGKACETHRGTISHNDIIGKRPREVVTTPKGVKFRIAFPTLEEYIVSVRRLVTPVYPIDAATIVSLLDIHVTPGADPGDSSRDNNEEPVEVLEAGTGHGSLTLYLSRALSPCASALLHTMDVSAAHSSRAQKVVSNFRRGIYLRNVRFHVCDPGTWCREQLSVRDGKPFLTHAVLDLPGPQDYLFDVMRCLRPDGVVGVWCPSVSQIMVAVARVKGSGLFCERVLEFPGGTGVGAGLRAWDVRPALIRARVKKAEEVQEREGREGVLSGEELTPPSPVPERASPPSDLVPPAPVPEAFICRPTVGDRLVGGGFFAMFRRKSDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAQ3</accession>
    <name>D5GAQ3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00003733001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430085">
        <property type="protein sequence ID" value="CAZ81596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837405.1">
        <property type="nucleotide sequence ID" value="XM_002837359.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAQ3"/>
    <dbReference type="GeneID" id="9187780"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003733001"/>
    <dbReference type="eggNOG" id="ENOG502QRJE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017366_1_0_1"/>
    <dbReference type="InParanoid" id="D5GAQ3"/>
    <dbReference type="OMA" id="ANEISWE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038921">
        <property type="entry name" value="YOR389W-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35204:SF1">
        <property type="entry name" value="ENTEROTOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35204">
        <property type="entry name" value="YALI0A21131P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072628" evidence="2">
        <location>
            <begin position="21"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="273"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="279"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="298"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="315"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="377"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ81596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="731" mass="83089" checksum="38D1537968373C24" modified="2010-06-15" version="1">MLLPKPILLIPLLLSQYTAAEQFPLTKHIETNTTLEEDNSPFIFTSLQGLLSQWANVYAPVGFSVVPGVIPPQTLLYHARRDGDAPPKMEWLAFDSEMSYGIFAPRVKQLFLNTYATTRELKIVYFDGGSAVLAPGGTDAQDVILHGKVHTRGPRHLEDSIDSGDNDDTREKGPVEYDYTRMKELCEWGSGFGVEGFVRMNSGFEVFWCDFGVGLELVSRLNVTREEPEGEDTKPGHGPCHGHRKWRWWWPPSWWPPRWRHHCPGHPHCPGHGGPGHPKPPHHPKPPKHPKPPKRPEPPNLPEPPKFPEPPSRPEPPKVPEPPRHEGHPEHSPGRGHRGPPGDHPHHGLPGCGRHHPPGRDHGRGCHFRGGAGPYPHRREWPPYERGHHHPPGQGRPPRGGNGHPGKDCPHPPPKHGKPGEPPHDPYNPRFPHQVSKYANEISWEWMRSASWNFGSPEARVKLDTCRMTTFYNVSIRGLTPPGALNAKRSHSLLNITHSQAEAIKKQIEDNLSNKDLYCSGTDWQEVSNGIARRYLTRLLELRTLLNTPYLHIPPFKLYRKLRALSHALIMPFYETGSSAEDAEIRCVEEYTGRLNVTRLSISEKLLVDSVEGVLSRICKFSFGMYTEIVEHPPLTLTQDGEDESGDEYDNHHHHLFKWKEDVEALMLWLDWSGWYKCERTCSSNEICAIPLWPVFGTPWDRIPSQRFLENPRCVSIEYLEGRMAWGGYRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAQ5</accession>
    <name>D5GAQ5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00003735001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430085">
        <property type="protein sequence ID" value="CAZ81598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837407.1">
        <property type="nucleotide sequence ID" value="XM_002837361.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAQ5"/>
    <dbReference type="STRING" id="656061.D5GAQ5"/>
    <dbReference type="GeneID" id="9187765"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003735001"/>
    <dbReference type="eggNOG" id="ENOG502QV3F">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003572_1_0_1"/>
    <dbReference type="InParanoid" id="D5GAQ5"/>
    <dbReference type="OMA" id="CVEYYRD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012880">
        <property type="entry name" value="Gryzun"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021773">
        <property type="entry name" value="TPC11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14374">
        <property type="entry name" value="FOIE GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14374:SF0">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11817">
        <property type="entry name" value="Foie-gras_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07919">
        <property type="entry name" value="Gryzun"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Trafficking protein particle complex subunit 11" evidence="2">
        <location>
            <begin position="358"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="domain" description="Gryzun putative trafficking through Golgi" evidence="1">
        <location>
            <begin position="660"/>
            <end position="1261"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ81598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1265" mass="140158" checksum="03F22E6B818A4378" modified="2010-06-15" version="1">MDAYPTEFITHETPLLILSGLGEPETEAAASPLLEEGGFKISSELPPVTTSAGVQLLECFQRLDASSGVWAGRTERSTKSPAFRIRVVGRNYTLPPRKAAPPASGGSALGAEIPPTRALHSPISPLSPDTPLYPDGVISNLWLRKHQLLLPSVFVCFHELCTESNDGNIQNLRDNELIASINAAKKHFSPTTGSTISASIGGDEIRSSGYRSKFAVVLMSDKSILSSPRFDDRVSYIKRSTNLIANATFFFVPANSSAAELQQFALNFLSTLYPLAIEYYRDLSKHSRRKRNKGSVPPPTIPSSRALSQQAWNLRYEFKLGIFAEFRQEMDVAVRNYEMAYEKLLTEVFETTSSWSDRWTQARLLADITILRLIRCHLWGEQYTAAKRRWSAHVTHMTNILNRKGRGTETYGFAAWMSRWNKCLGELIQSGSLPVFNVSAPASPRMPMLRVGTEGIEPPAIHAPPEKQISVSERIGPQDLLHHAGFYYLKAAELLSVRVTRAKGISISDSADLYDLYLCPPPQEECTADHNSSQLSLLSLARREFDSRQQKRMVDSITLQIAKLKIAKAPHDESLWGEALKDLRSAARIYRKEGWWEVLEDVLWNIVQCGRNAGEGGSVVIAELELTCSSVFKERRGWRYDLSRSLEGVETVKVKPTLVVRGGDVVSFLTASYSFETPSVHAGDSIVSQLVISSDAHASAAPIVFSELKISYEGNLRTILLRHTPSSPRPDLKINKINLKGKVQEQIHSPDEPLPSPTSTKSFLVANTDLSLAPGETKVYEFSSILREAGDAKAICATFVMYTEGFELDYMVMLDTDGASGVWWAGEEGGPLKKKPLRAKEPGTLAIMPRPPKMEVTAKSAIEGELYINEIVKIELAVENGEEEDAIVDIGLRILGWPVDDDLPSVTWISDGVEEPATASLYPLGKLSPSAPPTKRTICFPSPSMPADCAIEITVHYHLISDPDTYIHKSVMTEMPIINPFSTTFDFSPRVHPDPWPDYFSLEEGTGWENGKRVLGIIQQWCLTVTLYTTGGGVVVVENWELPIHAVESGGDGSACRVITDEDSKESLLLDKVKPTPLPFTLDIQKRSLEDRSAASIDVTLVLHWRRDSLPSAPLNTTTLAVPRLPIPPSEPRVLAAVIQPSPTLTQPLPPNVITIRYTLENPTNYFLTFNVLMEANEAFAFSGPKQILVQLLPSSRAEIEYRLFVFPVPREKDVRFEGEEGGRWIKPGLRVVDRYFNKILRVSPGSEGVGIKKAGLVVWFKEAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAT3</accession>
    <name>D5GAT3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00005292001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HyuE racemase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430086">
        <property type="protein sequence ID" value="CAZ81626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837435.1">
        <property type="nucleotide sequence ID" value="XM_002837389.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAT3"/>
    <dbReference type="FunCoup" id="D5GAT3">
        <property type="interactions" value="31"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GAT3"/>
    <dbReference type="GeneID" id="9187793"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005292001"/>
    <dbReference type="eggNOG" id="ENOG502RZNN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053002_1_1_1"/>
    <dbReference type="InParanoid" id="D5GAT3"/>
    <dbReference type="OMA" id="CAGMSGM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047661">
        <property type="term" value="F:amino-acid racemase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015942">
        <property type="entry name" value="Asp/Glu/hydantoin_racemase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052186">
        <property type="entry name" value="Hydantoin_racemase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053714">
        <property type="entry name" value="Iso_Racemase_Enz_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28047">
        <property type="entry name" value="PROTEIN DCG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28047:SF5">
        <property type="entry name" value="PROTEIN DCG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01177">
        <property type="entry name" value="Asp_Glu_race"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="229" mass="24456" checksum="115AE199FFC641FA" modified="2010-06-15" version="1">MSILIVNPNSNVSMTKALEPTIENLNIPALTFTYFTAPLPSPPSIDDAESSQKSADVCFPLLFERLTSYDAFVVACYSDHPLVPLLKMHTGKPVIGIFQASVAHALHVLPREKKFGIITTGKVWEELLTEGVHRFLGGGERFAGVQSTGLAAGELHSVPEKELRRRMERATEELLESGDVGVVCLGCAGLVGLEGVVRGVAERLGLEVKVVDGVIAGMVTAGGLIGCKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5GB35</accession>
    <name>D5GB35_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00005432001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430086">
        <property type="protein sequence ID" value="CAZ81728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837537.1">
        <property type="nucleotide sequence ID" value="XM_002837491.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GB35"/>
    <dbReference type="STRING" id="656061.D5GB35"/>
    <dbReference type="GeneID" id="9187969"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005432001"/>
    <dbReference type="eggNOG" id="KOG3144">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069429_1_0_1"/>
    <dbReference type="InParanoid" id="D5GB35"/>
    <dbReference type="OMA" id="WQRWPVT"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009580">
        <property type="entry name" value="GPI_biosynthesis_protein_Pig-F"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06699">
        <property type="entry name" value="PIG-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="91"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="123"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ81728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="223" mass="23923" checksum="F841AF5EC53ADBC5" modified="2010-06-15" version="1">MPTAKTHPRRTGPTSAMRAMEPHIYTAILASTFITAFPNLVSDPASTLLWTLPGLALLQAIYQVRCLRVLKPASPSGTGRKEGMGVSSKIISSFLTTLFATTIGTAALYVLLVLFGAPVTTHVLPTLLCAMHASLLAVAPLVYKYRLEGRIWREILGGRAVIEECFAGAVGMLVGAWVGAVPIPLGTDREWQKWPVTIVTGAYIGYTVGKSLGWAYSGKRLPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>D5GB54</accession>
    <name>D5GB54_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00005457001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430086">
        <property type="protein sequence ID" value="CAZ81747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837556.1">
        <property type="nucleotide sequence ID" value="XM_002837510.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GB54"/>
    <dbReference type="STRING" id="656061.D5GB54"/>
    <dbReference type="GeneID" id="9181458"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005457001"/>
    <dbReference type="eggNOG" id="ENOG502SBZM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_924993_0_0_1"/>
    <dbReference type="InParanoid" id="D5GB54"/>
    <dbReference type="OMA" id="ARTYHAH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="48"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="301" mass="31229" checksum="D3639317B6987698" modified="2010-06-15" version="1">MSASHLTASAPSPVSITNTSPSSFTLPSNTRSIPLAHPPHQQPPSPTSSPSTSSPKHSPYHPQGARDSSRRSKSETPSPQRSVAEIPSIQTQSPPNPHQSHNHRGNRTSNSPITNQFLMLSGERPPSPLTLTSALAPPSVMPMTASGISANASVAKSTSSSRRSPAASLTLSMLPSASGSMVQPPTSAAATAATSSQMSTTSAYIHHHQAYAHSLSAQHKLQLHQKEIISQATRAAGIPPHLLSTGPMSPRLIPLGSPGPVTPLTLEDNPGGFFASHAGALEHQQLNQQLTMVASGGAMQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBH4</accession>
    <name>D5GBH4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00000500001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430095">
        <property type="protein sequence ID" value="CAZ81867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837676.1">
        <property type="nucleotide sequence ID" value="XM_002837630.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBH4"/>
    <dbReference type="GeneID" id="9184441"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000500001"/>
    <dbReference type="HOGENOM" id="CLU_434251_0_0_1"/>
    <dbReference type="InParanoid" id="D5GBH4"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="485"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="582"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="279"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="368"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="394"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="413"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="522"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="613"/>
            <end position="630"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="630" mass="69120" checksum="B8F2ADD9ECD9165C" modified="2010-06-15" version="1">MGHCGTIEATDKGEDPAEYRFVCGWCAIRICAGCRDELRFQDNRLMDLLRVIQARGGGDGLGDLNSPSELNIKIQPRVPKMGDTQLPADLSAPTLATGAEELENLSHFGPPNFSGGSTAISRPKPGGPGSETGRPRKRLTEFEEYDGEPCWPPPTRGKRDPTLSQRAELANIEEYPRQISSSHAPIRTSGVNSQRRPYQRLPGPPQWNQRGPTPQPRDPLIPRGRSPKADSPEPGRESDRESEAPVEPNDGKQTHQHVQLSHQGSPPRQPSPLVQVSPPQEPLSPLQPSPPEHEAIPSSALKVAGQQEVTLTPPPKSPRRKGSPAISMVDVPRMLRVDEPEVTQAKVTQPEARNPGARQAGVGQPEVSLEHIEKTEIKLPRAKQQPTPELGRPQVEKKQETRAKPPLPSTIPQRERTPGLDKPSRSTPSPRPEPAQVTKMGERLEESVFMGNGMPQPVEKALGSEVVKLDPVPIQMPKLAEPIPVAKQEKSVQSLTKQEKALPEPPKLVSNMIGDPTISMPVTPPKRPVYPTKPEPNIDLSYFNLKPTTTQERYKLPDSMQLPEIDFGGALDTMAITDDLLGRPARAHQKPPAAPIRLEGEEKEGSISGSMKDKKKWRLPGFGKKKVEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBR4</accession>
    <name>D5GBR4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00005537001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the oxoprolinase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ81914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837723.1">
        <property type="nucleotide sequence ID" value="XM_002837677.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5GBR4">
        <property type="interactions" value="796"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GBR4"/>
    <dbReference type="GeneID" id="9182951"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005537001"/>
    <dbReference type="eggNOG" id="KOG1939">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002157_0_0_1"/>
    <dbReference type="InParanoid" id="D5GBR4"/>
    <dbReference type="OMA" id="TDCNVML"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017168">
        <property type="term" value="F:5-oxoprolinase (ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049517">
        <property type="entry name" value="ACX-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008040">
        <property type="entry name" value="Hydant_A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002821">
        <property type="entry name" value="Hydantoinase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003692">
        <property type="entry name" value="Hydantoinase_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045079">
        <property type="entry name" value="Oxoprolinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365:SF2">
        <property type="entry name" value="5-OXOPROLINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365">
        <property type="entry name" value="5-OXOPROLINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19278">
        <property type="entry name" value="Hydant_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05378">
        <property type="entry name" value="Hydant_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01968">
        <property type="entry name" value="Hydantoinase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02538">
        <property type="entry name" value="Hydantoinase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydantoinase/oxoprolinase N-terminal" evidence="5">
        <location>
            <begin position="6"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="Hydantoinase A/oxoprolinase" evidence="3">
        <location>
            <begin position="211"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="domain" description="Acetophenone carboxylase-like C-terminal" evidence="6">
        <location>
            <begin position="537"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="domain" description="Hydantoinase B/oxoprolinase" evidence="4">
        <location>
            <begin position="735"/>
            <end position="1263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1254"/>
            <end position="1274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1262"/>
            <end position="1273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF19278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ81914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1294" mass="141050" checksum="A464BB039ED83C25" modified="2010-06-15" version="1">MSKGIRIAIDRGGTFTDCVGNPGTGKLEDDVLIKLLSVDPANYDDAPLEGIRRLMEKFTGEQIKRGDPLDTSKIESIRMGTTVATNALLERKGEKCALITTNGFRDCLVIGNQSRPKIFDLAIRRPEVLYQQVVEINERAVRILLRPTEDSIRKDLQRLYDEGFRSIAVCLMHSYTFPDHEQIVGKIAAEIGFSHISLSSQLMPMIKLVPRATSAVADAYLTPEIKRYILGFEKGFAGGLGRESVRKGGSKGARCEFMQSDGGLVDVSKFSGLRAILSGPAGGVVGYALTSYDPETKIPVIGFDMGGTSTDVSRYGSGRYEHVFETTTAGITIQSPQLDINTVAAGGGSRLFWRNGLFVVGPESAGAHPGPACYRKGGPLTVTDANLFLGRLLPEFFPKIFGKSEDEALDESTSEKLFKELTEEINKELCAEEGNKPMSPEEVAYGFIKIANESMTRPIRSLTEAKGYDTSVHRLSTFGGAGGQHAVAIAESLGIRQILIHKYSSVLSAYGMALADVVHEVQEPSSAVFGKEQKAGLVSRLEELKGKAAGVLLEQGFDEQDIVHEEYLNMRYRGTESALMVVRPQKEEFDGEEHEFGEAFVRQHEKEFGFTLPNRDIIVDDIRVRGIGKSFRGLEKTVDQQLKTIEPTPVDQSKRHSMKNVYFEGGRRETPLYRLSDLAVGEKVQGPAILADGTQTIVVTPGATALVIETHVVINLGDADEQRAGLRKISMKEVDPIMLSIFAHRFMAIAEQMGRALQKTSVSTNVKERLDYSCALFDENGGLVANAPHLPVHLGSMSTCVRTQAAIWKGRLKRGDVLVSNHPEFGGTHLPDITVITPAFSGDNIIFYVASRAHHADIGGILPGSMPPHSRELYQEGAAIKSEKLVSEGHFDEERITELLLEEPAQYPGCSGTRCLADNINDLKAQVAANQKGINLISTLIEDYGEGVVQFYMTSIQNNAETSVRNLLKSVYKRFEGKELVATEHMDDGSPICLRVDIDPQKGEAEFDFQGTGPEMYGNMNAPEAVTYSAIIYCLRCLISEDIPLNQGCLKPVHVKIPRHSFLSPSDGAAVVGGNVLTSQRITDVVLKAFQACAASQGDCNNLTFGYGGSQDGGDHVKGFGYYETIAGGSGAGPDWDGTSGVHTHMTNTRITDAEVFERRYPVILREFSIRKGSGGIGAHLGGDGVIRDIEFRIPVQVSILSERRVYHPYGMGGGGEAECGLNLWIRDKDQRPINLGGKNTAAMKPGERIVVHTPGGGGYGKPDEEKKLKNVQDPKYAWRGGSLASRLSTQETN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCS6</accession>
    <name>D5GCS6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00005999001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IWR1/SLC7A6OS family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430120">
        <property type="protein sequence ID" value="CAZ82319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838128.1">
        <property type="nucleotide sequence ID" value="XM_002838082.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCS6"/>
    <dbReference type="STRING" id="656061.D5GCS6"/>
    <dbReference type="GeneID" id="9187938"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005999001"/>
    <dbReference type="eggNOG" id="ENOG502SE90">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_520911_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCS6"/>
    <dbReference type="OMA" id="WWYENDE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040150">
        <property type="entry name" value="Iwr1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013883">
        <property type="entry name" value="TF_Iwr1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28063">
        <property type="entry name" value="RNA POLYMERASE II NUCLEAR LOCALIZATION PROTEIN IWR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28063:SF1">
        <property type="entry name" value="RNA POLYMERASE II NUCLEAR LOCALIZATION PROTEIN IWR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08574">
        <property type="entry name" value="Iwr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription factor Iwr1" evidence="3">
        <location>
            <begin position="378"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="143"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="259"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="337"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="392"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="80"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="143"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="168"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="214"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="271"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="346"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="361"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="406"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="445"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="456"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="488"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ82319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="523" mass="58253" checksum="9EF8B43D4AE16322" modified="2010-06-15" version="1">MSLPPQTIRIKRPRDEAPVQALYIDGNTRTPQKRQRTGHSPDYVFRLAHTVTSPTDPTPRPFQPRTSSPPRRYRVPQVRSTLPPSLGGSEPSPDSPSTVLPLTALTPRNVTPQPGEKLILDTATVPQSALLKAPVPLSAISSNGGSALESQTVVSPDSPPALSPVQAPERHGTPRHDTFGNESPRAGGLTPAVKRKNGEMERGQDDEEAERSGTKRKITPVRRYQLAKRLRVKHSHHPYSSATRRSAIFTRVVETHDIGTDRAEETTAVEKTPEPAVERKRPRTHPQEKKKLEEQKRKAEEEKMAEGNKVKFEDREGDNDSLIAPFHAMVLEHLNSSSNDGIRNLSSSSFSTKPKSIASKIGGGVGGGTWGGPEEEDGFVYDVYIREELPVPVDGKEGDYGVLEIEGSDDEEWWYEGDPDEGSEDDVWASEDEDSNAEDFHTNDYPEEPTYDSDDDLPRRFHPSDDDSDDDPNVFKPWVRGGQRFGDEEFDLDDDDEDEEQRFEVKQGIWDIIGGDEADDEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GD52</accession>
    <name>D5GD52_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00000977001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430140">
        <property type="protein sequence ID" value="CAZ82445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838254.1">
        <property type="nucleotide sequence ID" value="XM_002838208.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GD52"/>
    <dbReference type="GeneID" id="9183909"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000977001"/>
    <dbReference type="HOGENOM" id="CLU_1284104_0_0_1"/>
    <dbReference type="InParanoid" id="D5GD52"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ82445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="215" mass="24004" checksum="87A2BAF51EC10B6F" modified="2010-06-15" version="1">MSTSPYFDGSVVRYDVQRAGRTGVSSYTRERDFPRAATRYTRGISYNSRGSDYPYDHYDLTLAEQRELEEIRLRNEYSRAGAATVITTGSRGMRGGRTTYVTSGRAGMGMHPSSGYPTGIRTVSVAGSSPHIHRAVPRYTDQPVDYAYGGYPGDYDRYPVQGVRIPASSSGLVRRYPTTYENRRMIGAGCGYETGCREQVVVIPSHRALSPKRYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDC5</accession>
    <name>D5GDC5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00006148001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430142">
        <property type="protein sequence ID" value="CAZ82518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838327.1">
        <property type="nucleotide sequence ID" value="XM_002838281.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDC5"/>
    <dbReference type="GeneID" id="9183982"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006148001"/>
    <dbReference type="eggNOG" id="ENOG502SG61">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148205_0_0_1"/>
    <dbReference type="InParanoid" id="D5GDC5"/>
    <dbReference type="OMA" id="YLQMSGM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038882">
        <property type="entry name" value="Rcf3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39153">
        <property type="entry name" value="AGR244WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39153:SF1">
        <property type="entry name" value="AGR244WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="112" mass="12623" checksum="0AF1884407E570CD" modified="2010-06-15" version="1">MPNRPDPHAGNPDHAALEATKGAVSGAIRWGAVSFLAGVAAYFSSPIFRNLTVQFKVYLWMCPTVVGSMIEADNRLRGYEGMIRRRRRAAMEDARERAYVREIEELERRRGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDX6</accession>
    <name>D5GDX6_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nucleolar protein 9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5 6">Pumilio domain-containing protein NOP9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00006325001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">RNA-binding nucleolar protein required for pre-rRNA processing. Involved in production of 18S rRNA and assembly of small ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430153">
        <property type="protein sequence ID" value="CAZ82719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838528.1">
        <property type="nucleotide sequence ID" value="XM_002838482.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDX6"/>
    <dbReference type="FunCoup" id="D5GDX6">
        <property type="interactions" value="875"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GDX6"/>
    <dbReference type="GeneID" id="9184514"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006325001"/>
    <dbReference type="eggNOG" id="KOG2188">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008720_1_1_1"/>
    <dbReference type="InParanoid" id="D5GDX6"/>
    <dbReference type="OMA" id="PRMESEF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040000">
        <property type="entry name" value="NOP9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13102">
        <property type="entry name" value="NUCLEOLAR PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13102:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22493">
        <property type="entry name" value="PUF_NOP9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="46"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="366"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="49"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="74"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00030932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ82719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="498" mass="55196" checksum="4FACF0AC1424EF2E" modified="2010-06-15" version="1" fragment="single">MPVSTCPLKQDEKQCRVRANDVPSLACISAMRLNKQTIIPPQISFANFKKTNPPTERTSTPENMPKEAKKRGRRAEMKRKEAAKKEQPENTPQVVALAKEAGHFYGVYDGGEVGGEEDTHFYGLLTEDESEYFRKADEILELNQFGGENERALFIANVWKEADGKELKIANSQGCSRLLEKLILMSSPSQLRSLWQKFTGHFLNLAQHRFASHCCEMLFQRSVGIASKETQDDYVDDTATDVFFASMESSFLCMLSELEPQLKHLCTDRFASHIVRGLLLLLSGQPVSSSDLVQSRKKERITLSAGVAPIPEPAEKIPVPQSFHDAVGKILAATAGSMSTEEIRSMAVHQIGGPTLQVLMQLEMGRSRKDRKTKGASKEGTLLGKLTGISTEDEKSGESESSSFFNNLLYDPVGSHLAEKIVVCAPKKVFNKLYNAFFKGRIGSLARNETAGFAVQRILERLEKHMLEEALGEILPQVKGLIERSQVAVVKALIDCCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GE71</accession>
    <name>D5GE71_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00006405001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
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    </evidence>
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    </evidence>
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</entry>
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            <taxon>Tuber</taxon>
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        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
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            <authorList>
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                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
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                <person name="Cantarel B."/>
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                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
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                <person name="Ghignone S."/>
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                <person name="Iotti M."/>
                <person name="Marcais B."/>
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                <person name="Wincker P."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">Mel28</strain>
        </source>
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            <end position="143"/>
        </location>
    </feature>
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            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAZ83026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="256" mass="28606" checksum="D1A1131676F4E4C8" modified="2010-06-15" version="1">MAMATTTVVGPHPLRTNSPAVEKKLECLPTGKSKMAAKPTAAKHKFSSIDGPQIYYDGEAQQTFYDCWTTLNSKRGLLRKEMMTIRRKRVMTLPTHNYGYDDDSEEEGSDKESDQGPKETEEERLKREEEERKKAEEERLKAEREKKAAEVLEYIDSCLDKASKGCENAAFLWLKGDICAGHISFINSRMQEAVDRINLEIDKMKPVAPPAPAPAVEDEGIGAEMDIDADDTPTTIALPPRKMDTAMHDSRVVGVS</sequence>
</entry>
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                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
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                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
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                <person name="Artiguenave F."/>
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                <person name="Da Silva C."/>
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                <person name="Ghignone S."/>
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                <person name="Marcais B."/>
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                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
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                <person name="Wincker P."/>
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            <dbReference type="PubMed" id="20348908"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="eggNOG" id="ENOG502S8Z9">
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    </dbReference>
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    </dbReference>
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            <begin position="100"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="108"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="144" mass="15915" checksum="55376EE3750CA776" modified="2010-06-15" version="1">MLELENKALRRRLDILIPPRPPTEVPTPSFDEHTIQLLAEQEHLRSDLERLTASLGEMEIQQGMLLMNESLRMKEEVAGLRAAIGGIRMQIHWLMNTRHQAVDQRPVGGASSSNGSQNSSSQGGGGALPNITRMADPSNDRVKL</sequence>
</entry>
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        <lineage>
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        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
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                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
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                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
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                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
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                <person name="Wincker P."/>
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            <dbReference type="PubMed" id="20348908"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="KEGG" id="tml:GSTUM_00006875001"/>
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    <proteinExistence type="predicted"/>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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        </location>
    </feature>
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            <begin position="8"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="30"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="187" mass="21316" checksum="964F9C3A2C328FFD" modified="2010-06-15" version="1">MVRYPHPSPRRHQRRRTTTAGSPTRQQTQPGRGWMRSGTSKTRRQKNASQETPLSRRFPIHSTQVPVNQIHPSHKGTIAGLRPNQKIGRHYTTPPSYKYEYSTEISDPAPYPTRILCNHNRTTPPRPAEKKKEKKGKPYLISIFRTISVSRAIPLRGTVIAASADIVNRRPQSTSTYDTGTESPRWV</sequence>
</entry>
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    <accession>D5GFM0</accession>
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            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
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    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
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                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
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                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430209">
        <property type="protein sequence ID" value="CAZ83313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839122.1">
        <property type="nucleotide sequence ID" value="XM_002839076.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFM0"/>
    <dbReference type="GeneID" id="9188261"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006981001"/>
    <dbReference type="eggNOG" id="ENOG502SWER">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_953738_0_0_1"/>
    <dbReference type="InParanoid" id="D5GFM0"/>
    <dbReference type="OMA" id="CKSSCRT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072761" evidence="2">
        <location>
            <begin position="18"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="270"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ83313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="292" mass="31731" checksum="E26283473F47B973" modified="2010-06-15" version="1">MRKSLISAFLFLTLARARVSFNTTVKGFDYDGIVPATGLSESCAEAYAARVSCTDHLFKLRDLEKGSKLFRTKELAQFCTQDCVDSLNEWDTNLQGSCDDRDKNFANSLGSGVYLGMALKDKHSVQENLYWSFCLKDEKSGEKNDFCLAKSDDLPAWPSNTAGNASDTALISDFCKSSCRTQQSYLMHRSAKHLGFSAIDLADAEKICPGLDVSTFPVSIEMLDAFGSSADPSPGRPDASKQAGEDAGQIEGTEYDWDFYKSDFGSGTAASLRVSSAFAFFVGVTAIIAISL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFR7</accession>
    <name>D5GFR7_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein bL33m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00007028001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial ribosomal protein bL33 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430220">
        <property type="protein sequence ID" value="CAZ83360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839169.1">
        <property type="nucleotide sequence ID" value="XM_002839123.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFR7"/>
    <dbReference type="STRING" id="656061.D5GFR7"/>
    <dbReference type="GeneID" id="9187280"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007028001"/>
    <dbReference type="eggNOG" id="ENOG502S7R4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1751031_0_0_1"/>
    <dbReference type="InParanoid" id="D5GFR7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.120">
        <property type="entry name" value="Ribosomal protein L33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052008">
        <property type="entry name" value="Mitoribosomal_protein_bL33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038584">
        <property type="entry name" value="Ribosomal_bL33_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47037">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L33, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47037:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL33M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="23"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ83360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="149" mass="16844" checksum="6930ADD90A328515" modified="2010-06-15" version="1">MTPLHTLSHPANPIPMSSPPKNTPTLPTPSQISPFSRLSHLSPVPTHPLIPTLTPNSKNRVTFPLLLKSNQTSSNHFHPPIPIHLPSQKIMAKKAKSRNIVVRLISMAMTGYFKSFVRPRTHKPLSMIKYDPVVKRRVLFLEQKRGKNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFX9</accession>
    <name>D5GFX9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATP-dependent DNA helicase II subunit 2</fullName>
            <ecNumber evidence="4">3.6.4.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">ATP-dependent DNA helicase II subunit Ku80</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">GSTUM_00001928001</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Single-stranded DNA-dependent ATP-dependent helicase. Involved in non-homologous end joining (NHEJ) DNA double strand break repair. DNA-binding is sequence-independent but has a high affinity to nicks in double-stranded DNA and to the ends of duplex DNA. Binds to naturally occurring chromosomal ends, and therefore provides chromosomal end protection. Required also for telomere recombination to repair telomeric ends in the absence of telomerase. KU70, of the KU70/KU80 heterodimer, binds to the stem loop of TLC1, the RNA component of telomerase. Involved in telomere maintenance. Interacts with telomeric repeats and subtelomeric sequences thereby controlling telomere length and protecting against subtelomeric rearrangement. Maintains telomeric chromatin, which is involved in silencing the expression of genes located at the telomere. Required for mating-type switching.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ku80 family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="4"/>
    <dbReference type="EMBL" id="FN430225">
        <property type="protein sequence ID" value="CAZ83422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839231.1">
        <property type="nucleotide sequence ID" value="XM_002839185.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFX9"/>
    <dbReference type="FunCoup" id="D5GFX9">
        <property type="interactions" value="128"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GFX9"/>
    <dbReference type="GeneID" id="9181769"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001928001"/>
    <dbReference type="eggNOG" id="KOG2326">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010975_1_1_1"/>
    <dbReference type="InParanoid" id="D5GFX9"/>
    <dbReference type="OMA" id="WAMQYVW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043564">
        <property type="term" value="C:Ku70:Ku80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00873">
        <property type="entry name" value="KU80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000073">
        <property type="entry name" value="ATP-dependent DNA helicase II subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1600.10:FF:000002">
        <property type="entry name" value="X-ray repair cross-complementing protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.240">
        <property type="entry name" value="Ku, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006164">
        <property type="entry name" value="DNA_bd_Ku70/Ku80"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024193">
        <property type="entry name" value="Ku80"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036494">
        <property type="entry name" value="Ku_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005161">
        <property type="entry name" value="Ku_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014893">
        <property type="entry name" value="Ku_PK_bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016194">
        <property type="entry name" value="SPOC-like_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12604">
        <property type="entry name" value="KU AUTOANTIGEN DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12604:SF4">
        <property type="entry name" value="X-RAY REPAIR CROSS-COMPLEMENTING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02735">
        <property type="entry name" value="Ku"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03731">
        <property type="entry name" value="Ku_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08785">
        <property type="entry name" value="Ku_PK_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00559">
        <property type="entry name" value="Ku78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101420">
        <property type="entry name" value="C-terminal domain of Ku80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100939">
        <property type="entry name" value="SPOC domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="14" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0347">Helicase</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0779">Telomere</keyword>
    <feature type="domain" description="Ku" evidence="20">
        <location>
            <begin position="286"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00024890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00031847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM00559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="CAZ83422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="665" mass="74337" checksum="BAEF36DC132715CA" modified="2010-06-15" version="1">MADKQATVYVIDLGPTLSRVHPSRGECDLDYALTYIWDKITATMSTGRKTDVIGVVGFRTENTDNAMATADGYENICVLAPISQFLLDHLRDLKRALVPSRGKGKGGDGFSAICIAVDMIMRYCRKLKYIRNIVLVTDGRGEWDDEGCEDIVGQIQEEGINLTALGVDFDDEEYGFVEEGKVPKKARNEKVIKKMVEACNEGGQELGIFGTLAEAISELGKPRLKKIRPKRLRPLSPSTSSDILALLSRNPSVRAPILPKLRGWDPVNARAYQIEDGTTAGGMMDIEKEQMEKGYNYGRTIVPISATDEAITVLETEPGLRIIGFVPVEKYRRYFSMSTTNAIVGAKDNLKASMALSSLIHALFELESLIDVQVPFSEDVRHYRFPPLDKVKTVSGKVLEKHRFLPTPHVEKLMSDYVDKMDLMVANEDEGEPEYATVDETFSPILHRINQIVRWRAVHPDEPLPLAHDILTKYSYPPKTLLGVSTNALQKLIEASDVKRVDPKLAGRKRGREQPAPKSGLDVEALLTSAAPSVPKKAKISSANPIPDFKNKLRTTEDLSDLKEAGKEMLAVLKELIKYSLADANYGRVCEILKVMREEYIEYDEPGIYNIVLQRLKEAIVSEELDGDRMECWDGIRYNRLGLITKGESERVEVDDAAARRFLWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>D5GG25</accession>
    <name>D5GG25_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00001933001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430232">
        <property type="protein sequence ID" value="CAZ83468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839277.1">
        <property type="nucleotide sequence ID" value="XM_002839231.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GG25"/>
    <dbReference type="GeneID" id="9181812"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001933001"/>
    <dbReference type="eggNOG" id="ENOG502S8CY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_370143_0_0_1"/>
    <dbReference type="InParanoid" id="D5GG25"/>
    <dbReference type="OMA" id="LIGCESE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902412">
        <property type="term" value="P:regulation of mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045342">
        <property type="entry name" value="Etd1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20162">
        <property type="entry name" value="Etd1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="162"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="260"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="529"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="172"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="267"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="300"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="531"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="549"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="600"/>
            <end position="612"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="752" mass="81765" checksum="3789FC27009AE37B" modified="2010-06-15" version="1">MLAPSVETPDYDAASTFSGAASLFYDSRPGTRIGDFSHSRVRPSTSTNMTAPKRHVRRSLSISNILPINGPRKRGQHDKGLGGEEEGSDGLVGLYSTDLDVSRRFSEPLVDSPYLLLPRPVSPCMGGEERDPLPPIQSLSALELDMEALPAMNANDLDDYHLETLSPVDPSYEADTDGDEEEEEDSEPFPQGGRHHQHRLSVISASERASTLVGSEDLGHFEGDDFQSETVFDSVRTRVSEPTPVRLDAIFHLPPLEPSEYHLPMGSTKSPMSSDFTPTKGNDVPSSSTPTILTPHIQRSSMSDDGSSSDWDLPSKSADDPGESRAPTSGALRPLSGLHLSSRLGLNLNNASNTSFGTALEGLSIDSSSIRETSSILEWSESIPNNGTPPSLSRRSKAIHAKESLLSGNRVGRRVPSFHIRSQSVPVVSTARTVPSPSENWDDDFLDDDGGDGFELARRGMTIPREIEERQASVIGHLGCIREFALLVEDLKRLRDIGNASGLRNGANKHLLEEADGIIALATLDDEDTLPSVQPDRSQNRSSWHTPSRHSEMTFDDDFKSTPRNRRRSSILLPDDDVFGGGNLPTTPSSFPHLMHGFRSPSASSSPSPLSKKSIDKNDPVEVAKGIMERMQLQHWQTSDHPIGSGKSKGTWNGGKVQFDTDMLRELVVHVGGLKRNLAELVDCLDSPSLKKHPHYIATSDVVESPTALEAGEYGLEISPGMGPFDQESYGNGLEKVRSNNGVDFRPIVGVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGA0</accession>
    <name>D5GGA0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00007285001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430253">
        <property type="protein sequence ID" value="CAZ83543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839352.1">
        <property type="nucleotide sequence ID" value="XM_002839306.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGA0"/>
    <dbReference type="STRING" id="656061.D5GGA0"/>
    <dbReference type="GeneID" id="9184605"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007285001"/>
    <dbReference type="HOGENOM" id="CLU_475821_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGA0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ83543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="573" mass="64418" checksum="87212750F1A0A3EE" modified="2010-06-15" version="1">MNSSYGIHYPLVTQAFLDYYSQRSFLWSQTPVETYIDVEEELELEENNFLEAIWTPLIRVDHASIFSSFPVHAFARYASIATMQNLQLKVGLVKEICAQILKRFEELKEKAGQDGKIDEKALVAALFASSWLCEYHAEGDAITKFKKQVETSAELIGMGRDIEGCLERFSALGEQCAVQRSVAVQDGMVDGLKFRKPWAGFLAEYQDPKKKKKEEHDAQWCTLAKEFIRLQSISVQVPLTERFSDKMRLSLKTELSNIYSGLDEIVRRSPLYVFLSVFPWLPASLHNVLPSQLPKLSSEKFAAYPALLRLHQARSDPPSTLPILQSLLATTRTHSFRDLELPTHILLAKQTFTLQSLTATLSHCEYVVAFLPPSPLLLPHHLPTLLHITFLAALAHTHLRAWELALSTFNVALSLAMHLADTTTQYCILRKLSAIKRESGLGPPVAQTLLRALDLSYATTSQPAFTDECGVLLQRILCAARSGKAGESMENIVERFARKRGWAVSEMKNFVEDVITLEAKIVRGGAEVGARSAETERELRGMYAEAEKMMFARKRRNRAESESGTSITSYTVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGK7</accession>
    <name>D5GGK7_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein SDS23</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Protein sds23</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00007540001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">Involved in DNA replication and cell separation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the SDS23 family.</text>
    </comment>
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        <property type="protein sequence ID" value="CAZ83737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002839500.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGK7"/>
    <dbReference type="FunCoup" id="D5GGK7">
        <property type="interactions" value="200"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GGK7"/>
    <dbReference type="GeneID" id="9181707"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007540001"/>
    <dbReference type="eggNOG" id="KOG1764">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024459_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGK7"/>
    <dbReference type="OMA" id="DWTQISI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051286">
        <property type="term" value="C:cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="F:protein serine/threonine phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042149">
        <property type="term" value="P:cellular response to glucose starvation"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="P:negative regulation of isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="P:positive regulation of conjugation with cellular fusion"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:positive regulation of flocculation"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:positive regulation of mitotic metaphase/anaphase transition"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902472">
        <property type="term" value="P:regulation of mitotic cytokinesis, division site positioning"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="entry name" value="CBS_pair_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050511">
        <property type="entry name" value="AMPK_gamma/SDS23_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016711">
        <property type="entry name" value="Ssd23"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780">
        <property type="entry name" value="AMP-ACTIVATED PROTEIN KINASE, GAMMA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780:SF36">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018148">
        <property type="entry name" value="UCP018148_CBS_YBR214w"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0129">CBS domain</keyword>
    <keyword evidence="6 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="12">
        <location>
            <begin position="120"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="12">
        <location>
            <begin position="195"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="385"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="400"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="416"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR018148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ83737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="433" mass="47004" checksum="63DD0A136B32D8C2" modified="2010-06-15" version="1">MADILSSPPKQEGVPLRDWRAVKVGELLSNKELLFVDLDTPVESACQTLIHNELSSLPIRSSPNAESVCGTFDYSDLTAFLLLIMGLYQPDEDEDMSSFEELARKARAGATIPVKLVKDLGKKDPFITVPETEGLAKVVEVFGSGVHRIAVVREGTNQVIGMISQLRLISFFWEHGRSFPAIDQLYPRSLRDLNIGSGEVISINGDKKVLDALELMNSEGVSSLAVVDNQHNVVGNISTWDVKYLTRSSSMPLLKSPCLHFLSVILTDRGLSDGKDSYPVFHVNAQSTLAHTVAKIVATQAHRMWIVETPSPASTAPPTPTIPPAVAVPPLSVLSPLPSSSSFPITAPTTLTAGALPGAHLSGRLMGVVSLTDILNIFARSAGLTPVDPNEARRQRRRSSSSSMRASFDQGSRSSLDLRELRESSRSSFEIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>D5GH27</accession>
    <name>D5GH27_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007672001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430297">
        <property type="protein sequence ID" value="CAZ83820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839629.1">
        <property type="nucleotide sequence ID" value="XM_002839583.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GH27"/>
    <dbReference type="STRING" id="656061.D5GH27"/>
    <dbReference type="GeneID" id="9185074"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007672001"/>
    <dbReference type="eggNOG" id="ENOG502RZVD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022564_1_0_1"/>
    <dbReference type="InParanoid" id="D5GH27"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031355">
        <property type="entry name" value="YBL010C/LAA2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38698">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38698:SF1">
        <property type="entry name" value="FUNGAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17104">
        <property type="entry name" value="YBL010C_LAA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="321"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="625"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="171"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="326"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="354"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="387"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="429"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="442"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="454"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="631"/>
            <end position="650"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="735" mass="80074" checksum="92AB2465EC02CB4E" modified="2010-06-15" version="1">MVLAICPFSLLDSNDPSGQTLTSEIDNYKRRGRYIEGKMDPPTTPESGLFHPARDSMQVHSETHSGYTSSNEDHFSDAPEGPDAVAEVDNDKRLPTSPPLPPSLPSIPLVLVEKIDERPSHGEVPGTGAFEGRTAGARPDVIRIQPLPDLLAAVPRAFGDNPEGEEQEKEEESKGEVETSRSDVPITIVERVGSTPAHGEIPGTEAFAKRLVDAAPDVIRTATTSPIPTTVVSIVDSDKPLHGEVLGTEAYKMRAADAQPDVVVKWNEEEDKIARRVKSERERIEKLMMNPGRARSVSPGQGPKSVAPAVKGRVDSVSITLPIGEPEGKEGKEKEINGVEKGGKEDDTVKEQPLRSQSPISSQKCGPPVPVGEPEITHDAPIGAGVERAKEETKEVPAEESGAEDAKEEVKGQVIDGVNAGKNGKVEEREEDEAAEEEEKEETTAKEDEIRGEEAEDDDGFGDDDFDDFGEVVEGEEFDDFGGFGEAEAFEPPPVEAAPPPAPPQIPVIPVPVLDFERLDDEDTIRRAAAEAMAIMFPIDKEKYKSITPISIDDNAFLTERSLSLWNQLSAPPPLQPPNWKMSRIRRLFLVSLGVPVDLDEILPAETKQKKLVLPSVNIARKSIDESAGRRNSRSRSRARRSTSSSRRKSGVIGPEALDVPGARILCSTSDVALWGLTVEELKEHIKKLESITCTASEVLTYWLKKREGAISDKETFETVIESLVGYARKKRQNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>D5GH38</accession>
    <name>D5GH38_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">T-complex protein 1 subunit epsilon</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">CCT-epsilon</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00007689001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430297">
        <property type="protein sequence ID" value="CAZ83831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839640.1">
        <property type="nucleotide sequence ID" value="XM_002839594.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GH38"/>
    <dbReference type="FunCoup" id="D5GH38">
        <property type="interactions" value="1266"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GH38"/>
    <dbReference type="GeneID" id="9185085"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007689001"/>
    <dbReference type="eggNOG" id="KOG0357">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008891_7_2_1"/>
    <dbReference type="InParanoid" id="D5GH38"/>
    <dbReference type="OMA" id="SHPQMPH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03339">
        <property type="entry name" value="TCP1_epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000003">
        <property type="entry name" value="T-complex protein 1 subunit epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000049">
        <property type="entry name" value="T-complex protein 1 subunitTheta, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012718">
        <property type="entry name" value="Chap_CCT_epsi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02343">
        <property type="entry name" value="chap_CCT_epsi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 10" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ83831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="550" mass="59762" checksum="9DBF1E8CA52A34EE" modified="2010-06-15" version="1">MAGQMQIDMSNAQMVRDENGRPFIIVRDQGSKKRQHGTEAVKSHILAAKTVANIVKSSLGPRGLDKILISPDGDITVTNDGATILGQMEIENHVAKLLVELSKSQDDEIGDGTTGVVVLAGALLEAASDLIDKGIHPIRIADGFDLACDVAVAHLDRISDVVEFSKDNLDNLTRAAKTSLGSKIVSIAHDKFSKIAVDAVISVADLDRKDVDFELIKVDGKVGGALDDSMLVKGVIIDKDMSHPQMPRVVEDAKIAILTCAFEPPKPKTKHKLDITTVEEFKRLQAYEKSKFEEMIKQIKDTGANLAICQWGFDDEANHLLLQNKLPAVRWVGGPELELIAIATNGRIVPRFEDLTAEKLGRAGVVRELSFGTTRDRMLVIEECANTRAVTVFVRGSNKMIIDEAKRALHDALCVVRNLVKDNRVVYGGGAAEISCSLAVSEEADKTPGLEQYAIRAFANSLDAIPMALAQNSGLSPIETLASVKSRQVREGNSRLGVDCMMTGNNDMRDLFVIDPLIGKRQQLLLATQLCRMVLKVNNVIIAGSGENEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHE7</accession>
    <name>D5GHE7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00007838001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430318">
        <property type="protein sequence ID" value="CAZ83940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839749.1">
        <property type="nucleotide sequence ID" value="XM_002839703.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHE7"/>
    <dbReference type="GeneID" id="9183594"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007838001"/>
    <dbReference type="HOGENOM" id="CLU_2591520_0_0_1"/>
    <dbReference type="InParanoid" id="D5GHE7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ83940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="80" mass="8790" checksum="60DC034292CA0292" modified="2010-06-15" version="1">MVTTFETAVISNATQKTPIARGQLSGKWGSILLSFHQSPHISTLASSRLALLVASALIGPFDSPVAYCFRLMHYFPPWVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHN0</accession>
    <name>D5GHN0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00008052001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="FN430320">
        <property type="protein sequence ID" value="CAZ84060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839869.1">
        <property type="nucleotide sequence ID" value="XM_002839823.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHN0"/>
    <dbReference type="FunCoup" id="D5GHN0">
        <property type="interactions" value="79"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GHN0"/>
    <dbReference type="GeneID" id="9183714"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008052001"/>
    <dbReference type="eggNOG" id="KOG0923">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001832_7_2_1"/>
    <dbReference type="InParanoid" id="D5GHN0"/>
    <dbReference type="OMA" id="FYTREPE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005684">
        <property type="term" value="C:U2-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000001">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000007">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000594">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF83">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR ATP-DEPENDENT RNA HELICASE DHX16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="32"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="221"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="609"/>
            <end position="633"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ84060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="633" mass="71128" checksum="423541472C21F627" modified="2010-06-15" version="1">MEHQLKAAENKARTIEETRKSLPIYEWRTQFLEALEQFQVLIIVGETGSGKTTQLPQYLHEAGYTKKGLKVGCTQPRRVAAMSVAARVAEEMGVKVGNEVGYSIRFEDATSDKTIIKYMTDGMLLREFLTEPDLGGYSALMIDEAHERTLSTDILFGLVKDIARFRPDLKLLISSATMDAQKFSQYFDDAPIFNIPGRRYPVDVHYTQQPEANYLNAAITTIFQIHTTTPPQGDILVFLTGQDEIDAAEQNLQETCRKLGNKIREMIVCPIYANLPSEMQAKIFEPTPEGARKVVLATNIAETSLTIDGIVYVIDPGFVKENVYNPRTGMESLVVTPCSRAAAKQRMGRAGRVGPGKCFRLYTKWAYQNELDENTTPEIQRTNLNSVVLLLKSLGINDLIEFDFMDPPPAETLIRALENLYALGALNDKGELTKIGRQMAEFPTDPMLAKAILAADKYGCVEEVLSIIAMLGESSSLFYRPKDKKFHADQARQRFTKKDGGDHLSLLNIWNQWVDTNFSYNSTQLIQKAITSGFFPNAARLQRSGDSYRTVKNSQTVHIHPSSCLFEVNPKWVIYYELVLTSKEFMRNVMPLQPEWLVEVAPHYHNKKDLDTLGTNKKMPKVQGAAAEKAPPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHP7</accession>
    <name>D5GHP7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008081001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430320">
        <property type="protein sequence ID" value="CAZ84077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839886.1">
        <property type="nucleotide sequence ID" value="XM_002839840.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHP7"/>
    <dbReference type="STRING" id="656061.D5GHP7"/>
    <dbReference type="GeneID" id="9184170"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008081001"/>
    <dbReference type="HOGENOM" id="CLU_424644_0_0_1"/>
    <dbReference type="InParanoid" id="D5GHP7"/>
    <dbReference type="OMA" id="HELDEIM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="380"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="96"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="255"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="311"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="513"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="531"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="569"/>
            <end position="587"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="645" mass="69917" checksum="6D65F738C8BCD4E7" modified="2010-06-15" version="1">MPRPSRVSRKPPAGKEVEKASKKVATTAGKKERKAVEKEIIEKPVAPPARATRSRGTVEVVIETSQPSQTAVASTRTSPDRFKPTTTRRRGQSQQPVAAITTFPAAPAAETSPPKRSTRSTVNFTATSTTITIEEVIRKTPSPKKPTRAAAKVPLHKQQKSNSGVLATPPMPEITPLSPEIQVPASSDVENHSPAGSAHFASSPPAILGTAPCDDDEAGPTTPTQARGGDGKRERIHLEFGEDEGYTPPRARPSSAERPRLRELGVESLEPLDRLPGDDVASSGWFAEDDEDRPASPTPKRANRWKLSQLAEEEEEEAEAGEEADLEDNDGIPAYARSEQQRRKKRFGSPFQFQKGKKDALPPTAAVTAIKVEDMAFAIYSDPNTPSPTLPDNINAHELDEIMRPPISSSPAQESPRHSRKRRSSSADVSSDDEEGDGVEEHHNQTRGGKRRKQTLTRSKPAAAPKPKVLQTEDLRGLLPKRKGRGKHAERDEFGLSPSSSEGDGEEDELSKPTKRKSKPAKKSKSKTKPKSSATTAAKVTKSAWSRPLVPPTTTATTTIRKKYARKSTGSEKENEAPRGNRNKNDDGVGEEDEAATTTSGTVVEVADGVLGKEGRKKLRDAAEKFRIVDSWEMDFEDVSGSTSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>D5GI78</accession>
    <name>D5GI78_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00008322001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430324">
        <property type="protein sequence ID" value="CAZ84221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840030.1">
        <property type="nucleotide sequence ID" value="XM_002839984.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GI78"/>
    <dbReference type="GeneID" id="9181939"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008322001"/>
    <dbReference type="eggNOG" id="ENOG502S9V3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1251469_0_0_1"/>
    <dbReference type="InParanoid" id="D5GI78"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="2">
        <location>
            <begin position="14"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ84221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="221" mass="24672" checksum="5D23F7E87DCD86A3" modified="2010-06-15" version="1">MTTTLFPTTYPTGKFILAASTCTYHIPTSSIVLVHKHHSPSTPRTSWDWFLPRGRKDINESIHATAIRETSEESGYTPTLLATSHNTLQPGSDEEHAEAFHMQLIPYFKRAGVCMYVAFYYLATIADVVRAEDYGDGFDGRHEAGYESEVVKIEEAVEVLSGGKYVLRDGEVYDIVEKRMVGVDGVVDRFFEVGVVVRGWREVQKHFAKAGGQGVQLGDHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIL3</accession>
    <name>D5GIL3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00008549001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430327">
        <property type="protein sequence ID" value="CAZ84356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840165.1">
        <property type="nucleotide sequence ID" value="XM_002840119.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIL3"/>
    <dbReference type="GeneID" id="9183290"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008549001"/>
    <dbReference type="HOGENOM" id="CLU_1107779_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIL3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072811" evidence="2">
        <location>
            <begin position="20"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="199"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ84356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="251" mass="25204" checksum="33B09010D75FBAD5" modified="2010-06-15" version="1">MKLTLASLLALNAVIAVHGRAIPETQPEAIWDRLVGRFASSFEAHRHNNRAVFPEYKPSGTQGVVPSAGTTGRPTGTGTGPIGTGAVGTGTSVLAGTGTGIVSGTGIPTYGAGTGIFIGTGTRGGVYPTGTGVIRPPTTSIPRQIPTSIPSQIPTSIPGQIPTSIPEPSQKPNTTSKSPQPSTGVPPGATDPVPLPQCSGATSAAETRTVTQPAVTVTQPPVTVTMPVTVTAPAVTVTVASGECYDDSETY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIL7</accession>
    <name>D5GIL7_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Amine oxidase</fullName>
            <ecNumber evidence="9">1.4.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00008554001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>L-topaquinone</name>
            <dbReference type="ChEBI" id="CHEBI:79027"/>
        </cofactor>
        <cofactor evidence="9">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="9">Contains 1 topaquinone per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>L-topaquinone</name>
            <dbReference type="ChEBI" id="CHEBI:79027"/>
        </cofactor>
        <text evidence="8">Contains 1 topaquinone per subunit.</text>
    </comment>
    <comment type="PTM">
        <text evidence="8 9">Topaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the copper/topaquinone oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.-" evidence="9"/>
    <dbReference type="EMBL" id="FN430327">
        <property type="protein sequence ID" value="CAZ84360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840169.1">
        <property type="nucleotide sequence ID" value="XM_002840123.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIL7"/>
    <dbReference type="STRING" id="656061.D5GIL7"/>
    <dbReference type="GeneID" id="9183294"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008554001"/>
    <dbReference type="eggNOG" id="KOG1186">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015739_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIL7"/>
    <dbReference type="OMA" id="CMFEIDK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008131">
        <property type="term" value="F:primary methylamine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009308">
        <property type="term" value="P:amine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.20:FF:000002">
        <property type="entry name" value="Amine oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.20">
        <property type="entry name" value="Copper amine oxidase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000269">
        <property type="entry name" value="Cu_amine_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015798">
        <property type="entry name" value="Cu_amine_oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036460">
        <property type="entry name" value="Cu_amine_oxidase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016182">
        <property type="entry name" value="Cu_amine_oxidase_N-reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015800">
        <property type="entry name" value="Cu_amine_oxidase_N2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015328">
        <property type="entry name" value="DUF1965"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10638:SF20">
        <property type="entry name" value="AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10638">
        <property type="entry name" value="COPPER AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01179">
        <property type="entry name" value="Cu_amine_oxid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02727">
        <property type="entry name" value="Cu_amine_oxidN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09248">
        <property type="entry name" value="DUF1965"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00766">
        <property type="entry name" value="CUDAOXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49998">
        <property type="entry name" value="Amine oxidase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54416">
        <property type="entry name" value="Amine oxidase N-terminal region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0186">Copper</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="4 7 9" id="KW-0801">TPQ</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072363" description="Amine oxidase" evidence="10">
        <location>
            <begin position="23"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="domain" description="Copper amine oxidase N2-terminal" evidence="12">
        <location>
            <begin position="89"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1965" evidence="13">
        <location>
            <begin position="238"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="Copper amine oxidase catalytic" evidence="11">
        <location>
            <begin position="317"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="390"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate; via topaquinone" evidence="7">
        <location>
            <position position="471"/>
        </location>
    </feature>
    <feature type="modified residue" description="2',4',5'-topaquinone" evidence="8">
        <location>
            <position position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR600269-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR600269-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF09248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ84360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="778" mass="86543" checksum="BF050ECFBCA31CF0" modified="2010-06-15" version="1">MVGLGRILACLAVITSELVVYAAPSCSPRLNPRSTHLYPRDEICTAPFNPSVTAPKPNPWNPLSNAEVINLLAWLHAPAQGLNLTAFDNASAWDNRVEVTELLPPNKTDVLTYLDGSGPAPKRYARVVISFGATENPYWQEFMVGPIPVVQSVTKIEPLNYLYNKGEGKSPNYAADDKVRRAWLGLIALNMSDITLDILGTDYGNSSLWGIDPLWEENGRIINWVQFWSNPTGGYDSSTLLPQGLYLKLDITGRDMSGWKLLNILYDNKLYKSIDEFRAAWKSPGFRKLAKQADGEWAGTGQTGTAPPLDIRSPPIQIQPDGQRFAVDKDAKYVEWMDFAFYIGFTRDTGVNLYDVKYKGKRIIYELGLQEAIAHYAGNDPTQSGTSYIDSFYGFGPNVFELVPGYDCPAYSTFLDSTVHVDGNTTTHKNSICLFEHDAGYPLRRHTAASYVSVTRNTIFTLRFVATIGNYDYDFDYNFYLDGSIEIKVRASGYIQSAFYAHNEDYGYQIHDATSGSMHDHVLNFKADIDILGTKNTFEKVGIVPATTDYIWSTSPRNTMRLERSSVKNEDEGKINWATNGATMYVVVNKDQPNKFGSSPGYRIMPGAGTPIHLTIQNSSNLQKAAEFATHHLYVTKHKDTELHSSTAANSMNPKDPLINFSKCFDGESLLQEDIVVWFNLGMHHVPHTGDLPNTVFTTAQSSVFLSPHNYLLSDPSRATSQQVEVNSDTGDAGVTVNTFGSTLPSCTRPDLAVLYPDLCSYQGPQRTRKFPYDGNPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIW3</accession>
    <name>D5GIW3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00008712001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="CAZ84456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_002840219.1"/>
    </dbReference>
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    <dbReference type="STRING" id="656061.D5GIW3"/>
    <dbReference type="GeneID" id="9185674"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008712001"/>
    <dbReference type="eggNOG" id="ENOG502RBV5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006014_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIW3"/>
    <dbReference type="OMA" id="VVGWDAY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04401">
        <property type="entry name" value="RhoGAP_fMSB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037508">
        <property type="entry name" value="Msb1/Mug8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012965">
        <property type="entry name" value="Msb1/Mug8_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28093">
        <property type="entry name" value="MORPHOGENESIS-RELATED PROTEIN MSB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28093:SF1">
        <property type="entry name" value="MORPHOGENESIS-RELATED PROTEIN MSB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08101">
        <property type="entry name" value="Msb1-Mug8_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="41"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="11"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="364"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="435"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="546"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="596"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="660"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="965"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="364"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="436"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="546"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="660"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="679"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="754"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="965"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="984"/>
            <end position="993"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ84456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1018" mass="111458" checksum="EEC20C41E4817CFB" modified="2010-06-15" version="1">MPTFLNKVFKRDHAGSSKKIEPSAPPPPQEVKHQDAWLRTEVTPEQVQELLRGCTQEIKSRGLSTPFLLLPFRPNSDASAARTFIRNFFDPVTGPLRGPHLQQELRLTEPVVLCSTLKWCWSRLPGGVVTWDLYELFRQGEIGSGMARDSFATFIPISAESEARSKIIFDFFDLISAIAAHGKHNGLGGMKLSRLAGWWAFEHSDMGWGFEGGYTSWSRAADATMHLFFAYLRSLSPADGTRGVSVLPISLKALVASVDYPPTTPVQLQSQTVRVVMTVETVSPTPFSLLRRARNFQFRESNLALRALVGYDDPIQALTEECKRVLKCISSTNQSSSGFKSGSGLQDQSWSRFQDLGFSGLLEESEGSADEDGLSMTGSETFPRRRASTQPIPSTPHQELAMDIARPTTPSWADFMAAGFGDRDQGPNQPAPLLLPPDKILPPIDSGSRVRSSQSNRKIAEDNLDPGELASVSPLTVDDAFWWVWISSLAGEESTARKAVFGRCALVETVIKGGRWLVVEEKVKGAATALEDATYLLAREKKKSRRGKLTRRKSAGRADIPIEQPKQFDPSIGPFSRINIRPDQMARVQTAAAALRQQQIQQEPSHLRPREVGSMPNPRTNSVFTLQPVILSEATPAMKWASKYDKDAIREAYLKGLPMPERKDLSRVDTDRPLPPPPPHEEPEERPERQERQERPKPELVGPKPEQLTQPPTPVTKQAPVALASPRTPSAPRSVPMTLPSPRSNGRASPAFDSRSQTTPSSPKSQPSANPTPEKSKKPKASRFKNLFSSKKADPTSLVGRRISQLEPPNSGGLGVPTQDTSRRLSVGRKKNAPIDFSGGAAQLNAPPPLVIEKPSLSLQADLRRQDSDQPSTSQVSSKEERAATQVFSSFDQGPLTDQPAFVPDDSVMTSESGSPIESGPAAPEKETDDVVSMDGTAPLASPVEAIPVQDRWAQIKKNAAERAKVVPKASDEYTRSDARGSIDDGETSGEETIESRVARIKARVAELTGSIDTTTRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIW5</accession>
    <name>D5GIW5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00008714001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the MEF2 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840267.1">
        <property type="nucleotide sequence ID" value="XM_002840221.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIW5"/>
    <dbReference type="STRING" id="656061.D5GIW5"/>
    <dbReference type="GeneID" id="9185676"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008714001"/>
    <dbReference type="eggNOG" id="KOG0014">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024080_1_0_1"/>
    <dbReference type="InParanoid" id="D5GIW5"/>
    <dbReference type="OMA" id="LGRYQYF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008301">
        <property type="term" value="F:DNA binding, bending"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033554">
        <property type="term" value="P:cellular response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00265">
        <property type="entry name" value="MADS_MEF2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1810.10:FF:000013">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033896">
        <property type="entry name" value="MEF2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11945">
        <property type="entry name" value="MADS BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11945:SF534">
        <property type="entry name" value="MYOCYTE-SPECIFIC ENHANCER FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00350">
        <property type="entry name" value="MADS_BOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="8">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="75"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="228"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="506"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="75"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="156"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="175"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="228"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="242"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="325"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="521"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ84458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="563" mass="59806" checksum="B9B3BDA3ED2721D9" modified="2010-06-15" version="1">MGRRKIEIKAIKDDRNRSVTFLKRKGGLFKKAHELSVLCSVDVAVIIFGHNKKLYEFCSGDIREVIGRHSYYEAPHEHKGPADFQGKKDAMDDDDDDMDIPSRPGSRSSNDHPMPHPLQSLGHMGNRHSTSASPPIPPSTLMGGQGTYMQGRVGTPQPMSRPSSRNQRRNSSNMVPPSTYASSQGPPAQNGFPYMPSGPVFTPQPQPVMPMQQGNMNQMPVYPYPQTTQAQAQAQAQAQAYINETNRRQSMPPTFPSERPVPSSENSPPNRPLDEQVAPAIEKRAPAKSRSMFTPIGPSESLLAQHFGFGQEPSRSHSVDVGSVARSSSTSGMNNVSVKSEKQSPKPAPASVPAPTTSSNANGQQPSVEPVPPPPSRTNSMASNPGKPGVRPRLKVQIPSEHSDAGDATAESSPRESAGTTTGQSVKGVGEGNHPVVLPPPSPSVTLRSAGASGGPVNPFARPPPATTGAPSETPISALPSRFCDNGLLPSPSTFYQDWGIGFGTSASRAGGNSDMLPSPLNFQTPVVSTGRNIFGEDSDVNGKRKSPGSDNSDGSAPKRVKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIY1</accession>
    <name>D5GIY1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00008734001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840283.1">
        <property type="nucleotide sequence ID" value="XM_002840237.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIY1"/>
    <dbReference type="STRING" id="656061.D5GIY1"/>
    <dbReference type="GeneID" id="9185692"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008734001"/>
    <dbReference type="eggNOG" id="ENOG502S5MV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031138_1_0_1"/>
    <dbReference type="InParanoid" id="D5GIY1"/>
    <dbReference type="OMA" id="PTEYSKD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071008">
        <property type="term" value="C:U2-type post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000390">
        <property type="term" value="P:spliceosomal complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012890">
        <property type="entry name" value="GCFC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028211">
        <property type="entry name" value="Ntr2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12214">
        <property type="entry name" value="GC-RICH SEQUENCE DNA-BINDING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12214:SF0">
        <property type="entry name" value="LD29489P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15458">
        <property type="entry name" value="NTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="303"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ84474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="423" mass="46801" checksum="A808340161D1E6F4" modified="2010-06-15" version="1">MRSTFASRRPKTRVVARDDESEEEEQTKVVKAPPKSTEQSQGSLSRPKGKKRSSLRLSFGSDASIVDDEEREVFALRKSTLSRKAIEKNAARKSGLSAGVIGMEHLNLTPSIDRPTYSKEHLDELRSNTPTIPLSQQADLEVEDVDMDVVGADVPETNENTTLTKYGESSKQETIIPDEGLVRVMKARRAEKAAKAKAEDYISLNIGSDGGSGEILLKSKKKESRIQRADDNLYDEEELVKYIEDGGIVLGGKAAQRAHDRKRRENIREAIKEAEGIHDDAMDMDSDSRDSLVEEWERDQIRKGDFSNSSGGGIARDLEMLSRQPPDVTPLPNLDDALRRLEETLAAMEFKKAQTVRQMEALQAEKQEISEREAMVQQRLQKASEEYEKLSGGLGIGGGGGASAVGRGLESFGNTPIRVDRET</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIZ8</accession>
    <name>D5GIZ8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00008756001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840300.1">
        <property type="nucleotide sequence ID" value="XM_002840254.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIZ8"/>
    <dbReference type="FunCoup" id="D5GIZ8">
        <property type="interactions" value="940"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GIZ8"/>
    <dbReference type="GeneID" id="9181832"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008756001"/>
    <dbReference type="eggNOG" id="KOG0710">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046737_1_1_1"/>
    <dbReference type="InParanoid" id="D5GIZ8"/>
    <dbReference type="OMA" id="QKHENIQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06464">
        <property type="entry name" value="ACD_sHsps-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031107">
        <property type="entry name" value="Small_HSP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11527">
        <property type="entry name" value="HEAT-SHOCK PROTEIN 20 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="4">
        <location>
            <begin position="40"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ84491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="149" mass="16677" checksum="9C594A694D7EDBD1" modified="2010-06-15" version="1">MPFFLTSFNTFSLTTRANTAQAPLERLLTRASSPSHLPALAPPTTTPKFDVRELPSAYILEGEFPGIDDTSSLCIEFTDRSTLLIHGRLERAVTEPAAEAERGNYLVMERSVGVFQRRFWFEEGVEVEGVDASLGNGILRVYVPKRERE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJ39</accession>
    <name>D5GJ39_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Methylthioribulose-1-phosphate dehydratase</fullName>
            <shortName evidence="7">MTRu-1-P dehydratase</shortName>
            <ecNumber evidence="7">4.2.1.109</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">MDE1</name>
        <name evidence="9" type="ORF">GSTUM_00008825001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the dehydration of methylthioribulose-1-phosphate (MTRu-1-P) into 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>5-(methylsulfanyl)-D-ribulose 1-phosphate = 5-methylsulfanyl-2,3-dioxopentyl phosphate + H2O</text>
            <dbReference type="Rhea" id="RHEA:15549"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58548"/>
            <dbReference type="ChEBI" id="CHEBI:58828"/>
            <dbReference type="EC" id="4.2.1.109"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 2/6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the aldolase class II family. MtnB subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.109" evidence="7"/>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840341.1">
        <property type="nucleotide sequence ID" value="XM_002840295.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJ39"/>
    <dbReference type="FunCoup" id="D5GJ39">
        <property type="interactions" value="236"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJ39"/>
    <dbReference type="GeneID" id="9181873"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008825001"/>
    <dbReference type="eggNOG" id="KOG2631">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006033_4_0_1"/>
    <dbReference type="InParanoid" id="D5GJ39"/>
    <dbReference type="OMA" id="WFPGTSG"/>
    <dbReference type="UniPathway" id="UPA00904">
        <property type="reaction ID" value="UER00875"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046570">
        <property type="term" value="F:methylthioribulose 1-phosphate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.225.10:FF:000003">
        <property type="entry name" value="Methylthioribulose-1-phosphate dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="entry name" value="Class II aldolase/adducin N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03116">
        <property type="entry name" value="Salvage_MtnB_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001303">
        <property type="entry name" value="Aldolase_II/adducin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017714">
        <property type="entry name" value="MethylthioRu-1-P_deHdtase_MtnB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027514">
        <property type="entry name" value="Salvage_MtnB_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03328">
        <property type="entry name" value="salvage_mtnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10640">
        <property type="entry name" value="METHYLTHIORIBULOSE-1-PHOSPHATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10640:SF7">
        <property type="entry name" value="METHYLTHIORIBULOSE-1-PHOSPHATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00596">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01007">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="AraD/HMP-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="1 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 7" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Class II aldolase/adducin N-terminal" evidence="8">
        <location>
            <begin position="22"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="7">
        <location>
            <position position="133"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ84532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="233" mass="25738" checksum="06F34D00A3B5F9DE" modified="2010-06-15" version="1">MMSQGNSREELVISEDPNHPANLICSLCKNFYSLGWVTGTGGGISIRHESHVYLAPSGVQKERINARDIFVMDFPTKTYLRRPPSLKPSACTPLFMASFTKRNAGACIHTHSQAAVMCTLLSKGSTWSIANVEQIKAIPRPSTGGYYGFFDKLVIPIIENTAHEEDLTERLGKAIDEYPDTCAVLVRRHGVYVWGDTVWKAKTQAESLDYLFQLAVEMVKLGMDPAGPITVAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJ54</accession>
    <name>D5GJ54_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008843001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840356.1">
        <property type="nucleotide sequence ID" value="XM_002840310.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJ54"/>
    <dbReference type="STRING" id="656061.D5GJ54"/>
    <dbReference type="GeneID" id="9181888"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008843001"/>
    <dbReference type="eggNOG" id="ENOG502SANZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059580_1_0_1"/>
    <dbReference type="InParanoid" id="D5GJ54"/>
    <dbReference type="OMA" id="ETKHRVY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046591">
        <property type="entry name" value="DUF6649"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20354">
        <property type="entry name" value="DUF6649"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="195"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="237" mass="26410" checksum="73B67F5941C4B577" modified="2010-06-15" version="1">MRIPYEKGRSKGRENHHYRPVMEWSTDFQRKRPPSDDLLEEQPLSKRLEKLSLVKKVELLNKPRLFPTTTPSSGTPLSQTEAERMDVDNVIFIKSLDEEIASYDSEDDPDSRKLIFLSDVEKQLTRVPYLLAESGAHSGGGGGGETSTDLVLYSVPSSLSVSEERDSVRRVIIEARNRIRKRQADEAAAAVTQAAGVVMGGEEEEEEKGYEWDSMETGGDIAGGRGFYEDPDAMDIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJD1</accession>
    <name>D5GJD1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00008944001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430330">
        <property type="protein sequence ID" value="CAZ84624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840433.1">
        <property type="nucleotide sequence ID" value="XM_002840387.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJD1"/>
    <dbReference type="STRING" id="656061.D5GJD1"/>
    <dbReference type="GeneID" id="9185992"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008944001"/>
    <dbReference type="eggNOG" id="ENOG502S1X9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_619929_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJD1"/>
    <dbReference type="OMA" id="FENHERC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052073">
        <property type="entry name" value="Amide_Lactam_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47171">
        <property type="entry name" value="FARA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47171:SF6">
        <property type="entry name" value="SPECIFIC TRANSCRIPTION FACTOR, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G06130)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="8">
        <location>
            <begin position="26"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="143"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="158"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ84624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="442" mass="48450" checksum="572440FA245E9C99" modified="2010-06-15" version="1">MGATVRVQVFAANNSSYRDRKRAHRACDQCKRRRKRCLPPFENHERCAGCTRDGLYCSLVAVELSQGGGSATAGAPVNPAEVESEGFTDKLAVSSDREGQSRFIGDLNPEAALLSATSNLPSGPSSSSAPALDSNEIGVWVSHPQAVPRSRKRRKSPDTTQPSTGTEPTLATHYNNFLESLNAFSRPPRPSVDALLLLYMESVHPVYPLVDTYTISLFKTPDAIPPVLLLSILLVASRHPKAAEHLVLPPTNALLAPREFASTLYTRITALLHTGNEKDRIRLIRVYGLLSLHSADGPHGNEVASMNLCTAIHHAHSAGLHLKRDRNGSSGETELWWCLWELDKLQAAINGRPVLVRGEDTSIDFPTRDEGTGVFRGMVRLAELLEEVIALYRPRKEKEGEAFRWEEDFPSFEQVVGETNCGDQGLLGISRPIPPLPSSETN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJP3</accession>
    <name>D5GJP3_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">mRNA export factor GLE1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Nucleoporin GLE1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00009098001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GLE1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430332">
        <property type="protein sequence ID" value="CAZ84736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840545.1">
        <property type="nucleotide sequence ID" value="XM_002840499.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJP3"/>
    <dbReference type="STRING" id="656061.D5GJP3"/>
    <dbReference type="GeneID" id="9186770"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009098001"/>
    <dbReference type="eggNOG" id="KOG2412">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_490185_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJP3"/>
    <dbReference type="OMA" id="ILWAKYR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044614">
        <property type="term" value="C:nuclear pore cytoplasmic filaments"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000822">
        <property type="term" value="F:inositol hexakisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.510">
        <property type="entry name" value="GLE1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012476">
        <property type="entry name" value="GLE1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038506">
        <property type="entry name" value="GLE1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12960">
        <property type="entry name" value="GLE-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12960:SF0">
        <property type="entry name" value="MRNA EXPORT FACTOR GLE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07817">
        <property type="entry name" value="GLE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="7" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0811">Translocation</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="144"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00026227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ84736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="556" mass="62677" checksum="DA05FA09DCA62456" modified="2010-06-15" version="1">MPLPDSDSEGEWEEGNSDADLFANSGGGIYSSPIRTASPRGAQSTCAVRAHNLAEKINALDLKAKEYSLASKLVATDDKANQEFMLVYLQDTRFDAREAAILEERFQAKVRRVYEAKRQDDLNRARIEKEYREKLAREAEEAARRAGEIARQQEMERQRHEQEKREQEQRAAEAARLQAERVRAEEERKRQADLEKEEALKRQKDAEEAKVKAERDSKERAVREAAAALHAASRSGPRVYGRKSTTYERVAMQRVIASLHQLRHTVLQEEAFSKEKKLTTIRRGLRPKFGQLNGERNQTIEVRDFLKNTFVDLRSDQEGPMISANDFFLNQDPSCDVPVPVAFVWTVNELAKLLVKQVVNECAMKPVTADPIGVAVISTFADERLLVNGHSFIDIVIARLLKKTPILTGELGPETTEGDRKRLGWIQGGDGTWETEENHVNRMVGLTAGYTAIAGRNFGNSKLINPYPIFNLWHAVATVLNAPADKLTNTHYFVMKTFLEVGAKKLVDVYGHQAKKLVELAVGDWALRGESQGFSGAVSVKAFGKFLRGEEWWKNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJW0</accession>
    <name>D5GJW0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00009230001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430334">
        <property type="protein sequence ID" value="CAZ84803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840612.1">
        <property type="nucleotide sequence ID" value="XM_002840566.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJW0"/>
    <dbReference type="FunCoup" id="D5GJW0">
        <property type="interactions" value="503"/>
    </dbReference>
    <dbReference type="GeneID" id="9186839"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009230001"/>
    <dbReference type="eggNOG" id="KOG3662">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011607_0_1_1"/>
    <dbReference type="InParanoid" id="D5GJW0"/>
    <dbReference type="OMA" id="LHCMKYP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033308">
        <property type="entry name" value="PGAP5/Cdc1/Ted1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13315">
        <property type="entry name" value="METALLO PHOSPHOESTERASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13315:SF4">
        <property type="entry name" value="METALLOPHOSPHOESTERASE, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="383"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="6">
        <location>
            <begin position="106"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ84803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="484" mass="54804" checksum="BF7BC672F7F77F12" modified="2010-06-15" version="1">MASKQVQWPQRLASFPTLIWPHLTMVNLLRLVWLSTVYYDERQVYKSAMAACYWNNWENWGPETVPHRIVLVADPQIVDRHTYAHRGIALSAAIFYTDLYMSRSYDSIIDHLNPSTVIFLGDLFDGGREWELTDDYWLSEYARFSKVFPSTPYRRTVQSLPGNHDIGVGNGIKESVLERFRLFFGEGNSLLALGNHSIILLDTPSLLNDVNPRIHNPPRDFLDSLPDLLSPNPPLPPHIIQNSSIPASPSPEQTNLNPVILLSHIPLHRPADTPCGPRRQSQKPIRIGGGYQYTNTLPELLSQEILHKTGAKHVFSGDDHDSCTVAHEYDDGKQKAEEVTVRTFAWTMGVRQPGFLMVSLLNDGLRDGKETVLVHECLLPDQIGIYLGYVELVVHTLVIVLLNMVRRRGGVWWWCRCLRPRGGEGEDKGKREGLLGIVQSERGAGMEGGVGKKQNRECCREFFGDLRDLVIVVVPFYLALLVLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJY9</accession>
    <name>D5GJY9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00009276001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430335">
        <property type="protein sequence ID" value="CAZ84832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840641.1">
        <property type="nucleotide sequence ID" value="XM_002840595.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJY9"/>
    <dbReference type="GeneID" id="9186868"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009276001"/>
    <dbReference type="HOGENOM" id="CLU_2924400_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJY9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ84832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="61" mass="7118" checksum="1EF11D2689BAC5FB" modified="2010-06-15" version="1">MESVRIECASRPTIQVAANSECIAYADKRFRIRPSLKRIEEHQKFEATITHEQSRNWAGQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>D5GK72</accession>
    <name>D5GK72_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00009405001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430337">
        <property type="protein sequence ID" value="CAZ84915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840724.1">
        <property type="nucleotide sequence ID" value="XM_002840678.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GK72"/>
    <dbReference type="STRING" id="656061.D5GK72"/>
    <dbReference type="GeneID" id="9184684"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009405001"/>
    <dbReference type="HOGENOM" id="CLU_706346_0_0_1"/>
    <dbReference type="InParanoid" id="D5GK72"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="391" mass="42359" checksum="48EE36CA6F94CC8B" modified="2010-06-15" version="1">MARPSCPKPILREKGPRCHPSTLSFPTASASNSKTQHASQLALISHTLSPLHTLLSQPIPFVKTQTISGTLAECQTVLSLGYFGREMGRKRSLASAEGKAIKRVRRKGSTNNVNSRDLGGDELLEQPQPVSERKVVGEVSVGKTTLETPARKIKLPQPGKSTIPPKDKKNKPNKELANTTSTKSKANGGATSKPVPGSQKKVIKKNGLKDVPHLPESGVPSGQSTGSTVLLPDGATAPGMTIVERDSTSKSQFKNLKLSTGSIANTSGKTVVSGTSNPDRKTEALSERYSGEYPDKQRVKGIQNRSAFCYRNSVLQLLASSEVCVQEMIGHIEMHIEESCRYPILILPVSGMSNLFKVAVVLIVFPVRLENSSRTISLLPEWIRTRTHVYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKS4</accession>
    <name>D5GKS4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00009741001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430340">
        <property type="protein sequence ID" value="CAZ85117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840926.1">
        <property type="nucleotide sequence ID" value="XM_002840880.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKS4"/>
    <dbReference type="GeneID" id="9183353"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009741001"/>
    <dbReference type="HOGENOM" id="CLU_2741892_0_0_1"/>
    <dbReference type="InParanoid" id="D5GKS4"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="71" mass="7821" checksum="9595E94D854F8913" modified="2010-06-15" version="1">MSYKLPYCKLEELAVLVGGRDEKDRGPSKELGNGNGNGMHHTKAAGEEKRETEGKERMKGTLIIIPETSFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKW6</accession>
    <name>D5GKW6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00009799001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430341">
        <property type="protein sequence ID" value="CAZ85159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840968.1">
        <property type="nucleotide sequence ID" value="XM_002840922.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKW6"/>
    <dbReference type="GeneID" id="9183395"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009799001"/>
    <dbReference type="HOGENOM" id="CLU_2575590_0_0_1"/>
    <dbReference type="InParanoid" id="D5GKW6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="81" mass="8684" checksum="57F881981C38D3FB" modified="2010-06-15" version="1">MVPVVVSVGPGPFSSHPSVTVTDSRTGKEADSSILTASHSSFSLKLLPHQLHHTLALLILSFSITVYLTTLSIHFSTAKFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GL00</accession>
    <name>D5GL00_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="13">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00009855001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-cystine(out) + H(+)(out) = L-cystine(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:66172"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:35491"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:66173"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cystinosin family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430342">
        <property type="protein sequence ID" value="CAZ85193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841002.1">
        <property type="nucleotide sequence ID" value="XM_002840956.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GL00"/>
    <dbReference type="FunCoup" id="D5GL00">
        <property type="interactions" value="199"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GL00"/>
    <dbReference type="GeneID" id="9185787"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009855001"/>
    <dbReference type="eggNOG" id="KOG3145">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046327_0_1_1"/>
    <dbReference type="InParanoid" id="D5GL00"/>
    <dbReference type="OMA" id="LAFAYHG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015184">
        <property type="term" value="F:L-cystine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000016">
        <property type="entry name" value="Cystinosin homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005282">
        <property type="entry name" value="LC_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00951">
        <property type="entry name" value="2A43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13131">
        <property type="entry name" value="CYSTINOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13131:SF5">
        <property type="entry name" value="CYSTINOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00679">
        <property type="entry name" value="CTNS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0458">Lysosome</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0769">Symport</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="23"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="56"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="101"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="199"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="237"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="268"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="332"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ85193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="375" mass="42082" checksum="70C05AC76DBAEF11" modified="2010-06-15" version="1">MTILSSTPPDERVVELAHAISRLLGWTYFLCWGVSSYPQLLINYSRKSVTGLAFDFFTVNILGFGCYTISSILFLFSPVVRDEYARRHPRSPEPTVRWNDLAFAIHATLFSVITWSQFFFWGYKRDPTQRLSRTMLALIAMCIVTILVSIALALSGIADFEWIDVAYTLQFVKLFISIVKYVPQAWLNYKREATTGWSIHNILLDISGGVLSLAQLVLDSSLQNDWSGLTGNPLKLFLSQISIFFDIIFIIQHYVLYRSSSESSTYSHLSPEGEAEIGYGTLEPPPRIPRAGGDKRSRSSPLCRSFGEEEQRSFGRFRHGSEGEGAPNELGLRGREKKDYGVGDKRSDEEERGGLLGSSFSETFTRVGDTGATGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>D5GL23</accession>
    <name>D5GL23_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00009924001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430344">
        <property type="protein sequence ID" value="CAZ85216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GL23"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009924001"/>
    <dbReference type="HOGENOM" id="CLU_3432054_0_0_1"/>
    <dbReference type="InParanoid" id="D5GL23"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ85216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="17" mass="2114" checksum="18D9A381F6F4F8B4" modified="2010-06-15" version="1">MERKKREGDEGERLAYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>D5GL64</accession>
    <name>D5GL64_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00010025001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430346">
        <property type="protein sequence ID" value="CAZ85257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841066.1">
        <property type="nucleotide sequence ID" value="XM_002841020.1"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GL64"/>
    <dbReference type="GeneID" id="9182051"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010025001"/>
    <dbReference type="eggNOG" id="ENOG502SCKR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_708213_0_0_1"/>
    <dbReference type="InParanoid" id="D5GL64"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="211"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="159"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="184"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="236"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="294"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="314"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="372"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="390" mass="42115" checksum="047915B769CF87BE" modified="2010-06-15" version="1">MPRALPWALNRPPTSASSSSKPSTKPPPPKTKPKRNPSTPPPHHNAPRREGSALDEAIIEYMTPQDDIYIMVEDELLTTAQLYTRSLHRAEYSRLQAAAALKNAHRIKDIHRPVDGVTRMSREAIMRGRRGARVAALGLDGGGSSSSDEDEEGWGKGALGRLMSGRSSSSGYAKGRLGERWKGRGGGGGGGGTKAAAGFTDAXSGALSIISQDIPVEKEKEGRNASSCPKIKQEQDSDTDTPSDDDLDAPVHSFRTPSIPPLSSISSFANNPNQAKPIKRLPCERRDSIPPFISASSTSFSSTTTTSTYHTTSAPQSRATSFSKIIDLDDDPLFRLPKPPEKSSTYRGKSMAARRRAEGENSRSGTGGGDFGNKREKEKERRPFDSLRSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>D5GL88</accession>
    <name>D5GL88_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00010073001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430347">
        <property type="protein sequence ID" value="CAZ85281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="tml:GSTUM_00010073001"/>
    <dbReference type="HOGENOM" id="CLU_3435096_0_0_1"/>
    <dbReference type="InParanoid" id="D5GL88"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ85281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="14" mass="1751" checksum="54D876C827E978A3" modified="2010-06-15" version="1">MYSSTWKTYQTNKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLC9</accession>
    <name>D5GLC9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00010135001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430348">
        <property type="protein sequence ID" value="CAZ85322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841131.1">
        <property type="nucleotide sequence ID" value="XM_002841085.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLC9"/>
    <dbReference type="SMR" id="D5GLC9"/>
    <dbReference type="FunCoup" id="D5GLC9">
        <property type="interactions" value="743"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GLC9"/>
    <dbReference type="GeneID" id="9184777"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010135001"/>
    <dbReference type="eggNOG" id="KOG0118">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025000_1_2_1"/>
    <dbReference type="InParanoid" id="D5GLC9"/>
    <dbReference type="OMA" id="NEIRVNW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12614">
        <property type="entry name" value="RRM1_PUB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12619">
        <property type="entry name" value="RRM2_PUB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12622">
        <property type="entry name" value="RRM3_PUB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000117">
        <property type="entry name" value="Nuclear and cytoplasmic polyadenylated RNA-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000224">
        <property type="entry name" value="Nuclear and cytoplasmic polyadenylated RNA-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000134">
        <property type="entry name" value="Nuclear and cytoplasmic polyadenylated rna-binding pub1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48038">
        <property type="entry name" value="RIBONUCLEOPROTEIN RB97D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48038:SF1">
        <property type="entry name" value="RIBONUCLEOPROTEIN RB97D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="86"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="176"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="302"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="371"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="8"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="36"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="443"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="466"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ85322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="498" mass="53371" checksum="7138835709F01BCA" modified="2010-06-15" version="1">MSADNQNAPPAGSSSIPAPAPSASAPGQNQGQSSHMPPPPLPPVIIPQNTNPIPTAITSPMSVSGVTSPTSAGGFVRRAAPEPNKRALYVGGLDPRVTEEILKQIFETTGHVQNVKIIPDKNSKGYNYGFIEYDDPGAAERAMQTLNGRRIHQAEIRVNWAYQSNQSSKEDTTNHFHIFVGDLSNEVNDEVLMQAFSTFGSVSEARVMWDMKTGRSRGYGFVAFRDRADAEKALSSMDGEWLGSRAIRCNWANQKGQPSISQQAAMAAMGMTPTTPYGHHNFPTHGVQSYDMIVQQTPQWQTTCYVGNLTPYTTQNDLVPLFQNFGYVVETRFQADRGFAFVKMDTHENAAMAICQLSGYNVNGRPLKCSWGKDRPPTGQFDAYSPQQPNTPQYPPQTPSAYFPQYGGPMTPQGPPNSAGGPNALQGHGPQSPAGPSPPGRWQDQSQGFGGPQNQTAYGGMPQSAGGYGRGNGTGTPGGWGGNSAGAFGSNGFGAYQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLF9</accession>
    <name>D5GLF9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ribonuclease H</fullName>
            <ecNumber evidence="3">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00010176001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RNase H family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="3"/>
    <dbReference type="EMBL" id="FN430348">
        <property type="protein sequence ID" value="CAZ85352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841161.1">
        <property type="nucleotide sequence ID" value="XM_002841115.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLF9"/>
    <dbReference type="GeneID" id="9186096"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010176001"/>
    <dbReference type="eggNOG" id="ENOG502RU94">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_483286_0_0_1"/>
    <dbReference type="InParanoid" id="D5GLF9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043137">
        <property type="term" value="P:DNA replication, removal of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09276">
        <property type="entry name" value="Rnase_HI_RT_non_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050092">
        <property type="entry name" value="RNase_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002156">
        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10642">
        <property type="entry name" value="RIBONUCLEASE H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10642:SF26">
        <property type="entry name" value="RIBONUCLEASE H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00075">
        <property type="entry name" value="RNase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50879">
        <property type="entry name" value="RNASE_H_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase H type-1" evidence="9">
        <location>
            <begin position="348"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="34"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="45"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ85352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="564" mass="62395" checksum="C76A97F9F01B0E96" modified="2010-06-15" version="1">MPRIPIVSAPRGVLSRLLAKKVAVKQALAVPRGGLRGRTNANKQTIDERKGRGERGEEMENKEGGSMVWRPVDWTKRGSSAGLEAGEYWPKLVGVEEGHAQISIARLTNLATRPPSRRTGFSSPRQLRMVPRSSAKISELEERPSLLPKLDASSDTLAAIRLINMAIVLSSSREKALKGIRAVDIRRQGGGFEVVFSSQEEKLTAMKNSSWVSLLSGGSPPETNECGGVRKSFGLVVHSVSCRGFAHAGAKDFIKQLYTENPWDPEDVTVVDARWIGNVKEMASTKRGFRSMILFFKTREAAVTVRESGLFFNKQYRPPENIIPLVSLLAARQSRSNAQAVTKERLEAGREVVVFTDGSKNGLEAGAAVWRKADSRGGEIKKMYGLGTRMTIYDAELYAIWKAIGLGGRVARERGAKKLTICADSQAALGVMARGTIGPGSHLALRGRAQMKDLHHTHGIDVALEWVRSHAGIRGNIRVDKLARAACAIRQKQKDSYTSEILAERRRSREEWVRGCRERRLKRWKAAKTEAEEEMEAKPVNYVVNTGYGKSPFKEYEAPVVVHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLN1</accession>
    <name>D5GLN1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00010279001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430349">
        <property type="protein sequence ID" value="CAZ85424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841233.1">
        <property type="nucleotide sequence ID" value="XM_002841187.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLN1"/>
    <dbReference type="STRING" id="656061.D5GLN1"/>
    <dbReference type="GeneID" id="9182727"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010279001"/>
    <dbReference type="eggNOG" id="ENOG502QSYI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005049_6_1_1"/>
    <dbReference type="InParanoid" id="D5GLN1"/>
    <dbReference type="OMA" id="EWIHESV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018712">
        <property type="entry name" value="Tle1-like_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33840">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33840:SF1">
        <property type="entry name" value="TLE1 PHOSPHOLIPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09994">
        <property type="entry name" value="T6SS_Tle1-like_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="T6SS Phospholipase effector Tle1-like catalytic" evidence="1">
        <location>
            <begin position="13"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="403" mass="44379" checksum="0C4E5AF22C35F799" modified="2010-06-15" version="1">MSNPFSDSGPRMKRIIICCDGTWQDSDGGKLEVPTNVTRVARALKSFTDDGVSQVIYYQSGVGTGGTVNRLIGGGTGFGLSENVREAYGFLAHNYHPGDTIHLFGFSRGAYTARSICGLVCRIGILTKKGMDNFYQVYQDYQAKKFRSQSAVAEFQAREGGSLVIPNVRVKTICCWDTVGSLGIPSTGVPIIGDWIAGNEQEKYGFHDTDLSPRVDNAFHALALDEHRGPFTPTLWKKTDDTPVNLKQCWFPGVHTNVGGGYEDQEIADMTLSWMIQQLLPFLQFDDDYLKIIIHNSDDHSSGEWAAGKIYDSATGIMRIVGGNTRTPGGYGDGTEETIHKSVRIRTKVVSGWSSKALKGWTWNENGYWEKGSKRMAEDQLGSLEKRLAGKAVVKDMLGEDMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMF5</accession>
    <name>D5GMF5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CTP synthase</fullName>
            <ecNumber evidence="9">6.3.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">UTP--ammonia ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00010683001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>UTP + L-glutamine + ATP + H2O = CTP + L-glutamate + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:26426"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the CTP synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.2" evidence="9"/>
    <dbReference type="EMBL" id="FN430354">
        <property type="protein sequence ID" value="CAZ85698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841507.1">
        <property type="nucleotide sequence ID" value="XM_002841461.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMF5"/>
    <dbReference type="FunCoup" id="D5GMF5">
        <property type="interactions" value="861"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GMF5"/>
    <dbReference type="GeneID" id="9182124"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010683001"/>
    <dbReference type="eggNOG" id="KOG2387">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011675_5_0_1"/>
    <dbReference type="InParanoid" id="D5GMF5"/>
    <dbReference type="OMA" id="EFNNAYR"/>
    <dbReference type="UniPathway" id="UPA00159">
        <property type="reaction ID" value="UER00277"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097268">
        <property type="term" value="C:cytoophidium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003883">
        <property type="term" value="F:CTP synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044210">
        <property type="term" value="P:'de novo' CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019856">
        <property type="term" value="P:pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03113">
        <property type="entry name" value="CTPS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01746">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000207">
        <property type="entry name" value="CTP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004468">
        <property type="entry name" value="CTP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017456">
        <property type="entry name" value="CTP_synthase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033828">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003792">
        <property type="entry name" value="PRK05380.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550">
        <property type="entry name" value="CTP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550:SF0">
        <property type="entry name" value="CTP SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06418">
        <property type="entry name" value="CTP_synth_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 9" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CTP synthase N-terminal" evidence="11">
        <location>
            <begin position="1"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase" evidence="10">
        <location>
            <begin position="280"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU810713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF06418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ85698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="511" mass="56892" checksum="302E582299C52D73" modified="2010-06-15" version="1">MNVDAGTMAPTEHGEVFVLNDGGEVDLDLGNYERYLNITLTRENNITTGKIYQHVIERERRGDYLGKTVQVVPHLTDAIQDWIERVARIPVDETSEEPDTCIIELGGTVGDIESAPFIEAMRQLRRRVGKGNFLQIHVSLVPVVNGEQKTKPTQQAIRDVRSAGLAPDLIACRCQEPLSKATVEKVAAFCDVAHEQVVAVHDVNSTYHVPLLLEQQKLIHLLTSVLELEKLSIAPALIQKGIDIWNEWKTLTLAQDRLFDKVDIALVGKYTDLHDSYLSVENPAKFHKAWHDVCTAGGILVPGGFGARGTEGMIAAAKWARTNKIPYLGICLGMQIAVIEYARHVVGLEDAHSVEISASSTNPVVIFMPEGSKTHLGGTMRLGIRPTIWQPNSEWSKLRRLYGEKEAILERHRHRYEVNPEYIERLSGLNFVGKDDKGERMEIVELKDHPYYVGVQFHPEYLSRVLSPSKPYLGFVAASAGVLDQMLEKLQKIQPTVAPAAALVELNGTLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMN0</accession>
    <name>D5GMN0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00010820001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430357">
        <property type="protein sequence ID" value="CAZ85773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841582.1">
        <property type="nucleotide sequence ID" value="XM_002841536.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMN0"/>
    <dbReference type="FunCoup" id="D5GMN0">
        <property type="interactions" value="86"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GMN0"/>
    <dbReference type="GeneID" id="9185109"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010820001"/>
    <dbReference type="eggNOG" id="KOG0191">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021868_0_0_1"/>
    <dbReference type="InParanoid" id="D5GMN0"/>
    <dbReference type="OMA" id="IFVYFYD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd02961">
        <property type="entry name" value="PDI_a_family"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052250">
        <property type="entry name" value="PDI_TMX3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46426">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE TMX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46426:SF1">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE TMX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072155" evidence="8">
        <location>
            <begin position="22"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="619"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="9">
        <location>
            <begin position="32"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="9">
        <location>
            <begin position="196"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="471"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="114"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ85773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="664" mass="74735" checksum="06018497E67DB0DD" modified="2010-06-15" version="1">MRFSTFIPLLFAALAAPLVVGEPIQTARTASVATVAPASAATLPAKVESEVKAPDIIELTTGNFWTTIGKSYWFVKGYSPTCPHCIKAAPIWKQLHDKYATQVVDSGTPPSPNSNPGSPSSSPTFTEKYDFHFANINCVSYGDICADKKVGIEMFPSFILFKDGKAIEQYPIEADRTVEDMSKFVDKYIVEIAAKSNRNLALPKTIYDESKNPEGKSVALAADDFLKEVIGTGDGWFIKFYAPWCQHCQALASTWAELGREMKNQLNIGEINCENDRRLCKELKIRGYPTIIYFQHGERIEYNGLRGLGDLVSFAKKAVDSSIKEIEFTDFEEMEKGGMEVAFLFFYDSATTTEDFKAMERLPLTLIGHAPLLKTKSELLARRFRITTWPRLIVVRDGHPSYFPGISPGAIRDFGRVTGWARTVWLPILPELSASNSHEVMNGKTVVLGILSRDRPKDFAAAKKELKAAALEFMDQRQLQEKNEKQELRDKKQLRIEEAEDRNDQRALRAAKNMKINLEKKKEVGFAWVDGVFWERWVRTTYGINVGEMGERIIVNEEDTKQYWDTTLEGTAIVPSRSTILDTLKAVIAEHPRIRSKSTTNRFETAYYQIRAKVSNHPLVSWFLLASVVIALAVWGRGRMKRRGTGGFFRLESTKEEGVGGKVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMS6</accession>
    <name>D5GMS6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00010912001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430359">
        <property type="protein sequence ID" value="CAZ85819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841628.1">
        <property type="nucleotide sequence ID" value="XM_002841582.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMS6"/>
    <dbReference type="STRING" id="656061.D5GMS6"/>
    <dbReference type="GeneID" id="9185155"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010912001"/>
    <dbReference type="eggNOG" id="KOG2058">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005609_0_0_1"/>
    <dbReference type="InParanoid" id="D5GMS6"/>
    <dbReference type="OMA" id="WQDYLRI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000026">
        <property type="entry name" value="TBC (Tre-2/Bub2/Cdc16) domain family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000046">
        <property type="entry name" value="TBC domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF20">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="2">
        <location>
            <begin position="786"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="339"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="420"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="570"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1047"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1140"/>
            <end position="1179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="448"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="471"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="581"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="651"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1079"/>
            <end position="1105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1155"/>
            <end position="1166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ85819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1179" mass="128081" checksum="6161A6DCB6AB20F4" modified="2010-06-15" version="1">MELNPGPCGDGSPSGSVSETPPADESSESTPTDLHRSIPSQASSTVDLLETVDESHPLSNTGSDDDGPESSLTASVANTTTKSTSPVSQRYIFRPPSDRTSAQQSMTPSATSPLHKSLASPIARPGEQNSDPRSALFDDHVASAHVPGSLHLAPDSAIEKLVERQGVISLIRQLASDLAHRDKELVSIRRRAEERERALKKMLVEVEVSNADIEKRLAAAILQRKPSRETMRSAAGDTSYTESIDDMMQQAMSEEDVFSVVDDSLSMMEATTDLGDDDRHSTRDDMVTPKATIRARSDSRSILFERDMDSMSIISNASSRRGSTVRRWKNFFWGQKIGEGGENNTGNERLVLQNPPHSKRRGITDETFSPPPEGKPRILSRASSPAVSMIQPGSHPDALAQSGTSKVAAWAMRLASHAPPLHEQENFNPKSPVQPRSAGGTEGSGLPADRRASSGSIDRENLQRIMAKNAARTQTSPRRRTASTVSNGAPPGPSKFFQDIIDHAERMVPLGNSSQNASNADPSSGPVEMDRIVPHAIQPPTLLQSWNDYYPTDYLTDRFGFIYDKKNKALSAKSKSSVGGDDAKQPRYEDVKPRSTPGGGDPPNASSGANYGLLDAGDARTPTRWQEPPPETPSISLHQPKTSKEAPASAPENSQHGQPTKSLIDRIAKSTSAMASPTTTTFSEAPLALRSPLHSEMSSIAEASTVQLLLGQLSDLHDSLQRDRGAKWNEFLKKIRQERRRGEEEEKGMPEVLMGDGELIGIATLGNEGRGGKQRWKEFKGLVLGGIPVAYRWKVWTECSGATAMRVPGYYEDILENGLDDPLVISQIEMDINRTLTDNVFYRQGPGVSKLKQVLVAYSRRNSAVGYCQGMNMIAASLLLIMPSEEDAFWVLCSIVEKILPKTYFESNLLASRADQQVLKQYVQEVLPSLHMHLQKLGVELEALTFQWFLSIFTDCLAAEALFRIWDVILCLVGSPFLFQVALALLRLNEKALISCKSAAAVYSYLNGEMTHQGISIDGLIRESDGMKHHVKRKEVELRRERAVEKELGEVGSGDNGGATVEAVKTDSPIRAVANGSDEESRKPSTKSIHDEEREDGNGEDKTKGEAAGVAEVGPSSSAIQEETAEELLAADAKPLVGIIEHLANEDQEPRAVTSPPSDNPATSIPPTEASAIPIVATT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMY6</accession>
    <name>D5GMY6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00011025001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430361">
        <property type="protein sequence ID" value="CAZ85899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841708.1">
        <property type="nucleotide sequence ID" value="XM_002841662.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMY6"/>
    <dbReference type="STRING" id="656061.D5GMY6"/>
    <dbReference type="GeneID" id="9183467"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011025001"/>
    <dbReference type="eggNOG" id="ENOG502SGJS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_276837_0_0_1"/>
    <dbReference type="InParanoid" id="D5GMY6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="447"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="510"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="645"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="728"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="952"/>
            <end position="1017"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1082"/>
            <end position="1109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="63"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="144"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="247"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="323"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="369"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="645"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="728"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="756"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="790"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="952"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="979"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="991"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1004"/>
            <end position="1017"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ85899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1148" mass="125772" checksum="E29FD0493DA2F563" modified="2010-06-15" version="1">MEPTLPARATRPVSRGAKNEGVTRDVKPVVQRKKPEPLPISDDIWELPLSSPVSQEPKVGGRVQEHSAVKGRLMEKGKVVPGNRGRGKGRSYKSEARVVDSSGEEITPHSGSGPVGPVAPRALGGTEIRKSAPTPQPKALTKTVHPTQTSEAQPNLRRSLREKKPPKVVLIPSSGGESEGEELVQYTPRKATTRLLSPGKKQKPATANKRALPETYRTNPPIKKRKDAPIPESIDARRGARVVQVGKKQEDAREPRVVGEKQSAVAQENALGHKTTAMSSKRKAHKPTITPKELSEGSHKRQRTSSPKTVGYGKKGGALAPTLNANDNSGAITSFPQMDVSPEVRDSGRGGPDLDEWAVYNGDTLPPLNKKEYINHKGNREERTSNISGPATPPEPNAHCPQTAQKIFGGLDDASKGAFTLGRESQKCRLSSLEKFPKELPPQEAINLAKGLSRADTHGTYGPSGSSTAPHDPQESLRGNAPAALANISKILTPHPTSYLVDASTSTADLNEDRAMGTSHPWTSSPPPRIYGENVSTIPKQMPGVTSLMAQLKDKVTCDRAVSASAVSETATNGLYLLSPIEFSKENSPTGPKVHNATHDEELSIPDGLSKLPTTQKLQSLVILPEEDEDVVVWDAKEKRGGFTALKRTETRHTEVDENGSPSRQRVPKPPAGPKRVSCEILDEPKDPAPEAPQEPETEENVFVKSTTSILRERTSLGGQLFVEKTPHLVRTRAKGGKHRNPGISTSYEGGRGYFKQKDSATRHIDGPKNKSTELPKPFEKFHEKERDVISISSTSPAPALPIALPPKKSHRVCQEPELREDSDDAESIEPAVARVLKILAETSTSIPKSSPPVLHHSKPKVQLIRNGKRKLGDNNNLVQGARVETISTGDRPFEDTVLARKGHPETHFSKMLRKQRFVRIQNPPYRGNPPNRRLAEEQFRRASRVVPINGEHVRRQERIEDSVQGGLDGDTEVEDSMNAYTDDNGSDINTDTCSDSECSGSGDSDDEDENGEEGEEDAHIIWRKSLPGHLKETLSALEQITRGLVKYLVCSEELAIEMIENFEAQGTRLIKALDNSHTQEYNEFLENLEEAKKAVAAKAEELDQLITNGLDIVERKEEDWKNNQMAKKKQHQELDNAIEEMLMDTDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>D5GN43</accession>
    <name>D5GN43_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00011079001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430362">
        <property type="protein sequence ID" value="CAZ85936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841745.1">
        <property type="nucleotide sequence ID" value="XM_002841699.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GN43"/>
    <dbReference type="STRING" id="656061.D5GN43"/>
    <dbReference type="GeneID" id="9185862"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011079001"/>
    <dbReference type="HOGENOM" id="CLU_000288_125_11_1"/>
    <dbReference type="InParanoid" id="D5GN43"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019894">
        <property type="term" value="F:kinesin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002151">
        <property type="entry name" value="Kinesin_light"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45783">
        <property type="entry name" value="KINESIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45783:SF3">
        <property type="entry name" value="KINESIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0802">TPR repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="7"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ85936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="75" mass="8237" checksum="40AC9A746A9BF23D" modified="2010-06-15" version="1">MYRRALASREKTLGPDHPDTLRSVDSLGAALSNQGKHSEAEIAYRYALASREKTFRLDNPNILRSVNGSGAALHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5GND2</accession>
    <name>D5GND2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00011230001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430363">
        <property type="protein sequence ID" value="CAZ86025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GND2"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011230001"/>
    <dbReference type="HOGENOM" id="CLU_2279496_0_0_1"/>
    <dbReference type="InParanoid" id="D5GND2"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ86025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="102" mass="11311" checksum="0EC03B142E2E601D" modified="2010-06-15" version="1">MTNRWKGQSHDMVWQVEPGSVSRRGPADVTAFFVLMLMAEGKTSAPGFGTVPAFWGIRYCTSTVRILEHSRTIPTYRAQTIAASQSCKAPPRLHREVFASPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNE4</accession>
    <name>D5GNE4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00011246001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430363">
        <property type="protein sequence ID" value="CAZ86037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841846.1">
        <property type="nucleotide sequence ID" value="XM_002841800.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNE4"/>
    <dbReference type="FunCoup" id="D5GNE4">
        <property type="interactions" value="703"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GNE4"/>
    <dbReference type="GeneID" id="9182237"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011246001"/>
    <dbReference type="eggNOG" id="KOG1302">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016678_3_1_1"/>
    <dbReference type="InParanoid" id="D5GNE4"/>
    <dbReference type="OMA" id="EFHIFFV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.850">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.830.10">
        <property type="entry name" value="Syntaxin Binding Protein 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043154">
        <property type="entry name" value="Sec-1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043127">
        <property type="entry name" value="Sec-1-like_dom3a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043155">
        <property type="entry name" value="VPS33_dom3b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005715">
        <property type="entry name" value="VPS45_Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="277"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="282"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ86037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="647" mass="71834" checksum="037905287A026314" modified="2010-06-15" version="1">MAPHSTTIDTAHIKATAQRDLLDLLVAVRGKKTLILDKSLSGPVGLFCKFSALQDHGVDKIFWLDENVVADLTQKNIVFLVRCTVKNALKIAAHVKNNAKIPGQQFEYNVMFVPRRTLVCEKILEDEGVLGDLTIGEFPLHFIPLESDLLSLELEDAFEELYLRKDHTSIFYSARALMNIQRRYGLFPRIIGKGDCARRLADALIRMRGEEDALDSSSSPFALAPSSVLGELIVVDRESDFMTPLLTQLTYEGLIDETIGIHNSKIEVDSSLVGGPQQSGVTAASSSSASPAATSSQQKKKPISLNSEDKLFENLRDTNFAIVGNLLNKVARRLNDDYEGRHQAKTVSEIREFVGKLGSLQQEHQSLRLHTGLAEEIMKHTRSDIFNRILEVQQNLAAGVDPSSQHDAIEELIARNAPLESVLRLLCVESLVGQGLKPKDLDNFKREILQGYGYQHVLTLAALEKLQLLQSRTSITSSTSTRTNYATLRKSLKLIVDEVNEQNPDDIAYVYSGYAPLSVRLVQCVIQKPLMTRGRRGEDPSTPAVVGTAVNWAATGWRGFEDVLKNVKGKTFDELQRGEEKAVRARNLLNGANEKKVTVVFFVGGCTFTEIAALRFVGRREEEKREIVICTTGIISGDRMMRAAMEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNH7</accession>
    <name>D5GNH7_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Methionine aminopeptidase</fullName>
            <ecNumber evidence="7">3.4.11.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00011295001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.</text>
            <dbReference type="EC" id="3.4.11.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="6">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="6">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="6">Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe(2+)-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.18" evidence="7"/>
    <dbReference type="EMBL" id="FN430364">
        <property type="protein sequence ID" value="CAZ86070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841879.1">
        <property type="nucleotide sequence ID" value="XM_002841833.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNH7"/>
    <dbReference type="FunCoup" id="D5GNH7">
        <property type="interactions" value="819"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GNH7"/>
    <dbReference type="MEROPS" id="M24.017"/>
    <dbReference type="GeneID" id="9186145"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011295001"/>
    <dbReference type="eggNOG" id="KOG2738">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015857_0_0_1"/>
    <dbReference type="InParanoid" id="D5GNH7"/>
    <dbReference type="OMA" id="INQGTHQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004239">
        <property type="term" value="F:initiator methionyl aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01086">
        <property type="entry name" value="MetAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01974">
        <property type="entry name" value="MetAP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001714">
        <property type="entry name" value="Pept_M24_MAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002467">
        <property type="entry name" value="Pept_M24A_MAP1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00500">
        <property type="entry name" value="met_pdase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43330">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43330:SF7">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00599">
        <property type="entry name" value="MAPEPTIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00680">
        <property type="entry name" value="MAP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6 7" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6 7" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M24" evidence="9">
        <location>
            <begin position="1"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="239"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="255"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ86070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="264" mass="29154" checksum="C4C2687405786ED9" modified="2010-06-15" version="1">MRKVCRLAREVLDICAAAVRPGITTDELDEIAHKACIERDSYPSPLNYVNFPKSICTSINEVICHGIPDQRKLVDGDIINLDITLYHGGFHGDLNETYYVGDKAKANPDTVRVVETSRDCLDEAIKLVKPGMLFRDPGNVIEKLAKQRNCSVVRTYIGHGINQLFHCNPNVPHYAKNKAVGVAKPGMCFTIEPMINLGTYKDTTWPDKWTSTTSDGKMSAQFEHTLLVTEGGVEVLTARFPDSPGGRMPIPGIDRNGEGGKKQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNJ1</accession>
    <name>D5GNJ1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00011311001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430365">
        <property type="protein sequence ID" value="CAZ86084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841893.1">
        <property type="nucleotide sequence ID" value="XM_002841847.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNJ1"/>
    <dbReference type="STRING" id="656061.D5GNJ1"/>
    <dbReference type="GeneID" id="9186160"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011311001"/>
    <dbReference type="eggNOG" id="KOG1840">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_125_8_1"/>
    <dbReference type="InParanoid" id="D5GNJ1"/>
    <dbReference type="OMA" id="HYILAEG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056681">
        <property type="entry name" value="DUF7779"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053137">
        <property type="entry name" value="NLR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46082:SF6">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46082">
        <property type="entry name" value="ATP/GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25000">
        <property type="entry name" value="DUF7779"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13374">
        <property type="entry name" value="TPR_10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NB-ARC" evidence="1">
        <location>
            <begin position="19"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7779" evidence="2">
        <location>
            <begin position="231"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ86084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="828" mass="93816" checksum="028D826CF3F008E8" modified="2010-06-15" version="1">MVAFCRNDTFTGRKDIIARLKELLEDQNYNRVALYGLGGAGKTQIALEYVHQRKGLSHVFWVHGSSFLKFGEDYRRIFQTVGISQGGPEPSENEQLSEVKRWLESPASGSWIMIIDNADNEADFSGNKSPISRFVPQSSNGKIVITTRSRVVASRQGCSVVEVGKMRTEEARELFFQRFGKTVGLQDHDLEASNKRRKALLSEQFCDIRREVDMTESILSTYFITFERINEKSPKGADLLRLIAFFDRQKIPEELLVKSGLEGMNDPIEFRKAIGALLNFSLVTRAEGAAVYELHRLVQHSIQVYLKQEEVRKWSRTGLSVILRLFPPHEHNVRDICASYLPHALAILENSDDPLVSPVHYRVAMHLQSIGHYNDAEVQIRRCIQLEGDTDENDCGSLARSSLLATVLVARGRYEEAENIFRRVLEGREKVLGSDHLDTLTSAHNLALVLGQLGRHNEAEKLNRALVWILDALGKYDEGERTNKRVLDTRQRVLGPGHPDTLSSLHKHGVMLQRLGRYAEAEKYYRHALEGREKKLGPEHPDTLSSVDGWVMVLLELGEYAGAEQPAQRAFEGYQKIWGPDHKSTLMAMGNLSSVLQGLGRYDEAETVLRTVVEGREKALGPESMITLLSVNNLALLLHHLGQFEESEKLQRRVFETREKTLGLINEATLSSLRNLASVLRDQGKNEETEEVSLRALKCCTELYGEEHLETLTCVENLALFLCYRHRYQDAEKMQRRVFEERKKKLGLDHPNTFVSADNLAAILAGLGMYDEAEEMCRWALEGSERVLGLNHPQTLTNLKNMASLLQRAEGASEAERLPRHLKVMRSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNK9</accession>
    <name>D5GNK9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00011339001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430365">
        <property type="protein sequence ID" value="CAZ86102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841911.1">
        <property type="nucleotide sequence ID" value="XM_002841865.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNK9"/>
    <dbReference type="GeneID" id="9186178"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011339001"/>
    <dbReference type="eggNOG" id="ENOG502S00W">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_337706_0_0_1"/>
    <dbReference type="InParanoid" id="D5GNK9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072224" evidence="3">
        <location>
            <begin position="25"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="259"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="297"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="384"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="762"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="824"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="763"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="844"/>
            <end position="855"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ86102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="855" mass="94308" checksum="E15A65BE42662FAA" modified="2010-06-15" version="1">MALTASKLRASIVIFLALTPRALAADGVDFSNNLFSDLAPVLALFGEQVSKQFLSQSLTWLEDVIFAMAPLGIVTAIVSAVRAGGPGWLKAVVGRARESRAVAEVELMSSTSHEVCELWNGEAIVRVMGSTKFAEILYFQDGADGKGGSKFFTLEAAKENGILINAKKRREDRPTHGEGTVFAGLGLPDRILSTFRGPFVRLDPEGAYTRNATAGREYELRRRHQRAAPTHATPTRDTDPISTPNISLNICGARGQGELWVFAILGIVLQFGCLIFIGVTRYHPKLNWKEEKNSKYAYPLMASGTLLLVVGMMACSRVVQASSEEDIWVSKRSFRILWLQKGDRVNDQLFKSYALFGDKEREELVTSRRRPHSSPRTSSRTKHVIDVILSLSTLTIAGAFIGIAGFVVQFVGIRALHWSASIAQLVVTLTMTTVRTWIRRKMSKRPKAMKLPVDDEIDWVAVRIGASQDALWKPKPNGPEGARDSDPTWEVITLHGYLGYKGAYAGHIAPIGEAQAILGLRKDLGDLMSWDEISYSFASKLVECLQSLLFYFIDSGEIALAKTVTRPAVLHWALPVKVGGTVQYITMTASRGSSFQWNVDTSELCAVISLWCYGVKSKDIQNTYKYWSYRADPETVLRVLGPATHRFVSDCERWMDRGGRFAVVENARLWENTIEGRATEVNAQELSKIITRVRDHTDHTTDPSPPTDDIAPYRITGTKPINIDGCYKSLAFVSTAPLKQMCAQEILSSLLWAIVGIVGSVPDDTQSRSATPSGDHESEVDEQPDPTPENPFLQELAEIITNSGVTSNIQDAYALIIPPFAAADKLPRRPSDGFTQDEIESIRPQPTSDTNLPSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNS6</accession>
    <name>D5GNS6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00011456001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430368">
        <property type="protein sequence ID" value="CAZ86169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841978.1">
        <property type="nucleotide sequence ID" value="XM_002841932.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNS6"/>
    <dbReference type="GeneID" id="9182821"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011456001"/>
    <dbReference type="HOGENOM" id="CLU_219517_0_0_1"/>
    <dbReference type="InParanoid" id="D5GNS6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ86169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="33" mass="4011" checksum="8BD9083B429783FA" modified="2010-06-15" version="1">MLSQGLQATNYKQKKTKESTKKHEYYYQEPKKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNZ4</accession>
    <name>D5GNZ4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00011595001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430371">
        <property type="protein sequence ID" value="CAZ86237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842046.1">
        <property type="nucleotide sequence ID" value="XM_002842000.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNZ4"/>
    <dbReference type="GeneID" id="9183530"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011595001"/>
    <dbReference type="HOGENOM" id="CLU_1138714_0_0_1"/>
    <dbReference type="InParanoid" id="D5GNZ4"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="91"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ86237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="244" mass="27488" checksum="55AB56DD47B23DC3" modified="2010-06-15" version="1">MQEYSNFIPTTVYIKHEIMQPQNPQTPIKTPIPINPSAPRRMRIRNSEFPESPTPDTSHPGIPPRQRSNGTGTQSLRSPSPLVWDQSTCKTTLTPPSTSNSTSMPITPKYYPGSPPQLLPPSGIPTISEVRHPPQCTPNFEEACKTIQALDRLLEEVRLLKADTFFGNLYWSELNIFYRDFKRFLNRESDRFFTFVGGASVATDADYEGLDTVAQSIFDEWNVMKEAILRNDPGEGLDDAIVID</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPA0</accession>
    <name>D5GPA0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00011769001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430373">
        <property type="protein sequence ID" value="CAZ86365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842174.1">
        <property type="nucleotide sequence ID" value="XM_002842128.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPA0"/>
    <dbReference type="GeneID" id="9186605"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011769001"/>
    <dbReference type="eggNOG" id="ENOG502SW4P">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1483035_0_0_1"/>
    <dbReference type="InParanoid" id="D5GPA0"/>
    <dbReference type="OMA" id="MTIYISR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002775">
        <property type="entry name" value="DNA/RNA-bd_Alba-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01918">
        <property type="entry name" value="Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA/RNA-binding protein Alba-like" evidence="2">
        <location>
            <begin position="12"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ86365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="182" mass="19756" checksum="30BEEE7C900F90E1" modified="2010-06-15" version="1">MDSFDPSSIPTVSIISSHSISKKTTRCLATLFPIQPPTTPSVLAVAIVAKSTVASKAITIAEIVKRRISTQGEAWFQYTKLEGTMVELKAPKKSKKEGKGKNKDQRGSQVEKAGEKGAQIGGEEGTDEEDPFEPLKEKTPQKKRKMPLLTIYISRSSIPELAKLYGEQSGSGPVGEAQEQPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPF6</accession>
    <name>D5GPF6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00011823001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="6">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="PTM">
        <text evidence="7">Glycosylated.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the paraoxonase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430374">
        <property type="protein sequence ID" value="CAZ86399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842208.1">
        <property type="nucleotide sequence ID" value="XM_002842162.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPF6"/>
    <dbReference type="GeneID" id="9186633"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011823001"/>
    <dbReference type="eggNOG" id="ENOG502S462">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_590780_0_0_1"/>
    <dbReference type="InParanoid" id="D5GPF6"/>
    <dbReference type="OMA" id="CEDMWLH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004064">
        <property type="term" value="F:arylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002640">
        <property type="entry name" value="Arylesterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051288">
        <property type="entry name" value="Serum_paraoxonase/arylesterase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11799">
        <property type="entry name" value="PARAOXONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11799:SF12">
        <property type="entry name" value="PARAOXONASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01731">
        <property type="entry name" value="Arylesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="441"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="454"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="141"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="311"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="312"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="7">
        <location>
            <position position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602640-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR602640-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR602640-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ86399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="463" mass="51348" checksum="3CB704E3494F5B5B" modified="2010-06-15" version="1">MGIKSILLPAIVIFLGYLLQGHVIPYLHRSYHLMGFSRTLSHLPPEGSCTAAHQDKFLGCEDIHIYKKEKGTTYMFAACLDEYSYENRWRLMSELRAIDNNDRPVPTPAGDKIWRWRLETDEVVELELPGFAEGGDERAWHGFDFVESADGTLSFYIINHKVSGDTIEKFTHKPDAKNHILTHSATFALPENGIAKHPNDVYAYPDPTGKDIFYVTSDHKHDAGIMRTIEDYTRRPWSHLAYYSSETGWKVVKSGLVGANGLAGTKPTNGGPARIYLAELLGGNIMILEHRPDAAGELREIQTIPLGFLPDNPSLSPGETDLYIAGHVTPLQTYKHIYKPGSVRSGSAVARVALSQTGSDFFGGKDEYTSTPVVEEIFVDVEGKLINASSTSIFWDKLPPVGATEEGEVEGGEVAVGDLFITGLTGRGILRCREFKYDASDDPIVNEEQQGDNMGRDDHEEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPP6</accession>
    <name>D5GPP6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00011962001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430377">
        <property type="protein sequence ID" value="CAZ86489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842298.1">
        <property type="nucleotide sequence ID" value="XM_002842252.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPP6"/>
    <dbReference type="GeneID" id="9186721"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011962001"/>
    <dbReference type="eggNOG" id="ENOG502S8WV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_344238_0_0_1"/>
    <dbReference type="InParanoid" id="D5GPP6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070941">
        <property type="term" value="P:eisosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024527">
        <property type="entry name" value="Eisosome1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28298">
        <property type="entry name" value="EISOSOME PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28298:SF1">
        <property type="entry name" value="EISOSOME PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12757">
        <property type="entry name" value="Eisosome1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="179"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="309"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="378"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="499"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="539"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="739"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="801"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="21"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="311"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="398"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="539"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="579"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="628"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="643"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="685"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="715"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="762"/>
            <end position="778"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ86489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="822" mass="88547" checksum="CD827B9334676A6C" modified="2010-06-15" version="1">MTTLLAPPAVSFPTKGPMGWSETSTADTSSKSSISAPTATPTHAPMASTAEDTMTAESQGIPMQTASTQTDNEVLSRATSHAANRANDRNYLQGQAEKPGQESDLDSSPLKPKQGAASHYLRRAEAHELPSFGNVGLANPEAAGAAAASLAHASAKPVEIWKPDPISAAGAAACLAHASPKRVEPWKPTQGTGAGKAARHALEDNNDRITPLTEPQPPERWSLTGTQGSMKVCRAGPSSTLQHDSGTEISGNALTTAPPAHMPMVRRASNAVEPAFEQPVENDLNNAGGVSQQERRKSDILRAATISMVKRNCDTHSPDSPRHRVPSPGRQRPAAPPSSRHQIYGPDLDEAACKVAAARLALIGYDPKRSASLLEAKGAQASLSRSRSVIAEDASDHSVAQRTDGNPSRSHDNLARLENKKRGENAVLLMAAAQRNVQAQMSGLDRQIADSKGLVRREDWEARARELAQASHDKRQHQNQDAIAARNVRPVLDEMTRKAEAERARVSAEKTRAAEERMELEEKKRLQEVWREREKETQEELRRAQAMGKEEGKCRKGQEKRHQKERRHSFRTESKRAKGSNSNNNIQPLQLLAPIVSNSNSNSDDHAPITPPTSPKEEKGLRGLINKFRVRRLSRNARRTSSKEPSSVGATAITKNYPSTNCNTAPSRDPNIPPPSALQTNGSQTPTTTPTLTEVALLGAPPPAPTARLPRNPPHTSSPVSSLHSFQCRGETVLEVHISDDDGSGDAAHNGYSNSSNNPQRRKGEDTRNHSNGAEEHNTFPGTPPALAALNLSGEQLPLEREASAGEPGNRSSIGSKFFENL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPQ5</accession>
    <name>D5GPQ5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00011975001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430377">
        <property type="protein sequence ID" value="CAZ86498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPQ5"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011975001"/>
    <dbReference type="HOGENOM" id="CLU_2086553_0_0_1"/>
    <dbReference type="InParanoid" id="D5GPQ5"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072240" evidence="3">
        <location>
            <begin position="22"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ86498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="117" mass="12508" checksum="9D748516E02F1A4D" modified="2010-06-15" version="1">MMPRIVSLLIFLLLQITTTIAHPLSKRKGGGGGRSGGGGGGGTGLSSGSLSWKTVLVIVGSILGTFLLLYIIFWQCVPGGRMFDWVKKVRDQRLEKKWAKYEVKDANASANEGQGGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPR6</accession>
    <name>D5GPR6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00011989001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the taxilin family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430377">
        <property type="protein sequence ID" value="CAZ86509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002842272.1"/>
    </dbReference>
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    <dbReference type="STRING" id="656061.D5GPR6"/>
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    <dbReference type="KEGG" id="tml:GSTUM_00011989001"/>
    <dbReference type="eggNOG" id="KOG1850">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="OMA" id="QTHFNTQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026183">
        <property type="entry name" value="Taxilin_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16127:SF13">
        <property type="entry name" value="GH01188P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16127">
        <property type="entry name" value="TAXILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09728">
        <property type="entry name" value="Taxilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="24"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="181"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="224"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="441"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="302"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="64"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
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            <begin position="181"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="441"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="451"/>
            <end position="464"/>
        </location>
    </feature>
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        <location>
            <begin position="465"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="525"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="572"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ86509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="584" mass="65119" checksum="4AE36DF064FC1CA6" modified="2010-06-15" version="1">MAAVANTSSVPSAQRYALLRASAGYGHPVSGPPTGAGGGAAGSGPSSFEVPVPSTDDYGPVSEPTSSNATPPLPTTAPAGSAPGSVPPNKKAPNTGPKSKKPLDPMEVNNLLTAKISQLETDASLEEEEEKAISRAVRRANKELAELVSSREDHLAKVDAIQNKYSELLYEMKRLERDYAKSRKRADQLQKEKDHSRSEMQKAVSMKHKLETLCRELQKENKRIKDESKKLAQNEQQKREELSNKFENTITEIKNRMEEENDGGSRKVNAGVDELFKAKFKSFVDQYEMRELHCYSLIRTKELEVQWNLARYEQQRKQAEQEAHRAKALHAQVSTFSQTEAELRSQLNIYVEKFKQVEDTLNNSNDLFLTFRKEMEEMSKKTKRLEKENLTLSRKSDTTNRNLLKMAEERTKHQKELEILRKKNSKLENLCRALQAERTTLDTKLRDQEGGGRGGSVGGAGEGGELLEEEEEEEDDSEVDSEYEESDRYDDDASEDGSEGEEGEDDTEDENPQAAETAVSPKVSGGNQKQQQHTQKNGVEKAYPVANGVSFGRPAHTTDLISLQLPPPSTSERTEKATCAGVER</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPU3</accession>
    <name>D5GPU3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00012035001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat MET30/SCONB/SCON-2 family.</text>
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        <property type="nucleotide sequence ID" value="XM_002842299.1"/>
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    <dbReference type="eggNOG" id="KOG0274">
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    <dbReference type="InParanoid" id="D5GPU3"/>
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    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0010992">
        <property type="term" value="P:ubiquitin recycling"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="CDD" id="cd22147">
        <property type="entry name" value="F-box_SpPof1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
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        <property type="entry name" value="WD40_repeat_CS"/>
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        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19849:SF1">
        <property type="entry name" value="F-BOX_WD REPEAT-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19849">
        <property type="entry name" value="PHOSPHOLIPASE A-2-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="F-box" evidence="6">
        <location>
            <begin position="476"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="718"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="792"/>
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        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="842"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="882"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="924"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="964"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="161"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="570"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1056"/>
            <end position="1077"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="162"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="221"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="580"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1067"/>
            <end position="1077"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ86536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1077" mass="119616" checksum="EB39342148B6ECA9" modified="2010-06-15" version="1">MEPPTAPHVNLPPRRHRSQQQIRGMDISTSASSGSMSQPSHTRTHRENRLAPYSTIGQFSLAPTTQTTVVTTTTTTTTSFPQLLMRPPRNLNELDPKEFPLAAVPTPSNLKRFCFDLNGKPTHFRETDDPEQSLRELDMLVHDLDRSNGLIRKVVRGEERVQVQNPLTSPPASATLPTASHSSSSRKRPASPVSISEAAERVSLLQKGNKRRQTGRPRSGIPSSKANTPSPRKCLRSSRNTTPATPVEINPPTTHAPRPVRPVHQQLSQLQNSQLFTTVNLTPNQNHQQETPLTLSPQLGVKPLDLEAEDLEMGSTPTPQEDLELDDAESDSTIIPTTLSPDTQHAANDNVAVGSAQPLSLPSPSLSPVTAALTHGGYFSAMDTDEIDSPLELPPSEEDVTDESNQLMLSPQRHIMQPQTPREQYTPGLMEIPKMLDSFDAMPSAVKTYVMYQLLRRCSKSTLQTVAEVVNPALKCDFLTLLPPELSLNIIKYLDIRSLCNAAQVSKRWRIIIDSDEWTWKKLFDNDGYVLGDGELDRAIREGWGFQDPQGDDDCERDVNAGTRSNCCAPPTLTTPPPHGRQKKKFNTRANSKKQQKKREAVETLGKEEFNEVILEIYSNSVGPVAAANAAEKAGSKALVGLQSLRNLHLFKSIYRRHHIIRRSWIRPQVKPRHISFRGHQRHVVTCLQFDTDKILTGSDDTNINVYDTNTGALRNKLVGHEGGVWALQYEGNTLVSGSTDRTVRIWNIAAGECTQIFHGHTSTVRCLQILMPTKIGVKPDGKDLIMPKVPLIITGSRDSTLRVWKLPMRGDKPYLPGASRDANDNDLFLNPNNNPYFIRTLSGHQHSVRAISAHGDTLVSGSYDYSVKVWKISTGETLHTLRGHVQKVYSVVLDHKRNRCISGSMDNLVKVWSLETGSVIYTLEGHTQLVGLLDLSHDRLVSAAADSTLRIWDPETGNCKHTLSAHTGAITCFQHDGHKVISGSDRTLKMWNVQTGEFIRDLLTDLSGVWQVKFNERICVAAVQRDQVTYIEVLDFGAARDGIPDDKRGRRIVVDRSGREIPDGNDTMDEAGDDFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPU6</accession>
    <name>D5GPU6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00012038001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SAPS family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430378">
        <property type="protein sequence ID" value="CAZ86539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842348.1">
        <property type="nucleotide sequence ID" value="XM_002842302.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPU6"/>
    <dbReference type="FunCoup" id="D5GPU6">
        <property type="interactions" value="950"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GPU6"/>
    <dbReference type="GeneID" id="9182283"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012038001"/>
    <dbReference type="eggNOG" id="KOG2073">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003676_2_0_1"/>
    <dbReference type="InParanoid" id="D5GPU6"/>
    <dbReference type="OMA" id="ECKSHNP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008287">
        <property type="term" value="C:protein serine/threonine phosphatase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072542">
        <property type="term" value="F:protein phosphatase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019903">
        <property type="term" value="F:protein phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905560">
        <property type="term" value="P:negative regulation of kinetochore assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007587">
        <property type="entry name" value="SAPS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12634:SF8">
        <property type="entry name" value="FIERY MOUNTAIN, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12634">
        <property type="entry name" value="SIT4 YEAST -ASSOCIATING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04499">
        <property type="entry name" value="SAPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="409"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="675"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="723"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="853"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="432"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="685"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="731"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="747"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="760"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="782"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="862"/>
            <end position="872"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ86539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="891" mass="97598" checksum="865D7D07F75136C8" modified="2010-06-15" version="1">MFWRFGGYANISTLDAILEKPDVTLEDLLEESDLIQELKQQNAKLIEFLRREDVMWKLLEYVVAPKRSDPASEEAGNNAAEAIRLKYAYVCCEVLSSESWSMAETLVENLQPLRMFWSFLERPAPLDPLQAGYFTKVNETLLDRKTDEMMEFFKGLDNIVPKILKHVDCPMIMDLLLKIISMEKCEGGTGIVDWLHSQNLIPLLLSFLSSDHPATTQTSSGDFIKAIITISANASQNEQSCIGPNDLTRQLVSEACVQGLIRDMLNGGNPLTVGVGIVIEVIRKNNSDYDPDVGTGVDNIPSSRDPIYLGTLLRLFAKHVPDFMKLILTQCMVVTSEDGSKTEKQRELKAAFGETIEPLGFDRFKTCELMAELLHCSNMGLLNERGGESLLKGGRSLVDDLKTASIAAPLNPQASSIPQDRMDADLPPLPKEAFEKSKQPDDPEEDLLDLGGGSPYSTATFRSTGNSSFEEYETMIEKDFEGNPVVGDYLKMMFVENSVVPTILDFFFRFPWNNFLHNVVYDVVQQVFNGPMERGYNRALAIDLFATGRITERIVEGQKASDKVEAEKKMRLGYMGHLTLIAEEVVKFAERHPPEILGHKVIEKVSDPLWIEYVEETLAATRERDNAILGGVRPDMSVGPRQAVLNAVQAQAFNANGSSGSAGIGSGAGISGSSGGLLSGFGSSSDEEDEDMERDDEDDDEVNRLGLSGSSDQFASYISQEITNVDKFGSSDEEDDDDDISFATAPNEPPPPPPLPLNITRPSSATTATNTMSVGANAGIEIEGEEGGFEEIEEVGDFAGDGDSSDEGDDELISRGVLRRNKLTVTHKAEKRAVLEEDEGEVDFINEIHGLSLAGVGDQGKATSETSASVTTEAERISEPAAGEAQKIETK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPU8</accession>
    <name>D5GPU8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00012040001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="FN430378">
        <property type="protein sequence ID" value="CAZ86541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842350.1">
        <property type="nucleotide sequence ID" value="XM_002842304.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPU8"/>
    <dbReference type="STRING" id="656061.D5GPU8"/>
    <dbReference type="GeneID" id="9182285"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012040001"/>
    <dbReference type="eggNOG" id="ENOG502S24K">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041456_2_1_1"/>
    <dbReference type="InParanoid" id="D5GPU8"/>
    <dbReference type="OMA" id="QQRMLVH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004656">
        <property type="term" value="F:procollagen-proline 4-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045054">
        <property type="entry name" value="P4HA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869:SF241">
        <property type="entry name" value="FE2OG DIOXYGENASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="7">
        <location>
            <begin position="121"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="233"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="234"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ86541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="258" mass="29195" checksum="B4846B27E56CCC64" modified="2010-06-15" version="1">MAAMAPPTATRIDFNQTRLDEVYGNPNNCFAMIIENAFTPTECEELIALAESTGSWMPAAKNVQVRDAMRILRLDAETARVVFERVEPFLDEVRRPGKELESMIGKKGGGSGEKGRWELAGLNEMLRFLRYEEGQHFNLHCDATYNGGGGEKRQKSFLTLHLYLTDDESSSLKGGSTRFWSPDEAKFMDVEAKRGRVLVFQQRTLWHSGEPVTEGTKITMRSDIMFEWVSLKPPRPKGEKKKGGRVDKRVESVKQTMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPZ0</accession>
    <name>D5GPZ0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00012101001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430380">
        <property type="protein sequence ID" value="CAZ86583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842392.1">
        <property type="nucleotide sequence ID" value="XM_002842346.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPZ0"/>
    <dbReference type="STRING" id="656061.D5GPZ0"/>
    <dbReference type="GeneID" id="9185185"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012101001"/>
    <dbReference type="eggNOG" id="KOG0116">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022209_2_0_1"/>
    <dbReference type="InParanoid" id="D5GPZ0"/>
    <dbReference type="OMA" id="RPRGNAY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990861">
        <property type="term" value="C:Ubp3-Bre5 deubiquitination complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034517">
        <property type="term" value="P:ribophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00780">
        <property type="entry name" value="NTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.50:FF:000003">
        <property type="entry name" value="Nuclear transport factor 2 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002075">
        <property type="entry name" value="NTF2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018222">
        <property type="entry name" value="Nuclear_transport_factor_2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039539">
        <property type="entry name" value="Ras_GTPase_bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10693:SF20">
        <property type="entry name" value="AT27578P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10693">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEIN-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02136">
        <property type="entry name" value="NTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50177">
        <property type="entry name" value="NTF2_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="NTF2" evidence="5">
        <location>
            <begin position="38"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="412"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="207"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="482"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="17"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="249"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="276"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="289"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="308"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="342"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="525"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ86583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="559" mass="59442" checksum="639E2DAF7729240E" modified="2010-06-15" version="1">MASPQPPAAPTNGGYQGQATPQHSENNTGNSDVPKDEVGWYFVEQYYTTLNKTPERLHLFYNKTSSFVWGTEGENLQLAHGRSAIQDKITSYEFKDCKVRVSNVDAQSSADDGIVIQVLGEMSNNGLPNRKFSQTFFLAKQPNGYYVLNDIFRYLKDDEETEEGEYDEYNMAEGVAEDAEVTETITEEVIEAVEDVGLKETTTTEIEVQEDKVTIEETTTIEPALPIPGTQGLPPPINGGANGSSAEAPAEEPEAEVQPDPEPEPEPEAEEPAVPPVEAAPEESASPAIEEKKPAPEKEPERRAVTPVPVPPPVPAGPPKPTTWASIIAKSANTGAPAAVPQSVTPPQIQQAQQAQPSQAQNSTTPVTSTTSSSATPSTPTTPRGGSQWHTTESKRHSRPAPVSANAAISQTSAYVRNVTEGVTDRALEDALTKFGALERIEINRQKNCAFVEFETSAGFAAANASNPHKIGDANVYVDERRPRGAASGPGYSGGRGPFGNRDSRTGGQGGRGFSSREGGRSEGGRGGSRGGARGNYNQHRGGAAGRPGNMGAKDLESC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>D5GQ21</accession>
    <name>D5GQ21_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00012158001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430381">
        <property type="protein sequence ID" value="CAZ86614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842423.1">
        <property type="nucleotide sequence ID" value="XM_002842377.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GQ21"/>
    <dbReference type="GeneID" id="9185194"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012158001"/>
    <dbReference type="HOGENOM" id="CLU_883370_0_0_1"/>
    <dbReference type="InParanoid" id="D5GQ21"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="284"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="248"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ86614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="315" mass="33153" checksum="C48D583385B86BA3" modified="2010-06-15" version="1">MYYGCAAAAFEKPTYPGNASMVLRKYSMGVQYSRVPGGKTAGMTDGRARGGGHKTIKYFPHTATQEGEKTSSPFLPFPHPTNQLIATPTVMATLCAILTLLILTVLTPSTTASNILSNEVLKLPDYPYLTSCQQKCAAGEANNQASPTSIFSLTICETKACFCPAQNEPQIGRQAQNCLEGVSGGVCATAKEYNGLMAFVAKYCGFEYVPTTTGLSFAPSGSGATSTQGATATASCWDTNGYPVETEGCSSSMTTATRTSDPYPTSTSSGSPQETRTLLDGKNAFTIGIITGFSILGIVGCALLLRGHIRRRRAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GQ31</accession>
    <name>D5GQ31_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00012177001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430382">
        <property type="protein sequence ID" value="CAZ86624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842433.1">
        <property type="nucleotide sequence ID" value="XM_002842387.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GQ31"/>
    <dbReference type="STRING" id="656061.D5GQ31"/>
    <dbReference type="GeneID" id="9185204"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012177001"/>
    <dbReference type="eggNOG" id="ENOG502S6QC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_685161_0_0_1"/>
    <dbReference type="InParanoid" id="D5GQ31"/>
    <dbReference type="OMA" id="AKHSHLY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="3">
        <location>
            <begin position="140"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="212"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="14"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="29"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="65"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="77"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="218"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="282"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="341"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="393"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="407"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="435"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="450"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ86624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="460" mass="50544" checksum="217CD5F3884CC368" modified="2010-06-15" version="1">MSWNSPPNGRHPHQKEWEKTTEDKEQHPGRSNSPSQTESQLHYSEHHVNPIHPHQSQAQHPLPPHSHHSQHHHPHPHPPPPAQTPHLPPIQQERLAIEQPEMYAASEPPYPYRIPPHQLPGFQQPANFQQPAPRQRTAIACRYCRRRKIRCSGFESSPDGRCANCLRFNQECVFTPVSSQTQAFVPAHALYNTTRSVGGRAGPLYGAHGQPLSSGYIPMPSQAPAPSAQSPMESRAPQQYPRPPSPGVGSGRQPPPPPAQPPYGSYEELVTCEPERNRSQEPHPPLLPPPVPGNQPQPHHLQRRSSASEYQSHYARNDPPSPSSHLLHAQHAYSPPPVSGPPSSSFQASSYGQGPPLSAASNYYPPGPGASQQRTRSPVGFRPPDRSSGSPHPFHPPMQYPPPPRPTSSGPSPSSPNTHPIPSMQMANLIDPSQPHGQGRNQRTAADGDMLDRLMPRSGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>D5G455</accession>
    <name>D5G455_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00003957001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835177.1">
        <property type="nucleotide sequence ID" value="XM_002835131.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G455"/>
    <dbReference type="STRING" id="656061.D5G455"/>
    <dbReference type="GeneID" id="9182425"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003957001"/>
    <dbReference type="eggNOG" id="KOG2625">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027041_1_1_1"/>
    <dbReference type="InParanoid" id="D5G455"/>
    <dbReference type="OMA" id="YLCVHNG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990072">
        <property type="term" value="C:TRAPPIII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010378">
        <property type="entry name" value="TRAPPC13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055429">
        <property type="entry name" value="TRAPPC13_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055427">
        <property type="entry name" value="TRAPPC13_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13134">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13134:SF3">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23647">
        <property type="entry name" value="TRAPPC13_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06159">
        <property type="entry name" value="TRAPPC13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Trafficking protein particle complex subunit 13 N-terminal" evidence="2">
        <location>
            <begin position="14"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="Trafficking protein particle complex subunit 13 middle" evidence="3">
        <location>
            <begin position="193"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ79298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="319" mass="35047" checksum="4BF3811EE50C72F1" modified="2010-06-15" version="1">MPRERAVSEVKEPHSISLKVLRLSRPSLSEQHSLPKATPSNQSPELDELSRQSHAYPSHSTDDPFILSPLLTLPPAFGNAYIGETFSCCLSANNETTSITTSVRISAEMQTPSLTLNLELGGDERQTADLDPVMSLQKIVKYDLKEEGNHILAVTVTYTEAPKRVDYGEGEKGAPGRVRTFRKLYQFIAQQCLTVRTKIGSLSGGRAILEAQLENMGDGPISLEMVHMGTTKGWTATSLNWQGSTGRGDGQRNPKDTPMLGSRDVMQVAFLLYPEETEEGWEEDVAANDKKILGQLSIEWRSACGDRGYLSTGRLGLHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>D5G495</accession>
    <name>D5G495_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00004010001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835217.1">
        <property type="nucleotide sequence ID" value="XM_002835171.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G495"/>
    <dbReference type="GeneID" id="9184963"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004010001"/>
    <dbReference type="eggNOG" id="ENOG502SZ5R">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_984158_0_0_1"/>
    <dbReference type="InParanoid" id="D5G495"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="4"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="241"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="262"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ79338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="283" mass="31533" checksum="547BEA32B3EC512F" modified="2010-06-15" version="1">MVIGLSLEKLPLEVIHQVFSLLSPQDLTSLRLTSRSILPAATANTHWRHHFFDGLLSSHAALIDISSIARDQWAFQLVPDGWFRAYTTLIRIANEDWLRTEEELKGSTWTVFFKMYLREGGASSDDETGHALVELTHDLTSNRIEGLPLRSSSWYVDQKKTLRFSGYPPIVPSRDPHTWYALNVLNTAILEESNTAGLIDEDKRQALESGVHDSLRAAQEKRKNELPARLRELHLLRSSARERSMNCGYERPSDLGSSKSGGASGSGNGGIAVGPVEEAGKRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4R1</accession>
    <name>D5G4R1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">polynucleotide adenylyltransferase</fullName>
            <ecNumber evidence="4">2.7.7.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00000060001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DNA polymerase type-B-like family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.19" evidence="4"/>
    <dbReference type="EMBL" id="FN429990">
        <property type="protein sequence ID" value="CAZ79497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002835294.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4R1"/>
    <dbReference type="GeneID" id="9188433"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000060001"/>
    <dbReference type="eggNOG" id="KOG2277">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002806_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4R1"/>
    <dbReference type="OMA" id="TENHLCM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990817">
        <property type="term" value="F:poly(A) RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010605">
        <property type="term" value="P:negative regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05402">
        <property type="entry name" value="NT_PAP_TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054708">
        <property type="entry name" value="MTPAP-like_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002058">
        <property type="entry name" value="PAP_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12271">
        <property type="entry name" value="POLY A POLYMERASE CID PAP -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12271:SF113">
        <property type="entry name" value="POLY(A) RNA POLYMERASE CID11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22600">
        <property type="entry name" value="MTPAP-like_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03828">
        <property type="entry name" value="PAP_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81631">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Poly(A) RNA polymerase mitochondrial-like central palm" evidence="10">
        <location>
            <begin position="125"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="PAP-associated" evidence="9">
        <location>
            <begin position="346"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="462"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="554"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="621"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="788"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="833"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="23"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="47"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="488"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="554"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="848"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="908"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="989"/>
            <end position="1007"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF22600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ79497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1007" mass="109967" checksum="F93FE2422836B7F2" modified="2010-06-15" version="1">MAGRRNESGPPMTLSHARPPFKNQRASSSAQSNSLPSTPNHHPRQANGASRSPSPTSALDSPRSAASEPLIPSIQKAPVVSPRTCIYETGLANIRRRMPYSLGIDKLGNEKPKAERLSVEQEGKFTEDIKSLFETLKPSEASGDRRRRFLEKLERLLNREWPGHDIQVHAFGSTENHLCMIDSDIDVCIKTSWDGLKSTCYLAARLAKCGMERVVCVPGAKVPIVKIWDPEYQVACDMNVNSTLALDNTKMIKTYVEIDERVRPLAMIIKHWTKKRVLNDAAGGGTLSSYTWICMILNFLQTRDPPILPALHQRPHKKRPPINGVDISFDDDIETLKGFGHNNKETLGELLFAFFKKYGHELDYEKRVISVRHGKLLSKEEKNWQYLQNNRLCVEEPFNFTRNLGNTADDSSVRGLHLEFRRAHKILSEDADLAAVCEQYEFPPGEPHTSVPIQQPVHRPVTLTRGYPNHKSRGGYNNRGNRPQHYMNGNRNGNRKPGNNNYNNHSNNVPLQHRYLSPEIFGYVQTPQEQLLALQAQFHHARVAQAQLAQAHVHAQLHGQQTQTQSSSASTNAHGSALTPQDFAAMGTLSSYAYFAQLWGMNYYYPSAPLHSELPTQMAPQVATGMNESRQRAGQRRHNGYSAGSRSQSQPPPLSEVYSVPSYGRGGSSATGVTGSEGDDFADHSSNGNPATPPEEEPDEYVGYYAYGGSLQSAPAVVDSVGEEEEPFIEQKSIVDRQSISLGSPGSFEDRGPVIVNGSISTSSVPYPDTPYSGLPLPSEELYQYDGLMSGVDPGGSERSGDKSLGTEAIKNDTSSLAQKLLEVHIQAAVGGPEAGLTGSAQTYTNGPSSASVSTSSLLSSPQNTQPSQVTSDDDVDTSVSPRLSPNLRQRAAREQLLWSNARVPPADNTNSKTAGASQADLAPPLTPVPEVRSPSPTPAPISVPAPVLAPAKRESSSTLPPKSGHNGHSGRRSGRNHSAQVPPTPADKSSKSSVAEDQKEELTQNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4V5</accession>
    <name>D5G4V5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00000107001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429992">
        <property type="protein sequence ID" value="CAZ79548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="tml:GSTUM_00000107001"/>
    <dbReference type="HOGENOM" id="CLU_2741883_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4V5"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ79548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="71" mass="7952" checksum="286C779AE228F3C1" modified="2010-06-15" version="1">MFLYSCECTNKESIISNCGRDQPHSDCTKVAVPNIKISLGSLFQGFGRKVSLYRHPSITSTKYRDCPTSFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5G536</accession>
    <name>D5G536_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00000236001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429996">
        <property type="protein sequence ID" value="CAZ79621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835464.1">
        <property type="nucleotide sequence ID" value="XM_002835418.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G536"/>
    <dbReference type="FunCoup" id="D5G536">
        <property type="interactions" value="87"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G536"/>
    <dbReference type="GeneID" id="9187111"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000236001"/>
    <dbReference type="eggNOG" id="ENOG502SDMM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102617_2_0_1"/>
    <dbReference type="InParanoid" id="D5G536"/>
    <dbReference type="OMA" id="RSHDNHA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007250">
        <property type="entry name" value="HSP9_HSP12"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04119">
        <property type="entry name" value="HSP9_HSP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002590">
        <property type="entry name" value="HSP9/HSP12_fun"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="87" mass="9762" checksum="D077E30824DBEDB3" modified="2010-06-15" version="1">MSEPFRKDFSTQAREEITPDSSKSTLDRAKECVTGTADRAAGGLQSDHHKSNTQEAFDRTRREKDSQKDNSESLLDKTKHALGVDKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5G596</accession>
    <name>D5G596_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00004235001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835532.1">
        <property type="nucleotide sequence ID" value="XM_002835486.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G596"/>
    <dbReference type="GeneID" id="9188108"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004235001"/>
    <dbReference type="eggNOG" id="KOG1890">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006249_1_0_1"/>
    <dbReference type="InParanoid" id="D5G596"/>
    <dbReference type="OMA" id="YFWNRHL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043812">
        <property type="term" value="F:phosphatidylinositol-4-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034753">
        <property type="entry name" value="hSac2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022158">
        <property type="entry name" value="Inositol_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002013">
        <property type="entry name" value="SAC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45662">
        <property type="entry name" value="PHOSPHATIDYLINOSITIDE PHOSPHATASE SAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45662:SF7">
        <property type="entry name" value="SACI DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_1G15890)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12456">
        <property type="entry name" value="hSac2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02383">
        <property type="entry name" value="Syja_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51791">
        <property type="entry name" value="HSAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50275">
        <property type="entry name" value="SAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAC" evidence="2">
        <location>
            <begin position="276"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="domain" description="HSac2" evidence="3">
        <location>
            <begin position="703"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="217"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="356"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="219"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="367"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ79689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="943" mass="105046" checksum="584AC45E8AC1D7AA" modified="2010-06-15" version="1">MPPLARKILVTAAIDGLILSPIQSSRNVRPPPLHGSVRIDYKSKLIFSYNPTEESQAAEALSTLSSSIEAHGIVAGLLNVSPASAFLIAVTDRERVATIRGRYPIYVVTDVALIPLSSRKDSEAAIFKARAWKSGAGEVESDEAPDTDIEEDDDTAIVDDELQDQVNPDSKSVNHRGQSSIGEDVIGRRGAYGRFAENWFSKRGWTVDRIRAEGLSSDEGIREEDPKAATEDIASASDEEIKDTKRPESQQVEEDAEAMKEDVAHSLTPKLLRTTRILLASSRSFFFSYDWDITRAWGRDEENQSSSLPLRESVDSLFFWNRALQKPFIESGNDTFVLPIMQGFVGQFQFSATVPSGPELWPPTEPGEQKPTEVSHEDGKQNLLLTLISRRSIKRAGLRYLRRGVDDCGNVANCVETEQILSDPDWNRVFSHVQLRGSIPLYFQQSPYALRPKPVLLRSEAANAEAFQLHFKNIKQRYGSIHAVSLVEKRGNEAIIGGKFQSSFENLLNANKGVGFNWFDFHRECRGMRFENVKLLFDEIGETLDKFEYTEEIQEDGKSKRVKSQSGVLRTNCMDCLDRTNVVQSFCARNSLDKQLISLGVTSMSAEEEMKVSAGFNILWADNGDAISKQYASTAALKGDFTRTRKRNYRGALTDFGLTLSRFFTNVVSDFFTQAAIDFLLGNVTSKVFEEFEEEMITADPAVSVKKVRENAVETSAKIVVEDENEVVLGGWTFLTPSGEDRGHKPPLREAVILVTDRALYRCRFEFGIEKVREFERVELEDIIGVQWGTYIISTLTPSSTDGRRNIGFLLKYRPPRTGELVRINTRSLRSTFAKPMGEYHDHDDSDTHSEAAAPFTAAAATISSSSATRIMAFKAPASGESGEVDLVKSVCQEIANACAKNRPAAAMEAFVENKDVIGLAEAKKSTGLLEQWGYNLRKLVWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5C3</accession>
    <name>D5G5C3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00004267001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835559.1">
        <property type="nucleotide sequence ID" value="XM_002835513.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5C3"/>
    <dbReference type="FunCoup" id="D5G5C3">
        <property type="interactions" value="878"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G5C3"/>
    <dbReference type="GeneID" id="9188135"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004267001"/>
    <dbReference type="eggNOG" id="KOG2048">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002392_2_1_1"/>
    <dbReference type="InParanoid" id="D5G5C3"/>
    <dbReference type="OMA" id="MWDVELP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034455">
        <property type="term" value="C:t-UTP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046351">
        <property type="entry name" value="UTP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44163">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44163:SF1">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="199"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="483"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="561"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="791"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="489"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ79716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="910" mass="100445" checksum="7F349DC9B0827829" modified="2010-06-15" version="1">MTHVSLHRCRFVDFPPSQITALAFSHQSTPASPHPPPKNLRLAIGRANGSIEIWNPLLGAWVYESTLAGGKDRSIEDLTWVQDYDSHGRGLLRLFSIGYSSVVTEWDLVTGRPRTHTDCNGGVIWSIAAQPRLHSSDKVDQENQEACSQKIVVGMQDGALTILSTAGGKGALSYVKTLMRAGTGKSRVLSMAWQNRHTVAAGMADSTIRVWDIRSGRVVSRLSLNKGRGRDVLVWAVRALPNGDLVSADSRGEVCFWDGDNYTLKQRIKGHDGDCLTLEVGGINGDTVISGGVDMKTILYKYIGKGRKWAQVTLRRFHKHDVRAMAAYECGKFSVIASGGVDMTPIIIPLRQFLDESHLTIPAVPQSPIVTSVPESRLIMSWWEQEIKIWRIQELGYDENKGDELYLFPGEEEGRGRRLLSRIVLANDEYITSASISSPIPQNGGHLLAVSTIAEVKLFLLRPPKSSSGSASLRVSKITIPDSLHLEAPEDEDDSDDEMEEDVDLANEGARLIRISPDRQWLSIVTPDSRVLIAKLAVEPPASPKSKATVTFESSVFELPRQGRPETAFRAPPPPTADTPSKRQPKRLVHRIDEGDLEAYPRTINHHLFSSTSNLLAVSDLSGYLDTYILTNSTWKLNPKNPLLPRLNSSLSTLTFRPSFPSPTTSTITSTSNPEDRLILITSKENHILELHLTTGALSDWSRRNPPTYLPLDFRTLKSHATGTFFETSQWKAKQRLWVWSADWLWMFDLLKDLPEVQPRAEATRGKLGSGAGHKRVMTGGLVASGPIVNPPLPAPAKTNTGGAVTDSEWEGGGYEDGGASRAIVLASTHKKRSKSGVKSAFWSSNRYKSMLGFLPVGYREEILIKGEGWRDGQVLEAMEMVIIERPMWDVKMPPRFSKDSGNLARVGGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5G0</accession>
    <name>D5G5G0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00004319001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835596.1">
        <property type="nucleotide sequence ID" value="XM_002835550.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5G0"/>
    <dbReference type="STRING" id="656061.D5G5G0"/>
    <dbReference type="GeneID" id="9183739"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004319001"/>
    <dbReference type="eggNOG" id="ENOG502S8J5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_874275_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5G0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="138"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ79753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="330" mass="36165" checksum="DC1EEC0BFBCE3A5A" modified="2010-06-15" version="1">MSSLASSIIKELINFTIPLVHSFILLLALLLVLGAIVVYIYEQTNKRRLQVVPETQPVAKEPLLVEAEQALIGEAEEGYGGDSDKVEEKHPQDTETGGLMRQWQVQNPDFVVPPELEEGAPANGAGLPQFNAGGGDGTTPAAAAGPASGQTRPHIPHTRNVGAKKARSLARRDQRRAYHELLHSQAAMRASATAERDEMFEEKRRCALVEDEIAAKKEAETTTWLEAERNGAENERGDVEGLKKKVAAPGAWRVSALGGKRGEEWGVEVLRREGLVGKVRGGEDVGIITGEGWYVRLGERQLESISGEIEKKGRMEWREIADRLEWVVGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5I7</accession>
    <name>D5G5I7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00004355001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GILT family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835623.1">
        <property type="nucleotide sequence ID" value="XM_002835577.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5I7"/>
    <dbReference type="GeneID" id="9183766"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004355001"/>
    <dbReference type="eggNOG" id="ENOG502S45F">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072148_1_0_1"/>
    <dbReference type="InParanoid" id="D5G5I7"/>
    <dbReference type="OMA" id="GPTECIG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016671">
        <property type="term" value="F:oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004911">
        <property type="entry name" value="Interferon-induced_GILT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13234">
        <property type="entry name" value="GAMMA-INTERFERON INDUCIBLE LYSOSOMAL THIOL REDUCTASE GILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13234:SF8">
        <property type="entry name" value="GAMMA-INTERFERON-INDUCIBLE LYSOSOMAL THIOL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03227">
        <property type="entry name" value="GILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003071990" evidence="6">
        <location>
            <begin position="24"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ79780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="276" mass="30603" checksum="C85C1490ADA4BC90" modified="2010-06-15" version="1">MSSSRVAIPFILVVVILYTSYIATPDPGALASFLTRDYPPNHNVDNQQSLRAPGLAAEIKVPLEIHIMSKCPDARDCLEQLIAPTLEEIESLVDFQMSFIGRPTADDGVFCMHGPSECLGNILHLCSAKINTPPSPPPAHTYLPFSLCLIHDFKNIPEREFVEGCADKNGIDFQKLNDCASDMGPDGGLSMLRNSVERSRALEAETSCTVRVDGKKRCVRDGGKWKDCDGGSDIEDLVRDIKKIYKEKNGGHERFGEKSKKLSEWIESERIIWTGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5K0</accession>
    <name>D5G5K0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00004378001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835636.1">
        <property type="nucleotide sequence ID" value="XM_002835590.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5K0"/>
    <dbReference type="GeneID" id="9183779"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004378001"/>
    <dbReference type="eggNOG" id="ENOG502S621">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_794468_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5K0"/>
    <dbReference type="OMA" id="RVHRTCH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="183"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="280"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="337"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="48"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="195"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="339"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="376" mass="42192" checksum="41ADF6BD714B49AA" modified="2010-06-15" version="1">MSTGTPTGGSGSAGGGERSIRKRFASQVKRVLSIKNKPGRSRRSTVSDGPGEPDTPTTPLLPTTAVPPIESKPSSVARPGPEPKAQEEVEQQRVKTRTRPAVRQPRTTNIPPPAVSAAERAQALFKKHGLEVSTADWPLSTVLPSDRVQKDIRMRVHRTCHKCSTSFGPDKICNSCNHKRCKKCPRQPVKKPKDKGKGKEKVGERSGQVVGRKKKKKPTYGVTLTIPSRTGGQDLVRKAPRQRVHRKCHRCQTDFAGEKICRKCSHTRCRSCPREPFKKNKPPGYYDSLDPEDSEEERQLPTRPRRTYKKPRRRIHWICTKCSTTFVERTKICEGCGSNRDDTGLRDPPKKQPYKPTEEDIQRLDERLKQTSLSAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5K8</accession>
    <name>D5G5K8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Probable quinone oxidoreductase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">NADPH:quinone reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00004387001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835644.1">
        <property type="nucleotide sequence ID" value="XM_002835598.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5K8"/>
    <dbReference type="FunCoup" id="D5G5K8">
        <property type="interactions" value="610"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G5K8"/>
    <dbReference type="GeneID" id="9183787"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004387001"/>
    <dbReference type="eggNOG" id="KOG1197">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_3_1_1"/>
    <dbReference type="InParanoid" id="D5G5K8"/>
    <dbReference type="OMA" id="VDMSYSR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032440">
        <property type="term" value="F:2-alkenal reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035925">
        <property type="term" value="F:mRNA 3'-UTR AU-rich region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070402">
        <property type="term" value="F:NADPH binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003960">
        <property type="term" value="F:quinone reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05286">
        <property type="entry name" value="QOR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000053">
        <property type="entry name" value="Quinone oxidoreductase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047618">
        <property type="entry name" value="QOR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002364">
        <property type="entry name" value="Quin_OxRdtase/zeta-crystal_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48106">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE PIG3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48106:SF13">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01162">
        <property type="entry name" value="QOR_ZETA_CRYSTAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="5">
        <location>
            <begin position="17"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00070796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ79801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="331" mass="35488" checksum="4BCECA452C899716" modified="2010-06-15" version="1">MSSLPKTQRSVRINAHGGPDVIEIVTDAPVPTPTDNEVLVKNTYAGLNFIDIYFRKGVYPTSSFPLTLGREAEGTIAAVGKSVQNFEVGDKVAYLGVGAQQEYTALDPLHVAHVPSGIPEGVAAAGILQGITALTFARESYEIKKGDYVLIHAAAGGFGLWLVQIARVIGAHVIATASSDQKLAIAQEHGAEFLINYSTQDWVAEVKEITKDAGGGVAAVFDGVGKDTFDGDLEVVRRKGTVVSLGNASGAVPPFTIARLAPKNLKLLRPQLFGYIAERGEFEAYSKEVFDWIVEGKVKVNVHKTYPLEEVAQAQKDLESRTTTGKLILKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5U8</accession>
    <name>D5G5U8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00001584001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430002">
        <property type="protein sequence ID" value="CAZ79891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835734.1">
        <property type="nucleotide sequence ID" value="XM_002835688.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5U8"/>
    <dbReference type="GeneID" id="9187569"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001584001"/>
    <dbReference type="HOGENOM" id="CLU_1205535_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5U8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="230" mass="25576" checksum="1D504EB28CF92628" modified="2010-06-15" version="1">MQATTPQACNQFLAHKSAASYPKRHLYTGFACGIADPDWEFIDREYSNSCHETGAALAYKFASEENPMQLRNDTGGMDDDEFNDEDEGVDVVYPYQQYMNKAATVASEEEMSDVPECEMSSSHEGSVIDDDVLDRMDAVANSGFGTGAFSHCPVVLTSDGGDEVMEIEMGIQMDDISVDSSKKRKRWADDDDNAQQQIHQLQYPTQTKFAGAPWGALQQNNAKRDQARRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5Y4</accession>
    <name>D5G5Y4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transcriptional activator HAP2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00001625001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the NFYA/HAP2 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430002">
        <property type="protein sequence ID" value="CAZ79927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835770.1">
        <property type="nucleotide sequence ID" value="XM_002835724.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5Y4"/>
    <dbReference type="STRING" id="656061.D5G5Y4"/>
    <dbReference type="GeneID" id="9187605"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001625001"/>
    <dbReference type="eggNOG" id="KOG1561">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045001_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5Y4"/>
    <dbReference type="OMA" id="PRSHIPN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001289">
        <property type="entry name" value="NFYA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12632">
        <property type="entry name" value="TRANSCRIPTION FACTOR NF-Y ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02045">
        <property type="entry name" value="CBFB_NFYA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00616">
        <property type="entry name" value="CCAATSUBUNTB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00521">
        <property type="entry name" value="CBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51152">
        <property type="entry name" value="NFYA_HAP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="75"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="256"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="44"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="80"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="113"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="183"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="307"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="372"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="395"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ79927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="406" mass="43634" checksum="889AD0500B3FFC00" modified="2010-06-15" version="1">MAFAPRYPQPHSTHHVHGTAGSYSPQQNPGGGVSGGSPHIPNASMHQPGNQHSPQMPTTSHQYYTPYMTTAIPQPGPFFMQSSDNGLPLSGQKSIASPRMNTGPTKVKREGTQNRSPQMASSTTNQVGSGQQVIQPPNQQVSQQTMQNVAQLSQRRMSQQQGPTGSPSLQHAQPVNSTRTSAPPQPPATQQTAPPPPPQSQPPPSQPVQQPPMHPPVQPHQQSPDLGPPEESPLYVNAKQFHRILKRRVARQKLEEALRLTSKQRKPYLHESRHNHAMRRPRGPGGRFLTAEEVADMEKKQREQSGNGAGDSNGGNNGQPAAVTKNKEADKANATRTPNRMPPNAAHPHSGGGSSAPTSGSMKRKAGQAGLPSSSPVKKAKSSNPRSHIPNINSESDDEELGEEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5G607</accession>
    <name>D5G607_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00001714001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the XPG/RAD2 endonuclease family. XPG subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430005">
        <property type="protein sequence ID" value="CAZ79950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835793.1">
        <property type="nucleotide sequence ID" value="XM_002835747.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G607"/>
    <dbReference type="STRING" id="656061.D5G607"/>
    <dbReference type="GeneID" id="9187628"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001714001"/>
    <dbReference type="eggNOG" id="KOG2520">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1367120_0_0_1"/>
    <dbReference type="InParanoid" id="D5G607"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004520">
        <property type="term" value="F:DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09868">
        <property type="entry name" value="PIN_XPG_RAD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000061">
        <property type="entry name" value="Single-stranded DNA endonuclease (Eurofung)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006084">
        <property type="entry name" value="XPG/Rad2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001044">
        <property type="entry name" value="XPG/Rad2_eukaryotes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019974">
        <property type="entry name" value="XPG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006085">
        <property type="entry name" value="XPG_DNA_repair_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16171">
        <property type="entry name" value="DNA REPAIR PROTEIN COMPLEMENTING XP-G CELLS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16171:SF7">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00752">
        <property type="entry name" value="XPG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00853">
        <property type="entry name" value="XPGRADSUPER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00066">
        <property type="entry name" value="XRODRMPGMNTG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00485">
        <property type="entry name" value="XPGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00841">
        <property type="entry name" value="XPG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XPG N-terminal" evidence="7">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ79950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="200" mass="22803" checksum="2FA78B3B537E490F" modified="2010-06-15" version="1">MGVTGLWTIANPCARPVKLESLARKRLAVDASIWIYQFLKAVRDKEGHALQSSHVVGFFRRICKLLFFGIKPVFVFDGGAPPLKRQTIAGRKARREGRKEDARRTAGRILALQVQRSADIEAARRRNRNLKGRRGLGTRLRMTRESCRRMIWKSMPVCSVAGRKSTFTTFPRSTLTRISLSRYPWRIGTISSMLRDYVPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5G691</accession>
    <name>D5G691_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00001660001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430008">
        <property type="protein sequence ID" value="CAZ80034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835877.1">
        <property type="nucleotide sequence ID" value="XM_002835831.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G691"/>
    <dbReference type="GeneID" id="9184306"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001660001"/>
    <dbReference type="eggNOG" id="ENOG502S5S4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040566_4_0_1"/>
    <dbReference type="InParanoid" id="D5G691"/>
    <dbReference type="OMA" id="WLIDNAT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00413">
        <property type="entry name" value="Glyco_hydrolase_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38121">
        <property type="entry name" value="GH16 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00722">
        <property type="entry name" value="Glyco_hydro_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="280"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="2">
        <location>
            <begin position="3"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ80034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="300" mass="33429" checksum="A6CEA185D99772FC" modified="2010-06-15" version="1">MHTDFSKIPDTTDVNTALSDWEVQDWAFNTSSSAGVMARKNDAENVWIKDGRLHLRQKGHKDGDDGPISVAEVDSKRTDFQHGSFRAKFKVTVDKKAVGGAVAGFFFYYDDEDESDIEILTRDGDKTIRYTQHPAYDSATDTTIPDATFQIQLKKSWSKMQEHRFDWTPSKTTFFQDNSAIKEMTKNVPKHEGKIMINLWANNGTWSGLPSTKDVKMEMEYIMMYFNTSASQSGNDKDFNSACEAAGGPENETVCWINDKSGVYSEDSKVTLSASPAASISQNLVLVVGVGLAALLLVWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6A2</accession>
    <name>D5G6A2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00001674001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the lipin family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430008">
        <property type="protein sequence ID" value="CAZ80045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835888.1">
        <property type="nucleotide sequence ID" value="XM_002835842.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6A2"/>
    <dbReference type="FunCoup" id="D5G6A2">
        <property type="interactions" value="406"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G6A2"/>
    <dbReference type="GeneID" id="9187716"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001674001"/>
    <dbReference type="eggNOG" id="KOG2116">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002546_3_0_1"/>
    <dbReference type="InParanoid" id="D5G6A2"/>
    <dbReference type="OMA" id="QDYSMKL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008195">
        <property type="term" value="F:phosphatidate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009062">
        <property type="term" value="P:fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000063">
        <property type="entry name" value="Nuclear elongation and deformation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026058">
        <property type="entry name" value="LIPIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007651">
        <property type="entry name" value="Lipin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013209">
        <property type="entry name" value="LNS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031315">
        <property type="entry name" value="LNS2/PITP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057124">
        <property type="entry name" value="Ned1-like_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12181">
        <property type="entry name" value="LIPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12181:SF12">
        <property type="entry name" value="PHOSPHATIDATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04571">
        <property type="entry name" value="Lipin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08235">
        <property type="entry name" value="LNS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24565">
        <property type="entry name" value="Ned1_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00775">
        <property type="entry name" value="LNS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LNS2/PITP" evidence="4">
        <location>
            <begin position="407"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="95"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="303"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="601"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="650"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="763"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="95"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="190"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="324"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="608"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="651"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="686"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="767"/>
            <end position="777"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ80045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="783" mass="86579" checksum="AFFB784C557EFB9F" modified="2010-06-15" version="1">MQYVRSISGSVSKTWNSINPATLSGAIDVIVVEQENGDLACSPFHVRFGKFSLLRPYEKKVEFRVNGQKTDYSMKLGEGGEAFFVFETNQIVPPEMQTSPLVSPSASPRSMASVPEPSSLQEPDYLNIADTSGRKADSTGDVLPNPRRARSDFGNATPLSSSPDNPRPASGDWSGTAVNLERSASEEVLPTSTRRYTMSGQGGPVRTRSPPPIDHAAAVERALALSRKLSISNIPTHITDRGDVMLDMNGYKLDQEESLQAEMIARKILAEELEGDYDIGSLIGADRNGNLWIYSSEEAKEKAGREEGISQGIDAVSDAGYSSDEARSDSSSESAKKRIDRLSTPPTTPPLPGQEEGGSKSYAKTLRLTSEQLKSLDLKPGANAISFSVNKATCTAFMYLWKSDVPIVISDIDGTITKSDALGHVLTMIGRDWTHLGVAKLYTDIAANGYHLLYLTSRSVGQADTTRNYLNGIVQDKYKLPKGPVIMSPDRTFSALRREVYLRKPEVFKMACLRDILNLFGARHNPFYAGFGNRLTDALSYRSVNIPSTRIFTINSYAEVSLDLLTLTKYKSSYVNMRDLVDHFFPPVGLLSTDEQYTDFNYWRDPVPDPEEFSDSEAEEAGEEDEDEDGEMADSYYEEHGEMAEIYMENADGEDEYDEDEEEDGEEDDDEEEEDEEYEEEESETAESKIEQHPNGAQKEREELTAAAKAEEKTESKIPFVGGRMGGMKMDLDTNRLTQAKEFAERELSGIVDEVVGEIKALTTDSSKPEAENKGQEEIDVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6K6</accession>
    <name>D5G6K6_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">dolichol kinase</fullName>
            <ecNumber evidence="3">2.7.1.108</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00001815001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the polyprenol kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.108" evidence="3"/>
    <dbReference type="EMBL" id="FN430010">
        <property type="protein sequence ID" value="CAZ80149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835992.1">
        <property type="nucleotide sequence ID" value="XM_002835946.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6K6"/>
    <dbReference type="STRING" id="656061.D5G6K6"/>
    <dbReference type="GeneID" id="9187179"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001815001"/>
    <dbReference type="eggNOG" id="KOG2468">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007859_0_0_1"/>
    <dbReference type="InParanoid" id="D5G6K6"/>
    <dbReference type="OMA" id="HGTMVAM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004168">
        <property type="term" value="F:dolichol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043048">
        <property type="term" value="P:dolichyl monophosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032974">
        <property type="entry name" value="Polypren_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13205:SF15">
        <property type="entry name" value="DOLICHOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13205">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 15-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="36"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="70"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="300"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="373"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="464"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="505"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="547"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ80149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="615" mass="67130" checksum="083FAC93EDED621F" modified="2010-06-15" version="1">MRRGVEIGLMGVLVGIVLSSREGRIWKEVGIRKQEFAAWAGVLSTTLVYHPLLILLHGDPLVPPRSLDPSPLLYPIVFPIMVALSMTQENTMSQNPYVVSNLILSLSSLPPTLIPRWDLRWILSLLPLAIPAHFHRQLNSKSNSDALSFLPPLHFVLSSVLSYLLHPSLTPTELRMIATALINLLIYASSPQAIILKAVLWGGGLGILILCEDVIKWNVNLARVPSHKLRRAGNAIISIGRWKKLTSLRAKGQASDSEGELLRIRSKASPSARPKFVSQKSFYASLTHEQARKRKLAYSLWVYGSVACVVMVALRPYIGKYALDGVDPFLWALGYLLCGQSWYQNLVDTIAPGSGYCVTEGAASAANLRLLIIGYWLVVLAMGISLVTAILAHIQVDTRRKVFHGMVVIMFLIPGVLDPAFTYLGLSLTLAVFLLLDTVRAGQLPPFSGKIAVFLQPFVDGRDLKGPVIVSHVFLLLGCAIGWWFTLASASTEEGDAWDWSGRHIDLAFVSGVVCVGLGDAAASLIGRRFGRTKWGWRGGKSIEGSLAFTLAVMIGLSVGRWWVGTETQTWTPVVWAKIATVGLWGSMVEAVVTGVNDNIVVPVGAWIVVRSLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6M7</accession>
    <name>D5G6M7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00002133001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430012">
        <property type="protein sequence ID" value="CAZ80170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836013.1">
        <property type="nucleotide sequence ID" value="XM_002835967.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6M7"/>
    <dbReference type="STRING" id="656061.D5G6M7"/>
    <dbReference type="GeneID" id="9187196"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002133001"/>
    <dbReference type="eggNOG" id="ENOG502S7GA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095735_1_0_1"/>
    <dbReference type="InParanoid" id="D5G6M7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034444">
        <property type="entry name" value="Nuo17.8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42100">
        <property type="entry name" value="OXIDOREDUCTASE 178 KDA SUBUNIT, PUTATIVE (AFU_ORTHOLOGUE AFUA_8G04320)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42100:SF1">
        <property type="entry name" value="OXIDOREDUCTASE 178 KDA SUBUNIT, PUTATIVE (AFU_ORTHOLOGUE AFUA_8G04320)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="171" mass="19225" checksum="00965A32B8957860" modified="2010-06-15" version="1">MLVLRSSCRAILYSSRVVLGRRYDATLTGAQKGKAPPPNAPNGQMNHSTPPPEESFGKGFYLSIAAVVGTIALYKLEQSISGSDRSPFTRAIEYYSDVQDLWEKRNTRRTVLIERAAADRTLFLNSEPNKTVQVKFPEMLNNFSPRNNEVGWAQSVDLSTLREHYESQRGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6U2</accession>
    <name>D5G6U2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00002112001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RMD1/sif2 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430015">
        <property type="protein sequence ID" value="CAZ80233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836076.1">
        <property type="nucleotide sequence ID" value="XM_002836030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6U2"/>
    <dbReference type="FunCoup" id="D5G6U2">
        <property type="interactions" value="92"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G6U2"/>
    <dbReference type="GeneID" id="9182457"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002112001"/>
    <dbReference type="eggNOG" id="KOG2861">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011220_1_1_1"/>
    <dbReference type="InParanoid" id="D5G6U2"/>
    <dbReference type="OMA" id="HHTHPKL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003734">
        <property type="entry name" value="DUF155"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051624">
        <property type="entry name" value="RMD1/Sad1-interacting"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16255">
        <property type="entry name" value="REQUIRED FOR MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16255:SF15">
        <property type="entry name" value="SPORULATION PROTEIN RMD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02582">
        <property type="entry name" value="DUF155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="410"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="DUF155" evidence="4">
        <location>
            <begin position="211"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="11"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ80233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="436" mass="48840" checksum="4653A8543C772938" modified="2010-06-15" version="1">MATMSIPTPTPSTRAVVASSASASSATMSAATLTPPLLPARASNSPQLTPTNLPKGRRRSSSIHGQHPPYSQPHTAKIGPQRTSKVSQKLKILPSPADQDEESGRDVYSQVTRIKDTTARRDAERLGKAERAHLPRVTAYATANAYKMESMMKFLKSRQTSRGTAPKQFDECIYTPFSYTYEARQREQQQQGQRHGQSGDLIDPGVESSEVFLFQYGVVVIWGMSMRDEKRFLKDIAKFESEKLGEDDVQVENFNYYVTSSYQPRIYNDFITLKDGGNYMIKLSISHAIAQSVKISLFEDLVDNTIEDTKSIPQDVATTGKVNMSRRDIMKHIGELFILRININLQFSVLDSPELMWAEPQLDPVYQAARSYLEINQRVSLLNQRLDVIGDLLQMLKEQMSHSQGEHLEWIVIVLIAAEILVAVINVVVDLLAGSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6U9</accession>
    <name>D5G6U9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00002281001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430016">
        <property type="protein sequence ID" value="CAZ80242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836085.1">
        <property type="nucleotide sequence ID" value="XM_002836039.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6U9"/>
    <dbReference type="GeneID" id="9182467"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002281001"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_30_12_1"/>
    <dbReference type="InParanoid" id="D5G6U9"/>
    <dbReference type="OMA" id="TGSHMES"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17356">
        <property type="entry name" value="MFS_HXT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000026">
        <property type="entry name" value="MFS quinate transporter QutD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF35">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="7"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="80"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="104"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="145"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="175"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="266"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="301"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="331"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="376"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="410"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="442"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="11"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ80242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="525" mass="57618" checksum="4C5139D3E06C9DB1" modified="2010-06-15" version="1">MGYRVRNIYAICSFAAIGGGLFGFDISSMAGILGTNAYKNYFGNPLGARQGAITSAMPAGSLLGSLVSSYLGDYFGRKQAIQIGGIIWILGSTIQAAAQNVGMLVVGRIISGICVGITSTIVPVYQSELAPKEVRGRMVSLQQWAITWGILIQYFVQYGASFYGGGPTNKHQGPAAFRIPWSIQIIPAAILVGGMFFFPESPRWLAVKDRWEQALRVLADVHGNGDLNNPVVLAEYQEIEDIIRFEREEAISSFAELAKPRILRRVVLGMSIQMWSQLCGMNVMMYYIVYVMEGAGIGDPLLTASIQYIINVVMTVPAIIWMDKWGRRPTLLFGSFAMMSLLLISGSIQGVYGHPETDPSSAITWIMQNKGSQSKAVVACSYLFVATFAITWGPVSWTMPAEVFPNKVRAMAVSLSTASNWTWNCVLAFAVPPLLRTINWKMYMIFAAFNCAAFVHMFFTAPETKGVTLEEMDDVFDSGRPPWKTRSPVSRLDNLQKDIAAGNVKVDIGAATGRRTEIHQVPSKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6W6</accession>
    <name>D5G6W6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00002214001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430018">
        <property type="protein sequence ID" value="CAZ80259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842524.1">
        <property type="nucleotide sequence ID" value="XM_002842478.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6W6"/>
    <dbReference type="GeneID" id="9184074"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002214001"/>
    <dbReference type="HOGENOM" id="CLU_2869274_0_0_1"/>
    <dbReference type="InParanoid" id="D5G6W6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="64" mass="7241" checksum="D0D1E9F07BB2E468" modified="2010-06-15" version="1">MRISCLQRYLRTIRTLIIGPATGGLIIGWAFLAAIQRIVWSGSQGKVVYRRPPPDFDQLDLVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5G733</accession>
    <name>D5G733_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="12">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00002321001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SKN1/KRE6 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430021">
        <property type="protein sequence ID" value="CAZ80326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836135.1">
        <property type="nucleotide sequence ID" value="XM_002836089.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G733"/>
    <dbReference type="GeneID" id="9182517"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002321001"/>
    <dbReference type="eggNOG" id="ENOG502QR13">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010811_4_1_1"/>
    <dbReference type="InParanoid" id="D5G733"/>
    <dbReference type="OMA" id="LPACTCK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015926">
        <property type="term" value="F:glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006078">
        <property type="term" value="P:(1-&gt;6)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005629">
        <property type="entry name" value="Skn1/Kre6/Sbg1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31361">
        <property type="entry name" value="BETA-GLUCAN SYNTHESIS-ASSOCIATED PROTEIN KRE6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31361:SF1">
        <property type="entry name" value="BETA-GLUCAN SYNTHESIS-ASSOCIATED PROTEIN KRE6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03935">
        <property type="entry name" value="SKN1_KRE6_Sbg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="188"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="11">
        <location>
            <begin position="109"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="30"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="67"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ80326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="651" mass="71539" checksum="ED85741D5499192B" modified="2010-06-15" version="1">MSPRPLPPPITLNSSTLDLLNQNGNGRGGNPSSSVQSPSDSSPRINPSVRALTPQSPTVASPRAAFYQQSPVQSSDMLLPPSGQKRFQEYVDSLTSSRRSSWSADHGGSTYGPFVSPFDESRAPSRACSPDPMDVFNSQTISEKYNIMSSDGLLLFPVDKEDDDALHDPSEQDPDECEIWTRRGFVNVGGLAFIVLGLLCLFIGYPILTFVQDVIIEPESPCDAGELCIDIGPIDLLKNVRRSLIDPDTPESARTKKSASGKEYKLVVSAELSGRGKTRGDETEKKKMVYAATGDLEWYDPDAITTKDGYLEIEFAAFRNHDLDFRSGMLQSWNKMCFKGGIIEASLSLPGRGDVSGFWPGFWTMGNLGRPGYLATTEGMWPYTYHDECDAGITANQSSPDGISFLPGMKLPACTCAGEDHPSPGKSRSAPEIDAIEATVGAIDKEGKTLVGLASQSAQVAPFDLWYQPDYDHVAVYHPEFTAMNAYRGGFFQEAISGISILNNDWYDGKQYQTYGFEYEPGSEGMIEWNIGDLKTWRMNAASTGPNGNIGQRPIPNEPMSVIVNVGMGSSFAYIDFAEINKLLPAKMRVDWVRIWQDEDCEDCSVTCDPPGYPTTNYIEDHLEAFMNPNLTSWASTKHEWPKNTFANQCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7F8</accession>
    <name>D5G7F8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00002548001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430026">
        <property type="protein sequence ID" value="CAZ80451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836260.1">
        <property type="nucleotide sequence ID" value="XM_002836214.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7F8"/>
    <dbReference type="GeneID" id="9188475"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002548001"/>
    <dbReference type="HOGENOM" id="CLU_1442054_0_0_1"/>
    <dbReference type="InParanoid" id="D5G7F8"/>
    <dbReference type="OMA" id="NAAPKLM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072627" evidence="1">
        <location>
            <begin position="19"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="188" mass="18254" checksum="922CABDC83382CC3" modified="2010-06-15" version="1">MQFRFLIVALLTASVAHAADLIDINNEVTSRAGAATSVIGDVNSGLSRVTGAPSSIRSSIGSRVSSLVGDITNIASIASKAQSELSKFQTYSGYSSIASGFQSAITAGPAAASSYIANLPSNVDSSARSALASALSGAAAEVASSGTESSPSKTGSASATTSPNAAGKNTLVGSVLGAAGVLAVALAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7J3</accession>
    <name>D5G7J3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00002600001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430029">
        <property type="protein sequence ID" value="CAZ80486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836295.1">
        <property type="nucleotide sequence ID" value="XM_002836249.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7J3"/>
    <dbReference type="STRING" id="656061.D5G7J3"/>
    <dbReference type="GeneID" id="9186965"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002600001"/>
    <dbReference type="HOGENOM" id="CLU_378202_0_0_1"/>
    <dbReference type="InParanoid" id="D5G7J3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046496">
        <property type="entry name" value="DUF6589"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20231">
        <property type="entry name" value="DUF6589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6589" evidence="2">
        <location>
            <begin position="273"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="171"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ80486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="733" mass="82100" checksum="1BFE907104771B88" modified="2010-06-15" version="1">MGGSDLQVRGPGAPRINSRASKRKSVPTEPRPVGVDRPNPLEGKRSFVISQITYHPTPKDHLRADNFAPCATIPFIIRLPLSTPVLTKAMVKTSCTFRREKYLHTALAIPASSVPPIPRPLTSVNREQASGSAYPPASYFPYSFAQSALNATPGYDTSTSAPNGDYYFAQPNTSAAPGPTWPRNPAAAQGTEDDSRLLLTDQDGKIFWKNQSRLAVWRAMRRLWPKIGEILGESEVNTEPPYLATFSRVRGVPRVDESEIAQGEQPASRVCEDDLFPMPSMKRVSTKKIEEQLSILEMVTEYLGIEREHLKGSNPGNRLWLWAGNLEFVLALRKAKSIALPDSILHNIHPVLQLFDIREHFLLELLKQHYGRPNDKDPTSLCHHMQVMNRKFLIFGFSVTGQYAEGLLLHSLESHLIDSILTVTQVRESGPKTKREALDIITKVYDIYLEPEVVKNYQSALDPRTRDEIFENHMLLMQHSLVYLTLLDTISTASTTSLLHVLSWLSTAFQSLSFPISSSLGNELLDLVAGLTREWEPPLRRAVLDSMLIPKSPGAPLPAPAPHPASPKISGDYQEVDFAMKQLLRQTKAVFDLRDNPKHGFFREVATPNIRTISKVVRNAEESLGTAPRTANQWKSPAENVDPDKEIPKLVKELREARVGEWVPGRKAGFKVLHLFCNGEERVRSPGFLEEFVKRGDGGVIDGEGSGETDGRDKEWRRRNLFFAEDFGFDWGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7Z9</accession>
    <name>D5G7Z9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sulfate adenylyltransferase</fullName>
            <ecNumber evidence="9">2.7.7.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">ATP-sulfurylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Sulfate adenylate transferase</fullName>
            <shortName evidence="9">SAT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MET3</name>
        <name evidence="13" type="ORF">GSTUM_00002747001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the first intracellular reaction of sulfate assimilation, forming adenosine-5'-phosphosulfate (APS) from inorganic sulfate and ATP. Plays an important role in sulfate activation as a component of the biosynthesis pathway of sulfur-containing amino acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenosine 5'-phosphosulfate + ATP = 3'-phosphoadenylyl sulfate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24152"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58243"/>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>sulfate + ATP + H(+) = adenosine 5'-phosphosulfate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:18133"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58243"/>
            <dbReference type="EC" id="2.7.7.4"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="9">Allosterically inhibited by 3'-phosphoadenosine 5'-phosphosulfate (PAPS).</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8 9">Homohexamer. Dimer of trimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="9">The adenylyl-sulfate kinase (APS kinase) is non-functional. It is involved in allosteric regulation by PAPS. PAPS binding induces a large rotational rearrangement of domains lowering the substrate affinity of the enzyme.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">In the C-terminal section; belongs to the APS kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">In the N-terminal section; belongs to the sulfate adenylyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.4" evidence="9"/>
    <dbReference type="EMBL" id="FN430035">
        <property type="protein sequence ID" value="CAZ80642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836451.1">
        <property type="nucleotide sequence ID" value="XM_002836405.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7Z9"/>
    <dbReference type="FunCoup" id="D5G7Z9">
        <property type="interactions" value="539"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G7Z9"/>
    <dbReference type="GeneID" id="9184115"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002747001"/>
    <dbReference type="eggNOG" id="KOG0636">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022950_0_0_1"/>
    <dbReference type="InParanoid" id="D5G7Z9"/>
    <dbReference type="OMA" id="MEMRYAG"/>
    <dbReference type="UniPathway" id="UPA00097"/>
    <dbReference type="UniPathway" id="UPA00140">
        <property type="reaction ID" value="UER00204"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004020">
        <property type="term" value="F:adenylylsulfate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004781">
        <property type="term" value="F:sulfate adenylyltransferase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019344">
        <property type="term" value="P:cysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070814">
        <property type="term" value="P:hydrogen sulfide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010134">
        <property type="term" value="P:sulfate assimilation via adenylyl sulfate reduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019379">
        <property type="term" value="P:sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02027">
        <property type="entry name" value="APSK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00517">
        <property type="entry name" value="ATPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.400.10:FF:000003">
        <property type="entry name" value="Sulfate adenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000802">
        <property type="entry name" value="Sulfate adenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000052">
        <property type="entry name" value="Sulfate adenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.400.10">
        <property type="entry name" value="Sulfate adenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03106">
        <property type="entry name" value="Sulf_adenylyltr_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002891">
        <property type="entry name" value="APS"/>
    </dbReference>
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        <property type="entry name" value="sopT"/>
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    </dbReference>
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        <property type="entry name" value="SULFATE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF01583">
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    </dbReference>
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            <end position="380"/>
        </location>
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        </location>
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        </location>
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            <end position="566"/>
        </location>
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    </feature>
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        </location>
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="199"/>
            <end position="202"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
        </ligand>
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        <location>
            <begin position="293"/>
            <end position="296"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
        </ligand>
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    <feature type="binding site" evidence="9">
        <location>
            <position position="335"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
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        <location>
            <begin position="427"/>
            <end position="430"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
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        <location>
            <position position="508"/>
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        </ligand>
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        <location>
            <position position="205"/>
        </location>
    </feature>
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        <location>
            <position position="208"/>
        </location>
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            <position position="332"/>
        </location>
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    </evidence>
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        <source>
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    </evidence>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00062002"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF14306"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAZ80642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="566" mass="63240" checksum="ED95CF357529E3B2" modified="2010-06-15" version="1">MSDLPQPHGGVLKDLIARDLPRHAELLAESETLPALILTERQLCDLELILSGGFSPLEGFMNEKDYNGVVGNLRLVDGTLFSMPITLDVSKAQIEELGVEVGARITLRDFRDDRHLAIITVDDVYKPDKQKEAKEVLGSDEEHPAIRYLFNTAREYYVGGKIEAINRLNHYDYVGLRYTPAELRLHFEKLGWSKIVAFQTRNPMHRAHRELTVRAARARQANVLIHPVVGLTKPGDIDHFTRVRVYQALLPRYPNGMAVLGLLPLAMRMGGPREAIWHAIIRKNHGATHFIVGRDHAGPGSNSKGVEFYGPYDAQHAVARYKSELGIEVVPFQMMTYLPDTDEYAPTLNISGTELRRRLRTGTPIPEWFSYPEVVRVLRESHPPRHKQGFTVFLTGYTNSGKDAVARALQVTLNQQGGRSSTLLVGETVRSELSAELGFSREDRHKNIQRIAFVAAELTRAGAAVIAAPIAPFEDSRVQAKQTIEQFGSAYLIHVATPLAYCEKTDKRGVYARARSGEIKGFTGVDDPYEDPIKPDLVVDASKQTVRNIVHQIVLLLESEGLLDNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8B4</accession>
    <name>D5G8B4_TUBMM</name>
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        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00002962001</name>
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        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
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        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
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            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="CAZ80757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="eggNOG" id="ENOG502R9IN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023134_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8B4"/>
    <dbReference type="OMA" id="WNNDAHN"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039931">
        <property type="entry name" value="EEIG1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019448">
        <property type="entry name" value="NT-C2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21456:SF1">
        <property type="entry name" value="C2 NT-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21456">
        <property type="entry name" value="FAMILY WITH SEQUENCE SIMILARITY 102"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10358">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51840">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="61"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
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        </location>
    </feature>
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        <location>
            <begin position="280"/>
            <end position="384"/>
        </location>
    </feature>
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            <begin position="22"/>
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        </location>
    </feature>
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            <end position="314"/>
        </location>
    </feature>
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        </location>
    </feature>
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        <location>
            <begin position="342"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAZ80757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="402" mass="44177" checksum="69710F0CC4D3C05D" modified="2010-06-15" version="1">MQSLGMPSAYSILFPRATSTEQTTTFPMPQPTPSSTFSSTSSSSSSLRRGLLTRDLSFDGLIVPKNRRPRFDLTLEIHDLNNVPLVSGQAYVKWHIGGTARADFRGRTNKEGIKEHKVNWCYEQVCSNVRMTVDRSGMLQELPIVFEVIQEYAGARERIALGSVTLNLAEYTRVNKETRRYLMQDSKINSTLKVSISLEQTGGDPNFESPMLRGAQVFGGIAGIIGESQNQEDPGNATVATSQKRETSAIQDMYRCTLAASWQLQAGELHPEECIEDIFAGGDGWTPRSERGRAGKAKSVRYESDLSRSSKGSDSDSLSVVSAGAASGASSTNQAAMQRHTNTRDARRRKDGDTLSIMSNLTGPKVERKRSSRSSGSARSAYEVDELRAREDLVSWRMPSAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8G0</accession>
    <name>D5G8G0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00004810001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430042">
        <property type="protein sequence ID" value="CAZ80803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836612.1">
        <property type="nucleotide sequence ID" value="XM_002836566.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8G0"/>
    <dbReference type="GeneID" id="9186023"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004810001"/>
    <dbReference type="eggNOG" id="ENOG502QV47">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012743_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8G0"/>
    <dbReference type="OMA" id="WWSRYGL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039966">
        <property type="entry name" value="C553.12c"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40467">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40467:SF1">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="235"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="277"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="308"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="364"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="404"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="437"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="462"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="510"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="554"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ80803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="621" mass="71080" checksum="CD3BC15218D972BA" modified="2010-06-15" version="1">MPSFFAFQQGSSDSRSLPPSETESLRFGRFRAIPLHLQQQRRSVSGLFTAPPEYSTLTRPLLSGAGGGYGSNGGGYGAINISRNGNAVRNVGDDNDEEEDEVDERWWLDRVLISPRKKTVVWMMEIWYRRLGVLVVLPAGITIVWCGIPFPKYPLSVPPAILVDVPPSSAQVHMESHGKNLLRILWTAVHNLLDISVIRNLISSSLHPLASRGRNDGEVNQQVSGPGHGEAVVCINFWFFLLWYYAFYNFVGLLWITKLFNLYSVNWWPSRLGFPPAFTLFNLLPLFLSLPIYYLFPPPLLNNNLTWILLTFTTMSGPVLISFLVLLSERRSALRTHTMRNPFHTTFSSLRWYRRQRWGLTRSFTRFIWFCSALILSLIAFVLGEAYAEVYLRTLPHTSLETVIYVWSWIATIHLLDATTGWILGTKVGSYPLGWVFKLYFATTYQTYVRALYARLRSPSQFAYLQLLSSSIVIVYHPLSMTPLFHKAYTRLGLTDQTYREYQKYLGRGFFVRGLAENVTMVAWLGWVLALHYGWNREVYPYFSFDEFNFKLTFYASVMTWACEVIAGWCVRGIVRRGFGFEVMGEGVKDLVGFDGLLPAVLAVQVHVLQNMLVSIARLRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8Z4</accession>
    <name>D5G8Z4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00004908001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S28 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430053">
        <property type="protein sequence ID" value="CAZ80987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836796.1">
        <property type="nucleotide sequence ID" value="XM_002836750.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8Z4"/>
    <dbReference type="ESTHER" id="tubmm-d5g8z4">
        <property type="family name" value="Prolylcarboxypeptidase"/>
    </dbReference>
    <dbReference type="MEROPS" id="S28.004"/>
    <dbReference type="GeneID" id="9181270"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004908001"/>
    <dbReference type="eggNOG" id="KOG2182">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023630_2_0_1"/>
    <dbReference type="InParanoid" id="D5G8Z4"/>
    <dbReference type="OMA" id="YHVNDAY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070008">
        <property type="term" value="F:serine-type exopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008758">
        <property type="entry name" value="Peptidase_S28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11010:SF109">
        <property type="entry name" value="PEPTIDASE, FAMILY S28, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G03790)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11010">
        <property type="entry name" value="PROTEASE S28 PRO-X CARBOXYPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05577">
        <property type="entry name" value="Peptidase_S28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072248" evidence="7">
        <location>
            <begin position="19"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="495"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="597"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="506"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="608"/>
            <end position="630"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ80987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="657" mass="72278" checksum="1DD674112F3BB596" modified="2010-06-15" version="1">MFFPLFLMLALVATQAVASLLRPNSVRLSDYHLTEIINRDILKPALENDDLPTAPKRYVELPLNHGDPKSPKFKNRYWVDDTYYSPGGPIFFVDNGEADADGMEEYLRKGATGSLAKEFNGLLILWEHRFYGTSMPDMTNAMRFTSDNFGAYLKYHTIEQALEDVVVFAKQFTFNNKTVSPGEVPWVYLGGSYPGARSAWMRIRNPDIFHVSLASSAVVQLQKNMWQYYRVIEETLDKTGYANCSRDIRNITKWVDNAFDDQNGPAVDQFLEKITGPENSDLWNFFHFDDSSDPGEIWDNRRMNVKAAIGVVYRDFQYVGMNGILRTFCDGLQSNSGDSAGVGADFRNARGIFETHNTSAAIDIYTSVLAALWPKVLKPTDYPADGVKMDQVYNVNCARNQGLCKQIVTKWSWIYQTCIEFGAYQTANISRPTNLLPKFETIENELRDCKGWFGNAVAGGPNLDPINKKYGGWNMNPSNVFWTDGEIDPWRALTPNSIEDGAPKRPSTTNIPPSGSNAGGDTFFGFVMKGGSHCADLGQYVGQGLRNRTGAAPDTTGIKVPAGYVPEADTAHNLFKDALRTWLPAFKAHALSRESSFKVDTTGSSSSGDGGSGGEGSAGGGGNRPSSGGGDKSLAISLSASRCFYSAVVVVVVCAML</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>D5G999</accession>
    <name>D5G999_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00003211001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430056">
        <property type="protein sequence ID" value="CAZ81092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836901.1">
        <property type="nucleotide sequence ID" value="XM_002836855.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G999"/>
    <dbReference type="GeneID" id="9184378"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003211001"/>
    <dbReference type="HOGENOM" id="CLU_1062422_0_0_1"/>
    <dbReference type="InParanoid" id="D5G999"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="229"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="144"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="161"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ81092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="262" mass="30177" checksum="24745A187E2E3EA6" modified="2010-06-15" version="1">MPLSFTKFQVDVEPGMSARHSQITIERNRTPTPNPRVVICSPPPPPPPSYPPSVTQYTPYPETQVILAAPQEQDPEPRPAILNINIGQAASDIAEKMATENMWRNQVAVLEGSLEEERRARSKEASARYQREIEGIYEAREARRQLRSGGEHRKSRSTEISVEHREEAARQRISRQYLQRRSSSLSRLASSDGLLLAPEHSNPRHSDGRLSITHTHTRYHAHETRYHDCSYCNRRGHRSYECPERESEVIRVPVGRVRYWRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9E9</accession>
    <name>D5G9E9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">tRNA (guanine(10)-N(2))-methyltransferase</fullName>
            <ecNumber evidence="9">2.1.1.214</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00003275001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM11 methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.214" evidence="9"/>
    <dbReference type="EMBL" id="FN430060">
        <property type="protein sequence ID" value="CAZ81142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836951.1">
        <property type="nucleotide sequence ID" value="XM_002836905.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9E9"/>
    <dbReference type="FunCoup" id="D5G9E9">
        <property type="interactions" value="764"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G9E9"/>
    <dbReference type="GeneID" id="9182892"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003275001"/>
    <dbReference type="eggNOG" id="KOG2671">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029646_3_0_1"/>
    <dbReference type="InParanoid" id="D5G9E9"/>
    <dbReference type="OMA" id="AFNKWSR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043528">
        <property type="term" value="C:tRNA (m2G10) methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160102">
        <property type="term" value="F:tRNA (guanine(10)-N2)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000241">
        <property type="entry name" value="RlmKL-like_Mtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016691">
        <property type="entry name" value="TRMT11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059073">
        <property type="entry name" value="TRMT11_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13370">
        <property type="entry name" value="RNA METHYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13370:SF3">
        <property type="entry name" value="TRNA (GUANINE(10)-N2)-METHYLTRANSFERASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25904">
        <property type="entry name" value="Tmrp11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01170">
        <property type="entry name" value="UPF0020"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017259">
        <property type="entry name" value="tRNA_mtfrase_TRM11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51627">
        <property type="entry name" value="SAM_MT_TRM11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 10" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="3 10" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="tRNA (guanine(10)-N(2))-methyltransferase TRMT11 N-terminal" evidence="13">
        <location>
            <begin position="10"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal RNA large subunit methyltransferase K/L-like methyltransferase" evidence="12">
        <location>
            <begin position="184"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="403"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="411"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00066937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF25904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ81142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="444" mass="50046" checksum="5B0142AD8D896C0F" modified="2010-06-15" version="1">MSEEPSQLREYLIYFAQIHETFRKAELDSLSQLTGVGVQWKEYSDDSPFAIVHLPNNSSAKTLVERSILAKGVYELWGAGPNYDHLHATIKSQTPYLWLSCMSRSFKFEIECYNGKRKQSAQREIIESFAYLGLDGEIRMKDPDIIFTVLEEFPEKVATPTRIFFGRRVGSGSRALVERYDLKKRHYIGTTSMDAELALVTANMALVAPGKIAFDPFVGTGSFVVSCAHFGGLCLGSDIDGRQIRGKNGRSVLSNFQQYGLVGNYLDGFVSDLTNTPIRQTAFIDAILCDPPYGVREGLKVLGSRDPEKGKEPVIRGGVLRHLQDDYVPPKRPYSFLAMLDDIMQFAADHLVPGGRLCFWMPTANEDFSKLDIPSHPQLQLVSVCVQEFNKWSRRLITYSRIQGDAQAPPTREREERNGSKKATADELNSFRKRYFEGFRSSGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9M6</accession>
    <name>D5G9M6_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ubiquinone biosynthesis protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00003331001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Membrane-associated protein that warps the membrane surface to access and bind aromatic isoprenes with high specificity, including ubiquinone (CoQ) isoprene intermediates and presents them directly to Coq7, therefore facilitating the Coq7-mediated hydroxylase step. Participates in the biosynthesis of coenzyme Q, also named ubiquinone, an essential lipid-soluble electron transporter for aerobic cellular respiration.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the COQ9 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430063">
        <property type="protein sequence ID" value="CAZ81219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837028.1">
        <property type="nucleotide sequence ID" value="XM_002836982.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9M6"/>
    <dbReference type="FunCoup" id="D5G9M6">
        <property type="interactions" value="227"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G9M6"/>
    <dbReference type="GeneID" id="9185355"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003331001"/>
    <dbReference type="eggNOG" id="KOG2969">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057411_1_1_1"/>
    <dbReference type="InParanoid" id="D5G9M6"/>
    <dbReference type="OMA" id="CAGFGWN"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.10:FF:000004">
        <property type="entry name" value="Ubiquinone biosynthesis protein COQ9, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.10">
        <property type="entry name" value="Tetracycline Repressor, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013718">
        <property type="entry name" value="COQ9_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012762">
        <property type="entry name" value="Ubiq_biosynth_COQ9"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02396">
        <property type="entry name" value="diverge_rpsU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21427">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS PROTEIN COQ9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21427:SF19">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS PROTEIN COQ9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08511">
        <property type="entry name" value="COQ9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="7 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 8" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="domain" description="COQ9 C-terminal" evidence="9">
        <location>
            <begin position="173"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ81219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="273" mass="30435" checksum="C09431D0E2D1713A" modified="2010-06-15" version="1">MTVRTNLSHPSIRWMLSKPITSASAASCWSSLTAPRINLPRRYHSYQHPAPGPSSTTAKSAILSASLPHVPSHGFSDTALAFGSRDAGYLDISVNMLPGGVFDLVRYHLVTERLRLGDKIDFRKPENELPEGLRNLDITTKIQILCMERLKANAHIIHRWQEALAVMSLTENIPASMSELAKLSDEIWFLAGDESHDTTWYTKRATLAVVYASTELFMTHDNSKDFEATQEFLGRRLNDLKTAGSAVSNVASYLDFTVHAFANVLRSKNVPFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9N0</accession>
    <name>D5G9N0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00004981001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430064">
        <property type="protein sequence ID" value="CAZ81223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837032.1">
        <property type="nucleotide sequence ID" value="XM_002836986.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9N0"/>
    <dbReference type="GeneID" id="9185360"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004981001"/>
    <dbReference type="eggNOG" id="ENOG502T27G">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1074391_0_0_1"/>
    <dbReference type="InParanoid" id="D5G9N0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="238"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="38"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="144"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="259" mass="28118" checksum="16AE2887D0AB0308" modified="2010-06-15" version="1">MTTRTTTPLSYTSSTTYHTTTPEPQGLSPPPTRRQRSHSTNSHNSWWSAEEPSPVSLAAWGVSIATTAMGAGASSPRKQKTSSTMGTVTPGRRRGQASCSRTITTPGSSSSSASTIRPHYQLHYNPHHHHRHHHHHHHPTSSRSPVTTAAPSFSSSSSTSSYTSTHPTLSPTLPYNTPIDWKGNAARRAEYAALDRRRQGVTGLFRRAAACCFGRARSEFWEEKCGDDGGSVRRYRLALPAEEEEEDPSSKGAGEGGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9N3</accession>
    <name>D5G9N3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00004987001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the chromate ion transporter (CHR) (TC 2.A.51) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430064">
        <property type="protein sequence ID" value="CAZ81226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837035.1">
        <property type="nucleotide sequence ID" value="XM_002836989.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9N3"/>
    <dbReference type="GeneID" id="9185363"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004987001"/>
    <dbReference type="eggNOG" id="ENOG502QRJG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018106_0_2_1"/>
    <dbReference type="InParanoid" id="D5G9N3"/>
    <dbReference type="OMA" id="VWGMART"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015109">
        <property type="term" value="F:chromate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003370">
        <property type="entry name" value="Chromate_transpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33567">
        <property type="entry name" value="CHROMATE ION TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33567:SF3">
        <property type="entry name" value="CHROMATE ION TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02417">
        <property type="entry name" value="Chromate_transp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="92"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="139"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="191"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="303"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="333"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="370"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="403"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="440"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="497"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="222"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="260"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ81226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="518" mass="55607" checksum="778899DD1E7A8908" modified="2010-06-15" version="1">MPLSAIFRSLQSNQPDAYQAMKGGLGLRLWEIVANYWDLGFTSFGGPPVHFQILRKRFVDAENGRIPWIDDQTYQELFAICQALPGPASTKFAFCIALIHAGFIPAFLAFLIWSLPGAIGMFALALGVERIGEVLPDPVYGLLSGLNASTVGIIALAAVQLAEKAITDPLTRMLVILGACAGLPYNALWYFPLLMAIGGIITIFWDLWGRAKFGRMRARFSRKNRNEQREAEESIASGAVPLENRNPARDGGLQRRAAGRAEASGATASPAPGDSENEITAEAEATPQPQGIDTVSHAIPVKVGILIIIGFFASFIALMSVRGLDNTPLEFDLFANMYLAGTIIFGGGPVVIPLLREYVVEPGWVSSRDFLLGLAIIQAFPGPNFNFAVYLGALALNRGSSSVLGAFLGFLGIFFPGITLSIGVQSIWRVLRTKPAVVSLLRGVNATAVGLVFTAVYRLWQIGYLQGGQDGSGSLGLEPWWVVVAAVTYSSVQWWNVPPPVAILGGAVGGIAWWGVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5GA05</accession>
    <name>D5GA05_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Phosphatidyl-N-methylethanolamine N-methyltransferase</fullName>
            <ecNumber evidence="18">2.1.1.71</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Phospholipid methyltransferase</fullName>
            <shortName evidence="18">PLMT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">GSTUM_00003480001</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17 18">Catalyzes the second two steps of the methylation pathway of phosphatidylcholine biosynthesis, the SAM-dependent methylation of phosphatidylmonomethylethanolamine (PMME) to phosphatidyldimethylethanolamine (PDME) and of PDME to phosphatidylcholine (PC).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-N,N-dimethylethanolamine + S-adenosyl-L-methionine = a 1,2-diacyl-sn-glycero-3-phosphocholine + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32739"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64572"/>
            <dbReference type="EC" id="2.1.1.71"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 18">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-N-methylethanolamine + S-adenosyl-L-methionine = a 1,2-diacyl-sn-glycero-3-phospho-N,N-dimethylethanolamine + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32735"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64572"/>
            <dbReference type="ChEBI" id="CHEBI:64573"/>
            <dbReference type="EC" id="2.1.1.71"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 18">Phospholipid metabolism; phosphatidylcholine biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="18">Endoplasmic reticulum membrane</location>
            <topology evidence="18">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Mitochondrion membrane</location>
            <topology evidence="18">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the class VI-like SAM-binding methyltransferase superfamily. PEMT/PEM2 methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.71" evidence="18"/>
    <dbReference type="EMBL" id="FN430068">
        <property type="protein sequence ID" value="CAZ81348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837157.1">
        <property type="nucleotide sequence ID" value="XM_002837111.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GA05"/>
    <dbReference type="FunCoup" id="D5GA05">
        <property type="interactions" value="48"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GA05"/>
    <dbReference type="GeneID" id="9187046"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003480001"/>
    <dbReference type="eggNOG" id="KOG4142">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086119_0_0_1"/>
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    <dbReference type="UniPathway" id="UPA00753"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0000773">
        <property type="term" value="F:phosphatidyl-N-methylethanolamine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006656">
        <property type="term" value="P:phosphatidylcholine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1630:FF:000005">
        <property type="entry name" value="Phosphatidylethanolamine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03216">
        <property type="entry name" value="PLMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024960">
        <property type="entry name" value="PEMT/MFAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007318">
        <property type="entry name" value="Phopholipid_MeTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15458">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15458:SF5">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04191">
        <property type="entry name" value="PEMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005444">
        <property type="entry name" value="PEMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51599">
        <property type="entry name" value="SAM_PEMT_PEM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 18" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3 18" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10 18" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 18 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 18" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11 18" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13 18" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="14 18" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 18" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 18" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 18 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 18 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="topological domain" description="Lumenal" evidence="18">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="intramembrane region" description="Helical" evidence="18">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="topological domain" description="Lumenal" evidence="18">
        <location>
            <begin position="41"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="18">
        <location>
            <begin position="75"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="topological domain" description="Lumenal" evidence="18">
        <location>
            <begin position="123"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="156"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="18">
        <location>
            <begin position="187"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="106"/>
            <end position="108"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="188"/>
            <end position="189"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00051252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00052459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00056845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CAZ81348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="208" mass="23325" checksum="DC112EE39DF43213" modified="2010-06-15" version="1">MPTKLFANSTVMDLVDFDQKSLLVAAGSIAFNPIFWNVVARQEYHNKVLTKLFGGNSRYGCYFLAAVIFGIGIFRDFLYEQALRQQPSLDVLQTDAVKLAALALFFCGNVLVLTSMWALGFTGTYLGDYFGILMDHRVTSFPFNVTDAPMYYGSTMAFLGSALWFAKPAGILLTIEVFIVYKMALMFEDPFTGAIYAKRERERAKKST</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAA2</accession>
    <name>D5GAA2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Halotolerance protein 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00005200001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
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            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="FN430075">
        <property type="protein sequence ID" value="CAZ81456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837265.1">
        <property type="nucleotide sequence ID" value="XM_002837219.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAA2"/>
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        <property type="interactions" value="119"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GAA2"/>
    <dbReference type="GeneID" id="9185738"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005200001"/>
    <dbReference type="eggNOG" id="KOG0590">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_127_1_1"/>
    <dbReference type="InParanoid" id="D5GAA2"/>
    <dbReference type="OMA" id="QDSKCEQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045807">
        <property type="term" value="P:positive regulation of endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903329">
        <property type="term" value="P:regulation of iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd13994">
        <property type="entry name" value="STKc_HAL4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000183">
        <property type="entry name" value="Serine/threonine-protein kinase hal4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343:SF558">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343">
        <property type="entry name" value="SERINE/THREONINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="237"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="26"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="47"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="90"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="271"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00078109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ81456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="522" mass="57947" checksum="EDE6FF18E12CA950" modified="2010-06-15" version="1">MIEPPLQKNNASSSLNEGRSSPTMLTPAVSSSAPSTTASPVLLPVPGQSTSLSSLPGSLMCPGTATPPNIPEKKSISSRLHRMFSQSGGKAKEESKGTPRERHVGEGSASDTDSSALSGREKDITTSPDQKSSSTEKRPAANATTDNKNGLPAVLQRFQMLPDGTHEHHLRSAKRQEKLSDMLRDLIGGKKKDDHGEHDRLSLMSSWVDQLKIDRDKLASDRKGGPNSTATLVEKYGKCQEVIGRGAFGIVRISHKPDPKNGHSEQLYAVKEFRRRPQESPRKYHKRLTSEFCISSSLRHQNVIHTLDLLQDAKGDYCEVMEFCAGGDLYTLVLTAGKLDVAEADCYFKQLMRGVEYMHQMGVAHRDLKPENLLLTTNGALKITDFGNGECFRMAWEKEAHMTCGLCGSAPYIAPEEYTDKEFDPRAVDVWATGVIYMAMRTGRHLWRVAKKDEDEFFEKYLQGRKDEGGYAPIENLHRARCRNVIYSILDPNPTRRITASQVLKSEWGREITVCKEGEEGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAD9</accession>
    <name>D5GAD9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00005116001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430075">
        <property type="protein sequence ID" value="CAZ81396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837205.1">
        <property type="nucleotide sequence ID" value="XM_002837159.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAD9"/>
    <dbReference type="FunCoup" id="D5GAD9">
        <property type="interactions" value="197"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GAD9"/>
    <dbReference type="GeneID" id="9187094"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005116001"/>
    <dbReference type="eggNOG" id="KOG0016">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_6_2_1"/>
    <dbReference type="InParanoid" id="D5GAD9"/>
    <dbReference type="OMA" id="FQAIMDF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004165">
        <property type="term" value="F:delta(3)-delta(2)-enoyl-CoA isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000048">
        <property type="entry name" value="3,2-trans-enoyl-CoA isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051053">
        <property type="entry name" value="ECH/Chromodomain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43684">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43684:SF1">
        <property type="entry name" value="ENOYL-COA DELTA ISOMERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ81396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="264" mass="28973" checksum="BE502AFB867D70F5" modified="2010-06-15" version="1">MSEPILLEVKKNVALITLNQPKKLNALTGDLYFGLAQVMHKVAAMEEVTITVLTGTGRYFSAGVDLSSVLGEGRDDNERKHYLRRLAANNMEVTKAFFEHPKILVVALNGPAVGLSAALVAMADFIYATEKTFILTPFTSLGLVAEGGSSYMFLRRMGVAKAHEALILSKRIPAADLIACGFVNKIFPEEGFRDRVLEFVNDAMGDHLVHESMLGVKKLMRDGMKKDLESASVREAFAGVERFLSGVPQNEFARITSGQKKHKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAK1</accession>
    <name>D5GAK1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cytoplasmic tRNA 2-thiolation protein 1</fullName>
            <ecNumber evidence="6">2.7.7.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Cytoplasmic tRNA adenylyltransferase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">NCS6</name>
        <name evidence="6" type="synonym">CTU1</name>
        <name evidence="10" type="ORF">GSTUM_00003620001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position. Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation. May also be involved in protein urmylation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.-" evidence="6"/>
    <dbReference type="EMBL" id="FN430078">
        <property type="protein sequence ID" value="CAZ81544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837353.1">
        <property type="nucleotide sequence ID" value="XM_002837307.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAK1"/>
    <dbReference type="FunCoup" id="D5GAK1">
        <property type="interactions" value="432"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GAK1"/>
    <dbReference type="GeneID" id="9187484"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003620001"/>
    <dbReference type="eggNOG" id="KOG2840">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026481_1_0_1"/>
    <dbReference type="InParanoid" id="D5GAK1"/>
    <dbReference type="OMA" id="KPVRGIC"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002144">
        <property type="term" value="C:cytosolic tRNA wobble base thiouridylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032447">
        <property type="term" value="P:protein urmylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002143">
        <property type="term" value="P:tRNA wobble position uridine thiolation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01713">
        <property type="entry name" value="CTU1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000054">
        <property type="entry name" value="Cytoplasmic tRNA 2-thiolation protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03053">
        <property type="entry name" value="CTU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056369">
        <property type="entry name" value="CTU1-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032442">
        <property type="entry name" value="CTU1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000541">
        <property type="entry name" value="Ncs6/Tuc1/Ctu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011063">
        <property type="entry name" value="TilS/TtcA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035107">
        <property type="entry name" value="tRNA_thiolation_TtcA_Ctu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020554">
        <property type="entry name" value="UPF0021_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00269">
        <property type="entry name" value="TIGR00269 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11807">
        <property type="entry name" value="ATPASES OF THE PP SUPERFAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11807:SF12">
        <property type="entry name" value="CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01171">
        <property type="entry name" value="ATP_bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16503">
        <property type="entry name" value="zn-ribbon_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004976">
        <property type="entry name" value="ATPase_YdaO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01263">
        <property type="entry name" value="UPF0021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 6" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="2 6" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="tRNA(Ile)-lysidine/2-thiocytidine synthase N-terminal" evidence="8">
        <location>
            <begin position="61"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="Cytoplasmic tRNA 2-thiolation protein 1 C-terminal" evidence="9">
        <location>
            <begin position="290"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="64"/>
            <end position="66"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR004976-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ81544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="352" mass="38824" checksum="42F7FF3CA345CC7C" modified="2010-06-15" version="1">MPPALPIPASKLCESCLSARALISRPKTHQKLCKPCFLSTFESEIHATIVSTNLFTKGSRIAIGASGGKDSTVLASVLKTLNERYDYGVEFVLLSIDEGIKGYRDDSLETVKRNAIQYGMELVIVSYEELYGWSMDSVVESIGKKGNCTYCGVFRRQALDRGAERLGIHHVVTGHNADDIAETVLMNLLRGDLPRLHRCTSIITSSTSSPIARSKPLKYAYEKEIVLYALHKKLDYFSTECIYSPEAFRGSARTLIKALEKVRPSAILDIVRSGEAFSRMIPSQAQAMSNCARCGYLTSQKLCKACTLLEGLNKNRPKTIVEVEAGIEDEEVSSSLWRGIEAIKLVEEGIAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAT7</accession>
    <name>D5GAT7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00005301001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430086">
        <property type="protein sequence ID" value="CAZ81630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837439.1">
        <property type="nucleotide sequence ID" value="XM_002837393.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAT7"/>
    <dbReference type="STRING" id="656061.D5GAT7"/>
    <dbReference type="GeneID" id="9187797"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005301001"/>
    <dbReference type="eggNOG" id="KOG1181">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_311047_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAT7"/>
    <dbReference type="OMA" id="YHHSPHH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="181"/>
            <end position="216"/>
        </location>
    </feature>
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        <location>
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            <end position="240"/>
        </location>
    </feature>
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        </location>
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        </location>
    </feature>
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            <end position="320"/>
        </location>
    </feature>
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        </location>
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            <begin position="581"/>
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        </location>
    </feature>
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        </location>
    </feature>
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            <begin position="689"/>
            <end position="713"/>
        </location>
    </feature>
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        <location>
            <begin position="714"/>
            <end position="745"/>
        </location>
    </feature>
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        <location>
            <begin position="759"/>
            <end position="774"/>
        </location>
    </feature>
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        <location>
            <begin position="795"/>
            <end position="806"/>
        </location>
    </feature>
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        <location>
            <begin position="836"/>
            <end position="846"/>
        </location>
    </feature>
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        <location>
            <begin position="847"/>
            <end position="864"/>
        </location>
    </feature>
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        <location>
            <begin position="887"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="898"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="911"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="934"/>
            <end position="945"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="945" mass="107953" checksum="47DE0DED599F8AC4" modified="2010-06-15" version="1">MLVPQDPPQTLPPSQLPLPSQAMLLQSAQPVRPVINLEDDVAPIKQQPAYSPPPVPVPPPQPQFQDIRQQPLRQVPVVHKHMPPLQRQQQREQQQREQQQREQQQREQQQREQQQREQLQREQQQREQQQREQQQREQQQREQQQREQQQREQQQREQQQREQQQREQQQREQREQEHQQPQHLQQQHPHQQHPQPHLQQQHPQQHPQHQHPQQHLQQEHPRPLLQPHHPQQPQPPPAPVVVPSHSHPRVPPSSQQPPQQVQAQPPLVQAPIPVPVHRSFIQRTEETHSERPERRPERQLESSHDRTHSAQLERQVSGQVGRQVEAPVERPMDSRHSERQIEGPVKHLERPHGGGPQVGSREFAKQFERAYGGARDGTLERQRDVPLDRPRDVRMPERQAVGGQPSGGPLIERPHRVRQERMQERSEKSLQDRSLERSHEGLRTPHERRMGREAPSVPSLQGPSPSPHTPVSSTSAPQGKIISTQITIPKQQTHAQQIHHPSQQQQQQQLQQQQGRGSYNQQHQTNQPLHHRNLSSPSAIPMFDAQYPQQQQPFGQQPRAPPVEKPQPSRAPEPLAPPSSRPMSAPSSGQPMNTPYNPPPPSHLGSTPQIPRQPSLPPQAAAVHVPPPVQSQSTAAPDSSKSKPTSINSLLNPAKEEIPSRPQSRSTPHHIPPQTRPQTPQQQPPPQHTPLQHASSQLAAAQHVTQHPPQHRSQPPHHSHHGSLQHLQHAPHSHHPHPMHHHHQHSQSSSTHAPIPGESSRRDVTYDLQHESHQQRPSMAKSPLSRGGPPPYDSVQLSREPSSYSTPPREPYLQPRESLYTPALRETPYSTPPPHSQSQQGLSGSHTPTSHSTLQPSNYAQGQHSHYPPRPQTCSPYRPGGPQSGPYVPPSSIPPSQPSHHSQQYPHYGQQPPPQPQPGNRHTPPPRGQYTQRRYYDGLDRGRPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAZ6</accession>
    <name>D5GAZ6_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">RuvB-like helicase</fullName>
            <ecNumber evidence="15">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">GSTUM_00005374001</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">DNA helicase participates in several chromatin remodeling complexes, including the SWR1 and the INO80 complexes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the RuvB family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="15"/>
    <dbReference type="EMBL" id="FN430086">
        <property type="protein sequence ID" value="CAZ81689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837498.1">
        <property type="nucleotide sequence ID" value="XM_002837452.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAZ6"/>
    <dbReference type="FunCoup" id="D5GAZ6">
        <property type="interactions" value="1364"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GAZ6"/>
    <dbReference type="GeneID" id="9187857"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005374001"/>
    <dbReference type="eggNOG" id="KOG2680">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028311_4_0_1"/>
    <dbReference type="InParanoid" id="D5GAZ6"/>
    <dbReference type="OMA" id="IINTEPY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097255">
        <property type="term" value="C:R2TP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000812">
        <property type="term" value="C:Swr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000492">
        <property type="term" value="P:box C/D snoRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002221">
        <property type="entry name" value="RuvB-like 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000010">
        <property type="entry name" value="RuvB-like helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.360:FF:000002">
        <property type="entry name" value="RuvB-like helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.360">
        <property type="entry name" value="RuvB-like helicase, domain II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027238">
        <property type="entry name" value="RuvB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041048">
        <property type="entry name" value="RuvB-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042487">
        <property type="entry name" value="RuvBL1/2_DNA/RNA_bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010339">
        <property type="entry name" value="TIP49_P-loop"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11093">
        <property type="entry name" value="RUVB-RELATED REPTIN AND PONTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06068">
        <property type="entry name" value="TIP49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17856">
        <property type="entry name" value="TIP49_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0010">Activator</keyword>
    <keyword evidence="7 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 15" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4 15" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12 15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6 15" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 15" id="KW-0804">Transcription</keyword>
    <keyword evidence="9 15" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="16">
        <location>
            <begin position="69"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAZ81689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="474" mass="51829" checksum="0B3DE29CAE41F2F7" modified="2010-06-15" version="1">MANLGITTVGEAKEIRGMNLIAAHSHIRGLGIDAETLEPRAASQGLVGQARARKAAAIILKMVQEGKIAGRAVLMAGPPSTGKTAIAMAMAQSLGPDVPFTMLSSSEIFSLDMSKTEALTQAFRQSIGVRIKEESEVIEGEVVEIQIDRSVTGGHKQGKLTMKTTDMETMYDLGTKMIDGLTKEKVMAGDIISIDKSSGKITKLGRSFTRSRDYDAMGPDTKFVQCPEGEIQTRKESVHTVSLHEIDVINSRTQGFLALFSGDTGEIRSEVRDQINIKVGEWKEEGKAEIVPGVLFIDEVHMLDIECFSYINRALESELSPIVIMASNRGNTRIRGTNYRSPHGLPLDFLDRVVIMPTYPYGPEEIKEILAIRAQEEEITLSPDALALLTKIGQETGLRYSSHLITTASLIAAKRKSSLVEVGDVQRSYKLFFDQGRSSQYLQEYEKKFISEEGNVTFAGGDTTAGGGDRMEIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAZ8</accession>
    <name>D5GAZ8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AP complex subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00005376001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Adaptins are components of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the adaptor complexes large subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430086">
        <property type="protein sequence ID" value="CAZ81691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837500.1">
        <property type="nucleotide sequence ID" value="XM_002837454.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAZ8"/>
    <dbReference type="FunCoup" id="D5GAZ8">
        <property type="interactions" value="812"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GAZ8"/>
    <dbReference type="GeneID" id="9187859"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005376001"/>
    <dbReference type="eggNOG" id="KOG1061">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006320_4_0_1"/>
    <dbReference type="InParanoid" id="D5GAZ8"/>
    <dbReference type="OMA" id="NPPEVQW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030117">
        <property type="term" value="C:membrane coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000058">
        <property type="entry name" value="AP complex subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026739">
        <property type="entry name" value="AP_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016342">
        <property type="entry name" value="AP_complex_bsu_1_2_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11134">
        <property type="entry name" value="ADAPTOR COMPLEX SUBUNIT BETA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002291">
        <property type="entry name" value="AP_complex_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin/coatomer adaptor adaptin-like N-terminal" evidence="7">
        <location>
            <begin position="16"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR002291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ81691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="725" mass="79739" checksum="FA77B008F65201BC" modified="2010-06-15" version="1">MAANRIRSMFPVAPRKGETFELRTGLNSQFAHERKDAIRNTIAAMTLGKDVSALFPDIIKNMATGDLDQKKLVYLYLMNYAKTHPDLCILAVNTFVQDSEDPNPLIRALAIRTMGCIRVDKIVDYMEEPLRKTLKDESPYVRKTAAICVAKLFDLNPSLCLENGFLTTLQEMIGDSNPMVVANSVTALAEINEAAPETEALAVTPAILKKLLMALNECTEWGRITILTTLAEYKAADVKEAEHICERVVPQFQHVNPSVVLAAVKVVFLHMKVISAELCRQYLRKMAPPLVTLVSSQPEVQYVALRNINLLLQKQPDILTKEMRVFFCKYNDPPYVKLEKLEIMIRIANEKNVDQLLSELKEYALEVDMDFVRRAVRAIGQCAIKIDSATGKCVNVLLELINTKVNYVVQEAIVVIKDIFRKYPGYEGIIPKLCESLDELDEPNARGSLIWVIGEYAEKINNADELLQTFMEGFKDEYTQVQLQLLTAGVKLFLKKPQSQAVVQQILQSATSECDNPDIRDRAYVYWRLLSKDPEVAKSIVLSDKPPITSTIQSLPVGLLEELLGEISTLASVYHKPAATFVGQGSFGADAVQKRAIEEQLQNARENPIQASAAAKGQNMENLLDIDFDGAAPASASTQTPQPGGLESLVIDSPARVASPTSPTSGTGNNLDDLLSLFGTESVAPANGSTNSGVLAGLEGLGLGSQAASQPQLQKKANEDILGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5GB29</accession>
    <name>D5GB29_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Dynactin subunit 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00005419001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the dynactin subunits 5/6 family. Dynactin subunit 5 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430086">
        <property type="protein sequence ID" value="CAZ81722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837531.1">
        <property type="nucleotide sequence ID" value="XM_002837485.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GB29"/>
    <dbReference type="STRING" id="656061.D5GB29"/>
    <dbReference type="GeneID" id="9187963"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005419001"/>
    <dbReference type="eggNOG" id="KOG3121">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088622_0_0_1"/>
    <dbReference type="InParanoid" id="D5GB29"/>
    <dbReference type="OMA" id="SQIHGTQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005869">
        <property type="term" value="C:dynactin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03359">
        <property type="entry name" value="LbH_Dynactin_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047125">
        <property type="entry name" value="DCTN5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46126">
        <property type="entry name" value="DYNACTIN SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46126:SF1">
        <property type="entry name" value="DYNACTIN SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21711">
        <property type="entry name" value="DCTN5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ81722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="231" mass="25072" checksum="834C780758EE7846" modified="2010-06-15" version="1">MPPKQTKGEYIETDTGNKVSRKSVILGSQNIILGGKTIIQADCTIRGDLRRTVAPSQQSGNVAIAVGRYCFLSRSSVLRPPGRTHQKPVPTYVSPRPPVVQGADQPLLCRTFSYCPMKIGDHVFVGEGSVVEAALIGSYVHIAKDCVIGKFAIIKDCVRIEEGTVIAPNTVIPSFSLVSGRPGVVTDELPETAQEGLECRLPNYLIYNIIYMIAGVQTLTIGVVKNIYRMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>D5GB98</accession>
    <name>D5GB98_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00000532001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430089">
        <property type="protein sequence ID" value="CAZ81791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837600.1">
        <property type="nucleotide sequence ID" value="XM_002837554.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GB98"/>
    <dbReference type="STRING" id="656061.D5GB98"/>
    <dbReference type="GeneID" id="9181502"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000532001"/>
    <dbReference type="eggNOG" id="ENOG502SCP9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068080_1_0_1"/>
    <dbReference type="InParanoid" id="D5GB98"/>
    <dbReference type="OMA" id="MSMFDAG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin type-2" evidence="1">
        <location>
            <begin position="55"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="251" mass="28330" checksum="8F0F8A4DE23FE538" modified="2010-06-15" version="1">MAPCVPKSFTAGQNPLVVFEGALEINNITPPKGRSFLFRVTFHVNHPRLKAIAATGKKPPLHFHPYQAEFFQVISGKVTIEVEGAERVLSAESGEVCIEPGPYHRLWPTPGSEENLIFDLSASDAPGVSLIVDQCFFENWYGYQNDVLMHEGKIDIIQVMNMFDAGDSYLALFPRWFPFGQTLAQFLGIAIGRWFGSLLGYKPFHPEWTTDWPGACDKMEKSIFYRRFADRRAQDVARKRFDNSSSSSKSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBM0</accession>
    <name>D5GBM0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00005702001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ82026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837835.1">
        <property type="nucleotide sequence ID" value="XM_002837789.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBM0"/>
    <dbReference type="GeneID" id="9185461"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005702001"/>
    <dbReference type="HOGENOM" id="CLU_1636646_0_0_1"/>
    <dbReference type="InParanoid" id="D5GBM0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ82026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="162" mass="18003" checksum="3A8E324058D19E11" modified="2010-06-15" version="1">MLINNPFPITEFAHTYGPSLLVAGITSATMVRERKRGTKELIQEIRRVGEKLGPDSCIESGTCHIEEKPKSVSDDAKDTKSMIPINGHHVASVLGENEKLMECLHSVLKDSKLFNDLKRIDGRQLMNALGENIKPMRECPHIPVQHIRSGGESKDYSSVKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBN3</accession>
    <name>D5GBN3_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">rRNA methyltransferase 2, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00005489001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ81883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837692.1">
        <property type="nucleotide sequence ID" value="XM_002837646.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBN3"/>
    <dbReference type="FunCoup" id="D5GBN3">
        <property type="interactions" value="13"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GBN3"/>
    <dbReference type="GeneID" id="9184457"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005489001"/>
    <dbReference type="eggNOG" id="KOG4589">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009422_2_0_1"/>
    <dbReference type="InParanoid" id="D5GBN3"/>
    <dbReference type="OMA" id="WSQVAVN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008650">
        <property type="term" value="F:rRNA (uridine-2'-O-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01547">
        <property type="entry name" value="RNA_methyltr_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050082">
        <property type="entry name" value="RNA_methyltr_RlmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015507">
        <property type="entry name" value="rRNA-MeTfrase_E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920">
        <property type="entry name" value="RIBOSOMAL RNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920:SF18">
        <property type="entry name" value="RRNA METHYLTRANSFERASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribosomal RNA methyltransferase FtsJ" evidence="7">
        <location>
            <begin position="113"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00041184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ81883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="354" mass="39794" checksum="410676C87BDF477D" modified="2010-06-15" version="1">MPQSKFPEPRIRVTTPECGSAHKFEPPIKLWTTPPPHHPTIYVVRKTGNMAATTIPRRIASPPLFFSPPTAYTHLLKSSCASSIRYDSSSSNQWKRRQIRDVFATAAKRDDMKSRAGYKLIELDYKHGIFGPGQTVVDLGFAPGAWSQVAIDKVTPEGRVIGVDILPANPPPGVSTFQGSMKSPGVRELLKKFLMDPSRGKMRENRYLRKGDVDEEELEEMERGYLDRERDAEREEKFEGVGEDDTRGPVDVVLSDMLMNTSGIPVRDHAYSIDLCYTAMSFAMDVLKTSGVFVCKVYSGDQECELKAKLKLMFSTVYCDKPEASRTESREVYYVSKLLRKGVTKEKVFPGGDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCA0</accession>
    <name>D5GCA0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dynactin subunit 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00000644001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Part of the dynactin complex that activates the molecular motor dynein for ultra-processive transport along microtubules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynactin subunits 5/6 family. Dynactin subunit 6 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430108">
        <property type="protein sequence ID" value="CAZ82143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837952.1">
        <property type="nucleotide sequence ID" value="XM_002837906.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCA0"/>
    <dbReference type="STRING" id="656061.D5GCA0"/>
    <dbReference type="GeneID" id="9184835"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000644001"/>
    <dbReference type="eggNOG" id="ENOG502S7GK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085418_2_1_1"/>
    <dbReference type="InParanoid" id="D5GCA0"/>
    <dbReference type="OMA" id="ITMQAET"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005869">
        <property type="term" value="C:dynactin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070840">
        <property type="term" value="F:dynein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027777">
        <property type="entry name" value="DCTN6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13072">
        <property type="entry name" value="DYNACTIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13072:SF0">
        <property type="entry name" value="DYNACTIN SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ82143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="171" mass="17881" checksum="C8067F7A8C74A367" modified="2010-06-15" version="1">MPPKPRPSEAVAKPPTVLASTVVIAETASLTGTFPIKIGANTVIHPRAKLASAHGPITIGEHCIICERTQICPPDAEGLIIGDGVVVEVNSVVEGSVVGAGSVVEVGLNCKLGPLTKVLEGEVVEDNTVIFGSGQRRIDASGTADARRRLLERHIESLRVLIPSNPSKFIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCA5</accession>
    <name>D5GCA5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00000650001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430108">
        <property type="protein sequence ID" value="CAZ82148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837957.1">
        <property type="nucleotide sequence ID" value="XM_002837911.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCA5"/>
    <dbReference type="GeneID" id="9184826"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000650001"/>
    <dbReference type="HOGENOM" id="CLU_2484957_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCA5"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ82148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="87" mass="10121" checksum="88F8DB7BC614A67B" modified="2010-06-15" version="1">MPIGTNHEITADSIKRHYTIVTTLNRKRRNPDRVRNYICQSVRDIFLMKYIPHLGLVFNRRCPDSSDQALALPLASYLYKLVGTEML</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCG5</accession>
    <name>D5GCG5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DNA replication licensing factor MCM3</fullName>
            <ecNumber evidence="11">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00005881001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the MCM2-7 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the MCM family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="11"/>
    <dbReference type="EMBL" id="FN430109">
        <property type="protein sequence ID" value="CAZ82208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838017.1">
        <property type="nucleotide sequence ID" value="XM_002837971.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCG5"/>
    <dbReference type="FunCoup" id="D5GCG5">
        <property type="interactions" value="962"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GCG5"/>
    <dbReference type="GeneID" id="9186390"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005881001"/>
    <dbReference type="eggNOG" id="KOG0479">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000995_6_1_1"/>
    <dbReference type="InParanoid" id="D5GCG5"/>
    <dbReference type="OMA" id="ECILECD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071162">
        <property type="term" value="C:CMG complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005656">
        <property type="term" value="C:nuclear pre-replicative complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904931">
        <property type="term" value="F:MCM complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902975">
        <property type="term" value="P:mitotic DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006267">
        <property type="term" value="P:pre-replicative complex assembly involved in nuclear cell cycle DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006279">
        <property type="term" value="P:premeiotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030466">
        <property type="term" value="P:silent mating-type cassette heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17754">
        <property type="entry name" value="MCM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000234">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.10">
        <property type="entry name" value="mini-chromosome maintenance (MCM) complex, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008046">
        <property type="entry name" value="Mcm3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056575">
        <property type="entry name" value="WH_MCM3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF46">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF14551">
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    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF23191">
        <property type="entry name" value="WHD_MCM3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01659">
        <property type="entry name" value="MCMPROTEIN3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 11" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 11" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="13">
        <location>
            <begin position="246"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="565"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="576"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="598"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="625"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="652"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="678"/>
            <end position="693"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ82208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="772" mass="85729" checksum="CDAE55FCB4A5C4C5" modified="2010-06-15" version="1">MERDPITDQVFKDRVRQFSEFLDPNDEDQRSYTDSIKLMLNRGQRRLQVNLDEIRNHNRELADGLLNTPFDYLPTLDRALTEVASTLRDRARHEEITDDTLFYCALTGSFGEYAVNPRTLSSKHLNHMISLEGIVTRCSLVRPKVVKSVHYNENKKVFVGRTYKDQTMSYGYSVYRDHQTISIQEMPERAPAGQLPRSVDVIMDDDLVDRVKPGDRIQLVGLYRSLGNRNAGQGSSTFRTLLIANNSLAPSIYGHDHIKRAILLMLLGGMEKNLDNGTHLRGDINILMVGDPSTAKSQLLRFVLNTAPLAIATTGRGSSGVGLTAAVSSDKETGERRLEAGAMVLADRGVVCIDEFDKMSDIDRVAIHEVMEQQTVTIAKAGIHTSLNARCSVIAAANPIYGQYDPHKDPHRNIALPDSLLSRFDLLFVVTDDIEDFRDRQVSEHVLRMHRYRRPGTEEGAPIRENPGQMLGKYIQYAKTRIKPVLSKGAADIIVATYSALRNDELEGSQRRTSPMTARTLETLIRLATAHAKARLSNRVEERDAIAAEEILRFALFKEIVQDERKKRRRVGSAEMDVDSSDDDDDDEEEESQPRTRGSSRRARASARNKSQTANGGADDHEMDAAVDDDDDDDLYSAPTSSRHTRRSGRSAVTQDESQNSWASSHRASSVLQSQTESGQASSSAGPSAAAESQSEAISPARVLVFQRALAGLLNAPVFQDDAAEVGPLLVEINNSIGQEQRFSRGEAEAALEKMNDGNKIMFTGGDIVYKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCL4</accession>
    <name>D5GCL4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00000711001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430114">
        <property type="protein sequence ID" value="CAZ82257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838066.1">
        <property type="nucleotide sequence ID" value="XM_002838020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCL4"/>
    <dbReference type="FunCoup" id="D5GCL4">
        <property type="interactions" value="62"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GCL4"/>
    <dbReference type="GeneID" id="9187876"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000711001"/>
    <dbReference type="eggNOG" id="ENOG502SC94">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_174950_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCL4"/>
    <dbReference type="OMA" id="KPMWPFY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006995">
        <property type="entry name" value="ATP_synth_F0_jsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28060">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT J, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28060:SF1">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT J, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04911">
        <property type="entry name" value="ATP-synt_J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ82257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="66" mass="7345" checksum="9DF8CDFCDAAA6BF8" modified="2010-06-15" version="1">MLERIFGKKWPTPIAKPMAPFYIAGVVVFYGVNSLSNFLANTEEYKNDPRNPNARANPSGNAGQHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDM6</accession>
    <name>D5GDM6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00001122001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430152">
        <property type="protein sequence ID" value="CAZ82619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDM6"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001122001"/>
    <dbReference type="HOGENOM" id="CLU_3415271_0_0_1"/>
    <dbReference type="InParanoid" id="D5GDM6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="27" mass="3115" checksum="4BBF7115301F1E64" modified="2010-06-15" version="1">MLGFPTRGVVRVMALFYLPMFVFSMFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDV1</accession>
    <name>D5GDV1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00006284001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430153">
        <property type="protein sequence ID" value="CAZ82694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838503.1">
        <property type="nucleotide sequence ID" value="XM_002838457.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDV1"/>
    <dbReference type="STRING" id="656061.D5GDV1"/>
    <dbReference type="GeneID" id="9184489"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006284001"/>
    <dbReference type="HOGENOM" id="CLU_1788225_0_0_1"/>
    <dbReference type="InParanoid" id="D5GDV1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ82694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="145" mass="16807" checksum="0BCFAFFE82412AD5" modified="2010-06-15" version="1">MLRPEWHDSGPEELLLMHARVYALANYWAIEELSELALNRLLFNLQALKATEYNPVQVRYIVELISYVYEKTCMRHGEREPMRQGVTRFTALELTKLDSEGEIARLMGTYGDFADDLLSDLTRRIKLAEVWGGTQHRYLAGIEVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>D5GE04</accession>
    <name>D5GE04_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00001161001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430157">
        <property type="protein sequence ID" value="CAZ82747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838556.1">
        <property type="nucleotide sequence ID" value="XM_002838510.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GE04"/>
    <dbReference type="GeneID" id="9183079"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001161001"/>
    <dbReference type="HOGENOM" id="CLU_880524_0_0_1"/>
    <dbReference type="InParanoid" id="D5GE04"/>
    <dbReference type="OMA" id="DAGDICI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072084" evidence="2">
        <location>
            <begin position="21"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="214"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="214"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ82747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="316" mass="32715" checksum="62965CF08C4938F9" modified="2010-06-15" version="1">MKNLAQLCFLAALAVVPSLAADLGPQFQPDFQPMDKPNFEEFYPGIVPATNVPGLGKRQGSCPTANSFLCLNTGRCCPVGYTCNGDTVNGWCCPIGEFCDSSFTFCPAGQTDCTNLIGVSGCCPTATHRCSNSTGTPGCARLSGSNTPTNICDPGTFMCADGSGCCPDGTRCVAGTTLCRKPCDPGLIECPSGGCCELGFACDDAAGLCRRTLSSGSSIRPSSTRAATIPTLENPFTSISARPTSTVEPASPTGDENPQTDLGPLDTDPSRSRSRGPTPTPRVATTSGGNVLKPGGWLGEWLGGFGTVFFLVFFHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GE34</accession>
    <name>D5GE34_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00006354001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430164">
        <property type="protein sequence ID" value="CAZ82777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838586.1">
        <property type="nucleotide sequence ID" value="XM_002838540.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GE34"/>
    <dbReference type="GeneID" id="9183109"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006354001"/>
    <dbReference type="HOGENOM" id="CLU_2484959_0_0_1"/>
    <dbReference type="InParanoid" id="D5GE34"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ82777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="87" mass="9770" checksum="12B71D3C21A732A1" modified="2010-06-15" version="1">MGSHKRNLTAYKYCTVLPVTPLPTATLRYSRRTARGEGFIQDPKSLTLAPESAPRFGSLPINSLLGLVLRFISFFSSFPFDREYDAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEJ9</accession>
    <name>D5GEJ9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sugar phosphate phosphatase</fullName>
            <ecNumber evidence="7">3.1.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00006527001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Metal-dependent phosphatase that shows phosphatase activity against several substrates, including fructose-1-phosphate and fructose-6-phosphate. Its preference for fructose-1-phosphate, a strong glycating agent that causes DNA damage rather than a canonical yeast metabolite, suggests a damage-control function in hexose phosphate metabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>beta-D-fructose 1-phosphate + H2O = D-fructose + phosphate</text>
            <dbReference type="Rhea" id="RHEA:35603"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:37721"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:138881"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>beta-D-fructose 6-phosphate = dihydroxyacetone + D-glyceraldehyde 3-phosphate</text>
            <dbReference type="Rhea" id="RHEA:28002"/>
            <dbReference type="ChEBI" id="CHEBI:16016"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="7">
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </cofactor>
    </comment>
    <comment type="domain">
        <text evidence="7">Subfamily III proteins have a conserved RTxK motif about 40-50 residues from the C-terminus; the threonine may be replaced by serine or cysteine.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the damage-control phosphatase family. Sugar phosphate phosphatase III subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.-" evidence="7"/>
    <dbReference type="EMBL" id="FN430175">
        <property type="protein sequence ID" value="CAZ82942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838751.1">
        <property type="nucleotide sequence ID" value="XM_002838705.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEJ9"/>
    <dbReference type="FunCoup" id="D5GEJ9">
        <property type="interactions" value="585"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GEJ9"/>
    <dbReference type="GeneID" id="9181572"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006527001"/>
    <dbReference type="eggNOG" id="KOG3870">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030117_2_1_1"/>
    <dbReference type="InParanoid" id="D5GEJ9"/>
    <dbReference type="OMA" id="IFARQKM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097023">
        <property type="term" value="F:fructose 6-phosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103026">
        <property type="term" value="F:fructose-1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.930.60:FF:000002">
        <property type="entry name" value="Protein-glutamate O-methyltransferase C1393.13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10880">
        <property type="entry name" value="Uncharacterised protein PF01937, DUF89, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036075">
        <property type="entry name" value="ARMT-1-like_metal-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039763">
        <property type="entry name" value="ARMT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002791">
        <property type="entry name" value="ARMT1-like_metal-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12260">
        <property type="entry name" value="DAMAGE-CONTROL PHOSPHATASE ARMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12260:SF6">
        <property type="entry name" value="DAMAGE-CONTROL PHOSPHATASE ARMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01937">
        <property type="entry name" value="ARMT1-like_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111321">
        <property type="entry name" value="AF1104-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 7" id="KW-0464">Manganese</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Damage-control phosphatase ARMT1-like metal-binding" evidence="8">
        <location>
            <begin position="23"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ82942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="449" mass="51139" checksum="37ABAA9611312C24" modified="2010-06-15" version="1">MDPGLPKEPYRISDKNSFAYVSARDRWPVILTQAVDDVFKHGDSEDSNHRHEGSKIIRQIAQLKFELQHDRLIRPLEDDGGDDIKGYNEELEKLGNPKWFDAPWLFAECYLYRRLNTFFTNSKTWKTHDVFHVQKATAFRSSRAAVLELASRYRQLVSSLKNSEQPTGTKEATEEGEKKLFTDMCEICLWGNATDLSLLTSLTYADLQQLQGSRARKAASKYILDSQVDKVYELLKKQRDEGKVGRVDIVLDNSGFELFVDLIFGGYLLTAGFATEIVFHPKSLPWFVSDVLPGDFTDLINALRDPESDLKFLFSEWSELHTNGKFLLRPNRFWTTAASYWRLPSAAPRLFEDLKQSELVIFKGDLNYRKLTADAAWDPTTDFSEAIGELGKLGSGVRVLALRTCKADVVVGLSEGKDLALRSGDPEEGKRKWAWSGKWAVIQFWDGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEM8</accession>
    <name>D5GEM8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GDP-mannose transporter</fullName>
            <shortName evidence="7">GMT</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00001248001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">Involved in the import of GDP-mannose from the cytoplasm into the Golgi lumen.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 7">Homooligomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Golgi apparatus membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Cytoplasmic vesicle membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Endoplasmic reticulum membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the TPT transporter family. SLC35D subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430177">
        <property type="protein sequence ID" value="CAZ82971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838780.1">
        <property type="nucleotide sequence ID" value="XM_002838734.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEM8"/>
    <dbReference type="GeneID" id="9184884"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001248001"/>
    <dbReference type="eggNOG" id="KOG1441">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048347_3_0_1"/>
    <dbReference type="InParanoid" id="D5GEM8"/>
    <dbReference type="OMA" id="WMVVNTL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="75"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="137"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="194"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="232"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="262"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="292"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="317"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="352"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="9">
        <location>
            <begin position="229"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="21"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ82971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="376" mass="40481" checksum="464435343C6E577A" modified="2010-06-15" version="1">MPGPSNNNSINIELKSDLEAQKANLLSQQEPDDSDDGLSIGGSTRSSLSGHELKPVILPDSAVKSNSQKSSQTSFLIWTAVNTLATIGIVFTNKRIFDDPNFKNMQTSFAAFHFVCTSLTLFVISRPSFGFFVPKRCGIVEILPLAFAMCFNVILPNLSLAYSSITFYQIARILLTPFVALINLVFYRVSIPTYAALSLIPVCTGVGVVSYYDTRAATPEQAGKVTTVAGVIFAFSGVVASSLYTVWIGTYHKKLNMSSMQLLFNQAPASTFLLLFFIPFADAIPVFGDVPISRWAMILMSGLFASLINLSQFFIIAGAGAVSSTVVGHAKTCSIVILGWMASGRSVSDKSLLGIVLAIGGIIMYSVSVIKARRMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEN1</accession>
    <name>D5GEN1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00001310001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430180">
        <property type="protein sequence ID" value="CAZ82974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838783.1">
        <property type="nucleotide sequence ID" value="XM_002838737.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEN1"/>
    <dbReference type="FunCoup" id="D5GEN1">
        <property type="interactions" value="16"/>
    </dbReference>
    <dbReference type="GeneID" id="9184892"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001310001"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_30_3_1"/>
    <dbReference type="InParanoid" id="D5GEN1"/>
    <dbReference type="OMA" id="HNMAMLL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015793">
        <property type="term" value="P:glycerol transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000061">
        <property type="entry name" value="MFS sugar transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF69">
        <property type="entry name" value="SUGAR TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="113"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="148"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="179"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="280"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="310"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="339"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="373"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="404"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="433"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="16"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ82974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="523" mass="57370" checksum="F0A6191C27739F1D" modified="2010-06-15" version="1">MTKFLGLRGERLGFAVSFVCTTGFLLFGYDQGVMSGIITSPEFNSAIPETKGNSTMQGFVTAIYEIGCLFGAVVVLFAGEAMGRRKAIISGASVMILGVIIQVTAYVGHQSLAQFVIGRIVTGIGNGMNTSSIPTYQAECAKTKNRGLLICIEGGSIAFGTLISYWLNYGVSFAGGDMVWRLPISFQVVFALIIIAGMLVLPESPRWLLSKDRDEEGTFVIAALNGEAVDSPKTQLEKRVILDSMMATGEIGNKSSFRDLLTNGKTQHLRRVILGASSQLMQQIGGCNAVIYYFPILFESSIGQSRNMSLIMGGVNMVVYAIFATTSWFIIERAGRRKLFLWGTIGQMSAMAITFICLIPGTQTAARGAAVGLFLYIASFGATWLPLPWLYPAEVNPLRTRGKANAVSTITNWLFNFVVVMITPVMIDRIGWGTYLFFAVVNACFLPFIYLFYPETRLRSLEEIDFIFAKGYEEKISYVKAAKELPPLSPEEIEEYAIHYGFAHASAPEKPTDLLVDEDRKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>D5GF46</accession>
    <name>D5GF46_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00001827001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430200">
        <property type="protein sequence ID" value="CAZ83139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838948.1">
        <property type="nucleotide sequence ID" value="XM_002838902.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GF46"/>
    <dbReference type="STRING" id="656061.D5GF46"/>
    <dbReference type="GeneID" id="9188000"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001827001"/>
    <dbReference type="HOGENOM" id="CLU_2160248_0_0_1"/>
    <dbReference type="InParanoid" id="D5GF46"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14361">
        <property type="entry name" value="UBA_HYPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038922">
        <property type="entry name" value="HYPK_UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044034">
        <property type="entry name" value="NAC-like_UBA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19026">
        <property type="entry name" value="UBA_HYPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nascent polypeptide-associated complex subunit alpha-like UBA" evidence="2">
        <location>
            <begin position="70"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ83139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="111" mass="11745" checksum="DDF01D3D8400843A" modified="2010-06-15" version="1">MTELETTQDRVAPSNAERCVKAMKAMDNEGDTPVEQVTQESAEALGKAMQGLSVQQAPTQKAAPPPSSRVNAAGVTLIMEEMEVTKAKATDALRGCGGNVGEALRKLMHAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFB5</accession>
    <name>D5GFB5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00006811001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430208">
        <property type="protein sequence ID" value="CAZ83208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839017.1">
        <property type="nucleotide sequence ID" value="XM_002838971.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFB5"/>
    <dbReference type="FunCoup" id="D5GFB5">
        <property type="interactions" value="246"/>
    </dbReference>
    <dbReference type="GeneID" id="9188064"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006811001"/>
    <dbReference type="eggNOG" id="ENOG502QRXN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033165_0_0_1"/>
    <dbReference type="InParanoid" id="D5GFB5"/>
    <dbReference type="OMA" id="GWGYMLM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000191">
        <property type="entry name" value="Retrograde regulation protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.150">
        <property type="entry name" value="Exopolyphosphatase. Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050273">
        <property type="entry name" value="GppA/Ppx_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003695">
        <property type="entry name" value="Ppx_GppA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057512">
        <property type="entry name" value="RTG2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30005">
        <property type="entry name" value="EXOPOLYPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30005:SF0">
        <property type="entry name" value="RETROGRADE REGULATION PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02541">
        <property type="entry name" value="Ppx-GppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23566">
        <property type="entry name" value="RTG2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ppx/GppA phosphatase N-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="RTG2 C-terminal" evidence="2">
        <location>
            <begin position="308"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ83208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="512" mass="55904" checksum="ACFED7C5997E7967" modified="2010-06-15" version="1">MPTVFQDRAAISLYDAQFSGTTKSPISKEVIGQILASMRRFKSVCSDFGVPDQNVRVVATEATREAQNSAEFRDRITEVSGWDVELLSKSEEARIGAEGVASSVGCVTGLVMDLGGGSTQLGWMRSEKGVCEMAEKPVSMPYGAAALTKRIETAIDKRAIGLLRDEMKERIAEAFTSLQIPEDLQLDAEAEGGFTMYLSGGGFRGFGYLLLDQHDIKPYPVPIINGFKAPGNAFSSLADLHLDPELSDRLGEKFRISGRRARQIPAVAFLINTLIETLPKIKTVIFCQGGVREGALFEKLPREIRAQDPLHVATLPFAPRSTVPVSLLLSYALPRSAPYIIRFETAPALSNMLIYHSNVPKESRASCGLHSTTTGILASAHGLTHETRALLALSLCERWGGEVSDSNLKTRLQDLVGPELAYWARYLGAIARVVGNVYPAGIIGEEKLRFFATDEMANTGAIMLKVAIREDDPATSAVMVMDSIKNLAKVGKKRNREGFRRKVALNLVRDLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFB7</accession>
    <name>D5GFB7_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Anaphase-promoting complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00006814001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the cullin family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430208">
        <property type="protein sequence ID" value="CAZ83210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839019.1">
        <property type="nucleotide sequence ID" value="XM_002838973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFB7"/>
    <dbReference type="FunCoup" id="D5GFB7">
        <property type="interactions" value="585"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GFB7"/>
    <dbReference type="GeneID" id="9188066"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006814001"/>
    <dbReference type="eggNOG" id="KOG2165">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007149_4_2_1"/>
    <dbReference type="InParanoid" id="D5GFB7"/>
    <dbReference type="OMA" id="TYFMYET"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007091">
        <property type="term" value="P:metaphase/anaphase transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070979">
        <property type="term" value="P:protein K11-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1310.10">
        <property type="entry name" value="Cullin Repeats"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.130">
        <property type="entry name" value="Cullin, Chain C, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044554">
        <property type="entry name" value="ANAPC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014786">
        <property type="entry name" value="ANAPC2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059120">
        <property type="entry name" value="Cullin-like_AB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016158">
        <property type="entry name" value="Cullin_homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036317">
        <property type="entry name" value="Cullin_homology_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057975">
        <property type="entry name" value="TPR_ANAPC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45957">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45957:SF1">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08672">
        <property type="entry name" value="ANAPC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26557">
        <property type="entry name" value="Cullin_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25773">
        <property type="entry name" value="TPR_ANAPC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01013">
        <property type="entry name" value="APC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00182">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75632">
        <property type="entry name" value="Cullin homology domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50069">
        <property type="entry name" value="CULLIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0498">Mitosis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Cullin family profile" evidence="7">
        <location>
            <begin position="255"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00016068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ83210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="565" mass="64100" checksum="741D729969392C83" modified="2010-06-15" version="1">MQVINTLLTTYINAHFAGVWETPSKVGDDLNHWVEHVLGQLVLDVLFSSKPPNSSEGVGEDDIKRRKEDLESWKRIAVGRLGRLRVGELFDVVVEWPESLGGVEDLKSYIISPQTRLHLTTTFTSALSTRLLHPAAATSDILRAYISLIRAFTVLDPRGVLLDRVSRGIRRYLRERDDTVRVIVRGIMSESLPDGLEDAEDLSELAHELGKGIPATQQGGGLEELDYDDLSWVPDPVDAGPEFRRSKGLDVVGSLISLWESREVWTKEVAAVLSSRLLGSDEWLFEREIRTIELLKLRLGETTMQGCEVMLKDIADSKRSDAHIRAEEHLEIEGFPEIHTKILSRLFWPTMKEGGFQVPLEIKELQERYERGFENLKKKRKLTWLQTMGTVEVELELEDRVVQFPDATTWQAAVIHQFDEEGLSDQWTIPQLADNLEMEESLVKKAVQFWQSRDILREGSNGVYAVIENLEELEANTPLHTGVTAAEEEEEVTDEKKRERMIVEQFVVGMLTNGGAMPLERISVMLGLVPGGFSWGQEELREFLSRMKSEGKVEYEGGSWRIPKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFN8</accession>
    <name>D5GFN8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00001869001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TIP41 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430210">
        <property type="protein sequence ID" value="CAZ83331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839140.1">
        <property type="nucleotide sequence ID" value="XM_002839094.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFN8"/>
    <dbReference type="FunCoup" id="D5GFN8">
        <property type="interactions" value="770"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GFN8"/>
    <dbReference type="GeneID" id="9188281"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001869001"/>
    <dbReference type="eggNOG" id="KOG3224">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039187_0_1_1"/>
    <dbReference type="InParanoid" id="D5GFN8"/>
    <dbReference type="OMA" id="DMILFED"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031929">
        <property type="term" value="P:TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051330">
        <property type="entry name" value="Phosphatase_reg/MetRdx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007303">
        <property type="entry name" value="TIP41-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21021">
        <property type="entry name" value="GAF/PUTATIVE CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21021:SF16">
        <property type="entry name" value="TIP41-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04176">
        <property type="entry name" value="TIP41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="269" mass="31148" checksum="F93EF3D0AD53845A" modified="2010-06-15" version="1">MSRSPPLQTAPNIDVGKSITRGDWKVTTHKRPILNSTEIETMTTTLGISPPEMIFGNNSVEVTNERLNWSISFNALDALELVDKSGESMLKVSYSEAWQRMRERVHEDIKEIVKPYDWTYTTNYQGTVHNPDRPFQQTGSEIPFEKLKRPDPILLFDDVMLYEDELADNGISILSVKIRVMPERLLILQRFFMRLDGVLFRLRDTRLFIEFETGEIVKQYQAREDTYANVRSGLYMHPPEQIPSLLRDDGFIAERLPVIGIERERVLVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGV3</accession>
    <name>D5GGV3_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Dipeptidyl-peptidase V</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00002049001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S9C family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430276">
        <property type="protein sequence ID" value="CAZ83746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839555.1">
        <property type="nucleotide sequence ID" value="XM_002839509.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGV3"/>
    <dbReference type="GeneID" id="9181717"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002049001"/>
    <dbReference type="eggNOG" id="KOG2100">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008615_0_1_1"/>
    <dbReference type="InParanoid" id="D5GGV3"/>
    <dbReference type="OMA" id="YKHWDEW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000028">
        <property type="entry name" value="S9 family peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42776:SF11">
        <property type="entry name" value="DIPEPTIDYL-PEPTIDASE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42776">
        <property type="entry name" value="SERINE PEPTIDASE S9 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="11">
        <location>
            <begin position="481"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="269"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ83746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="710" mass="79061" checksum="FC8275AE23B09E6D" modified="2010-06-15" version="1">MLVSFCSPSWYWGNLLQSKDLLSAPRRGEAVPNKSGSLAIFHLSSYSFDTHSSSSSWNLLNLGDGDTTILFSDPTVQEVIWLDDERLLYINGTNSDIKGGCELWITKHSKPSEGYKAASLPASVSNLKAVSPSDKSVKFVVSALAYPNGTIYNPESAPKKLDTGLTYETLFVRHWDVYDKPEKSSLFSGVLSFDDDKFKLEKEIKNLMANADGTIEAPIAPFGGADDFDVSPDGRTVAFVSKTPYLNPANYTQVLVYIVPFDGSSKPKPINIEGKDTDGNPLPEGAASSPKFSPNGGALAYLQMYINGYESDQNNLFIYMMDSSSITALWKPWDCSPSGLLWSGDANTIYLTAESYGRVRIFEARINDDTPKEITAEGNIVALHLLPSGDFLISRNSLTSSIGYFVFNPSSKRFKTLLLPTKVDPQLADLSKITVEDFSFDGAETKVHGFIVKPSNFDPSKKTRLAFLIHGGPQGAWTDSWSTRWNPALFAEAEYIVVTVNPTGSTGYGQAFTDAIQNQWGGLPYIDLVKSMEYLENAEEYNYIDFNRAVALGASYGGYMINYIQGMPLGKKFQALVSHDGSFAPQNIYSTDELYFPQREFNGTLWDDPENYSKWSPSSHIKNWGTPQFVIHNALDFRLPISEGLSVFHILQSLGIPSKFLTFPDENHWVLKPENSLVWHREVLEWINRWTETRASRGTTQTVLKTREKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>D5GH60</accession>
    <name>D5GH60_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pescadillo homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Nucleolar protein 7 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">NOP7</name>
        <name evidence="7" type="ORF">GSTUM_00007762001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the NOP7 complex, which is required for maturation of the 25S and 5.8S ribosomal RNAs and formation of the 60S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the NOP7 complex, composed of ERB1, NOP7 and YTM1. Within the NOP7 complex ERB1 appears to interact directly with NOP7 and YTM1. The NOP7 complex also associates with the 66S pre-ribosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
            <location evidence="4">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
            <location evidence="4">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the pescadillo family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430308">
        <property type="protein sequence ID" value="CAZ83885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839694.1">
        <property type="nucleotide sequence ID" value="XM_002839648.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GH60"/>
    <dbReference type="FunCoup" id="D5GH60">
        <property type="interactions" value="1224"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GH60"/>
    <dbReference type="GeneID" id="9182590"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007762001"/>
    <dbReference type="eggNOG" id="KOG2481">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019619_1_1_1"/>
    <dbReference type="InParanoid" id="D5GH60"/>
    <dbReference type="OMA" id="QKVTWIV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070545">
        <property type="term" value="C:PeBoW complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043021">
        <property type="term" value="F:ribonucleoprotein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17709">
        <property type="entry name" value="BRCT_pescadillo_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03028">
        <property type="entry name" value="Pescadillo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010613">
        <property type="entry name" value="PES"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12221">
        <property type="entry name" value="PESCADILLO - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12221:SF6">
        <property type="entry name" value="PESCADILLO HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06732">
        <property type="entry name" value="Pescadillo_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="2 4" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="BRCT" evidence="6">
        <location>
            <begin position="295"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="353"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="443"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="470"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="480"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="560"/>
            <end position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ83885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="593" mass="66678" checksum="98415016A85A9346" modified="2010-06-15" version="1">MPKIKKKGVSGNAKNFITRTQAVRKLQVSLPDFRRLCIFKGIYPREPRNKKKANKGSTQSTTFYYTKDIQYLLHEPVLQKFRDHKAFAKKLQKALGRGDFSDAKRLETTQKPVVRLDHIVKERYPTFTDALRDLDDALSMIHLFASLPTNENLPPATVVNCARLTKEWAHYVIRAGALRKSFLSIKGIYYQAEVGGGQEVLWLVPYSFAQDVPIDVDFRIMHTFVEFYTTLVGFALFRLYAIEGLVYPPRFDKDMDENAEAGERKDVEDLTIDTFTPAAASDQLPQPTPSQTTSTSTALFSGTTFYLSRETPREPLEFLLRSFGCKRIGWDAVLGAGAYTENSSDTSITHQIVDRPSTFPSPPPPSAAGGDNAPSTNAAATGRIPGRIYIQPQYVWDCINSGRLLPPGEYAPGALLPPHLSPWVKPGEGYDPTAALPDVEHNEEVEGSDNGMQEDLASDSEPEPEPVLPLPTTVTPIEGESSEEEEEEESEKNYQRELATEAAGAGLPAPSEQDMKKQKKKNKEKMSRQQREEEDQKEMKKIMMSRRKRKLYEKMLYSNAKKESEVEGLKRKRRRLEGERSKGKKGGKGSEEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>D5GH73</accession>
    <name>D5GH73_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">mRNA 3'-end-processing protein RNA14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00007778001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 4">Component of the cleavage factor IA (CFIA) complex, which is involved in the endonucleolytic cleavage during polyadenylation-dependent pre-mRNA 3'-end formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="4">Nucleus and/or cytoplasm.</text>
    </comment>
    <dbReference type="EMBL" id="FN430308">
        <property type="protein sequence ID" value="CAZ83898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002839661.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GH73"/>
    <dbReference type="FunCoup" id="D5GH73">
        <property type="interactions" value="1147"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GH73"/>
    <dbReference type="GeneID" id="9182603"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007778001"/>
    <dbReference type="eggNOG" id="KOG1914">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007630_1_1_1"/>
    <dbReference type="InParanoid" id="D5GH73"/>
    <dbReference type="OMA" id="VQLWSVY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0180010">
        <property type="term" value="P:co-transcriptional mRNA 3'-end processing, cleavage and polyadenylation pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045243">
        <property type="entry name" value="Rna14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008847">
        <property type="entry name" value="Suf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19980:SF0">
        <property type="entry name" value="CLEAVAGE STIMULATION FACTOR SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19980">
        <property type="entry name" value="RNA CLEAVAGE STIMULATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05843">
        <property type="entry name" value="Suf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Suppressor of forked" evidence="6">
        <location>
            <begin position="212"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="597"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="822"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="972"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="48"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="124"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="150"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="835"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="902"/>
            <end position="922"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU369035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ83898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="991" mass="111181" checksum="F9DF1020CBADD7AA" modified="2010-06-15" version="1">MAEHNNSDDADAAFLNALKAEQEMAEAGQKASELGGESELIGNEPEEGAEKEATFRELSDQVEKDNHLQINESKDTSTAASGATSLLPGVAVASPDGGPSSVNGSAMGDPAAQPLLNANMGAGPSTQESSAISSPSVTPVGALVPDENNNDSAPHTLSVTTPTAGSNNNDSLPVSSTTPVNAPSTAVPVQSPVQKGNANVANKRKRLPQDIVGQLEDRIAEDPRGDIDAWLALIEEHRKKGKFDDVRAVYERFFVVFPAAADQWISYVKMELANNELQHVERIFQRSLFNVPNVELWSMYLDYIRRRNNLTTDTGGKARAVVNQSYEFVLNNVGCDREAGRIWTEYIQFVKSAPGNVGGSGWQDQQKMDSLRKVYQKAVTMPVQGVEQLWREYDQFEQGLNKLTARKFLQERSPMFMTARGCLIELSNITKGLRRDTLSRLPPAPGYEGYEDYTRQVELWKKWIQWEKGDPLVLAKEDPASLSTRIIYVFKQALMALRFWPEIWFDAAEWCFANGMDDQGVDFLNQGMVANPESCLLHFKYAERLEATTIIEGGEEALIRKGQLVRKPYDDLLNMLYEQVQKIQRKEKEELARIEDAFELTNPRNAEDDDEDDDPTTAATRAAKDAQVATLREGTKAQLQVMTKTISAVWINLMRAMRRVQGHGKVNEALGGSRQIFADARRRGKINSDVYVASALIEYHCYKDPAALKIFERGMRLFPEDENFALEYLKHLVAINDITNARAVFETFVGRVTAEKARKVYEFFYDYEAHYGELGQVYKMEKRMSELYPTDPYISRFSSRYAYRAVDPCSFLAVVSPAQQCKPKSQAISPPVPDHLPRDGRDYRDHPQPPPTAPANGRTVSPKRPANEELSDSSEHQRPRKLHRPDSPLKGAAGRRLDQQKRYQQNQRQPQQHQPLPMQMHQPPAPTQLPDLIMYLLSILPGADKYQAARFRPEAMVGLLGNINLPRDVQANNRESASIHRSVPQGPRRQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHL4</accession>
    <name>D5GHL4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007973001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430319">
        <property type="protein sequence ID" value="CAZ84007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839816.1">
        <property type="nucleotide sequence ID" value="XM_002839770.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHL4"/>
    <dbReference type="GeneID" id="9183661"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007973001"/>
    <dbReference type="HOGENOM" id="CLU_1612013_0_0_1"/>
    <dbReference type="InParanoid" id="D5GHL4"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="165" mass="19166" checksum="B030EE3A12B2882E" modified="2010-06-15" version="1">MVPVNHLSIAPALRHFLPFPLLTVPIKEGQFGEGEREKNPAILWVSEREKKKKKKNHPKLNSSNRFRTLQTKRAGYAKIRPRRKGGLWERTDRNYFRSLEKSNQRTTPCPQNRIDRRLGSPSSTDLSNSLLGGTTQDCAPRMDVRHSGEPPRRPEVRRSRLWLHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHP9</accession>
    <name>D5GHP9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00008084001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430320">
        <property type="protein sequence ID" value="CAZ84079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839888.1">
        <property type="nucleotide sequence ID" value="XM_002839842.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHP9"/>
    <dbReference type="GeneID" id="9184172"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008084001"/>
    <dbReference type="eggNOG" id="ENOG502RF04">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035756_2_0_1"/>
    <dbReference type="InParanoid" id="D5GHP9"/>
    <dbReference type="OMA" id="FICWYYY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd03386">
        <property type="entry name" value="PAP2_Aur1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026841">
        <property type="entry name" value="Aur1/Ipt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052185">
        <property type="entry name" value="IPC_Synthase-Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31310:SF10">
        <property type="entry name" value="INOSITOLPHOSPHOTRANSFERASE AUR1_IPT1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14378">
        <property type="entry name" value="PAP2_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="352"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="377"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="443"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="485"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="510"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="Inositolphosphotransferase Aur1/Ipt1" evidence="7">
        <location>
            <begin position="334"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="Inositolphosphotransferase Aur1/Ipt1" evidence="7">
        <location>
            <begin position="482"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="206"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="207"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ84079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="558" mass="63364" checksum="E1EEA107B24AB1C2" modified="2010-06-15" version="1">MEGAFLAQFQSGGKKQLGVSYFRQTEAHPGRQHATNNPSLGMRWFPALSLLLPFALGGAAEDEELREASSDPHLEGEQCFPRYKPPRSVSSPSLETCLPSPFCNKYTRSILFPSLSPRCGRKKFDFCRKRKGFLVCVVPSGVNASSTPLSSAPPAWNSEPAWKLPEWGEPVIVASILFFACFHTRRRRYNILNNPKSDYRSLLENNGSGEFSPSPSRSSMDGRSSDDEDEDLTVLSTSKHPPKTRSCCCLRILTPNSSRFSNNWHSRIMYKFPFLMEMFYWIITYAIYRVSHILSQELFSDDIWGTAQENGLSVLNAEQYSLLRFFFPVKEIDVQKWFMDGHQELLTFLNKTYALIHIPGSVFFIAWYYYSAPSHAIFAVVRRTVTLCNWMAFIIFAFFPCMPPRLLPPEYGFIDTVRRDDAESVWMSGKFVNHLAAMPSMHFGYSFMIGVTLVYHSGVLSFMGLGYGGRKGKVLQTGVGKWWRIWYCVLGISYPTMILTTIIATANHYWLDACAGALVAAIAFLCNRVFLVFVPLEDLLLWALRLEKPKPNCGQHAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHT3</accession>
    <name>D5GHT3_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">rRNA adenine N(6)-methyltransferase</fullName>
            <ecNumber evidence="6">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00008028001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="6"/>
    <dbReference type="EMBL" id="FN430320">
        <property type="protein sequence ID" value="CAZ84044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839853.1">
        <property type="nucleotide sequence ID" value="XM_002839807.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHT3"/>
    <dbReference type="FunCoup" id="D5GHT3">
        <property type="interactions" value="727"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GHT3"/>
    <dbReference type="GeneID" id="9183698"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008028001"/>
    <dbReference type="eggNOG" id="KOG0820">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041220_2_0_1"/>
    <dbReference type="InParanoid" id="D5GHT3"/>
    <dbReference type="OMA" id="ANYRTWC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000179">
        <property type="term" value="F:rRNA (adenine-N6,N6-)-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.480:FF:000002">
        <property type="entry name" value="rRNA adenine N(6)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001737">
        <property type="entry name" value="KsgA/Erm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020596">
        <property type="entry name" value="rRNA_Ade_Mease_Trfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020598">
        <property type="entry name" value="rRNA_Ade_methylase_Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727">
        <property type="entry name" value="DIMETHYLADENOSINE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727:SF7">
        <property type="entry name" value="DIMETHYLADENOSINE TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00398">
        <property type="entry name" value="RrnaAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00650">
        <property type="entry name" value="rADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01131">
        <property type="entry name" value="RRNA_A_DIMETH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51689">
        <property type="entry name" value="SAM_RNA_A_N6_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="3 5 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 5 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribosomal RNA adenine methylase transferase N-terminal" evidence="8">
        <location>
            <begin position="51"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ84044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="294" mass="32785" checksum="F6972985AECF4FBE" modified="2010-06-15" version="1">MPKAKKQPHPKGNSPYSRTSTTAKEAASRAVNNVFRMNTDLGQHILKNPGVAQAIVNKAGLKQSDVVLEVGPGTGNLTVKILEQARKVIAVEMDPRMAAELTKRVQGKPEQTRVEILLGDVIKTELPYFDVCISNTPYQISSPLVFKLLALSPAPRTCILMFQREFAMRVVAQPGDTLYCRLSVESSVIRIEPKNPRPPISYDEWDGLLRIVFVRKNKTIAAGFKSSAVMALLERNYRTWCATNNVKLEDEKVKGLVDSVLAETELREKRAGKCDEADFLKLLYAFNQQGIHFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>D5GI42</accession>
    <name>D5GI42_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Galactokinase</fullName>
            <ecNumber evidence="3">2.7.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Galactose kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">GSTUM_00008260001</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>alpha-D-galactose + ATP = alpha-D-galactose 1-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13553"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28061"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58336"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:13554"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate metabolism; galactose metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GHMP kinase family. GalK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.6" evidence="3"/>
    <dbReference type="EMBL" id="FN430323">
        <property type="protein sequence ID" value="CAZ84185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839994.1">
        <property type="nucleotide sequence ID" value="XM_002839948.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GI42"/>
    <dbReference type="FunCoup" id="D5GI42">
        <property type="interactions" value="982"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GI42"/>
    <dbReference type="GeneID" id="9181903"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008260001"/>
    <dbReference type="eggNOG" id="KOG0631">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017814_6_2_1"/>
    <dbReference type="InParanoid" id="D5GI42"/>
    <dbReference type="OMA" id="GFHDTYF"/>
    <dbReference type="UniPathway" id="UPA00214"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:galactokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="term" value="P:galactose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
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        <property type="term" value="P:positive regulation of transcription by galactose"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:sterol biosynthetic process"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="GAL1p Galactokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GAL1p Galactokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Galactokinase"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000705">
        <property type="entry name" value="Galactokinase"/>
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    <dbReference type="InterPro" id="IPR019741">
        <property type="entry name" value="Galactokinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019539">
        <property type="entry name" value="GalKase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
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        <property type="entry name" value="GHMP_kinase_N_dom"/>
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        <property type="entry name" value="Mevalonate/galactokinase"/>
    </dbReference>
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        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
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        <property type="entry name" value="gal_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10457:SF7">
        <property type="entry name" value="GALACTOKINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10457">
        <property type="entry name" value="MEVALONATE KINASE/GALACTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GalKase_gal_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000530">
        <property type="entry name" value="Galactokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00473">
        <property type="entry name" value="GALCTOKINASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00959">
        <property type="entry name" value="MEVGALKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00106">
        <property type="entry name" value="GALACTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00627">
        <property type="entry name" value="GHMP_KINASES_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="10" id="KW-0299">Galactose metabolism</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="9" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="11" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
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        <location>
            <begin position="32"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="16">
        <location>
            <begin position="129"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase C-terminal" evidence="17">
        <location>
            <begin position="404"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004947"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012315"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00019487"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023144"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00029590"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00049538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF10509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAZ84185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="506" mass="55369" checksum="5D4337B1E58E572F" modified="2010-06-15" version="1">MSEPVPEVLTIEEIYPPEFGAAQKQRWEHLLAKFEERYGAPADYVARSPGRVNIIGEHIDYMLFSVLPMAVNSDFLLAVRAIPNTNTIKIANILERYPHREFQVPAEGELEIDAAQLEWSNYFRAGLRGALELLRRKGLSPKNVGMEILADGNVPPGAGLSSSAAFTCSSALASLTAMGDGTVDKKELVNLAVVSERYVGVNSGGQVLCSVFPGDQGSALYISFHPTLDAIPVAFPETTPELTFVIADTLVAADKHTTGQINYNLRVVECTLAAQILAKKLNLGQLPEDAGPLGNSFKGLMDKYFLGKDLPLDKKLEKLIEDTREYLGKKGYTREEIAETLDLTVGELVQRCMTKFPILASHFQLGSRALHVLQEAHRVVTFKALLDSCTASPPYPTDTKIPIQLGAIMNESHESCKNLYNCSCPELDTLCEIARSAGSYGSRLTGAGWGGCSVHLVPQDKVHAVKEAWKREYYEKKFPGISKERVENAIVASKPGSGAALFRVRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIC2</accession>
    <name>D5GIC2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00008420001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430326">
        <property type="protein sequence ID" value="CAZ84265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="tml:GSTUM_00008420001"/>
    <dbReference type="HOGENOM" id="CLU_3435094_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIC2"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ84265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="14" mass="1666" checksum="1FC37C81C9FF9E3B" modified="2010-06-15" version="1">MENRGQYLTDPITQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>D5GID3</accession>
    <name>D5GID3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008434001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430326">
        <property type="protein sequence ID" value="CAZ84276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840085.1">
        <property type="nucleotide sequence ID" value="XM_002840039.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GID3"/>
    <dbReference type="GeneID" id="9184634"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008434001"/>
    <dbReference type="HOGENOM" id="CLU_2672898_0_0_1"/>
    <dbReference type="InParanoid" id="D5GID3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="65"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="75" mass="8375" checksum="1E104F4423C43B92" modified="2010-06-15" version="1">MAVGYHDPHRYKEEPASKVGEREQDKTDVNKSGPGKEWGGGVKGGGTKCMMNWMDGQMETPPPHLTHFTHPMNPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIE1</accession>
    <name>D5GIE1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008445001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430326">
        <property type="protein sequence ID" value="CAZ84284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840093.1">
        <property type="nucleotide sequence ID" value="XM_002840047.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIE1"/>
    <dbReference type="GeneID" id="9184642"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008445001"/>
    <dbReference type="eggNOG" id="ENOG502S7T3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105491_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIE1"/>
    <dbReference type="OMA" id="FESETGP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42115">
        <property type="entry name" value="BETA-SYNTHASE (BETA-THIONASE), PUTATIVE (AFU_ORTHOLOGUE AFUA_3G08420)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42115:SF1">
        <property type="entry name" value="BETA-SYNTHASE (BETA-THIONASE), PUTATIVE (AFU_ORTHOLOGUE AFUA_3G08420)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="1">
        <location>
            <begin position="100"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="149" mass="16426" checksum="D513D86A0B0126B5" modified="2010-06-15" version="1">MTYSPSAKYRGATVEDLDLPPALSISPSAPISTALAMAHDRDYSQLTVTSSTTRALLGYLSVPKIESLLQSSSPGSNIRLSDPVSKVMNRFDRKKGNVYKVITPNTTLEELEEFLKKEEFAVITDEGRKFVLGVATRGDLEEFTKRRPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJI3</accession>
    <name>D5GJI3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00009029001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430332">
        <property type="protein sequence ID" value="CAZ84676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840485.1">
        <property type="nucleotide sequence ID" value="XM_002840439.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJI3"/>
    <dbReference type="FunCoup" id="D5GJI3">
        <property type="interactions" value="87"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJI3"/>
    <dbReference type="GeneID" id="9182677"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009029001"/>
    <dbReference type="eggNOG" id="KOG4150">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000809_1_0_1"/>
    <dbReference type="InParanoid" id="D5GJI3"/>
    <dbReference type="OMA" id="GAVHLHQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17923">
        <property type="entry name" value="DEXHc_Hrq1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18797">
        <property type="entry name" value="SF2_C_Hrq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014939">
        <property type="entry name" value="CDT1_Gemini-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055227">
        <property type="entry name" value="HRQ1_WHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018973">
        <property type="entry name" value="MZB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47957">
        <property type="entry name" value="ATP-DEPENDENT HELICASE HRQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47957:SF3">
        <property type="entry name" value="ATP-DEPENDENT HELICASE HRQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09369">
        <property type="entry name" value="MZB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22982">
        <property type="entry name" value="WHD_HRQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01075">
        <property type="entry name" value="CDT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="4">
        <location>
            <begin position="292"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="5">
        <location>
            <begin position="516"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="198"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="206"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ84676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1071" mass="119433" checksum="DCD906E8B286822E" modified="2010-06-15" version="1">MDPEIKRKRTPKTKLSAAKKPRKGPTEEITPASIPIQWPPHFKNLQKLHEALNLTYTFCAQRKHVATTFDMLKSAVEGQTKMELTVEDVAQMKFLAPKCLRFSYVDEELLQLHVMGQAKDIYEDGKETLGMVLFLEFLDGEINQRSGKTPAVSQKAVLALIKRRNERFVAAVNKFLKGCAEKEVDAVGLLMDGCHEHVPRLPGHPQPSSQESLPTRGNSDIPKERKPIAEIIEEIKGLDLYREQIVPNGHRVIPPQEAAFGDLKFLLSQALVNALYTAHNVTRLYAHQVEAINNLCDGHSVIVSTSTSSGKSLIYQIPILHMMEQSARARTMAIFPTKALAQDQKRSLVEVLGYMTDVLGEVVVDTYDGDTELEDRDRVRQEARVIFTNPDMLHCSILPNKDRWKEFLQNLKYVIVDELHVYSGSFGSHTAFIMRRLRRVCASFGNTKVQFVSCSATVANPEAHMRTIFGVGSVKLTDVDGSPAGRKEFLCWNSPYKDPENLSLGRVDPVMETARLFTQLILRGIRAIAFCRARNACELLVKEVRSEFARLGRPEVAPRVMAYRGGYTPQDRRRIEKEMFEGHLLGIVATSALELGVDIGSLDAVIMVGFPHSISSLRQQSGRAGRRNKDSLSILVAGSTPRDQFHMDNPDEIFTKPNAELQVDLENPVIREGHLQCAAYEVPINLLEDHQYFGPNLEGFAESKLAQKNAGSYHPHESYLPYPSKHVSIREGPDLETHISVINTTNHTNQIIETLDPDHAAFTLYEGGIFLSQGKTYIVKNVNLSSRYAAVESLTVDWTTTPQDYTDIDPIETAVVHIIPESLSKAFYGTIRIHTVIYGFLKIDRAKRVLDAVEIDSPPITKLAKGMWLDVPVSALDILTLKNIDAAAAVHAAEHAVLNLFGNFVTSVSGAVKTECKSAVKSEEPEGKGKGKPTLKKKRRTRPARLIFYDAGATGMYTFRAFESVHVLLKQAVERMERCACLDGCFECIAPERYYSRLGLLIEYCIGIRSEHCQERNALISKPGAHVILRKILGLEVDLEKVPDGSVDGKGAVSRNKVILAVEIETGEASR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJK4</accession>
    <name>D5GJK4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00009052001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430332">
        <property type="protein sequence ID" value="CAZ84697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840506.1">
        <property type="nucleotide sequence ID" value="XM_002840460.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJK4"/>
    <dbReference type="FunCoup" id="D5GJK4">
        <property type="interactions" value="320"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJK4"/>
    <dbReference type="GeneID" id="9182697"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009052001"/>
    <dbReference type="eggNOG" id="KOG3038">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023535_1_0_1"/>
    <dbReference type="InParanoid" id="D5GJK4"/>
    <dbReference type="OMA" id="ASEYNHV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140671">
        <property type="term" value="C:ADA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046695">
        <property type="term" value="C:SLIK (SAGA-like) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140002">
        <property type="term" value="F:histone H3K4me3 reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072742">
        <property type="term" value="P:SAGA complex localization to transcription regulatory region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd20393">
        <property type="entry name" value="Tudor_SGF29_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20394">
        <property type="entry name" value="Tudor_SGF29_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000055">
        <property type="entry name" value="SAGA complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037802">
        <property type="entry name" value="SGF29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010750">
        <property type="entry name" value="SGF29_tudor-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047288">
        <property type="entry name" value="Tudor_SGF29_rpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047287">
        <property type="entry name" value="Tudor_SGF29_rpt2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21539">
        <property type="entry name" value="SAGA-ASSOCIATED FACTOR 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21539:SF0">
        <property type="entry name" value="SAGA-ASSOCIATED FACTOR 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07039">
        <property type="entry name" value="SGF29_Tudor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51518">
        <property type="entry name" value="SGF29_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="SGF29 C-terminal" evidence="7">
        <location>
            <begin position="144"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="104"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="27"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ84697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="279" mass="31230" checksum="8BADDDD87EC10F29" modified="2010-06-15" version="1">MAARNRPRAPLKEEADAHEERDLWTKIVRELQELQRMSERVQVLTTETQREEERHAEGDASLEDLKILKKLYTEHQELADKEQLTLQSVLEWNDLLIALRTATEGGDRGGEKKRKRKIDDAGAADSPSSRNTKVARSMSAAPDPHNGLQKDSEVAYRLPKQKNAEGMWIQCIIVGIAGDGNKRKYEVQDPEPDEAGGHGTTYKASANALILIPKDSAGLPPYPPGKQVLARYPETTTFYRAEVMGTKADGTCRLKFEGEEEVGKETEVERRLVLDVGNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJV6</accession>
    <name>D5GJV6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00009218001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Allantoate permease family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430334">
        <property type="protein sequence ID" value="CAZ84799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840608.1">
        <property type="nucleotide sequence ID" value="XM_002840562.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJV6"/>
    <dbReference type="FunCoup" id="D5GJV6">
        <property type="interactions" value="125"/>
    </dbReference>
    <dbReference type="GeneID" id="9186835"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009218001"/>
    <dbReference type="eggNOG" id="KOG2533">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_0_5_1"/>
    <dbReference type="InParanoid" id="D5GJV6"/>
    <dbReference type="OMA" id="CMFPLMC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000064">
        <property type="entry name" value="MFS allantoate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF1">
        <property type="entry name" value="ALLANTOATE PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="130"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="191"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="226"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="295"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="332"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="359"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="389"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="420"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="456"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="63"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ84799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="535" mass="59470" checksum="DBDFE6D33DE8F720" modified="2010-06-15" version="1">MAGKEISIPDSVRSISESNRFGSDIRLSSSGHDDALKALDGSEAVTLTPEKERKLLWKIDLHLIPIFSLLYGLQFLDKTTISYAAVMDIIQDTNLKGDNFNWLGILFPLFTAIFEVNWRHLMQKLPLAKFTAFNIVVWGGVLMLHAACSNFAGLTTVRFLLGIFEASISPAFLLFMSHWWRVREQAFRTSIWFCGNGVAQIVGSVCSYAMVLGIEKHGYTALGGWQLLFLSTGGLTVISGILFLFLVPDSPASAWFLNREERVMAIERLRGNNQGVGSKKFKKQQFFEAFRDPQTYYIAIWVITSTIPNGFLTNFKNLILKGFGLSAEKSLLYGAPSGAVQVVSCLIFGWLANKYQNRMAFATVAMFISLIGFALAVALPRSNLKGNLAAYYIISAAPAAFILLVSCISSNVAGYTKKTTVSAIVFIAYCAGNLIGPHTVDLKDGPMYVKAKTISLIAWCISGAILIALRFHYTSSNAARDKKMRELGDRYRHKVDQEFMDLTDMECAPPIPPAKIMEHKADNFSCRNLEFRYAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>D5GK89</accession>
    <name>D5GK89_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">histone deacetylase</fullName>
            <ecNumber evidence="3">3.5.1.98</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00009427001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N(6)-acetyl-L-lysyl-[histone] + H2O = L-lysyl-[histone] + acetate</text>
            <dbReference type="Rhea" id="RHEA:58196"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="3.5.1.98"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histone deacetylase family. HD type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.98" evidence="3"/>
    <dbReference type="EMBL" id="FN430337">
        <property type="protein sequence ID" value="CAZ84932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840741.1">
        <property type="nucleotide sequence ID" value="XM_002840695.1"/>
    </dbReference>
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</entry>
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        <lineage>
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            <taxon>Pezizomycetes</taxon>
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            <taxon>Tuber</taxon>
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        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
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                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
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                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ84933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="106" mass="11780" checksum="B38289317C84E859" modified="2010-06-15" version="1">MQSILYGGVKDQELTEWYFNTSLLFVGSDHLYWDGNKSRRKKYGNCKLTGGDVISTVMLNSQKEAASFMLSKITVKATENDGNNGHNDNKNGEEEEGEIIEGQIAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKE8</accession>
    <name>D5GKE8_TUBMM</name>
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        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00009505001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430338">
        <property type="protein sequence ID" value="CAZ84991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002840754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKE8"/>
    <dbReference type="GeneID" id="9182011"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009505001"/>
    <dbReference type="HOGENOM" id="CLU_446324_0_0_1"/>
    <dbReference type="InParanoid" id="D5GKE8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="283"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="354"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="448"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="286"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="305"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="319"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="364"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="448"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="466"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="477"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="612" mass="65907" checksum="95E888C3D1504A37" modified="2010-06-15" version="1">MPTDGNRAGQSRVRSTRYQKKSLKLRRTAAVPSQAISFPYRPHRRRDSPSNIRHPPRVYIPGSLMRPRQIREVIREDGARRIREIQTRVRSRSGPNRTNLPSGSGGPLASFYRISPPGAISDTSSQLCQLPSAPWPSNIPLSSRTCSEVTQYSSEPSDLAEHTCYQSSCTSSTGAPYHFPPLERIPGSAGTFDDFTSQVSAEPMMSSGFGCYSLEELESRLETITEDEFYKSTCSSLWSTEPFNHEQKRASIEMEHPGSLVNFCSSVTTGSAAPGTVHEALEQELSDSTTPHASSQADAEGAAVTSETSSALSSRPWTSKRSQSPRPSNQSPGGYGRTHILTPATITITTQTFERPASSAGHPHSSTATTRTSGSLSSASFKSPNESSVVNATPCVNQDAVTPFEASAPAVLSPIRTEIMPGTPDQLPPPAEQWEELGEQEVLGEQEVPGEQEVPGEQEVPSEPEAPEHKSQKSEDVQSNVSSSPSFPIHNDGCSICTFVPYQSSGADSVASQSGRSFSRNTPFPQYHNTSPREAMLMHSSAVRPNSRSLVPRVLAGEPIVDLQRELREAQLRQELERLGGAPNCVAGPAASLQATVESVAGDSDDTGGVDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKH4</accession>
    <name>D5GKH4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00009560001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the velvet family. VelB subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430339">
        <property type="protein sequence ID" value="CAZ85017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840826.1">
        <property type="nucleotide sequence ID" value="XM_002840780.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKH4"/>
    <dbReference type="STRING" id="656061.D5GKH4"/>
    <dbReference type="GeneID" id="9182036"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009560001"/>
    <dbReference type="eggNOG" id="ENOG502S1B4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022491_0_0_1"/>
    <dbReference type="InParanoid" id="D5GKH4"/>
    <dbReference type="OMA" id="YQDGRSW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030435">
        <property type="term" value="P:sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3960">
        <property type="entry name" value="Velvet domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021740">
        <property type="entry name" value="Velvet"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037525">
        <property type="entry name" value="Velvet_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038491">
        <property type="entry name" value="Velvet_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33572">
        <property type="entry name" value="SPORE DEVELOPMENT REGULATOR VOSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33572:SF3">
        <property type="entry name" value="VELVET COMPLEX SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11754">
        <property type="entry name" value="Velvet"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51821">
        <property type="entry name" value="VELVET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0749">Sporulation</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Velvet" evidence="8">
        <location>
            <begin position="59"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="23"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="142"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="24"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="142"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="153"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="175"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="200"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="219"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="237"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ85017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="366" mass="39625" checksum="856A8DDB3949C566" modified="2010-06-15" version="1">MYLAQPNHSNQPIHERHAIPPALPMARAPSSSSSSQSAPPANNGLIQSLQQPPSQSCRGDGNRIYSLEVKQQPIRARMCGFGDKDRRPITPPPCVRLVVKDATTGKEVDINEVDTSFYVLTVDLWSADASREVNLVRHSATSPSISAATSSSYPPPPPTPPLHPHPPAHVHHIPAHQQQLQHQGYPPPPPQQLQHQQAPGPYPGYHPVPSHAPPPPPQQQNYYQPPGAQGYYQGMHGPPPPQQQPPQQTPSGMFTRNLIGSLSASAFKLTDPDNKIGVWFILQDLSVRTEGAFRLKMNFVNVGRVDGGGLNTGSAPVLASAFSDVFHVFSAKKFPGVIESTGLSKCFATQGIKIPIRRDKGDGPEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKK4</accession>
    <name>D5GKK4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="12">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00009609001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430340">
        <property type="protein sequence ID" value="CAZ85047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840856.1">
        <property type="nucleotide sequence ID" value="XM_002840810.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKK4"/>
    <dbReference type="FunCoup" id="D5GKK4">
        <property type="interactions" value="618"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GKK4"/>
    <dbReference type="GeneID" id="9184709"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009609001"/>
    <dbReference type="eggNOG" id="KOG0767">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039456_1_0_1"/>
    <dbReference type="InParanoid" id="D5GKK4"/>
    <dbReference type="OMA" id="KMYIEEG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990547">
        <property type="term" value="P:mitochondrial phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000024">
        <property type="entry name" value="MIR1p Mitochondrial phosphate carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044677">
        <property type="entry name" value="SLC25A3/Pic2/Mir1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671:SF12">
        <property type="entry name" value="MITOCHONDRIAL PHOSPHATE CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 (MITOCHONDRIAL CARRIER PHOSPHATE CARRIER), MEMBER 3, LIKE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="21"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="119"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="217"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ85047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="313" mass="33084" checksum="E133FD129D1012E5" modified="2010-06-15" version="1">MSKAVEAVVSSKKPEQLSGLQLYSRFAFAGAVCCGVTHGALTPVDVVKTRIQLDPVTYNRGLVGGFKQVVQNEGAGALLTGFGPTCAGYFLQGAFKFGGYELFKQQSINILGYETASQNRTAVYLASSALAEFFADIALCPLEATRIRLVSQPDFASGLLSGFGKILKNEGVGAFYSGFGPILFKQVPYTMAKFVVYEKVAETVYSYWDKKSMSDGAQTTVNLGSGLIAGFAAAIVSQPADTMLSKINKTKGLPGEGTTTRLIKIAKELGFRGSYTGIGARLFMVGTLTAGQFAIYGDIKKALGAVGGVEISK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKQ1</accession>
    <name>D5GKQ1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00009698001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430340">
        <property type="protein sequence ID" value="CAZ85094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840903.1">
        <property type="nucleotide sequence ID" value="XM_002840857.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKQ1"/>
    <dbReference type="GeneID" id="9184756"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009698001"/>
    <dbReference type="HOGENOM" id="CLU_2833022_0_0_1"/>
    <dbReference type="InParanoid" id="D5GKQ1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="66" mass="7098" checksum="C842658D632DFF71" modified="2010-06-15" version="1">MSPLSAPEIQMAEEEGLTGCLTLPWWLSASLHAYEISAIDTSSLSTSVPSAGRGRTEFPASCRSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKU3</accession>
    <name>D5GKU3_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial</fullName>
            <ecNumber evidence="14">1.3.5.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">GSTUM_00009772001</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 14">
            <text>a quinone + succinate = fumarate + a quinol</text>
            <dbReference type="Rhea" id="RHEA:40523"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.3.5.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="13">Flavinylated by SdhE, about 5% flavinylation occurs in the absence of SdhE.</text>
    </comment>
    <comment type="pathway">
        <text evidence="14">Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (eukaryal route): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Mitochondrion inner membrane</location>
            <topology evidence="14">Peripheral membrane protein</topology>
            <orientation evidence="14">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.1" evidence="14"/>
    <dbReference type="EMBL" id="FN430341">
        <property type="protein sequence ID" value="CAZ85136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840945.1">
        <property type="nucleotide sequence ID" value="XM_002840899.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKU3"/>
    <dbReference type="FunCoup" id="D5GKU3">
        <property type="interactions" value="701"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GKU3"/>
    <dbReference type="GeneID" id="9183372"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009772001"/>
    <dbReference type="eggNOG" id="KOG2403">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014312_6_1_1"/>
    <dbReference type="InParanoid" id="D5GKU3"/>
    <dbReference type="OMA" id="PTGIWRM"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER01006"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045273">
        <property type="term" value="C:respiratory chain complex II (succinate dehydrogenase)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008177">
        <property type="term" value="F:succinate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006121">
        <property type="term" value="P:mitochondrial electron transport, succinate to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.700.10:FF:000001">
        <property type="entry name" value="Mitochondrial succinate dehydrogenase flavoprotein subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.80.40:FF:000002">
        <property type="entry name" value="Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.100:FF:000001">
        <property type="entry name" value="Succinate dehydrogenase flavoprotein subunit (SdhA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.100">
        <property type="entry name" value="Fumarate reductase/succinate dehydrogenase flavoprotein-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.80.40">
        <property type="entry name" value="succinate dehydrogenase protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.700.10">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-dep_OxRdtase_2_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037099">
        <property type="entry name" value="Fum_R/Succ_DH_flav-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015939">
        <property type="entry name" value="Fum_Rdtase/Succ_DH_flav-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030664">
        <property type="entry name" value="SdhA/FrdA/AprA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027477">
        <property type="entry name" value="Succ_DH/fumarate_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011281">
        <property type="entry name" value="Succ_DH_flav_su_fwd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014006">
        <property type="entry name" value="Succ_Dhase_FrdA_Gneg"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01816">
        <property type="entry name" value="sdhA_forward"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01812">
        <property type="entry name" value="sdhA_frdA_Gneg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE 2 FLAVOPROTEIN SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632:SF51">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE [UBIQUINONE] FLAVOPROTEIN SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02910">
        <property type="entry name" value="Succ_DH_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000171">
        <property type="entry name" value="SDHA_APRA_LASPO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46977">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56425">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6 13 14" id="KW-0274">FAD</keyword>
    <keyword evidence="5 13 14" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3 14" id="KW-0813">Transport</keyword>
    <keyword evidence="4 14" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="FAD-dependent oxidoreductase 2 FAD-binding" evidence="15">
        <location>
            <begin position="4"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="Fumarate reductase/succinate dehydrogenase flavoprotein-like C-terminal" evidence="16">
        <location>
            <begin position="400"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="227"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="339"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="344"/>
            <end position="345"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000171-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR611281-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR611281-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAZ85136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="535" mass="58882" checksum="6E76C864C87E45DD" modified="2010-06-15" version="1">MHPDDWRWHMYDTVKGSDWLGDQDAIHYMTREAPASVIELENYGCPFSRTEDGKIYQRAFGGQSQKYGKGGQAYRCCAAADRTGHALLHTLYGQSLRHNTKYFIEYFAMDLLMEDGECVGVIAYNQEDGTLHRFRAHKTVLATGGYGRTYFSCTSAHTCTGDGMAMVARAGLPNQDLEFVQFHPTGIYGAGCLITEGSRGEGGYLLNSEGERFMERYAPTAKDLASRDVVSRSMTLEIRNGRGVGPDKDHIYLQLSHLPAEVLHERLPGISETAAIFSGVDVTKSPIPVLPTVHYNMGGIPTRYTGEALTIDENGNDKVVPGLFAAGEAACVSVHGANRLGANSLLDLVVFGRAVAHTIRDTLTPGTPLKPVSADIGAASIANLDKIRNSDGSKSTAQIRLDMQKTMQTDVSVFRTQESLDQGVKKIQKVDASYHNLGIKDRSMIWNSDLVEALELGNLLTCATQTAHAAAARKESRGAHAREDYPERDDENWMKHTLSWQKEQHGKVDLKYRAVVGNTLDEKECAAVPPFKRVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>D5GL07</accession>
    <name>D5GL07_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Mitochondrial aspartate-glutamate transporter AGC1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Aspartate-glutamate carrier 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">GSTUM_00009867001</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Calcium-dependent mitochondrial aspartate and glutamate carrier. Transport of glutamate in mitochondria is required for mitochondrial transamination reactions and ornithine synthesis. Plays also a role in malate-aspartate NADH shuttle, which is critical for growth on acetate and fatty acids.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer (via N-terminus).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430342">
        <property type="protein sequence ID" value="CAZ85200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841009.1">
        <property type="nucleotide sequence ID" value="XM_002840963.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GL07"/>
    <dbReference type="STRING" id="656061.D5GL07"/>
    <dbReference type="GeneID" id="9185794"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009867001"/>
    <dbReference type="eggNOG" id="KOG0751">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014931_2_0_1"/>
    <dbReference type="InParanoid" id="D5GL07"/>
    <dbReference type="OMA" id="AFQNVMR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015183">
        <property type="term" value="F:L-aspartate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005313">
        <property type="term" value="F:L-glutamate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043490">
        <property type="term" value="P:malate-aspartate shuttle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000004">
        <property type="entry name" value="Calcium-binding mitochondrial carrier protein Aralar1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000552">
        <property type="entry name" value="Probable mitochondrial carrier protein ARALAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051028">
        <property type="entry name" value="Mito_Solute_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45678:SF9">
        <property type="entry name" value="CALCIUM-BINDING MITOCHONDRIAL CARRIER PROTEIN ARALAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45678">
        <property type="entry name" value="MITOCHONDRIAL 2-OXODICARBOXYLATE CARRIER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="EF-hand" evidence="17">
        <location>
            <begin position="99"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="17">
        <location>
            <begin position="171"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="15">
        <location>
            <begin position="346"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="15">
        <location>
            <begin position="444"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="15">
        <location>
            <begin position="542"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="704"/>
            <end position="725"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00059916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00073787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00082232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAZ85200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="725" mass="80739" checksum="73B7675E5B85C200" modified="2010-06-15" version="1">MAAVPPQPIQDSLLGVGQQPTLTSNIKENFEKFASIFEPTTGERLMTRENFIDAIAPPDQDFHKIKRDQYSILFDIADRRRQGVLNIQDWATFEDLLAKPDAEYQIAFRLFDVEGSGCVKYDDFVTFYTRSKGPSSLPFDWTSEWVALYLGGKNGRRNLSYPQFSQMLRGLHDERVRQAFCLYDKNKSGSIEPEDFERIILETSKHKLSDHLLNNLHTLSHISAGSKISYANVRAFQNVVREMNMIDLIIRNATAKSTDGKITRTDFMNEAAKITRFDLFSPMETDILFHFAGLNNTSGKLSITDFSRVLDSSWREPQFHPPPIVTGSLPTIPFILHKHGIFGQALDAAFNFALGSVAGAFGATIVYPIDLVKTRMQNQRSKVVGELMYKNSIDCAKKVIRNEGFRGLYSGLGPQLIGVAPEKAIKLTVNDLVRAKAKSKDGEISLPWELIAGGSAGACQVVFTNPLEIVKIRLQVQGEVAKNVEGVPRRSALWIVKNLGLVGLYKGASACLLRDVPFSAIYFPTYSHLKKDWFGESLTKKLGILQLLISGAMAGMPAAYLTTPCDVIKTRLQVEARKGQTHYRGLIHCASTIWREEGFKAFYKGGPARILRSSPQFGCTLAAYEVLQTLFHAQGNNTTESKGSAAGIQSTTMPSRVIAPLPYLRSRNALKLILVYPPPPILHLIRLSLLTLFAQDIDENIGRPKHPRTKEAFASIPGLARAQQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>D5GL81</accession>
    <name>D5GL81_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00010053001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430346">
        <property type="protein sequence ID" value="CAZ85274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841083.1">
        <property type="nucleotide sequence ID" value="XM_002841037.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GL81"/>
    <dbReference type="STRING" id="656061.D5GL81"/>
    <dbReference type="GeneID" id="9182068"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010053001"/>
    <dbReference type="eggNOG" id="ENOG502QUW1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007239_0_0_1"/>
    <dbReference type="InParanoid" id="D5GL81"/>
    <dbReference type="OMA" id="HWDVITR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042030">
        <property type="term" value="F:ATPase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028084">
        <property type="entry name" value="FNIP_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21634">
        <property type="entry name" value="RE13835P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21634:SF9">
        <property type="entry name" value="RE13835P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14636">
        <property type="entry name" value="FNIP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Folliculin-interacting protein N-terminal" evidence="2">
        <location>
            <begin position="153"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="183"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="237"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="592"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="674"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="782"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="927"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1061"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="49"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="198"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="607"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="941"/>
            <end position="951"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ85274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1134" mass="122082" checksum="2AFAC85951042D1A" modified="2010-06-15" version="1">MLGRLFSSANSAATSPNGTRPSTAAPESDDLHTRNLLYPDATCSSFHSSLSCSHTSSSSGGGFGENIDLDSDRDVRVIIAQDGTAAELKAVLYDSQPVPRNVGQPQPGFEDNGPLSPGLGGPHHRSRRRPMPPNHPFGASSYHPTQSASGVAPDHEMTVLTDCMFGSAPLAYKGPSTKVHILPTAEERKGRIRNSPVSSRRGSLRNLSSGPQQLETESHKDGRKEVLITRLFSVVMPPSPTFPNPPSGSKDHTSTPTSSPGSQHGFPFPKMANSAQPVSGPAAAAAKIVKPPKTYMYALGLIISLPPPAVYNRPSTTSSLSLSHKCCQLSTCTSFEHDHGHPDFLCSTPPPFDDGHFPQHSLNSLRSDAVVDDLQAPPSIDDRMDLITKHWDVINRALSDLQCVAQTQILNVLNTAGITSAQTVCQNGFKYRRRIELKQWALMSDDCVRGEVDRLRWRIVTGIKVPRVITGQGKWGVWRDEAHWANERFGGREMKFFFLTLLTAFLGHHTEWLDVLGPLPYRRKHQRQQQVAGNNEEFPIPTRTVILSSDKMASRRLIYLLSAFLPTKSFPQWDGLPPPSRTSSINYLSQSPPISGAMGNGMGGKKSSLRNRAKRKPSKLSMVTGDEEGVVVGWDIPAATSGESSTAPSVLQLPLANPARKAGSAGALTTAPSSVTAMPANAPGSPQKGDVRPSSSNSAASMNLMSTLKRTGTANTSMDSNSGWGSFLSFWSHSKTGSTAPSENSLQHDDGFDVIGRGGAISGDEREPVDVGELYLEDDDLQHHHHHKQHSAPPSLPAHHGFHNHHLQFSESQVRLSVDENDGVVDVDIPMPSLDFPTTGYTSPVSSPSSSGWAMSMPSLESMGNGFLGSGMSFSSGMVMPSWITDLEENAVNVAGWLDDEKFHPDFALQAVKPYPEVEEEIKRAMRAEATPRQPMGATPASESGGTSQESPNDRWIEVCSVLIADTRERMIKRLRLRRRSKKPVASPGTTSPLPSVLGAYRHLHIQQPSLVASGPYFGTAATAAAAQEGGEEEVIDEELVCDVDDVLATAVEKVIGISATPLPRPQTGGGGSRTTARDGGDELQKLNVQGCKCMVLGALENVVRAVAQEPGNVVENFLTEGVAKWLSDIQEAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLF1</accession>
    <name>D5GLF1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00010168001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430348">
        <property type="protein sequence ID" value="CAZ85344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841153.1">
        <property type="nucleotide sequence ID" value="XM_002841107.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLF1"/>
    <dbReference type="GeneID" id="9186088"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010168001"/>
    <dbReference type="eggNOG" id="ENOG502R59M">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005027_4_2_1"/>
    <dbReference type="InParanoid" id="D5GLF1"/>
    <dbReference type="OMA" id="LFAWRHF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006598">
        <property type="entry name" value="CAP10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051091">
        <property type="entry name" value="O-Glucosyltr/Glycosyltrsf_90"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12203:SF22">
        <property type="entry name" value="CAPSULE ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12203">
        <property type="entry name" value="KDEL LYS-ASP-GLU-LEU CONTAINING - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05686">
        <property type="entry name" value="Glyco_transf_90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00672">
        <property type="entry name" value="CAP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase CAP10" evidence="2">
        <location>
            <begin position="323"/>
            <end position="618"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ85344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="641" mass="73673" checksum="22743C1DA6531E61" modified="2010-06-15" version="1">MTLPPSSSRPRIFFILSISAFVIFLTNLTVITGTDYRGGIEILPIPQSLKRPGQRNKAPQLTIHEDKDDFSYSSEKADGHPISQLMEKAAADFKQYQDSRSKTFVQAIGKYRKKYGRHPPPGFDKAGLLYNRSEERNVYNVDDFDQIWDDLRPFWSIPPLELRKVVKGMLKEDPGNSVTGGNWRTDEFVKMLNVFVKELPDMQIAMNRLDQPRVIVEWEKLQEHLEIEEKSRNMDRPVTNGFSRNRELGGADESMSFPTAGWFSVPGIPYMDLASKACPPDSYARNNMTMDPAVAEAKYKFPNSGGLVSNFNISSDLCTVGPAIQDLHGMLFAPATIVATKELVPVFGECKVNVNNDILFPANMYYSDDPRYTYSPENDVPWDDKHTTVFWRGITSGGNQKLSTWRRLHRHRFVQLANGTDLHNTKTRLTLPTLDSTATPPAYIETKNWDPSPFSEKYTDIGFPELAWCVPDSKCGWLRTFLKEVNATTTLTDQFLNKYLPDIDGHSFSGRWHAWLKSRSLGIKATIFREWHDSRLFAWQHFAPMDNRFDDFYSIITYFIGLGNREEEEGVYIPTHDSTGKRLAGQGREWAAKVLRKEDIEVYMFRLMLEYARLLDDRREMIGYGGNGRRVKEEKLWVWPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLG7</accession>
    <name>D5GLG7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00010193001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430348">
        <property type="protein sequence ID" value="CAZ85360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841169.1">
        <property type="nucleotide sequence ID" value="XM_002841123.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLG7"/>
    <dbReference type="STRING" id="656061.D5GLG7"/>
    <dbReference type="GeneID" id="9186104"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010193001"/>
    <dbReference type="eggNOG" id="KOG1208">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_44_6_1"/>
    <dbReference type="InParanoid" id="D5GLG7"/>
    <dbReference type="OMA" id="LEMGTNC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320">
        <property type="entry name" value="RETINOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320:SF236">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ85360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="330" mass="36310" checksum="DD0ED87BF518F6F0" modified="2010-06-15" version="1">MESTTQALSEIFPPTPLFTEKDLPDLLSKVYIVTGGASGVGKELCKILYDKSATVYLAGRSQQNAENAISWIKSEVANTSGRLEFLNLDLNDLSTIKRSAQDFLAKENRLDVVWHNAGVMMPPSGSKTKQGYELQLGTNVIAPWLFQSFLTPLMQRTAALPDTLKDSVRVIWVSSGANNASPTPGGINWPDINFENVGWGIPRLFTLKFEIYGQSKAANIILGAEAVKRWSGSGILSVSLNPGNLRTALTRHVAGRFLTSIINLALYPPYYGALTELFAGLSPQVDESRNGSHVIPWGRFGNPRSDIVRGYGENGPKLWDWIESECREFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLP1</accession>
    <name>D5GLP1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00010294001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430350">
        <property type="protein sequence ID" value="CAZ85434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841243.1">
        <property type="nucleotide sequence ID" value="XM_002841197.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLP1"/>
    <dbReference type="GeneID" id="9182737"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010294001"/>
    <dbReference type="eggNOG" id="ENOG502SKWP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_279936_0_0_1"/>
    <dbReference type="InParanoid" id="D5GLP1"/>
    <dbReference type="OMA" id="NERAGWW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011044">
        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="177"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="250"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="351"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="691"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1086"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="351"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1086"/>
            <end position="1098"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1124" mass="125244" checksum="1BFC045C6D5137AE" modified="2010-06-15" version="1">MARGDMTGDNSVGCTALRWAGYEFEVRDVGRGSPPSCTDATSPETPFLDGKSWDGNSCALDSGVQRMRAEDSAWSGGVGLGLGLGIGAGEGPSVQPPISPSYPFSRMQLQDGLSPAGLKSVSEECGGWVEDGIMIPPLGENGDDIFGMDIDLEFALAGEDLGMVEMPALVMPEFIPDNGFSKQEGRPVPSPSPSPDFRVKRRHTDSDKTNKTTWHAEPLPTFPEGKYNFLGVGSSAHSASFLSPSFSSLPISSTGTTPTPPLSEGGGSLVRRTSSKRRRSEKRSSSRCVFCTIPDRPKDNQKIFLQNLPCEILLLIFEFSFISPTKFPKCEHRRNLPSGHISRRATAPIFTSSEPQPAQPLSSDQNGAAAAASTEVKIDESDPPRLPDHLRIYSAKTLLYISLTCTRFNEILMDSHVDATFWRSAARFCWNWLPENLCDVQGREESHQTSWRNLVGVFMRSENGLIKQKGGGKGGVESFGGNKGCVPATLWKDEQARRAVVGSEGMKKKKKLLLVCAQPGPDQLQVFPDKEGGYTLCLSLRAGKDSFVTLDEYGTFGKKPARRPGGLKWSTRGIGYFPPDIYEVEGDRYQLVKVRLRREASISTFSSVKAHPKPAVKEIVTWDLNCLPEYVADPKASVARCTSWEGTLVFNLFTHGGGEDQDGGIEALMDPPEDPRIFCVESLGYPGCHSTGKATATPMSEKKRGKMRASSVPLPGEQGTVYRWQQLFTYDRAGEYSPAVHLHYVICNLKLNSTKAVALIRWNIRTTKSNEELVDREFRIVDLKTGGVLRTLSFPNLYWDHRHHDMSVEYNLMRHQKMSRLYNHLGPGESMGAGNRCTRIHTDAFVLTETTLISGSHDYCNWVWDLSSPPPPPPNPPPVFTASHNEAGLHDPFTVLDDFYWSSAPEPGKELWNTNNERAGWWVRTPNQVLCFWHNISISPEGRFFAVCRPGKLFVWDLLNGGGNKVVGFTNIPPGAPASTRWLGRLSESASGLKHLLRHWFVWENVLPEQGLWLLYDDFTTVYLDRDDILSAAGLGKRAEWMFHREDFEMSEDEGEHGQCQHVHGRRRRSEFDDDCSEGDDCEDYYETSSDFDEEGEEHEAKKKRRTEEWEMLSFEAEEDLTFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>D5GM94</accession>
    <name>D5GM94_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00010594001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430353">
        <property type="protein sequence ID" value="CAZ85631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841440.1">
        <property type="nucleotide sequence ID" value="XM_002841394.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GM94"/>
    <dbReference type="GeneID" id="9187415"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010594001"/>
    <dbReference type="HOGENOM" id="CLU_2086552_0_0_1"/>
    <dbReference type="InParanoid" id="D5GM94"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="80"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="117" mass="13124" checksum="AC2C807780DDA100" modified="2010-06-15" version="1">MILDYRTDCCPIPSTTPFDRWQEPPSGSQRDQQILLQPVHLLQRSTNITPRINPTVDESALDIPSPIRRQKPCPKPLHATTTLPLTPTTINEPDQAPLLAAANPRRTDRVSAIVRDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMB2</accession>
    <name>D5GMB2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">rRNA biogenesis protein RRP5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Ribosomal RNA-processing protein 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00010634001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in the biogenesis of rRNA. Required for the formation of 18S and 5.8S rRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430354">
        <property type="protein sequence ID" value="CAZ85655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841464.1">
        <property type="nucleotide sequence ID" value="XM_002841418.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5GMB2">
        <property type="interactions" value="1059"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GMB2"/>
    <dbReference type="GeneID" id="9184304"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010634001"/>
    <dbReference type="eggNOG" id="KOG1070">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000845_0_0_1"/>
    <dbReference type="InParanoid" id="D5GMB2"/>
    <dbReference type="OMA" id="GQYLRAY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05702">
        <property type="entry name" value="S1_Rrp5_repeat_hs11_sc8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05703">
        <property type="entry name" value="S1_Rrp5_repeat_hs12_sc9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05693">
        <property type="entry name" value="S1_Rrp5_repeat_hs1_sc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05697">
        <property type="entry name" value="S1_Rrp5_repeat_hs5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05698">
        <property type="entry name" value="S1_Rrp5_repeat_hs6_sc5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05706">
        <property type="entry name" value="S1_Rrp5_repeat_sc10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05708">
        <property type="entry name" value="S1_Rrp5_repeat_sc12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000103">
        <property type="entry name" value="protein RRP5 homolog"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000155">
        <property type="entry name" value="rRNA biogenesis protein RRP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000159">
        <property type="entry name" value="rRNA biogenesis protein rrp5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000196">
        <property type="entry name" value="rRNA biogenesis protein RRP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000279">
        <property type="entry name" value="rRNA biogenesis protein rrp5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045209">
        <property type="entry name" value="Rrp5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057301">
        <property type="entry name" value="Rrp5_OB_4th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057302">
        <property type="entry name" value="Rrp5_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048058">
        <property type="entry name" value="Rrp5_S1_rpt_hs11_sc8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048059">
        <property type="entry name" value="Rrp5_S1_rpt_hs1_sc1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23270">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 11 PRE-RRNA PROCESSING PROTEIN RRP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23270:SF10">
        <property type="entry name" value="PROTEIN RRP5 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23240">
        <property type="entry name" value="HAT_PRP39_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24685">
        <property type="entry name" value="OB_RRP5_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23459">
        <property type="entry name" value="S1_RRP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="S1 motif" evidence="11">
        <location>
            <begin position="140"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="11">
        <location>
            <begin position="270"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="11">
        <location>
            <begin position="370"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="11">
        <location>
            <begin position="464"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="11">
        <location>
            <begin position="557"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="11">
        <location>
            <begin position="647"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="11">
        <location>
            <begin position="735"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="11">
        <location>
            <begin position="834"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="11">
        <location>
            <begin position="942"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="11">
        <location>
            <begin position="1038"/>
            <end position="1125"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="11">
        <location>
            <begin position="1140"/>
            <end position="1209"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="11">
        <location>
            <begin position="1234"/>
            <end position="1303"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="11">
        <location>
            <begin position="1324"/>
            <end position="1395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="92"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="184"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1437"/>
            <end position="1514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1765"/>
            <end position="1787"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="10"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="104"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="187"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="1437"/>
            <end position="1449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00055575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00073619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00076674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ85655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1787" mass="196371" checksum="97E2245489C2C12C" modified="2010-06-15" version="1">MGSEKKRKRPQDADPRRANKSETKKNPATANKRLKPEASTAASKFEPPKLSTFCSSKDEETSFPRGGGSVLTPLEFKQISNDAAKDVLFEAENAKAKSTKKKPMREELKKDKKSKRDESKKGEQKGIKAEGLSYKRLVPGTLVLGCISQINQTDLALSLPNNLTGFVPLTSISESLNKRVEALVRDSDDDEDEDVEEKIGTAKSESSEDDVDLKAMFQIGQYLRAYVVSSSELANSKSPSGSKKIKKRIELSLDPVMANNGLTTTELVVGCTVQASVTSVEDHGLVMNLGIGNDLKGFLSSKELGKGRSVTDAKEGQVMLCTTIGLSSNGKIVKLSGDLEQKPSKKGKYSGGKAAWWLSSAPTVDTFLPGTGVEVLITDIAKGSKAVGIVGKVMGLVDATMDFFHASGWREKDIESKFKVGEKIKARVIATYAEPHKLALSILPHVLSFTRPIENEPTTILPTSTIINSAKILNIEPKTGLSLDVGVPGVPGFVHISRVSSDSRIEVLSKDSGLHQTGSVHMARIIGYNSMDGLYLVSMEQKVLDQPFLRVEDIKIGEVVKGTINRVLDSGRVIVSLAEGITGIVDELHLSDIKLKHPEKKFREGIEVKARVLLTDPSKRKVMLTLKKAIVNSDAPIISSYEDTSSGTRSVGTLVKILPSGAVVKFFSDVCGFLPISEMSEAYIQDPHEHFTVGQSVNVHVLSVDPTNQKLRVSCRDPNLFGEVHKVALAKLPPGDVVSGTVVEKSADDLIVEINGLGAEGIRGVLVIGQLTDGSREKSLGVFKKLRAGQKLDGLLVLEKHEERRSITLSMKPSLVKAAKRGRMISKFEDVNEGERVKGWVRNTTLHGLFVGFAGGIVGVVYKRDLPAEVQSLPNFGFVKNQSITGRVVYIDPSERRFRLSLNSAKSEEKVSAVANTAGNSERLTVNPVDARFKLIDDYTPGKLTKAKVVSIQETQLNVKLADNVQGRIDVSLVFETWDAIKDKKSPLALFKKGDVLDAKVIGIHDARNHRFLAITHQKSNTKTPIFELSAKPSHIKEEGIEGCITKLEDITPNSTWVTFLNNISEECAWANIMPDIRGRIRILDLSDNVAQLKTLQKSFPVGCALKCQVLRVDSEHSKLDLSARSPSSSALTFDKLAKGMVVPGRVTKVTDRQVLVQISESVSGPISLTDLADDFSQANTNGYSKNEVIRVCIIDIDSPNKRVTLSARPSRVMISDLPVKDPEIQAIVNVKVGDIRRGFVKQVSPKGLFVTIGGNVTAWTKISDLSDGFLKEWEGLFTVGQLVKGKIMAVDSTLGHVQMSLRESDISGKEKPKPADYSSLKEGQIVKGKVKNIAEYGVFISVDGSQNISGLCHKSQIADSAIDDISKLYATGDPVKAKIISIDLEKKRISFGLKSSYFKDEVSEDESEDGSESEGGADLSAVKVCLGLFWIDADGESDEREQEQEEPSVEMNDAPTSEGLSTGGFDWSASILDKRGPDTDSEGDSNSEEEKHKKKKRKKAQIKEDLTGNLATREPQSVADFERLLLGDPNDSKLWIMYMSFELQLSEVEKARGIAERAIKTIALREESEKQNVWIAMLNMESMYGTDETLEEAFKRACQYNEAQGMHEKLASIYIQTSKTEKADDLFKVMIKKFSQDPKIWVNYADFLLSSKQSCDAARALLQRAMQALPQSQHKDLISKFAKLEFRSGDPERGRTLFENLLATFKKKSDLYNMFLDMEIKYGSEEEDGKEGVRALFKRALAEKTSTRQAKALFKKWLEFEKSKGDKKSTETVTRKAKEYVEAKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>D5GML3</accession>
    <name>D5GML3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="12">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00010788001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in pre-mRNA splicing. Facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome. Binds to RNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLT11 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430357">
        <property type="protein sequence ID" value="CAZ85756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841565.1">
        <property type="nucleotide sequence ID" value="XM_002841519.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GML3"/>
    <dbReference type="STRING" id="656061.D5GML3"/>
    <dbReference type="GeneID" id="9182182"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010788001"/>
    <dbReference type="eggNOG" id="KOG0153">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027112_1_0_1"/>
    <dbReference type="InParanoid" id="D5GML3"/>
    <dbReference type="OMA" id="CPLRVQW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071006">
        <property type="term" value="C:U2-type catalytic step 1 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036002">
        <property type="term" value="F:pre-mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017070">
        <property type="term" value="F:U6 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12265">
        <property type="entry name" value="RRM_SLT11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000396">
        <property type="entry name" value="Putative Pre-mRNA-splicing factor slt11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039171">
        <property type="entry name" value="Cwc2/Slt11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034356">
        <property type="entry name" value="Slt11_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048995">
        <property type="entry name" value="STL11/RBM22-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR RBM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089:SF6">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR RBM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21369">
        <property type="entry name" value="STL11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="221"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="156"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="315"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="331"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ85756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="364" mass="39143" checksum="C85F195AA1416675" modified="2010-06-15" version="1">MPPAGPKSDINRSGSEVTDFPAVCNDCLSATDNPFIQMIKEDHGMECKICTRPFTVFRWAADRTSRFKKTNICLTCARLKNCCQCCMLDLSFGLPIAVRDAALKLVAQGPSSGINKEYYAQNSEGTLVDAQVPEEYEKTDDRARELLKRLAGSEPYYKRRRGGGGNGNDGEGSRRVGPVRNRGGGGGRGRGGKGRGARFPGVSQIPPGPMDWAPPEDTTITSLFLTGVEDDLAEHHLRTFFAPHGPIKSVVCVHRSRCAFINFTTRAAAESATATCQGKAVVAGCPLRVQWGRTRPLGNMDRGQSAAVVRDVNPAAAGGGKEEEVVDAGDMEGREDGGRDVREEIVLARPPGEDDVVYPSQVGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMQ9</accession>
    <name>D5GMQ9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00010890001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430359">
        <property type="protein sequence ID" value="CAZ85802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841611.1">
        <property type="nucleotide sequence ID" value="XM_002841565.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMQ9"/>
    <dbReference type="GeneID" id="9185138"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010890001"/>
    <dbReference type="HOGENOM" id="CLU_3070389_0_0_1"/>
    <dbReference type="InParanoid" id="D5GMQ9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ85802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="53" mass="5981" checksum="976450628BD5241B" modified="2010-06-15" version="1">MIPCSLNTLLSPPYLSRDIINSASQSSTKIGQCTHCHPVRHILVLYCSKLRQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>D5GN63</accession>
    <name>D5GN63_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00011105001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the putative lipase ROG1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430362">
        <property type="protein sequence ID" value="CAZ85956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841765.1">
        <property type="nucleotide sequence ID" value="XM_002841719.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GN63"/>
    <dbReference type="GeneID" id="9185882"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011105001"/>
    <dbReference type="eggNOG" id="KOG4372">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027968_1_1_1"/>
    <dbReference type="InParanoid" id="D5GN63"/>
    <dbReference type="OMA" id="FCTPHVG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007751">
        <property type="entry name" value="DUF676_lipase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044294">
        <property type="entry name" value="Lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482:SF65">
        <property type="entry name" value="ESTERASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G12320)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482">
        <property type="entry name" value="LIPASE ROG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05057">
        <property type="entry name" value="DUF676"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="270"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="DUF676" evidence="5">
        <location>
            <begin position="7"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="384"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ85956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="484" mass="54209" checksum="CD7908274773F3C9" modified="2010-06-15" version="1">MGIPSGKGDHLCVLIHGLWGNPGHLEYLTTAIRQRYDDSKLQILVAKRNSGTFTYDGIELGGERVTREIEDAIEEYARNGVEIRKISIVGYSLGGLVARYAVGLLYSKGYFDRIRPVNFCTFVAPHLGVRTPLLGWHNHIWNVIGARLLSASGRQLFAIDKFRNTGRPLLSILADKDSVFFKGLERFQNRVLYANVVNDRATCYYTAGISRFDPYAALPDISLNYVPGYSPIVLDPDSPISPATREEDEKLPVAHRISRTTHVAMRLPMVLIYTIVIPIGMLVFLLNSLIQTVFSRRRIRLHSSDQLGYHRLPLLVEEMQEAAEGLIEDIHHEMIPQHLTDISEESVGEKLVNAITPRKTVPSQEVAEDSSVLITLVDGTSRPVENASGDSGVPPPKSAPAPQTVTEKLPPLAQGKLEFPTLALSHQQFEMIANIDKLGFRKYPVFIHNDRHSHAAIIVRKPWAKRWDEGRVVVAHWLDKAFIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNB1</accession>
    <name>D5GNB1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00011205001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430363">
        <property type="protein sequence ID" value="CAZ86004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841813.1">
        <property type="nucleotide sequence ID" value="XM_002841767.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNB1"/>
    <dbReference type="FunCoup" id="D5GNB1">
        <property type="interactions" value="423"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GNB1"/>
    <dbReference type="GeneID" id="9182204"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011205001"/>
    <dbReference type="eggNOG" id="ENOG502QPWC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009666_1_1_1"/>
    <dbReference type="InParanoid" id="D5GNB1"/>
    <dbReference type="OMA" id="HHIAMMA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030907">
        <property type="term" value="C:MBF transcription complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033309">
        <property type="term" value="C:SBF transcription complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048315">
        <property type="term" value="P:conidium formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030435">
        <property type="term" value="P:sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.260.10:FF:000001">
        <property type="entry name" value="APSES transcription factor (MbpA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.260.10">
        <property type="entry name" value="Transcription regulator HTH, APSES-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036887">
        <property type="entry name" value="HTH_APSES_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018004">
        <property type="entry name" value="KilA/APSES_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051642">
        <property type="entry name" value="SWI6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003163">
        <property type="entry name" value="Tscrpt_reg_HTH_APSES-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43828">
        <property type="entry name" value="ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43828:SF3">
        <property type="entry name" value="CHROMO DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04383">
        <property type="entry name" value="KilA-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01252">
        <property type="entry name" value="KilA-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54616">
        <property type="entry name" value="DNA-binding domain of Mlu1-box binding protein MBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51299">
        <property type="entry name" value="HTH_APSES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0183">Conidiation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0749">Sporulation</keyword>
    <feature type="domain" description="HTH APSES-type" evidence="8">
        <location>
            <begin position="13"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="322"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="458"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="168"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="607"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="561"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="188"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ86004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="748" mass="82015" checksum="E8B7B3BFEAFACFCA" modified="2010-06-15" version="1">MAPMDSADSKPTIFTATYSGVSVFEMPVNNVAVMRRRHDSWLNATQILKVAGVEKGKRTKVLEKEILTGPHEKVQGGYGKYQGTWIDFERGREFCRAYGVEDLLAPLLDLDVQAASGPDSTPTKEQAMAARRKRMYNSSLNQANGNSGPLFPPISATVTNAFNALSKSSARMDSPGPRGGYSQPAPPSSQITTRPQESFSQESWTINLPSETSFSQSQTQPDSAYASQSFTTVDSQIESQEPPRKRVRASTPPHEALAPFFPTHLDTPSKPVQPLAAEQVPDSERARQILMNLFINPETDNTVLSHLDSLTPDQIDIPLDSTGSAAIHWSASLARVPLIRALITKGASISRVNHAGETALVRGCFVTNNFDQSSFPQLLNLLHPTIPIADNNGRTILHHIAVKSGMKGRSADSRYYLHCLLEFVAKHGASATSQRTSGPKVMSLARFIGEIVNAQDKSGDTALNIAARIGNKSIIQQLLEVGADSSIPNRAGLRPIDFGVGGEPAPVPSRREQGWILPPAVVQKRQDIMKAIKGLIAGTEKDFQGEIEAKQEQVTGTLNRLREVTLKLGKEKEKMNQLRQRSRQHSELKQHCQNLRRAIEEENVRLQGKGGNTNGDVPSLKGERLDPDRPFKIKMELLAQSGDSREIGERQKQYLKSLPSTAVLKARVRAYTTNGENLREVAGKLHDKSTDLEDKFRRVVALCTGLEEYKIDSLLEGLVQAVESDPGEVDTARVSSFLRKVDESTGAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNJ3</accession>
    <name>D5GNJ3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00011313001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SF3B4 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430365">
        <property type="protein sequence ID" value="CAZ86086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841895.1">
        <property type="nucleotide sequence ID" value="XM_002841849.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNJ3"/>
    <dbReference type="FunCoup" id="D5GNJ3">
        <property type="interactions" value="291"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GNJ3"/>
    <dbReference type="GeneID" id="9186162"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011313001"/>
    <dbReference type="eggNOG" id="KOG0131">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_21_1_1"/>
    <dbReference type="InParanoid" id="D5GNJ3"/>
    <dbReference type="OMA" id="AMVYEIM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048026">
        <property type="term" value="P:positive regulation of mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12334">
        <property type="entry name" value="RRM1_SF3B4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12335">
        <property type="entry name" value="RRM2_SF3B4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000895">
        <property type="entry name" value="Hsh49p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000121">
        <property type="entry name" value="Splicing factor 3b subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034158">
        <property type="entry name" value="SF3B4_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034159">
        <property type="entry name" value="SF3B4_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052084">
        <property type="entry name" value="SF3B4_spliceosome_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48030">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48030:SF3">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="13"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="101"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="214"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="224"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="269"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="293"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="348"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ86086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="392" mass="41831" checksum="72FB6A92B328AF88" modified="2010-06-15" version="1">MSGARHWEQDKEATVYIGNLDERVSDALVWELMLQAGRIVNVHLPKDRVTQTHQGFGFVEFISEEDAEYAARIMNQVRLYGKPIRVNKASADKQKTVEVGAELFVGNLDPMVDEKTLFDTFTTFGNLVSAPKIARDDQGISKGFGFISYDSFEASDKAIESMHNQFLMNKEINVQYAYKKDGKGERHGDQAERLLAAQAKKHNVQITPQQLPAQLFAPTGPTSGVPPIPVGAGPPAPSAPGNFQRGPPARPQNGMTNPHDQGFQGGFHAPPPGPPQGLPPAGRGGAPQHHAPPHQPQLAPPPQGLPARPPPPVGFQPHQQHAYGRPPPPVGFAQPMHPGFSQPPPLPAGFGSPQQQAPQGFPGQAPGAPPGATGFAPPGPPPPGYGGYGRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNK3</accession>
    <name>D5GNK3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="11">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00011325001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430365">
        <property type="protein sequence ID" value="CAZ86096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841905.1">
        <property type="nucleotide sequence ID" value="XM_002841859.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNK3"/>
    <dbReference type="FunCoup" id="D5GNK3">
        <property type="interactions" value="112"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GNK3"/>
    <dbReference type="GeneID" id="9186172"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011325001"/>
    <dbReference type="eggNOG" id="KOG0314">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019105_1_0_1"/>
    <dbReference type="InParanoid" id="D5GNK3"/>
    <dbReference type="OMA" id="NVPDHEP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16620">
        <property type="entry name" value="vRING-HC-C4C4_RBBP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.60.10:FF:000005">
        <property type="entry name" value="E3 ubiquitin-protein ligase RBBP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014891">
        <property type="entry name" value="DWNN_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033489">
        <property type="entry name" value="RBBP6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025829">
        <property type="entry name" value="Zn_knuckle_CX2CX3GHX4C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15439:SF0">
        <property type="entry name" value="CELL DIVISION CYCLE AND APOPTOSIS REGULATOR PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15439">
        <property type="entry name" value="RETINOBLASTOMA-BINDING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08783">
        <property type="entry name" value="DWNN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13696">
        <property type="entry name" value="zf-CCHC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01180">
        <property type="entry name" value="DWNN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51282">
        <property type="entry name" value="DWNN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DWNN" evidence="10">
        <location>
            <begin position="5"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="9">
        <location>
            <begin position="191"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="8">
        <location>
            <begin position="301"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="97"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="231"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="369"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="621"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="121"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="369"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="433"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="473"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="666"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="678"/>
            <end position="687"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ86096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="687" mass="74421" checksum="77B8C07C691E0D24" modified="2010-06-15" version="1">MSSSVFFRFKSHKESTRVTFDGTGISVFELKRQIIVLNRLGDGTDFDFSIYNADTNEEYDDDTTIIPRSTSIIAARLPPSKPGRGTAQRYVSGKAPVNALPNAGRREKNFGNGGSVVKKNGSPQPTSSAPVLTGGESEEEKIAAMFQASSEQWNKTQEQMANATPIHRGSQGFKKNNAPAPGHPPPNGYICYRCGEKGHWIQQCPTNADPTFDGRPRVKRTTGIPRSFLKTVEKPLPPSGDDDNTTAHSNPTSVMVNADGEYVVAQPDQASWESYQKKAATNSKVAAPVGSKELQDKGIECLICHKLLRDASKTPCCGKVYCEDCIHTTLLETDFVCPNCDAKEILLDAIVPDEDIRKKVEEYLKERDAKEKDLKEREKSKSPSVSSSAGKAKEPSASQRGDGTPSAAPSHGRAPPSVHGSMAGNFGPNPRKRQADEEFEPRIPRGPAAMRNNNNPQPQQPPPHPMQPLQQQSGGYNNNGRMFNPQQVFHHQFPNQNTHQPTPLPPYQFQAFNQYPSGMNGMDNMNAMNGNYYPQNGHYVPNGMVGMGGMGMNGMMGGGMGGGMGGGMGGGMGGGMGGGMGGMGPHNPYHMGMNGGGGGGAAMNVSNGHGGNPMINTSFAQQFQHQHQHPPGDPRAKFGYFPNQQKTVFSEPFPSEEDSPYMRKPVNPHRHSRPKRIRPSDFKALGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNR4</accession>
    <name>D5GNR4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00011440001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430367">
        <property type="protein sequence ID" value="CAZ86161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841970.1">
        <property type="nucleotide sequence ID" value="XM_002841924.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNR4"/>
    <dbReference type="GeneID" id="9182796"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011440001"/>
    <dbReference type="HOGENOM" id="CLU_2591522_0_0_1"/>
    <dbReference type="InParanoid" id="D5GNR4"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ86161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="80" mass="8658" checksum="64C20377A41DF288" modified="2010-06-15" version="1">MGSKEGRVIHPNDGSAEKYWTIKSSLGPRTVTRQILPMQIDGAGKGKRFFRKKTKDGASERVGGGFTGTGSLVSYVSVKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNV5</accession>
    <name>D5GNV5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 16">Diacylglycerol O-acyltransferase</fullName>
            <ecNumber evidence="5 16">2.3.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">GSTUM_00011528001</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 16">
            <text>an acyl-CoA + a 1,2-diacyl-sn-glycerol = a triacyl-sn-glycerol + CoA</text>
            <dbReference type="Rhea" id="RHEA:10868"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:64615"/>
            <dbReference type="EC" id="2.3.1.20"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 16">Glycerolipid metabolism; triacylglycerol biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Endoplasmic reticulum membrane</location>
            <topology evidence="1 16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the diacylglycerol acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.20" evidence="5 16"/>
    <dbReference type="EMBL" id="FN430370">
        <property type="protein sequence ID" value="CAZ86198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842007.1">
        <property type="nucleotide sequence ID" value="XM_002841961.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNV5"/>
    <dbReference type="FunCoup" id="D5GNV5">
        <property type="interactions" value="205"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GNV5"/>
    <dbReference type="GeneID" id="9182832"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011528001"/>
    <dbReference type="eggNOG" id="KOG0831">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023995_3_1_1"/>
    <dbReference type="InParanoid" id="D5GNV5"/>
    <dbReference type="OMA" id="FWFTCAN"/>
    <dbReference type="UniPathway" id="UPA00282"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004144">
        <property type="term" value="F:diacylglycerol O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006672">
        <property type="term" value="P:ceramide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006071">
        <property type="term" value="P:glycerol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035356">
        <property type="term" value="P:intracellular triglyceride homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019915">
        <property type="term" value="P:lipid storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07987">
        <property type="entry name" value="LPLAT_MGAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007130">
        <property type="entry name" value="DAGAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12317:SF0">
        <property type="entry name" value="ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12317">
        <property type="entry name" value="DIACYLGLYCEROL O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03982">
        <property type="entry name" value="DAGAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 16" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="10 16" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0319">Glycerol metabolism</keyword>
    <keyword evidence="6 16" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="12 16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="159"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="61"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="73"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="84"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU367023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAZ86198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="500" mass="56049" checksum="2D7DEB2584A0D6AF" modified="2010-06-15" version="1">MQPHTEEDILSSSSEDGFDLLAPAPVPKELPKSYAAAAPEPVLPSGDWNINDVSGDGKVGGKFYASEIAKPPHGGRRGNGVKRYHGDDKEYKEPMSHRKTGGSSTSPDGYETKDKTPRRGKGRLVSGDYPVSSNLTRVGSIRFAPLNIPLKRRLQTTAVLWHTFAIAIFVSLFWFLLAIPLTWPLLIPYLIYVLYSNSHRDGSSPVIRSSLFRSLPMWRLLTGYFPLNLHRSSPLPPNRNYIFTYHPHGIISHGAFGHFCTEGTTGFEKLFPGIKNTLLTLESNFRIPFYREYLLKLGLASVSRRSCEALLRGEGKARQSWLAKKFFKSPPPQPAPGGRAITIVVGGARESLEAIPGTMKLVLKRRRGFLKIAIRENAGVVPVLSFGENDLYDQVVPSSNSLIRKLQMLVKKTAGFTVPLFHARGVFNYDVGFLPYRREVNTVIGHPIFPKQQSDSPRDPEVDELQERYIAELQRLWDEWKDVFAKDRQPGEEGELVILE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GP25</accession>
    <name>D5GP25_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00011634001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430371">
        <property type="protein sequence ID" value="CAZ86268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842077.1">
        <property type="nucleotide sequence ID" value="XM_002842031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GP25"/>
    <dbReference type="GeneID" id="9183561"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011634001"/>
    <dbReference type="HOGENOM" id="CLU_1950384_0_0_1"/>
    <dbReference type="InParanoid" id="D5GP25"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ86268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="129" mass="13797" checksum="243ECF23A92E4FBE" modified="2010-06-15" version="1">MDGIRCSAGIGSKLLRVWHTSTGDKANGGVNYRSSVLFFSPHTFAPSLVTPIMFTSTDTGTVDGQVVQEVCHDTGYSGMLCLQSALLKGVGMIVRHAAGIGGCATNGTVRYRYRYSSGYEDFTSEGETR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPH9</accession>
    <name>D5GPH9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00011851001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transient receptor potential (TRP) ion channel family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430375">
        <property type="protein sequence ID" value="CAZ86422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842231.1">
        <property type="nucleotide sequence ID" value="XM_002842185.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPH9"/>
    <dbReference type="FunCoup" id="D5GPH9">
        <property type="interactions" value="45"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GPH9"/>
    <dbReference type="GeneID" id="9186662"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011851001"/>
    <dbReference type="eggNOG" id="ENOG502QSVZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010226_1_0_1"/>
    <dbReference type="InParanoid" id="D5GPH9"/>
    <dbReference type="OMA" id="EFRNGWR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009272">
        <property type="term" value="P:fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010308">
        <property type="entry name" value="TRP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040241">
        <property type="entry name" value="TRP_Flc/Pkd2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032800">
        <property type="entry name" value="TRP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31145:SF2">
        <property type="entry name" value="FLAVIN CARRIER PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31145">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_7G01610)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06011">
        <property type="entry name" value="TRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14558">
        <property type="entry name" value="TRP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01320">
        <property type="entry name" value="TRP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="220"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="274"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="300"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="363"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="389"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="420"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="447"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="domain" description="ML-like" evidence="9">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="522"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="537"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="591"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="603"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="637"/>
            <end position="650"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ86422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="661" mass="71948" checksum="226DDAB704BEBCF5" modified="2010-06-15" version="1">MGEGFQGMCPMQQGLIQLESHTPIEADVARQIPAIAYQVPDIDGKAKIMVNRTDTKQMIACVEAPLSNGKTVDTKGVGWATAIIAGMGLIASAVTSGLGHSNTAAHVAANALSLFGYFQAQAMIGMTGVTLPPIVAAWTQNFDWSMGIIKVGFMQEIFHWYIQATGGKPSNIFGSLSEVSVQIMKRSLPEQTSSLISRAAAYVWGIKRVSFKAKIEATNFFMTGLAFLVAFLFFVALGVCAFKAICEFASKMQWIKGTKFQDFRNGWMTVLKGIMYRLVLIGYPQMAILCLWELTVRDSGGAVTLAIFFFFSMTGILAWASFKVIRIAQRSVTLHKNPAYILYSDPAALNRWGFLYVQFRATAYYYVVPVLLYTLVKAMFIAFAQNKGLVQAIALLLIEVAYLIAVSIMKPWMDKRTNIFNISICAINFLNTVFLLVFTDVFGGPGIVVGVMGVIFFILNAAFALVLLILVLISSIYALISKNPDVRYQPMRDDRASFIKSNTQMAASTELDALGVTARGDKGGYKTRDLEDDDDSYTTNSMNKEASQIPLPPSTAGSHAPSHRHMDTPPSPLNPAVPMFPTAGHDGSRGPYNSQMAQHSNQLPLLTTSGTPRSPSRSGVPSPSSPARSPGGYDYSQPVQRSNTGGSMNWHTGAGYDGRGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPN9</accession>
    <name>D5GPN9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="19">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">GSTUM_00011952001</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RBT5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="FN430377">
        <property type="protein sequence ID" value="CAZ86482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842291.1">
        <property type="nucleotide sequence ID" value="XM_002842245.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPN9"/>
    <dbReference type="STRING" id="656061.D5GPN9"/>
    <dbReference type="GeneID" id="9184305"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011952001"/>
    <dbReference type="HOGENOM" id="CLU_1620268_0_0_1"/>
    <dbReference type="InParanoid" id="D5GPN9"/>
    <dbReference type="OMA" id="VINCLAT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051735">
        <property type="entry name" value="CFEM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008427">
        <property type="entry name" value="Extracellular_membr_CFEM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37928">
        <property type="entry name" value="CFEM DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_6G14090)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37928:SF2">
        <property type="entry name" value="GPI ANCHORED CFEM DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_6G10580)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05730">
        <property type="entry name" value="CFEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00747">
        <property type="entry name" value="CFEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52012">
        <property type="entry name" value="CFEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12 15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="6" id="KW-0349">Heme</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="14" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="9 17" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072893" evidence="17">
        <location>
            <begin position="19"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="CFEM" evidence="18">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="101"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="47"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS52012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAZ86482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="164" mass="15577" checksum="C28ADD75CC00075E" modified="2010-06-15" version="1">MKTSIIASILGAALLVAAQTEVPSLSDLPSCALPCAGDAVSGTGTGCQAGNIPCACRNEKFISDVSNCLRAACTEPQDIQKAISVAQGLCGTNLPSGAVSSIQANPTGNTTSSGSGNNSTKTTSGGGPGGSSGSATSTSETGGAAGLVPGGFAIAAIFAGLLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPU5</accession>
    <name>D5GPU5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial</fullName>
            <ecNumber evidence="15">7.1.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">GSTUM_00012037001</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="function">
        <text evidence="15">Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Essential for the catalytic activity and assembly of complex I.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 15">
            <text>a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29091"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="7.1.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 15">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 15">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="14">Complex I is composed of about 40 different subunits. This is a component of the flavoprotein-sulfur (FP) fragment of the enzyme.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Mitochondrion inner membrane</location>
            <topology evidence="15">Peripheral membrane protein</topology>
            <orientation evidence="15">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the complex I 51 kDa subunit family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.2" evidence="15"/>
    <dbReference type="EMBL" id="FN430378">
        <property type="protein sequence ID" value="CAZ86538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842347.1">
        <property type="nucleotide sequence ID" value="XM_002842301.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPU5"/>
    <dbReference type="STRING" id="656061.D5GPU5"/>
    <dbReference type="GeneID" id="9182282"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012037001"/>
    <dbReference type="eggNOG" id="KOG2658">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014881_1_0_1"/>
    <dbReference type="InParanoid" id="D5GPU5"/>
    <dbReference type="OMA" id="QGDGKPH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.230:FF:000001">
        <property type="entry name" value="Mitochondrial NADH dehydrogenase flavoprotein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.600:FF:000001">
        <property type="entry name" value="NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11540:FF:000001">
        <property type="entry name" value="NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.600">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11540">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase 51kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.230">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase 51kDa subunit, iron-sulphur binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050837">
        <property type="entry name" value="ComplexI_51kDa_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001949">
        <property type="entry name" value="NADH-UbQ_OxRdtase_51kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011537">
        <property type="entry name" value="NADH-UbQ_OxRdtase_suF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011538">
        <property type="entry name" value="Nuo51_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037225">
        <property type="entry name" value="Nuo51_FMN-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019575">
        <property type="entry name" value="Nuop51_4Fe4S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037207">
        <property type="entry name" value="Nuop51_4Fe4S-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054765">
        <property type="entry name" value="SLBB_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01959">
        <property type="entry name" value="nuoF_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010120">
        <property type="entry name" value="PRK13596.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11780:SF10">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] FLAVOPROTEIN 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11780">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE FLAVOPROTEIN 1 NDUFV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01512">
        <property type="entry name" value="Complex1_51K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10589">
        <property type="entry name" value="NADH_4Fe-4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22461">
        <property type="entry name" value="SLBB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00928">
        <property type="entry name" value="NADH_4Fe-4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142019">
        <property type="entry name" value="Nqo1 FMN-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142984">
        <property type="entry name" value="Nqo1 middle domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140490">
        <property type="entry name" value="Nqo1C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00644">
        <property type="entry name" value="COMPLEX1_51K_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00645">
        <property type="entry name" value="COMPLEX1_51K_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 15" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="15" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6 15" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7 15" id="KW-0288">FMN</keyword>
    <keyword evidence="10 15" id="KW-0408">Iron</keyword>
    <keyword evidence="11 15" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="15" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12 15" id="KW-0520">NAD</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="15" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="15" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NADH-ubiquinone oxidoreductase 51kDa subunit iron-sulphur binding" evidence="16">
        <location>
            <begin position="382"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00063883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU364066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAZ86538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="499" mass="54557" checksum="6654F886EEDF570C" modified="2010-06-15" version="1">MFLARNPQCGVIALASSHLARSSAIAVYRQQGSRNLATVSDTPTRSYGGLKDQDRIFQNLYGHHGTDLKSAMKYGDWYKTKEILLKGHDWIIGEIKTSGLRGRGGAGFPSGMKWSFMTPPGWNTGTTPRYLVVNADEGEPGTCKDREIMRKDPHKLVEGCLVAGRAMEATAAYIYIRGEFYHEATVLQRAIQEAYSAGLIGKNACGSGYDFDVFVHRGMGAYVCGEETSLIESLEGKPGKPRLKPPFPASVGLFGCPSTVANVETVSVAPTICRRGGSWFASFGRKRNEGTKLFCISGHVNNPCTVEEEMSIPLKELIERHCGGVRGGWDNLQAIIPGGSSTPILPRKICDDVLMDFDALKDVQSGLGTAAVIVMDKSTDVVRAISRLSKFYKHESCGQCTPCREGTRWTAQIMERFEKGQGREREIDMLQELTKQVEGHTICALGEAFAWPLQGLIRHFRPELEARMQAHAQASGGEALAGGWEHNARVQGKLVAPGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPY2</accession>
    <name>D5GPY2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00012088001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430380">
        <property type="protein sequence ID" value="CAZ86575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842384.1">
        <property type="nucleotide sequence ID" value="XM_002842338.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPY2"/>
    <dbReference type="FunCoup" id="D5GPY2">
        <property type="interactions" value="277"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GPY2"/>
    <dbReference type="GeneID" id="9182319"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012088001"/>
    <dbReference type="eggNOG" id="KOG3771">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025518_0_1_1"/>
    <dbReference type="InParanoid" id="D5GPY2"/>
    <dbReference type="OMA" id="QEYDYYN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031097">
        <property type="term" value="C:medial cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990528">
        <property type="term" value="C:Rvs161p-Rvs167p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051666">
        <property type="term" value="P:actin cortical patch localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097320">
        <property type="term" value="P:plasma membrane tubulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07599">
        <property type="entry name" value="BAR_Rvs167p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000048">
        <property type="entry name" value="BAR adaptor protein RVS167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000189">
        <property type="entry name" value="BAR adaptor protein RVS167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046982">
        <property type="entry name" value="BIN3/RVS161-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47174">
        <property type="entry name" value="BRIDGING INTEGRATOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47174:SF1">
        <property type="entry name" value="REDUCED VIABILITY UPON STARVATION PROTEIN 167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03114">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00721">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51021">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="BAR" evidence="7">
        <location>
            <begin position="17"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="6">
        <location>
            <begin position="378"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="279"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="345"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="296"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="349"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ86575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="437" mass="49939" checksum="86D90FBB491D81D1" modified="2010-06-15" version="1">MSWKGFQRSVVRAPQSIKQKFNMGEVTKDAIYLDAERQFDELEKETKKLHDESKKYFDAINGMLDHQIEFSKSIAEIYKPISGRVSDPDSIKIEGNPEGIRACEEYEAIVNDLKVTLQPELEMIETRIIRPADELMEIIKVIRKTALKRDHKQLDYDRHRASLKKLQDKKDKTLKDEKAMYKMEGDVELATQEFDYYNELLKTELPKLFMLEREFIQPLFQSFYYMQLNVFYTLHERMQGINIGYFDLTLDVEAAFEKKRDGVQERAEALAIVRFKTTGQRASKPNPKFQKFGKSDTASVTSRRTTSLDMSRTADDATGTPPPPYTSTGIPASTEEKDFLRPAAATGVARSNSTGTNYSVVGKKAPPPPRPKPKNLSVEPEKAPALYDYEAQADGDLSFNTGDVIEIVQRTSNENEWWTGKLHGRTGQFPGNYVKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5GQ67</accession>
    <name>D5GQ67_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Nascent polypeptide-associated complex subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">GSTUM_00012219001</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Mel28</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="12">Part of the nascent polypeptide-associated complex (NAC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the NAC-beta family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430382">
        <property type="protein sequence ID" value="CAZ86660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842469.1">
        <property type="nucleotide sequence ID" value="XM_002842423.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GQ67"/>
    <dbReference type="FunCoup" id="D5GQ67">
        <property type="interactions" value="1171"/>
    </dbReference>
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    <dbReference type="KEGG" id="tml:GSTUM_00012219001"/>
    <dbReference type="eggNOG" id="KOG2240">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098726_3_0_1"/>
    <dbReference type="InParanoid" id="D5GQ67"/>
    <dbReference type="OMA" id="RMQQSVR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22055">
        <property type="entry name" value="NAC_BTF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.30:FF:000003">
        <property type="entry name" value="Nascent polypeptide-associated complex subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.30">
        <property type="entry name" value="Nascent polypeptide-associated complex domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039370">
        <property type="entry name" value="BTF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038187">
        <property type="entry name" value="NAC_A/B_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002715">
        <property type="entry name" value="Nas_poly-pep-assoc_cplx_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10351">
        <property type="entry name" value="TRANSCRIPTION FACTOR BTF3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01849">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01407">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51151">
        <property type="entry name" value="NAC_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0678">Repressor</keyword>
    <keyword evidence="10 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="9 12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NAC-A/B" evidence="14">
        <location>
            <begin position="34"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="123"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="125"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="138"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="162"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="173"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAZ86660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="184" mass="20310" checksum="135CD41EDF2BAE34" modified="2010-06-15" version="1">MDQAKLARLQASVRIGMLLSLAYAIVKKVHKNAGTDDKKLQTSLKKLNVQPIQAIEEVNMFKEDGNVIHFAAPKVHASVPANTFAIYGNGEDKELTELVPGILNQLGPDSLASLRKLAESYQSLQKKEGEEGEKKEGDSDDDDDDDNDIPELVEGKNFDKDDKEEEKEEEKDEGGAEDAKPETE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5G3V3</accession>
    <name>D5G3V3_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Potassium transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00003817001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the TrkH potassium transport family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835075.1">
        <property type="nucleotide sequence ID" value="XM_002835029.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G3V3"/>
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        <property type="interactions" value="16"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G3V3"/>
    <dbReference type="GeneID" id="9187134"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003817001"/>
    <dbReference type="eggNOG" id="KOG1341">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005947_0_1_1"/>
    <dbReference type="InParanoid" id="D5G3V3"/>
    <dbReference type="OMA" id="MLRLMIW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140107">
        <property type="term" value="F:high-affinity potassium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003445">
        <property type="entry name" value="Cat_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004773">
        <property type="entry name" value="K/Na_transp_Trk1/HKT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015958">
        <property type="entry name" value="Trk1_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051143">
        <property type="entry name" value="TrkH_K-transport"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00934">
        <property type="entry name" value="2a38euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31064:SF30">
        <property type="entry name" value="HIGH-AFFINITY POTASSIUM TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31064">
        <property type="entry name" value="POTASSIUM TRANSPORT PROTEIN DDB_G0292412-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02386">
        <property type="entry name" value="TrkH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002450">
        <property type="entry name" value="K+_transpter_TRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0630">Potassium</keyword>
    <keyword evidence="4 10" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="72"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="420"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="488"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="547"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="615"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="673"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="697"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="116"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="294"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="353"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="167"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="193"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="211"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="295"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="316"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR002450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ79196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="809" mass="89848" checksum="70902000E01C2A66" modified="2010-06-15" version="1">MGLPRRWQFKPHFNFITVHYAYVIGMAIIGSVIIYPYKNMRYIDALFFAAGGATQSGLNTIDANLLKLYQQVVIYFLPMLTTPIFINTLVVFVRLYWFEKRFENIVSLTRQPSRARSALSKGTLDDNVESGREGAGVRGREITVLRAEPGTPLNLSKQSLSPGPGKNTEDRPRSMDSDIAPESVRSQTLQGRSSSDGVHGSDSSGVASSATEGPQTQRQNSEATGIQFVDLPSPSRDGAGCMSAFTSPHIAFTETQRNCVHGKALRIPGPREFEKGCRAQEIDEDDIGTLHRSRTTAEGDRNHDVGSPASRTMSLGNGVNGSNNVRQRKLPPLEQFLPRASTVERVISSAFSVGQHPKRRNTSPFSRLSRKTAKEPPNMPYLSYTPTLGRNSQFVGLSDEERDELGGIEYRSLKLLAKVLVGYYLFFHVFGVVCFTPWIWASGGYMDYVRSIGVNPTWWAIFSAQTAFNDLGFTLTPDSMVSFQSATFVLIIMTFLIIIGNTAFPCMLRLIIWIMFKLCPKESSHRESLNFLLDHPRRCFTLLFPSWPTWMLFWIVIVLNCADIILFIVLDLNDGDVTEIPVGHRIMGAIFQAASTRTAGLAVVNLADLHPAVQVSYMLMMYISVFPIAISVRRTNVYEESSLGIYANTDDEDNGRGASFVGAHLRKQLSFDLWYIFLGLFIITISEGSRIQDPKDYAFTTFAILFEVVSAYGTVGLSLGYPNSNASFSAQFGVIGKLVIIAMEIRGRHRGLPYELDRAILLPSESLQQKEAQDAQLRALRRRGSSFTQVGGRPLTGVRSVQSQAIGDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>D5G406</accession>
    <name>D5G406_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00003894001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835128.1">
        <property type="nucleotide sequence ID" value="XM_002835082.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G406"/>
    <dbReference type="GeneID" id="9182376"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003894001"/>
    <dbReference type="eggNOG" id="ENOG502QZVV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006918_0_0_1"/>
    <dbReference type="InParanoid" id="D5G406"/>
    <dbReference type="OMA" id="HYNITKL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059065">
        <property type="entry name" value="Ig_Tag1-like_4th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059066">
        <property type="entry name" value="Ig_Tag1-like_5th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046368">
        <property type="entry name" value="Tag1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055011">
        <property type="entry name" value="Tag1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35895">
        <property type="entry name" value="CHROMOSOME 16, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35895:SF3">
        <property type="entry name" value="PRE-RRNA PROCESSING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26174">
        <property type="entry name" value="LEA-2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26150">
        <property type="entry name" value="LEA-2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26153">
        <property type="entry name" value="LEA-2L_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22786">
        <property type="entry name" value="Tag1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="Tag1 C-terminal" evidence="3">
        <location>
            <begin position="452"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="domain" description="Tag1-like fourth Ig-like" evidence="4">
        <location>
            <begin position="572"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="domain" description="Tag1-like fifth Ig-like" evidence="5">
        <location>
            <begin position="712"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF26150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF26153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ79249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="839" mass="90314" checksum="5D572E09E1571DF6" modified="2010-06-15" version="1">MFPRNPPTHAPASSTERTSLLSNSSETPPVNYAEDPSRPSNAVATEHSGTPRLIRLDLIVLVFLCVSAISVLLLGFFAPAAAAQYAKEAVVLDITHLSVDSFTDNGVKVRVQASVQMDASRVKGQAVRSLGRTGTWIVNQISIDATNVEVYLPDYDGGLLVTASTPPIVLPVRNGQTTKLDFVSDVKLGSRDTVRSLANDYLVGGLGKIRVLGVADLGLKSGLLHLRTMKISEEMAFEGSRYKLTYGQSELPSLPEFQVNRMDVGEITLPGHKALSVNVSTVLQNPYPLEFDVPPLSFVILLSGCKADDLVNLATAETSHLSVESKTDVGVDVLGIVRNIPEELVTVCPGSHTSPIDIFLGTYLHGKDTTVYIQGSQSPTSNAPSWLLEFLQTITLPAPFPGHSFDSVVKSFSLSGVKFQLPSPNAPKGTPEASLRLSADVLATVRLPQKMTFPLDVSRLKATADATYEGHRFGTLHFPKWIPASSATSNDSKYLYVQAEVRDSLVDIEDYHIFDKIVRKLLSGDGKSIFLGINGSTDVGIGTIIGQFVVRNIPASGEIKIDGLPTTQMPLPKVNEVLIVDTTTQSATLGIGLSLENPTPWEIIVPHMSVHIMYDGFVLGNASIVNGHLMAGINSLSIGATWDPQAHGGHMAEEAGKRLAGKYVSGYNTTITLHPHTNSIPSMPLLSKLLSNFNVTMPTPRLPVPRNGGASDSPPTFIDSANIHLISSTADFLVNNPLRKDEVTVEAINGTAKYRCNELGEIESSKPFAIKPAVEGRTITPRIPVRWKIGGVGYEAMRKALGGRLAVHAEALCLVRVGKLQMEIFYNSTNPIGANIHLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>D5G440</accession>
    <name>D5G440_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00003939001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835162.1">
        <property type="nucleotide sequence ID" value="XM_002835116.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G440"/>
    <dbReference type="STRING" id="656061.D5G440"/>
    <dbReference type="GeneID" id="9182410"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003939001"/>
    <dbReference type="eggNOG" id="KOG1161">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015643_0_0_1"/>
    <dbReference type="InParanoid" id="D5G440"/>
    <dbReference type="OMA" id="FAFKQNA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033254">
        <property type="term" value="C:vacuolar transporter chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016237">
        <property type="term" value="P:microautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006799">
        <property type="term" value="P:polyphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14474">
        <property type="entry name" value="SPX_YDR089W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.100.30">
        <property type="entry name" value="VTC, catalytic tunnel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051572">
        <property type="entry name" value="VTC_Complex_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018966">
        <property type="entry name" value="VTC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042267">
        <property type="entry name" value="VTC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46140">
        <property type="entry name" value="VACUOLAR TRANSPORTER CHAPERONE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46140:SF1">
        <property type="entry name" value="VACUOLAR TRANSPORTER CHAPERONE COMPLEX SUBUNIT 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09359">
        <property type="entry name" value="VTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="725"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="765"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="798"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="domain" description="SPX" evidence="8">
        <location>
            <begin position="1"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="531"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="531"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="546"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ79283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="825" mass="91855" checksum="4D331535900B9F26" modified="2010-06-15" version="1">MKFGEALPQRSVQEWLPYNLDYNEIKQLIKRYTTGKPLVKSPEAKTDFEDEFFNILMDQLGRIDLFVKSKSGEVDRLRCKRQVAALRRRDTEDSALHHIRRPGRSTKLEQEVERVSYEVQCLSRFVNVQRTGFLKLLKKYKKWTGSSHLLNRFLPILKSPTAFHKVDFEKNVLELSNLLNAIRNGLDLPAAAPSPQIVTQPKRIRGAEQEDPQDRALSIETVLSQKAIATAEGRAYLDTALATSTLGDSCGGRATFWVHYDHLIELQVLLLKYLTLQTSPAYNAPPTPPSRKDSIGTLWKDDEIGVVLLDDLESFSDIQSSATVAEASKACSRTAGQVRWCCRDSDAVVVVSNASALILNKIAGHEEEKLEVRMKRKQIEELLDTGSISSRADINHTTNEQGETGVEKLEEWLAEHKNVTPLVKLLMKRIRFAETPEGGRVWALLDWDIKMTKVQQGTNWLTRPEEDGPGFGSEGRREFPHAVLEVQWEGQRVPTFLHELETSHMVENVRGFSLDVHALSLSALQRDIRKTPVHTPRHSRRSSSHMMFTSAASKSTSNGTSAAMAHDFADGFDSSTTAFSHTAPTVLPKAKSNRPKSGLSSIEHIGGERYWSEFNDDEDDEEPYTILIKRGDTSDDDCEDEDVSLSDFIRKSVFSTGHKFQALFTASPKHSGERKPLLRKPSVPSSESSSEIDVEVGAFGTGASSRAHYNTFSRPATINTPLSPTYVFCLGGAVVILIVTLILAMNSSLARHKHKWRRGHFTLDLEVCGAVLTALSLASIGLFIFLKSRSRAGPTHQMFAWGSFGAVCIGSGVVLAMVGNREVGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>D5G463</accession>
    <name>D5G463_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00003966001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835185.1">
        <property type="nucleotide sequence ID" value="XM_002835139.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G463"/>
    <dbReference type="STRING" id="656061.D5G463"/>
    <dbReference type="GeneID" id="9182433"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003966001"/>
    <dbReference type="eggNOG" id="ENOG502S4HG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033083_0_0_1"/>
    <dbReference type="InParanoid" id="D5G463"/>
    <dbReference type="OMA" id="YYVQLAT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WW" evidence="2">
        <location>
            <begin position="22"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="113"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="187"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="239"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="252"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="281"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="297"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="311"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="333"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="374"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="391"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="411"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ79306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="421" mass="40914" checksum="59CA054FFA71254D" modified="2010-06-15" version="1">MAPSPPNPAASPNATGPSFSPPHLPHGWIAQWDPKTQRYYYVQTSTAHTTWDVPTSSAPSTPAPSTPSSYPAPPTDYTTGGQGPVQPGYSNPSPQPDGQTSARAGGLGGIAGGLAQNLLSGGKTSHGSSGGGLGSLASGLLGGGKHSASGHGSSGHGSGGHGSGGGGLLGQASTIAGGLLGGKKPHGSSPGHSTSTHGSSSHGSTGHGSPSGGLGQLGGIVGGLLGGGAHGAQQTHGQGSHGQPGQQGLGYHGSSPSPNPSNPSYNANPHAPHQQYYHTGAPNQQFSGTVGQQNPASYGAPSPQGGYPAPGQYPPPLQNYPPPPVQYGAPSPAQYNPSTYQASPGGPDRPGGPGSYPPHSGPGGYQSPPPTSGPPSYGAPGSYQGGPFNPSAPTGGSYQGTPPPSQYQPTGGSGYHPPGGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4C1</accession>
    <name>D5G4C1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00004043001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835243.1">
        <property type="nucleotide sequence ID" value="XM_002835197.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4C1"/>
    <dbReference type="GeneID" id="9184989"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004043001"/>
    <dbReference type="HOGENOM" id="CLU_2887451_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4C1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="63" mass="6975" checksum="9A64D54B40A42E2D" modified="2010-06-15" version="1">MLIHGFSGAVHLYKYNTVQIGIRVLYSTAPDTFQSSPATSPLQVPYSPSQDRRLPFVLPPDQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4F7</accession>
    <name>D5G4F7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00004089001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835279.1">
        <property type="nucleotide sequence ID" value="XM_002835233.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4F7"/>
    <dbReference type="GeneID" id="9185025"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004089001"/>
    <dbReference type="eggNOG" id="ENOG502SD3B">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112553_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4F7"/>
    <dbReference type="OMA" id="GNWEADQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053203">
        <property type="entry name" value="Cisplatin_resist-associated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022024">
        <property type="entry name" value="DUF3602"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34693:SF5">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34693">
        <property type="entry name" value="PROTEIN PAR32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12223">
        <property type="entry name" value="DUF3602"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="176" mass="19146" checksum="DFD111ED8023F507" modified="2010-06-15" version="1">MSSEYVWTGRGGAGNMISKEELQKKEEDLEAQQSKATGSANQLAADEVAAPPPGEYKHMGRGGAGNWFQPSELVAQGATITDTMSVTPTPEPVAEKQRPPFLRPWRGRGGAGNFAPDSEEVQAAEKEELERNKLQRQLEEADVRASVDSTLSKPSQAHLKTGEKRWDVTGWKEAGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5T6</accession>
    <name>D5G5T6_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Vacuolar protein sorting-associated protein 35</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00001472001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Plays a role in vesicular protein sorting.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the VPS35 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430001">
        <property type="protein sequence ID" value="CAZ79879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835722.1">
        <property type="nucleotide sequence ID" value="XM_002835676.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5T6"/>
    <dbReference type="FunCoup" id="D5G5T6">
        <property type="interactions" value="1244"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G5T6"/>
    <dbReference type="GeneID" id="9187557"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001472001"/>
    <dbReference type="eggNOG" id="KOG1107">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005836_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5T6"/>
    <dbReference type="OMA" id="YIRSREY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030906">
        <property type="term" value="C:retromer, cargo-selective complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.660:FF:000002">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.660">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 35, helical subcomplex Vps35-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005378">
        <property type="entry name" value="Vps35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042491">
        <property type="entry name" value="Vps35_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11099:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11099">
        <property type="entry name" value="VACUOLAR SORTING PROTEIN 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03635">
        <property type="entry name" value="Vps35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009375">
        <property type="entry name" value="Retromer_Vps35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR009375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ79879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="786" mass="89726" checksum="ECCA0740032BD00A" modified="2010-06-15" version="1">MEDQQILLQDALTIVRQQTALMRKCLETPGKLMDALKCSSTLVSELRTSSLGPKQYYELYMAVFDALRHLSLYLRESHPVNHLADLYELVQYAGNIVPRLYLMITVGTVYMSIEDAPVKEIMKDMMEMSRGVQHPIRGLFLRYYLSGQSRDYLPTGNGEGPEGNFQDSISFIITNFIEMNKLWVRLQHQGHSREREKRTQERKELQLLVGSNLVRLSQLVDLETYKNIILAPLLEQVVQCRDVLAQEYLLEVITQVFPDEFHLHTLDQFLSATARLNPNVNVKAIVIGIMDRLSAYAAREAEGIPEDVKLYEIFFKQVLNLVNAQNLPIQDIIALLVSLANLALNIYPDRLGISVLSEQSYTHKKVSEMANSADLHSPQCQQYILNLMLAPVKSYASLFTVLALPSYLPLLHTQSYPTRRSVAGVVAQNILKNQTKISTPEHAEGIFELLRVLIREGAQQQAGYPGAQAPRKSRDIETDETVEEQGRLARIVHLLCSDNNDTQFKLLQTARKAFKEGGDRIRYTTPALITSGIKLARRYKLREHYDNEWQTMSAALYKFLHQTVTSIYRVGVPDLCLRLFLFCGQVSDQTEFEEVAYEFFAQAFTVYEEAISDSRAQFQAVCVIANALHSTRNFSKENYDTLITKCAQYGSKLLKKPDQCRAVYLASHLWWAVEIPARSEDERSPLYRDGKRVLECLQRALRVADACMDTAVSVELFVEILNRYVYYFDRQNEAVTVKYLNGLIELIQSNLSSNDSSTTETPRKHFERTLDYIASRDFAGVVLDPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5Z1</accession>
    <name>D5G5Z1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Carbonic anhydrase</fullName>
            <ecNumber evidence="4 9">4.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00001688001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 9">Reversible hydration of carbon dioxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>hydrogencarbonate + H(+) = CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:10748"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="EC" id="4.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the alpha-carbonic anhydrase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.1" evidence="4 9"/>
    <dbReference type="EMBL" id="FN430005">
        <property type="protein sequence ID" value="CAZ79934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835777.1">
        <property type="nucleotide sequence ID" value="XM_002835731.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5Z1"/>
    <dbReference type="STRING" id="656061.D5G5Z1"/>
    <dbReference type="GeneID" id="9187612"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001688001"/>
    <dbReference type="eggNOG" id="KOG0382">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039326_0_1_1"/>
    <dbReference type="InParanoid" id="D5G5Z1"/>
    <dbReference type="OMA" id="VPREAQY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03124">
        <property type="entry name" value="alpha_CA_prokaryotic_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.200.10">
        <property type="entry name" value="Alpha carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041891">
        <property type="entry name" value="Alpha_CA_prokaryot-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001148">
        <property type="entry name" value="CA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036398">
        <property type="entry name" value="CA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023561">
        <property type="entry name" value="Carbonic_anhydrase_a-class"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018338">
        <property type="entry name" value="Carbonic_anhydrase_a-class_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952:SF265">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00194">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01057">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51069">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00162">
        <property type="entry name" value="ALPHA_CA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51144">
        <property type="entry name" value="ALPHA_CA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="6 9" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025094374" description="Carbonic anhydrase" evidence="9">
        <location>
            <begin position="22"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-carbonic anhydrase" evidence="10">
        <location>
            <begin position="41"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ79934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="359" mass="37551" checksum="13E538DB77B03C5A" modified="2010-06-15" version="1">MYTQQLVFVAAALSAPILSSANCMYGTSLFPRAEGATVEVSNFGYVGLQGPLNWAALSAENSACATSKVQSPINIDSTIQLAQEAPKMVIKDVEAAEFENLGTTVEVICNGTTTFGGKDFSLQQFHFHTPSEHRVAEEYFPLEIHMVHEAADGSGALVVIALTFQLSEDGATTELLSAVTENLAKIATPGTVTETGPLKFAEVINHVQTTPLFQYTGSLTTPPCAEGLTFLVTEKPLPLNVKTYNEIKSVVKFNSRYTQNSPGQENLIAVGASALAGAGGAVAGEDARNGTAAAGNATVVVKPKPSGGAAASTSAKGTATSKTAAAATGNAEVKVEGEHHCILGSCREVTKAKRFRRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>D5G613</accession>
    <name>D5G613_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00001721001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430005">
        <property type="protein sequence ID" value="CAZ79956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835799.1">
        <property type="nucleotide sequence ID" value="XM_002835753.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G613"/>
    <dbReference type="GeneID" id="9187634"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001721001"/>
    <dbReference type="HOGENOM" id="CLU_346531_0_0_1"/>
    <dbReference type="InParanoid" id="D5G613"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="480"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="560"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="616"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="684"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="733"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="483"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="560"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="577"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="684"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="736"/>
            <end position="747"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="815" mass="89058" checksum="665F8FE8C3A17993" modified="2010-06-15" version="1">MSATLQYQPLQDLRRYVSLLDMHPVDPETETDISLQAMVPGQLYGRWDKSETFRLPRSLWSPLSKAVGGACRSVASSKEVDPKDSISHSKETVPDPTRVPIPISDVSGTGVPRSILPHPGLRGIREETIISPGSVAEAVSSAYQYQARLVSADSFPLLKKTASIRSALGNGPELGSRRGAQYGSASDHFMSPKLPIGSPLTPPKGQQVGLIQQYIPTRQYTSRTWNSLAPTKQHDRIDLDPLYHFTISKCFECFQASPSKLRDNFTAGNSSTATSSPGLLANMPWVFTMFGRASAFHTSSPLKTETSAPIGGPGIEALIHPRHLAYFHQFLTKSVLKGIEGLPEAKGTACEIVKDGVALEEDNDGEHKIADLEPNVELDMHNRPISAHCRRGSTVATRMMEESDTAELIEVLASTFHENDNRVTELGQDTEECEVAETAPSGHRRTNSLRYAVRQRASLFKPTSVKSDFTFVTEVTASPPVKRKQDDNTEKASRKRTRDNSVEYSIASPRNSIASRLSILIDNAEQRQAKLNSPGRELILSPINTQCVQVTEHLITEPFLSNSIPDSPVTPTSFTESTARSSTHSVSSAGTSLTVPYSHASPSASSALLTKKAWEEEQLRGRQSARGQNESRFPCAPGSGLGRSRSTGGIDSERPTEYSVELTRRRSLSTNSMLEKYAPMFQQPTVTSAPESQNLEIGETPSSSGSSSFVEPTELEDSLISSPPTQPQATLLVPEIKPEDTKKDAAQKKGPRRISYLGQLKSHKSFTAKVTEPSTTVVVSETEDGANKKKKFQKLKNRVKGITRKVSGVMKGEKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5G625</accession>
    <name>D5G625_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00001736001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430007">
        <property type="protein sequence ID" value="CAZ79968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835811.1">
        <property type="nucleotide sequence ID" value="XM_002835765.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G625"/>
    <dbReference type="STRING" id="656061.D5G625"/>
    <dbReference type="GeneID" id="9187646"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001736001"/>
    <dbReference type="eggNOG" id="KOG1720">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2134">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007422_0_0_1"/>
    <dbReference type="InParanoid" id="D5G625"/>
    <dbReference type="OMA" id="GNEHWTK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140096">
        <property type="term" value="F:catalytic activity, acting on a protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14504">
        <property type="entry name" value="DUSP23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.30">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054498">
        <property type="entry name" value="2H-SAK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052732">
        <property type="entry name" value="Cell-binding_unc_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057023">
        <property type="entry name" value="PTP-SAK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021122">
        <property type="entry name" value="RNA_ligase_dom_REL/Rnl2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43883:SF1">
        <property type="entry name" value="GLUCONOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43883">
        <property type="entry name" value="SLR0207 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22547">
        <property type="entry name" value="2H-SAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13671">
        <property type="entry name" value="AAA_33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22784">
        <property type="entry name" value="PTP-SAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09414">
        <property type="entry name" value="RNA_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56091">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="2">
        <location>
            <begin position="382"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ79968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="906" mass="99228" checksum="A83FD3A565D30307" modified="2010-06-15" version="1">MASSLAPQFFTPLQSVPAPTNIRGVMMLTSPSITHLLELFHSQNEVRKNPQPPHIALLIKSEHRALPDDALATLPKVDTSRIHPLGVSSHSGVTFIPVLWTAGNAWRVKHGLPVKDFHITLSAKDEHDIDKSIYSIAAEGVEGNGFVNGVLVPKGLGVEVLDQLYLSSHIRNEEIASQLGWAEYIVTSHPESERGYLRLADLACRNAWWKVAMLSYYAAFARSSPLDGAAGRRREYYLRKIVSCGEHTEFGPFLTEEELGQISTALSSSQVKDSLLQPWPPTSGLELPEVQPGSLAITRIRYHIPPGPEFPQLPRFFSWIVPFAVAGMSTPRSGEDIRHLSGMGITHVITLTSETPLAKSWFNVRIRNTLIPVENYHPPTIQQTDRALRIILEEPFCNPDSPGATLVHCGGGKGRAGTVLACYLALYGFTPPRLRSTKDPPKLSAKQAIALLRTLRDGSIETDRQEKFIETYVSTAWKRYGGGEPLIGDGGLVPEPSSGTLEITGNIADPDMILLIGLPGAGKSEFTRLCRLRNPALTVLSPDAITQESPSTAGSASARKVCECAVGAFKKGTGSGGHRKVLLLDRCNPTSAERKYWVQLLTSGGEVVAVFLDYPSEVCRSRAENRSSHPTLPPYRAAGAISSIQAAMQAPTLTEGYSGIARVTSIRSARELADTLFQPLSMKKFPRTRHLLNLGSATRDDLIIPETDLPRYFSRSVTIEEKVDGANLGFSLSSDLSILVQNRSHYVNASDAAQFAQLDRWLGLHGPTLVSVLHRDPSLPERFVLFGEWVAALHTVHYITLPDVFLAFDLYDRLEDRFATPGILRSVLAGSGIHAVPILWEGGVLDEGELLRFLDRKSAFGEEAVEGVVVRWDSGERAKVVRSGFVAGRHWSKNTLVKNGVVEVHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>D5G684</accession>
    <name>D5G684_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ubiquitin-conjugating enzyme E2 2</fullName>
            <ecNumber evidence="1">2.3.2.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">E2 ubiquitin-conjugating enzyme 11</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">E2 ubiquitin-conjugating enzyme 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Ubiquitin carrier protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Ubiquitin carrier protein UBC2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Ubiquitin-protein ligase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Ubiquitin-protein ligase UBC2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">GSTUM_00001653001</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="14">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="1"/>
    <dbReference type="EMBL" id="FN430008">
        <property type="protein sequence ID" value="CAZ80027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835870.1">
        <property type="nucleotide sequence ID" value="XM_002835824.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G684"/>
    <dbReference type="FunCoup" id="D5G684">
        <property type="interactions" value="407"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G684"/>
    <dbReference type="GeneID" id="9187706"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001653001"/>
    <dbReference type="eggNOG" id="KOG0421">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030988_9_1_1"/>
    <dbReference type="InParanoid" id="D5G684"/>
    <dbReference type="OMA" id="PKDNHAV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd23791">
        <property type="entry name" value="UBCc_UBE2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000068">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2-20 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 14" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="16">
        <location>
            <begin position="25"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="13">
        <location>
            <position position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00080142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAZ80027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="171" mass="18935" checksum="FBED4A95B23B1A23" modified="2010-06-15" version="1">MDQSTETTNTSGPGKSTTTLNGGQSVSKRLQTELMQLMMSTTPGISAFPTDGNLQHWTGTINGPEGTPYESLTFKLSLEFPSNYPMAPPTVLFTTPMFHPNVDMSGRICLDILKDKWSAVYNVQSILLSLQSLLGEPNNKSPLNGQAAELWDKDPKEYKRLLMLRHKEAED</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6C0</accession>
    <name>D5G6C0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pre-rRNA-processing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00004416001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 5">Component of the RIX1 complex required for processing of ITS2 sequences from 35S pre-rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the RIX1 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 5">Belongs to the IPI1/TEX10 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430009">
        <property type="protein sequence ID" value="CAZ80063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835906.1">
        <property type="nucleotide sequence ID" value="XM_002835860.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6C0"/>
    <dbReference type="FunCoup" id="D5G6C0">
        <property type="interactions" value="214"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G6C0"/>
    <dbReference type="GeneID" id="9187434"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004416001"/>
    <dbReference type="eggNOG" id="KOG2149">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050252_2_0_1"/>
    <dbReference type="InParanoid" id="D5G6C0"/>
    <dbReference type="OMA" id="VWSAMSH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120330">
        <property type="term" value="C:rixosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024679">
        <property type="entry name" value="Ipi1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16056">
        <property type="entry name" value="REGULATOR OF MICROTUBULE DYNAMICS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16056:SF2">
        <property type="entry name" value="TESTIS-EXPRESSED PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12333">
        <property type="entry name" value="Ipi1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Pre-rRNA-processing protein Ipi1 N-terminal" evidence="6">
        <location>
            <begin position="140"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU368021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ80063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="371" mass="39092" checksum="91104CAE6B1E096B" modified="2010-06-15" version="1">MGASAKRKREKHKDFQKPKLKVGKAKAKADNFTNTSFKSKAITLTTQSLHTTAPSTTYLFAHHISLLTHHSATTRKESLSYLKAHLPAALSTTASGTPNAISNLPTTSSLVSSLLPLILDPSTSVRAQLLALLTLLPHTLLTLHTRKILLFVNSAMTHISPDIRVDSAKFLDWLLGASREEVFSAGGWGVCLRGLCGCLGFNGAATGSGSEGSSLIVSKVQDGKITLSHLAVLTKVLEVGLVERVDLEGEVGVGDRRARPDGLHVSYEAHKLPSLSNVYSSLGLFDTPGSVSKGGGLQDPEPEDRDSRISYLIGGAGRGVFDSLVLGLGRLKKGGGEMGRAAGKALVVLEAADGGGVVGRCPVGRLYDTVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6N8</accession>
    <name>D5G6N8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00002145001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430012">
        <property type="protein sequence ID" value="CAZ80181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836024.1">
        <property type="nucleotide sequence ID" value="XM_002835978.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6N8"/>
    <dbReference type="GeneID" id="9187207"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002145001"/>
    <dbReference type="HOGENOM" id="CLU_2623784_0_0_1"/>
    <dbReference type="InParanoid" id="D5G6N8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="78" mass="8911" checksum="2416BB35C9771C53" modified="2010-06-15" version="1">MIASSLPTHSPPPMTHPPDQLMLQICPSPEQETPINKENEKKKKKRSEQTGYNHSIPAPKKVKRKNSAKPRGDRRCER</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="94" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6Q1</accession>
    <name>D5G6Q1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8">Phosphoinositide phospholipase C</fullName>
            <ecNumber evidence="2 8">3.1.4.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00002161001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + H2O = 1D-myo-inositol 1,4,5-trisphosphate + a 1,2-diacyl-sn-glycerol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33179"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:203600"/>
            <dbReference type="EC" id="3.1.4.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="3.1.4.11" evidence="2 8"/>
    <dbReference type="EMBL" id="FN430012">
        <property type="protein sequence ID" value="CAZ80194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836037.1">
        <property type="nucleotide sequence ID" value="XM_002835991.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6Q1"/>
    <dbReference type="FunCoup" id="D5G6Q1">
        <property type="interactions" value="313"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G6Q1"/>
    <dbReference type="GeneID" id="9187220"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002161001"/>
    <dbReference type="eggNOG" id="KOG0169">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002738_1_0_1"/>
    <dbReference type="InParanoid" id="D5G6Q1"/>
    <dbReference type="OMA" id="HWQREMS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate phospholipase C activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0051209">
        <property type="term" value="P:release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="CDD" id="cd00275">
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        <property type="match status" value="1"/>
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        <property type="entry name" value="EFh_ScPlc1p_like"/>
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    </dbReference>
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        <property type="entry name" value="PH_PLC_fungal"/>
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        <property type="entry name" value="PI-PLCc_eukaryota"/>
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        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
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        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
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        <property type="entry name" value="C2_domain_sf"/>
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        <property type="entry name" value="EF-hand-dom_pair"/>
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        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
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        <property type="entry name" value="PH-like_dom_sf"/>
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        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
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        <property type="entry name" value="PLipase_C_Pinositol-sp_Y"/>
    </dbReference>
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        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE PHOSPHODIESTERASE BETA-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336">
        <property type="entry name" value="PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C FAMILY PROTEIN"/>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
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    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50008">
        <property type="entry name" value="PIPLC_Y_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 8" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="6 8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="EF-hand" evidence="12">
        <location>
            <begin position="393"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="PI-PLC Y-box" evidence="11">
        <location>
            <begin position="762"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="10">
        <location>
            <begin position="793"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="87"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="655"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1002"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="90"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="185"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="655"/>
            <end position="672"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00012368"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU361133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ80194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1024" mass="114188" checksum="85579228D3E2D976" modified="2010-06-15" version="1">MAKASPPRHRPTDLHIQPQCFNVQSNNILQAQLTASPSSSEPLSYRHSINSVPSLFSSPTTPSPTLNNDFQAGMAFWSLKTWPTGRSKHISSSSDLAHSNLSPTITTPSTAPSSIHTTDPPKTPTQPRSKTSLIRRVSNKLRRRASSSTPKDSEERSGPVLARLRSDSFNGGETGRLGIQKKDEPFEDEDHEQAIEGEEDGARTSTSVEASHPDESIGAIGVMIPDVLKDGTAMTRVTRKKKTQRIFSLDPTATRVTWDATKQSSRFYIDDIKEIRLGADARNYREEFQVSSEVEDRWATIIYAEPETGKLKTLHVIAPTIETLELWTATLDIVMQYRTEMMAGLSMPGENFVDAHWRNYMGSRGPAKAPDSNDEKLGFEDVERLCRRLHVNCSRQFLKDRFKQSDKDKSGFLNFVEFREFVRLLKQRDEIVDIWRDAVTDRKAGMSVDEFRTFLKEEQKVDIDSEPMYIDKIFRKFTRPPSSQQQATRRERSTVRMSMDAFSDFLLSSSYNPPLLSAPLGNNFDRPLNEYYISSSHNTYLLGRQVAGESSIEAYIRVLKRGCRCVEIDCWDGDDGRPIVTHGHTGTSDILFLDVITAIRKYSFVTSNLPVILSLEVHCDLEQQVEMAKILRSVLGDALVTEPFMTNTLILPSPNVKGSSRQDTLMSNNDFGSTEKRAGSTNNLPNSSPPLPKSGIPSETSSSTTLFCTSQIREIRKGSGKEGSIREAQCSVPHASLPIWISSQLLEFRPNRILASRSPDGLQINEAMFASEEDRGGYVLKPKELRGSRTSFDPPADANAATLTKNKKVTTFSVEIISAQQLPKPKDHKDKGIHPFVVVEVFSADDKAKDKSVAEGGIGVDSSDAKGISGLGAPQKRRTKVVKDDGFHPLFREKMVFKVTTKFEELIFVRFSVYSADDGDSSDRTLIATYTAKLLSLQQGYRHLPLYDLQGEQFLFSMIFVKINVSSVSLADREPLMRESAMEAFKSTAKSVLQRGFSANGSSVRHLDSQKIGKTGGQEPTTRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>D5G708</accession>
    <name>D5G708_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00004535001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430020">
        <property type="protein sequence ID" value="CAZ80301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836110.1">
        <property type="nucleotide sequence ID" value="XM_002836064.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G708"/>
    <dbReference type="GeneID" id="9182492"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004535001"/>
    <dbReference type="HOGENOM" id="CLU_943952_0_0_1"/>
    <dbReference type="InParanoid" id="D5G708"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="295" mass="33059" checksum="D70A91F8D60D5808" modified="2010-06-15" version="1">MNPLHIPPGLPVPMMQQQTAKKRRQKSTASARSSNVNINGGGSDPSTPSTIGPSTWTTTARKRLDSKMLSLKDSTVPPPSNATLALAIEKLRYFIIRALHPNLHSVEKGGDNCLGLSMGPRLSFNYDAYIHWIDSSQQEGDEATRRLAQVWFFYKMADILTMFCDDEKRRGEGGNEQGRLQRGYDKLLPICKRILDTDNRQYIRNLICSSRRFRQLCIHMVGSERAEIFVFFKGELTLTDLKDMTASAAKMDETTAMLDVVLDDYLTTVSNIEVPIGGELTGIKIDMWNGLLQLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7R3</accession>
    <name>D5G7R3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00004688001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430031">
        <property type="protein sequence ID" value="CAZ80556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836365.1">
        <property type="nucleotide sequence ID" value="XM_002836319.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7R3"/>
    <dbReference type="STRING" id="656061.D5G7R3"/>
    <dbReference type="GeneID" id="9181375"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004688001"/>
    <dbReference type="eggNOG" id="ENOG502S5A2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136352_0_0_1"/>
    <dbReference type="InParanoid" id="D5G7R3"/>
    <dbReference type="OMA" id="PNIVAAR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37849">
        <property type="entry name" value="YALI0E11605P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37849:SF1">
        <property type="entry name" value="YALI0E11605P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="71"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ80556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="118" mass="13209" checksum="F9BAA866AE828648" modified="2010-06-15" version="1">MLSQTSRTLLLRTPRLRTFTASSIYRQAATSATATPAAIPKKRPVGAFRGGLFGFLLGSTFSAGTAYFYWLEEYRVSNELLTEDVEALRNSIQRVEAYVRRLEENMEIQKSAPGKGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>D5G880</accession>
    <name>D5G880_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00002923001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430041">
        <property type="protein sequence ID" value="CAZ80723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836532.1">
        <property type="nucleotide sequence ID" value="XM_002836486.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G880"/>
    <dbReference type="GeneID" id="9188325"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002923001"/>
    <dbReference type="HOGENOM" id="CLU_704359_0_0_1"/>
    <dbReference type="InParanoid" id="D5G880"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="201"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="365"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="365"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="379"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="392" mass="42716" checksum="15B2E3EECA55B572" modified="2010-06-15" version="1">MCFSKGRFCKRHTSNRTQNPDVGEWLKPIKSDKHGTGPLPPTLPVLSVTKPKSIVMKESLGATSPTIAIESPDNLRIELNANGVKDSLSTALVDTPVGEVGRKAEKVKSIICEVQKSKDGQDEESVDAHLVNLDVSAESATANVAEKSIVEAAEETADPEEIATRNFDATRSDDLTTHVASGVGQENEEGKAESETFSCFNGDFTRDDGNEDSSKSNPATPEISDQWVECAKEVEGTEAERAAAEATETGAAKVSLKEVDITSLDPGPVFDFSDIGEVEMPDSALFHDMYADSIWSGSVVTRNPGILLARSRDYRRYGLEPPGDCIKDEEDTPELTYTVDGVPRAPGRFGRYIRLREWKTHNDSQSYYPTLQSTPSYDSRGKKLDRSGGGRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5G887</accession>
    <name>D5G887_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00002930001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430041">
        <property type="protein sequence ID" value="CAZ80730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836539.1">
        <property type="nucleotide sequence ID" value="XM_002836493.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G887"/>
    <dbReference type="GeneID" id="9188332"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002930001"/>
    <dbReference type="HOGENOM" id="CLU_2869275_0_0_1"/>
    <dbReference type="InParanoid" id="D5G887"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="64" mass="7893" checksum="89E736E845BB2EAF" modified="2010-06-15" version="1">MILVHDMIPLPFRLSYRIEPRRAPLFFPPLTYLTNQTGQLHERKRKKRRKEKKAWRFPSFDENP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8K6</accession>
    <name>D5G8K6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00002987001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oxidase-dependent Fe transporter (OFeT) (TC 9.A.10.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430047">
        <property type="protein sequence ID" value="CAZ80849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836658.1">
        <property type="nucleotide sequence ID" value="XM_002836612.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8K6"/>
    <dbReference type="FunCoup" id="D5G8K6">
        <property type="interactions" value="54"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G8K6"/>
    <dbReference type="GeneID" id="9186217"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002987001"/>
    <dbReference type="eggNOG" id="ENOG502QQWE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046738_1_1_1"/>
    <dbReference type="InParanoid" id="D5G8K6"/>
    <dbReference type="OMA" id="SVWHLDC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033573">
        <property type="term" value="C:high-affinity iron permease complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061840">
        <property type="term" value="F:high-affinity ferrous iron transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033215">
        <property type="term" value="P:reductive iron assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004923">
        <property type="entry name" value="FTR1/Fip1/EfeU"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31632">
        <property type="entry name" value="IRON TRANSPORTER FTH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31632:SF2">
        <property type="entry name" value="PLASMA MEMBRANE IRON PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03239">
        <property type="entry name" value="FTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0410">Iron transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="51"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="87"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="139"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="203"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="288"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="318"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="332"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ80849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="359" mass="39144" checksum="046D316416F37881" modified="2010-06-15" version="1">MSNVFAVPVFFVVFRETIEAGIIVSVLLAFLKQTLGTEDEAIYKRLTRQVWIGLFAGLFICLVLGAAFIGAFYSIGKNLWEQGEDLWEGIFCLLACIIITIMGAAILRISKLRAKWQIKITEALELKKSRTLGSWMKKYVMIILPFVTVIREGLEAIVFVGGVSLSAPASSYPLPVVIGMVAGVAVSWGIYRGGNSLRIQWFLIASTMLLYLVAAGLGSRAVWKFEMYEFKKLVGGDVGETGSGPGSYDIRNTVWHVNCCSPGSRGGAGWGIFNAILGWQNTATYGSILSYNIYWIIVSLGFILMLFREKRGHYPLMKTPASNRASEEGSESGAGGVMTGGAPGEKMTPNTKLEVTDKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8Q3</accession>
    <name>D5G8Q3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00003085001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430050">
        <property type="protein sequence ID" value="CAZ80896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836705.1">
        <property type="nucleotide sequence ID" value="XM_002836659.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8Q3"/>
    <dbReference type="STRING" id="656061.D5G8Q3"/>
    <dbReference type="GeneID" id="9186264"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003085001"/>
    <dbReference type="eggNOG" id="ENOG502S1NM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035241_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8Q3"/>
    <dbReference type="OMA" id="PEPTMMA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14688">
        <property type="entry name" value="bZIP_YAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050936">
        <property type="entry name" value="AP-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40621:SF9">
        <property type="entry name" value="MEAB PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40621">
        <property type="entry name" value="TRANSCRIPTION FACTOR KAPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="5">
        <location>
            <begin position="53"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="134"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="72"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="137"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="148"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS00036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ80896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="294" mass="32795" checksum="DC4DE4D7C3ADDF25" modified="2010-06-15" version="1">MAAAVKASPPHSSASSASSASSPEPTMMAQQAQFPTPRRKGGRKPVYATQEERKMRNRAAQAAFRERRTEYIKHLEATIKHQEESLSSLQQSSRNAADEVLMLRYKNSLLERILLEKGINVAAELKAFAQYDDRPQPAAPQPIPRPIPSQAQQQQATLQQQQHMHRPMMAQPQQQQKRPSVNPPPESLFIKTSPIMPPNSRNNSPSIAIPTPPDASAYQIPQSVVTTPTAPSEFQHPGIPMQNFYPSPYQAHMEELGKLPRSRNMTPNYSRAQKTTLKADSKINLQRQTGLAVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8X9</accession>
    <name>D5G8X9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00004890001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430053">
        <property type="protein sequence ID" value="CAZ80972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836781.1">
        <property type="nucleotide sequence ID" value="XM_002836735.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8X9"/>
    <dbReference type="GeneID" id="9181255"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004890001"/>
    <dbReference type="HOGENOM" id="CLU_1455407_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8X9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="186" mass="19917" checksum="E086138747DC83E4" modified="2010-06-15" version="1">MAAVSQTPTSPPPQSLTSRGRRPALIPHNADLSVSLSAASAALGMASYSPNRERQRSGGVLWVVPGSAPPLPPPPQWDPQQSRGTGGTAEWDQDAMDVDDDDDDERMERELIRGLVAKDVFSWVNSTDAENNQAYSVASRSGGKSTCASSGPDTAPTSPLSGNFPEQLEFRRRRLEEAARESAGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>D5G929</accession>
    <name>D5G929_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="11">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00004959001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430053">
        <property type="protein sequence ID" value="CAZ81022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836831.1">
        <property type="nucleotide sequence ID" value="XM_002836785.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G929"/>
    <dbReference type="FunCoup" id="D5G929">
        <property type="interactions" value="432"/>
    </dbReference>
    <dbReference type="GeneID" id="9182353"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004959001"/>
    <dbReference type="eggNOG" id="KOG0764">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015166_6_1_1"/>
    <dbReference type="InParanoid" id="D5G929"/>
    <dbReference type="OMA" id="AFYNGMG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015218">
        <property type="term" value="F:pyrimidine nucleotide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990519">
        <property type="term" value="P:pyrimidine nucleotide import into mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000075">
        <property type="entry name" value="Nicotinamide adenine dinucleotide transporter 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049562">
        <property type="entry name" value="SLC25A33/36-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45829:SF1">
        <property type="entry name" value="CARRIER PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G06780)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45829">
        <property type="entry name" value="MITOCHONDRIAL CARRIER PROTEIN RIM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 10" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="8"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="111"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="213"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ81022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="313" mass="34320" checksum="176D8505575110D1" modified="2010-06-15" version="1">MAPPISSTKAEDSTSAGLASGILVCPLDVIKTKLQAQGGFSGMVDRATMGERVLAYRGLMGTARTIWAEEGVRGMYRGLGPLILGYLPTWTVYFTVYEKSKVVIADQFGGASWLTHILSAMVAGTSSTLVTNPIWVIKTRLMSQNANIPYHYTSTLDAARKMYLHEGIGSFYSGLAPALLGLSHVAVQFPLYEAFKGFFIGREHLERGSNGFTHFWSILAASCLSKICASSATYPHEVLRTRLQTQKVTHSDGDTRPRYRGIIHSARTVYREEGWRAFYAGMGTNMLRAVPASAMTLITYESLFSLLISYSLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5G947</accession>
    <name>D5G947_TUBMM</name>
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        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00003124001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430054">
        <property type="protein sequence ID" value="CAZ81040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836849.1">
        <property type="nucleotide sequence ID" value="XM_002836803.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G947"/>
    <dbReference type="GeneID" id="9184327"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003124001"/>
    <dbReference type="HOGENOM" id="CLU_2484955_0_0_1"/>
    <dbReference type="InParanoid" id="D5G947"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="87" mass="9550" checksum="7200D611642945D1" modified="2010-06-15" version="1">MPEPSLHTTGERHKPKPRVTTGDTKPQTHNTTSTAISTSFLPSPLPSSPTTPSNRLFSTLPAPLHPPVNHLFPLPLKNTRKPTKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9E5</accession>
    <name>D5G9E5_TUBMM</name>
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        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S26</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00003271001</name>
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    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS26 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430060">
        <property type="protein sequence ID" value="CAZ81138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002836901.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9E5"/>
    <dbReference type="FunCoup" id="D5G9E5">
        <property type="interactions" value="702"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G9E5"/>
    <dbReference type="GeneID" id="9182888"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003271001"/>
    <dbReference type="eggNOG" id="KOG1768">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129451_1_0_1"/>
    <dbReference type="InParanoid" id="D5G9E5"/>
    <dbReference type="OMA" id="KCYCVSC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1740.20:FF:000001">
        <property type="entry name" value="40S ribosomal protein S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1740.20">
        <property type="entry name" value="Ribosomal protein S26e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000892">
        <property type="entry name" value="Ribosomal_eS26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047864">
        <property type="entry name" value="Ribosomal_eS26_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038551">
        <property type="entry name" value="Ribosomal_eS26_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12538">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12538:SF0">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01283">
        <property type="entry name" value="Ribosomal_S26e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00733">
        <property type="entry name" value="RIBOSOMAL_S26E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="88"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="111"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ81138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="122" mass="13829" checksum="F4AC01B5422AC027" modified="2010-06-15" version="1">MVKKRASNGRNKKGRGHVKPIRCSNCSRCTPKDKAIKRFTIRNMVESAAIRDISDASVFPEYTVPKMYLKLQYCVSCAIHGKIVRVRSREGRRNRAPPPRIRYNKDGKKINPNQAAAKTTTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9M3</accession>
    <name>D5G9M3_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein-L-isoaspartate O-methyltransferase</fullName>
            <ecNumber evidence="7">2.1.1.77</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00003326001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>[protein]-L-isoaspartate + S-adenosyl-L-methionine = [protein]-L-isoaspartate alpha-methyl ester + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:12705"/>
            <dbReference type="Rhea" id="RHEA-COMP:12143"/>
            <dbReference type="Rhea" id="RHEA-COMP:12144"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90596"/>
            <dbReference type="ChEBI" id="CHEBI:90598"/>
            <dbReference type="EC" id="2.1.1.77"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.77" evidence="7"/>
    <dbReference type="EMBL" id="FN430063">
        <property type="protein sequence ID" value="CAZ81216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837025.1">
        <property type="nucleotide sequence ID" value="XM_002836979.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9M3"/>
    <dbReference type="STRING" id="656061.D5G9M3"/>
    <dbReference type="GeneID" id="9185352"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003326001"/>
    <dbReference type="eggNOG" id="KOG1661">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055432_0_4_1"/>
    <dbReference type="InParanoid" id="D5G9M3"/>
    <dbReference type="OMA" id="HMHASAC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004719">
        <property type="term" value="F:protein-L-isoaspartate (D-aspartate) O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000682">
        <property type="entry name" value="PCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00080">
        <property type="entry name" value="pimt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579">
        <property type="entry name" value="PROTEIN-L-ISOASPARTATE O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579:SF0">
        <property type="entry name" value="PROTEIN-L-ISOASPARTATE(D-ASPARTATE) O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01135">
        <property type="entry name" value="PCMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01279">
        <property type="entry name" value="PCMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 7" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ81216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="222" mass="24191" checksum="1CA4890A629C582F" modified="2010-06-15" version="1">MSWRCSSTSNEGLISNLFRHGLIKNDTVDRAHFSPIMPYEDSPQRIGYDATISGISFHYKNHAAEALLDRLGPGKTVLDVGSGSGYLTAVLAHLVAPGGVVVGIEHIQQLCDLSTENLKKDPVHSRMLQDGTIKIIRGDGRLGYPEGGPFDAIHVGAAASIMPQALIDQLKAPGRMFIPVDGGFSQYIWQVDKDEGGNVKKEKQYGVLYVPLTDAKDFRDEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9Y1</accession>
    <name>D5G9Y1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00003436001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430065">
        <property type="protein sequence ID" value="CAZ81324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837133.1">
        <property type="nucleotide sequence ID" value="XM_002837087.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9Y1"/>
    <dbReference type="STRING" id="656061.D5G9Y1"/>
    <dbReference type="GeneID" id="9188565"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003436001"/>
    <dbReference type="eggNOG" id="KOG1840">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_125_15_1"/>
    <dbReference type="InParanoid" id="D5G9Y1"/>
    <dbReference type="OMA" id="DQGKMSE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053137">
        <property type="entry name" value="NLR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46082:SF6">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46082">
        <property type="entry name" value="ATP/GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13374">
        <property type="entry name" value="TPR_10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="458" mass="50343" checksum="93E79CD13CC7F4F1" modified="2010-06-15" version="1">MYLRVLASREKVIDQGHRDTTRSVGDLGVAPQGQVERGGAEVMHRRAFTGKDKTLGSHSANTLGAAPHDQKKKSNEVENTHRHGAEGGGNPLGPNHPDVLLGVIGLAVTLLAESNYEEAKVIYRHGLRAMEKALGPDHPVALDSMNTLGETLLDLGRYDEAEAMCRLALAGREKTLGADHAVTLTSANNLGRTLYHQRKYEEAEMIFQSTQAGRKKVLGLYHPDTLLSAVCLGVTLLDRKKYGEAEVVYRHALGAMEKALGPDHPDVLDNVNTLGETLLDLKNYSEAEVVCRRALVGREKKFGPDHRDTLLSANNLGLALRNQNKHHEAEISYRRAMIGAEKTLGLGHRDTLMCIYNLAGVLHKQRKYGEAEMMHRRALTGRERILGLGHRHTLRSLIKLGTVLHEKGEYGEAEIMYRRAVTEAERALGQGHPTTLKNFNLLSIFLQARATSAAGEAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5GA20</accession>
    <name>D5GA20_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00003503001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430070">
        <property type="protein sequence ID" value="CAZ81363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837172.1">
        <property type="nucleotide sequence ID" value="XM_002837126.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GA20"/>
    <dbReference type="GeneID" id="9187061"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003503001"/>
    <dbReference type="HOGENOM" id="CLU_2887458_0_0_1"/>
    <dbReference type="InParanoid" id="D5GA20"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ81363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="63" mass="7552" checksum="B819F525C1E12144" modified="2010-06-15" version="1">MLDRRPRLRYSKGRRHLQAEYKFMRAFNSIATFGVNRLTCNLRAERLLPRLARCRRRNSSDGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAX4</accession>
    <name>D5GAX4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00005346001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NUP186/NUP192/NUP205 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430086">
        <property type="protein sequence ID" value="CAZ81667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837476.1">
        <property type="nucleotide sequence ID" value="XM_002837430.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5GAX4">
        <property type="interactions" value="150"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GAX4"/>
    <dbReference type="GeneID" id="9187835"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005346001"/>
    <dbReference type="eggNOG" id="KOG1835">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002778_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAX4"/>
    <dbReference type="OMA" id="AYGFIEW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044611">
        <property type="term" value="C:nuclear pore inner ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006999">
        <property type="term" value="P:nuclear pore organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021827">
        <property type="entry name" value="Nup186/Nup192/Nup205"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31344">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31344:SF0">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11894">
        <property type="entry name" value="Nup192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ81667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1596" mass="178417" checksum="CB36BB4157FA4B52" modified="2010-06-15" version="1">MDEVEAARLLLRGQKESQVLDRNAFQSAVFLFHTRRQNILESLRLILSYALNDEIDPEIRRAAKQAANLLASPKAPAAASGSGSFAEKCISTMASVRASVQQLREKERHEQTLRLVPTPGFKEDLEVQVRFLMNQHEALASIVFYLVKLKRAAVSDFKNLLGVVKSFEKHDIFAFHHILPLYSFCTVLCDPDSPINFQQTVELHKYMLNEYKSAPWPLRFVQASVQIWWLSEFNGLCNDPSSAEPPDSATLDYFTHVFGPSKAALEGGAFEFMLAVSADVSAEWYLNSAKEDLHRFLQTRVAPLEDNKLLSVSFKSLMISQFEIFIECFISNMADLLKQMKTQEEEDVLLTQRTCGYDLERFYMMIHYTYLGRRNAGMAFWVDPESNLHGFIVWASTSHMPFMVATFSYMLASLALGVTAAEHTHRFLQDEITPGSKKNRRTQYLSWEQLFKEFQKYIEQLRIRHKALVPTNSYRTPAIPAETVAEPPPESSMMLDGYLRLIAQVAQESLASRHWLLHESPNFRIMVALFDLLALQASVQLWDSVFSAITAFLTEKTENLNSQVWIAIDNWALGTPAPPSNALQLGSISGKPFAQAGASEKLDAIVTAVHPAEGFARLMATLVSPPVEDSLLKDSLPFPETLGSSTRLSGIDPYIDFIAGTIFTNTSLQNLPPDLPLVPEKIDDPTERLTRAQYRIFRPALQSSCLEFIETCLISFNEDLLNIASSNVAVDVAIGASSLETYAKLHPFARVMEHLLTEKCFNVLYEIIKIGVDDLASAGAPMPVLRTILMTINILDLAMRMQSTYFEVVRPLVKRDESGMRKMNTISTGFPSIEEAILHHLDIVVCLGLYVGSAYQDVTLAALSLLEKLSVAPKLVSIMGSSLGQQARKNRLLGAIEQSPESNRILFNFIQQWEADVDSEETDMRYPQKFAILKLLDSSLASQPNDFTLAHMLLGFGWDEKEGISLSVSSGGIGSGVSLLHSLLTDVDARESDEAGLAYDGGLCRLKNDIFSVLKRLWKSPATSTEILYVLRANKLLFSQMLSEQIVQRQTIWDGIPFESDEFFTRGAAASFCNFISRRTSLFNYIALEIRQLSTQGASTGVSKYLSTLLGKTVLDGQSMTNLHILDLLDFLELDAPEPIPTPKITWFNDVNLSAFYQDDPFGNPSYHLPTIDQILLLRRNELGALGVIDNSNDDSVTLEMKALLDYLFAENQLRLFKGANLECLKAWSTLIAVMLEDCEYEPSAKTLFLLTALQSILPKLESYCSGDIVYAQELSSLAHTLTTHLTFDSKTFGKGRASDMANDRMYQLFRISLRCIQSPMATANLREDFYNIAYRYLKGMSDLSTQTNGLTRHNTQTIKASGDRLLEVLCNDAYAGEGQCKIVALLLLETLVGLAADEGSSYVVDTLMRQNFIVVIIDSLKRIGHDLRNIASKGDFPSFFHFFILNGKLIVTDIDYLMRSFKATTGFLVRVSQTRSGSGYVINAGLFQALRESRLFAVDPDLGVALQKYYDLLQDAMRVVTCVILSKGPQNQTAIKLGRQFITENRSVIMTVFKRHAGIGGQRLDDIGDLGELVTLFVLLISVTAFVEGEERSLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAZ1</accession>
    <name>D5GAZ1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Pyrimidine-specific carbamoyl phosphate synthase-aspartate carbamoyl transferase</fullName>
            <ecNumber evidence="6">2.1.3.2</ecNumber>
            <ecNumber evidence="5">3.5.1.2</ecNumber>
            <ecNumber evidence="19">6.3.4.16</ecNumber>
            <ecNumber evidence="4">6.3.5.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="30" type="ORF">GSTUM_00005367001</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="30 31" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="30 31">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="24">Multifunctional protein that encodes the first 2 enzymatic activities of the de novo pyrimidine pathway: carbamoylphosphate synthetase (CPSase; EC 6.3.5.5) and aspartate transcarbamylase (ATCase; EC 2.1.3.2). The CPSase-function is accomplished in 2 steps, by a glutamine-dependent amidotransferase activity (GATase) that binds and cleaves glutamine to produce ammonia, followed by an ammonium-dependent carbamoyl phosphate synthetase, which reacts with the ammonia, hydrogencarbonate and ATP to form carbamoyl phosphate. The endogenously produced carbamoyl phosphate is sequestered and channeled to the ATCase active site. ATCase then catalyzes the formation of carbamoyl-L-aspartate from L-aspartate and carbamoyl phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20013"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:32814"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="EC" id="2.1.3.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>hydrogencarbonate + L-glutamine + 2 ATP + H2O = carbamoyl phosphate + L-glutamate + 2 ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:18633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>hydrogencarbonate + NH4(+) + 2 ATP = carbamoyl phosphate + 2 ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:18029"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="18">In the 2nd section; belongs to the CarB family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="15">In the 3rd section; belongs to the metallo-dependent hydrolases superfamily. DHOase family. CAD subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="16">In the C-terminal section; belongs to the aspartate/ornithine carbamoyltransferase superfamily. ATCase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="17">In the N-terminal section; belongs to the CarA family.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.2" evidence="6"/>
    <dbReference type="EC" id="3.5.1.2" evidence="5"/>
    <dbReference type="EC" id="6.3.4.16" evidence="19"/>
    <dbReference type="EC" id="6.3.5.5" evidence="4"/>
    <dbReference type="EMBL" id="FN430086">
        <property type="protein sequence ID" value="CAZ81684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837493.1">
        <property type="nucleotide sequence ID" value="XM_002837447.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5GAZ1">
        <property type="interactions" value="1477"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GAZ1"/>
    <dbReference type="MEROPS" id="C26.956"/>
    <dbReference type="GeneID" id="9187852"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005367001"/>
    <dbReference type="eggNOG" id="KOG0370">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000513_2_1_1"/>
    <dbReference type="InParanoid" id="D5GAZ1"/>
    <dbReference type="OMA" id="WSPFNGK"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00115"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005951">
        <property type="term" value="C:carbamoyl-phosphate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004070">
        <property type="term" value="F:aspartate carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004087">
        <property type="term" value="F:carbamoyl-phosphate synthase (ammonia) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01744">
        <property type="entry name" value="GATase1_CPSase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01423">
        <property type="entry name" value="MGS_CPS_I_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1370:FF:000002">
        <property type="entry name" value="Aspartate carbamoyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000036">
        <property type="entry name" value="Aspartate carbamoyltransferase catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000025">
        <property type="entry name" value="Bifunctional pyrimidine biosynthesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1370:FF:000005">
        <property type="entry name" value="CAD protein-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000004">
        <property type="entry name" value="Carbamoyl-phosphate synthase (glutamine-hydrolyzing)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.20:FF:000002">
        <property type="entry name" value="Carbamoyl-phosphate synthase 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1030.10:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000036">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000002">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1380:FF:000009">
        <property type="entry name" value="Carbamoyl-phosphate synthase, large subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.20">
        <property type="entry name" value="Carbamoyl-phosphate synthase small subunit, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1030.10">
        <property type="entry name" value="Carbamoyl-phosphate synthetase, large subunit oligomerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1380">
        <property type="entry name" value="Methylglyoxal synthase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01209">
        <property type="entry name" value="CPSase_S_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006130">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002082">
        <property type="entry name" value="Asp_carbamoyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036897">
        <property type="entry name" value="CarbamoylP_synth_lsu_oligo_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006274">
        <property type="entry name" value="CarbamoylP_synth_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002474">
        <property type="entry name" value="CarbamoylP_synth_ssu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036480">
        <property type="entry name" value="CarbP_synth_ssu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005483">
        <property type="entry name" value="CPSase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035686">
        <property type="entry name" value="CPSase_GATase1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006275">
        <property type="entry name" value="CPSase_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005480">
        <property type="entry name" value="CPSase_lsu_oligo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058047">
        <property type="entry name" value="CPSase_preATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011607">
        <property type="entry name" value="MGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036914">
        <property type="entry name" value="MGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00670">
        <property type="entry name" value="asp_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01369">
        <property type="entry name" value="CPSaseII_lrg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01368">
        <property type="entry name" value="CPSaseIIsmall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002032">
        <property type="entry name" value="PRK00856.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003671">
        <property type="entry name" value="PRK05294.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009455">
        <property type="entry name" value="PRK12815.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009475">
        <property type="entry name" value="PRK12838.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405:SF5">
        <property type="entry name" value="CAD PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405">
        <property type="entry name" value="CARBAMOYLTRANSFERASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25596">
        <property type="entry name" value="CPSase_L_D1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02787">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00988">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02142">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00101">
        <property type="entry name" value="ATCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00098">
        <property type="entry name" value="CPSASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00099">
        <property type="entry name" value="CPSGATASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01096">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01097">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00851">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48108">
        <property type="entry name" value="Carbamoyl phosphate synthetase, large subunit connection domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52021">
        <property type="entry name" value="Carbamoyl phosphate synthetase, small subunit N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52335">
        <property type="entry name" value="Methylglyoxal synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00097">
        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51855">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 26" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0436">Ligase</keyword>
    <keyword evidence="14" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="10 26" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ATP-grasp" evidence="28">
        <location>
            <begin position="532"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="28">
        <location>
            <begin position="1067"/>
            <end position="1258"/>
        </location>
    </feature>
    <feature type="domain" description="MGS-like" evidence="29">
        <location>
            <begin position="1324"/>
            <end position="1505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="1814"/>
            <end position="1837"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00043968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00043979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00043984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00043998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00044063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00048859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00049534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00058513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00081752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS51855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="EMBL" id="CAZ81684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="2194" mass="242286" checksum="19409EA1CA756D4C" modified="2010-06-15" version="1">MTLELEDGSAYQGYSFGAEKSISGELVFQTGMVGYPESITDPSYRGQILVITFPLVGNYGVPDRETRDELLDSLPSYFESREVHIAGLITASYSGEDYSHYLAKSSLGTWLKERGVPAIYGVDTRTLTKKIRTQGISARSPIDRDWRQDFESQEWVDPNAKNLVAEVSIRGPQIFSPPVDRALKRPDGKPIRIVCVDVGMKFNQLRCFLSRGIEVKVVPWDYDFLKEEENYDGLFISNGPGDPATLKTTVDRLRTAIEVVKTPIFGICLGHQLLARAAGAETAKMKFGNRGHNIPCTNLRSGRCYITSQNHGFAVNAKSLKSGWQELFVNANDGSNEGIWHTQKPLFSVQFHPESTPGPKDTEWLFDVFISAIQKTQGPNGGAALSQPVEIPGGLIEDNERANPKVKVKKVLVLGSGGLSIGQAGEFDYSGSQAIKALKEEGIYTILINPNIATIQTSKGLADKVYFLPVTAEFVRKVIQYERPDSIYVTFGGQTALQVGIQLKDEFEGLGVKVLGTPIETIITTEDRELFARAMEEIGEKCAKSASASSVEEAMTVVKDIGFPVIVRAAYALGGLGSGFAYDEDQLRDLCNKAFAASPQVLIEKSMKGWKEIEYEVVRDCRDNCITVCNMENFDPLGIHTGDSIVVAPSQTLSDEDYNMLRTTAVNVIRHLGVVGECNIQYALNPFSKEYCIIEVNARLSRSSALASKATGYPLAFIAAKLGLGIPLNEISNSVTKVTCACFEPSLDYVVVKIPRWDLKKFTRVSTLLSSSMKSVGEVMSIGRTFEEAIQKAIRAIDYNNLGFNQTKALMSVDQELQTPSDQRLFAIANAMHSGYTVERIWELTQIDKWFLNKLMGLTNFGKLMCKYNASSIPTSLFRQAKQLGFSDRQLAEFWGSNEVAVRSLRVEEGIMPSVKQIDTVAAEFPAFTNYLYLTYNGVEHDVEFEDKGVMVLGSGVYRIGSSVEFDWCAVRAVRTLREMGIKTIMVNYNPETVSTDYDEADKLFFENINLETILDIYQLEQSSGVIIAMGGQTPNNIALPLHRLNVKILGTSPEMIDSAENRYKFSRLLDRIEVDQPAWKELTSIEEAEAFCDKVTYPVLVRPSYVLSGAAMNTVYSKDDLASYLRQAADVSREHPVVITKYIENAKEIEMDAVAKDGKMLMHFISEHVENAGVHSGDATLILPPQDLDPETVRRIEEATGKIGNALNVTGPFNIQFIAKDNEIKVIECNVRASRSFPFVSKVMGVDLIEMATKAILGFQVDAYPKIDIPKDYVGIKVPHFSFSRLSGADPVLGVEMASTGEVACFGRDKSEAYIKALMSTGFRLPKKNILLSIGSFKEKMEMLPSVKKLFQLGYKLFATAGTADFIQEHGVPVQYLEVLGEQDEPNGQKTEYSLTQHLANNLIDLYINLPSNNRFRRPASYMSRGYQTRRMAVDYHTPLVTNVKNAKLLVEALSRNHKLKIDSLDYQTSHRTITLPGLINIAAFVPGLAKEDSKDFELVTHASIASGFSMIRVLPLGVEGSLTDATALEAALANSKLAHCDFNYSVAATSTNSQQLSTLTSRVGSLFIPFNHLSGNINKVATVTSHFGVWPALKPIMTDAKTTDLASILLLASLHNRNVHVTSITTKDDIKLIALSKEKGLKVTCDVAVYALFLSQDDYPTCPVLPTAEDQKALWDNLAIIDCFSVGSLPFQLAHDMKQDVNAAFGIADTLPLLTTAICNGRLTIDDLKIRLHDNPKTIFDLHDQPTCSVEVEMDRSYKVQPGDVWSPFVDRTLKGAVQRVTFKGKTAALDGYISSDGSHGTAASVPNPVAATSVASPQLRPESSHSRQNLLSPRLAGRTQSSALDSLPALQTPSSLLVNHSEASPHFQLSAPMIPASLSELLSRDSMFKRRHILSVNQFSRADLHHLFTVAQEMRLAVERWGVIDLLRGRLLCTMFFEPSTRTSASFDAAMQRLGGRTVAISAAHSSLKKGETLKDTIRTLACYGDAVVLRHPAEDSAAEAARFSPVPIINGGNGSLEHPTQAFLDLFTIREELGTVNGLTITFVGDLKFGRTVHSLAKLLQHYHVRINFASPPQLSMPATVRDSLKKAGMEVKEYHELIPDLVASSDVLYCTRVQKERFADLNEFEKLKDSFIIDHSVLKNAKSHMVVMHPLPRNAEIGEEVDYDQRAAYFRQMKYGLYCRMALLALVLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>D5GB09</accession>
    <name>D5GB09_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00005393001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430086">
        <property type="protein sequence ID" value="CAZ81702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837511.1">
        <property type="nucleotide sequence ID" value="XM_002837465.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GB09"/>
    <dbReference type="STRING" id="656061.D5GB09"/>
    <dbReference type="GeneID" id="9182550"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005393001"/>
    <dbReference type="eggNOG" id="ENOG502S2WH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016578_0_0_1"/>
    <dbReference type="InParanoid" id="D5GB09"/>
    <dbReference type="OMA" id="HEVYRKQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23159">
        <property type="entry name" value="CENTROSOMAL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23159:SF31">
        <property type="entry name" value="CENTROSOME-ASSOCIATED PROTEIN CEP250 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="251"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="500"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="413"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="75"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="119"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="251"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="263"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="282"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="300"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="327"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ81702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="605" mass="66331" checksum="10C20D8F02B7D273" modified="2010-06-15" version="1">MADTDAAKAEKIAAAKRRFEQLKKQKGKKKGSVTPASTTGETSKSPVQTEQAEASEAPSPTEGQLVEENAKELAAEAGEVAETPAAAAEVVNSDASSPMTPADPESSHSPVASLGGAGAAAHSRAASIHARRQSASGFRRPSITAADIVRSSNKSPLSPGETVPDIYRKQATTISELTESVERLEAEVERLKEKEKKLSEVTVQKDEAQETLAGVKTELKEAVERAEAAATERKAREEELEKLKSEVATLTRQMSHLTSQISQKDKAISDLKLHSSSSSSTQDRESLTAKEDQIENMEMDISKLRGDLERANKSLAESQSALATAEGKVKGLEESQEDVAKELEENKKKLDKAAERLVAEGAARSSAETKITSVQAEFESASATVKSQAGSISSWEKDYNTLKTMYRDTDMKGQESQKKASALERENKNLTTQVLELRKRTTHLESENATLRSSLEKAISDARRGTGSMPQDDELVDELEDDGRKRLEAKVRQLEQQLQQERSRWYTSEKQGLASASAPPRSTQQHQQEFLDVDLNAGDGQHHPRGGGNDHLYAAQMEEERRQMEAQRLERIKEVKRGLANWKGWRLDMTAGGGYGVNFREMFEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5GB95</accession>
    <name>D5GB95_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00000528001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430089">
        <property type="protein sequence ID" value="CAZ81788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837597.1">
        <property type="nucleotide sequence ID" value="XM_002837551.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GB95"/>
    <dbReference type="STRING" id="656061.D5GB95"/>
    <dbReference type="GeneID" id="9181499"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000528001"/>
    <dbReference type="eggNOG" id="KOG1208">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_44_3_1"/>
    <dbReference type="InParanoid" id="D5GB95"/>
    <dbReference type="OMA" id="CNAFTMS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000253">
        <property type="term" value="F:3-beta-hydroxysteroid 3-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051593">
        <property type="entry name" value="Ergosterol_Biosynth_ERG27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43647">
        <property type="entry name" value="DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43647:SF4">
        <property type="entry name" value="KETOREDUCTASE (KR) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ81788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="334" mass="36554" checksum="4C7434E91BE5758B" modified="2010-06-15" version="1">MAPQGTVLITGANGTLATALVENLLINYPEHPLLLAVRNTSDSDVHTKNLRRLSSCHPSTPVHIEPLDLSSLASVRTFTTTVTSLILSGTIPPLAAIVCNAFSWSLNSGIQFTQDGYESTFQVCHLGHFLLVLRLLASMEGTGRVIIIGSEEHSDEKPSLISPYRPGIPEKVEELVKPAPEDAKTTFNRGFTRYATAKLCQTMFMYDLGRRLSQDPNLSGITVSVLDPGGMPASRCFVQHPTVVKLLMNYIFAPAVHVLKYVTNKFRTPWDASVDVADVAVGDLGKESGYYVCKRKIEGSCASRDDTLQGVLWRKSIEWTRLEKGETVLGSTFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBC0</accession>
    <name>D5GBC0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Histone H4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00000409001</name>
        <name evidence="15" type="ORF">GSTUM_00006686001</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 16">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the histone H4 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430090">
        <property type="protein sequence ID" value="CAZ81813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="FN430197">
        <property type="protein sequence ID" value="CAZ83101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837622.1">
        <property type="nucleotide sequence ID" value="XM_002837576.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838910.1">
        <property type="nucleotide sequence ID" value="XM_002838864.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBC0"/>
    <dbReference type="SMR" id="D5GBC0"/>
    <dbReference type="FunCoup" id="D5GBC0">
        <property type="interactions" value="1121"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GBC0"/>
    <dbReference type="GeneID" id="9184387"/>
    <dbReference type="GeneID" id="9188639"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000409001"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006686001"/>
    <dbReference type="eggNOG" id="KOG3467">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109117_2_3_1"/>
    <dbReference type="InParanoid" id="D5GBC0"/>
    <dbReference type="OMA" id="QKEHING"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22912">
        <property type="entry name" value="HFD_H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000007">
        <property type="entry name" value="Histone H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035425">
        <property type="entry name" value="CENP-T/H4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001951">
        <property type="entry name" value="Histone_H4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019809">
        <property type="entry name" value="Histone_H4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004823">
        <property type="entry name" value="TAF_TATA-bd_Histone-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10484">
        <property type="entry name" value="HISTONE H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15511">
        <property type="entry name" value="CENP-T_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00623">
        <property type="entry name" value="HISTONEH4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00417">
        <property type="entry name" value="H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00803">
        <property type="entry name" value="TAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00047">
        <property type="entry name" value="HISTONE_H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5 11" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="10 11" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TATA box binding protein associated factor (TAF) histone-like fold" evidence="13">
        <location>
            <begin position="28"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ81813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAZ83101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="103" mass="11370" checksum="9392CDB8AE0C52E2" modified="2010-06-15" version="1">MTGRGKGGKGLGKGGAKRHRKILRDNIQGITKPAIRRLARRGGVKRISAMIYEETRGVLKTFLEGVIRDAVTYTEHAKRKTVTSLDVVYALKRQGRTLYGFGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBF7</accession>
    <name>D5GBF7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00000450001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837659.1">
        <property type="nucleotide sequence ID" value="XM_002837613.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBF7"/>
    <dbReference type="GeneID" id="9184424"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000450001"/>
    <dbReference type="HOGENOM" id="CLU_3385070_0_0_1"/>
    <dbReference type="InParanoid" id="D5GBF7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ81850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="33" mass="3828" checksum="18415AA5651363FC" modified="2010-06-15" version="1">MIETDPSKYYIHSGILSQQCETENRGQYLIGPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBI3</accession>
    <name>D5GBI3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="13">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00005648001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ81989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002837752.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBI3"/>
    <dbReference type="FunCoup" id="D5GBI3">
        <property type="interactions" value="197"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GBI3"/>
    <dbReference type="GeneID" id="9185424"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005648001"/>
    <dbReference type="eggNOG" id="KOG4504">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064145_0_0_1"/>
    <dbReference type="InParanoid" id="D5GBI3"/>
    <dbReference type="OMA" id="RTKSTIM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.130.10:FF:000024">
        <property type="entry name" value="Putative vacuolar sorting receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.130.10">
        <property type="entry name" value="Mannose-6-phosphate receptor binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028927">
        <property type="entry name" value="Man-6-P_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009011">
        <property type="entry name" value="Man6P_isomerase_rcpt-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044865">
        <property type="entry name" value="MRH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15071:SF0">
        <property type="entry name" value="MANNOSE 6-PHOSPHATE RECEPTOR-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15071">
        <property type="entry name" value="MANNOSE-6-PHOSPHATE RECEPTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02157">
        <property type="entry name" value="Man-6-P_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01404">
        <property type="entry name" value="CIMR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50911">
        <property type="entry name" value="Mannose 6-phosphate receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51914">
        <property type="entry name" value="MRH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005671500" evidence="11">
        <location>
            <begin position="26"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="216"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="257"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="MRH" evidence="12">
        <location>
            <begin position="34"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="147"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ81989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="317" mass="34478" checksum="BE6B9E3453BD752C" modified="2010-06-15" version="1">MRFWIASPISALPFFVFSLLQLTIASTSNTPQQEPCTVISAQSGNFFDLRPLTRTSASTSSSSSSSNTVHDWTARGHDYPANFTLNICAPVLSDVADVVSVSTSQKSNISAFYITQEGKQYSIGSVSTNPYFRGRKLVLVYAGGSPCPAPDGEEREKSKSSKKKKGSGYRKSTIMSHLCDRDAVRPVISFVGEVNECGYFFEVRSSAACPTAGTQALGPVSVFGIIGLVAALVYLVGGCVYQRTVMHARGWRQMPNYAVWAGLVGGVWALIRSIWDRCASKLHLPRSSYDLIGARRSSLESENRLIDALDEDWGEDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBL7</accession>
    <name>D5GBL7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00005692001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ82023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837832.1">
        <property type="nucleotide sequence ID" value="XM_002837786.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBL7"/>
    <dbReference type="STRING" id="656061.D5GBL7"/>
    <dbReference type="GeneID" id="9185458"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005692001"/>
    <dbReference type="eggNOG" id="KOG4178">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_20_1_1"/>
    <dbReference type="InParanoid" id="D5GBL7"/>
    <dbReference type="OMA" id="MLTFRHG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050471">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43433:SF5">
        <property type="entry name" value="AB HYDROLASE-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43433">
        <property type="entry name" value="HYDROLASE, ALPHA/BETA FOLD FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="67"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="326" mass="36303" checksum="24CDA5327795A620" modified="2010-06-15" version="1">MSLPLEEIRGHPAWSTVIELQTPNWQGVAPVAKNRSGGPINIAYEVHGTGPIHTVVRLPRSTMFPGHEHKDKYTSLVFDNRGVGNSDKPYARYSTSEMAKDVIDLLQHVGWTDPNQLHVIGVSMGGMIAQELAFLIPERIASLCLQSTAACLVNSIPWYKHLYRRAYMLLPKSLPARLAATQENIFAAAWLKAPDDLGLFPTNSDRYVAEELWRVENLPPPVYQGFLLQGLAVSWHYMGPERLKVLGGRIRHILVCTGDLDAMIECKHSDVLVRGISAGGRVTKRVFEGSGHALHFECAREYNCMIEEFVTAAHEDGKGATINRWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5GC35</accession>
    <name>D5GC35_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">4-hydroxyphenylpyruvate dioxygenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">GSTUM_00000546001</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="12">Binds 1 Fe cation per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 3/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the 4HPPD family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430099">
        <property type="protein sequence ID" value="CAZ82078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837887.1">
        <property type="nucleotide sequence ID" value="XM_002837841.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GC35"/>
    <dbReference type="STRING" id="656061.D5GC35"/>
    <dbReference type="GeneID" id="9181347"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000546001"/>
    <dbReference type="eggNOG" id="KOG0638">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034004_3_1_1"/>
    <dbReference type="InParanoid" id="D5GC35"/>
    <dbReference type="OMA" id="DPFPVKG"/>
    <dbReference type="UniPathway" id="UPA00139">
        <property type="reaction ID" value="UER00362"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003868">
        <property type="term" value="F:4-hydroxyphenylpyruvate dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006559">
        <property type="term" value="P:L-phenylalanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006572">
        <property type="term" value="P:L-tyrosine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07250">
        <property type="entry name" value="HPPD_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08342">
        <property type="entry name" value="HPPD_N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.180.10:FF:000001">
        <property type="entry name" value="4-hydroxyphenylpyruvate dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.180.10:FF:000020">
        <property type="entry name" value="4-hydroxyphenylpyruvate dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005956">
        <property type="entry name" value="4OHPhenylPyrv_dOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041735">
        <property type="entry name" value="4OHPhenylPyrv_dOase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041736">
        <property type="entry name" value="4OHPhenylPyrv_dOase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC_core"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01263">
        <property type="entry name" value="4HPPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11959">
        <property type="entry name" value="4-HYDROXYPHENYLPYRUVATE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11959:SF1">
        <property type="entry name" value="4-HYDROXYPHENYLPYRUVATE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009283">
        <property type="entry name" value="HPP_dOase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="9 12" id="KW-0408">Iron</keyword>
    <keyword evidence="4 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-0585">Phenylalanine catabolism</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0828">Tyrosine catabolism</keyword>
    <feature type="domain" description="VOC" evidence="14">
        <location>
            <begin position="42"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="VOC" evidence="14">
        <location>
            <begin position="217"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="386"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR009283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR009283-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAZ82078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="418" mass="45915" checksum="5E274FED089BDA92" modified="2010-06-15" version="1">MPPSALSSSPPGPTILTSSGQTLPLDGVQSPAAALVTTGYRGYDHVHWYVGNAKQAASYYISRMGFQPLAYKALETGSRIVASHVVSNGGVTFVLTSPLRSPSTPGLTTADVEILKEMYEHLSTHGDAVKDVSFEVDDVRKVYESAIKNGARGVKAPEVLSDEDGEVIVATVATYGDTTHTLVQRGGYSGVFMPGFRRAAARQDPLDNILPAVHLDAIDHCVGNQDWGEMDNVCDYYEKALGFHRFWSVDDKTVCTEYSALKSVVMASPNELVKMPINEPAHGKRKSQIEEYVDFYSGSGVQHIALRTSNIIAAVSNLRARGVEFITIPDSYYKQMKVRLSKSSVRVKEDTELLKELGILIDFDEGGYLLQLFTKPLMDRPTVFIEIIQRCNFNGFGAGNFRSLFEAIERDQAERGNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>D5GC46</accession>
    <name>D5GC46_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00000561001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430100">
        <property type="protein sequence ID" value="CAZ82089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837898.1">
        <property type="nucleotide sequence ID" value="XM_002837852.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GC46"/>
    <dbReference type="STRING" id="656061.D5GC46"/>
    <dbReference type="GeneID" id="9181358"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000561001"/>
    <dbReference type="eggNOG" id="ENOG502QT9U">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006603_0_0_1"/>
    <dbReference type="InParanoid" id="D5GC46"/>
    <dbReference type="OMA" id="IAGIWMN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF23">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="317"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="329"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="341"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="359"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="409"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ82089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="709" mass="77212" checksum="D620A0BC3DFADFE7" modified="2010-06-15" version="1">MTSSPLMNHTTAKIFCHFIYVLGPSLSLFERHPPNPHVAFTPGAGLSGPQNVWSYTVPMLSLSHPPLLHAILALSSLHISKLTRGPTHPSLLHYHIALRRLGKAIASDKHRGHAATLTATLVLGYYETMAAEHDKWSSHIHGAKQLLKEIDFDKLNRRVSTMEEERADGGQGSYESAESALQPSLLRLRRMRRPPVAVANEADKNLTAFMMGKSKRRLYGSGKRPDAPFSRKEEETMQLQEDMFWWFAKMDCYQSVLSGCPLVLDYVFWSQCPPRARIGTLGTTYGSSDHFFLLLGRLCDFQVRDLRRKKAVQRANGGMWIPPPEMGGPPRGMGRGGGRGGPPPGAGGPGMQRPPPYPGGVGGPQGAGGPLGMRGPPRTSGPPGMRGPPGAVRGRGMGPPLDQMHFGMIPPPTGPPRLPTAFAENLPRHPNKPSATASKDDLPSEEDLEAATVLAETEWAEIYKAFHVFQESLGDEYQPLPIEYMPVQNTPFGAAIYFRTYSIATLQSLYNMALIILHRCHPSMPAVSMVAAGVAAQKTAKLAIDIARITAGLVPTDPTGQINPALGASLIESSMPIFFAAVQYQDQAQREWVVMKLREIARLTGWATASRVLLGCQRAWEKAAEMGRGPAYTRPPFEEEGELFYEAFNEKMAADTGINSMQDEEGSEEAGSKRFLWRTAGRRTAGAVGILGEEGEDLGIGGLNLSMGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCA4</accession>
    <name>D5GCA4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Fructose-1,6-bisphosphatase</fullName>
            <ecNumber evidence="5">3.1.3.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">D-fructose-1,6-bisphosphate 1-phosphohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">GSTUM_00000649001</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>beta-D-fructose 1,6-bisphosphate + H2O = beta-D-fructose 6-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:11064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="EC" id="3.1.3.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="10">Carbohydrate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the FBPase class 1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.11" evidence="5"/>
    <dbReference type="EMBL" id="FN430108">
        <property type="protein sequence ID" value="CAZ82147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837956.1">
        <property type="nucleotide sequence ID" value="XM_002837910.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCA4"/>
    <dbReference type="FunCoup" id="D5GCA4">
        <property type="interactions" value="523"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GCA4"/>
    <dbReference type="GeneID" id="9184825"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000649001"/>
    <dbReference type="eggNOG" id="KOG1458">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039977_1_0_1"/>
    <dbReference type="InParanoid" id="D5GCA4"/>
    <dbReference type="OMA" id="YIPENCP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042132">
        <property type="term" value="F:fructose 1,6-bisphosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030388">
        <property type="term" value="P:fructose 1,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006000">
        <property type="term" value="P:fructose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005986">
        <property type="term" value="P:sucrose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00354">
        <property type="entry name" value="FBPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.540.10:FF:000009">
        <property type="entry name" value="Fructose-1,6-bisphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.80:FF:000001">
        <property type="entry name" value="Fructose-1,6-bisphosphatase class 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.10">
        <property type="entry name" value="Fructose-1,6-Bisphosphatase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01855">
        <property type="entry name" value="FBPase_class1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044015">
        <property type="entry name" value="FBPase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000146">
        <property type="entry name" value="FBPase_class-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033391">
        <property type="entry name" value="FBPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028343">
        <property type="entry name" value="FBPtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020548">
        <property type="entry name" value="Fructose_bisphosphatase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11556">
        <property type="entry name" value="FRUCTOSE-1,6-BISPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11556:SF1">
        <property type="entry name" value="FRUCTOSE-BISPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00316">
        <property type="entry name" value="FBPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18913">
        <property type="entry name" value="FBPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500210">
        <property type="entry name" value="FBPtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000904">
        <property type="entry name" value="FBPtase_SBPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00115">
        <property type="entry name" value="F16BPHPHTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56655">
        <property type="entry name" value="Carbohydrate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00124">
        <property type="entry name" value="FBPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fructose-1-6-bisphosphatase class I N-terminal" evidence="14">
        <location>
            <begin position="21"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="Fructose-1-6-bisphosphatase class 1 C-terminal" evidence="15">
        <location>
            <begin position="213"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00070480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF18913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAZ82147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="352" mass="38273" checksum="517C55C6EF534CAE" modified="2010-06-15" version="1">MTSNGSGSGHHGDSEVKTDIITLTRFLTEEQHRLGPHATGDFTLLCHALQFSFKSIAYYIRRATLINLIGLAGSSNMTGDDQKKLDVIGNEIFVNTMRGCGKVKVLVSEEEEEAIIFPGHGKYAVVCDPIDGSSNLDAGVSVGTIFGIFKLDDDAEGKAKDMLKPGTELVASGFTMYGASANLVLTMKGGSVNGFTLDTALGEFILTHPDMRIPKSKGIYSVNEGNSLYWTEPCKAYFESIKYPQQPGAKPYSARYIGSMVADAYRTLCYGGLFAYPADKKSPKGKLRILYECAPMAMVMENAGGLAITSDQRRMLEVVPEHIHDRSGIFMGSYDEVTKVTEVFKKHGVVAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCD6</accession>
    <name>D5GCD6_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein arginine N-methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00005832001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430109">
        <property type="protein sequence ID" value="CAZ82179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837988.1">
        <property type="nucleotide sequence ID" value="XM_002837942.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCD6"/>
    <dbReference type="FunCoup" id="D5GCD6">
        <property type="interactions" value="976"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GCD6"/>
    <dbReference type="GeneID" id="9184856"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005832001"/>
    <dbReference type="eggNOG" id="KOG0822">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010247_2_1_1"/>
    <dbReference type="InParanoid" id="D5GCD6"/>
    <dbReference type="OMA" id="IKYAWYE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071521">
        <property type="term" value="C:Cdc42 GTPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051286">
        <property type="term" value="C:cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990463">
        <property type="term" value="C:lateral cortical node"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016274">
        <property type="term" value="F:protein-arginine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071470">
        <property type="term" value="P:cellular response to osmotic stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061246">
        <property type="term" value="P:establishment or maintenance of bipolar cell polarity regulating cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903359">
        <property type="term" value="P:lateral cortical node assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903360">
        <property type="term" value="P:protein localization to lateral cortical node"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000100">
        <property type="term" value="P:regulation of establishment or maintenance of bipolar cell polarity regulating cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000149">
        <property type="entry name" value="Protein arginine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.150">
        <property type="entry name" value="Divalent-metal-dependent TIM barrel enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.160.11">
        <property type="entry name" value="Hnrnp arginine n-methyltransferase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025799">
        <property type="entry name" value="Arg_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007857">
        <property type="entry name" value="Arg_MeTrfase_PRMT5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035075">
        <property type="entry name" value="PRMT5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035248">
        <property type="entry name" value="PRMT5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035247">
        <property type="entry name" value="PRMT5_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10738">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10738:SF0">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05185">
        <property type="entry name" value="PRMT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17286">
        <property type="entry name" value="PRMT5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17285">
        <property type="entry name" value="PRMT5_TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015894">
        <property type="entry name" value="Skb1_MeTrfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51678">
        <property type="entry name" value="SAM_MT_PRMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PRMT5 TIM barrel" evidence="10">
        <location>
            <begin position="39"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="PRMT5 arginine-N-methyltransferase" evidence="9">
        <location>
            <begin position="305"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="domain" description="PRMT5 oligomerisation" evidence="11">
        <location>
            <begin position="470"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="5">
        <location>
            <position position="437"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="5">
        <location>
            <position position="446"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="340"/>
            <end position="341"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="394"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="421"/>
            <end position="422"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="site" description="Critical for specifying symmetric addition of methyl groups" evidence="7">
        <location>
            <position position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR015894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR015894-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR015894-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR015894-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ82179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="669" mass="74597" checksum="2AFC8FD91D3CE08E" modified="2010-06-15" version="1">MDAHQPTDPGAPIWYIGNHLPTPDLTISGQTLENEAVQGYDMITTFITNNNFRSRINQVLSNAAAEKRTHIKIDSLDAEEVTVHPGDHISQVIGFASPWLEFDSDDPLVSHVSKQALLYELDYAGFCGIGNVVIPGPRKKENILQFAEVINTALSVSGYAHLPILLPLAENPSSVAEGDKYDEFSAWDTWNTIRTVCKYNSRLSLALQIPAELPTLHAMNRWFAEPIRVILLSSSTFSPNPTGYPALSKLHQSLLTKYTKQRPAPYILLSDQQVATNEKIRYDPLSYLVYLRHLQKNQPPQSIVEKYGSGYQDYLQTPLQPLSDNLESITYEVFEKDPVKYDQYEKAIKLALDARDAASNTVVAVVGAGRGPLVSRALRAAQASGRAIMLFAIEKNPNAYVHLLRHNRDSWNGQVTVIKSDMRSWNPPFVVDILISELLGSFGDNELSPECLDGVQRVLNPSGGISIPVSYSAHFTPIMAPKIHADISSRKNDSDAAETPYVVMLQSFEILAEQEHIHRAWEFTHPLPPNVLSDSAALGGGLIGLGDGGNDHNARKCKATFKVPRRGVMHGLAGYFESVLYGDVELSTRPDTIDMKSKDMISWFPIFFPFKTPLHLPDNCELDVCIWRQTSERKVWYEWVAEAFTRNGGHRYRLGATDLHSSKQNGCLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCR4</accession>
    <name>D5GCR4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00005986001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430120">
        <property type="protein sequence ID" value="CAZ82307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838116.1">
        <property type="nucleotide sequence ID" value="XM_002838070.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCR4"/>
    <dbReference type="STRING" id="656061.D5GCR4"/>
    <dbReference type="GeneID" id="9187926"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005986001"/>
    <dbReference type="eggNOG" id="ENOG502SD64">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_479134_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCR4"/>
    <dbReference type="OMA" id="CNFLEAN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.340">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="304"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="399"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="442"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="569" mass="65356" checksum="22000D7B4EF3EFF3" modified="2010-06-15" version="1">MPENLWITEFNINFITAPSRADAESPPLPNYFPRRRAKFLSMNNVAPARIMAEAAMGFRIIGDLHDRYWTCYVFYDFGNPELKSDARTQVRQGGYGNSPSQRKCLEGFLVCQALDLVLSETKTILDTITKSMGEDKKSQALFNLLLTEDDLRGENYFKTMNKNSVFYPWLLEVCGALQDKCSKSRRVAEQWLSAEKTREYQPRWSEKDQKIFGEEVAQNRADVEARCAMLEKMENNLKERSDWIKTLKESLSSELELREARTSTQLGHTVNLFAVVTTIYLPLTFSTSIVAIQDFNWPSPSKALIKIALPVTFGTLILLMNLAFLRRNLAALKAWAQLSIRRRMAGAPEPKSSNKEPTYQEERPAWKCWEERAHGLREAEKRSTLLTNNDIYDNGSDWWYWYFMAIFIIIVVPVQELTFIIRTLRLQKIKRAGPLKKFVRVPLAPIWVLQLAFVYVIMLAGYAFLSLVGLVHRTAVWLWTGDDIVEDKKDAPYEVEKSFISQLEGDKEILRTKPDCTQKGRGLVGWLMKPAKTMQLLIVVEALRSKKEKEFDAEEQGITVAAMKALHST</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCU0</accession>
    <name>D5GCU0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">D-aminoacyl-tRNA deacylase</fullName>
            <ecNumber evidence="2 6">3.1.1.96</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00000747001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a D-aminoacyl-tRNA + H2O = a tRNA + a D-alpha-amino acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13953"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:10124"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59871"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:79333"/>
            <dbReference type="EC" id="3.1.1.96"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>glycyl-tRNA(Ala) + H2O = tRNA(Ala) + glycine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53744"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:13640"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78522"/>
            <dbReference type="EC" id="3.1.1.96"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the DTD family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.96" evidence="2 6"/>
    <dbReference type="EMBL" id="FN430123">
        <property type="protein sequence ID" value="CAZ82333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838142.1">
        <property type="nucleotide sequence ID" value="XM_002838096.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCU0"/>
    <dbReference type="FunCoup" id="D5GCU0">
        <property type="interactions" value="1480"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GCU0"/>
    <dbReference type="GeneID" id="9183807"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000747001"/>
    <dbReference type="eggNOG" id="KOG3323">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076901_0_1_1"/>
    <dbReference type="InParanoid" id="D5GCU0"/>
    <dbReference type="OMA" id="VFGADMK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051500">
        <property type="term" value="F:D-tyrosyl-tRNA(Tyr) deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106026">
        <property type="term" value="F:Gly-tRNA(Ala) deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.80.10:FF:000001">
        <property type="entry name" value="D-aminoacyl-tRNA deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.80.10">
        <property type="entry name" value="D-tyrosyl-tRNA(Tyr) deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00518">
        <property type="entry name" value="Deacylase_Dtd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003732">
        <property type="entry name" value="Daa-tRNA_deacyls_DTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023509">
        <property type="entry name" value="DTD-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00256">
        <property type="entry name" value="D-aminoacyl-tRNA deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10472:SF5">
        <property type="entry name" value="D-AMINOACYL-TRNA DEACYLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10472">
        <property type="entry name" value="D-TYROSYL-TRNA TYR DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02580">
        <property type="entry name" value="Tyr_Deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69500">
        <property type="entry name" value="DTD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0820">tRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="161"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="167"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ82333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="209" mass="22206" checksum="22094D265561505F" modified="2010-06-15" version="1">MKAIIQRVLSASVTVDKEVVSSIGQGLLVLAAVAPYDTPKDVEAIANKILKLKVWDDEGGGRWKRSVLDLDHQVLLVSQFTLLATTKKGNKPDFHGACPPAVAREIYASLLQKTRELYVEEKKLSPEAGSTKVQDGIFGAMMEVSLVNDGPVTFEISSPMKKVETPLGGGGGEGNTQGGKKSKATSSNEEGQVKLSEAVIDGGELNEGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCZ3</accession>
    <name>D5GCZ3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00006016001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430131">
        <property type="protein sequence ID" value="CAZ82386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="tml:GSTUM_00006016001"/>
    <dbReference type="HOGENOM" id="CLU_3438392_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCZ3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ82386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="10" mass="1275" checksum="61E21A3B05A4072B" modified="2010-06-15" version="1">MRELRSEKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>D5GD01</accession>
    <name>D5GD01_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Sister chromatid cohesion protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00006034001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SCC2/Nipped-B family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430131">
        <property type="protein sequence ID" value="CAZ82394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838203.1">
        <property type="nucleotide sequence ID" value="XM_002838157.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GD01"/>
    <dbReference type="STRING" id="656061.D5GD01"/>
    <dbReference type="GeneID" id="9183857"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006034001"/>
    <dbReference type="eggNOG" id="KOG1020">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_756917_0_0_1"/>
    <dbReference type="InParanoid" id="D5GD01"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090694">
        <property type="term" value="C:Scc2-Scc4 cohesin loading complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061775">
        <property type="term" value="F:cohesin loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140588">
        <property type="term" value="P:chromatin looping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034087">
        <property type="term" value="P:establishment of mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071169">
        <property type="term" value="P:establishment of protein localization to chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990414">
        <property type="term" value="P:replication-born double-strand break repair via sister chromatid exchange"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024986">
        <property type="entry name" value="Nipped-B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033031">
        <property type="entry name" value="Scc2/Nipped-B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21704:SF18">
        <property type="entry name" value="NIPPED-B-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21704">
        <property type="entry name" value="NIPPED-B-LIKE PROTEIN DELANGIN SCC2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12830">
        <property type="entry name" value="Nipped-B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Sister chromatid cohesion C-terminal" evidence="3">
        <location>
            <begin position="20"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="314"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="348"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU364107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ82394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="366" mass="41079" checksum="742667407D0BEA3C" modified="2010-06-15" version="1">MKLRLKIRQPIRGERTLIRTCVPALVALETSANVAIQAISNREHRMLHSKHETIVERGYMDGVLACYIYQRNVIGNGSGATANPFTSKMHPLYEIAKSSRKVRKKLLGNMTMSVDFESPKLYVDPSKPNHLGYSKFVLENLAFFEYGTFDELYQVVTTMERVVSGTGITVAHSIETDILLLKLDSGDTTERIITPDTLKLLATSAAILFMLWECRYFLRKSYSINDNKSRDYKSNKLSAKDANKAPSRAATWSNGAATLWKRFEEIMTDLKSDEGMMKQCKEFVDLLTVDNEFKLAAEGEDPEDLDGERALTPIHDLQEEGGIGGELGTPGNNKKRKFTSGSPEKGLMRPKKKVKAKPWKSAGKDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDB0</accession>
    <name>D5GDB0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Signal peptidase complex subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00006129001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the signal peptidase complex (SPC) which catalyzes the cleavage of N-terminal signal sequences from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum. Dispensable for SPC enzymatic activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SPCS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430142">
        <property type="protein sequence ID" value="CAZ82503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838312.1">
        <property type="nucleotide sequence ID" value="XM_002838266.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDB0"/>
    <dbReference type="STRING" id="656061.D5GDB0"/>
    <dbReference type="GeneID" id="9183967"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006129001"/>
    <dbReference type="eggNOG" id="KOG4112">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134505_2_0_1"/>
    <dbReference type="InParanoid" id="D5GDB0"/>
    <dbReference type="OMA" id="IHLTLWT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005787">
        <property type="term" value="C:signal peptidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045047">
        <property type="term" value="P:protein targeting to ER"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009542">
        <property type="entry name" value="Spc1/SPCS1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13202">
        <property type="entry name" value="MICROSOMAL SIGNAL PEPTIDASE 12 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13202:SF0">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06645">
        <property type="entry name" value="SPC12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ82503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="104" mass="11235" checksum="4D84C1FE9E5BD7AF" modified="2010-06-15" version="1">MDQIQSIIDGFIDFEGQRLAETLTTAILVLAGVLAFIVGYVLSDLKYTVYIGLSGTVIAFLVVVPPWGIYKRNPLTYLPASGVQVQMVDGDADGGKEKEGKKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDD7</accession>
    <name>D5GDD7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00006164001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430142">
        <property type="protein sequence ID" value="CAZ82530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838339.1">
        <property type="nucleotide sequence ID" value="XM_002838293.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDD7"/>
    <dbReference type="GeneID" id="9183993"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006164001"/>
    <dbReference type="HOGENOM" id="CLU_2759638_0_0_1"/>
    <dbReference type="InParanoid" id="D5GDD7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="70" mass="7883" checksum="20434432D3C36633" modified="2010-06-15" version="1">MSVVLYGSRALLGHSWCPSYFLTLFLSSFSDSVWPCYSVFTMFRSFKCFQNKVLPITAPPLSPPGCNPRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDH3</accession>
    <name>D5GDH3_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Mediator of RNA polymerase II transcription subunit 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Mediator complex subunit 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00001020001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the Mediator complex subunit 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430144">
        <property type="protein sequence ID" value="CAZ82566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838375.1">
        <property type="nucleotide sequence ID" value="XM_002838329.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDH3"/>
    <dbReference type="STRING" id="656061.D5GDH3"/>
    <dbReference type="GeneID" id="9184022"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001020001"/>
    <dbReference type="HOGENOM" id="CLU_1267719_0_0_1"/>
    <dbReference type="InParanoid" id="D5GDH3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019680">
        <property type="entry name" value="Mediator_Med1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10744">
        <property type="entry name" value="Med1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0010">Activator</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Mediator complex subunit Med1" evidence="8">
        <location>
            <begin position="11"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF10744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ82566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="218" mass="23237" checksum="EF108C4A5D748EE9" modified="2010-06-15" version="1">MLSSPPGLSVDTLQAKLFPDSPSATGVQRRLYVPTNGMEEEEGIRHEYKLHSIKSILARQIFEIPFSHPKELAGVFSILRQFAHVSALFKSCFSPVAGIIPKTEDSVVAAPLGDKEMDLDAFLADGNDTADGAAQPVPVDISATEGQSGAFTLGVIFPQKTGQGVVGCSFEVGRNADIRVTELSADDGKLGLDANGLEWALAIGSDLGIVVEWLRQRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDI7</accession>
    <name>D5GDI7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00001040001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430145">
        <property type="protein sequence ID" value="CAZ82580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838389.1">
        <property type="nucleotide sequence ID" value="XM_002838343.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDI7"/>
    <dbReference type="STRING" id="656061.D5GDI7"/>
    <dbReference type="GeneID" id="9188173"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001040001"/>
    <dbReference type="eggNOG" id="ENOG502S3EX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019274_0_0_1"/>
    <dbReference type="InParanoid" id="D5GDI7"/>
    <dbReference type="OMA" id="HANGHEP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057678">
        <property type="entry name" value="DUF7918"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36223">
        <property type="entry name" value="BETA-LACTAMASE-TYPE TRANSPEPTIDASE FOLD DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36223:SF5">
        <property type="entry name" value="BETA-LACTAMASE-TYPE TRANSPEPTIDASE FOLD DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25534">
        <property type="entry name" value="DUF7918"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7918" evidence="2">
        <location>
            <begin position="31"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7918" evidence="2">
        <location>
            <begin position="171"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="144"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="220"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="327"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="406"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="153"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="444"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ82580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="514" mass="56726" checksum="CFC4FE437E5A45AD" modified="2010-06-15" version="1">MPTFKHVTLNIHTPQEPPVSTESFSWANTAHSPLPEYGNHRLRNTDKITSYIPSTTNSPFAISITILDSYQPKNQGDERESLAAYVYFDGRQKEETATLLRRGVETWISSRWVEVKEGELAEREFVFKEVGLESFLSGLDLSKSERESNAKNGTRKGVNRRHSMPAKMKAKTDDDDEEDADVGKKESVGQIRVDLFRVRCEGPVRRGVWGGMFKDSRGDEDDEFGGGVDVSHTAGLSDPKPLDRETVSTQSVSHLDPEGVVYASFVFFYRGERQLKKMGLIKPPAPTRADSLSAAPTSKFKKQDFAAMASALESLIAKGSEKNGASKRFNDFRDGNHGVTNGDDLKRKNGKKIPGLDGRDDDVESLVGDELARDERDSLREMMRSEEDVRLEEELIGGVDRIKLKRSHSTSSLRDSGSKDKATTFPPDFSVNNKAKTFATPGKDAIKKSESAHANGHEPETPLKKRRGSGDVDVKSPARGLGFGMQTAPMTGFGDKVEFGGVLAGGMEMEEEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDU9</accession>
    <name>D5GDU9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Store-operated calcium entry-associated regulatory factor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Transmembrane protein 66</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">GSTUM_00006282001</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SARAF family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430153">
        <property type="protein sequence ID" value="CAZ82692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838501.1">
        <property type="nucleotide sequence ID" value="XM_002838455.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDU9"/>
    <dbReference type="STRING" id="656061.D5GDU9"/>
    <dbReference type="GeneID" id="9184487"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006282001"/>
    <dbReference type="eggNOG" id="ENOG502QT6Y">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046802_1_0_1"/>
    <dbReference type="InParanoid" id="D5GDU9"/>
    <dbReference type="OMA" id="WILKGSC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006816">
        <property type="term" value="P:calcium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001256">
        <property type="term" value="P:regulation of store-operated calcium entry"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009567">
        <property type="entry name" value="SARAF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15929">
        <property type="entry name" value="STORE-OPERATED CALCIUM ENTRY-ASSOCIATED REGULATORY FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15929:SF0">
        <property type="entry name" value="STORE-OPERATED CALCIUM ENTRY-ASSOCIATED REGULATORY FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06682">
        <property type="entry name" value="SARAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 16" id="KW-0732">Signal</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072300" description="Store-operated calcium entry-associated regulatory factor" evidence="16">
        <location>
            <begin position="19"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="156"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="181"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="245"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="190"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="203"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="251"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="265"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="292"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAZ82692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="303" mass="32583" checksum="5432928A5A2853F4" modified="2010-06-15" version="1">MKLPTVTIFLLATSAAAAKLKSGQKILLSEVPALTLRSDQMTKNRRVPAVPQLKCVGGEGKGKYEVDVMRCKNIGSDYNDEYVSWSCTADLPREFKLGSTDVICEGYDSADDQYVLKGSCGVEYRLALTDYGYEKFGYGGSVFKRLPSKGIEAETFLFWTFFFGIVGWILYSVYKNRNNSIPRAREPRRPGGGGGWFGGGGGDPPPPYDPRPPPKPAFPSSSSQQWRPGFWSGLAAGAAGSYLMGNRERANQTQTAPQWFQSSGGPSGGYGGPSWGGGGGGGESSSAGAMRHSSTGYGQTRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDZ2</accession>
    <name>D5GDZ2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00001110001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430155">
        <property type="protein sequence ID" value="CAZ82735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838544.1">
        <property type="nucleotide sequence ID" value="XM_002838498.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDZ2"/>
    <dbReference type="GeneID" id="9184530"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001110001"/>
    <dbReference type="HOGENOM" id="CLU_3070385_0_0_1"/>
    <dbReference type="InParanoid" id="D5GDZ2"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="53" mass="6432" checksum="75C0D7EE04F477F7" modified="2010-06-15" version="1">MQKVRSYYLFKFVFELLIKLLFHIIPSRAFFSLRFLLNFSKSSCLFSLVIYCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEE6</accession>
    <name>D5GEE6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00006445001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic RPA49/POLR1E RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430175">
        <property type="protein sequence ID" value="CAZ82889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838698.1">
        <property type="nucleotide sequence ID" value="XM_002838652.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEE6"/>
    <dbReference type="FunCoup" id="D5GEE6">
        <property type="interactions" value="630"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GEE6"/>
    <dbReference type="GeneID" id="9187257"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006445001"/>
    <dbReference type="eggNOG" id="KOG4183">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034953_2_1_1"/>
    <dbReference type="InParanoid" id="D5GEE6"/>
    <dbReference type="OMA" id="LWNHYVA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009668">
        <property type="entry name" value="RNA_pol-assoc_fac_A49-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14440">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06870">
        <property type="entry name" value="RNA_pol_I_A49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ82889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="434" mass="49435" checksum="E791C426D5C69A0B" modified="2010-06-15" version="1">MPTETKRKRESSSKINGPLPDYTITVAPTSDYPPILASSHGISMPPTTQFTPYAHTANPPAKKSRKAEEFLLYGETPRIGYEGREMSEDGEYRRHYVGIFNPETNGIELHVAPKLHFSRMIKAHIKRDRLVDGKGVVMSNVDSRTALGKEFGTKKSQKRLRELELNKIDVSDMGKDITTSIVSQVEANTKDMPTATELGNQIRENKPIPKVNHDAKHPNEVYNLEDIIGSDEYNVIWVREWERGEELRSKSSFVQSRIRKLSEDKNKKHTRPLKILRYISFMIDFYLFQQRGRGRLPAMDRVKKALSIDHVIAEGFFRRFTEKTISGEGGSDKETYAVSPALANKILYHMAVLCLMVDQYDVDIFELKNDLGVQPKELTLAFKEVGCSIRELTKPQYTAMKMTKAEAAQHKRAVLKIPLEFPKPPTRRAKATGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFX0</accession>
    <name>D5GFX0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00001917001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430221">
        <property type="protein sequence ID" value="CAZ83413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839222.1">
        <property type="nucleotide sequence ID" value="XM_002839176.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFX0"/>
    <dbReference type="FunCoup" id="D5GFX0">
        <property type="interactions" value="796"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GFX0"/>
    <dbReference type="GeneID" id="9181759"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001917001"/>
    <dbReference type="eggNOG" id="KOG0028">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061288_18_1_1"/>
    <dbReference type="InParanoid" id="D5GFX0"/>
    <dbReference type="OMA" id="NHLPFGA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000003">
        <property type="entry name" value="Calmodulin A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050145">
        <property type="entry name" value="Centrin_CML-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23050">
        <property type="entry name" value="CALCIUM BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00036">
        <property type="entry name" value="EF-hand_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="94"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="221"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="35"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="45"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ83413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="253" mass="27801" checksum="7E1DE11125595BC6" modified="2010-06-15" version="1">MFSAGPNNPRQGTAPPAGGRNRRAQGLRQSPPPTASASTNPLGGSVLGGVLFGNTGGGRGGTGHQHHQRERDRDLTSQQQRPQERGTVVELSEEQRAEINEAFHLFDLDKDRMIDYHELKVAMKALGFDVPKPELLQILREHGTPGPGQQAQPGAQPSRLYISQEAFTSIMTQKILERDPLDEILRAFDLFANAAGGGTGQEPRIGIEDLRRVARELGETLEEEELRAMIEEFDIDNDGMISKEEFIAICRGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5GG47</accession>
    <name>D5GG47_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00007177001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNU66/SART1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430242">
        <property type="protein sequence ID" value="CAZ83490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839299.1">
        <property type="nucleotide sequence ID" value="XM_002839253.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GG47"/>
    <dbReference type="FunCoup" id="D5GG47">
        <property type="interactions" value="780"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GG47"/>
    <dbReference type="GeneID" id="9184552"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007177001"/>
    <dbReference type="eggNOG" id="KOG2217">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009379_2_0_1"/>
    <dbReference type="InParanoid" id="D5GG47"/>
    <dbReference type="OMA" id="KRRDYTG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000481">
        <property type="term" value="P:maturation of 5S rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045347">
        <property type="entry name" value="HIND"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005011">
        <property type="entry name" value="SNU66/SART1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14152">
        <property type="entry name" value="SQUAMOUS CELL CARCINOMA ANTIGEN RECOGNISED BY CYTOTOXIC T LYMPHOCYTES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14152:SF5">
        <property type="entry name" value="U4_U6.U5 TRI-SNRNP-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19252">
        <property type="entry name" value="HIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03343">
        <property type="entry name" value="SART-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="18"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="267"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="523"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="623"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="107"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="281"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="362"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="436"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="452"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="465"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="632"/>
            <end position="643"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ83490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="658" mass="74119" checksum="45CDB6519150480C" modified="2010-06-15" version="1">MEESLSIEETNKIRLSLGLKPLRVDTDAPATSGKPEDSVPDNSVEAQERRAVDNWKRHQDDIDKEIARKARIEGIKKARDAEKRFAKLEGKGLGEADENEEDTTTWVRKMKKRQKKLAERMAREMEEELQRAAAERSEYTAEDLTGVRVGHDLGELDGEGMVLTLKDTTIEENEEEGDELISTDLAERERLKERLDLKKKKPTYNAYEDESEDGEKRILAQYDEEIDGKKKKRFVLDGTGSASNVDAHRREVAEKLKSIAVTLDPIKSEASSDYVDPATLKIRKPKKMKARATRKRSEDDDDTLAPPPEAPMEDSMEVDSGSAPAQPKPKRVFEETSFVDDDDLQSALAAQRRTALKKRKMLKPDDLARQLREESAAAATPGADSPIREDDGEGEPGLVIDETSEFVATLRAPVVPERRSRSGAPAHSPPATSMAEGEDEDEDEDGPEEMDVDIKPEMKPDSETGEISSTGLAQETTITHGVGATLAMLHQRGLLTRDSESDVKINLQRDREKFRTAKRLRELEAEEKAKSQRLRDRQSGKFDRMSAREREEHARWENKQRDLQEAKEMAARFKEYKPDVKLSYKDEFGREMSQKEAFKHLSHQFHGKGSGKAKTEKRLKKIEDEKKREAASSLNASATENAVQSAAKKSKQAGVRLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5GG82</accession>
    <name>D5GG82_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00001996001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430246">
        <property type="protein sequence ID" value="CAZ83525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839334.1">
        <property type="nucleotide sequence ID" value="XM_002839288.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GG82"/>
    <dbReference type="GeneID" id="9184594"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001996001"/>
    <dbReference type="HOGENOM" id="CLU_2172910_0_0_1"/>
    <dbReference type="InParanoid" id="D5GG82"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ83525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="110" mass="12212" checksum="26C966B6788228F3" modified="2010-06-15" version="1">MGLVWSGQAGERAHEVFEQFWWRLRRRLSHVGAEPRLFRVGGALRRVVASNGFGGWVRGPPPLGGGAKLFCFFCCPIVGEPTLGWRRVCEAVSRRFCLCGIARVLVGCSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGQ2</accession>
    <name>D5GGQ2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00007463001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430275">
        <property type="protein sequence ID" value="CAZ83674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839483.1">
        <property type="nucleotide sequence ID" value="XM_002839437.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGQ2"/>
    <dbReference type="FunCoup" id="D5GGQ2">
        <property type="interactions" value="80"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GGQ2"/>
    <dbReference type="GeneID" id="9185596"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007463001"/>
    <dbReference type="eggNOG" id="KOG4197">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_416844_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGQ2"/>
    <dbReference type="OMA" id="ESSAIWA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057027">
        <property type="entry name" value="TPR_mt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47938:SF35">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN 4, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47938">
        <property type="entry name" value="RESPIRATORY COMPLEX I CHAPERONE (CIA84), PUTATIVE (AFU_ORTHOLOGUE AFUA_2G06020)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13812">
        <property type="entry name" value="PPR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23276">
        <property type="entry name" value="TPR_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="166"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="224"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Pentatricopeptide repeat-containing protein-mitochondrial" evidence="4">
        <location>
            <begin position="291"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="296"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="332"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="530"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="552"/>
            <end position="565"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ83674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="730" mass="82177" checksum="34712611FA6A41D7" modified="2010-06-15" version="1">MSCQSRALASHARHGRGTRASGFLGMIKRLEAHVQLIEKRAADIEAQGAARKRLPDLEMTEELQDQAYEALMTPGSKTAVFLPQPETVSKMLPASIVERLGSAAALVDTSTPLWGTVIGQLEFNGGFQGMEIVDVNCLLVSMGLESRVENSLKLQEMMEKAKLEPDTLTYDLLMMAHAQLGCPEEVKTLFESLKKSKGLVPGPCTDLSIILTDTMLSIEGVPPTIFTYAHLLKAFSLQADIEGAGKAFTEMREAGIRPNIVVYTTLIKTCLNRREFPTAWRIFDLMKLKSKATAPDTRTFALMLHACAMEGESEKALNLFKDMTDRMRLKPTKETYHALIHALAVRKDYYQEVWRYATEMQKEGFKMDRRVLGTLIQACGKRGDLTRARMLIRHMLGSGKEDIVPDSTTYQCLFRAYANTGGKKKCVEFVEGGSPETSAPYVGPDTRLVKQEESFKEDAIPFSQNTILNTRLEIMREARRVMEWLCTVKPHLVDTQLMNALLDAYCNHYYPFKFKVLYYTAFTDPKESDYLEPTTSGTGAGNLTPEPNGVPAADTIDTGKGTGTDAEPDKNTHPNSPDQSALRRIVPRNLYTFQTALSGAYKHYDLSFAWDIYNDRIKYLLTKQYYSLYREAERRRLDAKAECMMINILARCEMLGEAAARLKTTCKKYKIYESDIAILHIKALQLDDKQTLKVIKECVGTTRYRDYAVGARHKLPWKGEGEAEDSSRTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGR9</accession>
    <name>D5GGR9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00007487001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
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        <property type="protein sequence ID" value="CAZ83691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002839454.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGR9"/>
    <dbReference type="STRING" id="656061.D5GGR9"/>
    <dbReference type="GeneID" id="9185613"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007487001"/>
    <dbReference type="eggNOG" id="KOG0068">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019796_1_1_1"/>
    <dbReference type="InParanoid" id="D5GGR9"/>
    <dbReference type="OMA" id="VIVTAHQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12183">
        <property type="entry name" value="LDH_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058205">
        <property type="entry name" value="D-LDH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43026">
        <property type="entry name" value="2-HYDROXYACID DEHYDROGENASE HOMOLOG 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43026:SF1">
        <property type="entry name" value="2-HYDROXYACID DEHYDROGENASE HOMOLOG 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00065">
        <property type="entry name" value="D_2_HYDROXYACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00670">
        <property type="entry name" value="D_2_HYDROXYACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase catalytic" evidence="5">
        <location>
            <begin position="36"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="6">
        <location>
            <begin position="137"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ83691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="360" mass="39211" checksum="797462E8D48B7735" modified="2010-06-15" version="1">MKVAFYSSKKVSPASFLLATLFIDSTSHRLGLAPSTQYDIDSFGDANSNTGIELNFLESRLEKSTVPLAAGHDAVCIFVNDYCDADVLEGLANLGVKCVALRCAGFNNVDLQAAERLGIEISRVPAYSPEAVAEFAVGMIMTVVRKYHKAYNRVREGNFLLDGLLGFNLFQKTIGIIGTGKIGLLTGKILAKGFGCNVIAYDLYPGKQADEYGIRYVNTLEELLRQSDIISLHCPLTAGTKYMINSTTIGQTKRGVILINTSRGELIDTAALINALKSGHLGAVGLDVYEKESAYFFADSSAKIIYDDNFARLLSFYNVFVSGHQAFLTVEALKNIADTTLQNLQDRANGKKSPNLVERK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGW5</accession>
    <name>D5GGW5_TUBMM</name>
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            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00002069001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="CAZ83758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839567.1">
        <property type="nucleotide sequence ID" value="XM_002839521.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGW5"/>
    <dbReference type="GeneID" id="9181729"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002069001"/>
    <dbReference type="HOGENOM" id="CLU_3368797_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGW5"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ83758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="35" mass="4276" checksum="2C836FF7C03BF653" modified="2010-06-15" version="1">MSVKDHSKIEELIKDFLKQLYHKLIVHIRCIKIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>D5GH46</accession>
    <name>D5GH46_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007699001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430297">
        <property type="protein sequence ID" value="CAZ83839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839648.1">
        <property type="nucleotide sequence ID" value="XM_002839602.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GH46"/>
    <dbReference type="STRING" id="656061.D5GH46"/>
    <dbReference type="GeneID" id="9185093"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007699001"/>
    <dbReference type="eggNOG" id="ENOG502SESI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1143260_0_0_1"/>
    <dbReference type="InParanoid" id="D5GH46"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="162"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="243" mass="26862" checksum="E790F563127A9F34" modified="2010-06-15" version="1">MASPPPAPPPDETILSTSSIFFSFSPAPLPGIFSFTSPPPAATTVSSSYSSSSYPRKRARPLADIDGLDGDFRDYNKKRRLRLRLITSRLSEPFAVPPTNILDRGNCSRVALWAKTRAVSRNVLRKAAILNKIKRDAMAARAAQERQVEIARQQFREKIAAAAQSSPKRNHVAQFHPPLDQGACIVKPRKTKGSKSSQHSKKNHKSDLPSIPDPYGIPKHRRPPTPPSESELDWQRRGMGLGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>D5GH64</accession>
    <name>D5GH64_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007766001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430308">
        <property type="protein sequence ID" value="CAZ83889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839698.1">
        <property type="nucleotide sequence ID" value="XM_002839652.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GH64"/>
    <dbReference type="STRING" id="656061.D5GH64"/>
    <dbReference type="GeneID" id="9182594"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007766001"/>
    <dbReference type="eggNOG" id="KOG4253">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044758_1_0_1"/>
    <dbReference type="InParanoid" id="D5GH64"/>
    <dbReference type="OMA" id="YFYYALP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39470:SF1">
        <property type="entry name" value="CHORISMATE SYNTHASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39470">
        <property type="entry name" value="CHROMOSOME 10, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="136"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="213"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="361" mass="40054" checksum="1021C83B78046387" modified="2010-06-15" version="1">MVSWSTIQPFLILLGPIIYSRGKAFYQSTKLRGPPRVLTPGTHRILNILFATGVVALLSTLPAFAPENIFQKTGSRLQIPTDVLFNRLSKIRDITPDDEILRARLVPKEARLAYATFGPKPLVECSWCSLEEANTYLFYALPTLALPHLLHIAVLGVVTSSYFSPFGSVWRTQATVAGLALFLAELCVVGVSTSDHNANALAKLPADVVWTHWRLILWRGIGIAFVDAWLGYVIFLTGTGRWTAGLDGLRVQEKLETLNKASELVYAKLHSENLLRQTVLRNKDLREKSFELWMAEEKVGKEIMLDEEVKNVRANLTLDVTQLTKGAAQKSEGMLNTISTWRGLPAQVPPTSFPQPSTTAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>D5GH97</accession>
    <name>D5GH97_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007809001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430308">
        <property type="protein sequence ID" value="CAZ83922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839731.1">
        <property type="nucleotide sequence ID" value="XM_002839685.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GH97"/>
    <dbReference type="GeneID" id="9187869"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007809001"/>
    <dbReference type="HOGENOM" id="CLU_2164548_0_0_1"/>
    <dbReference type="InParanoid" id="D5GH97"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="111" mass="12511" checksum="85CA0147BD7E48D3" modified="2010-06-15" version="1" fragment="single">SRGSGCLFRKCAWWKGIICSGSGFFHVQFLHCVLSLPFFFFSCLFSLSSTPITREIFSRVFHSSLFIAPPSMIDGVLDFFPFIWSLWLCFSIAMAIAAVAFHTHTHTSSES</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHB2</accession>
    <name>D5GHB2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00007731001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430308">
        <property type="protein sequence ID" value="CAZ83864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839673.1">
        <property type="nucleotide sequence ID" value="XM_002839627.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHB2"/>
    <dbReference type="STRING" id="656061.D5GHB2"/>
    <dbReference type="GeneID" id="9182569"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007731001"/>
    <dbReference type="eggNOG" id="ENOG502S5WH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094649_1_0_1"/>
    <dbReference type="InParanoid" id="D5GHB2"/>
    <dbReference type="OMA" id="SFWWLRS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41390">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41390:SF1">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 213 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="89"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="176"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ83864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="211" mass="22885" checksum="0F4332A894A9FAF8" modified="2010-06-15" version="1">MQNSSRTEPPEPILFPALKAGAVAGLAGTLAGGVIGILRSTPHAKTFAVLTGANTFVLGWTYWAVRSTMCRAIYDASPPGTAMVPRDRVLISGASGAIAGGFLGIILRKMPNVVPGAIVWGLVGLGGQYAYNVADARHTQEVIEKMENQEPKESILDRAIRSRWSPVKRLTSKEYADMLKEKLLAVEADIAIANEEIEKLERQKREPMEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHF8</accession>
    <name>D5GHF8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00007844001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430318">
        <property type="protein sequence ID" value="CAZ83951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839760.1">
        <property type="nucleotide sequence ID" value="XM_002839714.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHF8"/>
    <dbReference type="STRING" id="656061.D5GHF8"/>
    <dbReference type="GeneID" id="9183626"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007844001"/>
    <dbReference type="eggNOG" id="KOG0725">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_1_1"/>
    <dbReference type="InParanoid" id="D5GHF8"/>
    <dbReference type="OMA" id="SWKDEIM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760:SF115">
        <property type="entry name" value="3-OXOACYL-[ACYL-CARRIER-PROTEIN] REDUCTASE FABG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASES/REDUCTASES FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ83951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="252" mass="27038" checksum="504FBE13B22955F9" modified="2010-06-15" version="1">MATPSKANPIFPLPSPNSITQLTIPSLAEAGADIAVFDIIPPTPGFLSISERFSVRAVHYLADVASPDSLAAAFESFKKDFGGKLDICVPCAGVNKNVEFLDTTWEDHERLLGVNVMGVYHTAQLAAKTMIEYGTKVGSIVVIASIASYMAIRSQNSSAYCGTKGAVRAMVPGIAAEMVKYGIRVNSLSPGYVRTEMTAPFPHLLESWKDEIMNHRVAEPEDIRGACVFLASDASAYMTGQDLCIDGGVTKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHF9</accession>
    <name>D5GHF9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007845001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430318">
        <property type="protein sequence ID" value="CAZ83952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839761.1">
        <property type="nucleotide sequence ID" value="XM_002839715.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHF9"/>
    <dbReference type="GeneID" id="9183597"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007845001"/>
    <dbReference type="eggNOG" id="ENOG502QU9T">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029940_1_0_1"/>
    <dbReference type="InParanoid" id="D5GHF9"/>
    <dbReference type="OMA" id="WWEFSNI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10938">
        <property type="entry name" value="CE4_HpPgdA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.370">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002509">
        <property type="entry name" value="NODB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037950">
        <property type="entry name" value="PgdA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47561">
        <property type="entry name" value="POLYSACCHARIDE DEACETYLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_6G05030)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47561:SF1">
        <property type="entry name" value="POLYSACCHARIDE DEACETYLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_6G05030)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51677">
        <property type="entry name" value="NODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NodB homology" evidence="1">
        <location>
            <begin position="118"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="414" mass="47758" checksum="4893BD844D66E464" modified="2010-06-15" version="1">MGFDCAIYSYSTVQVPLRGALIHPTLAEAGQILIDNRFPPPPKLIILLHSGPFPLSPSFIYWPSLPPFGVIPLKVPPYRPSNTCQKIIEMGKKRVLCTYGIDIDAVAGWLGSYGGEDSTSDISRGMFAGTIGVRRLLKLFEKYNMKTTWFIPGHSLETFPEECAMVRDAGHEIGLHGYSHENPVNMTWEQQRDVLDKSYRLLTDFCGKPPRGNVVPWWECSEEGTELMLKYGIEYDHSMSHHDCQPYYLRRGDKWTKIDYSKKAEEWMKPLEKGQESGLVEIPGSWYIDDLPPMMFIKNNANSHGWVNPRDIEDIWRDHFDYFYREYDEFIFPITIHPDVSGRPHVLLMHERLIEHINKHEGVEWVTMEYICDDFKKKNPYPAGALLPSPPGEILTKSKEQVEKEIEYKGDEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHG4</accession>
    <name>D5GHG4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00007855001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430318">
        <property type="protein sequence ID" value="CAZ83957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839766.1">
        <property type="nucleotide sequence ID" value="XM_002839720.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHG4"/>
    <dbReference type="STRING" id="656061.D5GHG4"/>
    <dbReference type="GeneID" id="9183602"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007855001"/>
    <dbReference type="eggNOG" id="ENOG502RYAY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010112_0_0_1"/>
    <dbReference type="InParanoid" id="D5GHG4"/>
    <dbReference type="OMA" id="FMYNAVL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046623">
        <property type="entry name" value="DUF6536"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35395">
        <property type="entry name" value="DUF6536 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35395:SF1">
        <property type="entry name" value="DUF6536 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20163">
        <property type="entry name" value="DUF6536"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="174"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="388"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="479"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="519"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="545"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="589"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="631"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="domain" description="DUF6536" evidence="2">
        <location>
            <begin position="61"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ83957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="743" mass="82114" checksum="E5980AC66A5D4B7F" modified="2010-06-15" version="1">MLNPKKKFRKWTAISPGDDHHHREGGSRSSSIELLALGSETTREWSTSLSSVWKPVWYTGWHTGVLGCAASVVLVLFINTGLTIYAATNPEYKMEGGVGTLYLGSCDKSRTIGMWLHLGINALSTLLLSGSNYTQQCLAAPTRSEVDAAHARRLWMDIGVPSVRNLFRIKWERTLLWIAIGVTSIPLHLLYNSAVYNSIAANDYVVTLVTDNYIEQAPQSNATEALLDFYQELYNIENRTTMNFPMYGGVAETFNGVLEGYNTGAQAYEDLTPGGCTKLYNTDFISSHRNLFLITGQGSNVTSNNTIINIMEVSAEGISPSSWMCDYEEATEGGSYLRTGRTCISNNLVSNVTGGAPWRVQLSTGSEVEISGCKSEKISELEKCKVQFSLGIMIVVICCNFVKAGCMVMAVVRSREPTLVTLGDAVDSFLRNPDPTTEGICFADRQFAKQQWRHGGRTGPRQWKQEGVQRWWTSVSKTRWITCNFFFLITIMVSGVLLRMGIRNDGKYFDIDLKGFGKVSSGSILNIYFGNITQAILLANLPQTILSFLYLTYNSVFTCMLSGHEWSLFSHHHRTLRVTSPRPGQRSTYWLQIPYTYAIPLMTLSGLLHWLTSQSIFLARVEIWDPLGRGIVGTISTVGYSCIAIISVLTLGIIALLIAAGMGYRQFSAETATVGSCSVAISAACHAWEEPDVIIGKKVRWGDVGIVQDLGFRHLTFSSEEGVGKPIFGEVYAGKREGRSDSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHI9</accession>
    <name>D5GHI9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00007937001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430319">
        <property type="protein sequence ID" value="CAZ83982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839791.1">
        <property type="nucleotide sequence ID" value="XM_002839745.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHI9"/>
    <dbReference type="STRING" id="656061.D5GHI9"/>
    <dbReference type="GeneID" id="9183636"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007937001"/>
    <dbReference type="eggNOG" id="KOG0423">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030988_0_1_1"/>
    <dbReference type="InParanoid" id="D5GHI9"/>
    <dbReference type="OMA" id="VLITIRC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23804">
        <property type="entry name" value="UBCc_UBE2S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="6">
        <location>
            <begin position="10"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="166"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="166"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="207"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="267"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="280"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="3">
        <location>
            <position position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ83982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="302" mass="33261" checksum="EFAFF8BCDB143C8E" modified="2010-06-15" version="1">MATNQHHQSSHRRRLAQDHSTLRTTPPVDYFFPPNTTSDDLTSLQIFLAGPTQTPFESGLFQIHLRIPPTYPAEPPKASFKTKIFHPNVDERSGDVCVDTLKRDWKPTLTLRDVLITIRCLLVYPNPTSSLNEAAGKLLLEDYEGYARHARLMTDVHAGVPEELKGLVEESRRRGEDEDQGVGKKDPRAPGEQKRKIKQAVKPSAAVRKDKGKEKEKEKCSDSEGGDSGKENAETRRSVARPSPKFKQGQSAGSRLPLTAAAATTATKKELVTKKDPSKKSAASGKSSKTGAAARKVGLKRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHQ0</accession>
    <name>D5GHQ0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD repeat-containing protein JIP5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">WD repeat-containing protein jip5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00007988001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat WDR55 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430320">
        <property type="protein sequence ID" value="CAZ84011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839820.1">
        <property type="nucleotide sequence ID" value="XM_002839774.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5GHQ0">
        <property type="interactions" value="96"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GHQ0"/>
    <dbReference type="GeneID" id="9184173"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007988001"/>
    <dbReference type="eggNOG" id="KOG2444">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_471876_0_0_1"/>
    <dbReference type="InParanoid" id="D5GHQ0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050505">
        <property type="entry name" value="WDR55/POC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44019">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44019:SF20">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00039238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ84011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="578" mass="63957" checksum="3683DCC49620C39A" modified="2010-06-15" version="1">MVYKCNKIFLSSDIFSLSVHPTMPLYAAGLLSGRLEVYRWGDMSEESGEPMGVMVDYGGKYAMQWGTRRYRESCRAVAFTTGGECIFSAGVNSLLKLADVGTGQVVSKAYIPSLKNLTHEYPSVITPLSPVHLLVGTDSGRIHLYDVRENSLTRPAQTWKKVHDDYISSITPLPSYGGGLPRQFVSTGASTLAYIDFRRQGKAVIRSEDQEDEQLCSAYVSGLPARAGRGGEKILVGNSSGVVALWNRDEWEDQRDRINFSKPSGESVDAVVALPDTFQHRNSEFGRFVAAGSADGKVRIAKLGSNRIVETLQHSLTIEKVKGTLATPSLSNGDESVDGVTELGIDCEGNLVSAAGSTIKVWYWDENTPDVSAPREKGKRSPNEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPAIALWFYRFQKPTLTPTWRMGLAIMSNNFPRSAEKSERAGLEKLRARVRRNLLIFVDWIKRLCVMLRTFPFSFGVGLDIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHQ7</accession>
    <name>D5GHQ7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00007996001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430320">
        <property type="protein sequence ID" value="CAZ84018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839827.1">
        <property type="nucleotide sequence ID" value="XM_002839781.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHQ7"/>
    <dbReference type="STRING" id="656061.D5GHQ7"/>
    <dbReference type="GeneID" id="9183672"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007996001"/>
    <dbReference type="eggNOG" id="KOG1644">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061027_1_0_1"/>
    <dbReference type="InParanoid" id="D5GHQ7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030620">
        <property type="term" value="F:U2 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044640">
        <property type="entry name" value="RU2A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10552">
        <property type="entry name" value="U2 SMALL NUCLEAR RIBONUCLEOPROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10552:SF6">
        <property type="entry name" value="U2 SMALL NUCLEAR RIBONUCLEOPROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="41"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="150"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="77"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ84018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="184" mass="20758" checksum="51FD17FBC66CD910" modified="2010-06-15" version="1">MRLTTELLQAAHSYLNPLNDRELDLRGHKIPAIENLGVAATRRHRLDGQRHPSPRKLPPKRTPENPLTRAQPDIHHPAHPRRQPAKPNHYRKVKDVEREAAGRLFGSVSEPSALAARIMGIKSRTFDISEPLNGGQKEYTVRLSAAERKSIEARIRAAKSLEEVARLERELREGLQSSGDAMEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHZ9</accession>
    <name>D5GHZ9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Plasma membrane fusion protein PRM1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00008211001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Involved in cell fusion during mating by stabilizing the plasma membrane fusion event.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Cell membrane</location>
            <topology evidence="2 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the PRM1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="FN430322">
        <property type="protein sequence ID" value="CAZ84142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839951.1">
        <property type="nucleotide sequence ID" value="XM_002839905.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHZ9"/>
    <dbReference type="FunCoup" id="D5GHZ9">
        <property type="interactions" value="18"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GHZ9"/>
    <dbReference type="GeneID" id="9184780"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008211001"/>
    <dbReference type="eggNOG" id="ENOG502QRP5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010191_1_0_1"/>
    <dbReference type="InParanoid" id="D5GHZ9"/>
    <dbReference type="OMA" id="NVFGWVN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032220">
        <property type="term" value="P:plasma membrane fusion involved in cytogamy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026777">
        <property type="entry name" value="PRM1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31030">
        <property type="entry name" value="PLASMA MEMBRANE FUSION PROTEIN PRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31030:SF1">
        <property type="entry name" value="PLASMA MEMBRANE FUSION PROTEIN PRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 10" id="KW-0184">Conjugation</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="48"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="319"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="405"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="603"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="638"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="678"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="724"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="651"/>
            <end position="662"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ84142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="746" mass="80755" checksum="29C6DF5CFE89438D" modified="2010-06-15" version="1">MDNNNNSHMFTTDAPSYEDAMNASRRAPPTTHELTPYLGLRARLSQVWFNRWTLLLFLVLVRVILATQSLDGDLRSARREALSACTGVESMGSAMASMPHYMSKGVNEVAAMGIETAVKALEKTLLLLITGVQEIVVFVVNMITSTYVCLITLAIQGSLGAVLEATEKIADFVNNTMGKIFDDVGRDVKSVQDGVNKVSKALEKVPNFFGSDISFPEVKLPSLDQLKKVQIPTSFDDDLAKLKDSIPTFDEVQKAAHDAIRIPFQLISKKVNESIGVYEFDRSVFPVPEKERLTFCSDNPKINNFFDGLEGTVDKVKKILIGVIICLAILALIPMAYREWWNWRTTQSRAFMLHTAPTYDPVDVIQIASRPYSSSVGLKLAGLFKSSRRQTLVRWAVAYATSTPALFVLSLGIAGLFGVLAQYILLKQVESAAPALAAEVGEFADLVVKELTAASMSWAVKTNSAINSTNSDINKELFGWVVNGTDSLNDTLTVFVDKMYEGVDKFFGKTPLAKPIKDVLNCLIGIKVKGIQKGLTWANENAHINLPLLPNDTFSLGAADSIAPEASGAASFLSDTSSQASDMITDVVIKLTNKWEKMLTEEAIISACIVGIWILVLIIALVRTIALFYTSDKHRGEAGGGLPLTPVTQQRSAPSRGANNNNGSGGFITSPVFPAFGGSSNSSTGGGSPAGGESMSPADGYYEPDEKADPNARWLQVGGSVAVENSQGEYRRSVHASVHPVYPGKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5GI97</accession>
    <name>D5GI97_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ferric-chelate reductase (NADPH)</fullName>
            <ecNumber evidence="3">1.16.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">GSTUM_00008352001</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>2 a Fe(II)-siderophore + NADP(+) + H(+) = 2 a Fe(III)-siderophore + NADPH</text>
            <dbReference type="Rhea" id="RHEA:28795"/>
            <dbReference type="Rhea" id="RHEA-COMP:11342"/>
            <dbReference type="Rhea" id="RHEA-COMP:11344"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.16.1.9"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ferric reductase (FRE) family.</text>
    </comment>
    <dbReference type="EC" id="1.16.1.9" evidence="3"/>
    <dbReference type="EMBL" id="FN430324">
        <property type="protein sequence ID" value="CAZ84240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840049.1">
        <property type="nucleotide sequence ID" value="XM_002840003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GI97"/>
    <dbReference type="FunCoup" id="D5GI97">
        <property type="interactions" value="332"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GI97"/>
    <dbReference type="GeneID" id="9181958"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008352001"/>
    <dbReference type="eggNOG" id="KOG0039">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010365_3_1_1"/>
    <dbReference type="InParanoid" id="D5GI97"/>
    <dbReference type="OMA" id="GKNGWVF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052851">
        <property type="term" value="F:ferric-chelate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015677">
        <property type="term" value="P:copper ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06186">
        <property type="entry name" value="NOX_Duox_like_FAD_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013112">
        <property type="entry name" value="FAD-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013121">
        <property type="entry name" value="Fe_red_NAD-bd_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051410">
        <property type="entry name" value="Ferric/Cupric_Reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361">
        <property type="entry name" value="FERRIC/CUPRIC REDUCTASE TRANSMEMBRANE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361:SF24">
        <property type="entry name" value="REDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G10820)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08022">
        <property type="entry name" value="FAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08030">
        <property type="entry name" value="NAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00052">
        <property type="entry name" value="Ferric_Reductase_Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01168">
        <property type="entry name" value="Ferric_reductase_subgroup_(FRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072090" description="ferric-chelate reductase (NADPH)" evidence="14">
        <location>
            <begin position="25"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="137"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="170"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="209"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="248"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="277"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="15">
        <location>
            <begin position="329"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAZ84240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="608" mass="68319" checksum="695391F5B54DAFB6" modified="2010-06-15" version="1">MAVMAMLGSTTMLAWVLFGGLTFATQPVALTPAQIAANAAGKRHNSDFSKWMIISLAAFAALPLFYRFCSVIYSRIRLLVSLGSENQRFFAAPPSTWVSFIKKHVIYSPLFRVRHNREFRLSTAVSVGTLPTRFQTFFLLGYLAGNIVFCTVMIDRAVDTMSMLGELRNRTGVLAAVNMVPLFLLAGRNNPLIQLMGISFDSYNLIHRWLGRIVVLQAVAHTSCWVAAKVMKSGWEAVVTPINTNPFIRFGFISVVCFVFLMLHSPSAIRHAFYETFLVLHIMAAALGVAGLYYHLEIKGFAGLKHVKIAITLWAFDRGLRLLRILYRNVGRKMTTATCEALPGDAVRVTLHLARPWTFKPGQHLYLYMPRLALWTSHPFTIAWSENEQYIKFDEEKLSLHRQDIAHVGQSTLSLVIRRRTGFTDTLYKKAYGTPEGRFTTRALVEGPYGKVDTLGSYGTVVLIAGGIGITHPVPYVRELLDGYAKGTVATRRITLVWAIQSPEHLEWVRPWMTDILAMEKRREVLKVLLFVTRPKSTREIHSPSDTVQMFPGRPNIDTLLDMEIDQKMGKVAVAVCGSGSLSDDVRRAVRGKTHKANIDFIEEAFSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIA1</accession>
    <name>D5GIA1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00008362001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430324">
        <property type="protein sequence ID" value="CAZ84244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840053.1">
        <property type="nucleotide sequence ID" value="XM_002840007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIA1"/>
    <dbReference type="FunCoup" id="D5GIA1">
        <property type="interactions" value="127"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GIA1"/>
    <dbReference type="GeneID" id="9181962"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008362001"/>
    <dbReference type="eggNOG" id="ENOG502RZJ2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038840_1_1_1"/>
    <dbReference type="InParanoid" id="D5GIA1"/>
    <dbReference type="OMA" id="PENSHQD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016872">
        <property type="term" value="F:intramolecular lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016087">
        <property type="entry name" value="Chalcone_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016088">
        <property type="entry name" value="Chalcone_isomerase_3-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036298">
        <property type="entry name" value="Chalcone_isomerase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47284">
        <property type="entry name" value="FATTY-ACID-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47284:SF3">
        <property type="entry name" value="FATTY-ACID-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16035">
        <property type="entry name" value="Chalcone_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54626">
        <property type="entry name" value="Chalcone isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chalcone isomerase" evidence="2">
        <location>
            <begin position="130"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ84244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="352" mass="38553" checksum="309B36F097E58858" modified="2010-06-15" version="1">MFAARLLPRRLCRPVQATLRQTRALTLPQPTPRRPPPFHRPPSPSREELHATKHRQTILTIGAVTSMIALYVLAKQSPQQSLDSLPHPPIQPGAPVILTTDEEKRAGVELVPSGTTSVPDFPKHLEIEDKEYTLVGLGIRTVSFLGIQVYVVGLYVLTSDLPALSTYLLQYAAPSGATSTIASERDSLRALLLSPEHSERIFTTLLKNSHISTALRIVPTRNTDFSHLRDGWVRSITARAKQGGVYEDPGWAAALDEFKRLFGGRGSVPKKKTLLLVRNGEGRMTCWYDPQGRGDGKLGSGTMMLGDIRDYRVGLALWLGYLAGEKVASVPARESMVEGLVELVGRPVGSVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIB8</accession>
    <name>D5GIB8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00008416001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the D-alanine--D-alanine ligase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430325">
        <property type="protein sequence ID" value="CAZ84261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840070.1">
        <property type="nucleotide sequence ID" value="XM_002840024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIB8"/>
    <dbReference type="STRING" id="656061.D5GIB8"/>
    <dbReference type="GeneID" id="9184620"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008416001"/>
    <dbReference type="eggNOG" id="ENOG502QPTY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_784577_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIB8"/>
    <dbReference type="OMA" id="HGVKLCH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008716">
        <property type="term" value="F:D-alanine-D-alanine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011095">
        <property type="entry name" value="Dala_Dala_lig_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23132">
        <property type="entry name" value="D-ALANINE--D-ALANINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23132:SF23">
        <property type="entry name" value="D-ALANINE--D-ALANINE LIGASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07478">
        <property type="entry name" value="Dala_Dala_lig_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="5">
        <location>
            <begin position="133"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ84261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="381" mass="41926" checksum="0CB4C61CA5DF06A1" modified="2010-06-15" version="1">MAHSNHQSPGVSPPPLPHSTHPAFTIHRLTHTKSGYRDACADIAYAVHSLNDPNTNIITPQPHPNPATEIDWSFPDTRRGILAAIAAGANTIWANTCLFADHPLTTVSLPPSTKLIANPPRVVHAVDDKAFTNKLLQRHGFSIPKSWLLEGDSGRSIELLSQLIYDLPYPVIVKPVRGRGSEGVQLIDGFVSMLDTLDDLFKTYDVLLIEEFLEGEEGTVTVVPDGQGRFVALPIVQRFGQVAGVMPWNGHVPVTTNSTVLPVGEEDLWHRAAKIECQKAAELLKLTSITRIDIRRKDENGGKFFLFDVNPKPNLTGTGRPGRDSQDSLCAMAAREFGWEYPALVRKCIDAAYPLKEARAIEPPEAVTGSLDNFTVWERWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIL0</accession>
    <name>D5GIL0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00008545001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430327">
        <property type="protein sequence ID" value="CAZ84353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840162.1">
        <property type="nucleotide sequence ID" value="XM_002840116.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIL0"/>
    <dbReference type="GeneID" id="9183287"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008545001"/>
    <dbReference type="HOGENOM" id="CLU_2122864_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIL0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ84353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="114" mass="12656" checksum="5D97F33F8FFCA193" modified="2010-06-15" version="1">MCRLGRERGRVKGILTNSEGGIEAKRKKREKKRKEINFYDSLAFTCVTPTLQQFMPHHATTFYFSFFFGSGHRGSLDSPHFGTVHTSSPSSQNGESGTLWAKCLIRSLEGLGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIM9</accession>
    <name>D5GIM9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00008575001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the translin family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430327">
        <property type="protein sequence ID" value="CAZ84372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840181.1">
        <property type="nucleotide sequence ID" value="XM_002840135.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIM9"/>
    <dbReference type="STRING" id="656061.D5GIM9"/>
    <dbReference type="GeneID" id="9183306"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008575001"/>
    <dbReference type="eggNOG" id="KOG3066">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067225_2_1_1"/>
    <dbReference type="InParanoid" id="D5GIM9"/>
    <dbReference type="OMA" id="DTCMETC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14820">
        <property type="entry name" value="TRAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.190">
        <property type="entry name" value="Translin, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.200">
        <property type="entry name" value="Translin, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016069">
        <property type="entry name" value="Translin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002848">
        <property type="entry name" value="Translin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016068">
        <property type="entry name" value="Translin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036081">
        <property type="entry name" value="Translin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10741">
        <property type="entry name" value="TRANSLIN AND TRANSLIN ASSOCIATED PROTEIN X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01997">
        <property type="entry name" value="Translin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74784">
        <property type="entry name" value="Translin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ84372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="272" mass="30500" checksum="4A004FC7B0C97984" modified="2010-06-15" version="1">MPTSQRSGEPHRPKKIPTQEQQSNPYLSMFNHFRDELDEHHDCRERVIKASRDITALSKKMIFTVQRAREPFSPLPAPLSEEYETRFKQVQSIISIVSPDLQDISTYRYARQISGGIQEYIEATAFHHYLTTGKLIPLSEVQQSVKSPVEITPGDYILGIFDLIGEMMRFSITMIATRGGADKDEKVAKALRDLRELRLEFEGLDTTLGGGSGLLGKEVQKKLGVMKTCVEKVETAVCGVIVRGSERPKGWIPDLTGAVGGGGRMDRIYEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJ25</accession>
    <name>D5GJ25_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00008790001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840327.1">
        <property type="nucleotide sequence ID" value="XM_002840281.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJ25"/>
    <dbReference type="STRING" id="656061.D5GJ25"/>
    <dbReference type="GeneID" id="9181859"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008790001"/>
    <dbReference type="eggNOG" id="KOG0364">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109065_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJ25"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ84518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="221" mass="24474" checksum="66729F180FC6179B" modified="2010-06-15" version="1">MPLFFLLHYSDIVAVQVFRSSFAVTLTVAFGSLRRARKTGNNRVSRAVGVLLEIEKISDEYFSSITKCEDPEACTILLCGPSMVILYEIKRNPQDAVAVARNGFFYCWLASEGGAVEVDISVHLAEISESIEGMQQWPYKGVVEALELRTKHLAKEHARGIDGGTGKIVDMKEYGVWEPEAVMPQSVKTAIESTWLQLRVDEICSAKGDKQSGRAGRGQEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJ35</accession>
    <name>D5GJ35_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Probable endonuclease LCL3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Probable endonuclease lcl3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">GSTUM_00008821001</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the LCL3 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840337.1">
        <property type="nucleotide sequence ID" value="XM_002840291.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJ35"/>
    <dbReference type="FunCoup" id="D5GJ35">
        <property type="interactions" value="13"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJ35"/>
    <dbReference type="GeneID" id="9181869"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008821001"/>
    <dbReference type="eggNOG" id="ENOG502RZZQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046484_0_1_1"/>
    <dbReference type="InParanoid" id="D5GJ35"/>
    <dbReference type="OMA" id="IYHTPGG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.90:FF:000029">
        <property type="entry name" value="Probable endonuclease lcl3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016071">
        <property type="entry name" value="Staphylococal_nuclease_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12302">
        <property type="entry name" value="EBNA2 BINDING PROTEIN P100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12302:SF3">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00565">
        <property type="entry name" value="SNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00318">
        <property type="entry name" value="SNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50199">
        <property type="entry name" value="Staphylococcal nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50830">
        <property type="entry name" value="TNASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="TNase-like" evidence="16">
        <location>
            <begin position="77"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAZ84528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="257" mass="29028" checksum="2028B1B140B29362" modified="2010-06-15" version="1">MKWPLSSSDDNSGRIGRFTERVRSGELFTAEVFLSPDVLFPTVALTSGTLAAIYFYSHYVRRIPMTSEIYPSYFRRRSLFGTVTSVGDGDNFRLFHTPGGRLMGWGWMPGRKVPTVRKDLRDVTLHLRIAGIDAPEGAHFGKTAQPGSGEALAWLRSYILNHRVRAYIYRRDQYERVIASVKVRKGLSRRDVGLEMLKAGMATIYESRIGAEFGSLEREYRDAEAAAKKQGIGIWAPSKKQFISPGAYKKELRERGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJ68</accession>
    <name>D5GJ68_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">U6 snRNA phosphodiesterase</fullName>
            <ecNumber evidence="5">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">USB1</name>
        <name evidence="7" type="ORF">GSTUM_00008864001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Phosphodiesterase responsible for the U6 snRNA 3' end processing. Acts as an exoribonuclease (RNase) responsible for trimming the poly(U) tract of the last nucleotides in the pre-U6 snRNA molecule, leading to the formation of mature U6 snRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 2H phosphoesterase superfamily. USB1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="5"/>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840370.1">
        <property type="nucleotide sequence ID" value="XM_002840324.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJ68"/>
    <dbReference type="STRING" id="656061.D5GJ68"/>
    <dbReference type="GeneID" id="9185929"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008864001"/>
    <dbReference type="eggNOG" id="KOG3102">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050234_1_0_1"/>
    <dbReference type="InParanoid" id="D5GJ68"/>
    <dbReference type="OMA" id="FHVSIGW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990838">
        <property type="term" value="F:poly(U)-specific exoribonuclease activity, producing 3' uridine cyclic phosphate ends"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034477">
        <property type="term" value="P:U6 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1140.10">
        <property type="entry name" value="Cyclic phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03040">
        <property type="entry name" value="USB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027521">
        <property type="entry name" value="Usb1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13522">
        <property type="entry name" value="U6 SNRNA PHOSPHODIESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13522:SF3">
        <property type="entry name" value="U6 SNRNA PHOSPHODIESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09749">
        <property type="entry name" value="HVSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="1 5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="303"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="316"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="5">
        <location>
            <position position="143"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="5">
        <location>
            <position position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ84561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="326" mass="36559" checksum="AE33E0D5340E3DCD" modified="2010-06-15" version="1">MAPLTSSLVEYSDSDSDGSPPAKMAKTTYLTPANKSPTSTSKYSKASSSNSLPPLPSRFHDLYAVAPRVGQQDDPSLHGGRKRSIPHIQGNWPTHIFIEWHLSRSEFDVLNGAYHTASTITATAGVRLESHLKSDLGSEQPLHLSLSRPNILTTAQREGFLELLKDRLDKTRIKPFSVEFTGFEFVSNNDRTRWFFVLRATKGDDAQLPRLLQLANHTFEAFEQPPLYTENGDKLDGFHVSLAWSLTEPDKDVKAEVFEALKTHACWDKIFGKSEVERLKLLIDAIKVKIGNIVHIVELGKKEKQGEAGNPKKRRASYDVHEKRNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJC0</accession>
    <name>D5GJC0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="12">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00008932001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430330">
        <property type="protein sequence ID" value="CAZ84613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840422.1">
        <property type="nucleotide sequence ID" value="XM_002840376.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJC0"/>
    <dbReference type="FunCoup" id="D5GJC0">
        <property type="interactions" value="404"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJC0"/>
    <dbReference type="GeneID" id="9185981"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008932001"/>
    <dbReference type="eggNOG" id="KOG0064">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007587_1_0_1"/>
    <dbReference type="InParanoid" id="D5GJC0"/>
    <dbReference type="OMA" id="IHDMYLD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005324">
        <property type="term" value="F:long-chain fatty acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015910">
        <property type="term" value="P:long-chain fatty acid import into peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042760">
        <property type="term" value="P:very long-chain fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03223">
        <property type="entry name" value="ABCD_peroxisomal_ALDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050835">
        <property type="entry name" value="ABC_transporter_sub-D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384:SF67">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY D MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384">
        <property type="entry name" value="ATP-BINDING CASSETTE, SUB-FAMILY D MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06472">
        <property type="entry name" value="ABC_membrane_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="28"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="518"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="833"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="734"/>
            <end position="778"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ84613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="894" mass="98894" checksum="CD0E4770DC636D38" modified="2010-06-15" version="1">MANLSTLRPSLVILLDQYTKLLRSSPKSLRILAATVLCTLFGCLYERRRRRKADEGRGRVLLRRNSAIQLKDGSRLIYVPYKQTTAKVIIKPTKSTTFDAHRRLFLEPLGARKSRSFGGADVGEGPPVNTKPGLNLAFLHQFLSLLSIMIPRLTSKESLLLLLHTFFLISRTYLSLVVARLDGEIVRDLVAGNGKAFSWGIVKWLGVGGPAVYCNAMIKYLQSKISIAFRTRLTRYIHDLYLNDRQAYYKLGNLDGGLTGGGADQFITSDLPMFCDSAAALYSSLGKPFLDLVVFNYQLMRSLGPMALAGITLNYGGTAWMLKKFSPPFGRLAAAQAKREGDFRGLHTRLIGNAEEVAFYGGADMEKVFLEKGFKDLKQIMEGIYNVKIQYNMLEDFVLKYSWSAFGYLITSLPVFLPAWGGLGGVLELAGTEPAGREKDRMKGFITNKRLMLSLADAGGRMMYSFKDLSELAGYTSRVYTLVSTLHRVHSQAYSTPRPELYGMADIQGTLHKGYDGVRLEGVPIVAPGLWPRGGEELVESLDVTVREGEHLLILGPNGVGKSSIARIVAGLWPVYRGLVSRPRGGGMDGIMFLPQRVYLAQGTLRDQVIYPHTEMEMRADGRSDHELKGILDAVRLRRLPDREGGWDCRKEWKDVLSGGEKQRISLARLLYHEPKYAFIDEGTSAVSQDVEGLLYETAKEHGITLITISSRAQLKKYHTFQLQLDGGGWEFDRIGTERERSSVEHELRELKEKMKSVEGLRDRLKDIEDELARVFVQGGEELPAYEETEQGSSELGESAVVVEGEGLEPAKGVDMRGEGFAGVVGMAVGMENSGQEEEEEKGESSKDASEPAVPKVGAEPAKPVDLENGSFADTVGTDPGEDQFTPALVGLRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJE7</accession>
    <name>D5GJE7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00008964001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430330">
        <property type="protein sequence ID" value="CAZ84640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840449.1">
        <property type="nucleotide sequence ID" value="XM_002840403.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJE7"/>
    <dbReference type="GeneID" id="9182641"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008964001"/>
    <dbReference type="eggNOG" id="KOG0065">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_35_1_1"/>
    <dbReference type="InParanoid" id="D5GJE7"/>
    <dbReference type="OMA" id="HHEDAIT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03233">
        <property type="entry name" value="ABCG_PDR_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000881">
        <property type="entry name" value="ABC multidrug transporter A-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029481">
        <property type="entry name" value="ABC_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034001">
        <property type="entry name" value="ABCG_PDR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010929">
        <property type="entry name" value="PDR_CDR_ABC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19241">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14510">
        <property type="entry name" value="ABC_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06422">
        <property type="entry name" value="PDR_CDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="513"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="546"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="588"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="622"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="651"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="759"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="140"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="24"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="39"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ84640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="848" mass="94854" checksum="C70E60E5D58BE9B1" modified="2010-06-15" version="1">MSGFGEKEGVREVDLGPQNRLPPSISSSEETATYNHPNVHHEDAITPEAERDVVALARKLTKMSTSPTVIDNPFNSTDPRLQPNSDQFNVKIWVKTLIQIISRDPELYPNRTAGVSFRNLSAHGFGSQQDYQKDVSNLWMGGIDVLRNIVGGGRPGGKRGERKIQILRDFDGLVKSGEMLVVLGRPGSGCTTFLKTIAGETHGFYLDSESDIEYQGISHELMHHDFRGEAIYNAETDVHFPHLTVGETLSFAAQTRAPRNRFPGVTRKQYAEHMRDVVMTVFGISHTVNTKVGNDFIRGVSGGERKRVSIAEAALSGSPLQCWDNSTRGLDSATALEFVKTLRVQSEISGATVLVSMYQASQSAYDSFDKVTVLYEGRQIYFGSTENAKRFFTDMGFHCPERQTTADFLTSLTSPSERQAKPGFENRVPRAPDEFVEAWKKSDEYQKLLHDIAAYKEEFPLHGPQLDAFKKSRAAQQAKRMNVRSPYTISVPMQIKLCIERGFQRLKGDMSNFFSTVFGNLIMALILGSVFYNLKEDTSSFYSRGALLFFGVLMNAFSSSLEILTLYAQRPIVEKHSQYALYHPFSEAVASMICDLPLKILTCIFFNLTLYFMSNLRREVDAFFVFLLFSFACMLTMSMMFRTIAAVSRTIYQALAPASLLILMLVIYTGFAIPVRDMAGFMRWLNYIDPIGYAFESLMINEFHDRELPCVGFIPQGPGYEEVSGLQRSCTTAGAVPGRSTVNGDAYINTAFKYYYSHLWRNLGIMFAIMLFFLATYLIGTENISSARSKGEVLVFPRKQLKKRAAHGDSEAGTVSGAVAGSEASKAPAAIHRQTDIFHWKDEDFGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJM2</accession>
    <name>D5GJM2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00009073001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430332">
        <property type="protein sequence ID" value="CAZ84715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJM2"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009073001"/>
    <dbReference type="HOGENOM" id="CLU_3417327_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJM2"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="26" mass="2960" checksum="6DCD8AC95625BEA7" modified="2010-06-15" version="1">MRCSSSHAIEKKKKKNLGDCSLSPRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJT8</accession>
    <name>D5GJT8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00009188001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430333">
        <property type="protein sequence ID" value="CAZ84781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840590.1">
        <property type="nucleotide sequence ID" value="XM_002840544.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJT8"/>
    <dbReference type="GeneID" id="9186817"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009188001"/>
    <dbReference type="eggNOG" id="ENOG502RZ6G">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011910_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJT8"/>
    <dbReference type="OMA" id="WAIVHII"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053230">
        <property type="entry name" value="Trans_reg_galc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47654:SF5">
        <property type="entry name" value="TRANSCRIPTION FACTOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47654">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Xylanolytic transcriptional activator regulatory" evidence="3">
        <location>
            <begin position="326"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="85"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="103"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ84781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="650" mass="73683" checksum="587EAD312270AF15" modified="2010-06-15" version="1">MLVSHVDNGRLSQRPVCSHCADYKINCYYGEGKREMVRKRLQELEARTEAYEKLLRELLPGLDSAMQAAVQDALRVPRTSSIAAGKSPREQIIQESLPPDLQLDSESEPSDDWDSDDNPDDLAEDINTLSINKPIGYMGRSSEVLWINLLKEEIKKCPDSVQADEPNQLEGGVWEQGEEVGWTYHLDDIDISFSGKQIDAYALPPKDLANSLVNVYFETVHPLFPIIYKGLFKNQFELLYQTRDPGKVARKWLAIMNLVFAIGARHLSMIGENKADIWHHVTFFLRARLLGALDGGLVFEIATLQNVQAMGLTGMYLLASKQTNRSWNVAGLAIRLSYGMGLHLRNVASTLNAGQKEMRVRVWYSVCYLERTLGLITGRPSALQAHQSSAPLPRSKDLDLESDPAQDAYFAAHIKLSSIASEVLSQLYSTRSRFVMQKSWGHVQETMESKLIPSLSFNGTRDSNDDKTFMNQRLDLALRYHNIRILIFRPCICQLELGVAKEVGFSEELNRSGAQTCVNSARNIISLVADTSEDKREQLMKIPWWCVLHYLVAAEAILLLETIRQRASNVDIGIINDMRQVVKWLHGISGNDMANAGDIRVQGDEREGSRQEGERRWLRDDFALHMAEQGHMRAEDDVWDFERGFDMQYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5GK74</accession>
    <name>D5GK74_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00009411001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430337">
        <property type="protein sequence ID" value="CAZ84917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840726.1">
        <property type="nucleotide sequence ID" value="XM_002840680.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GK74"/>
    <dbReference type="GeneID" id="9186946"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009411001"/>
    <dbReference type="eggNOG" id="ENOG502SN91">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121045_0_0_1"/>
    <dbReference type="InParanoid" id="D5GK74"/>
    <dbReference type="OMA" id="MEIPWRA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF51">
        <property type="entry name" value="MYB-LIKE DNA-BINDING PROTEIN BAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH myb-type" evidence="3">
        <location>
            <begin position="29"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="29"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ84917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="193" mass="21466" checksum="DAC9B7B87C80021E" modified="2010-06-15" version="1">MPPKQKDTLPPPPPSPSTKRPLSDVAQTSPAKKQSKWSADEDAKIIDLRGRGHKWEDISRELPGRSAISCRLHYQNYLERRPEWDEERKDRLARLYERFKADMWALVAREMEIPWRAAEAMHWQLGEREMARRAGVVPFSLSNIALDAQPKRTGNVGTTGRKGQNSGSTNNSATGPSAPPTEAGSAPESQGAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>D5GK83</accession>
    <name>D5GK83_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peroxisomal membrane protein PEX16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00009421001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Peroxisome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the peroxin-16 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430337">
        <property type="protein sequence ID" value="CAZ84926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840735.1">
        <property type="nucleotide sequence ID" value="XM_002840689.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GK83"/>
    <dbReference type="STRING" id="656061.D5GK83"/>
    <dbReference type="GeneID" id="9186955"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009421001"/>
    <dbReference type="eggNOG" id="KOG4546">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036533_2_0_1"/>
    <dbReference type="InParanoid" id="D5GK83"/>
    <dbReference type="OMA" id="PTWQSTY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013919">
        <property type="entry name" value="Pex16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13299">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13299:SF0">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08610">
        <property type="entry name" value="Pex16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="2" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="146"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU365003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ84926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="349" mass="39907" checksum="710A451837576829" modified="2010-06-15" version="1">MYGDFITKNAGAVSQIESALRSLTYIVPARFKDVELASESLHSGIQILSLYHDSLLARAVTKIPPGVQKPSPHNRYTKFWTQKSPGYKKLALIVTMIQYTELLWEMSAKRRGQKMRWRAVIVLEAIKAICRLLLLRLTNSRPLLTPPLPEREIDPQTLEETLGPESSPPEETWKMPRTGLCLPSLPATNQISQYLLTKVLTAEDIKPPSQLLHRIRGTGYYAEVLYIIRPVIYALAMKKWAQDKKSWRPWLIGFSLEYAARQLSKRDFEMNVPGGMKGLTALEREEMTRRAWGMGWWGVRGAFYENVTGPWLKAISTKLADKPALNLIAGLLEDYQYLWDNYYFSTATL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKA4</accession>
    <name>D5GKA4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cytoplasmic tRNA 2-thiolation protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">NCS2</name>
        <name evidence="3" type="synonym">CTU2</name>
        <name evidence="4" type="ORF">GSTUM_00009444001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by forming a heterodimer with NCS6 that ligates sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position. Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation. May also be involved in protein urmylation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CTU2/NCS2 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430337">
        <property type="protein sequence ID" value="CAZ84947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840756.1">
        <property type="nucleotide sequence ID" value="XM_002840710.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKA4"/>
    <dbReference type="FunCoup" id="D5GKA4">
        <property type="interactions" value="169"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GKA4"/>
    <dbReference type="GeneID" id="9182542"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009444001"/>
    <dbReference type="eggNOG" id="KOG2594">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024534_3_0_1"/>
    <dbReference type="InParanoid" id="D5GKA4"/>
    <dbReference type="OMA" id="KQRKQMM"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016783">
        <property type="term" value="F:sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032447">
        <property type="term" value="P:protein urmylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002143">
        <property type="term" value="P:tRNA wobble position uridine thiolation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03054">
        <property type="entry name" value="CTU2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019407">
        <property type="entry name" value="CTU2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20882">
        <property type="entry name" value="CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20882:SF14">
        <property type="entry name" value="CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10288">
        <property type="entry name" value="CTU2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ84947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="406" mass="44720" checksum="E7068CD15E1F7D55" modified="2010-06-15" version="1">MTEQNITPNGATVPTFCKRCSTNSSVINLRTEHLCSDCFDKYVRSKCIKRMEAYRVRYNPPDTDTSPRRLLLPLSFGISSTTLLHLLNSQLQNQLARTTRTGFVLIVVHIDESAINPTVPLAEEGMRAVQEWFPDVGEYFIVRLEGIYGFSNAVKAFEMGVEEEERRGGGDWREKLKGLLGALPSPTSRMDLLQILRTRLITELARKYACEAVLWGDTTTRLAEKMLAETAKGRGFSVPWQTSDGDSPYGVKFLYPLRDLLKKELVVYVSLEVPTPLRPLCFGAAEEGEGKKKHAAVGKGITIDELMTQYFEGIEGQYPSIVANVVRTAGKLVTEGGVVRIGRCNICGLPRDGGGGELVLDAKTKLGESSSGEVNGNRSGGKKEGGDMCYGCARSVHGARPYEWPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKA8</accession>
    <name>D5GKA8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00009452001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the Tom5 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430337">
        <property type="protein sequence ID" value="CAZ84951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840760.1">
        <property type="nucleotide sequence ID" value="XM_002840714.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKA8"/>
    <dbReference type="GeneID" id="9181971"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009452001"/>
    <dbReference type="eggNOG" id="ENOG502T2K6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_209440_1_1_1"/>
    <dbReference type="InParanoid" id="D5GKA8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006626">
        <property type="term" value="P:protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019603">
        <property type="entry name" value="Tom5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10642">
        <property type="entry name" value="Tom5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ84951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="49" mass="5381" checksum="F76A975A009CEBEB" modified="2010-06-15" version="1">MFGVGPPPPSAAEIREQEREAKNAIQGAVKTCILLYLSPFVVDYVKSFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKH3</accession>
    <name>D5GKH3_TUBMM</name>
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        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
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        <name evidence="2" type="ORF">GSTUM_00009559001</name>
    </gene>
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        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430339">
        <property type="protein sequence ID" value="CAZ85016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002840779.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKH3"/>
    <dbReference type="GeneID" id="9182035"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009559001"/>
    <dbReference type="HOGENOM" id="CLU_2759653_0_0_1"/>
    <dbReference type="InParanoid" id="D5GKH3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="70" mass="8042" checksum="7E31531784D35842" modified="2010-06-15" version="1">MTYVHPRKAALGDSRLDRPSVPTENPGTDFTPPPRPTECHRGCRTRVNPALFFRWCCFRTPHQNSSCENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>D5GL98</accession>
    <name>D5GL98_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00010083001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430347">
        <property type="protein sequence ID" value="CAZ85291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841100.1">
        <property type="nucleotide sequence ID" value="XM_002841054.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GL98"/>
    <dbReference type="STRING" id="656061.D5GL98"/>
    <dbReference type="GeneID" id="9182085"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010083001"/>
    <dbReference type="eggNOG" id="ENOG502SQUP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_861049_0_0_1"/>
    <dbReference type="InParanoid" id="D5GL98"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003807">
        <property type="entry name" value="DUF202"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052053">
        <property type="entry name" value="IM_YidH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34187:SF1">
        <property type="entry name" value="DUF202 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34187">
        <property type="entry name" value="FGR18P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02656">
        <property type="entry name" value="DUF202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="186"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="216"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="258"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="287"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="DUF202" evidence="7">
        <location>
            <begin position="169"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="77"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="39"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="89"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ85291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="323" mass="35068" checksum="9A2A008C1CB67D1F" modified="2010-06-15" version="1">MVSTSSAESSLQSQSQQQQKHHNKLQEPQPRDAFRGTHPSPNSSVFQTSTIASAAPPAIPILPMPPAGYEDAIELQQWDYGGPPHNPSDDSNCCETPRSEQTSGSTKYGYSGPATTPDDSGSSNCSTVGSPPTPDGPDASVLKTLKLLSRFAVFWDDHMSVVVPSNAARDHLALERTYLAYHRTSLVFAISAAITAQLAVIQQTPTPSTTFGFHRIGKPISILLVCFSLAISILGVLRWWKLQSGLLRGVAVTGGIEVWILASGILLLMIVLSGAALWRGYLVSRRLLPAFFLSIFFPYVTPMPYITHHLEGAQQYIRMGLHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>D5GM05</accession>
    <name>D5GM05_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">DNA replication regulator SLD2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00010344001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 8">Has a role in the initiation of DNA replication. Required at S-phase checkpoint.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the SLD2 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430351">
        <property type="protein sequence ID" value="CAZ85467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841276.1">
        <property type="nucleotide sequence ID" value="XM_002841230.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GM05"/>
    <dbReference type="STRING" id="656061.D5GM05"/>
    <dbReference type="GeneID" id="9182770"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010344001"/>
    <dbReference type="eggNOG" id="ENOG502SCF7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033089_0_0_1"/>
    <dbReference type="InParanoid" id="D5GM05"/>
    <dbReference type="OMA" id="AVRMPQK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031261">
        <property type="term" value="C:DNA replication preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902977">
        <property type="term" value="P:mitotic DNA replication preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22289">
        <property type="entry name" value="RecQL4_SLD2_NTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1460:FF:000001">
        <property type="entry name" value="DNA replication regulator Sld2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021110">
        <property type="entry name" value="DNA_rep_checkpnt_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040203">
        <property type="entry name" value="Sld2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28124">
        <property type="entry name" value="DNA REPLICATION REGULATOR SLD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28124:SF1">
        <property type="entry name" value="DNA REPLICATION REGULATOR SLD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11719">
        <property type="entry name" value="Drc1-Sld2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 8" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="168"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="283"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="364"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="53"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="110"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="239"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="331"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="396"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="409"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ85467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="461" mass="51483" checksum="4BFC83F933E1F2F3" modified="2010-06-15" version="1">MPTVDDNRAKQLRAELKEWEHSFAAEHRRKPSREEVKRDPRISAKYKEYSKLRTSTSTAASRPTTSNPKPKPERTPSTTKGIRDPFASARSDKSTASTRKKAADIFKTPTKPQLTSADQQEIYDSPLSLRRTRLFGNRPRDSVGPTPQKTGNVLGLFESFVDIKVTPKKARDRVSASPVSTRKTGDTNEGGANGRGEFATPRKRKAEEISVGSAKKRGEEEFTTPTFLRRDSSRFQPPVESPPVPLPMRPVRGLGSMVAELRKMEDEAIEEEFADDEEAMKEMEALDAEPSLPPPEQRGEGLEVGIGDLPPGAFAEEAIVDENEGEDKDKPKWKKKGLKRQTRRTISNYTSPNTNILVRKLTNIVRPTTTKPQEMPQEEDLRPDPANAELPPAGYDDGEDLSNIESEDERTSKKAMVDKGKAVVKKVVRKVKATANANFRRLNIRGQGTKARGGGKFRRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GM32</accession>
    <name>D5GM32_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protection of telomeres protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00010501001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the telombin family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430351">
        <property type="protein sequence ID" value="CAZ85575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841384.1">
        <property type="nucleotide sequence ID" value="XM_002841338.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GM32"/>
    <dbReference type="STRING" id="656061.D5GM32"/>
    <dbReference type="GeneID" id="9187359"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010501001"/>
    <dbReference type="eggNOG" id="KOG4757">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016663_1_0_1"/>
    <dbReference type="InParanoid" id="D5GM32"/>
    <dbReference type="OMA" id="WEPHASF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000783">
        <property type="term" value="C:nuclear telomere cap complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098505">
        <property type="term" value="F:G-rich strand telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010521">
        <property type="term" value="F:telomerase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032210">
        <property type="term" value="P:regulation of telomere maintenance via telomerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016233">
        <property type="term" value="P:telomere capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04497">
        <property type="entry name" value="hPOT1_OB1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000303">
        <property type="entry name" value="Protection of telomeres protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028389">
        <property type="entry name" value="POT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032042">
        <property type="entry name" value="POT1PC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011564">
        <property type="entry name" value="Telomer_end-bd_POT1/Cdc13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14513">
        <property type="entry name" value="PROTECTION OF TELOMERES 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14513:SF0">
        <property type="entry name" value="PROTECTION OF TELOMERES PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02765">
        <property type="entry name" value="POT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16686">
        <property type="entry name" value="POT1PC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00976">
        <property type="entry name" value="Telo_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0779">Telomere</keyword>
    <feature type="domain" description="Telomeric single stranded DNA binding POT1/Cdc13" evidence="10">
        <location>
            <begin position="10"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="480"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="502"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ85575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="586" mass="66449" checksum="A9CB36285E6BFF8C" modified="2010-06-15" version="1">MAGAPLPKGCVSIATAVDTKIGECCDLAGVVVDFRPPVATRGTDWMATITLCDRSRALPETSLTIRIFRPNMELLPEIKSDSDLILIRRVKVIKHDGRLLALSTFDTSWIISYLPTQKGIPSIASYPSSLKIKADELAYFQGLRVWWKSRRESFVSKGAAPGTCPSYQLGSSSQRRRSGLIRDMRIGSFYDLAGVVVKTFPGNGNYTVYLTDYTKNSMLHSYEWGGSRRIGAGGDDDDFDYTGRGRGLGNQDWPGPWGQYTLQVTLWDNHAVAANRLFYVGAHVSLQNVRAKRNGDGKLEGTLNGDRQFPDKVLVSLIKDMNDPRVKQIAIRCKEYTRRFEDDRLRYEKEMMEVNAHIRALKASQPITPISEIAEPQDLDGPFANRIYKIRCRVIDYLPQQLEDFAVLQTKHSGWTWRFALLVEGEDGATLRVIVEGPDAEYLLGRPAANLRTDRQELSALREKLFILWGNLREVKGRQLESRTPRKNAAFQSDTPPEAPCGRKRRRRRNTKQNQVAHKHLKYDELGRRLNADRETDDDECQDENEEHPILQLSGSIFDACLKEYGVLSVDGQWKRVHRLFGTRIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>D5GM58</accession>
    <name>D5GM58_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00010537001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430352">
        <property type="protein sequence ID" value="CAZ85601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841410.1">
        <property type="nucleotide sequence ID" value="XM_002841364.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GM58"/>
    <dbReference type="STRING" id="656061.D5GM58"/>
    <dbReference type="GeneID" id="9187385"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010537001"/>
    <dbReference type="eggNOG" id="KOG0955">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1195624_0_0_1"/>
    <dbReference type="InParanoid" id="D5GM58"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048189">
        <property type="term" value="C:Lid2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036205">
        <property type="term" value="P:histone catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029617">
        <property type="entry name" value="Snt2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47672">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SNT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47672:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SNT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13832">
        <property type="entry name" value="zf-HC5HC2H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ85601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="232" mass="26087" checksum="4822B84226A0E71D" modified="2010-06-15" version="1">MCANDKYPTVSTVGLPNLCLRRTVVLVLAIRILRQNVRPRKRFKWKRTSQSSIITAPMGMAVRLRKSIGVGQKPSRITEKTSGNNWTHGVCAVWTPKTKFAEATTMKIVEGIGTIPVARWLQICKICEGNGGAYVECYVSHESGKYPQSSYTSGKCRSCNTFIVHVGRAHKAGWRMGLDIQPMKGSKRGQMNMFQLGTETGSVTAAIWCPEHELKRRLLQMCLARYLRRPDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5GM81</accession>
    <name>D5GM81_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00010607001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldo/keto reductase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430353">
        <property type="protein sequence ID" value="CAZ85635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841444.1">
        <property type="nucleotide sequence ID" value="XM_002841398.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GM81"/>
    <dbReference type="FunCoup" id="D5GM81">
        <property type="interactions" value="119"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GM81"/>
    <dbReference type="GeneID" id="9187419"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010607001"/>
    <dbReference type="eggNOG" id="KOG1575">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_2_0_1"/>
    <dbReference type="InParanoid" id="D5GM81"/>
    <dbReference type="OMA" id="EREMIKY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19079">
        <property type="entry name" value="AKR_EcYajO-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000004">
        <property type="entry name" value="Oxidoreductase, aldo/keto reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050523">
        <property type="entry name" value="AKR_Detox_Biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43364">
        <property type="entry name" value="NADH-SPECIFIC METHYLGLYOXAL REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43364:SF9">
        <property type="entry name" value="OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="4">
        <location>
            <begin position="25"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ85635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="353" mass="39938" checksum="0B3630C61135F1EC" modified="2010-06-15" version="1">MTELSSQKIEYRRVGNSGLKVSVPILGAMGFGNKLWQPWCVDEGASLQVLKAAYDLGINTWDTANTYSNGESERIIGKAIRKFKIPRDKLVIMTKCYCAAGDEPGMQSYMMPELKFRKDYVNRLGLSRKAIFSEVNSSLERLGLEYIDLLQIHRCDPETPPEETMEALHDLVKSGKVRYIGASSMWTWQFCLYQQTAERNNWTKFISMQNQYSLVYREEERDMIPYCRATGVGIIPWAPLARGHLTRLPAAETDSERNHMEKECEGAAFFAVGSSEADKKTISRIKEVSDNRGWTMAEVALAWILGKDTTPIVGMAKVERVADMGGLKGKSLDESEMEYLEELYVPRKVSGHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>D5GME9</accession>
    <name>D5GME9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pre-mRNA-splicing factor CWC2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Pre-mRNA-splicing factor cwc2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">GSTUM_00010676001</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Involved in the first step of pre-mRNA splicing. Required for cell growth and cell cycle control. Plays a role in the levels of the U1, U4, U5 and U6 snRNAs and the maintenance of the U4/U6 snRNA complex. May provide the link between the 'nineteen complex' NTC spliceosome protein complex and the spliceosome through the U6 snRNA. Associates predominantly with U6 snRNAs in assembled active spliceosomes. Binds directly to the internal stem-loop (ISL) domain of the U6 snRNA and to the pre-mRNA intron near the 5' splice site during the activation and catalytic phases of the spliceosome cycle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRM CWC2 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430354">
        <property type="protein sequence ID" value="CAZ85692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841501.1">
        <property type="nucleotide sequence ID" value="XM_002841455.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GME9"/>
    <dbReference type="FunCoup" id="D5GME9">
        <property type="interactions" value="169"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GME9"/>
    <dbReference type="GeneID" id="9182118"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010676001"/>
    <dbReference type="eggNOG" id="KOG0118">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043308_1_0_1"/>
    <dbReference type="InParanoid" id="D5GME9"/>
    <dbReference type="OMA" id="CNIAKDS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071006">
        <property type="term" value="C:U2-type catalytic step 1 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036002">
        <property type="term" value="F:pre-mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017070">
        <property type="term" value="F:U6 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12360">
        <property type="entry name" value="RRM_cwf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000249">
        <property type="entry name" value="Pre-mRNA-splicing factor CWC2, variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039171">
        <property type="entry name" value="Cwc2/Slt11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034181">
        <property type="entry name" value="Cwc2_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032297">
        <property type="entry name" value="Torus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089:SF2">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR CWC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR RBM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16131">
        <property type="entry name" value="Torus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="10" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 15" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="8 16" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="19">
        <location>
            <begin position="127"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="18">
        <location>
            <begin position="189"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="16">
        <location>
            <begin position="127"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="29"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="52"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00072313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CAZ85692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="418" mass="45521" checksum="676B001CD2040050" modified="2010-06-15" version="1">MAESPPQSPGSTAEEMREPTPDAPAAGTNPDSAVANTTAGGDSAAAPTALTKPKKSTKKKRRPARPQVDPSTFKGGEIPAQTGTVFNIWYNKWSGGDREDKYISKSHAEGRCNIARDSGYTKADKVPGSYFCLFFARGLCPRGADCEYLHRLPTVTDLFNPNVDCFGRDKHSDYRDDMGGVGSFMRQNHTLYVGRIHVSDEIEEIVARHFAEWGQIERIRVLNSRGVGFVTYSNEANAQFAKEAMAHQSLDHNEILNVRWATTDPNPMAQAREKRRIEEQAAAAIRQALPEEFVAELEGKNKDASKKRRIEGSFGLKGYEVPEEIWYARGPNAVNPAGRGVEGQVDGLMIERAVGSNGVGDEVADRQTKEQVGIFSSQTLEALRGLNRGSNGPPRVTMKGSNVSAGLALAADYGSDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMQ5</accession>
    <name>D5GMQ5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00010886001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430359">
        <property type="protein sequence ID" value="CAZ85798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841607.1">
        <property type="nucleotide sequence ID" value="XM_002841561.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMQ5"/>
    <dbReference type="STRING" id="656061.D5GMQ5"/>
    <dbReference type="GeneID" id="9185134"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010886001"/>
    <dbReference type="eggNOG" id="ENOG502RX7P">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057565_0_0_1"/>
    <dbReference type="InParanoid" id="D5GMQ5"/>
    <dbReference type="OMA" id="PRIECQL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000935">
        <property type="term" value="C:division septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000917">
        <property type="term" value="P:division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053060">
        <property type="entry name" value="Cytokinesis_Signaling_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36419">
        <property type="entry name" value="ARRESTIN FAMILY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36419:SF1">
        <property type="entry name" value="RHO1 GEF LOCALIZING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01017">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="1">
        <location>
            <begin position="190"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="406" mass="45012" checksum="A5BAA0D795A27694" modified="2010-06-15" version="1">MEGSKVNAFVRVSGPPNRNYLVGYPGISATLPRIEGKVEIRPGAGYSAPVGISYVAIGLYRKETIHPHADSIISNHLAAPRKEIKDLVGKEIRLFQCSAGKAYDSVLAMDLPFVLFIPFSKAGNEDIIKVPPASLQLPSRVAETYYELVVSVQQGSADLKKHSFPVPLSRYDTLSSFGMYNRPETQERVSDHLVTLSMCLQRWSFGPNDPVEVRVRLTPNPDWMSKAKKVSIQRLVVVIEEQITYNPEGDEPTTKVNKLITKKELVGQKLPPDGYQSDITIRFPAKELRDSDGFLPPPKPAFPMHPIVGFTTTATLYKIDYFLSVKAHMSSCRDITLRQRIIVSPFDHQTCLDELPSIMQAAQDATGINGAGPMLPAPTIVRPGDLNALHWLGMTMVGNERKLLIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5GN06</accession>
    <name>D5GN06_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sulfhydryl oxidase</fullName>
            <ecNumber evidence="6">1.8.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00011001001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2</text>
            <dbReference type="Rhea" id="RHEA:17357"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:16520"/>
            <dbReference type="ChEBI" id="CHEBI:17412"/>
            <dbReference type="EC" id="1.8.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="1.8.3.2" evidence="6"/>
    <dbReference type="EMBL" id="FN430361">
        <property type="protein sequence ID" value="CAZ85881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841690.1">
        <property type="nucleotide sequence ID" value="XM_002841644.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GN06"/>
    <dbReference type="FunCoup" id="D5GN06">
        <property type="interactions" value="13"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GN06"/>
    <dbReference type="GeneID" id="9183449"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011001001"/>
    <dbReference type="eggNOG" id="KOG3355">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070631_2_1_1"/>
    <dbReference type="InParanoid" id="D5GN06"/>
    <dbReference type="OMA" id="MARKPHF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016971">
        <property type="term" value="F:flavin-dependent sulfhydryl oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060904">
        <property type="term" value="P:regulation of protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.310:FF:000002">
        <property type="entry name" value="Sulfhydryl oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.310">
        <property type="entry name" value="ERV/ALR sulfhydryl oxidase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039799">
        <property type="entry name" value="ALR/ERV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036774">
        <property type="entry name" value="ERV/ALR_sulphydryl_oxid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017905">
        <property type="entry name" value="ERV/ALR_sulphydryl_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12645">
        <property type="entry name" value="ALR/ERV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12645:SF1">
        <property type="entry name" value="FAD-LINKED SULFHYDRYL OXIDASE ERV2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04777">
        <property type="entry name" value="Evr1_Alr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69000">
        <property type="entry name" value="FAD-dependent thiol oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51324">
        <property type="entry name" value="ERV_ALR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 6" id="KW-0274">FAD</keyword>
    <keyword evidence="2 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072465" description="Sulfhydryl oxidase" evidence="8">
        <location>
            <begin position="30"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="ERV/ALR sulfhydryl oxidase" evidence="9">
        <location>
            <begin position="65"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="175"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="175"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU371123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ85881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="216" mass="23661" checksum="9FE2FD4111576462" modified="2010-06-15" version="1">MTRNKPFLLATFFTLFLFFSYILLPSTKPTPPPPPPHLPTTALAQPPPIPSSILAGSAIMPHLGNETLKAELGRAGWKLFHMTLARFPESPSLDERTALAQYLALFARLYPCGECAEHFQKLLAQYPPQTSGRVAASQWGCFVHNLVNERLGKEVFDCMTVGEAYKCGCAEEDEKKGKGEEVVKGRKGKGVVKTTGSSLEENLEGSGRKIEVIKEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNA1</accession>
    <name>D5GNA1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00011190001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430362">
        <property type="protein sequence ID" value="CAZ85994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841803.1">
        <property type="nucleotide sequence ID" value="XM_002841757.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNA1"/>
    <dbReference type="STRING" id="656061.D5GNA1"/>
    <dbReference type="GeneID" id="9182193"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011190001"/>
    <dbReference type="eggNOG" id="KOG0815">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111339_1_0_1"/>
    <dbReference type="InParanoid" id="D5GNA1"/>
    <dbReference type="OMA" id="NIASACL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050323">
        <property type="entry name" value="Ribosomal_protein_uL10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45699">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN P0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45699:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00428">
        <property type="entry name" value="Ribosomal_60s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="87"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="88"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="102"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="112"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ85994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="129" mass="13825" checksum="CD76EC9D5A4D6E9A" modified="2010-06-15" version="1">MGISQVYDKGQVFAPSVLDIDDEQLLASFLNAVKTLATISLAVNYPTLPSVIHSLVNSYKKAIAVALETEYSWEAIDELKDRIANPEAYASAGPAPGATAAEDSKPEAKEEEKEDEESEDEGGFGDLFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNN7</accession>
    <name>D5GNN7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00011390001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the REXO1/REXO3 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430366">
        <property type="protein sequence ID" value="CAZ86130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841939.1">
        <property type="nucleotide sequence ID" value="XM_002841893.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNN7"/>
    <dbReference type="FunCoup" id="D5GNN7">
        <property type="interactions" value="205"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GNN7"/>
    <dbReference type="GeneID" id="9186206"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011390001"/>
    <dbReference type="eggNOG" id="KOG2248">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008679_1_0_1"/>
    <dbReference type="InParanoid" id="D5GNN7"/>
    <dbReference type="OMA" id="HKAGPPF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06145">
        <property type="entry name" value="REX1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000019">
        <property type="entry name" value="RNA exonuclease NEF-sp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034922">
        <property type="entry name" value="REX1-like_exo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047021">
        <property type="entry name" value="REXO1/3/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801:SF115">
        <property type="entry name" value="FI18136P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801">
        <property type="entry name" value="RNA EXONUCLEASE REXO1 / RECO3 FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exonuclease" evidence="8">
        <location>
            <begin position="299"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ86130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="662" mass="72691" checksum="3AEFB820FD589851" modified="2010-06-15" version="1">MGKRKHRNAFPPPKASSSSSNARDAPSPPPLPAIKFGEFKLTSTSGPFTPNPVTTTTITNTATTTATTVNSMGREVGEDDGGGEWQIAKRHKKSKHKYPSFEVSPARLKNAVKLSDIQSLVLWLLADGTAPQWLLVKNKNEIRGVVVVMVPGLEMAIPGSKPGLGWGKRAFFPQELKKAQLQVCVRGFADMFSHVWPVVAPGDERGRVYSAIAGFLNTPIPKGKAPQDPDKYTTSQRIGITRLVMPFGELVENEYPVHSVHATTSSVAETTKVGWVETDLSRGNGRENNEGGSVTEAKTIYAMDCEMVQTKQGLELVRISLVSWDGETIYDTLVKPDSPITDYLTPRYSGVTKAMLDPVTTSLKDVQNHLLRLLNNDTILVGQSLNADLSAIKIAHPHIVDTSVIYNHPRGPPYRASLKWLSTKHLKREIQKDGSNGHDSIEDAKACLDLLKLKLERGLEYGTSSAAAESIFKRLSRVRPVPRIGGVVDYGTPDKWWSPSAARVIQAKDDAEVATGIVKSICGDPPGTRDGHPKLDLVWGRMHALEALRGWNKAAAPEEGPPIPIENTESNHAVIAQGVRDVATQLKQIYDSLPPCTAFIVYSGTGDPRELRRLQDMQREHKRQYMTMKWDELTVKWTDTENRALAAAANTTREGIAFLTVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5GP08</accession>
    <name>D5GP08_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00011611001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the Mrp/NBP35 ATP-binding proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430371">
        <property type="protein sequence ID" value="CAZ86251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842060.1">
        <property type="nucleotide sequence ID" value="XM_002842014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GP08"/>
    <dbReference type="STRING" id="656061.D5GP08"/>
    <dbReference type="GeneID" id="9183544"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011611001"/>
    <dbReference type="eggNOG" id="KOG3022">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024839_0_2_1"/>
    <dbReference type="InParanoid" id="D5GP08"/>
    <dbReference type="OMA" id="CNHESHI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140663">
        <property type="term" value="F:ATP-dependent FeS chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02037">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001278">
        <property type="entry name" value="Iron-sulfur cluster carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02040">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000808">
        <property type="entry name" value="Mrp-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019591">
        <property type="entry name" value="Mrp/NBP35_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044304">
        <property type="entry name" value="NUBPL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033756">
        <property type="entry name" value="YlxH/NBP35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42961">
        <property type="entry name" value="IRON-SULFUR PROTEIN NUBPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42961:SF2">
        <property type="entry name" value="IRON-SULFUR PROTEIN NUBPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10609">
        <property type="entry name" value="ParA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01215">
        <property type="entry name" value="MRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ86251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="294" mass="31341" checksum="BC76A8799F1FEE81" modified="2010-06-15" version="1">MSLKRLFTTSRSLTHENPLGLPRSGSPPQIPRILRGLPTKRPIKDVSKVVAVSSAKGGVGKSTIAVNLALALARQGKRTGILDADIFGPSIPKLLNLSGEPRLSSHNQLIPLSNYGVKSMSMGYLAGEEAPVIWRGLMVMKAMNQMLNEVEWGGLDVLVLDLPPGTGDVQLSIAQQVVVDGALIVSTPQDIALVDAVKGINMLKKVDVPLLGMVQNMSVFTCPNCQHVTHVFGADGVERECKKHGIELLGDIPLHASICQDADRGKPTVVSDPTGAHALAFEGISERVSRKLFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5GP11</accession>
    <name>D5GP11_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00011617001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the KRI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430371">
        <property type="protein sequence ID" value="CAZ86254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842063.1">
        <property type="nucleotide sequence ID" value="XM_002842017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GP11"/>
    <dbReference type="FunCoup" id="D5GP11">
        <property type="interactions" value="573"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GP11"/>
    <dbReference type="GeneID" id="9183547"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011617001"/>
    <dbReference type="eggNOG" id="KOG2409">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009647_3_0_1"/>
    <dbReference type="InParanoid" id="D5GP11"/>
    <dbReference type="OMA" id="YAMEDRE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018034">
        <property type="entry name" value="Kri1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024626">
        <property type="entry name" value="Kri1-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14490:SF5">
        <property type="entry name" value="PROTEIN KRI1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14490">
        <property type="entry name" value="ZINC FINGER, ZZ TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05178">
        <property type="entry name" value="Kri1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12936">
        <property type="entry name" value="Kri1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kri1-like C-terminal" evidence="3">
        <location>
            <begin position="331"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="63"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="155"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="275"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="425"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="168"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="280"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="294"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="455"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ86254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="466" mass="54019" checksum="DA754D4274DB549D" modified="2010-06-15" version="1">MFLKDYHRKNLLSGTVGDEETPMTYLQEQEDFKRTVVLEMHQATANEPDSDPDDADAGFLVRKRQPEKPEPVVDIPDPATADPDNPEEYLTRFFQSRAWTKPTGFIPLEDDDSGEEDCIEEFEKAYNFRFEDPDANVRGKLMTYGRDAISANTVRRGEISGRKKTREKKREKQQAEKELREKEKGRLRKLKTEELMEKFRMIREAAGLDDDGEDKEVEAEVLNNLLEGDWSDEQWEEWMAKKFGDSYYEANVGKIKKPKFDGEIDIGDIVSDLEGGVEFGLEDGGGEADSETSEGDKEVGKDSQTDRESRDVGPFQKEERKKRQKERDLKRKLEQYIEENYDFEDQIPSSNLKTSFRYRETTPESYGLTSLDILAAEDADLNSFVGLRKLATFREPEKKKRDKKRYGKKKRLREWRKETFGDEEGVKIPADQEPTGVTEEREYYVDIRGVGGGERKKKRRRKGNKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPH1</accession>
    <name>D5GPH1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">U4/U6 snRNA-associated-splicing factor PRP24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00011843001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the cleavage factor IA (CFIA) complex, which is involved in the endonucleolytic cleavage during polyadenylation-dependent pre-mRNA 3'-end formation.</text>
    </comment>
    <comment type="function">
        <text evidence="8">Functions as a recycling factor of the spliceosome, a machinery that forms on each precursor-messenger RNA (pre-mRNA) and catalyzes the removal of introns. Chaperones the re-annealing of U4 and U6 snRNAs (small nuclear RNAs) released from previous rounds of splicing, an initial step in reforming the U4/U6-U5 tri-snRNP (small nuclear ribonucleoprotein) that can reassemble into another spliceosome complex; this step involves binding U6 and facilitating the unwinding of the U6 internal stem loop, followed by base-pairing of U6 to U4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430375">
        <property type="protein sequence ID" value="CAZ86414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842223.1">
        <property type="nucleotide sequence ID" value="XM_002842177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPH1"/>
    <dbReference type="STRING" id="656061.D5GPH1"/>
    <dbReference type="GeneID" id="9186654"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011843001"/>
    <dbReference type="eggNOG" id="KOG0128">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003925_0_0_1"/>
    <dbReference type="InParanoid" id="D5GPH1"/>
    <dbReference type="OMA" id="LWARYIL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005688">
        <property type="term" value="C:U6 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12296">
        <property type="entry name" value="RRM1_Prp24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12297">
        <property type="entry name" value="RRM2_Prp24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12299">
        <property type="entry name" value="RRM4_Prp24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000588">
        <property type="entry name" value="Pre-mRNA splicing factor (Prp24), putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000365">
        <property type="entry name" value="U4/U6 snRNA-associated-splicing factor PRP24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050502">
        <property type="entry name" value="Euk_RNA-bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034397">
        <property type="entry name" value="Prp24_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034398">
        <property type="entry name" value="Prp24_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031766">
        <property type="entry name" value="RRM_occluded"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008847">
        <property type="entry name" value="Suf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025">
        <property type="entry name" value="OS02G0815200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025:SF1">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16842">
        <property type="entry name" value="RRM_occluded"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05843">
        <property type="entry name" value="Suf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 10" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="670"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="746"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="837"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="968"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="596"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="915"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1096"/>
            <end position="1125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="917"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="930"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1096"/>
            <end position="1105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00093374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00093627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ86414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1133" mass="127192" checksum="1C3FC1DFEABD33E2" modified="2010-06-15" version="1">MTGGDDDDAAAAVAVGDGIGVVYEAVMEDASDGDENEVVVSVPLSEEDYTRIADLLVSITNRPYQYESHVEYISLLRRGFLAHRADPRELGPYPLIKDLRKAREDMIERFPLNETMWAEWIADEISICGGLEERLGVMELCAKAVEEESASVKLWKTYAEYIESQYSLGRGGDAAGKGKAKKLLSEEEEVTLKGCFTLELVVETYRLGSLATKDNVAESHALWNKYRDLMVMDLEANPTPPKVEFIRNLYIARLQIPHTTISDTFSEFSTFITRYDNANYESIMVSTNKLYAVALAKYNERSRYEDRLPKHGQVSLAEEWNAWAEYLEWETSRPKKKLDVDMACALYERCLLCFGEQARVWEEYVFFALEKSLGPRVKALLKRATRHCPWSGTLWAQYIIALERAYRPFEEVSEIKHRATTTGMLDLGGLEEVLKVNIAWCGFLKRRAFEHDAGEDDFDMAEMGISEAVTETGKKDPEYRLQRIHINFCTLAKKIDAAREIWKELAKTHGNSYEYWLRYYHWELSHGPGDKDYAGSALKAALGRNTLDWPEKILETWKNHVEDFGNVEDVEYVTVRYRKLSKEILERRAQEAQVAALQQQTVKDQQPEADTPQTDESARASSKRRRSVTEADDSQPKKKSKAAETLAPEQPAAHSPSPKSSVAKRDRENTTVIVKNLPPNYPEVRVRQFFRDCGTINSLKLIVEKDGKTSTATVEFESREDVLTAQTKNLKIVDGHSIEVQVGTGSTLYVTNFPPTADETCIRDLFKDCGEIVDIRFPSLKYNSHRRFCYVQFASSDEAQKATSLHGKQLGGKETLVAKISAPDQKHERSGAVYEGREVYIRNIDFQAHDNDVQELFQKYGRIEKVRLPPGPKKGTHKGYGFVTFSAKEEALAAVEGANNTQLKYRTLIISIADPNPSKQKTTNANPSTEKMRDSASDRNTDRPRQKSAYPAASQQPPAPAFSDIKQKTLGIMNLSDTVNDTKIRHMFEKYGPLRKVALRPDHQGAIVEYESVTDAGKATLALDGLELAGRKIKIGGLADLMRQSPERKVSKGFATNNGGGKVGVVAFKPSQVGAGGMKGQKRRAGLGFSGVVKKKDADGKKNEDIVMADGPPAREAPKTKSNADFKAMFLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPI6</accession>
    <name>D5GPI6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00011858001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430375">
        <property type="protein sequence ID" value="CAZ86429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842238.1">
        <property type="nucleotide sequence ID" value="XM_002842192.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPI6"/>
    <dbReference type="GeneID" id="9186669"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011858001"/>
    <dbReference type="eggNOG" id="ENOG502QRGR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032158_1_1_1"/>
    <dbReference type="InParanoid" id="D5GPI6"/>
    <dbReference type="OMA" id="GSMPVCH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015098">
        <property type="term" value="F:molybdate ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031563">
        <property type="entry name" value="MOT1/MOT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31970:SF9">
        <property type="entry name" value="MOLYBDATE TRANSPORTER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16983">
        <property type="entry name" value="MFS_MOT1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="156"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="180"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="228"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="259"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="299"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="330"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="394"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ86429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="449" mass="46993" checksum="79622E30E4978AF6" modified="2010-06-15" version="1">MALFASSFKYNLRTLRDNPLGEIAGSLGDLGTLLPLMTALAAGKSISLTATLIFSGAFNIYSGAFFGVPIVVQPMKAIASIALARQLSIKETMAAGIGVGIVVMLLSIIGMIGRLTDLIPLPIVKGIQVGAGLSLCLNAGSMLSGLGLNGSKWDDNLFWAISAFSVLYGFSRLPKFPFALLVFLLGGIFAAVEVATSGGSLPAFGFWWPLNPTIPTPDEFATGFGTAGVGQIALTILNSVIAVRYLCEDLMPTRPAPSVTALGISVGLMNLTGCWFGAMPVCHAGSGGLAAQHRFGARSGASVMLLGLVKVGAGLAFGESLASLLQRFPKSLLGIMVFAAGIELASVAENLNSSAKDLLCPASVAPGTEQARSAVYPPLRVLTDREKKERFLNMMVTAAMTLACKNTLVGFLAGYFFWALLRLQDRMESLEEARASYAPLNEASALLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPM6</accession>
    <name>D5GPM6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00011926001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0047 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430376">
        <property type="protein sequence ID" value="CAZ86469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842278.1">
        <property type="nucleotide sequence ID" value="XM_002842232.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPM6"/>
    <dbReference type="STRING" id="656061.D5GPM6"/>
    <dbReference type="GeneID" id="9186709"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011926001"/>
    <dbReference type="eggNOG" id="KOG3267">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096980_0_2_1"/>
    <dbReference type="InParanoid" id="D5GPM6"/>
    <dbReference type="OMA" id="TWQGIFF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.460">
        <property type="entry name" value="YjbQ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001602">
        <property type="entry name" value="UPF0047_YjbQ-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035917">
        <property type="entry name" value="YjbQ-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00149">
        <property type="entry name" value="TIGR00149_YjbQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30615">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN YJBQ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30615:SF8">
        <property type="entry name" value="UPF0047 PROTEIN C4A8.02C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01894">
        <property type="entry name" value="YjbQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004681">
        <property type="entry name" value="UCP004681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111038">
        <property type="entry name" value="YjbQ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ86469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="146" mass="16328" checksum="3FA87FCC747D577C" modified="2010-06-15" version="1">MPWFQKTLTLPPSSRGSYLITDTITSSLPELANYRVGLLTLFIQHTSCALSLNENWDSDVRADMSDALDRIVPEDSKREGIYRHDAEGTDDMPAHVKSSLVGVTVTVPIVEGRLGLGTWQGIWYLEFRRARHTRKVIATIQGGLKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>D5GQ13</accession>
    <name>D5GQ13_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00012129001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430380">
        <property type="protein sequence ID" value="CAZ86606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842415.1">
        <property type="nucleotide sequence ID" value="XM_002842369.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GQ13"/>
    <dbReference type="GeneID" id="9185184"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012129001"/>
    <dbReference type="eggNOG" id="ENOG502QSKG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010595_7_1_1"/>
    <dbReference type="InParanoid" id="D5GQ13"/>
    <dbReference type="OMA" id="WFEQAEI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591:SF24">
        <property type="entry name" value="2-METHOXY-6-POLYPRENYL-1,4-BENZOQUINOL METHYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13489">
        <property type="entry name" value="Methyltransf_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ86606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="345" mass="38665" checksum="7666C3CC1F160AEC" modified="2010-06-15" version="1">MAETQPDAPQIIEADDPGSGSDDESAYGSGVPSDTTSLASEVRAYVYENGRQYHAYSQGHYWAPNDGKQNEQLDIFHHVYSLVLDGNLHLAPIGEHPQKILDIGTGTGIWAIDMADAYPSAQIIGNDLSPIQPSWVPANCQFEVDDFTKPWTYSRDSFDFIHARGIYGSVGSWPILLREAYSRIKPGGWFESVETTVGYYHDDGPRGEGQLIPKDNPVYKWCRLGLEAAERIGKPFDVAGKVDGWLEDVGFINVTQKEYKVPTGAWPRDAHMKDIGKYNLLNMLEAIEGFTIALFTRVLGWSLMETQVILAQVRTELTDKRNHFYFKMYVAYGQKPEITEQEEGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>D5G437</accession>
    <name>D5G437_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00003934001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835159.1">
        <property type="nucleotide sequence ID" value="XM_002835113.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G437"/>
    <dbReference type="FunCoup" id="D5G437">
        <property type="interactions" value="35"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G437"/>
    <dbReference type="GeneID" id="9182407"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003934001"/>
    <dbReference type="eggNOG" id="KOG2953">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_403958_0_0_1"/>
    <dbReference type="InParanoid" id="D5G437"/>
    <dbReference type="OMA" id="QNNTHIS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006012">
        <property type="term" value="P:galactose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02642">
        <property type="entry name" value="R3H_encore_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.50">
        <property type="entry name" value="R3H-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001374">
        <property type="entry name" value="R3H_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036867">
        <property type="entry name" value="R3H_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051937">
        <property type="entry name" value="R3H_domain_containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024771">
        <property type="entry name" value="SUZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15672">
        <property type="entry name" value="CAMP-REGULATED PHOSPHOPROTEIN 21 RELATED R3H DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15672:SF8">
        <property type="entry name" value="PROTEIN ENCORE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01424">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12752">
        <property type="entry name" value="SUZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00393">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82708">
        <property type="entry name" value="R3H domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51061">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51673">
        <property type="entry name" value="SUZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="R3H" evidence="3">
        <location>
            <begin position="222"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="SUZ" evidence="4">
        <location>
            <begin position="286"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="113"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="288"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="484"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="15"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="60"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="76"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="307"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="355"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="388"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ79280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="681" mass="73122" checksum="066081F0E61ACE98" modified="2010-06-15" version="1">MSCPPSSASALAPVQPQNYSMVDSKLQDAQGSTTDSSIPATQACADIPTPSNIVGGITPSSSSSSTQLSDLEDSTQALAQAAKSSLPSTDGVTRLQESLSGLKLEKDILLSNGGSSSRLAVDTPPIALVPSNGDAPNTSLRGSTSHAEDTSSQPSHLVPSDGYNLLKRPVGSDGAETSPVIVVSSQKSASIPPPPSTHASVEMLIDEPDQRLLDALENQRDRLFVIKLEQDVIDFIEKNSSDVFDMPPINAYHRLLAHKMAEYYRLTHVADTSGTSVRLYRGQFARMPPVRLSDRRPPNAMPGPSAANGTKSNFKIMQRSTSHRGSDTPPEQQNGSFPKKTASESSSGDGSARATTREEREQAYQEARARIFKDFVESPPETPAPSKSNEKTRKQEKQDEFHGRSQFYPVPTPAPPVNPYCFPQYEDPRLQSQNNTHISGGSRFNPSTPSFNPSPFVPPLVSFTPPQQPQPQSFMPTLSNRHFPPQGDRSFPTPNQGHVGPNMFPQQQQRNGYYPEQGANFHSGNPQQSSLMMSTNFPNQRFGAQPPLHGQHSGPPMAASVPTTANRHPYASPSNYAVGNQALGNQRFHVPQQQQPLISGNGWSAPQTLNGGIPMTTRSQSALPALDRPTPPFGGMPYQNHQQSQHWGGNSVVGSSAPINFRQGPFGGAGGPGMHGPVGGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>D5G449</accession>
    <name>D5G449_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00003949001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835171.1">
        <property type="nucleotide sequence ID" value="XM_002835125.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G449"/>
    <dbReference type="STRING" id="656061.D5G449"/>
    <dbReference type="GeneID" id="9182419"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003949001"/>
    <dbReference type="eggNOG" id="KOG4488">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165034_2_0_1"/>
    <dbReference type="InParanoid" id="D5G449"/>
    <dbReference type="OMA" id="VTCRFRR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007513">
        <property type="entry name" value="SERF-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04419">
        <property type="entry name" value="SERF-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Small EDRK-rich factor-like N-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ79292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="83" mass="8546" checksum="066913D1F95CC9B1" modified="2010-06-15" version="1">MTRGNQRERDRAKAQKAAASQKSKTTKSKAEMQRDKDMVAQLMREKQKQAEEKKKAEASGAGSSSGSGSGGSGGGGGGGSSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4D2</accession>
    <name>D5G4D2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Palmitoyltransferase</fullName>
            <ecNumber evidence="11">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00004057001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DHHC palmitoyltransferase family. PFA5 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="11"/>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835254.1">
        <property type="nucleotide sequence ID" value="XM_002835208.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4D2"/>
    <dbReference type="STRING" id="656061.D5G4D2"/>
    <dbReference type="GeneID" id="9185000"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004057001"/>
    <dbReference type="eggNOG" id="KOG1315">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034009_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4D2"/>
    <dbReference type="OMA" id="SFYTKDV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF23">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="25"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="294"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="329"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="13">
        <location>
            <begin position="249"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="147"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="183"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ79375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="490" mass="54546" checksum="41D9110BFEB6A23E" modified="2010-06-15" version="1">MGNGWLKAREGRGPSKHALNRWVSYTMPVIMAGAVGFATWVFVMLVCVNHLLIGQGRKGTAIALLVVYFVLFLLMSSAYLRVLWVVSTSPGFVTKQSTDSENEKFLGSVESRSDAEIGLGGVQAEGWQREEERIQPPKLMAIAGGPRANETESLPDALQSRSTKGAPGPAFSPETIVQPTDGPSISSLPASSDPSLQSPAARTVFSENALGSRPYCTYGPPRPETGAGYQNLDEWFKRDAFVCENDGLPRWCVHCQLWKPDRSHHCSELQRCVWRMDHFCPWVGGVVAEPSYKFFYQMVFYGALYCLFLIVSIAVVFRQQINDNGRQDARWVACLALACVFGLFSGAMAGSTTQLVYRNVSTIDSLNYKTKVYQIALHDPNAAQIPPPGQKISAVMPARIWLPQEPGPGQRQRCFAIVSTRPGDNPWRLASTLENFQEVLGYNFWDWWLPIKRSPLEAKKSGEGWYRWNEELLSRLRHQAGITQPSGQSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4D5</accession>
    <name>D5G4D5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00004061001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4D5"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004061001"/>
    <dbReference type="HOGENOM" id="CLU_1856743_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4D5"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ79378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="138" mass="15318" checksum="02341F4448301AFC" modified="2010-06-15" version="1">MKPCRSRTSTHLRKSGGTGKCLYPWEGRGEWGFFRERGGWMDRCEQLFVLSHIGWVVSLDITRVGGLDQAKAYVPTTFALPPDDTTRLPQRLQVIPQIERVPAFIPRALGIGGTVPPLTLTCSCFAVQYYTGTVCLSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4F5</accession>
    <name>D5G4F5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00004086001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835277.1">
        <property type="nucleotide sequence ID" value="XM_002835231.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4F5"/>
    <dbReference type="GeneID" id="9185023"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004086001"/>
    <dbReference type="HOGENOM" id="CLU_2544249_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4F5"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ79398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="83" mass="9071" checksum="353910D219FA0A0B" modified="2010-06-15" version="1">MRNSSLAPFPTSRSFPDVSDIFPLPFRIPGYGLPYSGNGNSILWLLQALDGGECWRWGVVAGGGDGNEFARLNIIFQKKKIGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4W9</accession>
    <name>D5G4W9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00000126001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429992">
        <property type="protein sequence ID" value="CAZ79562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835405.1">
        <property type="nucleotide sequence ID" value="XM_002835359.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4W9"/>
    <dbReference type="FunCoup" id="D5G4W9">
        <property type="interactions" value="145"/>
    </dbReference>
    <dbReference type="GeneID" id="9183044"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000126001"/>
    <dbReference type="eggNOG" id="KOG4754">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039184_1_0_1"/>
    <dbReference type="InParanoid" id="D5G4W9"/>
    <dbReference type="OMA" id="NALPCDT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001345">
        <property type="entry name" value="PG/BPGM_mutase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050275">
        <property type="entry name" value="PGM_Phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48100">
        <property type="entry name" value="BROAD-SPECIFICITY PHOSPHATASE YOR283W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48100:SF54">
        <property type="entry name" value="PHOSPHATASE SPAC5H10.03-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00175">
        <property type="entry name" value="PG_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="216"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="229" mass="26163" checksum="F19F4F3628CC449A" modified="2010-06-15" version="1">MPPRIITLVRHGQAHHNIGWKFDWHDPYLTPLGETQCHELSERFPSEPPVDLLVSSPLRRTIQTTLLGFKQQIKSGVKMELLAELQESSEMPCDTGSSRDVLEKEELFRDVDFSGLPDDWTSKKGKWAPDPHSLGERARAVRKWLKSRSEGHVVVVLHGGFLHYIIEDWAGRSNLLGNCWRNTEFRSYVFADEDEDNASVVETDESKERRKGSKKPSGEGEAVEKVMRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4X2</accession>
    <name>D5G4X2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mannose-1-phosphate guanyltransferase</fullName>
            <ecNumber evidence="3">2.7.7.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">GDP-mannose pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">GTP-mannose-1-phosphate guanylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00000129001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in cell wall synthesis where it is required for glycosylation. Involved in cell cycle progression through cell-size checkpoint.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>alpha-D-mannose 1-phosphate + GTP + H(+) = GDP-alpha-D-mannose + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15229"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
            <dbReference type="EC" id="2.7.7.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; GDP-alpha-D-mannose from alpha-D-mannose 1-phosphate (GTP route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.13" evidence="3"/>
    <dbReference type="EMBL" id="FN429992">
        <property type="protein sequence ID" value="CAZ79565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835408.1">
        <property type="nucleotide sequence ID" value="XM_002835362.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4X2"/>
    <dbReference type="STRING" id="656061.D5G4X2"/>
    <dbReference type="GeneID" id="9183047"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000129001"/>
    <dbReference type="eggNOG" id="KOG1460">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_3_0_1"/>
    <dbReference type="InParanoid" id="D5G4X2"/>
    <dbReference type="OMA" id="MPVPNWW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004475">
        <property type="term" value="F:mannose-1-phosphate guanylyltransferase (GTP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd06428">
        <property type="entry name" value="M1P_guanylylT_A_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056729">
        <property type="entry name" value="GMPPB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050486">
        <property type="entry name" value="Mannose-1P_guanyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22572">
        <property type="entry name" value="SUGAR-1-PHOSPHATE GUANYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25087">
        <property type="entry name" value="GMPPB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="9">
        <location>
            <begin position="4"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="Mannose-1-phosphate guanyltransferase C-terminal" evidence="10">
        <location>
            <begin position="283"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF25087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ79565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="413" mass="45776" checksum="D38A5F99A59ECDB1" modified="2010-06-15" version="1">MSNKALILVGGPSRGTRFRPLSLDLPKPLFEVAGHPLIYHPLKAVAKVPSIKEVYLIGYYEESIFAPYIRQWSTEFPQFTIKYLREYTALGTAGGLYHFRDAIMKGNPSRLFVINADVCCSFPLKDMLDMFEEKEAEAVLLGTKVSNEAASNFGCIVVDPKTSQVRHYVEKPESHISNLINCGVYLFSSSVFSLIRSAMEDKAIRLASDPTLDPSDGDTSSVLRLEQDILGPLSDSRRFFVYETRDFWRQIKTAGSAVPANALYLQKAWQSESNELSQPSAFIVPPVYIHPTAVVDPTAKLGPNVSIGPRAVIGAGARVKESIVLEDAEIRHDACVLYSIIGWQSKVGAWARVEGTPMSVTEHSTTVVKNGVRVQSVSILSRDCKVGDEVRVWNCVTLPFKELKRDVSNEVIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>D5G510</accession>
    <name>D5G510_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00000304001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429995">
        <property type="protein sequence ID" value="CAZ79603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835446.1">
        <property type="nucleotide sequence ID" value="XM_002835400.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G510"/>
    <dbReference type="STRING" id="656061.D5G510"/>
    <dbReference type="GeneID" id="9186319"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000304001"/>
    <dbReference type="eggNOG" id="ENOG502S5SN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_436275_0_0_1"/>
    <dbReference type="InParanoid" id="D5G510"/>
    <dbReference type="OMA" id="WLTIYLT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052973">
        <property type="entry name" value="Fungal_sec-metab_reg_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35392:SF3">
        <property type="entry name" value="ZN(2)-C6 FUNGAL-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35392">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="153"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="627" mass="71593" checksum="955CA6AE27901E36" modified="2010-06-15" version="1">MLNSSKTCNNDMLFTPWPFDPHSGPAPHDLPGDDNQGLGDLLSASGVPNLKSEQYPAFMNSIFAPDLYFSSIDFAFPGDLTAGQANYIDERHFPCEYDGFANMDPSNLARLEDCITNVENNIDIYQELLRLDTTLPIFDQSAPWDQPGAWQPTSATSSCSSSSTLDASAGFHTPSSSPAGDHEESPDYQQNQRKNSSSESSDGEPRNNNSCKKKRQRKRFTPERKKETHMTRITGACLPCWKNKKRCIRVSEACCKYCESIFSKIPNIPCFRARMTESELFRRGPTEEFLWTRRRWLKASPNDRTVWASPAKGFTVTQGMGLTLNLMAKQYKPLPDDRDNYTWIGQNGEMAALACPPYAISDIDDAQRTINRYVDDNTVRYIQEHVSSNVILWESFSMALRMSNAKGSSLLRNALKLWVGSRIIETPWSITGNETFGMTTNMEEGSPYYGKIPVTPIMDFQIDNITIHQLLIPLRKKLLQELQRKVLNNKPEDFLEIYLTIFILLHNMELTIAHDRWFAMRYSLQNRFSNYHLIDAVFQGANILLAHFHHVSKGYAPFSIDWESGETIVLAKSNEEEIRYMKDVIQATKHQESGLKMLREKEIYEDSMYWCSQLFFPGWKPVNAPTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>D5G570</accession>
    <name>D5G570_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00000281001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429996">
        <property type="protein sequence ID" value="CAZ79655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835498.1">
        <property type="nucleotide sequence ID" value="XM_002835452.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G570"/>
    <dbReference type="GeneID" id="9186353"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000281001"/>
    <dbReference type="eggNOG" id="ENOG502RV3A">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026590_0_0_1"/>
    <dbReference type="InParanoid" id="D5G570"/>
    <dbReference type="OMA" id="ERTSWRT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14364">
        <property type="entry name" value="CUE_ASCC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052586">
        <property type="entry name" value="ASCC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041800">
        <property type="entry name" value="ASCC2_CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003892">
        <property type="entry name" value="CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21494:SF0">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1 COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21494">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1 COMPLEX SUBUNIT 2 ASC-1 COMPLEX SUBUNIT P100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02845">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00546">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51140">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CUE" evidence="2">
        <location>
            <begin position="275"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="320"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="428"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="466"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="530"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="467"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="538"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="589"/>
            <end position="608"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ79655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="608" mass="66743" checksum="F68DFD0E3EBA74EA" modified="2010-06-15" version="1">MLEIPIVPYPPPEIQSLIPPDKWAKYQDSCVTALTHHLLLPTPAFQNSLKDTHLIEFLTTYASNQAPTTQNPPLRRKVVQILHRIYTTASPKHVPGQLVTATFLTELSREFGKNRVIRELLDKEEVWGLFEKDVLKCGLEDAVSVVRFLPRVSESLLAGDEWMEGAIARGEVAALATALVAVASRQNWGFLGDVLYKLVAEGKRDGKTGEFVRKLIWETPVVRILEREVDGVPEEGRISGVLEEVRSFGPVRKKDKGKGREVLDLEGLSREEESSLTTKVESVRDILPHLGTGFVRRCLLQMDGDVERVTAAVLEGNLPGDLNLADQGEEFIPPEAPEPPSAYATPPSEPADSEFPALPVRRNIFDNDELDLLTPSATTRLHQGNKTNKLTADKLLDAPEVPKSAIYAALEAFDPDDDERDDTYDLADIGGTVDTSAPPGDGYSGDREGDSAENVLWDAYNSSPEVFNRDSETRRGKARKDLKDRTSMTDEAIEGWKVMLERDPNRLRAMERKFTDRGVLQNVIAKNSWRAGGDARGRGGGGRGARGGRGGGGVGRGRGSGSSGDVTGPANGKEMQQGRAKKEANKGSKANHNRRDQRAKKMARGFTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5I1</accession>
    <name>D5G5I1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00004345001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="9">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835617.1">
        <property type="nucleotide sequence ID" value="XM_002835571.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5I1"/>
    <dbReference type="STRING" id="656061.D5G5I1"/>
    <dbReference type="GeneID" id="9183760"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004345001"/>
    <dbReference type="eggNOG" id="KOG1035">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018993_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5I1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004694">
        <property type="term" value="F:eukaryotic translation initiation factor 2alpha kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050339">
        <property type="entry name" value="CC_SR_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054521">
        <property type="entry name" value="HRI2_3H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042:SF187">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22949">
        <property type="entry name" value="HRI2_3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0652">Protein synthesis inhibitor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="248"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="194"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="410"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="209"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ79774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="668" mass="72265" checksum="19039F412EC9BDE4" modified="2010-06-15" version="1">MFRKPSEISSSSEDEVDPSSDPPSTSSDPSRQPTAGTPEQALEPVNPVTLYSPIPPTTSANRHSAILMNSLIEHFVRSRVPASDPELADRVFAVIARHLGNLGFVPQTSGDLAREGLAPVRQGYIRGLDQILTQEIEKITEENGAARVLAGDLGRRGAESSNGAETRRTFRRLFRGTATQNLSPNDRQVIHRSSSLSVLSDARKNSSDTAPGAVAGTATPPFLPPHAQTTPATIQPTILLPSRYVNDFVEIAPLGRGGYGSVYHVRNRLDRVEYAVKKVVIKKSILENELGGKDGLERVMNEIKTLAQLNHVGIVRYFCSWIEGVIEDMLTASQFIGDHVGVQNAQGHRSPKAEVLSEIEDIGAEIVFAYSGENEGIETVEEATTIAGANGQASDDLEDSIELIPRSYLRRRRSQPDAQHGKPKVAFDISSHPDPGSEFSGPEVSGSSIFSGGGALIMSTANGHGKKPSSTSSGLTLYIQMSLHSFTLTSFISTSARPTTPRHCFCVKSSLDIFLGLLDGVQYLHSQGIAHRDLKPGNIFLDVEPSRRGCRCGEAVVRPHIGDFGLGTSIGKDGHDSTAAVGTKFYKPVDLDPAIQGDVVGRDVFALGVILLELLVKFGTRKLIQKPFYNVVLHLLTSLRYGTCGSLVQAFRRWSGSKGYRTQKPESR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5J8</accession>
    <name>D5G5J8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00004371001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835634.1">
        <property type="nucleotide sequence ID" value="XM_002835588.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5J8"/>
    <dbReference type="STRING" id="656061.D5G5J8"/>
    <dbReference type="GeneID" id="9183777"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004371001"/>
    <dbReference type="eggNOG" id="KOG1897">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002893_1_1_1"/>
    <dbReference type="InParanoid" id="D5G5J8"/>
    <dbReference type="OMA" id="ITTRIDI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018846">
        <property type="entry name" value="Beta-prop_RSE1/DDB1/CPSF1_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058543">
        <property type="entry name" value="Beta-prop_RSE1/DDB1/CPSF1_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050358">
        <property type="entry name" value="RSE1/DDB1/CFT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004871">
        <property type="entry name" value="RSE1/DDB1/CPSF1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10644">
        <property type="entry name" value="DNA REPAIR/RNA PROCESSING CPSF FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10433">
        <property type="entry name" value="Beta-prop_RSE1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23726">
        <property type="entry name" value="Beta-prop_RSE1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03178">
        <property type="entry name" value="CPSF_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RSE1/DDB1/CPSF1 first beta-propeller" evidence="4">
        <location>
            <begin position="12"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="RSE1/DDB1/CPSF1 second beta-propeller" evidence="5">
        <location>
            <begin position="422"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="domain" description="RSE1/DDB1/CPSF1 C-terminal" evidence="3">
        <location>
            <begin position="774"/>
            <end position="1045"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ79791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1053" mass="115302" checksum="F35D46C4BDD19C60" modified="2010-06-15" version="1">MSYLATIYEASSVRIAIKAEFLTVGETSLIVAKTSHIEVYKLDGPGIVLEDKFPIYGRVIALAAFRPVKSATDHLLIVIGKVNYQYFTISWDPVAKKPKTEHMAVNFSEYNAPLSDSFQCLADPGKNMLGIHVYKGIFLVIPQIQQSIKGSRRSRADLDVGNIGNPCVVRLKELEILDLKFLFGTISPVLAVLYKPSGADEMAVNTYELSVKSGEVKLLDWRIRDLKGGREALFLIPVRPPSNGLLLIGVTKIQYFDNYGNKTFLPVDPPMVWVTWEMLSPERYILGDEAGGLHMLTLSAGLMDTKVGLHLKLVGNASIPEILVHLNQGLLFLGSHSGDSQLLQLYQNGIKPMVRVQQVLRNIGPIVDFRVMDLDYSRSDEVMRQYSPGHIRFLSASGGHTQGHLRTIRSGVGLYDLGFLGEMSGIRGLWSLRSIPGSSFDDVLVVSFIEETRIFKFDNSGEIEELYEFMGFALNQRTILAHSVVGGRFLQVTATAVKLVDVRSNTLIAESYPDKSLTITIASANQDLLIYAMGPTLVLLNLARDLEEHIRTTFENEISCLNMPSSPSTICAVGFWTVSLVLILSVQSFSILSQEILSQEDSAATPRSLLFARLLENGPPTLLVALGDGSMFTFALNETTCGLSERKHIILGAQPIRFQSIPGGNGGVTVFATCDHPSVIYGSDGRIVYASVTADKPTYVTSFNSPSFPDAVVIASEDDLKLSVVDPVRTMHVQSLPVGDVVRRIAYSKEKNIIAIVTVSKVPDTRTGDYLYSSCIRLVDNTAFSVVDSYELNQLELVESLASGKICGGNGLLSEGFLAGTVYPGGGRDESEKGRIIVFNASETKRIKLIVSYDTPGSVNGIQIVGEGKFVAAIGREIHLYSLKYTDIQSKTAASESVGATGCTITKLASFKAHSTPLDVAVYNDIIAVCDFMHGPSILQHIEDKENKSSEFVEVARAPKPSWLTALELLDEKTVFCADTDGNLVVWQRQLSGVTEDDRKQLQQIASMKIGEDIDRIRRICNQTLAGSRVQPKAHLSTVDGAILLYGRHSKLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6N0</accession>
    <name>D5G6N0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Probable electron transfer flavoprotein subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00002136001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9 10">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="9 10">Binds 1 FAD per dimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 9">Heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the ETF alpha-subunit/FixB family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430012">
        <property type="protein sequence ID" value="CAZ80173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836016.1">
        <property type="nucleotide sequence ID" value="XM_002835970.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6N0"/>
    <dbReference type="FunCoup" id="D5G6N0">
        <property type="interactions" value="490"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G6N0"/>
    <dbReference type="GeneID" id="9187199"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002136001"/>
    <dbReference type="eggNOG" id="KOG3954">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034178_0_0_1"/>
    <dbReference type="InParanoid" id="D5G6N0"/>
    <dbReference type="OMA" id="WRPYAEQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01715">
        <property type="entry name" value="ETF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000041">
        <property type="entry name" value="Electron transfer flavoprotein alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1220:FF:000001">
        <property type="entry name" value="Electron transfer flavoprotein, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014730">
        <property type="entry name" value="ETF_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001308">
        <property type="entry name" value="ETF_a/FixB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033947">
        <property type="entry name" value="ETF_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014731">
        <property type="entry name" value="ETF_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018206">
        <property type="entry name" value="ETF_asu_C_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43153">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43153:SF1">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01012">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00766">
        <property type="entry name" value="ETF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000089">
        <property type="entry name" value="Electra_flavoP_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00893">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00696">
        <property type="entry name" value="ETF_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6 9 10" id="KW-0274">FAD</keyword>
    <keyword evidence="5 9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Electron transfer flavoprotein alpha/beta-subunit N-terminal" evidence="11">
        <location>
            <begin position="38"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="266"/>
            <end position="267"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="280"/>
            <end position="284"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="297"/>
            <end position="304"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="318"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000089-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ80173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="353" mass="37377" checksum="BC3FF324DBC61296" modified="2010-06-15" version="1">MSICVAQTRASLLRLPHQSARSTVRSRPFTPSPRLLNTLAVLEHKDGKVSSQSFPVVTAASRFGGPVTVFLAGSGSKEVSEQAAKLNGVEKVIYASNSAYDKGLPENFAPLLVENIKRGHFTHVVGSHSAFGKNILPRASALLDIQQISDCIEIKDENTFVRPIYAGNAIATVRSTDTIKIVTIRGTAFPPAEETSDTATIEEAPIDPNAECTTVWVSENLTRSERPDLGTAPKVVSGGRGLKDKKNFDRLMLPLADALGAAIGASRAAVDSGFADNSLQVGQTGKVVAPQLYLAAGISGAIQHLAGMKDSKVIAAINKDPDAPIFQVADVGLVADLFQAVPELTEKLNAEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6Q6</accession>
    <name>D5G6Q6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="13">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00002171001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430012">
        <property type="protein sequence ID" value="CAZ80199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836042.1">
        <property type="nucleotide sequence ID" value="XM_002835996.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6Q6"/>
    <dbReference type="STRING" id="656061.D5G6Q6"/>
    <dbReference type="GeneID" id="9187225"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002171001"/>
    <dbReference type="eggNOG" id="KOG1601">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_494482_0_0_1"/>
    <dbReference type="InParanoid" id="D5G6Q6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000007">
        <property type="entry name" value="Nitrogen regulatory AreA, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056998">
        <property type="entry name" value="Asd-4/GZF3_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039355">
        <property type="entry name" value="Transcription_factor_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071:SF338">
        <property type="entry name" value="GATA-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071">
        <property type="entry name" value="TRANSCRIPTION FACTOR GATA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25026">
        <property type="entry name" value="Asd-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00619">
        <property type="entry name" value="GATAZNFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="GATA-type" evidence="12">
        <location>
            <begin position="288"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="334"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="418"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="486"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="352"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="379"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="418"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ80199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="551" mass="60789" checksum="0A6A7F58BA699EF7" modified="2010-06-15" version="1">MIVIWHPPPQSTRERRVDESVGVILFFPPSFSFLLWCVYLHLSKKHFFSHRSGLIYCLRTTPFPLFRCFFPPSILIPCSPRSVTALHKKKPSPPPLTYPSSFHSFPPRAPSYIPHPPSPAPAIHQHSLSFFHYRNIALVFYSHPHILLVRFYLPTTFELIRLPASPHPLPHSKTKPLPNIPSSHLHTLPPHIINNHKLAPTALFYSPAPERLPSPHHLLHPTSGSDETILPSISRTPSRSPLPSLNNTLASVALPSPPLRINGGFPMTFATSSPGASTAGQGTTPPICQNCTTSTTPLWRRDESGSVLCNACGLFLKLHGKARPISLKTDVIKSRNRVKNSGQPAKKKSLFDSPNGSANNHSAARSENGTPPPGHRKPSSGTASNRSVSPQSRAGTPLPPPSHMAPSIFESLHQAENSMVGSPSTLPAINTLNHPSPGSTSSLHDRHLENPALTLDCLISANNKLKTRVAELELVNDLFRGRVSELETSEANARRAEITRREVEAQLRLCLDASAKREEELKRKLDDAERELTDLRGDQQRKKVRVSDLTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6Q8</accession>
    <name>D5G6Q8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">tRNA(His) guanylyltransferase</fullName>
            <ecNumber evidence="3 14">2.7.7.79</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 14">tRNA-histidine guanylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">GSTUM_00002202001</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 14">Adds a GMP to the 5'-end of tRNA(His) after transcription and RNase P cleavage.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>a 5'-end ribonucleotide-tRNA(His) + GTP + ATP + H2O = a 5'-end phospho-guanosine-ribonucleotide-tRNA(His) + AMP + 2 diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54564"/>
            <dbReference type="Rhea" id="RHEA-COMP:14193"/>
            <dbReference type="Rhea" id="RHEA-COMP:14917"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:141847"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.7.79"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="16">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the tRNA(His) guanylyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.79" evidence="3 14"/>
    <dbReference type="EMBL" id="FN430013">
        <property type="protein sequence ID" value="CAZ80201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836044.1">
        <property type="nucleotide sequence ID" value="XM_002835998.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6Q8"/>
    <dbReference type="FunCoup" id="D5G6Q8">
        <property type="interactions" value="719"/>
    </dbReference>
    <dbReference type="GeneID" id="9187228"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002202001"/>
    <dbReference type="eggNOG" id="KOG2721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044271_1_0_1"/>
    <dbReference type="InParanoid" id="D5G6Q8"/>
    <dbReference type="OMA" id="VYFWSTH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008193">
        <property type="term" value="F:tRNA guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3000:FF:000001">
        <property type="entry name" value="tRNA(His) guanylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025845">
        <property type="entry name" value="Thg1_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024956">
        <property type="entry name" value="tRNAHis_GuaTrfase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007537">
        <property type="entry name" value="tRNAHis_GuaTrfase_Thg1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038469">
        <property type="entry name" value="tRNAHis_GuaTrfase_Thg1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12729">
        <property type="entry name" value="TRNA(HIS) GUANYLYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12729:SF6">
        <property type="entry name" value="TRNA(HIS) GUANYLYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04446">
        <property type="entry name" value="Thg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14413">
        <property type="entry name" value="Thg1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028980">
        <property type="entry name" value="tRNAHis_guanylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14 15" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10 14 16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 14 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 14" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 14" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNAHis guanylyltransferase catalytic" evidence="18">
        <location>
            <begin position="6"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Thg1 C-terminal" evidence="19">
        <location>
            <begin position="138"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="208"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="75"/>
            <end position="76"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR028980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR028980-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR028980-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF14413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CAZ80201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="269" mass="31198" checksum="4457FBD01B6479F7" modified="2010-06-15" version="1">MANSKYDYVRGFEAPHHLLRDTYIILRLDGRCFTKFAASHHFQKPNDPRALHLMNASASATMRFIPEIALAFGQSDGFSFLLPRECALFDRREEKLLSIVVFLWPQYFAGETGGEEQRPFMAPPSFDCRATCYPHVENVKHYFSWRQVDTYNNNLYNTTFWALVLKGGLKRHEAAEELQGTSSADKNEILFSRFGINYNNEPAIYRKGSVQQKQQEEEHETAGIPKFKSKTPRKKEEKRTRKTKIVIDHVDIIGEGFWERRPWLLGSGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6W7</accession>
    <name>D5G6W7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00002221001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430018">
        <property type="protein sequence ID" value="CAZ80260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842525.1">
        <property type="nucleotide sequence ID" value="XM_002842479.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6W7"/>
    <dbReference type="STRING" id="656061.D5G6W7"/>
    <dbReference type="GeneID" id="9184075"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002221001"/>
    <dbReference type="eggNOG" id="KOG0102">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2575583_0_0_1"/>
    <dbReference type="InParanoid" id="D5G6W7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ80260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="81" mass="8990" checksum="DCF105476E6FBEFC" modified="2010-06-15" version="1">MTRSQFETLANDLIQKCIDPCKKALKDAGLKPTDIQEVILVGGMTRMPKVVETVKVLDSDEVVTIIQINFGKDTSSCYLIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5G719</accession>
    <name>D5G719_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00004562001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430020">
        <property type="protein sequence ID" value="CAZ80312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836121.1">
        <property type="nucleotide sequence ID" value="XM_002836075.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G719"/>
    <dbReference type="STRING" id="656061.D5G719"/>
    <dbReference type="GeneID" id="9182503"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004562001"/>
    <dbReference type="eggNOG" id="KOG0895">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_270763_0_0_1"/>
    <dbReference type="InParanoid" id="D5G719"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004869">
        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23810">
        <property type="entry name" value="UBCc_BIRC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46116">
        <property type="entry name" value="(E3-INDEPENDENT) E2 UBIQUITIN-CONJUGATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46116:SF39">
        <property type="entry name" value="BACULOVIRAL IAP REPEAT-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="4">
        <location>
            <begin position="963"/>
            <end position="1122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="120"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="211"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="297"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="397"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="552"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="896"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="9"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="133"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="162"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="312"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="413"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="896"/>
            <end position="913"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ80312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1201" mass="131009" checksum="84C6A2B357CAA1F9" modified="2010-06-15" version="1">MTQPQKRASSSFSSGPSSRISSEITSPEDTGNPSEVDVLNLRNWWTALHPKGRCCSQCNNELHFESVTTDTLRNQLDSIYRIKNAVRNAEKVEKHRKEIEEKAREETISRLAKWGRVKRLASGNGPSMSKTGLEIDKENIEDEGKEKEKEKEKENGNAQDGKSGKDAKGKGKEKEDKPNAKKISQIFGTECCPKCRTYLCIACLKPAKPESDQKWEDLNGANPDGNDTSPRTSERSFRDDKRLWCCSDGKLVAILLCLGIIDEEFLGLSANGNSLSSASAGRGIKGTLRRSLRSLKDKITDGPHPAKPLVSKPERKTRTKGTKDKGTGYGSGAYTASMFGFDTSDSMDWDFPEDESDFDDGPLMFSDLEFPVTDSGIGKLSEKIAAQVAALSGPPLASTKPLGVTHNSQAQPEPTPAPKIKPPPGLSTPEGLPLASATPLFFPNQPFQPPPGPSTVEAVPLTSPLLQLPSEYQFKPSRGFPIANGLPLKAPPPYSPSNTASNEPANPKAPPMPMVYPYHGFGLGRGATPLSGLPYSLPHAPPHPAVKTPMAPGHQYHPSFFQGPSKPLTNSPGGPSSAKDPLPNSIEGIPGLPIFQNATGPPLSASDMMQHLKSYVAQHPEAGLSPLGYIPAYIPASSDPDHANTLLEAVGAPSGFADDGIVVPHLWPGPPEAFHSRSTLKPQVMPTHPPLPSKQIMHDKMLYAVVRTLIRLLSDSGSENELQYAMPDVTLSMLRASMLAEKMEELLRNDSIQDISIHSEVYLALVALLNRLSDNPSLEFLILENRRSKLFTSGIMKITQSKPAIDLLAEIGPKTRGKAAAAVEERKRAEVLMYEDGFSRHSMVQVMSKLSTQAKVFLNCVGKTKGDEFEGTEAAKTLKICMEIAAIGEKLLKLSSRNASNQNRSSSISSKSIEAPPQITSPPAAPVLDLGYHDGMAFEYVEGVVTTHYWNSQATSLTTSPRGRVLHLSREMATMSTSLPQGVFVRVEDGRPDIIKAMIVGPCETPYEGGLYEFDIWAPANYPSGPPMCQFKTTGNGTVHFNPNLYNCGKVCLSILNTWSGASSERWQPGTSTLLQVFVSIQSMILCAAPWYNEPGRREGTRKKDAEEFNSRVRVNSVKWAMLDWLKDSSKRNGIWKDVIEAHFKANQGYLLELVKEWAKKDPRLTNSGANVGTNKWNSYEDTSGIAQNYALALEEEFSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7B3</accession>
    <name>D5G7B3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00002425001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IFRD family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430023">
        <property type="protein sequence ID" value="CAZ80406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836215.1">
        <property type="nucleotide sequence ID" value="XM_002836169.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7B3"/>
    <dbReference type="STRING" id="656061.D5G7B3"/>
    <dbReference type="GeneID" id="9185304"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002425001"/>
    <dbReference type="eggNOG" id="KOG2842">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039188_0_0_1"/>
    <dbReference type="InParanoid" id="D5G7B3"/>
    <dbReference type="OMA" id="HISGRHI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039777">
        <property type="entry name" value="IFRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007701">
        <property type="entry name" value="Interferon-rel_develop_reg_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12354">
        <property type="entry name" value="INTERFERON-RELATED DEVELOPMENTAL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12354:SF1">
        <property type="entry name" value="INTERFERON-RELATED DEVELOPMENTAL REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05004">
        <property type="entry name" value="IFRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Interferon-related developmental regulator N-terminal" evidence="3">
        <location>
            <begin position="69"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="21"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ80406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="437" mass="48532" checksum="3DADE5BD5F285B5D" modified="2010-06-15" version="1">MSELRRKALGSGKTVSRKAASRQSSAASSRANSRNTSRANSRAASRANTDDEGSLSDETTFSLGSVDELATIDAELDETTTIWQEELGDRINQITERKKSSSVGREESLTAYVRILCAKYAKEEILSKKSELLDAFVKSLKQGKTEKEALLAAKALAITIVTDPEDTLFDKLAPDFKRAIRDHASLPLKNALIHALGAVVFYGGASVAETELIMDFFMEIVESDGHDIGAGDDAGIVAAALEEWGNLATQLDDAEEVTRRSMSSLVDQLDSSEILVQIAAGENIALLYEKSYAEGEDDEVVEDHSESGLGKNFVQKYKPYPRHGDLLHTLRSLSSGSKKYLSKRNKKTQHSAFVDILHSVENPMKGPRYSEALDKDNRVRGSRMTVRIHKKGLLRVERWWKLHRLQHLRRILAGGFLNHWIENPVIFESLSLFVEEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7M1</accession>
    <name>D5G7M1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00004620001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430031">
        <property type="protein sequence ID" value="CAZ80514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836323.1">
        <property type="nucleotide sequence ID" value="XM_002836277.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7M1"/>
    <dbReference type="FunCoup" id="D5G7M1">
        <property type="interactions" value="1416"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G7M1"/>
    <dbReference type="GeneID" id="9186993"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004620001"/>
    <dbReference type="eggNOG" id="KOG0157">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_27_0_1"/>
    <dbReference type="InParanoid" id="D5G7M1"/>
    <dbReference type="OMA" id="HEITNCE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11063">
        <property type="entry name" value="CYP52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047146">
        <property type="entry name" value="Cyt_P450_E_CYP52_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24287">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24287:SF1">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 9" id="KW-0349">Heme</keyword>
    <keyword evidence="6 8 9" id="KW-0408">Iron</keyword>
    <keyword evidence="4 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="8">
        <location>
            <position position="335"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ80514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="388" mass="43715" checksum="8DDF8C2E25ED653B" modified="2010-06-15" version="1">MGIFAVDGVSWSHSRALLRPSFDRTNVSDLGQLEEFMKIFFSRIDELPIQSFAHARAVELQELFQCLTMDSSTNFLFGNPIGALASRKYTLGNGEMTFDQAFDIAQYGLAMRAPLSSFYWLYNGAEFRKACSTARTQAALYVSRTLRKLNLAEKSEGKKYVFLEELAERTQDPQVLQDQVLSVMLAGRDTTAALLSWTFLCLAKYPEVFKKLRAEISEVVGVDEDARLPTQAELRGMVYLRWIIQEVLRLYPPVPINARRALRATTLPYGGGPDGNSPISIRKGERIVYSAFSLHRRTDIYGPDAGEFRPERWGEEALRGIGWSWLPFNGGPRICLGQQMALTHASYFITRIMQVYKEITPQDSALEGDAYDTKLVMASGRGVHVFLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7N9</accession>
    <name>D5G7N9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Citrate synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00004657001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>oxaloacetate + acetyl-CoA + H2O = citrate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16845"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16947"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>propanoyl-CoA + oxaloacetate + H2O = (2S,3S)-2-methylcitrate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23780"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57392"/>
            <dbReference type="ChEBI" id="CHEBI:58853"/>
            <dbReference type="EC" id="2.3.3.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Organic acid metabolism; propanoate degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the citrate synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430031">
        <property type="protein sequence ID" value="CAZ80532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836341.1">
        <property type="nucleotide sequence ID" value="XM_002836295.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7N9"/>
    <dbReference type="STRING" id="656061.D5G7N9"/>
    <dbReference type="GeneID" id="9187011"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004657001"/>
    <dbReference type="eggNOG" id="KOG2617">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022049_2_1_1"/>
    <dbReference type="InParanoid" id="D5G7N9"/>
    <dbReference type="OMA" id="IDHGFNA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050440">
        <property type="term" value="F:2-methylcitrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036440">
        <property type="term" value="F:citrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006101">
        <property type="term" value="P:citrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019629">
        <property type="term" value="P:propionate catabolic process, 2-methylcitrate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.580.10:FF:000001">
        <property type="entry name" value="Citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.580.10">
        <property type="entry name" value="Citrate Synthase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.230.10">
        <property type="entry name" value="Cytochrome P450-Terp, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016142">
        <property type="entry name" value="Citrate_synth-like_lrg_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016143">
        <property type="entry name" value="Citrate_synth-like_sm_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002020">
        <property type="entry name" value="Citrate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019810">
        <property type="entry name" value="Citrate_synthase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010109">
        <property type="entry name" value="Citrate_synthase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036969">
        <property type="entry name" value="Citrate_synthase_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01793">
        <property type="entry name" value="cit_synth_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007128">
        <property type="entry name" value="PRK09569.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11739">
        <property type="entry name" value="CITRATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11739:SF15">
        <property type="entry name" value="CITRATE SYNTHASE 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00285">
        <property type="entry name" value="Citrate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00143">
        <property type="entry name" value="CITRTSNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48256">
        <property type="entry name" value="Citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00480">
        <property type="entry name" value="CITRATE_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="active site" evidence="9">
        <location>
            <position position="304"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="350"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR610109-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ80532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="468" mass="50872" checksum="2DF3F9CC4BD1155D" modified="2010-06-15" version="1">MSTRALSCNLGGIASRIRLVYAGQRNASTLAQKDLKDTLKEIIPAKREALKRLKAEHGQKVLGEVKVENAIGGMRGIKGMLWEGSVLDANEGIRFHGKTIKDCQKVLPKGTFGEEMLPEGMFWLLLTGQVPSVEQVRGLSRTLAENAGLPDFVGLMLDNMPTTLHPMTQFAIAVSALNHNSKFAKLYEKGLSKADYWEPTFDDSISLLAKLPTIAAKIYQNTYLGGGPLPSPVATQDWSYNYAAMLGKSDNTGFVDLLRLYLALHGDHEGGNVSAHATHLVGSALSDPFLSYSAGLQGLAGPLHGLAAQEVLRFIQAMRKVVGDTYSDKDIENYLWSLLNSGQVIPGYGHAVLRKPDPRFEALMGFAESRPEVAEGAVFKLVKKNSEIAPRVLTEHGKTKNPHPNVDSASGVLFHHYGINQDLYYTVTFGVSRGLGPLAQLIWDRALGLPIERPKSLSMEAISKAIGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5G824</accession>
    <name>D5G824_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00002722001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430038">
        <property type="protein sequence ID" value="CAZ80667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836476.1">
        <property type="nucleotide sequence ID" value="XM_002836430.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G824"/>
    <dbReference type="GeneID" id="9184139"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002722001"/>
    <dbReference type="HOGENOM" id="CLU_3351396_0_0_1"/>
    <dbReference type="InParanoid" id="D5G824"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ80667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="37" mass="4038" checksum="7B7E0154749CC8B2" modified="2010-06-15" version="1" fragment="single">MPPQTPLSATSPIQSLYPTLIRPIIAPDFYQPLLADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8A6</accession>
    <name>D5G8A6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="12">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00002953001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NADH dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430041">
        <property type="protein sequence ID" value="CAZ80749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836558.1">
        <property type="nucleotide sequence ID" value="XM_002836512.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8A6"/>
    <dbReference type="STRING" id="656061.D5G8A6"/>
    <dbReference type="GeneID" id="9188351"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002953001"/>
    <dbReference type="eggNOG" id="KOG2495">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021377_1_1_1"/>
    <dbReference type="InParanoid" id="D5G8A6"/>
    <dbReference type="OMA" id="HVDVLTN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003954">
        <property type="term" value="F:NADH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.100:FF:000005">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase 64 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045024">
        <property type="entry name" value="NDH-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054585">
        <property type="entry name" value="NDH2-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43706">
        <property type="entry name" value="NADH DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43706:SF50">
        <property type="entry name" value="NADH DEHYDROGENASE (UBIQUINONE)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00036">
        <property type="entry name" value="EF-hand_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22366">
        <property type="entry name" value="NDH2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="85"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="11">
        <location>
            <begin position="533"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="35"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="35"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="62"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ80749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="691" mass="77161" checksum="279FD38318FC3356" modified="2010-06-15" version="1">MLRQRVLSSFHTNPLTRQIFKPSPLLRTLQKLNTRRASPSPLSNASLRTIPARSSPLPTPHSRASATIAPSNGSKTPKRSKVVRFVYKASAFCGFAGLTATGLVVAFFIYDASTYKEEAEKYDIGVSELALNPRRGGPKNLPIAEVLVDDEDGEEMLKQRDKPRLVVLGCGWGSVSLLKNINPDNYHITVVSPSNYFLYTPLLPSATVGTLELRSLVEPIRRITSRVKGHFLKANAEGVDFSAKLVEVSQTLPSGEVRRFYLPYDKLVIGVGSKTRTHGVEGLENVEFLKNVADARKIRSKVIECFERACLPSTTDEERRKLLSFVICGGGPTGVEFAAELFDMLNEDLTLVYPKILRNEVSVHVVQSRSHILNTYDEALSRYAEERFARDQVDVLTNARVSRIENDRVIFTQKEKGSGKVITKELPFGMCLWSTGVAQTDFAEHIASQLELQRNKHALETDSHLRLLGTPLGDVYAIGDCSTVQNNIASHITHFLRQIAWEKGADPEKLALDFGMWRNVATRVRKKFPQATDHLRRLDRLFEQYDVDKSGTLDFDELKELLSQIDSKLTSLPATAQRAHQQGQYLARKFNKLAQAAPGLAVNNVTDGDLDEAVYKGFEYKHFGSLAYIGNAAVFDLNGLSIGGGLIFVYLWRSVYFAQSVSLRTRMLLAMDWGKRALFGRGEIKFPFLCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8G8</accession>
    <name>D5G8G8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00002858001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430043">
        <property type="protein sequence ID" value="CAZ80811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836620.1">
        <property type="nucleotide sequence ID" value="XM_002836574.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8G8"/>
    <dbReference type="STRING" id="656061.D5G8G8"/>
    <dbReference type="GeneID" id="9186030"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002858001"/>
    <dbReference type="eggNOG" id="KOG1209">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_9_1"/>
    <dbReference type="InParanoid" id="D5G8G8"/>
    <dbReference type="OMA" id="ETHVFGP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05374">
        <property type="entry name" value="17beta-HSD-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051911">
        <property type="entry name" value="SDR_oxidoreductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43976">
        <property type="entry name" value="SHORT CHAIN DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43976:SF16">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE_REDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ80811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="276" mass="28914" checksum="6EFFECAE4EAAFF1E" modified="2010-06-15" version="1">MAKTWLITGASSGIGREISLAALARGDTVIATSRTPTRLADLSTRGATTLKLDLTSPHSELASVVAQALAQHENIDILVNVAGYLLEGAVEETDEGETDRLYKTNLFGPMNLARALLPHFRARSSGVIANVAGIGALRGAAAAGYFCGSKAALRVVTEALRAEVAAFGIEVCCVMLGHFRTAFLEHGHRLVAQERIEEYEGVLGGIRRGFSAFHGHQPGDPVKASAVIVRVLAGDAGLGGVPGVLPVGGDVFEKMGEVEGLREVAGYTNYQGEEGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8H4</accession>
    <name>D5G8H4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00002874001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430046">
        <property type="protein sequence ID" value="CAZ80821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836630.1">
        <property type="nucleotide sequence ID" value="XM_002836584.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8H4"/>
    <dbReference type="GeneID" id="9186041"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002874001"/>
    <dbReference type="eggNOG" id="KOG4569">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032957_1_0_1"/>
    <dbReference type="InParanoid" id="D5G8H4"/>
    <dbReference type="OMA" id="HRWYTIY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00519">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051299">
        <property type="entry name" value="AB_hydrolase_lip/est"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002921">
        <property type="entry name" value="Fungal_lipase-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46640:SF1">
        <property type="entry name" value="FUNGAL LIPASE-LIKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46640">
        <property type="entry name" value="TRIACYLGLYCEROL LIPASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G06510)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01764">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072586" evidence="3">
        <location>
            <begin position="20"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="Fungal lipase-type" evidence="4">
        <location>
            <begin position="136"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ80821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="336" mass="36563" checksum="59B5EF76F6FD06DF" modified="2010-06-15" version="1">MLPSAILTLLAVFAAGVVGSPTGIPQDDVDRDRTIEELLAMDADLVENDIETFSPKASARTISESELATVYYYAEFAAAAYCDPDHVPGTEASCKNNICPQVTANSVTTIIEFADTTSSDTTGIVARDDTRRTITVSYRGTQSLGNWFANVQIRWSDASVYCSSCKLHTGFYNAFRDAFPPILASINSLRAQYPSYKLVVTGHSFGGALATITATEFRRLGYTTELYTYGAPRVGNDKFCLFVSQSSGNYRVTHLNDPVPRLPPVALGYYHISTEYWISRGDTNIKAGDVEIYEGILNTGGNTGQSFIPPNITAHPGYFLPVGISGCGSSWDFDFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8L0</accession>
    <name>D5G8L0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 11">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="7 11">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00002995001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10 11">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7 10 11">Belongs to the peptidase C12 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="7 11"/>
    <dbReference type="EMBL" id="FN430047">
        <property type="protein sequence ID" value="CAZ80853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836662.1">
        <property type="nucleotide sequence ID" value="XM_002836616.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8L0"/>
    <dbReference type="STRING" id="656061.D5G8L0"/>
    <dbReference type="MEROPS" id="C12.009"/>
    <dbReference type="GeneID" id="9186221"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002995001"/>
    <dbReference type="eggNOG" id="KOG2778">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018316_1_0_1"/>
    <dbReference type="InParanoid" id="D5G8L0"/>
    <dbReference type="OMA" id="YIQYEIQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019784">
        <property type="term" value="F:deNEDDylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071629">
        <property type="term" value="P:cytoplasm protein quality control by the ubiquitin-proteasome system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000338">
        <property type="term" value="P:protein deneddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09617">
        <property type="entry name" value="Peptidase_C12_UCH37_BAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.532.10:FF:000009">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.532.10">
        <property type="entry name" value="Peptidase C12, ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001578">
        <property type="entry name" value="Peptidase_C12_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036959">
        <property type="entry name" value="Peptidase_C12_UCH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017390">
        <property type="entry name" value="Ubiquitinyl_hydrolase_UCH37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041507">
        <property type="entry name" value="UCH_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10589">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10589:SF16">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE ISOZYME L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01088">
        <property type="entry name" value="Peptidase_C12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18031">
        <property type="entry name" value="UCH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038120">
        <property type="entry name" value="Ubiquitinyl_hydrolase_UCH37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00707">
        <property type="entry name" value="UBCTHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52048">
        <property type="entry name" value="UCH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7 10" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7 10" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4 7 10" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UCH catalytic" evidence="13">
        <location>
            <begin position="4"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="291"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8 10">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8 10">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="10">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <feature type="site" description="Important for enzyme activity" evidence="9 10">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR038120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR038120-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR038120-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS52048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ80853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="313" mass="35679" checksum="493E059C12BDBC5A" modified="2010-06-15" version="1">MSGGWNTIESDAGVFTYLVDNLGVKNVQFEEMIALDADYLNQQSPVYGVIFLFKYPVGQKKADEAVDGQYDFAASENIFFANQTIQNACATQAILSVLLNRGDLDLGDQLREFKEFTQAFPAELRGEALSNSELIRGVHNSFARSSPFIDEGTRAATEDDDLYHFIAYTPINGILYEIDGLQPSPISHGKCTEEQFPEKVIPVLQRRIERYPATEIRFNLLAMVQDLRIRAAEIGDVETVAREDEKRKMWIWENELRRHNFVGFAHEVLKRAVASKLRDGKYDEWIDEAKQKAKDRMSERKKVRGEKEDIASP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8U5</accession>
    <name>D5G8U5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pre-mRNA-splicing factor CWC24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00004845001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in pre-mRNA splicing.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Associated with the spliceosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the CWC24 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430053">
        <property type="protein sequence ID" value="CAZ80938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836747.1">
        <property type="nucleotide sequence ID" value="XM_002836701.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8U5"/>
    <dbReference type="STRING" id="656061.D5G8U5"/>
    <dbReference type="GeneID" id="9186447"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004845001"/>
    <dbReference type="eggNOG" id="KOG1813">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050460_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8U5"/>
    <dbReference type="OMA" id="ANFRKKP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005684">
        <property type="term" value="C:U2-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034247">
        <property type="term" value="P:snoRNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16539">
        <property type="entry name" value="RING-HC_RNF113A_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039971">
        <property type="entry name" value="CWC24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12930:SF0">
        <property type="entry name" value="RING FINGER PROTEIN 113B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12930">
        <property type="entry name" value="ZINC FINGER PROTEIN 183"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="4 5 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="10">
        <location>
            <begin position="183"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="257"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="183"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="228"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="302"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="25"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="44"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="73"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="100"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="228"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ80938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="336" mass="37537" checksum="6A3FC0F5FAF239E6" modified="2010-06-15" version="1">MTEPVPQSDTPATTVPAPTVTFFKRSTKNRSSIRKRPATPPPTTTSDSSDAYSSSDEKATGTRCVKKRRKNKSVVTTKTTSKPSSATLGNSEDVKRRYTDVSSELTNANDATKTNWHNHGDNETSSLSSKKNTAEGEGEPIVGGAYKGTSSYTSFIKKSENAPARSVGPMKAPSNIRTITITDYAPDVCKDYKQTGFCGFGDTCKFLHAREDYAQGWKLDRDWEIDQKTGKAKNKKGDDSKGEDEEERELKDIPFKCVICKDDYKSPIVTKCKHYFCETCAIARYKKTPNCEICGKRTDGAFSMAKELRKKLERKVVREEERRKKEEAEAMMMESN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9B5</accession>
    <name>D5G9B5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00003297001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430058">
        <property type="protein sequence ID" value="CAZ81108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836917.1">
        <property type="nucleotide sequence ID" value="XM_002836871.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9B5"/>
    <dbReference type="GeneID" id="9182859"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003297001"/>
    <dbReference type="HOGENOM" id="CLU_1929122_0_0_1"/>
    <dbReference type="InParanoid" id="D5G9B5"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ81108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="131" mass="13768" checksum="E763CCFE57CB7BFB" modified="2010-06-15" version="1">MTLHCSSLPAIPTTRLTPRTFFAICTTMEPIAAAAAETTMVLSEKEEAAPTLSRPKYAVSAVPPMAMKSAGLVFGGIFTSCQSLGESVSTRTYSLQPKAPRTRSPFLCSGWELSITSPIAVALITSPSLTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9K2</accession>
    <name>D5G9K2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">4-nitrophenylphosphatase</fullName>
            <shortName evidence="5">PNPPase</shortName>
            <ecNumber evidence="3 5">3.1.3.41</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00003360001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2 5">
            <text>4-nitrophenyl phosphate + H2O = 4-nitrophenol + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21664"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57917"/>
            <dbReference type="ChEBI" id="CHEBI:61146"/>
            <dbReference type="EC" id="3.1.3.41"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Divalent metal ions. Mg(2+) is the most effective.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.41" evidence="3 5"/>
    <dbReference type="EMBL" id="FN430062">
        <property type="protein sequence ID" value="CAZ81195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837004.1">
        <property type="nucleotide sequence ID" value="XM_002836958.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9K2"/>
    <dbReference type="FunCoup" id="D5G9K2">
        <property type="interactions" value="533"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G9K2"/>
    <dbReference type="GeneID" id="9185332"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003360001"/>
    <dbReference type="eggNOG" id="KOG2882">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043473_0_0_1"/>
    <dbReference type="InParanoid" id="D5G9K2"/>
    <dbReference type="OMA" id="PPMHRET"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004035">
        <property type="term" value="F:alkaline phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008967">
        <property type="term" value="F:phosphoglycolate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000039">
        <property type="entry name" value="Phosphoglycolate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006357">
        <property type="entry name" value="HAD-SF_hydro_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006349">
        <property type="entry name" value="PGP_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01460">
        <property type="entry name" value="HAD-SF-IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01452">
        <property type="entry name" value="PGP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288">
        <property type="entry name" value="4-NITROPHENYLPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288:SF46">
        <property type="entry name" value="HALOACID DEHALOGENASE-LIKE HYDROLASE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13344">
        <property type="entry name" value="Hydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13242">
        <property type="entry name" value="Hydrolase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000915">
        <property type="entry name" value="PGP-type_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="26"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="28"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00050247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00066659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00069197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR000915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000915-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000915-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000915-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ81195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="294" mass="31880" checksum="7349EE93758E407C" modified="2010-06-15" version="1">MPPQKLSGKPEELAAFIDKFDIFLFDCDGVLWQGASLLPRTAETIAHLRSLGKQLLFVTNNSTKSRTTYQAKLQGLGIPCGINEIFCSAYSSAIYISRVLRLQRDKRVYVIGESGIKEELATEGVAYISDDGGDITPEDYDSFGPDEDVGVVLCGLDHAISYRKLARAYQYLRNPGTVFLATNIDSTFPTHGKLFPGAGAMSAPLTYMTGRTPVSLGKPSQAMMDAIEGVLEFDRKRACMVGDRLDTDIRFGIEGGLGGTLAVLTGVSTEEEILEEGATVVPDAYLDRLCDILG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9Z8</accession>
    <name>D5G9Z8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00003471001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430068">
        <property type="protein sequence ID" value="CAZ81341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9Z8"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003471001"/>
    <dbReference type="HOGENOM" id="CLU_2795782_0_0_1"/>
    <dbReference type="InParanoid" id="D5G9Z8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ81341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="68" mass="7571" checksum="A6BD2F7CE8729302" modified="2010-06-15" version="1">MDFTRIRTYVIGTLPSAQSVASVSLKESVLLLASCCCCGLSVHIRVSYLRIPIEERIQNENAILDDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GA34</accession>
    <name>D5GA34_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00003550001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430071">
        <property type="protein sequence ID" value="CAZ81377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837186.1">
        <property type="nucleotide sequence ID" value="XM_002837140.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GA34"/>
    <dbReference type="GeneID" id="9187075"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003550001"/>
    <dbReference type="HOGENOM" id="CLU_2110719_0_0_1"/>
    <dbReference type="InParanoid" id="D5GA34"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="115" mass="13579" checksum="2807116BA3E02CC3" modified="2010-06-15" version="1">MAPSQFFSSVSFVFLQEINGWRGWVQTLIFFLIPWIQCSPCFFFLPPHFFSNFLHTCCPVVFHICAMPYFLVILLLLLYHHRLLSIVFRGKKKVKFLTRCRKSLKPISGSLYPFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAB2</accession>
    <name>D5GAB2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Autophagy-related protein 101</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00005219001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATG101 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430075">
        <property type="protein sequence ID" value="CAZ81466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837275.1">
        <property type="nucleotide sequence ID" value="XM_002837229.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAB2"/>
    <dbReference type="STRING" id="656061.D5GAB2"/>
    <dbReference type="GeneID" id="9185748"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005219001"/>
    <dbReference type="eggNOG" id="KOG4493">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069661_1_0_1"/>
    <dbReference type="InParanoid" id="D5GAB2"/>
    <dbReference type="OMA" id="VCWEIWT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990316">
        <property type="term" value="C:Atg1/ULK1 kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012445">
        <property type="entry name" value="ATG101"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13292">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13292:SF0">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07855">
        <property type="entry name" value="ATG101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0072">Autophagy</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ81466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="206" mass="23278" checksum="C1CEB7C8E9E16EC4" modified="2010-06-15" version="1">MSREGFPLQDDGMERPPVFSLDVLADRSLVKDIVKGVISTVFFHRFFPTVRPKTRDLLDLTLPAVDDPELDELINQRTNILIGSIEASAGVGTKPQRGEVVVYTVEFYERRPKKNWFSKAEEKVCWEQWVLSVTVVSPRTDAERIKLRMSMEAQLQAAVLNIITIASMKKDHIPPITTNEANPFPYSITVSPKGETWGARMGIFSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAT8</accession>
    <name>D5GAT8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="18">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">GSTUM_00005304001</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Regulates global gene expression after oxidative stress. Interacts and stabilizes mRNAs and may regulate their transition between different cytoplasmic components after oxidative stress.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Interacts with csx1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430086">
        <property type="protein sequence ID" value="CAZ81631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837440.1">
        <property type="nucleotide sequence ID" value="XM_002837394.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAT8"/>
    <dbReference type="STRING" id="656061.D5GAT8"/>
    <dbReference type="GeneID" id="9187798"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005304001"/>
    <dbReference type="eggNOG" id="KOG0108">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022096_2_0_1"/>
    <dbReference type="InParanoid" id="D5GAT8"/>
    <dbReference type="OMA" id="AWNANAF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02639">
        <property type="entry name" value="R3H_RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12253">
        <property type="entry name" value="RRM_PIN4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.50:FF:000002">
        <property type="entry name" value="Immunoglobulin mu DNA-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000183">
        <property type="entry name" value="R3H domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.50">
        <property type="entry name" value="R3H-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050502">
        <property type="entry name" value="Euk_RNA-bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034186">
        <property type="entry name" value="PIN4-like_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034069">
        <property type="entry name" value="R3H_Cip2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001374">
        <property type="entry name" value="R3H_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036867">
        <property type="entry name" value="R3H_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024642">
        <property type="entry name" value="SUZ-C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025">
        <property type="entry name" value="OS02G0815200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025:SF1">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01424">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00393">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82708">
        <property type="entry name" value="R3H domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51061">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51938">
        <property type="entry name" value="SUZ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="15">
        <location>
            <begin position="162"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="R3H" evidence="16">
        <location>
            <begin position="297"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="SUZ-C" evidence="17">
        <location>
            <begin position="494"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="250"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="473"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="250"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="270"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="475"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="495"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00055199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00062407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAZ81631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="564" mass="61793" checksum="F358CA303FD9CA26" modified="2010-06-15" version="1">MSTHHDYYDTIGSSRSPSGQHQPQQQHQTLHRVSSRHFETFASQSGLYTAEDHAARFDTNRYGSARLDTSMSGGYGYEASGAQTWNPSSFGGPGALAAMGGASGRIKTPSRQRSALPAMWTEPAPSLPTVSPLSAMGNVLLGPSPMRTETVGSDPEEELIPTAIVIKNIPFAIKKEQLVQLMTELGLPLPYAFNYHFDNGVFRGLAFANFTTAEETAAVIDTLNHYELQGRKLRVEYKKMLPLAERERIEREKRERRGQLEEQHRPVQMPLSQPNIPLSTSPSPSATRSKPDVDLNDPQTLQFYSQLLLFRDDVSREALIFPATLSPQQRRIVHTLAHHMALAHVSRGNGDQRQVHVFRAPISTTPSISPPSIPTTVHPVESQRRVLTRATTMDFSDARSEGAGIYSTMGRQAHALLDIPNSPSGRADALHSRQNLRAAKSFADLRSYTPSPVPSSASYPAALGGNISRYTDNYGSVTSTTPSLTPTATGTTVERQQRERERESNERENMSISTTGGGSNVVPTRQPRGPDSERAPGFASRRGQHHRASGGELDVQSGVEIVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBX3</accession>
    <name>D5GBX3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00005628001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ81973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837782.1">
        <property type="nucleotide sequence ID" value="XM_002837736.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBX3"/>
    <dbReference type="GeneID" id="9185408"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005628001"/>
    <dbReference type="HOGENOM" id="CLU_1004877_0_0_1"/>
    <dbReference type="InParanoid" id="D5GBX3"/>
    <dbReference type="OMA" id="SHINSTW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.462.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012951">
        <property type="entry name" value="BBE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08031">
        <property type="entry name" value="BBE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Berberine/berberine-like" evidence="1">
        <location>
            <begin position="219"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="281" mass="30857" checksum="8A30BBBE6DCDB690" modified="2010-06-15" version="1">MVSPASPGPHEAFWDAVAYIHGHLPRLADEGMTGYFVVMPLNGSLVFSWSFYLYERPPGTATALFSPIESHLKATYPSLGISTTETQYATWLEAYRPNIYPEPVGHNSLMASRLLPRTVLENNFENIRSTLVTVFPKVGLGGVVGQLVAAGQVSRNRHLHTAVHPAWRSAYLNLVIGVTWLDDTPLSAVLDIRKFNTEVSVGGLEKLALAGGGEDGVGAYVNEADAYTKKEDAGRVFWGAKYAELKMLKERWDPEGVFWCRLCVGNEGWVVEEGSGRLCQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GC00</accession>
    <name>D5GC00_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Rhomboid-type serine protease</fullName>
            <ecNumber evidence="10">3.4.21.105</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00005730001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Serine protease involved in intramembrane proteolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>Cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains.</text>
            <dbReference type="EC" id="3.4.21.105"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Membrane</location>
            <topology evidence="2 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the peptidase S54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.105" evidence="10"/>
    <dbReference type="EMBL" id="FN430098">
        <property type="protein sequence ID" value="CAZ82043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837852.1">
        <property type="nucleotide sequence ID" value="XM_002837806.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GC00"/>
    <dbReference type="STRING" id="656061.D5GC00"/>
    <dbReference type="GeneID" id="9181312"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005730001"/>
    <dbReference type="eggNOG" id="KOG2289">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022618_0_1_1"/>
    <dbReference type="InParanoid" id="D5GC00"/>
    <dbReference type="OMA" id="PIMIKPQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002610">
        <property type="entry name" value="Peptidase_S54_rhomboid-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22936:SF69">
        <property type="entry name" value="RHOMBOID-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22936">
        <property type="entry name" value="RHOMBOID-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 10" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="278"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="311"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="338"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="371"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="471"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S54 rhomboid" evidence="12">
        <location>
            <begin position="273"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="32"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="91"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="120"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ82043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="551" mass="60008" checksum="F0210FD12BE50073" modified="2010-06-15" version="1">MAAAEFYNTGPHPSGSHGPNDDDDYDRHNTHSPISSISNSASPVHKPLPSQPSFPPTHAATGYSDDFFGRPHSTQSIDTAYHPHEFDRPISPSHSSSYSSHDPYGKSAEPYNDDIPLNDTRPSQNSSHNLAAVEGGGFSGPKGKKGKSSFLKSNKGRRPWFCYIMAAIQISVFIAELARNAALTKTPIAIKPQFNPMIGPSVHVLINMGARFVPCMKTIKDITDKPGISYPCPNTTVEDTSREGCTLAKWCGFNGANIPSDLGGAAQAGSEPNQWYRFITPIFLHAGLIHIAFNMLVQLKLGTEMERDIGHLRFAIVYFAAGIFGFVFGGNFAPNGQPSTGCSGSLFGIFALMLLDLLWTWGSRKSPKKDLAFLLVEIIICFVIGLLPGLDNFSHIGGFLMGLFLGLTVLHSPPSIRQKIGAGEPPYTPMTTNRPPYAANPHSTLPGGFGGFLKNPAGFFKGRKPLWWAWWLVRAATLATALIVMVVLINNFYKYKKTCGWCKYLSCLPVLNWCEMGALTMTNTTTSSDTSDNPARMLARSLAPQDLAQFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>D5GC67</accession>
    <name>D5GC67_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00000605001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430103">
        <property type="protein sequence ID" value="CAZ82110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837919.1">
        <property type="nucleotide sequence ID" value="XM_002837873.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GC67"/>
    <dbReference type="STRING" id="656061.D5GC67"/>
    <dbReference type="GeneID" id="9184786"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000605001"/>
    <dbReference type="HOGENOM" id="CLU_1950379_0_0_1"/>
    <dbReference type="InParanoid" id="D5GC67"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037393">
        <property type="entry name" value="Bud22/SRFB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015158">
        <property type="entry name" value="Bud22_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23325">
        <property type="entry name" value="SERUM RESPONSE FACTOR-BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23325:SF1">
        <property type="entry name" value="SERUM RESPONSE FACTOR-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09073">
        <property type="entry name" value="BUD22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bud22" evidence="3">
        <location>
            <begin position="3"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="22"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="68"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ82110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="129" mass="14243" checksum="E1E95CFD3046C030" modified="2010-06-15" version="1">MPKQKPGVSTFLPTFIAGYISDSSASTSSTAPPKRNKGPPEKKQRKNRMGQQARRALWAKKYGQNAAHLKEGTQRKEDRRGGGEKRKKEGQTEGKLHPSWEAARKAREGKMAVVQEALRGGGGRKVVFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCA6</accession>
    <name>D5GCA6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00000652001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430108">
        <property type="protein sequence ID" value="CAZ82149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837958.1">
        <property type="nucleotide sequence ID" value="XM_002837912.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCA6"/>
    <dbReference type="STRING" id="656061.D5GCA6"/>
    <dbReference type="GeneID" id="9184827"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000652001"/>
    <dbReference type="HOGENOM" id="CLU_2028414_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCA6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.20">
        <property type="entry name" value="Ubiquinol-cytochrome C reductase hinge domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036811">
        <property type="entry name" value="Ubol_cytC_Rdtase_hinge_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="122" mass="13678" checksum="9182BEB670986563" modified="2010-06-15" version="1">MPLSSLFSDIYTSLTISPVNAEAPPVEEHEGSSDLKAEEKRSAEPEPKEEESAGGGAETEEEEPEDVMGAITEGIMLRVKRMQASKASLRRRRSRRRIVWRIFHLMHCSAQCAAPKIWAKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCJ3</accession>
    <name>D5GCJ3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00005917001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CENP-L/IML3 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430109">
        <property type="protein sequence ID" value="CAZ82236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838045.1">
        <property type="nucleotide sequence ID" value="XM_002837999.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCJ3"/>
    <dbReference type="STRING" id="656061.D5GCJ3"/>
    <dbReference type="GeneID" id="9186418"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005917001"/>
    <dbReference type="eggNOG" id="ENOG502S6KJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034400_1_0_1"/>
    <dbReference type="InParanoid" id="D5GCJ3"/>
    <dbReference type="OMA" id="SRAAWMF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025204">
        <property type="entry name" value="CENP-L"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31740">
        <property type="entry name" value="CENTROMERE PROTEIN L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31740:SF2">
        <property type="entry name" value="CENTROMERE PROTEIN L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13092">
        <property type="entry name" value="CENP-L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0137">Centromere</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ82236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="299" mass="32818" checksum="DAEE5F95662CFF8D" modified="2010-06-15" version="1">MSRNPPQSSKSPVHPLYNVTYTIHRLSPLHKFPPRSDFKSHARSLQQILRGDVLRGVRIALGNEDEALGRAGQLKAVTWEALPRWQTFSEDDEEEAAVEEAGPGVIIEMRYEKMSYTSLLLPSPSEATGLEAESQEFTKLPLLLTRLPKSLRDTLISYLSTRFDTLPLPLALPSSLLQGSVEFYFSHTYSPPKDVQISFATPTTTLKTVTITIPRDDIAEFCARGKQLPGGGFYAALKHYLRGAMALDITSLAIAKVACGGFVLGGGTGGKIKIFPPRDVGEHEAVKNLIQELVKVADT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCN5</accession>
    <name>D5GCN5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00000746001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430118">
        <property type="protein sequence ID" value="CAZ82278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838087.1">
        <property type="nucleotide sequence ID" value="XM_002838041.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCN5"/>
    <dbReference type="FunCoup" id="D5GCN5">
        <property type="interactions" value="70"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GCN5"/>
    <dbReference type="GeneID" id="9187896"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000746001"/>
    <dbReference type="eggNOG" id="KOG0740">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_15_1_1"/>
    <dbReference type="InParanoid" id="D5GCN5"/>
    <dbReference type="OMA" id="PIMLEDF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000022">
        <property type="entry name" value="Fidgetin like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036181">
        <property type="entry name" value="MIT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050304">
        <property type="entry name" value="MT-severing_AAA_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015415">
        <property type="entry name" value="Spast_Vps4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074:SF17">
        <property type="entry name" value="FIDGETIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09336">
        <property type="entry name" value="Vps4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116846">
        <property type="entry name" value="MIT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase AAA-type core" evidence="5">
        <location>
            <begin position="547"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="domain" description="AAA ATPase AAA+ lid" evidence="7">
        <location>
            <begin position="676"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="domain" description="Spastin/Vps4 C-terminal" evidence="6">
        <location>
            <begin position="721"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="135"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="265"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="351"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="169"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="299"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="403"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF09336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ82278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="758" mass="82609" checksum="C48EFBD8C2D9ED96" modified="2010-06-15" version="1">MKAKAYSALQKTYDQAFLIRSTAVYYEDQGNEVEALRCWRECLGQLQGHNSAGHRPRSETERTLLRSLKAMQDQCQERSELLQTLLISRVEAENNGQGWRNKDADPATSSLQAAITAGNVKPAVLPIPYPLPPPVPPRRLPSSGSSSVRDTSRVIPPSLHPASAPPPNITHSSSSRTPSPEKRAFLSTLRPTRKQKKTDSRIINDGPEAAAKAATLAWSSLSRAEGAHLSRAMAAPSTDPLPVRQSFVASASTTNDISCGQRASTFTVHRRPSPPPAMLSRTGLSGIRSRPNSSILPLGNPPCPGDSMPPAPPHGHVPPSTDNMRTSHLLHRELLQALQTPAETAHCYSCAGSQSAPTFAPRVPPKPAKSKRLSRVNNSKPVIPTPELESKEDSDSEYYKPPTATNSRNTIHQPAAPSGGDRSTGPPTPSPEEEKLITSTGGEEALPPPENTLDGRVAKALESIGRGVDMNAAMQVANEIVVKGDEVHWDDSTHFCDQIYSAVCESRHGGCSYLGLRELGKPCLPELSQRSPSLRFSPFLPLHLHLNMQLGESEKLVRALFALAKAMAPSIIFVDEIDSLLSSRSGPGEHEATRRIKTEFLIQWSDLTKAAAGRESAEGDASRVLVLAATNLPWAIDEAARRRFVRRQYIPLPEDDTRKKQLKNLLGRQKHHLSAEDIERLIELTQGFSGSDITALAKDAAMGPLRSLGEALLQMQMDDIRPITFEDFEASLMSIRPSVSKEGLRAFEDWATKFGERV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCU3</accession>
    <name>D5GCU3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00000750001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430123">
        <property type="protein sequence ID" value="CAZ82336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838145.1">
        <property type="nucleotide sequence ID" value="XM_002838099.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCU3"/>
    <dbReference type="STRING" id="656061.D5GCU3"/>
    <dbReference type="GeneID" id="9182441"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000750001"/>
    <dbReference type="eggNOG" id="ENOG502S2MG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028361_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCU3"/>
    <dbReference type="OMA" id="WHGNHVW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006614">
        <property type="entry name" value="Peroxin/Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051513">
        <property type="entry name" value="Tectonin_beta-prop"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23250">
        <property type="entry name" value="DYSFERLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23250:SF1">
        <property type="entry name" value="TECTONIN BETA-PROPELLER REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00694">
        <property type="entry name" value="DysFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peroxin/Ferlin" evidence="2">
        <location>
            <begin position="242"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="308"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="420"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="515"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="46"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="420"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="454"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="534"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ82336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="545" mass="60537" checksum="14FCCDDE0608B4CC" modified="2010-06-15" version="1">MASLLSFRASRSFNSSSGELNLRINLQDNTVSQPPTAPASEAGDVPAQPSNASATSSAAPGRRETLRKAIAQRKYRGWTIDPATSSVGDELEIAGGDGAPDPRAGGVVERTGEEDIEIVVPSGGGGEDGSLQERGRERSNTGGNARRDSAEHVPERRQSQRWRKKEIAEVDILYENQRGVFACGIPLFSSKGLLNLDPAPWQNGLFRDSAVSIVNAQLPDPSWAWAWKTWYVDMTQDVDEEGWTYSFSFNPAFSWHGNHVWFHSFVRRRRWIRKRIKMSYDPDFSSREGSQERPHGLNQDYFTIHSRRLADGGSTGTGAGKRKQRRSVMEGSEWTGQNAEMDDDLEEEGLITDIPKLLRVLRIARLDREKIEVVGKFLKDGGEEVTYLPERIPHIMSLMIFQASRRQLLKLLIAAADEFTPSQPSTPSQPSTPSQPSSTVLTPHAEPSDLLPTDGSSTPQSGSAVELQSGTQKADRKAARQRKHESLKKAIETAEQEVRRLEYWSDVKEVAAETEGGMRIAEEQTGLPLGMDDSELKWKGKEVER</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCV7</accession>
    <name>D5GCV7_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dynein heavy chain, cytoplasmic</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Dynein heavy chain, cytosolic</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">GSTUM_00000794001</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Mel28</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Consists of at least two heavy chains and a number of intermediate and light chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein heavy chain family.</text>
    </comment>
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        <property type="protein sequence ID" value="CAZ82350.1"/>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
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    </evidence>
    <sequence length="4187" mass="473147" checksum="8DA76EB586A46474" modified="2010-06-15" version="1">[encoded data, 4187 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>D5GD19</accession>
    <name>D5GD19_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00000937001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="project" value="GOC"/>
    </dbReference>
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        <property type="term" value="C:RZZ complex"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046362">
        <property type="entry name" value="Zw10/DSL1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055148">
        <property type="entry name" value="ZW10_C_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12205">
        <property type="entry name" value="CENTROMERE/KINETOCHORE PROTEIN ZW10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12205:SF0">
        <property type="entry name" value="CENTROMERE_KINETOCHORE PROTEIN ZW10 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22766">
        <property type="entry name" value="ZW10_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ZW10 C-terminal helical" evidence="2">
        <location>
            <begin position="698"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="476"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ82412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="848" mass="93687" checksum="8455D1D723BBB893" modified="2010-06-15" version="1">MASSKIKPSQLADAVLSSVLDGSYPDSDDVAGAVLEASALPEIIAPLQKARESLYDGVRTVSRESGPEVDSWISQARGLHKDIESSKTKADEIVQLAEQEKRLNLELEDSIMQARLLVSEINFNEALAEILSKLQLIDLTLYQVKHLLNTGELCQAIEALDNVEVALEKLPVSGETIVISLMKEKAKGLRSMLVNKLEDCWRYLLRINPGKGGISIDRDAAGSCPLVESVIEALKKLDLLNGKIDHLHHQIDKLLVAPRFDSIKGSAPTFSVAGNSICMGGNTNDLSATRMFNDLRQLITFFNRNLPSGITPLFSRIFIANLTTRLMSSLSSSVPSSLDDLPKFEALLKETREFEKFLHQISWTKETELREWTERAPKVWLSKRKETSLDKARQILSRGTSDVKVVERTETQEMKVASPTERRKSVGAPMDVWNDDCGWGYEDAPVDTAAAADSLQIDMDDDDASGWGLDEDIDIEDEEQKDEKSSQPVDVPTEEDGLDNLDWGEWGEDDPGIEARESMAASDTAPAKKPTESKPAIRYSSSHSGDITLRESYTITSIPDAIMEIIVRVADEAKRLSNMPPSAVTPAASGMLSIPSLVLAAYRALAPTSYPTNISSNMYVYNDCVRIAEQLQDLEVTVELTHPNNNWDTDINIIQSFGKRHYSKEIGLQRTVLCDFLDGAKGFVGCTNFPQSADCQTAISSVVARINGIYTSWSQVLSPSVLFQSVGLLLNTVVTKLINDIEDIPDIPAADSEKICTFFEEIATLESLFPDAPGGAGVSTTGFHCKNWLKFRFLQQILDSRIADILYMFREGHLEGFETEELVDLVKALFADNDNRKKCIDEIRSGGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5GD29</accession>
    <name>D5GD29_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00000917001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Luc7 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430137">
        <property type="protein sequence ID" value="CAZ82422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838231.1">
        <property type="nucleotide sequence ID" value="XM_002838185.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GD29"/>
    <dbReference type="FunCoup" id="D5GD29">
        <property type="interactions" value="877"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GD29"/>
    <dbReference type="GeneID" id="9183885"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000917001"/>
    <dbReference type="eggNOG" id="KOG0796">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030397_1_0_1"/>
    <dbReference type="InParanoid" id="D5GD29"/>
    <dbReference type="OMA" id="CPYDLFQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006376">
        <property type="term" value="P:mRNA splice site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004882">
        <property type="entry name" value="Luc7-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12375">
        <property type="entry name" value="RNA-BINDING PROTEIN LUC7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03194">
        <property type="entry name" value="LUC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="218"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="228"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="249"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ82422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="300" mass="32641" checksum="62DDD59EFA203FA6" modified="2010-06-15" version="1">MGTPNQTNSSVPFTDPSVCRSFLVGMCPHDLFTNTKQDLGPCKKTHLESHKADYERERANKPSKDLGYEYDYQRDLQRYIDECNRRIDAAQRRLDKTPDEITKTNSLLKGIADLDGSISSGLQEVEVLGETGAIGRALDEWHKVKVAKMEKEGKEKELKLLSDSAGPSGHQKLQVCDVCGAYLSRLDNDRRLADHFYGKMHLGYAQMRSTLKKLQEELAGRGPPQGQHGRDFRDNDRGFHDGGYSREQSGGGGYRGGFRGRGGGYRGGRSGDGGGGWGGRGGGGGGYGGRGGGGGGGHRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GD49</accession>
    <name>D5GD49_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00000974001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430140">
        <property type="protein sequence ID" value="CAZ82442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838251.1">
        <property type="nucleotide sequence ID" value="XM_002838205.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GD49"/>
    <dbReference type="GeneID" id="9183906"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000974001"/>
    <dbReference type="HOGENOM" id="CLU_2147699_0_0_1"/>
    <dbReference type="InParanoid" id="D5GD49"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="112" mass="12280" checksum="61279A2E978A523F" modified="2010-06-15" version="1">MGKEKKAELDINKIDSATLPNLSTTVHRKLHCDVMNAVSGHFEPIARIPASQPTRAFHPEPMSVTPAFSFKARASHRQSHPSRKASQSPINSHVRSLRQAVAMIPKILAVAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDC3</accession>
    <name>D5GDC3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00006146001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430142">
        <property type="protein sequence ID" value="CAZ82516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838325.1">
        <property type="nucleotide sequence ID" value="XM_002838279.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDC3"/>
    <dbReference type="STRING" id="656061.D5GDC3"/>
    <dbReference type="GeneID" id="9183980"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006146001"/>
    <dbReference type="HOGENOM" id="CLU_1826696_0_0_1"/>
    <dbReference type="InParanoid" id="D5GDC3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="75"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="141" mass="15268" checksum="A7BE31023A5613D6" modified="2010-06-15" version="1">MRNSVEADAGEATKEVKAETVVRGDDAVPPAIKEGTTTTKPEEAITLSTTEPERTTTLVAKAEKLLGKKKRKGVVEGLSSPPLESLSLPAQLQFVPSAPPSRSISPGPRLKRSKRDDPTKPGRKKKKRKKRDEIDDLFAGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDE0</accession>
    <name>D5GDE0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">GPI ethanolamine phosphate transferase 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">GSTUM_00006167001</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 13">Ethanolamine phosphate transferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers ethanolamine phosphate to the GPI second mannose.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Endoplasmic reticulum membrane</location>
            <topology evidence="1 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the PIGG/PIGN/PIGO family. PIGG subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430142">
        <property type="protein sequence ID" value="CAZ82533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838342.1">
        <property type="nucleotide sequence ID" value="XM_002838296.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDE0"/>
    <dbReference type="FunCoup" id="D5GDE0">
        <property type="interactions" value="447"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GDE0"/>
    <dbReference type="GeneID" id="9183996"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006167001"/>
    <dbReference type="eggNOG" id="KOG2125">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004770_0_0_1"/>
    <dbReference type="InParanoid" id="D5GDE0"/>
    <dbReference type="OMA" id="SWNQTGQ"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051267">
        <property type="term" value="F:CP2 mannose-ethanolamine phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16024">
        <property type="entry name" value="GPI_EPT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000045">
        <property type="entry name" value="GPI ethanolamine phosphate transferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037674">
        <property type="entry name" value="PIG-G_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039527">
        <property type="entry name" value="PIGG/GPI7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045687">
        <property type="entry name" value="PIGG/GPI7_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23072:SF0">
        <property type="entry name" value="GPI ETHANOLAMINE PHOSPHATE TRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23072">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL GLYCAN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19316">
        <property type="entry name" value="PIGO_PIGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 13" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="434"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="467"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="492"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="580"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="623"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="656"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="679"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="708"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="742"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="787"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="822"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="domain" description="GPI ethanolamine phosphate transferase 2 C-terminal" evidence="14">
        <location>
            <begin position="430"/>
            <end position="847"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00056729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU367106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF19316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAZ82533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="852" mass="93777" checksum="EAF6E9DDC25B7C2D" modified="2010-06-15" version="1">MKASMSWLLLVVTNLLLPAGLLLFAKGFFPYKPFLPGLAEFDGGVSSAPKMFDRVVFMVVDALRSDFVFSEESGFGFTQSLISSGSAIPFTAHATSPTITMPRVKGLTTGSVPGFLDVILNFAESESAVTLASQDNWLAQIVNRGGKLVMYGDDTWAKLFPGMFTRSDPTSSFFVSDFTEVDNNVTRHIDNELQRDDWEGMIMHYLGVDHIGHKAGPESPNMIPKQQEMDGIVRRIFTAIEEREHLKNALFVLAGDHGMNDAGNHGGSGLGETSPALVFMSPKFKKSFSGMKCPAEFREGFDYYTTVEQNDFVPSLAGLLGFPVPRNNLGVLIPSLLHMWNVEDRISLALANAKQLLTIVKAAHTDFDSRNVGPIDCIDVRDDVDGLACLWNESVSSRYFTEGKENPEVALVSLLKFSAKCQDVLSSASSDYDLTLMATGIAFVLLGLLCSLIGLVPILERSRLANVMFFATTATYVVMMFASSYVEEEHNFWYWVASGWLFLLFLKVNSDAFRIFLIIPMLRVARRWNQTGQKHAGASDITKDFLSEKVNLLWFLVLFTYTDMFGRIVKNTFRNIPGRAAGFIFGFSTIGAALTFKLCFAAQDTPDLIADWLKPLVLYTEELDLSLISLARTTFISLGTIILYTILFETRKKGPNVTHTISTILLTLLPIFLATQTKLLNIPLLYLLTLTIRLLSSSSSLTSTDLTLSTLILSHFAFFAYGGANAISSVDLSNSYNGVSGYSIAIVAFLTFVSNWIGPIYCSSAATVAITIAARKREDGAGLTRHLMCLSLFCCAATGAVMLACTVLRTHLFIWTVFSPKYLFAMVWVVWHLGANLLLGGVLVLVGRVGLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDN0</accession>
    <name>D5GDN0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00001127001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430152">
        <property type="protein sequence ID" value="CAZ82623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838432.1">
        <property type="nucleotide sequence ID" value="XM_002838386.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDN0"/>
    <dbReference type="STRING" id="656061.D5GDN0"/>
    <dbReference type="GeneID" id="9181627"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001127001"/>
    <dbReference type="eggNOG" id="KOG2169">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_326574_0_0_1"/>
    <dbReference type="InParanoid" id="D5GDN0"/>
    <dbReference type="OMA" id="NIPAGEW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061665">
        <property type="term" value="F:SUMO ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16650">
        <property type="entry name" value="SP-RING_PIAS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004181">
        <property type="entry name" value="Znf_MIZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782:SF4">
        <property type="entry name" value="TONALLI, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782">
        <property type="entry name" value="ZINC FINGER MIZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02891">
        <property type="entry name" value="zf-MIZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51044">
        <property type="entry name" value="ZF_SP_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SP-RING-type" evidence="6">
        <location>
            <begin position="748"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="244"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="374"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="491"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="260"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="419"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="433"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ82623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="882" mass="97686" checksum="8B784905B7129DA9" modified="2010-06-15" version="1">MVSPSQVAGSSGFAPLNSPIQTAYFPTTSSQADLSQYRAALSAQTQLQTTNQVQPAQPVQLQQTVGGGPRLELLLPNYDSYIQREGGPGCQKFNSAECFRFRIMREAIVANDWFFAVLHQLYTLALVNERMLASYTKVTANMPGVALMSEILHDGVKPRQKYLTYLSTMPVPLPDALKQLSYYHKYVGHVSRFMADGYKVVPIMRDYYVKRGVAPNVDVIEHGWKVTSPTMCNVIARYIQSHVSTGQNVPSPSPNPQPQPQASQRRQQQQQQQQQQQQQQQQQQQMRLQQMQHARQNGQNNPNLVMARQLPGGDSYQLLAEQQNQQIALNHMALSRAAGNPQGHRPSLHINSATDGFTRDPAIALQNAPDAGLLQAGSPVSATSPFTGPGRQPLAGSPMGQHSPNALTAPAQDVERRSSVASQGRASRTVGTRQGQPQPQHLQQIQHLQQMQHMQHLQQQQQQQQQQRGMLQIAAQVARDQRQLQNAHSNIPAGEWNGLFTGPPPNSASLGMPSPSVFPPTFPLPSSQRAHGNLPQNVLQSHLATPPFSPPPPSSVAPPGRLPLYISLTQFALKDIILDSQRISQTFHWVMGAEAESRLSKKRASTKPGEPTTRPMVQPGSLIYRLRSVKVTDSSRTPRTGGEWVVQETAWPAHIFIEVNKQVIEVRRKVAWGKDLPADITSYVQAGKNELKVVCLFPGKQRDPSTFVMAIEIYECLSEDSIIKDIKHIPLSSAKSSILGRLSGASDTDDVVLVESDRVSLGVRCPLSFTLLTTPVRGVTCKHLECFDLQNYLETRPRRKDHEPPFADSWRCPLCRGDARPTELVVDDFLASVLQELMLSGSTDVQNIMVKRNGSWEPVRKEEEKKDGGKVNGTVEIICLDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>D5GE13</accession>
    <name>D5GE13_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00001173001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430161">
        <property type="protein sequence ID" value="CAZ82756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838565.1">
        <property type="nucleotide sequence ID" value="XM_002838519.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GE13"/>
    <dbReference type="GeneID" id="9183096"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001173001"/>
    <dbReference type="eggNOG" id="ENOG502S233">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008809_1_2_1"/>
    <dbReference type="InParanoid" id="D5GE13"/>
    <dbReference type="OMA" id="CPELWNS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35041:SF6">
        <property type="entry name" value="FORMYLMETHIONINE DEFORMYLASE-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35041">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="133"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="170"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="236"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="601"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ82756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="688" mass="75056" checksum="D2E42A041F10C4CD" modified="2010-06-15" version="1">MSSHQRRTGDPAPPSPTGDANSDFGLRNGSGHNRAWGATLDPTHNPLLRQPSSSSPSFEDGDESVRSELHYYLTNPPQRNPTRRISIPRKPLSACPPATAIHTSSDPGRTSGSPAEPIPPKHWMRALGALSPILMMVELFFLGVAFTIGHYVFYKSWRDSIVEENLGQEYIIWAGTAFAFLSKASLVGAVVVAHKQIAWDTVRKKAITVDGVDAMFVAPTDFTSFWNRDMLKRAKVATILALLAWCVPISAVITPGSFSVSLVQRQFNTSKPIPSTNFSDPTGFVTSLTLTVLQDGTGKTNAPEAISGATIAPSALLSRIVSTTATSGTILPIKPPQPNSSYVNSFHGPSLRCTPADLRATQAIRRTMNYDESGRIRYATIYNPEISGDTCSIYVGLAYNTTTVQNRTYSICGPNGGGCMVNFAQETKFDDPSMGQAFSCELYNTFFTVNVTFRDSTQTLGIQKLEYLNPVSCAPLAPPVTNRQPFAEWKASYAVFAAMGDILVGNITKNRDSGFRGLNPVSWDTRILQTTLIGSSDFANMNITNRPNDMYTGPLARGIEELSRNITLSLLSSDTFGLPIKSDVLVTRQDTRYLFNERSFWLAYGIAIFVAAGAVLAGIVAYYANGVSLEPNFSTVMATTQSDEMQELVRGVNVDFRELQKVVGGREVRLRKLQNGDRRFVVVGEVKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEG2</accession>
    <name>D5GEG2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00006472001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430175">
        <property type="protein sequence ID" value="CAZ82905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838714.1">
        <property type="nucleotide sequence ID" value="XM_002838668.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEG2"/>
    <dbReference type="FunCoup" id="D5GEG2">
        <property type="interactions" value="322"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GEG2"/>
    <dbReference type="GeneID" id="9181536"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006472001"/>
    <dbReference type="eggNOG" id="KOG3100">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075129_1_0_1"/>
    <dbReference type="InParanoid" id="D5GEG2"/>
    <dbReference type="OMA" id="MELPTHQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039883">
        <property type="entry name" value="Fcf2/DNTTIP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014810">
        <property type="entry name" value="Fcf2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21686">
        <property type="entry name" value="DEOXYNUCLEOTIDYLTRANSFERASE TERMINAL-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21686:SF12">
        <property type="entry name" value="DEOXYNUCLEOTIDYLTRANSFERASE TERMINAL-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08698">
        <property type="entry name" value="Fcf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fcf2 pre-rRNA processing C-terminal" evidence="4">
        <location>
            <begin position="155"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="63"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="246"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="72"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="265"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ82905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="275" mass="30746" checksum="8A61EA43944A6585" modified="2010-06-15" version="1">MPTEETPGLHKESAEAEAVPEGNRIDVVEGPPARLFSSSHVRSDGFGDDVAELSDETVGRLLSEAEERMKSSSKTLSTSSSTNRSKTLMTKFPKLNPGASLPKAPIKSQGAVSRISDPSLLVPSSDRKLANTSAAAGGVSLVDPALSKKRLKEDEKTAGLKWFDMPRTNLTPEFKRDLQLIKMRGVLDPHRHYKKDNSSFPEYSQVGTIVEGNADYFSSRLRNKERKRTIAEEVMADDSSRKRFKNKYEEIQRKKRSGKKEFYKKLKQQRKKSSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEQ1</accession>
    <name>D5GEQ1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00001325001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430183">
        <property type="protein sequence ID" value="CAZ82994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838803.1">
        <property type="nucleotide sequence ID" value="XM_002838757.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEQ1"/>
    <dbReference type="STRING" id="656061.D5GEQ1"/>
    <dbReference type="GeneID" id="9184905"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001325001"/>
    <dbReference type="eggNOG" id="ENOG502SNQ1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_746052_0_0_1"/>
    <dbReference type="InParanoid" id="D5GEQ1"/>
    <dbReference type="OMA" id="CEMERLA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053178">
        <property type="entry name" value="Osmoadaptation_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38111">
        <property type="entry name" value="ZN(2)-C6 FUNGAL-TYPE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ82994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="360" mass="41007" checksum="62B39D9871E03A2F" modified="2010-06-15" version="1">MAVCTVFFGRQSRDERIVKESTKIYTRALAQLNAALSNKTMHTRDETLAATMLLGMYETMGCADPLTWQRHADGMELLVRVRGPEAHTTELGHRLFTSFRGTSLTNSLVSCKRNFLASKEWLTIPWTKTQKTPMQQLQDLHLELPGILEARRALPRSAHPSLLRRCLDFKAKLEAWYENFISTPSAHHWLEFSALNIDPPVFPTCYRFPNIGVANTYTFYWANLIVLNYTIIGLTPACQSPLVTTDEIYDLAAQICMSVEYFVAPERKSYGPVLTMYPLRVATGCFMRMGARGKKSTLWCKAIFGVLGERGVMLAKMLEMVVWENKETEEAESVGTVHEWAKKEFWGRVEEGLDEPETEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>D5GET8</accession>
    <name>D5GET8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00001381001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430193">
        <property type="protein sequence ID" value="CAZ83031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838840.1">
        <property type="nucleotide sequence ID" value="XM_002838794.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GET8"/>
    <dbReference type="STRING" id="656061.D5GET8"/>
    <dbReference type="GeneID" id="9184943"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001381001"/>
    <dbReference type="eggNOG" id="ENOG502R71Z">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1760128_0_0_1"/>
    <dbReference type="InParanoid" id="D5GET8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ83031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="148" mass="16064" checksum="FEB2DCB26FFE3D4E" modified="2010-06-15" version="1">MESQSPPSPESPQSLLHSPSFLSILRVGISHSHTPQCLWPKDKGRVVVEPALHWLFATLYKGAAFGYPQAAAEETGYGSELEAAIKGLKGMHRRGITILPRGDYGCAWTPHGTYACDLAHFAKHLGDYADCIQVNGNALEDISILQGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFD4</accession>
    <name>D5GFD4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00006840001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430208">
        <property type="protein sequence ID" value="CAZ83227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839036.1">
        <property type="nucleotide sequence ID" value="XM_002838990.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFD4"/>
    <dbReference type="STRING" id="656061.D5GFD4"/>
    <dbReference type="GeneID" id="9188083"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006840001"/>
    <dbReference type="eggNOG" id="ENOG502S3FT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026731_2_0_1"/>
    <dbReference type="InParanoid" id="D5GFD4"/>
    <dbReference type="OMA" id="WRLPYGD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040144">
        <property type="entry name" value="RAP1GDS1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10957">
        <property type="entry name" value="RAP1 GTPASE-GDP DISSOCIATION STIMULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ83227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="603" mass="65978" checksum="EA1B4137FB20A266" modified="2010-06-15" version="1">MSTVDTINTLLEDTRNDVAVIVSILERLKAEGGNGFVKGAEKCGDACRQAEWRQILGDSGILGFFLDQLDKEVEFLLGRQIFRVIANSIADNDGNRQRLIDHPTALKTVLSLVSSEDLSMFATATLQNLTLDNEVAQLKVIEAGGVEVLMELVGTRGDGSVDIQVSLRVLELLVPHDTAKQVTPPTCIRPLLSKLSTEVTPIDDRILLLTILTTLLTHSPLQLAILEQNQFPLFLQVFEMTHSLLFDPALDITDRKRLFSAKSPLMDNIADISWKPTFLATYPLSSAPIAKILTWLSASNEPHRYDKVIAACLVFGNVTQSTEVCRELIHTYALHKTLFSVIHRAVEGYEQSKKDLESLPAEVKLDSAGAGKGGIYLSLLHSAIGVLRNFSVPKSEKAILVDAGALDAILAAVGMEGVGVGQVWYSAVGLARLLCVDCVENCKKITSPLEPGEITCLEKLLQTYTQAEELPTKTEIARMIVSILRCLSSLPEDPPTAYHLGLARPLWDMVLQDKWVVVRSEGLLGLALLAKGSEAGELWQEWGDKEWEVVDKLDGRDLENVGVMIVNLRNGECADVIAGNKRVEGLLGKCLAGKMAEVQVEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>D5GG31</accession>
    <name>D5GG31_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mitotic-spindle organizing protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Mitotic-spindle organizing protein associated with a ring of gamma-tubulin 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00001971001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for gamma-tubulin complex recruitment to the microtubule organizing center (MTOC).</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the gamma-tubulin complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Spindle pole body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MOZART1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430236">
        <property type="protein sequence ID" value="CAZ83474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839283.1">
        <property type="nucleotide sequence ID" value="XM_002839237.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GG31"/>
    <dbReference type="STRING" id="656061.D5GG31"/>
    <dbReference type="GeneID" id="9184538"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001971001"/>
    <dbReference type="eggNOG" id="ENOG502S6UI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1723656_0_0_1"/>
    <dbReference type="InParanoid" id="D5GG31"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000931">
        <property type="term" value="C:gamma-tubulin ring complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031021">
        <property type="term" value="C:interphase microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033566">
        <property type="term" value="P:gamma-tubulin complex localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051415">
        <property type="term" value="P:microtubule nucleation by interphase microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090307">
        <property type="term" value="P:mitotic spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022214">
        <property type="entry name" value="MZT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28520">
        <property type="entry name" value="MITOTIC-SPINDLE ORGANIZING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28520:SF2">
        <property type="entry name" value="MITOTIC-SPINDLE ORGANIZING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12554">
        <property type="entry name" value="MOZART1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ83474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="152" mass="16705" checksum="8236E51199ED6575" modified="2010-06-15" version="1">MPDLLHAPPTGGKPIAPPSPHVPLTLVLYTAMTSPLPADWSYNTILPKTIPLNSQRFQPYTLVTASRLFRPPHTTSAKNTMSTRPSKQQAAREVIDILHEISTLLNTHLDRTTLSLCVSLVENGVHPDALAAVIKELRRESGQLNMGQLEET</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>D5GG86</accession>
    <name>D5GG86_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00002000001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the asteroid family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430246">
        <property type="protein sequence ID" value="CAZ83529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839338.1">
        <property type="nucleotide sequence ID" value="XM_002839292.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GG86"/>
    <dbReference type="STRING" id="656061.D5GG86"/>
    <dbReference type="GeneID" id="9184590"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002000001"/>
    <dbReference type="eggNOG" id="ENOG502S2DC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016461_1_0_1"/>
    <dbReference type="InParanoid" id="D5GG86"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026832">
        <property type="entry name" value="Asteroid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039436">
        <property type="entry name" value="Asteroid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15665">
        <property type="entry name" value="ASTEROID PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15665:SF1">
        <property type="entry name" value="PROTEIN ASTEROID HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12813">
        <property type="entry name" value="XPG_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Asteroid" evidence="3">
        <location>
            <begin position="152"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="526"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="526"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ83529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="593" mass="65958" checksum="BA2E67F938AFCE84" modified="2010-06-15" version="1">MGIRRLYTNLRPYARPTILEPCTEIFIDGPGLAHHVYEKCYSAAPPARPEDVNPWDSAIPYSVLQDSVIKYLHMLESCGAKMIYFDGALPASKRPTRILRLEESLRKLCTFHASYSHTLPRCSDRSAREWGGVEIFSERAVYTPQSPVLSTPPFLVTATLAALSRGGYAERTSTVPGEAEGYAARDAIAAGGVVLTSDSDLLLFRGEGENDWGVVMFKDLEVNFSTGQISTVLFRPEDIAKGFDLELAFLGYLSLCNPQATFSTLREWCARITERSRSGAIVEEMGRKWREFAAGYALPEGDGGRLIDPRIGELLSLRGAVGRKREMFLPTLWEDTSRASAWDVGREVRALAYSLLFPGEMSLEEISRRGHGISGAPVEIILGRKAKQKLHEIILESQEEDWQARYVLQQIVKTFAARNKPPPHASILESSASLVSACAHTSTDATNRQTAGVRWTWERIQTFAMLQAAWYSLLLLSEVLKETTSSKSGLGVLPVEVLDGRRFVDARPGAGAKSLVKEILAAYFGEEEEEEEEEEEDMKARRRMSKEENLEGVWKEASGKARRKRRWPAEEVAEVKVKVAATKGGNMFAALVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGE7</accession>
    <name>D5GGE7_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Neutral ceramidase</fullName>
            <ecNumber evidence="5">3.5.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00007343001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>an N-acylsphing-4-enine + H2O = sphing-4-enine + a fatty acid</text>
            <dbReference type="Rhea" id="RHEA:20856"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:52639"/>
            <dbReference type="ChEBI" id="CHEBI:57756"/>
            <dbReference type="EC" id="3.5.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="4">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the neutral ceramidase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.23" evidence="5"/>
    <dbReference type="EMBL" id="FN430264">
        <property type="protein sequence ID" value="CAZ83590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839399.1">
        <property type="nucleotide sequence ID" value="XM_002839353.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGE7"/>
    <dbReference type="STRING" id="656061.D5GGE7"/>
    <dbReference type="GeneID" id="9183188"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007343001"/>
    <dbReference type="eggNOG" id="KOG2232">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011300_4_0_1"/>
    <dbReference type="InParanoid" id="D5GGE7"/>
    <dbReference type="OMA" id="RNTMKTA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017040">
        <property type="term" value="F:N-acylsphingosine amidohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046514">
        <property type="term" value="P:ceramide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042759">
        <property type="term" value="P:long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046512">
        <property type="term" value="P:sphingosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2300">
        <property type="entry name" value="Neutral/alkaline non-lysosomal ceramidase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006823">
        <property type="entry name" value="Ceramidase_alk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038445">
        <property type="entry name" value="NCDase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031331">
        <property type="entry name" value="NEUT/ALK_ceramidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031329">
        <property type="entry name" value="NEUT/ALK_ceramidase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12670">
        <property type="entry name" value="CERAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12670:SF1">
        <property type="entry name" value="NEUTRAL CERAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04734">
        <property type="entry name" value="Ceramidase_alk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17048">
        <property type="entry name" value="Ceramidse_alk_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Neutral/alkaline non-lysosomal ceramidase N-terminal" evidence="6">
        <location>
            <begin position="7"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="Neutral/alkaline non-lysosomal ceramidase C-terminal" evidence="7">
        <location>
            <begin position="288"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="23"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR606823-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR606823-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU366019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ83590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="463" mass="50379" checksum="C37CD63C8B4EA36A" modified="2010-06-15" version="1">MLEKDIGGSFVAGFSQSNVGDTSPNTEGPICQDTGLPCKYEDSTCGGKTQQCMGRGPAFRISDTESCRIIGEKQYLGAKAIYHGTGKAPVAGDGGAVRSFHTFVDFSNYTFQLSNSTTKRTCKAALGFSFAAGTTDGPGAFDFTQNDPNAPSNPFWLLVRNFLRKPSKDQVECHQPKPILLDVGEMDKPYPWAPNIVDVQMFRVGQLAIIISPGEATTMSGRRWKKAVAEELNNTGIISSGDSWIVLGGPANSYTHYIATPEEYAIQRYEGASTLYGQFTLGAYISLYKKYIPYLGATPPSTPVPPGPSPPINTNTSISLIKGVVHDNPRLGKNFGDVLTDVASTPYSSGSLVKVVFVGANPRNNLRLGGTYAAVERNNGGTWSRVRDDTDWDLVYQWERTEGFSGQSEVTISWTIERGTPAGRYRIKYYGDSKRPFTGKITAFEGTSRTFNIGPRRTTYSIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGF3</accession>
    <name>D5GGF3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007353001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430264">
        <property type="protein sequence ID" value="CAZ83596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839405.1">
        <property type="nucleotide sequence ID" value="XM_002839359.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGF3"/>
    <dbReference type="GeneID" id="9183194"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007353001"/>
    <dbReference type="eggNOG" id="ENOG502SESG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2198510_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGF3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990879">
        <property type="term" value="C:CST complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043047">
        <property type="term" value="F:single-stranded telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016233">
        <property type="term" value="P:telomere capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024222">
        <property type="entry name" value="Ten1_fungal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12658">
        <property type="entry name" value="Ten1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="129" mass="13745" checksum="CB9F2563ACF803FE" modified="2010-06-15" version="1">MEPSSSSLPPYTQPGSKLLPLDQIPSCPPNTRVRFLGCIASYDMTTAELSLSRPPSSVFIKTDVSLLTSSLSMGSLRDGEWVNVIGDTQKEEKGEGVLVMAITLWSAGGINLGKYEKGVKGRLEADALG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGI1</accession>
    <name>D5GGI1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007398001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430264">
        <property type="protein sequence ID" value="CAZ83624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839433.1">
        <property type="nucleotide sequence ID" value="XM_002839387.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGI1"/>
    <dbReference type="GeneID" id="9185546"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007398001"/>
    <dbReference type="HOGENOM" id="CLU_2470710_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGI1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="88" mass="10021" checksum="85525A6AFDA70F21" modified="2010-06-15" version="1">MYRHNIIRCGIGVGLGWGRYVGERGRGVLCFALPFTFIVVLLLLVVVETSHEPRLREFFPLLPFVERLPLPVPSSNPFTILHTSKVKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGK9</accession>
    <name>D5GGK9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00007542001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430275">
        <property type="protein sequence ID" value="CAZ83739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839548.1">
        <property type="nucleotide sequence ID" value="XM_002839502.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGK9"/>
    <dbReference type="STRING" id="656061.D5GGK9"/>
    <dbReference type="GeneID" id="9181709"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007542001"/>
    <dbReference type="eggNOG" id="KOG0032">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_63_0_1"/>
    <dbReference type="InParanoid" id="D5GGK9"/>
    <dbReference type="OMA" id="RPKVQPC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004683">
        <property type="term" value="F:calcium/calmodulin-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071277">
        <property type="term" value="P:cellular response to calcium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106057">
        <property type="term" value="P:negative regulation of calcineurin-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010972">
        <property type="term" value="P:negative regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000153">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase type I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="21"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ83739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="347" mass="39077" checksum="62F93423ECA01E0E" modified="2010-06-15" version="1">MSAVPENSSAPQKPQVQPCRYKTGKTLGSGSYSVVKECVHIDTGRYYAAKVINKRLMVGREHMVRNEIAVLKKVSMGHQNILTLVDYFETMNNRNLPCHRSCSRRRISDASDLIRATLSAVAYLHDHGIVHRDLKPENLLFRTAEDNSDLLIADFGLSRIMDEEQFHILTTTCGTPGYMAPEIFNKTGHGKPVDVWAIGVITYFLLCGYTPFDRDNHVDEVTAISQADYKFEPEDYWKDVSETARDFIRGCLTIDPSARMTCHKALEHPFLVEGYRGNDLLPVVRKNFNARKTLHAAIDTVRAIHKLREGGTMDGALSISPESQKEKEIEKQTVGLWRKPAGGVMKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGN2</accession>
    <name>D5GGN2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007433001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430275">
        <property type="protein sequence ID" value="CAZ83654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839463.1">
        <property type="nucleotide sequence ID" value="XM_002839417.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGN2"/>
    <dbReference type="STRING" id="656061.D5GGN2"/>
    <dbReference type="GeneID" id="9185576"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007433001"/>
    <dbReference type="eggNOG" id="ENOG502SSQD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1023751_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGN2"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056125">
        <property type="entry name" value="DUF7708"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24809">
        <property type="entry name" value="DUF7708"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7708" evidence="1">
        <location>
            <begin position="26"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="272" mass="31169" checksum="E65B6F4E3040EA2C" modified="2010-06-15" version="1">MDDVLKYTSEAMESWENKRCWKKPRKWLECFSTRVTYYGKILDVIAQHHPEYVSLAWGTMKLLFVIVINHETSVKQVAKTLVRIAESLSQVVPLLHLYPNGEMINAVASLYAHMISFLQRATIWYRGSSTSRLFKAIFSPFELSFGELLVEIREHGEHVAKLADVGHKQETREIKELLYENVKRPGVGIDTGQELDARRGMQGQAYYTQMMPDGFFSICGMTLSVFAKGKLEEPPLWLTLLILFVRHKRAAKIDFCLANGIRKSFAELCSDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGQ6</accession>
    <name>D5GGQ6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="11">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00007468001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430275">
        <property type="protein sequence ID" value="CAZ83678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839487.1">
        <property type="nucleotide sequence ID" value="XM_002839441.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGQ6"/>
    <dbReference type="STRING" id="656061.D5GGQ6"/>
    <dbReference type="GeneID" id="9185600"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007468001"/>
    <dbReference type="eggNOG" id="ENOG502S1JA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048915_2_0_1"/>
    <dbReference type="InParanoid" id="D5GGQ6"/>
    <dbReference type="OMA" id="HYVPKTF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030003">
        <property type="term" value="P:intracellular monoatomic cation homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033122">
        <property type="entry name" value="LETM1-like_RBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044202">
        <property type="entry name" value="LETM1/MDM38-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14009:SF6">
        <property type="entry name" value="LETM1 RBD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14009">
        <property type="entry name" value="LEUCINE ZIPPER-EF-HAND CONTAINING TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07766">
        <property type="entry name" value="LETM1_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51758">
        <property type="entry name" value="LETM1_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="146"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="Letm1 RBD" evidence="10">
        <location>
            <begin position="140"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="333"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="338"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ83678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="360" mass="40324" checksum="2DF11CF45DCC27C2" modified="2010-06-15" version="1">MSIAIRPILQNTCRTLVLFPRARANSTSAAAAKTHGGGAAGGGEHKVLDPEFVNSPLSTLPAALELPQKAPGDSTFPYLIKTGKAYLGFYKSGLKNVYKNFIATRPIQSRIDEKGSITDLVGAGGITRREFQLVMRSRHDSRRIPIFGLVFIIFGEMSPFVLPFISGIVPFNCRIPRQIHRDRTKLEERRAATFRNLPRGRDLLELAREKRSVRDLTSNEVLHCSTVLGLHSGKWPSVLYPFLPPMMWLRFKLWRRLMYLELDDTLLARGGGPGMLDSGEELKMAAVDRGFDTLQKTETRLREELKLWLKAKETAGGMTPALWLTRPIEWRNSFPPSTSSPPPSSTTPPPPRTPTPRKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHF2</accession>
    <name>D5GHF2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00007908001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430318">
        <property type="protein sequence ID" value="CAZ83945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839754.1">
        <property type="nucleotide sequence ID" value="XM_002839708.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHF2"/>
    <dbReference type="STRING" id="656061.D5GHF2"/>
    <dbReference type="GeneID" id="9183620"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007908001"/>
    <dbReference type="HOGENOM" id="CLU_1511685_0_0_1"/>
    <dbReference type="InParanoid" id="D5GHF2"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ83945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="178" mass="19485" checksum="02C724CFFAB7D39F" modified="2010-06-15" version="1">MADIPEQTDSQILPQPGMESVSQVAEILALLQQLQQGQAALHEGQVSLQQAQTALQEGQAALQQGQTDFRGEQAALLEGQADILQQVDELRDEGSRQDKNSIARVINNNARLDDTLLEPFYGINGQLVPHFPPTLGHAKRLRSDRVDALLLALGLGVAGSLAVRQTRFKRYIGLRDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHN4</accession>
    <name>D5GHN4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00008058001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 13 family.</text>
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    <dbReference type="eggNOG" id="KOG0471">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="HOGENOM" id="CLU_006462_1_2_1"/>
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    <dbReference type="OMA" id="TENEWFT"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="term" value="F:alpha-amylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:glucan 1,4-alpha-maltotriohydrolase activity"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0004574">
        <property type="term" value="F:oligo-1,6-glucosidase activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004575">
        <property type="term" value="F:sucrose alpha-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000025">
        <property type="term" value="P:maltose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:sucrose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11333">
        <property type="entry name" value="AmyAc_SI_OligoGlu_DGase"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.400.10">
        <property type="entry name" value="Oligo-1,6-glucosidase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="GH13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045857">
        <property type="entry name" value="O16G_dom_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10357">
        <property type="entry name" value="ALPHA-AMYLASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10357:SF232">
        <property type="entry name" value="GLYCOSYL HYDROLASE FAMILY 13 CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0462">Maltose metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 13 catalytic" evidence="5">
        <location>
            <begin position="29"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00026248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ84064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="625" mass="72115" checksum="5D481D1809483F12" modified="2010-06-15" version="1">MTTLVGETKKGQESERSKRAWWKHAVVYQIYPSSFNDSNGDGIGDIPGIIEKLDYLKKLGIDVLWLSPIYCSPMKDMGYDISDYRRVDPRYGTLEDVETLVKEARKRSIKIVMDLVVNHSSDEHGWFKESRKSKTNPKRDYYIWRQPKFDAKGKRLPPNNWVSVFGGSAWTYDETTGEYYLHLFTSSQPDLNWESPVLRREVYSIMSFWLERRIAGFRMDVINLISKTPELPDAPVTDPKEECQDGSMYFANGPRLHEFLQEMRREVLDKFDTITIGEMPFVTDPQEVLKCVKGRRELDMVFQFDIVEVDSVPGQKKFYPHRWKLADLKAVVDKWQTFMINNDGWNAVYDENHDQPRSVFRYVDRSVVERSFQAREMAAKMLATFLVLQSGTLFLYQGQEIGMGNVPREWGIEEYKDVEGLNFWNKLLEEREGDLSKMKDAMEALQSKARDNARTPVQWDSSPQAGFTTGSPWMRVNDDYEEWNVAAQIDSPSSVYNYYTKLLGLRRDHKNIIVYGGFEMLSHSHEKVFAYKRYGYHGPDVGKAAVVVVMNFTGEAEVEWDLKEGKFGKEGLLQKPEVIIGTYGDGKVTVKDGIVRLRPFEAVVFWGDFVDFSRGGGQGPNCVVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHN8</accession>
    <name>D5GHN8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00008063001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430320">
        <property type="protein sequence ID" value="CAZ84068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHN8"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008063001"/>
    <dbReference type="HOGENOM" id="CLU_1950381_0_0_1"/>
    <dbReference type="InParanoid" id="D5GHN8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="91"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ84068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="129" mass="13570" checksum="6BB9684BA0D90818" modified="2010-06-15" version="1">MKRGVIVGGTEMPNSASIRSSGYSSSSGGKSVLLPRLCRGGVKAGTVRKGSFLPGLELGLLGAGGGVLGVVTLGGIVSGETDSSAIVANTVEIAFCLESSIFKLWAVALWRFWRWWRTSQIYNYISNAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHT9</accession>
    <name>D5GHT9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00008098001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430321">
        <property type="protein sequence ID" value="CAZ84082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839891.1">
        <property type="nucleotide sequence ID" value="XM_002839845.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHT9"/>
    <dbReference type="GeneID" id="9184175"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008098001"/>
    <dbReference type="HOGENOM" id="CLU_3351401_0_0_1"/>
    <dbReference type="InParanoid" id="D5GHT9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ84082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="37" mass="4631" checksum="CE5FC44E859DACF7" modified="2010-06-15" version="1">MRMRSRVIFFHGFGRVRFCLPREVWIKKSRLVVFVLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHV7</accession>
    <name>D5GHV7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008137001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430321">
        <property type="protein sequence ID" value="CAZ84100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839909.1">
        <property type="nucleotide sequence ID" value="XM_002839863.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHV7"/>
    <dbReference type="GeneID" id="9184193"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008137001"/>
    <dbReference type="eggNOG" id="ENOG502QW3V">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_459416_0_0_1"/>
    <dbReference type="InParanoid" id="D5GHV7"/>
    <dbReference type="OMA" id="HEPRIEH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="226"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="265"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="317"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="351"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="404"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="265"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="276"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="355"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="424"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="434"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="445"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="499"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="549"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="594" mass="65096" checksum="6715CF30FC4C52E6" modified="2010-06-15" version="1">MPVLFDEEMLIPPSMRRHRRYYTNTYDDSRVETTVIESPSMASTTTIELPSHRSLTPPHSPVLIHEDHYVPLPTEIECSRGREYVIHPAGRPAVYHKRIGGGFGGELDMDYSHGHGYRHGRHYHRARSAGPVSDYDSYSDDYYLDDGTRHGIRHHRHGSPHGHHGRHGHRMEHLLEGGAGIVGAHNPHEHREHKGHFHHRSSSSDGHKGRHLLETAGLALGAKKLAGMRHRSHSRPGRSPSGGRRRHLAEAGIAAVGLDHALNHRRSRSHSRHRHFSSDSERHPGWGRDDHKAHRRAKRLAEAGLGVAAAGAAKELYDHRKHHRSSSASSSGGERNHNGRHIAAAALGATAAGLAHHKRSHRRRSSSISSYSSSSDDENKHRGRKIAGAAVGTAATGLAAKALYDHRRNRSRSSSTSSSLSGGGKHRRRHRLGKAALGTAAAAGLKHHRDRRSRSRSRSSSRERRNHANGLRKAGYTMAGALAERHRREQKHGRSLSADGRRPDGHHNGYHDGHHITHGHLKPGQGFVESESIHSSPSRTGNRPRRGSHGGGILGGGPGPSATRNIGEAVAYKLGEAVAEGLNRGRSGSMGHRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>D5GI03</accession>
    <name>D5GI03_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008215001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430322">
        <property type="protein sequence ID" value="CAZ84146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839955.1">
        <property type="nucleotide sequence ID" value="XM_002839909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GI03"/>
    <dbReference type="STRING" id="656061.D5GI03"/>
    <dbReference type="GeneID" id="9184231"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008215001"/>
    <dbReference type="eggNOG" id="ENOG502QU5B">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1262338_0_0_1"/>
    <dbReference type="InParanoid" id="D5GI03"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070772">
        <property type="term" value="C:PAS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010513">
        <property type="term" value="P:positive regulation of phosphatidylinositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903778">
        <property type="term" value="P:protein localization to vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000011">
        <property type="term" value="P:vacuole inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024260">
        <property type="entry name" value="Vac7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28258">
        <property type="entry name" value="VACUOLAR SEGREGATION PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28258:SF1">
        <property type="entry name" value="VACUOLAR SEGREGATION PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12751">
        <property type="entry name" value="Vac7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="8"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="142"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="162"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="219" mass="23063" checksum="F3E8CA15E477C11D" modified="2010-06-15" version="1">MRVETETVSSVPQVAVGAAGVPGGAGIRSENSNDTIRAPKKDKKKPTKRVGGGGPANPWSKADLFARRVAEAVDEANSSDSEETFVYESNPPEAPQKPTKRFHSRTPSATSIQGGPDRGLRLPLLGSMDGNQSAGRNGMKFANTSGASNTNAASDGATEKQAAIEREEEGDRGHRQLLGELVAQGRRDSQAPDKRKRAGIPGVRMGIGLCSRVSVFIVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIE0</accession>
    <name>D5GIE0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008441001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430326">
        <property type="protein sequence ID" value="CAZ84283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIE0"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008441001"/>
    <dbReference type="HOGENOM" id="CLU_2795790_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIE0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="68" mass="7345" checksum="801322F4A26DB3E7" modified="2010-06-15" version="1">MSRASQQDNSKRPASPLPETRPTHGVRIGNNNRNCGNIDSSYNTINYIGNTKNFGQGGVVIFNGATVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIF3</accession>
    <name>D5GIF3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00008469001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430326">
        <property type="protein sequence ID" value="CAZ84296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIF3"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008469001"/>
    <dbReference type="HOGENOM" id="CLU_2795791_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIF3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ84296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="68" mass="7212" checksum="6F7F1A976AD35A6A" modified="2010-06-15" version="1">MWIRCLASSPIVVRKLVASPPGVFGDDEGNGAGKRPDAICRQWVYGSSRGQVGDFHGEDLGSHTLLSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIU3</accession>
    <name>D5GIU3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00008690001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840245.1">
        <property type="nucleotide sequence ID" value="XM_002840199.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIU3"/>
    <dbReference type="STRING" id="656061.D5GIU3"/>
    <dbReference type="GeneID" id="9185971"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008690001"/>
    <dbReference type="eggNOG" id="ENOG502RY0W">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026939_1_0_1"/>
    <dbReference type="InParanoid" id="D5GIU3"/>
    <dbReference type="OMA" id="LGPKFMW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053247">
        <property type="entry name" value="GPCR_GPR1/git3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42058:SF1">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 2 PROFILE 2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42058">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="127"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="165"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="208"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="252"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="321"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="400"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="7">
        <location>
            <begin position="90"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="499"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="546"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="559"/>
            <end position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ84436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="590" mass="64993" checksum="C7B883CD12F1D46A" modified="2010-06-15" version="1">MWGARGGAGLLRHICVRLVNRRVFFPTESEEWLPITNGMAASCPSPFVPGSDYPESGGDTNARICLPMISGGGTCCLPCPRQQWVYPDNFQHLVRYSTNVNIVGVGCCVFMLLSFAILPVECTHRHYLSVCLVLAIMLMQVSFIIPMGSSSTQCFDKITPHDMYSSINCALSGAFLLGGGWCTVMWVFLRTLSLHLQICWQVIPGRRFFLTAQAAGWSIPVVFLAVALSITGVSFRFGESCHINSKAGLQTFWGPLLAVAAASIVTQSITFGYCIQVYLRSLMEEKEATELRSPLPYATSVRTVTAGQAFKRIQRVVVLQWRGIFVVVLILADVVFFATVFLQFDGTTEKTPENVQAGFKWLMCLLQNEGDKGKCLEVAAELVIPEATALAVLFLLSFNGIWALILLGNISIFVGWYNFFKNILVRKKPNDEFVSFNARRPPDDPSYEMLDSVKSPSTPGCFHPAVLKPGQDTSLSLSMPREYNPNTFSALLRGRADSDAYSHPPRLRSESSFSSPPYTPFKDTEATGRTPPAPYSPSKEFDSAGRNPSLSGGQFSPYQDHRADYKSEASDYVSSQPPQYPPDAKIGVAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJ45</accession>
    <name>D5GJ45_TUBMM</name>
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        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00008834001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430329">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="GeneID" id="9181879"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008834001"/>
    <dbReference type="HOGENOM" id="CLU_3160286_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJ45"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ84538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="48" mass="5062" checksum="230A9AB808E066A2" modified="2010-06-15" version="1">MSGGFGMTSTPPFTSKTWECQSLRSSAGSDSVVDYALADDAPSVMWAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJ65</accession>
    <name>D5GJ65_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00008860001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
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            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="CAZ84558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_002840321.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJ65"/>
    <dbReference type="STRING" id="656061.D5GJ65"/>
    <dbReference type="GeneID" id="9185926"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008860001"/>
    <dbReference type="eggNOG" id="ENOG502S64Q">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054960_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJ65"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053065">
        <property type="entry name" value="Archenteron_Induction-Rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024382">
        <property type="entry name" value="Vps3844_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36853:SF1">
        <property type="entry name" value="DUF3844 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36853">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12955">
        <property type="entry name" value="Vps3844_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072183" evidence="3">
        <location>
            <begin position="19"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="368"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar sorting protein Vps3844 C-terminal" evidence="4">
        <location>
            <begin position="309"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="277"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ84558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="411" mass="44338" checksum="1E8410CC24A3456E" modified="2010-06-15" version="1">MRVLPGLVVSCFAGVAASSTVYELYHDHSDDATSVSPEELALSLSERLGVSRYYELGPQGNPPRLDLDGGKSRMFRARIEEDFVLSIAGAYGIGATQPESTQIGLRDMPKASAFGELMDVFVRKNRQFLEAAVPRVVDMSPWGGMCVGVSENEISLDPKEYTPTIEVSEDDYGTPSEAANLLREYGIIKKITQRDIRSLPGQHLASCHVAGVEKYVKQYGPESKQALNAAQLYLSLIDDIPAHIRSTILLLPATKSNTGVSAFKIDLHKAYKRSEEEPLSFTAHSPPPAEATSASSENPKRILGPIPRCYSSQNSCTNATNACSGRGECVKSSFPTDCWACSCKGPDGKKMDKWAGAACQKEDISVPFNLFLVLGIAIIFVSIWAVGLLYSIGDEPLPSVLSAGVAPAKRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJA8</accession>
    <name>D5GJA8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00008915001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the purine-cytosine permease (2.A.39) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840410.1">
        <property type="nucleotide sequence ID" value="XM_002840364.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJA8"/>
    <dbReference type="FunCoup" id="D5GJA8">
        <property type="interactions" value="559"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJA8"/>
    <dbReference type="GeneID" id="9185969"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008915001"/>
    <dbReference type="eggNOG" id="KOG2466">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021555_4_0_1"/>
    <dbReference type="InParanoid" id="D5GJA8"/>
    <dbReference type="OMA" id="LPLLWIH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015205">
        <property type="term" value="F:nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4160.10">
        <property type="entry name" value="Hydantoin permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001248">
        <property type="entry name" value="Pur-cyt_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045225">
        <property type="entry name" value="Uracil/uridine/allantoin_perm"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30618">
        <property type="entry name" value="NCS1 FAMILY PURINE/PYRIMIDINE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30618:SF0">
        <property type="entry name" value="PURINE-URACIL PERMEASE NCS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02133">
        <property type="entry name" value="Transp_cyt_pur"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="178"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="206"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="249"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ84601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="352" mass="38654" checksum="EB332115668E12F1" modified="2010-06-15" version="1">MLFSYSLTRKRVSPYFHLRLPYNAGAYGTARWSNKDLDPVPVELRTWGGLDYWAYWCSDMLAPPLAATVSTVMSLGFTARETIPIVFFGFMICSIVVTLTGKMGATYHVAYPVIVRSTFGMIGSYPAIVIRAFVAMMWTAILCVQAGGFLQNCIEAIWPSFKNFPNHLSEGAGIDCRSISAGLLCFFLYWILQTILSLMPIQKLRLLFLAKGIIVPPTFLALFLWAVIVTDGGGPLVTGKYVLETPMNTAYSSLTGLNVMIGLFSSMAVNMPDFGRFSKNNLAGGHQFFALPIIGTLGALTPIFVNSAHKKIWGEFEWYMPAVIAKFDSRAAKFFTGASFILATIGNQIAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJF9</accession>
    <name>D5GJF9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00008980001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430330">
        <property type="protein sequence ID" value="CAZ84652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840461.1">
        <property type="nucleotide sequence ID" value="XM_002840415.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJF9"/>
    <dbReference type="GeneID" id="9182653"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008980001"/>
    <dbReference type="HOGENOM" id="CLU_2777737_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJF9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ84652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="69" mass="7418" checksum="256F573B9EF1CF5A" modified="2010-06-15" version="1">MAERNTTAHKVTGGDVVAILLYIAGRNCAQFSSGDSGLSREYLRTTGQMQLSNRGCLNFAIVPIANLMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJR8</accession>
    <name>D5GJR8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00009161001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the metallo-dependent hydrolases superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430333">
        <property type="protein sequence ID" value="CAZ84761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840570.1">
        <property type="nucleotide sequence ID" value="XM_002840524.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJR8"/>
    <dbReference type="STRING" id="656061.D5GJR8"/>
    <dbReference type="GeneID" id="9186796"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009161001"/>
    <dbReference type="eggNOG" id="KOG4245">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039329_2_1_1"/>
    <dbReference type="InParanoid" id="D5GJR8"/>
    <dbReference type="OMA" id="RTNDWIV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019748">
        <property type="term" value="P:secondary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032465">
        <property type="entry name" value="ACMSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21240">
        <property type="entry name" value="2-AMINO-3-CARBOXYLMUCONATE-6-SEMIALDEHYDE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21240:SF28">
        <property type="entry name" value="ISO-OROTATE DECARBOXYLASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04909">
        <property type="entry name" value="Amidohydro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="2 3" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="4">
        <location>
            <begin position="13"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU366045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ84761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="340" mass="38192" checksum="6EA610080E90C5B3" modified="2010-06-15" version="1">MNASTAFIGPGRIDTHHHYFGDSVPELVSEFTNDGAQAFFFPKGVDDHLKYMDSMGIQTAVLAPSIKMEWHTQWPPARWKNLCERTLKAQLKVQESNPLRFGTFAMLPFPHVNETLDFVRDIYTREVKPDGFALSTAMGNKYLGDPAFDPMWDELNMHSAAIFVHPADTAMPPGLEYKPYVFEYAFDTARAMASTIINGVFTRYPNLKFIFSHNGGAFPFMAKRLDDVGPSAKKMNNGKSVFEVIRTSNIFVDTAISAPIQWPVTLDIGFPVERIIYASDYPYTAAVGEVAYHGKTAPEESGQFTRHELDVKIARENALKGLFPRLAVEYKKAFGHKVDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJV1</accession>
    <name>D5GJV1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00009210001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430334">
        <property type="protein sequence ID" value="CAZ84794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840603.1">
        <property type="nucleotide sequence ID" value="XM_002840557.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJV1"/>
    <dbReference type="GeneID" id="9186830"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009210001"/>
    <dbReference type="HOGENOM" id="CLU_475025_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJV1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="77"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="389"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="462"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="532"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="298"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="308"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="330"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="462"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="553"/>
            <end position="574"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ84794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="574" mass="63553" checksum="9803072EF0CDE0BD" modified="2010-06-15" version="1">MDHMEPASIEFHSYQQSSSPPPSSILSSFTFQKLSETVFIILLNILKMFWNTMFQPLPYYTAPTTPNSRLLTPLVYLYWAVMCCVLLPLAPIYLFTSLMGTIYISTQMGWRLKQTKPEVIEDIKKRYLAFVPATPVPEHTPSAEPAPVTESPPVTESAPVAEANPIVVEPVQTVVVEGVVEEKQKGEDDAAIVPHTVVEEPEAEEDVDSAIEPALRVEEESPEFILPVHEEVQTETPIRETHEETPQVLAKQESLTVGEYSSPIVAGSDSEDVEESCGMESKYTESVPSDYSGSASSDIEESDEDELGEKKHNEGRGLEGIVEEEEADEEILRKDLDEREVSDQELTPQAPEYIPPAHRVKLEEPVFVRSYGPPAFGICEKRGVREIVDPRKRHSHQARIQQPVSLRMEESLASSDESSPYDDESERDGSPPISPISSVGTGNWGVIIESLDKIVELDIGADKAESPKDDVDTSVTPAPLDPQKLEAPQVTEDVSPCVAPVQLVPTTMGQEKQAAIVSEQTLMPVVEIAPKLERSNTDDSGFESGSVKEVKVQKKKKKAPMKQRLGNRKSKRSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>D5GK14</accession>
    <name>D5GK14_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00009313001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430335">
        <property type="protein sequence ID" value="CAZ84857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840666.1">
        <property type="nucleotide sequence ID" value="XM_002840620.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GK14"/>
    <dbReference type="GeneID" id="9186894"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009313001"/>
    <dbReference type="HOGENOM" id="CLU_1908221_0_0_1"/>
    <dbReference type="InParanoid" id="D5GK14"/>
    <dbReference type="OMA" id="KRRMEIP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031501">
        <property type="term" value="C:mannosyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000030">
        <property type="term" value="F:mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019433">
        <property type="entry name" value="GPI_ManTrfase_II_coact_Pga1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28022">
        <property type="entry name" value="GPI MANNOSYLTRANSFERASE 2 SUBUNIT PGA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28022:SF1">
        <property type="entry name" value="GPI MANNOSYLTRANSFERASE 2 SUBUNIT PGA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="133" mass="14860" checksum="B0414B380D5B5BAE" modified="2010-06-15" version="1">MDLTLNIYTAPQLLDKPSLLANVTAYATRQLSFSPANELSGPSAAPRSVLYLDIRAKAGYYTHEKRRMEIPDPVEVEITVDPFVLNVLPESLLPIAVMVILTTLSAFWTSGRVYSALRDIASLKQSRYDMKTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>D5GK31</accession>
    <name>D5GK31_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histone H2A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00009338001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histone H2A family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430335">
        <property type="protein sequence ID" value="CAZ84874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840683.1">
        <property type="nucleotide sequence ID" value="XM_002840637.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GK31"/>
    <dbReference type="FunCoup" id="D5GK31">
        <property type="interactions" value="976"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GK31"/>
    <dbReference type="GeneID" id="9186911"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009338001"/>
    <dbReference type="eggNOG" id="KOG1757">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062828_2_1_1"/>
    <dbReference type="InParanoid" id="D5GK31"/>
    <dbReference type="OMA" id="MNKKGAP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000791">
        <property type="term" value="C:euchromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140898">
        <property type="term" value="P:CENP-A eviction from euchromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070481">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, non-stop decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030466">
        <property type="term" value="P:silent mating-type cassette heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00074">
        <property type="entry name" value="HFD_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002119">
        <property type="entry name" value="Histone_H2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032454">
        <property type="entry name" value="Histone_H2A_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23430">
        <property type="entry name" value="HISTONE H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16211">
        <property type="entry name" value="Histone_H2A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00620">
        <property type="entry name" value="HISTONEH2A"/>
    </dbReference>
    <dbReference type="SMART" id="SM00414">
        <property type="entry name" value="H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0158">Chromosome</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histone H2A C-terminal" evidence="4">
        <location>
            <begin position="85"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ84874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="123" mass="13247" checksum="DFFB6E59BC8C7EAA" modified="2010-06-15" version="1">MSGGKGKSHGGKARPKGASDGARQQVSHSAKAGLQFPCGRVKRFLKNNTQNKMRVGAKAAVYVTADLKVKRITPRHLQLAIRGDEELDTLIRATIAFGGVLPHINRALLLKVEKKKASAPKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>D5GK36</accession>
    <name>D5GK36_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00009345001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430335">
        <property type="protein sequence ID" value="CAZ84879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840688.1">
        <property type="nucleotide sequence ID" value="XM_002840642.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GK36"/>
    <dbReference type="STRING" id="656061.D5GK36"/>
    <dbReference type="GeneID" id="9186916"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009345001"/>
    <dbReference type="eggNOG" id="ENOG502RWR6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_532305_0_0_1"/>
    <dbReference type="InParanoid" id="D5GK36"/>
    <dbReference type="OMA" id="HNRHLNM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="467"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="467"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="484"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="512" mass="56306" checksum="B29B21C6313A7BB0" modified="2010-06-15" version="1">MPKAPSTRRNLTTNQPSRKPPTSSKPPPRPKKTTIYNPNKHSLASLPYHILTTILHYAASTPNSPFPDTTLLLKTLTLCRTLYEPTLAVLYHTPPTNTLPCAHRLLRTLRERVHVRPLVKALIADIEPLLVTRAAPWGAWDIREFLSLASGVREVDIRHARVSSVRAGAMGRSWRYPSELWNVLDQAGVLLHGWTWRGEFMERIDLDELKRVHSLRPFSGLRRVGLVGIESNDEGATAGDLSREEGCAEVLASLGALREVAFEDCSVANEHFSSELTTRAPGARLTKLSFTNCPRLTSSEDNLPSLLQSPLCSTTLTSLTITNSPSCTLSFTLFLPPTLQTLSYTASSIPSPLSILPIPPTPTQWPQDLTSLTLNLIKFTSQTACSALLSSLISAAPRLSAMHDIAIWCILDMDWRARGTFRETWEPRVKSAFQRCWTGGSGGEVRFDNSRPAERIFSERDFLDFGNGAGGEGGGGRRNRASRGTGGGRRSGGVGILGVRRTAESDDEDYTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKB0</accession>
    <name>D5GKB0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00009454001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the YOS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430337">
        <property type="protein sequence ID" value="CAZ84953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840762.1">
        <property type="nucleotide sequence ID" value="XM_002840716.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKB0"/>
    <dbReference type="FunCoup" id="D5GKB0">
        <property type="interactions" value="254"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GKB0"/>
    <dbReference type="GeneID" id="9181973"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009454001"/>
    <dbReference type="eggNOG" id="KOG4779">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_152125_0_1_1"/>
    <dbReference type="InParanoid" id="D5GKB0"/>
    <dbReference type="OMA" id="VQTVMRM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013880">
        <property type="entry name" value="Yos1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15858">
        <property type="entry name" value="IMMEDIATE EARLY RESPONSE 3-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15858:SF0">
        <property type="entry name" value="IMMEDIATE EARLY RESPONSE 3-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08571">
        <property type="entry name" value="Yos1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072803" evidence="8">
        <location>
            <begin position="24"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ84953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="90" mass="10038" checksum="A8D218ED94CCF91A" modified="2010-06-15" version="1">MFFGLMPLIKMTILMINAIAILSEDRFLARIGWSTATPIEPQSFGMQQQIVANESIKAKTMNLISATRTLMRIPLIGINSLLIVYELLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKB4</accession>
    <name>D5GKB4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00009460001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAK10 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430337">
        <property type="protein sequence ID" value="CAZ84957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840766.1">
        <property type="nucleotide sequence ID" value="XM_002840720.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKB4"/>
    <dbReference type="FunCoup" id="D5GKB4">
        <property type="interactions" value="587"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GKB4"/>
    <dbReference type="GeneID" id="9181977"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009460001"/>
    <dbReference type="eggNOG" id="KOG2343">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011757_0_0_1"/>
    <dbReference type="InParanoid" id="D5GKB4"/>
    <dbReference type="OMA" id="QMEWIVQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031417">
        <property type="term" value="C:NatC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057983">
        <property type="entry name" value="NAA35-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007244">
        <property type="entry name" value="Naa35_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057982">
        <property type="entry name" value="TPR_NAA35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21373">
        <property type="entry name" value="GLUCOSE REPRESSIBLE PROTEIN MAK10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21373:SF0">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 35, NATC AUXILIARY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04112">
        <property type="entry name" value="Mak10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25789">
        <property type="entry name" value="TPR_NAA35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAA35-like N-terminal" evidence="4">
        <location>
            <begin position="68"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="NAA35-like TPR repeats" evidence="5">
        <location>
            <begin position="298"/>
            <end position="702"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ84957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="712" mass="81110" checksum="7608CC54A45F49AA" modified="2010-06-15" version="1">MGEAEDNGVNHLAYPAEDFERLTIKEKGPNPTPAPTTNPITPVPQATMDSFRDVTAEFFEASEMLEIGQLVKDPYFTLLQAVGALEIMDPKMDSGMLRAEYESFDALQPRLPEEVVGIMDQLLCHEMAWNRGSALSQTLFTSLYIDRLLSSGPSSLAEATFRGPHGKKTDTPEAQLVENVLQPFCVGLIKCCSHVNHQILAEQIYEEEDFVTQVYGVNLFEGVKSEEIVALLESALSWLEGTGDLYSEGIRDAFKHRLLLRKSLVLVFSSETKEWSAETTEIWDTSLEHVKGVEETYKILNFEGSTNLVNYFLGFTTRQPAPSPYIRSLLQSVFFGQVENPGKFSIKQLLYDDLKELCYPADILLDPQNDLVEAPQDPRFEITKHMSWFVERAGKSFMELFRNICQNRPRLRRTLCHSILDWDSLQVEAEEVDSELRQFTNEEAVMTPNGPSFAFPLSSWVYHYKLRQMEWIMLLGFELDVFQTHEFAGMYWYLQHYTRTRISHIERMLQFASRAISGPPLERRKELRSNPQQVKTLSLLNFYLLEAAGVQELAGAMIALFTALSRLDLLKKPPQPFSSDALRYEVRMKPFFTIGCPEVVPFEDFQSLASSQEIETSELLEMASESATDARKNFDQLCKLDARTARAVLCEERYRTSMREMMRSCIGVGVATAVLSKALQEGRATPELLDVQIDRERYHPSFSAPKIVAKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKB8</accession>
    <name>D5GKB8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00009467001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430337">
        <property type="protein sequence ID" value="CAZ84961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840770.1">
        <property type="nucleotide sequence ID" value="XM_002840724.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKB8"/>
    <dbReference type="STRING" id="656061.D5GKB8"/>
    <dbReference type="GeneID" id="9181981"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009467001"/>
    <dbReference type="HOGENOM" id="CLU_416897_0_0_1"/>
    <dbReference type="InParanoid" id="D5GKB8"/>
    <dbReference type="OMA" id="DIREWTI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="503"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="622"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="139"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="265"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="503"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="630"/>
            <end position="646"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ84961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="658" mass="74025" checksum="4211312E3F3E4285" modified="2010-06-15" version="1">MPNSFASIFQEAAYAFIKREDAREAHSKAEADRNKVAEYFNAFPVMRDRYEGRLENTRKELARANRGLEIKSRPLETICLEFARENCAKKELEPMVPFLRQLRHEFGTSDSLAQFLKSSLSPSTNLSQPQQSAAKPDELQRLSSELANLKKQQDDEAKLRRELSGDFREYKDNSSKRLKTCEDKLEDVETSQRDLRSELDQTKEEMRRVVDDIRGVQQSLAQLQESSTKNTQNSVPTATGANLSSDVAEIKRVAQETVLTLVSRCENLQEKYLQFETRVKELELKIDSIDASPVSSDAIARLEKQLKIQDETIDSIRQSAENDTEIMMEQFTTIDASIQENKFQTSQLSADLEEQKAVIRNGMGQDKNASEVQAILGEHRTNINTLKAELQNLITRQHDYASKLHDLDRNFSTITPKLSKEIEATQTKLAKWENSLDGCLTAVAHLDQKMSAFTTKDFYERVVSSLVAINPLLFNTTGQLQHMLNENKKVSTTVQRLLVEHQQNQASEPTQQQQQYQPRQGAPDPSSTNTEAANGTAKPGEAVPSEGPGSLSQALKDIESHTRALEELTRRVDQHIETYNGKMSDIREWTIIATKKLQENSDTCGDMAREVNLIQAFLNSANESAPPAGSGQNQSQWRAVSASTAPRNPGLESDVQSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKR0</accession>
    <name>D5GKR0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00009713001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430340">
        <property type="protein sequence ID" value="CAZ85103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840912.1">
        <property type="nucleotide sequence ID" value="XM_002840866.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKR0"/>
    <dbReference type="GeneID" id="9183339"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009713001"/>
    <dbReference type="eggNOG" id="ENOG502S7FQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084831_2_1_1"/>
    <dbReference type="InParanoid" id="D5GKR0"/>
    <dbReference type="OMA" id="MLRNEYR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035213">
        <property type="entry name" value="DUF5321"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17254">
        <property type="entry name" value="DUF5321"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="156"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="218" mass="25241" checksum="D4D91B7159ED1607" modified="2010-06-15" version="1">MTLQRLRPNLTRIINSKCVTYPLAQFSTVCPRLTQHPTPSRTRFLPRILDPAVWFPFYKTETRKGKAGSKERKPKIHNPATFFVVMAMLIGSQAIQMIRIRQTHEDYMWRADTKIAVLREVIERLEKGEDVDVEKVLGVGNEKEEKSWEDILKQLEEEDAEWAGERNQKAQPTPPSERSDNAATETSKPEEKPGENKRPREEPRKPRGPRSARDYGFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKU0</accession>
    <name>D5GKU0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="12">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00009768001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Positive regulator of amino acid starvation-induced autophagy.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Golgi apparatus</location>
            <location evidence="4">trans-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SCOC family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430341">
        <property type="protein sequence ID" value="CAZ85133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840942.1">
        <property type="nucleotide sequence ID" value="XM_002840896.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKU0"/>
    <dbReference type="STRING" id="656061.D5GKU0"/>
    <dbReference type="GeneID" id="9183369"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009768001"/>
    <dbReference type="eggNOG" id="ENOG502S4IZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102236_0_0_1"/>
    <dbReference type="InParanoid" id="D5GKU0"/>
    <dbReference type="OMA" id="MDHHATH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019357">
        <property type="entry name" value="SCOC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21614:SF0">
        <property type="entry name" value="GEO08385P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21614">
        <property type="entry name" value="SHORT COILED COIL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10224">
        <property type="entry name" value="DUF2205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="112"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="24"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="36"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ85133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="167" mass="18548" checksum="2E7765660BADF7B9" modified="2010-06-15" version="1">MSSARPPINRTYSSRSQVGVLESPTLSSASFTPDSIRGDQDPMDHHATHDARSRCSGDMVERGRSPVIEREVYGEDDARSMSPRRNSEELEKIGREAKATLEQQAKTLQSGLLALLDRVDKVREEHDKLEGENRFLQEYIGSLMATSKITGDSGAGTRNRNSTSRLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5GL77</accession>
    <name>D5GL77_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Spindle pole body component</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00010049001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasm</location>
            <location evidence="1 6">Cytoskeleton</location>
            <location evidence="1 6">Microtubule organizing center</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the TUBGCP family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430346">
        <property type="protein sequence ID" value="CAZ85270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841079.1">
        <property type="nucleotide sequence ID" value="XM_002841033.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GL77"/>
    <dbReference type="STRING" id="656061.D5GL77"/>
    <dbReference type="GeneID" id="9182064"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010049001"/>
    <dbReference type="eggNOG" id="KOG2065">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005595_0_0_1"/>
    <dbReference type="InParanoid" id="D5GL77"/>
    <dbReference type="OMA" id="QLSMWLL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000930">
        <property type="term" value="C:gamma-tubulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043015">
        <property type="term" value="F:gamma-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051011">
        <property type="term" value="F:microtubule minus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1900">
        <property type="entry name" value="Gamma-tubulin complex, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007259">
        <property type="entry name" value="GCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040457">
        <property type="entry name" value="GCP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042241">
        <property type="entry name" value="GCP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041470">
        <property type="entry name" value="GCP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302">
        <property type="entry name" value="GAMMA TUBULIN COMPLEX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302:SF27">
        <property type="entry name" value="GAMMA-TUBULIN COMPLEX COMPONENT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04130">
        <property type="entry name" value="GCP_C_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17681">
        <property type="entry name" value="GCP_N_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4 6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma tubulin complex component protein N-terminal" evidence="9">
        <location>
            <begin position="2"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="Gamma tubulin complex component C-terminal" evidence="8">
        <location>
            <begin position="313"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="558"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="570"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="584"/>
            <end position="601"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ85270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="732" mass="80652" checksum="769A76D20FD8CE81" modified="2010-06-15" version="1">MLHELFLLLSGHPSPILTPSGLLPSFPLVSPSERALLSSLSSLGHLHISLRNACSTITTTHPSTIARAVASGISTSHLHAFRARILSVEQQVLSRSAGMVGGYDIVSLAKIMSEFEGWERILSYLHSLASAMGEMSGGQLINRLRTDIHTGFPTLEAVSQHLLKVAETAWLREVSTWVLYGRLPSSEDFFITESESVVLNPVNEDETGVLKEYTIAKSRLPSFVTPDTASSILFIGRALSVIRLRGGANTGIASDTISPEMTLLPVHLSHLQSLQSPLSPQKLLTAVSEIRLSLSKHTLQKLLPASKIIDVVHVLREFFLLGRGEFAGAIVEQASEQVRNRWRRPGASGGMGASGVKSSSSSAVVKEGEVSSILTRTWGVLSSLQGEETTDERLDTARDLLYLSLVKPPPAASVASTPVHPKAKLGESFKDLLVGVPVTLNFHLSWPLELFLQPADLETYDAIFAYLISVRHTLIRLQTLWRGRRYPPAPLAFSKSENRAVAKARREVIKKREGIERAVWATAGLSVFFLETLVGYWQGEVVGGAFGELMEILGQGLEEAPEEEQQRQQEDEDGDLWMADGEDQDVRNVEEKTKEQQDPESLMREHHLYLSRLKRGLFLADHQFAPLLKKFLTSSEALASRIERMGRRNRLTDLNITPDTSTNDREVAECMASCRLVRNLLGMLIERLQRIDEERDIGGGELLSSGRKGGGGVGKIDRLLMRLDLANLRLGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLP6</accession>
    <name>D5GLP6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00010303001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430350">
        <property type="protein sequence ID" value="CAZ85439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841248.1">
        <property type="nucleotide sequence ID" value="XM_002841202.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLP6"/>
    <dbReference type="GeneID" id="9182742"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010303001"/>
    <dbReference type="eggNOG" id="ENOG502RYAK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014763_0_0_1"/>
    <dbReference type="InParanoid" id="D5GLP6"/>
    <dbReference type="OMA" id="WHYGVAH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="274"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="305"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="427"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="459"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="492"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="550"/>
            <end position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="640" mass="72166" checksum="1AF5EB03F1E25933" modified="2010-06-15" version="1">MAEGRGRLEGQCSRVSLHGNAHQGPQHPNTDTPRSLLRLVPSGKPLLLALFLLLLLSTPVPVAAKTHFAQCGAKIKELAKNGSLPSEYIYTGPVKGPLGQARNSKNPAFLVTFAACEEYCGVTPEYYEWEKASDTITTWVLPLVGLILQAPFESNNFKQTMFVVFRWVGSPIVSMMCIFWNMKVTRKCAIMVDMSVDKDQYPPPPSSFAELRDSFYLLSVVNQYELNIYTQLQERHIETILRVGLFDRRREIQRMRARVAASIRRERRRGTVQVLASLAWFLVAMGISINKAFGDLGVNSTAHNLALGLLMSWLPVLIAATIVDRNPMDARYVRAKLNNYLREIEAVERTPSIFPGVEDPDIFGEFAGQGRVRWHYGVAHSFLRTLEAMHGMGRGWSTIYENTDELSTTGAYSGLVGFDLSEVWQMVAAFLMVCSCSLGAFFVSYYTPTVGLGCRSGGYMIFGLNATGCLILEMAAWAFLHPGTGRRMAQWVLRLCEFTNFCWLVYIIVAQTFGIYNSCRCKSSMWGGAGGYVDFEGVPYYKSYHIENSWLLGTLTTCIFLGIGLAYVVEQWCTQSFLWTQNWESAMNGLWCVRKWKWATTGPRWVFAWLAEYVVEAECRVRTFLGREGDVEDCRPLWNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLS0</accession>
    <name>D5GLS0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 12">Long-chain-alcohol oxidase</fullName>
            <ecNumber evidence="5 12">1.1.3.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">GSTUM_00010376001</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Long-chain fatty alcohol oxidase involved in the omega-oxidation pathway of lipid degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>a long-chain primary fatty alcohol + O2 = a long-chain fatty aldehyde + H2O2</text>
            <dbReference type="Rhea" id="RHEA:22756"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:17176"/>
            <dbReference type="ChEBI" id="CHEBI:77396"/>
            <dbReference type="EC" id="1.1.3.20"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the GMC oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.3.20" evidence="5 12"/>
    <dbReference type="EMBL" id="FN430351">
        <property type="protein sequence ID" value="CAZ85487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841296.1">
        <property type="nucleotide sequence ID" value="XM_002841250.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLS0"/>
    <dbReference type="STRING" id="656061.D5GLS0"/>
    <dbReference type="GeneID" id="9186471"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010376001"/>
    <dbReference type="eggNOG" id="ENOG502QSD8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008878_3_1_1"/>
    <dbReference type="InParanoid" id="D5GLS0"/>
    <dbReference type="OMA" id="YGVIYET"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046577">
        <property type="term" value="F:long-chain-alcohol oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000172">
        <property type="entry name" value="GMC_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007867">
        <property type="entry name" value="GMC_OxRtase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012400">
        <property type="entry name" value="Long_Oxdase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46056">
        <property type="entry name" value="LONG-CHAIN-ALCOHOL OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46056:SF12">
        <property type="entry name" value="LONG-CHAIN-ALCOHOL OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05199">
        <property type="entry name" value="GMC_oxred_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00732">
        <property type="entry name" value="GMC_oxred_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028937">
        <property type="entry name" value="Lg_Ch_AO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="FAD-binding" evidence="16">
        <location>
            <begin position="190"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="15">
        <location>
            <begin position="239"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase C-terminal" evidence="17">
        <location>
            <begin position="549"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="643"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="191"/>
            <end position="206"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR028937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR028937-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR028937-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF05199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAZ85487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="718" mass="77807" checksum="EA176BE41AD2B7F2" modified="2010-06-15" version="1">MPNQWITLYALTSAIIRPFTEDEITAYMQSVATDISKEHVRSFLLDEASYTNARFRSALEYTLEQKLPKSSVQAICFVLNLLDSVPGCLLLTFSLTPFHRQPVRVREDLIVSWLDQSSRLLFPPIRGFAKAITALTMMVWTRNADTDMYYGAVGYPEKTGAGVKALDGEPGDFYKFEFLDFSTSEKKVLECDVVIVGSGCGGAVAAARLAGEGLEVVVVDRGEWVSPDQFPLRETEALNKLFSNGLASTTADGVIALISGETFGGGGVVNWAASLQTQGFVREEWATKHKLPFFLSNDYQDCLDKVCERMGVTTEGIKHNHRNQAMIEGCRKLGYSTVAVPQNTGATLPGDKEGIVREHADGYCGNGCGCGKKGDGRKMGTTHTWLPDAARAGARFIQSYYTDRVLFSTDAKTKAMGVTGTWGPQKVPLKIMAKKVIVSAGSLNSPCLLLRSGLTNPNIGKHLHLHPGVTIQAFFPERTDPWVGSPLTTACTQFENLDGSYHGGKLETAAMGPLMAASPLPWLSSASLKRTLLNYRHSGLFFTIQRDRDSGMVTLNTCGEPVVKYTISEADREMIQTSLLGLAKILYVQGAEEILPAEEEGPYGHATHQFNDPGIMDRRFQRWLKKFAGHGMGGEKTVYGSAHQMGSCRMGNDGGVGSVVDPKGRVWGTEGLLVADASVLPSACGVNPMITVMAISEWIAGGVVTELKPQRGGGGVKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLX8</accession>
    <name>D5GLX8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Very long-chain fatty acid transport protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Very-long-chain acyl-CoA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">GSTUM_00010466001</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24 25">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Acyl-CoA synthetase required for both the import of long chain fatty acids (LCFAs) (C14-C18) and the activation very long chain fatty acids (VLCFAs) (C20-C26) by esterification of the fatty acids into metabolically active CoA-thioesters for subsequent degradation or incorporation into phospholipids. The transport and fatty acyl-CoA synthetase activities are genetically separable and are thus independent activities. Esterifies VLCFAs in the peroxisome matrix. The VLCFAs are actively transported into peroxisomes by a PXA1-PXA2 heterodimeric transporter in the peroxisomal membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a very long-chain fatty acid + ATP + CoA = a very long-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:54536"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58950"/>
            <dbReference type="ChEBI" id="CHEBI:138261"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Lipid droplet</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Peroxisome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430351">
        <property type="protein sequence ID" value="CAZ85545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841354.1">
        <property type="nucleotide sequence ID" value="XM_002841308.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLX8"/>
    <dbReference type="FunCoup" id="D5GLX8">
        <property type="interactions" value="119"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GLX8"/>
    <dbReference type="GeneID" id="9187325"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010466001"/>
    <dbReference type="eggNOG" id="KOG1179">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_46_3_1"/>
    <dbReference type="InParanoid" id="D5GLX8"/>
    <dbReference type="OMA" id="VWRQFLD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005324">
        <property type="term" value="F:long-chain fatty acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044539">
        <property type="term" value="P:long-chain fatty acid import into cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000019">
        <property type="entry name" value="Long-chain fatty acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43107:SF15">
        <property type="entry name" value="FATTY ACID TRANSPORT PROTEIN 3, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43107">
        <property type="entry name" value="LONG-CHAIN FATTY ACID TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="13" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="14 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="21" id="KW-0732">Signal</keyword>
    <keyword evidence="9 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072428" description="Very long-chain fatty acid transport protein" evidence="21">
        <location>
            <begin position="21"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="273"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="22">
        <location>
            <begin position="66"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="23">
        <location>
            <begin position="511"/>
            <end position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00051585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00060276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00068795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00078285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="CAZ85545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="640" mass="72019" checksum="D29CA337365EFC32" modified="2010-06-15" version="1">MAPPLALLAAALPLLSYLDAKHQLTYDYAVISSFLYSRIRCLLLERRGRLNPFYGLEKRALSPTDCERLFLIYQGREYSYKEAYELALKYAAWLRERYNVQRNEIIAIDFMNKPAMIWIWLGLWALGAKPALLNYNLEGDRLVHCVKISTARLMFVDVEVKGVLEGSEGQETRRRLETEGANREVVIFDEAAERVVETWKGFRPGDEERIGAKLSEMAMLVYTRQISTSSFSGTTGMPKAAIVSFQKTHYGSGFVSAWVELKKSDRFYTCMPLYHASAALLGFVGAMMSGFTLVLGHRFSTKTFWPEVRSSRATILQYVGETCRYLLAAPPSPDDKDHNVKIAFGNGLRPDVWVRFKERFGIPAIAEFYASTEGTSGSWNLQRGEWGVGAVGRSGSLVSLLLGSGVKIAKMDAGNEELFRDENGFCVQCGYDEAGEVLWKLDPNDIKKTFQGYFRNPKASDEKVVRDAFKKGDAYFRTGDLQKRTRDGLWYFIDRIGDTYRWKSENVSSSEVSEVFGLHPSIAEANVYGVALPNHDGRCGCAAIVISPPTSDDEVRRELSKWVTKLPRFARPVFVRVVKAPGGLEKTGNYKFVKGTLRDEGADPEKVGASGDVLWWLKDGEYVPFGRGDWEGIVWGRVKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMC8</accession>
    <name>D5GMC8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed RNA polymerases I, II, and III subunit RPABC5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00010653001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the archaeal Rpo10/eukaryotic RPB10 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430354">
        <property type="protein sequence ID" value="CAZ85671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841480.1">
        <property type="nucleotide sequence ID" value="XM_002841434.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMC8"/>
    <dbReference type="FunCoup" id="D5GMC8">
        <property type="interactions" value="462"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GMC8"/>
    <dbReference type="GeneID" id="9183241"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010653001"/>
    <dbReference type="eggNOG" id="KOG3497">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_143122_2_1_1"/>
    <dbReference type="InParanoid" id="D5GMC8"/>
    <dbReference type="OMA" id="YCCRRMF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006360">
        <property type="term" value="P:transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000024">
        <property type="entry name" value="DNA-directed RNA polymerases I, II, and III subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00250">
        <property type="entry name" value="RNApol_arch_Rpo10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023580">
        <property type="entry name" value="RNA_pol_su_RPB10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020789">
        <property type="entry name" value="RNA_pol_suN_Zn-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000268">
        <property type="entry name" value="RPABC5/Rpb10"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003089">
        <property type="entry name" value="PRK04016.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23431:SF3">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23431">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC5 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01194">
        <property type="entry name" value="RNA_pol_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005653">
        <property type="entry name" value="RNA_pol_N/8_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46924">
        <property type="entry name" value="RNA polymerase subunit RPB10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01112">
        <property type="entry name" value="RNA_POL_N_8KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ85671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="73" mass="8534" checksum="C163266D122A3A8A" modified="2010-06-15" version="1">MIIPIRCFSCGKVVGDLWERYLKLLEDGVAEGDAMDQLGCKRYCCRRMLMTHVDLIEKLLRYNPQERTRIGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMU3</accession>
    <name>D5GMU3_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 10">Inositol-pentakisphosphate 2-kinase</fullName>
            <ecNumber evidence="4 10">2.7.1.158</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00010933001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Has kinase activity and phosphorylates inositol-1,3,4,5,6-pentakisphosphate (Ins(1,3,4,5,6)P5) to produce 1,2,3,4,5,6-hexakisphosphate (InsP6), also known as phytate.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Phosphorylates Ins(1,3,4,5,6)P5 at position 2 to form Ins(1,2,3,4,5,6)P6 (InsP6 or phytate).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>1D-myo-inositol 1,3,4,5,6-pentakisphosphate + ATP = 1D-myo-inositol hexakisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20313"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57733"/>
            <dbReference type="ChEBI" id="CHEBI:58130"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.158"/>
        </reaction>
    </comment>
    <comment type="domain">
        <text evidence="10">The EXKPK motif is conserved in inositol-pentakisphosphate 2-kinases of both family 1 and 2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the IPK1 type 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.158" evidence="4 10"/>
    <dbReference type="EMBL" id="FN430359">
        <property type="protein sequence ID" value="CAZ85836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841645.1">
        <property type="nucleotide sequence ID" value="XM_002841599.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMU3"/>
    <dbReference type="STRING" id="656061.D5GMU3"/>
    <dbReference type="GeneID" id="9185172"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010933001"/>
    <dbReference type="eggNOG" id="ENOG502S7VH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_522761_0_0_1"/>
    <dbReference type="InParanoid" id="D5GMU3"/>
    <dbReference type="OMA" id="WGLIVED"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035299">
        <property type="term" value="F:inositol-1,3,4,5,6-pentakisphosphate 2-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032958">
        <property type="term" value="P:inositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.110">
        <property type="entry name" value="Inositol-pentakisphosphate 2-kinase, N-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009286">
        <property type="entry name" value="Ins_P5_2-kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043001">
        <property type="entry name" value="IP5_2-K_N_lobe"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14456">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14456:SF2">
        <property type="entry name" value="INOSITOL-PENTAKISPHOSPHATE 2-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06090">
        <property type="entry name" value="Ins_P5_2-kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="261"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ85836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="441" mass="49524" checksum="A93BE8481E73182B" modified="2010-06-15" version="1">MEHSLIPPPLEGYVLDDAEEWEYFGEGAANVLYRYIGADPFFGPYLLRLRKRLPGAPTTQQLYADLHSRWGPLLDNCLLKTEIVKLGINIIPDLNSMFAMGDGEEATDWSGRPIKPPKQRRLLDDTESWGLIVEDMSCGNYLYNEMETQLDSMQEGEEIDDGRDEGSDEWKDMAMIEFKPKWLAQSPNAPANWLLCRTCAVRLMQSAKRQTNDLLCVPGEEGHDLEEEALDFCPLDLSSGDPTRIEKATFAILNMENSVMIADPTPTSDDEGDSDTPTPPTLNQRLAKCLENSLVTLLTNFFRTSPIIPLLTALQSRFGSRGVFTSATLALMGEKDVLPSEDEIRRTCLATGEKYEPAVSDEDDIWTAMTLRDCSLFLKVWVRKRRGRGMEAKIDAKVGDLDVKGGEGGRAVYWRDVERRLRMGGWYSGKGGTRNCRTGQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMY1</accession>
    <name>D5GMY1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00011018001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430361">
        <property type="protein sequence ID" value="CAZ85894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841703.1">
        <property type="nucleotide sequence ID" value="XM_002841657.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMY1"/>
    <dbReference type="FunCoup" id="D5GMY1">
        <property type="interactions" value="611"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GMY1"/>
    <dbReference type="GeneID" id="9183462"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011018001"/>
    <dbReference type="eggNOG" id="KOG4391">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029375_2_0_1"/>
    <dbReference type="InParanoid" id="D5GMY1"/>
    <dbReference type="OMA" id="QYWTSED"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008474">
        <property type="term" value="F:palmitoyl-(protein) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277">
        <property type="entry name" value="ALPHA/BETA HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277:SF81">
        <property type="entry name" value="PROTEIN ABHD13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="97"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ85894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="305" mass="33974" checksum="E73A9622A997D0B7" modified="2010-06-15" version="1">MSLFGAISSYSSSAYSLAKGTIVASTGLAVLLSGLLYFKQTELIYPRSIPEGSRTKVPTPDEFGVENWEHVELKTPDGESLKCYFLRGQRRMDMGVTVLFMHGNAGNIGHRLPIARVFSEEMGANIFILSYRGYGLSSGRPCEKGLNVDAQVALEYLLKRSDTKNNKIVVYGQSLGGALSIQLVSRNQDKVHGLILENTFRSIRTLIPTVFPPARFLAKLCHQIWPSEATLPQIVDVPVLFLSGLKDELVPPSHMKTLFDICRAKKVWRELPDGNHNETVAQDGYFDFIHDFIQKIRNGHTSDLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5GN00</accession>
    <name>D5GN00_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00010993001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430361">
        <property type="protein sequence ID" value="CAZ85875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841684.1">
        <property type="nucleotide sequence ID" value="XM_002841638.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GN00"/>
    <dbReference type="FunCoup" id="D5GN00">
        <property type="interactions" value="1412"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GN00"/>
    <dbReference type="GeneID" id="9183443"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010993001"/>
    <dbReference type="eggNOG" id="KOG1351">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022916_0_0_1"/>
    <dbReference type="InParanoid" id="D5GN00"/>
    <dbReference type="OMA" id="KDHGNVS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18112">
        <property type="entry name" value="ATP-synt_V_A-type_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01135">
        <property type="entry name" value="V_A-ATPase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055190">
        <property type="entry name" value="ATP-synt_VA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022879">
        <property type="entry name" value="V-ATPase_su_B/beta"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003235">
        <property type="entry name" value="PRK04196.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43389">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43389:SF4">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22919">
        <property type="entry name" value="ATP-synt_VA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit nucleotide-binding" evidence="6">
        <location>
            <begin position="1"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="ATP synthase A/B type C-terminal" evidence="7">
        <location>
            <begin position="172"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="274"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="275"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF22919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ85875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="312" mass="35306" checksum="2E30D12399FB79FE" modified="2010-06-15" version="1">MGVNLETSRFFTRDFEENGSLERVTLFLNLANDPTIERIITPRLALTTAEYFAYQLEKHVLVILTDLSAYCDALREVSAAREEVPGRRGYPGYMYTDLSTIYERAGRVEGRNGSITQIPILTMPNDDITHPIPDLTGYITEGQIFVDRQLHNRGIYPPINVLPSLSRLMKSAIGEGMTREDHGDVSNQLYAKYAIGRDAAAMKAVVGEEALSAEDKLSLEFLEKFERSFIAQGAYESRTIFESLDLAWSLLRIYPKELLNRIPAKIINEYYQRSTSDRKGKGKAREGGKDTRDNSREDAPKQPPQEENLIET</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GN23</accession>
    <name>D5GN23_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00011056001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430362">
        <property type="protein sequence ID" value="CAZ85916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841725.1">
        <property type="nucleotide sequence ID" value="XM_002841679.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GN23"/>
    <dbReference type="GeneID" id="9185842"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011056001"/>
    <dbReference type="HOGENOM" id="CLU_2759655_0_0_1"/>
    <dbReference type="InParanoid" id="D5GN23"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="70" mass="7975" checksum="F0F38956FA92DFB0" modified="2010-06-15" version="1">MEIPILPYMVYQYTFKRLSGWMVKIAQNNQGGFFFWWLASRVDTAPAVLCDDDDDDDGDDDGDGGFGCRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>D5GN79</accession>
    <name>D5GN79_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00011141001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430362">
        <property type="protein sequence ID" value="CAZ85972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841781.1">
        <property type="nucleotide sequence ID" value="XM_002841735.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GN79"/>
    <dbReference type="STRING" id="656061.D5GN79"/>
    <dbReference type="GeneID" id="9185898"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011141001"/>
    <dbReference type="eggNOG" id="ENOG502RD9J">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_508246_0_0_1"/>
    <dbReference type="InParanoid" id="D5GN79"/>
    <dbReference type="OMA" id="DNEWISA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="282"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="282"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="356"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="407"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="427"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="486"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="536" mass="57618" checksum="185D13F155A0C843" modified="2010-06-15" version="1">MTPPTSALRAISDRPSTAGAILLTPPNIVIDDSKASEVIVPSLKRPATASPSLSSAPGSANNSPTKRSPSKKMHKKALSDSPLAATGSFSDNRDISSIGASDEVPAPSEEMPSESTYEKRKQKKKKSKKQIKNWAGTILGKGRGLRHSKRQLRALPRRSPTPPLPRPEHQLGDNEWISAAWNESYVLMPVDDSLGANSSSENIAMDTGVESPIIDLDAALGPFKTPTGPSAGFAAARRRRMHSAVGLKGNGYFHRRSESMPEMQLFSLSEHEGDGHMADVFEEDEEEDSEESSESEDDDEEAKMDGGEGLGIGIKVVDGDGANWQEDVVMEWGSDDLGRPTSRRGSGGDRDLGWKSESSLLSANTSLNPESSTTSINSINCPQDIEVGSSSPLTLSGRKGAPADIITPQSSSSTVTLPAHFQPPTYPQSLDSPSTFVTAASNPNTPLQLDFPGDEYPDSASSFGPYSDFLGEPGPEMRMSVDDIPSLTSSSSTMTIGGLYANMPSTPMNEPKEKEPSHKKEKSKRWSKVWTFWKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNT3</accession>
    <name>D5GNT3_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Polyadenylate-binding protein, cytoplasmic and nuclear</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Polyadenylate tail-binding protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">GSTUM_00011471001</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the polyadenylate-binding protein type-1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430368">
        <property type="protein sequence ID" value="CAZ86176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841985.1">
        <property type="nucleotide sequence ID" value="XM_002841939.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNT3"/>
    <dbReference type="STRING" id="656061.D5GNT3"/>
    <dbReference type="GeneID" id="9182811"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011471001"/>
    <dbReference type="eggNOG" id="KOG0123">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_22_1_1"/>
    <dbReference type="InParanoid" id="D5GNT3"/>
    <dbReference type="OMA" id="CTESTLY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12378">
        <property type="entry name" value="RRM1_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12379">
        <property type="entry name" value="RRM2_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12380">
        <property type="entry name" value="RRM3_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000003">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000355">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034364">
        <property type="entry name" value="PABP_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045305">
        <property type="entry name" value="RRM2_I_PABPs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="11 14" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="10" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RRM" evidence="16">
        <location>
            <begin position="53"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="16">
        <location>
            <begin position="141"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="16">
        <location>
            <begin position="234"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAZ86176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="374" mass="41448" checksum="668CE3862E9984E2" modified="2010-06-15" version="1">MSAEVSSSPAPQEQIEQPINGSSPAQDPTSDSDANNSSVAAPHSAPHNSHTSASLYVGELEPSVTEAMLFELFSTIGNVASIRVCRDAVTRRSLGYSYVNYNNTADGERALEELNYTLIKGRPCRIMWSQRDPALRKTGQGNVFIKNLDTAIDNKALHDTFAAFGNILSCKVAQDEFSNSRGYGFVHYETAEAANNAIKHVNGMLLNEKKVFVGHHIPKKDRQSKFDEMKANFTNVYVKNIDPEVTDEEFRTMFENYGPITSASLSRDQDGVSRGFGFVNFQEHQHAAKAVEELHESELKGQTLYPLELRKLASTRVSICTSRISMTRSTTSVSARSLPRMELSLPPRLCVMSLVRVRDLVLSASLARRKLARP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNU7</accession>
    <name>D5GNU7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00011517001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cycloisomerase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430370">
        <property type="protein sequence ID" value="CAZ86190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841999.1">
        <property type="nucleotide sequence ID" value="XM_002841953.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNU7"/>
    <dbReference type="STRING" id="656061.D5GNU7"/>
    <dbReference type="GeneID" id="9182845"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011517001"/>
    <dbReference type="eggNOG" id="ENOG502S0CU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038716_0_1_1"/>
    <dbReference type="InParanoid" id="D5GNU7"/>
    <dbReference type="OMA" id="CPRHFMF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017057">
        <property type="term" value="F:6-phosphogluconolactonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050282">
        <property type="entry name" value="Cycloisomerase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011048">
        <property type="entry name" value="Haem_d1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019405">
        <property type="entry name" value="Lactonase_7-beta_prop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30344:SF1">
        <property type="entry name" value="6-PHOSPHOGLUCONOLACTONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30344">
        <property type="entry name" value="6-PHOSPHOGLUCONOLACTONASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10282">
        <property type="entry name" value="Lactonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51004">
        <property type="entry name" value="C-terminal (heme d1) domain of cytochrome cd1-nitrite reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072856" evidence="2">
        <location>
            <begin position="20"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ86190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="386" mass="40828" checksum="BC3DB77DD40ADF71" modified="2010-06-15" version="1">MSLRSILKTILLMASTASAARTLFVGSAKGTIKTYEFDETANYQSSIKELASTSESSPAPTWQTIFGNLLYSVSQTEVTADGVVTAYQIGSDKKLIKKGSARGIAGPVSIAVCKGGKLVITASYGGGGVDTFAADGSGGLTHSQRFAYKLNKPGAVPDRQTEPHPHQAIMDPTGEYVIVPDLGADQVRVYNVNSSTTIKELECIKTPPGFGPRHAVFYPVGNSKATHLFVVGELANKIITFKISYDHGRLKAKKVATVSTFGAQIVPTGPSALSAAEIALSPNGKYLYVSNRFDKTQAGKTQDSIAIFSVASDAALTFMQLSASGGLEPRHFSITRDGNWVAVANVGNGNLGIFRRHPENGTLSSSPVISMPNDGASCALWYEPQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNW6</accession>
    <name>D5GNW6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00011546001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430370">
        <property type="protein sequence ID" value="CAZ86209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNW6"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011546001"/>
    <dbReference type="HOGENOM" id="CLU_1972081_0_0_1"/>
    <dbReference type="InParanoid" id="D5GNW6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ86209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="127" mass="14481" checksum="5E83F6062D1D92F4" modified="2010-06-15" version="1">MKQHPKHPSMPSSVYGSRTVPLGLSGSAQRCRELLRGRKSSSVPRGVMDHYEADGSGMFSAFKDYPYFAEPSRLSTGHKSSRTVNQRNKMPARKPQSRPLYLRLTLILTLQYFLPFHSLLEIIIRFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>D5GP37</accession>
    <name>D5GP37_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00011660001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430372">
        <property type="protein sequence ID" value="CAZ86280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842089.1">
        <property type="nucleotide sequence ID" value="XM_002842043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GP37"/>
    <dbReference type="STRING" id="656061.D5GP37"/>
    <dbReference type="GeneID" id="9186519"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011660001"/>
    <dbReference type="HOGENOM" id="CLU_1020111_0_0_1"/>
    <dbReference type="InParanoid" id="D5GP37"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ86280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="273" mass="28774" checksum="A27F56C24CF4F283" modified="2010-06-15" version="1">MTQNSVQGGSNASAGHANHGKKSSDTAWLVVSIAVTVPSALYLLYSSPAKKSDYHASGHHSSTSTLEDKGGALSGGETANSQMDHAAESVSLAAEDTREQANERLGEIGEKAGELYGGAKDRAETSIMSATDKTAYVKEEAGEVPEPTTEGSKNRTHEDDKGSQEVSGKPDQDGEKDLDDSRGREGADTPKEPVDERRKAEKTDGDSKSSETLSNLKKKQNPSKTPSQVDPPATGMKDAPSTDKRSGKQEGSDNGDAPHAVRTCPQPDVTCLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5GP63</accession>
    <name>D5GP63_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00011724001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430373">
        <property type="protein sequence ID" value="CAZ86328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842137.1">
        <property type="nucleotide sequence ID" value="XM_002842091.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GP63"/>
    <dbReference type="GeneID" id="9186568"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011724001"/>
    <dbReference type="HOGENOM" id="CLU_2639896_0_0_1"/>
    <dbReference type="InParanoid" id="D5GP63"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ86328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="77" mass="8769" checksum="50504533BA513CF7" modified="2010-06-15" version="1">MYQELGGYRKTHAHIRTHESVGCCRSVALVDSAFSLDIKLERVVLDCGNNPFLSPPLRRHHITRNVGAKAWWEHTVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPD2</accession>
    <name>D5GPD2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ATP-dependent rRNA helicase RRP3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">ATP-dependent rRNA helicase rrp3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">GSTUM_00011678001</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DEAD box helicase family. DDX47/RRP3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430373">
        <property type="protein sequence ID" value="CAZ86294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842103.1">
        <property type="nucleotide sequence ID" value="XM_002842057.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPD2"/>
    <dbReference type="FunCoup" id="D5GPD2">
        <property type="interactions" value="1080"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GPD2"/>
    <dbReference type="GeneID" id="9186534"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011678001"/>
    <dbReference type="eggNOG" id="KOG0330">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_1_1_1"/>
    <dbReference type="InParanoid" id="D5GPD2"/>
    <dbReference type="OMA" id="GIGIKCC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010467">
        <property type="term" value="P:gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17954">
        <property type="entry name" value="DEADc_DDX47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000681">
        <property type="entry name" value="probable ATP-dependent RNA helicase DDX47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044765">
        <property type="entry name" value="DDX47/Rrp3_DEADc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF24">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 13" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="17">
        <location>
            <begin position="82"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="15">
        <location>
            <begin position="113"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="16">
        <location>
            <begin position="307"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="472"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="12">
        <location>
            <begin position="82"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="472"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAZ86294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="513" mass="56092" checksum="2BA621C5801842ED" modified="2010-06-15" version="1">MGEGPGKKRRVSRGATVKERHVVERVAKITKDKNVKPESTGGQEGVGEEQEDGEGGISESETGTVAKLVPDAAGPSETAPLKSFKELGVMDSLCEACENLGYKNPTSIQVESIPVALEGKDLIGLAETGSGKTAAFAIPILQALWDNPTGLFACILAPTRELAFQISEQFEALGGGIGVRSAVIVGGMDMMTQSVALGKKPHILVATPGRLLDHLENTKGFSLRNLKYLVMDEADRLLDMDFGPILDKILKVIPQTRRTYLFSATMTSKVEKLQRASLSSPVRISVGSKYSTVSTLIQKFLFIPFKHKDTYLVYLMNEFAGQTTIIFCRTVQETSRLAILLRHLGFNAVPLNGQMSQSARLGALNKFKSGSRNILVATDVAARGLDMHVSPDALLYCVLNHDLAQDSKTYIHRVGRTARAGRSGRSISLVTQYEVELFLRIEAALGKKIPEFDVQEEDVLIFSERVGEAQREAAKQLREEREKKGGRREARSGGVGSKGKGKRRRDDMDQEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPV7</accession>
    <name>D5GPV7_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Putative transcription factor kapC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00012070001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Putative transcription factor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the bZIP family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430379">
        <property type="protein sequence ID" value="CAZ86559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842368.1">
        <property type="nucleotide sequence ID" value="XM_002842322.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPV7"/>
    <dbReference type="STRING" id="656061.D5GPV7"/>
    <dbReference type="GeneID" id="9182302"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012070001"/>
    <dbReference type="eggNOG" id="ENOG502SC5V">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_568821_0_0_1"/>
    <dbReference type="InParanoid" id="D5GPV7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050936">
        <property type="entry name" value="AP-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40621">
        <property type="entry name" value="TRANSCRIPTION FACTOR KAPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40621:SF11">
        <property type="entry name" value="TRANSCRIPTION FACTOR KAPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="10">
        <location>
            <begin position="271"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="162"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="335"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="449"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="185"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="205"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="272"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="345"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="450"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS00036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ86559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="480" mass="51803" checksum="5FA335C32374F885" modified="2010-06-15" version="1">MLSLNPFPPSYSQLTTSFFPFPSHQRTNERASERTNRSVIKHYYYFLFFLHFFLQAKAIFFQLEKVRTSKNKTKKKRISQIIILHPRSINYPPHPDRTGEHRNRHFSYPELSRTTKGKVSHFYKMQQSTLAPAPHPLQSPSAQEAANTAEQTLRAHLLPHIHSPQQQQQQQQQQVQQQVQQQQQNVTSPDNKPTDSASPDLNIDPAISGVGASGGVSSVPQSPGLTTMAIQGMSPGSQAAMEQHSGAEETQAEGSSAGRGRGGKRELSTSKRAAQNRAAQRAFRQRKEGYIKKLEEQVRDFNQMQESYKQIQAENYQLRDYIINLQSRLIEHQGEEAVPPPPLPLLGSSSSTTSAIPLPTTTTTPALPPQQQQQPPHPPLTDPSAPTANMGTVVELSSNSAANPPASNPVGAKRAHEDDAANAFLQGVAAAGNGFLQAQQVAAAAVITGTGTGAGSGGGGGGGGGGKRLKNGDESGVNGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPW7</accession>
    <name>D5GPW7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00012081001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430379">
        <property type="protein sequence ID" value="CAZ86569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842378.1">
        <property type="nucleotide sequence ID" value="XM_002842332.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPW7"/>
    <dbReference type="FunCoup" id="D5GPW7">
        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GPW7"/>
    <dbReference type="GeneID" id="9182312"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012081001"/>
    <dbReference type="eggNOG" id="KOG4137">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071116_2_1_1"/>
    <dbReference type="InParanoid" id="D5GPW7"/>
    <dbReference type="OMA" id="VIKPMAP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034080">
        <property type="term" value="P:CENP-A containing chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029525">
        <property type="entry name" value="INO80C/Ies6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013272">
        <property type="entry name" value="Vps72/YL1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31200">
        <property type="entry name" value="INO80 COMPLEX SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31200:SF1">
        <property type="entry name" value="INO80 COMPLEX SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08265">
        <property type="entry name" value="YL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00993">
        <property type="entry name" value="YL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Vps72/YL1 C-terminal" evidence="5">
        <location>
            <begin position="86"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ86569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="137" mass="15065" checksum="CD3E015503AC2899" modified="2010-06-15" version="1">MAPPAAPPQTNIVLDELDITPHPRPFKNPNWKPPHRRTKTLKQILSDATRTAATGEGGTTPATGPMQTTYANIESAPSLHPAHSKKWCDITGLPAKYTDPKTKLRYADKEVYTVIRMLPPGSAEKYLELRGANVVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPZ8</accession>
    <name>D5GPZ8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00012112001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430380">
        <property type="protein sequence ID" value="CAZ86591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842400.1">
        <property type="nucleotide sequence ID" value="XM_002842354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPZ8"/>
    <dbReference type="GeneID" id="9182334"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012112001"/>
    <dbReference type="HOGENOM" id="CLU_2544259_0_0_1"/>
    <dbReference type="InParanoid" id="D5GPZ8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ86591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="83" mass="9609" checksum="A8A5312F82D24B8F" modified="2010-06-15" version="1">MHGGAKLVKRVVEYSTSTVRIMREAEEFLENPEHDLNADTLTREDSKQPLPWLKPSNSKLRSLSCSPSSLILYTRIRRFSFEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5G3Y4</accession>
    <name>D5G3Y4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glutamine-dependent NAD(+) synthetase</fullName>
            <ecNumber evidence="8">6.3.5.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">NAD(+) synthase [glutamine-hydrolyzing]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00003858001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>deamido-NAD(+) + L-glutamine + ATP + H2O = L-glutamate + AMP + diphosphate + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24384"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">In the C-terminal section; belongs to the NAD synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.1" evidence="8"/>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835106.1">
        <property type="nucleotide sequence ID" value="XM_002835060.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G3Y4"/>
    <dbReference type="FunCoup" id="D5G3Y4">
        <property type="interactions" value="707"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G3Y4"/>
    <dbReference type="GeneID" id="9187165"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003858001"/>
    <dbReference type="eggNOG" id="KOG2303">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011884_2_0_1"/>
    <dbReference type="InParanoid" id="D5G3Y4"/>
    <dbReference type="OMA" id="TSQEVCN"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00334"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003952">
        <property type="term" value="F:NAD+ synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034354">
        <property type="term" value="P:'de novo' NAD+ biosynthetic process from L-tryptophan"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07570">
        <property type="entry name" value="GAT_Gln-NAD-synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00553">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000036">
        <property type="entry name" value="Glutamine-dependent NAD(+) synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.110.10:FF:000003">
        <property type="entry name" value="Glutamine-dependent NAD(+) synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02090">
        <property type="entry name" value="NadE_glutamine_dep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014445">
        <property type="entry name" value="Gln-dep_NAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003694">
        <property type="entry name" value="NAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00552">
        <property type="entry name" value="nadE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23090:SF9">
        <property type="entry name" value="GLUTAMINE-DEPENDENT NAD(+) SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23090">
        <property type="entry name" value="NH 3 /GLUTAMINE-DEPENDENT NAD + SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006630">
        <property type="entry name" value="NADS_GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 8" id="KW-0520">NAD</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="9">
        <location>
            <begin position="5"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00052340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR006630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ79227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="705" mass="79411" checksum="0BCE6C08265D49AE" modified="2010-06-15" version="1">MGHLAVLATCSLNQWALDFEGNAERIIESIRIAKEAGASLRVGPELEITGYGCLDHFLEGDTYLHSWEILAKIIAHEDCQDILLDIGMPIGHKNIRYNCRVIAHNKRILLIRPKIYLANDGNYREMRYFTGWGRTKYVEEYYLPRIIMTITGQRKVPFGDAAISTLDTCIAPETCEEMFTPNSPHIGMSLDGIEIMTNSSGSHHELRKLQTRIELMTEATKKCGGIYLYANQQGCDGDRLYYDGSAMIGINGKIVAQGTQFSLQDVEVITATVDLEEVRSFRFAPSRGVQAVQNTPYQRIEVDISISAPSDRAEISLKPTPTIDIRYHSPEEEIALGPACWLWDYLRRCGGVSGYFLPLSGGIDSCATATIVHSMCRLVHEACENGEEQVIKDARRVCGEPEESTWVPRTPQELAGRIFHTCFMGTKNSSADTRARAKELADAIGAYHIDLDMDFLVKAVTDLFSLVTGKRPQFRVHGGTKTENLALQNIQARLRMVLAYLFAQLLPWCRGKQGGLLVLGSANVDECLRGYMTKYDCSSADINPIGGISKTDLKRFISYATAKFNLPILHSFLTAIPTAELEPITSTYVQSDEADMGFTYDELSTFGQLRKNHKLGPWSAFNRLLHEWGDRMSPRAIATKTRNFFYYYAINRHKMTVLTPAYHAEQYSPDDNRFDLRPFLYPPFSWPFRKIEETVAAINAREKMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4M7</accession>
    <name>D5G4M7_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phenylalanine--tRNA ligase beta subunit</fullName>
            <ecNumber evidence="5">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Phenylalanyl-tRNA synthetase beta subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">GSTUM_00000015001</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>tRNA(Phe) + L-phenylalanine + ATP = L-phenylalanyl-tRNA(Phe) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="5"/>
    <dbReference type="EMBL" id="FN429989">
        <property type="protein sequence ID" value="CAZ79470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835313.1">
        <property type="nucleotide sequence ID" value="XM_002835267.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4M7"/>
    <dbReference type="FunCoup" id="D5G4M7">
        <property type="interactions" value="1061"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G4M7"/>
    <dbReference type="GeneID" id="9188407"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000015001"/>
    <dbReference type="eggNOG" id="KOG2472">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020279_2_0_1"/>
    <dbReference type="InParanoid" id="D5G4M7"/>
    <dbReference type="OMA" id="FPGRCAN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009328">
        <property type="term" value="C:phenylalanine-tRNA ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00769">
        <property type="entry name" value="PheRS_beta_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.40.10:FF:000002">
        <property type="entry name" value="phenylalanine--tRNA ligase beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.56.10:FF:000006">
        <property type="entry name" value="Phenylalanyl-tRNA synthetase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.56.10:FF:000004">
        <property type="entry name" value="Phenylalanyl-tRNA synthetase, beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000052">
        <property type="entry name" value="Phenylalanyl-tRNA synthetase, beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.40.10">
        <property type="entry name" value="Phenylalanyl-trna Synthetase, Chain B, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005146">
        <property type="entry name" value="B3/B4_tRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045060">
        <property type="entry name" value="Phe-tRNA-ligase_IIc_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004531">
        <property type="entry name" value="Phe-tRNA-synth_IIc_bsu_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020825">
        <property type="entry name" value="Phe-tRNA_synthase-like_B3/B4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041616">
        <property type="entry name" value="PheRS_beta_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040659">
        <property type="entry name" value="PhetRS_B1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005147">
        <property type="entry name" value="tRNA_synthase_B5-dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00471">
        <property type="entry name" value="pheT_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947:SF0">
        <property type="entry name" value="PHENYLALANINE--TRNA LIGASE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947">
        <property type="entry name" value="PHENYLALANYL-TRNA SYNTHETASE BETA CHAIN AND LEUCINE-RICH REPEAT-CONTAINING PROTEIN 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03483">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03484">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18262">
        <property type="entry name" value="PhetRS_B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17759">
        <property type="entry name" value="tRNA_synthFbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00873">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00874">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56037">
        <property type="entry name" value="PheT/TilS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51483">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0436">Ligase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B5" evidence="17">
        <location>
            <begin position="294"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00033189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAZ79470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="592" mass="66689" checksum="66C34E832763097D" modified="2010-06-15" version="1">MPTVSVNKYDLYEALGQQYTTEEFDDLCFEFGIELDEDTENDPDRPKDQPPQLKIEIPANRYDMLCHEGISLMLNIFREKASIPNYRVVSPPNGEVHQIIVHEETTKIRPYVSAAVLRGIKFTQGRYDSFIALQDKLHQNLCRQRTLVSMGTHDLDTVKGPFTYEALPPEEIVFAPLNKEEVMNGKQLMEFYENDKHLGRYLYIIRDSPAYPVILDANRRVCSLPPIINSNLSKITLDTRNIFIEVTATDRTKAEIVTNILVAMFSQYCDVSFTVEPVKIISPHNNESRQVPNVAPRSTTAEVSYINSCCGLQLSPSEICTMLDKMAYTAKPSSSDPNLLDIDVPVTRADVLHQCDIMEDVAIGYGFNNLPKTFPGKSGTIAAPLPINKLSDIVRLEAAMCGWSEVLPLILCSHDENFAWLNRKDDGHTAVRLANPKTAEYQIVRTSLLPGVLKTIRENRKHSLPIKVFEVSDVAFKDEKLERKSRNERHFCAAVCGKTSGFEAVHGLLDRVMAMLGTKVLDTGEMEQEGYWIEAADEPTHFPGRAASILVRHKNGKRQSIGTFGILHPSVLEKFEIPYPVSTLELNLEVFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4W7</accession>
    <name>D5G4W7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00000124001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429992">
        <property type="protein sequence ID" value="CAZ79560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835403.1">
        <property type="nucleotide sequence ID" value="XM_002835357.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4W7"/>
    <dbReference type="GeneID" id="9183042"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000124001"/>
    <dbReference type="HOGENOM" id="CLU_3385067_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4W7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ79560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="33" mass="3719" checksum="4C1BC562481F0693" modified="2010-06-15" version="1">MHFFLLKSTHTCSFAGSATNWSGCFLLIASHFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4X6</accession>
    <name>D5G4X6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00000139001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429993">
        <property type="protein sequence ID" value="CAZ79568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835411.1">
        <property type="nucleotide sequence ID" value="XM_002835365.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4X6"/>
    <dbReference type="GeneID" id="9183050"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000139001"/>
    <dbReference type="eggNOG" id="ENOG502RGPE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1541226_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4X6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="174" mass="18734" checksum="F999E0C448316F82" modified="2010-06-15" version="1">MSDNEDSKQFAKERPISTIARSFSAQLNDAFLIDDTLDQLSSTVEQKKLSVTFHSKELEELEARIRRAEALLEEKKKRFSEQLPGEQPSDLSAPSTRPARQSMPDHDQPESSQAAAAPPNRPAPAPPVANGGSAKGEFVVIEKSASMPGESGNGPPAAVFGEQYSARPPPPVPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>D5G504</accession>
    <name>D5G504_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00000222001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S33 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429994">
        <property type="protein sequence ID" value="CAZ79597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835440.1">
        <property type="nucleotide sequence ID" value="XM_002835394.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G504"/>
    <dbReference type="STRING" id="656061.D5G504"/>
    <dbReference type="GeneID" id="9186294"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000222001"/>
    <dbReference type="eggNOG" id="ENOG502RY03">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013364_5_2_1"/>
    <dbReference type="InParanoid" id="D5G504"/>
    <dbReference type="OMA" id="WAEIRMS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013595">
        <property type="entry name" value="Pept_S33_TAP-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051601">
        <property type="entry name" value="Serine_prot/Carboxylest_S33"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43248">
        <property type="entry name" value="2-SUCCINYL-6-HYDROXY-2,4-CYCLOHEXADIENE-1-CARBOXYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43248:SF25">
        <property type="entry name" value="AB HYDROLASE-1 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08386">
        <property type="entry name" value="Abhydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="132"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S33 tripeptidyl aminopeptidase-like C-terminal" evidence="4">
        <location>
            <begin position="465"/>
            <end position="568"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ79597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="618" mass="66687" checksum="6CFF5E4CBF6ADEA2" modified="2010-06-15" version="1">MSYNPAEKFGAPSNGMDSEGKAYVHPGKPRSWGLSKSSKTFLATFMMLLLLYACRVRHSEPLFFVRRPVKGDYGWGDIIPTEDINLYPCFNGHKCARLAVPLDYTNPSPDHTAAIAITILEATDTSRPYGGPVLINPGGPGGSGVYMLLAFGELFQQVIGRHYSIVGFDPRGVNNTTPTFSCFEDSLRRKLFIEKDDGKVLTGGSEVGETYARFKALSESCGTVAGEEKGGYVNTPSVARDMLEISERLWGLSGMSGRGLQYWGLSYGSVLGSTFAAMFPERVERVVVDGICDVEDYYSAAWKANLQDTDKVLASFSTYCSQAGPLRCAFHTGSTPEDITVRLHNLLQKLSAEPLPIPSSQSTTSTPEIITESDVRNLLFASLYAPLGWFPIVAQILAALETGDGRGYLEVARAGFECDCPGGLRTPNTGGGEVALAIACSDGTPVPDDRAAFDEYLRELTSQTKAFGGVWAKLRLGCTNWKFRPKGLAFPGPYSGNTSTPLLIVGNSKDPVTPLRNAHVMSKNFPGSTVLEQNSEGHCSISAPSLCTAQHIRRYFETGEVPATGTVCEADMKPFIGAGDIIKTMSAEDAELLGALEKLAMELMPLFRRDFGPAPNAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>D5G558</accession>
    <name>D5G558_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00000266001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429996">
        <property type="protein sequence ID" value="CAZ79643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835486.1">
        <property type="nucleotide sequence ID" value="XM_002835440.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G558"/>
    <dbReference type="STRING" id="656061.D5G558"/>
    <dbReference type="GeneID" id="9186341"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000266001"/>
    <dbReference type="eggNOG" id="ENOG502QRMK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032013_0_1_1"/>
    <dbReference type="InParanoid" id="D5G558"/>
    <dbReference type="OMA" id="ATWNKRE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.580">
        <property type="entry name" value="Acetamidase/Formamidase-like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004304">
        <property type="entry name" value="FmdA_AmdA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054833">
        <property type="entry name" value="FormamaseFmdA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF045496">
        <property type="entry name" value="FormamaseFmdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31891">
        <property type="entry name" value="FORMAMIDASE C869.04-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31891:SF1">
        <property type="entry name" value="FORMAMIDASE C869.04-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03069">
        <property type="entry name" value="FmdA_AmdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141130">
        <property type="entry name" value="Acetamidase/Formamidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ79643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="413" mass="45298" checksum="A6749B29569611D1" modified="2010-06-15" version="1">MVAGPIRTALKVDFGKSAQEQRHFHNRWHPDIPTVGKIVPGEVVKVECVDCTGGRIQNNDCADDARDCDLTQPHYLSGPFEIEGADPGDVLVVEIQDVQPFEDRPWGFTAVLDRRNGGGFLDDHYPRSAKAIWDFEGIFCSSRHIPHVRFPGLIHPGIMGCAPSAEVLAEWNRRESELVSVNTLDRVVALCPQPEGVHAGSAEEDLRAKVGREGARTIPGRPEHGGNCDIKNLSRGSKTYLPVHVPGAKFSVGDLHFSQGDGEISFCGAIEMAGIITLKFELMKGGIRELDMKSPIFIPGPVEPHFGPGRMLTFEGFSVDEAGKQHYMDVTVAYRQTCLRVIEYLKRFGYSGEQIYLLLSCAPVQGHVAGIVDIPNACTTMSVPMDIFDFDISPGAPIPRLDMGSCAYTSDRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>D5G565</accession>
    <name>D5G565_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Flap endonuclease 1</fullName>
            <shortName evidence="15">FEN-1</shortName>
            <ecNumber evidence="15">3.1.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Flap structure-specific endonuclease 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">GSTUM_00000275001</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="15">Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Nucleus</location>
            <location evidence="15">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Nucleus</location>
            <location evidence="15">Nucleoplasm</location>
        </subcellularLocation>
        <text evidence="15">Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14 15">Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="15"/>
    <dbReference type="EMBL" id="FN429996">
        <property type="protein sequence ID" value="CAZ79650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835493.1">
        <property type="nucleotide sequence ID" value="XM_002835447.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G565"/>
    <dbReference type="FunCoup" id="D5G565">
        <property type="interactions" value="995"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G565"/>
    <dbReference type="GeneID" id="9186348"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000275001"/>
    <dbReference type="eggNOG" id="KOG2519">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032444_1_1_1"/>
    <dbReference type="InParanoid" id="D5G565"/>
    <dbReference type="OMA" id="MGIPWVQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008409">
        <property type="term" value="F:5'-3' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
        <property type="term" value="F:5'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043137">
        <property type="term" value="P:DNA replication, removal of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09907">
        <property type="entry name" value="H3TH_FEN1-Euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09867">
        <property type="entry name" value="PIN_FEN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000009">
        <property type="entry name" value="Flap endonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000003">
        <property type="entry name" value="Flap endonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00614">
        <property type="entry name" value="Fen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023426">
        <property type="entry name" value="Flap_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006086">
        <property type="entry name" value="XPG-I_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006084">
        <property type="entry name" value="XPG/Rad2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019974">
        <property type="entry name" value="XPG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006085">
        <property type="entry name" value="XPG_DNA_repair_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081:SF9">
        <property type="entry name" value="FLAP ENDONUCLEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081">
        <property type="entry name" value="FLAP ENDONUCLEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00867">
        <property type="entry name" value="XPG_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00752">
        <property type="entry name" value="XPG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00853">
        <property type="entry name" value="XPGRADSUPER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00484">
        <property type="entry name" value="XPGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00485">
        <property type="entry name" value="XPGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00841">
        <property type="entry name" value="XPG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00842">
        <property type="entry name" value="XPG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 15" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12 15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 15" id="KW-0235">DNA replication</keyword>
    <keyword evidence="6 15" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="9 15" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="8 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 15" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 15" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XPG N-terminal" evidence="18">
        <location>
            <begin position="99"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="XPG-I" evidence="17">
        <location>
            <begin position="241"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="449"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="449"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAZ79650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="481" mass="54205" checksum="C67C2CF2948FF123" modified="2010-06-15" version="1">MKTGITLVGLIDCLRKYTCKCFGIIFFLLSPQPRLNKARLRFSSASPSSPPSNLTFYTRFRQHLGLQQLSLLFTRWVLKVTTLSEQILHLRSLANTRYPGLFHLLQENTQDSIKDGEIKQHFGRKIAIDASMSIYSFLIAVRSEGQQLTSETGETTSHLMGLFYRTLRMVDNGIKPLYVFDGRPPTLKSGELAKRSARKAEATAAHAEAKEVGTAEEVEKFSRRTVRVTKEINDECKRLLTCMGIPYVEAPCEAEAQCAALAKAGKVYAAASDDMDTLCFETPILLKKLMLSEMKKEPVQEIYLDRAMQELGFTRDQFIDLCILLGCDYCDTIPKVGPTTALKLIREHKSIENVLSHLGPKYAVPKKWPYQDARELFKNPLITDPDECEFKWEAPDIDKLVAFLVGEKGFNEDRVRAGAVKLSKNLKGNTQARVNDFFKPVPKTAEELAGLKRKSEEKAAERKKKVKAEKAEKGKGKKGKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5M2</accession>
    <name>D5G5M2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00001485001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430000">
        <property type="protein sequence ID" value="CAZ79815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835658.1">
        <property type="nucleotide sequence ID" value="XM_002835612.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5M2"/>
    <dbReference type="GeneID" id="9183801"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001485001"/>
    <dbReference type="HOGENOM" id="CLU_2335165_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5M2"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ79815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="98" mass="10307" checksum="5F94D97C8BDE294F" modified="2010-06-15" version="1">MQTSSVPRNHLVKIGVSQPGCTKPAHLAHPGHDLLAPKGYCGDWLNASSGLLVGHPRSFGTFTASSWTRSSLPYPILLSAVGARFGGGHDICIGMKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5M8</accession>
    <name>D5G5M8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00001491001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430000">
        <property type="protein sequence ID" value="CAZ79821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835664.1">
        <property type="nucleotide sequence ID" value="XM_002835618.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5M8"/>
    <dbReference type="STRING" id="656061.D5G5M8"/>
    <dbReference type="GeneID" id="9187117"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001491001"/>
    <dbReference type="eggNOG" id="ENOG502RFD2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_507335_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5M8"/>
    <dbReference type="OMA" id="CRQQDWV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42069">
        <property type="entry name" value="HYPHAL ANASTAMOSIS-8 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42069:SF1">
        <property type="entry name" value="MARVEL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="214"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="260"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="296"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="361"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="462"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="485"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="528"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ79821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="537" mass="58479" checksum="0316EE44A8F41E29" modified="2010-06-15" version="1">MSSSPVSQNQPPPYSPVDGNGFSQHREQHQQTQQSSSEPEDFYRPGSQPRVNTVNTDPASDMDHISPVSPSSPADSQAPIMASTREQQLQDAVVGTAQEYPPPPLPVAVLPPPTVQEYSEHLNTSQTVYAPVSAYSTTSGVVNGEIGAVPGPTPRTPRMVMADGGYAVPMTPLTPALKTPTFKEEAEVSKYDEKVRKFDKKDLAVKLKVRLAKIFLRGINCACSLVVLALIASTFAIFNATKALPPRNNLPPWAVNTPQWPQITVLCIACVSLVISLYIMYSYWRGGHNRAEKVAVYWTVFAVGTFIFTIVIWAIAAGIMQGSRNSSEGKDLWGWACKDNNRRKLFEEEVNYRLVCRQQDWVLVCAIIEISVECVSIAVYAFAFYRLTSKRRLRKSMDIRDKARSDLWLAKLHEQQAEEAAAATNDPETNANTAYNKLTSATSPQELEEGRAVPVLMRPPPGHYATVPHKAPEVAGGDDARGINPSVTVTPPGSSQGPQELGVEPPLRSPALAIGAVPPTPRSVSFQAPPPGSRGSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5G648</accession>
    <name>D5G648_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00001768001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430007">
        <property type="protein sequence ID" value="CAZ79991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835834.1">
        <property type="nucleotide sequence ID" value="XM_002835788.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G648"/>
    <dbReference type="GeneID" id="9187670"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001768001"/>
    <dbReference type="HOGENOM" id="CLU_2795780_0_0_1"/>
    <dbReference type="InParanoid" id="D5G648"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="68" mass="7692" checksum="5B7424CA8D9B68E8" modified="2010-06-15" version="1">MAKVKGKYSKVNSQTTYRKRMLRGRREGEKAKGAVFLPFPFPPSPTTNRSINQRGFPTYHTCLTLSAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5G689</accession>
    <name>D5G689_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00001658001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430008">
        <property type="protein sequence ID" value="CAZ80032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835875.1">
        <property type="nucleotide sequence ID" value="XM_002835829.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G689"/>
    <dbReference type="STRING" id="656061.D5G689"/>
    <dbReference type="GeneID" id="9183488"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001658001"/>
    <dbReference type="eggNOG" id="KOG3764">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_51_3_1"/>
    <dbReference type="InParanoid" id="D5G689"/>
    <dbReference type="OMA" id="TRTPEVW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17325">
        <property type="entry name" value="MFS_MdtG_SLC18_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050930">
        <property type="entry name" value="MFS_Vesicular_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23506">
        <property type="entry name" value="GH10249P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23506:SF23">
        <property type="entry name" value="GH10249P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="5"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="35"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="98"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="128"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="280"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="320"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="347"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="371"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="1"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="196"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="200"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ80032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="474" mass="50611" checksum="AE7362E076AEE2B8" modified="2010-06-15" version="1">MSLAVFTDIFLYGLVIPVFPFALEKRANVPHDDVQRWISVLLAVYGAGLFIASPVCGYLADISSNRRSSLLAGLLALSGATVLLCVGSNITVLAIGRFLQGVSAAVIWTVGLALIVDTVGKDEVAQSMGVVSVAMSGGIFLAPLLGGIVYDKWVLHKRAVPGIFAMAFGMLTLDIVLRLVIIEKKIAAKYLPKKVESGGPSPGAGPNGMLMRGGKGSEPPKSPSERSDLIQIPKNAHTHTPDNGEGPANDNENSSNDIEAGPTILKKGSRIPPIIRILKYPRLLTALWGTVVQAVIMTSLETTLPLRVRELFDFNATGAGLIFLAVVLPTFLAPLVGWVCDKWGTRWVQTAGFILCCPFFVFLRFPDHNSVSQIVLMCAILALIGIGLVLVLPTMMAEISLFLDEVEEKNPGILGSNGAYAQGYGLFSVAFSAELSFGAEALTIFRKAGFLKDAVGWNNMVWTIGLLPTFLVAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6K7</accession>
    <name>D5G6K7_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Translation initiation factor RLI1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">ATP-binding cassette sub-family E member RLI1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00001816001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Component of the multifactor complex (MFC) involved in translation initiation. Required for the binding of MFC to the 40S ribosome. Required for the processing and nuclear export of the 60S and 40S ribosomal subunits.</text>
    </comment>
    <dbReference type="EMBL" id="FN430010">
        <property type="protein sequence ID" value="CAZ80150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835993.1">
        <property type="nucleotide sequence ID" value="XM_002835947.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6K7"/>
    <dbReference type="FunCoup" id="D5G6K7">
        <property type="interactions" value="1197"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G6K7"/>
    <dbReference type="GeneID" id="9187175"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001816001"/>
    <dbReference type="eggNOG" id="KOG0063">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017344_4_1_1"/>
    <dbReference type="InParanoid" id="D5G6K7"/>
    <dbReference type="OMA" id="MVCIQNG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060255">
        <property type="term" value="P:regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000144">
        <property type="entry name" value="ATP-binding cassette sub-family E member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000152">
        <property type="entry name" value="ATP-binding cassette, sub-family E, member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013283">
        <property type="entry name" value="RLI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007209">
        <property type="entry name" value="RNaseL-inhib-like_metal-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19248">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04068">
        <property type="entry name" value="Fer4_RLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01868">
        <property type="entry name" value="ABCEFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="7">
        <location>
            <begin position="46"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="70"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="297"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00056499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00067238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00082546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ80150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="568" mass="63607" checksum="9D9DD1FE998EA082" modified="2010-06-15" version="1">MADKLTRIAIVSTDKCKPKKCRQECKKSCPVVRTGRLCIEVTPESRIAFISEQLCIGCGICPKKCPFGAIHIINLPTNLETQVTHRYSANSFKLHRLPTPRPGQVLGLVGTNGIGKSTALKILSGKQKPNLGRYDNPPDWNETLAYFRGSELQNYLTKVVEDNIKAVVKPQYVDKLPLAIRGTVRSVSALLKAQCARGDEVMEAMIDILDLRPVLDRDVEQLSGGELQRFAIAVACVQKADVYMFDEPSSYLDVKQRLKAASTIRGLTRPDNYVIVVEHDLSSLQFRIAEAGDEFMVDRSRSFGYPAMDKTLGKFHLHVDKGEFTDSEIIVMMGENGTGKTTFCRMLAGVEAPDGSKKIPKMSISMKPQKITPKFPGTVRELFLKRIRSSFLNPQFQTDVYKPLHIDDFIDQEVKHLSGGELQRVAIVLALGMPADIYLIDEPSAYLDSEQRIIAARVIKRFIMHAKKTAFVVEHDFIMATYLADRVIVFEGEPSIDATATAPQSLLTGCNAFLKSLNVTFRRDQNTFRPRINKLDSQLDQEQKLSGNYFFLEGMLRPPIYSSGYLLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6L9</accession>
    <name>D5G6L9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00002103001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430011">
        <property type="protein sequence ID" value="CAZ80162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836005.1">
        <property type="nucleotide sequence ID" value="XM_002835959.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6L9"/>
    <dbReference type="GeneID" id="9187188"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002103001"/>
    <dbReference type="HOGENOM" id="CLU_3385068_0_0_1"/>
    <dbReference type="InParanoid" id="D5G6L9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ80162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="33" mass="3349" checksum="67DCB1F137F5E36F" modified="2010-06-15" version="1">MLQRRASVIELGGLEGGGIELGILKIGIEGGVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6R3</accession>
    <name>D5G6R3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00002231001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430014">
        <property type="protein sequence ID" value="CAZ80206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836049.1">
        <property type="nucleotide sequence ID" value="XM_002836003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6R3"/>
    <dbReference type="STRING" id="656061.D5G6R3"/>
    <dbReference type="GeneID" id="9187231"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002231001"/>
    <dbReference type="eggNOG" id="ENOG502QQM8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017588_0_0_1"/>
    <dbReference type="InParanoid" id="D5G6R3"/>
    <dbReference type="OMA" id="QIWNEHA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030705">
        <property type="term" value="P:cytoskeleton-dependent intracellular transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008636">
        <property type="entry name" value="Hook_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18947:SF28">
        <property type="entry name" value="GIRDIN, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18947">
        <property type="entry name" value="HOOK PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05622">
        <property type="entry name" value="HOOK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hook C-terminal" evidence="3">
        <location>
            <begin position="90"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="557"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="31"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="95"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="376"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="440"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="568"/>
            <end position="580"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ80206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="638" mass="71946" checksum="86C35B127E3DE027" modified="2010-06-15" version="1">MMEAGGGEDSPDIASEQPRNDSDHFRMEEEMARLVADNEAVKAQNKSLEKRLASLQNDFEESQSHLLVLQDQLSSSVVDRGGYANRTDPIMRSQLDQLQSDVQKLEDVIAEKESTIGQLEGTISGLNRRVENLFPKAEAAIKYKDDLDEANHTIDKLKKSQNVAEKYRKKLEGMGEMEREIKALEQQNTQMKQDLRAGHESSKQVPGLKRTLDQYKKQIDKVEAEYADLLRAKTILEIDRNTLREKAAGAEGQKSKDMERIQNLEEKVRELETGVISKTVEETNGDLDSELTYSTKTKTDLKLQISRLESELSQLKEGTSGTDADNVMLQHMLDDATKAKDKLEQDYLQAHTGKLILEAQLAAINGGSFTEGSEVMLKLRQNLVGAEEQLIDLKRKFAEVDAELSVTKKELTTAKSDLNLVGKDKVEALAELKIINSQELIELRGKHEEAQHKISGLETEIDQKKTLLSTVLLEKDEVSKKLSEQKDIILENEKSNSELRATIATFQGTAEGRDAALEKRVLQLQGKLEDQREKMFKAREHIKKQNVIIRDLKEKVDTAESTTPDATTKSKEEQLAESDRKRRAELEILERENKMIASAWYDQATRLQMNSVALERKGDTPHSWLNRQRAALAKAGGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6S1</accession>
    <name>D5G6S1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">rRNA biogenesis protein RRP5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Ribosomal RNA-processing protein 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00002242001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430014">
        <property type="protein sequence ID" value="CAZ80214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836057.1">
        <property type="nucleotide sequence ID" value="XM_002836011.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6S1"/>
    <dbReference type="FunCoup" id="D5G6S1">
        <property type="interactions" value="1059"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G6S1"/>
    <dbReference type="GeneID" id="9187239"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002242001"/>
    <dbReference type="eggNOG" id="KOG1070">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_287734_0_0_1"/>
    <dbReference type="InParanoid" id="D5G6S1"/>
    <dbReference type="OMA" id="NSHCHEN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05702">
        <property type="entry name" value="S1_Rrp5_repeat_hs11_sc8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05693">
        <property type="entry name" value="S1_Rrp5_repeat_hs1_sc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05697">
        <property type="entry name" value="S1_Rrp5_repeat_hs5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000103">
        <property type="entry name" value="protein RRP5 homolog"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000196">
        <property type="entry name" value="rRNA biogenesis protein RRP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000279">
        <property type="entry name" value="rRNA biogenesis protein rrp5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045209">
        <property type="entry name" value="Rrp5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057301">
        <property type="entry name" value="Rrp5_OB_4th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057302">
        <property type="entry name" value="Rrp5_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048058">
        <property type="entry name" value="Rrp5_S1_rpt_hs11_sc8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048059">
        <property type="entry name" value="Rrp5_S1_rpt_hs1_sc1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23270">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 11 PRE-RRNA PROCESSING PROTEIN RRP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23270:SF10">
        <property type="entry name" value="PROTEIN RRP5 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24685">
        <property type="entry name" value="OB_RRP5_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23459">
        <property type="entry name" value="S1_RRP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="S1 motif" evidence="9">
        <location>
            <begin position="140"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="9">
        <location>
            <begin position="270"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="9">
        <location>
            <begin position="427"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="9">
        <location>
            <begin position="520"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="9">
        <location>
            <begin position="610"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="9">
        <location>
            <begin position="698"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="9">
        <location>
            <begin position="797"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="9">
        <location>
            <begin position="906"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="95"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="186"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="37"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="104"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="187"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00073619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00076674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ80214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1070" mass="116708" checksum="22B0B43A15CB7A07" modified="2010-06-15" version="1">MGSDLKRKRSQDADPGPANKSQTKKLPATANKRLKPEASAAASKPEPAKLSTFRSSKDEEKSFSRGGGSVLTPLEFKQISIDAAKDALFETENAKAKWTKKKSRREEPKKDKTSKKDESKKGEQKGIKAEGLSYKRLLPGTLVLGCVSQISQTDLALSLPNNLTGFVPLTSISELLNKNFEALVRDSDDDEDEDVEENIETAKSESSEDGVDLKSMFQIGPYLRAYVVSSSEPTNSKYSTGSKRFKKRIELSLDPVLTNNGLTTTELVVGCTVQASVTSVEDHGLVMNLGIGNHLKGFLSSKELGKGRSVTDAKEGQVMLCTTIGLSSNGKIVKLSGGLEQKPTKGGKAIGVAGKVMGLVDAKMDFFHASGWEEKDIESKFKVGEKIKAHVIATYSEPRKLALSILPHVLPFTQPIENEPTTILPIATIINTAKALNIEPKIGLFLDVGVPGVPGFVHISRISSDSKIEALSNDSGLYQTDSVHMVRIIGYNSMDGLYLVSMEQKVLDQSFLRVKDIKIGEVVKGTIDRVLHSGRVIVNLAEGITGIVDELHLSDVKLKHPEKKFREGVEVKARVLLTDPPKRKVRLILKKAIVNSDAPIISSYKDTNSGTRSVGTLVKILPNGAIVKFFLDVCGFLPVSEMREAYIQDPHEHSTVGQSVNVHVLSVDPASQKLRVSCKDPNLFGEAHKVTLAKLPPGDVVSGTVVEKSADDLIVEINGLGAEGIRGVLVTGQLTDGSRDKSLGVFKKLRAGQKLDDLLVLEKHEERRSITLSMKPSLVKAAKGGSMISKFEDVNEGEIVRGWVRNTTLQSLFVGFAGGIVGVVYKKDRPAEVQSLPNFGYVKNQSITCRVVYIDPSERRFRLSLNSVKSGEGEAPAVANTAGDTERLTVNPVDARFKHIDDYTSGRLTKAKVVSVQETQLNVKLADNVQGRIDVSLVFETWDAIKDKKSPLASFGKGSVLGVKIIGIHDARNHRFLAITHQKSNTKTPIFELSARPGHIKEEGIERCITKLEDITPNSTWVAFLNNISEECARANILPGIRGRIRILDLSKRSEDTRILTGSMRCQILR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5G740</accession>
    <name>D5G740_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00002332001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 34 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430021">
        <property type="protein sequence ID" value="CAZ80333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836142.1">
        <property type="nucleotide sequence ID" value="XM_002836096.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G740"/>
    <dbReference type="STRING" id="656061.D5G740"/>
    <dbReference type="GeneID" id="9184267"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002332001"/>
    <dbReference type="eggNOG" id="KOG4748">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021434_2_1_1"/>
    <dbReference type="InParanoid" id="D5G740"/>
    <dbReference type="OMA" id="DQDAYIM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000136">
        <property type="term" value="C:mannan polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000009">
        <property type="term" value="F:alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000149">
        <property type="entry name" value="Alpha-1,6-mannosyltransferase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008630">
        <property type="entry name" value="Glyco_trans_34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31306">
        <property type="entry name" value="ALPHA-1,6-MANNOSYLTRANSFERASE MNN11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31306:SF10">
        <property type="entry name" value="ALPHA-1,6-MANNOSYLTRANSFERASE MNN11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05637">
        <property type="entry name" value="Glyco_transf_34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ80333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="303" mass="34340" checksum="CE9422CB0644F84A" modified="2010-06-15" version="1">MHFALPARNHRSSPFPASSVPRAYFCRKRPLYAVVSLAILLLIAFARNSGDGRPAGAPNTVMILLTDKIGYSESYVDKIIKNRGEYAEAHGYGLFIRDVADYPIGEVPNGWARIPAIRHAMKEYKYSTYFWVLDQDAVIMNPSLSLEEHILSPERLDSIMRRGVPVVPPSSIIETYKHVPPERVQLIITQDDEGLQPGSMILKAGSWANYLLDAWFDPIFRYYGFQKAEQHALEHIVQWHPTVLTKMAMIPQKIMNSYPNVPGLGGTYEDGDFLVHLKGCAEGKGRSCEKEFQKYLKKRTLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7A3</accession>
    <name>D5G7A3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00002412001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430023">
        <property type="protein sequence ID" value="CAZ80396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836205.1">
        <property type="nucleotide sequence ID" value="XM_002836159.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7A3"/>
    <dbReference type="STRING" id="656061.D5G7A3"/>
    <dbReference type="GeneID" id="9185294"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002412001"/>
    <dbReference type="eggNOG" id="ENOG502QV8M">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030352_2_1_1"/>
    <dbReference type="InParanoid" id="D5G7A3"/>
    <dbReference type="OMA" id="IRMSFRP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072230" evidence="3">
        <location>
            <begin position="27"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="351"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="372"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="413"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ80396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="450" mass="46952" checksum="E755EF7876563CD9" modified="2010-06-15" version="1">MTLPRSLLSMLPLVLLLLLQSLTASAAYVPGPGHDLFARQNPNCVKDCLVPPCGGCAPNQYCALSVQTCDACPKATCANIQSGGGDSDDDGGSSSPSVGPIVGGVFGGLAAAGIIGFLLYRTFIKKKKNQERISMAATAAEKENDFGMLKSARASTHTVASIASTVRTRASNVIQIAYIPGVTNRSGPSTPSHLVPPIPPLPMMGSPTTSQYPRSPLAGDLQFSADDILRGSMYTVNDNRSSVATTIYGQNAVVSQPNIIRAGKAAVVTVKGGSSLATSIASSPSSTIPPVPALYLNVKGKGRQSTLEKVPPSPAFSVGSTFLNRMNSAKKLNTDLPKGAIGENHSTASFIYDSDDSDDDIKPGQRIKRPQSACSSCITDMDTSSPFSDTNSLAVDDIPGPSNSGQPLAGNTRRLSQSLAQRQSRTLDAMVVSSQRTVSPFDDSNSIEKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7B7</accession>
    <name>D5G7B7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00002430001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GAMAD family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430023">
        <property type="protein sequence ID" value="CAZ80410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836219.1">
        <property type="nucleotide sequence ID" value="XM_002836173.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7B7"/>
    <dbReference type="STRING" id="656061.D5G7B7"/>
    <dbReference type="GeneID" id="9185308"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002430001"/>
    <dbReference type="eggNOG" id="ENOG502S7RW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113002_0_0_1"/>
    <dbReference type="InParanoid" id="D5G7B7"/>
    <dbReference type="OMA" id="DFAAMIW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.30">
        <property type="entry name" value="Dynein light chain 2a, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004942">
        <property type="entry name" value="Roadblock/LAMTOR2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10779">
        <property type="entry name" value="DYNEIN LIGHT CHAIN ROADBLOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00960">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103196">
        <property type="entry name" value="Roadblock/LC7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Roadblock/LAMTOR2" evidence="2">
        <location>
            <begin position="13"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ80410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="137" mass="15359" checksum="27CD8A090A2E1DCB" modified="2010-06-15" version="1">MTTAVPTLSEDDLTSLLNRISQKNTVQSLIILDRSSGAIIRSSGSIAAHRDSESLVGEYAGMVWNFVKSAEEMVCGMDEQDDLRLLRIRTKRHELVIVPGKPYVLYSRLVEYGVSDMFQLFADPRFILVVFHDTPPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7D6</accession>
    <name>D5G7D6_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 16">CDP-diacylglycerol--inositol 3-phosphatidyltransferase</fullName>
            <ecNumber evidence="5 16">2.7.8.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">GSTUM_00002565001</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a CDP-1,2-diacyl-sn-glycerol + myo-inositol = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11580"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="2.7.8.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 16 17">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.11" evidence="5 16"/>
    <dbReference type="EMBL" id="FN430024">
        <property type="protein sequence ID" value="CAZ80429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836238.1">
        <property type="nucleotide sequence ID" value="XM_002836192.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7D6"/>
    <dbReference type="FunCoup" id="D5G7D6">
        <property type="interactions" value="622"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G7D6"/>
    <dbReference type="GeneID" id="9188454"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002565001"/>
    <dbReference type="eggNOG" id="KOG3240">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067602_0_0_1"/>
    <dbReference type="InParanoid" id="D5G7D6"/>
    <dbReference type="OMA" id="GISFPIM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003881">
        <property type="term" value="F:CDP-diacylglycerol-inositol 3-phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006661">
        <property type="term" value="P:phosphatidylinositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1760:FF:000011">
        <property type="entry name" value="Cdp-diacylglycerol-inositol 3-phosphatidyltransferase pis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048254">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014387">
        <property type="entry name" value="CDP_diag_ino_3_P_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15362:SF4">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--INOSITOL 3-PHOSPHATIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15362">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000848">
        <property type="entry name" value="CDP_diag_ino_3_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 16" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="12 16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13 16 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14 16" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="15 16" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 16 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="107"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="149"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR000848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAZ80429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="232" mass="26119" checksum="FFAEBBF0955B1601" modified="2010-06-15" version="1">MPTTNGSSQAMSRSTTTNENIFLFIPNLIGYSRIALAGCSLYFMSYHPHYCTLLYTLSCLLDALDGWAARRFNQSTKFGAVLDMVTDRCTTACLLCFLSSAYTKWAILFQGLISLDLASHYMHMYASLERGAGSHKKVEKKMSRVLNLYYSNNKILFTFCAANELFFLSMYLLSFPQFSIVGNTWSWPWVVAAMTFPICAAKQWINVVQMVKAAGSLAEGDMELRSQAKKQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7W0</accession>
    <name>D5G7W0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00002675001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430032">
        <property type="protein sequence ID" value="CAZ80603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836412.1">
        <property type="nucleotide sequence ID" value="XM_002836366.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7W0"/>
    <dbReference type="FunCoup" id="D5G7W0">
        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G7W0"/>
    <dbReference type="GeneID" id="9181422"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002675001"/>
    <dbReference type="eggNOG" id="KOG4463">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057574_0_0_1"/>
    <dbReference type="InParanoid" id="D5G7W0"/>
    <dbReference type="OMA" id="RYRQFWR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43066">
        <property type="entry name" value="RHOMBOID-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43066:SF21">
        <property type="entry name" value="UBIQUITIN-ASSOCIATED DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="14"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="52"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="87"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ80603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="261" mass="29581" checksum="D9C60F783951D1E1" modified="2010-06-15" version="1">MQQSGFRSAPVSQALLYFIVASSLLASILDIKHYFHIQIIPHLWVHRQVWRIFLWQTCYANAGELLFGALVIYHLRVIERLFGTRKYASFLAYCFVATNIAAPFLLAAVFRPLTFGQMNYLPPGPTPILFAALAQYHAMVPGVYRFRLLTSTSSNGEEGPGITLSDKFYVYLLSTQLAFCQPPGSLLSVAVGWTAGYAWRMDLLPKSKWRIPKWLFKDNQGAEFENLRRRLREGEGVADANQGAGQRPLVRQILDQFRGAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5G833</accession>
    <name>D5G833_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00002790001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL13 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430039">
        <property type="protein sequence ID" value="CAZ80676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836485.1">
        <property type="nucleotide sequence ID" value="XM_002836439.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G833"/>
    <dbReference type="FunCoup" id="D5G833">
        <property type="interactions" value="951"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G833"/>
    <dbReference type="GeneID" id="9184149"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002790001"/>
    <dbReference type="eggNOG" id="KOG3295">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075696_1_0_1"/>
    <dbReference type="InParanoid" id="D5G833"/>
    <dbReference type="OMA" id="IQKNHFR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030684">
        <property type="term" value="C:preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000003">
        <property type="entry name" value="60S ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00499">
        <property type="entry name" value="Ribosomal_eL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001380">
        <property type="entry name" value="Ribosomal_eL13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11722">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11722:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01294">
        <property type="entry name" value="Ribosomal_L13e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="191"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ80676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="214" mass="24719" checksum="5AE56B691788DAA5" modified="2010-06-15" version="1">MAIKHNNQIPNNHFRKQWQTRVRTHFDQPGRKHRRRNARLAKVAAVAPRPIDLLRPVVRCPTIRYNRRVRAGRGFTLEELKEAKIPRKFALSIGIPVDHRRQNTSVESLQANVARLNEYRSKLILFPRRANRPKSGDASKEDVTTATQTRNVAAVFPINPNPGRTEFAERTITDAERERNVFRELRVARADARNAGVREKRQKAKEDEAAAKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8G5</accession>
    <name>D5G8G5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00002854001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430043">
        <property type="protein sequence ID" value="CAZ80808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836617.1">
        <property type="nucleotide sequence ID" value="XM_002836571.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8G5"/>
    <dbReference type="STRING" id="656061.D5G8G5"/>
    <dbReference type="GeneID" id="9186027"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002854001"/>
    <dbReference type="eggNOG" id="KOG0332">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2172906_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8G5"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="7">
        <location>
            <begin position="69"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="22"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="5">
        <location>
            <begin position="69"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ80808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="110" mass="11951" checksum="5A34E6A8F73F7CED" modified="2010-06-15" version="1">MAEYCVSLKSCMGKKSKARVGTAVATHKPSTSAPPPTAGGSGSDESQYKVAVKFADIQGDHNSPFYSVKRFEDLGLSKEILEGIYFMNFKKPSKIQERALPLLLSDPPKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8I7</accession>
    <name>D5G8I7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00002891001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TUBGCP family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430046">
        <property type="protein sequence ID" value="CAZ80834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836643.1">
        <property type="nucleotide sequence ID" value="XM_002836597.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8I7"/>
    <dbReference type="FunCoup" id="D5G8I7">
        <property type="interactions" value="155"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G8I7"/>
    <dbReference type="GeneID" id="9186054"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002891001"/>
    <dbReference type="eggNOG" id="KOG2000">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003736_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8I7"/>
    <dbReference type="OMA" id="MRMMSVC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000923">
        <property type="term" value="C:equatorial microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000931">
        <property type="term" value="C:gamma-tubulin ring complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008275">
        <property type="term" value="C:gamma-tubulin small complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061496">
        <property type="term" value="C:half bridge of mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061497">
        <property type="term" value="C:inner plaque of mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071957">
        <property type="term" value="C:old mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043015">
        <property type="term" value="F:gamma-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051011">
        <property type="term" value="F:microtubule minus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090307">
        <property type="term" value="P:mitotic spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1900">
        <property type="entry name" value="Gamma-tubulin complex, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007259">
        <property type="entry name" value="GCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040457">
        <property type="entry name" value="GCP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042241">
        <property type="entry name" value="GCP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041470">
        <property type="entry name" value="GCP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302">
        <property type="entry name" value="GAMMA TUBULIN COMPLEX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302:SF14">
        <property type="entry name" value="GAMMA-TUBULIN COMPLEX COMPONENT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04130">
        <property type="entry name" value="GCP_C_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17681">
        <property type="entry name" value="GCP_N_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma tubulin complex component protein N-terminal" evidence="8">
        <location>
            <begin position="216"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="domain" description="Gamma tubulin complex component C-terminal" evidence="7">
        <location>
            <begin position="537"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="815"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="895"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="921"/>
            <end position="934"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ80834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="934" mass="103438" checksum="59A131AD157096F5" modified="2010-06-15" version="1">MTEERNQRIDSALSSIVEAIIPANPDEDDEIADERHEAALERARAMIKSHGQPSIVPDVQHISDIIKKRLVRDNKTPEPALKFSTLYTRLLNQRVLSQKWAILYLLHELSDSPKTRPSSSAAGIQDVFPEAGITGTRGVGPVGEHAFSEAFSYMGLHRLPGRDSGRGPRVGGLAGGALGSQWNSFGWSKGDGAMKTKAELLAEKYEDSEPTEQALLSSLPFTMLGLSSSHLPFTTSNVSAPPSGRDISTTQKYAISIPPTLPLPLVSLLHTLAEPSLLYRCLNEFVQSSSESGTDGGLVGQSLKSAIGIELRGYINLVGCLENEIRRAVAAAETTIGVRTAGVTLKRCVVWTREATMGLRLMSLIVEQTGDKKGGQLISTIRSFASSHGDPFVHAFAERLLSHVTRPFYDMLRHWIYDGELTDPYLEFFVIEQPNLPDGGGNKGGASSVWEDKYTIHSPQVPTIMTESFAKKVFLIGKSLNFIRHGCSDSEWVESYSKSTSKELGYGDTARLEMSIDEAYKTTMARLIELMGGKFKLFDHLRALKKYLLLGQGDFIALLMEGLSASLERPANTLYRHNLTAQLEHAIRGSNAQFDSPDVLRRLDARMLELSHGEIGWDVFTLEYKIDAPVDVVVTPYGSRQYLKVFNFLWRVKRVEFALGSSWRRFMAGARGVLKAVEDLVGADWKKARCVVAEMVHFVNQLQYYILFEVIESSWDTLQTAISKPEATLDDLIEAHAKYLQHITHKGLLGSTSSRSSGKGKRAGTDGDDTFMTQLHEILKIMLAYKDAVDGLYGYSVAEYTRRQERGQRIETRTKKGEWGIREADEEGEASGRGSNPEDMMLPSLRQRLKALSGDFQARVVVLLGDLAYQSDMDMRLLGVLLSFNEYYPVVNKRRARGEKEKRNTPRTQPGVKPKTKEGSTGESAAGSGVQEGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8N9</accession>
    <name>D5G8N9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00003061001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430049">
        <property type="protein sequence ID" value="CAZ80882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836691.1">
        <property type="nucleotide sequence ID" value="XM_002836645.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8N9"/>
    <dbReference type="GeneID" id="9186250"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003061001"/>
    <dbReference type="HOGENOM" id="CLU_3360019_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8N9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ80882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="36" mass="4105" checksum="BB2E6AA263D36164" modified="2010-06-15" version="1">MICDVCDASLFKLSRLRVPRYKHSTSTSPPESPILR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8P3</accession>
    <name>D5G8P3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00003066001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430049">
        <property type="protein sequence ID" value="CAZ80886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836695.1">
        <property type="nucleotide sequence ID" value="XM_002836649.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8P3"/>
    <dbReference type="FunCoup" id="D5G8P3">
        <property type="interactions" value="288"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G8P3"/>
    <dbReference type="GeneID" id="9186254"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003066001"/>
    <dbReference type="eggNOG" id="KOG0118">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037639_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8P3"/>
    <dbReference type="OMA" id="LRFHHTM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12400">
        <property type="entry name" value="RRM_Nop6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000376">
        <property type="entry name" value="Putative RNA binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034228">
        <property type="entry name" value="Nop6_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF51">
        <property type="entry name" value="NUCLEOLAR PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="111"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="187"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="58"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="208"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ80886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="253" mass="28464" checksum="9D57ABABB86CC2FC" modified="2010-06-15" version="1">MPTIKESHRKKRDSDKHSENDSRKKRKPKRKISDVDLEDEVEEATPTIPPAAESKEEEDTKAGSAKEEPKPEKRKENKKSKKSTDPNPPTIAGEPAEKQEDGNTTKPAHRFIVFIGNLPYTTTHPQLQSHFSSLQPSSIRLITGKAPPHTCKGFAFLEFTSYDRMKSCLAKYHHSVFLDRKINVELTAGGGGAKSKDRREKLRAKNGRLNDQRKRRTEEEEKLKGEKKAKRAAGGEGDNESGVHMSRRGNIMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8R5</accession>
    <name>D5G8R5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00003034001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430052">
        <property type="protein sequence ID" value="CAZ80908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836717.1">
        <property type="nucleotide sequence ID" value="XM_002836671.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8R5"/>
    <dbReference type="GeneID" id="9186281"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003034001"/>
    <dbReference type="HOGENOM" id="CLU_1628255_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8R5"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00413">
        <property type="entry name" value="Glyco_hydrolase_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38121">
        <property type="entry name" value="GH16 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38121:SF4">
        <property type="entry name" value="GH16 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ80908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="163" mass="17941" checksum="F44E06DE720EA883" modified="2010-06-15" version="1">MTSQDTTDGTQAEKLKKRKTPTAQIKYDDWHTHRMDWVHGESSWFVDGEHFLDKKYGVPTVASSFIMNLWSDGGVWSGNMTKGKSAYAEIEFVEMAFNTTGDDRGSANSKVRRATRNKCDRICVIDDVAVVGTPEGAKGGAFKKAVNPWSLGVAMLVAGVLSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8S0</accession>
    <name>D5G8S0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00003043001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EBP2 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430052">
        <property type="protein sequence ID" value="CAZ80913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836722.1">
        <property type="nucleotide sequence ID" value="XM_002836676.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8S0"/>
    <dbReference type="FunCoup" id="D5G8S0">
        <property type="interactions" value="319"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G8S0"/>
    <dbReference type="GeneID" id="9187267"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003043001"/>
    <dbReference type="eggNOG" id="KOG3080">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036007_3_1_1"/>
    <dbReference type="InParanoid" id="D5G8S0"/>
    <dbReference type="OMA" id="RETMFHR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000280">
        <property type="term" value="P:nuclear division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008610">
        <property type="entry name" value="Ebp2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13028">
        <property type="entry name" value="RRNA PROCESSING PROTEIN EBNA1-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13028:SF0">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN EBP2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05890">
        <property type="entry name" value="Ebp2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="79"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="309"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="22"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="79"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="113"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="341"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="362"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ80913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="383" mass="42962" checksum="AE51C141F6EDEDB8" modified="2010-06-15" version="1">MGKSKLKTALLRYQSRDPIREKQKRISRRQPKTLRKRGGKGEQEGGEDSEGNGAPEMVDISNLAMEGVLKFAINNAEEIPAESDSEEEDGVEDSDFSNGGEEAEEEEAEDSDIPLSDVSVSDLDSDTDHLPYQKLTTNNALALTASLARISLPHTLPFSETFLISTPEPIAILDIHDDLTRELTFYKQALSAANEGRARILAEGGQFSRPVDYFAEMLKDDEHMGKIRQKLLDEAAEKRAAQEARKQRDLKRFGKKVQVAKLQERERGKREMLERVKIFKRRRQDVETEATTEADMFNVALEEVINGHRGSTRDGINNKRKKKDQKYGFGGKKRFVKSTDAKSSGDLSGFSNKKNKAQFAGVKKRKPAMRLGKSKRTSGSGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8U1</accession>
    <name>D5G8U1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">2,4-dienoyl-CoA reductase [(3E)-enoyl-CoA-producing]</fullName>
            <ecNumber evidence="3">1.3.1.124</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00004837001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a (2E,4E)-dienoyl-CoA + NADPH + H(+) = a 4,5-saturated-(3E)-enoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:45912"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:85101"/>
            <dbReference type="ChEBI" id="CHEBI:85493"/>
            <dbReference type="EC" id="1.3.1.124"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a (2E,4Z)-dienoyl-CoA + NADPH + H(+) = a 4,5-saturated-(3E)-enoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:61892"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:85099"/>
            <dbReference type="ChEBI" id="CHEBI:85493"/>
            <dbReference type="EC" id="1.3.1.124"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="1.3.1.124" evidence="3"/>
    <dbReference type="EMBL" id="FN430053">
        <property type="protein sequence ID" value="CAZ80934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836743.1">
        <property type="nucleotide sequence ID" value="XM_002836697.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8U1"/>
    <dbReference type="FunCoup" id="D5G8U1">
        <property type="interactions" value="238"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G8U1"/>
    <dbReference type="GeneID" id="9186443"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004837001"/>
    <dbReference type="eggNOG" id="KOG0725">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_2_1"/>
    <dbReference type="InParanoid" id="D5G8U1"/>
    <dbReference type="OMA" id="MQAHVCA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008670">
        <property type="term" value="F:2,4-dienoyl-CoA reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009062">
        <property type="term" value="P:fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05369">
        <property type="entry name" value="TER_DECR_SDR_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045017">
        <property type="entry name" value="DECR2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43296">
        <property type="entry name" value="PEROXISOMAL 2,4-DIENOYL-COA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43296:SF2">
        <property type="entry name" value="PEROXISOMAL 2,4-DIENOYL-COA REDUCTASE [(3E)-ENOYL-COA-PRODUCING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00026117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ80934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="297" mass="31019" checksum="844BBF175D2E668B" modified="2010-06-15" version="1">MSAAIKKHMSPIWAPGIFEGKVVLCTGGAGSICSTQVAALILLGANASIIGRRKDVTEAKAAELQTLRSGSKVLGISTDVRDYSALVTTVKRTVEELGRLDYVICGAAGNFLATVDNLSVNAFKSVIDIDVLGSYNTVKASLEELKKTRGKIIFVSATLHYTGSPFQAHVSAAKAAIDALSRVLCVELGPYGITSNCIAPGPIAGTEGMARLSRPEVASGAERAIPIQRLGHVHEIADATIYLLSPAGDYVSGDVIVVDGGAWHRQGASAFPYPESVTRKGVVEGVKGAKKAKDSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8W8</accession>
    <name>D5G8W8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">Probable electron transfer flavoprotein subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00004875001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 7">The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </cofactor>
        <text evidence="7">Binds 1 AMP per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="7">Binds 1 FAD per dimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the ETF beta-subunit/FixA family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430053">
        <property type="protein sequence ID" value="CAZ80961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836770.1">
        <property type="nucleotide sequence ID" value="XM_002836724.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8W8"/>
    <dbReference type="FunCoup" id="D5G8W8">
        <property type="interactions" value="474"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G8W8"/>
    <dbReference type="GeneID" id="9181244"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004875001"/>
    <dbReference type="eggNOG" id="KOG3180">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060196_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8W8"/>
    <dbReference type="OMA" id="EINQPRI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009063">
        <property type="term" value="P:amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01714">
        <property type="entry name" value="ETF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000011">
        <property type="entry name" value="Electron transfer flavoprotein subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000049">
        <property type="entry name" value="ET-Flavoprotein_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014730">
        <property type="entry name" value="ETF_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012255">
        <property type="entry name" value="ETF_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033948">
        <property type="entry name" value="ETF_beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21294">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN BETA-SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21294:SF8">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01012">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000090">
        <property type="entry name" value="Beta-ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00893">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01065">
        <property type="entry name" value="ETF_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Electron transfer flavoprotein alpha/beta-subunit N-terminal" evidence="8">
        <location>
            <begin position="26"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00070315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ80961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="253" mass="27390" checksum="C5B76AD417A2AE15" modified="2010-06-15" version="1">MTSLRILVPIKQVIDYAIKPRVNATRTAVVTSGVKMSMNPFDELSIEESVRLRERNRDAVRDILAFSAGPAKAADTLRTAMAMGADRALLQGEGSDLEPLFVAKLLREVVHKEGCNMVICGKQSIDDDSGQTGQMLAGLLGWGQATQAAKVDVQGSEVVVTKEVDGGEEVVRVKLPAVVTTDLRLNEPRYATLPNIMKAKKKPIEKKTLADFSVEFGKRLETLKVVEPPPRQGGGKVDSVDGLISRLKELGAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5G945</accession>
    <name>D5G945_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00003121001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430054">
        <property type="protein sequence ID" value="CAZ81038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836847.1">
        <property type="nucleotide sequence ID" value="XM_002836801.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G945"/>
    <dbReference type="GeneID" id="9184325"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003121001"/>
    <dbReference type="HOGENOM" id="CLU_2279491_0_0_1"/>
    <dbReference type="InParanoid" id="D5G945"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ81038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="102" mass="11137" checksum="2F9631622325AEEF" modified="2010-06-15" version="1">MVCPPSPSALMAIDHGAPLSLFPSLLSSPLQTRHDVSQHCLRIGFMLQKSKISLQHTPSSNLHFSKITHTVSNSFHTSPTTSSLASSTLFYCLLRHSVNPKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>D5G972</accession>
    <name>D5G972_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00003179001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430056">
        <property type="protein sequence ID" value="CAZ81065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836874.1">
        <property type="nucleotide sequence ID" value="XM_002836828.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G972"/>
    <dbReference type="STRING" id="656061.D5G972"/>
    <dbReference type="GeneID" id="9184351"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003179001"/>
    <dbReference type="eggNOG" id="ENOG502S0PE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057546_1_3_1"/>
    <dbReference type="InParanoid" id="D5G972"/>
    <dbReference type="OMA" id="HTIFDFR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026893">
        <property type="entry name" value="Tyr/Ser_Pase_IphP-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126:SF1">
        <property type="entry name" value="TYROSINE SPECIFIC PROTEIN PHOSPHATASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13350">
        <property type="entry name" value="Y_phosphatase3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="1">
        <location>
            <begin position="148"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="288" mass="32307" checksum="D857019E56180AF5" modified="2010-06-15" version="1">MASRYTPAYTEETLAVLSRPPWKLVQGIANFRDIGGYPLRDGSGSVRRAYIYRSAEPSRITEEGKILLRDTMKVSHTYDLRSVPELKRMEKETPIIEIEGITRNFVPVFKDVDYSPENVALRFKSYSSGDLTEAYRCILQAGGKTAYRSILSHVLTRPDEPLLIHCTVGKDRTGVITALILLLAGVGEEVIAEEYALTTLGMGSVKEAIWDHLIKQPLFAKGDAEAIQGLENMLSSRKDTMLKTLKIIDEEFGGVRGYLEKQCGFSEEEIEIIRKNIVCDEPPCFPLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9G3</accession>
    <name>D5G9G3_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">26S proteasome regulatory subunit RPN2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00003388001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the proteasome subunit S1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430061">
        <property type="protein sequence ID" value="CAZ81156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836965.1">
        <property type="nucleotide sequence ID" value="XM_002836919.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9G3"/>
    <dbReference type="FunCoup" id="D5G9G3">
        <property type="interactions" value="1342"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G9G3"/>
    <dbReference type="GeneID" id="9182907"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003388001"/>
    <dbReference type="eggNOG" id="KOG2062">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002323_0_0_1"/>
    <dbReference type="InParanoid" id="D5G9G3"/>
    <dbReference type="OMA" id="IMFGRQE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034515">
        <property type="term" value="C:proteasome storage granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004175">
        <property type="term" value="F:endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042176">
        <property type="term" value="P:regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000017">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016642">
        <property type="entry name" value="26S_Psome_Rpn2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002015">
        <property type="entry name" value="Proteasome/cyclosome_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048570">
        <property type="entry name" value="PSMD1_RPN2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040623">
        <property type="entry name" value="RPN2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10943">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10943:SF2">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13646">
        <property type="entry name" value="HEAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01851">
        <property type="entry name" value="PC_rep"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18004">
        <property type="entry name" value="RPN2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21505">
        <property type="entry name" value="RPN2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015947">
        <property type="entry name" value="26S_Psome_Rpn2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0647">Proteasome</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="26S proteasome non-ATPase regulatory subunit 1/RPN2 N-terminal" evidence="9">
        <location>
            <begin position="5"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="26S proteasome regulatory subunit RPN2 C-terminal" evidence="8">
        <location>
            <begin position="740"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="796"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="891"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="798"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="892"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="925"/>
            <end position="952"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR015947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ81156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="952" mass="104462" checksum="B947D2F3643A6835" modified="2010-06-15" version="1">MPGLTSASGLIGLLDEPDQQLKCYALQNLDKLVDQFWAEIADSVSKIEVLYEDDQFSERNLAALVASKVYYHLGEYNESMMFALGAGDLFDITAKSEYVETIISKFPQKADPRLQDIVERMFRKCYETEDYKPAIGIAIEARRLDVVEEGINLASRRSKDKKGKEKSCEEEKDGGVELMEYVLEISMNVVQEIGLREKLLRLLVNLFLSQSSPDYFSISKCVIHLNDTSLAAKILQELVQKEDIKSLLVAYQIAFDLDSSATQEFLQKTGKYYRSIRQILRGTKSIEIALEFLFRNNHTDMSILNKIKDSLEARNSIFHTAVTFSNAFMNAGTTSDGFFRANLEWLSKAVNWSKFTATAALGVIHRGNLSQGLKLLEPYLPQEGVSTGSPYSQGGSLYALGLIYANHGTDVLEFLRKQFKGTADEMVQHGGALGLGVAGMATGNQGIYDELKTVLWSDSAIAGEAVGLAMGLVMLGTGSSASLEDMIQYAHETQHEKIIRGLAIGMALIMFGKQEAADDLMNTLLDDPDPTLRYGGIMTIAMAYCGTGSNKAIRQLLHVAVSDVNDDVRRVSVMSLGFILFRKPNAVPRMVELLSESYNPHVRYGASLALGISCAGTGLDEAIDLLEPMIKDPTDFVRQGALISMAMILVQQNDQMNPKVASLRKTFEGMVGEKHEDAMAKFGAALALGIIDAGGRNVTIGLQTQTGSLNLPAIVGMAVFTQYWYWFPLTHFLSLSFMPTGLIGLDDELNVPDFKFWSNTRPSLFDYPPKVEESAEKAPEKIATAVLSTTVKARQRAKRSEKEKAAKEAAAKGEDAMETDHTVSTPKRAEKEKVGYELGNMSRVLPEQLKYISFISDGRYEPVKKPTGGILLLLDRTPSEPIRLMELKARKATAPTATGPGHTVGSINDGPGTGGAAAAVNVLNAEDDEGDEDAPPPEDFEYITDGETEEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9G6</accession>
    <name>D5G9G6_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00003392001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS12 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430061">
        <property type="protein sequence ID" value="CAZ81159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836968.1">
        <property type="nucleotide sequence ID" value="XM_002836922.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9G6"/>
    <dbReference type="FunCoup" id="D5G9G6">
        <property type="interactions" value="896"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G9G6"/>
    <dbReference type="GeneID" id="9182910"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003392001"/>
    <dbReference type="eggNOG" id="KOG3406">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110343_1_0_1"/>
    <dbReference type="InParanoid" id="D5G9G6"/>
    <dbReference type="OMA" id="DLGQWVG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990145">
        <property type="term" value="P:maintenance of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000005">
        <property type="entry name" value="40S ribosomal protein S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000530">
        <property type="entry name" value="Ribosomal_eS12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047860">
        <property type="entry name" value="Ribosomal_eS12_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11843">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00972">
        <property type="entry name" value="RIBSOMALS12E"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01189">
        <property type="entry name" value="RIBOSOMAL_S12E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="5">
        <location>
            <begin position="6"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ81159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="120" mass="13554" checksum="1F2A82F89835E49E" modified="2010-06-15" version="1">MSVEEALKGVLKHALIHDGLARGLREASKALDRRQAHMAVLNENCEEEPYKKLVEALCSEHKIPLIKIADGKKLGEWAGLCVLDREGNARKVVNCSCVVVRDWGEESQERNVLLQYFQTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GA38</accession>
    <name>D5GA38_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00003527001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cytochrome c oxidase subunit which plays a role in assembly of respiratory supercomplexes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Associates with the respiratory chain complex III/complex IV supercomplex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RCF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430072">
        <property type="protein sequence ID" value="CAZ81381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837190.1">
        <property type="nucleotide sequence ID" value="XM_002837144.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GA38"/>
    <dbReference type="FunCoup" id="D5GA38">
        <property type="interactions" value="70"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GA38"/>
    <dbReference type="GeneID" id="9187079"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003527001"/>
    <dbReference type="eggNOG" id="KOG4431">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087356_1_0_1"/>
    <dbReference type="InParanoid" id="D5GA38"/>
    <dbReference type="OMA" id="QRWIREL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097250">
        <property type="term" value="P:mitochondrial respirasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007667">
        <property type="entry name" value="Hypoxia_induced_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050355">
        <property type="entry name" value="RCF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12297:SF3">
        <property type="entry name" value="HIG1 DOMAIN FAMILY MEMBER 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12297">
        <property type="entry name" value="HYPOXIA-INDUCBILE GENE 1 HIG1 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04588">
        <property type="entry name" value="HIG_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51503">
        <property type="entry name" value="HIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="HIG1" evidence="9">
        <location>
            <begin position="6"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ81381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="153" mass="17547" checksum="D758FE5C180BFC51" modified="2010-06-15" version="1">MSSCPLPSSFDSDSDFTEASRLSKFTRRLKEEPLIPIGCLITVYALYQSSRAIRAGDKARTNRMFRARIYGQAFTVAAMCAGSFYWARDREARKASEAKIAEAKARKQQAAWIRELEVRDREVRELQERVKRYTTVKEPTTARETKAKVEEGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>D5GA67</accession>
    <name>D5GA67_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00005156001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WAPL family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430075">
        <property type="protein sequence ID" value="CAZ81421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837230.1">
        <property type="nucleotide sequence ID" value="XM_002837184.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GA67"/>
    <dbReference type="STRING" id="656061.D5GA67"/>
    <dbReference type="GeneID" id="9185703"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005156001"/>
    <dbReference type="eggNOG" id="ENOG502RZXD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_301720_0_0_1"/>
    <dbReference type="InParanoid" id="D5GA67"/>
    <dbReference type="OMA" id="NNKPKAC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039874">
        <property type="entry name" value="WAPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022771">
        <property type="entry name" value="WAPL_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22100">
        <property type="entry name" value="WINGS APART-LIKE PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22100:SF13">
        <property type="entry name" value="WINGS APART-LIKE PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07814">
        <property type="entry name" value="WAPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Wings apart-like protein C-terminal" evidence="3">
        <location>
            <begin position="514"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="125"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="259"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="326"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="380"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="433"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="259"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="335"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="391"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ81421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="990" mass="109013" checksum="49C49FB1829517CA" modified="2010-06-15" version="1">MGGSVLVADPVQQEQHHPQQRKSSSGVNGKSTFARAYLRNITPPAPPTQSSKELAAKKLDSADIFDFEVSSDVEEVPVIPTRKGGSRGLVKTKKKPAITRPTAVAGFDVPSTESDIPERKIALSKKRSYKKGGGVTVPEPKRRKSLKDSDEEMVLSVAKGKGAISAALPKGKKDLKGDKITSTAKVTKNSTVLKVTKHSTFEVSIPYTLDGQPSAKPTLSKSIVKSEAQFLTKKQEQEKKLKPLIKPKSAPDLIERKCAPREKVREPLPKPTAEKESAKKSCKTTLKTIAAPRVQKAVPAMLPGLKAMHVPIEQPVLVRERSVGPNISAPVDKKVGKKGKAKAAPRGNKRLSEELSDGGEFDIPLIAELRPVRRRRLIDTLGGDTRGSRAGTRSTSVTTDSSGDGDSFTQDAQYPQFQQLSESQNGSSQLQDLFQFGSQGPKPPTLKPTYTAGRIGPKVTYAQQRSFKADENMSEQNLFGIPLMMMPSSYSGRTGVNFDEGLEEEPLATAKGMMKNIHELRETGSNNRFLDDVEDFFGDIEGKNVPLGTKRSGYIGLACKMVDKTFVRNFRANNFDTRLLKRVAKQTDEVICFTLAFMIARLLWEDSTVQVLSEVRANGALDMLARTLDFERDIKVVSKDKKTNMSKAAQGMVVELRTLVDKSTVFPQCKPRVYSAQIMGLMVLETVIRRLRATGIQDRIVSLDVLGKLVTMVKPFITPTKAGKALDLLVLELLVSALEAWACGLDVEIETAFSKDELATLTDIIPTIISQAVTGDNGKFHVGDFDVKDILLLTLRLNINMTNDRIEACDALASSDLIPILIELIRSRFGILDGQLEEVPRLVNLDLLVLGLGLLNNFAEMSIPGRCSVLRKGETGVLLLDILVRLFLARFSKTEDADSLEESQSNVGFGYLAILLGNLCQDTEIRIAVRNQLPNKSLNILYESMEVFIAHHRKVDELKDESDIFYQLGEAHCAFTERLERVVRRLKEGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAI7</accession>
    <name>D5GAI7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00003606001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430078">
        <property type="protein sequence ID" value="CAZ81530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837339.1">
        <property type="nucleotide sequence ID" value="XM_002837293.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAI7"/>
    <dbReference type="GeneID" id="9187470"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003606001"/>
    <dbReference type="eggNOG" id="KOG0719">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055868_0_1_1"/>
    <dbReference type="InParanoid" id="D5GAI7"/>
    <dbReference type="OMA" id="WLDLWSK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056453">
        <property type="entry name" value="HTH_DNAJC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052594">
        <property type="entry name" value="J_domain-containing_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44144">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44144:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23302">
        <property type="entry name" value="HTH_DNAJC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="19"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="181"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="217"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="217"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="285"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="329"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ81530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="367" mass="41515" checksum="B3CDAFAB43CB0822" modified="2010-06-15" version="1">MSDNESSSSSGTPPPITKSPYEILEVPTTSTAAEIRSAYRKLALTVHPDKVPEGERDAAKTAFQELTFSYGVLSDETRRKRFDETGSTSETAEGGFDWKAFYKAQMADLITTESLRKFKEEYQGSEEERQAVITAYQACEGSMDKIIESVMCSDVLEDEERFRQIINEALDAGQVKSFRSFTHENKASKKRRRDKAEAERREAEAYAKELGIHDELFNNKKAKAEEVEDEHREEGADKGSKKSKGKKTRKVDPAEDISGLRNLIQKRSNNRMEEMMSSLEAKYLPKSKSKKKAATNSKRKKATDLDNEDEEAELAPPEPTEEQFLAARARMEGSKKPKTTKEARGKAKTVKEDSADTELRRSKRVKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAM5</accession>
    <name>D5GAM5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00003684001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I NDUFA12 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430079">
        <property type="protein sequence ID" value="CAZ81568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837377.1">
        <property type="nucleotide sequence ID" value="XM_002837331.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAM5"/>
    <dbReference type="STRING" id="656061.D5GAM5"/>
    <dbReference type="GeneID" id="9187735"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003684001"/>
    <dbReference type="eggNOG" id="ENOG502S4P5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067876_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAM5"/>
    <dbReference type="OMA" id="GENTNHR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045271">
        <property type="term" value="C:respiratory chain complex I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052618">
        <property type="entry name" value="ComplexI_NDUFA12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007763">
        <property type="entry name" value="NDUFA12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32470">
        <property type="entry name" value="ADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX ASSEMBLY FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32470:SF2">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX ASSEMBLY FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05071">
        <property type="entry name" value="NDUFA12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="151"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="159"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="178"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ81568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="211" mass="23611" checksum="316A7B5396346287" modified="2010-06-15" version="1">MTSKAQGNILTRIYYAWKSLKLPWRRKIFVGMDLEGNSFWEIPISVHGRVRRIVAYRDPKRDLVDYKLPPQWTQWLRHNRVAPTIAELHGDVARQAQLKQLAAAADARWNSKPSLLNAAPHPPTDSVLESNTKFCNDSGLQRREEMCRGRGETGVGFGGKDKEGYRPAGESVDRAHGARGGEAWGGKAGGEFKVGEWDPNAGLPKKRPGRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAV4</accession>
    <name>D5GAV4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Cobalamin-independent methionine synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Methionine synthase, vitamin-B12 independent isozyme</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">GSTUM_00005323001</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="14">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the vitamin-B12 independent methionine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.14" evidence="4"/>
    <dbReference type="EMBL" id="FN430086">
        <property type="protein sequence ID" value="CAZ81647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837456.1">
        <property type="nucleotide sequence ID" value="XM_002837410.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAV4"/>
    <dbReference type="FunCoup" id="D5GAV4">
        <property type="interactions" value="511"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GAV4"/>
    <dbReference type="GeneID" id="9187815"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005323001"/>
    <dbReference type="eggNOG" id="KOG2263">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013175_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAV4"/>
    <dbReference type="OMA" id="KVMKGML"/>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00082"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003871">
        <property type="term" value="F:5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019280">
        <property type="term" value="P:L-methionine biosynthetic process from L-homoserine via O-acetyl-L-homoserine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03311">
        <property type="entry name" value="CIMS_C_terminal_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03312">
        <property type="entry name" value="CIMS_N_terminal_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.210:FF:000002">
        <property type="entry name" value="5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.210:FF:000003">
        <property type="entry name" value="5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.210">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00172">
        <property type="entry name" value="Meth_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013215">
        <property type="entry name" value="Cbl-indep_Met_Synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006276">
        <property type="entry name" value="Cobalamin-indep_Met_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002629">
        <property type="entry name" value="Met_Synth_C/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038071">
        <property type="entry name" value="UROD/MetE-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01371">
        <property type="entry name" value="met_syn_B12ind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003556">
        <property type="entry name" value="PRK05222.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30519">
        <property type="entry name" value="5-METHYLTETRAHYDROPTEROYLTRIGLUTAMATE--HOMOCYSTEINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08267">
        <property type="entry name" value="Meth_synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01717">
        <property type="entry name" value="Meth_synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000382">
        <property type="entry name" value="MeTrfase_B12_ind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51726">
        <property type="entry name" value="UROD/MetE-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 14" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Cobalamin-independent methionine synthase MetE N-terminal" evidence="17">
        <location>
            <begin position="4"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="Cobalamin-independent methionine synthase MetE C-terminal/archaeal" evidence="16">
        <location>
            <begin position="443"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="15">
        <location>
            <position position="712"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>5-methyltetrahydropteroyltri-L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:58207"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>5-methyltetrahydropteroyltri-L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:58207"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="448"/>
            <end position="450"/>
        </location>
        <ligand>
            <name>L-homocysteine</name>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="448"/>
            <end position="450"/>
        </location>
        <ligand>
            <name>L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="501"/>
        </location>
        <ligand>
            <name>L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="532"/>
            <end position="533"/>
        </location>
        <ligand>
            <name>5-methyltetrahydropteroyltri-L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:58207"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="578"/>
        </location>
        <ligand>
            <name>5-methyltetrahydropteroyltri-L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:58207"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="616"/>
        </location>
        <ligand>
            <name>L-homocysteine</name>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="616"/>
        </location>
        <ligand>
            <name>L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="659"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="661"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="670"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="683"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="744"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000382-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000382-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000382-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF08267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAZ81647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="774" mass="85462" checksum="A63830CBF99C9C07" modified="2010-06-15" version="1">MVTSSVLGFPRMGGNRELKKATEAYWAGKISAEELIQTGKNLRASHWKIQKDAGAGTIASNDFSFYDHVLDHIQLFNAVPNRYRKYNLPALDEFFAMGRGLQRAATESSPAVDVPSLEMVKWFDSNYHYVRPTLQDNQTFSLAADPKPVREFLEAKAQGVNTRPVLVGPVSFLHLGKPDRGASVNPITLLENLLPVYIELLTKLREVGAETVQIDEPILVLDLPPRTKAAFKTAYARIAAAGVSIPKITVATYFGDIVHNIDVLSSLSAVSAVHVDLVRNPEQLDTVTAALAPGQILSAGVVNGRNIWKTDFGEAIAIVQKAVNALGADRVVVATSSSLLHTPHTLASEKNLPAEVADWFSFASEKAREVAVIARAVTEGAASVKAELDANAASMHSRSTSARTNDPAVKERQSKVTAAMYERQSSFTQRIAAQQTHLSLPLFPTTTIGSFPQTKGIRVARQKFGKGDISAAEYESFIEKEIELVVREQEALGLDVLVHGEPERNDMVQYFGERLTGYVFTNFGWVQSYGSRCVRPPIVVGDISRPAPMTVKESKYAASITSKPMKGMLTGPITCLRWSFPRDDVHQSVQAEQLALALRDEVVDLETAGVNVIQVDEPALREGLPLRSGKERDAYLDWAVKSFRLATTGVKDVTQIHSHFCYSEFQDFFHAITALDADVLSIENSKSDAKLLKVFEDEAYPRHIGPGVYDIHSPRVPPQEEIRARIEEMLQFLKPEQLWINPDCGLKTRQWPETRAALDIMVKAAKEFRAKYQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAZ0</accession>
    <name>D5GAZ0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">LSM complex subunit LSM4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LSM4</name>
        <name evidence="12" type="ORF">GSTUM_00005366001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Binds specifically to the 3'-terminal U-tract of U6 snRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">LSm subunits form a heteromer with a doughnut shape.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430086">
        <property type="protein sequence ID" value="CAZ81683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837492.1">
        <property type="nucleotide sequence ID" value="XM_002837446.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAZ0"/>
    <dbReference type="STRING" id="656061.D5GAZ0"/>
    <dbReference type="GeneID" id="9187851"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005366001"/>
    <dbReference type="eggNOG" id="KOG3293">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1116438_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAZ0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097525">
        <property type="term" value="C:spliceosomal snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01723">
        <property type="entry name" value="LSm4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000024">
        <property type="entry name" value="U6 snRNA-associated Sm-like protein LSm4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034101">
        <property type="entry name" value="Lsm4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027141">
        <property type="entry name" value="LSm4/Sm_D1/D3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23338">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 9" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="11">
        <location>
            <begin position="117"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="196"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="203"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="235"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ81683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="249" mass="26828" checksum="E8C0F3C409A59A33" modified="2010-06-15" version="1">MRKQGNTKQDSKRRPQALNANVPVGSLSKGWLVGEGTSLSTSRRTNEAQRNLHPALIQIRSGATTPTYLLPSPSQQRNSTSFTTTFPFITHSSVISPQLFSRSSISSSERNGARSPLPLSLLNTAQGHPMLVELKNGETLNGHLVNCDTWMNLTLKEVVQTSPEGDKFFRLAECYVRGNNIKYLRVPDEIIDLVKETQTQQSRDGHGGRGGNDRGRGGYRGDRGNRGGRSRGRGRGGGGGGGGGGGSNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5GB65</accession>
    <name>D5GB65_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00003764001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430087">
        <property type="protein sequence ID" value="CAZ81758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837567.1">
        <property type="nucleotide sequence ID" value="XM_002837521.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GB65"/>
    <dbReference type="FunCoup" id="D5GB65">
        <property type="interactions" value="378"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GB65"/>
    <dbReference type="GeneID" id="9181468"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003764001"/>
    <dbReference type="eggNOG" id="KOG0008">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000572_1_1_1"/>
    <dbReference type="InParanoid" id="D5GB65"/>
    <dbReference type="OMA" id="RFINYYR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040240">
        <property type="entry name" value="TAF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022591">
        <property type="entry name" value="TAF1_HAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13900">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13900:SF0">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12157">
        <property type="entry name" value="DUF3591"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription initiation factor TFIID subunit 1 histone acetyltransferase" evidence="4">
        <location>
            <begin position="413"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="75"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="310"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="859"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="966"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="991"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1064"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="19"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="321"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="872"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="991"/>
            <end position="1010"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ81758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1084" mass="121217" checksum="9FFC0D11C30BEC08" modified="2010-06-15" version="1">MASITATNDAPRSPRGDPEADAEGESDDGIEYNEDNDEKIINGIISGTITAGDDPHAFGFGDTVGKLDDAIDYEDISDDDLPSEGEPSGNNNIGGKEEVGEDGIAGDEFDELFNDIGDEAPIAGADASGELGGLEGVDLDFMAQEGLVGTSEMDVGQTANEDMMMGLDMDLGAPTEGPSAEKRATTQELVKRYFPELDLKARLSFNDLFGPKPQRLAMGPPKPPKVIVPTKLAIVFAPSDETVFNKPYTGRNMAKKAEERPGIIIVPQEPEDSEESDADVEDPIDWNSPLERGIMMACNDWESKMDMIMGPTPPPTPPSEVLEDDDEDAEEANRRPLKRARLASSFLDGLPDSWLADDVVLDGNLSAIASHVTLDLNDPHLLVDIRQPNEIRPTKRIGGEFKRRINKDLAQRYNISNDEAYDLLKGNLQNKVRSAIGQLDIEHSMPALRLQSPYYKTKLSTKEARSFHRPGLHFNVNYEIRFSKIKARKKKTYRGKDVQSVLKITKDLSLMDGCNFILLEYSEEYPFVMSNFGMGSRLINYYRRKGPDDESRPKCEIGETQVLMSQDRSPFWNFGTVDPGETVPTLYNNMVRAPIFKQNPKPTDFLVIRNTQANQSHYYLRNIPNLFVVGQLLPVTDVPGPHSRKVTTAAKNRLKMVCYRVVKRKPEKAIILKDIAEHFPENNEMRQKMKEFMSYNKPKGVWEMNAGEVVPDESVIRTMVKPEDLCLLEAMQVGVRHLADSGYSKTVDDEVDDDDKEGMSLEQQLTPWVSTKNFINATSGKAMLQLHGEGDPSGRGEAFSFVKTSMKGGYKPVGESVEEKMDKQRLKELGGHAYNVARQQQMYEAAIDQIWRAQHRSLSSQVEPEMPDTEFEDTHNDHHEDLLEEGRTPRSEAVPPSPWLNNVDVDETMSQFSRFSATNRRNKVLRIQRKFRGPNGGVETRSEIIRDPKVIRQYLQRRRELDAEKTQVENLVPTGNEDEDQQSRKRLVQELKRLERNKERREARERDKGKRVGSFAGDAMSPESAATPGSPMPSVSMQKCKNCGQVGHIRTNKKVCPKLNGQWAELGMSPPDGNTPAPVGTPGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBA8</accession>
    <name>D5GBA8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glutaminase</fullName>
            <ecNumber evidence="2">3.5.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00000394001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutaminase PdxT/SNO family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.2" evidence="2"/>
    <dbReference type="EMBL" id="FN430090">
        <property type="protein sequence ID" value="CAZ81801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837610.1">
        <property type="nucleotide sequence ID" value="XM_002837564.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBA8"/>
    <dbReference type="FunCoup" id="D5GBA8">
        <property type="interactions" value="181"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GBA8"/>
    <dbReference type="MEROPS" id="C26.A32"/>
    <dbReference type="GeneID" id="9181512"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000394001"/>
    <dbReference type="eggNOG" id="KOG3210">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069674_0_0_1"/>
    <dbReference type="InParanoid" id="D5GBA8"/>
    <dbReference type="OMA" id="GMIMLAD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903600">
        <property type="term" value="C:glutaminase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042823">
        <property type="term" value="P:pyridoxal phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008614">
        <property type="term" value="P:pyridoxine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01749">
        <property type="entry name" value="GATase1_PB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01615">
        <property type="entry name" value="PdxT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002161">
        <property type="entry name" value="PdxT/SNO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021196">
        <property type="entry name" value="PdxT/SNO_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03800">
        <property type="entry name" value="PLP_synth_Pdx2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31559">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31559:SF0">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01174">
        <property type="entry name" value="SNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005639">
        <property type="entry name" value="Glut_amidoT_SNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01236">
        <property type="entry name" value="PDXT_SNO_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51130">
        <property type="entry name" value="PDXT_SNO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="93"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="189"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="191"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="60"/>
            <end position="62"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="154"/>
            <end position="155"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR005639-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR005639-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ81801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="223" mass="24503" checksum="16EDB91D9FF5BC3F" modified="2010-06-15" version="1">MSGNDSITVGVLALQGAFSEHLELLKKAAEQIETPKKIGFISVKTPVDLEKCSALIIPGGESTTMALIAERSNILEPLREFVKVHRRPTWGTCAGMILLSEAANRTKKGGQALIGGLSVRVNRNHFGRQVESFDAPLDLSFLGNDEAPFRGVFIRAPIVESILPKEECCTNASSIVAVRQGNVVGTSFHPELTGDLRMHKWWLGEILKDLTRREELLHAQENH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBH1</accession>
    <name>D5GBH1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00000497001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430095">
        <property type="protein sequence ID" value="CAZ81864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837673.1">
        <property type="nucleotide sequence ID" value="XM_002837627.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBH1"/>
    <dbReference type="GeneID" id="9184438"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000497001"/>
    <dbReference type="HOGENOM" id="CLU_1157110_0_0_1"/>
    <dbReference type="InParanoid" id="D5GBH1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="240" mass="26110" checksum="B5FECF82B84DDA05" modified="2010-06-15" version="1">MNPEPVHNRRLSVEDAEDDEVLPSYEDVIQGGSSATIVAALPARLRYRTPSPRPGSRSQQRISLPDPSTAPSSASPQQYSSLLGPSFAPTIVASPSLATVTPPHSRPIGNGNSPQELRASRSPQPKYSSLLPPASGPTGPPPNYNVTTSSLPEPPRSREGKERDKAPRTQRWMFGKKASQAYHEGHLEKKYAPSKYEGVPLEYVVDRFGNRKRELKFEMDIGVSITGLVRVGVKSSRFGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBK8</accession>
    <name>D5GBK8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00005681001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ82014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837823.1">
        <property type="nucleotide sequence ID" value="XM_002837777.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBK8"/>
    <dbReference type="FunCoup" id="D5GBK8">
        <property type="interactions" value="538"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GBK8"/>
    <dbReference type="GeneID" id="9185449"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005681001"/>
    <dbReference type="eggNOG" id="KOG0581">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_63_23_1"/>
    <dbReference type="InParanoid" id="D5GBK8"/>
    <dbReference type="OMA" id="VGTMYFM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000935">
        <property type="term" value="C:division septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004708">
        <property type="term" value="F:MAP kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000196">
        <property type="term" value="P:cell integrity MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050850">
        <property type="term" value="P:positive regulation of calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000294">
        <property type="entry name" value="Map kinase kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000263">
        <property type="entry name" value="MAP kinase skh1/pek1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050915">
        <property type="entry name" value="MAP_kinase_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47448">
        <property type="entry name" value="DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE DSOR1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47448:SF5">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINAE MKK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="146"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ82014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="438" mass="48452" checksum="8584B2C257CBF0A6" modified="2010-06-15" version="1">MSAPAQLLRPANPHNGPRVPRLGLSIPPQPGSRSVGAPQLVGPARAPPQLRLATPGNPSANGIQPVDQRRAQAYASPVNTVNNQYSALGYAMGLKNQVRGSPETAVPRMASQEGANKKTIDWDNDNLDVEDLDDEGWKKASEDGRIVELGGLGEGAGGAVTRCMLKGGKTLFAMKIITADPNPEIRKQIFRELSFNKECNSPYICRYFGAYTDLSSATISIAMEFCEGGSLDSIYREVKKLGGRTGEKVLGKIAEGVLEGLTYLSGRKIIHRDIKPSNILLCRDGQVKLCDFGVSGELIGSKGNADTFIGTSYYMAPERIQGFSYTITSDVWSLGVTLLEVAQHRFPFGEESDSTNARYNLIDLLTYIVQHPIPELKDEPNLKIKWSHNFKYFIACCLEKDTQRRASPWRMLEHPWIMEMKNKKVNMANFLSQVWDWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBL8</accession>
    <name>D5GBL8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00005693001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ82024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837833.1">
        <property type="nucleotide sequence ID" value="XM_002837787.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBL8"/>
    <dbReference type="STRING" id="656061.D5GBL8"/>
    <dbReference type="GeneID" id="9185459"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005693001"/>
    <dbReference type="eggNOG" id="ENOG502QZJZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011099_0_0_1"/>
    <dbReference type="InParanoid" id="D5GBL8"/>
    <dbReference type="OMA" id="VDDNKWM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050987">
        <property type="entry name" value="AtrR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910:SF25">
        <property type="entry name" value="ABC-TRANSPORTER-REGULATING TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910">
        <property type="entry name" value="TRANSCRIPTION FACTOR PDR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="4">
        <location>
            <begin position="24"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="109"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="677"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="752"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="127"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="677"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="694"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="704"/>
            <end position="730"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ82024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="824" mass="91891" checksum="A3DFC80D465F9A56" modified="2010-06-15" version="1">MFNPGQASPDDGAGDPKRRRIARACDMCRKKKIKCDGKLPACTHCMNYKTECVFTLVEKKRNPPKGAKYIEGLENRLGRMESLLKLSGLLPEGDGKTDLGDLERRLQDQAQQPGGAGSVKTPSNIGRSSSESMRETPSSSALQSAVNSPGLHKEEDEVEALSDQMCSLVTNNCGETRFIGSSSGFSIFSPKGIQWVNEKTGDKSFQHMVMEATSSESKWGMWKPDIFGPLFEQRGTNPLPSRELAAILVQDFFQNFNTMFPLFHRPTFEHLFEKQYSDDPPNHSGWYGAFNMLLAISFRLRITHATEPDAEHSISHVQKSWLYFQNAATVLTELLLKNTDLLSIQAILGMALYLQGTANPQPSYFLIAAAVRISNSIGMHRRGNSFGLNQVEVEQRRRVFWIMYLLDKDMALRSGRPPCINDDDISVELPDEDPTDGVGNVPLRVGKGKVNLFRLMSSFAVIESKVYMQLYSAKAAKQSDGELLNTIGDLDKELEEWKETVPADYRPENEISMDDGPLVLHLVMLHFAYYNCLTTIHRMSIHHGYWTSRLSNYAIAGLSARPLNPRVFSSAALCVSAARASIHLIKYINKHDYPCIWLIMYYPVSALVTLFANVLQNPQDPRARSDLRLMNVVVEFLQTVQKEDESENAKQNTSSIQRMLAVCGEFHRISQLVLDKAEKESMTRRKRKNDESKPPSSGAQYPVSSARSASSSATAAPSTPPANAASPPTSILNQDMTSQLYGEFGTTFSPTNPMSWLNDMGNPSPAQNFPLSPSPSHNPLPPLISSSFQQPFVPQDLWQMPMTFEWDWADVSSMAWTEQGPSGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBQ4</accession>
    <name>D5GBQ4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00005522001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ81904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837713.1">
        <property type="nucleotide sequence ID" value="XM_002837667.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBQ4"/>
    <dbReference type="STRING" id="656061.D5GBQ4"/>
    <dbReference type="GeneID" id="9182941"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005522001"/>
    <dbReference type="eggNOG" id="ENOG502S4TA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078549_0_1_1"/>
    <dbReference type="InParanoid" id="D5GBQ4"/>
    <dbReference type="OMA" id="PLIRCDV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12467">
        <property type="entry name" value="RRM_Srp1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000123">
        <property type="entry name" value="Pre-mRNA splicing factor (Srp1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034403">
        <property type="entry name" value="Srp1p_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050907">
        <property type="entry name" value="SRSF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23147">
        <property type="entry name" value="SERINE/ARGININE RICH SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="7"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="87"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="108"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="118"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="164"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ81904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="185" mass="20909" checksum="0545A9AA4DC03DFA" modified="2010-06-15" version="1">MSGRGGTTLYVTGFSSGTRARDLAYEFERYGRLVRCDIPAPRTASSRLFAFVEYESRRDADDAYYEMHGRRIGRDDLLKIEWARTPPSASWRFDGGRDAPRGGARRGGSPRRGRSVSRGRDSPRRSERRGERDARSRSPGRKGDDDVRRPDDDRERSPLAANGDDAHHRKDSPLHDDLDTADLAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>D5GC15</accession>
    <name>D5GC15_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00005762001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430098">
        <property type="protein sequence ID" value="CAZ82058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837867.1">
        <property type="nucleotide sequence ID" value="XM_002837821.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GC15"/>
    <dbReference type="GeneID" id="9181327"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005762001"/>
    <dbReference type="eggNOG" id="ENOG502SD8H">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090064_1_0_1"/>
    <dbReference type="InParanoid" id="D5GC15"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="170" mass="19094" checksum="3908567199D9D76D" modified="2010-06-15" version="1">MPTQTEEARTSLTHENSSPANPAPTLQPLVDDQASYTALRYRASLGVLVVAPALVILPPRKMDLFTFGLGASWLYCLGEVANQHGRDPWRGFKRYEQFEKEKLEGRDMGGRVAETNGELVGAGGWRGEKVVRDVRPKEAGRSISEIIIERVWDVWEQKDRDQEEGKKGKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCA8</accession>
    <name>D5GCA8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00000656001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430108">
        <property type="protein sequence ID" value="CAZ82151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837960.1">
        <property type="nucleotide sequence ID" value="XM_002837914.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCA8"/>
    <dbReference type="GeneID" id="9184829"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000656001"/>
    <dbReference type="eggNOG" id="ENOG502S2Q1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109881_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCA8"/>
    <dbReference type="OMA" id="CKNAANS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054246">
        <property type="entry name" value="DUF6973"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22322">
        <property type="entry name" value="DUF6973"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072649" evidence="2">
        <location>
            <begin position="22"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="DUF6973" evidence="3">
        <location>
            <begin position="56"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ82151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="171" mass="17860" checksum="3FEF1EEFD8EF3182" modified="2010-06-15" version="1">MKFLSSLSLLLVAAASTGTSALPTSSTSTSTLVVRGYSASGAETSYCLWPSRWSVCSKVKDHAGEALTAAEQNFPLSSLHNGKGDAFRHCYWNARMTVDMGKGTAKTFADLHEEGGDGPAAEKDMDLSNNASGRRFGEEAKNGGGSDGDKYARALTKCKNAANSGALKVLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCG2</accession>
    <name>D5GCG2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00005877001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
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        <lineage>
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            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838014.1">
        <property type="nucleotide sequence ID" value="XM_002837968.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCG2"/>
    <dbReference type="GeneID" id="9186387"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005877001"/>
    <dbReference type="HOGENOM" id="CLU_3175714_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCG2"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ82205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="47" mass="5090" checksum="9C9410766DD1EDC6" modified="2010-06-15" version="1">MPVRKTRSAPPSLHPHDFGFWGFFSSFSFARFCCLGAAEGGRVVNGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCK4</accession>
    <name>D5GCK4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00000687001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430112">
        <property type="protein sequence ID" value="CAZ82247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838056.1">
        <property type="nucleotide sequence ID" value="XM_002838010.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCK4"/>
    <dbReference type="STRING" id="656061.D5GCK4"/>
    <dbReference type="GeneID" id="9186430"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000687001"/>
    <dbReference type="eggNOG" id="ENOG502QRBS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022730_2_1_1"/>
    <dbReference type="InParanoid" id="D5GCK4"/>
    <dbReference type="OMA" id="GHIIETA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847:SF213">
        <property type="entry name" value="DEPENDENT OXIDOREDUCTASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847">
        <property type="entry name" value="SARCOSINE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="1">
        <location>
            <begin position="65"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="482" mass="51854" checksum="5AFF116B74CCF037" modified="2010-06-15" version="1">METTIPVSQLTKLVLPNFDSHLQSLASALSTAPRQPVSNPTSSFWLSDPPPVFTHQSPTFPASADVVIIGSGITGTAAARTLLSNSPDLRVAMLDAREACSGATGRNGGHIKPNPWELFPALAAVLGREKARKVTEFRRGILDKMIAVADAEGVTGECQIRKVEAADVHFDAESWAAAKTCLVVYLEEFPEEIGNWRVYEGEEARQKFSTPHAKGIITGPAGALWPARLIHPILTRLLSMHPTFTLDTSTTVTSITPPDSPTSPYIVHTPRGDLKATHILHATNGYTANLLPGLTGALFPVRGTMTAQSPPTKCHLHENRSWQFSWDQGYDYLTTLPDRTIMFGGGLRQALKTEIGTVDDSIVELCTTIHLSGFLPAIGLGTGVQKIWTGIMGFSGDMLPWIGEVPPEISGRQQGIGKEWVAAGYTGDGMCVAWGAAEEVVARILGLEGGVGVIEEMDVTVERVESAKRLERLVGAFFGREG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCW2</accession>
    <name>D5GCW2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00000806001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430126">
        <property type="protein sequence ID" value="CAZ82355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838164.1">
        <property type="nucleotide sequence ID" value="XM_002838118.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5GCW2">
        <property type="interactions" value="646"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GCW2"/>
    <dbReference type="GeneID" id="9183822"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000806001"/>
    <dbReference type="eggNOG" id="KOG2208">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003293_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCW2"/>
    <dbReference type="OMA" id="DHAGQQV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02394">
        <property type="entry name" value="KH-I_Vigilin_rpt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057778">
        <property type="entry name" value="KH_Vigilin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054548">
        <property type="entry name" value="SCP160-like_KH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22952">
        <property type="entry name" value="KH_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24668">
        <property type="entry name" value="KH_Vigilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="5">
        <location>
            <begin position="153"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="5">
        <location>
            <begin position="224"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="5">
        <location>
            <begin position="308"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="5">
        <location>
            <begin position="387"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="5">
        <location>
            <begin position="547"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="5">
        <location>
            <begin position="629"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="5">
        <location>
            <begin position="717"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="5">
        <location>
            <begin position="784"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="5">
        <location>
            <begin position="870"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="5">
        <location>
            <begin position="946"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="5">
        <location>
            <begin position="1027"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="5">
        <location>
            <begin position="1100"/>
            <end position="1210"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="5">
        <location>
            <begin position="1215"/>
            <end position="1284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="54"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1135"/>
            <end position="1160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="75"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ82355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1302" mass="142263" checksum="CCC9C88D9534AC9A" modified="2010-06-15" version="1">MSKQSNGTNGAPPAEGSATQITVVAPVDENSVEAAKELSTAERLMQQHAIVAQREATTSQAKNEDATVTDAPIAKVEKRFPKQNEKPEVRGLDVSSDEAFPTLGGGSGKPNLARVPFGWGAGMAAPMANGNGYNGESATASASAFTPAVQGSSGQSTIYLLPTQKRHLSELRKPPNELAKDIMRSTGTKIEMQQTSTQTTVFIISGTEANRQKANREIHKEMSAKKTVTIEVPAIVRPHIIGKAGIKVQELESMTATRIKVPQLQASDGPPAEYDDEDTIDITIEGDEYGVTIAVHKIKEIVAERTITVTTRILDIPGELYPFIQGPHRKFIEEYETKVQRVQVPDYFYSAKNQPFSPPSGPIVITGEKRAVQELRAEIERKATEFKATFHSVPVPVNKAKHRFFTSNRGQLIHQVLATAGCTVILPEGAKVDSLIVYGPSDKIPQGYQEVMKASNAYQQVAIDVCKAYPKAPGGPKLPARDITRYLDHTGEFQSLQSEFDLEITTPSRDELYDLDKPCVIHIIGKSKDGVNGVKAKIQELYSKLTPGHILRFEVEPLHHRLIVGRDGRGVRRITERFLGLCLLLPEDVGEAEIVLIYDGPSQDPEEISKSLEELKEHVLSLVEAQKFDVAEKIITIPHGFHAKVRGEENTTLNALNPTSVHVYFGALKARLGNPAAAASPGDEDNITIRGAKAGVDNLATAIKQFVASIPEDGNIPTVSEQFTYPTELSAQLIGAKGANVNKLRAELGVEINLKEGQGEIRGIQVAVDAARRKLSLQIKELSDKDIKHLKIPQEFHSTIIGSGGNSVRKLETRYGVRIMFPKPGRDKDADGADVPAKQDANEVIVKGGKDGVSKARDEILALLEYEKGKSFTTSIPITGRGLEFMFKNASKEIKQLRDDSSVRLDIPQRDAIGKDEQAVIKIRGTEDEVKNVKTILSKIVKEAEQTTQRSISVEKKYHRSLIGPGGHTLKEIVVGAGGPHDKHALARTVRFPNQESDSSEIIVQGSSAVVDKIVAAIEEIVEQKNSEITGTVEVAPEKHRHLIGREGSIRRELEAKFRVTIDIPRQKPGQTNTSPDVKITGIAEDVDKAKDHILEMIKEPESETIEIPTRLYHAIADGGQLIRRLQRDFRVSIDHNGQQRPPKPEEPEPDLSGSFPLITDDEEDASQGFTWEVVESDNQGGEEGDFPWVIRGKLEQIAKAKAEIAKAVALAQQQSYIGYLVLPDSSKFRLIIGPSGSQVEKIRRDTSCRIAVPRSQDVGEPIVMHGSKEGVERAKEIILGLVNNGDSNGHGRRRGNGNGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDV0</accession>
    <name>D5GDV0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00006283001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic release factor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430153">
        <property type="protein sequence ID" value="CAZ82693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838502.1">
        <property type="nucleotide sequence ID" value="XM_002838456.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDV0"/>
    <dbReference type="FunCoup" id="D5GDV0">
        <property type="interactions" value="1269"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GDV0"/>
    <dbReference type="GeneID" id="9184488"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006283001"/>
    <dbReference type="eggNOG" id="KOG0688">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035759_2_1_1"/>
    <dbReference type="InParanoid" id="D5GDV0"/>
    <dbReference type="OMA" id="RCNGSEE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018444">
        <property type="term" value="C:translation release factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003747">
        <property type="term" value="F:translation release factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.60:FF:000001">
        <property type="entry name" value="Eukaryotic peptide chain release factor subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.960.10:FF:000001">
        <property type="entry name" value="Eukaryotic peptide chain release factor subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000006">
        <property type="entry name" value="Peptide chain release factor subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.960.10">
        <property type="entry name" value="eRF1 domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.60">
        <property type="entry name" value="eRF1 domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042226">
        <property type="entry name" value="eFR1_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024049">
        <property type="entry name" value="eRF1_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005141">
        <property type="entry name" value="eRF1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005142">
        <property type="entry name" value="eRF1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005140">
        <property type="entry name" value="eRF1_Pelota-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004403">
        <property type="entry name" value="Peptide_chain-rel_eRF1/aRF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03676">
        <property type="entry name" value="aRF1_eRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10113">
        <property type="entry name" value="PEPTIDE CHAIN RELEASE FACTOR SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03463">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03464">
        <property type="entry name" value="eRF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03465">
        <property type="entry name" value="eRF1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01194">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55481">
        <property type="entry name" value="N-terminal domain of eukaryotic peptide chain release factor subunit 1, ERF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="eRF1/Pelota-like N-terminal" evidence="5">
        <location>
            <begin position="9"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ82693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="445" mass="50109" checksum="C05AF67851F36037" modified="2010-06-15" version="1">MSLPQANDMEKNIEIWKIKKLIKSLTEARGNGTSMISLIIPPKDQVSRVGKMLSDEYGTASNIKSRVNRLSVLSAITSTQQRLKLYHKVPPNGLVLYCGEIITNEGKERKINIAFEPFKPINTSLYLCDNKFHTEALSELLESDSKFGFIVMDGKGALFGTLSGNTREVVHGFSVALPKKHGRGGQSALRFSRLRDEKRHNYVRKVAESAVQNFITNDKVNVSGLILAGAADFKTDLAQSELFDPRLQAKIIKIVDVSYGGDLGFIQAIELAEETLGNVKFVQEKKLISKYFGELAQDTGKVCYGIDDTLRAMELGAAETVIVFENLDVTRWILKSSDGNLITLHTNKIQEQDRQKFMDKSSRQEMEIVEQTSFLEWLAEKYHDFGATLEFVSDRSGEGNQFVKGFGGIGALLRYKVNFEQLHELEDEDEYYDGNFLTVIWSYLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>D5GE57</accession>
    <name>D5GE57_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00006388001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430164">
        <property type="protein sequence ID" value="CAZ82800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838609.1">
        <property type="nucleotide sequence ID" value="XM_002838563.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GE57"/>
    <dbReference type="FunCoup" id="D5GE57">
        <property type="interactions" value="338"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GE57"/>
    <dbReference type="GeneID" id="9183132"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006388001"/>
    <dbReference type="eggNOG" id="KOG1617">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051314_0_3_1"/>
    <dbReference type="InParanoid" id="D5GE57"/>
    <dbReference type="OMA" id="PEAMMTA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043337">
        <property type="term" value="F:cardiolipin synthase (CMP-forming)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032049">
        <property type="term" value="P:cardiolipin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050324">
        <property type="entry name" value="CDP-alcohol_PTase-I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14269:SF60">
        <property type="entry name" value="CARDIOLIPIN SYNTHASE (CMP-FORMING)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14269">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--GLYCEROL-3-PHOSPHATE 3-PHOSPHATIDYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="105"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ82800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="151" mass="16831" checksum="8E3A246C1AD2689C" modified="2010-06-15" version="1">MADKTLMTILTVTLAMKGLLPVWLAGIILSRDIGLAISAIYFRWISLPPPKTMARYWDFSLPSAEVRPTTISKLNTALQLGLIGACMAHPFMVTMGPGWDIALRAMQYTVATTTVWSGLSYVYSKDAVRILKRPREPEDRANPDDNKKKMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>D5GF18</accession>
    <name>D5GF18_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00006700001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430197">
        <property type="protein sequence ID" value="CAZ83111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838920.1">
        <property type="nucleotide sequence ID" value="XM_002838874.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5GF18">
        <property type="interactions" value="128"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GF18"/>
    <dbReference type="GeneID" id="9188649"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006700001"/>
    <dbReference type="eggNOG" id="KOG1826">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000249_0_2_1"/>
    <dbReference type="InParanoid" id="D5GF18"/>
    <dbReference type="OMA" id="LPMGYHE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05392">
        <property type="entry name" value="RasGAP_Neurofibromin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039360">
        <property type="entry name" value="Ras_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023152">
        <property type="entry name" value="RasGAP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001936">
        <property type="entry name" value="RasGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194:SF142">
        <property type="entry name" value="NEUROFIBROMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13716">
        <property type="entry name" value="CRAL_TRIO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00616">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00323">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00509">
        <property type="entry name" value="RAS_GTPASE_ACTIV_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50018">
        <property type="entry name" value="RAS_GTPASE_ACTIV_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-GAP" evidence="4">
        <location>
            <begin position="964"/>
            <end position="1157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="267"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="267"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ83111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="2351" mass="263422" checksum="BB67A1B81AA23297" modified="2010-06-15" version="1">MSQRLKFLRTTASDDSDASGLQFIAHMWINQEKLSLIIKEISGSFLPLRKPAQNMLASLLPETIHRWIEAHPKDFVDLHMNNGRLEKGVDILFDIAASLADGAKRRAIIWPLQTALILLLPDVFWTLDIRSEQRGSLSKKVAFLNDLRRSLRLPKNSDIAASCLIIMCRAGSLFPAEPDGALLSFALDVQNEVTEEIFRRQQFGEDLSVDRDIMIKAFVSSARLGVDSVVGNLLPRCLDKNSPITFKITVFAGVAVLASQNIASTRHANTSSTNGSSLAGGTLNSQTRHKNLSTDSIGSSELLYQLLELLKIRPLLMYENLDAGAEEWDRLGDRMISSLLRLIYDDDEFVRHSAVVFARRLLSPEAFQMTTDMRLGQGAISVLNFFWKATLLVHGYLETRVSIISSRKVTFASGTQVSSPIKAEFLEKDITEGITDIPERTIANVSLEVSFLVLLCSSDLDICSITTHAIALLCEEGRLTENPDDLARSNLTVMRNFPVYSELSLQTFRITGPVAFQKRLRKLLTRMSMPGPGILTAWEAVFTRWRTLSKHILSPGVANAGGIDDGLFAEWRNYSGFLASIAGCCIADLPQQLRADDSTTSGLRWIDRLASDGDQMSLLERFMKQCLQLLVCKLVNVRENIREVLGTELNPRLYLQLFRSLETEFSILFDPGFKDAQSESPSRTLFAEQACSLLKTIVERLEDAQDMFLTVDLGALTLSLARYLNTLKEDYTTLRVKIRMCQLVELVARKKELVNLRQDIRVRNNLLQILSEWMPRPAKPNYPINPRKDELIRPQRDLDRACLKALVNLLHRLPLQPPETAHDSADVVDARSQMFCSYFTLFLSLLDSSQLESDRRRELQTVSLAKDDASSFQDLAIQALSNLLSANVDVGLKFSLEIGYHDDLQTRTAFMHVLTNILTQGTEFGALGDSAIGEKYEKLIDVSSLLVNDLQFALALCDSCPSSEVDEMSIALLNIFDSRGLGLTLLKELIEQEVANTESESELLRRNCVATKMLSVFAKWKGSEYLRNVLQKVLQRVIVSSDKLGLELDPTRTPSTEELHRNELQLRYITKVFIDEICKSGNIAPNSFRWICHTITACVTSRFPEAKFTAVGAFIFLRFFCPAIVAPDSEGLVNNIPNKEMRRGLLLIAKIVQNLANNVLFGAKEPYMIPLNDFLTANICVVTAFLREISSLPKKQEPVVAQESFDFGSSVALHRFLYDHWEIVRQKLIFQEKHKARLNGEKSFSADAGPSNLQLSITKFSGLISTLGAPPLDISLGRPTLTGNIQPAYSRYQHFMLRNSGRSVESILAARIVYDGGETKDNIPVICFVLRNINIGTVDPDLLIYCYLKVCFRMWHKPFAVLIDATCYSLNNEMPDELYKKIDNLMPPEMVKNYSRLYIYNMNTAFRKFFRRQLRYAVRDDTNAWNPNNIDFIMLGSVVELQQHFNLGSLHLPKETMSFLSDTRYMYHHITRLSKTKGKIEVVFKIGAQYIQITPTKKQEIVAGLKMSATINDIFRLNDVEEANASFHTDEENAFGIKTDNGKLTMFFSSPKKNEIVQTLKSSKSKYSKDNRPSKLNERTIRPEDVPGTLLNISLMNIASYDQYLRLAAYNLLCALCQAFRFNLDRQFVSAKGLSIPTDSVILIVGVSEKLAAAEPQLTFDFLSEFFVGWEKSHPQQRPLNILYMAPWLANLHSQVLMGGEDPERGKERLAAIARKMIDITVREPRLYTSFQQNAWCIISKDEGLLDVFLDELVKSAMNFGFGSEGAETIGSIASSFGTPTIRGKIIARIRKVLNRSSLRPMRHLVDNIVWNEICVLLRLCLAISFDSRVQAQMYLPELFHIITMVVNCGSIPVRSTVHSLLVNTVHSICVSFPLEDAKLTQLKGILASLSEPKLCLLFSLNRPTSRDALAIQEQRGSETTTATSMELITNLLLEIVSVAAPSTDMANIWRARWMSLVASTAFQSNPAIQSRAFAVMGCLAREDVDDDLLYQVLVALRGALARYSESGDHEMLTSIVTSLTKMMENLQPTSRYLYQLFWLAMSLVRVGHGLVFTCSASLLEATLKTIASSGEFKDNRMVSVLLQGRSQLEEATAAIDDIYNIKFNSESFHFAVCATLTKGLQDPSTKPSALRTLTAFLEVISANTPESSQWGTDMPVPPYLSMVLTRATSTSEMKEVLWITGIALADEDATMDSGSGGSGGSGWDTCAKLTPMIDIMSEKNLLLIGALAVVDFRSCEESIQKATLALFTRMAERRPEVLLLLYDHLLEYLDEVLNSSHNPMLLKEANALMCVVSMDSRFANRGSAAKETLAYRLARWGLSGIWTATSFRSTKEFEKRCAGLTDRLIEVCIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>D5GF37</accession>
    <name>D5GF37_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00001829001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">P-body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat EDC4 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430198">
        <property type="protein sequence ID" value="CAZ83130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838939.1">
        <property type="nucleotide sequence ID" value="XM_002838893.1"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GF37"/>
    <dbReference type="GeneID" id="9187997"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001829001"/>
    <dbReference type="eggNOG" id="ENOG502R9YM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003635_0_0_1"/>
    <dbReference type="InParanoid" id="D5GF37"/>
    <dbReference type="OMA" id="YVNPFDQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031087">
        <property type="term" value="P:deadenylation-independent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.100">
        <property type="entry name" value="conserved c-terminal region of ge- 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055393">
        <property type="entry name" value="Beta-prop_EDC4L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045152">
        <property type="entry name" value="EDC4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044938">
        <property type="entry name" value="EDC4_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15598">
        <property type="entry name" value="ENHANCER OF MRNA-DECAPPING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15598:SF5">
        <property type="entry name" value="ENHANCER OF MRNA-DECAPPING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24106">
        <property type="entry name" value="Beta-prop_EDC4L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="EDC4-like protein pdc1 beta-propeller" evidence="7">
        <location>
            <begin position="539"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="175"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="461"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="989"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1058"/>
            <end position="1132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1350"/>
            <end position="1375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="10"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="66"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="88"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="103"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="122"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="186"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="202"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="317"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="367"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="470"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="999"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="1356"/>
            <end position="1370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF24106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ83130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1511" mass="164335" checksum="892D07C632ACF43C" modified="2010-06-15" version="1">MSPPHGDPLAGPSGQQQQHQHQQNQQSAHQSQPPQQQHSPQAARSGIFPAPPFHLGNILPPPPPSQHFSRNNLSEPLSSPGLHQSQPGFPPYHPETLPPQQPPSQQQSQQLPTSQPAVAEVVNNNSRNPSGPQMFSQNTPPPYANPMSPSVDGANASRTASLLQLLKFSQAVPVAPTSEISQPVATPSPRPSVSSPPPISSHTQLQQSQPSHSGGVSASDLVASLLGRPSEPPGGSGLRQENIAPEMPTEPTVSKGPSDRLSAVNPQNLVLQLLGRPKPSQDDLPSTGPATAPRSNPPMPERHMSQGPGDFGSPKQSPIPPEPRPMSVPPAAPSKSPPPSHGVSMPFSSPTPSKGLFTHVNPFEQLHATSPRNRTPRSSTTPLPANNGKSGLGTREVKAENVPLPYSSTSSFAQSENSPVSATIEAEAAKNASTLDQALKNEEEMKDNLNSAVVENAVNASEQREMTESPIGQQENETPPAANGTSTQETFGDEDYENIPPARGHHEKEEDDNYQSASEAHGSPVLVYNFPMKPFSSITINPSTAPRPKFPISKISDIARMPRLFDQLDRNLIAASHQFIVYAISKSHGRGGIRVLRQFDGKDKVLMKDSTDRTFNVTIGNGDRVLGTGISGAVVWAELGDFDGPDWNNMFVFPPSEEQGQSNGVLKSRARKTCRQAEVFAIGRGKTISIIHAPTAKAYAQGRKNNEVASKKYLAEHSRTIDTGKASKDFAFSDDDSVIISIDKAGKLKLWDVQELIDFSSNDAYNSLQPAPAPQPPIVLTNPILMLSAVAAGEPYRATSVMFLDKFRPYHKCLALRYVIVGMKQNHTLQLWDLALGRPVQEINFPQESDTDALCSVAYHPTTGIIVVGNPTRNSIYFIHLSAPKYNLPPMSQAQYIRGLAVKDSSIPKPDATAILSGLREYSFASKGQLMSLDILDGDAQGSLPPLFELYVAHSKGMTALNVFKEDLGWDGKNSAQNPVDAVKEGICTLSAMSPPPPPEKDRDSDNSNRSEKSSKCEKGIEKVEKPKVPEKVNSPEEVESAEMADKVADLQFGATQGLKSAPPENAPKGPAKSRSVSPGARAPDQGEEKPAVHNGNTKRKRKDKGNTQQSGPSGPSDEKSDKTKSVSSELAKTLNKEITDLYRRIDEDKRIQQAAGDAKQDAILRLLSSTLTDNVEQVIGRIVMTNIQQSVLPAISSVTSAAVERKLGESVSRSLAATLPAELHAIVPEAVKKIFGQPEFMARIGEAISRPLAYSMEQEISKTICRSLLPAFKQLSDETTSKLVADFDRKHNETIQALERVHVQDTQRIDQLTNTVKSLVDVVQAMAKSQAEFQDQVQRAQAEYVQAYEQTGEPEPAPRAPTPPPPPTPDQLEAEETERFLRNGRYEEGTIKWLQSKERQAELFDEVVVRYRYDFLPNLSQLVLLSVSAAVSIKFENKVYERLSWLEGVLAVLDPMDPEIHEICHRIMAVVVQRLEQLYMSTAENNMADPILRKIPPIARRARELSSMAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>D5GF50</accession>
    <name>D5GF50_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00001878001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430206">
        <property type="protein sequence ID" value="CAZ83143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838952.1">
        <property type="nucleotide sequence ID" value="XM_002838906.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GF50"/>
    <dbReference type="STRING" id="656061.D5GF50"/>
    <dbReference type="GeneID" id="9188005"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001878001"/>
    <dbReference type="eggNOG" id="ENOG502QRTD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031065_0_0_1"/>
    <dbReference type="InParanoid" id="D5GF50"/>
    <dbReference type="OMA" id="KNHREWI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ83143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="389" mass="45350" checksum="D6A9F62E039F3E6A" modified="2010-06-15" version="1">MACTLSFRRRLLAPFLAVLLTVGLIIGYRAHVLPQERVVISPPSIPFRTPIPDPKPAVEIFPDAVNGRFPQIHVVNQPPKFHHPTPLFIGFTRNWPILQQSVVSYITAGWPPVDIYVVDNTGTMDSNELGLLTLQNPFFVDYPRLRALGVNIITAPTLLSFAQLQNFFLYTAIRKKWAHYYWSHMDSAVLSHEEQQPYRSLYQRIIENWNSMNKTEKWAIRFFAYDHLALVNVAAYKDIGGWDTQIPFYITDCDFHARLHMKNYTTTDENVGHIFDVGGTVPDLSVFYPETPSEPLNSERFKHLHKLLEDIQRSKSEAKYGRNLWQSEQRGGKGEPFYRNPVGFEKAMRHWIRTGRTVFAEKWGHRDCDIWEIGKEIDKAWEYKKDWWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFA6</accession>
    <name>D5GFA6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00006801001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430208">
        <property type="protein sequence ID" value="CAZ83199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839008.1">
        <property type="nucleotide sequence ID" value="XM_002838962.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFA6"/>
    <dbReference type="FunCoup" id="D5GFA6">
        <property type="interactions" value="74"/>
    </dbReference>
    <dbReference type="GeneID" id="9188055"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006801001"/>
    <dbReference type="eggNOG" id="ENOG502QPTQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007127_10_0_1"/>
    <dbReference type="InParanoid" id="D5GFA6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010961">
        <property type="term" value="P:intracellular magnesium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12829">
        <property type="entry name" value="Alr1p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.340:FF:000008">
        <property type="entry name" value="CorA family metal ion transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.340:FF:000027">
        <property type="entry name" value="CorA family metal ion transporter (Eurofung)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.20">
        <property type="entry name" value="CorA soluble domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.340">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044089">
        <property type="entry name" value="Alr1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045861">
        <property type="entry name" value="CorA_cytoplasmic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045863">
        <property type="entry name" value="CorA_TM1_TM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002523">
        <property type="entry name" value="MgTranspt_CorA/ZnTranspt_ZntB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21535">
        <property type="entry name" value="MAGNESIUM AND COBALT TRANSPORT PROTEIN/MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21535:SF51">
        <property type="entry name" value="MANGANESE RESISTANCE PROTEIN MNR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01544">
        <property type="entry name" value="CorA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143865">
        <property type="entry name" value="CorA soluble domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144083">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="623"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="658"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="81"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ83199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="686" mass="76581" checksum="6E956679C86D55F6" modified="2010-06-15" version="1">MSSSPQHQDSPKDSTTSPGGPSRSRPSHLFAHPNLSNTSIESSALLDHREQQSMRPRRSSTQYSSIPVFSGSRLRMPAGPSNMSATPGPSGSSCRFNSRGFPWEMESEDDGDKEDAPLLGSSMRSAQGADIDPPVSPGKSKRSRPSTAGSMAPPPGRRSANLQPPLAGASRDYGGVNFPPSVPGSPSLGPMPVLGMMGAGDYTNGLGVPSRSLREGDAIINITGDATNDHGYPDSTCPTPLQEFGPGHRQATAEADVCFPVDDGLTDDEVATLHGGSVHAARRRVREWPDLPVLEEWSREEKEERSEGIRAKKTAEPVYVGGRLRAPRKSGWHREVEDEPYRFTYFNDELPATIHSHTISELLQPGQTFKDLFLRIGPRPTFWLDVMNPTDAEMKVFIRAFGIHPLTSEDILMQETREKVELFRSYYLVSYRTFEQDTNSEEYLEPVNIYFVVFREGVISFHFSLTPHPANVRRRIRQLKDYITVSADWISYALIDDITDAFAPLIQSLEEEVDEIDDAILQMHLRSDMLKRVGDTRKKVMGLYRLLGNKADVIKGFAKRCNEQWEVAPRSEIGLYLGDIQDHIVTMVGNLNHMEKILSRSHSNYLAQINIKMNERQEQTADVLGRLTVLGTIVLPMNIITGLWGMNVLVPGQEIDNLNWFWCITAGLLVFGFLCYFIAKRVYKIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFL8</accession>
    <name>D5GFL8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00006979001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430209">
        <property type="protein sequence ID" value="CAZ83311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839120.1">
        <property type="nucleotide sequence ID" value="XM_002839074.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFL8"/>
    <dbReference type="GeneID" id="9188259"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006979001"/>
    <dbReference type="HOGENOM" id="CLU_2851349_0_0_1"/>
    <dbReference type="InParanoid" id="D5GFL8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ83311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="65" mass="7658" checksum="A5F27D014086A62C" modified="2010-06-15" version="1">MCNKGFLRVWTGTVSCLVIPGYHITVRVFMDQYRVFRTAPERLSDLGEQDRLSVPRECRRIAQRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFM8</accession>
    <name>D5GFM8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphoenolpyruvate carboxykinase (ATP)</fullName>
            <ecNumber evidence="3">4.1.1.49</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00006989001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>oxaloacetate + ATP = phosphoenolpyruvate + ADP + CO2</text>
            <dbReference type="Rhea" id="RHEA:18617"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.1.1.49"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the phosphoenolpyruvate carboxykinase (ATP) family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.49" evidence="3"/>
    <dbReference type="EMBL" id="FN430209">
        <property type="protein sequence ID" value="CAZ83321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839130.1">
        <property type="nucleotide sequence ID" value="XM_002839084.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFM8"/>
    <dbReference type="FunCoup" id="D5GFM8">
        <property type="interactions" value="214"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GFM8"/>
    <dbReference type="GeneID" id="9188269"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006989001"/>
    <dbReference type="eggNOG" id="ENOG502QQI5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018247_2_0_1"/>
    <dbReference type="InParanoid" id="D5GFM8"/>
    <dbReference type="OMA" id="MRYAGEM"/>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004612">
        <property type="term" value="F:phosphoenolpyruvate carboxykinase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00484">
        <property type="entry name" value="PEPCK_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.8.10:FF:000001">
        <property type="entry name" value="Phosphoenolpyruvate carboxykinase (ATP)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.449.10:FF:000002">
        <property type="entry name" value="Phosphoenolpyruvate carboxykinase [ATP]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.449.10">
        <property type="entry name" value="Phosphoenolpyruvate Carboxykinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.8.10">
        <property type="entry name" value="Phosphoenolpyruvate Carboxykinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00453">
        <property type="entry name" value="PEPCK_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001272">
        <property type="entry name" value="PEP_carboxykinase_ATP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013035">
        <property type="entry name" value="PEP_carboxykinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008210">
        <property type="entry name" value="PEP_carboxykinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015994">
        <property type="entry name" value="PEPCK_ATP_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00224">
        <property type="entry name" value="pckA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006820">
        <property type="entry name" value="PRK09344.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006821">
        <property type="entry name" value="PRK09344.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30031:SF0">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYKINASE (ATP)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30031">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYKINASE ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01293">
        <property type="entry name" value="PEPCK_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006294">
        <property type="entry name" value="PEP_crbxkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68923">
        <property type="entry name" value="PEP carboxykinase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53795">
        <property type="entry name" value="PEP carboxykinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00532">
        <property type="entry name" value="PEPCK_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="5" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="9" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ83321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="556" mass="62056" checksum="5B806E090D910D33" modified="2010-06-15" version="1">MVSDTVNRTSLHPGGVQPTREHTEIEEELHDKANIDYDRVAIIPNASVAALYEDALVYETGSAITSSGALTAYSGLKTGRSPLDKRIVKEPSSEDEIWWGPVNKPMHPDVWRINRERAVDYLNTRNRIYVVDGYAGWDERYRIKVRVVCARAYHALFMRNMLIRPKPEELVHFEPDYVIYNAGSFPANRYTAGMTSSTSVAINFGEKEMVILGTEYAGEMKKGVFTILFYEMPVKYNVLTLHSSANEGKEGDVTLFFGLSGTGKTTLSADPNRALIGDDEHCWSDRGVFNIEGGCYAKCIGLSAEKEPEIFGSIRFGSVLENVVFNPETRAVDYDDCTLTENTRACYPIEYIQNAKIPCIADSHPSNIILLTCDARAVLPPISKLNNAQTMYHFISGYTSKMAGTEDGVTEPQATFSACFGQPFLALHPMRYARMLSDKITEHKANVWLLNTGWVGASATKGGRRCPLKYTRAILDAIHSGELAQQEYETYETFNLQVPRTAPGVPDQFLNPSKSWSGDATEFQDEVKKLGQLFRENFAKYEDEATQDVKEAAPVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGH1</accession>
    <name>D5GGH1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00007381001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
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                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
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                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
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        <property type="entry name" value="eukaryotic translation initiation factor 2D"/>
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    </evidence>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
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            <taxon>Pezizomycetes</taxon>
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            <taxon>Tuber</taxon>
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        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
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                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="KEGG" id="tml:GSTUM_00007412001"/>
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    <dbReference type="InParanoid" id="D5GGL8"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAZ83640.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="69" mass="7629" checksum="B73F77BA123AB369" modified="2010-06-15" version="1">MLTNERIPVSYFVLLENLEPGSGKLCLSRTTNLLVNRLMRPALPVADPPATPHLPYQRDSKGKKRSVGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGM2</accession>
    <name>D5GGM2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
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    <gene>
        <name evidence="3" type="ORF">GSTUM_00007421001</name>
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    <organism evidence="3 4">
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        <lineage>
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            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
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                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="CAZ83644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002839407.1"/>
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    <dbReference type="KEGG" id="tml:GSTUM_00007421001"/>
    <dbReference type="eggNOG" id="KOG1216">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_305274_0_0_1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000793">
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    </dbReference>
    <dbReference type="GO" id="GO:0000796">
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    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43941">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43941:SF1">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="100"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="765"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="894"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="308"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="378"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="541"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="636"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="840"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="33"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="118"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="770"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="783"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="897"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="914"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="938"/>
            <end position="953"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ83644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="972" mass="109559" checksum="EB68A843F40C62B1" modified="2010-06-15" version="1">MSLSLGLFSRRKNSEDKASKAALCSSHVSTFASSPPSNITTSSTHDGYQPLPLPPHSAPPTHSRKPKLLTKAHPIRVPGHAADSGGIAFAPHLTRARTTNFNSLTPTPQQQQRRYHQKQSSISGLIRSNSDASGSRRVRSLDQRAPQLGSRVEGFVNIDSSNFPHPPSPDLPQCIPLPDSPAAFQNRCGSGSRPSSSTPRLPLAATRVDCRSHSNHGFYNPISEYPISPPLSPESPLRARAGRFNENIHKSEEELVMEIARLKEVITTRDHVIARYAANLNPGEINDVSSGTKGSEGQYANQILSHRIHDLEMANLEKNDEIARAAREMNSLREAHALEVQMLRNAAKRLEAPDGVGLEDLEDLDRYRRREFKLMDVVNGKEKELELLRSNANTLKDQVSVLEKSAAERDRELESARRQIQVMNEDRILIGGKPALVAISEMGLADIGRRLMDEIKRRERAERKLAESAMKSRSDHDAVLLGDRKREETRTNAWREMEHKLTQEELKSHETRRKAKENIDYVLAEEEKRRAELGRQFNERLETLRQEKEVFEGRITNMEVELEQAKQSVTTQREGNESLVQMITKINTRLGEEKKRNKQLEAKIDQITEDALNNVSGSKRACQDLKNVVEDRDTRIAYLESEVTVLKREKSALEEAWHLVESELERHSATLAKRDERIKDLETENVNILDRLEECQHDLEEARKNSPDRPVLGDSDSVTKRELDNLQQDLELYKQDVKAYRHDVKKRDAQIRGLNRTVADLESLLDRKPPPLSPFPPPPPAVSTPTSRPSTPTTANLLEEIATLKDKLRIYDQEYRGTYEDRESLRKKHATVTAQQNMIITDLDSTILRLRKEKESTEKAMKRMQANFLQLSQAATVGTLAVIKKYQEVPLPPLPAGSPSAPGSPIVPSSPVRRGSRRKDKRRSASTGSPVIGSMSGGEEKEKEKEQERRDRALSVVNEPDGALGEEEVIEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGU8</accession>
    <name>D5GGU8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00007520001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430275">
        <property type="protein sequence ID" value="CAZ83720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839529.1">
        <property type="nucleotide sequence ID" value="XM_002839483.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGU8"/>
    <dbReference type="STRING" id="656061.D5GGU8"/>
    <dbReference type="GeneID" id="9181690"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007520001"/>
    <dbReference type="eggNOG" id="ENOG502RXKD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048316_2_0_1"/>
    <dbReference type="InParanoid" id="D5GGU8"/>
    <dbReference type="OMA" id="IWYATAN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070072">
        <property type="term" value="P:vacuolar proton-transporting V-type ATPase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021013">
        <property type="entry name" value="ATPase_Vma12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31394">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 199"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31394:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 199"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11712">
        <property type="entry name" value="Vma12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="125"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ83720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="216" mass="24576" checksum="0433BF01182244FC" modified="2010-06-15" version="1">MVLLTLTSQLTAAIEVYNKENPSSAISLPTTHLDHRQIRIISQTLLNTSEDKSQYRLSTLLQGCNLYHPPPPPKPEPTQEYKYLMARLRQQEEERIYNALTNKPQDIYEEEDEYTFQDLKSQLSIIANVLLSTIATSVAVWMVASGWDVPQRFALAFASSIVVCVAEVVVFNGYLRKVDKSRRLEKIKAQNEVKEVVNVWEIGGVARALDGVKKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGW0</accession>
    <name>D5GGW0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00002061001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430283">
        <property type="protein sequence ID" value="CAZ83753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839562.1">
        <property type="nucleotide sequence ID" value="XM_002839516.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGW0"/>
    <dbReference type="GeneID" id="9181725"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002061001"/>
    <dbReference type="HOGENOM" id="CLU_2442477_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGW0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ83753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="90" mass="10207" checksum="AC9024D8A36FEAF7" modified="2010-06-15" version="1">MKFRRGDFWLGYPSAVRVFSHNSRILLAAPQNALGITFFHLQVGRGGGKRTTLCRAWSFEGCLPFVELEQRDLTDLFPLFWVVTQGGPYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGZ6</accession>
    <name>D5GGZ6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007632001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430297">
        <property type="protein sequence ID" value="CAZ83789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839598.1">
        <property type="nucleotide sequence ID" value="XM_002839552.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGZ6"/>
    <dbReference type="GeneID" id="9185043"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007632001"/>
    <dbReference type="eggNOG" id="ENOG502SBR7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1526277_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGZ6"/>
    <dbReference type="OMA" id="CSKKDSH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000492">
        <property type="term" value="P:box C/D snoRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027921">
        <property type="entry name" value="NOPCHAP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38489">
        <property type="entry name" value="HISTONE CHAPERONE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38489:SF1">
        <property type="entry name" value="HISTONE CHAPERONE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15370">
        <property type="entry name" value="NOPCHAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="153"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="176" mass="19301" checksum="5B71AFBAC818F254" modified="2010-06-15" version="1">MPSDQEQEKERKSNPPHPLITEQSEHAQSPPPPKKPRMAAVDTPSDLMSRLDAFLPAMEAANKDLQAEVEDGTIGKRNIEVLDVEEERYIEMNLGLGVLEGKRDGSVSEPGGSGSGESEGEEGDEEDHDVLLRLLNSSKRHGKGPRGKPSIEVVEHSKDTPTPEKQMDHHVGTENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5GH08</accession>
    <name>D5GH08_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">guanosine-diphosphatase</fullName>
            <ecNumber evidence="5">3.6.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00007646001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">After transfer of sugars to endogenous macromolecular acceptors, the enzyme converts nucleoside diphosphates to nucleoside monophosphates which in turn exit the Golgi lumen in a coupled antiporter reaction, allowing entry of additional nucleotide sugar from the cytosol.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the GDA1/CD39 NTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.42" evidence="5"/>
    <dbReference type="EMBL" id="FN430297">
        <property type="protein sequence ID" value="CAZ83801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839610.1">
        <property type="nucleotide sequence ID" value="XM_002839564.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GH08"/>
    <dbReference type="FunCoup" id="D5GH08">
        <property type="interactions" value="340"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GH08"/>
    <dbReference type="GeneID" id="9185055"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007646001"/>
    <dbReference type="eggNOG" id="KOG1385">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010246_4_0_1"/>
    <dbReference type="InParanoid" id="D5GH08"/>
    <dbReference type="OMA" id="WTCRIKE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004382">
        <property type="term" value="F:GDP phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017111">
        <property type="term" value="F:ribonucleoside triphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045134">
        <property type="term" value="F:UDP phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009134">
        <property type="term" value="P:nucleoside diphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd24040">
        <property type="entry name" value="ASKHA_NBD_GDA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.150">
        <property type="entry name" value="Exopolyphosphatase. Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000407">
        <property type="entry name" value="GDA1_CD39_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11782">
        <property type="entry name" value="ADENOSINE/GUANOSINE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11782:SF83">
        <property type="entry name" value="GUANOSINE-DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01150">
        <property type="entry name" value="GDA1_CD39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01238">
        <property type="entry name" value="GDA1_CD39_NTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="103"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="270"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="301"/>
            <end position="305"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00037742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR600407-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR600407-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ83801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="572" mass="62840" checksum="9C10233707B52C4A" modified="2010-06-15" version="1">MSSARRRPSLWSHFSFQRKPARSSSISSQLRPSDDRYEKSGRHAELNPAFPSMSNRWLKTLSVAVIFALALFILAPKDKSSATSEYVGDELGFLADKTQNISNSRVIPNTNPTTPASSGQGQVALDDESGTKSCTRSSSAKKPIVQYAIMVDAGSTGSRIHVYRFNNCGATPELESEEFKMITPGLSSYKADSEAAAKSLDTLLEVAVEKVPDKLKGCTPIAVKATAGLRLLGDEMSRKTLDAVRQRLETRYLFPVVAGDGVSIMEGKDEGVYAWITTNYLLGKIGGPEDTPTAAIFDLGGGSTQIVFQPTFPSSGGGMPEHLAPGDHKYALNFGGRNFDLYQHSHLGYGLMEARRSIHKLLAETQKKEGDEDWANKPIVNPCIIPGMSREVTVKMGDDEEVKLNMTGPSEPSMPQCRYLAETTLKKGKECKLTPCSFNGVHQPSLARTFAKEDVYIFSYFFDRTRPLGMPDSFTLKEMTETTTRVCGGKDSWDVFQSIDGAMAELLDRPEYCLDLNFMLALLHTGYEMPLDREVKIAKKIKGNELGWCLGASLPLLDQGNGGWQCRIRESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>D5GH90</accession>
    <name>D5GH90_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA ligase</fullName>
            <ecNumber evidence="9">6.5.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00007799001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate.</text>
            <dbReference type="EC" id="6.5.1.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ATP-dependent DNA ligase family.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.1" evidence="9"/>
    <dbReference type="EMBL" id="FN430308">
        <property type="protein sequence ID" value="CAZ83915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839724.1">
        <property type="nucleotide sequence ID" value="XM_002839678.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GH90"/>
    <dbReference type="STRING" id="656061.D5GH90"/>
    <dbReference type="GeneID" id="9182620"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007799001"/>
    <dbReference type="eggNOG" id="KOG0967">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005138_1_1_1"/>
    <dbReference type="InParanoid" id="D5GH90"/>
    <dbReference type="OMA" id="RDFSCEY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003910">
        <property type="term" value="F:DNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006273">
        <property type="term" value="P:lagging strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07900">
        <property type="entry name" value="Adenylation_DNA_ligase_I_Euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07969">
        <property type="entry name" value="OBF_DNA_ligase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000062">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.30:FF:000002">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3260.10">
        <property type="entry name" value="DNA ligase, ATP-dependent, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.30">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050191">
        <property type="entry name" value="ATP-dep_DNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000977">
        <property type="entry name" value="DNA_ligase_ATP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012309">
        <property type="entry name" value="DNA_ligase_ATP-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012310">
        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016059">
        <property type="entry name" value="DNA_ligase_ATP-dep_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012308">
        <property type="entry name" value="DNA_ligase_ATP-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036599">
        <property type="entry name" value="DNA_ligase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00574">
        <property type="entry name" value="dnl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674">
        <property type="entry name" value="DNA LIGASE 1/3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674:SF9">
        <property type="entry name" value="DNA LIGASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04679">
        <property type="entry name" value="DNA_ligase_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01068">
        <property type="entry name" value="DNA_ligase_A_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04675">
        <property type="entry name" value="DNA_ligase_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117018">
        <property type="entry name" value="ATP-dependent DNA ligase DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56091">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00697">
        <property type="entry name" value="DNA_LIGASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50160">
        <property type="entry name" value="DNA_LIGASE_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-dependent DNA ligase family profile" evidence="12">
        <location>
            <begin position="538"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="62"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="596"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="91"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="606"/>
            <end position="621"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ83915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="852" mass="94966" checksum="D2B0280C20029B5D" modified="2010-06-15" version="1">MASPSKKRKNFSNTQPTRTLHHFFNKQTATKKEALDSVLTPDPQLGPVALTDEQYAKRLAEEWKQEDKAIRNATPSTHRESSRAGAKRGRSSSPSTKCAASSPHAHSGEEGWKDNLAAATLRASRAVTPVQKPNTPRILQEPKRIAASAEGALEIERAIDSMPLGEDPLNPEMARRECHLCAPYSRIRPGKFNKVKDKDKDTLVNFLRILIRLDPDSLLPAVWLTTNDIGPSYETNEFGIDGSIISKAIMKASGITPATLKKLNNKHGDPGDVAFEAKVKQRTLMMKKPTPLTIENVYATLVKISRASGKGSQEIKQRHIERLLVDAKGEEIRYLTRTLIRHLRIGAVKTTMLIALSRAFSLSAPKGANWNTPEIPGSKEGRQELFTKAEEFLKQCHARRPNYNDIVPGLLRGGISSLEENCGTSIHSPLKPMLGSITRDLGEMLLKLKGCEFTCEYKYDGQRAQIHCDDNGKVSVFSRHLELMTGKYPDLVQLVPNIRGEGVESFIMEGEVVAMDTETGLVKNFQTLAGRERKNVDVGRISVDVCLFAFDLMYLNGQELLSRPFRERRELLQSIRGYHPGLLPFIVRPKIFTPPASGPPEGAQELESKQIRSRKGKEAAPVKRSSRVKPLLATYEPDKRLESWPKVKKDYTAATDTLDLVPIAAWYGNGRKAAWWSPILLALHDPETGSFEAVCKCMSGFTDKYYIEMKDTLAPDGINTSMTKRAYYEVADSLTPAIWFEPREVWEVAFADITVSPTYTAAGQFMEGRGKGLSLRFPRFVRRREDKGVEEASTPEFLAGLFFKQERDSERISKAAVGGEKKTEGMEQDAEVDGQEFFKNMRGEGAGSDVNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHF0</accession>
    <name>D5GHF0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00007900001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430318">
        <property type="protein sequence ID" value="CAZ83943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839752.1">
        <property type="nucleotide sequence ID" value="XM_002839706.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHF0"/>
    <dbReference type="GeneID" id="9183618"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007900001"/>
    <dbReference type="HOGENOM" id="CLU_1961186_0_0_1"/>
    <dbReference type="InParanoid" id="D5GHF0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ83943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="128" mass="14080" checksum="F1AB7B44DA6CD809" modified="2010-06-15" version="1">MDTLCVAPDRGGIALTGLCCCFSDQRQQATSKMTGYVQQPAVRGGCQDMCEQCFKGKVGADISTLGCYPYRRFGPVPSRYLPDHLRFWKYPAAATKGVNIPHIEGNIARDFVHIVTMLLGREVPILAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHH3</accession>
    <name>D5GHH3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="14">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00007876001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein sumoylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NSE2 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430318">
        <property type="protein sequence ID" value="CAZ83966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839775.1">
        <property type="nucleotide sequence ID" value="XM_002839729.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHH3"/>
    <dbReference type="STRING" id="656061.D5GHH3"/>
    <dbReference type="GeneID" id="9183611"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007876001"/>
    <dbReference type="eggNOG" id="KOG2979">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_787993_0_0_1"/>
    <dbReference type="InParanoid" id="D5GHH3"/>
    <dbReference type="OMA" id="SHWEARN"/>
    <dbReference type="UniPathway" id="UPA00886"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030915">
        <property type="term" value="C:Smc5-Smc6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061665">
        <property type="term" value="F:SUMO ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16651">
        <property type="entry name" value="SPL-RING_NSE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026846">
        <property type="entry name" value="Nse2(Mms21)"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004181">
        <property type="entry name" value="Znf_MIZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21330">
        <property type="entry name" value="E3 SUMO-PROTEIN LIGASE NSE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21330:SF1">
        <property type="entry name" value="E3 SUMO-PROTEIN LIGASE NSE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11789">
        <property type="entry name" value="zf-Nse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51044">
        <property type="entry name" value="ZF_SP_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SP-RING-type" evidence="13">
        <location>
            <begin position="188"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="275"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="67"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="281"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="308"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="335"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ83966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="352" mass="39609" checksum="91B9EC96AC18F0F9" modified="2010-06-15" version="1">MSDRTSHIAIPAYEKPLLPLTAKEIQSLTALINTRSGIAKVSDTIASAITVLGNATLELYPDPEEENEGKEKAMTDMERLARELVDENKRALDMKETLESIVDAQRQALMRGDEPRESSEEQGLSGQYESILVAKKRHWDAMSNTEKYTTSPEYLDFRRSHWEARNPEVPLPPTRTWFSSGASGAQDDESDIEIAQERQSYKCPLTLRPFEDPVRSTVCPHAFEKGAIEDMIKRSRLNGVPCPTPGCTKMLTLRVLKRDSVLKRKVARALALEKRNRQMQEDESEEEEEEASGEEEAQPGPSSRRGKSKAELRKIKIEKGAKKFKGSKKPVAVSSEEEDEEVDADAMDEEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHK2</accession>
    <name>D5GHK2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein disulfide-isomerase</fullName>
            <ecNumber evidence="3">5.3.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00007956001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Catalyzes the rearrangement of -S-S- bonds in proteins.</text>
            <dbReference type="EC" id="5.3.4.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the protein disulfide isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.4.1" evidence="3"/>
    <dbReference type="EMBL" id="FN430319">
        <property type="protein sequence ID" value="CAZ83995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839804.1">
        <property type="nucleotide sequence ID" value="XM_002839758.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHK2"/>
    <dbReference type="STRING" id="656061.D5GHK2"/>
    <dbReference type="GeneID" id="9183649"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007956001"/>
    <dbReference type="eggNOG" id="KOG0191">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038617_1_1_1"/>
    <dbReference type="InParanoid" id="D5GHK2"/>
    <dbReference type="OMA" id="FINEHAG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02998">
        <property type="entry name" value="PDI_a_ERp38"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000032">
        <property type="entry name" value="Protein disulfide-isomerase A6 homolog"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1150.12">
        <property type="entry name" value="Endoplasmic reticulum resident protein 29, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011679">
        <property type="entry name" value="ERp29_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036356">
        <property type="entry name" value="ERp29_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051063">
        <property type="entry name" value="PDI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005788">
        <property type="entry name" value="PDI_thioredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01126">
        <property type="entry name" value="pdi_dom"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45672:SF11">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE C17H9.14C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45672">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE C17H9.14C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07749">
        <property type="entry name" value="ERp29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00421">
        <property type="entry name" value="THIOREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47933">
        <property type="entry name" value="ERP29 C domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072154" description="protein disulfide-isomerase" evidence="10">
        <location>
            <begin position="20"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="11">
        <location>
            <begin position="14"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="11">
        <location>
            <begin position="134"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ83995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="353" mass="38566" checksum="C7923ECD33B4920D" modified="2010-06-15" version="1">MKDFCSLFTLASLLLVATASNVIDLTPDNFDKEILQNGRPALVEFFAPWCGHCKSLAPVYEELADSLASQKDKVAIAKVDADNHKALGKRFGVSGFPTLKWFDGKSADPIPYESGRDLEALQAFLKEKVGGLKLKAKREAPSNVIVLSDANFDKIVHDEKKDVLVEFYAPWCGHCKNLAPIYEKLAKNFASETNVVVAKLDADSPGGKASAEKYGITGFPTLKWFPKGSSAKEPILYESARSEEALTQFINKHAGTHRVVGGGVDDAAGRIASLDTIVQKLISGEKDAEKELAKAAEENADYPKKEIKRLEGIAAKGGLAPEKLDDILSRKNILTQFLSKENEGEREEVKEEL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-07-27" modified="2025-06-18" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHP2</accession>
    <name>AMPP2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName>Probable Xaa-Pro aminopeptidase GSTUM_00008071001</fullName>
            <ecNumber>3.4.11.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Aminoacylproline aminopeptidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Prolidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ORF">GSTUM_00008071001</name>
    </gene>
    <organism>
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>Release of any N-terminal amino acid, including proline, that is linked to proline, even from a dipeptide or tripeptide.</text>
            <dbReference type="EC" id="3.4.11.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M24B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.9"/>
    <dbReference type="EMBL" id="FN430320">
        <property type="protein sequence ID" value="CAZ84072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839881.1">
        <property type="nucleotide sequence ID" value="XM_002839835.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHP2"/>
    <dbReference type="SMR" id="D5GHP2"/>
    <dbReference type="FunCoup" id="D5GHP2">
        <property type="interactions" value="372"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GHP2"/>
    <dbReference type="EnsemblFungi" id="CAZ84072">
        <property type="protein sequence ID" value="CAZ84072"/>
        <property type="gene ID" value="GSTUM_00008071001"/>
    </dbReference>
    <dbReference type="GeneID" id="9183726"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008071001"/>
    <dbReference type="eggNOG" id="KOG2737">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017266_1_2_1"/>
    <dbReference type="InParanoid" id="D5GHP2"/>
    <dbReference type="OMA" id="DAHALFF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01087">
        <property type="entry name" value="Prolidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007865">
        <property type="entry name" value="Aminopep_P_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052433">
        <property type="entry name" value="X-Pro_dipept-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43226">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43226:SF1">
        <property type="entry name" value="XAA-PRO DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05195">
        <property type="entry name" value="AMP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01011">
        <property type="entry name" value="AMP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53092">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0031">Aminopeptidase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0482">Metalloprotease</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000411855" description="Probable Xaa-Pro aminopeptidase GSTUM_00008071001">
        <location>
            <begin position="1"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="386"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="426"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="426"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="455" mass="50793" checksum="BB374E5FB69A7739" modified="2010-06-15" version="1">MTSDSTVDKILAGKYPAKQHAENVVERLLHVHPDLTDGVIYLESQRSKLYENSDQEVPFRQRRYFYYLSGCDLADSYLTYSIRDRKLTLFIPPIDPASVLWSGLPLSNSEALEKYDVDEVLPTSATALPTTSYSSLMFVIESQTSRTFHLQNTESLEPAIERARAIKDEYEVALIKKANRISALAHHSCLRAIKSAGNEREIEAVFTKECIANGAPKQAYSGIFGSGRSASTLHYVHNNQPLAGKLNLLLDAGAEYNNYASDITRTFPISGQFTKESREVYDIVLDMQKQCLAASKAGAVWDDIHILAHKVAIQGLLKIGVLRNGSVDEILSNRTSTAFLPHGLGHYLGMDTHDCGGNPNYADPDPMFKYLRKRGPLPAGAVITVEPGIYFCEFIIKPYLEDEKHAKYIDKDVLNRYWDVGGVRIEDNILITEGGYENLTNVAKEVDDMLKFING</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHZ2</accession>
    <name>D5GHZ2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chromatin modification-related protein EAF3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Chromatin modification-related protein eaf3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">GSTUM_00008199001</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Involved in deacetylation of histones, chromatin assembly and chromosome segregation. May act as a transcriptional oscillator, directing histone deacetylases to specific chromosomal domains. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of selected genes principally by acetylation of nucleosomal histone H4 and H2A. The NuA4 complex is also involved in DNA repair.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the NuA4 histone acetyltransferase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MRG family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430322">
        <property type="protein sequence ID" value="CAZ84135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839944.1">
        <property type="nucleotide sequence ID" value="XM_002839898.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHZ2"/>
    <dbReference type="FunCoup" id="D5GHZ2">
        <property type="interactions" value="618"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GHZ2"/>
    <dbReference type="GeneID" id="9184228"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008199001"/>
    <dbReference type="eggNOG" id="KOG3001">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039566_1_1_1"/>
    <dbReference type="InParanoid" id="D5GHZ2"/>
    <dbReference type="OMA" id="GLQTYFD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032221">
        <property type="term" value="C:Rpd3S complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18983">
        <property type="entry name" value="CBD_MSL3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.274.30:FF:000004">
        <property type="entry name" value="Putative Chromatin modification-related protein eaf3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.30">
        <property type="entry name" value="MRG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008676">
        <property type="entry name" value="MRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038217">
        <property type="entry name" value="MRG_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026541">
        <property type="entry name" value="MRG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053820">
        <property type="entry name" value="MSL3_chromo-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10880">
        <property type="entry name" value="MORTALITY FACTOR 4-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10880:SF15">
        <property type="entry name" value="MSL COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05712">
        <property type="entry name" value="MRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22732">
        <property type="entry name" value="MSL3_chromo-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038133">
        <property type="entry name" value="HAT_Nua4_EAF3/MRG15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51640">
        <property type="entry name" value="MRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Chromo" evidence="14">
        <location>
            <begin position="26"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="78"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="83"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00057322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00072864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAZ84135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="300" mass="34897" checksum="03A1EB428242AF32" modified="2010-06-15" version="1">MPPAASQKVLTYEVQERVLCFHGPMLYEAKVLEVKKSEDKKELPEYKVHYKGWKNTWDEFVPADRLRKMNEENLRLQKELNDNTKPSSKRTNPTPNAKAGMKMSKAGSEDLPGASGTLPPRGQKRGRELEIEKEEDFMKRHDIKITIPDNLKALLVDDWENVTKNQQLVPLPRNPSVTQILQKYRESMPKKREGSADMDIFEEVLAGLKLYFDKSLGTILLYRFERQQYMEIRKEHPGKEPSELYGAEHLLRLFVSMPELLAHTNMDPQSVSKLREHIEDFIRFLGKNPEQYVAEHYESG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>D5GI29</accession>
    <name>D5GI29_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00008244001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CENP-O/MCM21 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430322">
        <property type="protein sequence ID" value="CAZ84172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839981.1">
        <property type="nucleotide sequence ID" value="XM_002839935.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GI29"/>
    <dbReference type="STRING" id="656061.D5GI29"/>
    <dbReference type="GeneID" id="9183505"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008244001"/>
    <dbReference type="eggNOG" id="ENOG502SCC4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_767210_0_0_1"/>
    <dbReference type="InParanoid" id="D5GI29"/>
    <dbReference type="OMA" id="RIESVEW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031511">
        <property type="term" value="C:Mis6-Sim4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd23830">
        <property type="entry name" value="DRWD-N_Mcm21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018464">
        <property type="entry name" value="CENP-O"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14582:SF1">
        <property type="entry name" value="CENTROMERE PROTEIN O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14582">
        <property type="entry name" value="INNER KINETOCHORE SUBUNIT MAL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09496">
        <property type="entry name" value="CENP-O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0137">Centromere</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="55"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ84172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="318" mass="35649" checksum="21EC0383372FE891" modified="2010-06-15" version="1">MPSQSNELSSVEDFDLLSIEARLTELERHKSLLTAELLSYRSTLPISTILSQAKVRPSYEDPTSVTSTVGPGTETSSNASGASDGKSEANDEDILSDVIAHAKCSQKERLKKLYRMTGITTFTSEDPSPPHNVNVGIRFEIFGNRKFTRRQYIILSLASNTYTILRHSIPSFLHVHSLRAQYPHDLAKFARVLRAKLQLFARRLDAVEKLEQTLTNMGRIGDVQEPWTNRIESVEWDVPVRMIVINWSIGVRGSLVLDERGSIEAVVVRDNDGERMKKLEAVIRSGNLDGVAVRLGYVMDREEIYEEDGDWEAGVATS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIC4</accession>
    <name>D5GIC4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ATP synthase subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00008424001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="11">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) and CF(0) have multiple subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the ATPase gamma chain family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430326">
        <property type="protein sequence ID" value="CAZ84267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840076.1">
        <property type="nucleotide sequence ID" value="XM_002840030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIC4"/>
    <dbReference type="FunCoup" id="D5GIC4">
        <property type="interactions" value="866"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GIC4"/>
    <dbReference type="GeneID" id="9184625"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008424001"/>
    <dbReference type="eggNOG" id="KOG1531">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050669_4_1_1"/>
    <dbReference type="InParanoid" id="D5GIC4"/>
    <dbReference type="OMA" id="MQITSAM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12151">
        <property type="entry name" value="F1-ATPase_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.80:FF:000001">
        <property type="entry name" value="ATP synthase gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1380.10:FF:000003">
        <property type="entry name" value="ATP synthase subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1380.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.80">
        <property type="entry name" value="ATP synthase, gamma subunit, helix hairpin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035968">
        <property type="entry name" value="ATP_synth_F1_ATPase_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000131">
        <property type="entry name" value="ATP_synth_F1_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023632">
        <property type="entry name" value="ATP_synth_F1_gsu_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01146">
        <property type="entry name" value="ATPsyn_F1gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11693">
        <property type="entry name" value="ATP SYNTHASE GAMMA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11693:SF22">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT GAMMA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00231">
        <property type="entry name" value="ATP-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039089">
        <property type="entry name" value="ATP_synthase_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00126">
        <property type="entry name" value="ATPASEGAMMA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52943">
        <property type="entry name" value="ATP synthase (F1-ATPase), gamma subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00153">
        <property type="entry name" value="ATPASE_GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="9 11" id="KW-0139">CF(1)</keyword>
    <keyword evidence="4 11" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="6 11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ84267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="295" mass="32469" checksum="BB15686750E53CA2" modified="2010-06-15" version="1">MFYRAARPALQRVAAASTRPAQSNNYATLREIEGRLKSIKNIEKITNTMKIVASTKLARAQRAMSESRTYGQTSNTVFDKAETKPQEGGKTLIIACSSDKGLCGGIHSQITKAVRRKLLETPDADLVILGEKSKAQLGRSSAKNIVMSFANVGKDIPTFAEAQTIADQIFQLEKDYDNIEVIYNNFKSAISYEPGSVYAFSEDIIKSSANFTAFEIEDEQLVNLREYALANTIFWALSEGHACEISARRNAMDNASKNAGDMINRFQILFNRTRQAVITGELVEIITGAAASEDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIC6</accession>
    <name>D5GIC6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00008426001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430326">
        <property type="protein sequence ID" value="CAZ84269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840078.1">
        <property type="nucleotide sequence ID" value="XM_002840032.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIC6"/>
    <dbReference type="STRING" id="656061.D5GIC6"/>
    <dbReference type="GeneID" id="9184627"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008426001"/>
    <dbReference type="eggNOG" id="KOG1430">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045030_1_0_1"/>
    <dbReference type="InParanoid" id="D5GIC6"/>
    <dbReference type="OMA" id="PQTAWLN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050177">
        <property type="entry name" value="Lipid_A_modif_metabolic_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43245">
        <property type="entry name" value="BIFUNCTIONAL POLYMYXIN RESISTANCE PROTEIN ARNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43245:SF11">
        <property type="entry name" value="LD23561P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="2">
        <location>
            <begin position="18"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="372"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="373"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ84269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="392" mass="44203" checksum="516A5D90C8D84674" modified="2010-06-15" version="1">MTEVTAPAPTGTDTKPSVLIIGGLGFIGRFLAKYIHTNNLASEIRIVDKQLPQLAWLAPEFTDVCTLERFQQGDLSREHTCERVFNRPDGSTWDYVFNCGGDTRYSQDDEIYKQRSLKLSTTAAKEAARKGVKCWVEISTGAVYKSDREPSKETDKLKPWLKLAKYKLQAEDELKQIEGLNLVVVRLANVYGEYCSKVIATILCMARVYAYLKEEMKWLWTKDLRTHTVHVTDKNWDESMGSTPIFNVVDHGDTSQGTLQKHISNIFGIKTGFHGTIVSSFARLNLGSAVDEENDEILQPWGDLLNEAGITRPGPINPYLDNELVKDDDLSLDGTRFEKVTGFGYRMPEITEEGLREVIDSYRRLNWWPPMEPGNGENGENGQAVVAEAARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIE4</accession>
    <name>D5GIE4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Probable beta-glucosidase btgE</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Beta-D-glucoside glucohydrolase btgE</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Cellobiase btgE</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Gentiobiase btgE</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">GSTUM_00008448001</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Beta-glucosidases are one of a number of cellulolytic enzymes involved in the degradation of cellulosic biomass. Catalyzes the last step releasing glucose from the inhibitory cellobiose.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430326">
        <property type="protein sequence ID" value="CAZ84287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840096.1">
        <property type="nucleotide sequence ID" value="XM_002840050.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIE4"/>
    <dbReference type="STRING" id="656061.D5GIE4"/>
    <dbReference type="GeneID" id="9184645"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008448001"/>
    <dbReference type="eggNOG" id="ENOG502QS0R">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027285_2_0_1"/>
    <dbReference type="InParanoid" id="D5GIE4"/>
    <dbReference type="OMA" id="VVCPYAT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050732">
        <property type="entry name" value="Beta-glucan_modifiers"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16631:SF24">
        <property type="entry name" value="FAMILY 17 GLUCOSIDASE SCW11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16631">
        <property type="entry name" value="GLUCAN 1,3-BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0134">Cell wall</keyword>
    <keyword evidence="7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072807" description="Probable beta-glucosidase btgE" evidence="14">
        <location>
            <begin position="21"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="290"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00042762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAZ84287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="583" mass="59228" checksum="5F1A34F72194D9CF" modified="2010-06-15" version="1">MRYSLFAVAATALLSGSAAARGHQHRRHAHPHALAPRGECEVSPPQCKTYTSSILVPVGTVPPSDASTVKQTMTVVPVVEPTTTIKTTVTYTSTSTNVVTVTKPKSASVPSASSAPVVPTPIVTVCPTPGTYTIPAQTVVLTSTETVCVPETTSLPPGTHTIGGVTTVVHTATTVVCPVAAVETASGVTTSKVVLTTYVCPTAGTYTIGASTTTVTGTVTVPCTYPVPTAYPPGTYTHPEVVTTVTVTSAVVVCPYTTKGLPTSAPGAHTTTPLPPASTVSATSVASSVAPTTTPLAPSSTASATPVSSSSAPSSSATSKPVSGGGLWAITYSPYTANGGCKTASEVSADITSIASKGFQNIRLYSTDCDGLKNVGSACEANGVGIILGVFIKAGGVSTADEQVKDILEWKKFELVVLFVVGNEAVFNGFCTAEELAAYISKVKGQLQGAGYTGHVTTAETLNILQAHGSVLCSAMDVVGVNAHAFFNPQNTAEKAGEFVKGQLKLASDACGGKPGWVLEAGWPSAGQPNGNAVPGAAEQKAAIASMVKECPGQITYFSFENDLWKTAGAFGVEPNFGCLGVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIL1</accession>
    <name>D5GIL1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008546001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430327">
        <property type="protein sequence ID" value="CAZ84354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840163.1">
        <property type="nucleotide sequence ID" value="XM_002840117.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIL1"/>
    <dbReference type="STRING" id="656061.D5GIL1"/>
    <dbReference type="GeneID" id="9183288"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008546001"/>
    <dbReference type="eggNOG" id="KOG2142">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010913_2_0_1"/>
    <dbReference type="InParanoid" id="D5GIL1"/>
    <dbReference type="OMA" id="AANEHRI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008265">
        <property type="term" value="F:molybdenum cofactor sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043545">
        <property type="term" value="P:molybdopterin cofactor metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14237:SF80">
        <property type="entry name" value="MOLYBDENUM COFACTOR SULFURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14237">
        <property type="entry name" value="MOLYBDOPTERIN COFACTOR SULFURASE MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="1">
        <location>
            <begin position="43"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Aminotransferase class V" evidence="1">
        <location>
            <begin position="197"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="556" mass="61904" checksum="E9C4E59BC45B267C" modified="2010-06-15" version="1">MSPSFRSRASKRGLRARYELNPGYNPNIEKLRIKEYPQLKDQTYLDHSGTTLYAESLLTTISADLRENLYGNPHSENPSSKLSARKVTAVRLKVLALFKADPAKYDVIFCANTTAGVKLVADSFTGHGQDGGKGKGNFKYRFHKDCHTSLIGPRGMAGSAECFWGDEEVEAWLDSPEEDEEEGEEEGSLGLFAWPGQSNFSGRRLPIHAWSSKLRETRGGRYYSLFDAAALVMTSPLDLSDADASPDFIVCSFYKMFGYPDLGALIVRRESAGVLQHRRYFGGGTVGQLMASMDHVKRMYHKDPHEHLEDGTCAFHSIIALGHAIDTHKRLYESFERISAHTASLAAMLAGMLAELTHRSTGRKLVEIYTEEKYGDPKRQGPVVTFNLCDSRGEPISYLAVEAAASARNIHVRSGGHCNPGGVQQYCRLSKEKMMSFFHSGKMCGDNRDVIDGKHVGALRASLGAMSTLEDVVTFVDFVREEYVSKPLSLEEKRRILLQERKKDVEEVKRVMKKYGTVFSSSRTGKTLLCFWTDSVNLDEALPMNTRLASNSLSVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIN7</accession>
    <name>D5GIN7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="18">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">GSTUM_00008588001</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. KIN-5/BimC subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430327">
        <property type="protein sequence ID" value="CAZ84380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840189.1">
        <property type="nucleotide sequence ID" value="XM_002840143.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIN7"/>
    <dbReference type="FunCoup" id="D5GIN7">
        <property type="interactions" value="978"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GIN7"/>
    <dbReference type="GeneID" id="9183314"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008588001"/>
    <dbReference type="eggNOG" id="KOG0243">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001485_33_2_1"/>
    <dbReference type="InParanoid" id="D5GIN7"/>
    <dbReference type="OMA" id="DWETFDQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="term" value="C:mitotic spindle"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:nucleus"/>
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        <property type="term" value="C:spindle microtubule"/>
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        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="F:microtubule binding"/>
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        <property type="term" value="F:plus-end-directed microtubule motor activity"/>
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        <property type="term" value="P:cell division"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:initial mitotic spindle pole body separation"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="CDD" id="cd01364">
        <property type="entry name" value="KISc_BimC_Eg5"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Kinesin-like protein bimC"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Kinesin_motor_dom"/>
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        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="entry name" value="KINESIN FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="KINESIN-LIKE PROTEIN KIF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Microtub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="9 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7" id="KW-0498">Mitosis</keyword>
    <keyword evidence="10 14" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="17">
        <location>
            <begin position="98"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1015"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1070"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="493"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="677"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="753"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="49"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="185"/>
            <end position="192"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="18">
        <location>
            <position position="1100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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            <dbReference type="ARBA" id="ARBA00022553"/>
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            <dbReference type="ARBA" id="ARBA00022618"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00034704"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAZ84380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1100" mass="122500" checksum="7B1510333B3BEBD6" modified="2010-06-15" version="1" fragment="single">MSGPSRFQNGGVRKTNASSNTMRAPVTARPSSRNGMAHPQSYMTAARMGAGALPSSPAGSVASVSSRARGNASPTKRRIKKDLAVEEPPPQQEDNEANINVVVRCRGRSEREIKENSGVVVSTPGGLRGKEISLSMGPLALSNKTYTFDRVFGPEANQNMIYDNVVAPILEEMLSGYNCTIFAYGQTGTGKTYTMSGDMTDNFGTYSDSAGIIPRALYQLFHKLGIDEADNSVKCSFIELYNEELKDLLALDENNKVKIFEDSTRKGIVIQGMEESFIKNAEDGVKLLQEGSHKRQVAATKCNDLSSRSHTVFTITVHVKEVGEDGEDLLRTGKLNLVDLAGSENIGRSGAENKRAREAGMINQSLLTLGRVINALVDKSPHIPYRESKLTRLLQDSLGGRTKTCIIATVSPAKSNLEETISTLDYAARAKNIRNKPQINQMLTKKALIREYVTEIERLKGDLMATRQKNGVFLTTDSYSEMTEESESRRILNEEQQRKIDVMETQIKNTREQFEQNMRLFLELKKELEGTKGVLEVTKGELRKTEVDLSSTRKDLADEAVLREAHEKTEEELRDVGQKLITRLDETVHDVSGLHAKIRRMTDLEVVNHSSWMKSSGQVSTITELVEKEISTFTERQERITDHVSERMSTFVATEVKRLESAYKHIEARLGGFSNGEAELSAETMKAKDEMNQVLEEIKILREDVKQKVGEGLKGLNEAARRISAEVVEDLGKFGIQLHHSYNQLGREFKAIFDDVEKQIVAQKAEAEKLRLQLSSATAAVVIATDTAQSSLDSILREERERAATDRQNLIGQITALINATAEDQDKRLTKRVQLVQGEILGAKEGLDEATKQYNAGMDQWSGVEEKFINELVISQDSLKEILAHDWQDAEKHNASIQATTQAINAQTVRLVDAQMQDVGVQMQALDEFVTRARSQNEAHFEAFTQNLSGLSETVRESYRRMEGDLNGFTEDLDSFSNDMSEQTSLSKVLLEPFTSTTKQPLSELRENIETAPLQGYVPTGETPKRRTYEYPTTLPHTQPHEELLLEPVTRRKSSIRTPLGEKEAFLANLQRPVTPPTPTKSSSEQISQHILASPLEVVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIP7</accession>
    <name>D5GIP7_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS10m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">37S ribosomal protein S10, mitochondrial</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Mitochondrial ribosomal small subunit protein 10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00008599001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS10 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430328">
        <property type="protein sequence ID" value="CAZ84390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840199.1">
        <property type="nucleotide sequence ID" value="XM_002840153.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIP7"/>
    <dbReference type="STRING" id="656061.D5GIP7"/>
    <dbReference type="GeneID" id="9183324"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008599001"/>
    <dbReference type="eggNOG" id="KOG3321">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_514073_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIP7"/>
    <dbReference type="OMA" id="YIHEHEM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.600:FF:000003">
        <property type="entry name" value="30S ribosomal protein S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.600">
        <property type="entry name" value="Ribosomal protein S10 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00508">
        <property type="entry name" value="Ribosomal_uS10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001848">
        <property type="entry name" value="Ribosomal_uS10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027486">
        <property type="entry name" value="Ribosomal_uS10_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036838">
        <property type="entry name" value="Ribosomal_uS10_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01049">
        <property type="entry name" value="rpsJ_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11700">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S10 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00338">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01403">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54999">
        <property type="entry name" value="Ribosomal protein S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS10" evidence="9">
        <location>
            <begin position="89"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="439"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="507"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="272"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="478"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00042916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00078476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ84390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="530" mass="57805" checksum="9AE1CC1C4A0EBCCA" modified="2010-06-15" version="1">MFAFRSRSAVTRVRTIRRPLGVCSVLPRSNFSTAALLRQEITEETSASNPGAGAADPDADPEPRIPLSIQSVWYRPFKRKAEHGLPVCNLQIRSYNVRNLEFMCDFALRAAYYLNLPASGPVPLPRKTERWTVPRGHFVHKKSQENFERVTYKRLIQIKDGHPETVEIWLAYLKKHQFYGTGMKANVWGFEELGVGKRMNVSLDNLRDVAAKPKWAHSGVGKTLETTERAAEILNSPEYKALANATDVEEAEREVLQAAPLETIEGLEGALEAEVEETIQKVEEEAMKAEESREEAEVNAVYQMVDETAELEEPTELKAQPIGEVKAETQTDPLGPSSAIEGVPVTTTSPVADVAEQLPVAQAQEEVCILAASTIETMPSEVKEVPDLPKDPSADQAEEVVAIITPSGTLAAATALVADTPKETAPEEPLAETIRATPLPLETSATDPVVETPATTVEEPLGPPETAALEEEFRPETESQGDTAQTAPEVTKVQKAEVGVEIEVEKAAKETAANETTNVDAVEPKTDKMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIR8</accession>
    <name>D5GIR8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008633001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430328">
        <property type="protein sequence ID" value="CAZ84411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840220.1">
        <property type="nucleotide sequence ID" value="XM_002840174.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIR8"/>
    <dbReference type="GeneID" id="9185629"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008633001"/>
    <dbReference type="HOGENOM" id="CLU_950587_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIR8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="213"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="255"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="49"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="232"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="293" mass="33466" checksum="54D6AF61F3B6BFED" modified="2010-06-15" version="1">MTSVQGIYPQPYLFPRDFEDIMDMFPPSPEDPELVPILDREDNSDLSRPDSSISSYSSRSSTDSLCIPEISFRYTGMQTPQKHQLPPPPPPTPFLTSPLMSPLEHGLEDDRDLYSSPFVLSANLLELGETLGDLFVESDYEYREMGESPYVPFQHKPKHLSDSWSLPCSFKDLADYPALNAEPYGNEGRYLPPIRSSFDEGLFMAREAPYVRHTNRTKKSISRTPPPSRHSGGSEERPWSLSEIEKQLQILADERARAEELEEQGELTERRISRRGGPMKKISRAWKGYLHRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJG4</accession>
    <name>D5GJG4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Elongation of fatty acids protein</fullName>
            <ecNumber evidence="10">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00008988001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>an acyl-CoA + malonyl-CoA + H(+) = a 3-oxoacyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:50252"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:90726"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:50253"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the ELO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="10"/>
    <dbReference type="EMBL" id="FN430330">
        <property type="protein sequence ID" value="CAZ84657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840466.1">
        <property type="nucleotide sequence ID" value="XM_002840420.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJG4"/>
    <dbReference type="STRING" id="656061.D5GJG4"/>
    <dbReference type="GeneID" id="9182658"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008988001"/>
    <dbReference type="eggNOG" id="KOG3072">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017661_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJG4"/>
    <dbReference type="OMA" id="WNQGLAY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009922">
        <property type="term" value="F:fatty acid elongase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034625">
        <property type="term" value="P:fatty acid elongation, monounsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034626">
        <property type="term" value="P:fatty acid elongation, polyunsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019367">
        <property type="term" value="P:fatty acid elongation, saturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002076">
        <property type="entry name" value="ELO_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157:SF169">
        <property type="entry name" value="ELONGATION OF FATTY ACIDS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157">
        <property type="entry name" value="FATTY ACID ACYL TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01151">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5 10" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="215"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="256"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="321"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ84657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="367" mass="40126" checksum="CE4CE4829F05BFE8" modified="2010-06-15" version="1">MQAAVESFIPSLKVAFPPSSWLALLPSPSTASPTIPPSASKYPFTISESLYTAFLDPRVPLSIAAAYAAAVHLLNSRCNGKPYCIATTRIFKAFVLLHNLLLAVYSLWTFIGMAGAIHRSIVTSRGVPGVVESLCKIRPETRFEGVEIREMEGGVGLWEEGLAFYGWLFYLSKFYEVLDTAIIIAKGKKSSLLQTYHHAGAMICMWAGIRFMSPPIWVFCIFNSLIHTLMYFYYTLSTVRIPVPNALKRSLTSLQITQFVVGGSLAALHLFVYFTPTTYLPGSVTEVVPSSSASASVSRHGMPVDVKAPGMVHCLSNPGEVWAVVANVVYLTPLTYLFVSFFVESYTKGGKTKRAAKAAAPTEKPAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJX7</accession>
    <name>D5GJX7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00009260001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430334">
        <property type="protein sequence ID" value="CAZ84820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840629.1">
        <property type="nucleotide sequence ID" value="XM_002840583.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJX7"/>
    <dbReference type="STRING" id="656061.D5GJX7"/>
    <dbReference type="GeneID" id="9186856"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009260001"/>
    <dbReference type="eggNOG" id="KOG2428">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_512834_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJX7"/>
    <dbReference type="OMA" id="TNIYRWS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016593">
        <property type="term" value="C:Cdc73/Paf1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990269">
        <property type="term" value="F:RNA polymerase II C-terminal domain phosphoserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007149">
        <property type="entry name" value="Leo1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23146">
        <property type="entry name" value="LEO1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23146:SF0">
        <property type="entry name" value="RNA POLYMERASE-ASSOCIATED PROTEIN LEO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04004">
        <property type="entry name" value="Leo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="286"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="303"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="350"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="381"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="411"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="427"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="462"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="471" mass="52638" checksum="DB1A948313697C11" modified="2010-06-15" version="1">MSSPGSVLSDIEASPLAASPERPTGNKYDRLFGSDNEGAVARDEEAEASDDEDIGRKRRSRVTDNLDGVNDEEEEEEDGDRGLFGDDDDDEPEKKDLSDVESHASHDPGNDEQQTFHSIDVSLPRHACPDQGNDDLYLMRIPSTVNFQQQIFYPENFTMPKATEDSTVSPYTMATTTIRVRKSPANPSVLQSNARVVKWSDGSLSLQIASSPNLYDLTSKPLAPPQNSKAYDAAQDSHTYLLTTHESAGMLRFVGHVNTHLNLLPTSTSQANAEAVNRLQERLYQAQSTRGARGRGAQALEISDMRDPEAERKEAEKQHRDKEKLAKKAEAKKARASRNGAGADEDSRAAARRSYPRNSHTTRSKRDSPPIGGKGGRSKEDEYDLEDGFLEKSEDDEEDEMEESEDEGDRRRGHGKKRAVKKRIPSPEEEEEEEEEEEAEFTDEERAPSRTVRAGKRRRVIDDDEEDDDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GK81</accession>
    <name>D5GK81_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00009419001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Has a physiological role leading to 18S rRNA stability.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the small ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS21 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430337">
        <property type="protein sequence ID" value="CAZ84924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840733.1">
        <property type="nucleotide sequence ID" value="XM_002840687.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GK81"/>
    <dbReference type="FunCoup" id="D5GK81">
        <property type="interactions" value="862"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GK81"/>
    <dbReference type="GeneID" id="9186953"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009419001"/>
    <dbReference type="eggNOG" id="KOG3486">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_167122_2_0_1"/>
    <dbReference type="InParanoid" id="D5GK81"/>
    <dbReference type="OMA" id="GESDACM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022626">
        <property type="term" value="C:cytosolic ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1230.20:FF:000001">
        <property type="entry name" value="40S ribosomal protein S21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1230.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001931">
        <property type="entry name" value="Ribosomal_eS21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018279">
        <property type="entry name" value="Ribosomal_eS21_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038579">
        <property type="entry name" value="Ribosomal_eS21_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10442">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01249">
        <property type="entry name" value="Ribosomal_S21e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002148">
        <property type="entry name" value="Ribosomal_S21e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00996">
        <property type="entry name" value="RIBOSOMAL_S21E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR002148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ84924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="92" mass="10244" checksum="02FCFBD1713905AA" modified="2010-06-15" version="1">MSLLTQYTGEVVDLYVPRKCSATNRIIKAKDHASVQISVGKVDESGRYIHGENITYALSGFVRSMGESDDALNRLAQRDGLLKNVWSASRQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKT1</accession>
    <name>D5GKT1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00009749001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="FN430341">
        <property type="protein sequence ID" value="CAZ85124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840933.1">
        <property type="nucleotide sequence ID" value="XM_002840887.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKT1"/>
    <dbReference type="STRING" id="656061.D5GKT1"/>
    <dbReference type="GeneID" id="9183360"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009749001"/>
    <dbReference type="eggNOG" id="KOG1509">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2672901_0_0_1"/>
    <dbReference type="InParanoid" id="D5GKT1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047429">
        <property type="term" value="F:nucleoside triphosphate diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003697">
        <property type="entry name" value="Maf-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43213">
        <property type="entry name" value="BIFUNCTIONAL DTTP/UTP PYROPHOSPHATASE/METHYLTRANSFERASE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43213:SF5">
        <property type="entry name" value="BIFUNCTIONAL DTTP_UTP PYROPHOSPHATASE_METHYLTRANSFERASE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02545">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="ITPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ85124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="75" mass="7985" checksum="B9B327D8BACB15AD" modified="2010-06-15" version="1">MVTDDLLEAYVKTREGADKAGGYAIQGAGSILVKKIEGTFDNVVGLPLRGTLKVIEKVLSNDEMVEEGEDEGGED</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLA8</accession>
    <name>D5GLA8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="11">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00010106001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Nudix hydrolase family. DCP2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430348">
        <property type="protein sequence ID" value="CAZ85301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841110.1">
        <property type="nucleotide sequence ID" value="XM_002841064.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLA8"/>
    <dbReference type="SMR" id="D5GLA8"/>
    <dbReference type="STRING" id="656061.D5GLA8"/>
    <dbReference type="GeneID" id="9182095"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010106001"/>
    <dbReference type="eggNOG" id="KOG2937">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008108_4_0_1"/>
    <dbReference type="InParanoid" id="D5GLA8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140933">
        <property type="term" value="F:5'-(N(7)-methylguanosine 5'-triphospho)-[mRNA] hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03672">
        <property type="entry name" value="NUDIX_Dcp2p_Nudt20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000003">
        <property type="entry name" value="M7GpppN-mRNA hydrolase isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1050">
        <property type="entry name" value="Dcp2, box A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007722">
        <property type="entry name" value="DCP2_BoxA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036189">
        <property type="entry name" value="DCP2_BoxA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044099">
        <property type="entry name" value="Dcp2_NUDIX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23114">
        <property type="entry name" value="M7GPPPN-MRNA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23114:SF17">
        <property type="entry name" value="M7GPPPN-MRNA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05026">
        <property type="entry name" value="DCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01125">
        <property type="entry name" value="DCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140586">
        <property type="entry name" value="Dcp2 domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="10">
        <location>
            <begin position="103"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="370"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="522"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="607"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="675"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="379"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="396"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="425"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="480"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="499"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="541"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="568"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="623"/>
            <end position="662"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ85301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="736" mass="81369" checksum="3F00A393F3536A9E" modified="2010-06-15" version="1">MDLTNSYIFSNSTLAEILDDLCVRFIINLPEEELSSIERICFQIEEAQWYFEDFIRETNPMLPTLPLRTFLNKIFAICPLPILKEMPPERAENAFAEFMAYKTRVPVRGAVLLNSAMDKCVLVKGWKSGASWSFPRGKINKDERDEDCAAREVLEETSYDIEGRVHSDHFVEVTMREQNMRLYIIPGVPEETKFEPRTRKEISKIAWHHLSDLPTFSKKKNQQNIQQSDVRTGKYYMVAPFLKELRKWISTSGKKWLEEQKQMPSSATLIGDETEIEDIIPPQAVDSSAELRNLLGIPHNGAAKPNGNYNTTGTDEATARLKNFLNLSGPVTEPVDASSKLKSLLNVGGGSNTQITEQSLKPNPQMLLSILQNKGGPSNPQPTATPPILPQPMGQHTPSIPTPTHHSPTGPPNNLMEGFHSQIRPPHPPSPPPTFSFLGPHQMYDPLHSQPHPPNHLFTHRQFNQPIFPSNHHGESPTFPNRPPGWRPSPTPLDALMPSPVPSKPPDPGQAVMLLSILKSAAPSTPATPHTPPPARAANPSRTPSQPYHQQEQQTKQIGLQTPPALKTSGSQHKNNLLDTLRGSTTETTRPASATLIAQYDFPPRHMAPLASENAPARPLHSATASPQISRFPSRTPNASHTPVPASTTPQPTSFDRRQSVSSEQQRALLAIFTNGASTPHEDPAIISSSEGAKPTTPLRSRTTSFARRVTPMSPLSREKDGLLAYLETVAKEGGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLD0</accession>
    <name>D5GLD0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00010136001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430348">
        <property type="protein sequence ID" value="CAZ85323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841132.1">
        <property type="nucleotide sequence ID" value="XM_002841086.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLD0"/>
    <dbReference type="GeneID" id="9186067"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010136001"/>
    <dbReference type="eggNOG" id="ENOG502SWGH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1180946_0_0_1"/>
    <dbReference type="InParanoid" id="D5GLD0"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="141"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="181"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="235" mass="25619" checksum="23C191739581FD42" modified="2010-06-15" version="1">MLHAVFFQTADVFNTPARQQPGAVMYTNAQVRTSIPICNERFQYALDQLEMEILTAKAALRRDLTALRTAAQAREEARLAAERARLEEQRKREAEIAARKEAEERARREKERKEEEERKRRKEAEERERARKAEEEEAAVKRAAAGQSAGTVVVGGGGAGYSPLFGGDKDGKENPAPLPPGSNNASASAAATATGGDSYDDFMASVAAGGYMDGQPIQFMDDFLGEFGDSVFPGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLI3</accession>
    <name>D5GLI3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00010219001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430349">
        <property type="protein sequence ID" value="CAZ85376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841185.1">
        <property type="nucleotide sequence ID" value="XM_002841139.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLI3"/>
    <dbReference type="STRING" id="656061.D5GLI3"/>
    <dbReference type="GeneID" id="9186120"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010219001"/>
    <dbReference type="eggNOG" id="ENOG502QSAF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016177_0_0_1"/>
    <dbReference type="InParanoid" id="D5GLI3"/>
    <dbReference type="OMA" id="LMVQWRQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055334">
        <property type="entry name" value="PEX8-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39214">
        <property type="entry name" value="MICROBODY (PEROXISOME) BIOGENESIS PROTEIN PEROXIN 8 (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39214:SF1">
        <property type="entry name" value="MICROBODY (PEROXISOME) BIOGENESIS PROTEIN PEROXIN 8 (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26001">
        <property type="entry name" value="Pex8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ85376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="647" mass="71168" checksum="31B755D229F05791" modified="2010-06-15" version="1">MVVDRRLDNILRILLQPGAADPNNADASSVYASAATVLATLTNPLNVSLLSTQILISPAIWEENFDGLKAGLRVFGVFQSATVGKFETQGSILSVEEWISALVRGANNNVPRWKHLLLLGGVLSADQERSQLPRVTRTSLEQAFCRAVNLSVESNARHELEADVLSLQKRLSTTMQALLPVIVKSMYNSREGFQSGYFLSTIDHDIMTIEGKLVWPSKSNSFLELQQRSGRPLFAAMSGLARLAAASIRHSQDPMVLHQFVDSLWGFSQVLSAQWKMNGLSEIPMSEEKNRVDEESLKATLPVVWQILKAILFGSTMILQELTARIAESPLLSGPDHGPTFSSKILLILRNFYFITSRLGPNAFSSYNFVYLSAIDILTTYPIPSEKFIIDIAPAQAGTIPPTLPERLFDLYFLNTVEHFTHSLPQKINEETTLPCTSVYLLPSSDASLHAHFESAHSVMLSIIGAPKSADLGARILPFYVDTVFKTFPASLSARQFRLAFGTLIRECSPPQAISSLQPHLAEVIMEILAERAKIASTEPLDNKVDEGAVGLSEKDVCILALIDGLPAMEVGVMERWLDPTADLLNSVGNEGSKGRIRERFWDVLSGELDVERAEVAVRWWTSANGRDKVLLGLGKGEGVEGIRAML</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLN7</accession>
    <name>D5GLN7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00010288001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the tRNA pseudouridine synthase TruA family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430350">
        <property type="protein sequence ID" value="CAZ85430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841239.1">
        <property type="nucleotide sequence ID" value="XM_002841193.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLN7"/>
    <dbReference type="FunCoup" id="D5GLN7">
        <property type="interactions" value="696"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GLN7"/>
    <dbReference type="GeneID" id="9182733"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010288001"/>
    <dbReference type="eggNOG" id="KOG2554">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014673_2_3_1"/>
    <dbReference type="InParanoid" id="D5GLN7"/>
    <dbReference type="OMA" id="SCRERRY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990481">
        <property type="term" value="P:mRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02569">
        <property type="entry name" value="PseudoU_synth_ScPus3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.660">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.580">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00171">
        <property type="entry name" value="TruA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001406">
        <property type="entry name" value="PsdUridine_synth_TruA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020097">
        <property type="entry name" value="PsdUridine_synth_TruA_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020095">
        <property type="entry name" value="PsdUridine_synth_TruA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041707">
        <property type="entry name" value="Pus3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020094">
        <property type="entry name" value="TruA/RsuA/RluB/E/F_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00071">
        <property type="entry name" value="hisT_truA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142:SF5">
        <property type="entry name" value="TRNA PSEUDOURIDINE(38_39) SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01416">
        <property type="entry name" value="PseudoU_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Pseudouridine synthase I TruA alpha/beta" evidence="5">
        <location>
            <begin position="212"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="113"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="454"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ85430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="483" mass="54907" checksum="B008D295ADEBAC0D" modified="2010-06-15" version="1">MSRIKVIHPKKPKRKFDPSLYSTRHIALRIAYLGGNYTGFEYHANSPNTTNTIEEHIFKALLKARLVPCNGIEGEEETVLGWPGDEVAEYSKCGRTDTGVSAFGQVIGVRVRSNRPLPKKPTDDVAEEGGEEGKEKTEFDDEKDELPYPQILNRLLPKDIRVLAWCPHPPPTFSARFNCQQRHYRYFFTNPAIVPSPNSTVGNLDIDKMREAAKLYVGDHDFRNFCKLDASKQITRFDRVIHHADIVELPQSSPNNNPKVYYFELHGSAFLWHQVRHMIAILFLIAQGLEQPSLVTEMLDISKNPRKPMYDIANDRALVLWDCIFPDGMLQWLYARGTGLEGRDELLDDVWSSWHKSKIDEILTNGLMKIVSINGDPTSNPALQSAPPMLCLGELPATAQAMGVAKRKAKGRSQILVEGGHEPLFRGMYIPVLERKRMDDIEVINARYVEKKGDWQENRKKREAKRKAAGVSEEEIMRTSESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5GM63</accession>
    <name>D5GM63_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA-directed RNA polymerase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00010542001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">DNA-dependent RNA polymerase which catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430352">
        <property type="protein sequence ID" value="CAZ85606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841415.1">
        <property type="nucleotide sequence ID" value="XM_002841369.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GM63"/>
    <dbReference type="FunCoup" id="D5GM63">
        <property type="interactions" value="1019"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GM63"/>
    <dbReference type="GeneID" id="9187390"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010542001"/>
    <dbReference type="eggNOG" id="KOG3298">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085878_2_0_1"/>
    <dbReference type="InParanoid" id="D5GM63"/>
    <dbReference type="OMA" id="TMRQPGL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990328">
        <property type="term" value="C:RPB4-RPB7 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003968">
        <property type="term" value="F:RNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060213">
        <property type="term" value="P:positive regulation of nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045948">
        <property type="term" value="P:positive regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010590">
        <property type="term" value="P:regulation of septum digestion after cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04329">
        <property type="entry name" value="RNAP_II_Rpb7_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04462">
        <property type="entry name" value="S1_RNAPII_Rpb7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000043">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB7"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RNA polymerase Rpb7-like, N-terminal domain"/>
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        <property type="entry name" value="Rpb7-like"/>
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        <property type="entry name" value="S1_domain"/>
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        <location>
            <begin position="81"/>
            <end position="162"/>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="EMBL" id="CAZ85606.1"/>
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    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="180" mass="20449" checksum="CD27FA5ADC6D5DCF" modified="2010-06-15" version="1">MFFIHELNRTITLHPSYFGPHMRDYLIHKLHEDVEGSCTGRYFIICVIEYENITLGRIIPGKGDAEFTITYKAVVWRPFKGETVDGVVSSVNKMGFFAEVGPLTVFVSSHLIPRDIKFDPTSNPPQFSDNGDQIINKGAHIRIKLMGTRSDVGKMHAIGSIKEDYLGYDIPNYVEMFVFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMD4</accession>
    <name>D5GMD4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00010659001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_002841440.1"/>
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        <property type="interactions" value="1267"/>
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    <dbReference type="KEGG" id="tml:GSTUM_00010659001"/>
    <dbReference type="eggNOG" id="KOG1795">
        <property type="taxonomic scope" value="Eukaryota"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="term" value="C:catalytic step 2 spliceosome"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="C:Prp19 complex"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
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        <property type="term" value="P:generation of catalytic spliceosome for second transesterification step"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="P:mRNA 3'-splice site recognition"/>
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    </dbReference>
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    </dbReference>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd08056">
        <property type="entry name" value="MPN_PRP8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13838">
        <property type="entry name" value="RNase_H_like_Prp8_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Pre-mRNA-processing-splicing factor 8"/>
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        <property type="entry name" value="Pre-mRNA-processing-splicing factor 8"/>
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    </dbReference>
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        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
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    </dbReference>
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        <property type="entry name" value="Pre-mRNA-processing-splicing factor 8, U5-snRNA-binding domain"/>
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        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
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        <property type="entry name" value="PRO8NT"/>
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        <property type="entry name" value="PROCN"/>
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        <property type="entry name" value="PROCT"/>
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        <property type="entry name" value="PRP8"/>
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        <property type="entry name" value="PRP8_domainIV"/>
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        <property type="entry name" value="Prp8_domainIV_fingers"/>
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        <property type="entry name" value="Prp8_domainIV_palm"/>
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        <property type="entry name" value="Prp8_U5-snRNA-bd_sf"/>
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        <property type="entry name" value="Prp8_U6-snRNA-bd"/>
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        <property type="entry name" value="RNaseH-like_sf"/>
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        <property type="entry name" value="RRM_spliceosomal_PrP8"/>
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        <property type="entry name" value="PRE-MRNA SPLICING FACTOR PRP8"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RRM_4"/>
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        <property type="entry name" value="Ribonuclease H-like"/>
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    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="MPN" evidence="8">
        <location>
            <begin position="2122"/>
            <end position="2257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="22"/>
            <end position="34"/>
        </location>
    </feature>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
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        <source>
            <dbReference type="EMBL" id="CAZ85677.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="2359" mass="273602" checksum="2970C4DF1E652D56" modified="2010-06-15" version="1">MASLPPPPPPGWQGVSRSGAPLQPPPPGYRPPADPQQAKFDQKKKEWLRIQKNRFGEKRKNGFVDTHKADMPPEHLRKIVRDIGDVSQKKFSADKRAYLGALKFMPHAVLKLLENMPMPWESAREVKVLYHVNGCLTLVNEIPRVIEPVFHAQWATMWVCMRREKSDRRHFKRMRFPPFDDEEPPLSWSENIEDVEPQEPIQMELDEHEDAAVVEWFYDHRPLLDTPHVNGPGYKRWNLDLSQMATLYRLSQQLLSDLVDKNYFYMFDRKSFFTAKALNVAIPGGPRFEPLYKDIDPNDEDFGEFNAIDRIIFRSPIRTEYRVAFPFLYNSLPRSVHLSWYHYPQITYVRAEDPELPAFYFDPVINPISSRSVAPANLTVSHEDEVFGYGNDEDDFQLPDEVEPFMAEEALYTENTTSAIALWWAPYPFDRRSGKTVRAEDVPLVKQWYLEHCPQNQPVKVRVSYQKLLKTYVLNALHTRSPKAQNKQDLFRTLKGTKFFQTTTIDWVEAGLQVCRQGFNMLNLLIHRKNLTYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLMREILRLTKLIVDAQVQYRLGNIDAFQLADGILYAFNHVGQLTGMYRYKYKLMHQIRACKDLKHLIYYRFNSGPVGKGPGCGFWAPAWRVWLFFMRGILPLLERWLGNLLARQFEGRHSKGVAKTVTKQRVESHFDLELRAAVIHDITDMMPAGMKSNKVNTVLQHLSEAWRCWKSNIPWKVPGLPAPIENVILRYVKSKADWWVSVAHYNRERIRRGATVDKTVAKKNLGRLTRLWLKAEQERQHNYMKDGPYVSSEEAVAIYTAAVHWLEARKFSPIPFPSVSYKHDTKILILALERLREAYSVKGRLNQSQREELALIEQAYDSPGTTLTRIKTALLTRRAFKEVGIDMNDNYSTINPVYDVEPIEKITDAYLDAYLWYQADQRHLFPAWIKPSDSEVPPLLVYKWAQGINNLHGVWETADGECNVLLETQLSKVYEKIDITFLNRLLRLIMDHNLADYITAKNNVVLNYKDMNHTNMYGLIRGLQFSAFVFQYYGLILDLLILGLERASEIAGPPQRPNEFLQFQDLEAETKHPIRLYTRYVDRIWVFFRFSADDARDLIQRFLTEQPDPNFENVIGYRNKKCWPRDSRMRLMRHDVNLGRAVFWDLKNRLPRSVTTIEWEDTFASVYSRDNPNLLFSMSGFEVRILPKTRNQNEEFPVKDSVWSLVDNNSKERTAHAFLRVTEDDIAKFNNRIRQILMSSGSTTFTKIANKWNSALIALFTYYREAAISTEALLDTIVKCETKIQTRVKIGLNSKMPSRFPPAVFYTPKELGGLGMISGSHILIPTSDRRWSKQTDAGITHFRSGMTHDDDVLIPNIFRYIQPWEAEFIDSARVWSEYAQKRAEANQQNRRLTLEDLEDSWDRGIPRINTLFQKDRSTLAFDKGWRVRTEFKIYSMMRSNPFWWTSQRHDGKLWNLNAYRTDVIQALGGVETILEHTLFKATAFPSWEGLFWEKASGFEESMKFKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYIGFQVQLDLTGIFLHGKIPTLKISLIQIFRAHLWQKIHESVTMDLCQVFDQELEALGIETVQKETIHPRKSYKMNSSCADILLFAAYKWNVTRPSLLHDQKDVIEQNATNKFWIDVQLRYGDYDSHDVSRYVRAKFLDYTTDSLSIYPSPTGVMIAIDLAYNLYDAYGQWFPGLKQLVQQAMAKIMKANPALYVLRERIRKGLQLYASESNQEFLNSQNYSELFSNQIQLFIDDTNTYRVTVHKTFEGNLTTKPINGAIFILNPRSGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQLIVTRKGLLDPLEVHLLDFPNISIRASELQLPFQAAMKIEKLGDMILRATEPQMVLFNLYDDWTKTVSSYTAFSRLILILRALHVNQDKTKLLLRPDKTVITQEHHIWPTLSDDDWIKVETQLRDLILNDYGKKNNVNVSSLTSSEVRDIILGMEISAPSMQRQQAAEIEKQQQEQAQLTAVTTRTQNVHGDEMIVTTTSNYEQQAFASKTEWRSRAIAASNLRTRSNQIFISSDDISDNSQHTYIMPKNILKRFIMIADLRMQVAGYVYGSSPPDNDQVKEIRCIAMVPQIGSLNTVQLPHYLPQHDYLTKENGLEPLGWIHTWAQPNETPYLNAFDTTQHARLMSAHKEWDKKTITMTVAFKAGSVSLAAYGLTPAGYEWGAQNKDLGNDMPPGFQTNMGEKCQLLLSDKIMGFFLVPESGVWNYSFLGANFSTEAEKKAYHVKIDTPVGYYADIHRPLHFQNFAELEDIWVDRSDNFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMG2</accession>
    <name>D5GMG2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00010691001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430354">
        <property type="protein sequence ID" value="CAZ85705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841514.1">
        <property type="nucleotide sequence ID" value="XM_002841468.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMG2"/>
    <dbReference type="GeneID" id="9182131"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010691001"/>
    <dbReference type="HOGENOM" id="CLU_2795795_0_0_1"/>
    <dbReference type="InParanoid" id="D5GMG2"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ85705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="68" mass="7698" checksum="37B7532485C55660" modified="2010-06-15" version="1">MEGRSFSPFLQWGDQRRCGVTLKRHLPGGSILVPYSAGMIPCLYHTSTYDTLYDTSIILCRVYSVPQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMI4</accession>
    <name>D5GMI4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00010743001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 15 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430356">
        <property type="protein sequence ID" value="CAZ85727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841536.1">
        <property type="nucleotide sequence ID" value="XM_002841490.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMI4"/>
    <dbReference type="FunCoup" id="D5GMI4">
        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GMI4"/>
    <dbReference type="GeneID" id="9182153"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010743001"/>
    <dbReference type="eggNOG" id="KOG4472">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024327_4_1_1"/>
    <dbReference type="InParanoid" id="D5GMI4"/>
    <dbReference type="OMA" id="GYRHNPF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000051">
        <property type="entry name" value="Alpha-1,2-mannosyltransferase (Ktr4)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002685">
        <property type="entry name" value="Glyco_trans_15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121">
        <property type="entry name" value="ALPHA-1,2 MANNOSYLTRANSFERASE KTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121:SF6">
        <property type="entry name" value="ALPHA-1,2 MANNOSYLTRANSFERASE KTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01793">
        <property type="entry name" value="Glyco_transf_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018153">
        <property type="entry name" value="Glyco_trans_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072873" evidence="6">
        <location>
            <begin position="27"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="42"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR018153-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ85727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="403" mass="47184" checksum="10412EA6315E1C1F" modified="2010-06-15" version="1">MAANSRTLRFILLAFFSIAVLFKVYTSNYHAGTPLVDVEKIAEQQHAQDSSSSPSQSNGNNGFSSNPASGLPAGVHAGKRANATFVTLARNSDVWEIAKSIRHVEDRFNRKFNYDWVFLNDKPFDSQFKKVTSALTSGKAKYGLIPHEHWSFPDWIDQDKAKKVREDMAQRKIIYGDSIPYRHMCRYESGFFFRHELLLDYEYYWRVEPSIELFCDVDYDVFRFMAENKKKYGFVLSLYEYVETIPTLWDSVKSFMKEHPEYINPNNAMGFLSDDGGETYNHCHYWSNFEVGSLDFWRDKAYMDFFEKLDKDGGFFYERWGDAPVHSIAASLFLDKSQIHFFDDIAYYHVPFTHCPTSEKDRLKHKCHCNPKDNFDWKGYSCLSRHFEINSLEKPEGYETQMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMJ0</accession>
    <name>D5GMJ0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00010749001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430356">
        <property type="protein sequence ID" value="CAZ85733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841542.1">
        <property type="nucleotide sequence ID" value="XM_002841496.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMJ0"/>
    <dbReference type="STRING" id="656061.D5GMJ0"/>
    <dbReference type="GeneID" id="9182159"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010749001"/>
    <dbReference type="eggNOG" id="KOG2687">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_545357_0_0_1"/>
    <dbReference type="InParanoid" id="D5GMJ0"/>
    <dbReference type="OMA" id="ARNEAMQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034993">
        <property type="term" value="C:meiotic nuclear membrane microtubule tethering complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043495">
        <property type="term" value="F:protein-membrane adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045119">
        <property type="entry name" value="SUN1-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012919">
        <property type="entry name" value="SUN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12911:SF8">
        <property type="entry name" value="KLAROID PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12911">
        <property type="entry name" value="SAD1/UNC-84-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07738">
        <property type="entry name" value="Sad1_UNC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51469">
        <property type="entry name" value="SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="SUN" evidence="6">
        <location>
            <begin position="279"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="245"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ85733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="500" mass="55973" checksum="C58B7C660B36D501" modified="2010-06-15" version="1">MESSTDRDKIRNEIDSLSSTLDTVRVLQDAMLEDNRRNLDSLLSAQQALDRELSDFRALQKARNEAMQSFEEALPKQMVATLGPNGKVQLTDEFQDSLQDVFSKIFPKHFNDAIAKTGPSGIGKIPSWEAFMKGNEDKLKDLIQKHASAGDGVGKNGKPGGVVLSKAFVMAMIQEEAEKYHKKWEVETFLPDFESKFESQLEELQRRIRRENTEHFSSASSSILAAASAIASGTANRAARNMAQEFADSRGGRGTSGRISQGGKERWTTLPDYASIITGASIWPYITSPSYDWTGGRGYHHFVWRLFGRGARISPPPALAITPSTEVGECWPFPERSGDIGIKLAKPIYVSHVTIDHVPKQQAIEISSAPKNIEFWIRVPAERKEELQKAVGKPASEWFRQDSDTQKIQQQQLQMSGNNGGEWVRVHEFMYDIHTAGSPVQTFELPVDLTRLNVTSHFVAFRIVDNWGHPNFTCLYRVRVHGYPPKRDLPIGDGERAEGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5GN18</accession>
    <name>D5GN18_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00011051001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430362">
        <property type="protein sequence ID" value="CAZ85911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841720.1">
        <property type="nucleotide sequence ID" value="XM_002841674.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GN18"/>
    <dbReference type="STRING" id="656061.D5GN18"/>
    <dbReference type="GeneID" id="9182199"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011051001"/>
    <dbReference type="eggNOG" id="ENOG502S2VJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021697_0_0_1"/>
    <dbReference type="InParanoid" id="D5GN18"/>
    <dbReference type="OMA" id="MTARIIF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000444">
        <property type="term" value="C:MIS12/MIND type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013218">
        <property type="entry name" value="Dsn1/Mis13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14778">
        <property type="entry name" value="KINETOCHORE-ASSOCIATED PROTEIN DSN1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14778:SF2">
        <property type="entry name" value="KINETOCHORE-ASSOCIATED PROTEIN DSN1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08202">
        <property type="entry name" value="MIS13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="178"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="469"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="242"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="276"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="291"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="331"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ85911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="620" mass="67576" checksum="E84D5E1888610B12" modified="2010-06-15" version="1">MMPASSSRPKRKSRGDAYLEDNGIGMGGGNGRKEKENSAPAVKKRREEDKQSSKSTATAVTTATSASMTGSTATATKPKVGSLLFLCTIHSLFYSLLWQSAEGGEGELQLLGAVVIYSFVFRTDWERAELLSRVWADCTLQKKKKTAATEMRLDDGFAYRVRTTRSSTAAAAAAAQESIQSIASSSHDTAPIAPPPDPPRQRVKKKRVMVSPPPEEAGSKPKVAKANKGKTIPLSQNGTREVEEEVGKEEEQEGQEQEEEDKKKEKGAAPKASGASKEKKAAPGERRPKITASSTPRLQKSQIPTAVGGGHGPSDGTKVVLPVSDTPIIRRNQQLRQKGQGNRRSSVGMRGRRASSLMDNGVVGMTARIIFPSLAPHAEVETEEFYKHIADELLEPQRMKQLLSWCGKRALSHQTSPAAAGVDGNARAVARIIEEEVLKDLLSNAELSSWFNRDDSQAPPEVKKPNPRNLDNLAKVEECEANLKKLREERETWGSLLRPETQSTLSISGGKSEVGLFEKSLLRPEESEFANSLAKSQDLLGLAKRLVKQQCGEIEFQVDQLIDGIHKLNQYGEAADRLGGRILEDAESTLAVREATLRQASGTEALPIQEVLRSLSRMDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNF7</accession>
    <name>D5GNF7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00011272001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430364">
        <property type="protein sequence ID" value="CAZ86050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841859.1">
        <property type="nucleotide sequence ID" value="XM_002841813.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNF7"/>
    <dbReference type="GeneID" id="9182250"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011272001"/>
    <dbReference type="HOGENOM" id="CLU_1662086_0_0_1"/>
    <dbReference type="InParanoid" id="D5GNF7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ86050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="159" mass="16546" checksum="6AE974B368AF0C90" modified="2010-06-15" version="1">MSTKASPRPVVIVQNTPSSSASSLISTASITSSITRSNSVSSTHSSNTSTSSASQHHAEGLVSRTRPARAVGNGREFVETGHPQGGNPHGGPPREENGYYRVSTESVTVHNSGGLLTGSVSTDMGEAYANGLDHTENCRKLSQKSDNRTLCFCPASPST</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNS4</accession>
    <name>D5GNS4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00011410001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430367">
        <property type="protein sequence ID" value="CAZ86140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNS4"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011410001"/>
    <dbReference type="HOGENOM" id="CLU_2623792_0_0_1"/>
    <dbReference type="InParanoid" id="D5GNS4"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ86140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="78" mass="8651" checksum="0FB41D919425A6A5" modified="2010-06-15" version="1">MACCQTLRAWWGRGYWRGDVVCDVIFDGWVHGVYHGSMTCGSDIAFALFICRTHIGSEGAGLFPLILFVHYLGLLIGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNX2</accession>
    <name>D5GNX2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00011561001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430371">
        <property type="protein sequence ID" value="CAZ86215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842024.1">
        <property type="nucleotide sequence ID" value="XM_002841978.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNX2"/>
    <dbReference type="GeneID" id="9183508"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011561001"/>
    <dbReference type="HOGENOM" id="CLU_1046594_0_0_1"/>
    <dbReference type="InParanoid" id="D5GNX2"/>
    <dbReference type="OMA" id="EYQHAIT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="157"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ86215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="266" mass="29395" checksum="225E6DDABC7A09C8" modified="2010-06-15" version="1">MLTFIAVRCLNCNNVDQFPRMDFDEIPYCSQCAANSNLVPATPPAKPDQTWASEDELASLFSRNLSLLDDAPAARSPTPPGASPTGINFSISQHYIQPTTFGKPAADKPHESAAGNSNADRERTYIQYLLSSNLGSETSTNDLEIALAHGIIDSNMYHQALETHNQLLRQREQQEAEARMIQAQIMNEMQREQSRQATTALGEDCSAHSLLSIASRQKTQDPVFRTSDFFNDYYGGVDSGYLDDDEYAPCSVYGGQGSCHLRPFDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>D5GP01</accession>
    <name>D5GP01_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00011604001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protease inhibitor I9 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430371">
        <property type="protein sequence ID" value="CAZ86244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842053.1">
        <property type="nucleotide sequence ID" value="XM_002842007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GP01"/>
    <dbReference type="FunCoup" id="D5GP01">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="MEROPS" id="I09.003"/>
    <dbReference type="GeneID" id="9183537"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011604001"/>
    <dbReference type="eggNOG" id="ENOG502SC39">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_156026_0_1_1"/>
    <dbReference type="InParanoid" id="D5GP01"/>
    <dbReference type="OMA" id="IMASEWE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004866">
        <property type="term" value="F:endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052471">
        <property type="entry name" value="PBI_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28288:SF1">
        <property type="entry name" value="INHIBITOR I9 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28288">
        <property type="entry name" value="PROTEASE B INHIBITOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072860" evidence="2">
        <location>
            <begin position="18"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ86244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="95" mass="10344" checksum="30E1120C81D25E19" modified="2010-06-15" version="1">MQLKYLVALLLPALAAAEKCLNYVITFQPNTPDSVIEQAIATVKDNGGRVTHVYKIIKGFCVCAPHEALNQVSIMASEWEPVIEEDTVITTVGIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>D5GP75</accession>
    <name>D5GP75_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00011739001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430373">
        <property type="protein sequence ID" value="CAZ86340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842149.1">
        <property type="nucleotide sequence ID" value="XM_002842103.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GP75"/>
    <dbReference type="FunCoup" id="D5GP75">
        <property type="interactions" value="226"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GP75"/>
    <dbReference type="GeneID" id="9186580"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011739001"/>
    <dbReference type="eggNOG" id="ENOG502RG16">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015714_0_0_1"/>
    <dbReference type="InParanoid" id="D5GP75"/>
    <dbReference type="OMA" id="DRAEEWC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013927">
        <property type="entry name" value="TF_Opi1_Ccg-8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38406">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR OPI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38406:SF1">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR OPI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08618">
        <property type="entry name" value="Opi1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="213"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ86340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="251" mass="27597" checksum="2B6171509B5BDB34" modified="2010-06-15" version="1">MNGGSDRALDEGASDRQIAAPNQTWPTRVVLSTYGFGAALNEESLKNLKYCLDWLRFLNAHLGKLVSALKGVVEELDDHRRSQMDEDRHANGGIARALPTSYENSRAVLAAKVESLKGDVVATMKKVVEVVSNYTGSALPENARELIKRHIFALPQRFQAANASELAEKEKDGPMSSASRALVLAKEGLDMMQQVSAVVEGTIDRAEEWCERLGRKKREDDGQSGGEEKKVEWAEGGRDGDEDVKMEKPRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>D5GP80</accession>
    <name>D5GP80_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00011745001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430373">
        <property type="protein sequence ID" value="CAZ86345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842154.1">
        <property type="nucleotide sequence ID" value="XM_002842108.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GP80"/>
    <dbReference type="GeneID" id="9186585"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011745001"/>
    <dbReference type="eggNOG" id="ENOG502SEV8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104756_1_1_1"/>
    <dbReference type="InParanoid" id="D5GP80"/>
    <dbReference type="OMA" id="ACLCKWH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="126"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ86345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="150" mass="14810" checksum="1391694231174DA6" modified="2010-06-15" version="1">MFERNKGRGLNIPIETCISQYSPRVSGCSQNDYSCLCVEYTNLLTCYNNCPKDTNSSGIKSSVTSFCNAAAAASSASALTNTAKSTSGTGSSTAASSAPTGSGSPTTTKAPSSSSSSSSGSGSSNAALALTPVGTYGALAGVMAIAGIFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GP85</accession>
    <name>D5GP85_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00011750001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430373">
        <property type="protein sequence ID" value="CAZ86350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842159.1">
        <property type="nucleotide sequence ID" value="XM_002842113.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GP85"/>
    <dbReference type="GeneID" id="9186590"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011750001"/>
    <dbReference type="HOGENOM" id="CLU_2639897_0_0_1"/>
    <dbReference type="InParanoid" id="D5GP85"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ86350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="77" mass="9281" checksum="A8C732BC9696BE9C" modified="2010-06-15" version="1">MYDAVLERPSWDIRWRICWREGGGKALWYCNRQLRWSMRYDSYGTCVHSSPRTAARMRISNLLFIENFKMPSYSATH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>D5G3U6</accession>
    <name>D5G3U6_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cleavage and polyadenylation specificity factor subunit 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00003809001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the cleavage factor Im (CFIm) complex that functions as an activator of the pre-mRNA 3'-end cleavage and polyadenylation processing required for the maturation of pre-mRNA into functional mRNAs. CFIm contributes to the recruitment of multiprotein complexes on specific sequences on the pre-mRNA 3'-end, so called cleavage and polyadenylation signals (pA signals). Most pre-mRNAs contain multiple pA signals, resulting in alternative cleavage and polyadenylation (APA) producing mRNAs with variable 3'-end formation. The CFIm complex acts as a key regulator of cleavage and polyadenylation site choice during APA through its binding to 5'-UGUA-3' elements localized in the 3'-untranslated region (UTR) for a huge number of pre-mRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer (via N- and C-terminus); binds RNA as homodimer. Component of the cleavage factor Im (CFIm) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Nudix hydrolase family. CPSF5 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835068.1">
        <property type="nucleotide sequence ID" value="XM_002835022.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G3U6"/>
    <dbReference type="STRING" id="656061.D5G3U6"/>
    <dbReference type="GeneID" id="9187127"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003809001"/>
    <dbReference type="eggNOG" id="KOG1689">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068704_0_0_1"/>
    <dbReference type="InParanoid" id="D5G3U6"/>
    <dbReference type="OMA" id="NDEWEIG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005849">
        <property type="term" value="C:mRNA cleavage factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18871">
        <property type="entry name" value="NUDIX_Cfim25_Nudt21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000005">
        <property type="entry name" value="Cleavage and polyadenylation specificity factor subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016706">
        <property type="entry name" value="Cleav_polyA_spec_factor_su5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13047">
        <property type="entry name" value="PRE-MRNA CLEAVAGE FACTOR IM, 25KD SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13869">
        <property type="entry name" value="NUDIX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017888">
        <property type="entry name" value="CPSF-25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="236"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR017888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ79189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="261" mass="29572" checksum="80495B7E00E40012" modified="2010-06-15" version="1">MSTITSTALQSSQAPILPASFSANQPKTIRLYPLSNYTFGTKENQPEEDPSVLARLQRLQEHYEQYGMRRTCEGILVCHEHCHPHILMLQIANAFFKLPGDYLHHHESELEGFKSRLNERLAPVGSQFSGEGVNDEWEIGDTLAQWWRPNFETFMYPFIPAHVTRPKECKKLYFIQLPRSKVLSVPKNMKLLAVPLFELYDNTARYGPQLSAIPHLLSRYNFEFVDENGKVIAVTPGGGPDGEPQTKVLAGGEENGDIKTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5G433</accession>
    <name>D5G433_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein mS29</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00003930001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mS29 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835155.1">
        <property type="nucleotide sequence ID" value="XM_002835109.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G433"/>
    <dbReference type="FunCoup" id="D5G433">
        <property type="interactions" value="89"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G433"/>
    <dbReference type="GeneID" id="9182403"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003930001"/>
    <dbReference type="eggNOG" id="KOG3928">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046315_1_0_1"/>
    <dbReference type="InParanoid" id="D5G433"/>
    <dbReference type="OMA" id="GLAHWMT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019368">
        <property type="entry name" value="Ribosomal_mS29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12810">
        <property type="entry name" value="MITOCHONDRIAL 28S RIBOSOMAL PROTEIN S29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12810:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10236">
        <property type="entry name" value="DAP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="33"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ79276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="462" mass="51407" checksum="54DEB36E6EC6C297" modified="2010-06-15" version="1">MVPPTLWRCLRTPYITHPLKLRPCTHNFTTTLSLSAKPLKPKGPPPSSKMNPGKQRTQSSKLRIKKAPIAVQKPAYEVGQRKELRRRIVLSNTNAIPVALPPMTADLVGNEEAAGAMFALPGGEGDMIVDRLRALGAFQTKQHWPFFHRPCTLFRRETVEIGRMLERVMKREVVGEDGKANERAILHGYYGCGRSILLLQAMTWALQMEWVVIAIPNAHDLVVGHTEYELEGNSHLWLQKAYTTDLLNRVLKANQSAFKQMKLSQNYRLGRMDEAQKGDAMKRVLETAVLDPLLAWDAFNVLLGELGLPGSPPVLFTLDNLNQISIPSKYRDPAFNAIHPHDLALPNTFLAYLSGKRSFRTGITLAATSSKCSRTLALDAALEGRGVDPYEKLDPRIGPSIEGAQIIQVDSLSKREAKALMDYYSESGIMKAPVDIATFSEKYAVSAGNPRDLFGTCLRVRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4Q1</accession>
    <name>D5G4Q1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="12">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00000037001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="FN429990">
        <property type="protein sequence ID" value="CAZ79487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835330.1">
        <property type="nucleotide sequence ID" value="XM_002835284.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4Q1"/>
    <dbReference type="FunCoup" id="D5G4Q1">
        <property type="interactions" value="9"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G4Q1"/>
    <dbReference type="MEROPS" id="S08.052"/>
    <dbReference type="GeneID" id="9183005"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000037001"/>
    <dbReference type="eggNOG" id="KOG1153">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011263_2_0_1"/>
    <dbReference type="InParanoid" id="D5G4Q1"/>
    <dbReference type="OMA" id="RHPDVDY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000425">
        <property type="term" value="P:pexophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007039">
        <property type="term" value="P:protein catabolic process in the vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030435">
        <property type="term" value="P:sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04077">
        <property type="entry name" value="Peptidases_S8_PCSK9_ProteinaseK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.80:FF:000006">
        <property type="entry name" value="Autophagic serine protease Alp2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034193">
        <property type="entry name" value="PCSK9_ProteinaseK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050131">
        <property type="entry name" value="Peptidase_S8_subtilisin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806:SF11">
        <property type="entry name" value="CEREVISIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806">
        <property type="entry name" value="PEPTIDASE S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072514" evidence="9">
        <location>
            <begin position="20"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="Inhibitor I9" evidence="11">
        <location>
            <begin position="45"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="10">
        <location>
            <begin position="186"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ79487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="350" mass="36955" checksum="62E65FB9A8500D65" modified="2010-06-15" version="1">MHLSAKFALLLASISVSIASPIHDAALGKPAPLVTSALEHEIPNNYIVVFKKHVDDASATGHHSWVADLHETSISSLRKRSQIPLIRGEVDLSSSVDVNINSLTGLKHTYNISGSLLGYSGAFDESVVEQIRNHPDVAYVEKDTKVYALADPVTEHSAPWGLARISHQESLNFGSYNKYIHQAHGGQGVDVYVIDTGTNIDHNDFEGRAHWGKTIPAGDEDVDGNGHGTHCSGTIAGKKYGVAKKANVYAVKVLRSNGSGTMSDVVKGVEYAAESHVKKAAAAAARKDKNFKGSAANMSLGGGKSSALDQAVNGAVMLGLNFAVAAGNDNADAWPISPTTENVLIFSPLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4S7</accession>
    <name>D5G4S7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00000081001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429990">
        <property type="protein sequence ID" value="CAZ79513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835356.1">
        <property type="nucleotide sequence ID" value="XM_002835310.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4S7"/>
    <dbReference type="STRING" id="656061.D5G4S7"/>
    <dbReference type="GeneID" id="9188449"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000081001"/>
    <dbReference type="eggNOG" id="ENOG502S1EZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056292_0_1_1"/>
    <dbReference type="InParanoid" id="D5G4S7"/>
    <dbReference type="OMA" id="IFRDVMA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025638">
        <property type="entry name" value="DUF4336"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33835:SF1">
        <property type="entry name" value="METALLO-BETA-LACTAMASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33835">
        <property type="entry name" value="YALI0C07656P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ79513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="260" mass="29123" checksum="C8283CDA300609AB" modified="2010-06-15" version="1">MAKTFPESSMVIRAISPNITTLSVPFLRFNKAKFGGRATIVKLPTGGLAVFSPVGLTNDAKSAVENMGGRVSYLIASDIEHHMNLGPWKQEYASATVIGPEGLYEKRKAQGNEDVPIDITYTKENKHSLKLPDELGSVFDVEYFDGHQNKELVFNYKPERTMIQADLVFNLPCYEQFSKSGMDATSGIWTKIAHHFLNIHGKGQQRFHWYVSPANKPSFAESAKVVAGWEFDRIIPCHGEVIENGGNDVFRRIFAWNLKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5A0</accession>
    <name>D5G5A0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00004242001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835536.1">
        <property type="nucleotide sequence ID" value="XM_002835490.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5A0"/>
    <dbReference type="GeneID" id="9188112"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004242001"/>
    <dbReference type="eggNOG" id="KOG2922">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012349_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5A0"/>
    <dbReference type="OMA" id="STWMPWF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008521">
        <property type="entry name" value="Mg_trans_NIPA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570:SF85">
        <property type="entry name" value="DUF803 DOMAIN MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_1G15880)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05653">
        <property type="entry name" value="Mg_trans_NIPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="80"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="157"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="218"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="302"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="306"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ79693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="324" mass="34692" checksum="E19AD05DA9EB77EA" modified="2010-06-15" version="1">MITMILGEIANFAAYAFAPAILVTPLGALSVLVGAVLGTYFLREELGVLGKLGCAICLIGSVIIVLHAPPDEDIQTIDEILYYAIQPGFLAYVAIVSAFSSIMIYKVSPKYGKKNPLIYISICGSVGSLTVMSSKAFGIAVKLTFAGNNQFTHPSTYVFIIVVAVCILTQMNYFNKALSQFSSSLVTPLYYVTFTTATLIASFILFQGFNTTDPVNTISLLCGFLIIFGGVYLLNLSRSDPNGHRLAANGSASYGEGVPTDGITGLQVRRSMQLRRSDVGGSRSSIGARLLERHLDEEAAVGLTDLAEDDDSDSDDGLGRLHNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>D5G664</accession>
    <name>D5G664_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00001792001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430007">
        <property type="protein sequence ID" value="CAZ80007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835850.1">
        <property type="nucleotide sequence ID" value="XM_002835804.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G664"/>
    <dbReference type="GeneID" id="9187686"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001792001"/>
    <dbReference type="eggNOG" id="ENOG502SYQ8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1541227_0_0_1"/>
    <dbReference type="InParanoid" id="D5G664"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033481">
        <property type="entry name" value="Dni1/Fig1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12351">
        <property type="entry name" value="Fig1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="174" mass="17719" checksum="34EF8B51F23576BB" modified="2010-06-15" version="1">MFKKVAAGAAVLAAVTLLGGMVLQNVTSTAVATLVNQLGLTAINVHLGNMIPVFGWTSFALTLLSALGLSAVVVTEYTLEKTQEKITRKAEEALSRGTGGRIGPADVREWKNAVSSINERGGSAGSDTGLVGRVGGGGAGRAAIVGQGVSLATSLMKKKKMNGDGRPVTGHNFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>D5G676</accession>
    <name>D5G676_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00001644001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430008">
        <property type="protein sequence ID" value="CAZ80019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835862.1">
        <property type="nucleotide sequence ID" value="XM_002835816.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G676"/>
    <dbReference type="GeneID" id="9187698"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001644001"/>
    <dbReference type="eggNOG" id="ENOG502SY3V">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_183924_2_0_1"/>
    <dbReference type="InParanoid" id="D5G676"/>
    <dbReference type="OMA" id="QSPFSEW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ80019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="60" mass="6285" checksum="1BFDEE456F304821" modified="2010-06-15" version="1">MPPKRSLAPAGSPPQRGLISSFYHGIKSPENASVVRSVAVFAAAAAFLSSSWSDYLLPPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>D5G697</accession>
    <name>D5G697_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00001666001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RMD1/sif2 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430008">
        <property type="protein sequence ID" value="CAZ80040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835883.1">
        <property type="nucleotide sequence ID" value="XM_002835837.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G697"/>
    <dbReference type="FunCoup" id="D5G697">
        <property type="interactions" value="172"/>
    </dbReference>
    <dbReference type="GeneID" id="9187711"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001666001"/>
    <dbReference type="eggNOG" id="KOG2861">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011220_3_0_1"/>
    <dbReference type="InParanoid" id="D5G697"/>
    <dbReference type="OMA" id="NERCQVF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003734">
        <property type="entry name" value="DUF155"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051624">
        <property type="entry name" value="RMD1/Sad1-interacting"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16255">
        <property type="entry name" value="REQUIRED FOR MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16255:SF4">
        <property type="entry name" value="SPORULATION PROTEIN RMD8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02582">
        <property type="entry name" value="DUF155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="486"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="domain" description="DUF155" evidence="4">
        <location>
            <begin position="280"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="211"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="9"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="82"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="112"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="211"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ80040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="521" mass="59251" checksum="9162176BDD3D25A3" modified="2010-06-15" version="1">MVRLISVDSVLQESSQIPSGQSRNPLARARGAPASPSCPTTPKPSDPRRFLGQNLPVRSSKLSEKLVLLPETEEEANRWGYGEDDTEDAPIKDDEEEWRSRLEGDEESPEQPPRKSYAERLPKSRRADKLSRVTAYCTADAYRLNATSKFLREKHSARTKLYDECLYCAYHLPIMSGNDGYRVRSSPVLKSPGGKAVLDVQIERSEQRDYHEGYFEDSYTGERRTSGDGSYEEDGHPKSPGKGHGFPQDGRDDHHTYNQGYRPESPRDSNMNDMKAFAEMFVFNYGVVVFWGFSERQEKDILADLTFAQNDFGSQLISGRLKEDEFETEEFHFQYSSRTRSPRIYNDMITLRSGDIMIKLAISHAIAQSTKLCRFEERMNSTMLGVQHIPKKLALTGKLGMKREEVVKMSGKLFKLRVDVNLSSNVLDVPEFFWAAEPTLHPLYAAVGEYLEIKQRILVLNERCRVFLDLTDILSDSIADSNMSRITWIIIILIVLSIFVTTAEVAIRFGLLSARRKAGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6B5</accession>
    <name>D5G6B5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00004407001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430009">
        <property type="protein sequence ID" value="CAZ80058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835901.1">
        <property type="nucleotide sequence ID" value="XM_002835855.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6B5"/>
    <dbReference type="GeneID" id="9187429"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004407001"/>
    <dbReference type="HOGENOM" id="CLU_2442471_0_0_1"/>
    <dbReference type="InParanoid" id="D5G6B5"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="90" mass="9897" checksum="350B051AA762A2A3" modified="2010-06-15" version="1">MIIPVPHSRAERKPQDGSFATKRKKKDAIPSYGTLYCMIPVSSSEPDNLNFRESMLSKTLGLCAVGGPRELDKSLFAEETWPIEPTTSAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6G0</accession>
    <name>D5G6G0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00004466001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the HSF family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430009">
        <property type="protein sequence ID" value="CAZ80103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835946.1">
        <property type="nucleotide sequence ID" value="XM_002835900.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6G0"/>
    <dbReference type="STRING" id="656061.D5G6G0"/>
    <dbReference type="GeneID" id="9181188"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004466001"/>
    <dbReference type="eggNOG" id="KOG0627">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031225_0_0_1"/>
    <dbReference type="InParanoid" id="D5G6G0"/>
    <dbReference type="OMA" id="SALWEFK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000229">
        <property type="entry name" value="HSF-type DNA-binding domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF396">
        <property type="entry name" value="FLOCCULATION SUPPRESSION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00056">
        <property type="entry name" value="HSFDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00434">
        <property type="entry name" value="HSF_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="6">
        <location>
            <begin position="77"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="150"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="269"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="371"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="410"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="418"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="467"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ80103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="508" mass="55882" checksum="45A6800AA30EE41C" modified="2010-06-15" version="1">MSMSASMDTPMGNAPNSGSGLGLGGAAGGVQQPKVQTAFIHKLYNMLEDANIQHLISWSPSNESFVVSPTGEFSKVLSQYFKHTNISSFVRQLNMYGFHKVNDVFHTGSPDATLWEFKHGNGSFKRGDLVGLRDIKRRASRHALIHRDSFSGPKSTVASAPGTPDERPGDSIDGRFISIEQSLYEINSRLARSEDHNAMLGIRLQTAIEGLTRCHQVSPEPLRFISIPFTRLTGREVNSMQKEIERHTQLLEEPQEMLLRERQPFLSSLTIDNSAPLSPRQRPMEDESRRSSITGPLRPTGYFRAPAPPISNAPRRFGSVGLAGGSSPNALRPQQPSQQPPAQHPLGSFHSPPSHLSRRHTSADIRLHGWQANSPFSGMSGSNQWPPSPNRSSNLQHDNTREILANYEIQQHRPPNNPVFTSQTTPPLSETTSNNGISDSGWSFGGGGPKFPPQREAGHTAPPTRRSSLASSSVHALLNPLDSNLSAETNRDGMSDCEPDDRKRKRLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6G6</accession>
    <name>D5G6G6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00004473001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430009">
        <property type="protein sequence ID" value="CAZ80109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835952.1">
        <property type="nucleotide sequence ID" value="XM_002835906.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6G6"/>
    <dbReference type="STRING" id="656061.D5G6G6"/>
    <dbReference type="GeneID" id="9181194"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004473001"/>
    <dbReference type="HOGENOM" id="CLU_1321740_0_0_1"/>
    <dbReference type="InParanoid" id="D5G6G6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39219">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39219:SF1">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003071997" evidence="1">
        <location>
            <begin position="27"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="208" mass="23389" checksum="ABEB5763A3CC1AEC" modified="2010-06-15" version="1">MRTLIHLLSLTFSLTLLLSHLTPSTAYRDSAPSKSEQDDNYSLTQELYQWPISAESPTPLCELWYHERWEDASCPSVGHKNCAMTGECGDDEIVRVGIMDEGGVFRGVVTTGKALKNRDKTLINIRRDRNNPTKVWRVEFQMVYDLEERDVRATNPTPGPQPFLNKPIVLNAEGKLPEPNVEKTFLQKYWWVLLAGALLVLSGGGGAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6R7</accession>
    <name>D5G6R7_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein mS33</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00002236001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mS33 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430014">
        <property type="protein sequence ID" value="CAZ80210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836053.1">
        <property type="nucleotide sequence ID" value="XM_002836007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6R7"/>
    <dbReference type="FunCoup" id="D5G6R7">
        <property type="interactions" value="172"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G6R7"/>
    <dbReference type="GeneID" id="9187235"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002236001"/>
    <dbReference type="eggNOG" id="KOG4844">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_150777_0_1_1"/>
    <dbReference type="InParanoid" id="D5G6R7"/>
    <dbReference type="OMA" id="MKAQCQV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013219">
        <property type="entry name" value="Ribosomal_mS33"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13362">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN S33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13362:SF2">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08293">
        <property type="entry name" value="MRP-S33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="98"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ80210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="132" mass="15238" checksum="1B3D40EAB75B8FB4" modified="2010-06-15" version="1">MATTIPTIVKLLPTKALPPRERLQELAKTSARIFSTTYNPHNLRTGNRVLRERLKGPTLLDYYFPGRRVGVVDLRRYFPMMSFADEDERQRLLDVQSKKLRGKGAPKKKKEANKKKAKIVRGSVEEYVLARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>D5G701</accession>
    <name>D5G701_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00004527001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430020">
        <property type="protein sequence ID" value="CAZ80294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836103.1">
        <property type="nucleotide sequence ID" value="XM_002836057.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G701"/>
    <dbReference type="GeneID" id="9182485"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004527001"/>
    <dbReference type="HOGENOM" id="CLU_1723653_0_0_1"/>
    <dbReference type="InParanoid" id="D5G701"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="152" mass="18047" checksum="47824992F054F238" modified="2010-06-15" version="1">MSTRKRKQEAAAEEGEEEEELQALPSDESEEEEEYVDSGPEDSGANWRRAMRMRRKLMMLRGRKMNPKTRREKGLRQKLKPNRKLRRKRLRTRTRRKRNNPVFPTSTFSLRSIDVRDIIIVHMYFHSTIFCSFSQKNIFGKGKGKTKKAESN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>D5G766</accession>
    <name>D5G766_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="14">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00002485001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ERG28 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430022">
        <property type="protein sequence ID" value="CAZ80359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836168.1">
        <property type="nucleotide sequence ID" value="XM_002836122.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G766"/>
    <dbReference type="FunCoup" id="D5G766">
        <property type="interactions" value="220"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G766"/>
    <dbReference type="GeneID" id="9185257"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002485001"/>
    <dbReference type="eggNOG" id="KOG3455">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114589_0_0_1"/>
    <dbReference type="InParanoid" id="D5G766"/>
    <dbReference type="OMA" id="NIAIWTY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016126">
        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005352">
        <property type="entry name" value="Erg28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15451:SF19">
        <property type="entry name" value="ERGOSTEROL BIOSYNTHETIC PROTEIN 28 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15451">
        <property type="entry name" value="ERGOSTEROL BIOSYNTHETIC PROTEIN 28-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03694">
        <property type="entry name" value="Erg28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="8" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="11" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ80359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="134" mass="14933" checksum="AA265D6BE550812B" modified="2010-06-15" version="1">MDYLTSCLPQTPGLLPKWLLFVAVVALGNSIQAYLTLRYNKRIYCTRPHEVTALSSRTFGTWTVLSAILRIHAAYYIQDPIVYDLAIWSYVVAGAHFVSEWLVFGSAGLNAGLMGPLLVSSTSLAWMITQRGEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>D5G799</accession>
    <name>D5G799_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00002406001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430023">
        <property type="protein sequence ID" value="CAZ80392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836201.1">
        <property type="nucleotide sequence ID" value="XM_002836155.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G799"/>
    <dbReference type="GeneID" id="9185319"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002406001"/>
    <dbReference type="eggNOG" id="ENOG502S0NK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039214_1_0_1"/>
    <dbReference type="InParanoid" id="D5G799"/>
    <dbReference type="OMA" id="TSWIMCT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004812">
        <property type="term" value="F:aminoacyl-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006418">
        <property type="term" value="P:tRNA aminoacylation for protein translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ80392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="526" mass="59268" checksum="C8EE4C2DDF325A70" modified="2010-06-15" version="1">MNLPTEAVFDARSEEEGSAIRAEDFETLEWRTGASMARISQVMPRAGTNVPTSVHPEILNGIVSTTATVQAALPPLDGSFDHYHYALHDGNVHNPLGAGSYIYKKEYISSHIRYLGLPLPTTGVDKCPRRFLPPSTLIIHIGAQPNNSPHIGTIVVFILAFLIARDVKKYYADLRRKADLSTEFAVWIGNFEVVVRLDIVDTAPHNQEGCVRDGINYQRSQRFTKAYHRLLPDYEEVMESAAAFVGGGIPYKLTNQETVASMPSVPRIIKAIVRDRERLGPELAPETGTLAIRAACPHERCGLAEKHGRKNKYSITPSGSTIIEFYCPNHGYHSVDSSNPKEVARLEFNTPLRNLVRTFAYGIQTTESRESARKAVAHRGTPTRELIHMRVTGSDYAGLYAEQLLWRQLILLKPEISGLTIQPPVIAYAPLIQDWAGSKLSKSLYVDKGAYGYLRATRMGYMLSFDEMKRQGKDHMILFREVEKWLEDPRKLFRPYSIDYLHRIFEQEGAREEAINKMGERRAVVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7S3</accession>
    <name>D5G7S3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="12">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00004702001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430031">
        <property type="protein sequence ID" value="CAZ80566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836375.1">
        <property type="nucleotide sequence ID" value="XM_002836329.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7S3"/>
    <dbReference type="GeneID" id="9181385"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004702001"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1034515_0_0_1"/>
    <dbReference type="InParanoid" id="D5G7S3"/>
    <dbReference type="OMA" id="SHKEAHH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000060">
        <property type="entry name" value="zinc finger protein 436"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000176">
        <property type="entry name" value="zinc finger protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF49">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF49.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="211"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="239"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="16"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="91"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="144"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="164"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ80566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="265" mass="29254" checksum="DC278A6F99B90B1A" modified="2010-06-15" version="1">MNLPSLVHQSRHHNHYPQSAQSNGLDFSDHQSPAPYLHRPHEYAHPPAPPPSPPVEDSNKCSLPSISTLLVFADGGQPGGNGMDRMERQQRSPQYQSQPLHSSGPTLPPTPPMRPDSVFEGNNSPTLSSTSPSSSYFYSPTSSQGGNRNSYPPAQSKYPSPPETSYLPQYSYSPSTPQSQPAMAYTSNPWHHHHHSSPASHAAFPQSQDRYICPTCNKAFSRPSSLRIHSHSHTGEKPFKCPHHGCGKAFSVRSNMKRHERGCHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8D2</accession>
    <name>D5G8D2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00004756001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430042">
        <property type="protein sequence ID" value="CAZ80775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836584.1">
        <property type="nucleotide sequence ID" value="XM_002836538.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8D2"/>
    <dbReference type="GeneID" id="9185995"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004756001"/>
    <dbReference type="HOGENOM" id="CLU_1714647_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8D2"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ80775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="153" mass="17157" checksum="AD3AC2677BAD529F" modified="2010-06-15" version="1">MSRYPSVWEHTSNILITLTYSNNDSLSKMSLSQICMVSYIILYKNPFCICPPHHPLPEPKPYYSHRNASTLSRFPAQSFPTVPLREENHFFATASCGRRSVGAGVGALFLYSLLERKYSLTGRDLHSSCLGLFAYLGATPCVRHFSIPSSFSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8P6</accession>
    <name>D5G8P6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00003069001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430049">
        <property type="protein sequence ID" value="CAZ80889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836698.1">
        <property type="nucleotide sequence ID" value="XM_002836652.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8P6"/>
    <dbReference type="STRING" id="656061.D5G8P6"/>
    <dbReference type="GeneID" id="9186256"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003069001"/>
    <dbReference type="eggNOG" id="KOG2348">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023225_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8P6"/>
    <dbReference type="OMA" id="NIWYIRA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015606">
        <property type="term" value="F:spermidine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050277">
        <property type="entry name" value="Sodium:Solute_Symporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48086:SF10">
        <property type="entry name" value="AGR155CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48086">
        <property type="entry name" value="SODIUM/PROLINE SYMPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="179"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="210"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="249"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="280"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="328"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="369"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="395"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="428"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="468"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ80889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="541" mass="58477" checksum="8FCE52BB044843F1" modified="2010-06-15" version="1">MGMPPQPVSNGLMYTTYAIFLIIGLALAWRYRGQSKLEFIHSNRTQTAIPVALNFIASGECCYRRNGLRHVIGQLGSSAMGSAILFSYPEIGTIAGVQGAITYAACSALPLMSFAILAPKVRRKLPGGFILTMWVRERYGPYAALYLTFLSLATMFLYMVAELSSLQQVISTLTGLNGLPVVIVEVVVTSIYTSLGGFRISFLTDNIQGVLIAVLIAICTIAIGTSVEIDRSRIGPSGLTHDTLLGYQLIYILFTGILFSFMFLSSFWMRAFASKTDKDLWIGVSIAAVVIFVILLLVSSTGFIAAWAGVWEPGMYGGMAFFLLLAELPSWVVGFTVVMVVALSCAIFDSQQSAMVSTISNDLFKNKLSLIWVRFFVLLVSIPAIVVALKSPSILRIFLISNIFSCAAMPSILLGLLDTMYFLRGVDIIIAGVGGMLSVFLFGCAYYGDAKKAAELIILTTGLYANDWSVFGVFVAAPVGSLIFLALAVACRMGGVWVRCKVQGGRFDCLDKPLPREGGVCEGDGTPGGSLEDVERRKVVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8S8</accession>
    <name>D5G8S8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00004821001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430053">
        <property type="protein sequence ID" value="CAZ80921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836730.1">
        <property type="nucleotide sequence ID" value="XM_002836684.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8S8"/>
    <dbReference type="GeneID" id="9184323"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004821001"/>
    <dbReference type="HOGENOM" id="CLU_1846563_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8S8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ80921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="139" mass="15503" checksum="51DEC124996BF5F6" modified="2010-06-15" version="1">MGHSAYSVAAALPGRKGEEEGAETKTYRTLKSPKNDRALRPRICRIKLLCVLSVRSSGVQYRYCPPAPVGKSIVRCRFHHSFTRGILLCQLRLHHSHIRCEDGLYEEVGKKKSTYPLGPMIAKGVPRRIVGAEYGPCIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8U9</accession>
    <name>D5G8U9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">gamma-glutamylcyclotransferase</fullName>
            <ecNumber evidence="1">4.3.2.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00004849001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.3.2.9" evidence="1"/>
    <dbReference type="EMBL" id="FN430053">
        <property type="protein sequence ID" value="CAZ80942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836751.1">
        <property type="nucleotide sequence ID" value="XM_002836705.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8U9"/>
    <dbReference type="GeneID" id="9186451"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004849001"/>
    <dbReference type="eggNOG" id="ENOG502S3WQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048475_1_2_1"/>
    <dbReference type="InParanoid" id="D5G8U9"/>
    <dbReference type="OMA" id="EVMRKSI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003839">
        <property type="term" value="F:gamma-glutamylcyclotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd06661">
        <property type="entry name" value="GGCT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.490.10">
        <property type="entry name" value="Gamma-glutamyl cyclotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009288">
        <property type="entry name" value="AIG2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017939">
        <property type="entry name" value="G-Glutamylcylcotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013024">
        <property type="entry name" value="GGCT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036568">
        <property type="entry name" value="GGCT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12935">
        <property type="entry name" value="GAMMA-GLUTAMYLCYCLOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12935:SF0">
        <property type="entry name" value="GAMMA-GLUTAMYLCYCLOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06094">
        <property type="entry name" value="GGACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110857">
        <property type="entry name" value="Gamma-glutamyl cyclotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma-glutamylcyclotransferase AIG2-like" evidence="5">
        <location>
            <begin position="12"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="12"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR617939-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR617939-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF06094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ80942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="206" mass="23445" checksum="BE61F127A29CA417" modified="2010-06-15" version="1">MTTTDTKPPTYYFAYGSNLWLSQMHRRCPGHKLIGTALLPCHRWHINTRGYANLVPTTTTNNHHHHDTYGVVYTLSESDEAQLDLSEGVPYWYQRVKHPVKLLPSSGDGGDGEGEGERMLEDVLVYIDTIRTTDGSPKEEYIHRINNGFRDAVGIPKEWVERYVRKYIPAGVEDMVEDPFLFKSEGEVESAVEKEASRGGYCPRLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8W7</accession>
    <name>D5G8W7_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">COP9 signalosome complex subunit 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00004873001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the peptidase M67A family. CSN6 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430053">
        <property type="protein sequence ID" value="CAZ80960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836769.1">
        <property type="nucleotide sequence ID" value="XM_002836723.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8W7"/>
    <dbReference type="STRING" id="656061.D5G8W7"/>
    <dbReference type="GeneID" id="9181243"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004873001"/>
    <dbReference type="eggNOG" id="KOG3050">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027018_2_0_1"/>
    <dbReference type="InParanoid" id="D5G8W7"/>
    <dbReference type="OMA" id="LVGWWST"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008180">
        <property type="term" value="C:COP9 signalosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000338">
        <property type="term" value="P:protein deneddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08063">
        <property type="entry name" value="MPN_CSN6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024969">
        <property type="entry name" value="EIF3F/CSN6-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033859">
        <property type="entry name" value="MPN_CSN6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10540:SF8">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10540">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01398">
        <property type="entry name" value="JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13012">
        <property type="entry name" value="MitMem_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0736">Signalosome</keyword>
    <feature type="domain" description="MPN" evidence="4">
        <location>
            <begin position="21"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="339"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="358"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU367006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ80960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="383" mass="41322" checksum="9729ABC18785F724" modified="2010-06-15" version="1">MAEISDDSLISTHPSTSGLQIALHPLALLTISDYITRHTLRKQTGPMVGALLGTQEGRDIAIEHAYEIITLVDKDQVRIDEEWFDNKLKLFKETHPTLDLLGWFTTTTSPTFAPSLYMVPIHQKMLNYNESAIILCLNPAPNASAGGGGKLPLGIYESIIETEDAAAAGSVESMLKLRFVPLKYTIETGEAEMIGVDFVAKGGFGNATAESSGLEGTAEGKLNVGTQNDELLANLTAKANAIRMLHSRIRLLTNYLSDPPHKKPNHQLLRALKSLTHSRLPLLTPADAGAFRQEQLAEQSDVHLVALLGVLTRSIEEARGVGKKFAGIEATNKANMRMGGSGAAGEPAVWENYGPPKDPTRRGRRENSSGGGSAPFGNYPSNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9H2</accession>
    <name>D5G9H2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00003400001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430061">
        <property type="protein sequence ID" value="CAZ81165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836974.1">
        <property type="nucleotide sequence ID" value="XM_002836928.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9H2"/>
    <dbReference type="GeneID" id="9182916"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003400001"/>
    <dbReference type="HOGENOM" id="CLU_367689_0_0_1"/>
    <dbReference type="InParanoid" id="D5G9H2"/>
    <dbReference type="OMA" id="RWHERET"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008626">
        <property type="entry name" value="Mediator_Med15_fun"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05397">
        <property type="entry name" value="Med15_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="340"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="455"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="537"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="637"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="82"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="138"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="346"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="359"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="459"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="542"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="758" mass="84061" checksum="B291220B08543FAF" modified="2010-06-15" version="1">MSSNQLTQLQKLASQGRLDPDDQRQLQQHIFRLRELHQMRQIQRARAAHPQNVGPNQPAQQPPRPPAPPQTPITRPQPPQQQPQQSQQLHHQQQQPGSGDSPSALKKTKRERDQSEDDLVVIDRKQAVQQPQRPGPDRPQGQQPTGPSQFSNQVQQQAQKTNAPQQAPMLPGNAAQAGQAISEATAAKQAVMEKEQQGQNLNQRMQINGLMKLLEEEKNSHESRLPLQLDANERGFLRQQLGDESTKNMIRRTDQLLPIFIMLNGPEKLTRELIRTKNLLASQYHPTGDPKDVFTLSLREFTESRGSLTKYFSYVKTNMQNAFNKDPERAKEIQMRALAFANPNGGPFPGPRVPPASTPQQPQRVSPPQAQAQAQQHQQLQVPVPPSSENVLSTRNLEKQTNLLALERSRSLQSRESQLNARVQAVGSMSPDVCTPIDIKPRISVDDLKFPPARKKRLREPDTVEPPKAKPLGEQKTFKCPQPGCEIGGKGFVTNRELLEHQRWHERETQKAIEEAEKRERMRKDPVGYFLSSVREGLGLGEDGKPKEKKDESVAAMKDDTPSRTACATPQVKAGSTPLLPNTPLNMKSGFTPLPSGKSPALPAFCTPHPTNAKTPGSAGRSTSGIKTMTKEDHQLQHHNDQIPTPPGSNAWEGASMSPDVLRQCFDGLSEQTSGLSALYPPIFTPTYTPSPSDAEGGDSMSENVGGGYEDWNPFGYKGSLGDEMLQEIEWDNQPASTNMGKDWALESGYRMNASVLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>D5GA91</accession>
    <name>D5GA91_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mitochondrial import inner membrane translocase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00005187001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Mitochondrion inner membrane</location>
            <topology evidence="1 8">Peripheral membrane protein</topology>
            <orientation evidence="1 8">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the small Tim family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430075">
        <property type="protein sequence ID" value="CAZ81445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837254.1">
        <property type="nucleotide sequence ID" value="XM_002837208.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GA91"/>
    <dbReference type="FunCoup" id="D5GA91">
        <property type="interactions" value="472"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GA91"/>
    <dbReference type="GeneID" id="9185727"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005187001"/>
    <dbReference type="eggNOG" id="KOG3489">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_141397_1_0_1"/>
    <dbReference type="InParanoid" id="D5GA91"/>
    <dbReference type="OMA" id="NEICWDK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.810">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit tim13 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004217">
        <property type="entry name" value="Tim10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035427">
        <property type="entry name" value="Tim10-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02953">
        <property type="entry name" value="zf-Tim10_DDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144122">
        <property type="entry name" value="Tim10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="4 8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0811">Translocation</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Tim10-like" evidence="9">
        <location>
            <begin position="24"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ81445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="92" mass="10416" checksum="9B2D95E5D82FF7AD" modified="2010-06-15" version="1">MDNSTPNPDFSDFSDKDKKDLQSFIENEQQKAKFQANVHNLTDLCWTKCITGKISGATVDRNENSCLENCVNRFIDSQKTIVRQLDVMGGRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAC7</accession>
    <name>D5GAC7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00005238001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430075">
        <property type="protein sequence ID" value="CAZ81481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837290.1">
        <property type="nucleotide sequence ID" value="XM_002837244.1"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GAC7"/>
    <dbReference type="GeneID" id="9185763"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005238001"/>
    <dbReference type="eggNOG" id="KOG1860">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_245544_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAC7"/>
    <dbReference type="OMA" id="TWIAQPP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070390">
        <property type="term" value="C:transcription export complex 2"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045107">
        <property type="entry name" value="SAC3/GANP/THP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005062">
        <property type="entry name" value="SAC3/GANP/THP3_conserved"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12436">
        <property type="entry name" value="80 KDA MCM3-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12436:SF3">
        <property type="entry name" value="GERMINAL-CENTER ASSOCIATED NUCLEAR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03399">
        <property type="entry name" value="SAC3_GANP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAC3/GANP/THP3 conserved" evidence="2">
        <location>
            <begin position="292"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="672"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="727"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="796"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="899"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1436"/>
            <end position="1470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="83"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="172"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="214"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="225"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="677"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="796"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="806"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="822"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="838"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="850"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="872"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="907"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="942"/>
            <end position="970"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ81481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1569" mass="171113" checksum="7BFB4ACB4A491B71" modified="2010-06-15" version="1">MPRAFGGSGNATLDAPGSTPGRTNGRENHSPRFNTSHSSRGNRLPSRHRQRNSAQQNRSSQQNDSSSARNSGFPSHAGEPREQQNQQQTRQPNLRPVSQRYVSPNGNAQEGVGLSSYITGRMEPNGRPGTARRGRGDVSSRGRGKGAFGAASGGPPLGGNPFGGPSSKPTPGRGGRGSRGGVRGGRGGYQRGSPAPSGGDESDVSGDEGAVAQFTDSAGSFNGENPRDKDAAKSARAERFSDKAPTMALFERMKNDRVRERRDAIANGTIDDPDVPKRLEDALDFVGTCMDMCPEFERVERSVQHSVDPLELDPRTGEIDRNYAVKRFHRPAAGNDAQLPSDVRPPKVLISTLAYLVYNLCGGDIPLSKTHPFVRDRTRAIRQDFTLQNYRKAETVQCHEIIARFHILSLHKLAKDTPDHFVAQQEIEQLQKTLTTLMELYEDARLDGYNYVNEAEFRSYQIITHIRDPDLQRQAQRWPSNIFSSAPVQMALKFFALIQANNRKQTNLGTKNTESCFNNFGTFFRLVKSERVPYLMACLLETHFSEVRKYALKAMRGVYRRDSKQILLKFVQETLAYEDEASLMADCDNYGLSYAMTDPDPGQEPESYLVIHKGKGPWTDNLPTKQSFHEFVEAKLMGRSISDVLWEPKEGAMSNETKAQLLLSRAPHQRTLDPSALTPQVSPFPPTSQNGFGSQPLPKLSNLSSKPAFGPSTLSAAAQVALGSAPISKPLHPDGIRSFGSRGSVPDSEIAPGFPGPQPVAAMNNVFGGVQSNSSVFGNVPREFPAVGSLTAGTSPFAASSTGPPQKNPFGPPAVGPLKFATGTSSVPTSTSSPVGFNSVTNESATLNPQFPPQLKPAPLGDPPAPVQPPFGSVAVSPGSPAAPSATASVFQPIADRKQPLFPPVAPQQSAQSSALGNSLGSRPSGSAPLPSLFQKPETPSLFDSNSVPASANGGSVFDSLQNSSRSSPASFVFPPYVPDDTGDDDELADNVATQSYTHSSDLVNNYPPATSMSSQAWPAPTGDIDQGSLFASRLSGLKQNSAVPSISSLAQPFSAAAPEPPHPVPTNKKLFHSPARITELADSLFDQLLEGKIKEVVQNVMEDSRVALAESIASDFLDIQASKIAANVVQEAIAESFHETRLKEKVFVPWKRVSRKLWLKRLRQERIKNPPPPISPQWHPVQFPDNGAPSVEVHKYINLMFKAPPLADLFHPKVENAFWATSRKGEHPTWRLLIVNTNSEDAGNFWWKNKFLGQGMPRKAISDRATFEAQLKLEQGDMGQVKETGGFIFGCSASYNVSNEERFKSDRRNLHDAVEFMLECTSFQKLAIVIVCYRSPLDTTDADPFGESDRTSGEGREKRLAAIREALALNEFGSRVIGKEIILVETFDDVDFTPAMKRLSGLVLESVNPAVAEALAPKKSLKRKRILHPPMFANLVVPPPLDQTPKNEKSGGSARPKRRKSTPPDRHIITGSMQRGYIDYQTQKLGWRKSAGGALPNGVKDPVREEILVTTRTPIQPTSESKKSSPIASLSAEVEDAMNITRGLDRSFDDITEDFRELAYRWLRKESR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAK5</accession>
    <name>D5GAK5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00003625001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430078">
        <property type="protein sequence ID" value="CAZ81548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837357.1">
        <property type="nucleotide sequence ID" value="XM_002837311.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAK5"/>
    <dbReference type="GeneID" id="9187488"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003625001"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_584210_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAK5"/>
    <dbReference type="OMA" id="FAGCKKS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000125">
        <property type="entry name" value="Putative zinc finger protein 143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000072">
        <property type="entry name" value="zinc finger protein 143 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF178">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="22"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="52"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="82"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="112"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="135"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="390"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="144"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="171"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="181"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="207"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="390"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ81548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="468" mass="51705" checksum="E7E7C208C5F7DAA3" modified="2010-06-15" version="1">MDILELIDDREQTVAESSPRPFCCPEQSCNKSFNRKSDLQRHHRIHTNERPYSCTFPNCGKSFIQRSALTVHTRTHTGEKPHSCEYIGCGKCFSDSSSLARHRRIHTGKRPYSCPIDKCGKSFCRKTTLTKHARKNHQLRAENDVSDDDVEESDDDDLSPKASGKAGSRAQSKRLTKASARRAAPPSAPSRPSVLRTSSFAEPLTPHSPQSTVRSGHSSRNTSFSAASDSYRHSMPMAMTHSVHQHQPPTPQSPYYTEEDVGDGREISPNAIIHRDDEYGTTPTGPPPPLQGNRSFDTLSIVCSTPTTHQLLAAQQTLQSSPGSLSSCSSATTASCGSDYFYRAPQPTSSGHYQNMGQAISPGGIPPYPAQMPVHTGPSQHPIVMYSQNGQHQSNGILSPHSQAQAQQQQQQQPVWYDYPAYQQQILAAQTQPPQHRIYYTGVPVQDPNFIKTEQEQNLLPTPRGSFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAS2</accession>
    <name>D5GAS2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="13">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00005276001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430086">
        <property type="protein sequence ID" value="CAZ81615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837424.1">
        <property type="nucleotide sequence ID" value="XM_002837378.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAS2"/>
    <dbReference type="STRING" id="656061.D5GAS2"/>
    <dbReference type="GeneID" id="9187782"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005276001"/>
    <dbReference type="eggNOG" id="KOG0769">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015166_6_3_1"/>
    <dbReference type="InParanoid" id="D5GAS2"/>
    <dbReference type="OMA" id="QFMIIES"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015217">
        <property type="term" value="F:ADP transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080122">
        <property type="term" value="F:AMP transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005347">
        <property type="term" value="F:ATP transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015228">
        <property type="term" value="F:coenzyme A transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015230">
        <property type="term" value="F:FAD transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044610">
        <property type="term" value="F:FMN transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051724">
        <property type="term" value="F:NAD transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000119">
        <property type="entry name" value="Mitochondrial carrier domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052217">
        <property type="entry name" value="Mito/Peroxisomal_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45939:SF5">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PMP34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45939">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PMP34-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="9" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="190"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="251"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="6"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="189"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ81615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="288" mass="31390" checksum="E576576EA97E238B" modified="2010-06-15" version="1">MSSAQSDNVLHAIAGAGGGVLSMVLTYPLITLSTRSQVESKRAHSTVLDATRRIIQREGISGLYAGLDSAVFGISVTNFVYYYWYEWSRTAFEKAALKAGRSRAKLTTLESMAAGALAGSATVLITNPIWVVNTRMTARKRAAAPSSSPHRPLHYLHYPRGWSVLCGGNSRQLKNMLERRKKRSVTPQDAFFLGALGKLVATALTYPYITVKSRMHVADKGEKKHSVFESLRNIVNEEGWTGLYGGIGPKLVQSVLTAAFLFAFKDALYAFTATTLANRRAAAKLKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GB04</accession>
    <name>D5GB04_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00005386001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430086">
        <property type="protein sequence ID" value="CAZ81697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837506.1">
        <property type="nucleotide sequence ID" value="XM_002837460.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GB04"/>
    <dbReference type="STRING" id="656061.D5GB04"/>
    <dbReference type="GeneID" id="9187865"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005386001"/>
    <dbReference type="eggNOG" id="KOG3085">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045011_1_0_1"/>
    <dbReference type="InParanoid" id="D5GB04"/>
    <dbReference type="OMA" id="TAPNGFW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02603">
        <property type="entry name" value="HAD_sEH-N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="entry name" value="Putative phosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052898">
        <property type="entry name" value="ACAD10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47829:SF1">
        <property type="entry name" value="HAD FAMILY PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47829">
        <property type="entry name" value="HYDROLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G12880)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00413">
        <property type="entry name" value="HADHALOGNASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ81697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="248" mass="27283" checksum="DA51C575A708A9B8" modified="2010-06-15" version="1">MVIKACIFDIGGVCVLSPLHAIRAYETENSIPSGYISYAIVASSPSGSWDRLERGEIPMDSTFYTGLTSDLTSPETWSSFLRSRNLPSGAASPLPHIDGETLFRRMMTESRAPNPPVIAAIGKLRATGRYKLAALTNDYQFPSGHPFADNLGLRGLFDVFVSSNECRMRKPERGIYRLVLERLNVAPGEAVFLDDLGLNLKAARELGIITVRVPLEETWRAVRELEGVLGEKLLDEEDEDLAGGKARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>D5GB28</accession>
    <name>D5GB28_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00005414001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430086">
        <property type="protein sequence ID" value="CAZ81721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837530.1">
        <property type="nucleotide sequence ID" value="XM_002837484.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GB28"/>
    <dbReference type="GeneID" id="9187962"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005414001"/>
    <dbReference type="HOGENOM" id="CLU_845167_0_0_1"/>
    <dbReference type="InParanoid" id="D5GB28"/>
    <dbReference type="OMA" id="PERMMEV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="305"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="329" mass="37940" checksum="A467FB8DD30A6C2A" modified="2010-06-15" version="1">MASLEFLATELLLNIVQYVETPEEFTTVDGSKEQPSLKNLSSTCRRLRAVCFPFLFRSVKLPLEKGHTCKSIEELRSFILNNALEKTIESIGLAMGSQWNEGKVGLELKKFLWEISVKSLSIRQYRTHHTLREKSARWPYFLATLERLRLEEVDDDNDLKILLLKQCPIRLLHVNDGNFRSLYEEDDKYFLGRAESGPGITSFKPSAFPNLTTVVYHAAWPPYNRFDGFLEFVSKLPAIKVLEVKLMGDEDLELDLKRKSYKLPPDAIRFIEVVSAYQILAWRVGTIDSLQRLVIRDHRIPYHGETDPPQSFAEGPVGTYQRQNDTRTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>D5GB87</accession>
    <name>D5GB87_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">DNA damage-binding protein CMR1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00003793001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">DNA-binding protein that binds to both single- and double-stranded DNA. Binds preferentially to UV-damaged DNA. May be involved in DNA-metabolic processes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the WD repeat DDB2/WDR76 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430087">
        <property type="protein sequence ID" value="CAZ81780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837589.1">
        <property type="nucleotide sequence ID" value="XM_002837543.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GB87"/>
    <dbReference type="FunCoup" id="D5GB87">
        <property type="interactions" value="623"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GB87"/>
    <dbReference type="GeneID" id="9181490"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003793001"/>
    <dbReference type="eggNOG" id="KOG4328">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017019_1_1_1"/>
    <dbReference type="InParanoid" id="D5GB87"/>
    <dbReference type="OMA" id="GKYHPSS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000001">
        <property type="term" value="P:regulation of DNA damage checkpoint"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050853">
        <property type="entry name" value="WD_repeat_DNA-damage-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14773">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14773:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 8 9" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="279"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="32"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ81780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="443" mass="49462" checksum="0F0AA6A99F86BD40" modified="2010-06-15" version="1">MEDSNEYERLRQENILKNKELLLRLRLDAASVGTTKRAAISSSGSASKPKNRSVKKELQSPLPTRKSSRLAGLPADSEVAKRKYEEGVAALQEAEKAKRARVAGDLSFEIRQGLIDVTKSARFERTFTDENVKETTDKDLRAMRQEMMGLRLYDQHLPNGKLTKEAKNLQNLQTPPPQRIPPLTISDLDGNPRQYKVHSRTISTFTFDPISATSLYTSSYDGSVRKFDLATGVASEVFVAEDGDALSGVEVHDTNILYFSTLDGLFGRRDLRQRETDIWTLHEKKIGGFSTHPRAPHLVATGSLDRTLKIWDLRKVVKTSEYRTLALVAEHPSRLSVSCALWSSTGSLATTSYDDTIKIYKFPDAVRWVTILRAQWQQAPPNGQQKLVIANMSRFLDVYSENGEQLAQLSHESVTTVPAAAQFHPTRDWIAGGTASGKVVLFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBC5</accession>
    <name>D5GBC5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00000422001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cell cortex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the synembryn family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430091">
        <property type="protein sequence ID" value="CAZ81818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837627.1">
        <property type="nucleotide sequence ID" value="XM_002837581.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBC5"/>
    <dbReference type="GeneID" id="9184397"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000422001"/>
    <dbReference type="eggNOG" id="KOG4464">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015532_0_0_1"/>
    <dbReference type="InParanoid" id="D5GBC5"/>
    <dbReference type="OMA" id="NADPIFT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001965">
        <property type="term" value="F:G-protein alpha-subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008376">
        <property type="entry name" value="Chaperone_Ric-8_A/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019318">
        <property type="entry name" value="Gua_nucleotide_exch_fac_Ric8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12425">
        <property type="entry name" value="SYNEMBRYN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12425:SF5">
        <property type="entry name" value="SYNEMBRYN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10165">
        <property type="entry name" value="Ric8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01802">
        <property type="entry name" value="SYNEMBRYN"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="401"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ81818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="463" mass="51827" checksum="803C7509A7BCDF7C" modified="2010-06-15" version="1">MAAPSSNASKLSQVQALVDMLRTDLQDHHLPPEKRVITLEKLKVFGRDVNQSDPIFTMEAKMLCKHAFEGTDSKSAQEAMRCLANALLKVPGTRQIFVELGYTGQAVNRYQNDSLDEEFLAGRILFLVTYATAETPKWLEEYKLANYLNDAITRHGIIYSKINKNEQPSALQDMAFTETTKLLFNLTHFAPEKVDLFSRSIPTLFKILCRRTLPSLPLQSSVAHLVNALLNLDLSSQAGCVFPSFDPTCNVARLIEVLDLSVESPNDNRNNQRNQLFNEAGAPLVTLLRKVFEISSDQAKKYMRIRLLPSDEERKNPRGKDDSLASRLLQLSTSAAALNIRQHLASMLFEVSNSNPEEFVRNVGYGYASGLLLSAGVELPQSALESLSASSDSSGRLFNPITGQALDSEPETKDPFSEMTDEEKEREAERLFILFERLKQTGVVDIQNPVEKALHEGRFEELD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBC8</accession>
    <name>D5GBC8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00000425001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430091">
        <property type="protein sequence ID" value="CAZ81821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837630.1">
        <property type="nucleotide sequence ID" value="XM_002837584.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBC8"/>
    <dbReference type="STRING" id="656061.D5GBC8"/>
    <dbReference type="GeneID" id="9184394"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000425001"/>
    <dbReference type="eggNOG" id="ENOG502S1SC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027663_1_1_1"/>
    <dbReference type="InParanoid" id="D5GBC8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="193"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="253"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="208"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="286"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ81821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="390" mass="43081" checksum="D3793587EAA3396A" modified="2010-06-15" version="1">MKLQRLSRALETQQAHQEGDPNPQGKALIIAVVLISFLFFAIITYIVLRYIRQAHPLAPKFFPEKWKKKWITWQPGIYAPTASSASPTQEPSAAARRELERRERRERRRREQRQQQQASGVNRVESVRSIMTLPEYRAVPLPDRERTIGREGERGGIDVVVEAPETAEEEEERREEHMRALYEIRLARQLERAAERESAQNGGRGGSATHTAAGSRNRGNGSATSLAATLAAVQERERRLSEVSYAEIGVARPDGSRVRASSDVSDRDSLPLLDAGAPMGVGDSIRSGQSSHIRTNSALSLTSTLGTPERRRSDEIFGMGSTRPSLDNRDSIHGSAPSDRPPGYSGDPDWGAPPEYPGAVEDGRGDMSVPALRIETASPDPYRSPQQAHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBK4</accession>
    <name>D5GBK4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00005675001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ82010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837819.1">
        <property type="nucleotide sequence ID" value="XM_002837773.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBK4"/>
    <dbReference type="STRING" id="656061.D5GBK4"/>
    <dbReference type="GeneID" id="9185445"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005675001"/>
    <dbReference type="eggNOG" id="KOG0946">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006318_4_0_1"/>
    <dbReference type="InParanoid" id="D5GBK4"/>
    <dbReference type="OMA" id="GQETFCN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005795">
        <property type="term" value="C:Golgi stack"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048211">
        <property type="term" value="P:Golgi vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048280">
        <property type="term" value="P:vesicle fusion with Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000296">
        <property type="entry name" value="Related to transport protein USO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024095">
        <property type="entry name" value="Vesicle_P115"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006953">
        <property type="entry name" value="Vesicle_Uso1_P115_head"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10013">
        <property type="entry name" value="GENERAL VESICULAR TRANSPORT FACTOR P115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10013:SF0">
        <property type="entry name" value="GENERAL VESICULAR TRANSPORT FACTOR P115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04869">
        <property type="entry name" value="Uso1_p115_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vesicle tethering protein Uso1/P115-like head" evidence="5">
        <location>
            <begin position="336"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="784"/>
            <end position="810"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ82010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1002" mass="111734" checksum="610307FCED10F382" modified="2010-06-15" version="1">MSLLGILQAQAPPKQDATSTIQTLCLRLSTSTLLEDRRAAIMGLRSFAREYKETVASGGLRGLISTLSKDSEDVDTIKVVLETLLLLFVKDESNSESSEDIALWLTDEFTQKQENITVLIELVQDSDFYIRLYALQLLVAILNNRPSRTQECILTAPLGISRLVSALDDKRDAIRNEGILLIINLSNGHTDIQKLIAFENAFERMFSIIELEGGVDGGIIVQDCLQLLNNLLSFNVSNQSYFRETGCVPRLAKLFQFAQEPVPSYAKGQSDTNIEYAMRVCRLFVVPGGLGAAANQNAFFGAGILHLMLVIAFSGFSDFPVRAEALRTVADLIHENSLLQENFAQMKVQYLDPTILPEVQAQQNNPDDLCYVIEGLLDLALLNSSIHAFDARLAACRCLESYFAGNQPVRLHFLNHAINLHAQGDESANVLTCLINLDQDSRGDPYRVWLASVIFLHLIYSDEEAKNLATSFKEGDADAGEEVVTVIQSISANLVAALEHNYDPRIIIGYLMLLCTWLFEDSPAVDDFLNEASSVQSLVSTVKQSSNSNVLIQGLCAFFLGILYEFSHKDSPLPRATLHPILASRMGRDHYVNKVTKLRENPQVRDFEVFRETNRRKRKIGLPEVYFDHTFIDFLKDNYSRVLRAIDKDPGLETHNTINGIPHALNGISVELVDSLKAQLEDKDEELQKLQTTCLTLEQKITQEQHESQRTKEAAAAEVQKANAATEALRLQYDVELRATAQKARSEINGLEGQIQRTRWEASEAARKAKEYGDSEVAGLRRRVAESEAATEKEKAERAGEVQRAKAEGEKAIEEVTSRLHSAEKEALFAHESLRKSEEHARESDQRLKTSEEALKAANERIASLEEGSRRQKSEATILQEALTAAQSRLEECTKQQAASEAQIKDLETTLRALEASLIKEQSTHVRILHEAQESAAALEVRVKELDARLSDAEHARKEADEAAKQSAAELDDLFIVLGDLEEKREGDKVFFPFAVCEIYSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBL5</accession>
    <name>D5GBL5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00005690001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ82021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837830.1">
        <property type="nucleotide sequence ID" value="XM_002837784.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBL5"/>
    <dbReference type="FunCoup" id="D5GBL5">
        <property type="interactions" value="123"/>
    </dbReference>
    <dbReference type="GeneID" id="9185456"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005690001"/>
    <dbReference type="eggNOG" id="ENOG502R27B">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033748_2_0_1"/>
    <dbReference type="InParanoid" id="D5GBL5"/>
    <dbReference type="OMA" id="QASVRSW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06897">
        <property type="entry name" value="PX_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15841">
        <property type="entry name" value="SNARE_Qc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22775">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22775:SF3">
        <property type="entry name" value="SORTING NEXIN-13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="2">
        <location>
            <begin position="2"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="1">
        <location>
            <begin position="316"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ82021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="368" mass="39930" checksum="7D28468C274ADAF3" modified="2010-06-15" version="1">MTTLEISIPTTSLATSPGKPHTVYHILLRLPLRSITVKKRYNDFVELHSRLTDTVGSVPPKPLPPKSYFSRTVGNPAMTEERRDGLEKYLLGILHSGDPRWRDSPAWRTFLNLPANWSAGSSAASSVSLVGYTPGIGGGGGPITDPTLWLDTHRELKTLLHDARMYLSKRDQAGSAGEQHEASAGAKRCLVKVGSLITALDNGLKEVGNGGRGNNGWGAGKEGKELLDGEIRRRKDLVSSARKERDGLESLANSLASKRAAQNSSVAAMAADKNALFTGASASKNTFGGGRVLGAPLPETERTRELDNRGVLQLNDQYMREQDRVVEGMLKNVGRMKEIGVAIGEEIDLQNKMLEDLDRDVDKPSAKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBN7</accession>
    <name>D5GBN7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00005495001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ81887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837696.1">
        <property type="nucleotide sequence ID" value="XM_002837650.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBN7"/>
    <dbReference type="FunCoup" id="D5GBN7">
        <property type="interactions" value="1052"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GBN7"/>
    <dbReference type="GeneID" id="9182924"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005495001"/>
    <dbReference type="eggNOG" id="KOG1092">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018687_4_0_1"/>
    <dbReference type="InParanoid" id="D5GBN7"/>
    <dbReference type="OMA" id="SCYNIFN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.750:FF:000007">
        <property type="entry name" value="TBC1 domain family member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000037">
        <property type="entry name" value="TBC1 domain family member 22A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000001">
        <property type="entry name" value="TBC1 domain family member 22B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.750">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF26">
        <property type="entry name" value="LD44506P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="2">
        <location>
            <begin position="172"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ81887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="479" mass="54643" checksum="875F5AFC98C4453D" modified="2010-06-15" version="1">MLKFESLSVQPKPPVASGGHTPSPPLPNGPVKNFSDYMNQTDDDWDADVGDETPDDFLQRKLKPPLSIEHQPAVGSKLGGPSSSTNLASKGPSYPSPRRKDNQGLKPQFEGLVRDPGNSLHMIYHPTLSLDTHTEAEIERLNARIKRINKFKRILQSSTVDLNELREISWNGIPDELRPMAWQMLLGYLPANSDRRVSTLERKRKEFIDGVKQAFVRGTSGLDQTIWHQISIDVPRTNPHLPLYGFETTQRCLERILYVWAIRHPASGYVQGINDLVTPFFQVFLSAYIDGEVETFDPSSLPKEVLDVIEADSFWCLTKLLDGIQDNYIHTQPGIQRQVTALRDLVQRIDVGLTKHLSEQGVEFIQFSFRWMNCMLMREVSVKNTIRMWDTYMAEGQSGFSEFHLYVCAAFLVKWSEQLQKMDFQNVMMFLQSLPTQSWGEKEMEILLSEAFLWQSLFRNSSAHLRQPTSNASLPSITL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBS2</accession>
    <name>D5GBS2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="11">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00005552001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="9">Binds 1 [2Fe-2S] cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I 24 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ81922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837731.1">
        <property type="nucleotide sequence ID" value="XM_002837685.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBS2"/>
    <dbReference type="STRING" id="656061.D5GBS2"/>
    <dbReference type="GeneID" id="9182959"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005552001"/>
    <dbReference type="eggNOG" id="KOG3196">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054362_1_0_1"/>
    <dbReference type="InParanoid" id="D5GBS2"/>
    <dbReference type="OMA" id="ERTMHYL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902494">
        <property type="term" value="C:catalytic complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098796">
        <property type="term" value="C:membrane protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006120">
        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03064">
        <property type="entry name" value="TRX_Fd_NuoE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000022">
        <property type="entry name" value="NADH dehydrogenase flavoprotein 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1590:FF:000001">
        <property type="entry name" value="NADH-quinone oxidoreductase subunit E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1590">
        <property type="entry name" value="NADH-quinone oxidoreductase subunit E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002023">
        <property type="entry name" value="NuoE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042128">
        <property type="entry name" value="NuoE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041921">
        <property type="entry name" value="NuoE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01958">
        <property type="entry name" value="nuoE_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005725">
        <property type="entry name" value="PRK07539.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10371:SF3">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] FLAVOPROTEIN 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10371">
        <property type="entry name" value="NADH DEHYDROGENASE UBIQUINONE FLAVOPROTEIN 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01257">
        <property type="entry name" value="2Fe-2S_thioredx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01099">
        <property type="entry name" value="COMPLEX1_24K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="5 9" id="KW-0408">Iron</keyword>
    <keyword evidence="6 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-1278">Translocase</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000216-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ81922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="263" mass="28799" checksum="C08D361D9198D764" modified="2010-06-15" version="1">MSQRRSSSCMNTPQNDPSIPFSFTPQNQQLIDEILKRYPPQYQKAAVMPLLDLGQRQHGFASISVMNEVARILEMPPMRVYEVATFYTMYNRDPVGKYHLQVCTTTPCMLCDSDSVMQAVEKFLGVKPGNTTQDGIFTFSEVECLGACVNGPMVQINDDYYEDLTAESVTKLLEALKATADSLPEQAVTWDAPAPTPTPGPKGEGIAGTDAESAIGKLLDKVVNGVRIPAPGPLSGRVTCEPASKLTSLTEPKWSTEVFREDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5GC02</accession>
    <name>D5GC02_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00005736001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PheA/TfdB FAD monooxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430098">
        <property type="protein sequence ID" value="CAZ82045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837854.1">
        <property type="nucleotide sequence ID" value="XM_002837808.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GC02"/>
    <dbReference type="STRING" id="656061.D5GC02"/>
    <dbReference type="GeneID" id="9181314"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005736001"/>
    <dbReference type="eggNOG" id="ENOG502QRH5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009665_9_2_1"/>
    <dbReference type="InParanoid" id="D5GC02"/>
    <dbReference type="OMA" id="WEVAFWN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016709">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012941">
        <property type="entry name" value="Phe_hydrox_C_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038220">
        <property type="entry name" value="PHOX_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050641">
        <property type="entry name" value="RIFMO-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43004:SF15">
        <property type="entry name" value="MONOOXYGENASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G03030)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43004">
        <property type="entry name" value="TRK SYSTEM POTASSIUM UPTAKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07976">
        <property type="entry name" value="Phe_hydrox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding" evidence="6">
        <location>
            <begin position="43"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="Phenol hydroxylase-like C-terminal dimerisation" evidence="7">
        <location>
            <begin position="450"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="7"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ82045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="639" mass="71077" checksum="4C229E8E419B4D1B" modified="2010-06-15" version="1">MPKFTKSGPNGNARISPSHLNALPRLTQPPPVSPTSPGGQERYEVVIVGSGPAGILSSVLLARYGITSRLTVDDKHSQTELGQADGLQPRTLEILQSLDLAHEILTQGCQFHEVSFWNPGPENKGIVRTNRVPNISVPARFLHEVTIHQGRIERILNEDLERNGGRVERGWRVTGFEIDTPVEEEGFPVVVRMVKTGEEWKGEERVVRCKYLLGADGARSVVRQGMGLRLEGDHTDHVWGVMDTVVKTNFPDIRRKSAIHSAVGSVIVIPRERISPTQAITRIYCQMEGEVEKDPNLDNQIQSQTKRVLSPYSIEFGEVDWWATYQIGQRVSPRFSQPDSRGTLRVHIAGDACHTHSPKSGQGMNVSMMDAYNFSWKVAHVLNGLVPPDSDLLATYQHERLDIAQQLIGFDREFSKMFSGRIGGDHGLSHEQFQGVYVKMCGFTSGCGIEYREGMLVKGGREPLKERGNEDEYLAPGRRVVNAKVLRFCDGNPRDIQDEDLPSTARYRILTLLPTSFKHFPHKYAELLTILTSRIPLTFPPSIVEVPIFFPGERGSLEWTDFPPSVREKSEWLVFGDEFGEVYAAWGVDEEVGALAVVRPDGYVGAVAALSEVVVVENWLGKVLVKGQTLGGTMLSSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCD8</accession>
    <name>D5GCD8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00005836001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430109">
        <property type="protein sequence ID" value="CAZ82181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837990.1">
        <property type="nucleotide sequence ID" value="XM_002837944.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCD8"/>
    <dbReference type="GeneID" id="9187114"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005836001"/>
    <dbReference type="eggNOG" id="ENOG502S4AX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_678243_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCD8"/>
    <dbReference type="OMA" id="HKSPYSH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050675">
        <property type="entry name" value="OAF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069">
        <property type="entry name" value="OLEATE-ACTIVATED TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069:SF28">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="6">
        <location>
            <begin position="43"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="104"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="170"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="104"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="172"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="195"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="219"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ82181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="406" mass="43362" checksum="605579FBA8E14D91" modified="2010-06-15" version="1">MSTAMLSQPATVAVPSAIATPPGPASPAKKRRRRAPATGAAEDCFTCRANGIKCDRRRPYCGPCLDVGMGCKGYRTQLTWGVGVASRGKLRGLHLPIQTPVADPKDAKKIVKKKGDEKTSGVSENGLRSKRSKTSLDGNGRDYDNSQRNGNSGVGKLSIITSYDFVNMEPPSNSSSAVSRTSSTRSANSSAISTGTSPALSGISRNSMPSQRITQGQRSPQSSSSYTPSYPASSQSQYRIQSQQQQYRQSPVHHYHKSPYSHVSLPPQQPTSSMLLSPMSEYGPKYESKYEPSFPSSQHYPMSSAPSTAPLYEMVTGVSSASYHPSASMTVTTTGVSYAPMVASMHATSRMMGDDHLHNPNHHHQNGGPGWNGVGVGIGNLHHQHVPAGTGNLSDLLYDDGMLGTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCE7</accession>
    <name>D5GCE7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00005855001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430109">
        <property type="protein sequence ID" value="CAZ82190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837999.1">
        <property type="nucleotide sequence ID" value="XM_002837953.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCE7"/>
    <dbReference type="FunCoup" id="D5GCE7">
        <property type="interactions" value="504"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GCE7"/>
    <dbReference type="GeneID" id="9186372"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005855001"/>
    <dbReference type="eggNOG" id="KOG3109">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059493_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCE7"/>
    <dbReference type="OMA" id="NACWVCE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008252">
        <property type="term" value="F:nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009166">
        <property type="term" value="P:nucleotide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006206">
        <property type="term" value="P:pyrimidine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052791">
        <property type="entry name" value="SSM1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47438">
        <property type="entry name" value="PHOSPHATE METABOLISM PROTEIN 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47438:SF1">
        <property type="entry name" value="PHOSPHATE METABOLISM PROTEIN 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ82190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="223" mass="25877" checksum="FC5F020FFE5141DA" modified="2010-06-15" version="1">MMKDLINKYFREQLRETFSVLLVHEAKLTVSLELNQEDAKTLHETYYKSYGLAIEGLVLHHKIDPLEYNGKVDDALPLDQVLHSDPQLRKLISDIDRTKVRLWLFTNAYITHGKRVVKLLGIEDLFDGITYCDYAQEILICKPKIEMFEKGLLYSRESCDPEILLTDYDSTINCQAAVRFGWENTVQKLEPSDPEPSVSVAKHNIRSLEELRALYPEFFKPPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCR3</accession>
    <name>D5GCR3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00005985001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430120">
        <property type="protein sequence ID" value="CAZ82306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838115.1">
        <property type="nucleotide sequence ID" value="XM_002838069.1"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GCR3"/>
    <dbReference type="GeneID" id="9187925"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005985001"/>
    <dbReference type="eggNOG" id="ENOG502SD64">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_257185_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCR3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1060"/>
            <end position="1082"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1094"/>
            <end position="1115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1189"/>
            <end position="1214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1234"/>
            <end position="1255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="194"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1278"/>
            <end position="1318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1338"/>
            <end position="1358"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1010"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="246"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1292"/>
            <end position="1304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ82306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1358" mass="156194" checksum="F5D0B3CF33F08BAD" modified="2010-06-15" version="1">MTKFVIDILSDDPVRHHETFILKPGATVKFTSVFKLDHENVPDIPPQYFDISPHLKPFGDRWWGFKTESSSFIFRRHLETILSHAMPLPWVDGGTHQPYVLHNHDLINMHPTWRSFINQAFYLLEIDKVLQQQNTADDETKSDYQKRIRNVIFGCFTFLLGDAERNGAENVSPYQPADLALPVKPDEAREVVGGEGIEEERDGLAGTAESSSKDKVVDTARPVGAPAGEVDSTDPQRDSSFLLETPGSTSSLNETSALHPGKTKDFIDSPPMRPIEPELFKYEASFEDCYRLLILLDKCLTTYPDETGLITRMVECFLKLVWQPIEMSKHQGSQLWPDRLERLGGSSPPYARFRMAPDNRNCGYGGQGIYVYDIMIQVAIWRAVRATNRLLDLFSGERDWSEWMVHQSLDDKAIRNRTIEAFLQPGTDTGQRSFPDRFISKIRGHVREPFREKRACTIAIPSLVDYFFFDDNNKPMSAWTETLKYCNTLSDSSKAQTPDTWGSFMCYQLATDTDRQELGERLEARAYSVGIFASDIVTPSSHGLCAPYWDIVTHFLSSHYAHPRIPKTDTPTGTNGAHGPMLPQEGLTNTGTNPGTSIMVGVRLDEKKSGRKGESLYMTTEDLRQVNDPPSYGERYWFRDPLFMKYEPKFEINRNSIVNFLEPGYSFVRGDIEGNWCCFWKTDEGRNRFLESIADMSNYNWGDNKNILIQPYRMIELDVSRGGDALLRMQKQRDLKRDKKRIIVMEDCAIEHLILLMANLDYQEAGHINQVLLRLTLMDSHEMRFEEQTIMPDNLWITEFNINFITTPSRQGVESSPLPNYFQRRQSEFLTMNDAAPNCIMVEASMGFRFIGDLHDQFWTCYVFYNFGRQELTTDTRARVEHIDSCKTLRQRKCLEGFLVCQALDLVLSQTKNVLNTITKSMGEGNKSQNIFIPLLMEDDLRGENYFKTMNKNSVFYPWLLDVYGALQDKCRETSTVAMRWISAEERRKYKPRWSEKDQMKFGEEIVQNRADVTARCAKLEKMSKNLEERINRIKNLKESLSSELALREARTSTQLARTINLFAVVTAIYLPLTFSTSIVAIQELHWKYPAKALVRIMLAVASGTLILLMNLSSLRRNLAALKRWAQRSIRNRMKGVPNRKKTSKKPICRDKRPAWNYWKKRARSLDEAEKRSALLTDNDIHGNESDWWYWYFTAIYITIVVPVQELTFIIRTLRGQKIENAGPLKKLVRLPGFPIWALQLALVYVIILAGYAFLSLVRFVHHTAVWLWTGNDTVESKKAAPEEVEQSSMSEPEDDAEGLESEPDSTQKDSTQKDTGLVDWLMKPAKIMMLLMVKEALQPKEEEREPDLENQGTPMEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCR9</accession>
    <name>D5GCR9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">DNA ligase</fullName>
            <ecNumber evidence="14">6.5.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">GSTUM_00005992001</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate.</text>
            <dbReference type="EC" id="6.5.1.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the ATP-dependent DNA ligase family.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.1" evidence="14"/>
    <dbReference type="EMBL" id="FN430120">
        <property type="protein sequence ID" value="CAZ82312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002838075.1"/>
    </dbReference>
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        <property type="interactions" value="656"/>
    </dbReference>
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    <dbReference type="GeneID" id="9187931"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005992001"/>
    <dbReference type="eggNOG" id="KOG0967">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005138_0_1_1"/>
    <dbReference type="InParanoid" id="D5GCR9"/>
    <dbReference type="OMA" id="CYATHSE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003910">
        <property type="term" value="F:DNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903461">
        <property type="term" value="P:Okazaki fragment processing involved in mitotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
    <dbReference type="CDD" id="cd07900">
        <property type="entry name" value="Adenylation_DNA_ligase_I_Euk"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.30:FF:000016">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3260.10">
        <property type="entry name" value="DNA ligase, ATP-dependent, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.30">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050191">
        <property type="entry name" value="ATP-dep_DNA_ligase"/>
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    <dbReference type="InterPro" id="IPR000977">
        <property type="entry name" value="DNA_ligase_ATP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012309">
        <property type="entry name" value="DNA_ligase_ATP-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012310">
        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016059">
        <property type="entry name" value="DNA_ligase_ATP-dep_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012308">
        <property type="entry name" value="DNA_ligase_ATP-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036599">
        <property type="entry name" value="DNA_ligase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00574">
        <property type="entry name" value="dnl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674:SF4">
        <property type="entry name" value="DNA LIGASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674">
        <property type="entry name" value="DNA LIGASE 1/3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04679">
        <property type="entry name" value="DNA_ligase_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01068">
        <property type="entry name" value="DNA_ligase_A_M"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="DNA_ligase_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ATP-dependent DNA ligase DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00697">
        <property type="entry name" value="DNA_LIGASE_A1"/>
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    </dbReference>
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        <property type="entry name" value="DNA_LIGASE_A2"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50160">
        <property type="entry name" value="DNA_LIGASE_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="7 14" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9 14" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="10 14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3 14" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-dependent DNA ligase family profile" evidence="17">
        <location>
            <begin position="702"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="22"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="30"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="58"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="108"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="208"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="221"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="252"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007572"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023172"/>
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            <dbReference type="ARBA" id="ARBA00023204"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00034003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAZ82312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="976" mass="107077" checksum="77612ADDCA79B985" modified="2010-06-15" version="1">MSSSKKQTTLGYVKSQTTLGRFFSLPAGTNPATPIQSKLKTISKPGKAADTDDVASPKVRKTSARGQARKKPTIPEDDGSDGGAGYTKAGVSFSETEMSDASEPAIGSSKQKALGNSSTKRERPAARKCGIKPSNIKKETETSEDEAPPSKRSKSAPTTKPLKKDCDESELSDEDIAPRKRLPIINSPKATAKKRKGKVVGKAVDVNTPVSPSGTTKGARSSPISSPVVSPQESGESEPPLPESPSSTPKISKSKSGSKAKVAKKSPSDKRSKKRGAASDTEAVDKPESSATHKALDSDSGTEKPSLAPAAGREMIKKLSKSVPPAYPDWVAGEPTPYAALVTTFNLLESTTKRLEKISHTSRFLRQVLQLSPDELLLVIHLMINKLAADFEGVEMGIGESLLMKAIGESCGRSLERIREDQRECGDLGLVAMKSRNNQKTLFAPRPLTIAVVHKGLLGIAKTKGEGGQGRKVSAIRKLLAAAKGDEAKFLIRGLEGKLRLGLAERTVLVSLSSAMITHEQELVGKTPTTAMLDQSELNLRSVYSELPSYEIIIPAMREHGIMDIKKYCKLQPGVPVKPMLAKPTKAISEVLDRFENKRFTCEYKYDGERAQIHYVSPKSSIEYPPATLSGEPIKNLAKVFSRNSEDLSGKYPDILAAMNEWVRPEVESFVLDCEAVGWDETNNRLLPFQMLQTRKKKDVALEDVKIRVCVHAFDLLFLNGKPVVRESLDDRRKLMHKTFIEVPNQFVFARYMDSSHLEEIRLFLDQSVKDSCEGLMVKMMDGPESFYEPSQRSRNWLKIKKDYIDELGDSLDLVVIGAYWGRGKRTSVYGSFLLACYNPGTQNYESLCNIGTGFTEAELEEFYGTLHPLEIDRAKPFYDHPSGNAGKPDVWFEPKVLWEVKAADLTLSPRYKAAAGLAGHPDKGVSLRFPRFIGPRTDKTGEDATSSSQIAEMYLRQDQVAHKTGAKGVDDDFEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCY5</accession>
    <name>D5GCY5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00000869001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430129">
        <property type="protein sequence ID" value="CAZ82378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838187.1">
        <property type="nucleotide sequence ID" value="XM_002838141.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCY5"/>
    <dbReference type="STRING" id="656061.D5GCY5"/>
    <dbReference type="GeneID" id="9183840"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000869001"/>
    <dbReference type="eggNOG" id="ENOG502S03K">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018003_1_0_1"/>
    <dbReference type="InParanoid" id="D5GCY5"/>
    <dbReference type="OMA" id="PMLFGDP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G" evidence="2">
        <location>
            <begin position="38"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="264"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ82378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="375" mass="43179" checksum="1B0326C3F2BCAF4D" modified="2010-06-15" version="1">MANSPSSTMESSVANTPPSAWDVEEVDYKTLMEDEIVIAVMGVTGAGKSHLIRQVTGRNEIAVGDGLESCTDKIASYRFKHDGLNITLVDTPGFNDTYRTDTDVLLDIATWMEFSYRENFKLSGIIYLHPISAVKMEGSALRNLRMFRKLCGEDALNHVFLATTHWKNVPEELGRRRVKELRENPEFWKGMLSRGAKVAKYLGDRESGLDLIDVLVKMGRVTLGIQHEIVNEGKELYETAAGEAVNAELARLQRNHETALNAVKAEWMQALRDKDEELKEVLSEERQRIEEKLAQVENDRENLRRERRREIREQEDEWHRRVKNMEGAQQKEREEFQQRFRELQASVRDGESRIPWMSLIDLVAKGFVHFMNSRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GD61</accession>
    <name>D5GD61_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00006054001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430142">
        <property type="protein sequence ID" value="CAZ82454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838263.1">
        <property type="nucleotide sequence ID" value="XM_002838217.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GD61"/>
    <dbReference type="STRING" id="656061.D5GD61"/>
    <dbReference type="GeneID" id="9183918"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006054001"/>
    <dbReference type="HOGENOM" id="CLU_2484958_0_0_1"/>
    <dbReference type="InParanoid" id="D5GD61"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="87" mass="9976" checksum="DA13EFD1246B66F2" modified="2010-06-15" version="1">MSSSRFPRKFTPPASPTASYYDISDDDENGYSTITNTSTSRSVKLLRYVETRNYWSILSYAIHAFKMFLKLTFFWSVGLCAPNALEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDB9</accession>
    <name>D5GDB9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Histone H3-like centromeric protein CSE4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">CENP-A homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">CENPA homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">GSTUM_00006141001</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Histone H3-like nucleosomal protein that is specifically found in centromeric nucleosomes. Replaces conventional H3 in the nucleosome core of centromeric chromatin that serves as an assembly site for the inner kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of centromeric nucleosomes, where DNA is wrapped around a histone octamer core. The octamer contains two molecules each of H2A, H2B, CSE4/CENPA and H4 assembled in one CSE4-H4 heterotetramer and two H2A-H2B heterodimers. Interacts with the inner kinetochore.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the histone H3 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430142">
        <property type="protein sequence ID" value="CAZ82512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838321.1">
        <property type="nucleotide sequence ID" value="XM_002838275.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDB9"/>
    <dbReference type="STRING" id="656061.D5GDB9"/>
    <dbReference type="GeneID" id="9183976"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006141001"/>
    <dbReference type="eggNOG" id="KOG1745">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078295_3_3_1"/>
    <dbReference type="InParanoid" id="D5GDB9"/>
    <dbReference type="OMA" id="VHLFEDC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22911">
        <property type="entry name" value="HFD_H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000087">
        <property type="entry name" value="Probable histone 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000164">
        <property type="entry name" value="Histone_H3/CENP-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11426">
        <property type="entry name" value="HISTONE H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00622">
        <property type="entry name" value="HISTONEH3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00428">
        <property type="entry name" value="H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00959">
        <property type="entry name" value="HISTONE_H3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0137">Centromere</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="15">
        <location>
            <begin position="44"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="17"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00043846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAZ82512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="152" mass="16861" checksum="C0C6CF1149E67A0B" modified="2010-06-15" version="1">MAKAKAIARKSTGGKAPLKKPTKTVKRPSDPVPAKPTTARRWRPGTRALREIRQYQKSTDLLIRKLPFARLVREIAMDYTNAAHLNGLGGSGVDDLRWQSTAILALQEAAEAYLVHLFEDSNLLAIHAKRVTIMQKDIQLARRIRGAWGGLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDF6</accession>
    <name>D5GDF6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00006191001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430142">
        <property type="protein sequence ID" value="CAZ82549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838358.1">
        <property type="nucleotide sequence ID" value="XM_002838312.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDF6"/>
    <dbReference type="GeneID" id="9184012"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006191001"/>
    <dbReference type="HOGENOM" id="CLU_3351400_0_0_1"/>
    <dbReference type="InParanoid" id="D5GDF6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ82549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="37" mass="4305" checksum="CE99CC4DBC6D2A83" modified="2010-06-15" version="1">MAEEGGWISVRLFQFRDSKFVLGLLEPQMRVPRPTGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDM7</accession>
    <name>D5GDM7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="14">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00001123001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430152">
        <property type="protein sequence ID" value="CAZ82620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838429.1">
        <property type="nucleotide sequence ID" value="XM_002838383.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDM7"/>
    <dbReference type="FunCoup" id="D5GDM7">
        <property type="interactions" value="274"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GDM7"/>
    <dbReference type="GeneID" id="9181624"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001123001"/>
    <dbReference type="eggNOG" id="KOG1001">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000315_2_0_1"/>
    <dbReference type="InParanoid" id="D5GDM7"/>
    <dbReference type="OMA" id="CHPHLIN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18008">
        <property type="entry name" value="DEXDc_SHPRH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16449">
        <property type="entry name" value="RING-HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050628">
        <property type="entry name" value="SNF2_RAD54_helicase_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626:SF16">
        <property type="entry name" value="ATP-DEPENDENT HELICASE ULS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="362"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="706"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="889"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="65"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="237"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="797"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="22"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="75"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="261"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="797"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="822"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="862"/>
            <end position="876"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ82620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1062" mass="117491" checksum="E177702C38BABD43" modified="2010-06-15" version="1">MKRNREDAFDHFHQDFPETKSQRTTPSPSLQSALLNPNFAGVRHSPLSRDTGISDEELARSLHAELNSPRVGNGMSFNNPGGSSDQSLPSINSGLAGMCQSNSDASYSGRNLHPRRHEEYMPRASLRHVGSSNGASSSYLSEYDDGYNYSDSGYLDNTLSPALLGGLPKPSATPLRFPHVSANEVIELLDSDEESEAAVSQSSIGSLLPAQDSRAGSVRPFDYSNYDNLATTNGWNNNNGGGFDDPIIVPNQMPQTPDPATASNDNGNQTPQASDSSAYGGIVDRDGNPLNSGWPSTYRPEIGNYINHIRNDPTKTLDQLKTLMENIRPDMEIPPENREGTPDDMTYPLMEHQKLGLAWLKGMEDGSNKGGILADDMGLGKTIQALALIVSRKSKDPDRKTTLIVAPIALLKQWEREIERKLKAEHRLKVIIHHGNQKKCRSFEGFKDYDVVLTTFGTIGTEYKKKQALLESEDPEATKNANFFFVGDHCEWYRVIIDEAQCIKNKDTQSAKGCCALNAKFRLCLSGTPMQNSCDEMFSLLRFLRIEPYSSWSEFSNTFSRPLKSKSERAVSSALLKLQALMKAVLLRRTKDSTIDGKPILTLPDKSIEMVYAVLSPDEQQFYQALQDKSKILYNKYLRAGTVGRNYSNILVLLLRLRQACCHPHLIRDIEVADAKKPFDDQMIELAKSLSPEAVARLKDPDAFECPICLDAADNPSIVIPCGHQFCSECLVQLHTQHVDAAIASGNEEGGARCPGCRGAFQLNKVIDLRTFQKVHMSEGEEAAPEEPEEEYDDDLIYDTDSSEDSDDDGDTGSDLEGFIVNDEEVDSDEQGEGSATVGASEDGGQAPARKAAAPKKKKDVKGKGKAKEKSRPRTLAEVRKDAMRNAKSKKQYMKHLQKRWISSAKKTIVFSQFTSLLDLIEIPIHQKGWTYRRYDGGMTSTARNEALTEFTDDPSVNIILVSLKAGNSGLNLVAASQVIILDPFYNPFIENQAIDRAHRIGQQRKVRVHKLIVAGTVEDRVLALQEEKRKLIEGALDEKASQGIGRLSSKDLGFLFGIESR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDW1</accession>
    <name>D5GDW1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Polynucleotide 5'-hydroxyl-kinase GRC3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Polynucleotide 5'-hydroxyl-kinase grc3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00006304001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Polynucleotide 5'-kinase involved in rRNA processing.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Clp1 family. NOL9/GRC3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430153">
        <property type="protein sequence ID" value="CAZ82704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838513.1">
        <property type="nucleotide sequence ID" value="XM_002838467.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDW1"/>
    <dbReference type="FunCoup" id="D5GDW1">
        <property type="interactions" value="141"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GDW1"/>
    <dbReference type="GeneID" id="9184499"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006304001"/>
    <dbReference type="eggNOG" id="KOG2750">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010345_1_1_1"/>
    <dbReference type="InParanoid" id="D5GDW1"/>
    <dbReference type="OMA" id="PEFAPMG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051731">
        <property type="term" value="F:polynucleotide 5'-hydroxyl-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000448">
        <property type="term" value="P:cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045116">
        <property type="entry name" value="Clp1/Grc3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032319">
        <property type="entry name" value="CLP1_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12755">
        <property type="entry name" value="CLEAVAGE/POLYADENYLATION FACTOR IA SUBUNIT CLP1P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12755:SF3">
        <property type="entry name" value="POLYNUCLEOTIDE 5'-HYDROXYL-KINASE NOL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16575">
        <property type="entry name" value="CLP1_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Clp1 P-loop" evidence="10">
        <location>
            <begin position="291"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="27"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ82704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="669" mass="72387" checksum="9657D91FBD2D42CD" modified="2010-06-15" version="1">MPKRQREDEPKQAPLSALAARRLRWLTAGAQHPESKEVERVGKEKEEGKALELSNEETHRLREVEMELKKGGGSGGGKRVVISHVQISSGGGEAVPTYSAEAMAAAPGGQGGVETETDTKVDKEVRMSNFTLTSENYIPAQDGSCVVRMAPGETLVILGEYELRVRKGAITISGAKLTRQSGCCQVFAPSTHALPEIACVKVKRKGEDSDAEVLFTQAESGIRNLGRTCPVANGIWVPVGGDGGRSFHPLFTTSAPLPVLTIPKQWEETLGGIEKVANPGNTAPPVILLSGGKSVGKSTLSRIFTNRLLTAETYPRVYYLDVDPGQPSFTPPGFLSLHLLERPILGPPFTSPLLPKTLITAHHIGYTSPREDPSAYIRAILALLSDYRSAAPTDPGPLLINSAGWIKSLGLELLREIIQASCTTDIVYITSTLDHLIPIMDVLPPTNPPRMHVLISSATSENARFTAADLRTLQTSSFIHYCPDTLRWVVKPLAEIQPWRLPYAGTHPAIDGVSILHEEDLPPTELLTAIEGTLVAVVEVDEDEESITETAKIVLTPEELPYLPTPPAPERSRAVGMALIKGVDVEKREVWVVATLSSDDAERLRGKRTVLVRGRLELCAAEMVAGVGEDYTRPGVPWVSFGGGRTGDGKGSSAWRVRRNLMRKGQLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDZ8</accession>
    <name>D5GDZ8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="11">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00001153001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430157">
        <property type="protein sequence ID" value="CAZ82741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838550.1">
        <property type="nucleotide sequence ID" value="XM_002838504.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDZ8"/>
    <dbReference type="GeneID" id="9183073"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001153001"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_43_5_1"/>
    <dbReference type="InParanoid" id="D5GDZ8"/>
    <dbReference type="OMA" id="PRWYLTK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015798">
        <property type="term" value="P:myo-inositol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015791">
        <property type="term" value="P:polyol transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050814">
        <property type="entry name" value="Myo-inositol_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020:SF25">
        <property type="entry name" value="SUGAR TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_7G05830)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="210"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="249"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="374"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="401"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="428"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="467"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="500"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="75"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="22"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ82741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="595" mass="67359" checksum="E1A79D3E7DD96BA0" modified="2010-06-15" version="1">MSNPISSTLGSPDAEKPLSVGPHEHQPASKNQPEDLQKLNSKLAHPLSGIPHEELMEMGASYARENGMEDLQDEFRKGALLAQKPEAFDTLPLLTDDDKYYLRREVTHKWSHPKDLYHMVVMCSMAAAVQGMDETVINGANLFFLKQFGIENREWIAGLVNSAPYLSCFTVGCWLTHPLNKQFGRFGIGPKSSTVPVYAAECSPPAIRGALVMMWQMWTAFGIMLGYIADVAFYNVKDPPHITGLNWRLMLASAMVIPLIVCFQVYFCPESPRYEMLKGRHKRAFDSLARLRWTKVQAARDLFYMHTLLRVEAELNKKSGYKLVEFFTIGRNRRAMIGSEIVMFMQQFCGVNVIAYYSSVIFKESGFTVIDSLLASLGFGIINFLFAIPAVYTIDTFGRRFLLLVTFPLMGLFLLFTGSSFWIPDREARIGCVALGIYLFGIVYSPGEGPVPFTYSAEAYPLYIRDLGMSLATATTWGFNWLLSMTWLPLVRAFKPQGAFGYYAAWNGIGFVLVLLFLPETKGRTLEELDRVFEVPTRSHMKYGLAQVPWFFRRYIFRQDVPAPKILELEELEDIEYHPDAFRNGSKDIGEGNSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>D5GE53</accession>
    <name>D5GE53_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00006383001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430164">
        <property type="protein sequence ID" value="CAZ82796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838605.1">
        <property type="nucleotide sequence ID" value="XM_002838559.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GE53"/>
    <dbReference type="STRING" id="656061.D5GE53"/>
    <dbReference type="GeneID" id="9183128"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006383001"/>
    <dbReference type="eggNOG" id="KOG3337">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067902_0_0_1"/>
    <dbReference type="InParanoid" id="D5GE53"/>
    <dbReference type="OMA" id="MKFFENN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006797">
        <property type="entry name" value="PRELI/MSF1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037365">
        <property type="entry name" value="Slowmo/Ups"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11158">
        <property type="entry name" value="MSF1/PX19 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04707">
        <property type="entry name" value="PRELI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50904">
        <property type="entry name" value="PRELI_MSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRELI/MSF1" evidence="1">
        <location>
            <begin position="2"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="240" mass="27125" checksum="239D8A51BD933FD1" modified="2010-06-15" version="1">MVKFYENSFSYDYQWPAVTLAYFLRYPNPYSKHVVSSDTLSSQLDPQTGNLHVTRLHLKRGKLPASLARFLPKIKESYILEKSIVDVRSQRLETETRNLDWEGVLSVVESQVYTPGNDSTASGLEAPVAGAGKTDVTTVVRFESRLGGAARNTQSDGMTGEGVKDGWFLGWTTSSVQGSIELVGVRRMREGFGRRREGMKVVLEQLRERGFVGVINEQRQGGWERWKKVWRGEGVERMDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEK8</accession>
    <name>D5GEK8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00006541001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430175">
        <property type="protein sequence ID" value="CAZ82951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838760.1">
        <property type="nucleotide sequence ID" value="XM_002838714.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEK8"/>
    <dbReference type="STRING" id="656061.D5GEK8"/>
    <dbReference type="GeneID" id="9181581"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006541001"/>
    <dbReference type="HOGENOM" id="CLU_1679242_0_0_1"/>
    <dbReference type="InParanoid" id="D5GEK8"/>
    <dbReference type="OMA" id="DGMMEMG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="96"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="157" mass="16209" checksum="224D287169F1B809" modified="2010-06-15" version="1">MGSMVGMMSGMGGMPGMTPGMGGTMGMSGMDGMMEMGGMPTMMGVLGMGGMSGMGDMASAMGGMMGGIRPSVMPYTMAHMGQPFMMGGAGSRMSNMMGNMGNRMSGAMGGMRNKMGEGTSRMGRKSRMSSSSRTEKPASPSTDTDEDVDDEEDREEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5GF21</accession>
    <name>D5GF21_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">cyclin-dependent kinase</fullName>
            <ecNumber evidence="2">2.7.11.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00006707001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="2"/>
    <dbReference type="EMBL" id="FN430197">
        <property type="protein sequence ID" value="CAZ83114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838923.1">
        <property type="nucleotide sequence ID" value="XM_002838877.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GF21"/>
    <dbReference type="STRING" id="656061.D5GF21"/>
    <dbReference type="GeneID" id="9187868"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006707001"/>
    <dbReference type="eggNOG" id="KOG0594">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_181_6_1"/>
    <dbReference type="InParanoid" id="D5GF21"/>
    <dbReference type="OMA" id="FELWTVF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030332">
        <property type="term" value="F:cyclin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010389">
        <property type="term" value="P:regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF576">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE CSK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="118"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="63"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ83114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="414" mass="46085" checksum="5B665274564BD8A8" modified="2010-06-15" version="1">MTPLPSQEWKTTFSFRERVAIVEHIASLLESSDPSLEHSRALSSARAIEVESHEKSFSQEEYTRLWKAKLQELKEKYAEKQLETRSLSAVSGSGSAFDDAASQDIPHATDRGITIGRFIGCRHHNSGIFSDVYKALLKPMTLMSPAQFVALKVTIPSRMAPPHDSHREARILQKSEHRCVVKLLEKFTLPTSQFVMVFPFYNYSLDTLLKSNAKIAGDQMADIFQDVLCGLEHIHSLGIIHRDVKPSNILLMGADGPAVLADFGIAWSSNDPESEPAEHKITDVGTTCYRPPEILFGNTAYTTSLDMWAFGCVMAECYTDGRESLFDAGDLGSELQLIGSIFRRLGTPTLETWPEASNFSDFGKICFNSFPAQKWEDILPKAPRPVTELLSQLLVFESGDRLSATEALATDLFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>D5GF63</accession>
    <name>D5GF63_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00006742001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430208">
        <property type="protein sequence ID" value="CAZ83156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GF63"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006742001"/>
    <dbReference type="HOGENOM" id="CLU_1897707_0_0_1"/>
    <dbReference type="InParanoid" id="D5GF63"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="134" mass="15381" checksum="FD4A4A7254562E21" modified="2010-06-15" version="1">MAYTQLLRFPAPRASKRKYSLPTQVHPLPKRVKQTYPIYSSSSNAKDFPISNPSGNTPAPHLTSLRTPAEQSRMRQITSRIHHLQKQSAPESHVGALHPYIWRPSEEEVTLESYRYPIRSNGAFQSWEILRATE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFD5</accession>
    <name>D5GFD5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00006841001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the avfA family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430208">
        <property type="protein sequence ID" value="CAZ83228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839037.1">
        <property type="nucleotide sequence ID" value="XM_002838991.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFD5"/>
    <dbReference type="FunCoup" id="D5GFD5">
        <property type="interactions" value="642"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GFD5"/>
    <dbReference type="GeneID" id="9188084"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006841001"/>
    <dbReference type="eggNOG" id="KOG1203">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025711_1_0_1"/>
    <dbReference type="InParanoid" id="D5GFD5"/>
    <dbReference type="OMA" id="DYVAWSA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15020">
        <property type="entry name" value="FLAVIN REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15020:SF50">
        <property type="entry name" value="UPF0659 PROTEIN YMR090W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13460">
        <property type="entry name" value="NAD_binding_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="2">
        <location>
            <begin position="8"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ83228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="244" mass="26290" checksum="5C5E2FA8EAC27989" modified="2010-06-15" version="1">MVNVLVLGGHGKIAQIISKILAQKGHQITSVIRDPSQSSMVESLQRNVAPLVSSLEESTPSSIRKEMENINWVVWSAGAGGKGGPQRTKAVDEIAAKKYIDAAVETPSVDTFLMVSASVARRVSASWWDESGVETYKGGWDTIGAYCEAKTAADEYLYEVGRKWGIRDICLRPGRLSDDPATGLVSLGRCKATGEVARGDVADVAVRLLESDVVGGLWIDMIGGDKKVEEEVSRVVRDKVTAKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFF0</accession>
    <name>D5GFF0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">alcohol dehydrogenase</fullName>
            <ecNumber evidence="3">1.1.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00006862001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.1" evidence="3"/>
    <dbReference type="EMBL" id="FN430208">
        <property type="protein sequence ID" value="CAZ83243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839052.1">
        <property type="nucleotide sequence ID" value="XM_002839006.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFF0"/>
    <dbReference type="FunCoup" id="D5GFF0">
        <property type="interactions" value="798"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GFF0"/>
    <dbReference type="GeneID" id="9188191"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006862001"/>
    <dbReference type="eggNOG" id="KOG0023">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_20_1_1"/>
    <dbReference type="InParanoid" id="D5GFF0"/>
    <dbReference type="OMA" id="YKGLKMT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08297">
        <property type="entry name" value="CAD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000039">
        <property type="entry name" value="Alcohol dehydrogenase AdhP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.180.10:FF:000002">
        <property type="entry name" value="Alcohol dehydrogenase AdhP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940:SF3">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="9">
        <location>
            <begin position="14"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ83243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="349" mass="37137" checksum="A75CEB9EF43AD4CF" modified="2010-06-15" version="1">MSVPTEQYAQVFKSNNDPIERKKVPVSKPGPDEVLVNIKYTGVCHTDLHAWKGDWPLQTKQNLIGGHEGAGVVVAVGELVKDIKVGDHAGIKWINGSCGHCEFCMAGDEPLCTNALLSGYTVDGSFQQYAIGKAVDVARIPKGVPLDAVAPILCAGITVYKALKESQARAGQTIAIVGAGGGLGSLAVQYAKAMGFRVIAIDTGSEKQEMCLNVLGAEEYVDFAKEDVGAKVKSITGGLGAHAVILLAVSEKPFQHATEYCRAHGTVVCVGLPAKARISAEVFPTVIRMITIKGSYVGNRIDTHEAIDFFARGLIKAPFKVGKLSELPQVFKLMEEGKIAGRYVLDTSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFP9</accession>
    <name>D5GFP9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">tRNA (guanine(26)-N(2))-dimethyltransferase</fullName>
            <ecNumber evidence="7">2.1.1.216</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">tRNA 2,2-dimethylguanosine-26 methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">tRNA(guanine-26,N(2)-N(2)) methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">tRNA(m(2,2)G26)dimethyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00001907001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 12">
            <text>guanosine(26) in tRNA + 2 S-adenosyl-L-methionine = N(2)-dimethylguanosine(26) in tRNA + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:43140"/>
            <dbReference type="Rhea" id="RHEA-COMP:10359"/>
            <dbReference type="Rhea" id="RHEA-COMP:10360"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74513"/>
            <dbReference type="EC" id="2.1.1.216"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the class I-like SAM-binding methyltransferase superfamily. Trm1 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.216" evidence="7"/>
    <dbReference type="EMBL" id="FN430219">
        <property type="protein sequence ID" value="CAZ83342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839151.1">
        <property type="nucleotide sequence ID" value="XM_002839105.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFP9"/>
    <dbReference type="FunCoup" id="D5GFP9">
        <property type="interactions" value="1139"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GFP9"/>
    <dbReference type="GeneID" id="9188292"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001907001"/>
    <dbReference type="eggNOG" id="KOG1253">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010862_2_0_1"/>
    <dbReference type="InParanoid" id="D5GFP9"/>
    <dbReference type="OMA" id="PNPTPYW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160104">
        <property type="term" value="F:tRNA (guanine(26)-N2)-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002940">
        <property type="term" value="P:tRNA N2-guanine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.56.70:FF:000001">
        <property type="entry name" value="tRNA (guanine(26)-N(2))-dimethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000051">
        <property type="entry name" value="tRNA (guanine(26)-N(2))-dimethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.70">
        <property type="entry name" value="N2,N2-dimethylguanosine tRNA methyltransferase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002905">
        <property type="entry name" value="Trm1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042296">
        <property type="entry name" value="tRNA_met_Trm1_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00308">
        <property type="entry name" value="TRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10631">
        <property type="entry name" value="N 2 ,N 2 -DIMETHYLGUANOSINE TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10631:SF3">
        <property type="entry name" value="TRNA (GUANINE(26)-N(2))-DIMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02005">
        <property type="entry name" value="TRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51626">
        <property type="entry name" value="SAM_MT_TRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 12" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 12" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 12" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="1 12" id="KW-0820">tRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="46"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="646"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="656"/>
            <end position="674"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00077143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00082896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00083299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ83342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="674" mass="73929" checksum="BD8D61EE610564C8" modified="2010-06-15" version="1">MTPSFLVGSTLRPHSHSLNLRVLSPTGFAKIHSRAFSKYSTLLNSDHHSQKLPSHPQTPAPAATTRMGKRKRTPNQGPIAPNPAPAHAFPVTLEDGTECTAVREGLATVLFPRFVAKKDKSGNDLVDGQVFYNPVQQFNRDLSVLAIRAFGEVFLREKKAKKAAKMKGDEDERPMKADIPPKFSILDALSATGLRALRYALEIPFVTHTTSNDLDPSASQSIRRNIHFNKTHTTKTVDPALTPDAALSKIQSRDYPSSKYDVIDLDPFGTAVPFLDAAVQAVSDGGLLCITCTDAGVWASMGYSEKCFSLYGGMPIKGEWSHEAGLRLILYATATTAARYGFSIEPLLSLSIDFYARVFVRVRKSPREVKKLASTSMIVYNCDSGCGSWRTQSLGKRKEEKNKNGDGTYLKFGLAMAPTTSSECPDCGFPMHLGGPMWSGPLHSPTFITHLLSALPAQPPEIYQTLPRITGMLQTALQESTLPNSPFFFVTNCLARTLHCEAPPLAAFRGALMRLGYTVVRSHCKPTSVKTDAPWSLIWEVMRRWVRQKPAGQGKVGGAMAGYKILKFGIKENADNGEGSAREKWRAEKEKEEMERLTALEIVFDEGLGKDKDRVKGVVRYQVNPTANWGPLKRGRGVEALIGETTAVEEGGGGELVERGEESDEEGRVSKEAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFX2</accession>
    <name>D5GFX2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00001921001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430222">
        <property type="protein sequence ID" value="CAZ83415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839224.1">
        <property type="nucleotide sequence ID" value="XM_002839178.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFX2"/>
    <dbReference type="GeneID" id="9181764"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001921001"/>
    <dbReference type="HOGENOM" id="CLU_2759643_0_0_1"/>
    <dbReference type="InParanoid" id="D5GFX2"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ83415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="70" mass="7649" checksum="5D74E43467FB1970" modified="2010-06-15" version="1">MPGHLRNLPCPTSYRGLFLFHPPIYLSPSSLHFPGTSFTTNTIIRTLLIAGGVLVTSSASKSSFPYRGIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFZ4</accession>
    <name>D5GFZ4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">mRNA-capping enzyme subunit beta</fullName>
            <ecNumber evidence="8">3.6.1.74</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">mRNA 5'-phosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">mRNA 5'-triphosphate monophosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00001944001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">First step of mRNA capping. Converts the 5'-triphosphate end of a nascent mRNA chain into a diphosphate end.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 5'-end triphospho-ribonucleoside in mRNA + H2O = a 5'-end diphospho-ribonucleoside in mRNA + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67004"/>
            <dbReference type="Rhea" id="RHEA-COMP:17164"/>
            <dbReference type="Rhea" id="RHEA-COMP:17165"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:167616"/>
            <dbReference type="ChEBI" id="CHEBI:167618"/>
            <dbReference type="EC" id="3.6.1.74"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:67005"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterodimer. The mRNA-capping enzyme is composed of two separate chains alpha and beta, respectively a mRNA guanylyltransferase and an mRNA 5'-triphosphate monophosphatase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the fungal TPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.74" evidence="8"/>
    <dbReference type="EMBL" id="FN430229">
        <property type="protein sequence ID" value="CAZ83437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839246.1">
        <property type="nucleotide sequence ID" value="XM_002839200.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFZ4"/>
    <dbReference type="STRING" id="656061.D5GFZ4"/>
    <dbReference type="GeneID" id="9181782"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001944001"/>
    <dbReference type="eggNOG" id="ENOG502RZAX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018004_2_1_1"/>
    <dbReference type="InParanoid" id="D5GFZ4"/>
    <dbReference type="OMA" id="HHMIMTR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031533">
        <property type="term" value="C:mRNA capping enzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140818">
        <property type="term" value="F:mRNA 5'-triphosphate monophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004651">
        <property type="term" value="F:polynucleotide 5'-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006370">
        <property type="term" value="P:7-methylguanosine mRNA capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07470">
        <property type="entry name" value="CYTH-like_mRNA_RTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.100.10">
        <property type="entry name" value="mRNA triphosphatase Cet1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040343">
        <property type="entry name" value="Cet1/Ctl1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033469">
        <property type="entry name" value="CYTH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004206">
        <property type="entry name" value="mRNA_triPase_Cet1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037009">
        <property type="entry name" value="mRNA_triPase_Cet1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28118:SF1">
        <property type="entry name" value="POLYNUCLEOTIDE 5'-TRIPHOSPHATASE CTL1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28118">
        <property type="entry name" value="POLYNUCLEOTIDE 5'-TRIPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02940">
        <property type="entry name" value="mRNA_triPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55154">
        <property type="entry name" value="CYTH-like phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0506">mRNA capping</keyword>
    <keyword evidence="4 8" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="mRNA triphosphatase Cet1-like" evidence="10">
        <location>
            <begin position="254"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="444"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="51"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="106"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="154"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="209"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ83437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="535" mass="58735" checksum="C16387E85917A84F" modified="2010-06-15" version="1">MMDLDGGSGVTPGPVLGPGQKWGSPVMERAKPNGTISVDSRDSQGSHHLAPQQQQPQPTQLPSTSNSNSNLSQQPSSQPSSGASSQRTSTVSPSNKEQPHPPPSTLTPQTKKSTPTPTNISPRHPPPQPSSSPKGAHQLGPPPTPSQNQAIVEGGTSSSPGSSGPPKKRARRDEAPIWARKASRSSSSSPVLANRRQPSSANQHQPPLQQQPPPLPPPPPQQHQKPPPASGFTPRFVPCFGAWEPSICNTEPYEELTREVANFLFNQVVCQNDPALTSSGGDGPVVMIEIEAKIGHLIDKERGERLKLPVVTETVLNVDEPGWKIQFKSSMTEVILLFIYLFSSTPTRGANSRPPAGPQRIPMDYVHTRERDTFFELPPDKFQALPNILRSYMRHKPKVRVTIDQKTGKVINKIIKTRIADLNVYSPKTAFDWRVSANIEMPYPGDIDGLQPSSDRGGGDRNKDRLSYRHLAYQIDLTQVTDNTARKEHELEVEVSGQEIIRHGNLAREKKPNAYEYLVRGFVDNVRVLIRAIEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>D5GG27</accession>
    <name>D5GG27_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00001964001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430233">
        <property type="protein sequence ID" value="CAZ83470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839279.1">
        <property type="nucleotide sequence ID" value="XM_002839233.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GG27"/>
    <dbReference type="STRING" id="656061.D5GG27"/>
    <dbReference type="GeneID" id="9181816"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001964001"/>
    <dbReference type="eggNOG" id="KOG2509">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2442476_0_0_1"/>
    <dbReference type="InParanoid" id="D5GG27"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004828">
        <property type="term" value="F:serine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006434">
        <property type="term" value="P:seryl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002317">
        <property type="entry name" value="Ser-tRNA-ligase_type_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11778">
        <property type="entry name" value="SERYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ83470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="90" mass="10182" checksum="5DB1B19E0C1E5983" modified="2010-06-15" version="1">MFDEAPRNPEIRYGTNSQSDTVKEYVHCLNSTLCTTERSLCYLLENYQTPEYINVPEPLRKYIPGAPGLLPFSKELPTNNTSMGRGNPKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGB8</accession>
    <name>D5GGB8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00002007001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430254">
        <property type="protein sequence ID" value="CAZ83561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839370.1">
        <property type="nucleotide sequence ID" value="XM_002839324.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGB8"/>
    <dbReference type="STRING" id="656061.D5GGB8"/>
    <dbReference type="GeneID" id="9183159"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002007001"/>
    <dbReference type="HOGENOM" id="CLU_2759645_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGB8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003954">
        <property type="term" value="F:NADH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015990">
        <property type="term" value="P:electron transport coupled proton transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003945">
        <property type="entry name" value="NU5C-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42829">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42829:SF2">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072079" evidence="5">
        <location>
            <begin position="26"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="NADH:quinone oxidoreductase/Mrp antiporter transmembrane" evidence="6">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ83561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="70" mass="7849" checksum="533D0CC06E894659" modified="2010-06-15" version="1">MAKIQIFLLHVWLPLAMEGPTPVSALIHAATMVCTTKDNNINILISLLYTQMGNQRNIKINKEMVQLTSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGS3</accession>
    <name>D5GGS3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007492001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430275">
        <property type="protein sequence ID" value="CAZ83695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839504.1">
        <property type="nucleotide sequence ID" value="XM_002839458.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGS3"/>
    <dbReference type="GeneID" id="9181716"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007492001"/>
    <dbReference type="HOGENOM" id="CLU_1662084_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGS3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="159" mass="18653" checksum="42C0EBAEBEEF2D75" modified="2010-06-15" version="1">MSRSCCIIRYHRNKRANVPCEPLHFSSPKRTIPSRQSFSLAVVRLSDVSLSLRNRYLRQFLNHCLLTTCLPLLSLFLITVKRFFFRSVRLSVKMILIPVLRFWSTLSSFILTNGTASLRIQKFWLMRPPNSMLHLSTLFELVFGPHFVSKRYIAQYQVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGV6</accession>
    <name>D5GGV6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00002052001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430276">
        <property type="protein sequence ID" value="CAZ83749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839558.1">
        <property type="nucleotide sequence ID" value="XM_002839512.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGV6"/>
    <dbReference type="FunCoup" id="D5GGV6">
        <property type="interactions" value="224"/>
    </dbReference>
    <dbReference type="GeneID" id="9181719"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002052001"/>
    <dbReference type="eggNOG" id="KOG1994">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046724_2_0_1"/>
    <dbReference type="InParanoid" id="D5GGV6"/>
    <dbReference type="OMA" id="DYMNMVI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025239">
        <property type="entry name" value="DUF4187"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039249">
        <property type="entry name" value="GPATCH11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21032">
        <property type="entry name" value="G PATCH DOMAIN-CONTAINING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21032:SF0">
        <property type="entry name" value="G PATCH DOMAIN-CONTAINING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13821">
        <property type="entry name" value="DUF4187"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01585">
        <property type="entry name" value="G-patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01173">
        <property type="entry name" value="DUF4187"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00443">
        <property type="entry name" value="G_patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G-patch" evidence="2">
        <location>
            <begin position="119"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="38"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ83749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="368" mass="41185" checksum="A02A8F158D6A59CB" modified="2010-06-15" version="1">MSLKRSAPLDLSPSSPASPPPPKRQHHKPAPLSSPHNPLQQPPSKLLPSAPTPEEDDYMNMTIPDPTPTGPESYTERLKRKQRAAELAQPKSKAELARIGGRSRDEALTKSLLWHESAGANKGLKMMKKMGFTPGSGLGRKEWENGGDGARLEPIGVSMKSDRAGIGLEKATGEKMKDVAAGIGVEQLNPEEYRERVRIQAREARMEAQFFSAQKVCEKFEDTEDNLGGNAQRSVPLKSINVFWRGLVKAREERERGRKMKHEMLQSLDLPRLPGYDRNHELDHEDKVALGIAPGRIRNFGMMVEDGAEYETDEDEELAEFEALEVGERLKRIVAHLRSKHNYCFWCKYQYPDGEMEGCPGLGEDLHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGX7</accession>
    <name>D5GGX7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007602001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430297">
        <property type="protein sequence ID" value="CAZ83770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839579.1">
        <property type="nucleotide sequence ID" value="XM_002839533.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGX7"/>
    <dbReference type="STRING" id="656061.D5GGX7"/>
    <dbReference type="GeneID" id="9181741"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007602001"/>
    <dbReference type="eggNOG" id="ENOG502SICU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034353_1_1_1"/>
    <dbReference type="InParanoid" id="D5GGX7"/>
    <dbReference type="OMA" id="WFMAGNE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072779" evidence="1">
        <location>
            <begin position="30"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="391" mass="43033" checksum="A57455B9F0511914" modified="2010-06-15" version="1">MFYQSLLQSLLFSPFLPFLLLSSPAVTTAEKLDEAAFKLKESGYNSHIATLSQLLPGASVLDVLAATNHDMVAEEVHAVGMSYKATRWENSASFNDFGTTSWYPQGVSTSADAYDVGTYEGKRVTLVSWYDNRDVPAAENKGVRIAFVDRDKSPDAYRYVLLVEPYLNAGTADFRAVNVHAGGIAWYGNSLYIVDTNKGFRVFDLTQIWKVETGDGIGRKSSGGFSAANYAYVLPQSRTYTQTNPPTPSVRFSFVSLDRTTTPDSLLVGEYQPASDTGPRRFIRWSIDYTNRQLITDSAGVATATWAYEVNIDRMQGVNSVNGKFYISRSNGRSNGDFISWVPGSTAKIAANAVPEGPEDLSYDKTTGLMYTVTEAVNGRYILPFDPKILP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHA4</accession>
    <name>D5GHA4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00007719001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430308">
        <property type="protein sequence ID" value="CAZ83856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839665.1">
        <property type="nucleotide sequence ID" value="XM_002839619.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHA4"/>
    <dbReference type="FunCoup" id="D5GHA4">
        <property type="interactions" value="896"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GHA4"/>
    <dbReference type="GeneID" id="9182561"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007719001"/>
    <dbReference type="eggNOG" id="KOG0394">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_10_6_1"/>
    <dbReference type="InParanoid" id="D5GHA4"/>
    <dbReference type="OMA" id="FSHINSW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032889">
        <property type="term" value="P:regulation of vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01862">
        <property type="entry name" value="Rab7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000086">
        <property type="entry name" value="Ras-related small GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47981">
        <property type="entry name" value="RAB FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47981:SF20">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB-7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ83856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="202" mass="22454" checksum="8C8A5C1A9308F411" modified="2010-06-15" version="1">MATRKKFLKVIILGDSGVGKTSLMNQYVNKVFSVSYKATIGADFLTKDVMVDDRLVTLTLWDTAGQERFQSLGVAFYRGADCCVLVYDVNNAKSFDNLNSWRDEFLIQASPRDPDTFPFVVLGNKIDVDESKRRVSNKRAQAYCLSKGQLPHFECSAKDGANVEVAFEAIARQALAQDESEIDYFDPTVNTGFPIESDGCSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHP5</accession>
    <name>D5GHP5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00008077001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430320">
        <property type="protein sequence ID" value="CAZ84075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839884.1">
        <property type="nucleotide sequence ID" value="XM_002839838.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHP5"/>
    <dbReference type="SMR" id="D5GHP5"/>
    <dbReference type="FunCoup" id="D5GHP5">
        <property type="interactions" value="93"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GHP5"/>
    <dbReference type="GeneID" id="9183729"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008077001"/>
    <dbReference type="eggNOG" id="KOG0118">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017390_0_0_1"/>
    <dbReference type="InParanoid" id="D5GHP5"/>
    <dbReference type="OMA" id="HIRYIAD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051252">
        <property type="term" value="P:regulation of RNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12520">
        <property type="entry name" value="RRM1_MRN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12523">
        <property type="entry name" value="RRM2_MRN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12521">
        <property type="entry name" value="RRM3_MRN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12522">
        <property type="entry name" value="RRM4_MRN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000047">
        <property type="entry name" value="Differentiation 1 negative regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000064">
        <property type="entry name" value="Differentiation 1 negative regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000130">
        <property type="entry name" value="Differentiation 1 negative regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000120">
        <property type="entry name" value="Negative regulator of differentiation 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039171">
        <property type="entry name" value="Cwc2/Slt11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034195">
        <property type="entry name" value="Mrn1_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR RBM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089:SF8">
        <property type="entry name" value="RNA-BINDING PROTEIN MRN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="134"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="225"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="359"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="450"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="518"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="527"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="559"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="582"/>
            <end position="603"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ84075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="734" mass="77797" checksum="5A59AB95D88B33CF" modified="2010-06-15" version="1">MSMPAGFSTDSVNHPNRRRINMPSINTGAPHGGDNGMASPGTGYDMQFTQLIPSHLLSPFQPGTPNAFASPNFPMHGFQQTPTSGNPLSPTFGANGLGSPTGFNQQYATYYQQSSPTATSPLAATPGMIAGTSRTVYLGNIPPETSAEEILGHVRSGQIESVRLLPDKNCAFISFLDSSSANHFHSDAILKKLSIRGQDVKVGWGKPSQVPTSVALAVQQSGASRNVYLGNLPEDVSEEELREDLGKFGPIDTVKIVREKAIGFVHFLSIGNAIKAVSQLPQEAKWQSPRRVYYGKDRCAYVSKTQQQNAAQYLGIAPGYAHVLNGADREMISSALAQQSVAAAAVATSAGGMNNLGNRTVYLGNIHPETTIEEICNVVRGGLLHHIRYIPDKHICFVTFIDPTAAASFYALSNLQGLMIHNRRLKIGWGKHSGALPPAIALAVSGGASRNVYVGNLDESWSEERLRQDFSEYGEIELVNALREKSCAFVNFTNIANAIKAIEAVRGREEYKRFKINFGKDRCGNPPRQTTNNHQSGTPTSAGHPPLPSTSPVNGPPSGGLQPSLSQSGSGGSPAQLSPSGMHNSNSPNNVGTPTTPNVQHMLNNGAAANPLQAYLSQQQQQQEAAMYASNLQQQLAAAQAAAVYGQPSGSQLDPSGQAATAAVEGLGQTHNGANGLGLAGMGIQNQNLRVGGQHNRALSMPVLGQQNQIGLGVNGYNGMGNGIGWAEEDVNMH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHS8</accession>
    <name>D5GHS8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mediator of RNA polymerase II transcription subunit 9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Mediator complex subunit 9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MED9</name>
        <name evidence="11" type="ORF">GSTUM_00008019001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 9">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Mediator complex subunit 9 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430320">
        <property type="protein sequence ID" value="CAZ84039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839848.1">
        <property type="nucleotide sequence ID" value="XM_002839802.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHS8"/>
    <dbReference type="STRING" id="656061.D5GHS8"/>
    <dbReference type="GeneID" id="9183693"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008019001"/>
    <dbReference type="eggNOG" id="ENOG502SW8C">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097220_3_0_1"/>
    <dbReference type="InParanoid" id="D5GHS8"/>
    <dbReference type="OMA" id="VEMQIVK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037212">
        <property type="entry name" value="Med7/Med21-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011425">
        <property type="entry name" value="Med9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07544">
        <property type="entry name" value="Med9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140718">
        <property type="entry name" value="Mediator hinge subcomplex-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0010">Activator</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="67"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ84039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="115" mass="13296" checksum="C5011D55A609677E" modified="2010-06-15" version="1">MTNEPPSIATSNLPPEFLPANAFDFIPDLHALLLRVSREELELKDVDHESSHIRLKMEKARAMVANLPDVDRSLEEQKKEIRELEERIEKQRGMIKAVVEMQIVKDIIRRRGGVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5GI08</accession>
    <name>D5GI08_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">alpha-1,2-Mannosidase</fullName>
            <ecNumber evidence="14">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">GSTUM_00008220001</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N(4)-(alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 9A1,2,3B1,2,3) + 4 H2O = N(4)-(alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2) + 4 beta-D-mannose</text>
            <dbReference type="Rhea" id="RHEA:56008"/>
            <dbReference type="Rhea" id="RHEA-COMP:14356"/>
            <dbReference type="Rhea" id="RHEA-COMP:14367"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28563"/>
            <dbReference type="ChEBI" id="CHEBI:59087"/>
            <dbReference type="ChEBI" id="CHEBI:139493"/>
            <dbReference type="EC" id="3.2.1.113"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N(4)-(alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 8A1,2,3B1,3) + 3 H2O = N(4)-(alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2) + 3 beta-D-mannose</text>
            <dbReference type="Rhea" id="RHEA:56028"/>
            <dbReference type="Rhea" id="RHEA-COMP:14358"/>
            <dbReference type="Rhea" id="RHEA-COMP:14367"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28563"/>
            <dbReference type="ChEBI" id="CHEBI:59087"/>
            <dbReference type="ChEBI" id="CHEBI:60628"/>
            <dbReference type="EC" id="3.2.1.113"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the glycosyl hydrolase 47 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="14"/>
    <dbReference type="EMBL" id="FN430322">
        <property type="protein sequence ID" value="CAZ84151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839960.1">
        <property type="nucleotide sequence ID" value="XM_002839914.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GI08"/>
    <dbReference type="STRING" id="656061.D5GI08"/>
    <dbReference type="GeneID" id="9184236"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008220001"/>
    <dbReference type="eggNOG" id="KOG2431">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003818_0_2_1"/>
    <dbReference type="InParanoid" id="D5GI08"/>
    <dbReference type="OMA" id="PESFGWD"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004571">
        <property type="term" value="F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000047">
        <property type="entry name" value="Mannosyl-oligosaccharide alpha-1,2-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001382">
        <property type="entry name" value="Glyco_hydro_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050749">
        <property type="entry name" value="Glycosyl_Hydrolase_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036026">
        <property type="entry name" value="Seven-hairpin_glycosidases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742:SF101">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE ALPHA-1,2-MANNOSIDASE 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE ALPHA-1,2-MANNOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01532">
        <property type="entry name" value="Glyco_hydro_47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00747">
        <property type="entry name" value="GLYHDRLASE47"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48225">
        <property type="entry name" value="Seven-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0106">Calcium</keyword>
    <keyword evidence="6 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 15" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072804" description="alpha-1,2-Mannosidase" evidence="15">
        <location>
            <begin position="20"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="118"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="264"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="373"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="407"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="499"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="330"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAZ84151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="508" mass="56305" checksum="B4776081918530A0" modified="2010-06-15" version="1">MHISRTLLTALLIAATTLGAPNIPLEARKRQATNARTDAVKEAFLHSWNGYTKFAFGFDELSPVSNKGTNSRNGWGASIVDAMSTAVLMELDDVVLKQLEHIKTVDFTNTATTVSLFETTIRYLGGLLSGYDLLMGPYKDLLPSNSTTLISSLVDQAVILADALAFGFDTASGVPANNLNFANKSFTDTTNGIATTGTLVLEWTRLADITGNRKYAELSQKAESYLLRPTPSLGEPFPGLLGTNVDIKTGQFIDSRGGWGGGTDSFYEYLIKMWVYDEEKYGLYKERWIAAADSTIKHLTSHPQPRQDITFVAEYQNAQTLSLNQGHLTCFIGGNFILGGQVLSRKEYTDYGLALTSGCHHTYDSTPSSIGPERWSWDPRNVPAAQSDFYARNGFYTTASYYDLRPEVIESYYHAFVATGDEKYREWAWDAFLAINATARTASGFTAVSDVGQENGGSKFDNQESFWFAEVLKYLYLIFQEDGKVGFVKGAEQKWVFNTEAHPFKVRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GI75</accession>
    <name>D5GI75_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00008318001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430324">
        <property type="protein sequence ID" value="CAZ84218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840027.1">
        <property type="nucleotide sequence ID" value="XM_002839981.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GI75"/>
    <dbReference type="GeneID" id="9182357"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008318001"/>
    <dbReference type="HOGENOM" id="CLU_2924399_0_0_1"/>
    <dbReference type="InParanoid" id="D5GI75"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ84218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="61" mass="6576" checksum="F1F0AF437685C82D" modified="2010-06-15" version="1">MPVTDPSCSSPVGSMTPLKTLPSAYFLPNHFKTPLAFPSFSFFSTSTSFTSSTFSLSSFAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5GI79</accession>
    <name>D5GI79_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00008323001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430324">
        <property type="protein sequence ID" value="CAZ84222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840031.1">
        <property type="nucleotide sequence ID" value="XM_002839985.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GI79"/>
    <dbReference type="FunCoup" id="D5GI79">
        <property type="interactions" value="26"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GI79"/>
    <dbReference type="GeneID" id="9181940"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008323001"/>
    <dbReference type="eggNOG" id="KOG0235">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033323_13_0_1"/>
    <dbReference type="InParanoid" id="D5GI79"/>
    <dbReference type="OMA" id="GWLIWRD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050278">
        <property type="term" value="F:sedoheptulose-bisphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046390">
        <property type="term" value="P:ribose phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050275">
        <property type="entry name" value="PGM_Phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48100">
        <property type="entry name" value="BROAD-SPECIFICITY PHOSPHATASE YOR283W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48100:SF15">
        <property type="entry name" value="SEDOHEPTULOSE 1,7-BISPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="1">
        <location>
            <position position="11"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="92"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="23"/>
            <end position="24"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="92"/>
            <end position="95"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ84222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="232" mass="25573" checksum="99852C65570EFC5A" modified="2010-06-15" version="1">MPTPRLFLARHGETAWTLTGQHTGVSEIPLTPHGETQVTLSSAALIGPSKHIDPANLTKIYISPRIRARRTAELLIPDTRATEVIFDERVREWGYGRYEGLRPKEILADRRRRGLVVEGFSIWKDGCEDSVDGFGIGKGESPKGIEARIDEIIKEIRGIQGGGIEAGRGDVDILIVSHGHYLRAFTKRWLGFSLDFPLTMVLEPGGIATLTYAHNNVNEPALLLGGTFRPGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIF1</accession>
    <name>D5GIF1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Myosin-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Class I unconventional myosin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Type I myosin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">GSTUM_00008458001</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Actin patch</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 18">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430326">
        <property type="protein sequence ID" value="CAZ84294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840103.1">
        <property type="nucleotide sequence ID" value="XM_002840057.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIF1"/>
    <dbReference type="FunCoup" id="D5GIF1">
        <property type="interactions" value="300"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GIF1"/>
    <dbReference type="GeneID" id="9184652"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008458001"/>
    <dbReference type="eggNOG" id="KOG0162">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000192_7_6_1"/>
    <dbReference type="InParanoid" id="D5GIF1"/>
    <dbReference type="OMA" id="NDQENQC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030428">
        <property type="term" value="C:cell septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001411">
        <property type="term" value="C:hyphal tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051666">
        <property type="term" value="P:actin cortical patch localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01378">
        <property type="entry name" value="MYSc_Myo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11858">
        <property type="entry name" value="SH3_Myosin-I_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.720:FF:000015">
        <property type="entry name" value="Myosin I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.4820:FF:000004">
        <property type="entry name" value="Myosin IE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.530:FF:000007">
        <property type="entry name" value="Myosin IE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035535">
        <property type="entry name" value="Fungal_myosin-I_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010926">
        <property type="entry name" value="Myosin_TH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036072">
        <property type="entry name" value="MYSc_Myo1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF837">
        <property type="entry name" value="MYOSIN-3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06017">
        <property type="entry name" value="Myosin_TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51757">
        <property type="entry name" value="TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 18" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="10 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 18" id="KW-0505">Motor protein</keyword>
    <keyword evidence="11 18" id="KW-0518">Myosin</keyword>
    <keyword evidence="8 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 17" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="Myosin motor" evidence="21">
        <location>
            <begin position="42"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="domain" description="TH1" evidence="22">
        <location>
            <begin position="779"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="20">
        <location>
            <begin position="1052"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="18">
        <location>
            <begin position="594"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="958"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1104"/>
            <end position="1209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="990"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="19">
        <location>
            <begin position="1037"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="19">
        <location>
            <begin position="1113"/>
            <end position="1125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="1126"/>
            <end position="1139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="1163"/>
            <end position="1184"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="135"/>
            <end position="142"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00029665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00032645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="CAZ84294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1209" mass="133592" checksum="5F69E635A4CB3ADF" modified="2010-06-15" version="1">MILTAPTKRAGRKAKNKDAPTSSGGQPRVKKAVFETTKKKEVGVTDLTLISKISNEAINENLKKRFENREIYTYIGHVLISVNPFRDLGIYTDLVLASYRGKNRLEVPPHVFAVAESAYYNMNGYKENQCIIISGESGAGKTEAAKRIMQYIAAASSTSSTTIQEIKDMVLATNPLLESFGCAKTLRNNNSSRHGKYLEIQFNSQGEPVGANITNYLLEKTRVVEQIRDERNFHIFYQFTKAASQNYRETFGLQTPDTYLYTSKSKCLDVPGIDDVAEFQDTLSAMRVIGLSQGEQDQIFRMLAAILWLGNIQFVEDEQGNGAISDSSVTDFVAYLLEVDGAAVHKALTLRMIETSRGGRRGSVYEVPLNPTQATAVRDALGKAIYQNLFEWIVDRVNISMKTRGAIAHTIGILDIYGFEIFENNSFEQICINYVNEKLQQIFIQLTLRTEQEEYAREQITWTPIKYFDNRIVCDLIEEKRPPGVFAALNDACATAHADSGAADGTFVQRLNMLASNPHFENRQGQFLVKHYAGDVMYTVAGMTDKNKDQLLKDLLDLVGQSGNAFLRTLFPNQVDRDSKRRPPTASDKIKASANDLVTTLMKAQPSYIRTIKPNQNKSPTEYDVPAVLHQIKYLGLQENVRIRRAGFAYRQTFEKFIERFYLLSPKTSYAGDYTWTGDARTGTLQILKDTSIPKEEYQMGVSKAFIKTPETLFALETMRDRYWHNMAIRIQRAWRNYLRYRAECATRIQRAWRKKRGGEEFLKIRDQGHKLLGGRKERRRMSLLGSRRFMGDYLGVANVGSRLEPIRAPAGIGLGEKVLFSCRSKVLVSKLGRSSKPSPRILVMTNKAIYIVIQAMANGQVAISSERTMPLGSIKFVSMSSLRDDWFSLGVGSPQQPDTLVSCVFKTELVTHLRAAMPGGLDLRIGPVIEYAKKPGKMAAVKVVKDLAIPRDDLYKSSTIHVGPGEPPNSISRPTPKGKPVAARPVTSGKLLKPGGPRGGPARNKNPPSMTPAPNANASALLPVPPKSVVPEASRIPPPAPPAPPAAPPRVADPTCRALYDFAGQTENELSLSKDEVVIIIRKEANGWWLTKKMNSTEQGWAPSAYLEEEAAPPPTPPPPPVAPARPANGAARPAGRKPAPPAPPAKRPTVGRKPATVENRSSVAGSTSSSSSGAGSSTTSFAPGLAQALLDRQQAMRGGRKDESDDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIG1</accession>
    <name>D5GIG1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00008485001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430326">
        <property type="protein sequence ID" value="CAZ84304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIG1"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008485001"/>
    <dbReference type="HOGENOM" id="CLU_2689597_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIG1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ84304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="74" mass="7956" checksum="5FF3F8C2DC3CB82A" modified="2010-06-15" version="1">MDGIFSLLKYDSRELIVGGGQLGAALVVEEVGSMEFAPIPCLRVILLVGLAGDREPREGFMNSHPVEEFCCGES</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GII2</accession>
    <name>D5GII2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">cyclic pyranopterin monophosphate synthase</fullName>
            <ecNumber evidence="3">4.6.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00008511001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate = cyclic pyranopterin phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:49580"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:59648"/>
            <dbReference type="ChEBI" id="CHEBI:131766"/>
            <dbReference type="EC" id="4.6.1.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.17" evidence="3"/>
    <dbReference type="EMBL" id="FN430327">
        <property type="protein sequence ID" value="CAZ84325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840134.1">
        <property type="nucleotide sequence ID" value="XM_002840088.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GII2"/>
    <dbReference type="STRING" id="656061.D5GII2"/>
    <dbReference type="GeneID" id="9182341"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008511001"/>
    <dbReference type="eggNOG" id="KOG2876">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074693_1_0_1"/>
    <dbReference type="InParanoid" id="D5GII2"/>
    <dbReference type="OMA" id="HELSTHV"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061799">
        <property type="term" value="F:cyclic pyranopterin monophosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061798">
        <property type="term" value="F:GTP 3',8'-cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01420">
        <property type="entry name" value="MoaC_PE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.640">
        <property type="entry name" value="Molybdopterin cofactor biosynthesis C (MoaC) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023045">
        <property type="entry name" value="MoaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047594">
        <property type="entry name" value="MoaC_bact/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036522">
        <property type="entry name" value="MoaC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050105">
        <property type="entry name" value="MoCo_biosynth_MoaA/MoaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002820">
        <property type="entry name" value="Mopterin_CF_biosynth-C_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00581">
        <property type="entry name" value="moaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006870">
        <property type="entry name" value="PRK09364.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22960:SF0">
        <property type="entry name" value="MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22960">
        <property type="entry name" value="MOLYBDOPTERIN COFACTOR SYNTHESIS PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01967">
        <property type="entry name" value="MoaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55040">
        <property type="entry name" value="Molybdenum cofactor biosynthesis protein C, MoaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Molybdopterin cofactor biosynthesis C (MoaC)" evidence="7">
        <location>
            <begin position="100"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="32"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="60"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="72"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ84325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="254" mass="26729" checksum="358FFE8D08303234" modified="2010-06-15" version="1">MNRKFLSSSGVSKAHIPGVIFPFITPSQSLTHTQPIRAYSASSTEQPSPPSPPTKNDNPNSPPTPVPPLLPSPFSAHQPPSSSSSPQTLTHIAPTGEAHMVSISAKPPTRRASTARGRVVFSNPTARDLILKGLNKKGDVLSVARVAGIMAVKKTPDVIPLCHPILIDGVDVRVELGGHGGGGETEEREGNGYVDICVTVRCEGKTGVEMEALTGVSVAALTVFDMCKAVDKRMVIEGVRVVRKSGGRSGDFVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>D5GII6</accession>
    <name>D5GII6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00008517001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430327">
        <property type="protein sequence ID" value="CAZ84329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840138.1">
        <property type="nucleotide sequence ID" value="XM_002840092.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GII6"/>
    <dbReference type="GeneID" id="9183263"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008517001"/>
    <dbReference type="eggNOG" id="ENOG502S0SQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015101_4_2_1"/>
    <dbReference type="InParanoid" id="D5GII6"/>
    <dbReference type="OMA" id="PNWVGFL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01846">
        <property type="entry name" value="fatty_acyltransferase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051058">
        <property type="entry name" value="GDSL_Est/Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45648">
        <property type="entry name" value="GDSL LIPASE/ACYLHYDROLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_4G14700)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45648:SF22">
        <property type="entry name" value="GDSL LIPASE_ACYLHYDROLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_4G14700)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072177" evidence="2">
        <location>
            <begin position="20"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ84329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="325" mass="36741" checksum="991E05F975C97B84" modified="2010-06-15" version="1">MFSRLSIISLGLADALCGAIPFVEEGYNEHVYPGWDQLKYLFVFGDSYTTTGFNLTNGSPLPSSGNPMGNPAWPGENSGPNWVGYLTTTFNRSELLTYNLAYGGATINSTLVKPYIPTVLSFADQVQDLFLPNLSPKSPTSITPAWESEDSLFLIWLGVNDIENSYRNVDPGDLHPSLMNEYFRILEELYCVGARNFLLLNVPPIDRSPLTTGQGEQAVSREQEALESYNSLLEDMLHGFKRSHRDVYGKIFDAARVFTEILERPERYDIKNTTAFCEKYRVGTPDWDTYYPECGVRVSEYFWLNSLHPTWPVHKVLAEEIVKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIK8</accession>
    <name>D5GIK8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Tubulin beta chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">GSTUM_00008543001</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 13">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4 13">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430327">
        <property type="protein sequence ID" value="CAZ84351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840160.1">
        <property type="nucleotide sequence ID" value="XM_002840114.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIK8"/>
    <dbReference type="FunCoup" id="D5GIK8">
        <property type="interactions" value="1193"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GIK8"/>
    <dbReference type="GeneID" id="9183285"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008543001"/>
    <dbReference type="eggNOG" id="KOG1375">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015718_1_1_1"/>
    <dbReference type="InParanoid" id="D5GIK8"/>
    <dbReference type="OMA" id="CQDEMEG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000070">
        <property type="term" value="P:mitotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097435">
        <property type="term" value="P:supramolecular fiber organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02187">
        <property type="entry name" value="beta_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000002">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000002">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000009">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013838">
        <property type="entry name" value="Beta-tubulin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002453">
        <property type="entry name" value="Beta_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01163">
        <property type="entry name" value="BETATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00228">
        <property type="entry name" value="TUBULIN_B_AUTOREG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10 13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 13" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="15">
        <location>
            <begin position="47"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="16">
        <location>
            <begin position="246"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="425"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="430"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAZ84351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="447" mass="49526" checksum="A44F2491A92C5620" modified="2010-06-15" version="1">MREIIHLQTGQCGNQIGTAFWQTIGGEHGLDGSGVYGGTSDIQLERMSVYFNEASGSKYVPRAVLVDLEPGTMDAVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDQVLDVVRREAEGCDCLQGFQITHSLGGGTGAGMGTLLISKIREEFPDRMMSTFSVVPSPKVSDTVVEPYNATLSIHQLVENSDETFCIDNEALYDICMRTLKLAAPSYGDLNHLVSAVMSGVTTCLRFPGQLNSDLRKLAVNMVPFPRLHFFMVGFAPLTSRGAHSFRAATVPELTQQMFDPRNMMAAPDFRNGRYLTCATIFRGKVSMKEVEDQMRNIQTKSSSYFVEWIPNNVQTALCSIPPKGLKMSATFIGNSTSIQEMFKRIGDQFTVMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVAEYQQYQDAGISDDEGEAYEEELPADGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIX4</accession>
    <name>D5GIX4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Peroxisomal membrane protein PEX13</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Peroxin-13</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">GSTUM_00008726001</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Mel28</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="13">Interacts (via SH3 domain) with PEX14 (via SH3-binding motif); forming the PEX13-PEX14 docking complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peroxin-13 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840276.1">
        <property type="nucleotide sequence ID" value="XM_002840230.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIX4"/>
    <dbReference type="FunCoup" id="D5GIX4">
        <property type="interactions" value="154"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GIX4"/>
    <dbReference type="GeneID" id="9185685"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008726001"/>
    <dbReference type="eggNOG" id="KOG3875">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034386_1_2_1"/>
    <dbReference type="InParanoid" id="D5GIX4"/>
    <dbReference type="OMA" id="EGWFPKK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990429">
        <property type="term" value="C:peroxisomal importomer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016560">
        <property type="term" value="P:protein import into peroxisome matrix, docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11771">
        <property type="entry name" value="SH3_Pex13p_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000128">
        <property type="entry name" value="Peroxisomal membrane protein (Pex13)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007223">
        <property type="entry name" value="Peroxin-13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035463">
        <property type="entry name" value="Pex13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19332">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19332:SF1">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04088">
        <property type="entry name" value="Peroxin-13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="265"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="17">
        <location>
            <begin position="312"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="31"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="42"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00065871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAZ84467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="427" mass="44864" checksum="C45E09806A46E251" modified="2010-06-15" version="1">MSQISPPKPWEVNGLQQSSLGSSSLSIMSTTAAAPVPPPAPATTAAPAMAAPGATLSAPPSLPARPSALTGAMTTNRLGATTTAAGAYSAYNRPGYGLGGGLGGGYGSYGAGMSPYSRFGGYGGGMYGGMGGFGGYGGFGGMPGDPNDPNSFARGMESSTAATFQMIESIVGAFGGFAQMLESTYMATHSSFFAMVQVAEQFGNLRNTLGSILGIFTMMRWARIAVAKVTGRPLPASAGELTASNFAAFDSRGGKGSSRPSKKPFLFFVFAVFGLPYLMGKLIRSLARREEERKKIMGTIPEGDVAAQMDPTKLEFCRVTFDFTPEQNNGVELEVKKGDLVAVLSKADPMGNPSQWWKCRARDSRVGYLPSPYLEIIARKEGAAKQITAGDESRTQTMTSEIAGELLKDTKPEDISIESFQKSQFFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIZ9</accession>
    <name>D5GIZ9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ubiquinone biosynthesis monooxygenase COQ6, mitochondrial</fullName>
            <ecNumber evidence="11">1.14.15.45</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">2-methoxy-6-polyprenolphenol 4-hydroxylase</fullName>
            <ecNumber evidence="11">1.14.15.46</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">COQ6</name>
        <name evidence="13" type="ORF">GSTUM_00008757001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">FAD-dependent monooxygenase required for two non-consecutive steps during ubiquinone biosynthesis. Required for the C5-ring hydroxylation during ubiquinone biosynthesis by catalyzing the hydroxylation of 4-hydroxy-3-(all-trans-polyprenyl)benzoic acid to 3,4-dihydroxy-5-(all-trans-polyprenyl)benzoic acid. Also acts downstream of coq4, for the C1-hydroxylation during ubiquinone biosynthesis by catalyzing the hydroxylation of 2-methoxy-6-(all-trans-polyprenyl)phenol to 2-methoxy-6-(all-trans-polyprenyl)benzene-1,4-diol. The electrons required for the hydroxylation reaction are funneled indirectly to coq6 from NADPH via a ferredoxin/ferredoxin reductase system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 2-methoxy-6-(all-trans-polyprenyl)phenol + 2 reduced [2Fe-2S]-[ferredoxin] + O2 + 2 H(+) = a 2-methoxy-6-(all-trans-polyprenyl)benzene-1,4-diol + 2 oxidized [2Fe-2S]-[ferredoxin] + H2O</text>
            <dbReference type="Rhea" id="RHEA:81183"/>
            <dbReference type="Rhea" id="RHEA-COMP:9551"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:10858"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:62731"/>
            <dbReference type="ChEBI" id="CHEBI:84166"/>
            <dbReference type="EC" id="1.14.15.46"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 4-hydroxy-3-(all-trans-polyprenyl)benzoate + 2 reduced [2Fe-2S]-[ferredoxin] + O2 + 2 H(+) = a 3,4-dihydroxy-5-(all-trans-polyprenyl)benzoate + 2 oxidized [2Fe-2S]-[ferredoxin] + H2O</text>
            <dbReference type="Rhea" id="RHEA:81195"/>
            <dbReference type="Rhea" id="RHEA-COMP:9514"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:10930"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:64694"/>
            <dbReference type="ChEBI" id="CHEBI:78396"/>
            <dbReference type="EC" id="1.14.15.45"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="11">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of a multi-subunit COQ enzyme complex, composed of at least COQ3, COQ4, COQ5, COQ6, COQ7 and COQ9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Mitochondrion inner membrane</location>
            <topology evidence="11">Peripheral membrane protein</topology>
            <orientation evidence="11">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the UbiH/COQ6 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.15.45" evidence="11"/>
    <dbReference type="EC" id="1.14.15.46" evidence="11"/>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840301.1">
        <property type="nucleotide sequence ID" value="XM_002840255.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIZ9"/>
    <dbReference type="FunCoup" id="D5GIZ9">
        <property type="interactions" value="381"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GIZ9"/>
    <dbReference type="GeneID" id="9181833"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008757001"/>
    <dbReference type="eggNOG" id="KOG3855">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009665_8_0_1"/>
    <dbReference type="InParanoid" id="D5GIZ9"/>
    <dbReference type="OMA" id="VKQMQVW"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031314">
        <property type="term" value="C:extrinsic component of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120538">
        <property type="term" value="F:2-methoxy-6-polyprenolphenol 4-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106364">
        <property type="term" value="F:4-hydroxy-3-all-trans-polyprenylbenzoate oxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000021">
        <property type="entry name" value="Ubiquinone biosynthesis monooxygenase COQ6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000245">
        <property type="entry name" value="Ubiquinone biosynthesis monooxygenase COQ6, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03193">
        <property type="entry name" value="COQ6_monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018168">
        <property type="entry name" value="Ubi_Hdrlase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010971">
        <property type="entry name" value="UbiH/COQ6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051205">
        <property type="entry name" value="UbiH/COQ6_monooxygenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000689">
        <property type="entry name" value="UbQ_mOase_COQ6"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01988">
        <property type="entry name" value="Ubi-OHases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43876">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS MONOOXYGENASE COQ6, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43876:SF7">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS MONOOXYGENASE COQ6, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01304">
        <property type="entry name" value="UBIH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0274">FAD</keyword>
    <keyword evidence="3 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8 11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="domain" description="FAD-binding" evidence="12">
        <location>
            <begin position="47"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ84492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="487" mass="53211" checksum="CC8D31FD1D71D8D4" modified="2010-06-15" version="1">MSPIWCLRSRAAVPHLRSLTSQPQFIAFRHSSVPRTYATTNIPENLYDVVIVGGGPAGLSLATSLRSSSVTSRLRVALIEGLDLDAGRKWKPSSENYSNRVSSLTPCSVGFLTDIGVWKHVQRNRVQDYHGMQVWDGVSGSRIVFDWGAENSSTIAYMTENVNLVHGLLSRLGELGGAQLIDKTRVEGIYFGGDHGTLDLRTWPIVQITGGRELAARLLVGADGANSPVRAFAGIESRGWDYGRHGVVATVKIEDMPDVERTAYQRFLPTGPVALLPLPGNFATLVWSTTPEIAARLKKLSPVDFVAMVNSAFRLSHVDIAYMAKMDGGILEEYQWRQSVTPSDKAEVPSKILEVQENSIASFPLKMRHVDSYISERVALVGDAAHTIHPLAGQGLNQGIGDAQFLIKALETAVQNGQDIGSTLSLEPYCSDQYFKNNLMIGIVDKLHKLYGTAFPPVVAVRSLGLRAVNAMERLKSMIMKQAAGER</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJ24</accession>
    <name>D5GJ24_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00008788001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM14 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840326.1">
        <property type="nucleotide sequence ID" value="XM_002840280.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJ24"/>
    <dbReference type="FunCoup" id="D5GJ24">
        <property type="interactions" value="64"/>
    </dbReference>
    <dbReference type="GeneID" id="9181858"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008788001"/>
    <dbReference type="eggNOG" id="ENOG502S7UZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096652_3_0_1"/>
    <dbReference type="InParanoid" id="D5GJ24"/>
    <dbReference type="OMA" id="YGLFTFG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1740">
        <property type="entry name" value="Transmembrane protein 14-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005349">
        <property type="entry name" value="TMEM14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044890">
        <property type="entry name" value="TMEM14_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12668">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 14, 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12668:SF15">
        <property type="entry name" value="UPF0136 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_1G03720)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03647">
        <property type="entry name" value="Tmemb_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="87"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ84517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="112" mass="11450" checksum="5098804BF2FF6A7B" modified="2010-06-15" version="1">MIAKDLSAENKEALLIGSLCALGGTIGYAKTGSIPSITAGVTVGALYVCAGLRIRAEQSHGNEIAVLASLALAGSSAPRALRSGKGLPIGLSLLAAYGLVYYGIKVKQQHRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJH0</accession>
    <name>D5GJH0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00008995001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430331">
        <property type="protein sequence ID" value="CAZ84663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840472.1">
        <property type="nucleotide sequence ID" value="XM_002840426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJH0"/>
    <dbReference type="GeneID" id="9182664"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008995001"/>
    <dbReference type="eggNOG" id="KOG2533">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_0_1_1"/>
    <dbReference type="InParanoid" id="D5GJH0"/>
    <dbReference type="OMA" id="WVICIGM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000013">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000018">
        <property type="entry name" value="MFS transporter permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF53">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="102"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="165"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="208"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="269"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="309"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="340"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="366"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="400"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="432"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="40"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ84663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="490" mass="54721" checksum="C85282B4DBFBE294" modified="2010-06-15" version="1">MQGHSSAKEIHHENYEISSDGESWSAEEEACVRRRLDWRLLPLLTTLYMLCFLDRWVTDNARIQGMQHDLHLIGFRFNWALTMFYIPYLLVEIPSNILLNRIGILILMLFPALIIAFGIVSIGTAFVKSFQGLCVARAFLGFAEGGTMPGMAFVMSCFYKRGELLFRVGLLASAALLSGAFGGFLATALSRIPEWGAKGSEVHTWRNIFFFEGLITVGFGVLAYFFIPKSPQDCMFLNERERWIAAERMRIEHREITSEKTELWHVKRAVFNINNVTCCMGFFFCNITAQGFSLFLPTILSALGWRATKAQLLTVPPYTVAFCFSIYIARLSDRYRKRGLFILVCTTLTLTGYSMLVSVSSANIKYVAVFFVATGALPPGPAFLSWGLNNAAGPSIRAITGGYIVSVGTCGAILATWTYISSDAPKYTRGHSINLGGQACLALVALFGIFYTRRENALRDRGGRDGRLVGLAEGQARKLGYRHPEFRYVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJI7</accession>
    <name>D5GJI7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00009033001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Siderophore biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430332">
        <property type="protein sequence ID" value="CAZ84680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840489.1">
        <property type="nucleotide sequence ID" value="XM_002840443.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJI7"/>
    <dbReference type="STRING" id="656061.D5GJI7"/>
    <dbReference type="GeneID" id="9182681"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009033001"/>
    <dbReference type="eggNOG" id="KOG1680">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_7_6_1"/>
    <dbReference type="InParanoid" id="D5GJI7"/>
    <dbReference type="OMA" id="DPDLHWK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000074">
        <property type="entry name" value="Enoyl-CoA hydratase (AFU_orthologue AFUA_2G10650)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941:SF158">
        <property type="entry name" value="ENOYL-COA HYDRATASE (AFU_ORTHOLOGUE AFUA_2G10650)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941">
        <property type="entry name" value="ENOYL-COA HYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ84680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="276" mass="29305" checksum="025468D68CF8AA4D" modified="2010-06-15" version="1">MLRTPPPPAENLILSFPSLHILVVTINRPQAMNCVDTQTHNEMSRVWPWYDDEPSLRCAVVTGNPQGKRKAFCAGQDLKEWNDAQSGGADPIVAHPQGGFGGLSRRSGKKPVIAAVDGLALGGGMEMLANMDMVVASASSMLGLPEVKRGVAAFAGVLPRLTMSVGLQRASELALTGRMLSASEARGWGLVNRVVGDGEALVVAVAVAKEIAGNSPDSVIVSRAGVRSAWEGRSVEEATRDVREGVGMGLQTGENIREGLRAFVEKREPKWADSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJT1</accession>
    <name>D5GJT1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00009180001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430333">
        <property type="protein sequence ID" value="CAZ84774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_002840537.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJT1"/>
    <dbReference type="STRING" id="656061.D5GJT1"/>
    <dbReference type="GeneID" id="9186810"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009180001"/>
    <dbReference type="eggNOG" id="KOG4210">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026791_1_1_1"/>
    <dbReference type="InParanoid" id="D5GJT1"/>
    <dbReference type="OMA" id="EIRIVMN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050502">
        <property type="entry name" value="Euk_RNA-bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025">
        <property type="entry name" value="OS02G0815200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025:SF1">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="302"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="402"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="371"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="474"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="59"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="84"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="100"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="110"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="167"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="186"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="214"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="237"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="383"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="485"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ84774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="559" mass="58556" checksum="4F141FB5D0651B4E" modified="2010-06-15" version="1">MAKEKPFAKPTANGKAGKKDVLGSVKSGAITKKSTKSTNSKKLGQKTVSTVSGHKATGLNRSTQSTKNIEPKRKAKAPTPLSSESEDSSESESESGSGSDSDESGSESEVETKKPIKKAEVKKVGVKKVEVKRPEAGKIVAKRPERKEAVAKDSSDSESNASCSDGDGGDSDSDSDGEAPKAKTASETTSISSSGSDSDSSSDSSSDEEEAPVAKKAAPVKAVNGTKKTVGVAESSCESDSDSDSSSDSDARVKSTGKAVSDSESSDSSDSESDAKKRKAATEAEPTPKKVKTVSSAQEGAKNLFVGSLSWNVDEGWLRNEFEQFGEIAAVRVVTDRESGRSKGFGYVEYTTNEAAKKALEEMKGKDIDGRTINVDFSAPRPENPRQDRSRLYGDQKSPESETVFVANLSFEADEQIVQTEFEGFGNIVGLRIPTDPESGQPKGFCYIQYDRVDSARKAVEEMNGALVAGRAIRTDFSTPRDPSAGGGRGGGRGGRGGFSGRGGRGGGFTDRGGRGGSRGRGGRGDGRGRGGPGGGRGRFQSQNRGGFGDFQGKKQKFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJU5</accession>
    <name>D5GJU5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Beta-xylanase</fullName>
            <ecNumber evidence="10">3.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00009198001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>Endohydrolysis of (1-&gt;4)-beta-D-xylosidic linkages in xylans.</text>
            <dbReference type="EC" id="3.2.1.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Glycan degradation; xylan degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the glycosyl hydrolase 10 (cellulase F) family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.8" evidence="10"/>
    <dbReference type="EMBL" id="FN430333">
        <property type="protein sequence ID" value="CAZ84788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840597.1">
        <property type="nucleotide sequence ID" value="XM_002840551.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJU5"/>
    <dbReference type="STRING" id="656061.D5GJU5"/>
    <dbReference type="GeneID" id="9186824"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009198001"/>
    <dbReference type="eggNOG" id="ENOG502QR4K">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020161_5_0_1"/>
    <dbReference type="InParanoid" id="D5GJU5"/>
    <dbReference type="OMA" id="STREMWN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031176">
        <property type="term" value="F:endo-1,4-beta-xylanase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045493">
        <property type="term" value="P:xylan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044846">
        <property type="entry name" value="GH10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001000">
        <property type="entry name" value="GH10_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31490:SF35">
        <property type="entry name" value="ENDO-1,4-BETA-XYLANASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31490">
        <property type="entry name" value="GLYCOSYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00331">
        <property type="entry name" value="Glyco_hydro_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00134">
        <property type="entry name" value="GLHYDRLASE10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00633">
        <property type="entry name" value="Glyco_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51760">
        <property type="entry name" value="GH10_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 10" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0858">Xylan degradation</keyword>
    <feature type="domain" description="GH10" evidence="11">
        <location>
            <begin position="1"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ84788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="304" mass="33268" checksum="BBC67F915955DBA6" modified="2010-06-15" version="1">MEKLYFGSATDNPELTDAQYCTLLSNTKEFGQITPGNSMKWDATEGTRNTFTFAKDEAILALAERNGQKVRGHTLVWHSQLPISNGGFDNATLISIMENHITEVVKKWKGRILHWDVLNEIFNEDGTWRSSVFYNTIGEAFPGIAFRAARAADPSAKLYINDYNIDGTGAKSTAMYNLVKKLLAEGVPVDGIGVQAHLIVGNVPSTMQNNWARFASLGVDIAITELGIRMTFPADAAGLSQQADDYKNVATACINVPRCIGISRFHPYKYSWIPAIFPGQGAALPWDEDMVVKPAYAAIAGALT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5GK26</accession>
    <name>D5GK26_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00009333001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430335">
        <property type="protein sequence ID" value="CAZ84869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_002840632.1"/>
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    <dbReference type="AlphaFoldDB" id="D5GK26"/>
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        <property type="interactions" value="215"/>
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    <dbReference type="STRING" id="656061.D5GK26"/>
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    <dbReference type="KEGG" id="tml:GSTUM_00009333001"/>
    <dbReference type="eggNOG" id="KOG0132">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016577_0_0_1"/>
    <dbReference type="InParanoid" id="D5GK26"/>
    <dbReference type="OMA" id="GIVQTCI"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="P:sno(s)RNA 3'-end processing"/>
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        <property type="term" value="P:termination of RNA polymerase II transcription"/>
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    <dbReference type="CDD" id="cd16984">
        <property type="entry name" value="CID_Nrd1_like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="RNA binding protein Nrd1"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR006569">
        <property type="entry name" value="CID_dom"/>
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    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
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    <dbReference type="InterPro" id="IPR048892">
        <property type="entry name" value="Nrd1_Seb1_dom2"/>
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    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051485">
        <property type="entry name" value="SR-CTD_assoc_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23140:SF4">
        <property type="entry name" value="PROTEIN CBR-NRD-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23140">
        <property type="entry name" value="RNA PROCESSING PROTEIN LD23810P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04818">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21380">
        <property type="entry name" value="Nrd1-Seb1_dom2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00582">
        <property type="entry name" value="RPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51391">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="CID" evidence="8">
        <location>
            <begin position="1"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="504"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="188"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="224"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="394"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="631"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="229"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="248"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="263"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="404"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="657"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="674"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="708"/>
            <end position="733"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ84869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="733" mass="79389" checksum="8FBCD2597211BA10" modified="2010-06-15" version="1">MSAVVTELENALQAMQATKAPGVSGTKINQITELSLRNVQSESVIIQKVYTHFKKCPGTHKLGPLYVVDSIARKYLEQAKRLHEPVSHAAPDGTFGAGVHRITNLLPALMNDILQNAPPEENKDKIDKLVTIWERSSTFPPEVLADIKQRIAASPMSIVPNARSTTPTGSPPPHLQSLAGQIQNLPVYQPPQAHQNPQSAIPNTQSQNATTTAGILQALAQMAKQNKTAMPPQAPSQQQTPAPVPQYSAPTPQQQPSGMPNLSQSASSLVVSQPQQAQLPMAQMPYTSTPPNQNPNAAIPMMGNQLGGMFLGQPGQGQFPTFPPQPPLPPPIPQQGTAPAGIEQMALLQLLLQHGGMNLPQLTQSFGNLASGQGGPGADLGAVLPAWTQIMNAAQGQNQQDSSRSGDDGYGRGQRNREHYSPPSRSPPRYGRDARRGRSRSRSPNRFDRGSPRSRRDRSRTPPRFSNGKGQAERNVTPTALPKFPKHVEFDPTIGPNNIKVLSRTLFVGGVTITDDELRKLFEKHGYVQSCIVNQEKRHAFIKMLTRQDAVKARQGMETYRADNMTIRTRWGVGYGPRDCSDYQTGISVIPIDRLTDADQRWMVSAEYGGTGGRPLEGGMCVEEPDIEIGQGVSSKAISKRFPTDSGGAKGPRSSHDNTSSTGNASGGNTTQRDPPRTKRNDYSELHDRTGRDSNIGVAPPTPGFGTGLPFPMGMMPPNMPPGYQYPGFPNQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>D5GK41</accession>
    <name>D5GK41_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ATP-dependent 6-phosphofructokinase</fullName>
            <shortName evidence="14">ATP-PFK</shortName>
            <shortName evidence="14">Phosphofructokinase</shortName>
            <ecNumber evidence="14">2.7.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Phosphohexokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">GSTUM_00009355001</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14 15">
            <text>beta-D-fructose 6-phosphate + ATP = beta-D-fructose 1,6-bisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="14">Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 14 15">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade 'E' sub-subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.11" evidence="14"/>
    <dbReference type="EMBL" id="FN430335">
        <property type="protein sequence ID" value="CAZ84884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840693.1">
        <property type="nucleotide sequence ID" value="XM_002840647.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GK41"/>
    <dbReference type="FunCoup" id="D5GK41">
        <property type="interactions" value="869"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GK41"/>
    <dbReference type="GeneID" id="9186921"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009355001"/>
    <dbReference type="eggNOG" id="KOG2440">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011053_0_0_1"/>
    <dbReference type="InParanoid" id="D5GK41"/>
    <dbReference type="OMA" id="EWQDQMC"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00182"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005945">
        <property type="term" value="C:6-phosphofructokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003872">
        <property type="term" value="F:6-phosphofructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070095">
        <property type="term" value="F:fructose-6-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048029">
        <property type="term" value="F:monosaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061621">
        <property type="term" value="P:canonical glycolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030388">
        <property type="term" value="P:fructose 1,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070072">
        <property type="term" value="P:vacuolar proton-transporting V-type ATPase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.460:FF:000007">
        <property type="entry name" value="ATP-dependent 6-phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.460:FF:000008">
        <property type="entry name" value="ATP-dependent 6-phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.450">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.460">
        <property type="entry name" value="Phosphofructokinase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03184">
        <property type="entry name" value="Phosphofructokinase_I_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009161">
        <property type="entry name" value="6-Pfructokinase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022953">
        <property type="entry name" value="ATP_PFK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015912">
        <property type="entry name" value="Phosphofructokinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000023">
        <property type="entry name" value="Phosphofructokinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035966">
        <property type="entry name" value="PKF_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02478">
        <property type="entry name" value="6PF1K_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13697:SF4">
        <property type="entry name" value="ATP-DEPENDENT 6-PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13697">
        <property type="entry name" value="PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00365">
        <property type="entry name" value="PFK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000533">
        <property type="entry name" value="ATP_PFK_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00476">
        <property type="entry name" value="PHFRCTKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53784">
        <property type="entry name" value="Phosphofructokinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00433">
        <property type="entry name" value="PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 14" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="10 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 14 15" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="9 14 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphofructokinase" evidence="16">
        <location>
            <begin position="1"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphofructokinase" evidence="16">
        <location>
            <begin position="376"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-terminal catalytic PFK domain 1" evidence="14">
        <location>
            <begin position="1"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interdomain linker" evidence="14">
        <location>
            <begin position="362"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="C-terminal regulatory PFK domain 2" evidence="14">
        <location>
            <begin position="376"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="137"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="59"/>
            <end position="60"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="89"/>
            <end position="92"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14">
        <location>
            <begin position="135"/>
            <end position="137"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14">
        <location>
            <begin position="179"/>
            <end position="181"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14">
        <location>
            <begin position="269"/>
            <end position="272"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14">
        <location>
            <position position="446"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14">
        <location>
            <begin position="503"/>
            <end position="507"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="541"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14">
        <location>
            <begin position="548"/>
            <end position="550"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14">
        <location>
            <position position="608"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="634"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14">
        <location>
            <begin position="640"/>
            <end position="643"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14">
        <location>
            <position position="711"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR000533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAZ84884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="737" mass="80391" checksum="50F9597F43D13AAE" modified="2010-06-15" version="1">MNAAVRAVVRMSIAQGCEAYAIYEGYEGLVQGGGLIKRMHWEDVRGWLSEGGTLIGTARCKKFTERAGRLQGAKNLVLAGIDALVVCGGDGSLTGADIFRSEWPGLLAELIELGELAEEQCSPFKTLNIVGLVGSIDNDMSSTDATIGAYSSLTRICQAVDFIDATAYSHSRAFVVEVMGRHCGWLALMAGLSTGADFVFLPEKPPQDKWEGEMCRIIKTHRTAGKRKTIVIVAEGAIDRELKPITATYVRDILVDELRLDTRITTLGHVQRGGTACAFDRILATLQGGEAVRAVLESTPDTLSPMIAINENKITRKPLMDAVRLTKAVAECIKNKDFAGAMNLRDPEFEEYHRAYMVTTKPDELSEKAPQDKRMRIAIIHVGAPAGGMNAATRAAATFCLNRGHRPFAIHNGFPGLVRHDSVRELNWLTVEGWAIRGGSEIGTNRSLPSEDMGMVAWYFQRYKFDGLLIIGGFEAYHALTELHSARDSYPAFRIPMICLPATISNNVPGTEFSIGSDTCLNALIEYCDAIKQSASASRRRVFVVECQGGKSGYVATMAGLSVGALAVYTPEEGINLNMLKDDIDHLRESFANDKGMNRAGKLILRNEKASKTYTTEIIASIIREEANGRFESRTAIPGHVQQGGTPSPMDRVRAVRLAVKCIQFLESNKKAPKEVFKDPSTSAVIGVRGASVVFTSIEDLRGAETDWDSRRPRKAFWLDYRGLVNTLGGRAGTPTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKA3</accession>
    <name>D5GKA3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00009443001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430337">
        <property type="protein sequence ID" value="CAZ84946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840755.1">
        <property type="nucleotide sequence ID" value="XM_002840709.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKA3"/>
    <dbReference type="GeneID" id="9184875"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009443001"/>
    <dbReference type="HOGENOM" id="CLU_1662085_0_0_1"/>
    <dbReference type="InParanoid" id="D5GKA3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="159" mass="18495" checksum="34546AAEB5C90620" modified="2010-06-15" version="1">MFRKIFNYAKLAGPSPPHHTHTACDRVKLCQTKANPTEAFLRASPLLHPFINHHHFIDLPLVTTVPTKLLEYLHTARPHHQYHYRSPCHLHLPHHPIKASINNGQYWNPNKAPQRGPGPRLHSRTNLWPNIPRQTFRRHFPPSSYPPPPPRSPRLTHVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKE3</accession>
    <name>D5GKE3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00009500001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430338">
        <property type="protein sequence ID" value="CAZ84986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840795.1">
        <property type="nucleotide sequence ID" value="XM_002840749.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKE3"/>
    <dbReference type="GeneID" id="9182006"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009500001"/>
    <dbReference type="HOGENOM" id="CLU_2499512_0_0_1"/>
    <dbReference type="InParanoid" id="D5GKE3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="86" mass="9915" checksum="EE531AB0B17A607F" modified="2010-06-15" version="1">MSSGMSCRTTHASRPRKRIQGLGLYQEKVLDCLREVVVSVITETHSSSPSRVLLEMKVIRPEAAQSQERKGAKERPEEEYRKWMKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKG1</accession>
    <name>D5GKG1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00009528001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430338">
        <property type="protein sequence ID" value="CAZ85004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840813.1">
        <property type="nucleotide sequence ID" value="XM_002840767.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKG1"/>
    <dbReference type="STRING" id="656061.D5GKG1"/>
    <dbReference type="GeneID" id="9182024"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009528001"/>
    <dbReference type="eggNOG" id="ENOG502SGAD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005461_1_0_1"/>
    <dbReference type="InParanoid" id="D5GKG1"/>
    <dbReference type="OMA" id="GVAHARY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23242">
        <property type="entry name" value="TRANSCRIPTION FACTOR HOXA13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23242:SF9">
        <property type="entry name" value="TRANSCRIPTION FACTOR HOXA13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="300"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="396"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="985"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="306"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="985"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="997"/>
            <end position="1006"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1234" mass="130694" checksum="930CCC628ADC4D24" modified="2010-06-15" version="1">MGVAGAKRNVRALRPTPRTTGGGSTLFNVVARILAWYTLYTLLFRCPSVPTHGSPTICHTSHAISNALRPHLEPYYDTYVSPYVTEYGPYVRHANEHYIIPVHKSASSSYEKFAAPHVARGQEYASMGYNRSVKPRVDEVFKKARVYYDGYLSPHVATANEFYATAQPYLIKVRKTAGWFYRELLVPAFEQSLPYVITAYEQLRHIIVTIIAPLVKANGEKAVGWGINVWSEVVGPQVGMIGKRLGGTGSGVPVASIPASMFSSFSSTDTVKATASAASLSSIISSVSAEAASAKASASAQAAESNESKTSENEAQKPTKEEIREIVKADLENWKAKFGAATEKASIDLNAQIDEITTGAKNRQEIHVQREIESLEELIEREFSSLKKVAQELAKPIDPGNGKEHQQASEETFAAKSKKSGINIRDKAQSIRAQSQQFLSEVYADVVKAADNHLEALDSVLDLAMQELGMKWAWMDYVTHKDWKKYHELKKDFDNLRRNVVAAAQKNRKLIETTRWTERNWEGKATEIAKEAADELKRLKTVSKRKIQLSDPSDDFSESTVPVAAHKAAQAIVGEAGGASSAAEKLMGQASEEVVGPEHSTTQSVASVIIEKAKSAVNLASEKAATAIPEAVLGEDESTTVTPSISSVASEIKETIKPKVWGGVYAGFVADPRVEYNVDGDEEGTGEKVRNAVDAASSRLADVSRAVSAAIAGAATTLSPGEKHASVASEKYESAVSAASSALYGTPQPGGESLGSIATDKYSAAVAAASSVIYGTPTPGAKAFAIEAKSKYAAALAAAEESYSSLLASSASAQSQYDYAVKQAQITYQSVIDAASTKVHGTPQPTGESVVSAARSKYQEALSEAQKTYDRWYNAASTTVGTPIPPYQSVASAISKLAGNAASAASEKIYGTPQPITESAASVVGEYVAPATTNAGQKYESMKAFISELVHGKEPTYTESVMSRFSSLFHATPAPLLSRAGSSYSSATTHLSGSHDPTTSTMGSTMTPPPSLESIISYANAQVQSAISVASEQVYGPTYTQGSVESMTSLVSEGSSSARSRASSAIHGRETIAAFESANARISAAIESAKSAISEAVYGTEKGTVEQATSTISKVVSTVTGAAAAGVGDAQAKASEAAYVSEKGFVESAQLRVEVAVESARRKLNEVLYGYATQATEAAGSAYSQATQAASSVASSVSSVIIPPEAGAREKVSSSVGSVTEKVEEAYETVREEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKJ8</accession>
    <name>D5GKJ8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00009596001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M20A family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430339">
        <property type="protein sequence ID" value="CAZ85041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840850.1">
        <property type="nucleotide sequence ID" value="XM_002840804.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKJ8"/>
    <dbReference type="STRING" id="656061.D5GKJ8"/>
    <dbReference type="GeneID" id="9184702"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009596001"/>
    <dbReference type="eggNOG" id="KOG2275">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021802_3_0_1"/>
    <dbReference type="InParanoid" id="D5GKJ8"/>
    <dbReference type="OMA" id="AWESVIF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05652">
        <property type="entry name" value="M20_ArgE_DapE-like_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050072">
        <property type="entry name" value="Peptidase_M20A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43808">
        <property type="entry name" value="ACETYLORNITHINE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43808:SF8">
        <property type="entry name" value="PEPTIDASE M20 DIMERISATION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="6">
        <location>
            <begin position="171"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ85041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="351" mass="37894" checksum="F1E8DBE47C26A2DB" modified="2010-06-15" version="1">MTSDPKSLTDTSAALIELHKNLCEIPSVAPNELEVGDWLATYLESKSFTVEKQEVARNRNNIFAYLGNKRQTKTLVTSHIDVVPPYINYTYHSNGTITGRGTNDAKGCVAAQIKAVEILLAEGLIEEGDVAMLFVVAEETDGAGMIKANDLGLAWESVIFGEPTELKLSLGHKGVLRFIIEAFGKAVHSGYPQLGTNANEQLVGALYILSKMQLPGSDLLGDSTLNYGVMGGGTAPNIISSYANASVAIRLAADPLLVRALVNETVTGLPITLTWFERFYGPQYLDCDVEGFEVEAQAYGTDIPNLKGSHKRYLYGPGSIFTAHSNAEFVTVTDLVESVEGYKALIVHTLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>D5GL10</accession>
    <name>D5GL10_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00009894001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430343">
        <property type="protein sequence ID" value="CAZ85203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841012.1">
        <property type="nucleotide sequence ID" value="XM_002840966.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GL10"/>
    <dbReference type="STRING" id="656061.D5GL10"/>
    <dbReference type="GeneID" id="9185796"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009894001"/>
    <dbReference type="eggNOG" id="KOG0159">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022195_1_0_1"/>
    <dbReference type="InParanoid" id="D5GL10"/>
    <dbReference type="OMA" id="YTTIDHE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11041">
        <property type="entry name" value="CYP503A1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46206">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0349">Heme</keyword>
    <keyword evidence="5 6 7" id="KW-0408">Iron</keyword>
    <keyword evidence="3 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="6">
        <location>
            <position position="333"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR602403-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ85203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="396" mass="44651" checksum="7B22163ADDB068F2" modified="2010-06-15" version="1">MLINLTNREHARRPAGRDTSRLGRRASGISYQWLVLSSGPILVSQWVSVDFFSTALRIIARVSARSFIGLPVCRNEDWLNLAIAFTEDLIATASNLGTISKLFRPFYAYMWNSAKTIKSHKRRAQTLLAPTIQKRFEEQRLAEENGAVYQGPNDLLQWLIDRVEPHHKTVDALSELQLLVGLASVFATARTLVTMIYDLAEHRDCIQPMREEMETVISENGGELDRGVLRKMRKTDSFLRESMRMGTFLLSFNRMAMKNVTLSDGTYLPKGTLVAAPALMFSSDPDFVEDPETFDGFRWYKKTLEAKAGALMGNGSATTSSNNLAFGHGKHACPGRYFAVEEIKTILTFILLQYDIKYPEGQSRPPNIQIGELASPNRTQKIMFKKLPGPKKFSFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>D5GL60</accession>
    <name>D5GL60_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00010021001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430346">
        <property type="protein sequence ID" value="CAZ85253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841062.1">
        <property type="nucleotide sequence ID" value="XM_002841016.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GL60"/>
    <dbReference type="GeneID" id="9182047"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010021001"/>
    <dbReference type="HOGENOM" id="CLU_3351402_0_0_1"/>
    <dbReference type="InParanoid" id="D5GL60"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046784">
        <property type="entry name" value="Eap1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20566">
        <property type="entry name" value="Eap1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ85253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="37" mass="4306" checksum="EB082EF26944C71D" modified="2010-06-15" version="1">MESPVLRRYEIEELLLLRDSPLVQKPAGLPPIEEWMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLJ2</accession>
    <name>D5GLJ2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Histone acetyltransferase type B catalytic subunit</fullName>
            <ecNumber evidence="2 7">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00010233001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalytic component of the histone acetylase B (HAT-B) complex. Has intrinsic substrate specificity that modifies lysine in recognition sequence GXGKXG. Involved in DNA double-strand break repair.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the HAT-B complex composed of at least HAT1 and HAT2. The HAT-B complex binds to histone H4 tail.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the HAT1 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="2 7"/>
    <dbReference type="EMBL" id="FN430349">
        <property type="protein sequence ID" value="CAZ85385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841194.1">
        <property type="nucleotide sequence ID" value="XM_002841148.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLJ2"/>
    <dbReference type="FunCoup" id="D5GLJ2">
        <property type="interactions" value="1084"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GLJ2"/>
    <dbReference type="GeneID" id="9186129"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010233001"/>
    <dbReference type="eggNOG" id="KOG2696">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036024_2_1_1"/>
    <dbReference type="InParanoid" id="D5GLJ2"/>
    <dbReference type="OMA" id="WTCDAND"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.360.10">
        <property type="entry name" value="Histone acetyl transferase 1 (HAT1), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019467">
        <property type="entry name" value="Hat1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037113">
        <property type="entry name" value="Hat1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017380">
        <property type="entry name" value="Hist_AcTrfase_B-typ_cat-su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12046">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE TYPE B CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10394">
        <property type="entry name" value="Hat1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038084">
        <property type="entry name" value="HAT-B_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Histone acetyl transferase HAT1 N-terminal" evidence="13">
        <location>
            <begin position="5"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="12">
        <location>
            <begin position="169"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with histone H4 N-terminus" evidence="9">
        <location>
            <begin position="40"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with histone H4 N-terminus" evidence="9">
        <location>
            <begin position="199"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="384"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="8">
        <location>
            <position position="254"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="219"/>
            <end position="221"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with histone H4 N-terminus" evidence="10">
        <location>
            <position position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR038084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR038084-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR038084-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR038084-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF10394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ85385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="412" mass="47398" checksum="688F9BC0A12153D0" modified="2010-06-15" version="1">MEEEWSADANEAIELSLISTTSGKPIATFHPQFTYGIFGDSENIFGYKDPNLQISFAANDMKCCLDISYEDKMEPQGSVQAEDLEAIITDFLPEDCEKDLKEFQTKIAQRDDTKWSPPGDKISEYTLKGRTYEIWRSTIDDAYCKSILKNIQILITLYIEGGTAIDLEDEEWTNKRWEVFFLYERSAANHTFIGYCTCYRYYLYNPKSPDTSRIRISQFLILPPFQHQGHGKNLYNSLITHFLTITSIQEITVEDPSEAFQNLRDIQDLHRLTPALTRSNLTPQSFSNKSFPGADIRTRAKLPVRQFARVCEMFMLQGIEKGDEKSMKVFRLLVKARIYKQNKDVLAQLDRLERIDRLHDTYLHVEDEYKGLLIAAGAAPVEEEEVEEIETEKKRSANGDGGRASKKVRVAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLY8</accession>
    <name>D5GLY8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00010323001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430351">
        <property type="protein sequence ID" value="CAZ85450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841259.1">
        <property type="nucleotide sequence ID" value="XM_002841213.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLY8"/>
    <dbReference type="GeneID" id="9182753"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010323001"/>
    <dbReference type="HOGENOM" id="CLU_1428962_0_0_1"/>
    <dbReference type="InParanoid" id="D5GLY8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="190" mass="21484" checksum="A6CD98560560A5C6" modified="2010-06-15" version="1">METPVDTGTGLDENENNLTCPSPAPGWYELLIFKRLPQHHHSQQPHPPPPPPHSYRSINNNTPRWTHLQVTTLPISNHAYSRDFKNLDTPKGLLALKSLPAEALAQLERVLREKNYLLRRAGWAWVVDWVDVEKEVGSNGGYGFAGGGGEFCLRVRRVRVHDMGDEWEEDAGRWADVRGIEERGSAMLCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5GM49</accession>
    <name>D5GM49_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00010525001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Component of the 19S cap proteasome complex which acts as a regulatory subunit of the 26S proteasome, involved in the ATP-dependent degradation of ubiquitinated proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">The 26S proteasome is composed of a core protease, known as the 20S proteasome, capped at one or both ends by the 19S regulatory complex (RC). The RC is composed of at least 18 different subunits in two subcomplexes, the base and the lid, which form the portions proximal and distal to the 20S proteolytic core, respectively. Component of the lid subcomplex of the 19S RC.</text>
    </comment>
    <dbReference type="EMBL" id="FN430352">
        <property type="protein sequence ID" value="CAZ85592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841401.1">
        <property type="nucleotide sequence ID" value="XM_002841355.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GM49"/>
    <dbReference type="FunCoup" id="D5GM49">
        <property type="interactions" value="1060"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GM49"/>
    <dbReference type="GeneID" id="9187376"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010525001"/>
    <dbReference type="eggNOG" id="KOG0687">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031814_1_1_1"/>
    <dbReference type="InParanoid" id="D5GM49"/>
    <dbReference type="OMA" id="RLHCKVD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.570:FF:000005">
        <property type="entry name" value="26S proteasome regulatory subunit N7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019585">
        <property type="entry name" value="Rpn7/CSN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045135">
        <property type="entry name" value="Rpn7_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049549">
        <property type="entry name" value="RPN7_PSMD6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14145:SF1">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14145">
        <property type="entry name" value="26S PROTESOME SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10602">
        <property type="entry name" value="RPN7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21154">
        <property type="entry name" value="RPN7_PSMD6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0647">Proteasome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="4">
        <location>
            <begin position="196"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00093435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00093502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ85592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="399" mass="45345" checksum="4CF65A417A3DDC46" modified="2010-06-15" version="1">MGSDPQYASYPNLLLSQHIFTLRTPSLSAHYPTANKALTASIKEASQAPLYRYLAHPTDGILSSKIVWDEKYYEELKKSNDEELEVLDTEIKESDEKAGESEVVEAMGKKAEFFARVVDKERALAAYEELYIKTPTLGAKIDIVLAIIRMEMFYDDKVGVKKSVERARRLIDTGGDWDRRNRLKSYTGLHLLSCRQYAEAAPLLLDSLSTFTSIEICSYSTLVLYAVLAGTISLNRVDFKAKVIDSAEVLADTEGYSSLESLVNSLYLCEYKGFFVALADVEERFLSRDRILAEHKAWYVREMRRRAYAQLLESYRVVGLESMANAFGVSVEWLDRDLSKFIPSKKLNCTIDRVNGVIETNRPDDKNRQYQDVVKQGDQLLTKLQKFGQAVRLRGSERA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-07-27" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5GM60</accession>
    <name>DAPB_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName>Probable dipeptidyl-aminopeptidase B</fullName>
            <shortName>DPAP B</shortName>
            <ecNumber>3.4.14.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">DAPB</name>
        <name type="ORF">GSTUM_00010539001</name>
    </gene>
    <organism>
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Type IV dipeptidyl-peptidase which removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>Release of an N-terminal dipeptide, Xaa-Yaa-|-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline.</text>
            <dbReference type="EC" id="3.4.14.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <text evidence="1">Lysosome-like vacuoles.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase S9B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.14.5"/>
    <dbReference type="EMBL" id="FN430352">
        <property type="protein sequence ID" value="CAZ85603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841412.1">
        <property type="nucleotide sequence ID" value="XM_002841366.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GM60"/>
    <dbReference type="SMR" id="D5GM60"/>
    <dbReference type="FunCoup" id="D5GM60">
        <property type="interactions" value="300"/>
    </dbReference>
    <dbReference type="ESTHER" id="tubmm-dapb">
        <property type="family name" value="DPP4N_Peptidase_S9"/>
    </dbReference>
    <dbReference type="MEROPS" id="S09.006"/>
    <dbReference type="GlyCosmos" id="D5GM60">
        <property type="glycosylation" value="3 sites, No reported glycans"/>
    </dbReference>
    <dbReference type="EnsemblFungi" id="CAZ85603">
        <property type="protein sequence ID" value="CAZ85603"/>
        <property type="gene ID" value="GSTUM_00010539001"/>
    </dbReference>
    <dbReference type="GeneID" id="9187387"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010539001"/>
    <dbReference type="eggNOG" id="KOG2100">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006105_0_1_1"/>
    <dbReference type="InParanoid" id="D5GM60"/>
    <dbReference type="OMA" id="MRTPQEN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000003">
        <property type="entry name" value="Dipeptidyl peptidase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.140.10.30">
        <property type="entry name" value="Dipeptidylpeptidase IV, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002469">
        <property type="entry name" value="Peptidase_S9B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050278">
        <property type="entry name" value="Serine_Prot_S9B/DPPIV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731:SF200">
        <property type="entry name" value="DIPEPTIDYL PEPTIDASE 10, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731">
        <property type="entry name" value="PROTEASE FAMILY S9B,C DIPEPTIDYL-PEPTIDASE IV-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00930">
        <property type="entry name" value="DPPIV_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0031">Aminopeptidase</keyword>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0720">Serine protease</keyword>
    <keyword id="KW-0735">Signal-anchor</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0926">Vacuole</keyword>
    <feature type="chain" id="PRO_0000412166" description="Probable dipeptidyl-aminopeptidase B">
        <location>
            <begin position="1"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="2">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Signal-anchor for type II membrane protein" evidence="2">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="topological domain" description="Vacuolar" evidence="2">
        <location>
            <begin position="110"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="22"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="746"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="823"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="856"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="2">
        <location>
            <position position="185"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="2">
        <location>
            <position position="341"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="2">
        <location>
            <position position="800"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="907" mass="102621" checksum="137143AE5B685B7F" modified="2010-06-15" version="1">MYDQVPYRDTDEATPQIKNDASDSNRSSIDTTSTTSLILERLHREDPDDSPGEYESSGPSQRGKPDEDDDLEIGRAARLKPMERKVRRAMYLLAFLMIGGWFLALAVYVSREHFGTPDTAHDPSATATRKAGKKITLNQVMRGAWRSKTHGIQWINGPHGDQDGLLLTQNSFGDGNFLEVQDVKNDSNTIVLIKDGALQGSGQPVSAIKGWPSSDLKKVLVASDHEKRWRHSYNARYWIYDVEKATTEPLVPSEPEARLSLATWSPKGDAIAFVKDNNVFIRQLGLDLTSEYYSVTQVTKDGGPDLFYGIPDWVYEEEVFSGNSALWWSQDGEFLAFLRTNETEVPEYPIQYFVSRPSGNNPPNGLENYPELEFIKYPKAGAPNPVVHLRFYDLKKKEDFAVTVENDFPDDDRLITEVVWSDGKYLLVRETNRESDVLRMVLIDVSARSGKVVREVDISAIDGGWFEVSKNTRYIPADPASGRPYEGYIDTVIHEGYDHLGYFTPLDNKDPILLTKGQWEVVDAPSAVDLKNGIVYFVATEKSPIERHVYSVKLDGSNFRPVTSTSEDGRYDVSFSKLSGYALLTYEGPGIPWQKVVGTPSGDQSFVKDIEKNQGLATLAAKHELPTFHYSTVNIDGFDLHVVERRPPHFNKKRKYPVLFQVYGGPGSQQVSKSFSIDFQAYIAAGLEYIVVTVDGRGTGFIGRKARVAVRGNLGYWEAHDQIETAKIWGKKGYVDKKRIAIWGWSYGGFMTLKTLEQDAGRTFSYGMAVAPVTDWRFYDSIYTERYMHTPQHNQEGYRNATISDTQALSKSVRFLLMHGVADDNVHMQNSLALLDKLDLASVENYDVHVFPDSDHSIYFHNANRMVYDRLEQWLIRAFNGEFLKLDGLKPIREISEPLRKRNRELV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMU5</accession>
    <name>D5GMU5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00010936001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430359">
        <property type="protein sequence ID" value="CAZ85838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841647.1">
        <property type="nucleotide sequence ID" value="XM_002841601.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMU5"/>
    <dbReference type="STRING" id="656061.D5GMU5"/>
    <dbReference type="GeneID" id="9185174"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010936001"/>
    <dbReference type="eggNOG" id="ENOG502SIT3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004083_7_2_1"/>
    <dbReference type="InParanoid" id="D5GMU5"/>
    <dbReference type="OMA" id="ISRIMMA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050613">
        <property type="entry name" value="Sec_Metabolite_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001:SF77">
        <property type="entry name" value="TRANSCRIPTION FACTOR, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G12940)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001">
        <property type="entry name" value="UNCHARACTERIZED TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00755">
        <property type="entry name" value="AFLATOXINBRP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="5">
        <location>
            <begin position="11"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="84"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="156"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ85838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="663" mass="74042" checksum="6B8E2C9BD88FBEE8" modified="2010-06-15" version="1">MNDDSKNPPRSCTSCRARKIRCDKVKPSCHACLRNRLSCEYPTATRRAAPRPRRDAVLMQRLVKLEAIIGELSGVGGGSAVGELGGARSESDTRGRSLEAWGSQGGSGDMTRESGEVEAELGKLFVSEGKSRYIGPKFWASLIEEVADLKSLLDESPPATVASPTSPDATSSVPSSPLFGSATENLSMFHPTPTESVQLYKRYLVAVDPVARLLHKPTFEPKLARLQENGPESEDLHADRGFEALVFSIYYAAVNSLRPDKVLQMFGVDKPSLLNRYRVAVEHALANAGFLQSEELTTLQALVLFITCSRAVDDTRTPWVLVGVAIRIAQSMGLHRDGSIFRINPLETEIRRRLWYEICLLDLRTAEAHGSDPGISTQSYDTRLPLNIEDADILPNSAYPITPRKGFTEMTFCLVLYEVLDCFRVVVRSTPGAVKVKGVEQPTLEEKEKMLDELALRLAAKYQVELDLTIPIQKITDMVIRLIVGKALLSLYHPLRHFKDGSLLSQGKKDKLFRTAVEALECSTSLVRDPDVVRYEQWNWLSQKYNHAQAASLVLSELVVRPPFGTDMVERAWVAVDLLLGTEPWSRENPIMAERVWKPLGKLRDKALARRRRALQETGMAQRQKPSASLSIGESDIWEGIVVEDVPIDWGEWDEMFQYIDEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMX3</accession>
    <name>D5GMX3_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 16">Phosphatidylinositol transfer protein SFH5</fullName>
            <shortName evidence="16">PITP SFH5</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">GSTUM_00010980001</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Non-classical phosphatidylinositol (PtdIns) transfer protein (PITP), which exhibits PtdIns-binding/transfer activity in the absence of detectable PtdCho-binding/transfer activity. Regulates PtdIns(4,5)P2 homeostasis at the plasma membrane. Heme-binding protein that may play a role in organic oxidant-induced stress responses.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)(out)</text>
            <dbReference type="Rhea" id="RHEA:38691"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:38692"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 16">Endoplasmic reticulum membrane</location>
            <topology evidence="2 16">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Microsome membrane</location>
            <topology evidence="16">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the SFH5 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430360">
        <property type="protein sequence ID" value="CAZ85866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841675.1">
        <property type="nucleotide sequence ID" value="XM_002841629.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMX3"/>
    <dbReference type="FunCoup" id="D5GMX3">
        <property type="interactions" value="37"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GMX3"/>
    <dbReference type="GeneID" id="9183434"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010980001"/>
    <dbReference type="eggNOG" id="KOG1471">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045138_2_0_1"/>
    <dbReference type="InParanoid" id="D5GMX3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008526">
        <property type="term" value="F:phosphatidylinositol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017157">
        <property type="term" value="P:regulation of exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042938">
        <property type="entry name" value="Sfh5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47669">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL TRANSFER PROTEIN SFH5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47669:SF1">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL TRANSFER PROTEIN SFH5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03765">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 16" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 16" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0349">Heme</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="12 16" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 16" id="KW-0492">Microsome</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 16" id="KW-0813">Transport</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="18">
        <location>
            <begin position="132"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="300"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="306"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00024180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU367059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAZ85866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="428" mass="47044" checksum="AC0063B0B49D7972" modified="2010-06-15" version="1">MSNETTTPAALADLHTLIVPLLAESATPTKMWGVTLSTDKDDFHTKLILQKFLRGNKNNVSAAEKQFVETIKWRRGYFDADGKVIGTWDQTKFADLAWITKEKIQSSDQEVVVTWNIYGAVKNFKETFGDVDEFIRWRVTLMERTIDLLELGSVKVPIPENGPDPYKAFQIHDYLQVSILRNHPVIKAASEKAIDLFQNYYPECLDKKFFVNVPLLMGWMYNAMKMVINKDTFKKLYMLRHGASLASELNSETVPEEYGGKGVKLSIKGMTIGADGQLVRVELKPVESGEVDAVAITEMPKGELPQVKEETEERKELAKSKGSEEPNVAEEPKEKKEPETANIDQAAGTADVDQAAGTTNVGQAAGTAMIDQAAGIADTIESAAPAIPTAPISPAAELPVDNSSTLVELETHIQPPALAKNGGENPQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNP4</accession>
    <name>D5GNP4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peroxisomal targeting signal receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Peroxin-5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">GSTUM_00011398001</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peroxisomal targeting signal receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430366">
        <property type="protein sequence ID" value="CAZ86137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841946.1">
        <property type="nucleotide sequence ID" value="XM_002841900.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNP4"/>
    <dbReference type="FunCoup" id="D5GNP4">
        <property type="interactions" value="86"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GNP4"/>
    <dbReference type="GeneID" id="9186213"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011398001"/>
    <dbReference type="eggNOG" id="KOG1125">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013516_3_0_1"/>
    <dbReference type="InParanoid" id="D5GNP4"/>
    <dbReference type="OMA" id="WEEQFKQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005052">
        <property type="term" value="F:peroxisome matrix targeting signal-1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016560">
        <property type="term" value="P:protein import into peroxisome matrix, docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000218">
        <property type="entry name" value="Peroxisomal targeting signal receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024111">
        <property type="entry name" value="PEX5/PEX5L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10130:SF0">
        <property type="entry name" value="GH08708P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10130">
        <property type="entry name" value="PEROXISOMAL TARGETING SIGNAL 1 RECEPTOR PEX5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="13" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0882">Thioester bond</keyword>
    <keyword evidence="9 15" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <feature type="repeat" description="TPR" evidence="15">
        <location>
            <begin position="343"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="15">
        <location>
            <begin position="451"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="15">
        <location>
            <begin position="490"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="22"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="23"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAZ86137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="618" mass="68039" checksum="213CCFA527ECC43E" modified="2010-06-15" version="1">MSFLGGAECSTGRNPLAQFTKHVSKDKSLERDRLGSLPVGPELGGLRTAGGEMAGADKQMMDEFYSQQSGASPFAFESMRRELSDLQHGPAPMAGQPWASEFGTQQAMNPQEQAMMEAAFVARNKSSEFSTAEFARFQNSPSSGDIQMALPAPTQAASTPYYQQFRGYNNFNQGLPFQVHQPMIQQLQMQNQQQDPKGKGKQVVELSDENWESQFKEMEAMNKTLDADDQANKDIEAELNSQPETMYGDFQSVWEGIQAETGGAALNGVDWQNEFDTSNPWSAEASATNSMPNLGEYLFELDNPYLMHEDPFSEGKRLMESGGNLSLAALAFEAAVQKDKNHVEAWTELGGCQAANEKETPAIRALEAALKLDENNLGALMGLAVSYTNEGYDSTAYSTLERWLATKYPQVTEQAPPAPISTLDRMAIHERVTNLFIQAAQLSPEGTAMDSDVQVGLGVLFYGAEEYDKAVDCFSAALTSTSTGSSSPEHLLWNRLGATLANSGRSEEAINAYERALTINPNFVRARYNLGVSCINIGCYEQAAEHLLGALAMHKVAEERARQDFSEVGLDPARIVQNQSTNLFDTLRRVFSQMGRRDLAELVTNGMDIDVFRKEFEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNQ7</accession>
    <name>D5GNQ7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00011430001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="3">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SMP-30/CGR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430367">
        <property type="protein sequence ID" value="CAZ86154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841963.1">
        <property type="nucleotide sequence ID" value="XM_002841917.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNQ7"/>
    <dbReference type="FunCoup" id="D5GNQ7">
        <property type="interactions" value="31"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GNQ7"/>
    <dbReference type="GeneID" id="9182789"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011430001"/>
    <dbReference type="eggNOG" id="KOG4499">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036110_3_0_1"/>
    <dbReference type="InParanoid" id="D5GNQ7"/>
    <dbReference type="OMA" id="WAGTMRY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004341">
        <property type="term" value="F:gluconolactonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013658">
        <property type="entry name" value="SGL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005511">
        <property type="entry name" value="SMP-30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10907">
        <property type="entry name" value="REGUCALCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10907:SF47">
        <property type="entry name" value="REGUCALCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08450">
        <property type="entry name" value="SGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01790">
        <property type="entry name" value="SMP30FAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="SMP-30/Gluconolactonase/LRE-like region" evidence="5">
        <location>
            <begin position="23"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="332"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="333"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="343"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="2">
        <location>
            <position position="220"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR605511-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR605511-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ86154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="377" mass="40803" checksum="7E4DCA888942E7BF" modified="2010-06-15" version="1">MPADFKVIAKTGDPILHREDFWLPESPVHDEDTGTTWFTDIPRSVIYGFNVKEGQKSLRRIEVGDLVGCLALIDGDKEHLAVGAKRGFGKVNIETGKLEYINTLFPGDKDKQDLMRINDGAVDVRGRFWAGSMRSFGLGPPQNEGTLFRSDPTTHLNHVKYPVAIPNGIGFSPDNSTLYLVETRLKTIFAYRFEPETGGISHEQEFIKFDEDKHGPGVPDGLAISSDGDVWVAMRDGHKVLRIDPRTKDVKGIIEVPTSKQVACPAFVGEGMVITTGKLVHLDPDVAKTSAHAGDVFYVPLPGITGREVYKARFDAVPEDPGTTSGKLLSALKGKEREKEDAGAPTGAAAPPAVEIPTASATTAVTTATVTRVTGEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPA5</accession>
    <name>D5GPA5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00011776001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430373">
        <property type="protein sequence ID" value="CAZ86370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842179.1">
        <property type="nucleotide sequence ID" value="XM_002842133.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPA5"/>
    <dbReference type="FunCoup" id="D5GPA5">
        <property type="interactions" value="38"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GPA5"/>
    <dbReference type="GeneID" id="9186610"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011776001"/>
    <dbReference type="eggNOG" id="KOG4580">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_141247_1_0_1"/>
    <dbReference type="InParanoid" id="D5GPA5"/>
    <dbReference type="OMA" id="MAIMIYA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033254">
        <property type="term" value="C:vacuolar transporter chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003807">
        <property type="entry name" value="DUF202"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051572">
        <property type="entry name" value="VTC_Complex_Subunit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46140">
        <property type="entry name" value="VACUOLAR TRANSPORTER CHAPERONE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46140:SF1">
        <property type="entry name" value="VACUOLAR TRANSPORTER CHAPERONE COMPLEX SUBUNIT 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02656">
        <property type="entry name" value="DUF202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="60"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="DUF202" evidence="6">
        <location>
            <begin position="24"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ86370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="122" mass="13426" checksum="0D8D59D96015D0A7" modified="2010-06-15" version="1">MSSQPLLQSAPGKRIALPTRVEPKVFFANERTFLSWLNFTVILGGLAVGLLNFGDKVGRISASLFTFVAMGTMLYALVTFHWRAAAIRKRGQGSFDDRFGPTVLALTLFAAVIVNFVLRVFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPC8</accession>
    <name>D5GPC8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00011673001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430373">
        <property type="protein sequence ID" value="CAZ86290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842099.1">
        <property type="nucleotide sequence ID" value="XM_002842053.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPC8"/>
    <dbReference type="STRING" id="656061.D5GPC8"/>
    <dbReference type="GeneID" id="9186530"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011673001"/>
    <dbReference type="eggNOG" id="ENOG502S7WN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030520_0_0_1"/>
    <dbReference type="InParanoid" id="D5GPC8"/>
    <dbReference type="OMA" id="DERTMTM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="212"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="120"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="306"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="341"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ86290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="397" mass="42881" checksum="11503D625F9FB1F0" modified="2010-06-15" version="1">MVDYRAPAYQFPLKKPMGPDAPPPLPTKNSLRISPNHSSQSSYDLQSATSPSSHVRCRTSSSGTLPMQGTQWMQTYTQNYNHNYNHNYPQNPHMNRRPSVASNSRGGAMDPTVPITIRRSGSTHTTNSASTSAQSAYVATLRRQKATVWCDRSQREDPRLLADQRYAKLRAAMEVVGSSNSAFLSAGHNHHGIGSAAGSAASSISGGRTGKLGKTWVSSSSPGIGGGSLPSGVPTRLSATEVLGDDDEDDLYPRGAGIRKRSGSGKSSLNSNHRRINRSSSMMSDTNRNYTMYGGAPGGLQHRNSRGSTPSYSSRSSITEEGTPSTTTKTKHKSEKLPESPSGSTSPRAYYTPTLQSSTILRRDNTLRRRGSLDEQEVVRTMTLSGNRLFVANPDVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPM4</accession>
    <name>D5GPM4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00011924001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430376">
        <property type="protein sequence ID" value="CAZ86467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPM4"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011924001"/>
    <dbReference type="HOGENOM" id="CLU_2225122_0_0_1"/>
    <dbReference type="InParanoid" id="D5GPM4"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ86467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="106" mass="11760" checksum="B148E65906CCAAEC" modified="2010-06-15" version="1">MLPAVPSILHCTLQCCPGWLLLSPNPANINNPSRPVARRIASMMSSTFDPSRAPNSTITRQSLIRRKNPPPRLPSPDRTTQSICVLTLALCERAGSRMDRYCIMKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPU0</accession>
    <name>D5GPU0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00012030001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AHA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430378">
        <property type="protein sequence ID" value="CAZ86533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842342.1">
        <property type="nucleotide sequence ID" value="XM_002842296.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPU0"/>
    <dbReference type="FunCoup" id="D5GPU0">
        <property type="interactions" value="1268"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GPU0"/>
    <dbReference type="GeneID" id="9182277"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012030001"/>
    <dbReference type="eggNOG" id="KOG2936">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049046_1_0_1"/>
    <dbReference type="InParanoid" id="D5GPU0"/>
    <dbReference type="OMA" id="GDCEVNQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd08892">
        <property type="entry name" value="SRPBCC_Aha1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.20">
        <property type="entry name" value="Activator of Hsp90 ATPase Aha1, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036338">
        <property type="entry name" value="Aha1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015310">
        <property type="entry name" value="AHSA1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013538">
        <property type="entry name" value="ASHA1/2-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13009">
        <property type="entry name" value="HEAT SHOCK PROTEIN 90 HSP90 CO-CHAPERONE AHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13009:SF22">
        <property type="entry name" value="LD43819P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09229">
        <property type="entry name" value="Aha1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08327">
        <property type="entry name" value="AHSA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01000">
        <property type="entry name" value="Aha1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103111">
        <property type="entry name" value="Activator of Hsp90 ATPase, Aha1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Activator of Hsp90 ATPase AHSA1-like N-terminal" evidence="3">
        <location>
            <begin position="13"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="146"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="180"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ86533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="324" mass="35830" checksum="7CB92CC3A3D050FD" modified="2010-06-15" version="1">MVLHNPNNWHWVNKNAIEWAQEYFSEKLTGLSANKGEVSAKIDKVLSVEGDCDVSQRKGKVITLFDVKVRLEFSGVTAEGEKVTGSITIPEVAHDTEEGEYVFEINVYSDNKEKQAVRDLVRNDIVPQLRQALAGFSGDLIAEHGKDIQHAPGSGPVSGTSTPKPAATSSTSHAASSKTQPSQTETSPIVNTVTLKDDIEFQTSADQLYQTFVDPQRVAAFTRAPPSEFEPREGGKFSLFGRNVEGQFKQLDKDKKIVQTWRLADWPKGHYSTLTLVFDQSTNSTVLRLSWDGVPVGQEDVTKRNFGDYYVRSIKTTFGFGAVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5GQ74</accession>
    <name>D5GQ74_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitochondrial pyruvate carrier</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00012227001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Mediates the uptake of pyruvate into mitochondria.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion inner membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the mitochondrial pyruvate carrier (MPC) (TC 2.A.105) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430382">
        <property type="protein sequence ID" value="CAZ86667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842476.1">
        <property type="nucleotide sequence ID" value="XM_002842430.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GQ74"/>
    <dbReference type="FunCoup" id="D5GQ74">
        <property type="interactions" value="243"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GQ74"/>
    <dbReference type="GeneID" id="9185247"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012227001"/>
    <dbReference type="eggNOG" id="KOG1589">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099502_1_1_1"/>
    <dbReference type="InParanoid" id="D5GQ74"/>
    <dbReference type="OMA" id="ITPRVYS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006850">
        <property type="term" value="P:pyruvate import into mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005336">
        <property type="entry name" value="MPC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14154">
        <property type="entry name" value="UPF0041 BRAIN PROTEIN 44-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03650">
        <property type="entry name" value="MPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ86667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="124" mass="13575" checksum="EE55F9DC5953D1FD" modified="2010-06-15" version="1">MAAQAPAQAAKVLNPAFNPRTVHFWAPILKWGLVIAGASDFYRPVESLSVTQNVALFCTGSIWTRWCLIIHPKNIPLAAVNFFLAGVGSVQLGRIGLHYQSLKKQEKENPVMSEIAKKATNTTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5G405</accession>
    <name>D5G405_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00003893001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetolactate synthase small subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835127.1">
        <property type="nucleotide sequence ID" value="XM_002835081.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G405"/>
    <dbReference type="FunCoup" id="D5G405">
        <property type="interactions" value="289"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G405"/>
    <dbReference type="GeneID" id="9182375"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003893001"/>
    <dbReference type="eggNOG" id="KOG2663">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055003_0_0_1"/>
    <dbReference type="InParanoid" id="D5G405"/>
    <dbReference type="OMA" id="CARSGMM"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00055"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00059"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005948">
        <property type="term" value="C:acetolactate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990610">
        <property type="term" value="F:acetolactate synthase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04878">
        <property type="entry name" value="ACT_AHAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.260:FF:000001">
        <property type="entry name" value="Acetolactate synthase, small subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1150">
        <property type="entry name" value="ACT-like. Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004789">
        <property type="entry name" value="Acetalactate_synth_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027271">
        <property type="entry name" value="Acetolactate_synth/TF_NikR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019455">
        <property type="entry name" value="Acetolactate_synth_ssu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039557">
        <property type="entry name" value="AHAS_ACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054480">
        <property type="entry name" value="AHAS_small-like_ACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053050">
        <property type="entry name" value="ALS_regulatory_subunit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00119">
        <property type="entry name" value="acolac_sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31242">
        <property type="entry name" value="ACETOLACTATE SYNTHASE SMALL SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31242:SF2">
        <property type="entry name" value="ACETOLACTATE SYNTHASE SMALL SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22629">
        <property type="entry name" value="ACT_AHAS_ss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10369">
        <property type="entry name" value="ALS_ss_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACT" evidence="6">
        <location>
            <begin position="85"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ79248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="313" mass="33704" checksum="2737C67CCB65870F" modified="2010-06-15" version="1">MAAVRTTCSSLLGRPAINALSRAIGRVYNSSSSSSSTSAIYYKALHRRVNPLPTADPPAWSASAAVSSILYETPLPSSNPPKRHILNCLVQNEPGVLSRVSGILAARGFNIDSLVVCNTEVPDLSRMTIVLSGQDGVVEQARRQLEDLVPVWAVLDYTAAPLIQRELLLAKVSILGPEYFEELLAHHREITAENVEALGEHGVAPGDGDYHPSKLPISQALRHKHQHLHAVTQLTHQFGGKVSDISENNCIVELSAKPSRIDAFMKLIGPFGILESARTGLMALPRSPLQTRAEEIEVKDAGDVVDASTLPPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4K3</accession>
    <name>D5G4K3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00004193001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429988">
        <property type="protein sequence ID" value="CAZ79446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835289.1">
        <property type="nucleotide sequence ID" value="XM_002835243.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4K3"/>
    <dbReference type="GeneID" id="9188396"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004193001"/>
    <dbReference type="HOGENOM" id="CLU_3360018_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4K3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ79446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="36" mass="3851" checksum="C53AAE2ED9359306" modified="2010-06-15" version="1">MTAKEACTITNAKKVVSEAQGLAYPQAGYLSYQTLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4W3</accession>
    <name>D5G4W3_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">dipeptidyl-peptidase IV</fullName>
            <ecNumber evidence="4">3.4.14.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Dipeptidyl peptidase IV</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">GSTUM_00000119001</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of an N-terminal dipeptide, Xaa-Yaa-|-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline.</text>
            <dbReference type="EC" id="3.4.14.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase S9B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.14.5" evidence="4"/>
    <dbReference type="EMBL" id="FN429992">
        <property type="protein sequence ID" value="CAZ79556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835399.1">
        <property type="nucleotide sequence ID" value="XM_002835353.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4W3"/>
    <dbReference type="ESTHER" id="tubmm-d5g4w3">
        <property type="family name" value="DPP4N_Peptidase_S9"/>
    </dbReference>
    <dbReference type="MEROPS" id="S09.008"/>
    <dbReference type="GeneID" id="9183038"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000119001"/>
    <dbReference type="eggNOG" id="KOG2100">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006105_0_2_1"/>
    <dbReference type="InParanoid" id="D5G4W3"/>
    <dbReference type="OMA" id="NTEYRAR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.140.10.30">
        <property type="entry name" value="Dipeptidylpeptidase IV, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002469">
        <property type="entry name" value="Peptidase_S9B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050278">
        <property type="entry name" value="Serine_Prot_S9B/DPPIV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731:SF162">
        <property type="entry name" value="DIPEPTIDYL PEPTIDASE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731">
        <property type="entry name" value="PROTEASE FAMILY S9B,C DIPEPTIDYL-PEPTIDASE IV-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00930">
        <property type="entry name" value="DPPIV_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="10" id="KW-0720">Serine protease</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Dipeptidylpeptidase IV N-terminal" evidence="14">
        <location>
            <begin position="5"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="13">
        <location>
            <begin position="333"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAZ79556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="497" mass="56483" checksum="60F1528E39968188" modified="2010-06-15" version="1">MGWKIFRDRYTSWFSPDGEYLAFLRLMQVPTYTVPRYMAGKKVATPYPEERRIKFPKVGEKNPTLTFHLLETYTPDNLVITDVAWVAEKHERVIVRTQDRTQDTEKLVLVDVESGNSRVVRERNATDGWLDNNLAITYVPRLSTPSYLGISDYSGWAHIYLFPEVTTIYSIDTKRGLVYYQSTERDSTERHIFSVSLDGKTKKSLVDITKERYYDASFSPGGGYYILSYSGPGLPWEELRSISPDTPVRVINDNASLKNKLSEYALPKISWSTLKHPDGYELNFMEYLPPNFHKGKKYPVIFRIYGGPGSQRTQKVFRQTIDFITYLGLDPELEYIVITVDNRGTGFKGRKFRSLVTGQLGKLEAEDQVWAAREYAKRDYVDAKKIAIWGRSYGAYLTGKVLELDSGVFSLGLMTSPGADWPLYDTMFTERHVKLLATNRAGYNASAIIKTAGFKNVAGGFLIQHGTGDDNAHFQHAVVLVDALMYGGVGPEEMRVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5G580</accession>
    <name>D5G580_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00000347001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429997">
        <property type="protein sequence ID" value="CAZ79673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835516.1">
        <property type="nucleotide sequence ID" value="XM_002835470.1"/>
    </dbReference>
    <dbReference type="GeneID" id="9188092"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000347001"/>
    <dbReference type="HOGENOM" id="CLU_721970_0_0_1"/>
    <dbReference type="InParanoid" id="D5G580"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ79673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="383" mass="43064" checksum="56BDA930A7B6273B" modified="2010-06-15" version="1">MIFLRLQISPIQILPPLKVHSNLTPMPSWXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXESEMPWSPLLSDPVKTFPRLLILPRCLNFVQHCVQYILCYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5G6</accession>
    <name>D5G5G6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00004326001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5G6"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004326001"/>
    <dbReference type="HOGENOM" id="CLU_3430008_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5G6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ79759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="19" mass="2179" checksum="59DCC2C5DAB50BFA" modified="2010-06-15" version="1">MSELPDRVAGARDEWKTAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5V8</accession>
    <name>D5G5V8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Signal peptidase subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00001595001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Essential component of the signal peptidase complex (SPC) which catalyzes the cleavage of N-terminal signal sequences from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum. Essential for the SPC catalytic activity, possibly by stabilizing and positioning the active center of the complex close to the lumenal surface. Essential for viability.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the SPCS3 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430002">
        <property type="protein sequence ID" value="CAZ79901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835744.1">
        <property type="nucleotide sequence ID" value="XM_002835698.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5V8"/>
    <dbReference type="FunCoup" id="D5G5V8">
        <property type="interactions" value="394"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G5V8"/>
    <dbReference type="GeneID" id="9187579"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001595001"/>
    <dbReference type="eggNOG" id="KOG3372">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068714_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5V8"/>
    <dbReference type="OMA" id="WVGALTW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005787">
        <property type="term" value="C:signal peptidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045047">
        <property type="term" value="P:protein targeting to ER"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007653">
        <property type="entry name" value="SPC3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12804">
        <property type="entry name" value="MICROSOMAL SIGNAL PEPTIDASE 23 KD SUBUNIT SPC22/23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12804:SF0">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04573">
        <property type="entry name" value="SPC22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016089">
        <property type="entry name" value="SPC22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR016089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ79901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="186" mass="20597" checksum="3C6002B9872FED6E" modified="2010-06-15" version="1">MHSTLVRAQNVFGFLTTVAFFVAGLVALSSLLYPADVNVDVIGTNNVKIAKGRAHQYYTSKVQEYAVLYFDLEADLSSLFNWNTKQVFAYVSATYPGSKYTDNEIVIWDTILPSRSASKLILKNERAKYSINDITGKFAGRDNVTLSFHWNVQPHVGRLTWGAVGLTKGVKFPGVERPGGKKEAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5Y9</accession>
    <name>D5G5Y9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00001520001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430004">
        <property type="protein sequence ID" value="CAZ79932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835775.1">
        <property type="nucleotide sequence ID" value="XM_002835729.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5Y9"/>
    <dbReference type="GeneID" id="9187611"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001520001"/>
    <dbReference type="HOGENOM" id="CLU_1240927_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5Y9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ79932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="223" mass="24086" checksum="B38211E5169E860C" modified="2010-06-15" version="1">MQVLFTHQSVQDSGVIGENVLDIKFSNDLSTSLTLSIRPFTIGCDIKKIPTSTPALSAPSSPPLPPLLSASASVMGAGVEEPEPVAFHPPPAPATSIATQTSLPTHAVASPPPPASLLDTYKVRQLDRIFTADRFYKYLLSAPSTSAYITTDDGFLSALHGITGAEDCSADLVVEAYDGMNELRRAMMAEVIKVVEEGLERDENERLDEWVKIEVPKILDEFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6J1</accession>
    <name>D5G6J1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein uL13m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00004502001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the universal ribosomal protein uL13 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430009">
        <property type="protein sequence ID" value="CAZ80134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835977.1">
        <property type="nucleotide sequence ID" value="XM_002835931.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6J1"/>
    <dbReference type="FunCoup" id="D5G6J1">
        <property type="interactions" value="677"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G6J1"/>
    <dbReference type="GeneID" id="9181219"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004502001"/>
    <dbReference type="eggNOG" id="KOG3203">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082184_1_1_1"/>
    <dbReference type="InParanoid" id="D5G6J1"/>
    <dbReference type="OMA" id="HKPIYTP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00392">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1180.10:FF:000007">
        <property type="entry name" value="50S ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1180.10">
        <property type="entry name" value="Ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01366">
        <property type="entry name" value="Ribosomal_uL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005822">
        <property type="entry name" value="Ribosomal_uL13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005823">
        <property type="entry name" value="Ribosomal_uL13_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023563">
        <property type="entry name" value="Ribosomal_uL13_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036899">
        <property type="entry name" value="Ribosomal_uL13_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01066">
        <property type="entry name" value="rplM_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL13M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545">
        <property type="entry name" value="RIBOSOMAL PROTEIN L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00572">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52161">
        <property type="entry name" value="Ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00783">
        <property type="entry name" value="RIBOSOMAL_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 8" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="151"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="161"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00068950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ80134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="179" mass="20120" checksum="C7B4A4379F8E3183" modified="2010-06-15" version="1">MSTNIGRTSLAYSRSWHLVDMGHDQRSFGRIATSIATTLMGKHKPTYDPSTDCGDYVVAVGCAYLKTTGDKRMKKKYYSHSTRPGHLQEITMDRMMKKFGGAEVLRRAVSGMLPKNRLREVRLGRLRAFEGFGHPYKENLVRLGGKHYVKPRPAAGYPSKTPVQDTINMEQSKPEALVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6K3</accession>
    <name>D5G6K3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00001802001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430010">
        <property type="protein sequence ID" value="CAZ80146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835989.1">
        <property type="nucleotide sequence ID" value="XM_002835943.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6K3"/>
    <dbReference type="GeneID" id="9187182"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001802001"/>
    <dbReference type="HOGENOM" id="CLU_1504526_0_0_1"/>
    <dbReference type="InParanoid" id="D5G6K3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ80146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="179" mass="18773" checksum="38D2438C9D3A68B7" modified="2010-06-15" version="1">MNGCSACFLEFDIMPLPTASPRIIILSQSSLSLRFNPGGFGGRSFFPTFQWRSLSSKVPSLDELPSSSSGGPSGGIPTFPGGSLFLPFPLFPPAGPGSPSNGARPFPFSSWDGLSGGGISTTLAVGRASPTATCSTLSSNPAIWARISANSASRRWSNSLWTRWPRSAASFWFLASCTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6V3</accession>
    <name>D5G6V3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00002289001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430016">
        <property type="protein sequence ID" value="CAZ80246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="tml:GSTUM_00002289001"/>
    <dbReference type="HOGENOM" id="CLU_3436824_0_0_1"/>
    <dbReference type="InParanoid" id="D5G6V3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ80246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="12" mass="1242" checksum="23F4D9758CB879C8" modified="2010-06-15" version="1">MGDNGFGSTDQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>D5G749</accession>
    <name>D5G749_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="11">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00002343001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the CYC8/SSN6 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430021">
        <property type="protein sequence ID" value="CAZ80342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836151.1">
        <property type="nucleotide sequence ID" value="XM_002836105.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G749"/>
    <dbReference type="STRING" id="656061.D5G749"/>
    <dbReference type="GeneID" id="9184276"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002343001"/>
    <dbReference type="eggNOG" id="KOG1124">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006762_2_1_1"/>
    <dbReference type="InParanoid" id="D5G749"/>
    <dbReference type="OMA" id="LYHQQSN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017053">
        <property type="term" value="C:transcription repressor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000078">
        <property type="entry name" value="Transcriptional corepressor Cyc8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000163">
        <property type="entry name" value="Transcriptional corepressor Cyc8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000282">
        <property type="entry name" value="Transcriptional corepressor Cyc8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051630">
        <property type="entry name" value="Corepressor-Demethylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14017:SF1">
        <property type="entry name" value="LD02225P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14017">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12895">
        <property type="entry name" value="ANAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4 9" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="45"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="113"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="149"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="186"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
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            <begin position="223"/>
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        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="295"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="329"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="410"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="23"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="410"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="451"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="494"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="527"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="543"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="635"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="664"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="696"/>
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        </location>
    </feature>
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        <location>
            <begin position="711"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="743"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="771"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="805"/>
            <end position="818"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ80342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="818" mass="91251" checksum="BCAF0F12997147B8" modified="2010-06-15" version="1">MASVQHTTSPGQPQALHQTNGHPPQSIQQQQSKPTAFQQLTQVNEAVWLQIGSVTELMGDLDRAMAAYENALRQNPYSIPAMNSISCILRTKEQFAKAVEYLQSILNLDATNGDIWGSLGHCYLMMDELQKAYSAYQQALYHLRDPKEPKLWYGIGILYDRYGSLEHAEEAFSQVMRMEPNFEKANEIYFRLGIIYKQQEKYRQSLECFRYIVTDPPRPLTEEDIWFQIGHVHEQQKDYASAKDAYMRVLDSDPNHAKVLQQLGWLHHQQSNNFSSQELAIEYLEKSVSADNSDAQSWYLLGRCYMSQQKYPKAYEAYQQAVYRDGRNPTFWCSIGVLYYQINQYRDALDAYSRAIRLNPYISEVWYDLGTLYESCNNQTNDALDAYQRAAELDPTNQHIKARLSLLRNGQSSGVQHQASAPPPQDVHPQAYQAAGVGGPPGPQWGSQAPQAPPPAPSGPPPTLPQIPQPPNPSSYRPTDVPPQPPQPSRHMAHSPDDLRRPVEDRHTQLRRGLSPSPKFPNHSPHPSYPPPPLQGPPPPQQQPQQQQQPPPNRILNPHHNLHNLPPASNNPGGISLPPYGGRGASPPPDVKPIVDDRLPSPNTYPHHPHHPSSQGGIAGGAPPPASAAMAAEAAARERDEKIHHNKRGREWGEDGGQGMVKKPASEEAKARMDDMHHHRQPSPVRASSPHRPERRRSSSERPKEDYHHGPPAPLHTPNVPPLLSHQQLPPINDGPRMSEPPRIADVHRVSEAPRDDRKEIVEPAARKVELDEDYDNEEMGDADRVPGGNPGVQVKENNSPVGNGTNGISGGGAPGDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7R2</accession>
    <name>D5G7R2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00004687001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430031">
        <property type="protein sequence ID" value="CAZ80555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836364.1">
        <property type="nucleotide sequence ID" value="XM_002836318.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7R2"/>
    <dbReference type="GeneID" id="9181374"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004687001"/>
    <dbReference type="eggNOG" id="ENOG502RYAY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010112_0_0_1"/>
    <dbReference type="InParanoid" id="D5G7R2"/>
    <dbReference type="OMA" id="YEVAPWE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046623">
        <property type="entry name" value="DUF6536"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35395">
        <property type="entry name" value="DUF6536 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35395:SF1">
        <property type="entry name" value="DUF6536 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20163">
        <property type="entry name" value="DUF6536"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="117"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="160"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="229"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="498"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="589"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="645"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="750"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="domain" description="DUF6536" evidence="3">
        <location>
            <begin position="110"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ80555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="854" mass="94814" checksum="C806912A90BF176C" modified="2010-06-15" version="1">MFLGQGRRRHGSNANIELYQKVLTNDKGDVGGSSSWRDDDESKVFSEVRTRGLREDEGSNPDDLGASPSSISLYPSQEPSTTRSKLSTSSSRFSILKGPVVSILDTSISWRTGVKMCASIAATIFLFTLGVTIYFIAAYPLEKSGLGTLYLGDCDEMKKISIWAHVAINFVSSILLAGSHYTQQVLSAPTRGEIDAAHETKDWLDIGIPSLRNLKRVGHLHRMERIRVWVWWTLGFTSVPMHLLFNSAIFSTLSLNNYYVGIVTPDFFNGASYNQTGHVISGLRAKTMGSVIIEPFVEGFNYTFWDPNQPGKRYVSKYIYGGETIRAGNVRIEGSFYNTTNSSDSVAVKIMKEMSSDFWMPEYNFEQMREVAQTMSEESWAWHKMDTKECRAKYSRPLLGDMQNLVLVSSTEPKGNSSLMSMIIYNSLEKKHYWMCQNESSLIDIHNWNKTGYAFPKGCQMGADDGERDIPDGDWVVRGHTITECRGQISRKKCKLQFSVGILTVVIIANLLKAIGMAMAAFRMKTVPLVFMGDAIESFLNEPDITTLRRCLADKTMAESSWELQPAPKPYAPEQWRWWHAPSKRRRSITVGTWVGIISLSAIFIGAGYAKDARNGKETDIKSLVKHGFGAQTTSSVLSSRDGGLIIPIFLANSPQVVLSFLYLTYNGIFTSMFMAKEWMDFAIKRKPLRVSQPIGKQRSTYWLQLPYRYSLPLMALKSITHLCASQGIFLAKFEVFSPLGETFKVVSTVGYSVVGIALLMVSGLIHLIVLFAIGSKKYPLGMPLAGSCSAAIAAACHPPEGDMDASYEPLMWGTVPAKSGSTAEPEVGHCSLSSLPVDQPIIGHLYAGKDKDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7V4</accession>
    <name>D5G7V4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00002669001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS14 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430032">
        <property type="protein sequence ID" value="CAZ80597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836406.1">
        <property type="nucleotide sequence ID" value="XM_002836360.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7V4"/>
    <dbReference type="FunCoup" id="D5G7V4">
        <property type="interactions" value="518"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G7V4"/>
    <dbReference type="GeneID" id="9181416"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002669001"/>
    <dbReference type="eggNOG" id="KOG1741">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139869_2_0_1"/>
    <dbReference type="InParanoid" id="D5G7V4"/>
    <dbReference type="OMA" id="FGLCRNQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1480:FF:000001">
        <property type="entry name" value="30S ribosomal protein S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001209">
        <property type="entry name" value="Ribosomal_uS14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018271">
        <property type="entry name" value="Ribosomal_uS14_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19836">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19836:SF19">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US14M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00253">
        <property type="entry name" value="Ribosomal_S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00527">
        <property type="entry name" value="RIBOSOMAL_S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ80597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="115" mass="13201" checksum="552D514E5EA1E769" modified="2010-06-15" version="1">MFPTLRLSKALNLGSFVNARVLRDHARREAFAAAELTRQALRYIAQNTQLPLLQRTKAQVDLALMDSNTRRMKIKNRCIVSGRGRGILRNFRICRYQFRLMGKAGDLPGVKKANW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8C2</accession>
    <name>D5G8C2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00004734001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430042">
        <property type="protein sequence ID" value="CAZ80765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836574.1">
        <property type="nucleotide sequence ID" value="XM_002836528.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8C2"/>
    <dbReference type="GeneID" id="9188367"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004734001"/>
    <dbReference type="HOGENOM" id="CLU_3279816_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8C2"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ80765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="41" mass="4248" checksum="700221C49A9ACF76" modified="2010-06-15" version="1">MSNHAARMSGAWLGGDARVDRVLELVGGTVVGGGEGKRDKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8G1</accession>
    <name>D5G8G1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transcription elongation factor 1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00002850001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Transcription elongation factor implicated in the maintenance of proper chromatin structure in actively transcribed regions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the ELOF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430043">
        <property type="protein sequence ID" value="CAZ80804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836613.1">
        <property type="nucleotide sequence ID" value="XM_002836567.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8G1"/>
    <dbReference type="STRING" id="656061.D5G8G1"/>
    <dbReference type="GeneID" id="9186036"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002850001"/>
    <dbReference type="eggNOG" id="KOG3214">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1210559_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8G1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.190:FF:000001">
        <property type="entry name" value="Transcription elongation factor 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007808">
        <property type="entry name" value="Elf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038567">
        <property type="entry name" value="T_Elf1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20934">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20934:SF0">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05129">
        <property type="entry name" value="Zn_ribbon_Elf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="178"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ80804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="229" mass="25524" checksum="F37646B46F59B997" modified="2010-06-15" version="1">MEAFVLTMLTRVDMSSAVSKLSGADCCSPPSHRINTTPHDNPPPPTTITTAKDTFLLRYTEHLSDNHRPQGRQQPYYNSKPQFTQVIDMGKRKRSTRKPNVFKKKEPLSTTFMCLFCNNQDTVACVLDKKAGIGSLSCRACGQRFKMNINYLSAPIDVYSEWVDACDEIENPKVKAPRRVCGNSRDVGRGAREGVGGAARNKNPRNHGERDEQADEDSDDNHEGYGGKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8H6</accession>
    <name>D5G8H6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00002877001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430046">
        <property type="protein sequence ID" value="CAZ80823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836632.1">
        <property type="nucleotide sequence ID" value="XM_002836586.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8H6"/>
    <dbReference type="FunCoup" id="D5G8H6">
        <property type="interactions" value="650"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G8H6"/>
    <dbReference type="GeneID" id="9186043"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002877001"/>
    <dbReference type="eggNOG" id="KOG2606">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034963_2_0_1"/>
    <dbReference type="InParanoid" id="D5G8H6"/>
    <dbReference type="OMA" id="YELGAHY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22762">
        <property type="entry name" value="OTU_fungi_OTU2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049771">
        <property type="entry name" value="OTU2-like_OTU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003323">
        <property type="entry name" value="OTU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050704">
        <property type="entry name" value="Peptidase_C85-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419:SF10">
        <property type="entry name" value="DEUBIQUITINASE OTUD6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419">
        <property type="entry name" value="OTU DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02338">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50802">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OTU" evidence="2">
        <location>
            <begin position="170"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ80823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="301" mass="33383" checksum="5E3A5C751A8BAABF" modified="2010-06-15" version="1">MSEANREEMLARHRKEIRELTARIIQKKKNATKKTRRGINSECESLEHELKDRHSREIAALGNPNGEFIEAPEVPESKPQGQGKEEDTEELEEKSNTITDTPTNTIASEGTPQQSSVGPKKPNRQKARLARRAAEAAALSAEAEAEASALPNPRQAEMAAMQTLLRNLHLGEKEIAPDGHCLYSAFADQLPMPSDYKTTREKCAEYMLAHRDEFEPFLEEPIEEHVRKVRETAEWGGQAEVLALAKAFGVVVNIVQAEGRGVEKMNEGGAEGEVWLGYYRHSFGLGEHYNSLKKVGAKGGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8P8</accession>
    <name>D5G8P8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00003071001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="FN430049">
        <property type="protein sequence ID" value="CAZ80891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836700.1">
        <property type="nucleotide sequence ID" value="XM_002836654.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8P8"/>
    <dbReference type="FunCoup" id="D5G8P8">
        <property type="interactions" value="715"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G8P8"/>
    <dbReference type="GeneID" id="9186258"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003071001"/>
    <dbReference type="eggNOG" id="KOG0922">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001832_5_11_1"/>
    <dbReference type="InParanoid" id="D5G8P8"/>
    <dbReference type="OMA" id="APECHEN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005684">
        <property type="term" value="C:U2-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000390">
        <property type="term" value="P:spliceosomal complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000001">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000101">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000191">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF85">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="32"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="213"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ80891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="676" mass="75580" checksum="AE3F76F787D5A536" modified="2010-06-15" version="1">MSKNMSFGKITNKTIKEQRESLPVFKLRSSLIKAVQGNQLLIVVGDTGSGKTTQMTQFLAEAGFADNGMIGCTQPRRVAAMSVAKRVAEEVGCRVGQEVGYTIRFEDCTGPETKIKYMTDGMLQREVLLDPDLRRYSVIILDEAHERTIATDVLFGLLKKTLKRRADLKLIVTSATLDAEKFSNYFNQCPIFTIPGRTYPVEILYTKEPESDYLDAALITVMQIHLSEPPGDILLFLTGQEEIDTSCEILYERMKALGPSVPELIILPVYSALPSEMQSKIFEPAPPGCRKVVIATNIAETSITIDQIYYVIDPGFVKQNAFDPKLGMDSLVVTPISQAQAKQRAGRAGRTGPGKCYRLYTEAAFQSEMLPTSIPEIQRQNLSHTILMLKAMGINDLLHFDFMDPPPTNTMLTALEELYALSALDDEGLLTRLGRKMADFPMEPALAKVLIASVDIGCSDEILSIVAMLSVQTVFYRPKEKQNQADQKKAKFHDPHGDHLTLLNVYNAWKNSAFSNPWCFDNFIQARSMKRAKDVRAQLEMIMSRYNHRVVSCGRDTMRVRQALCSGFFRNSARKDPQEGYKTLIEGTPVYMHPSSALFGKPAEHYMHCTTAIEPKWLVDCAPSFFKVGGGKGELSKRRRNERIQPLFNRFGDGDDSWRLSAQKRQGRGGGGGTWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8S7</accession>
    <name>D5G8S7_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glycine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Diadenosine tetraphosphate synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00003052001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.14" evidence="2"/>
    <dbReference type="EMBL" id="FN430052">
        <property type="protein sequence ID" value="CAZ80920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836729.1">
        <property type="nucleotide sequence ID" value="XM_002836683.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8S7"/>
    <dbReference type="FunCoup" id="D5G8S7">
        <property type="interactions" value="1056"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G8S7"/>
    <dbReference type="GeneID" id="9186279"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003052001"/>
    <dbReference type="eggNOG" id="KOG2298">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015515_1_2_1"/>
    <dbReference type="InParanoid" id="D5G8S7"/>
    <dbReference type="OMA" id="MEMQYFV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004820">
        <property type="term" value="F:glycine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070150">
        <property type="term" value="P:mitochondrial glycyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00774">
        <property type="entry name" value="GlyRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00858">
        <property type="entry name" value="GlyRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.800:FF:000004">
        <property type="entry name" value="Glycine--tRNA ligase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027031">
        <property type="entry name" value="Gly-tRNA_synthase/POLG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033731">
        <property type="entry name" value="GlyRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002315">
        <property type="entry name" value="tRNA-synt_gly"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00389">
        <property type="entry name" value="glyS_dimeric"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003211">
        <property type="entry name" value="PRK04173.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10745:SF0">
        <property type="entry name" value="GLYCINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10745">
        <property type="entry name" value="GLYCYL-TRNA SYNTHETASE/DNA POLYMERASE SUBUNIT GAMMA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01043">
        <property type="entry name" value="TRNASYNTHGLY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="9">
        <location>
            <begin position="192"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ80920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="609" mass="68217" checksum="E58B587E0631FEEE" modified="2010-06-15" version="1">MATTKTGAPLDRGTLDTLLRRRLFYTPAFELYGGVSGLYDYGPPGCSLQANIVDAWRKHFVHEEDMLEVDCTMLTPNDVLKTSGHVDKFADWMCKDPKTGEIFRADHIIEEILEARLKGDKEARGERIVEEGEDADKKKRKKKLKDIKAIKLDEKVIQEYEGVLAKISDCSGEELGKLIVDYGITSPTTGNPLEPPVSFNLMFQTSIGPSANLPGYLRPETAQGQFLTFNKLYEFNNLRMPFASASIGKSFRNEISPRSGLLRTTIVEKTIGEAVKEGIVDNETLGYFLARIHLFLKELGVDQEKVRFRQHMPNEMAHYAADCWDAELFTSYGWIECVGCADRSAYDLTVHSSRTGEQLVVRETLEPPKVWEEWDMDVDKKWLGPKFRKDAKKIEEAVARISQGAREKLASVQKDGGKVFIEVEGLGSVELEKVTIAKNKKAVNIREYTPNVIEPSFGIGRIFYSLIEHIYWSRPGDEARGVLSFPPKIAPTKILIVPLSGHSDFAPIVTALSAKIRRAGISNRVDNSSATIGKRYARNDELGTPFGVTVDFQSLKDGTITLRERDGMKQVRGTQDEVIETVSQILDGDITFDDALAKFGEFQGQELDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8Y2</accession>
    <name>D5G8Y2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">3-dehydrosphinganine reductase</fullName>
            <ecNumber evidence="9">1.1.1.102</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00004893001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the reduction of 3'-oxosphinganine (3-ketodihydrosphingosine/KDS) to sphinganine (dihydrosphingosine/DHS), the second step of de novo sphingolipid biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>sphinganine + NADP(+) = 3-oxosphinganine + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22640"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57817"/>
            <dbReference type="ChEBI" id="CHEBI:58299"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.102"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="11">
            <dbReference type="Rhea" id="RHEA:22642"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.1.1.102" evidence="9"/>
    <dbReference type="EMBL" id="FN430053">
        <property type="protein sequence ID" value="CAZ80975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836784.1">
        <property type="nucleotide sequence ID" value="XM_002836738.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8Y2"/>
    <dbReference type="FunCoup" id="D5G8Y2">
        <property type="interactions" value="91"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G8Y2"/>
    <dbReference type="GeneID" id="9181258"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004893001"/>
    <dbReference type="eggNOG" id="KOG1210">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_3_0_1"/>
    <dbReference type="InParanoid" id="D5G8Y2"/>
    <dbReference type="OMA" id="ICGVFEE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047560">
        <property type="term" value="F:3-dehydrosphinganine reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006666">
        <property type="term" value="P:3-keto-sphinganine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08939">
        <property type="entry name" value="KDSR-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045022">
        <property type="entry name" value="KDSR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43550">
        <property type="entry name" value="3-KETODIHYDROSPHINGOSINE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43550:SF3">
        <property type="entry name" value="3-KETODIHYDROSPHINGOSINE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0746">Sphingolipid metabolism</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00026112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ80975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="317" mass="34805" checksum="CA2D1751B9BC5261" modified="2010-06-15" version="1">MPLFSSNKLPVDGRLVVITGGSQGMGLSVAKLLASKGANVAIIARNKEKLDRAVGEIRALAAREEQIFIAVSADLTSAGEARRSLEEIRTWGGVPDIVWTCAGEAFPGFFKDQDVQLLEKQVQTNYLSVLWTAHAALNIMTQSPLPPQSPKRHLIFTSSVMAFYPLAGYNAYTPSKAAIRSLADGLRQECHLYNIAVHACFPGTIYSPGFETEQLTKPEITKILEGNDEGQSPDEVARQCVRRLEKGHALAVSSFMGETLRAASWGASPRGNWVWDTVFAWIINVVWLFMGPVMDSEVVKYRKKNGDETFYSVPRGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5G967</accession>
    <name>D5G967_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">propanoyl-CoA C-acyltransferase</fullName>
            <ecNumber evidence="2">2.3.1.176</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Propanoyl-CoA C-acyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00003170001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.1.176" evidence="2"/>
    <dbReference type="EMBL" id="FN430056">
        <property type="protein sequence ID" value="CAZ81060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836869.1">
        <property type="nucleotide sequence ID" value="XM_002836823.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G967"/>
    <dbReference type="STRING" id="656061.D5G967"/>
    <dbReference type="GeneID" id="9184383"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003170001"/>
    <dbReference type="eggNOG" id="KOG1406">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035425_0_0_1"/>
    <dbReference type="InParanoid" id="D5G967"/>
    <dbReference type="OMA" id="PSLYAMM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00829">
        <property type="entry name" value="SCP-x_thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000016">
        <property type="entry name" value="Non-specific lipid-transfer protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055140">
        <property type="entry name" value="Thiolase_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020613">
        <property type="entry name" value="Thiolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006102">
        <property type="entry name" value="PRK08256.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42870">
        <property type="entry name" value="ACETYL-COA C-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42870:SF1">
        <property type="entry name" value="NON-SPECIFIC LIPID-TRANSFER PROTEIN-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22691">
        <property type="entry name" value="Thiolase_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00737">
        <property type="entry name" value="THIOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="6" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="7" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Thiolase N-terminal" evidence="9">
        <location>
            <begin position="8"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase C-terminal" evidence="10">
        <location>
            <begin position="265"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF22691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ81060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="459" mass="49254" checksum="13B16200C3657966" modified="2010-06-15" version="1">MTSSGSPVYVLGVGMTKFIKPRGQVDYPEMGYEAGIKALADAGVNYDDVDTGIACYAYGDSTSGQRVFYQFGMTGIPVYNVNNNCSTGATGLHMARRFLAHGAGNVALVVGFEKMLPGSLQSFFSDRTNPLDKSYVMMANTRGVDPKRPGAVQFFGNAGREYMERFGATKEDFAEIARVNHEHSRRNPYSQFRDVYTLEQIMGSPEIWDPLTKLQCCPTSDGAAAAVLVGEKWLQEHPELRERAVLIAGQAMATDSPDLYSKSAIDLIGFAMTKSAAEQALSEANLAPNAIKLVELHDCFSTNELITLDALSLCPPGKAHEMVRNGDITYGGRVVVNPSGGLISKGHPLGATGLAQCAELVWQLRGWAAERTVEGVGAALQHNLGLGGAAVVTVYKRADGSKNRRLSDNELERVTGISGRGYNPAVEARAVTQSEIEVGRSRRAASGWMGGGVVGETRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>D5G986</accession>
    <name>D5G986_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00003195001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the intradiol ring-cleavage dioxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430056">
        <property type="protein sequence ID" value="CAZ81079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836888.1">
        <property type="nucleotide sequence ID" value="XM_002836842.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G986"/>
    <dbReference type="GeneID" id="9184365"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003195001"/>
    <dbReference type="eggNOG" id="ENOG502QWDJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046727_2_0_1"/>
    <dbReference type="InParanoid" id="D5G986"/>
    <dbReference type="OMA" id="RHEFILV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018576">
        <property type="term" value="F:catechol 1,2-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009712">
        <property type="term" value="P:catechol-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.130.10">
        <property type="entry name" value="Aromatic compound dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007535">
        <property type="entry name" value="Catechol_dOase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000627">
        <property type="entry name" value="Intradiol_dOase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015889">
        <property type="entry name" value="Intradiol_dOase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050770">
        <property type="entry name" value="Intradiol_RC_Dioxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33711">
        <property type="entry name" value="DIOXYGENASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G02910)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33711:SF7">
        <property type="entry name" value="INTRADIOL RING-CLEAVAGE DIOXYGENASES DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00775">
        <property type="entry name" value="Dioxygenase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04444">
        <property type="entry name" value="Dioxygenase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49482">
        <property type="entry name" value="Aromatic compound dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Catechol dioxygenase N-terminal" evidence="8">
        <location>
            <begin position="21"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="Intradiol ring-cleavage dioxygenases" evidence="7">
        <location>
            <begin position="96"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ81079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="304" mass="33589" checksum="CDC4471B08D55F47" modified="2010-06-15" version="1">MDSTEENITASTVAMGSQCKDERLQYLMERLVTHLHDFARETQLTTQEWMKALIFLKDALSPPHPLPISNVLGLSTLVDSINNPKSANATESSVLGPFYTDNAHFTENGASIASQNRGEPCLVRCTLKDTSGNPIPDATIDVWEADETGRYDTQYEDAAGPDCRGVLKSNEDGEFWFKCVKPVSYPIPHDGPVGELLGVLGRHPFRPAHLHFKIEKEGFDQLITHLPPALYLRGDPYETSDAVLGVKSSLLVTPQKVQDPEIAARYGISVSDWDILWDFVLVGEAEGRQLKKDRSGKTGYTVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>D5G991</accession>
    <name>D5G991_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00003201001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430056">
        <property type="protein sequence ID" value="CAZ81084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836893.1">
        <property type="nucleotide sequence ID" value="XM_002836847.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G991"/>
    <dbReference type="FunCoup" id="D5G991">
        <property type="interactions" value="161"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G991"/>
    <dbReference type="GeneID" id="9184370"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003201001"/>
    <dbReference type="eggNOG" id="KOG1946">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046065_1_1_1"/>
    <dbReference type="InParanoid" id="D5G991"/>
    <dbReference type="OMA" id="KVWQYIR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd10567">
        <property type="entry name" value="SWIB-MDM2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.245.10">
        <property type="entry name" value="SWIB/MDM2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014876">
        <property type="entry name" value="DEK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019835">
        <property type="entry name" value="SWIB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036885">
        <property type="entry name" value="SWIB_MDM2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003121">
        <property type="entry name" value="SWIB_MDM2_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13844">
        <property type="entry name" value="SWI/SNF-RELATED MATRIX-ASSOCIATED ACTIN-DEPENDENT REGULATOR OF CHROMATIN SUBFAMILY D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08766">
        <property type="entry name" value="DEK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02201">
        <property type="entry name" value="SWIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00151">
        <property type="entry name" value="SWIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47592">
        <property type="entry name" value="SWIB/MDM2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51925">
        <property type="entry name" value="SWIB_MDM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DM2" evidence="2">
        <location>
            <begin position="171"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ81084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="235" mass="26457" checksum="3F2603012675EBCD" modified="2010-06-15" version="1">MASSALSDEDVSTFIPIIDAILFESDLTTVSVKKVRNGLQTKVGYDKQIDILIADRFENVLAQREFDNGVKQEEDAEGEDEDKYEPEVKGEVFEDGFSTAVDFENAVKHELAAELHAQWNSGRPSRSTKKKSVKPAPKVRKSKMSDVKVKSDRSGGERPAKKKRKANPNSPFMAPLILSEPLSGLLGETMLSRPETVKRIWAYVKERNLQDPEDKRYILCDDSMKPIFGNKIHML</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9D9</accession>
    <name>D5G9D9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Gluconokinase</fullName>
            <ecNumber evidence="3 9">2.7.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00003264001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>D-gluconate + ATP = 6-phospho-D-gluconate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19433"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18391"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58759"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Carbohydrate acid metabolism; D-gluconate degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the gluconokinase GntK/GntV family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.12" evidence="3 9"/>
    <dbReference type="EMBL" id="FN430059">
        <property type="protein sequence ID" value="CAZ81132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836941.1">
        <property type="nucleotide sequence ID" value="XM_002836895.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9D9"/>
    <dbReference type="FunCoup" id="D5G9D9">
        <property type="interactions" value="1010"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G9D9"/>
    <dbReference type="GeneID" id="9182883"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003264001"/>
    <dbReference type="eggNOG" id="KOG3354">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077168_0_1_1"/>
    <dbReference type="InParanoid" id="D5G9D9"/>
    <dbReference type="OMA" id="HFIYLRA"/>
    <dbReference type="UniPathway" id="UPA00792"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046316">
        <property type="term" value="F:gluconokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009051">
        <property type="term" value="P:pentose-phosphate shunt, oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02021">
        <property type="entry name" value="GntK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006001">
        <property type="entry name" value="Therm_gnt_kin"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01313">
        <property type="entry name" value="therm_gnt_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43442">
        <property type="entry name" value="GLUCONOKINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43442:SF3">
        <property type="entry name" value="GLUCONOKINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13671">
        <property type="entry name" value="AAA_33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ81132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="213" mass="24181" checksum="417BB73734DC12B3" modified="2010-06-15" version="1">MLSYNEKSGVYVSTPSAIHTMGKVQSIPKHETRLRHIWIITGPAGCGKSSVADFLAKRLNLPYIEGDDFHPRSNVEKMANGIPLTDDDRWDWLETLRNKAVEELQSGAEGCVVTCSCLKRRYRDVIRIAGREADDVVVRFVYLRASEELLLKRVRARKNHYMKDDMVHSQFTALEEPDESEVDVVSVDVSGSLASVQNLTVERIRGCMEEERQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9G7</accession>
    <name>D5G9G7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00003393001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430061">
        <property type="protein sequence ID" value="CAZ81160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836969.1">
        <property type="nucleotide sequence ID" value="XM_002836923.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9G7"/>
    <dbReference type="GeneID" id="9182911"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003393001"/>
    <dbReference type="HOGENOM" id="CLU_2428661_0_0_1"/>
    <dbReference type="InParanoid" id="D5G9G7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="91" mass="10614" checksum="126F23F0D9F2CF45" modified="2010-06-15" version="1">MKMKLDNNTPMQSKVSMIHDLSYIPYCVPSHHHFHKNQAPTPASKQANKQREEGGKTKPSETEWHQKIQKIQNRNKSENSHTVSSRKHTLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9M2</accession>
    <name>D5G9M2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00003325001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430063">
        <property type="protein sequence ID" value="CAZ81215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837024.1">
        <property type="nucleotide sequence ID" value="XM_002836978.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9M2"/>
    <dbReference type="FunCoup" id="D5G9M2">
        <property type="interactions" value="38"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G9M2"/>
    <dbReference type="GeneID" id="9185358"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003325001"/>
    <dbReference type="eggNOG" id="KOG4812">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041193_0_0_1"/>
    <dbReference type="InParanoid" id="D5G9M2"/>
    <dbReference type="OMA" id="SFVWNGM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030001">
        <property type="term" value="P:metal ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031398">
        <property type="term" value="P:positive regulation of protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22212">
        <property type="entry name" value="NDFIP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019325">
        <property type="entry name" value="NEDD4/Bsd2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13396:SF5">
        <property type="entry name" value="NEDD4 FAMILY INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13396">
        <property type="entry name" value="NEDD4 FAMILY INTERACTING PROTEIN 1/2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10176">
        <property type="entry name" value="NEDD4_Bsd2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="186"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="280"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="240"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="50"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="81"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ81215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="333" mass="35523" checksum="259433F95C974E07" modified="2010-06-15" version="1">MGPRYEKIAAEDDEDLDIHGNITTGSSVSISSRPGVTTIQIPNSPPPSFHSRSGTPDSDSALSESFDSLSDGEDDDMRNVRGSQNSNFNHESIPLAPSAMTGLSAPVSSASTFAAMPGRAVGGGQTDGVFANLSAKPTVAEKLEEHPPSYEQAAADATPPYWETTILAPGMSADEVFIDGLPVGSVFAFIWNAMISMSFTFVGFLLTYLLHTTHAAKNGSRAGLGITLIQYGFYMRSRPDDYTGGAPPPENEPSEPNSYDFGVADMVTDTPEDGIGRNEWISYALMVVGWFILIRSVSSFMRARRTEQVVLASPDRGLGTAVIADDEQPARAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9V9</accession>
    <name>D5G9V9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00005096001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430064">
        <property type="protein sequence ID" value="CAZ81302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837111.1">
        <property type="nucleotide sequence ID" value="XM_002837065.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9V9"/>
    <dbReference type="GeneID" id="9188545"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005096001"/>
    <dbReference type="HOGENOM" id="CLU_2074857_0_0_1"/>
    <dbReference type="InParanoid" id="D5G9V9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="118" mass="13326" checksum="A18F73DAB300C2CB" modified="2010-06-15" version="1">MIAQKSGQPYGKFTPYNARYVCAGLSPTPRLLRYRKYHLALFSTIKRGGSLDVVEYLANNTCSWTHPACLRVKYHRSSRTNGERGSTGSRMFPGGRKSARILLPRKSDSIPRHLPLKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAA5</accession>
    <name>D5GAA5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00005203001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the UreF family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430075">
        <property type="protein sequence ID" value="CAZ81459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837268.1">
        <property type="nucleotide sequence ID" value="XM_002837222.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAA5"/>
    <dbReference type="GeneID" id="9185741"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005203001"/>
    <dbReference type="eggNOG" id="ENOG502REVQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049215_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAA5"/>
    <dbReference type="OMA" id="WVGRHEK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4190.10">
        <property type="entry name" value="Urease accessory protein UreF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01385">
        <property type="entry name" value="UreF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002639">
        <property type="entry name" value="UreF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038277">
        <property type="entry name" value="UreF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33620">
        <property type="entry name" value="UREASE ACCESSORY PROTEIN F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33620:SF1">
        <property type="entry name" value="UREASE ACCESSORY PROTEIN F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01730">
        <property type="entry name" value="UreF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="1" id="KW-0996">Nickel insertion</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00046339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ81459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="293" mass="31668" checksum="8207E37339FD4F6E" modified="2010-06-15" version="1">MGEDADNRALREEIAGLESRLRAARAKLPSKTETRSHGKATVPLSVLSIHSCKLCATVATPETHFLLLLSDSALPLGSFAFSGGLESYTAHRPSTSTPRETTERFLRLSVSSLSRAALPFVLEAHREPEAALTLDDVFDASTICTVARRASVSQGRALLTVHEKSFSSSLPPSPELEAYRRALRAENVRDRASGHFPVVYGLLCNLAGISLEGTGYLFLLGHVKAVLSAAVRQSLIGPYHMQALLAAESTRRIMDDALTVAKGIAVEDAGQSVPTLDLYQGRHELLYSRVFNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAL4</accession>
    <name>D5GAL4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00003670001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430079">
        <property type="protein sequence ID" value="CAZ81557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837366.1">
        <property type="nucleotide sequence ID" value="XM_002837320.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAL4"/>
    <dbReference type="STRING" id="656061.D5GAL4"/>
    <dbReference type="GeneID" id="9187497"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003670001"/>
    <dbReference type="HOGENOM" id="CLU_306783_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAL4"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13275:SF4">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 72 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13275">
        <property type="entry name" value="YL-1 PROTEIN TRANSCRIPTION FACTOR-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="938"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="domain" description="FHA" evidence="3">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="240"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="443"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="543"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="764"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="899"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="138"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="175"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="273"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="305"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="337"/>
            <end position="364"/>
        </location>
    </feature>
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        <location>
            <begin position="365"/>
            <end position="377"/>
        </location>
    </feature>
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        <location>
            <begin position="445"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="547"/>
            <end position="557"/>
        </location>
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    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="604"/>
            <end position="614"/>
        </location>
    </feature>
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        <location>
            <begin position="620"/>
            <end position="636"/>
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        <location>
            <begin position="644"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="840"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="909"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="926"/>
            <end position="937"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ81557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="965" mass="105531" checksum="C5742B07235E0C61" modified="2010-06-15" version="1">MSRSHAEVRLLVPSRKIVIKDMKSMHGTYVNGLQLAGDETAELTDGTEVTFGSEVTRGDEIFPPKTFRCGVEWEEIPLPKPVAEPEPAAETPKSRGGYGICEDLLISDYEVSEEEGEEEEGDDELDPKFHRLEEEKTSGSGVSSTPGTNSAAPGPGQTTFVGASTSYMIPDDELPISHERSTKDMSVESLTQSKDRVLQLKRKIDEMTGSGERDVVEVNAELVDIEICDVPARPGVTIGSLMNQAQESSVSRNDKSKDVTNESGGSNMPECLVIDDEESDLEIIPEEAFKRYHHTSRTREHAQNDEIENSDMDESDDEDTSCRARVRSRRFNYVSSDESENEDEDEDDEEDDEDLEEEEQDGEDGGQHENAVGEHTGKASTANDTVDVTMVADNLYSPNAPTPVPPPEVLSSKPQVVAAPLGGIPQSKTNFAPFEELANAAVAAQNGSDSSNNPRGNVAASATIPEPPTLPPLTMLPTEAVAKKTPSGASHIHWFAEDPHTTTDSFQKRYNNSSMEQKKEYFTARQINLANLANRPNDVLSYLSPVRGNSKSPSVSQPLPPASLPWVQTFKTAEGPKEPTGLFPKVWEDRQQDSQAPANKSDKPPAPSPPPPTVTPQAKRLLEDAVAEGEKFRGLDKSTWWLGDEAEISIDETSEMVWDDEYCNDEDGEYDEEDEEEDEEENGNTPDDIPEQEMTDGIVDYEEEDEEEGEGDVMEEEDDTDSRHPTCTWFGQRKAFNTGYERFPVHHPNAGRGDFSKSRGFSISNLVHENNGPLTPPGPLMVATASSPPPGPSANGLISPPSTETKKEKSVADLLNPSSPSTGSKRKHSLLSDITPEEKSQEEEEVAHESPENMDETDDDVEEEDKMDSMTKVSTQELAMLFPGKNVQALEEEMETITLEPALPTPDGTSDEEEAIQAGEEEERPRKRARTTRPKKQFAKLAATALAGAIVGGVGVFAALVASAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAP1</accession>
    <name>D5GAP1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00003710001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430083">
        <property type="protein sequence ID" value="CAZ81584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837393.1">
        <property type="nucleotide sequence ID" value="XM_002837347.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAP1"/>
    <dbReference type="GeneID" id="9187751"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003710001"/>
    <dbReference type="HOGENOM" id="CLU_2470703_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAP1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ81584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="88" mass="9785" checksum="41B99B38BA1E726E" modified="2010-06-15" version="1">MDVLPILSSLSAGNITFCVEVGTVLHAVGRFRCLRCLVEASFSSRLRLSSAKTSPRSNRQAVSLLRCRRSAFPFAVICRISSLRWQLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAP2</accession>
    <name>D5GAP2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00003713001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430083">
        <property type="protein sequence ID" value="CAZ81585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837394.1">
        <property type="nucleotide sequence ID" value="XM_002837348.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAP2"/>
    <dbReference type="FunCoup" id="D5GAP2">
        <property type="interactions" value="52"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GAP2"/>
    <dbReference type="GeneID" id="9187752"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003713001"/>
    <dbReference type="eggNOG" id="ENOG502S328">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107716_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAP2"/>
    <dbReference type="OMA" id="TPWHEAF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01443">
        <property type="entry name" value="Cdc25_Acr2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10828">
        <property type="entry name" value="M-PHASE INDUCER PHOSPHATASE DUAL SPECIFICITY PHOSPHATASE CDC25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10828:SF50">
        <property type="entry name" value="REDUCTASE (ARC2), PUTATIVE (AFU_ORTHOLOGUE AFUA_6G13400)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="2">
        <location>
            <begin position="42"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ81585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="155" mass="17176" checksum="949C83CC6FC045D1" modified="2010-06-15" version="1">MSTPEQPWHAAYPKPISEAVFLPRSTVLEWFSSNDKLPGKYFVLVDLRRNDHEGGTIKTSINLPAQSMHPSIPALYTLFSSAGVSAIVFYCGSSQGRGARAASWMQDYINEKGDSNMKGFALEGGVKGWVKAGGKLLEMMDEYEEEAWKEPAKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAQ6</accession>
    <name>D5GAQ6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00003737001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430085">
        <property type="protein sequence ID" value="CAZ81599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837408.1">
        <property type="nucleotide sequence ID" value="XM_002837362.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAQ6"/>
    <dbReference type="FunCoup" id="D5GAQ6">
        <property type="interactions" value="16"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GAQ6"/>
    <dbReference type="GeneID" id="9187766"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003737001"/>
    <dbReference type="eggNOG" id="KOG4781">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052827_0_1_1"/>
    <dbReference type="InParanoid" id="D5GAQ6"/>
    <dbReference type="OMA" id="FIEPKNA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03443">
        <property type="entry name" value="PaaI_thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052061">
        <property type="entry name" value="PTE-AB_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47260:SF7">
        <property type="entry name" value="THIOESTERASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_1G10800)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47260">
        <property type="entry name" value="UPF0644 PROTEIN PB2B4.06"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioesterase" evidence="1">
        <location>
            <begin position="195"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="304" mass="32916" checksum="1ABE2A3758D2ECA9" modified="2010-06-15" version="1">MVILNRSPALAGRVLRHSTPLISPLRYFPTAAAIATPASRFATTKSFEPIGKSAYQKFSDKIKEATFIRPSIWLLSGVTITILTTCSYIMAAQGYIALTDEETLQAYTPTDPQEIEANNYISNHPLVTSLRSNPAFSETRPTLKIADKYKHLSFTAGTLAGPGKIVVPPLTFVEDGGKSMVQVFYLGEQLCGHPGYIHGGMLATILDEGLARCCFAALPNKIAMTASLTVNYRKPSPAGSYVVLKAKTVKVEGRKAWVEGRIETLVGEGEEASVLVEASALMIEPKQAATLKKIIRDGKLEVRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5GB68</accession>
    <name>D5GB68_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00003767001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430087">
        <property type="protein sequence ID" value="CAZ81761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837570.1">
        <property type="nucleotide sequence ID" value="XM_002837524.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GB68"/>
    <dbReference type="FunCoup" id="D5GB68">
        <property type="interactions" value="20"/>
    </dbReference>
    <dbReference type="GeneID" id="9181471"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003767001"/>
    <dbReference type="eggNOG" id="ENOG502QPU8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009024_0_1_1"/>
    <dbReference type="InParanoid" id="D5GB68"/>
    <dbReference type="OMA" id="AACRYFA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050295">
        <property type="term" value="F:steryl-beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904462">
        <property type="term" value="P:ergosteryl 3-beta-D-glucoside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000131">
        <property type="entry name" value="Glycoside hydrolase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000174">
        <property type="entry name" value="YIR007W-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041036">
        <property type="entry name" value="GH5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018087">
        <property type="entry name" value="Glyco_hydro_5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052066">
        <property type="entry name" value="Glycosphingolipid_Hydrolases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31308">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31308:SF5">
        <property type="entry name" value="ERGOSTERYL-BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18564">
        <property type="entry name" value="Glyco_hydro_5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00659">
        <property type="entry name" value="GLYCOSYL_HYDROL_F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 5 C-terminal" evidence="4">
        <location>
            <begin position="620"/>
            <end position="703"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF18564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ81761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="732" mass="82621" checksum="47572B0F513E4D68" modified="2010-06-15" version="1">MPSFLSVDGPYFRDEHNRQVTLRGVNVASDAKMPTKPNLLSHVLEGFFDGDNVSFVGRPFTLEDADVHFRRLRNCGYNVIRYIYTWEAIEARGPGIYDDEWIESTVAMLRKAKEYDFMIFMDSHQDVWSRFSGGSGAPMWTLYAMGLNPQTFDVTEAALVHNTWPDPATFPKMIWSTNYQRLACQTIFTMFFAGRDFAPKCIIDGKNIQDYLQEHFISAVAYLAKKIHEAGDLEDTTVIGWESLNEPNRGLIGYQRLDVIPPEQKLRKGTSPTAWQAILTGSGRACEIDTWDFGGTGPYKTGSALVDPEGVQAWLPADYDDSRYGWKRDPGWKLGECIWAQHGVWDPATDTILKNDYFGKMPNGELLSPEKFTETYFLDHYRKYAAAIRSIHKSTIMFCQPPVFEIPPVIKDTPDAHGRMVYAPHFYDGITLMTKKWNRLWNIDVLGVLRGRYLAPGFALKIGETAIRNCLRDQLKAMKQEGVDNMGLTPCVFTEIGVPYDMDDKYAYKTGDYISQIRAMDANHYALEGAQVGFTLWTYCVENNHEWGDQWNGEDLSIFSLDDRPPNSSATTNDSTTIVDDTNLKRALTTSTMSTTRTDLQKVTPDAVAAGSRAAEAFVRPSPVYTAGTIVESGFTLSSATFDLKLVAGKSTEETTPTEVFVPPFHFPKDGTAVTVSGGKWEYDTERSVLRWWHAEGEQWIKIIGVGRMSADYDDEGYAETCKRNYRACIVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBC9</accession>
    <name>D5GBC9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00000431001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430091">
        <property type="protein sequence ID" value="CAZ81822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837631.1">
        <property type="nucleotide sequence ID" value="XM_002837585.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBC9"/>
    <dbReference type="STRING" id="656061.D5GBC9"/>
    <dbReference type="GeneID" id="9184395"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000431001"/>
    <dbReference type="eggNOG" id="ENOG502RZHE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_324381_0_0_1"/>
    <dbReference type="InParanoid" id="D5GBC9"/>
    <dbReference type="OMA" id="WHIGAPA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="304"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="385"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="537"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="625"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="780"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="329"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="541"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="657"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="671"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="696"/>
            <end position="708"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ81822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="808" mass="87560" checksum="39DE232A45EDAEB3" modified="2010-06-15" version="1">MCYLSSILSRWDTDLSSIAACYNTITNHGHSFSLILFIIVSKLIPSRKAKYLTLRRYCSSRALTAHPSAVVSFAISCDSNLILSCSANPPVIQVHNRLLATTIAVAPRASTTSVNICVFHPTHRNIFVLAFNDGVLAAYDYSKISGGGSARRDHGTGIGQGDLKEIHTFRHLHDPSVAGSSGITGVQFLPGYRSRAVTVGEDGRAFLVDFGMRDTLGSWHTGAPATSLTVRSTAAKTGDRDDPKAYLIAVGSVHGRCYVYDGHGNKVGEQVVDVAGGKILGVEWVAGDICIPLNSGTCDPNLYSHNRSRPGSEPPAKVREGLRARTGSRLATKNRNPSRSSEPQPPIPGGKDIGDSFSKLGAGAGLERNDVEEVTNQGYMRLFSPVKKKRPLRASPTNEKKSTSAAQNLDPQERALRMETQRTSISAPPLWSGSGEKTPQPLAPVIRNDGPGQTPNTVTKTEGKTVHKQGRDWRYTKTTQSSVTVKHVSSGTSKGQPVTDKTRDGKFLSDIRSVRARVMGQTGKPVRGNLALFAPYMPGKSKGERPRSPEVVREKRISPGRLAPDPRPETSSGARVVRAMDGSNNEEKPTTQAADEAVTTVADNEATAVDDGSIEADIWLAEGKEARRSRKRKQSNADQAPETLLGLDDSAGGRRSLTGSFKSRKTVSWQDEARMTRDIPIGHDTEDHSFLPKLSGSGSPKPNSSRQDISPPPHHPPITGSFSGSAPFSIVGMQLPGAENAELRSALHNMVMEMQIALKSEITDMRAEMSERFRQQDIILRNIGHEVQMVRQENQNLRNRLSEALDTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBH2</accession>
    <name>D5GBH2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00000498001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430095">
        <property type="protein sequence ID" value="CAZ81865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837674.1">
        <property type="nucleotide sequence ID" value="XM_002837628.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBH2"/>
    <dbReference type="STRING" id="656061.D5GBH2"/>
    <dbReference type="GeneID" id="9184439"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000498001"/>
    <dbReference type="eggNOG" id="ENOG502QQPX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007526_0_2_1"/>
    <dbReference type="InParanoid" id="D5GBH2"/>
    <dbReference type="OMA" id="LAYWIEA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05154">
        <property type="entry name" value="ACAD10_11_N-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052898">
        <property type="entry name" value="ACAD10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041726">
        <property type="entry name" value="ACAD10_11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002575">
        <property type="entry name" value="Aminoglycoside_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47829:SF1">
        <property type="entry name" value="HAD FAMILY PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47829">
        <property type="entry name" value="HYDROLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G12880)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01636">
        <property type="entry name" value="APH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="28"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="372" mass="41091" checksum="369AD27874EC3EC9" modified="2010-06-15" version="1">MAGPIRHPIPLDSLTDYIRQNVPVISLPLTIQQFGYGQSNPTYLLTSSNTGAKYVLRKKPPGKLLSKTAHQVEREYKVLKALSEAAQDLPVPKVYCLCEDADVAGSAFYIMEFLDGRIFEDPALPRVTPEERAVMWKSAIVTLARLHKIRPEEHAALKSFGKPSGFYGRQVRTLSTIEAAQALVKDVQTGVEIGKIANFDEAMKFFSTTQPKDRSTVVHGDYKIDNLVFHKTEPRVIGILDWELSTIGHPLSDVSNLLSPYQLALEAGASVMFGETKGLIPGATPGLPSRQQVIEWYTQASGWNILPSEMYWGDAFGFCRNAVIVQGITARAARRQASSAKAGTYSERTPKLVALAWKYVSIDLLHLRSLGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBH5</accession>
    <name>D5GBH5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00000502001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430096">
        <property type="protein sequence ID" value="CAZ81868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837677.1">
        <property type="nucleotide sequence ID" value="XM_002837631.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBH5"/>
    <dbReference type="GeneID" id="9184449"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000502001"/>
    <dbReference type="HOGENOM" id="CLU_2869279_0_0_1"/>
    <dbReference type="InParanoid" id="D5GBH5"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ81868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="64" mass="7003" checksum="4EC1D3D1C976666B" modified="2010-06-15" version="1">MASPRVMPIYLSRALSGYIAQGGSTRAESLSLERELPTNNTIGVDNSARTPEKHRAILGRYKVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5GC22</accession>
    <name>D5GC22_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cyanate hydratase</fullName>
            <shortName evidence="5">Cyanase</shortName>
            <ecNumber evidence="5">4.2.1.104</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Cyanate hydrolase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Cyanate lyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cyn1</name>
        <name evidence="7" type="ORF">GSTUM_00005779001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 5">Catalyzes the reaction of cyanate with bicarbonate to produce ammonia and carbon dioxide.</text>
    </comment>
    <comment type="function">
        <text evidence="4">Transcriptional coactivator that stimulates GCN4-dependent transcriptional activity by bridging the DNA-binding region of GCN4 and TBP (SPT15), thereby recruiting TBP to GCN4-bound promoters. Involved in induction of the ribosome quality control (RQC) pathway; a pathway that degrades nascent peptide chains during problematic translation. Required to prevent stalled ribosomes from frameshifting.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>cyanate + hydrogencarbonate + 3 H(+) = NH4(+) + 2 CO2</text>
            <dbReference type="Rhea" id="RHEA:11120"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29195"/>
            <dbReference type="EC" id="4.2.1.104"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MBF1 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the cyanase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.104" evidence="5"/>
    <dbReference type="EMBL" id="FN430098">
        <property type="protein sequence ID" value="CAZ82065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837874.1">
        <property type="nucleotide sequence ID" value="XM_002837828.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GC22"/>
    <dbReference type="STRING" id="656061.D5GC22"/>
    <dbReference type="GeneID" id="9181334"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005779001"/>
    <dbReference type="eggNOG" id="ENOG502S3YJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103452_0_0_1"/>
    <dbReference type="InParanoid" id="D5GC22"/>
    <dbReference type="OMA" id="YELVMIN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008824">
        <property type="term" value="F:cyanate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        </source>
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        <source>
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        <source>
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    <sequence length="156" mass="17573" checksum="89369C4692506A09" modified="2010-06-15" version="1">MSSIETYQNLPPVTDILFKQKKAKGLSFEKLGELLGRDEVAVAAIFYGQAHASLDDIKKLAEALELNEVVVANDLEGFPDRGRTLDMPPREPLIYRLYEIVQNYGLAYKAILNEKFGDGIMSAIAFTTKVEKETDDKGVDWAVITLRGKWLPYSRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GC90</accession>
    <name>D5GC90_TUBMM</name>
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            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
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        <name evidence="3" type="ORF">GSTUM_00000578001</name>
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            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
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            <taxon>Tuber</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
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                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
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                <person name="Lebrun M.H."/>
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                <person name="Wincker P."/>
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            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3 4">Mel28</strain>
        </source>
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        <property type="component" value="Unassembled WGS sequence"/>
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    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ82133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="348" mass="39168" checksum="2CC5EC9859F3DCA1" modified="2010-06-15" version="1">MLQSQQQQIFQNHQHFQPHDQNNWHHAAQQQQAQQQQPQQQQPQFGMPGRPMLTPSQPSPAPNPQLTLMGARAGTEEDPKVYGWILELMQGINRETALLELSKKREQVDDLALILWHSVGVMTALLQEIISVYPLLMPSSLTAAASNRVCNALALLQCVASHNDTRGLFLNAHIPLFLYPFLNTTSKSRPFEYLRLTSLGVIGALVKNDSSDVINFLLSTEIIPLCLRIMETGSELSKTVAIFIVQKILLDDMGLAYICQTYERFYAVGTVLSNMVTQLVDQQTVRLLKHVVRCFLRLSDNPRAREALRQCLPEPLRDATFSSVLRDDAATKRCLAQLLINLSDCVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCI9</accession>
    <name>D5GCI9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00005908001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
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                <person name="Kohler A."/>
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                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
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                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
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                <person name="Rubini A."/>
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                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
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                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430109">
        <property type="protein sequence ID" value="CAZ82232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002837995.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCI9"/>
    <dbReference type="GeneID" id="9186414"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005908001"/>
    <dbReference type="eggNOG" id="ENOG502QTDM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013895_0_1_1"/>
    <dbReference type="InParanoid" id="D5GCI9"/>
    <dbReference type="OMA" id="PTMMLKL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055509">
        <property type="entry name" value="DUF7082"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39463">
        <property type="entry name" value="MEDUSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39463:SF1">
        <property type="entry name" value="MEDUSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23305">
        <property type="entry name" value="DUF7082"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7082" evidence="2">
        <location>
            <begin position="359"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="163"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="535"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="216"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="241"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="288"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="305"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="323"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="563"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="586"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="633"/>
            <end position="666"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ82232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="698" mass="75203" checksum="BDB053A5A7A1E2DD" modified="2010-06-15" version="1">MSAYGKSPQQQAEYDTRPPQTQHLPNPQQSAYGYNHSQAHYQHSSNLSEPAYPSQSFEDQGPRVVGYRPTQGVEGGAFSVLLQSPTDLTTDSRSFRFMFGSRRCITTIRREENPGMYRLTGEIPAFSLTGWVAPQVTVYLNIEDADGQELDTFDVGTFTYTDSSAQQSFSSPPPRGSRKRKSTVEPDEASSRGPAKRPASQPIRPKSEDYGYGYPAPGPSGIPPFTPHSNDDRSYLYSGYNAEQSTQGPGYRPQTSPRAYGYAPYPAGSSQPAQAGQPWVQPYPVQPSGAQSSASRQMRSDTSPNQPPASLPSPSNTPNPTLIRTSTLQQAAHTAPPPGQGGPSGTGTGFNPYGLYPEKAVLDIQGELGDMARNWNPEEWDSRRRLVQFWRRQKGSTIHTTFRPVAPNDRQPNSICISCIWWAEKSECFVTSVDCIYLLESLIAVRFTVEEKNRIRRNLEGFRPLTVSKAKSDSENFFKLIMAFPNPKPRNIEKDVKVFPWSVLGHALKKIIGKYCASYSSTASVIPTAPGASAYPGGSVGEPSQSVAGETAVPPVPSPPTATGPTSSSSGASASLTGTTGPLPVKQSSATSSPPTHRPGGSSELPAHVPALPPPSASQQGQQPLWHPHQPLAPSQQQMSAQGQRTSWDLTSYLDPNTASGNTAGTQVVHYPHGSVAAEGRESVSGTVQQGQLPGDVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCP6</accession>
    <name>D5GCP6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00005937001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430120">
        <property type="protein sequence ID" value="CAZ82289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838098.1">
        <property type="nucleotide sequence ID" value="XM_002838052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCP6"/>
    <dbReference type="GeneID" id="9187908"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005937001"/>
    <dbReference type="HOGENOM" id="CLU_2759637_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCP6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ82289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="70" mass="8229" checksum="E89E1E48E018318B" modified="2010-06-15" version="1">MFRRKPGVHLMRKTLETDNNKEAEKRGEGRLGEYKRNNVDFYHRIIFVIMLLPGEWVARDVIPGASMLQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCR0</accession>
    <name>D5GCR0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00005976001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430120">
        <property type="protein sequence ID" value="CAZ82303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838112.1">
        <property type="nucleotide sequence ID" value="XM_002838066.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCR0"/>
    <dbReference type="STRING" id="656061.D5GCR0"/>
    <dbReference type="GeneID" id="9187922"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005976001"/>
    <dbReference type="HOGENOM" id="CLU_044897_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCR0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="340" mass="39246" checksum="E5ADEB92FEC3960A" modified="2010-06-15" version="1">MGPRADSSLSVEAPESAGGPKEVGTSRPVKTEEPTDSYPKRPFEPELSEYEDVFQNCCRFLILLDRCLTNYPKENPLITEMVKAFLQLAWEPIEMSKHRGSDLWPDGLESPWEFPPPYARFLNWGDYKNCGYGGQDIYVYAITTQVLVWRAVKSTNRLLNLVCKDQKWRKWMDDQSLDDKAIRDRAIEAFHLPGTDTGQTPVPDRFVSKIEGHVREPFPENWACIIAIPSFVEDFFFDNNKPISAWTETLRYYDKYIDSIKSQTPNTWESFMRYELAIDRDRRQELREGLEARAYSVGLFPGRPVAPDSHCPSTTAWEIVTYVLSSDHAELPHPQPRIPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCY3</accession>
    <name>D5GCY3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00000867001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430129">
        <property type="protein sequence ID" value="CAZ82376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838185.1">
        <property type="nucleotide sequence ID" value="XM_002838139.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCY3"/>
    <dbReference type="GeneID" id="9183838"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000867001"/>
    <dbReference type="eggNOG" id="ENOG502S6BE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_573892_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCY3"/>
    <dbReference type="OMA" id="WHYETES"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="409"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="52"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="409"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ82376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="476" mass="54759" checksum="47C02D4271E1E63E" modified="2010-06-15" version="1">MNQGPPETSHKQRWGLSAYQGYFSGTHKYNGQPELTSKPAEEKAFKGVIKRNVMLEAELVKLEKQKEKVDEANERLQRDNEALKTQATVLKDGIAQLKGELEESERIREAQMKELRQLQAQDYERAKGATGEPHEAISSQFREIFAQCSHWARDYFNIKMVDFDISKFPEFKRELEEVSWSNSDWGSKVLFKASHLVQAVLGNMICDQIFSSPFSGTPRGFFQQFQNMYEIKYSIDRAEAQRWRASSLQTLYSSKRWPLSSKESHSLRTCKQRHAKDIYWDIDEVLRPIITAHYPNLSDQEADVQKERLFSIVEDAKALGEKMGKQSSELRMLSKSWFSKNDRLFILDDVRMESRLGNDEYDPRDDLRVDLILSPGFLKYGNDNAENLDDWNVWTPAIVEIDVPLPPLPSRLPAPPAKTRPAFTTGYPELPRDGHKQEAASPSTTTEQEHCKDGEYEDVPVDAPMDPGPYDCLGWH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5GD06</accession>
    <name>D5GD06_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00000905001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430133">
        <property type="protein sequence ID" value="CAZ82399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GD06"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000905001"/>
    <dbReference type="HOGENOM" id="CLU_3415270_0_0_1"/>
    <dbReference type="InParanoid" id="D5GD06"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ82399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="27" mass="2957" checksum="704258273DCC8A0A" modified="2010-06-15" version="1">MDPTLVVLLESFSNYLVTKSSVPVMVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>D5GD20</accession>
    <name>D5GD20_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcription elongation factor Spt6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00000938001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Plays a role in maintenance of chromatin structure during RNA polymerase II transcription elongation thereby repressing transcription initiation from cryptic promoters. Mediates the reassembly of nucleosomes onto the promoters of at least a selected set of genes during repression; the nucleosome reassembly is essential for transcriptional repression.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the SPT6 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430136">
        <property type="protein sequence ID" value="CAZ82413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838222.1">
        <property type="nucleotide sequence ID" value="XM_002838176.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5GD20">
        <property type="interactions" value="1161"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GD20"/>
    <dbReference type="GeneID" id="9183875"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000938001"/>
    <dbReference type="eggNOG" id="KOG1856">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001680_0_1_1"/>
    <dbReference type="InParanoid" id="D5GD20"/>
    <dbReference type="OMA" id="GYFYLCF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000791">
        <property type="term" value="C:euchromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005721">
        <property type="term" value="C:pericentric heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001073">
        <property type="term" value="F:transcription antitermination factor activity, DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033554">
        <property type="term" value="P:cellular response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031440">
        <property type="term" value="P:regulation of mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140673">
        <property type="term" value="P:transcription elongation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02065">
        <property type="entry name" value="B12-binding_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09928">
        <property type="entry name" value="SH2_Cterm_SPT6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09918">
        <property type="entry name" value="SH2_Nterm_SPT6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2740:FF:000002">
        <property type="entry name" value="Transcription elongation factor Spt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000056">
        <property type="entry name" value="Transcription elongation factor Spt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.650">
        <property type="entry name" value="RuvA domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2740">
        <property type="entry name" value="Spt6, Death-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.850">
        <property type="entry name" value="Spt6, helix-hairpin-helix domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3500.10">
        <property type="entry name" value="Tex N-terminal region-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.140">
        <property type="entry name" value="YqgF/RNase H-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049540">
        <property type="entry name" value="Spt6-like_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028083">
        <property type="entry name" value="Spt6_acidic_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042066">
        <property type="entry name" value="Spt6_death-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032706">
        <property type="entry name" value="Spt6_HHH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028088">
        <property type="entry name" value="Spt6_HTH_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035420">
        <property type="entry name" value="Spt6_SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035018">
        <property type="entry name" value="Spt6_SH2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035019">
        <property type="entry name" value="Spt6_SH2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028231">
        <property type="entry name" value="Spt6_YqgF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055179">
        <property type="entry name" value="Tex-like_central_region"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023323">
        <property type="entry name" value="Tex-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023319">
        <property type="entry name" value="Tex-like_HTH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017072">
        <property type="entry name" value="TF_Spt6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037027">
        <property type="entry name" value="YqgF/RNaseH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10145">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10145:SF6">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14635">
        <property type="entry name" value="HHH_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14641">
        <property type="entry name" value="HTH_44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14633">
        <property type="entry name" value="SH2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14632">
        <property type="entry name" value="SPT6_acidic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21710">
        <property type="entry name" value="Spt6_S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22706">
        <property type="entry name" value="Tex_central_region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14639">
        <property type="entry name" value="YqgF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036947">
        <property type="entry name" value="Spt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="RuvA domain 2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158832">
        <property type="entry name" value="Tex N-terminal region-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="6 8" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="S1 motif" evidence="11">
        <location>
            <begin position="1108"/>
            <end position="1178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="1048"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="10"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="28"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="46"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="67"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="87"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="100"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="137"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="154"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="181"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR036947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ82413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1419" mass="163758" checksum="49830E523568C7AD" modified="2010-06-15" version="1">MRDSFIDREAEMDDDDELDQEEFDEDTGEVRRKADKPNKPPKDFDDSSEEEDDDDDEEAAKIREGFIVDDEEEDEEEEQADGSMVLVKKRKKKRRRSNREDEEEEGLDEDDLDLVLENTGHEIDRSSQSKFKRLKRGREDAEKRRRQHSRDLEEIFSDEEDAPGEPDDDDEGLDNRRGGENEFEDFIEEDEMSEDERGGGGSDDDMQMVRRRRRESNQHQGSYAPQRLGLREGAISDMHDIFGRGDEYDFALVTEDDPEQEEESREKELELKDVFEPSELAERMLTDEDNVIRQTDEPERFQLARRPFAHLKIGERELEEETEWISKFVLAKKNLPPHQEEPLMKTIRSVLEFIVIEQMEVPFIYQHRKDYLIHTERVPTRRHNDGETGYEIEATKLLTQDDLWEILELDLKFRAFLDKCRAYERTYSNLKIVTDTSDDMEAETGLQRTETIEQIQDLHDYIHFRYHSQLKDMAITNGEGRGNGYRRPGSSKTIYERIRNGKVYGMVRAFGLSAAQFAMNVRFDQKREYAEDPHNYPHAISDQYIDDPEFPTGESVLQAAKLMLAEELFTNPTLRRSMRDKWFTRGTIHVNVTEKGVRQIDEQHQYYSIAVHPAMYLRMMKAEQEGYVNVDVNLESEDKFTRRLYEYMTSENVSDIAESWNKERKEVVDMAMGKFKLMFQKAIKDELRTACEDSIAADCRRSYLKKLDQAPYKPKALKLGEIPRVFAFSNGYGERGRDAIVGVFRDEDGRLLETVKFTDLKDDTSRTNFIEVLNRRKPDVIAVAGFSVQTHKLVEEIRGIVEEEDINVVGDEVDDRSPTEVMLVNDEVARLYQNSDRAALDHPELPPLSRYCVALAKYVQNPLLEYAALGKDIVSIAFHPAQQLLPEHKLQKALETAMVDMVNLVGVDLNEAASKPYVANLLQYVCGFGPRKAVSVMKVVQANGGRVSSRLELLGDRDRGFLPVVGPMVFINCASFLIIPHDPADRNSDYLDNTRVHPEDYDLGRKMAADALDIDEEDVATMVTEGGAGAVINELINGQDEKVNELSLEDYAEELERNFNQKKRATLETIRAELQHAYEELRNKYQKLSTDEIFTMLTGETKETLAAGMVVPVAIRRVTDRYCAVRLACGIDGNVSLDEMHDGSSHFLPTNFYHVGQTVQARIEKLNEETFFSELSFREDKIRNPLVKRIEFLPDEWDDVREERDKARLSVVNQEQTRTARVIKHPLFKPYNSRQAQEFLAGQSRGDAIIRPSSNGPDHIAVTWKVSDGIYQHIDVLELDKDNEFTVGKTLKVSGKFSYSDLDELIVTHVKAMAKKVDELSHHSKFIQGTKAEAEKWLEKYCEANPKRSMYGFCFDTQRPGYFHLLYKAGANARIGSWPVKIIPHAFQLRDTPFPDVMTLCNGFKTMFHHNSQKSRSRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5GD40</accession>
    <name>D5GD40_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00000956001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430139">
        <property type="protein sequence ID" value="CAZ82433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GD40"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000956001"/>
    <dbReference type="HOGENOM" id="CLU_2470706_0_0_1"/>
    <dbReference type="InParanoid" id="D5GD40"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ82433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="88" mass="9433" checksum="0E3F66FF08560494" modified="2010-06-15" version="1">MSYEFAVSQFSSYTILSILGSLNRLVWVRSLYQTQGHLGRFESRLVLSPPGSVHGGSLAEYGSWHIAPCGVCRACGTVGGLGIAVRTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDI4</accession>
    <name>D5GDI4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 9">Mitogen-activated protein kinase</fullName>
            <ecNumber evidence="1 9">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00001034001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="9">Activated by threonine and tyrosine phosphorylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="1 9"/>
    <dbReference type="EMBL" id="FN430144">
        <property type="protein sequence ID" value="CAZ82577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838386.1">
        <property type="nucleotide sequence ID" value="XM_002838340.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDI4"/>
    <dbReference type="FunCoup" id="D5GDI4">
        <property type="interactions" value="314"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GDI4"/>
    <dbReference type="GeneID" id="9188167"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001034001"/>
    <dbReference type="eggNOG" id="KOG0660">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_181_1_1"/>
    <dbReference type="InParanoid" id="D5GDI4"/>
    <dbReference type="OMA" id="MDIPRPE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000196">
        <property type="term" value="P:cell integrity MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902660">
        <property type="term" value="P:negative regulation of glucose mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902413">
        <property type="term" value="P:negative regulation of mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905665">
        <property type="term" value="P:positive regulation of calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050850">
        <property type="term" value="P:positive regulation of calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032995">
        <property type="term" value="P:regulation of fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07857">
        <property type="entry name" value="STKc_MPK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000013">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000046">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="24"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="387"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="394"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ82577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="427" mass="48455" checksum="C883480D7B0A80D3" modified="2010-06-15" version="1">MDGSPPGRKVFKVFNQDFIVDERYTVTKELGQGAYGIVCAATNAVSLEGVAVKKVTNVFSKKILAKRALREIKLLQHFRGMLITCLYDMDIPKPDAFNEVYLYEELMECDLAAIIRSGQPLTDAHFQSFIYQILCGLKYIHSANVLHRDLKPGNLLVNADCELKICDFGLARGFSVDPEENAGYMTEYVATRWYRAPEIMLSFQSYTKAIDVWSVGCILAELLGGRPFFKGRDYVDQLNQILHYLGTPNEETLNRIGSPRAQEYVRNLPFMPKVPFGRLFPNANPDALDLLNQMLAFDPTSRVSVETALEHSYLQIWHDAQDEPDCPTPFDFSFEVVDEISDMRKMILNEVQVFRAAVRNRRVQHAALDIAALGRKDSSVGIPDHQWAQQQRNDAPRPHDAPRPHDHQMHEANLEQELAGGLDVMHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDJ8</accession>
    <name>D5GDJ8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein BTN</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00001053001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Vacuole membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the battenin family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430147">
        <property type="protein sequence ID" value="CAZ82591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838400.1">
        <property type="nucleotide sequence ID" value="XM_002838354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDJ8"/>
    <dbReference type="FunCoup" id="D5GDJ8">
        <property type="interactions" value="109"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GDJ8"/>
    <dbReference type="GeneID" id="9181596"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001053001"/>
    <dbReference type="eggNOG" id="KOG3880">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029663_1_2_1"/>
    <dbReference type="InParanoid" id="D5GDJ8"/>
    <dbReference type="OMA" id="WLCNWQV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903826">
        <property type="term" value="P:L-arginine transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015819">
        <property type="term" value="P:lysine transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003492">
        <property type="entry name" value="Battenin_disease_Cln3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018460">
        <property type="entry name" value="Battenin_disease_Cln3_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10981">
        <property type="entry name" value="BATTENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10981:SF0">
        <property type="entry name" value="BATTENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02487">
        <property type="entry name" value="CLN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015974">
        <property type="entry name" value="CLN3_BTN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01315">
        <property type="entry name" value="BATTENIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="112"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="136"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="167"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="200"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="301"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="366"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="396"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="437"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ82591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="472" mass="51368" checksum="050AD0CD5313B968" modified="2010-06-15" version="1">MDGSPPANATAPRRQSPLPSIRLPVPGAPSSSLAVYKARFKAAFVGADPRICAAFWLFGLINNVLYVIILSAALDLVGPTLPKAIVLLADVVPSFLTKLIAPYFVHLIPYRMRILILVALSFTGMQLVAWPDSLPVRLFGVVLASVSSGLGELSFLSMTHYYGAFAVPFWSSGTGGAGLIGAGLYVFATSWIGFSVGGSLMVFGLLPTIMLIAFFGILPLAPLRGGNGYEPLRDGDEIIDPDLEGDGATSGLLASSGCPASGHTFPAVNVSYSAGEKTFAGQVDVAWRGFRANFRRTRRLFLPYMLPLLLVYIAEYTINLGVAPTLLFPLASMPFKNYRDAYPMYNTIYQTGVFISRSSTPFFRVHGLYLPALLQVANLVLLVLHSQYNFLPSIWIVFIVVFWEGLLGGLVYVNTFAEITDNTPEDEREFSLGATTVSDSGGICVAGFISLWLEMYLCRWQVDRGRDYCRQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDQ3</accession>
    <name>D5GDQ3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00006224001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430153">
        <property type="protein sequence ID" value="CAZ82646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838455.1">
        <property type="nucleotide sequence ID" value="XM_002838409.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDQ3"/>
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        <property type="interactions" value="12"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GDQ3"/>
    <dbReference type="GeneID" id="9181651"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006224001"/>
    <dbReference type="eggNOG" id="ENOG502S5BU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063296_0_0_1"/>
    <dbReference type="InParanoid" id="D5GDQ3"/>
    <dbReference type="OMA" id="NGHPLCK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DjlA_DnaK_co-chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24074">
        <property type="entry name" value="CO-CHAPERONE PROTEIN DJLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="1">
        <location>
            <begin position="58"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="291" mass="32683" checksum="CD9326E55098E9C4" modified="2010-06-15" version="1">MLASRRSAKRSVNKLSPVGTAISSFLFLAFPEPGVRSYATVNSTNNSKYDWPPNASPTPYQIFNCKQGAPYSKARFYELVKLYHPDHFRHRPGEGPDISEPIRLERFRLIVAANAILSDPVKRANYDYSGAGWNLTSVWETTSTNASQRRWWTEREGHGSFARQHYGKGGPNDPAQNATWEDWERWRQRRAHPGTGGGGEHQGPLYTSNGTFVTVVFILASGGALMNISRANANGHNFLEQREIRHAEISREWARRKREVAVLGSKEERVQTFLKNRDPVRYGIVGPGGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5GE60</accession>
    <name>D5GE60_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00006394001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430164">
        <property type="protein sequence ID" value="CAZ82803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838612.1">
        <property type="nucleotide sequence ID" value="XM_002838566.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GE60"/>
    <dbReference type="STRING" id="656061.D5GE60"/>
    <dbReference type="GeneID" id="9183135"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006394001"/>
    <dbReference type="eggNOG" id="KOG1844">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_338645_0_0_1"/>
    <dbReference type="InParanoid" id="D5GE60"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070210">
        <property type="term" value="C:Rpd3L-Expanded complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034967">
        <property type="term" value="C:Set3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15550">
        <property type="entry name" value="PHD_MLL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46462">
        <property type="entry name" value="UPSET, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46462:SF3">
        <property type="entry name" value="UPSET, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20826">
        <property type="entry name" value="PHD_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zinc finger PHD-type" evidence="6">
        <location>
            <begin position="37"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="98"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="272"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="529"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="608"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="811"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="17"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="98"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="108"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="127"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="274"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="342"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="364"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="383"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="556"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="638"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="692"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="740"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="764"/>
            <end position="781"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ82803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="840" mass="90339" checksum="8AC3467C2B46A8DA" modified="2010-06-15" version="1">MTENPTIPSTPGPSQPPSLANSSTTPEDVADTSGEIDCICEISDDDGFTICCDKCDTWQHLVCVQLNGGNLPKTYYCPRCDPCSLAVLDVQKAKDIQRQRREVEQAKRSHKKKRPTGTSHKKKELANGTNGITSGKVSSSSEKTGVQVSGKSVGGKEMQQPSRKRGHRASAQSSNNAGLSPPITHHAVCNVPNANIPLESPSARNGETESDTDPEKPTRYLGEGFTDLGNSMNKYTGTDVQQLTHKMGDCACLGEKECLVKGVADGETVISKALNGNGNSRKQRRTNASTESTVGSKRPTPDRQSVPLKKEEDEESRSVSPPLSRSKPRSRDLTPSMPQDVAVEKGEMTGREARKFKEVLSRIQKQQQEEQQPQVAKRRKRNSTVSLTPGTSSLHSPGIDMKGWIKVPDSRSGPNHGFVEYSVTDAGVGRRASDSPVSSDERILRRTSSYSPVAQLTQGPNTLKHVSKTVLRPARPQYEDRAIQTDPVEEGIPWWSPAAVKAPPRPPRLPLRKLLMQSLLRDREEAAATVLEDKKRKHDAVSEDPENIPSPKVPKLSNSGSARSTTISPSLRAKGSLHEPLRTATLPTLDIGSLSNQALITPDMPTTMVGASAAKSPSSPRPPGPALADLFSADSDKPSSTQSSPTQEKQPSDKIRVNLPLDKPRVNGYRNSSLHVQLPPGSGAVNGPLTPALQSSLPSAPNSATIQPPSTTFPSYVSPSVSALNSNMVNPSPTRTTKKMSLEDYGKRKHKTEPVEKSDEKLSVKTTNEGPATSTNLSKPSIVSLVEEPTSIADPAPSTLSIVTALPPQAINSSKLAMEPIRIPPAPKDQRTTSLGSSVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEH3</accession>
    <name>D5GEH3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00006484001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430175">
        <property type="protein sequence ID" value="CAZ82916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838725.1">
        <property type="nucleotide sequence ID" value="XM_002838679.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEH3"/>
    <dbReference type="FunCoup" id="D5GEH3">
        <property type="interactions" value="32"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GEH3"/>
    <dbReference type="GeneID" id="9181547"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006484001"/>
    <dbReference type="eggNOG" id="KOG0055">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_9_1"/>
    <dbReference type="InParanoid" id="D5GEH3"/>
    <dbReference type="OMA" id="YPITMSG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03249">
        <property type="entry name" value="ABC_MTABC3_MDL1_MDL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000913">
        <property type="entry name" value="ABC multidrug transporter SitT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221:SF503">
        <property type="entry name" value="MITOCHONDRIAL POTASSIUM CHANNEL ATP-BINDING SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="1"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ82916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="225" mass="24451" checksum="93F107AB10E8301E" modified="2010-06-15" version="1">MEGNIELRDYVALVGPSGCGKSTTISLIERFYNPLAGGVYVDGREISTLNINQYRKHLALVSQEPTLYQGTIRENILLGGDRNDITDDEITAACKSANIYDFIQSLPEGFQTVCGSKGALLSGGQKQRIAIARALIRNPKVLLLDEATSALDSESEKVVQQALDTAAKGRTTVSVAHRLSTIKHADCIYVFDAGRIAESGSHSELMAKRGKYYELVNLQSLGKNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEX7</accession>
    <name>D5GEX7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00006634001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430197">
        <property type="protein sequence ID" value="CAZ83070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838879.1">
        <property type="nucleotide sequence ID" value="XM_002838833.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEX7"/>
    <dbReference type="GeneID" id="9188608"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006634001"/>
    <dbReference type="HOGENOM" id="CLU_2980782_0_0_1"/>
    <dbReference type="InParanoid" id="D5GEX7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="58" mass="6910" checksum="1C2F288D61A46349" modified="2010-06-15" version="1">MTPMIVSFHDIILYFTLFSPVFWISFSSMPRAGQERRIFKFICIVVVLYRICGLRLHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>D5GF04</accession>
    <name>D5GF04_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00006682001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430197">
        <property type="protein sequence ID" value="CAZ83097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838906.1">
        <property type="nucleotide sequence ID" value="XM_002838860.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GF04"/>
    <dbReference type="STRING" id="656061.D5GF04"/>
    <dbReference type="GeneID" id="9188635"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006682001"/>
    <dbReference type="eggNOG" id="KOG2132">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016785_0_1_1"/>
    <dbReference type="InParanoid" id="D5GF04"/>
    <dbReference type="OMA" id="PKLYWHY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.650:FF:000046">
        <property type="entry name" value="JmjC domain-containing protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041667">
        <property type="entry name" value="Cupin_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12461">
        <property type="entry name" value="HYPOXIA-INDUCIBLE FACTOR 1 ALPHA INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12461:SF101">
        <property type="entry name" value="TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13621">
        <property type="entry name" value="Cupin_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="JmjC" evidence="1">
        <location>
            <begin position="169"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="331" mass="36922" checksum="D5DD35D992DB4407" modified="2010-06-15" version="1">MALIMAGAPGNGRREVVEKILAELEGYVGTFLAVHGGEMMEGRRRLTTPPPSVLPQEFRAASTPSPEITHQIPIRPTPPSLGFFQNHLDTANTPLQIQNLLTAWPAVTTNPWSSPSYLLSKTHFGTRLVPIELGKSYTMENWSQKIMPFRDFLKTYILSPEADSSSYPGYLAQHSLFSQIPSLREDILTPDYCYSTPPPAPPGARTHPLEVPIVNAWFGPAGTVSPLHTDPYANILCQVLGRKYVRLYPPSESERLFPRGVEGGGVDMSNTSRVDMDAEGGGVEVEEWERFQEARYLECVLKAGEGLFIPVGWWHYVRSLDTSFSVSFWWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5GF69</accession>
    <name>D5GF69_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">[histone H3]-dimethyl-L-lysine(36) demethylase</fullName>
            <ecNumber evidence="3">1.14.11.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">[Histone-H3]-lysine-36 demethylase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00006749001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N(6),N(6)-dimethyl-L-lysyl(36)-[histone H3] + 2 2-oxoglutarate + 2 O2 = L-lysyl(36)-[histone H3] + 2 formaldehyde + 2 succinate + 2 CO2</text>
            <dbReference type="Rhea" id="RHEA:42032"/>
            <dbReference type="Rhea" id="RHEA-COMP:9785"/>
            <dbReference type="Rhea" id="RHEA-COMP:9787"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:61976"/>
            <dbReference type="EC" id="1.14.11.27"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the JHDM1 histone demethylase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.27" evidence="3"/>
    <dbReference type="EMBL" id="FN430208">
        <property type="protein sequence ID" value="CAZ83162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838971.1">
        <property type="nucleotide sequence ID" value="XM_002838925.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GF69"/>
    <dbReference type="STRING" id="656061.D5GF69"/>
    <dbReference type="GeneID" id="9182346"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006749001"/>
    <dbReference type="eggNOG" id="KOG1633">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_274659_0_0_1"/>
    <dbReference type="InParanoid" id="D5GF69"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140680">
        <property type="term" value="F:histone H3K36me/H3K36me2 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050690">
        <property type="entry name" value="JHDM1_Histone_Demethylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23123">
        <property type="entry name" value="PHD/F-BOX CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="JmjC" evidence="12">
        <location>
            <begin position="223"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="113"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="535"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="745"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="838"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="972"/>
            <end position="1139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="113"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="555"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="579"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="770"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="791"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="838"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="909"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="999"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1012"/>
            <end position="1040"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1048"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="1121"/>
            <end position="1132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ83162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1166" mass="128340" checksum="692086B000671DD6" modified="2010-06-15" version="1">MVNKIDKFYCARCAEVHGPSTMKRKSNRATTAIDYNALHNGSSAPIKNPVDGKLHPYVEIIRNRTFTFADDKLPRMRPEQVTVEFLEDLPNGWNQPFIVPAESNPAPWVTHPKTTILDSDDTTIPQTSTEEHPSRMATPPPPPLATCEGGARRQGADNLDMIIPPGLTVRRVSELIGESARVEMINVLSQATEKDDKWQMDQLVNYFESDVRDVIYNCISCEVSNSELGAMISRPQVVRDLDLADKVWRPDPAPPMGSEAKPRVGKYVLMSVADSFTDFHIDFAGSSVFYHIYEGEKVFLVIPPTEHNLEIYEKWSMDPNMNTTFFPTLTSDPCTLVTLNKGDTMFIPSGWIHAVYTPRDSLVVGGNFLTRNHYAMQMKVQRIEVVTDTKLSQRYPKYTTLMWHLAYNYMKKDPISDEVDEAIGRGRVLKRTRQRSPKTARTYTQQELEGLPALCNFLLRTALVSCGTITTSLVPRRPNLTGKQVEAVKKAIPAPISQEPVEWIKRFGRWCVWKRACQRLVPNGERVPEWALESWWPKDGPKKGPSQAALRRAQRAREEEARKAEPPRRPGLRVRAIRAESAVSSGSEVAQEEGGVPKSRPKKKANGPEAPPGTKKRGRPPNKPQPQPTFDDADHLPVGKRKVTVVEGEDGEAFTLSDGCTYVRKLSNLGPPRAGCQNCRLKKTGCKHKSEIAQLLARLEASDRAAKAARGEREGRRVEPVLAPAPIEDANHKEMTEVQVVVPKPAKEKKAPTPKAKTSIPALKRALPEDANESMEEAEVATQPPPKKKRVEVQVEREESFNPGTQNMAGGRPPGFQGRKPSCDRCKELKARCPHDTWHLAIERQTTRAQKADKPKSHKKGGGRLSLGKVAKSSWDGARQPPAQRRHSSAGNTPTVAHEETPEAEVAEGAEGAEGAEPVLEEIIVVSSKPPPSRLPVPEVRIIKTPTKATTPPAEKDSFELDLSILEEPISYKSRVSHTPEDPSIMAPPLDDLEEPPKPTDPSSPSPPPTTIPATAPSTTVISTSTAASPSLAVPKTLTPPTDPTPPTNGTTAATTTTTTTTSSITSIGKKADYPRLTHRAPKPKVTYTKTTKTVKRKEDEDSISPPPTAKFAKSISPGLTSGGRSGSGGSGKSVSPALAEEDLQVEEIKRMAAKEEFGLRIRRSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFI6</accession>
    <name>D5GFI6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00006916001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430208">
        <property type="protein sequence ID" value="CAZ83279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839088.1">
        <property type="nucleotide sequence ID" value="XM_002839042.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFI6"/>
    <dbReference type="STRING" id="656061.D5GFI6"/>
    <dbReference type="GeneID" id="9188227"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006916001"/>
    <dbReference type="eggNOG" id="ENOG502QUH1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039854_1_0_1"/>
    <dbReference type="InParanoid" id="D5GFI6"/>
    <dbReference type="OMA" id="FFNAWMS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006740">
        <property type="term" value="P:NADPH regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000030">
        <property type="entry name" value="NAD binding Rossmann fold oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000778">
        <property type="entry name" value="NAD binding Rossmann fold oxidoreductase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42840:SF6">
        <property type="entry name" value="BINDING ROSSMANN FOLD OXIDOREDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G11930)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42840">
        <property type="entry name" value="NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase N-terminal" evidence="1">
        <location>
            <begin position="99"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="413" mass="45593" checksum="4010B2F7DE0663A2" modified="2010-06-15" version="1">MGLVNVLMVGTGEYTTGWVGTGGSTSDKKVGVVGLTLFDLRRRGKVGELSMVGVNGKKYPDIRNHLEKNIKSVYNNLDVSFNSFPADDAVDPDSYKTAIDALSPGDAITIFTPDTTHFPIALYAIRKGIHVLITKPATKTLEDHLALIEEAEKAKVFVYVEHHKRFDPVYSDARAKAKNLGDFNYFYSYMSQPKSQLETFRAWAGKESDISYYLNSHHIDINESMVPGYRPFKVTASGSTGCATGLGCVGGTEDTITLLTDWVKKDDPSKTATGVYIACWTAPQKAGVHSNQYFHYMASKGEIRANQSKRSYDVTDDQDGLTWYNPFYMRYAPDEEGNFGGQTGYGYISFEKFVDACAALNEGRITLAELDRRGLSTLRNTVVTGAILHAGRISLDEKRSVGIEEVEGTLRLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFU2</accession>
    <name>D5GFU2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00007080001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430220">
        <property type="protein sequence ID" value="CAZ83385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839194.1">
        <property type="nucleotide sequence ID" value="XM_002839148.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFU2"/>
    <dbReference type="GeneID" id="9187296"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007080001"/>
    <dbReference type="HOGENOM" id="CLU_2172909_0_0_1"/>
    <dbReference type="InParanoid" id="D5GFU2"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ83385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="110" mass="12441" checksum="7D34DD274E5C2CAD" modified="2010-06-15" version="1">MEGEALSFGVGMNCLPVPGVELRNVRGIVEAILMIAVKIDGSGAPTHDLCYAPRRERKRKTQDTPHFFLLYSIIYPVPKLFSQLRYIHTPPNVLVLTFPTPVYFLLSKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFV9</accession>
    <name>D5GFV9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Folylpolyglutamate synthase</fullName>
            <ecNumber evidence="17">6.3.2.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Folylpoly-gamma-glutamate synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Tetrahydrofolylpolyglutamate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">GSTUM_00007110001</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Catalyzes conversion of folates to polyglutamate derivatives allowing concentration of folate compounds in the cell and the intracellular retention of these cofactors, which are important substrates for most of the folate-dependent enzymes that are involved in one-carbon transfer reactions involved in purine, pyrimidine and amino acid synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 17">
            <text>(6S)-5,6,7,8-tetrahydrofolyl-(gamma-L-Glu)(n) + L-glutamate + ATP = (6S)-5,6,7,8-tetrahydrofolyl-(gamma-L-Glu)(n+1) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10580"/>
            <dbReference type="Rhea" id="RHEA-COMP:14738"/>
            <dbReference type="Rhea" id="RHEA-COMP:14740"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:141005"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>a monovalent cation</name>
            <dbReference type="ChEBI" id="CHEBI:60242"/>
        </cofactor>
        <text evidence="17">A monovalent cation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 17">Cofactor biosynthesis; tetrahydrofolylpolyglutamate biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 17">Belongs to the folylpolyglutamate synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.17" evidence="17"/>
    <dbReference type="EMBL" id="FN430220">
        <property type="protein sequence ID" value="CAZ83402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839211.1">
        <property type="nucleotide sequence ID" value="XM_002839165.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFV9"/>
    <dbReference type="FunCoup" id="D5GFV9">
        <property type="interactions" value="635"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GFV9"/>
    <dbReference type="GeneID" id="9181747"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007110001"/>
    <dbReference type="eggNOG" id="KOG2525">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015869_0_1_1"/>
    <dbReference type="InParanoid" id="D5GFV9"/>
    <dbReference type="OMA" id="ESLDCCM"/>
    <dbReference type="UniPathway" id="UPA00850"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004326">
        <property type="term" value="F:tetrahydrofolylpolyglutamate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1190.10:FF:000009">
        <property type="entry name" value="Folylpolyglutamate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="entry name" value="Mur ligase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="entry name" value="Mur-like, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001645">
        <property type="entry name" value="Folylpolyglutamate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018109">
        <property type="entry name" value="Folylpolyglutamate_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023600">
        <property type="entry name" value="Folylpolyglutamate_synth_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01499">
        <property type="entry name" value="folC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136:SF5">
        <property type="entry name" value="FOLYLPOLYGLUTAMATE SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136">
        <property type="entry name" value="FOLYLPOLYGLUTAMATE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038895">
        <property type="entry name" value="FPGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="MurD-like peptide ligases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="MurD-like peptide ligases, peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01011">
        <property type="entry name" value="FOLYLPOLYGLU_SYNT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01012">
        <property type="entry name" value="FOLYLPOLYGLU_SYNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 17" id="KW-0436">Ligase</keyword>
    <keyword evidence="13 19" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="10 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 17" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="19">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="343"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="357"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR038895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR038895-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR038895-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CAZ83402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="521" mass="56995" checksum="402149C9D2F7ABD0" modified="2010-06-15" version="1">MRFFQLRSTLLKKRQYTTTFYRTMASTQFPKTYEGAVAALNTLQSNFALLDEIRKSGMKMNSSAMAEMVESVRQLGYKPSDFDRLNIIHVAGTKGKGSTCAFTASILAQYKPLGGAAAKLGLYTSPHLRAVRERIQIDGAPLEEELFAKYFFEVWDRLEASAVAEGRDPTIKPVYFRFLTLVAFHTYLCENVTTAIFEVGIGGEHDSTNVIQKPTVVGISSLGIDHTAVLGNTIEEIAWHKAGIIKSSARTFTVPQRSAAMAVIQKRADEKGSKVEVVHVHPQIWSREVKLGLPAQFMNGNASLAVALAAEHLTLLGMNPGIINGKLPERFVKGLAEVVWPGRCQVIKTGAMEWCIDGAHTVESLEVCGKWFGARADGIEKRVLIFNQQKRDSDALITALANSLKEILGVNSRIFDEVIFCTNITYKGRGQKADLMNAGTDPAALENLSVQKKLAQAWNNVVPATKASHVVPTIGEAIGIVTALSFKAPVQVLVTGSIHLVGAFLEVLGEKYPNQVHPVRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGC0</accession>
    <name>D5GGC0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATP synthase subunit 9, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Lipid-binding protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">GSTUM_00002010001</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. A homomeric c-ring of probably 10 subunits is part of the complex rotary element.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the ATPase C chain family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430254">
        <property type="protein sequence ID" value="CAZ83563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839372.1">
        <property type="nucleotide sequence ID" value="XM_002839326.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGC0"/>
    <dbReference type="FunCoup" id="D5GGC0">
        <property type="interactions" value="682"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GGC0"/>
    <dbReference type="GeneID" id="9183161"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002010001"/>
    <dbReference type="eggNOG" id="KOG3025">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148047_4_1_1"/>
    <dbReference type="InParanoid" id="D5GGC0"/>
    <dbReference type="OMA" id="YIFGKMI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033177">
        <property type="term" value="C:proton-transporting two-sector ATPase complex, proton-transporting domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18182">
        <property type="entry name" value="ATP-synt_Fo_c_ATP5G3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.20.10:FF:000009">
        <property type="entry name" value="ATP synthase subunit 9, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.20.10">
        <property type="entry name" value="F1F0 ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01396">
        <property type="entry name" value="ATP_synth_c_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000454">
        <property type="entry name" value="ATP_synth_F0_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020537">
        <property type="entry name" value="ATP_synth_F0_csu_DDCD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038662">
        <property type="entry name" value="ATP_synth_F0_csu_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002379">
        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035921">
        <property type="entry name" value="F/V-ATP_Csub_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10031">
        <property type="entry name" value="ATP SYNTHASE LIPID-BINDING PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10031:SF0">
        <property type="entry name" value="ATPASE PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00137">
        <property type="entry name" value="ATP-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00124">
        <property type="entry name" value="ATPASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81333">
        <property type="entry name" value="F1F0 ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00605">
        <property type="entry name" value="ATPASE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0138">CF(0)</keyword>
    <keyword evidence="9 16" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="11 16" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 16" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="14 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6 16" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="48"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="17">
        <location>
            <begin position="8"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00030961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU004221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAZ83563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="74" mass="7535" checksum="5976A3E8D7F18236" modified="2010-06-15" version="1">MIQASKIIGTGLATTGLIGAGVGIGVVFGALILGVARNPSLRAQLFSYAILGFAFCEATGLFALMIAFLLLYVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5GH09</accession>
    <name>D5GH09_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00007647001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the WD repeat cdt2 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430297">
        <property type="protein sequence ID" value="CAZ83802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839611.1">
        <property type="nucleotide sequence ID" value="XM_002839565.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GH09"/>
    <dbReference type="STRING" id="656061.D5GH09"/>
    <dbReference type="GeneID" id="9185056"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007647001"/>
    <dbReference type="eggNOG" id="KOG0321">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_624366_0_0_1"/>
    <dbReference type="InParanoid" id="D5GH09"/>
    <dbReference type="OMA" id="CIFATAP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051865">
        <property type="entry name" value="WD-repeat_CDT2_adapter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22852:SF0">
        <property type="entry name" value="DENTICLELESS PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22852">
        <property type="entry name" value="LETHAL 2 DENTICLELESS PROTEIN RETINOIC ACID-REGULATED NUCLEAR MATRIX-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="2 6" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="45"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="330"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="330"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="351"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="374"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ83802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="395" mass="43966" checksum="12942F95385BF15E" modified="2010-06-15" version="1">MVSPAHNIPIFFNRQTNERYAQATACGDQKIRVMDITKQTILHVLDGHVSSVKQVAFEPTCPSITASCSRDGTAMIWDLRANIQSRSDNNEFVTHRPVEVIRGAHAPLTAPKGRAPRSIVSVTALTWGIPNTFLTACESRAELRCWDRRSIKKDRRGNSQPCDVSAEPEHHQVRPFGINSISISPDRSMAYTLCKDSIVYAWGTRLLEHGPLHAYHHPELDASSFYVKSDISHDGEYLATGSSSGSACIFATAPEYLDKKLYIQKTDFPEGSRVQAVSKKMSVGTGVVLVRAHNREVTDVSWTNKGDLVTISDDYHARCWRSDDKGVNAENMRENGEENGRKWGWGWAQLEGEKNEGKDAEPSDERSIPPVNDPTDEPDFQEGEDGGTADHYPQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5GH35</accession>
    <name>D5GH35_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00007686001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430297">
        <property type="protein sequence ID" value="CAZ83828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839637.1">
        <property type="nucleotide sequence ID" value="XM_002839591.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GH35"/>
    <dbReference type="FunCoup" id="D5GH35">
        <property type="interactions" value="60"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GH35"/>
    <dbReference type="GeneID" id="9185082"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007686001"/>
    <dbReference type="eggNOG" id="KOG3780">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008578_2_1_1"/>
    <dbReference type="InParanoid" id="D5GH35"/>
    <dbReference type="OMA" id="FHILSCK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070086">
        <property type="term" value="P:ubiquitin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050357">
        <property type="entry name" value="Arrestin_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188:SF174">
        <property type="entry name" value="ARRESTIN-RELATED TRAFFICKING ADAPTER 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01017">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="2">
        <location>
            <begin position="427"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="728"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ83828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="771" mass="83581" checksum="A94D48014645A27D" modified="2010-06-15" version="1">MMTSAAPTPAVHGPAPPNYSHHRPAATTTTSTQHSNRRNRPRNTNCRRNRLSTPVIPSLQSSTYSSAESIARRIQRTPSAVAKKLFASAVSSPSTFCSRRSPPQPPASPPMPTGMGRAIGGGNSGIPSHHVKNSSAPVRPTSAILTPRDCEGGHSSGLRDANGDIAPLTSEKPQASTSGGVVTMTIQLAEPMLFLQGFDQSDHSNRAPAMLRGSLLLRISKPTKIKAVSLTFRGRARTEWPEGIPPKKSEYFEEKEIMQHTWPFFNAQFATAELGHGADSCRLYKSSSDCTSSITSAIGHLARSASPIGISPALGSCSTDRRLSLQLTQSRSFSKGELPNEVSVAQRGYRIFQPGDYVYNFELPIESCLPETIDVDLGAVKYELEGTIVRPGAFRPNLVGKRDVILIRCPAETSLECSEPIAISRTWEDQLHYDIVISGKSFPLGSTIPIAFKLTPLAKVQCHRIKIFITENIGYSCRNKKSNRLDPTRKVQLFEKRADGPSISTFAGSSARILAGGGFNGEQVAGGRNLDGGNGSDNLLGDLTGKDNIGPTEMELAVQLPGCNAKEKDRIHFDTTYQNIQIVMRLSKADPNEPGKRRHFEISIDSPLHILSCRAAGANTALPAYNSPNPGIASSGTQCSCPSLTRKNSPRGGTPTLNVDTQVGHLSSGQPEPPNEAARPLHLIRYPSHNPPPFDYDVAPPPLITPPPKYELADYFQRLKDETARDDDIDDEHVNGDSRRLLPPLTPGGRIARSMDERRTWEPLGISNQAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>D5GH81</accession>
    <name>D5GH81_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00007788001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430308">
        <property type="protein sequence ID" value="CAZ83906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839715.1">
        <property type="nucleotide sequence ID" value="XM_002839669.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GH81"/>
    <dbReference type="STRING" id="656061.D5GH81"/>
    <dbReference type="GeneID" id="9182611"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007788001"/>
    <dbReference type="eggNOG" id="ENOG502QSII">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020417_0_0_1"/>
    <dbReference type="InParanoid" id="D5GH81"/>
    <dbReference type="OMA" id="GVMMKRV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042149">
        <property type="term" value="P:cellular response to glucose starvation"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="AreA_GATA"/>
    </dbReference>
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        <property type="entry name" value="Glucose_repression_reg"/>
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        <property type="entry name" value="PROTEIN MTL1-RELATED"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="GATA_AreA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitrogen regulatory protein areA GATA-like" evidence="2">
        <location>
            <begin position="177"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="369"/>
            <end position="405"/>
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    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="370"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ83906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="575" mass="63599" checksum="E4B2761759398B2E" modified="2010-06-15" version="1">MTEIMAAPIHLDVCYQPGSSPRCRSPQSAYAGPTGSYVDSLSNSEYDPYDSHKSSPPPSSISSSSPFSSIASQSSVPSSVASSVCLDTIPIDNDDQFLFPVYDQSAVSYCSDEYDYTSEDYNTPVPGTSPRSPSPPTIPEIPEIIFVPADDSVVRHEPSRHVDYLSHDWNEEDIWASWRYMVGKRKVYNNAARLENASWRTWAKAKFKLKTVSPETLNWLKDCDVTWLYGPLSTSNQKASLMVSPSSPTVSPTEPRIPSSGSFYVKKPILKKRSMSEVMLQKSLSSSSLLKQAAAAIESQQVEMDCKTSRPAMVARANSDFAALSYASKTTTAINTSELPSALSTGCPSPSYTKHIHFNDQVQQCIAVDKDDEDDEEDEDEEENEVFQVENLEDSSSDDDDGLLMMPRPKVAVKSTTRTNSGEHQTIAMLPSTTLKYVEEPVVKKNDTFNNIGTFISSFATPAPTQPQDLDQSSLVGTKSYILEDDDDDMAELDWEPSGGAFRSRRDSVAVAHDRFSRADVDMGNAHRDDFELPKYTYEEDEDDEVAAGLFGRAVDAVNTARDIAHVLWNVGWRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHG9</accession>
    <name>D5GHG9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Vacuolar import and degradation protein 21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00007867001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of selected genes principally by acetylation of nucleosomal histone H4 and H2A. The NuA4 complex is also involved in DNA repair.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EAF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430318">
        <property type="protein sequence ID" value="CAZ83962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002839725.1"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GHG9"/>
    <dbReference type="GeneID" id="9183607"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007867001"/>
    <dbReference type="eggNOG" id="KOG0391">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_240364_0_0_1"/>
    <dbReference type="InParanoid" id="D5GHG9"/>
    <dbReference type="OMA" id="PRRRKCH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
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        <property type="entry name" value="HSA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46459:SF1">
        <property type="entry name" value="E1A-BINDING PROTEIN P400"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46459">
        <property type="entry name" value="E1A-BINDING PROTEIN P400-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07529">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00573">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51204">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HSA" evidence="11">
        <location>
            <begin position="916"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="10">
        <location>
            <begin position="1211"/>
            <end position="1263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="161"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="241"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="487"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="521"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="689"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1111"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1551"/>
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        </location>
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            <begin position="1664"/>
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            <begin position="174"/>
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        </location>
    </feature>
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            <begin position="189"/>
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        </location>
    </feature>
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        <location>
            <begin position="264"/>
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        </location>
    </feature>
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        <location>
            <begin position="275"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="310"/>
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    </feature>
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        <location>
            <begin position="342"/>
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        <location>
            <begin position="398"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="603"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="689"/>
            <end position="698"/>
        </location>
    </feature>
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        <location>
            <begin position="711"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="747"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="1128"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="1151"/>
            <end position="1160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1551"/>
            <end position="1563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1689"/>
            <end position="1718"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ83962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1718" mass="188151" checksum="97117F9C4475972D" modified="2010-06-15" version="1">MVEKISIQSLRNNAVSVRLEERKNLIVSRKRKLQEVYAACRHIDRTKPFPNADIVKTWGTIERYEGANMEDGEKRFLEENDIERGRFFQESTLPPHSSYLPEPGGFQRALATQLTPHHAAKTISRHATPLSTSVFEKPPQVLEHPTVSREAELLAEKGARHSFDARGGMEDQSPAKSVITNGKRSSEAPTEDGSVDSEATEEDLGSLSHVVEPSERVKPRKAAPVINIGGVPVVQEAVTKAPDDHERSLGVRPPPIPSELVNATRGSATQTAAVQDEKTEERDARAEEARSTESAAVKSFGDSAREVPDSFESSQVSSPSEPPSPAPSKGTVLELREEPRLPVTSPQIQQTPPQPPLVIITNRENHTSPVPPSKPLSGHASHSDVYSPASSIDDDNRSTPGNILSSATSPDANHMLGPDPRSEESNGQVKEVVAESGPYGVMTMPAKEAEEEELAGAFVEKIVPCGAQCEKPLPSVVAAKGFVADEEETKSQLSTIPPLEFTNGGEPDLAAVEAGAAITISKADDLSARTKKSASPDPSAQLRLENSLARDDVADPDSLASEGPISSQKLAGVGETHDEDLEDRVQLVGSREPEPRAAGPMRELAEDVKEVKDVSETMEIDIPDRVGDMGATSHDTVMSGVDQPVAHRTTPGTHAGDISVGTISSEQHSLEASTLQEIDDSKMEIDHTANERHDEAEPPSKVPTPDVSTFVGATTSPGSPASSMFGPGSGEEAPSTDRMAVGRAESDSSLIPSTSPVKQPLRRPETRKKQAKLSKVVISSTHHALAALEEEAKRLHEDQIAQGPIVVARRRDQQAASAAARRSAVFQPGSRIEAITRYGIAEPPSEEKRRVKNADMFDVVYTAKASGTLISDLLQRSSKTLTTGDHQVHYFETQAVRIENRIIELQDQGLWSLRQIPRVVEPRRRKCHWDYLLEEAKWLRDDFKGERKWKIAMARHYAKEAVRWHQAGEERCQLQIDRKLLGVVPKGKREERRRAARIEMQDSDMNQPTPDLVDGCGSPESDDDFPMDDRHHDTDQGNFAHIYEPPATLFTLSPEETVFAMPASKAADDILSQLPLYGPPEPSAGRQEEVEEEWTKLPVVAISKYASGKLVIQEEQGPPSKKSRYEYEENQDPYADDSDEDEKLGGGRLIGRGKRDKLRKPSFLPPEQTNVALFKPEYKPILQRVHNTHVPRLPSDFPPPPFFENRHPSLWLASEDEMLKTLVREYHYNWGLVSQCLTLPGDWHSGSERRSPWECFERWIALEPIPPEFSKSPHFKPIQQRLDVAARVNGLYGNVPGSAGGNGSGGVKPRRGTAPIRVEKRRNTRNFTLIESMRKLAKKRETSVNKQQTNKSTMISLLLTSYLDQVVAAMKRASESQASTANPLRTPQYFSAMKFEREQKHLEQKNIYSQHMQMQQTRTGIPPQARVPHPGQHHALANGVPIPVQQRVGVATPQGPNGVLQVSRAAPIQNGTNGVPLRSLPAQLSQGQTQRYTPEQLAHLRRIQQQHTVAAAAASQGYPTSNALGTQASQPSAQSMQTILQQTQQAALGQNQQVNVNQQAPQQKRIIPPPIDSQASGSSPRMPPTPTTATSTQLKPAQILIQEMAAIAQYQNPNLPQERAREATQPTLASYHAQLSQNIQAQARSPVRPNPARVAVVNTGPMIGVNGPVNPAVDPPAVGAGSPRVSNGQIPTQLPQPQQPQANQPQPQPQSVSQQQPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHH5</accession>
    <name>D5GHH5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00007879001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430318">
        <property type="protein sequence ID" value="CAZ83968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839777.1">
        <property type="nucleotide sequence ID" value="XM_002839731.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHH5"/>
    <dbReference type="GeneID" id="9183613"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007879001"/>
    <dbReference type="eggNOG" id="ENOG502SCCP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_413995_0_0_1"/>
    <dbReference type="InParanoid" id="D5GHH5"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ83968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="663" mass="75500" checksum="FECE2AC2D5BDF4C3" modified="2010-06-15" version="1">MNLPITDLQTIRRYSIRSHRRDYRQTFTSTTSLEVKDVQEKPDVLNLMLKSAATGEAPDYRSLFLTLLDLLASSRAAQKLRYPDTSNKSTQQRILLFTEEWPKQLDMNRNVLGLCKDVVVKALSPDADVPDEDLWRSVYSLLRTLHFSVSDLNIIEENPLQDFLITYRQQFGTNYRQFYLRTAGGASGLQGSSETLDLLLKEAIQDNQFNNMFSIFLQQIALTPASAELRHPIGTDTSVRQRILLFAEEWPHQLAPNSDTPDLYKDALLKALTPGLDKEFWYCMYKLLDSFYFSKIELEKIEGNPLQDFRSKYRLFFLENTETTIDRVLWDSEVLKEMLKNATVHESSTGRKLFRVFLSRLAATEASQELRHPLGSGTRVKQRIMLLANEWPNQLVLNNKALDICADVLLKTLIPETDVSDRELWYSIYHLLNALHFSELDLQVIRETPLRVFRRSYRLFFVKKSRFTMDQVLGSPATLRFLLRDSSSRATGPGSRELLHVFLCQLALTDVSGELKHPIASDTTVGKRILLFAEALPKTLALGGDVLRHCVDVITKALSPDDEVSDTEFWYSMYHLLNAFPAEESPRANRNIATMAKDYAVKYAKEHPYLLALNVGLTVVGFAIPPLLGALGFTRAGVSAGLVPRAPSLTFDGCRIFLFISIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GI05</accession>
    <name>D5GI05_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00008217001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430322">
        <property type="protein sequence ID" value="CAZ84148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839957.1">
        <property type="nucleotide sequence ID" value="XM_002839911.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GI05"/>
    <dbReference type="GeneID" id="9184233"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008217001"/>
    <dbReference type="HOGENOM" id="CLU_2225120_0_0_1"/>
    <dbReference type="InParanoid" id="D5GI05"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ84148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="106" mass="11992" checksum="6051DBB80F92DA15" modified="2010-06-15" version="1">MANSQYGSMIPNSHIALKFSSGQESPQPHQPSQVLYCMIQVQYKYKYSAGTDQPRPKERKEIPVNPLLPSSPLYQKLRKVLVTTVLHSTAHYSCAEKEGRTKARSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>D5GI18</accession>
    <name>D5GI18_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00008232001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430322">
        <property type="protein sequence ID" value="CAZ84161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839970.1">
        <property type="nucleotide sequence ID" value="XM_002839924.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GI18"/>
    <dbReference type="STRING" id="656061.D5GI18"/>
    <dbReference type="GeneID" id="9183494"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008232001"/>
    <dbReference type="eggNOG" id="ENOG502TFR4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_541006_0_0_1"/>
    <dbReference type="InParanoid" id="D5GI18"/>
    <dbReference type="OMA" id="WETEQSI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="478"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="198"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="478"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="493"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ84161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="504" mass="58015" checksum="FF89BC19AC57E373" modified="2010-06-15" version="1">MTSASHLELQDRIQFTTSLSDLVVESQQLRTAILSSLVTNKLSHPIAKFLSAHDKSPTYTCQDWEVCPAGLLQLQNKLAVALCTLVLRAGIYSQKDDTIFLDPSLATSLLEKQLDLSKIKNTCSAHYSIRSNPNSPSIVSIFETSATPDIKTNSRDWRMHLREAMLRDAGRQNDVIIAEVGMICRDLEIRCDGVEKPLRDEEERSEKLRQEIKDLEARLEEETEERSSLNETLEHEREEFKYKLDGENEKARELFSRIGDLERELQTARDEASRVRSESQEAFEKLDAQHKAEMVRIGIESQEVLEGLRSSHEAELRQVHHDSQEALEKLSASHKAERKAAQEVADELALGHIEVLNQRQEAIEELETQNRSLEQQSDRLREALEEQRKENHAADEKVIRMDERIRGMDRELSELSLEMECQKTALDRKDNEIETLKRQLQAAKSDAEDIRRVMDAEVARLQENLKHQKDLAQELERKLEGTEKEARERIDNLETQNNSKVRLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIQ0</accession>
    <name>D5GIQ0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008603001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430328">
        <property type="protein sequence ID" value="CAZ84393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840202.1">
        <property type="nucleotide sequence ID" value="XM_002840156.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIQ0"/>
    <dbReference type="FunCoup" id="D5GIQ0">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="GeneID" id="9183327"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008603001"/>
    <dbReference type="eggNOG" id="ENOG502S6Z8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2110722_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIQ0"/>
    <dbReference type="OMA" id="MNASAYF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013726">
        <property type="entry name" value="Mitofissin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28075">
        <property type="entry name" value="CHROMOSOME 16, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28075:SF1">
        <property type="entry name" value="DUF1748-DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08520">
        <property type="entry name" value="Mitofissin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="115" mass="13036" checksum="D2517A08D35EFD38" modified="2010-06-15" version="1">MQLSQDCEFIQLLVYTIALFPLVPKSAAILLFLLPCFISLPLNMLGKIAHYSFDAILFSAFLAGVKRSTGLTFQTEKIESKDVRSAIDKYLNIGEWFMDQSIAAMGSSNYFERRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIR0</accession>
    <name>D5GIR0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GTP cyclohydrolase II</fullName>
            <ecNumber evidence="3">3.5.4.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00008617001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>GTP + 4 H2O = 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + formate + 2 phosphate + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:23704"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58614"/>
            <dbReference type="EC" id="3.5.4.25"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; riboflavin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GTP cyclohydrolase II family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.25" evidence="3"/>
    <dbReference type="EMBL" id="FN430328">
        <property type="protein sequence ID" value="CAZ84403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840212.1">
        <property type="nucleotide sequence ID" value="XM_002840166.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIR0"/>
    <dbReference type="FunCoup" id="D5GIR0">
        <property type="interactions" value="126"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GIR0"/>
    <dbReference type="GeneID" id="9185621"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008617001"/>
    <dbReference type="eggNOG" id="KOG1284">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020273_2_4_1"/>
    <dbReference type="InParanoid" id="D5GIR0"/>
    <dbReference type="OMA" id="RLPNVEC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003935">
        <property type="term" value="F:GTP cyclohydrolase II activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00641">
        <property type="entry name" value="GTP_cyclohydro2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10990">
        <property type="entry name" value="GTP cyclohydrolase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032677">
        <property type="entry name" value="GTP_cyclohydro_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000926">
        <property type="entry name" value="RibA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036144">
        <property type="entry name" value="RibA-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001591">
        <property type="entry name" value="PRK00393.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00505">
        <property type="entry name" value="ribA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21327">
        <property type="entry name" value="GTP CYCLOHYDROLASE II-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21327:SF29">
        <property type="entry name" value="GTP CYCLOHYDROLASE-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00925">
        <property type="entry name" value="GTP_cyclohydro2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142695">
        <property type="entry name" value="RibA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0686">Riboflavin biosynthesis</keyword>
    <feature type="domain" description="GTP cyclohydrolase II" evidence="10">
        <location>
            <begin position="243"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="195"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="209"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ84403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="411" mass="43977" checksum="4B14F21E8446AC54" modified="2010-06-15" version="1">MSTAETTRSLDTPPSSDLVSGESPREGGSDSMSIYSPKPKVIGNGYYLGSNSGIDPSMETIAPPAHASPTLLTVNPGSRSDSAMLLGEEGQIPPSILSPAFTPPTTPSTPLMGLAENTSPPMLMEELPKVNCMVRARIPTTTGTEIFLHLYQNNIDNKEHLAIVFGNRIRSRSLDAPREGETEMDRMIRGAYTGRLFPGRTSSHADDSPMSSQVPSRAASSTSLSNMAPTTQLTTPGSGTRTAPLVRIHSECYTGEIAWSARCDCGEQLDEAARLMSLDPAGGVVVYLRQEGRGIGLGEKLKAYNLQDLGNDTVQANLILRHPADARSYGLATAILMDLESVNIRLLTNNPDKIAAVEGPGKEVRVVERVPMIPLAWQNLKGGVTGKEVGDYLRTKITQMGHLLDLPANGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIS3</accession>
    <name>D5GIS3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00008644001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430328">
        <property type="protein sequence ID" value="CAZ84416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840225.1">
        <property type="nucleotide sequence ID" value="XM_002840179.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIS3"/>
    <dbReference type="STRING" id="656061.D5GIS3"/>
    <dbReference type="GeneID" id="9185634"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008644001"/>
    <dbReference type="eggNOG" id="KOG0527">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010453_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIS3"/>
    <dbReference type="OMA" id="WAWCANI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01389">
        <property type="entry name" value="HMG-box_ROX1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000041">
        <property type="entry name" value="HMG box family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050140">
        <property type="entry name" value="SRY-related_HMG-box_TF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10270:SF161">
        <property type="entry name" value="SEX-DETERMINING REGION Y PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10270">
        <property type="entry name" value="SOX TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HMG box" evidence="6">
        <location>
            <begin position="174"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="4">
        <location>
            <begin position="174"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="236"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="283"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="427"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="253"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="285"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="305"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="435"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ84416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="705" mass="75604" checksum="AFD2B56433E7BA37" modified="2010-06-15" version="1">MAETVVEAPNVDEEAGGLRVLLEEDLSDSRSLMDNTPSLMAAGSTSPQVDVNAMVVAQQHAAAHQARAAAALEQADAAEERNHRQNHPQLQHVINNAHSQAPEKVPVSSTPPPPSTVTQDLPQQTVPHAAPIKIPSRSTVEEENGMPGTPGTSDLALLVVSPSNICLCQQPARIPRPRNAFILFRQHNQAAVVAKHPGKPNPEISKIIGEMWRESSQEAKNLWQRHADEEKKKHFARYPEYRYQPRRNGKKNAAAAAGASGSASGGAISPPLETAICSTCGGRTGSLSTPQTPATPGSAVPKTPGLSQLPHSARTPTSVPPPSKRRRAGTEDDAPRCSTGSPAGPVGEQAGIEALLQLGNLGDDENIVATELQASTKRQKMNGGSPTTDALTTSPLQHSLYPGHFPLQSHNPNIDPFLQQNSFEQSMPSLDADGSLTSGPHQISNFSPELPEPHIPKPISPMQKIRAFNCICKPLPTGAGFSRLPVIAIEGDDIEAVSELFHAVAKLISNSDPIDIIDEPDISVSEQPHKLEESTEISDGLLMPAGDGNDLDSAGVLKDAEDDLEAAKYLDHVASWRRRCLRIKNAVMHSNGASQRMVLVNRYMVSRSNSAYAKLSSDGLSALQHWQWCATVWRGCIGPDMTIYVVNVPNGSIGGDAVEAKEGGKVVFVKKEVGELGRKAWEEKVIRRLAFEVGEIVRSLKGFMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJF5</accession>
    <name>D5GJF5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">cellulase</fullName>
            <ecNumber evidence="3">3.2.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00008973001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endohydrolysis of (1-&gt;4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.4" evidence="3"/>
    <dbReference type="EMBL" id="FN430330">
        <property type="protein sequence ID" value="CAZ84648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840457.1">
        <property type="nucleotide sequence ID" value="XM_002840411.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJF5"/>
    <dbReference type="STRING" id="656061.D5GJF5"/>
    <dbReference type="GeneID" id="9182649"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008973001"/>
    <dbReference type="eggNOG" id="ENOG502QXN4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029718_0_2_1"/>
    <dbReference type="InParanoid" id="D5GJF5"/>
    <dbReference type="OMA" id="GDYFQSI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008810">
        <property type="term" value="F:cellulase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009251">
        <property type="term" value="P:glucan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34142">
        <property type="entry name" value="ENDO-BETA-1,4-GLUCANASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34142:SF1">
        <property type="entry name" value="GLYCOSIDE HYDROLASE FAMILY 5 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="7">
        <location>
            <begin position="45"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ84648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="342" mass="37053" checksum="74C6CD8628193ABE" modified="2010-06-15" version="1">MPDILLLQRRRAIVRESDLHLGDTGSACSGATKFKYFGGDFNQAGAEFAGSVIPGQLGTHYTWPKTSSIDYFLDLGMNTFRIPFSMERLVPPAQGLAGTMDATYLAALKNIATYITGKGAHAIIDPHNYGRYNGAIITDTAAFGKWCKNIASEFKSDYNIIFDTNNEYHDMDQELVFNLNQACINGIRAAGATSQLILVEGNSWTGAWSWVSSGNAASMIDLEDPNDNIAFEMHQYLDGDSSGTSQNCVSSTIGAERLTATTNWLKHNGKKGFLGEMGAGSNDACIAAVYGALCHMQQAGGVWIGALWWAAGPWWGNYFQSIEPPDGASISRILPEALMPFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJI9</accession>
    <name>D5GJI9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Glucose-6-phosphate isomerase</fullName>
            <ecNumber evidence="3 10">5.3.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00009036001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">In the cytoplasm, catalyzes the conversion of glucose-6-phosphate to fructose-6-phosphate, the second step in glycolysis, and the reverse reaction during gluconeogenesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>alpha-D-glucose 6-phosphate = beta-D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:11816"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:58225"/>
            <dbReference type="EC" id="5.3.1.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the GPI family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.9" evidence="3 10"/>
    <dbReference type="EMBL" id="FN430332">
        <property type="protein sequence ID" value="CAZ84682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840491.1">
        <property type="nucleotide sequence ID" value="XM_002840445.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJI9"/>
    <dbReference type="FunCoup" id="D5GJI9">
        <property type="interactions" value="998"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJI9"/>
    <dbReference type="GeneID" id="9182683"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009036001"/>
    <dbReference type="eggNOG" id="KOG2446">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017947_3_1_1"/>
    <dbReference type="InParanoid" id="D5GJI9"/>
    <dbReference type="OMA" id="DWYRQLW"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00181"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047938">
        <property type="term" value="F:glucose-6-phosphate 1-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004347">
        <property type="term" value="F:glucose-6-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048029">
        <property type="term" value="F:monosaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061621">
        <property type="term" value="P:canonical glycolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051156">
        <property type="term" value="P:glucose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05015">
        <property type="entry name" value="SIS_PGI_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05016">
        <property type="entry name" value="SIS_PGI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1390.10:FF:000001">
        <property type="entry name" value="Glucose-6-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000004">
        <property type="entry name" value="Glucose-6-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00473">
        <property type="entry name" value="G6P_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001672">
        <property type="entry name" value="G6P_Isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023096">
        <property type="entry name" value="G6P_Isomerase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018189">
        <property type="entry name" value="Phosphoglucose_isomerase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035476">
        <property type="entry name" value="SIS_PGI_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035482">
        <property type="entry name" value="SIS_PGI_2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001211">
        <property type="entry name" value="PRK00179.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11469">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11469:SF1">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00342">
        <property type="entry name" value="PGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00662">
        <property type="entry name" value="G6PISOMERASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00765">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00174">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51463">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="6 10" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ84682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="561" mass="62293" checksum="80052B84895C974F" modified="2010-06-15" version="1">MPFSLASELPAWKTLQDHYHTVKTLHLSDLFAQDPKRAEKFSRSFLNDADGNEIYFDFSKNFLTEETLGLLIKLAKEADVEGLRDRMFKGEKVNFTEERAVYHMALRNVSNKTMEVDGKSVVEDVNSVIEHMKEFSEQVRSGEWKGHTGQRIKSIVNIGIGGSDLGPVMVTETLKPYGHPDLTLHFVSNIDGTHITEALKSCDAESTLFLVASKTFTTAETCTNANTAKEWFLKQKGIDGDRSAIAKHFVALSTNEAEVTKFGINAQNMFGFESWVGGRYSVWSAIGLSVALYIGYDRFHEFLAGAHAIDNHFLSKPLEENIPILGGLLSVWYSNFFNAQTHLVSPFDQYLHRFPAYLQQLSMESNGKSVTRDGSIVNYTTGAILFGEPATNAQHSFYQLLHQGTKFIPTDFILAAESHNPVAPDNKHQLMLASNFFAQAEALMVGKDEGQVLAEGTPEKLKNHKVFRGDRPTTAILAQKITPGALGALIAYYEHLTFTEGAIWNINSFDQWGVELGKSLAKKIGSELEKPGDVSGHDASTNTLINKFKKMSGASKVTYKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJQ2</accession>
    <name>D5GJQ2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">thymidylate synthase</fullName>
            <ecNumber evidence="2">2.1.1.45</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00009125001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>dUMP + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate = 7,8-dihydrofolate + dTMP</text>
            <dbReference type="Rhea" id="RHEA:12104"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57451"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <dbReference type="EC" id="2.1.1.45"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; dTTP biosynthesis.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.45" evidence="2"/>
    <dbReference type="EMBL" id="FN430332">
        <property type="protein sequence ID" value="CAZ84745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840554.1">
        <property type="nucleotide sequence ID" value="XM_002840508.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJQ2"/>
    <dbReference type="FunCoup" id="D5GJQ2">
        <property type="interactions" value="544"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJQ2"/>
    <dbReference type="GeneID" id="9186779"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009125001"/>
    <dbReference type="eggNOG" id="KOG0673">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021669_0_2_1"/>
    <dbReference type="InParanoid" id="D5GJQ2"/>
    <dbReference type="OMA" id="AYGRFWR"/>
    <dbReference type="UniPathway" id="UPA00575"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004799">
        <property type="term" value="F:thymidylate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006231">
        <property type="term" value="P:dTMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00351">
        <property type="entry name" value="TS_Pyrimidine_HMase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.572.10:FF:000002">
        <property type="entry name" value="Possible thymidylate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.572.10">
        <property type="entry name" value="Thymidylate synthase/dCMP hydroxymethylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00008">
        <property type="entry name" value="Thymidy_synth_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045097">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023451">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036926">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000398">
        <property type="entry name" value="Thymidylate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020940">
        <property type="entry name" value="Thymidylate_synthase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002497">
        <property type="entry name" value="PRK01827.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03284">
        <property type="entry name" value="thym_sym"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11548:SF2">
        <property type="entry name" value="THYMIDYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11548">
        <property type="entry name" value="THYMIDYLATE SYNTHASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00303">
        <property type="entry name" value="Thymidylat_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00108">
        <property type="entry name" value="THYMDSNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55831">
        <property type="entry name" value="Thymidylate synthase/dCMP hydroxymethylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00091">
        <property type="entry name" value="THYMIDYLATE_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thymidylate synthase/dCMP hydroxymethylase" evidence="8">
        <location>
            <begin position="17"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ84745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="306" mass="34435" checksum="EAE70F74061F7E2E" modified="2010-06-15" version="1">MSTSSTQRENPTHPEYQYLDLVRNILTNGEHRPDRTGTGTISLFAPPQLRFNLKNHTFPLLTTKRLFHKAILHELLWFISGSTSNAPLSAVGVKIWEGNGSRAYLDSIGLTERAEGDLGPVYGFQWRHFGAKYVDCHTDYSGQGVDQLADVIYKLKNNPYDRRIIMSAWNPADLAKMALPPCHMFAQFYVSYKEGKGMGALSCVLYQRSCDMGLGVPFNIASYALLTIMIAHVCGLEPGEFIHTMGDAHVYADHADALKGQLERAPREFPTLKINRQVDDIDDFKFEDFELVGYDPHPSIKMNMSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJX1</accession>
    <name>D5GJX1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00009251001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430334">
        <property type="protein sequence ID" value="CAZ84814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840623.1">
        <property type="nucleotide sequence ID" value="XM_002840577.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJX1"/>
    <dbReference type="GeneID" id="9186850"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009251001"/>
    <dbReference type="eggNOG" id="ENOG502QTP8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005757_2_0_1"/>
    <dbReference type="InParanoid" id="D5GJX1"/>
    <dbReference type="OMA" id="ETTHHER"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050471">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43433:SF10">
        <property type="entry name" value="AB HYDROLASE-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43433">
        <property type="entry name" value="HYDROLASE, ALPHA/BETA FOLD FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="468"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="180"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="598"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="257"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="371"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="598"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="628"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ84814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="822" mass="88680" checksum="53F6B6DE0B87F3CD" modified="2010-06-15" version="1">MASSIPATPPLPPPQTPHRHSHHPSTNPRQKLQNPRRKSAPSHRGHLSSASTAAASPEVITNLVDSLSKLSPGPLHNPSLYGSLHAPDMFPPQPSPRELDYDYTVGVGGIGREVGFLHPDDAALAPVVRTAKPPSGFSVHTNGSGEGASRRSSYASGISAGKGGVDIPNYVRNYMNRAAGAGDGMADRRNSRESSVSGKSTRRDGGGSRLSYMSSRERMRMEKGRSRDRDVSVERDSERELRMSAAGVLSATSGGDYRGRGKTEEAGGRHIPERRSSYGKGSPQRISSYPAAIVTNVSPMHPGSPLGSPMFPNHEEVDEEDHNGAAPAPDLPRNRSERDRKRSSKQYPKRADSLNSGMGRSPSKAKRRSEPQSILANQFGQQQASPPRPSFQSAPPETTHHERPSSADSIDDAVDSYLCSPRLSQKIRMPTTGRIISFSEVGDPSGFAVFVCVGMGLTRYVTAFYDELALTLGLRLITPDRPGVGESEGIDENERTVLNWPDDVLYICQSLKITKFSILAHSAGAIYALATALRMPGHIRGKIHLLAPWIPPSQIEGVVGTGKEGEEKVGSIPTTQRILRALPTPILKAANSSFMSATSSSLTTSLPRTGGRDRSRKNRRAASAAGGDMRKGDSPGKDRPSKGNTDMPSGPLSPTLAVARNGTGKDGAIVTTAEKPSAEDEVIAAALVSAAERERQMSYDERLTHAIWTLSTRNANPAVDLLVCLERNRPVGFRYVDITRAVVIHHGARDTRVPVENVKWLGKTMRRCEVRILDGEGHGLMASAVVMGGVLDEIAAEWRDWRKVVEGRGEIHVGGLERRWAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJX6</accession>
    <name>D5GJX6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00009259001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="CAZ84819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840628.1">
        <property type="nucleotide sequence ID" value="XM_002840582.1"/>
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    </dbReference>
    <dbReference type="STRING" id="656061.D5GJX6"/>
    <dbReference type="GeneID" id="9186855"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009259001"/>
    <dbReference type="eggNOG" id="KOG2319">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007625_0_1_1"/>
    <dbReference type="InParanoid" id="D5GJX6"/>
    <dbReference type="OMA" id="DVCIAKK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030139">
        <property type="term" value="C:endocytic vesicle"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="CUE_VPS9_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
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    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.80">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR041545">
        <property type="entry name" value="DUF5601"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR041804">
        <property type="entry name" value="Vps9_CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037191">
        <property type="entry name" value="VPS9_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23101:SF25">
        <property type="entry name" value="GTPASE-ACTIVATING PROTEIN AND VPS9 DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23101">
        <property type="entry name" value="RAB GDP/GTP EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02845">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18151">
        <property type="entry name" value="DUF5601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02204">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00546">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00167">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109993">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51140">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51205">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VPS9" evidence="3">
        <location>
            <begin position="337"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="CUE" evidence="2">
        <location>
            <begin position="646"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="508"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="595"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="142"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="521"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="538"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="552"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="595"/>
            <end position="617"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ84819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="691" mass="74904" checksum="3561D3C469FD90AD" modified="2010-06-15" version="1">MGSTPTPIGPEEKEEGESSQPPNTSSEASIDLLASEAGTLSISPLNPESVPAAPMDSLIPGNDGEDYRPPTPLKDDALAKREQDAALSTPPPPPPPKDVPPKDVPPKDVPPKDVPPKDVPPKDRVHLFPSTMPRPSRPCSKSASPSRRGSSQQGRSSGSSSEDEAGDSTRSEIHNIFEQGGAPASTEHLHPSSSASPTLAEAMIGARHPPRTSSLDGADYLARASSVTPSLLEKGKSALSKEPDSHTAHAHTVHQPPPPDEDLPFDFHRFLSQLRHRSADPVAKFLRSFLNEFAKKQWMVHEQVKIVSDFLNFIYGKMETCEVWLNVGEVEMDNAREGMEKLVMNRLYTQAFSPEISPPQANPTHQEDVERDEVLAQKVRIYGWVREEHLDIRDAMGSYRAPRDKVICVLNCCKVIFGLLRHSGGDESADKFVPLLIYVVLRANPANLVSNVQYILRFRNPDKLNGEAGYYLSSLMGAIQFIEGLDRSSLTITNEEFERNVEAAVAQIAERPLPTTTTDASRGDDGEIVNEKGDRSGPSVTPRVSSEQPRFSTSSRESNDSSTSNGDDEKLAVAGLLRSIQRPLTTIGRMFIDEPTTGNATTAAGGPALTPAPGNTPRGSPSAEDKAAARQASAEVEQARMIQRREHESVVQVLQNMFPDLDKEIIADVVRMKQGSVGSAVDACLALSGDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJY6</accession>
    <name>D5GJY6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00009271001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430335">
        <property type="protein sequence ID" value="CAZ84829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840638.1">
        <property type="nucleotide sequence ID" value="XM_002840592.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJY6"/>
    <dbReference type="FunCoup" id="D5GJY6">
        <property type="interactions" value="773"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJY6"/>
    <dbReference type="GeneID" id="9186865"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009271001"/>
    <dbReference type="eggNOG" id="KOG2728">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042326_1_0_1"/>
    <dbReference type="InParanoid" id="D5GJY6"/>
    <dbReference type="OMA" id="LERYQHH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990143">
        <property type="term" value="C:CoA-synthesizing protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004632">
        <property type="term" value="F:phosphopantothenate--cysteine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990181">
        <property type="term" value="P:acetyl-CoA biosynthetic process from pantothenate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10300">
        <property type="entry name" value="CoaB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035929">
        <property type="entry name" value="CoaB-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12290">
        <property type="entry name" value="CORNICHON-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102645">
        <property type="entry name" value="CoaB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="211"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ84829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="350" mass="38787" checksum="AA8A5A8E03A993E8" modified="2010-06-15" version="1">MADSSTSESHYFSSNPPPPTLSQHTAQANAFVTHHHAHSRRVVLLTSGGTTVPLENQTVRFIDNFSAGTRGATSAEYFLSQGYAVIFLHRQFSLLPFSRHYSHSTNCFLDFLSEWGRIGEGEEGEDGGAATPDVVVKAKYRREMLSVLRKYRKAKEQKLLLLLPFTTVTSYLFTLRAIACEMRCLGNEALFYLAAAVSDFFVPPDRMAEHKIQSGDVPESTSGPSPGGGGGGKKLVIDLDPVPKFLKRLVEQWAPEAMIVSFKLETDPALLLKKCNQSLDRYAHHLVIGNILATRKYEVLFVEEGSAQWVRLPTSDGNSGGNEEVEIESLIVPEVIKRHEKMLARKAAIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GK05</accession>
    <name>D5GK05_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00009300001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430335">
        <property type="protein sequence ID" value="CAZ84848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840657.1">
        <property type="nucleotide sequence ID" value="XM_002840611.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GK05"/>
    <dbReference type="STRING" id="656061.D5GK05"/>
    <dbReference type="GeneID" id="9186885"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009300001"/>
    <dbReference type="eggNOG" id="KOG0725">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_442912_0_0_1"/>
    <dbReference type="InParanoid" id="D5GK05"/>
    <dbReference type="OMA" id="HIIAHYW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019301">
        <property type="term" value="P:rhamnose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000417">
        <property type="entry name" value="Glucose 1-dehydrogenase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760:SF83">
        <property type="entry name" value="(3R)-3-HYDROXYACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASES/REDUCTASES FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0684">Rhamnose metabolism</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="113"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="208"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="240"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ84848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="617" mass="68334" checksum="49FF2F994C932F84" modified="2010-06-15" version="1">MSMMDPATELKNLKFTPRTSAWKHMQRFERLCSLAYPRATDAGKWSLFVKTIDTTNVWGAGRGMTPGRWLMDLSEDWLRKNPHCSQPDCTDCKHDSMSFDYLRSAFLERWGNTNTSPHGKLRQPTRSHSLPPPSESDGDPVDVLTPSDFISNALIPRGGSSNYNPPAAPTPPNEPVSERERLVQETKTSIAINPAGNGGGQIVLQITDNNKSRKRKKDIKATIDTPPPQHGMNEGSLPDSPRDRDYHDRDYHHPDDAYRSDRDERDHRTPRDYRSQHHDRERSRHRDGVHVIEAEPPVLELIRQPSPQSDHRHDNSQALVEVASSEVDESTVTGDAMLTAPSQPVLRPRLLEGKICAITGASRGIGRAIALGFAKEGAHIIAHYWGTKSDPANEEIVSLCVEIRSMGQGCTIVFGDISDPRTSDNIVKRAVETYGRLDVAVGNAGMCWYREFLDVTPELLRRHVDVNLNGNFYFVQACARQFKEQFHAMPIEPRPDPIPDYSIIFISSAGALSGNGHQAHYNPTAAGLLSLMQGTTISLGPYGVRCNALLPGVVQTKMNREELQDMEKRGRLEKKVPLGRLGVPSDVVGPAVWLASEGAKYVSGAQIAVDGGAFVNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>D5GK12</accession>
    <name>D5GK12_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00009310001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430335">
        <property type="protein sequence ID" value="CAZ84855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840664.1">
        <property type="nucleotide sequence ID" value="XM_002840618.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GK12"/>
    <dbReference type="GeneID" id="9186892"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009310001"/>
    <dbReference type="HOGENOM" id="CLU_1846567_0_0_1"/>
    <dbReference type="InParanoid" id="D5GK12"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054505">
        <property type="entry name" value="Myb_DNA-bind_8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22980">
        <property type="entry name" value="Myb_DNA-bind_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like DNA-binding" evidence="2">
        <location>
            <begin position="4"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ84855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="139" mass="14356" checksum="2E05E1B84365F9E8" modified="2010-06-15" version="1">MASSDDYKFLLSCLMNHTGKIDFEAVARDTGYTNPRSASNRLASIKKNHAAAMAAAAASGTSSLTASSSKEQPLAIPAVPPPQAQSAAEKSAAKRRMSKKAKRGREGEGGGDGGDDGDPEVVKGSKKRKGTTTGDDEDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>D5GK80</accession>
    <name>D5GK80_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00009418001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430337">
        <property type="protein sequence ID" value="CAZ84923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840732.1">
        <property type="nucleotide sequence ID" value="XM_002840686.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GK80"/>
    <dbReference type="STRING" id="656061.D5GK80"/>
    <dbReference type="GeneID" id="9186952"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009418001"/>
    <dbReference type="eggNOG" id="ENOG502QTJE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050424_0_1_1"/>
    <dbReference type="InParanoid" id="D5GK80"/>
    <dbReference type="OMA" id="VWAFWSS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022057">
        <property type="entry name" value="Chs7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35329">
        <property type="entry name" value="CHITIN SYNTHASE EXPORT CHAPERONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35329:SF1">
        <property type="entry name" value="CHITIN SYNTHASE EXPORT CHAPERONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12271">
        <property type="entry name" value="Chs7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="180"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="219"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="251"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="294" mass="32275" checksum="CA483EFD21C119C0" modified="2010-06-15" version="1">MSQFGNFHDFCRDSTLPVCNLFYNGEKQCLLMGFKAGDGRVANLGSIILCVVAIIVSVLLLLLSQKKRAAVGRREMQIFLILYILVSLAEVFTVGGFLTNRTVLVWFTGIHLGAIAATAWVLLLNAIVGFQLVDDGTPLSVGLVAGSALAFFIGTAYVAVDTGFGEPGNFPVKDPDELKNYALYVLYLLFPLIAIVAFFFLEAFLVLRVLGEIMPMVRLGVSALFFVIGQIFNFIISVHLCDVSNGKIDGALFETLFTLLAVVTLWFFWSSITEDEWPDDDPMNPIISDSVYSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKB9</accession>
    <name>D5GKB9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Elongator complex protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00009468001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ELP5 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430337">
        <property type="protein sequence ID" value="CAZ84962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840771.1">
        <property type="nucleotide sequence ID" value="XM_002840725.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKB9"/>
    <dbReference type="STRING" id="656061.D5GKB9"/>
    <dbReference type="GeneID" id="9181982"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009468001"/>
    <dbReference type="HOGENOM" id="CLU_050414_2_0_1"/>
    <dbReference type="InParanoid" id="D5GKB9"/>
    <dbReference type="OMA" id="VEMEYRR"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019519">
        <property type="entry name" value="Elp5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15641">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15641:SF1">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10483">
        <property type="entry name" value="Elong_Iki1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="86"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="105"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ84962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="116" mass="12936" checksum="34825E4F0A16FCF0" modified="2010-06-15" version="1">MVVEMEYRRKSGRAVVEWFVIGGGQGKGGVILLEDHPLWRVSEGVRKGEAGELEENNGIDGITFELGITERQRRVREEVQLPYMDAQREGGIGGGGAILFTPEKEADDFDEEEDEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKC8</accession>
    <name>D5GKC8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00009481001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430337">
        <property type="protein sequence ID" value="CAZ84971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840780.1">
        <property type="nucleotide sequence ID" value="XM_002840734.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKC8"/>
    <dbReference type="FunCoup" id="D5GKC8">
        <property type="interactions" value="939"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GKC8"/>
    <dbReference type="GeneID" id="9181991"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009481001"/>
    <dbReference type="eggNOG" id="KOG0087">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_23_0_1"/>
    <dbReference type="InParanoid" id="D5GKC8"/>
    <dbReference type="OMA" id="ITAIYQM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01868">
        <property type="entry name" value="Rab11_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000085">
        <property type="entry name" value="Putative ras-related protein rab-11a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="182"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ84971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="212" mass="23639" checksum="2ACACCA953FB60FA" modified="2010-06-15" version="1">MANDEYDFLFKVVLIGDSGVGKSNLLSRFTRNEFNLDSKSTIGVEFATRSIQVDTKTIKAQIWDTAGQERYRAITSAYYRGAVGALLVYDISKQITYDNVTRWLKELRDHADNNIVIMLVGNKSDLRHLRAVPTEDAKNFAAENHLSFIETSALDSTNVELAFQTILTEIYRIVSSKALDGGDQHQVSQPGSSQPLHITPTAENSTKDTKCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKF7</accession>
    <name>D5GKF7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00009516001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nudE family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430338">
        <property type="protein sequence ID" value="CAZ85000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840809.1">
        <property type="nucleotide sequence ID" value="XM_002840763.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKF7"/>
    <dbReference type="STRING" id="656061.D5GKF7"/>
    <dbReference type="GeneID" id="9182020"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009516001"/>
    <dbReference type="eggNOG" id="KOG1853">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034391_0_0_1"/>
    <dbReference type="InParanoid" id="D5GKF7"/>
    <dbReference type="OMA" id="NMAIERS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051642">
        <property type="term" value="P:centrosome localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000132">
        <property type="term" value="P:establishment of mitotic spindle orientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047496">
        <property type="term" value="P:vesicle transport along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033494">
        <property type="entry name" value="NUDE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006964">
        <property type="entry name" value="NUDE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10921:SF1">
        <property type="entry name" value="NUCLEAR DISTRIBUTION PROTEIN NUDE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10921">
        <property type="entry name" value="NUCLEAR DISTRIBUTION PROTEIN NUDE HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04880">
        <property type="entry name" value="NUDE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NUDE" evidence="9">
        <location>
            <begin position="129"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="203"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="476"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="10"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="130"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="217"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="254"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="274"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="394"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="416"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ85000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="523" mass="57699" checksum="5C15C71C707F8D48" modified="2010-06-15" version="1">MPSKDCDSPGSEAKEALAYYKSQIQQLESELVDFQASSKELEQELEKELEASEKQHRDLRNKNEGLRYEVEEWKNKYKQSKTEANSAQNTLQKEITNLRETNRQISLRLRDIEVSNDDFERQERIVKSSLEDMESKYNQAIERGVLLEEEIKIGEQEREGLRIECQRLRDELSDLKVEQDITVDKLNNAMAMAAKYGNGSLHHVPPPAKFQSTLSETSSVASRPPSTPTTPSAASTSTPQSDHSPTPPSPPMSEVSTTPSQPSRAREDPALTPRPKHHQRPAHSRAPSFGSHGPGMRPPIPSSKSLHHIRGLIGQMQRLEQRVQHARSKLPGPVNTPPRISPRNQGAFIPPTVTMRSQKKHRPSSISSVTSGRSDRDTVYPHGTRLSFGERPSSRATSRPSSRASGATSRSSLGSQLPQSHRPSSRASLSGRVTPMETHSEFGMTTINVRDRTSIGSVASSTDDTFPAPAIRRNTVSKGESGIPLPASGLPRRSFGGRRISSTSVEGEARRRLGKKLSGVGEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKJ3</accession>
    <name>D5GKJ3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00009591001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430339">
        <property type="protein sequence ID" value="CAZ85036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840845.1">
        <property type="nucleotide sequence ID" value="XM_002840799.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKJ3"/>
    <dbReference type="GeneID" id="9184697"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009591001"/>
    <dbReference type="HOGENOM" id="CLU_1289778_0_0_1"/>
    <dbReference type="InParanoid" id="D5GKJ3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="214" mass="23734" checksum="F560D462118EA384" modified="2010-06-15" version="1">MTGTGTFELALIDTSCLEPPRKKRRLTHAVALDLFDSPRPSSPEPPHEKTPYPHGGRKGGDDNDEMMTDGILPVAAVPDVISTAYDDYVSTLPLNILFQVKVTNNATAREAFPDALLGHSIRLYYPEYKSFARFMREGVIAGLRLESARDVRNIKIVARKAGEVIVELDILSDGEVWEKAMRELRKIGIGKHGEGGRKMASSVRLTLGLNYWGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKK9</accession>
    <name>D5GKK9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00009616001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430340">
        <property type="protein sequence ID" value="CAZ85052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840861.1">
        <property type="nucleotide sequence ID" value="XM_002840815.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKK9"/>
    <dbReference type="GeneID" id="9184714"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009616001"/>
    <dbReference type="eggNOG" id="ENOG502RYRX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019101_0_2_1"/>
    <dbReference type="InParanoid" id="D5GKK9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF19">
        <property type="entry name" value="MEMBRANE PROTEIN PTH11-LIKE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G14080)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="104"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="7">
        <location>
            <begin position="95"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ85052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="253" mass="27922" checksum="7AB5926943002AF1" modified="2010-06-15" version="1">MIGALALLVGRVVVVHLVIVNGTNDLRGIGELGGEEGRRREFGSKMILIGRTCYALFLWFLKFGIVSLYERIAPQSKGYRVPLKCLSTGQVYPDPGETCHYSEIQLYTSGACNIVTDLILMIYPLPIIFRSRIPTRRKLQIGGLFSLTFFAILASTLRLPFVMSNNSRQRWRALFAWVEMLVACFVANAPDLYRAIGVRANGSSGTRSSGEGAPGRYWFTANRRRGIRGRGVAPALREEGESEGVSGGSLELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKM1</accession>
    <name>D5GKM1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="14">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00009638001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MscS (TC 1.A.23) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430340">
        <property type="protein sequence ID" value="CAZ85064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840873.1">
        <property type="nucleotide sequence ID" value="XM_002840827.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKM1"/>
    <dbReference type="STRING" id="656061.D5GKM1"/>
    <dbReference type="GeneID" id="9184726"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009638001"/>
    <dbReference type="eggNOG" id="KOG4629">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010480_1_0_1"/>
    <dbReference type="InParanoid" id="D5GKM1"/>
    <dbReference type="OMA" id="FWWLGIE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097038">
        <property type="term" value="C:perinuclear endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015275">
        <property type="term" value="F:stretch-activated, monoatomic cation-selective, calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006884">
        <property type="term" value="P:cell volume homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000345">
        <property type="entry name" value="Mechanosensitive ion channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_beta-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058650">
        <property type="entry name" value="Msy1/2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31323:SF15">
        <property type="entry name" value="MECHANOSENSITIVE ION CHANNEL PROTEIN MSY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31323">
        <property type="entry name" value="MECHANOSENSITIVE ION CHANNEL PROTEIN MSY2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25886">
        <property type="entry name" value="Msy1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="3" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="9" id="KW-0407">Ion channel</keyword>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="160"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="239"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="278"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="497"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="534"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="13">
        <location>
            <begin position="441"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="87"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="738"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="819"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="87"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="825"/>
            <end position="841"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ85064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="907" mass="100615" checksum="87BC54AF636F2F43" modified="2010-06-15" version="1">MSTPVHGIPQPTCPNIQVTANTPGRPPPPHVGGCSSENEVFASENPPEPQKRASNISDGEEICPPPTRREENYRLEDDLELLRAERVVSRASEHTGDLSRSKSGRHRSRPRTPEPVDDFDINTNPAHDGGKGVWRPPERPATRFGKVLKAVHGSSVLVRYFTYIVPLVLILLIPLLLGALVFREATVGDVYLMWFMVWLEIVWLSLWAGRILAKCVPYVMDIISGAFTNNSRKWKDMGCMLEVPATLFFWWLAVYVSFLPTMTNHHNDGDNRTRYWESRASIVLLALFIAMILNLFEKIIIQLIAISFHQRTYEDRIDLNKFQISSLAKLYAHSKEVAGGRDLDEKQGSGLTSGAKTPLVVFQHAKAGAHSAFTKVGDVMGKVAGDFTGRQVSSSTSPQQVVLTLLYTTEGSQALARRLFRTLVREGTEVVSAEDLRHVFTSEEEAEAAFQMFDRDLNGDISCEEMEIACVEIGRERKAITASLKDLDSVVSKLDDVFTFLVTVAVILIFLSLISKSTAGVLTSASSSVLALSWLFSATAQEFLASIIFVFVKHPFDVGDRVDVYNTGAGTVDTFFVKEIALMYTEFKKLEGHVVQAPNSLLNTLFILNMRRSGALAEAIPIVCKFGTSLEQIEELQERLLAFVKFENREYQGKVITELSRDVPDMHSVKLNVVFFYKSNWQNELVRLQRRNKFMCALMVSAADLGIESPNMRWPGQKQTSPVFLQSVNADQIQQSVLDRDGGPSGKGGEYRTFAPPAEPHGILRSESRAGLGRSASRGSTKKVDFSLGVKDIAPSDDSGDVFEDNRRNIPAIQLMRVLEEEEGQERRDALSRTTSRDSSIDRNPGMGGAVNRRRSGSGSIASYGNRFFGGRSRNSIDLEGGEGPSSPVRWTSRGGSARDSIEMGRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKQ4</accession>
    <name>D5GKQ4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">separase</fullName>
            <ecNumber evidence="2">3.4.22.49</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00009703001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>All bonds known to be hydrolyzed by this endopeptidase have arginine in P1 and an acidic residue in P4. P6 is often occupied by an acidic residue or by a hydroxy-amino-acid residue, the phosphorylation of which enhances cleavage.</text>
            <dbReference type="EC" id="3.4.22.49"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.4.22.49" evidence="2"/>
    <dbReference type="EMBL" id="FN430340">
        <property type="protein sequence ID" value="CAZ85097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840906.1">
        <property type="nucleotide sequence ID" value="XM_002840860.1"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GKQ4"/>
    <dbReference type="GeneID" id="9182342"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009703001"/>
    <dbReference type="eggNOG" id="KOG1849">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000454_0_0_1"/>
    <dbReference type="InParanoid" id="D5GKQ4"/>
    <dbReference type="OMA" id="FWSRYIK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051307">
        <property type="term" value="P:meiotic chromosome separation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005314">
        <property type="entry name" value="Peptidase_C50"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030397">
        <property type="entry name" value="SEPARIN_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12792">
        <property type="entry name" value="EXTRA SPINDLE POLES 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12792:SF0">
        <property type="entry name" value="SEPARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03568">
        <property type="entry name" value="Separin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51700">
        <property type="entry name" value="SEPARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase C50" evidence="6">
        <location>
            <begin position="1909"/>
            <end position="2004"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="109"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="220"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1336"/>
            <end position="1370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="129"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="242"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1348"/>
            <end position="1360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1361"/>
            <end position="1370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ85097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="2097" mass="231504" checksum="F3A44137EA46FF1B" modified="2010-06-15" version="1">MSSKIAASSNPYDEIKQSLGAETCPASTVKLLNEALFAPASSMVAATLKVSTRTNHSRTNSRSQKTVVAVGSASVAVTLASRTADRYKLAIEVINTSLRNLSEAVKKDPKATVLEVHEKDQPVRKTEKQQQTSVARSSHSRPSNRNDAPTLLRPDATENLAICCSLAISYLNSISGSSNALDIAPLQIENAQNSLILKLIQLGMFELGLKESRSLKRTLAEAMRSGGGSEKQGKPTPAVALAEKEDSRRNVSKAQKEPIDKLLEFDDVVTSSPAFPLVISCQLGILRCIAGLKRPELVEAVLPTLSSSTVHNPLNVIRALAGTSLQKALAHLNVLHYTLVSMAPSISTSIDAVATNAKLSCSPRAALALQTKALQCSIVKLTLQPNFTEITDKVREEHWESWRRYASAFVRRTQDYSNKRERYGIVRDLADAVRRFLRVEGASHSYSPEVTRILSSLSFEAGLMTQSMELTRAWVQELEKSDVQSDAALELVGRVRMATAELKISMVMEDGELLVDGSGVQRIKDRVGTAVEGLEKISKGRRSDLDQLLVEAGYLRRSGVSLLVALLKDESPGFIELKESEARTVRLELRDVCNMIARGVVGFCRKYLAMGSQGDERTKAIKLAVSAIDTSISTAWRGFDIEYCAQWEGLEELTRDSLALVRSLTESDLEALGDLTLYEKFSSTYWQIHLLYRKAGMDKESVRALKRSISVLDGRPPAQLVKANIALKWERLGSSFMSTGDWRRAEETLLKAMEVFTETGIIGELGICASKGDRIGSEFEVHTQEAMVGRVLASLIKCASRRKSTEVAALRFDDEKIAAAARGMLLEWSLKLAIGVMANDGVVVRVLAERLLEAYPLEAMPLRRARVVSRILCLAVDHPEIFNIEEVRVIGDEVASWSRETPIPSLAEDGNLAKYKEDIIAGCLVSLAFLKWINGEAGVESVKEAVGAWGTLVKGCSSWDMLLEKVEEISVIMKTLDMLAEFSEMRGEADLKMCVLKVLVSLREIEKQADYDALVRLETLIGLHYLRLGYSGRSGMTMAKAQALMKKDQASVSTVTILQWHMAYTEYLVNIGNLEKAEEYLNAASNILEEDQDLHYAGMSGARIAKRVKLNRIIADAAYVMSLLTFEKGNPSDALAHARRCVRLNQRAWAGLESLSRDSLVDSMATLSTSDYPQPKVQSTTFGALNVPLLWPLVSSLYAGLLQTSNIYRHQGMVREAEFYLEQALKTVEAVNASPRVSLAMTMYGDLRIRSGHIDEGEEMLQKAGKSIGGGREALEFEVASGNLDRLRGDFAGEGRAYERAEKQLEQLMSVRYIEKLGALTGDSEGLSEKVAILELREGPQPGPEPKRRGRPPAKPKTTKASKASKVTKASRGVPAISSVVAAECSVLLKQKGNILRLKAHNLTMQRQCEQAEVLLLEASKLPVGHQETVIHGLTEAKHLLQEALSLVGSDPVFSVLHESTISLPSVAPRTGESSPVKKSTKKGVAAAVKQTKKFVEILTRARDAVANVHTRAVKMGSSTITHSVASLLSQIIVLLSAVTNSKGKGPEHPLFASYSLELHKGISLQREKAAIETEKVVSRIPEDLSWPRAGSVPDAPKSLLAGSAPFEFSNFQSDYVDIIPKKWAAVSVSLSESREELYLSRFQAGQSQLMVRLPLTRHNSRDDYEDRWEYESMLEELNEIVKGANISAHNAKDMTSKGARTEWWKQRESLDTQLKDLLSVIEYHWLGGFKGVFCQYALHKEYMAKFKSAFDKVLARHLPSRQNRRAKRVNIDYRILELFIGLGRPDEQDLDEALVDLIYFVVDILQFHGEGNAYDEVDFDVMVLELTEALAAYHKGVSFHPNEVASDVDHTILILDKSVHMFPWESLPCLRGHSVSRLPSLASLRDRILMMQEKEPSQQSRPGFYADKTRGAYILNPGSDLVNTQKAFEDDLRTLKTWEGIVGRPPSEEEFRRNLEERDLLLYFGHGSGAHFIRARTLKKLDKCGIALLLGCSSGALKDTGEFEPYGMPVNYLLGGCPSMVATLWDVTDKDIDRFSKDVFNRWGLFSGPGKSKKERELEAPNASLVEAVAKGRESCVLKYLNGAAPVVYGIPAYLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5GL20</accession>
    <name>D5GL20_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Peroxidase</fullName>
            <ecNumber evidence="14">1.11.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">GSTUM_00009918001</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Destroys radicals which are normally produced within the cells and which are toxic to biological systems.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>2 Fe(II)-[cytochrome c] + H2O2 + 2 H(+) = 2 Fe(III)-[cytochrome c] + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:16581"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.11.1.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Forms a one-to-one complex with cytochrome c.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion intermembrane space</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peroxidase family. Cytochrome c peroxidase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.-" evidence="14"/>
    <dbReference type="EMBL" id="FN430344">
        <property type="protein sequence ID" value="CAZ85213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841022.1">
        <property type="nucleotide sequence ID" value="XM_002840976.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GL20"/>
    <dbReference type="FunCoup" id="D5GL20">
        <property type="interactions" value="76"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GL20"/>
    <dbReference type="PeroxiBase" id="11112">
        <property type="entry name" value="TmelCcP01"/>
    </dbReference>
    <dbReference type="GeneID" id="9185807"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009918001"/>
    <dbReference type="eggNOG" id="ENOG502QR1E">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036959_1_1_1"/>
    <dbReference type="InParanoid" id="D5GL20"/>
    <dbReference type="OMA" id="QRKWNGP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004130">
        <property type="term" value="F:cytochrome-c peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000302">
        <property type="term" value="P:response to reactive oxygen species"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00691">
        <property type="entry name" value="ascorbate_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.420.10:FF:000009">
        <property type="entry name" value="Ascorbate peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.520.10:FF:000005">
        <property type="entry name" value="Cytochrome c peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044831">
        <property type="entry name" value="Ccp1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002207">
        <property type="entry name" value="Peroxidase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019794">
        <property type="entry name" value="Peroxidases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019793">
        <property type="entry name" value="Peroxidases_heam-ligand_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31356:SF58">
        <property type="entry name" value="CYTOCHROME C PEROXIDASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31356">
        <property type="entry name" value="THYLAKOID LUMENAL 29 KDA PROTEIN, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00459">
        <property type="entry name" value="ASPEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00435">
        <property type="entry name" value="PEROXIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00436">
        <property type="entry name" value="PEROXIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0349">Heme</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5 14" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="16">
        <location>
            <begin position="172"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="211"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAZ85213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="377" mass="41363" checksum="19D37CF93D013DAD" modified="2010-06-15" version="1">MASMARVLTRASKQASFTSTPLCRNFTTATRARPTTSIAAPIRQTGRRGYASGGSAGGGSSKIAIPLGLGLIGAGGYYAYTNDLLPAALSGAQASKTGGVLTPTKDDFQKVYNDVAELLENNDYDDGSYGPVVVRLGWHASGTYDKDTKTGGSNGATMRFAPESEHGANAGLKTARDLLEGIKKKHPWISYSDLWTLAAVAAIQEMGGPKIPWRPGRKDGDVSACTPDGRLPDATKEQKHLRAIFYRMGFNDQEIVALSGAHALGRCHTDRSGFDGPWTFSPTMLTNDYYKLLLEEKWAWKKWNGPKQFEDVSTKSLMMLPTDMALVKDKEFRKHVERYAKDNDLFFREFADAFGRLLELGVPFEKEEKFVFKSTTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>D5GL71</accession>
    <name>D5GL71_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Lon protease homolog</fullName>
            <ecNumber evidence="9">3.4.21.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">GSTUM_00010035001</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="9 12">Belongs to the peptidase S16 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.-" evidence="9"/>
    <dbReference type="EMBL" id="FN430346">
        <property type="protein sequence ID" value="CAZ85264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841073.1">
        <property type="nucleotide sequence ID" value="XM_002841027.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GL71"/>
    <dbReference type="STRING" id="656061.D5GL71"/>
    <dbReference type="GeneID" id="9182058"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010035001"/>
    <dbReference type="eggNOG" id="KOG2004">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004109_4_0_1"/>
    <dbReference type="InParanoid" id="D5GL71"/>
    <dbReference type="OMA" id="MPMNQEY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19500">
        <property type="entry name" value="RecA-like_Lon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.5270:FF:000002">
        <property type="entry name" value="Lon protease homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000091">
        <property type="entry name" value="Lon protease homolog 2, peroxisomal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000039">
        <property type="entry name" value="Lon protease homolog 2, peroxisomal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000382">
        <property type="entry name" value="Lon protease homolog 2, peroxisomal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.5270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.40">
        <property type="entry name" value="LON domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004815">
        <property type="entry name" value="Lon_bac/euk-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054594">
        <property type="entry name" value="Lon_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008269">
        <property type="entry name" value="Lon_proteolytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027065">
        <property type="entry name" value="Lon_Prtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003111">
        <property type="entry name" value="Lon_prtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046336">
        <property type="entry name" value="Lon_prtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00763">
        <property type="entry name" value="lon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10046">
        <property type="entry name" value="ATP DEPENDENT LON PROTEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05362">
        <property type="entry name" value="Lon_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22667">
        <property type="entry name" value="Lon_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02190">
        <property type="entry name" value="LON_substr_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001174">
        <property type="entry name" value="Lon_proteas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00830">
        <property type="entry name" value="ENDOLAPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00464">
        <property type="entry name" value="LON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51787">
        <property type="entry name" value="LON_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51786">
        <property type="entry name" value="LON_PROTEOLYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="2 9 12" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9 12" id="KW-0720">Serine protease</keyword>
    <keyword evidence="7" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="Lon N-terminal" evidence="15">
        <location>
            <begin position="9"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="Lon proteolytic" evidence="14">
        <location>
            <begin position="693"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="302"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="319"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="active site" evidence="10 12">
        <location>
            <position position="786"/>
        </location>
    </feature>
    <feature type="active site" evidence="10 12">
        <location>
            <position position="829"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="450"/>
            <end position="457"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR001174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001174-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001174-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAZ85264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="888" mass="97271" checksum="C94DB44999578DB7" modified="2010-06-15" version="1">MGTYKPNTLPLIPLPYSAVLFPGVILRIFISDRQDIAALVAKLSNNNTTPLNSSDLRIGCIPLKPPTPVSPPIAKTDEDKKRIIEEEGDEVEETQPAFLNIDPSSATPEELFSHGTTAQVVGLEGHSRVDSFGTHGANGGNGMALVVEGVSRFRVRQFLQRTPFIEADVEHFVDSPVQKSDVPAHSYFTQLKTLSRELVALLQMNSSRGVGLPPLVARRLELLIAEKKLCDAGSLADFMVSAVETTLAERLRILSAVDVPERLEKAVTVLTRQVNKIKSILTRSNKILPPLIVMDRRDGLPSLPGVPPRSAKRSAGFGDMDEDGENDEELDELSKKIEEAKLSPEALKLARREMQRLRRMSPIQAEYGVCRTYLETLTEIPWSITTEDQLDATTLVRARKQLNDDHYGLEAIKKRLLEYLAVLRLKRQNDEPVIAKKPLVDKSPILLLVGPPGVGKTSLARSVASALGRKFHRISLGGVRDEAEIRGHRRTYVAAMPGLIVNALKKVGVANPVILLDEIDKIGGANFHGDPSAAMLEVLDPEQNWSFTDHYINIPIDLSKVLFIATANSVDTIPPPLLDRMERIQLSGYTSLEKMHIASQYLIPKQVQSNGLAAGQVEISADVLSTIITSYTRESGVRNLEREIGSVCRAKAVEYAEAKDAHKEESYRPRVEVSELESILGIEKYYSEIAERSNKPGVVTGLVAFSTGGIGSILFIEAEQMPGSGRLQLTGKLGEVIKESVEVALTWVKAHAYVLGLTNHPDEDLTKNRNVHVHCPAGAVPKDGPSAGVAFTIAMISMFSGRRISPTLAMTGEVSLRGRVTPVGGIKEKLIGALTAGVKTVLLPAHNRKEVRELPEEVKNGLEIIYVRSVWEALKHGMRPLTLLVPFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLG4</accession>
    <name>D5GLG4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00010182001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430348">
        <property type="protein sequence ID" value="CAZ85357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841166.1">
        <property type="nucleotide sequence ID" value="XM_002841120.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLG4"/>
    <dbReference type="STRING" id="656061.D5GLG4"/>
    <dbReference type="GeneID" id="9186101"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010182001"/>
    <dbReference type="eggNOG" id="ENOG502S4R7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_132811_0_0_1"/>
    <dbReference type="InParanoid" id="D5GLG4"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029164">
        <property type="entry name" value="PIG-Y"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39400:SF1">
        <property type="entry name" value="PIG-P DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39400">
        <property type="entry name" value="YALI0E29227P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15159">
        <property type="entry name" value="PIG-Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="122" mass="13423" checksum="92B00776CA3327F4" modified="2010-06-15" version="1">MSGGGGRRSRTSSFILLPRQQEIESAADTAYYGFVVLGATWIVFVVGMGSALGIWEWAWDVPDSPFLKIANESDDDKEVLIPGYYPALVILTCVMAWVWVVVAWMGMKYFKHAKIQGGGDDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLT3</accession>
    <name>D5GLT3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00010407001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS10 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430351">
        <property type="protein sequence ID" value="CAZ85500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841309.1">
        <property type="nucleotide sequence ID" value="XM_002841263.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLT3"/>
    <dbReference type="FunCoup" id="D5GLT3">
        <property type="interactions" value="768"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GLT3"/>
    <dbReference type="GeneID" id="9186484"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010407001"/>
    <dbReference type="eggNOG" id="KOG0900">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122625_0_0_1"/>
    <dbReference type="InParanoid" id="D5GLT3"/>
    <dbReference type="OMA" id="IHKRVIH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.600:FF:000002">
        <property type="entry name" value="40S ribosomal protein S20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.600">
        <property type="entry name" value="Ribosomal protein S10 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00508">
        <property type="entry name" value="Ribosomal_uS10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001848">
        <property type="entry name" value="Ribosomal_uS10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018268">
        <property type="entry name" value="Ribosomal_uS10_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027486">
        <property type="entry name" value="Ribosomal_uS10_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036838">
        <property type="entry name" value="Ribosomal_uS10_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005729">
        <property type="entry name" value="Ribosomal_uS10_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01046">
        <property type="entry name" value="uS10_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11700">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S10 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00338">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00971">
        <property type="entry name" value="RIBOSOMALS10"/>
    </dbReference>
    <dbReference type="SMART" id="SM01403">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54999">
        <property type="entry name" value="Ribosomal protein S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00361">
        <property type="entry name" value="RIBOSOMAL_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS10" evidence="4">
        <location>
            <begin position="21"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ85500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="117" mass="13182" checksum="0E9C9EE0C60A54E7" modified="2010-06-15" version="1">MSYNKPEKGEFGDGPKTHRIRITLTSRKVQSLEKVTTDLIERAKSKNLRVKGPVRLPTKVLKVTTRKTPCGEGSKTWDCFEMRIHKRLIDLNAPTEVVKQIIINIEAGVEVEVTIAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLW0</accession>
    <name>D5GLW0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00010441001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430351">
        <property type="protein sequence ID" value="CAZ85527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841336.1">
        <property type="nucleotide sequence ID" value="XM_002841290.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLW0"/>
    <dbReference type="FunCoup" id="D5GLW0">
        <property type="interactions" value="1141"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GLW0"/>
    <dbReference type="GeneID" id="9186511"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010441001"/>
    <dbReference type="eggNOG" id="KOG0127">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011608_3_0_1"/>
    <dbReference type="InParanoid" id="D5GLW0"/>
    <dbReference type="OMA" id="FTHRHAL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12676">
        <property type="entry name" value="RRM3_Nop4p"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Related to Nucleolar protein NOP4"/>
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        <property type="entry name" value="Nop4p_RRM3"/>
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        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
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    <dbReference type="PANTHER" id="PTHR48039">
        <property type="entry name" value="RNA-BINDING MOTIF PROTEIN 14B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48039:SF5">
        <property type="entry name" value="RNA-BINDING PROTEIN 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="match status" value="4"/>
    </dbReference>
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        <property type="match status" value="3"/>
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        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="47"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="153"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="321"/>
            <end position="422"/>
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    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="495"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="236"/>
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        <location>
            <begin position="635"/>
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        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="7"/>
            <end position="26"/>
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    </feature>
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        <location>
            <begin position="245"/>
            <end position="266"/>
        </location>
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            <begin position="288"/>
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            <begin position="666"/>
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        <location>
            <begin position="688"/>
            <end position="713"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="717"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ85527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="732" mass="81577" checksum="82382EE2F177FCB1" modified="2010-06-15" version="1">MAKHAKTNNEDNRPSDDSEVEGLERKERRKKKSKSQDGAEDDTLKRRTVFIRSLPYTATTESLSTHFSFIAPLKHATVVADPVTKKSRGFGFVTFLDPEDAQKAVKQFNGAEFGGRRLKVEIAEKRHRETALSEGEVKKGKTVDGEGVKKRAPRLIVRNLPWSVKKPEDLVKIFQSYGKVRGVIIPRKGNMPNGPMSGFAFVTMKGYKNAENAIEKTNGMEIDGRTVAVDWAAEKNEWEQKKEAEDMDIDGDEEEKGEDAAEDSDEGSGVGVIGDDDAESMDNASDASSDEGSDIEDFDDDEDERNGTQKKFYSVEEEKSLTVFIRNLPFSTDDETLHEHFKSSFGPVRYARIVMDHATERPRGTGFVCFFNKEDCDRCLADAPHQQFLATKGKSLLQNEGDDPSGRYTIDGRILQLTRAVNKAEATKLQEAGLAQRDKAQGDKRRLFLLQEGTILASSPAFQQLSNSERLLREASLKQRKTLLQSNPMLHLSLTRLSIRNLPRSITAKDLKQLAREAAVGFAADAKAGKRKRLSKEELIRGGDEDREAERRRKAQGKGIVRQAKFVEEKAGAGRSRGYGFIEYSSHRWALMGIRWLNGHEGKGKGTVPPPAIEEKKKLIVEFAIENAQVVARRKEKEEKARERSKLVAEGKAKPLESTKEKKQFAAKGKAAVKARGGGKGFSGGKRKREEMATDVRESPSKNVKKDEGEAKAARIIARKRQVRRRAGRGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>D5GM67</accession>
    <name>D5GM67_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein phosphatase PP2A regulatory subunit B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00010546001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the phosphatase 2A regulatory subunit B family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430352">
        <property type="protein sequence ID" value="CAZ85610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841419.1">
        <property type="nucleotide sequence ID" value="XM_002841373.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GM67"/>
    <dbReference type="FunCoup" id="D5GM67">
        <property type="interactions" value="718"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GM67"/>
    <dbReference type="GeneID" id="9187394"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010546001"/>
    <dbReference type="eggNOG" id="KOG1354">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021713_3_3_1"/>
    <dbReference type="InParanoid" id="D5GM67"/>
    <dbReference type="OMA" id="NQIKWCR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005934">
        <property type="term" value="C:cellular bud tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000705">
        <property type="term" value="P:achiasmate meiosis I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071475">
        <property type="term" value="P:cellular hyperosmotic salinity response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045143">
        <property type="term" value="P:homologous chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016237">
        <property type="term" value="P:microautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044818">
        <property type="term" value="P:mitotic G2/M transition checkpoint"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001100">
        <property type="term" value="P:negative regulation of exit from mitosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010515">
        <property type="term" value="P:negative regulation of induction of conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031030">
        <property type="term" value="P:negative regulation of septation initiation signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000786">
        <property type="term" value="P:positive regulation of autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010971">
        <property type="term" value="P:positive regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905477">
        <property type="term" value="P:positive regulation of protein localization to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900182">
        <property type="term" value="P:positive regulation of protein localization to nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061586">
        <property type="term" value="P:positive regulation of transcription by transcription factor localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090266">
        <property type="term" value="P:regulation of mitotic cell cycle spindle assembly checkpoint"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000160">
        <property type="entry name" value="Protein phosphatase PP2A regulatory subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000009">
        <property type="entry name" value="PP2A_PR55"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018067">
        <property type="entry name" value="PP2A_PR55_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11871">
        <property type="entry name" value="PROTEIN PHOSPHATASE PP2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037309">
        <property type="entry name" value="PP2A_PR55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00600">
        <property type="entry name" value="PP2APR55"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01024">
        <property type="entry name" value="PR55_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01025">
        <property type="entry name" value="PR55_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU331113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ85610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="427" mass="48861" checksum="EF8121AEA4AFB179" modified="2010-06-15" version="1">MVDGEIASPTWKFTQCFGDKGDVEDITEADIISTVEFDHTGNYLATGDKGGRVVLFERNETKKTCEYKFHTEFQSHEPEFDYLKSLEIEEKINKIKWCKRQNASHFLLSTNDKTIKLWKVFEKSLKPPPTFNNSSALRLPRLTHHDTVVAAVPRRTYANAHAYHINSISVNSDGETFISSDDLRINLWNLNIQDQSFNIVDIKPANMEELTEVVTAAEFHPQYCNWFMYASSKGTIKLADMRESALCDQHVKLFEQEEDPTSRSFFSEIISSISDVRFSSDGRYILSRDYLTVKIWDINMENRPVKTIPIHEHLRPRLCDTYENDSIFDKFEVVFSGDGNNVMTGSYNNNFMIYPSDPEKEVEVVLQADKSAFKAKKVGVPTPINGGKKGDADQIDFNKKILHMSWHPFEDSIAIAATNNLFVFSAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMB0</accession>
    <name>D5GMB0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL3m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00010632001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL3 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430354">
        <property type="protein sequence ID" value="CAZ85653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841462.1">
        <property type="nucleotide sequence ID" value="XM_002841416.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMB0"/>
    <dbReference type="FunCoup" id="D5GMB0">
        <property type="interactions" value="792"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GMB0"/>
    <dbReference type="GeneID" id="9187263"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010632001"/>
    <dbReference type="eggNOG" id="KOG3141">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044142_0_1_1"/>
    <dbReference type="InParanoid" id="D5GMB0"/>
    <dbReference type="OMA" id="GKNIPCT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000004">
        <property type="entry name" value="50S ribosomal protein L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.810:FF:000001">
        <property type="entry name" value="50S ribosomal protein L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.810">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01325_B">
        <property type="entry name" value="Ribosomal_uL3_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000597">
        <property type="entry name" value="Ribosomal_uL3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019927">
        <property type="entry name" value="Ribosomal_uL3_bac/org-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03625">
        <property type="entry name" value="L3_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11229">
        <property type="entry name" value="50S RIBOSOMAL PROTEIN L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11229:SF8">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL3M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00297">
        <property type="entry name" value="Ribosomal_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="200"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="206"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ85653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="306" mass="32861" checksum="88CDE584D0CBBB22" modified="2010-06-15" version="1">MPPRISLTPYLRQLWRSPCPLRPTQPLEPANRCRPFTTTVPNAFGNRPKRISSSGSPELLEGSKAAALERKRLATRTGVLAVKKGMTAIYKPSGRRIACTVLQLERVQVTAVKTRQKHGYWAVQVGSGWRNSNNVTRPMLGHFATAGVAPKEHLREFRVKDETGLMEPGTALSADFFVEGQYVDVRANCKGRGFAGGMKRHGFSGQPASHGNSLSHRAMGSAGQSQGGGSRVLPGKRMPGRMGGHRVTRPHAQIMEINKELGTILLFGPVPGPKGCIVAIQDTILGPPPIIPEATATPSESAIAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMB3</accession>
    <name>D5GMB3_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Exocyst complex component SEC15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00010635001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the SEC15 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430354">
        <property type="protein sequence ID" value="CAZ85656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841465.1">
        <property type="nucleotide sequence ID" value="XM_002841419.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMB3"/>
    <dbReference type="FunCoup" id="D5GMB3">
        <property type="interactions" value="519"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GMB3"/>
    <dbReference type="GeneID" id="9182351"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010635001"/>
    <dbReference type="eggNOG" id="KOG2176">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009437_2_0_1"/>
    <dbReference type="InParanoid" id="D5GMB3"/>
    <dbReference type="OMA" id="NAVMGIN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090522">
        <property type="term" value="P:vesicle tethering involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.670">
        <property type="entry name" value="Dsl1p vesicle tethering complex, Tip20p subunit, domain D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.30">
        <property type="entry name" value="Exocyst complex subunit Sec15 C-terminal domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007225">
        <property type="entry name" value="EXOC6/Sec15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046361">
        <property type="entry name" value="EXOC6/Sec15_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042045">
        <property type="entry name" value="EXOC6/Sec15_C_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048359">
        <property type="entry name" value="EXOC6_Sec15_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042044">
        <property type="entry name" value="EXOC6PINT-1/Sec15/Tip20_C_dom2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12702:SF0">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12702">
        <property type="entry name" value="SEC15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20651">
        <property type="entry name" value="EXOC6_Sec15_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04091">
        <property type="entry name" value="Sec15_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF025007">
        <property type="entry name" value="Sec15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 5" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component EXOC6/Sec15 N-terminal" evidence="7">
        <location>
            <begin position="34"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="Exocyst complex subunit EXOC6/Sec15 C-terminal" evidence="6">
        <location>
            <begin position="384"/>
            <end position="724"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR025007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ85656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="742" mass="85722" checksum="AC3B48CE2E138889" modified="2010-06-15" version="1">MMLAPSDAGYIDQLISTVGDACTLGRERQLMGDLARFSEKQEAEVERLCNNNYQDFVQSVNHLLSVRKGTVDLAKEIIELNEAIQNSTERLVEKKKALVNSREVRENIDEATKALKSCLEVLGLANRVGDLLREKKRYAALRALEELQSVRLREVAQFEIAEIIQKSVPSIKMIVKEAVMTDLNTWLYKVRENSMLLGQVAFYHTDQRRRRQKERVQGISNLRSLKINSALELVLDEREEFDVLDNEDIAIDFTPLFECLHIHEAMGQRDEFRVTYANVRRQQKDLLFSNPLTLTSEGVSCISRLLEDVCGFAIIEKSTLKKTLGFRSPVDVDYLWDSMCKKAIVMINQKVENVTKAEILLKIKNVSVLFIQTMDTWEYPVKHFDEFLLGLFDQYSECLKRDFISEFKKIAMSDDYTPMVVENRPKYDNIVKSVWYNPDKDPEIIQFPTAMPFSRIYPLCCRHIRDLLEKYYFYADEYFQNQGAIDRELQKSLDELLCEHVCGTLVEHLNSQYLGQIVQILINLEHFEYACQELEYLLAEACYANETGAIALKATERFKSEKKTAEKRIFELVNSKIDDLIGTAEYDWMATNEQSQPSEYLQQTTLFLRNNMNSTLLNLPREIQGFIYFDALNHIATSILALPLSEDVKRINKIFVHNLDMDVKYLQGFVDSLNDPMLPTVFDELRQTIDLLKSDNSDEFYNISTKMKKYANVDPINGPVLLEKLLAEDASAKKGLTGRFRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMI8</accession>
    <name>D5GMI8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00010747001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="38" mass="4514" checksum="022211D75F9E1138" modified="2010-06-15" version="1">MVNCCSAIFSFFSFFSSSFFLFIIFTQYGRSHFVNMPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>D5GML1</accession>
    <name>D5GML1_TUBMM</name>
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        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">GSTUM_00010786001</name>
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    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
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    </organism>
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        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
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                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GML1"/>
    <dbReference type="GeneID" id="9182180"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010786001"/>
    <dbReference type="HOGENOM" id="CLU_631919_0_0_1"/>
    <dbReference type="InParanoid" id="D5GML1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="178"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="316"/>
            <end position="434"/>
        </location>
    </feature>
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        <location>
            <begin position="17"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="97"/>
        </location>
    </feature>
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            <end position="200"/>
        </location>
    </feature>
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        </location>
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        </location>
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            <end position="358"/>
        </location>
    </feature>
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        <location>
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            <end position="406"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAZ85754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="434" mass="46436" checksum="96558DA581A28B1A" modified="2010-06-15" version="1">MEESGAGPPLSAAQIETSKEGRKHDSSKTARSSRVAKRSVTDGSIETEGFGRVASEWRVAKGRSKTMHNVITLSDDEGHFAQEIPEKKKKKAARQRKSATISESAKKGAKGATASTKKGKGKGKAVVNDSEDDGAFSDEAFAHHPISSPNNAVADEIAVLHPGQTPGRKRPGDQVMIAVSIESPPKPVRERGSITRKTEEINPPADVVEPDSDFMHENPQHDYESAPSSPKPVIPAKRAASGVSAGKRKKKTKPELEDEEENWDEKLKETTKKAAVKSRNAKVLDTAVIEDKAGIGDPLPKPAVRGRAAKAKVARKLPVEDEEEEAVEENLEEVEETPAALKAAARSNTTKVATTAGASETEDKRETPALAEPGADTKFSRAETETTPAPPERKSATPASASKKPAVTTPGRVPFRVGLSRKSKIPSLLKGFRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMV3</accession>
    <name>D5GMV3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00010950001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
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            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
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        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430360">
        <property type="protein sequence ID" value="CAZ85846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841655.1">
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMV3"/>
    <dbReference type="STRING" id="656061.D5GMV3"/>
    <dbReference type="GeneID" id="9183414"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010950001"/>
    <dbReference type="eggNOG" id="ENOG502QWSP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013431_1_0_1"/>
    <dbReference type="InParanoid" id="D5GMV3"/>
    <dbReference type="OMA" id="GFKKWVT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20557">
        <property type="entry name" value="CYCLIN_ScPCL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013922">
        <property type="entry name" value="Cyclin_PHO80-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615:SF118">
        <property type="entry name" value="CYCLIN, HYPOTHETICAL (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08613">
        <property type="entry name" value="Cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
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            <end position="311"/>
        </location>
    </feature>
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            <end position="362"/>
        </location>
    </feature>
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            <end position="409"/>
        </location>
    </feature>
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            <end position="488"/>
        </location>
    </feature>
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        <location>
            <begin position="55"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
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            <begin position="281"/>
            <end position="290"/>
        </location>
    </feature>
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            <end position="310"/>
        </location>
    </feature>
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        </location>
    </feature>
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            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="468"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="556" mass="61144" checksum="FF0C52F82CF559F2" modified="2010-06-15" version="1">MTNEGVGPLLPPSYGQLQQFEPQVGRGLPGPLEIFRIPSEDPYPSLLPSKRRESPPSSRRNSVVQQQQRSREEQPARRRRSSTSDASASLTVPSTINGSKANLAEFASQITCFLWFESSAVLQRVAESTAAPIPQAPLASDAIPSLGFRKWVTTILSTTQVSQNVILLALLFIYRLKKLNPTVKGKPGSEFRLLTVALMLGNKFLDDNTYTNKTWAEVSGIAVQEIHVMEVEFLSNMRYSLFVGDVEWRAWHKTLGKFASYWDKASRAPVEVSSRALAPRTPTLPSIPSSLPSPPATSSNGSSRLVSTMPTPTPALHQLSVPSQLMPAHSPITPMPQPDFQPSRKRSFDPTISMQPPTKRVTRSMAPKISITVPQYSAQIPAHAPRLPANNYPPVTSQPQQQSQSLPMMPLNQLPFPNAGGRAMSTVYSQPTPQISPVSTAPMTVSPYSAHSRTASPYSQHQTVHHSAASSPTTPGFGSQQSPTWVRNSPYRPVRRVNTLLVPPAPMSPEGMGVSWQRMHYQPLAKTRSEYKTGVVPYLQTDWPPVWSSPTPDFHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNJ0</accession>
    <name>D5GNJ0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00011310001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
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        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430365">
        <property type="protein sequence ID" value="CAZ86083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841892.1">
        <property type="nucleotide sequence ID" value="XM_002841846.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5GNJ0">
        <property type="interactions" value="60"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GNJ0"/>
    <dbReference type="GeneID" id="9186159"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011310001"/>
    <dbReference type="eggNOG" id="KOG1931">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001428_1_1_1"/>
    <dbReference type="InParanoid" id="D5GNJ0"/>
    <dbReference type="OMA" id="YEIHANP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990071">
        <property type="term" value="C:TRAPPII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034498">
        <property type="term" value="P:early endosome to Golgi transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055505">
        <property type="entry name" value="DUF7077"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056916">
        <property type="entry name" value="NTS_TR130"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045126">
        <property type="entry name" value="TRAPPC10/Trs130"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022233">
        <property type="entry name" value="TRAPPC10/Trs130_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056913">
        <property type="entry name" value="TRAPPC10/Trs130_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13251">
        <property type="entry name" value="EPILEPSY HOLOPROSENCEPHALY CANDIDATE 1/TMEM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13251:SF3">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23274">
        <property type="entry name" value="DUF7077"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24967">
        <property type="entry name" value="NTS_TR130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12584">
        <property type="entry name" value="TRAPPC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23036">
        <property type="entry name" value="TRAPPC10_1st"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24965">
        <property type="entry name" value="TRS130_4HB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="TRAPPC10/Trs130 N-terminal" evidence="6">
        <location>
            <begin position="7"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="TRAPPC10/Trs130 N-terminal" evidence="6">
        <location>
            <begin position="222"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="Trs130 NTS" evidence="8">
        <location>
            <begin position="643"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7077" evidence="7">
        <location>
            <begin position="940"/>
            <end position="1060"/>
        </location>
    </feature>
    <feature type="domain" description="TRAPPC10/Trs130 C-terminal" evidence="5">
        <location>
            <begin position="1275"/>
            <end position="1426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="147"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="351"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="512"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="154"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="518"/>
            <end position="539"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF24967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ86083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1452" mass="161843" checksum="0B093E5105B76010" modified="2010-06-15" version="1">MEVPSSTSKVTVTYWDPSGLFPLIQSDLRSRLPLRNLHWKAPARPLRSINSLHVELTPYLSPASKDPGFAGGAGHQKERRHQIPGLRNTPYLKILLLRCDDNDSYKNVARKALREWVSEHTTSASNENKQHDAFEWLIIHVVLPGTPAAQQPRSSSSASNTSGSSRWIKGNTTLLDKIRSDFNSGNNKKDRVVQIRIGPSHPALATLSPPVLVGNQIVPATESPTESEMAWLDLIAKLKTQILASFDARVSQYEEDIREKDSQRRLPGWNFCTFFVLKEGLARAFESVGLVEDALILYDELGFGLDAVVRDQRDKEKIEGDPEGVVGGNFVGWTKESIKWIEQARRRRKARLEGRKGEEQEDVDDETPVGSEKKPYRELILSNEISLFDFKTYLFARQSTLLLRLGKGNGMDGVEVPIPISGYSLHEAAAVAAGRPEEDLTRLSEVLKRGVEFVTSVGRILRADLWTSYHSSTEGEGDKEQEQKDVTWVIDNIVSSWVFAVCQQLLKQTASASLPEGPNTSTPIAATSSTPFPRRSSSLPPTPNPEATSFTQVIQTSVTGLEDFAAVRADLMLLARGVVETFGKRQGWVGKMGWFYLPEEHVKDAGMVGVDLGKGDKEVEKKEEIWGVEGIRNATLRRVVEKEEEEEFYTIFEELSEKAMKNYSIAKRTKSCERVESDLAALSFHLKDYASSVKHLEKMTKFYADQGWGLIETTLLGMYAKCLREMNRSEDYIKVLLKLLAKSSAAERARLRRRGITALDGDERAASVDTLFDGQHLEEVGVDGCVEKIITLSLEIEKEITTPMSHFWDNIVVDPYPRHLDDRDGFEVLVKMRYLLEEEMEVQKLRVRIVNTIGQVKEVWMDALGPITMKRGVVKVFARSSITIPGSYIVDKIILIANNLHFVHELITKSAPNTALSLGSTSIPAVPTVKKMKLLFYPAPRSLVVKLEAPKQIHLEHMKATELVLDPGKNNVAKGEIRLRSATAGLRLMTASVRFLEGLDVVSTGDKPGVFALKDLTQQKNVRFRIPYTSENELTELAIKVEVDYDTDNGNFFYADGLSVPVILPLAVNVQDVFKPNALFSRFQVSTAIPDVPLRVLKASLEGSRTFGACSGVGTEGSMVVFAKQPASFVYKITRKTGDDVPKDKEPQPLALTIEYRSLDEAEVQDSVVAAFSSQLEEAGLDLYSRLLVPILITRLRHRTAHDLEAAGLLNEVKLGKYDNYNWDEALESISPANGAREKAEAWLREFYEKNSIIKLDPETPPENLVLTTRSIIIPVDVPQLQVIHTVDIHILSDTNSTTLSSPYAPPMVTVGQPIPAELIIRHSRSWNSLDVKNGEVEIGNDDELEFFYEIQHSLEVWLVSGRKRAHFSAKEGQVHKFPVLLVPLKAGNLLLPNVEVKQVVPIEQGGEGASCETDYRSNGESVLVLPDVRSTTVRIDSPFGEDGFRSPASVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNN0</accession>
    <name>D5GNN0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00011382001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic RPB4 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430366">
        <property type="protein sequence ID" value="CAZ86123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841932.1">
        <property type="nucleotide sequence ID" value="XM_002841886.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNN0"/>
    <dbReference type="STRING" id="656061.D5GNN0"/>
    <dbReference type="GeneID" id="9186199"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011382001"/>
    <dbReference type="eggNOG" id="KOG2351">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110332_0_0_1"/>
    <dbReference type="InParanoid" id="D5GNN0"/>
    <dbReference type="OMA" id="HRKTQNE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030880">
        <property type="term" value="C:RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010997">
        <property type="entry name" value="HRDC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006590">
        <property type="entry name" value="RNA_pol_Rpb4/RPC9_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045222">
        <property type="entry name" value="Rpb4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005574">
        <property type="entry name" value="Rpb4/RPC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038324">
        <property type="entry name" value="Rpb4/RPC9_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21297">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03874">
        <property type="entry name" value="RNA_pol_Rpb4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00657">
        <property type="entry name" value="RPOL4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47819">
        <property type="entry name" value="HRDC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNA polymerase Rpb4/RPC9 core" evidence="4">
        <location>
            <begin position="27"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ86123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="146" mass="16582" checksum="AF531508AC60F3DC" modified="2010-06-15" version="1">MTTHTPLTRAKEQIHGDEEAGTELKLGEFQNVSSLTLSETKEVLTAVFNHRREQGKFKVPDNEVVTKTQQYLEVFSRFKQRESVQAVERILALQTDLVAFEKSQLGTLCCDTADEAKVLIPSIEGKKSDEDLQELLDEITKLRQFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>D5GP53</accession>
    <name>D5GP53_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00011707001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430373">
        <property type="protein sequence ID" value="CAZ86318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842127.1">
        <property type="nucleotide sequence ID" value="XM_002842081.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GP53"/>
    <dbReference type="FunCoup" id="D5GP53">
        <property type="interactions" value="18"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GP53"/>
    <dbReference type="GeneID" id="9186558"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011707001"/>
    <dbReference type="eggNOG" id="ENOG502SBHA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2322056_0_0_1"/>
    <dbReference type="InParanoid" id="D5GP53"/>
    <dbReference type="OMA" id="ILYHPPT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070096">
        <property type="term" value="P:mitochondrial outer membrane translocase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045040">
        <property type="term" value="P:protein insertion into mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013262">
        <property type="entry name" value="OMP_MIM1/TOM13_mt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28241">
        <property type="entry name" value="MITOCHONDRIAL IMPORT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28241:SF1">
        <property type="entry name" value="MITOCHONDRIAL IMPORT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08219">
        <property type="entry name" value="TOM13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ86318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="99" mass="10668" checksum="89841E01EA26D466" modified="2010-06-15" version="1">MSTEATAATPQPSSSTTSSPIILYKPPTIQKLLRTAAINLVLPFINGLMLGFGELFAHELAFRWGWGGTRVFPSSRVAAPGVEDKVDERGRKVKGRGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>D5GP73</accession>
    <name>D5GP73_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00011737001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430373">
        <property type="protein sequence ID" value="CAZ86338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842147.1">
        <property type="nucleotide sequence ID" value="XM_002842101.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GP73"/>
    <dbReference type="STRING" id="656061.D5GP73"/>
    <dbReference type="GeneID" id="9186578"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011737001"/>
    <dbReference type="eggNOG" id="ENOG502RZ9Z">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021915_1_0_1"/>
    <dbReference type="InParanoid" id="D5GP73"/>
    <dbReference type="OMA" id="NYQGQWP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006569">
        <property type="entry name" value="CID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12323:SF0">
        <property type="entry name" value="CALCIUM HOMEOSTASIS ENDOPLASMIC RETICULUM PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12323">
        <property type="entry name" value="SR-RELATED CTD ASSOCIATED FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04818">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51391">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CID" evidence="2">
        <location>
            <begin position="28"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="312"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="312"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="325"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="335"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="369"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="431"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="448"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="472"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="486"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="510"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="521"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ86338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="539" mass="60186" checksum="ECAE61A5775B386A" modified="2010-06-15" version="1">MASAISHQVAIARASLAAGLLRPDPTAVSRDEISSFHTLLEQALSKCSPSNIQNCKRWILRYMNSRARFAALGKFLTALSPSLSTSASTAAVGRTSARRKRVNVLYLVNDLLHHTTYNEPSDGLFAKEVEPFLADLFAAAMYPGAVKQLARLGKLLDIWAEKGYYRKSFIEELKLIVKDATSATPAARPTPSADSAKAKVSVEKPLLLPPFHGDPSLPFYDLPAANMLPHLIPNSPTPINPRLMKPIQFSTLVPNESLAAAVKDFIKSADDMFNGDDALIEDPDAIGGGGGESYYGWSKDFCHKMRQKKRAALKEKRYSRRRTHSYSPDASQQNRSASCYSRSRSPSRDRRRRSRRDSRSESPSSRSSSRSRSRSRSRSRSRSAERPSFQQPRAQPNIPPLAETHQYPPHQSGYPVPPPPHHLQYYRAQPAFPPPPPPPPQTHNWNMPHAQQQQQQQQYYQQQHQYQYNYGGPPPPPPPPPPPSAPVAHHQQQHQHPSAGAQGWQPPQGPSQDGAYTQQYEDYRSVKGREIGAKRGWKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPB3</accession>
    <name>D5GPB3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00011787001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat LST8 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430373">
        <property type="protein sequence ID" value="CAZ86378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842187.1">
        <property type="nucleotide sequence ID" value="XM_002842141.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPB3"/>
    <dbReference type="FunCoup" id="D5GPB3">
        <property type="interactions" value="559"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GPB3"/>
    <dbReference type="GeneID" id="9186618"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011787001"/>
    <dbReference type="eggNOG" id="KOG0315">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_57_5_1"/>
    <dbReference type="InParanoid" id="D5GPB3"/>
    <dbReference type="OMA" id="VQRNYKH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031931">
        <property type="term" value="C:TORC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031932">
        <property type="term" value="C:TORC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043539">
        <property type="term" value="F:protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030950">
        <property type="term" value="P:establishment or maintenance of actin cytoskeleton polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031930">
        <property type="term" value="P:mitochondria-nucleus signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032956">
        <property type="term" value="P:regulation of actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001558">
        <property type="term" value="P:regulation of cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038202">
        <property type="term" value="P:TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000339">
        <property type="entry name" value="WD-repeat protein pop3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037588">
        <property type="entry name" value="MLST8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19842">
        <property type="entry name" value="G BETA-LIKE PROTEIN GBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19842:SF0">
        <property type="entry name" value="TARGET OF RAPAMYCIN COMPLEX SUBUNIT LST8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="71"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="112"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="203"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="247"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ86378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="307" mass="34376" checksum="3149E7EA736D98E0" modified="2010-06-15" version="1">MSVIMATAGYDHTIRFWEALSGICSRTIQHSDSQVNRLCISPDKRLLAAAGHTTVKLYDIKSTNPNPLLQFEGHSNNVTGVGFHCEGKWMVTSSEDGTVKIWDTRAASVQRNYSHGSPVNDVVIHPNQGELISCDQAGNVRIWDLGENKCTHQLIPEDDVPVRSVTVATDGSMLVAGNNLGNCYVWRMIQNRDVTQLVPVTKFQAHTKYITRVLLSPDVRHLATCSADHTAKIWSVDEKKFSLENTLHGHQRWVWDCAFSADSAYLVTACSDHYARLWELSSQQIIRQYNGHHRGAVCVALNDYSER</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPQ1</accession>
    <name>D5GPQ1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00011970001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430377">
        <property type="protein sequence ID" value="CAZ86494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842303.1">
        <property type="nucleotide sequence ID" value="XM_002842257.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPQ1"/>
    <dbReference type="GeneID" id="9186726"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011970001"/>
    <dbReference type="HOGENOM" id="CLU_452124_0_0_1"/>
    <dbReference type="InParanoid" id="D5GPQ1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="219"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="343"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="385"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="546"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="219"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="394"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ86494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="604" mass="64377" checksum="E11D7767283128B7" modified="2010-06-15" version="1">MGAAQSLPSEHRPGNHTGTLPPRTKLTKPRTNSITARDHPYRNRFSVSQPRAALPDVAHHLGPEPEALGTKCTGGGGNHLVGSVTLPVYANLGINAGLQDLEVTWVGGGERSTGICAVRHSRANSSAGSFTARNERDRTTAAVGTVRRRSQLLAAPPATATRRTKCQSLTMDYGDYVDCFSQQPYAAPAELDAGRCTTPSGYSVIGAFKRGSLRIVNGAASPAPSSAPASPRISAEKEPSHQGPAISCQGSNLGLARGVHRARSLESIAPFRGVRLVPSSPRVTGQDLEDGVPESPFSFAQSPTFEPGTDTMPDLDLGPNVEYPDSVFQEEVNFYDAFESLPIYDDDNDDEPVPLSEREQASGGVPLGSRLDSRLALERPNLQDCDGCSGADSGYSSTGSVNSRTTRSRMQHDASPVVMGEGAPFRKANKGSEKVEGAEPGVRRNRSMARSMIAAIKKRCSYGDFKVVPKAEPAPELPTNKPAVKVKRSLSLLRRRRSTSTVAQDQDSTLVVPSPLPAIEPVPPLPARFVNAEPFDHLVVTNGQDVHGLPGRPGTGHGGKAITDGKERQNDCSTGVGTHKIPRRGSKITGQDYYQRFSIVETIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPR5</accession>
    <name>D5GPR5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00011988001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430377">
        <property type="protein sequence ID" value="CAZ86508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842317.1">
        <property type="nucleotide sequence ID" value="XM_002842271.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPR5"/>
    <dbReference type="GeneID" id="9183571"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011988001"/>
    <dbReference type="HOGENOM" id="CLU_2470715_0_0_1"/>
    <dbReference type="InParanoid" id="D5GPR5"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072868" evidence="3">
        <location>
            <begin position="30"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ86508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="88" mass="8878" checksum="0BAE784712BB27F3" modified="2010-06-15" version="1">MAASTSSSLFTRALLFFLLFVILAVSANAQSASETAHPVTPITNGTAEPTYTTPAVPTGTSDQPSSANTLSSTVALFGALIAAALFSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>D5G3X9</accession>
    <name>D5G3X9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00003849001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835101.1">
        <property type="nucleotide sequence ID" value="XM_002835055.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G3X9"/>
    <dbReference type="GeneID" id="9187160"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003849001"/>
    <dbReference type="HOGENOM" id="CLU_1887262_0_0_1"/>
    <dbReference type="InParanoid" id="D5G3X9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="135" mass="13739" checksum="4A7CC2B2DFCF7479" modified="2010-06-15" version="1">MCVLLDDPRQLTPCSQAWRDLIAVCSSQTCSEWAPHVSKAPPLSSCTNTPNDLPGGTASAGFSFGTFTSVARSSAAGTGSAARSTLDSASATSRKGATGPNETGKSDSGAGRVVAGGLGMGWLWEVVFLAWGAVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>D5G431</accession>
    <name>D5G431_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00003928001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835153.1">
        <property type="nucleotide sequence ID" value="XM_002835107.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G431"/>
    <dbReference type="GeneID" id="9182401"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003928001"/>
    <dbReference type="HOGENOM" id="CLU_903701_0_0_1"/>
    <dbReference type="InParanoid" id="D5G431"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="163"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="308" mass="34410" checksum="3BAC3F92A441E335" modified="2010-06-15" version="1">MGMPVWKEPVEKRDIDAVLDPRRAAVPRRSAPGRRIRSSQTLMNLLRDDLARETNTLSSIAEGRRERPYPWSELNALPGPVPDPERAGLRLTLADAEARAPSSATSGRGGIRRVRSPLPVFRSENYRLPSSASDTYTPRFPPTFPERQQEEELRRIRTPFPLPTAASSSSEGSSSSSTAPRRAGAEEGSQRRSQYFSRDESSIDETEGGRRDEFSYLTGPSARRWLGIDGLGDRERSLSPDPTHWELVANGLGFQAACDLWESDSDADGNPLAVEVMWDLPPDREEAFRIRRLLSSRNGGIDRLIERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5G442</accession>
    <name>D5G442_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00003941001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835164.1">
        <property type="nucleotide sequence ID" value="XM_002835118.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G442"/>
    <dbReference type="FunCoup" id="D5G442">
        <property type="interactions" value="277"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G442"/>
    <dbReference type="GeneID" id="9182412"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003941001"/>
    <dbReference type="eggNOG" id="KOG1438">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034315_2_0_1"/>
    <dbReference type="InParanoid" id="D5G442"/>
    <dbReference type="OMA" id="GPMTNPA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004048">
        <property type="term" value="F:anthranilate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1030.10">
        <property type="entry name" value="Nucleoside phosphorylase/phosphoribosyltransferase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.970.10">
        <property type="entry name" value="Transferase, Pyrimidine Nucleoside Phosphorylase, Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005940">
        <property type="entry name" value="Anthranilate_Pribosyl_Tfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000312">
        <property type="entry name" value="Glycosyl_Trfase_fam3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017459">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035902">
        <property type="entry name" value="Nuc_phospho_transferase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01245">
        <property type="entry name" value="trpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43285">
        <property type="entry name" value="ANTHRANILATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43285:SF2">
        <property type="entry name" value="ANTHRANILATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02885">
        <property type="entry name" value="Glycos_trans_3N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00591">
        <property type="entry name" value="Glycos_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52418">
        <property type="entry name" value="Nucleoside phosphorylase/phosphoribosyltransferase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl transferase family 3 N-terminal" evidence="4">
        <location>
            <begin position="11"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 3" evidence="3">
        <location>
            <begin position="97"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ79285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="405" mass="43136" checksum="FDB700E482F28A4B" modified="2010-06-15" version="1">MAEPKISILSLLKALSESPDTISPEDITKAITHSFNDELSPVQTGTLLTTLHFTKLDQKPEIIAAAARAMRTAGLKVDGLRADAWGDETSEGKYGGGLVDIVGTGGDGHNTFNVSTTSAILASGCGLRICKHGNKASTSASGSADILTSLGAKLMAITPSIVASIFNKPSRGNFCFLYAPVFHPAVRHVAPIRRELGHRTIFNLLGPLANPIDYSVPGGLEARIIGVGRRELGQVFAETLKILGSSKGMVVCGEEGLDEVSCERRTECWRLLEDPKTKEVMVDHFFVHPTETFGIPTHSLSKVAGGKGPDENAAILERLLKGQAQDDEKAVKDFVLVNTAALIAISGLLGPVERVPEGTVAKGAVWKNAVEKANEGIASGKAWEEWSEFVEKSRQAEVSQAWSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>D5G454</accession>
    <name>D5G454_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00003956001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835176.1">
        <property type="nucleotide sequence ID" value="XM_002835130.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G454"/>
    <dbReference type="STRING" id="656061.D5G454"/>
    <dbReference type="GeneID" id="9182424"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003956001"/>
    <dbReference type="eggNOG" id="ENOG502RZA9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_704357_0_0_1"/>
    <dbReference type="InParanoid" id="D5G454"/>
    <dbReference type="OMA" id="WETAVGY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16494">
        <property type="entry name" value="RING-CH-C4HC3_ZSWM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039903">
        <property type="entry name" value="Zswim2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21540:SF0">
        <property type="entry name" value="PHD FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21540">
        <property type="entry name" value="RING FINGER AND SWIM DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="7">
        <location>
            <begin position="210"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="RING-CH-type" evidence="8">
        <location>
            <begin position="279"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="287"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="137"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="139"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ79297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="392" mass="43083" checksum="8515269F0F8D2126" modified="2010-06-15" version="1">MGYQNDNIAVGSSPTTRATRSSSRPAPQAEAFPSLSEPQKLPEVPGGRGRPRKIAPKPAPQKQEPSKKRGRPLKRKLMDENQDDDDVVVIESASTPAPKAMGKGKQDVVPIAFAPPHPAVVAPPAVLQPFLLTQEPPQKQAKFAPSASKSSGAAPEKRAKLWRKQPPKTVSERISRCLTQRMVVLDRQRVMENDIPQEIVKIAGSTGNVYTVHIKQVPVCDCPDSSPTCKHILYVMMKVLKARSDLVYQAALLSSELREMFAKASPIHSSGESAGSSAQKPLDEDDCPICYCPFEENGREVVVYCKAQCGTNIHQSCFRQWASTRGAGTVTCVMCRQPWQGDSDPHEALAKGKVNSEGYVNVASELGMSGHRDYSTYYYPPYFGGGFRRRYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>D5G468</accession>
    <name>D5G468_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00003971001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835190.1">
        <property type="nucleotide sequence ID" value="XM_002835144.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G468"/>
    <dbReference type="STRING" id="656061.D5G468"/>
    <dbReference type="GeneID" id="9184287"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003971001"/>
    <dbReference type="HOGENOM" id="CLU_388403_0_0_1"/>
    <dbReference type="InParanoid" id="D5G468"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072506" evidence="3">
        <location>
            <begin position="29"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="166"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="277"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="304"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="384"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="410"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="458"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ79311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="711" mass="76991" checksum="509149D6D55C4817" modified="2010-06-15" version="1">MLPQNYVLPARARLEILLVLAFACNSEAASGDSPISSSSDVWATLVANIAPLLVLVGEKHVKAYFKNMCRPSHHLLFASAPIGLVTAVTTLIRLNGTPVLKRMIGRQFETRAEVLADVTSVSGGEVGLELRNMNLEQTIQPTDEDLAMFWVHGRTEGSPEEVAKTFTGWVGTLMGIGYAAANSASLYYDRRCSWEMMMSFKATGTCARWVARGHAAESRNLPELSGQCEKKVVAEKNSGDIATCTASAAVYARFTDVSLGLTASVNLDDGKLEIVRYAIIVLCSMGNVGIILAAWFRGRDPRDVGLVGAGLFVSCAGSWLTAWLVDEASEETILDMSQLSAIASGFFSDRIPEGTELSFCPKKIAISTDARPGMRGGVAKVRGWMSTVAVVAMVLAYTGLYLGLRSSDWWVSLAMLGNAAIAGIARSIVVPTALYLNSADRRLPNPMTCAIGYSANREDLSHPDKSNNGSLDIRAGSAPGQDQRVLSEPLDVGLYEAVVGEYRYKGGSRAYDNIQPYIWTALALATEMSKRGIAPLEMRDFPPRSAGIIGVEGAACIFSDIITRKGVWRQPLEVIVHPGGQSVPERVLAIFWGWYWRASAHQAQKVASIELPPDLLNRFIDLGDDDGDDDAKFGTPTDKLKEVLNSSTTPREILWMGAKLCYASCHTWDSNAFEEAGMTWFGGATHQYFRQGQILSFLDAIVAAGLTCPRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4B2</accession>
    <name>D5G4B2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00004031001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835234.1">
        <property type="nucleotide sequence ID" value="XM_002835188.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4B2"/>
    <dbReference type="STRING" id="656061.D5G4B2"/>
    <dbReference type="GeneID" id="9184980"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004031001"/>
    <dbReference type="eggNOG" id="ENOG502S7ZI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056562_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4B2"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14705">
        <property type="entry name" value="bZIP_Zip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000075">
        <property type="entry name" value="BZIP transcription factor (MetR)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13044">
        <property type="entry name" value="ACTIVATING TRANSCRIPTION FACTOR ATF 4/5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13044:SF14">
        <property type="entry name" value="CRYPTOCEPHAL, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07716">
        <property type="entry name" value="bZIP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="7">
        <location>
            <begin position="168"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="85"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="231"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="124"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="160"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="188"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ79355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="257" mass="28080" checksum="8885E83F1700059C" modified="2010-06-15" version="1">MSGHRAGGGPSYLRDLNMLPEEEIKADAEFDPSDLSLYTNTEFFEFDIGEGDGGQEGSERFTVPEEAQLKNGHLGFLNDDDFDYMPFPTLPTSESQPSTQTTVTSSHQASNFMPPTATLRAIQPSSASSDSASPSPLSPRSGKRKASIAATPPSTDGLDDLSRVAAEEDKRRRNTAASARFRVKKKQREQALERTAKEMTDRVKQLEAKIAQLEMENQWLKNLIVEKNTATAASNDSDNSRESSKDGAGTKLKNSES</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4D3</accession>
    <name>D5G4D3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00004058001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835255.1">
        <property type="nucleotide sequence ID" value="XM_002835209.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4D3"/>
    <dbReference type="STRING" id="656061.D5G4D3"/>
    <dbReference type="GeneID" id="9185001"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004058001"/>
    <dbReference type="eggNOG" id="ENOG502SQKQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1070353_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4D3"/>
    <dbReference type="OMA" id="RIDEPPE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39609:SF1">
        <property type="entry name" value="RFEG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39609">
        <property type="entry name" value="RFEG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="210"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="242"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="260" mass="28754" checksum="1C25450EED2C2796" modified="2010-06-15" version="1">MTSHGGGGYQPNYGAGYDGSSRQEMLPFFLPGEGIKRQVIQTDLPRYLGNNALSRPGWDKGVPGYWYTGYRPLTDAQVQSLKQDSQKWSSERELMSHRYPGHELPGYERSATFRDSLQTNSDGNNPNHPYPSFPPIPGPPAGSFQQYGSGQASYHHPTPYSAQPAPSGPGIPPAPNNDRSRFEYVPSAEGGKWWDRSSGQYVQSYPPPENSSTTGTRPTQNEPPTGAPDETDLPYRRPTEPNPGTEPTGYYGPTHYYPRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4F1</accession>
    <name>D5G4F1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00004081001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835273.1">
        <property type="nucleotide sequence ID" value="XM_002835227.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4F1"/>
    <dbReference type="GeneID" id="9185019"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004081001"/>
    <dbReference type="HOGENOM" id="CLU_2098609_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4F1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="116" mass="12718" checksum="340CBBF6A6DD4EA8" modified="2010-06-15" version="1">MWGSSFTSWVMLQVYQSSNRNWALRERKKTGSMCVHIPGGCRSFLYSICWGIGTPGGFPEMSRPAQSLVSPGAQNSGLQGQPLTPAHGMSLDWLAPTRWQGGWRGSKADCSTNHES</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4G5</accession>
    <name>D5G4G5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00004097001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429987">
        <property type="protein sequence ID" value="CAZ79408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842488.1">
        <property type="nucleotide sequence ID" value="XM_002842442.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4G5"/>
    <dbReference type="GeneID" id="9184037"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004097001"/>
    <dbReference type="HOGENOM" id="CLU_2147695_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4G5"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ79408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="112" mass="12549" checksum="8C3CF0C9BA791697" modified="2010-06-15" version="1">MWCRLSEATPQKYFVATRGRDVAGFVLRLSGSSSFVFIFDSALAFDGYRYCTNTMQRGSPRRARHASHASLQVSGLWHKLLSCSGCAGLLCFRRCDGLLRMVIEVMDMVMAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4I9</accession>
    <name>D5G4I9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00004133001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429987">
        <property type="protein sequence ID" value="CAZ79432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842512.1">
        <property type="nucleotide sequence ID" value="XM_002842466.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4I9"/>
    <dbReference type="STRING" id="656061.D5G4I9"/>
    <dbReference type="GeneID" id="9184061"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004133001"/>
    <dbReference type="eggNOG" id="KOG1840">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_125_15_1"/>
    <dbReference type="InParanoid" id="D5G4I9"/>
    <dbReference type="OMA" id="AQMGRRQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053137">
        <property type="entry name" value="NLR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46082:SF6">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46082">
        <property type="entry name" value="ATP/GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13374">
        <property type="entry name" value="TPR_10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ79432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="379" mass="42757" checksum="6F5564BE03628D10" modified="2010-06-15" version="1">MHRHVAQGRENALGPPHPDVLLGVVGLAVALLSEGKYEEAKLMYRHALMAMEKSLGPDHPDVLDSTNMLGETLLELERYYEAEAMWRCALASREKTLGVDHAATLTSADSLGRTLYHQRKYEEAERVFQSVQAGRKKVLGLYHPDTLLSVLRLGVTLLDRRKYAEAEIMYRHALEGMEQALGPDHPEVLDSMNTLGETLLDLKKYGEAEAMCRRASAGRENTLGPDHRDTLLSLNNLGLALRKQNKHHEAEISYRRAMMGTERTLGLDHRDTLMCIYNLAGALHKQRKYDEAEMVHRRALAGREKILGLGHRHTLRSLIKLGTVLYEKREYDEAEVIYRRAVAEAERILGPGHPVTQKAFSHLSMFLYIRAAIDPGEAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4K1</accession>
    <name>D5G4K1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00004191001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429988">
        <property type="protein sequence ID" value="CAZ79444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835287.1">
        <property type="nucleotide sequence ID" value="XM_002835241.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4K1"/>
    <dbReference type="GeneID" id="9188391"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004191001"/>
    <dbReference type="eggNOG" id="ENOG502T24I">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1066298_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4K1"/>
    <dbReference type="OMA" id="VIARKMW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="146"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="261" mass="29060" checksum="D84F3F08BC440C6B" modified="2010-06-15" version="1">MGNGMSISTFTSSLRGAMNSAGASKPVKSAIKKDICSGAGAKLKKFVRFLDRVEIVPAAPFLMFEEPSSKKQKTRESFNDPAITAGLGRAGSPNRSPNRSPNRSPNRSPNSSSKHLPATNPDNHLSPPEELYLYHHPPSPPPSWRIPPSSLTGPQPHVVTPPRVRSPSRPGDEIDLMARDNHRDSGVYMDSSEDEDEIRPVIARKMWHGEVRFEDQWGRRLIPVERKDAFRFDLWPVEEDVDNEVGDGSGWVGRFRGMSRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4S5</accession>
    <name>D5G4S5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00000079001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429990">
        <property type="protein sequence ID" value="CAZ79511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835354.1">
        <property type="nucleotide sequence ID" value="XM_002835308.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4S5"/>
    <dbReference type="GeneID" id="9188447"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000079001"/>
    <dbReference type="HOGENOM" id="CLU_3242463_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4S5"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="43" mass="5085" checksum="4D714F52BA07CF6A" modified="2010-06-15" version="1">MIRKTRISSHQQNKSQKQERPSPPQRAMQDSTQLYREVSTLLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4W5</accession>
    <name>D5G4W5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">dipeptidyl-peptidase IV</fullName>
            <ecNumber evidence="4">3.4.14.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Dipeptidyl peptidase IV</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">GSTUM_00000121001</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of an N-terminal dipeptide, Xaa-Yaa-|-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline.</text>
            <dbReference type="EC" id="3.4.14.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase S9B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.14.5" evidence="4"/>
    <dbReference type="EMBL" id="FN429992">
        <property type="protein sequence ID" value="CAZ79558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835401.1">
        <property type="nucleotide sequence ID" value="XM_002835355.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4W5"/>
    <dbReference type="GeneID" id="9183040"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000121001"/>
    <dbReference type="eggNOG" id="KOG2100">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006105_0_2_1"/>
    <dbReference type="InParanoid" id="D5G4W5"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.140.10.30">
        <property type="entry name" value="Dipeptidylpeptidase IV, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002469">
        <property type="entry name" value="Peptidase_S9B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050278">
        <property type="entry name" value="Serine_Prot_S9B/DPPIV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731:SF162">
        <property type="entry name" value="DIPEPTIDYL PEPTIDASE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731">
        <property type="entry name" value="PROTEASE FAMILY S9B,C DIPEPTIDYL-PEPTIDASE IV-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00930">
        <property type="entry name" value="DPPIV_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="10" id="KW-0720">Serine protease</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Dipeptidylpeptidase IV N-terminal" evidence="14">
        <location>
            <begin position="20"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Dipeptidylpeptidase IV N-terminal" evidence="14">
        <location>
            <begin position="137"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="13">
        <location>
            <begin position="373"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAZ79558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="443" mass="50196" checksum="93A4232F209CAEB5" modified="2010-06-15" version="1">MECGGEDLDSPRRGTEWRWDDLFIWRDGVSTRVTSDGGENTFNKIMEDKYSLWFSPDGEYLAFLRYNETGIPASILPRYMAGQEVAPPHPDEKIFKYTKPGDKLPIVTFHLLEVNNPSVARKVDIQTYTQIIWSLPSGNSRVVRGRNATDGWLDRFLAKLFWLDVPGLSTPSYLDILDHSGWAHIYLYPVAGGEPIALTSGNWEVTTIYSIDIKRGLVYYQSTERDSTERHIFSVSLDGKTKNSLVDITKEGYYDASFFPGGGYYILSYRGPNLPWQELRSTSPDTPIRVIDDNASLKKKLSEYDLPKISWSTLKHPDGYELNFMERLPPKFRKGVKYPVLFYIYGGPGSQETAKTFRQTIDIYSYLGSDPELEYIVVTVDNRGTGFKGRKFRSLVTGQLGKLEAEDQIAIWGWSYGGYLTGKVLELDSGVFSLGIMTAPVTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>D5G519</accession>
    <name>D5G519_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00000326001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429995">
        <property type="protein sequence ID" value="CAZ79612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835455.1">
        <property type="nucleotide sequence ID" value="XM_002835409.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G519"/>
    <dbReference type="GeneID" id="9186310"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000326001"/>
    <dbReference type="eggNOG" id="ENOG502QUBE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047729_3_2_1"/>
    <dbReference type="InParanoid" id="D5G519"/>
    <dbReference type="OMA" id="VQMAQTN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021476">
        <property type="entry name" value="Egh16-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34618:SF4">
        <property type="entry name" value="CAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34618">
        <property type="entry name" value="SURFACE PROTEIN MAS1, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11327">
        <property type="entry name" value="Egh16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003071981" evidence="1">
        <location>
            <begin position="23"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="215" mass="22477" checksum="1B738FFA709725BB" modified="2010-06-15" version="1">MHMHYFLDIVLFTTTLITVTSAHGVITSATGDAGGQGTALAVDAATPRDGTRRRPFQQDTTVFEEEDDDEGVARTGCGMTLQAGEINIPTAMQSVITQNGGLPQVSPGGELTMTLHQVNGDGAGPYECMIDQTGTGASFTPMTVTQNVPGQDGRDRDGSETDFPLVAQMPANMACTGTAGAMTGICMVRCQNPANAGPFGGCTNFLYPPLLPREN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5G529</accession>
    <name>D5G529_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00000287001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429996">
        <property type="protein sequence ID" value="CAZ79660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835503.1">
        <property type="nucleotide sequence ID" value="XM_002835457.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G529"/>
    <dbReference type="GeneID" id="9186358"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000287001"/>
    <dbReference type="HOGENOM" id="CLU_2924390_0_0_1"/>
    <dbReference type="InParanoid" id="D5G529"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="61" mass="7245" checksum="FE75E138D3F28098" modified="2010-06-15" version="1">MTSRWKRKEVQDHSASKPASPPIPHQKRNPPFPWRILHSFPKRHNLVYAVRKEVNKFSVAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>D5G539</accession>
    <name>D5G539_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00000239001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429996">
        <property type="protein sequence ID" value="CAZ79624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G539"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000239001"/>
    <dbReference type="HOGENOM" id="CLU_2795779_0_0_1"/>
    <dbReference type="InParanoid" id="D5G539"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ79624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="68" mass="7897" checksum="5A9175095E3EAFAC" modified="2010-06-15" version="1">MRKERPKQHPSEQTKGGKNRIRKASRQPIRNGVAHFIQKLFTDFSFFLFFSFLSVAAGQNEMGCLFSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5X0</accession>
    <name>D5G5X0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TBP-associated factor 6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Transcription initiation factor TFIID subunit 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00001607001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF6 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430002">
        <property type="protein sequence ID" value="CAZ79913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835756.1">
        <property type="nucleotide sequence ID" value="XM_002835710.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5X0"/>
    <dbReference type="FunCoup" id="D5G5X0">
        <property type="interactions" value="1012"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G5X0"/>
    <dbReference type="GeneID" id="9187591"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001607001"/>
    <dbReference type="eggNOG" id="KOG2549">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021711_3_0_1"/>
    <dbReference type="InParanoid" id="D5G5X0"/>
    <dbReference type="OMA" id="YFVQFIA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046695">
        <property type="term" value="C:SLIK (SAGA-like) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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        <source>
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    <sequence length="472" mass="51922" checksum="4ED7AD3AA3A34B87" modified="2010-06-15" version="1">MSLWNGDTIKDVSESVGVANLNEDVAKNLAMDVEYRIHQVLQEALKFMRHARRTTLGTQDISNALRVLDVEPLYGYESTRPLRFGEASLGQLQPIFYVEDDEVDFEKLINAPLPKVPRDVTFTGHWLAIEGVQPAIPQNPTPSEAARLSETTPKGASSNTTLSAASTLNPTTNETVTIKPLVKHVLSKELQLYFERISTSITDESTTDTIRNAALASLRKDPGLHQLLPYFVQFISEKTTHGLRSLFTLTQMMSLTHALLENDSFFIEPYVSSLIPPILTCLIGKHLGSSSSDPHSQTPAHYALRDLSASLLKLVCKRFGDSSHTLKPRLTRTCLKHFLDPAKPLPTHYGSIIGLAAIGGREAVRVLILPNTKLYEKVIRLEIEDEGPRKSEAEMCLSALVGVIKQLEEEEEEGAGGEKGKNGVVPIIPGANADEVKVQLIGILGELVGEEVWKLRREALVKAVIEAGGMRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5Y7</accession>
    <name>D5G5Y7_TUBMM</name>
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        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
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    <organism evidence="1 2">
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        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
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            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="InParanoid" id="D5G5Y7"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="EMBL" id="CAZ79930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="15" mass="1661" checksum="9F033D6096F4FF2C" modified="2010-06-15" version="1">MTENGGQSRSSLTHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6A5</accession>
    <name>D5G6A5_TUBMM</name>
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        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00001677001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
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            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="CAZ80048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835891.1">
        <property type="nucleotide sequence ID" value="XM_002835845.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6A5"/>
    <dbReference type="GeneID" id="9187719"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001677001"/>
    <dbReference type="HOGENOM" id="CLU_3377393_0_0_1"/>
    <dbReference type="InParanoid" id="D5G6A5"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072540" evidence="1">
        <location>
            <begin position="23"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="34" mass="4078" checksum="F5F91D3C9414A0F1" modified="2010-06-15" version="1">MGWVFGVFPLLFLLLRVHSVEQANEMIETIQWSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6G2</accession>
    <name>D5G6G2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00004468001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
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            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430009">
        <property type="protein sequence ID" value="CAZ80105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835948.1">
        <property type="nucleotide sequence ID" value="XM_002835902.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6G2"/>
    <dbReference type="GeneID" id="9181190"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004468001"/>
    <dbReference type="HOGENOM" id="CLU_977241_0_0_1"/>
    <dbReference type="InParanoid" id="D5G6G2"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="242"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="257"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="285" mass="33069" checksum="B09840268EF54302" modified="2010-06-15" version="1">MDRPTNDIWRTRGCNSWYKSLYVHPPPPSSPYLLLSYTHISNTPTAFLAVLLCRRHPYDNKSRDGTKQDWYLWDPTSKLLPPSTSTTTSPHLPHRRRKTILSVWRDQSKFQPAPGTILALRNLTIDKHWNDTLADQSWKEILDDEHWDERIGPPVKMMKVGRGTGHGNINAFPTTPVEWFLLDASGVEGYRAVKEWWESVGRREWEAEVRVDERLKREIGARRESMRRDREAVLARQRICDGAGGGGEGLKRKRESEEDYAGTDDLETERLMMEELKRDRNFGNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6Y3</accession>
    <name>D5G6Y3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00002382001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430019">
        <property type="protein sequence ID" value="CAZ80276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842541.1">
        <property type="nucleotide sequence ID" value="XM_002842495.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6Y3"/>
    <dbReference type="STRING" id="656061.D5G6Y3"/>
    <dbReference type="GeneID" id="9184090"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002382001"/>
    <dbReference type="eggNOG" id="ENOG502S3XI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039527_0_0_1"/>
    <dbReference type="InParanoid" id="D5G6Y3"/>
    <dbReference type="OMA" id="DHHWDEM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="209"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="305"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="225"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="305"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="322"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="336" mass="36498" checksum="2D7C271D34A0C5E6" modified="2010-06-15" version="1">MALCGPSTALGSLQKHVSADKTLQQDRVQRQGSFPNAFRTQNTDPRLEHEFSQFSSSVAAANLEIATAHHHQQQHQHLGNVDASWASDFRRLHVGAGPPLRLPHHQQQQQWGGGWHEEFLSHPTTAAAGIYTTTPEHVHPDPQQLAIFSGTNTSAGFPRYTPISQNPYHSPAATTAPTTITSVEEDLINEAFEKAFESAGAQLAEPIATTPQPTVAPPPQAVVEEPPKQEESNQDKDCDDLSRTAGNLLTSLQNNQSQKFRESNFLALMRRLRDREVKVEGDGLVEISDDRGRRSDGSVGVGVAIGGGESWGDGGGGEGDGVGDREGFLEHSWWDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5G710</accession>
    <name>D5G710_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">mRNA 3'-end-processing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">GSTUM_00004539001</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 12">Component of the cleavage factor I (CF I) involved in pre-mRNA 3'-end processing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the CPSF4/YTH1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430020">
        <property type="protein sequence ID" value="CAZ80303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836112.1">
        <property type="nucleotide sequence ID" value="XM_002836066.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G710"/>
    <dbReference type="FunCoup" id="D5G710">
        <property type="interactions" value="74"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G710"/>
    <dbReference type="GeneID" id="9182494"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004539001"/>
    <dbReference type="eggNOG" id="KOG1040">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024513_1_1_1"/>
    <dbReference type="InParanoid" id="D5G710"/>
    <dbReference type="OMA" id="AHARWIK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045348">
        <property type="entry name" value="CPSF4/Yth1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23102:SF24">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23102">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR SUBUNIT 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14608">
        <property type="entry name" value="zf-CCCH_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 12" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 11 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 11 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="14">
        <location>
            <begin position="47"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="14">
        <location>
            <begin position="76"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="14">
        <location>
            <begin position="101"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="14">
        <location>
            <begin position="130"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="14">
        <location>
            <begin position="159"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="11">
        <location>
            <begin position="47"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="11">
        <location>
            <begin position="76"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="11">
        <location>
            <begin position="101"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="11">
        <location>
            <begin position="130"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="11">
        <location>
            <begin position="159"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="179"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="194"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU369008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAZ80303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="236" mass="26825" checksum="6F94A23620C26BBD" modified="2010-06-15" version="1">MATATTTAAASPDASLAEKILNPQSQQFGNRFAFHEFLRKEYRFGLDPNRRTCPLFVQGHCPMGNSCPDKHAVSSSFNNLVCKHWLRSLCKKGEACEFLHERKMPECNFFVRNGYCSNGEECLYLHVDPDSKIPPCPHFENGFCPLGPTCSKKHVRKQICKFYLAGFCPDGRSNCKEGTHPKWKTGLPAPTVKKPREEVEKERDGDREGGGEGFRGRGSFERRRFYGGRGRRDRDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5G735</accession>
    <name>D5G735_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00002323001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430021">
        <property type="protein sequence ID" value="CAZ80328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836137.1">
        <property type="nucleotide sequence ID" value="XM_002836091.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G735"/>
    <dbReference type="GeneID" id="9182519"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002323001"/>
    <dbReference type="HOGENOM" id="CLU_1230690_0_0_1"/>
    <dbReference type="InParanoid" id="D5G735"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ80328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="225" mass="25654" checksum="45F19DF56C10CF79" modified="2010-06-15" version="1">MPRDRELTDPQKTAEYLRTAANQFNLFPNIPVHQQENLPSELLQRMLNNGQLFATDLQTIAQRLGSPPLLTGAPPPAIEYQVETSNQEHNPIMEQLCNFRILAQQFCVVADQIELVPAPYTVRIDGRPRVMDLRMRIMNYLKILQADLQLLTQCLSGDPQLLQISPEIVHGQDQNSNTQYNQFSPHSPDIPPMKTQLKEQVLTLSHNKDNGGELTENLRLPKWSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5G783</accession>
    <name>D5G783_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nuclear pore protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00002509001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus envelope</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
            <location evidence="4">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the nucleoporin interacting component (NIC) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430022">
        <property type="protein sequence ID" value="CAZ80376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836185.1">
        <property type="nucleotide sequence ID" value="XM_002836139.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G783"/>
    <dbReference type="FunCoup" id="D5G783">
        <property type="interactions" value="1063"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G783"/>
    <dbReference type="GeneID" id="9185274"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002509001"/>
    <dbReference type="eggNOG" id="KOG2168">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011846_0_0_1"/>
    <dbReference type="InParanoid" id="D5G783"/>
    <dbReference type="OMA" id="LLMCGQF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007231">
        <property type="entry name" value="Nucleoporin_int_Nup93/Nic96"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11225:SF4">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP93"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11225">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP93 NUCLEOPORIN NUP93 DEAD EYE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04097">
        <property type="entry name" value="Nic96"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="4" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0811">Translocation</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ80376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="964" mass="107963" checksum="6333C81D4C8399A4" modified="2010-06-15" version="1">MTEPVANFPTSTLFSSLLEQSRKLRQGQDSGELPPIQLGLSEIERRAKELRRAPGGQRQTDARAHYLLAAGGINTDEVLRDLDSIDFHHAYEPSSLRADTDLDTYLRNRKEQNVMSSIEDGMRRTGRDFDAFVAKNVTMEWEAQKQRIFEHFGLAPRSAVAAGGSPADLRASGFGGGLSAFGASSFGRSRLNSSFGPGRGLQTSVWSKTTLGNSVLGRSTSAAASSGHQGTLFGDIDPGRQLELSRQVQVRQQHYTLAIRRMNEVRLESGNAARGVFPVMKTFGDITGKSGTDLLTVQLHDSWMLLSYITGESEVHDANPFSGPRERGFVKDYVIDTASDSQEASRIRKHVQRGSRRFLEKQYRDVVESAIAENPQIAKVGGIPSVFHKFRGYINIKLATNKDIPEWDIKNFDFVADIPCWALIFYLIRAGYLEEADEFVRQNQQSFQKLDRSFPTYLHAYCSAGDERRLPRNLLDRIQAEYNNRVKHSEDTKDIFKPAVYKIIGRCELAKRSVPTVMPTAEDWMWLQLVLAREIDRSTDPAHEVFTMQDLQKGVIQFGAKHFGTKGSNVGLYFQMLLMSGLFEDAVHYLYSFQFVDGVHFAIALSYYGLLRPTANPAKADTDLLTLGPKGEKQINFPRLIGYYTRDFRTPNAEEAVDYLCLICLNGDLPSPAGDQHLTICHDALRELVLETREFTKLLGDVHADGTRQKGAIERRMKLIKLDDQREYLRTITEQAAGQANDDGRASDAILLYHLAEDFETVVEIINKSLSDSIASEDVMLDFSHQAGTSALPNADSSLTSVDDPVQLAKRMLGMCANDAAIYSKISIRSREAAEVLLSIAEAKKRYREGKWELCLQIIQDVDVIPIEKDADVGSIRRRAQNFGTLHDTVASNVGILLKMTVECCSRLSAELRDSMYADASRSQKLIELKTRAKNVMIYAGMIQYKLPAHVYEFINSRDMTEGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7F2</accession>
    <name>D5G7F2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00002536001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430026">
        <property type="protein sequence ID" value="CAZ80445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836254.1">
        <property type="nucleotide sequence ID" value="XM_002836208.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7F2"/>
    <dbReference type="GeneID" id="9188469"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002536001"/>
    <dbReference type="HOGENOM" id="CLU_2428659_0_0_1"/>
    <dbReference type="InParanoid" id="D5G7F2"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ80445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="91" mass="10058" checksum="DF0F576BDC1685C9" modified="2010-06-15" version="1">MCRRREKGFLGEELLETQAIQAGALLGAWTEKCDGGGRVSYVVWETGVGVGWWDVLEELCMRELLLWDLRSVRGQGIMHQGSHGFGGYSGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7J9</accession>
    <name>D5G7J9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00002607001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430029">
        <property type="protein sequence ID" value="CAZ80492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836301.1">
        <property type="nucleotide sequence ID" value="XM_002836255.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7J9"/>
    <dbReference type="STRING" id="656061.D5G7J9"/>
    <dbReference type="GeneID" id="9186971"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002607001"/>
    <dbReference type="HOGENOM" id="CLU_792714_0_0_1"/>
    <dbReference type="InParanoid" id="D5G7J9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="291"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="372" mass="42791" checksum="697D3D11F88CFD71" modified="2010-06-15" version="1">MRKPDITSHFRGGMEELKTADGITLYAHKSILDLGTEPCPDTPWDSFTTSTVERFLEHCYQGDYSPPKPVKLPLATPTSSVVDGDGHADGVFHTAAGRLPHCQSANGLVVAQHGQHFCCSCVAIQDDDTEEDEANKDDDAEGSDTEYGDDDKDDSGSLGHPNGLDYSEVFFAHAELYILSQTQGRSLLTALCLDRLRTALGEAAKAPVRPRFATNLGRLLFYVYEHGNVRLIDDNQAHDLQNLASSYAAMHIEEMKEECSSLMRHGGKMAEDLMKEVADRAINLNSVLKKEAEKREREEREKEQREKEQREKEQREKEQREKEQREKEQREKEQKKREEREQREREEREQREREEREKREEREREEAEKAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7T0</accession>
    <name>D5G7T0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="13">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">GSTUM_00004710001</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the helicase family. RAD25/XPB subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="13"/>
    <dbReference type="EMBL" id="FN430031">
        <property type="protein sequence ID" value="CAZ80573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836382.1">
        <property type="nucleotide sequence ID" value="XM_002836336.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7T0"/>
    <dbReference type="FunCoup" id="D5G7T0">
        <property type="interactions" value="960"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G7T0"/>
    <dbReference type="GeneID" id="9181392"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004710001"/>
    <dbReference type="eggNOG" id="KOG1123">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008213_0_0_1"/>
    <dbReference type="InParanoid" id="D5G7T0"/>
    <dbReference type="OMA" id="RCQEIDY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000112">
        <property type="term" value="C:nucleotide-excision repair factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18029">
        <property type="entry name" value="DEXHc_XPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18789">
        <property type="entry name" value="SF2_C_XPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000077">
        <property type="entry name" value="Probable DNA repair helicase RAD25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000117">
        <property type="entry name" value="Putative DNA repair helicase rad25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050615">
        <property type="entry name" value="ATP-dep_DNA_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032438">
        <property type="entry name" value="ERCC3_RAD25_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001161">
        <property type="entry name" value="XPB/Ssl2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032830">
        <property type="entry name" value="XPB/Ssl2_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00603">
        <property type="entry name" value="rad25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11274:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION AND DNA REPAIR FACTOR IIH HELICASE SUBUNIT XPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11274">
        <property type="entry name" value="RAD25/XP-B DNA REPAIR HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16203">
        <property type="entry name" value="ERCC3_RAD25_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13625">
        <property type="entry name" value="Helicase_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00851">
        <property type="entry name" value="XRODRMPGMNTB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="16">
        <location>
            <begin position="346"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="17">
        <location>
            <begin position="563"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="798"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="812"/>
            <end position="826"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAZ80573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="826" mass="92292" checksum="EE7578A8AAD918FC" modified="2010-06-15" version="1">MPPKRKAGATGGAAAVATTSAATKRSRPATPAVDVDSSDEYSDDEEIENIEEGRAISKAVNAFSLDLAPKGSDVIAKRDSVSYIFSKQDFSYLKMKLDHESRPLWINPESGVIVLESFSPLAGQAQDFLTTIAEPLSRPTYLHEYKLTPHSLYAAVSVGLDPNDIISVMDRLSKIPVPPKIIDFIKKCTSSYGKVKLVLKENRYFVESSDPAMLQRLLQDPVIGPLRVQSEGGSGITKEKAPQMGALVIPGTRNAAGAASVSNPQGGRPAEVAGDAVEDLFNSDAVHSFEIPANDVETVQKQCLDLGYPVLEEYDFRNDRKNANLDMDLKPQAQIRSYQEKSLSKMFGNGRAKSGIIVLPCGAGKTLVGITAACTIKKSVVVLATSSMSVVQWRQEFIKWTNINPDAIAIFTSENKEKFRGDAGIIVSTYSMVTNSRNRSHDSQKMMDFLQSREWGLLILDEVHVVPAQIFRKVVHSIATHSKLGLTATLLREDDKITDLNFLIGPKLYEANWMELAEQGHIAKVQCAEVWCPMTPEFYSEYLQEPDARKKVLHYIMNPRKFQATQFLIDYHEKRGDKIIVFSDNVYALRAYALKLNKAFIYGDTPQQERLRILENFQLNDQVNTIFLSKIGDTSLDLPEATCLIQISSHYGSRRQEAQRLGRILRAKRRNDEGFNAFFYSLVSKDTAEMYYSSKRQAFLVDQGYAFKVITHLQGIEQLPGLAYATVAERKELLAEVMLQNSEAGQQEGDDFDGAKLSYNAGRKGARYRRTTGRLSELAGGDDMAYIEYNRSKNKELKGKGQSSAFFKKMHRENEKRRKEQNALKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7U6</accession>
    <name>D5G7U6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00002657001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430032">
        <property type="protein sequence ID" value="CAZ80589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836398.1">
        <property type="nucleotide sequence ID" value="XM_002836352.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7U6"/>
    <dbReference type="GeneID" id="9181408"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002657001"/>
    <dbReference type="eggNOG" id="ENOG502SGUN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027515_0_0_1"/>
    <dbReference type="InParanoid" id="D5G7U6"/>
    <dbReference type="OMA" id="HGCNCYS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035197">
        <property type="term" value="F:siRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031048">
        <property type="term" value="P:regulatory ncRNA-mediated heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048263">
        <property type="entry name" value="Arb2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053858">
        <property type="entry name" value="Arb2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21357">
        <property type="entry name" value="FAM172 FAMILY PROTEIN HOMOLOG CG10038"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21357:SF4">
        <property type="entry name" value="FAM172 FAMILY PROTEIN HOMOLOG CG10038"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22749">
        <property type="entry name" value="Arb2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arb2" evidence="1">
        <location>
            <begin position="16"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="397" mass="44507" checksum="B4EA2583824E299E" modified="2010-06-15" version="1">MFRRKKTGRNPVEIEFPHDLKELGLFINEKDQLRQIEKPNEGFDYFAHGRHHGYMYAANGGHGVSRERLNERRREAVDACLRSIVITRLEGLGLTKLLLPLGASAEEPHVPIMVSQNLQTCDKVLVFSPDSNSGALGIWCMRSIEEGALNDGSMIDAVKRALESGYGVVIMNAGQLLWDYTTKSPMGFGTWRATKDKKTPINAFKNTAPSNRDPEEHVSYVFKHILDKVVPSHAEIDIMACGMAAYYIVMFLNNNWDNWKCRVFALVMAESTHCLSNITNIELQQFLMQRARNYIVHSKPRESVILDPRFGCATFSSSAKWPSAIVPSCLDLFMGYLNKAHADPENCNPDVPVIVGDVPDHDEWMNAMKNVDQNTALHVLDSDSFSESTQEGTAAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7Y2</accession>
    <name>D5G7Y2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00002630001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PHAF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430033">
        <property type="protein sequence ID" value="CAZ80625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836434.1">
        <property type="nucleotide sequence ID" value="XM_002836388.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7Y2"/>
    <dbReference type="STRING" id="656061.D5G7Y2"/>
    <dbReference type="GeneID" id="9181444"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002630001"/>
    <dbReference type="eggNOG" id="KOG2819">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032056_3_0_1"/>
    <dbReference type="InParanoid" id="D5G7Y2"/>
    <dbReference type="OMA" id="YRKNDQK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005373">
        <property type="entry name" value="PHAF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039156">
        <property type="entry name" value="PHAF1/BROMI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13465:SF2">
        <property type="entry name" value="PHAGOSOME ASSEMBLY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13465">
        <property type="entry name" value="UPF0183 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03676">
        <property type="entry name" value="PHAF1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="268"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="395"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="284"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00024339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ80625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="468" mass="51407" checksum="2DF28D2A5B7D49B5" modified="2010-06-15" version="1">MASSIEGTAQIQPSKALGFLVLGSSVYDVLSRLRAQHRIFPSISVIYDSAAPLASPIVLMLDSNGLRLRFDGPDQRLRLIEVLDFQKSRLSYNSGELTRPGIGAGGPTFRSIYNKLFGPTYPGEYMEGEGVYVLSYPGVAFSFPIGREAWKEGADFVSVLSSPDAQPASSMAIFAGSSWLEARTGLFTRPVQNPRSPLVSGASARLSSANDEVEFIKFRDENKVEITRRHNQPFWITLHSTTPQDLITELGPPSAIYRKHDHRLSIHRTRSLSGGVSGRGHQDDTDDTEPEDAQSDDEEDVSDVSATSNSDYFYNYFHHGMDIFISATRSSSHPVATKIIIHGNVPGSFEFQGYRRARWSIELAASSKMAKELRLHSEMGFDEALPLLREKFGEGQRPMPLNRGSDSPSSSCELLGGWEDNESAADKVDVKGGPGGSESTFGNTELYGFPGFIFEVLRNKAISSLTVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7Z5</accession>
    <name>D5G7Z5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00002743001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430035">
        <property type="protein sequence ID" value="CAZ80638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836447.1">
        <property type="nucleotide sequence ID" value="XM_002836401.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5G7Z5">
        <property type="interactions" value="455"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G7Z5"/>
    <dbReference type="GeneID" id="9184111"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002743001"/>
    <dbReference type="eggNOG" id="KOG0379">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002697_1_0_1"/>
    <dbReference type="InParanoid" id="D5G7Z5"/>
    <dbReference type="OMA" id="CIGYIPA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051285">
        <property type="term" value="C:cell cortex of cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061245">
        <property type="term" value="P:establishment or maintenance of bipolar cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000049">
        <property type="entry name" value="Cell polarity protein (Tea1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23244">
        <property type="entry name" value="KELCH REPEAT DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23244:SF456">
        <property type="entry name" value="MULTIPLE EPIDERMAL GROWTH FACTOR-LIKE DOMAINS PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="444"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1126"/>
            <end position="1149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1234"/>
            <end position="1253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1449"/>
            <end position="1494"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="859"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="943"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="1071"/>
            <end position="1098"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="31"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="43"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="93"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="454"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="496"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="509"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="556"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="595"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="616"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="667"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1136"/>
            <end position="1149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1234"/>
            <end position="1244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1449"/>
            <end position="1468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1475"/>
            <end position="1486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ80638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1494" mass="161979" checksum="3DBD841B6F9DAC5C" modified="2010-06-15" version="1">MAFLFGKKKGAHGHREGTPNGPIPPTAPGALGNGAPGGPGGPTPSSSVNNSLNSLAGSIGATSSPDQRPVGAPGGPPRLQQALQPGPLENQAPGPQQNQSPSGVNPANVALYPWSQRRLTLSSSHPSPFPRYGHAANGVAGKDGDIYIMGGLIRSQTVRGDLWMIEGGGTQLAAYPVMTTSEGPGPRVGHASLLVGNAFIVFGGDTKLDPQDVLDETLYLLNTSTRQWSRANPNGPRPAGRYGHTLNILGSKLYIFGGQVDGFFFNDLVAFDLNTLQSAGSGWEVLVPAREAGADMPASRTNHTIVTWADKLYLFGGTNGLAWFNDVWSYDPRSNSWSQLDCIGYIPSPREGHAAALVGDVMYIFGGRTSEGEDLGDLAAFRISSRRWYTFQNMGPSPSRRSGHSMTTCGQKIIVLGGEPSMPSRNQEELQFIYVLDTAKIRYPTDHQQGSPTDRNHAPTDKRIQGLRNGSVDGPGPGPGDPRSRGAKQGPRESVTTSGSPGSTGSTPLASNNTSGSSVNSRLPRASISQAPVGPPPTQQAPAPKANGVPPGPRSRTPTGSSGLSMNINNNKPKDNASSIQPQPRDGSAGAVNGRTPTAQQRKGSTDVNTDKDRSPSSPAPNQYPSSQLNNLANGNSAVMSPHNPSGTSAPGGVPAVTESGDPPTKALEKKGSTSGQEMERLKHQNDWYSSELALARRAGYTPQTNGSAILDERANESLAEDDRPFIEAMLVLKGELSRVQGSVDTQAGLAAKRIQDIERQRDVAVQEAVYAKAKLAALGGNNATPTGDKSAVDIANMDAEKMADMSRKLASSLAAQAELSAKVEVLAAEITAERKARHLADETAAVAQSRVTELDDFRNRAASEMESLRAELLGAERAFRDEATSRTEATAEAKLLRIDQEELSMKLKEALEDNSGYQGLMGELRKAMEASSERSTTLARQLEEERMVKEGLERKLAQVKSEYEEKVADLTSANTRLRDTEELLEKYAEEARAASAAMAAGLQKVSEREIMGGTGAADERVRVLQEQVASANSLLAKSKTQADETGENLAQAMQRVAGLEFQQGQASKDAIALRRRMAEVLDEVRRLKQENAESSARLLDRQLEVDSITTKHNALKEILAERSSMAGYDKRRSHSLQSPSPSGTATPEQINRLRELETRLEESLRAHRETKSTAEMQAQEVEKHFREKLEQLENDYQSAVHYVKGTEKLLKRMKDELAKNKSQNARLHLELEEAQRKNSERSRQGSISDIGAESWESERASLKKEIDDLRTKVRESATALDKQIRESKETLDTLREERDQFRIGHNQLQLQLMDVSQQLGETRQIVEKLEIENNQLERRAQDAEERVSFLLDRVEDSVDAYRRSTRLEGANGSIETGSARSSFYGAGPDARNSVALDLFATELDALRTRWENTNKNYRLSSTFDFEKTPTSTEGGELSNSLAIWRQKLQQEESDAAKRSDSRGDEARSPVSRSQQQLNHLQAQQQPVIAGGVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5G802</accession>
    <name>D5G802_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00002751001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430035">
        <property type="protein sequence ID" value="CAZ80645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836454.1">
        <property type="nucleotide sequence ID" value="XM_002836408.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G802"/>
    <dbReference type="STRING" id="656061.D5G802"/>
    <dbReference type="GeneID" id="9184118"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002751001"/>
    <dbReference type="eggNOG" id="ENOG502SBDU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_636456_0_0_1"/>
    <dbReference type="InParanoid" id="D5G802"/>
    <dbReference type="OMA" id="GVKGPSN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013658">
        <property type="entry name" value="SGL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051262">
        <property type="entry name" value="SMP-30/CGR1_Lactonase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47572:SF4">
        <property type="entry name" value="LACTONASE DRP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47572">
        <property type="entry name" value="LIPOPROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08450">
        <property type="entry name" value="SGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072238" evidence="3">
        <location>
            <begin position="18"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="SMP-30/Gluconolactonase/LRE-like region" evidence="4">
        <location>
            <begin position="129"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="66"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="348"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="361"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="383"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ80645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="431" mass="46938" checksum="DB56F05BFC4A7AC5" modified="2010-06-15" version="1">MKSAISFALLAAATVHGAVHNSTVSATSVADKFPIINFMGPTIEGASVNRKGELFAVNKTHFLSLKSGTSDPKNPKLTGPGDKTSHFASSRFTRTQDVLIGDAVGHSIWQQDYNSSDLKRRWNGRFARRAYTPFLINDKLLQPNDFTINGAETYLYMSGMNYTATTVAGEHGDVWYYNIKTGAYKQVPKEVMRGAGIHRCNGIDLSPDDKYLYVTSAQNINDVVTSTKVYKFVIDPATGEPKNPTTEVDLYQVLSNAGVKARERGMDPDGMRMDVEGNLFITLNAIPGVLRWNTKAPGDGTYIPLTTVFAPSNLEFGGEDGKTLYIVGRCQGLEKACVDAYKHDKPGRAITNLRGTTPPKTNSNTGTGSGNSGNSTSPGTEYPNVSNPPNTGSGSPTDAEKEMMRMKLLEWIKIIEKYIATWLKDGNPVPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5G879</accession>
    <name>D5G879_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00002921001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430041">
        <property type="protein sequence ID" value="CAZ80722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836531.1">
        <property type="nucleotide sequence ID" value="XM_002836485.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G879"/>
    <dbReference type="FunCoup" id="D5G879">
        <property type="interactions" value="101"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G879"/>
    <dbReference type="GeneID" id="9188324"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002921001"/>
    <dbReference type="eggNOG" id="KOG2354">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_642811_0_0_1"/>
    <dbReference type="InParanoid" id="D5G879"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006886">
        <property type="entry name" value="RNA_pol_III_Rpc5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12069:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12069">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES III 80 KDA POLYPEPTIDE RNA POLYMERASE III SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04801">
        <property type="entry name" value="RPC5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="207"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="26"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="49"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="211"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="427" mass="47019" checksum="64B5275E22ADCF26" modified="2010-06-15" version="1">MPPKRKPNKSAGRPRPAAVATKPEEPAQSAEVAATAPTAETPPLPPPNSSENPLFLPGYSDSSSDDDNDNNSNNGSIEIVSPPPPPPTDPLLQPTPKPTTAQPPHPPPRNEDDDTSGGEDTTDEDPVVQTYDIQITTSQLRDLYIFQYPVRPREQPYTKARNACPIEARFKPKSGLVEVDIPVNVQVNYDEEKGRVWGDVMRKAEKEAGKVQGGGGRSGRIGSVGGGGKKRKILRGEDDEGEEEEEEEDLMYMDLAAAVKARRILNRQTLGSKIQPDGTKYMAGVFKDDKLYLTPITSTLQLRPQFRHIDTQIDLERAANKALHPVKQEARAIQLSVKNSEDPSSQLSSSMKAVRLAEEENWTKLHWVDQDTDDAWGVYEDVCLTAEEVGEEGKVRDGLLKCVTTKKEYIEWLRAGKPIVKKEGGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8D3</accession>
    <name>D5G8D3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00004757001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430042">
        <property type="protein sequence ID" value="CAZ80776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002836539.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8D3"/>
    <dbReference type="STRING" id="656061.D5G8D3"/>
    <dbReference type="GeneID" id="9185996"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004757001"/>
    <dbReference type="eggNOG" id="ENOG502QTS5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002718_1_0_1"/>
    <dbReference type="InParanoid" id="D5G8D3"/>
    <dbReference type="OMA" id="DNTIKER"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055589">
        <property type="entry name" value="DUF7165"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23749">
        <property type="entry name" value="DUF7165"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69322">
        <property type="entry name" value="Tricorn protease domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7165" evidence="2">
        <location>
            <begin position="127"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="425"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="592"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="692"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="837"/>
            <end position="1040"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="448"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="616"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="837"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="885"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="915"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="926"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="947"/>
            <end position="958"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ80776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1040" mass="112419" checksum="DB8B15E49DA67DB6" modified="2010-06-15" version="1">MASPVRKKPLPVRLPLSPQNVDFKGKGKGKGKGKGKEKECGSGFERLPREIIQQILYAADPNTFDSLTLVNSTWHKASRTPLLYAHQLRKSGAVDVPDEPSEDSLPNLIQRFSAKARRELFETILHPQITEIQLICEASSSAAAFPGGEPFRFEFSPRGRHLLAFSSSRIFVLDLSKPEVDVKRELKIGKRPVSAAIVDDGTAIAVLSSNHHVTLYDLTRDKARILKAILLENEPRTIALSPGAEVLGAAYNGGIEVYSLLPDAVASDRRAVNCDTVDSLAFSSDGTILLGTTLNSRSPTTVIVSAQHFSTDLPMEGLAQLWTTQVLFPRSSRDSSHASLLQGSHAEDENTWTFTYDRVYETFRAVRVDDLRNGHTYFTGPSSESLGIVPPTTLPAPTRDGRMVAAGFGGKVWVYGIPGKLDLPPEGSEGGSVNSVSATPERSNSTAGNGGTGSGAGGLTWERGQLGAINVPQWQVLCDKMRNVFIRGREISEVHSLAGLRFVQCANGTERLVAVAGGGVDGLLGEVGEEEFLAIGGGRVMLYDFGRRPGVGETTVMTLEIGDGIQGPVETLEEQQNDIEVEVDLVRRRTVAQQRKGDQRQSALRYARSDTSSVSPPLPPVSRQPQPSPPRPSSASAISRLSSFDENDAGYEGMDAIPYYHGDPRSRGTLQRAASAARNVPQTATSARYLRVVGPDGRPLQNQTMPGFREEEDWVPPPPPYTPKNDNERPLPPHLLQSLQGPPRFNSLADASEEPPRASSALSRTRSTFGAIVRRPSVFRISTNNQPNLSVQPTHSYADISHSRSAPVSPAVGNPGAVHMGIFARTEEPVYMATQQHNQYSRRGSASDILTSPPAAGSVPGMYRESSAPLPRQSHNDPAGHYTPLPTPLPPLPQPPQAYYGNSLLPDRLPPQPRSPTNRRNSAQYRPMPTRRPSPGRSPSSGRRVNLQESYAPNSSLLVPSESGHRRGPSSRSGSVRNTGGGSAGAGSIRVGRGVCGSIRRSSSRPERSAAANVRERKKRRGQRRAVEEQAGDKMGCVIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8L7</accession>
    <name>D5G8L7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00003002001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430047">
        <property type="protein sequence ID" value="CAZ80860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836669.1">
        <property type="nucleotide sequence ID" value="XM_002836623.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8L7"/>
    <dbReference type="GeneID" id="9186228"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003002001"/>
    <dbReference type="eggNOG" id="ENOG502S8FZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070842_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8L7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00118">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.350.10">
        <property type="entry name" value="LysM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018392">
        <property type="entry name" value="LysM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045030">
        <property type="entry name" value="LYSM1-4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036779">
        <property type="entry name" value="LysM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20932">
        <property type="entry name" value="LYSM AND PUTATIVE PEPTIDOGLYCAN-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20932:SF31">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54106">
        <property type="entry name" value="LysM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="263" mass="28953" checksum="4220DF7F34A02A9F" modified="2010-06-15" version="1">MVPVPYSTCLNKTNTMPLPAYASSYQIHCSTCTYSVDRQKSDSRHLSCCGRIVCGDCIAKNPRFGIYCPFCQSPFPKRSGDGGGEAERETPFRPLPPPYAPSAPSPPPPSYSTNGGVIHYVRAADTLPTIALQYNLPLPLLRSHNRIFSDHLLQGRRSVDIPSPPYVGPSLSPAPEDVEGEGRKAATKRFQLKTKCLDADVADVYLQEAGWDEELAVKDWEMDDKWVRDNPMLERSVGKGKGKEVVAGKKDCGSKWGLRGFGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5G941</accession>
    <name>D5G941_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00004972001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430053">
        <property type="protein sequence ID" value="CAZ81034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836843.1">
        <property type="nucleotide sequence ID" value="XM_002836797.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G941"/>
    <dbReference type="GeneID" id="9184321"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004972001"/>
    <dbReference type="HOGENOM" id="CLU_2724043_0_0_1"/>
    <dbReference type="InParanoid" id="D5G941"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ81034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="72" mass="8052" checksum="65F9A0DC3BF14A1A" modified="2010-06-15" version="1">MDNCDANGIPVLIQSPERPANTVISSLSLLSLHGVCTHTHNYTTDKLSKSEVRFFFSFILLMINPIEQSLVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>D5G971</accession>
    <name>D5G971_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00003178001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GST superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430056">
        <property type="protein sequence ID" value="CAZ81064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836873.1">
        <property type="nucleotide sequence ID" value="XM_002836827.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G971"/>
    <dbReference type="STRING" id="656061.D5G971"/>
    <dbReference type="GeneID" id="9184350"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003178001"/>
    <dbReference type="eggNOG" id="ENOG502S039">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039745_0_0_1"/>
    <dbReference type="InParanoid" id="D5G971"/>
    <dbReference type="OMA" id="IPYTQCL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00570">
        <property type="entry name" value="GST_N_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.110">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058268">
        <property type="entry name" value="DUF7962"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051:SF8">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE GSTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25907">
        <property type="entry name" value="DUF7962"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13417">
        <property type="entry name" value="GST_N_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="2">
        <location>
            <begin position="7"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="3">
        <location>
            <begin position="105"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ81064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="332" mass="37382" checksum="B8E1E037304558B7" modified="2010-06-15" version="1">MSTQPVPTIILFHYHYSPYAHKITWYLQLRGIPYKQCIQPPMMPRPDLAELGIKYRRIPLLSIGNEMFCDTSLIVRVLEEKFPKGRLGENGDAWEEWEAWSDQVFPYAAALIPSNLPLMKDEKFVKDREDYSGRSYRKEDVDAGRERAFERVRGFYGHTEKVLSDDRDWILGDKMTLADVEAIWPLEWLTTLPGALDSTVTADTYPKTFSWVKRFTALFPASKSVKIPTITGAEAKDLILHHEQASTSSTSDTGVPDNDPSGVALGEEVEVTPVDTGKNHPQRGRVRALVKDRIIIEVTPTEACAGGGSLKVVFPRKGFEIKSVNTKGNARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9C1</accession>
    <name>D5G9C1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00003311001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430058">
        <property type="protein sequence ID" value="CAZ81114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836923.1">
        <property type="nucleotide sequence ID" value="XM_002836877.1"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G9C1"/>
    <dbReference type="GeneID" id="9182865"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003311001"/>
    <dbReference type="eggNOG" id="ENOG502QPQC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_258207_0_0_1"/>
    <dbReference type="InParanoid" id="D5G9C1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051647">
        <property type="entry name" value="Mediator_comp_sub12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46007">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46007:SF11">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="615"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="679"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="746"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="827"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1141"/>
            <end position="1164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1320"/>
            <end position="1343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="191"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="206"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="296"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="337"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="350"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="397"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="421"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="462"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="489"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="619"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="637"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="683"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="695"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="710"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="827"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1148"/>
            <end position="1164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1343" mass="147407" checksum="61815CAA8072C05A" modified="2010-06-15" version="1">MDGEREREREREWDREPRKNPELPPIDTHAQHRREHYRPQPLLTPLPDGPAPPPAPSCPPPDTPNSEFGSPGAPPYLRSGGYRGIINIGLMGKKLRDDGDSGTPTGFLRTHRGKKGSDESSRGPPPLRRRVNSSSSSSLGKTERPGSAEEDDPIPTEYRPQKRIDEDVGEEKDENEEVSNSVKKGLGLRFQSPSGIPSQQPIGNGMAQQLQQPQQPQMAQNTQNLQDSQKSPEKPGFVRSLPTGGVVTQQRSPAEVGLGTYLYTKPVPPPTGNNPGTTNLRFPGANHGHYVTSPLASGSPGSATSPGSSGGIGGSTRESVMRGHWADVTAAVARPVQQQQQQQQQQPQQEQRKVNMDERSPQHAPPPQQISQMPQRGDFVAGGFDSLQQRTQLGGVPPSHQQQSPQRIQQQQSPPMPPTLIPQHQHQHQQPLEQQQPPNMQQQQQQPGPLPDYGDSGAYKAFTQNLQKPLVTAQPQAQRRVQIRSPPELMDEQNDDEGTNPSESSTPEIPALGARVKFPPRSNSTEDHLFNSFSEILPSLPSTHVHSLLNFAFYLSEDALVADKAEKLRISASNTPSESGWRAVDEEVGVLREQRLRRWKEWVKMEGAERTRPVGLLSKGKEEGRERQRLGLGGEENPFDTETDEDDGEGLGYEGLQPLPPPPRLPEIENLDLKSAGMDAFGGHRHHQRQHQHRHSDQYREMSERKDVPPDAPMPPQQTPSPTPTIDQVANAPKVDLELQMPRPVPSVPAIRIDPSARRRFDDDNSTVASEAHSRGGQEDTVGNAGNTPFPFTFPKVGSGGNYGPQEIRPIDTKGLVAIISSFNRGNSAANGGPSPSGGDPPPSTADIPPFLHMAYAALDTAVNSPSLDTYLSEARRSLETMTSNRLTVFNNESHTRRHRNQAQTAAVYNTGRFTFADIERMRDEFEQEENRKKMEVDKEIYLMFENEYVEVAYKDVKGQLEVLGGKWYEEVKVWLFNASTAASSGGRNSDGGSTAVGTGSGGTALEYHLLLEAIDLLNKLHITMEEHEHVLQSLVSDRNARYLQVSVQPLMTVGETARAADAERRFWIDEQERQLRGKLEGVKRAKEHAKAVERVVSELVGGLRNRFKDVVEAIYAVIFQFPPGVLPQLFRRFFDSNPGPGAIHSQPNSQHPGSESSSPTSSSYTIPKEVIQMMADSIQTLGEIVGLIKLAMDFQNAPQIRVCEATTALQVAEDHARNNGVTAPAASAAACSQGLAIRLPAALKRIREEQVEIGEKGMGEMMEALRKLQNFLNTQSHQGGPPGDEWGYRGQVGPSGGVPSLSSMIGSFRARPGQTNSLLISPGGYSPSGYSPARSSLWNSPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9D3</accession>
    <name>D5G9D3_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Inclusion body clearance protein IML2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Inclusion body clearance protein iml2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00003254001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Inclusion body (IB) resident protein that interacts strongly with lipid droplet (LD) proteins. Involved in LD-mediated IB clearing after protein folding stress, probably by enabling access to the IBs of an LD-stored soluble sterol derivative that acts as a chaperone in inclusion clearing.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with lipid droplet proteins.</text>
    </comment>
    <dbReference type="EMBL" id="FN430059">
        <property type="protein sequence ID" value="CAZ81126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836935.1">
        <property type="nucleotide sequence ID" value="XM_002836889.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9D3"/>
    <dbReference type="FunCoup" id="D5G9D3">
        <property type="interactions" value="123"/>
    </dbReference>
    <dbReference type="GeneID" id="9182877"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003254001"/>
    <dbReference type="eggNOG" id="KOG3783">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014926_1_0_1"/>
    <dbReference type="InParanoid" id="D5G9D3"/>
    <dbReference type="OMA" id="WNGYNRM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019412">
        <property type="entry name" value="IML2/TPR_39"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31859">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 39 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31859:SF1">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 39C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10300">
        <property type="entry name" value="Iml2-TPR_39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="176"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="114"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="145"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="153"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00043897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ81126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="605" mass="68371" checksum="9FB8447874F8FA4E" modified="2010-06-15" version="1">MNFLRKKINKNQDMASDRLADAEGSADRDQKKAIREKIQRSSFPPGTEFALAHAESQLMSAVVGVLSESVVESMKAFYKMRSAYKTLEGLQAHVAVALPNTPLARLDGMGGSKVSLPKSLKAPKRGSDHDGNSLNAGMAGLDMRPASASEVPSRNSSASRRSATSLDSQYNSMDKFIISGANMCFGMLLLILGMVPPSMKRIMAIVGFRGDRERGAEMLWEAAKFDNIHGAIAVLTILQYYGNMAQFSDITPDDNHPSGIGYPADRCHEALGWIRRRYPNAALWKLEEARMEAVKGNLENAVTMLSVPVVTQMKQVEALILFEKSLNQMFLHRYEDVAAGFIKLMTLNNWSHALYNYFAAVCYIELYRQAKTSDTEKAEGWAKKAEDLFERIPSFMGKKRFMAQGLPVEVFADKKIKKWVARSKERGIRLADAVGVSPVEEMMFFWNGYRRMDQENFEISFQALQYWGDHEMENDTIDEQAIRWLLLSAVMRNAGKWEKAREYLEKIQKVDRTQFRGTLVNDYMIPAAYYERAVGIWKEAKEHTPEMLAEMRVLLDKAANYGTFELDARIGMRVTTALDTITRYEVWLKEHKETGIAEKAPKGSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9E8</accession>
    <name>D5G9E8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00003274001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430060">
        <property type="protein sequence ID" value="CAZ81141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836950.1">
        <property type="nucleotide sequence ID" value="XM_002836904.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5G9E8">
        <property type="interactions" value="1078"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G9E8"/>
    <dbReference type="GeneID" id="9182891"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003274001"/>
    <dbReference type="eggNOG" id="KOG1896">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002414_2_1_1"/>
    <dbReference type="InParanoid" id="D5G9E8"/>
    <dbReference type="OMA" id="PMTKFKL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058543">
        <property type="entry name" value="Beta-prop_RSE1/DDB1/CPSF1_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050358">
        <property type="entry name" value="RSE1/DDB1/CFT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004871">
        <property type="entry name" value="RSE1/DDB1/CPSF1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10644">
        <property type="entry name" value="DNA REPAIR/RNA PROCESSING CPSF FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23726">
        <property type="entry name" value="Beta-prop_RSE1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03178">
        <property type="entry name" value="CPSF_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RSE1/DDB1/CPSF1 second beta-propeller" evidence="3">
        <location>
            <begin position="607"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="domain" description="RSE1/DDB1/CPSF1 C-terminal" evidence="2">
        <location>
            <begin position="1065"/>
            <end position="1431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="201"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="507"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="555"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1314"/>
            <end position="1337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="566"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="584"/>
            <end position="596"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ81141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1468" mass="160297" checksum="C6C118EC77A8FDD4" modified="2010-06-15" version="1">MPEVYTELTAPTSVSFSLKLPFTSATSENVLVAKTSLLQIFTTTTYETELNSALADAKQPGDIDRRILDADEEQTFAADIALQRSQVESVTKLVLVAEYPLSGSVTGLQRIKLLSTRSGGEAVLASFKDAKCSLMEWDPETNSITTISLHYYEREEFCSPVVSDGLPTELVADPGSRCAALRFSGDMLAIIPFRQREDEELSLGRGDADEVMGDEDGDNDDWDPEMAGTARGEDTIMGEGDVKTTDATEGKDRPYHPSFVLSVSQLDDAISHVISLTFLHEYREPTFGILYSPRRTWTGLLAAEGRKDTISYIVITLDLEQKASTPILSVSGLPYDIFKVVPLAPPTGGSLLVGGNELIHVDQAGKTTGVAVNPFCRRSTGFAGLADQSDLCLELEGSQVVELESEGGDMLLFTKRGEGVIVGFRMDGRNVSGVKITKLNNHPGSIVGGRVSTAVGLGGRRLFVGCIEGDARVLKWRRKGERKKAGEGIKEEVLENEDEDDVYGALEDMDDDLYGGGGDSSFRKDSLTNGRRNSEAKSQGEYIFQTHDRLTNLGPFRDITLGKPTFPEESRERQKGVSPELELVTTSGPSNTSEDSGISIIRKSISPTIVGRFDFPQCQALWTVRARSANTSNAAVGLGGEEDDRSVEESFDRFLFVTKNDESQVFRVGDTFEEVRGTDFESEGETIEVGVVGNGMRIVQVVSEQVRVYDCDLQLSQIIPMFDEETGEEGPNVHRARVCDPYILLIKVDGSPAVYKMDSTNLELAEERADAIKFDKYQSGCIYASTKGIFIPLDAPVENVKDYLLFLLTVEGGLQIYDLSNPVTPLFSAESFNTLYPLLRTDNPTSPTANREKHRSKQLIIEILVADMGDSIFKEPYLIARSSNNDLTFYKPFISSSPSTLRFIKSPNPHIASNELSLSAGTKNIFRPLTAVYNLAGYSAVFLPGADPSFVIKTAKSSPRIHKLAGTGVRSLSSFHSAGADRGFVYVDSLGIVRVALMPAEFTFDGNWGYKKVTPGEHVQSLAYFPPMNVYVISTSKRQPFDLAEEDGNIAKDDTTLQPEIDSGTLKLLSPQTWTAVDEYKFAHNEIALVVKTISLEVSEHTKERKQLVSVGTAIFRGEDHSARGGIYVFEVIEVVPEPNRPETNRKLKLVTREEVKGTVSAICGVNGYLLAAQGQKIMVRGLKEDQSLLPVAFLDMCLYVSVAKNLDGMILFGDFMKSVWFAGFSEEPYKMTLFGKDTQKLEIISAEFLPDGNQLYFVVVDAESNIHTLQYDPEHPKSLAGQRLIRRADFFSGHEISTLTMLPFSPYSLSASSNSHLPADATDTSPLHHHHQNQQQQQEYFVLAGTQTGSLAMIRTIPETAYRRLNIVQGQIVNGEEHVAGLNPREYRAVVNYSGGGGGGAGGGGWGGSGGGVGGDTMRGVLDGGLVSRWIGLAEGRKGEVSAKAGCGVQGIRGDLKRVVELEVGYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9M7</accession>
    <name>D5G9M7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00003332001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430063">
        <property type="protein sequence ID" value="CAZ81220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837029.1">
        <property type="nucleotide sequence ID" value="XM_002836983.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9M7"/>
    <dbReference type="FunCoup" id="D5G9M7">
        <property type="interactions" value="611"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G9M7"/>
    <dbReference type="GeneID" id="9185356"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003332001"/>
    <dbReference type="eggNOG" id="KOG0118">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_523975_0_0_1"/>
    <dbReference type="InParanoid" id="D5G9M7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000145">
        <property type="entry name" value="Putative RNP domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050374">
        <property type="entry name" value="RRT5_SRSF_SR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003:SF3">
        <property type="entry name" value="FI21236P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003">
        <property type="entry name" value="RNA RECOGNITION MOTIF RRM DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072017" evidence="4">
        <location>
            <begin position="18"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="327"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="87"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="284"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="135"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="147"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="171"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="310"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ81220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="520" mass="57679" checksum="09A2D5945CAEFADF" modified="2010-06-15" version="1">MYFLYFFVFSPFSPLFSLPVVPEDHAITRQGFSRSLPSLFSHPARAFHRKNPEERFRHQQPSSPFQSVQNHPAPWFVSITPFSPNRSKLFPLDSQKNPLHARSPPPPHKAPMESDSYRPAALEGGRSIEREYRHRDRSRSPRRRSPDHRDRARSRSRDDYRRRSSRSPMSGSGGSGYQGSSGGAGGPRRSYEERAAAKDAMMQSLRDSSQQDRRVYVGNLSYDVKWHHLKDFMRQAGEVLFADVLLLPNGMSKQLQDSQTAVNTLSNQNLMGRLVYVREDREAEPRFTGQQGTRPPYPGGPPGSSYPGSGYSGGGGGGGGVPGAGGRQIYVSNLPYTAGWQDLKDLFRQAGAVVRADVHIGPDGRPKGSGVVAFESPEDARNAIAQFNNYDWQGRQLEVREDRFAGSMTIPLCRLTSSPITPLVGVNAVRQFTSAISRGLPAMMTWSSCLIRLERSNVLRFSTSLTEDPGNAETAISKFTGYLYGGRPLGLSYVKYNSFGSNGADAMEVTEPTLTQDQMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9P4</accession>
    <name>D5G9P4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00005009001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430064">
        <property type="protein sequence ID" value="CAZ81237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837046.1">
        <property type="nucleotide sequence ID" value="XM_002837000.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9P4"/>
    <dbReference type="STRING" id="656061.D5G9P4"/>
    <dbReference type="GeneID" id="9185374"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005009001"/>
    <dbReference type="eggNOG" id="KOG4391">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043841_1_0_1"/>
    <dbReference type="InParanoid" id="D5G9P4"/>
    <dbReference type="OMA" id="VIRIHAM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008474">
        <property type="term" value="F:palmitoyl-(protein) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277">
        <property type="entry name" value="ALPHA/BETA HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277:SF64">
        <property type="entry name" value="SUPERFAMILY HYDROLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G01760)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ81237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="308" mass="34889" checksum="052F2229CDEDA793" modified="2010-06-15" version="1">MVIFQNKIIYMPGIPIGAKREKIEAYQESCMGIDWHGRFRDIKTEDGRLLSWVEATVFSRAPAGFAGSRRNVIRIHAMAKINRVYRNASSTPPRLPLISAILSSLKTQNSSTTHTVIIPSYRGYWTSTGSPSQSGIEKDMKAIFRHLESYSTPGTEFILWGQSIGCGIALKGWANYLRAPKCRVPIHVKGLIMETPFISISSMLLALYPQRWLPYRYLSPFLRNHWDVREAVNEIAEKNVRVDIVALRAEKDELVTEAEAVEVEGILERILRKDRHIRRAVITGALHVECVAKAQGRGEIISFLKDTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9R9</accession>
    <name>D5G9R9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00005043001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430064">
        <property type="protein sequence ID" value="CAZ81262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837071.1">
        <property type="nucleotide sequence ID" value="XM_002837025.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9R9"/>
    <dbReference type="STRING" id="656061.D5G9R9"/>
    <dbReference type="GeneID" id="9188505"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005043001"/>
    <dbReference type="eggNOG" id="ENOG502S6P7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013452_1_1_1"/>
    <dbReference type="InParanoid" id="D5G9R9"/>
    <dbReference type="OMA" id="FMNFFDF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14688">
        <property type="entry name" value="bZIP_YAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40618:SF1">
        <property type="entry name" value="B-ZIP TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40618">
        <property type="entry name" value="B-ZIP TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="183"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="330"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="628"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="94"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="340"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="394"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="664"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="690"/>
            <end position="704"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="784" mass="86113" checksum="53FC7D9CA87523AF" modified="2010-06-15" version="1">MDSSLGAQAGLRSFLPTYIPTTREQRDRIMSAGNNIHPNPVHPQYLQPRQQHTSFPGSPHHSGHHSGGTSPYSSTFPMNSMNPSLPAPASYAQSLPSRASSHPAPSSNSQQQQQQQQQQQQQGGYLDPAAYLTMGGVMGMDMDPIPMGSNMDMDLSILSAEEHGDLRVLHERGRARDRMHAERLHSMPHPDGPDGAGRGRGAGGGRRKRSGSDDSVHESVGVARGTNTGNRKQRGRPRLDTKDENAADRRRTQIRLAQRAYRLRKETTISSLRNRVTELETAIDGMQSTFLELHESAMKCLSKSNDAQTLGYGTNLKELTERFLSLAKGALGSSEGADSHDDDEGEDENNGEDHSGVGVKRRRSRDEDGGPGSGRMQTNGPNGVANYGGYEVSYTRQNNSRDSFNNSNGTQSRSNGNGNGRSEATTGHMDSSPTSNPYSDAYVSSSPESGYANYVQPNGDSLQAASQFRQEGPLIYEGFTYSFHETSFSRRLHRAALEHAHYMLTSPDADPQEVARMFAYTFCYLSKADVIRVVNRLLSATTKESLHVRDYPEILPKSIRPEIVEAYLCTPDTSVFTSGLTDDDMKDFDLRLEAKKMMLKMGLDQKFLRPDEVEKYITELGILTSSQQQQSHLIDPSLAETSKPRSSSQQQSKQILEVPEGSTDCLTANRMTTPSYLIHPAAAPSPSPSPSANSRESRSPSSSPHLTLEKKLRLFDTHSPPTARDELDTPTSTNILEGVKKTIDIDVFVNQLVKKGVCLGRFPGFKKCDVEEAIRAAGFRAGVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5GA06</accession>
    <name>D5GA06_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00003481001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the alkaline ceramidase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430068">
        <property type="protein sequence ID" value="CAZ81349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837158.1">
        <property type="nucleotide sequence ID" value="XM_002837112.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GA06"/>
    <dbReference type="FunCoup" id="D5GA06">
        <property type="interactions" value="558"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GA06"/>
    <dbReference type="GeneID" id="9187047"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003481001"/>
    <dbReference type="eggNOG" id="KOG2329">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063293_2_0_1"/>
    <dbReference type="InParanoid" id="D5GA06"/>
    <dbReference type="OMA" id="SIDWCEL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046514">
        <property type="term" value="P:ceramide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008901">
        <property type="entry name" value="ACER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46187">
        <property type="entry name" value="ALKALINE CERAMIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46187:SF3">
        <property type="entry name" value="ALKALINE CERAMIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05875">
        <property type="entry name" value="Ceramidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="66"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="98"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="146"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="185"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="229"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR608901-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR608901-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ81349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="304" mass="35117" checksum="AFE526E9E7AD3690" modified="2010-06-15" version="1">MWWGIPYGLEKVHRWGPETATLNWCEEDYYLTTYCAEIINTLTNGMFIYLGWKGLRNCISEGHDRIFLITFLGYLLVGCGSFLFHATLWYSMQLVDELSMIYTTCLMCWATFGYGHTPTIQTLLGLLLLTIAGSITLIYHHLQDPVFHQNAYALLTTIVLFRSWYLMETRLRSTQSATVTRMWTMVRYGLSFFLSGFLLWNADNAYCSQLRLARREVGMPWGWLLEGHGWWHLLTGWGAYYYIVYGIWLRSCLDGRQGEYECIWERVWSLPVVRRRKGLAVNAKANGVGNGVSTGPNGEVKKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>D5GA97</accession>
    <name>D5GA97_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 16">Elongator complex protein 3</fullName>
            <ecNumber evidence="16">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">GSTUM_00005195001</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Catalytic tRNA acetyltransferase subunit of the elongator complex, which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine). In the elongator complex, acts as a tRNA uridine(34) acetyltransferase by mediating formation of carboxymethyluridine in the wobble base at position 34 in tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>uridine(34) in tRNA + acetyl-CoA + S-adenosyl-L-methionine + H2O = 5-(carboxymethyl)uridine(34) in tRNA + 5'-deoxyadenosine + L-methionine + CoA + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:61020"/>
            <dbReference type="Rhea" id="RHEA-COMP:10407"/>
            <dbReference type="Rhea" id="RHEA-COMP:11727"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74882"/>
            <dbReference type="EC" id="2.3.1.311"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:61021"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16 17">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="16 17">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the ELP3 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="16"/>
    <dbReference type="EMBL" id="FN430075">
        <property type="protein sequence ID" value="CAZ81451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837260.1">
        <property type="nucleotide sequence ID" value="XM_002837214.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GA97"/>
    <dbReference type="FunCoup" id="D5GA97">
        <property type="interactions" value="975"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GA97"/>
    <dbReference type="GeneID" id="9185733"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005195001"/>
    <dbReference type="eggNOG" id="KOG2535">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025983_2_1_1"/>
    <dbReference type="InParanoid" id="D5GA97"/>
    <dbReference type="OMA" id="TFETRPD"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106261">
        <property type="term" value="F:tRNA uridine(34) acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140018">
        <property type="term" value="P:regulation of cytoplasmic translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002926">
        <property type="term" value="P:tRNA wobble base 5-methoxycarbonylmethyl-2-thiouridinylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000003">
        <property type="entry name" value="Elongator complex protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039661">
        <property type="entry name" value="ELP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034687">
        <property type="entry name" value="ELP3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056591">
        <property type="entry name" value="ELP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032432">
        <property type="entry name" value="Radical_SAM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01211">
        <property type="entry name" value="ELP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135:SF0">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23613">
        <property type="entry name" value="ELP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16199">
        <property type="entry name" value="Radical_SAM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005669">
        <property type="entry name" value="Hist_AcTrfase_ELP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01086">
        <property type="entry name" value="elongater_protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00344">
        <property type="entry name" value="ELP3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="14 16" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="11 17" id="KW-0408">Iron</keyword>
    <keyword evidence="12 16 17" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 16 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 16" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 16" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="5 16" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Radical SAM core" evidence="19">
        <location>
            <begin position="99"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="18">
        <location>
            <begin position="413"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR005669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR005669-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CAZ81451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="571" mass="64803" checksum="9B02E04E07FE05CE" modified="2010-06-15" version="1">MPATMVALSTRPSKPNKKGVVLPSERERYIQACADIAHRLIQLHEENGGGKDVNLNALRGQISKKHKLKTIPPLTAIIAAIPEHYKKYIVPKLIAKPIRTSSGIAIVAVMCKPHRCPHIAYTGNICVYCPGGPDSDFEYSTQSYTGYEPTSMRAIRARYDPFEQARGRVDQLRQLGHSVDKVEFIIMGGTFMSLPESYRDGFIASLHNALSGYTTENVDEAVRLAEQSETKCVGITIETRPDYCLDQHLSSMLRYGCTRLEVGVQSLYEDVARDTNRGHTVKAVCETFKLAKDSGYKVVSHMMPDLPNVGMERDLDQFREYFENPDFRTDGLKIYPTLVIRGTGLYELWRTGRYKNYTPNALIDLVARILALVPPWTRIYRVQRDIPMPLVTSGVENGNLRELALSRMKDFGTTCRDVRTREVGIQEVKNKVRPDQVELIRRDYTANGGWETFLSYEDPKQDILIGLLRLRKCSDRTFRPELIGQPTSLVRELHVYGSAVPVHSRNPKKYQHQGYGTLLMEEAERIAREEHGSRKISVISGVGVRKYYAKLGYHLDGPYMSKMLDEHKDEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAE6</accession>
    <name>D5GAE6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00005131001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430075">
        <property type="protein sequence ID" value="CAZ81403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837212.1">
        <property type="nucleotide sequence ID" value="XM_002837166.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAE6"/>
    <dbReference type="GeneID" id="9187101"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005131001"/>
    <dbReference type="HOGENOM" id="CLU_3410810_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAE6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ81403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="29" mass="3267" checksum="D710B99A9E01FA52" modified="2010-06-15" version="1">MHCRGVQSTALKRGTSCDYNWCNYARTSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAG4</accession>
    <name>D5GAG4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00003630001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430076">
        <property type="protein sequence ID" value="CAZ81507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837316.1">
        <property type="nucleotide sequence ID" value="XM_002837270.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAG4"/>
    <dbReference type="GeneID" id="9187447"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003630001"/>
    <dbReference type="HOGENOM" id="CLU_2998156_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAG4"/>
    <dbReference type="OMA" id="MEHRERR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="57" mass="7584" checksum="804D188C818CE73D" modified="2010-06-15" version="1">MIKRKGMEHRERRKKKEKRKKKKEEMRRKKSKRKKEKRGRWKRRKRVRKRNKKKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAH5</accession>
    <name>D5GAH5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">7-dehydrocholesterol reductase</fullName>
            <ecNumber evidence="18">1.3.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Sterol Delta(7)-reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">GSTUM_00003588001</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24 25">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>7-dehydrodesmosterol + NADPH + H(+) = desmosterol + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:46740"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17737"/>
            <dbReference type="ChEBI" id="CHEBI:27910"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:46741"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>cholesterol + NADP(+) = 7-dehydrocholesterol + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23984"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:17759"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.3.1.21"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="21">
            <dbReference type="Rhea" id="RHEA:23986"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Steroid biosynthesis; cholesterol biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 23">Belongs to the ERG4/ERG24 family.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.21" evidence="18"/>
    <dbReference type="EMBL" id="FN430077">
        <property type="protein sequence ID" value="CAZ81518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837327.1">
        <property type="nucleotide sequence ID" value="XM_002837281.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAH5"/>
    <dbReference type="STRING" id="656061.D5GAH5"/>
    <dbReference type="GeneID" id="9187458"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003588001"/>
    <dbReference type="eggNOG" id="KOG1435">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015631_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAH5"/>
    <dbReference type="OMA" id="WGKPAEC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047598">
        <property type="term" value="F:7-dehydrocholesterol reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016132">
        <property type="term" value="P:brassinosteroid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006695">
        <property type="term" value="P:cholesterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="7-dehydrocholesterol reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001171">
        <property type="entry name" value="ERG24_DHCR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018083">
        <property type="entry name" value="Sterol_reductase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21257:SF38">
        <property type="entry name" value="7-DEHYDROCHOLESTEROL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21257">
        <property type="entry name" value="DELTA(14)-STEROL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01222">
        <property type="entry name" value="ERG4_ERG24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01017">
        <property type="entry name" value="STEROL_REDUCT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0152">Cholesterol biosynthesis</keyword>
    <keyword evidence="5" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 23" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="14 23" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="15 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0521">NADP</keyword>
    <keyword evidence="12 23" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 23" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="17 23" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="13 23" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="16 23" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="6 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 23" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="62"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="191"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="274"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="314"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="344"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="425"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00038851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00039984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00042688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="RuleBase" id="RU369120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="CAZ81518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="487" mass="55082" checksum="0BDE1A242CFCC4BD" modified="2010-06-15" version="1">MSTTITEALTGKVPFDVPYTLLSGRHWTTAQAHKPPSNKFVQPPPTAKHVNPAWGRFSGNEWVTVMGCSAIVLFCPLLALLACVCLKHFDGGLLPTLWAFMTTHPVEFIVRYAPRPTAGASAAYAGWLLFQAALYAWLPSPIGYGQRTPAGHLLPYRVNGIHAWVVTHVVSVVAVYTGMLDPAVIAKNWEGLLVVANVYGYLLAVVAYLKAHLFPTHEADRKFSGSMVYDFYMGIELNPRFGEWWDFKLFHNGRPGIVAWTLINLSFAAWQFQLHGYISNSMIIVNILHAFYVVDFFINEDWYLRTIDICHDHYGFYLAWGSMVWLPGTYTLQAQYLARYPVDLSTPVAAAILLIGISGYVVFRSVNYQKDIVRRTNGECNIWGKKAEVIRTTYKTEDGKMHESLLLCSGWWGIVRHANYLGDLVLSYSGCAVCGVHNILPWWYAVFMTALLLQRCVRDHSRCLGKYGEDWERYCGRVKWKLIPGVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAJ1</accession>
    <name>D5GAJ1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00003610001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430078">
        <property type="protein sequence ID" value="CAZ81534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837343.1">
        <property type="nucleotide sequence ID" value="XM_002837297.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAJ1"/>
    <dbReference type="STRING" id="656061.D5GAJ1"/>
    <dbReference type="GeneID" id="9187474"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003610001"/>
    <dbReference type="eggNOG" id="KOG3294">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066296_1_0_1"/>
    <dbReference type="InParanoid" id="D5GAJ1"/>
    <dbReference type="OMA" id="SNRLEDH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13214">
        <property type="entry name" value="PH-GRAM_WBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044852">
        <property type="entry name" value="WBP2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31606">
        <property type="entry name" value="WW DOMAIN BINDING PROTEIN 2, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31606:SF1">
        <property type="entry name" value="WW DOMAIN BINDING PROTEIN 2, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="174"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="222"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="245" mass="26100" checksum="C2C46D88B0F18725" modified="2010-06-15" version="1">MSINWVMLSPTPPHTFIALPNERSLYQSPARTGISLTTTSPHRGTRPLSINSNTGTAYITNQRIIYIPTAKTSDFESFACPIANLHDTHVAPPFFGPNVWKAVVQPVANGGLGGGDRGGVELKLIFKDGGAFDFSTIFERLKEKVLNARETGVSAEAVHLDELPTYEDARTGQPSSSVTTAGAPPSPAATAPAMVSEPPEITVIQADADPSIPSGPPPGYEEAQRESVAEELERQIGQIADARIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAW9</accession>
    <name>D5GAW9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00005340001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430086">
        <property type="protein sequence ID" value="CAZ81662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837471.1">
        <property type="nucleotide sequence ID" value="XM_002837425.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAW9"/>
    <dbReference type="GeneID" id="9187830"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005340001"/>
    <dbReference type="HOGENOM" id="CLU_2833017_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAW9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="66" mass="7378" checksum="EBB89A5AA1FECC0F" modified="2010-06-15" version="1">MPSPSFRSCSAFRLTSSLAFLELAPRFLRGVFFGFAVLLFPTDLGVLTSTLSIVWVWVWVSEGTVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>D5GB30</accession>
    <name>D5GB30_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 12">Mannose-6-phosphate isomerase</fullName>
            <ecNumber evidence="5 12">5.3.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">GSTUM_00005420001</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>D-mannose 6-phosphate = D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:12356"/>
            <dbReference type="ChEBI" id="CHEBI:58735"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="EC" id="5.3.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11 12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11 12">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 14">Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the mannose-6-phosphate isomerase type 1 family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.8" evidence="5 12"/>
    <dbReference type="EMBL" id="FN430086">
        <property type="protein sequence ID" value="CAZ81723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837532.1">
        <property type="nucleotide sequence ID" value="XM_002837486.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GB30"/>
    <dbReference type="FunCoup" id="D5GB30">
        <property type="interactions" value="810"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GB30"/>
    <dbReference type="GeneID" id="9187964"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005420001"/>
    <dbReference type="eggNOG" id="KOG2757">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026967_0_0_1"/>
    <dbReference type="InParanoid" id="D5GB30"/>
    <dbReference type="OMA" id="DIGLFCG"/>
    <dbReference type="UniPathway" id="UPA00126">
        <property type="reaction ID" value="UER00423"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004476">
        <property type="term" value="F:mannose-6-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009298">
        <property type="term" value="P:GDP-mannose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd07011">
        <property type="entry name" value="cupin_PMI_type_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.441.10">
        <property type="entry name" value="Phosphomannose Isomerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001250">
        <property type="entry name" value="Man6P_Isoase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016305">
        <property type="entry name" value="Mannose-6-P_Isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018050">
        <property type="entry name" value="Pmannose_isomerase-type1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046456">
        <property type="entry name" value="PMI_typeI_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046457">
        <property type="entry name" value="PMI_typeI_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046458">
        <property type="entry name" value="PMI_typeI_hel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00218">
        <property type="entry name" value="manA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10309">
        <property type="entry name" value="MANNOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10309:SF0">
        <property type="entry name" value="MANNOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01238">
        <property type="entry name" value="PMI_typeI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20511">
        <property type="entry name" value="PMI_typeI_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20512">
        <property type="entry name" value="PMI_typeI_hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001480">
        <property type="entry name" value="Mannose-6-phosphate_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00714">
        <property type="entry name" value="MAN6PISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00965">
        <property type="entry name" value="PMI_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00966">
        <property type="entry name" value="PMI_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Phosphomannose isomerase type I catalytic" evidence="16">
        <location>
            <begin position="6"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphomannose isomerase type I helical insertion" evidence="17">
        <location>
            <begin position="207"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphomannose isomerase type I C-terminal" evidence="15">
        <location>
            <begin position="322"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="275"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001480-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001480-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF20511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF20512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAZ81723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="407" mass="44124" checksum="F3BE53103E52C497" modified="2010-06-15" version="1">MLPVPLIQLQCGANSYDWGKIGNDSAAARFAAATTQSGFKIEESKPYAELWMGTHPSNPSKDVDTGRTLLELVNSNTALLSHDIAEKYGGKLPFLFKILSIQKALSIQAHPDKKLAERLHSEDPKNYPDDNHKPEMAIAVTPFDGFCGFRPLSEIVSFLESTPPLRALVGESKSDIFIAAVGDKADASDEATIAANKKALKDLFEAVMTSEEEFANLIIRLDGQFPGDIGLFCSFLLNYVKLQPGEAMFLQANDPHAYLSGDIVECMAASDNVVRAGFTPKYKDVKNLVSMLTYSYAPISEQKMKPAPYPRGSNSKSGVPTSILYDPPIDEFAVVKTGLKSGNSEKFQPIEGPSIIIATSGRGKISVGPKTETLDSGHVYFVGATATLELDAEEDLVAFRAFCELAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBK5</accession>
    <name>D5GBK5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="13">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00005677001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAPRE family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ82011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837820.1">
        <property type="nucleotide sequence ID" value="XM_002837774.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBK5"/>
    <dbReference type="FunCoup" id="D5GBK5">
        <property type="interactions" value="659"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GBK5"/>
    <dbReference type="GeneID" id="9185446"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005677001"/>
    <dbReference type="eggNOG" id="KOG3000">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041744_2_1_1"/>
    <dbReference type="InParanoid" id="D5GBK5"/>
    <dbReference type="OMA" id="WIKRFWD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051285">
        <property type="term" value="C:cell cortex of cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055028">
        <property type="term" value="C:cortical microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035371">
        <property type="term" value="C:microtubule plus-end"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061673">
        <property type="term" value="C:mitotic spindle astral microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990023">
        <property type="term" value="C:mitotic spindle midzone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005880">
        <property type="term" value="C:nuclear microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008093">
        <property type="term" value="F:cytoskeletal anchor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099609">
        <property type="term" value="F:microtubule lateral binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051010">
        <property type="term" value="F:microtubule plus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051315">
        <property type="term" value="P:attachment of mitotic spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030989">
        <property type="term" value="P:dynein-driven meiotic oscillatory nuclear movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000742">
        <property type="term" value="P:karyogamy involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000743">
        <property type="term" value="P:nuclear migration involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035372">
        <property type="term" value="P:protein localization to microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1430:FF:000005">
        <property type="entry name" value="Eb1, isoform E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000028">
        <property type="entry name" value="RP/EB family microtubule-associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004953">
        <property type="entry name" value="EB1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036133">
        <property type="entry name" value="EB1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027328">
        <property type="entry name" value="MAPRE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10623">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN RP/EB FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03271">
        <property type="entry name" value="EB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140612">
        <property type="entry name" value="EB1 dimerisation domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51230">
        <property type="entry name" value="EB1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5 9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="11">
        <location>
            <begin position="2"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="EB1 C-terminal" evidence="12">
        <location>
            <begin position="154"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="117"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="130"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ82011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="242" mass="27036" checksum="91F2B3B77E6E38BE" modified="2010-06-15" version="1">MGESRQELLNWLNGLLQLNITRIEQCGTGAALCQVFDSIFGDVPMQKVKFNVNTEYAYLQNFKILTSVFSSKGIDRPIPVERLVKCKFQDNLEFLQWTKKYWDQYYPGGDYDALSRRKGAGSVGGNHPPAARANTSSAAAKRGTTPTVGGARTRTPLGGASTAALQQENSMLKENVTGLERERDFYFSKLRDIELLIQQAVEQDPKIEEDEDGLVKHIQAILYSTEDGFEIPAEEGDDLETF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBT0</accession>
    <name>D5GBT0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00005566001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ81930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837739.1">
        <property type="nucleotide sequence ID" value="XM_002837693.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBT0"/>
    <dbReference type="GeneID" id="9182967"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005566001"/>
    <dbReference type="HOGENOM" id="CLU_2924395_0_0_1"/>
    <dbReference type="InParanoid" id="D5GBT0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072644" evidence="2">
        <location>
            <begin position="24"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ81930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="61" mass="6829" checksum="9E3226EA7E8EE60A" modified="2010-06-15" version="1">MGILGYMLSFFSIFLFLFRVGRGIDGLTEGLRKNCSRGFPSVPTLTREPRNSTETLRITSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBT8</accession>
    <name>D5GBT8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00005574001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ81938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837747.1">
        <property type="nucleotide sequence ID" value="XM_002837701.1"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GBT8"/>
    <dbReference type="GeneID" id="9182975"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005574001"/>
    <dbReference type="eggNOG" id="KOG0298">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000796_0_0_1"/>
    <dbReference type="InParanoid" id="D5GBT8"/>
    <dbReference type="OMA" id="SMIPYIT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001525">
        <property type="entry name" value="C5_MeTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050628">
        <property type="entry name" value="SNF2_RAD54_helicase_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626:SF26">
        <property type="entry name" value="FAMILY HELICASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G09120)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00145">
        <property type="entry name" value="DNA_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="1976"/>
            <end position="2127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="40"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1444"/>
            <end position="1479"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="1646"/>
            <end position="1673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="79"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1461"/>
            <end position="1473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ81938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="2127" mass="237315" checksum="837EA1B227220220" modified="2010-06-15" version="1">MDNYFGKAANRAPIIVPEFVKPRGKGKAVAVEKSLALAPKAKVKKERAPKTPVPKKSAKAHSRSAKGTDDESDFAVSGDAEDAESEATDDVFPTEDEGDSGAAIDDEIDDIVVDKPKSKSKTRVTATGKVKHVSVSKPSGLKIIPREGDLPPISDLQQMFDDIVSRLPDLKSLFGKSGGRKLRVATMCSGTESPLLALGLMGRSMRSQGIGKLDIEHVFSCEIELYKQAYIERNFKPPILFRDVRELGEDVATTAYGAKVPVPGNVDLLIAGTSCVDFSNLNNKKKGLEDVGESSDTFHGMMRWAIRHRPTVIIQENVQGAAWTQMRAEYIEAGYSAEFVSNFDSKNYYVPQTRQRGYLFAVDKAGSGIPNRWIDAMKSLRRPATCTLEAFLLPSDDPRIHQVRMDLATKRDGSKKTARSVEWEKCEGRHQKERLQQKLGLKRPMTQWEEGGVCKMLDFAWQDWGAMQVDRVLDLMDINMLTLAKEGIDCNYKEQFWNLSQNVDRNTFGKANGICPCLTPHMIPYLTDRGGPMIGLEALAMQGLPINELLLTRESTQELQNLAGNAMTSTVVGTATLVALLLSKNVLRPPNDDGMDVDVDDLDISTRIGGDEQLKQRPLDLSTTQAFKISDLLKLALRSSRLCVCEGRSDMTSSLLSRCEECGFSSCERCGGRPEHVYKALSVHDRLAPLAFEDMLKGVLPMRLVVAGITEEFLESLKTANPTAAGHISTKDWSIFISLILDATSGEFRFTNLKRQQIWSVSYDAPAAKLELLLDPSQPEWRLFAKCPTSEGVNSRKRAMCKAPMARMRLSNAQDIMDCGVEVCLPTRYDFDLTLEGEGDLVESWQAQLGLQDSKFAEKKVWSYIRMTVPDEAKECLDRDLSGLWKLLPACGTSNGALHKQVDGDGGAQLFFFLDPTKCGEPKHDPFVFSVSPRRYAYGEQRPLVAKLDPKFRTFGDNTPRVVRCHVDGQWVVAKGSTLKPPSASTQTVGRAIYATPGKKGLEISVTKASCAAANAILTCRVPLENQAEGVWPKGVWKQVDKIHERNTFESLAWLTERVRSMRHLSNWNALDLPEDHTSCERCAPKPPNLRWVMKGNKFVPMEDAKQAAPYERALKNRPSPFVTQLMLEDDSTGILRIGLNIPTLVHRALSRLPSEGREEMPMLSWRLTTDYIPETQLTLPAFELTSNKKDQQHTQPPRFITKLRPEQLRSLSWMLTQEEEDAIPFVEEEISEALLPHLNWRAEGKAERPIQVRGGVLADQVGYGKTAITLGLIDCSRDDVKLPEDVDGRIPVKATLIIVPAHLTNQWPAEINKFTGSNTYKVIKIQSQAHLNALTIAQVMDADIIVVASTLFRSDKYLGNLAGFAATRPVPSSEGRRFNAWQKVALRDLKEQVNNLRGGGAEFVRQRIDDNAKGVNDEDSSAEEMVRNKRLIGKAYVEAVEKQAQAGGKRKRPRVEVSDGEFSSSASGSGPSKRSKMAPVVVGDPWGLRTNAVARDWKRMHAPPFEMFHFNRLVVDEYTYLKGQIHAGITCLKSTFRWVLSGTPPLDDFADVKTISVFLGIHLGIDDDMVGKIDNRKKIKKDRTSVEAFTAFRELRSPGWHTRRHEIAQNFLNQFVRQNIAEIDEIPFEEHIVPITLPAAERAIYLELEHHLQALEMNIRKSKIKVDNDRDKRLQESLGDSASAEEALLKRCSHFVLDFTKEDEENAVQACEVIVKDRKRQLTSCREEIRQRLVDCLKRHREIPKDSFPIASGDHFGTFLKTTLENGIGDPEAGGDIVALINQAKGNGRGPKPFSGIPSVVGVDFLKPVKSSKKTKGKGKADDDDDDVEVYIEPWPKAMEDKVQQLRDRTAVLRALVRKELVGRHRSLRYFRIVRDLQQFGLAEFAKKNSVSNVSCPGCKRKNLPVSEIAVLSSCGHQGCYTCLEAAAHKQECLTPGCDSAARVLNVVKGESLGTEDAKEGIGRHYGMKLEKMMHLIQKLIPVDEKILVFVQFTDLMEKVAQVLKEYKIGFLQIKGSAAAQSKALQQFQESAEKDNIKVLLLNVMDESASGANLTNANHAIFLSPLLTTTDYEYRSCETQAIGRVRRYGQTKKVHVWRFLTENSIDVEIFQDRGGAKFADVEKFVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBU5</accession>
    <name>D5GBU5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00005590001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ81945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837754.1">
        <property type="nucleotide sequence ID" value="XM_002837708.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBU5"/>
    <dbReference type="GeneID" id="9182982"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005590001"/>
    <dbReference type="HOGENOM" id="CLU_998162_0_0_1"/>
    <dbReference type="InParanoid" id="D5GBU5"/>
    <dbReference type="OMA" id="EENDAPW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046529">
        <property type="entry name" value="DUF6594"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34502:SF5">
        <property type="entry name" value="DUF6594 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34502">
        <property type="entry name" value="DUF6594 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20237">
        <property type="entry name" value="DUF6594"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="197"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="224"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="252"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="DUF6594" evidence="2">
        <location>
            <begin position="38"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ81945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="279" mass="30412" checksum="A6A45DF16A6C96B1" modified="2010-06-15" version="1">MGGGGVGDVNRRLSASSGAVSPRAAAPGYSLDEFKGFREIADYMSHDGDCVLVRRFGVLRFRDLLCKQAQVAELERHLSRVDNGELFGQGDVEAVKGLMRELDTKLKVYDEALERCHRVYTMPRPSARSLNKTSSWLRTRGFDLHQQPEEDIVTLDQALITDGPITRIAALIARVVYKKEVTRRAGVDQLSTDRSILVSRALISVIAPVILLVPVVLLCYVRKLWAQIVVVALATVISSFAMMVAKAGNAEVFMATSAYAAVMVVFLGTVADVSGKGKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5GC61</accession>
    <name>D5GC61_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00000520001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430102">
        <property type="protein sequence ID" value="CAZ82104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837913.1">
        <property type="nucleotide sequence ID" value="XM_002837867.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GC61"/>
    <dbReference type="GeneID" id="9184773"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000520001"/>
    <dbReference type="HOGENOM" id="CLU_874904_0_0_1"/>
    <dbReference type="InParanoid" id="D5GC61"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ82104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="318" mass="36172" checksum="E131427E873C41C9" modified="2010-06-15" version="1">MPEDCGRLVSVLAFYVFSCSCFRDSGKHCKPSRDAQSNWKEEIENGPSLFRRRDCSFQSSSPRGQSPAFGIYGGRSNAVYSVPSTADSEDTEHLENLFTEMQEKKTKTERITTVTVTRLPRIHPPVNEYHPPVVSALPGTRTDTAWMRLPPPTARAMRAINRETHSRKDSVRDKYGSAYDATSNIHRLESQSIVRNKLDEWKIVGTLKQAGGTDLRKPEDTVGMSEIPEEEKQHQVPEILSEGHWLKRRRVFTNLPELNGRRLVDIDGAIKFDQNLESPYHSTFSEPTEAIRRPAQVFARPYPIRSMSIGLDDDFYSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDC1</accession>
    <name>D5GDC1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00006144001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430142">
        <property type="protein sequence ID" value="CAZ82514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838323.1">
        <property type="nucleotide sequence ID" value="XM_002838277.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDC1"/>
    <dbReference type="GeneID" id="9183978"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006144001"/>
    <dbReference type="eggNOG" id="ENOG502RZBG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032395_0_0_1"/>
    <dbReference type="InParanoid" id="D5GDC1"/>
    <dbReference type="OMA" id="RPREYHS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="282"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="317"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="358" mass="39807" checksum="49AC6531BE359544" modified="2010-06-15" version="1">MGGTPRKRNELPKIFVKKEPASPDPPSRHRPPRLHLSNGPTPPSGPNAAATPRTANGHLATMQDVGMACLSPGFATHDPAMRDQLQRSISVREQQRSIIESRLQRHVKGGERTGGAEPNSRTDRDEPPSARGGDRSSAHTPRRRPPSSLTIVPPPHRAFANERVVQSAPLHQSFTGRNHPPPSSHHGPPPTTHHHQQQVNRLPPISDVFGLDGPESSRSVRHPDDGTSRQYFPATRPSYPSPNSMAYSRPREHRSAEEAMANISGGREDLLPRIIHYGGHQPPTPPSPPQPQHTPSKLGVHGMHEQQPHSAKRRRTRAEYERDESMGSDHGDREQESKRRKKEEFLSLCARAWELFHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDL4</accession>
    <name>D5GDL4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00001012001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430148">
        <property type="protein sequence ID" value="CAZ82607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838416.1">
        <property type="nucleotide sequence ID" value="XM_002838370.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDL4"/>
    <dbReference type="STRING" id="656061.D5GDL4"/>
    <dbReference type="GeneID" id="9181612"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001012001"/>
    <dbReference type="eggNOG" id="ENOG502S1ZU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_307593_0_0_1"/>
    <dbReference type="InParanoid" id="D5GDL4"/>
    <dbReference type="OMA" id="QTHANCE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="214"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="322"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="421"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="543"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="935"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="85"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="146"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="221"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="333"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="351"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="374"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="428"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="449"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="565"/>
            <end position="594"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="961" mass="102093" checksum="E74B6D1866C8B5A9" modified="2010-06-15" version="1">MSQRRTEDRPSSSHHQNNSNGGVNDTGTFSAPINGTGNRQGRDSARRDRHITVKQNQQNLNITGTGRTRTGSLHHPLPQRDVRKPASSTPSAPSSQKHPKTTTLPPSPAVTRTHRRHTSVIVNPPPPPASFIPSQPKLASPVLLVSRPATSHSSPPSATSSGKRAPARFSSNGIATTSGPPPSLAYRRNIAMDNHFSPHNVGLGVGGGNVHRGGVALPVNGTTGDRSSTSSSTISHLSRRGSLKGRVQGEQSTSRTGSLEPDQTGSNGDDDEDDTGRDSGQTIRGLEDLQSGASEFAGDGGLDGEPGTEDLFLNLARLNSAAGQNENSNGPRPRARLAHHAHHRSSLPADTFSSSPLRASQTRSPGNRGSPVGPRDEIWHDPRAPTPATRYRDRRMSGTTDNLVDQRIVTPRASFVRPLSNVLRRDQSPASSTHTRSFSMAEPGMRSRNGFTNGIAPQSLHSSPNATDQSPGSVIDHDQTDSSGAASRMEHSGGASSGIGGQEDSSSVSTTAPSTVWDELDDLKSRIRRLELTGRIPASGVGAAGNTYSSSGERPRTATTTVTTISSSPRHQNNPSGIGISPPNSTISHPSPNSHPLLRAALTKSKPLIPPDVFRYLEAAAADAVALANVVGCTAPVGSGPPGAVVDRQMRRKADSVCRSLTELCIALTENRSSLGVTNYSHHVAQHVHGARPISRDTALGRECILGRESALGRESILGRESVLGRESVLGGRESILGRDSSVGSHRGLGRLSERKGSIASISTAAGYNSSPRLSLFVDQETPSTPGLLRPRNSLLLKTRQMLDDDEGHEGFRSPSRAATDIMGHRHRNYPISRHMSLTPERSPGPEMSHRRPHYVTNGAALPSPILHGNRRYFSATVDRSDVADRPNGSFEATNSTNERLVATAAADSDYGAARYRSGSLGRAGNRRLTRGLRESVDLDSVQQGGSLGRTPLPGERLLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDZ3</accession>
    <name>D5GDZ3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00001111001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430155">
        <property type="protein sequence ID" value="CAZ82736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838545.1">
        <property type="nucleotide sequence ID" value="XM_002838499.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDZ3"/>
    <dbReference type="GeneID" id="9184531"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001111001"/>
    <dbReference type="HOGENOM" id="CLU_3410811_0_0_1"/>
    <dbReference type="InParanoid" id="D5GDZ3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="29" mass="3499" checksum="35BC015EAC83B905" modified="2010-06-15" version="1">MVYYIYLQCAVFYIALCLSLSNLYRLKSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>D5GE51</accession>
    <name>D5GE51_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00006381001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430164">
        <property type="protein sequence ID" value="CAZ82794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838603.1">
        <property type="nucleotide sequence ID" value="XM_002838557.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GE51"/>
    <dbReference type="STRING" id="656061.D5GE51"/>
    <dbReference type="GeneID" id="9183126"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006381001"/>
    <dbReference type="eggNOG" id="ENOG502S1FV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_408367_0_0_1"/>
    <dbReference type="InParanoid" id="D5GE51"/>
    <dbReference type="OMA" id="WETVETE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="476"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="539"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="673" mass="72101" checksum="8DC3209EBD0105ED" modified="2010-06-15" version="1">MYSLLRISSSTVLVATHEDISLYDTKFSSLQARAPLHQNPTSSTIASRDPPRAISPVSGLDGKPKGQIFLTSYIQDLDLAVGYSQAGIIGVQLTRTGGKSSAKAGTRRSGLLIDSLCRGVEIVDVGISKGQAPVAPGPRKLVSRLASESKAVRKVIRDLKEAKKAGDLQGFEQIFADYVGVSWETVETELGEGTPMVNGTSDLEVVNELSSMGKPVPGLSLQRDGSHLPSIYKPLRYEFVVGVLDMIFEASSDHNSAQCLSVSFCPPNVFRYLIESGNFSETLIRSYSGLVQCLAEFDPSLRTLEWILGVAGEIGLGEVIKAIKIGLDHGNVNICGEDQIVLDVIRSEVMRLALVRLNGFPAEAITKALRTGLGGQDLMGLIHLLRKELLFGRKLHGEVGIGIGIEDIGIIADLLTVALDTVGIGGLVLGNSSDEFIQELRSEVTAVVEGAQEAAALKSILEEVLRHADWRRVTLEKKQADKKQAKKRKQNPKGVKESARGGGSRGEGGQKKGKGVEVNGLQNGEDKMESALGYPAVPSENTPSKNTVGESADKPAEAFVFSKLPPEEAGPQNEIGGIVIKRTREIPAKSVSVAKHLVNSGPMINTDTSVVSPLLPLGVPPAPISRISLVMAQPAENGINLKELRQLNRRKIKEASYRESMVAGVYSVESMLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>D5GE93</accession>
    <name>D5GE93_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00001230001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430168">
        <property type="protein sequence ID" value="CAZ82836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838645.1">
        <property type="nucleotide sequence ID" value="XM_002838599.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GE93"/>
    <dbReference type="STRING" id="656061.D5GE93"/>
    <dbReference type="GeneID" id="9185491"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001230001"/>
    <dbReference type="eggNOG" id="ENOG502SUBI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085820_0_0_1"/>
    <dbReference type="InParanoid" id="D5GE93"/>
    <dbReference type="OMA" id="APSHHYH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42095">
        <property type="entry name" value="YALI0C12166P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42095:SF1">
        <property type="entry name" value="YALI0C12166P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072303" evidence="2">
        <location>
            <begin position="23"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="22"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ82836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="140" mass="14985" checksum="44E948E2ED7D5D8A" modified="2010-06-15" version="1">MPTNRPFLANLLVAFRAHSATAKGSSNNPSSSSPSSKPSAGPKTQSAPASSSTTAPSSTAAMATAAVHTNSSQQQQPTNFTSSRQRRSSSSSTEGFVEPPSGEKWWIGGRNSDGQERFYRLQPVRRQRSCDRLSLDRLSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5GER8</accession>
    <name>D5GER8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00001346001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430185">
        <property type="protein sequence ID" value="CAZ83011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838820.1">
        <property type="nucleotide sequence ID" value="XM_002838774.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GER8"/>
    <dbReference type="FunCoup" id="D5GER8">
        <property type="interactions" value="140"/>
    </dbReference>
    <dbReference type="GeneID" id="9184923"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001346001"/>
    <dbReference type="eggNOG" id="KOG2533">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_0_1_1"/>
    <dbReference type="InParanoid" id="D5GER8"/>
    <dbReference type="OMA" id="LPYVTMW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000034">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000068">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF57">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="84"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="113"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="143"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="206"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="275"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="315"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="339"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="364"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="398"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="431"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="47"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ83011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="495" mass="54856" checksum="2B80D4E997994646" modified="2010-06-15" version="1">MSEGSTIEKEAKHIDGTVTPDSLESSDLACFDEKRTKKLLRKLDWHLVPFLALLYLLSFLDRTNIGNARLAGLEKDLGMSGLDYNVALAVFFPWYVAAEVPSNLMMKRTRPSLWLTIIMVAWGITMTLMGLVTNYVGLLICRMALGLTEGGLFPGVTFYITMWYRRHECGLRMAIFFSAATLAGAFGGLLARGISEMAGVGGKPGWAWIFILEGLVTVIIACIAIWAINDYPETAKFLTPEERKEVHRRLKADRTSLADEYHIKYFFHAIKDWKIYVHMLITIGIYTPLYSFSLFLPTIVKNMGYTNKESQLMSVPPYVFACICTIGAGYLADRSKQRGVYMIAACLTSIIGFTMLISTHKPGVQYVGTFLAAGGIYPNVPMGVAWNGNNIGGSTKRGVGIAMHVGFGNLGGIIAAFAYRSTDAPRYFSGHGLLIATTTMSLSLCCFMTIYLRKENARRDALMASQGLTLDGYSDDMKDSEREKGDNATFFRYTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5GET5</accession>
    <name>D5GET5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00001365001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
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    </dbReference>
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        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ83028.1"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
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            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
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            <taxon>Tuber</taxon>
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    </organism>
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        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
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                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
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                <person name="Artiguenave F."/>
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                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
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                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
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            <dbReference type="PubMed" id="20348908"/>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
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        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
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    <accession>D5GFQ5</accession>
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        <lineage>
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            <taxon>Pezizomycetes</taxon>
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            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
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                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
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                <person name="Stocchi V."/>
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                <person name="Viscomi A.R."/>
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                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
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                <person name="Bonfante P."/>
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                <person name="Wincker P."/>
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            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="13 14">Mel28</strain>
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        </subcellularLocation>
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    <dbReference type="KEGG" id="tml:GSTUM_00007008001"/>
    <dbReference type="eggNOG" id="KOG2068">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_262065_0_0_1"/>
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    <dbReference type="OMA" id="CPNKNCM"/>
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    <dbReference type="GO" id="GO:0030014">
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:ubiquitin-protein transferase activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0016567">
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        <property type="entry name" value="CCR4-NOT transcription complex subunit 4"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="NOT4_mRING-HC-C4C4"/>
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    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12603">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12603:SF0">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14570">
        <property type="entry name" value="zf-RING_4"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    <keyword evidence="2 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 8" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 8" id="KW-0863">Zinc-finger</keyword>
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        <location>
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            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="12">
        <location>
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            <end position="200"/>
        </location>
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        <location>
            <begin position="173"/>
            <end position="200"/>
        </location>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
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    <feature type="region of interest" description="Disordered" evidence="10">
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    </feature>
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        </location>
    </feature>
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    <feature type="compositionally biased region" description="Polar residues" evidence="10">
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
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    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
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        </location>
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    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="965"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="975"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="1022"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1049"/>
            <end position="1060"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="1085"/>
            <end position="1108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ83348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1293" mass="139007" checksum="E76AE0F51922F4E1" modified="2010-06-15" version="1">MSRVQQDTFIDDTEEFCPLCVEEFDIQDRNFRPCPCGYQICQFCYNNIKNNLNNLCPACRRPYDEKTIEWKGISAEEMRADQNKKNRQQNEKRKLEAQKREIDALNRKHLSGLRVIQKNLVYVTGLNPRIPEEDLLQTLRGKSYFGQYGKIIKIVIRQWVSSQNGDRTLRATYGTTKYCSAYLRNEACPNKNCMFLHEPGEEADSYDRQQMSTFTVRQQEGAPSQARPPQHQPPITTATPTHPVHPPPTQQQSAQMARVSSHQGGSSSIGDNGESSALPSTANWAKNPPTPAARSVAPVIQPQGSAILSKGSTPSPRSVPAAPMVQTQSQESGKSGSSLGKAPASTGSGGNSGPGPVGKPPGGLATNNQVPTQTSEASSSSVQDSTTNALRSPTKPSPPELPIPVQEHLKRLENCVRAVSNPKFRFVFSTEGMTEEDIADLEKMPPMFDRHGGRRRRENAERLRAQQQQQHEEAESDIPQPQPPVGTMAAAPGLVPLNLTGRGNTPSQRDLLKEVAASHPQQQPLGAFQQQENLQHQQAMRVLTPGGILHPQQQQQQQQQIQQSFSPFGAMGNPQQQQQQTHGGSLHTRQTSRYNFANDNPAVASTSVNPRGNIAHMTEQLRMMPQQAQQQQQQQLHNQQQLAAQFYGAGGAPGLSGGMLGGTVQQPPPGLKSAPTPPAPGMSGIPMGGLHGLGSQFSGLGLGGHHNKSESSDALLRELMRSNGAGALGNTGLGGRAGDGKRMHVDSYWGGGSAKPSPVGDLADPSILQQQMQHHQGGIQAGQQGSGITPQERKLTPGMVDEDEFPALAPSTKENALALPPIASTLKPKKLPPGPIGKKKDESLEAAKDEHARPTQAKGKPVLPSVNTTAAAASSSGHRAISPAIVTPSTVSCEGTTPTSPAPPTAASAVKQGPRMIRILSSSSSIHSPAIRTPIGGGVGIGESDTGSNGGSSRPVSPPPNSQHSSKKGKTKSALKKERIAAARKEAEERERERERLERGSVTQAPVVGRMKKKGRKKDTTIGGGDPVAGGGYADEEKEEKEMEASNSATPQTVVAPTTVSLPSPGKEPITTTSIPAPTKAHTPAPVPTPAPTPAAIPAPVAPAPSTPTTPAVEQDGKQQSAKTTAQLLAEFQEAAKMKFSKLDMFKPLTTLKWEHYITSEEIQLIKTAVIENNNSQPILTVEPDCHPYQYPIRGGGVVNISLVVTPTGLQMTGLSAEQQERYLRLEERHGRGKAWQKWTVEALDFDECDKEVGDASVEELEKIVYASRKEVEGIEKKLEKLLKRNKKLVGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFQ6</accession>
    <name>D5GFQ6_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">Autophagy-related protein 13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00007009001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the ATG13 family. Fungi subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430220">
        <property type="protein sequence ID" value="CAZ83349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839158.1">
        <property type="nucleotide sequence ID" value="XM_002839112.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFQ6"/>
    <dbReference type="FunCoup" id="D5GFQ6">
        <property type="interactions" value="31"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GFQ6"/>
    <dbReference type="GeneID" id="9188297"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007009001"/>
    <dbReference type="eggNOG" id="KOG4573">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007151_1_0_1"/>
    <dbReference type="InParanoid" id="D5GFQ6"/>
    <dbReference type="OMA" id="FHQVGPT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990316">
        <property type="term" value="C:Atg1/ULK1 kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034497">
        <property type="term" value="P:protein localization to phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.900.10">
        <property type="entry name" value="HORMA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040182">
        <property type="entry name" value="ATG13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018731">
        <property type="entry name" value="Atg13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036570">
        <property type="entry name" value="HORMA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13430:SF4">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10033">
        <property type="entry name" value="ATG13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0072">Autophagy</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Autophagy-related protein 13 N-terminal" evidence="6">
        <location>
            <begin position="100"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="364"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="629"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="668"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="836"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="60"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="385"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="469"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="585"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="629"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="668"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="757"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="786"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="839"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="854"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="913"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="961"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="971"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="994"/>
            <end position="1011"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF10033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ83349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1011" mass="107507" checksum="472AA60131C23EC2" modified="2010-06-15" version="1">MYGRDSPQLPWDSPRRRTPAGGLAGGEIEQTRRFSTGFNYAGMDGSSTSSGSGGFTNVPPGGGHSRGASHGGYSSGASGASAGNNGGGERTNRDKVNQIIRAFFTKAAMIIIQTRAQTRPLTSKTGVKKVNKWFNIELDETDAFRRELDLWRYGDSFEPRPPPLIIETYLDTRDLTANQSLVILDADGKKWNVDEATDAAAARAGLNAEPRRSVRGGPARHEVILERWTVELGAPFDVALAPELPVVYKKGIVMFRALYTYAGLMPTWKFRRRLLRSKLNPSPLKVNCRVINGNDYSHTPKDFDLLYVPLCQGEGNVVETYQIEKVDSPAGSIKVRVSYRQNCDFRVDDSEALLSSQFLNLDEHFFRPPPAPRNPSQPSRREMSSLPSAGMNQPPRSEFNQTYGSLSSFHQQGTNPIGASPLSALRSAAEMADRASPTGSPIERPTTGLRSMHGSKASLRSVDGGGAQRRSSVSFMQPFKSPSLSASPANSEQVASSPRTSLNRVSAPVAPLHRQRPSLGAMPPSSGKMPPPSFEPISAQHAIPISNAGPSPLAAQPMTRITSSFGSRRPRIASGASRTDDDNNSSGKASYSSSAAPGSGLYADTGANSNEDLQIRDFMRLLANGQNQTLKSFGNSSGEASSKRTGNALSKYQKMRDSHISMADSISSSLLLQPSSPSPSTSSRHLSNVPAMMPSTEFSSSSLPGKPISPHTPHTPAIPSRLSAGLTAQHASRHGDQQGHSGRISLGSGDRPERTPSSRASSSSRLTTAATTSPLDIPSSPRPFGPRRSNSVSQQQQRLDSDSLGFDPLVPYGPEMRQSASLGSIDTAPLSMSRLLDLQNASATNLPGTSESGPEAREDNDRDDAPAFGPIRASQAEGDVPRFQQRRTTSHRGTAYSSSGSPGHSSRGRVLSRGRGSSTPPTTGSASSSVGGGTRPSGGRAFGYTKPGAYGEEDDLLFTMSDMSGAQQSRRSLDQERGGRDGSTGSSARRRGSRGGGAGSIGEGSRGAGWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFT6</accession>
    <name>D5GFT6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007068001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430220">
        <property type="protein sequence ID" value="CAZ83379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839188.1">
        <property type="nucleotide sequence ID" value="XM_002839142.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFT6"/>
    <dbReference type="GeneID" id="9187290"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007068001"/>
    <dbReference type="HOGENOM" id="CLU_1518952_0_0_1"/>
    <dbReference type="InParanoid" id="D5GFT6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072074" evidence="1">
        <location>
            <begin position="17"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="177" mass="19668" checksum="7F8269AA79D936B3" modified="2010-06-15" version="1">MKCILSILLFSLITLAAPVSQNFRLRILRTEGASGGPLSEYLGLKDGVAVITKNPSEIITAYLDPDPNGMLYTDKKIPGYLFPTDGTGTQVYDFRFGGIPKRADVLFTNFTFVDRICQVCMPDKILAYFQPKDDPSTRYEGQFRAYKDGEGWTLKFHASKPPSVEGSEGVDLIREAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>D5GG78</accession>
    <name>D5GG78_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00001990001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430244">
        <property type="protein sequence ID" value="CAZ83521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839330.1">
        <property type="nucleotide sequence ID" value="XM_002839284.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GG78"/>
    <dbReference type="GeneID" id="9184585"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001990001"/>
    <dbReference type="eggNOG" id="ENOG502RFG7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019422_1_1_1"/>
    <dbReference type="InParanoid" id="D5GG78"/>
    <dbReference type="OMA" id="HNANSAF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018961">
        <property type="entry name" value="DnaJ_homolog_subfam-C_membr-28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39394:SF1">
        <property type="entry name" value="DNAJ HOMOLOGUE SUBFAMILY C MEMBER 28 CONSERVED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39394">
        <property type="entry name" value="YALI0E31793P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09350">
        <property type="entry name" value="DJC28_CD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DnaJ homologue subfamily C member 28 conserved" evidence="2">
        <location>
            <begin position="247"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ83521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="512" mass="57726" checksum="D558CA09E221C350" modified="2010-06-15" version="1">MLLNRVILSRSTNIIHNSPRPFRSSLPTSSRVATACNSHPRRQASQSAESQDDTNSGGPPDAEEESALSRRFTEMTDEAMTSSPRKAAKITSENVLSEDLKRELEERIAKAQFNTTHAQALSVLDLPSGAPKQVRDIAAATPWTGTESIENAVLRMVVDTHKPPRGEPVRKPKPQTPGNVGPSPIRASKPQSASQRIAYAREKTTEYTLNKSDSLSEDEKSEVRNMFRERFMPEGRPVASLNAIASLADEKIEEARARGQFKNLPRGRPLERDHNADSPFLDTTEYFMNRIIQRQDIVPPWIEKQQDLAKAVRVFRERLRADWKRHVSRIISSAGGSIDEQCRRAEEYAAGEARLARIEDRAKKLERGEEVDPLDEDVTNRCSGTAVFRDPTWEKTELQYHKLAIGNLNSITRSYNLMAPELVKKPYFTLEREVKICFRDTAPLIPDVIRERAKISPQKVAEFQRGSNPGPLLEKFGASGNVVVYDSTKPNYGFKELFKDVFSGWKWGKQTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGE3</accession>
    <name>D5GGE3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00007338001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430264">
        <property type="protein sequence ID" value="CAZ83586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="KEGG" id="tml:GSTUM_00007338001"/>
    <dbReference type="HOGENOM" id="CLU_3436858_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGE3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ83586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="12" mass="1374" checksum="24B0241998E32378" modified="2010-06-15" version="1">MLMGGWKHDKNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGE6</accession>
    <name>D5GGE6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00007342001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CRP1/MDG1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430264">
        <property type="protein sequence ID" value="CAZ83589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839398.1">
        <property type="nucleotide sequence ID" value="XM_002839352.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGE6"/>
    <dbReference type="STRING" id="656061.D5GGE6"/>
    <dbReference type="GeneID" id="9183187"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007342001"/>
    <dbReference type="eggNOG" id="KOG1616">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011453_3_0_1"/>
    <dbReference type="InParanoid" id="D5GGE6"/>
    <dbReference type="OMA" id="WPHAGAS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031588">
        <property type="term" value="C:nucleotide-activated protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02859">
        <property type="entry name" value="E_set_AMPKbeta_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032640">
        <property type="entry name" value="AMPK1_CBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050827">
        <property type="entry name" value="CRP1_MDG1_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10343">
        <property type="entry name" value="5'-AMP-ACTIVATED PROTEIN KINASE , BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10343:SF81">
        <property type="entry name" value="CRUCIFORM DNA-RECOGNIZING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16561">
        <property type="entry name" value="AMPK1_CBM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="438"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="480"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-activated protein kinase glycogen-binding" evidence="4">
        <location>
            <begin position="5"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="140"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="285"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="178"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="205"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="327"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="347"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="372"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ83589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="525" mass="55308" checksum="6847BA5E1103D589" modified="2010-06-15" version="1">MAGSFTFKWAQPAGEVYVTGSFDDWGKSTKLTKEGDAFVATVSLPSEKILYKYVVDGNWTVDITARREAGDDGIENNVLLPEDLVKEKTGPDMSSAVISSAAPDSTTAVLAAQVPKETNGDVQDAAISSAAPDSTTAALAAGVSKEARTPSLKNVPGGFPQTPAIETSDPTYMVSPQEDAKKVTPSEPAPKHDDNSEDVGGGVKLDTRQEKADTSPEAPAVPEVAEQKIEVEEELKEGLSVVQPTTEGNTIIDQAQADIAAAARLSGASGEGNEETTPAITPEVAKQAQDATYVSPEATTAESTAEAKRELGESTTASAVSPASDAPEAKKAVENQLRDEVKPVTPAQLNNTAGDASTSPAPIPKLEVTDPQEATTPLSPTTPVEPAKNGPPGKSVENGNGEPPSSPTQLTRKKNRLSFFGSLKEKLHFGKDKGKSKGVVPVMIRKVHVLLFLIVFLLIRQTVIHTQRTMGREGKLGMRALWNSVSVIALFQSCMGTVSGFGSRVWGTIVPAVRSPLRFFHLSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGG8</accession>
    <name>D5GGG8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00007378001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430264">
        <property type="protein sequence ID" value="CAZ83611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839420.1">
        <property type="nucleotide sequence ID" value="XM_002839374.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGG8"/>
    <dbReference type="FunCoup" id="D5GGG8">
        <property type="interactions" value="57"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GGG8"/>
    <dbReference type="GeneID" id="9183209"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007378001"/>
    <dbReference type="eggNOG" id="ENOG502QT50">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079101_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGG8"/>
    <dbReference type="OMA" id="LWMDMVE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007667">
        <property type="entry name" value="Hypoxia_induced_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040153">
        <property type="entry name" value="Rcf2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28018">
        <property type="entry name" value="RESPIRATORY SUPERCOMPLEX FACTOR 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28018:SF3">
        <property type="entry name" value="RESPIRATORY SUPERCOMPLEX FACTOR 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04588">
        <property type="entry name" value="HIG_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51503">
        <property type="entry name" value="HIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="117"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="HIG1" evidence="6">
        <location>
            <begin position="89"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ83611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="247" mass="27382" checksum="5F231E3492E339D5" modified="2010-06-15" version="1">MKVLTKEQEGEHYAAVLKGGISGGLVGLGVGLGAAAVLNRRWQLFRSLTLPLKAFFVSSSGTFAAIINADVYSRGYEASRHAELREYKDSAARALAMARANRTGWERFKDFGRENRYPIVTAGWVASMAVSLGLVSRNRYLTTSQKLVQARMYAQGLTLALLIASAGFEVADARSGKGKFETVLVVDPEDPKHRHMIEKRIHHESYSGEDLWKDMVESEERRLKEREAARARARARMVLAPAGHSCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGL9</accession>
    <name>D5GGL9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00007413001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430275">
        <property type="protein sequence ID" value="CAZ83641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839450.1">
        <property type="nucleotide sequence ID" value="XM_002839404.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGL9"/>
    <dbReference type="STRING" id="656061.D5GGL9"/>
    <dbReference type="GeneID" id="9185563"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007413001"/>
    <dbReference type="eggNOG" id="ENOG502QRPA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_531209_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGL9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046529">
        <property type="entry name" value="DUF6594"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34502">
        <property type="entry name" value="DUF6594 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20237">
        <property type="entry name" value="DUF6594"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="468"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="493"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="domain" description="DUF6594" evidence="3">
        <location>
            <begin position="255"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="189"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="392"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="54"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="200"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="231"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="430"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="448"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ83641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="513" mass="55915" checksum="30D33D874BB365EE" modified="2010-06-15" version="1">MASLEEDNATADQKSQLSPPVPPPKTPPGKSHRRNRSDASSSSRGKKRRQRAFSGSGISISHSSTGSGEHEDPTTPKSTRQPSPHIVPYNANVAHSPVYRMPVQYGYYNSGFPPAPPPSLPFPPTLPSRQSMGMSYQRQRVDFEGSYNGPWASGPVRIEGTRMGPKGLNSEYYEQGRQDIPQILISSEDDSEDGQWYSGSDDDQTEDGGSEDKGKGKGKGRAGENESEDIPQPQASQASQQPRRGSPPPSRRTGYPYVAETLTNHPSLALYRRFSTLNHLVLLHLQDEISELESILAELDTAEYLSNAGLGLRSRRVGSGGGAKRIQVMGAVAWKLREYHMALKNFADVADTLEAAREDEIMAYRKWMDSERPLVGPEGRFLESREDLISLRKTPKLDSPVVPTPPPQAAHSQSQPPPPQSQPQSHEDSPPEEPSEPMVEDPKSTAPEPIPKEPLPSPTPTLSLSVKIYATLISLGISAVGGWILEKKSEMQVPAALTIGACGGAMGVMWMFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGQ8</accession>
    <name>D5GGQ8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00007470001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430275">
        <property type="protein sequence ID" value="CAZ83680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839489.1">
        <property type="nucleotide sequence ID" value="XM_002839443.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGQ8"/>
    <dbReference type="FunCoup" id="D5GGQ8">
        <property type="interactions" value="7"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GGQ8"/>
    <dbReference type="GeneID" id="9185602"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007470001"/>
    <dbReference type="eggNOG" id="KOG3979">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009808_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGQ8"/>
    <dbReference type="OMA" id="CVWYFPL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000031">
        <property type="entry name" value="Calcofluor white hypersensitive protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019402">
        <property type="entry name" value="CWH43_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057315">
        <property type="entry name" value="Exo_endo_phos_PGAP2IP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051916">
        <property type="entry name" value="GPI-anchor_lipid_remodeler"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053912">
        <property type="entry name" value="PGAP2IP_TM_1nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053911">
        <property type="entry name" value="PGAP2IP_TM_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14859">
        <property type="entry name" value="CALCOFLUOR WHITE HYPERSENSITIVE PROTEIN PRECURSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14859:SF1">
        <property type="entry name" value="PGAP2-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23022">
        <property type="entry name" value="6TM_1st_PGAP2IP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23021">
        <property type="entry name" value="6TM_2nd_PGAP2IP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23226">
        <property type="entry name" value="Exo_endo_phos_PGAP2IP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10277">
        <property type="entry name" value="Frag1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="168"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="278"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="310"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="357"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="459"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="496"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="524"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="547"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="577"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="612"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="653"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="domain" description="CWH43-like N-terminal" evidence="2">
        <location>
            <begin position="16"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="PGAP2IP first transmembrane" evidence="4">
        <location>
            <begin position="282"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="PGAP2IP second transmembrane" evidence="3">
        <location>
            <begin position="455"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="domain" description="PGAP2IP C-terminal nuclease-like" evidence="5">
        <location>
            <begin position="685"/>
            <end position="922"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ83680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="951" mass="106125" checksum="F16DFCB3327B1E8B" modified="2010-06-15" version="1">MPKDKDGTVLVSMSGKWVSHTHTVTAYCGFLGALIVGMSLHFHKIVQNEHYGYPDEWFPSVSATIGDRYPERSLFQLFICLTSGPRFALVFLWYLLTARPGAFAPKFVASIGLIRSLLCGGWVYITSTDDHDYHDIFMISYLVFTLPWTLGCLALSPMNLTAIKYRKLFAGLFFGTLVPLIYFFIQHKVHKVAGAYTIYAFFEWSLILFDVGFDAVTALDFEGFEFRVVDVKSVSKGKNIALSDAVLERSKMDKKDIPQQFLTDTYSWNDVLDALADVYSGFMFWSILTSLGVTIWYFPLWHMGISGYEVLVMTTVSPFLLGIGPVRRIVVNHPRYVYLLSLLGLLAFVSKVPETRLFFVAAGVWFTTLAWTGSFFAERAQPGRLEAKVSAFSLGLIASSIAKFACRSNNPIWPIMNTENGGWNKTGLVLAVLAVLRSTRKGPVGELPLSPPNKGGSSIIPAIGLGGLLFGLHSLLSDSSTMITWVWDGYPVKGPLAVPHGAYALVAMGAGFLIGLLREDLARTWTLYGVGCTAAAIFHRSPGWTGYYGALVLALYLMALVPSFIQSATKHSPGKTFGLSFLVYNLLVLAHVWVVAYAFVPGGPLLRERTDLIMTATMLAIGVGVFATTSYGPKSARELRAEDFPSRRRFRSYAVYSLVGLELFAASIAYMRFPSFDYQPYHKEEKVLTAGIWTVHFALDNDMWSSERRIRDVIKDLEVDVIGLLESDLQRIIMGNRDMTQFLAEDLGMYADFGPGPNKHTWGAALLSKFPIVNSTHHLLPSPVGELAPAILATLDMYGEMVDVLVFHSGQEEDVLDRKLQSEGIAKLMGDSPRPLILLSYLVTKPKQGNYNTYVSETSRMRDIDSSDWDRWCEYILYRGLKRIGYARVSRSTITDTEIQLGKFVVGEPEGTDEIIPESEVPGGYRFPDMFKGQGVRGHKYHVFNEPRYYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5GH37</accession>
    <name>D5GH37_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00007688001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the CDC50/LEM3 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430297">
        <property type="protein sequence ID" value="CAZ83830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839639.1">
        <property type="nucleotide sequence ID" value="XM_002839593.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GH37"/>
    <dbReference type="FunCoup" id="D5GH37">
        <property type="interactions" value="460"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GH37"/>
    <dbReference type="GeneID" id="9185084"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007688001"/>
    <dbReference type="eggNOG" id="KOG2952">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025025_0_1_1"/>
    <dbReference type="InParanoid" id="D5GH37"/>
    <dbReference type="OMA" id="IPWSMFN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005045">
        <property type="entry name" value="CDC50/LEM3_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10926:SF0">
        <property type="entry name" value="CDC50, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10926">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03381">
        <property type="entry name" value="CDC50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015840">
        <property type="entry name" value="DUF284_TM_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="344"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR015840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ83830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="401" mass="45632" checksum="6625D5D15F708AC9" modified="2010-06-15" version="1">MASSVDDRGPEAPKKKKEKNRRPADTPFRQQRLKAWHPILTPRTALPLFFAVGIVFGPIGGLLLWASTQVQEIVIDYTRCIESTSGELTTIPSSAVRSSFTTPLDPKDLPRWSMHTRPAPYDPDEEERVCTIEFTIPNEMKAPVLMYYRLTNFYQNHRRYVISLDEQQLKGEVRSYEDLDGSEACAPLAGADKVPYYPCGLIANSMFNDSFTSPVRVQAEGNSQGEEYVMTNKGIAWGADRERYKKTHYRPDQVIPPRNWVKRFPEGYTEKNMPDIHEWEEFQVWMRTAGLPTFSKLALRNDTLAMPAGKYRVNVTYNFEVMNFTGTKSIMLSTRTVMGGRNPFLGIAYVVVAGLCVVLGTLFTARHYWKPRKLGDRKYLTWNNEPTGGSHVPTPVGQETQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHF3</accession>
    <name>D5GHF3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00007913001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430318">
        <property type="protein sequence ID" value="CAZ83946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839755.1">
        <property type="nucleotide sequence ID" value="XM_002839709.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHF3"/>
    <dbReference type="GeneID" id="9183621"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007913001"/>
    <dbReference type="HOGENOM" id="CLU_2724046_0_0_1"/>
    <dbReference type="InParanoid" id="D5GHF3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ83946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="72" mass="8313" checksum="067810752CD805DC" modified="2010-06-15" version="1">MPNLCQDFWTLLESTPSVPHNPNRTPSFLPSKSQHIINLQHQRDANSNSNKSDYASENTLLIIRIKKKRGKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHS4</accession>
    <name>D5GHS4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Succinate--CoA ligase [ADP-forming] subunit alpha, mitochondrial</fullName>
            <ecNumber evidence="6">6.2.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Succinyl-CoA synthetase subunit alpha</fullName>
            <shortName evidence="6">SCS-alpha</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00008015001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of ATP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and nucleotide specificity is provided by the beta subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>succinate + ATP + CoA = succinyl-CoA + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17661"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Heterodimer of an alpha and a beta subunit. Different beta subunits determine nucleotide specificity. Together with the ATP-specific beta subunit SUCLA2, forms an ADP-forming succinyl-CoA synthetase (A-SCS). Together with the GTP-specific beta subunit SUCLG2 forms a GDP-forming succinyl-CoA synthetase (G-SCS).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 8">Belongs to the succinate/malate CoA ligase alpha subunit family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.5" evidence="6"/>
    <dbReference type="EMBL" id="FN430320">
        <property type="protein sequence ID" value="CAZ84035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839844.1">
        <property type="nucleotide sequence ID" value="XM_002839798.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHS4"/>
    <dbReference type="FunCoup" id="D5GHS4">
        <property type="interactions" value="767"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GHS4"/>
    <dbReference type="GeneID" id="9183689"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008015001"/>
    <dbReference type="eggNOG" id="KOG1255">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052104_1_0_1"/>
    <dbReference type="InParanoid" id="D5GHS4"/>
    <dbReference type="OMA" id="VIICITE"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00999"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009361">
        <property type="term" value="C:succinate-CoA ligase complex (ADP-forming)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004776">
        <property type="term" value="F:succinate-CoA ligase (GDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000002">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.261:FF:000005">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit alpha, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01988">
        <property type="entry name" value="Succ_CoA_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017440">
        <property type="entry name" value="Cit_synth/succinyl-CoA_lig_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033847">
        <property type="entry name" value="Citrt_syn/SCS-alpha_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005810">
        <property type="entry name" value="CoA_lig_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="SUCC_ACL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004230">
        <property type="entry name" value="PRK05678.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01019">
        <property type="entry name" value="sucCoAalpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11117:SF2">
        <property type="entry name" value="SUCCINATE--COA LIGASE [ADP_GDP-FORMING] SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11117">
        <property type="entry name" value="SUCCINYL-COA LIGASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02629">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001553">
        <property type="entry name" value="SucCS_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01798">
        <property type="entry name" value="SCOASYNTHASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01216">
        <property type="entry name" value="SUCCINYL_COA_LIG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00399">
        <property type="entry name" value="SUCCINYL_COA_LIG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="CoA-binding" evidence="9">
        <location>
            <begin position="33"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="6 7">
        <location>
            <position position="279"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="46"/>
            <end position="49"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="125"/>
            <end position="127"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit beta</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00061754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001553-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ84035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="326" mass="33991" checksum="4F3F6A5BC89F3ABE" modified="2010-06-15" version="1">MLALKRLSTFRVASVTRGYSTSSGYASTAKNLLINADTKVIFQGFTGKQGTFHAQQAIEYGTNVVGGISPRKAGSQHLDRPVFGTVKDAISKVGADASAIFVPPPVAAKSIEEAIEAEIKLVVCITEGIPQNDMVRVVDMLKTQNKTRLVGPNCPGIISPGQCKIGIMPGFIHKRGRIGVVSRSGTLTYEAVNQTTQVGLGQSLVIGIGGDPFSGTSFTDALSVFLGDDETDGIIMIGEIGGSAEEDAADYLKANNPKNKPIVSFIAGISAPPGRRMGHAGAIVSGGKGGAESKISALEDAGAIVERSPASLGKSLYEQFVKRDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIA2</accession>
    <name>D5GIA2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00008363001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430324">
        <property type="protein sequence ID" value="CAZ84245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840054.1">
        <property type="nucleotide sequence ID" value="XM_002840008.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIA2"/>
    <dbReference type="STRING" id="656061.D5GIA2"/>
    <dbReference type="GeneID" id="9181963"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008363001"/>
    <dbReference type="eggNOG" id="KOG4361">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032998_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIA2"/>
    <dbReference type="OMA" id="HYGNQRI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.120">
        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036533">
        <property type="entry name" value="BAG_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003103">
        <property type="entry name" value="BAG_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02179">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00264">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63491">
        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51035">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BAG" evidence="2">
        <location>
            <begin position="183"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="159"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ84245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="266" mass="28883" checksum="C15A861DAC91A0A4" modified="2010-06-15" version="1">MRRFFGRPQGPPEEAYLVTDREVHKGPLISGNENPDPDRSDDYVMIKHGMKEYMVSYPVGTITSGIVTTGAVRDQCVRLTGVEPDRVSLSCGGKVLRDDAAPLISLGIGHAARILCVASKATSAPPAGSEPAVAVDSVDGAPPSKSKKKRSKKKTRPASPLPPSPTPSPQPEVKRLPLTPFQQIEAVWEGIVADIHPLVNNFLQSPPADVDKREETHRRLTETMMGELLKLDSVESGEPEVRARRKEVVRQIQGTFDSLDALLKSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIM2</accession>
    <name>D5GIM2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00008559001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430327">
        <property type="protein sequence ID" value="CAZ84365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840174.1">
        <property type="nucleotide sequence ID" value="XM_002840128.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIM2"/>
    <dbReference type="FunCoup" id="D5GIM2">
        <property type="interactions" value="605"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GIM2"/>
    <dbReference type="GeneID" id="9183299"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008559001"/>
    <dbReference type="eggNOG" id="KOG2411">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014330_2_1_1"/>
    <dbReference type="InParanoid" id="D5GIM2"/>
    <dbReference type="OMA" id="LCGWVDR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004815">
        <property type="term" value="F:aspartate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070146">
        <property type="term" value="P:mitochondrial aspartyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.30">
        <property type="entry name" value="GAD-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00044">
        <property type="entry name" value="Asp_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004524">
        <property type="entry name" value="Asp-tRNA-ligase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004115">
        <property type="entry name" value="GAD-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00459">
        <property type="entry name" value="aspS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001750">
        <property type="entry name" value="PRK00476.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594:SF5">
        <property type="entry name" value="ASPARTATE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594">
        <property type="entry name" value="ASPARTYL/LYSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="7">
        <location>
            <begin position="135"/>
            <end position="577"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ84365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="593" mass="67132" checksum="8DC627C4200E00B2" modified="2010-06-15" version="1">MGEEVVLHGYFGPVRKAGKFLYFSFFHDYSSDPPRPLKVVSVIDSLSPQITKAAHQTLGSLHRFTPVALRAIVYKCAPPKSPEKRKSDEEVELRVKEIIPLNSVSENLIYTAKTIFPPEKRHLQLRTTKELCIALRLRSRTASECRKFLEENDFLEVETPLLFKSTPEGAREFLVPTRKGDGTCYALPQSPQQYKQILMASGVPRYYQLAKCFRDEDLRADRQPEFTQLDLEMSFATSDDVMDLVEGLVIRIWKNVLDVCVGPGFKRVPYWWAMKNYGTDKPDFRYPNHRIKNLHEVLPYLAKGDRFVEGFAFYGNTPNYNMDHAIEFLEKFKRENLSTRDDNYVAYIAGAGNETQAYIEGGMELWRDADLANIHGALWVHRPGAIVVIKERRGYYSGGNTKLGLLRSALLNKAIEQKFISSRSDYAFVWVIDFPLFSPTSADDPGQSGRAPFSSTHHPFTAPIAEDIQLLGSSPHRVRAEHYDLVVNGTELGGGSRRIHEAALQKYILEKVLKMPREKLQQFDHLLEVLDSGCPPHAGIALGFDRLIAVMMGRDSIRDVIAFPKNSKGADVMVGSPSVVPDKELQAYGLRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIN5</accession>
    <name>D5GIN5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00008585001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat ESC family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430327">
        <property type="protein sequence ID" value="CAZ84378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840187.1">
        <property type="nucleotide sequence ID" value="XM_002840141.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIN5"/>
    <dbReference type="STRING" id="656061.D5GIN5"/>
    <dbReference type="GeneID" id="9183312"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008585001"/>
    <dbReference type="eggNOG" id="KOG1034">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025586_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIN5"/>
    <dbReference type="OMA" id="GRQVAWS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051243">
        <property type="entry name" value="PcG_WD-repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10253">
        <property type="entry name" value="POLYCOMB PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2 6" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="117"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="166"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="334"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="362"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="381"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ84378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="460" mass="49710" checksum="F28A70846EAA55FC" modified="2010-06-15" version="1">MSNRTLFHSVRTTIGPNRGTTKSATYYGVYFYPYTKPTDDPVFAVVGGPETIIARPHPTKGLEVIQYFLDDLRFNESLCTASWTKDLKTGDPLIAVGGQAGIIKILNVKTGKVTQTLSGHGDEIMEILTSPKSQKLLASASADSTVRIWSLDPAHTRQPCALICAGEGHRETILSIAFHSSGRYLLSGGMDHIVNLWVLPDLPDESAGGDKPITLMYPHFSTSMIHSNYIDCLAFHGDYILSKAARESKIVLWAIQNFTSRLPPPARDKAPTTHEWRATRSAFGGGFDRLLQFSIPDTEPFFMRFGIFARACADTFLAMGSTSGRVHMWNLQRTETCGKSPGDTGTRDGRDTTTPSERGSPAVTSADLNTTGLIAGSDRGPSTAVAAPPAPAAPSAKLPSIRGDALGDPLAVIKAHYTLEIPRIKTTIRHVAYSLSGEYMVCVGEGGNIVISRKDPTVKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIU1</accession>
    <name>D5GIU1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00008674001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430328">
        <property type="protein sequence ID" value="CAZ84434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840243.1">
        <property type="nucleotide sequence ID" value="XM_002840197.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIU1"/>
    <dbReference type="GeneID" id="9185652"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008674001"/>
    <dbReference type="eggNOG" id="KOG4679">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011715_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIU1"/>
    <dbReference type="OMA" id="GIWNTES"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047767">
        <property type="entry name" value="PSP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007557">
        <property type="entry name" value="PSP1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43830">
        <property type="entry name" value="PROTEIN PSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43830:SF3">
        <property type="entry name" value="PROTEIN PSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04468">
        <property type="entry name" value="PSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51411">
        <property type="entry name" value="PSP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PSP1 C-terminal" evidence="2">
        <location>
            <begin position="670"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="242"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="764"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="802"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="95"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="818"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="854"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="869"/>
            <end position="886"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ84434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="907" mass="97926" checksum="6AB25CD7F09F0786" modified="2010-06-15" version="1">MSDIPSASTPRLSDISSIDSPRNSLDQTPPAGTSGVNQATATAGPFNPLGKTSPPSLIDDSNKPRHASPDSDALVSSDDELEQKELSYFSQHKNSALSALSSRRPSYAAEFQNRPRNYSMVGGGPLSPTSSHPSAPQGDTTAWAAGISPGSVGPTAAGQSLAPIWGSIWNSEPSRKSPPKPLNVQHSAVVPGSTQSPLFVGSEALPSPTSMTPNTGDFPIPIPLQPQLRNYRSMSFSVGQLPRDLDERPRLSPPAIGNGPRPHPPSGLQHRPSRPSLLSEEQYAGQSSPLRSVFEAEDDDANNAELNRAREPSGLHAGYLQQTGGAGTIQQANFIRSRYESMRLRNRSASTASVPLAAGLVGLAPIGLNGGRDGERNFSGEEYESALADDDELDYHHFQAQQADGRRFSEAPQRSMSLMYSPAENQRLEKLRRQHWQTAGHFAGIESGSELLAKRNVSPPKYGENGLAGLANRDNFYLDEARLRQRQSFPVSKAPVPSSITPPGQLHLQHLQQAYTAPTRLPSPPLGIPHRGMMGIGSHHAQQPRSQQLLYFVTFKACRGDVFYVQEGTGLRVRAGDLVIVEADRGTDLGTVAAENITWQEAKELKEEHAKEQYTWLMMFASRRAPASGLHNGVNGNGTGFANGGATGAMPGAHGQMQGQDGATAELKPKMIKRLAQVHEIQTLRDKEANEAKAKRVCQQKVLEHRLPMEILDAEFQMDWKKLTFYYFADSYINFNSLVTDLFKVYKTRIWMSAMNPASFAHPAGLHPPGSGPPGSGPPGSGALGAIGESNELQNQIMHQSQYSGHNPSYSGYVHSNGPGSSAMSHRGQQISANFPPRHSPHASVAPHQDFSHLPQQQLNGYNNLPYINANSNGHHSQNVGNNLDSHPNGGNDAWMASLQSLSLGSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJE4</accession>
    <name>D5GJE4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 10">Lysophospholipase</fullName>
            <ecNumber evidence="2 10">3.1.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00008960001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>a 1-acyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15177"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.5"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the lysophospholipase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.5" evidence="2 10"/>
    <dbReference type="EMBL" id="FN430330">
        <property type="protein sequence ID" value="CAZ84637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840446.1">
        <property type="nucleotide sequence ID" value="XM_002840400.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJE4"/>
    <dbReference type="FunCoup" id="D5GJE4">
        <property type="interactions" value="77"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJE4"/>
    <dbReference type="GeneID" id="9182638"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008960001"/>
    <dbReference type="eggNOG" id="KOG1325">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014602_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJE4"/>
    <dbReference type="OMA" id="GPEQYSL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1090.10:FF:000010">
        <property type="entry name" value="Lysophospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002642">
        <property type="entry name" value="LysoPLipase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728">
        <property type="entry name" value="CYTOSOLIC PHOSPHOLIPASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728:SF33">
        <property type="entry name" value="LYSOPHOSPHOLIPASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01735">
        <property type="entry name" value="PLA2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00022">
        <property type="entry name" value="PLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51210">
        <property type="entry name" value="PLA2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 9 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9 10" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="6 9 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="PLA2c" evidence="11">
        <location>
            <begin position="1"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ84637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="544" mass="58451" checksum="7079DFFFBB2FAC28" modified="2010-06-15" version="1">MVDFDVGAFMSSYSPTIGIAFSGGGYRAMLSGAGEFKAMDSRTPGSTAVGNIGGLLQSAKYIAGLSGGSWLLGSVVINNFTTIAKLQAAGNVWDFENSILAPEGALHIVDTTLYYNDLNREVQDKDNAGFDVSLTDYWSRALSRQFVNYTDGGPGITYSSIAKTDRYKNAEMPFPIIVADGRNPGETIISSNATVYEYNPLEFGSFDPTLYAFTPMGYIGSNVTNGVPNESGVCARGFDNVGFVMGTSSSLFNVLITELSSTGLPGVLRKLIETTLNSISAADNDIADYSPNPFFAVNPDINPSAKTRDLTLVDGGLDGQNIPLHPLIQPIRKVDVIFAMDNSADTRSNWPNGTALIATYERSIKGSQSNGTLFPSVPDVNTFINLGLNNRPTFFGCDGRNITSDTNKFPPLVVYIPNSPYTFNSNISTNQMAYTASERDLMIENGYNIATRGNASFDENWPACVGCAIVHREQERKGQTQTAQCQKCFKDYCWDGTRNSAVPNDYEPTALVSAKSAGTKNGAKSWGTVIIAVLSGLVSAVLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJG0</accession>
    <name>D5GJG0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008984001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430330">
        <property type="protein sequence ID" value="CAZ84653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840462.1">
        <property type="nucleotide sequence ID" value="XM_002840416.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJG0"/>
    <dbReference type="STRING" id="656061.D5GJG0"/>
    <dbReference type="GeneID" id="9182654"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008984001"/>
    <dbReference type="eggNOG" id="ENOG502T1M3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_696749_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJG0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="396" mass="44902" checksum="40BBCA6F4F5FAEB6" modified="2010-06-15" version="1">MTSKQQHSKALSSIQSTGGRTSAQPQASSPPSPIPLPGVLTEATKSEKRLFAAVIFRTPETLASKARNPKGLPEDLQRREKDLIKALPPRLLQSRSTSGRIANRWACKVHEPLNGDLLEALFYKIRYEAWGTHLNTLVPVKNRDAQELVANLRIISAYWIPPGDYEKLYMASPPRDDMYQDTDCDGCLLAAIAGDIVCLEAVRIAIYSRKHRGGRLVPWLDAWIRVHGKDALHRIRERSETVGRIVRNTKRASFTTLERQIKKDKRERDKSFLDELKYRERKLKSSDPFSPEARSYWEANKPKGPSVDSHAIPQEGEPNPINHVNASGPNPPEITKQVPLGRRAHSFYGEHFNPFDVYYNSDDDPLPDGESGYTSEYSTIDVAWHYREIERNGGWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJL5</accession>
    <name>D5GJL5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00009066001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bS21 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430332">
        <property type="protein sequence ID" value="CAZ84708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840517.1">
        <property type="nucleotide sequence ID" value="XM_002840471.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJL5"/>
    <dbReference type="STRING" id="656061.D5GJL5"/>
    <dbReference type="GeneID" id="9186742"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009066001"/>
    <dbReference type="eggNOG" id="ENOG502SYGP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1278451_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJL5"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070124">
        <property type="term" value="P:mitochondrial translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052837">
        <property type="entry name" value="Mitoribosomal_bS21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001911">
        <property type="entry name" value="Ribosomal_bS21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41237">
        <property type="entry name" value="37S RIBOSOMAL PROTEIN MRP21, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41237:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN BS21M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01165">
        <property type="entry name" value="Ribosomal_S21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="36"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="95"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="42"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="110"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ84708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="216" mass="23915" checksum="69E6CA6CDBFCD684" modified="2010-06-15" version="1">MARVTSCTRLLSSSFRTNPVSRSTLRQFTYSAIRFEDKKTETGPQTPTTAQTPHVAATTDTSTPQTTTQSEESDQCATPAQSILQQLLDMAGATSLRPPSQRSFKGGSVLPTNIVSRNPPNALNVPPISAPSKELPRTGSTAGRTVEVTTDLSSALSRLRTIIAQNKLRADQREQRFHVRPSLKRKRLKSLRHRKRFKDAFKRLVGIAMDMKRKGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJN5</accession>
    <name>D5GJN5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00009087001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430332">
        <property type="protein sequence ID" value="CAZ84728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840537.1">
        <property type="nucleotide sequence ID" value="XM_002840491.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJN5"/>
    <dbReference type="STRING" id="656061.D5GJN5"/>
    <dbReference type="GeneID" id="9186762"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009087001"/>
    <dbReference type="eggNOG" id="KOG4173">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055660_1_0_1"/>
    <dbReference type="InParanoid" id="D5GJN5"/>
    <dbReference type="OMA" id="CMTPQKR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039258">
        <property type="entry name" value="ZNF511"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21354">
        <property type="entry name" value="ZINC FINGER PROTEIN 511"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21354:SF0">
        <property type="entry name" value="ZINC FINGER PROTEIN 511"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="203"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ84728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="278" mass="31298" checksum="52CC2471C413E877" modified="2010-06-15" version="1">MAKRVRSDSVSSATSPSWTIFTRPVDQEDTVSQDLSSKSSYSKHVCLDPLSSEQTPSISCFLPPGCSGEPFASHEQFETHYIQAHSNRCHECGRNFPSLRFLDLHIAENHDPLTELKRERGDKTYACFVEGCSRLCSSPQKRRMHLIDKHHFPKEYNWNIVKWGIDQCTSLLSRKRSLPQTFTEGETLTAGGRDRFRSANVHKGETKRPPKANGKPVAATGATVDETYQQVQKGEGSKNIQKAKNEEIDMDDIAFTMSSLKIVPTSIRFGRRGGGHRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5GK94</accession>
    <name>D5GK94_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Conserved oligomeric Golgi complex subunit 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Component of oligomeric Golgi complex 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00009434001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COG4 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430337">
        <property type="protein sequence ID" value="CAZ84937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840746.1">
        <property type="nucleotide sequence ID" value="XM_002840700.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GK94"/>
    <dbReference type="FunCoup" id="D5GK94">
        <property type="interactions" value="799"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GK94"/>
    <dbReference type="GeneID" id="9184866"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009434001"/>
    <dbReference type="eggNOG" id="KOG0412">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014853_3_0_1"/>
    <dbReference type="InParanoid" id="D5GK94"/>
    <dbReference type="OMA" id="RASECQQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048682">
        <property type="entry name" value="COG4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048684">
        <property type="entry name" value="COG4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013167">
        <property type="entry name" value="COG4_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048680">
        <property type="entry name" value="COG4_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24016">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24016:SF0">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20662">
        <property type="entry name" value="COG4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08318">
        <property type="entry name" value="COG4_m"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20663">
        <property type="entry name" value="COG4_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00762">
        <property type="entry name" value="Cog4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="COG4 transport protein middle alpha-helical bundle" evidence="10">
        <location>
            <begin position="197"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="360"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="684"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="19"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="360"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ84937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="846" mass="92861" checksum="5637AD94A826A5BF" modified="2010-06-15" version="1">MTTATNGRNHKPRDRNPYDSDDDSSNEDDFSPPPPDVNSCTTVEELRAALAHLTAQDACIDNKLRTLLLKQSELEHSLTRLDLLRAHLGTQVVAARTLSNTMLSPAATTAQRVSSSVKRLDIEQSRVRATLEVVEQVSELKACVLGVTGSMGAPQDWETAASFLHRAGKIPQEIIRGEFAEEIVPTAEVPDVPSVTLENAAESLCGLFLREFERAAEAADGEKVTRFFKLFPLIGRTDVGLEVYGRYVCQGVAARARDALAAKRQAVADGAGGLGDFFYANAVTRLFEHIANIVEQHGKLIERHYGEGRMGKVIERLQVEADTQGGIIIDTFTDERSIGRKLTDIKSYAFSFLVQSYMPSTRGPMGGPPRSGSPAPSGATHRNSEDEGVDVREVDMLLSEIGVMLGRWSLYCRFLARKCLVGAKTDILPPLAAPPIITNSALYRKVQGRLIVPFNAMMTFFCRRSVEKAFQLDESPSDLSLSLTSPASSSNPPYITSAVDDVMYIVNNLLQRALHTSQRALVSNAISTVGRVLGSDFVGMIQRKMQVECYPKSSSGIPGAPVPDDKILGFLVLLNNLDVASDYTHRLIGTFANASSNIPGVDNNLRVDASNHTVAPLEELFPFQADAAIVRDALKGMETAFEGKAGELINDGIMVFFNQVVKPKLRPLLTEAFRDVEYLVSPEDDENAYPRSNPDGEQEGISSESRDELVKLRFQAGWDTLITPYKRILTEKIFNRLLNTTASYFSKLLEKRIWGYAGRISELGAIRLERDVAGVVGVVVRGGLYGVRDTFARCSQICFVVNMEEDEVGGLFTERAGSGGEDGDGVEWVLEVEERRRARGMVARKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKN0</accession>
    <name>D5GKN0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00009653001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430340">
        <property type="protein sequence ID" value="CAZ85073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840882.1">
        <property type="nucleotide sequence ID" value="XM_002840836.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKN0"/>
    <dbReference type="GeneID" id="9184735"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009653001"/>
    <dbReference type="HOGENOM" id="CLU_3360023_0_0_1"/>
    <dbReference type="InParanoid" id="D5GKN0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="36" mass="3950" checksum="4ECDFEA96A54750A" modified="2010-06-15" version="1">MSGYQTLPMSREELNTILGSAAPRRGAEGWGRERGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GL68</accession>
    <name>D5GL68_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">AP complex subunit sigma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00010029001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Clathrin-coated vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the adaptor complexes small subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430346">
        <property type="protein sequence ID" value="CAZ85261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841070.1">
        <property type="nucleotide sequence ID" value="XM_002841024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GL68"/>
    <dbReference type="FunCoup" id="D5GL68">
        <property type="interactions" value="377"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GL68"/>
    <dbReference type="GeneID" id="9182055"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010029001"/>
    <dbReference type="eggNOG" id="KOG0934">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061221_0_0_1"/>
    <dbReference type="InParanoid" id="D5GL68"/>
    <dbReference type="OMA" id="WYVATSE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030121">
        <property type="term" value="C:AP-1 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035615">
        <property type="term" value="F:clathrin adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016482">
        <property type="term" value="P:cytosolic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14831">
        <property type="entry name" value="AP1_sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000007">
        <property type="entry name" value="AP complex subunit sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044733">
        <property type="entry name" value="AP1_sigma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016635">
        <property type="entry name" value="AP_complex_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11753">
        <property type="entry name" value="ADAPTOR COMPLEXES SMALL SUBUNIT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015588">
        <property type="entry name" value="AP_complex_sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AP complex mu/sigma subunit" evidence="10">
        <location>
            <begin position="3"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR015588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ85261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="157" mass="18358" checksum="8535642C06F3DFE0" modified="2010-06-15" version="1">MAIRYLILLSRQGKVRLAKWFTTLSSKDKSKTIKDVIQLVLSRRTKMCNFLEYKDTKVVYRRYASLFFVAGIDSSDNELITLEIVHRYVEQMDKYYGNVCELDIIFNFQKAYYILDELLIAGEMQESSKRGLLKYIAQQDAIEDTEGEEDNISRIIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLL2</accession>
    <name>D5GLL2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00010256001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. KIN-14 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430349">
        <property type="protein sequence ID" value="CAZ85405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841214.1">
        <property type="nucleotide sequence ID" value="XM_002841168.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLL2"/>
    <dbReference type="FunCoup" id="D5GLL2">
        <property type="interactions" value="300"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GLL2"/>
    <dbReference type="GeneID" id="9182708"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010256001"/>
    <dbReference type="eggNOG" id="KOG0239">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001485_12_1_1"/>
    <dbReference type="InParanoid" id="D5GLL2"/>
    <dbReference type="OMA" id="RHDMQKC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01366">
        <property type="entry name" value="KISc_C_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47972">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KLP-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47972:SF45">
        <property type="entry name" value="PROTEIN CLARET SEGREGATIONAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 7" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5 6 7" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="10">
        <location>
            <begin position="320"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="155"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="375"/>
            <end position="382"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ85405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="630" mass="71044" checksum="49BB2B46A3A5375D" modified="2010-06-15" version="1">MSKGSAIPTGARPASAQGFRHPGGGESALESGAKSVMKRKHTDIKGRLEDTEAAIAEMREIQAQERVETMALKEQLEDHKLRAYQLETTRVTLETQNTQAQFELELYQKKTSELERILGDERMARAVERDDASKRLRDETDEIRRRLRDEMDGIIRSHREALETMERRARNDLEDEKSARAREIQELKMQAAMEKQRMELEMDGSGRDLRAIKAELETTRMELENARNELQREKVMSASLRDSLSEQSGATLTLESSSRALRARVEVLESDGRAQANNYAELEEKLRQSYEETRVAQEKLRAEETLRRKLHNQVQELKGNIRVFCRVRPTLPSESEMPADIRFPNAAHNPDVFEEISQLVQSALDGYNVCIFCYGQTGSGKTYTMSSEDGMIPRAVHQIYATAKELGEKGWSYKMEGSFVEVYNENINDLLGQADDFDKKKHEIRHDPKELKTTITDINTVTLDNPAKVESILRRASQTRSVAATKANERSSRSHSVFILKLIGLNSVTGERSEGTLNLVDLAGSERLSHSQSTGERLKETQNINRSLSCLGDVIAALGNGKENSHIPYRNSKLTYLLQYSLGGNSKCLMFVMVSPMQAHLNETLTSLKFATKVNNTSIGTAKRQAKITG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLN5</accession>
    <name>D5GLN5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00010286001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430350">
        <property type="protein sequence ID" value="CAZ85428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841237.1">
        <property type="nucleotide sequence ID" value="XM_002841191.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLN5"/>
    <dbReference type="STRING" id="656061.D5GLN5"/>
    <dbReference type="GeneID" id="9182731"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010286001"/>
    <dbReference type="HOGENOM" id="CLU_928102_0_0_1"/>
    <dbReference type="InParanoid" id="D5GLN5"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ85428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="300" mass="35175" checksum="18F81C68C0001BF6" modified="2010-06-15" version="1">MSQLRDSQDSYQYCERPHDVLALRRHIITNSEDRFFHHSTFSEANRTTWLTTLSFYQVHRSRPRKDSVPPAETGVFTSGFPTHDGDDKYIRQDKLTFSFTSISGDWENVHLSPRERNFWQLIAINPPRTHCLQNDIAGISTQIGPLSLRSWVESCLDGIREKWSEVLNELDRQISVTNSVLFEEEERIKFLFDDENFNNSKRYFWALQSLRVFSETMEETIQLLPSMFEKFYILGVNTQRRESGSDIQAVLTMEELNREMCDIAAKHAAKFQKIRNRIGRKRQEVKGLSDGLSETPSRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLR6</accession>
    <name>D5GLR6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00010371001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430351">
        <property type="protein sequence ID" value="CAZ85483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841292.1">
        <property type="nucleotide sequence ID" value="XM_002841246.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLR6"/>
    <dbReference type="STRING" id="656061.D5GLR6"/>
    <dbReference type="GeneID" id="9186467"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010371001"/>
    <dbReference type="eggNOG" id="ENOG502QVPF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_298627_0_0_1"/>
    <dbReference type="InParanoid" id="D5GLR6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050613">
        <property type="entry name" value="Sec_Metabolite_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001">
        <property type="entry name" value="UNCHARACTERIZED TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001:SF79">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Xylanolytic transcriptional activator regulatory" evidence="4">
        <location>
            <begin position="473"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="135"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="270"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="860"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="19"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="135"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="151"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="275"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ85483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1006" mass="110307" checksum="1853825EF1170B69" modified="2010-06-15" version="1">MKRQRTREDSEGADGETSEGYSTVGVTAGTNGPEFTSFARGKPEDLEARMNRLAEIVDRWYKDAYGQRIIPPVARQQAVDAVGVNNSDAKSSFEGTLGQIPTRERLVPQEQGVSDNMRKLTYSISGISCAPAHSIPCSSVSSSHSPRSRDTITTPSSSADGYSQQVCTLEGKNGATHPPKQMARGQLAMKLLDEEIEAARGKGTCDDIGDLGLGHLSIQGRGRSRYVGSSFWASISHEIAELNDVLRANEPPDTRCSAGPPPEEMIGLFQKPRNSTTSETASRSTASGTAEPLRPSSTASAPNFCGLSQFDKSSLLQFEPRERGGNPFEIIPLILRDLPDKPMCDIMYKAFLNGVHPIMPLIHVPTFAKEYNAFWAWFPNNIRSIPDPTLTENHGFFPLLLSVLYAGSMSLPAKVIEKILTGQSKIEVTKSLYSGSMHALSASSFPRTPTVSSLIAFLITQTCLIREEEPLTSCSFLGMAMRVAQSMGLHRDGSLFGLNEIECEVRRRVWWHILYLDVQGAIATGLPPLGGSGEELFDTKMVSELKDEYIGLSPEERKKVTEAATAAAEPQTSVNHMAHTCKALLDEHPNSPAMILAAGRYDMTIVLRKVVIQLLSTRPPQKADLMEMGGAIFELKLRLEDRIARIPARGIPEMGFTPPHDPEFHELMGHNESFFDTEKAIVFNGWARIMLSMFSDRTFGVFYQSFLKRSKSRLWDHARHCALHFCVSFLQKFVHVSKNPLFQPYQWFCPGTFQPLHATMILLIDLHERPQSPEAPQSRMFVDEIFALFGEDGNSLVGAPGGNRPLLEGGSLAWKMLARLRKKAYEKAGIKIDKPAERREEEGMATPTAPAKARSIAKCNMPPNPDTLTTPAAKDAGGKPGPDVLPPLDIPTGGYEEMLFIADNVATGTGRYKDTTEAAMKIWEAMAAAGIAVDDDAPMDPGMPTPPLMEPPLKELPMGVPTGDVAGTGADDVMDFDWEEWDAMFGRFTAIEGMVLSDMPVQRGPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLS1</accession>
    <name>D5GLS1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00010377001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430351">
        <property type="protein sequence ID" value="CAZ85488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841297.1">
        <property type="nucleotide sequence ID" value="XM_002841251.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLS1"/>
    <dbReference type="FunCoup" id="D5GLS1">
        <property type="interactions" value="664"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GLS1"/>
    <dbReference type="GeneID" id="9186472"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010377001"/>
    <dbReference type="eggNOG" id="KOG0671">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_5_12_1"/>
    <dbReference type="InParanoid" id="D5GLS1"/>
    <dbReference type="OMA" id="YIVQENS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043484">
        <property type="term" value="P:regulation of RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14134">
        <property type="entry name" value="PKc_CLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051175">
        <property type="entry name" value="CLK_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45646">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE DOA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45646:SF11">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE DOA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="312"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="55"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="238"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="55"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="120"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="172"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="249"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="341"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ85488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="662" mass="73476" checksum="84A468FE61686620" modified="2010-06-15" version="1">MSTPSTASAVLPPLSHYRYDYTRDHHRVLHPPPHAHLLNRTHLASPIYHSPVPVTTASTPTNSSFSSSTISAMASSRTQPSVKRNRNRTPNWEDFYKNGLPKEVIVIDDSPPPESGHLGANTNSKHSQPSSSSRVVAANGHQYPSTNNIAGGAYGPRHADKKRKVQTSQYEPSYTSQHQHSYSSTQTPHANFGSPTTISATSSTVSVNGNSTAGTSLGSNVGAGPSYSGASLASINGQKRKRVTRASQAAAAAANNGLDPYSSYHPPPRPPIKAKDVYVQPIPDPGRHPTGRVDDDDGHYIINLETDLTPRYRITKQLGQGTFGKVVEAYDRVKKTKCAIKVIRSVQKYRDASRIELRVLATLSCNDKENRNKCIHLRDCFDYRNHICIVTDLLGMSVFDFLKGNSFAPFPNSQIQSFARQLLTSVAFLHDLNLIHTDLKPENILLGRTRRVLLDTDIRLIDFGSATFDDEYHSSVVSTRHYRAPEIILGLGWSFPCDLWSIGCILVEFYTGDALFQTHDNLEHLAMMQAVVGQKIDTRLIRAVAGTGRTAVSNAVSRYFKGTRLDYPNDETTRASKKYVHAMKPLPDIIQPTTNFNKQFFDLLKRIFVYDPNKRITAKEALKHPWFREVVQDEGTEATKLRLERQAERLAAAATLSRGEGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNU0</accession>
    <name>D5GNU0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00011479001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430368">
        <property type="protein sequence ID" value="CAZ86183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841992.1">
        <property type="nucleotide sequence ID" value="XM_002841946.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNU0"/>
    <dbReference type="STRING" id="656061.D5GNU0"/>
    <dbReference type="GeneID" id="9182818"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011479001"/>
    <dbReference type="HOGENOM" id="CLU_585517_0_0_1"/>
    <dbReference type="InParanoid" id="D5GNU0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053209">
        <property type="entry name" value="Gramillin-biosynth_MTr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47643">
        <property type="entry name" value="TPR DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_5G12710)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47643:SF2">
        <property type="entry name" value="TPR DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_5G12710)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ86183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="467" mass="52797" checksum="57956BD91AEB08D7" modified="2010-06-15" version="1">MMRTTCPAIYGSVGSPAGSIISKQVKTKLNLSFSPPSSFSQSPQQIDENTMLTFIINTSLGILATWAFYKTTIVLLFPENHAEISILALLRLLVRDFLASPGPSVLPLDQLSYTLLEDARKSGRLRGGYFVLRTENGVWSDEKGTSCILEIQNNWRKNNWRNMEGEWYALIKEPFVRFQTREDGEHVVALMVRHRSDWDAIPSTSNLIPEGFRVVQGWREKGREELKEKGNDLYTQESYWGAFDLYKQALHQPEDSVPSDLIHRNCAQAAFKLSLRDTAIQDATAAISANPSDPKNSLKLAKCHYAARNYAEAETNLQKLVTLDPLNVKGKLLLERCHQRIAETRGEYNWVKIRKPLNTRPSSYLDVADYTGPVEVKVGKFGKGLYTTKNVKTGDLLLVSKALQFVYLSDGDLGNSEKLKDMKLDCILEVAERLHNEKGLQKGFFELWRGKEGVEPRIIDDTYALDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5GP21</accession>
    <name>D5GP21_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Arp2/3 complex 20 kDa</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00011629001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Functions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the mother actin filament.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ARPC4 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430371">
        <property type="protein sequence ID" value="CAZ86264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842073.1">
        <property type="nucleotide sequence ID" value="XM_002842027.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GP21"/>
    <dbReference type="FunCoup" id="D5GP21">
        <property type="interactions" value="696"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GP21"/>
    <dbReference type="GeneID" id="9183557"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011629001"/>
    <dbReference type="eggNOG" id="KOG1876">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084855_0_1_1"/>
    <dbReference type="InParanoid" id="D5GP21"/>
    <dbReference type="OMA" id="EAYLGEF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044396">
        <property type="term" value="P:actin cortical patch organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030041">
        <property type="term" value="P:actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1460.20:FF:000001">
        <property type="entry name" value="Actin-related protein 2/3 complex subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1460.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034666">
        <property type="entry name" value="ARPC2/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008384">
        <property type="entry name" value="ARPC4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22629:SF0">
        <property type="entry name" value="ACTIN-RELATED PROTEIN 2_3 COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22629">
        <property type="entry name" value="ARP2/3 COMPLEX 20 KD SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05856">
        <property type="entry name" value="ARPC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039100">
        <property type="entry name" value="ARPC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69645">
        <property type="entry name" value="Arp2/3 complex subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00054835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00082270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ86264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="205" mass="23587" checksum="7970197CDDE8C595" modified="2010-06-15" version="1">MVSRCRFNQISGGGVRVQASNVSSTATFHENWENTKMSQSLRPYLKCVRSSLTAAITLSNFASQTAERHNVPEIEAAKSPEVLLNPLTVSRNENERVLIEPSVNSIRVSIKIKQADEIEHILVHKFTRFLTQRAENFFILRRKAVRGYDISFLITNFHTEDMLKHKLVDFIVHFMEEVDKEISEMKLFLNARARFVAEAFLTPFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPB5</accession>
    <name>D5GPB5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00011789001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430373">
        <property type="protein sequence ID" value="CAZ86380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPB5"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011789001"/>
    <dbReference type="HOGENOM" id="CLU_2265681_0_0_1"/>
    <dbReference type="InParanoid" id="D5GPB5"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ86380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="103" mass="11622" checksum="C24EB9E907304CA7" modified="2010-06-15" version="1">MRFSYLFFFFFGIKKFADFIPYGKFQGFSWNAADVVGGWEHNLRIARVESGVFTLGQWSTCSAGYPRWGLFVSLGGESKGEYHTSIRFGLVGSSVVDVIRLCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPD7</accession>
    <name>D5GPD7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00011685001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430373">
        <property type="protein sequence ID" value="CAZ86299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842108.1">
        <property type="nucleotide sequence ID" value="XM_002842062.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPD7"/>
    <dbReference type="GeneID" id="9186539"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011685001"/>
    <dbReference type="HOGENOM" id="CLU_2160252_0_0_1"/>
    <dbReference type="InParanoid" id="D5GPD7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072865" evidence="2">
        <location>
            <begin position="20"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ86299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="111" mass="12008" checksum="8D21E3D9D63745BC" modified="2010-06-15" version="1">MTLLLLGYLLIEVSAPICGERRPLHFVARKAREWVSLCVVGMGGKMWMLGADRAGLCWWGRRSRGGGILGALESGIDGSVNLSLLEGSPLNRGRHGTKTQDLGKEGRGTFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPF0</accession>
    <name>D5GPF0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00011804001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430374">
        <property type="protein sequence ID" value="CAZ86393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842202.1">
        <property type="nucleotide sequence ID" value="XM_002842156.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPF0"/>
    <dbReference type="FunCoup" id="D5GPF0">
        <property type="interactions" value="258"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GPF0"/>
    <dbReference type="GeneID" id="9186632"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011804001"/>
    <dbReference type="eggNOG" id="ENOG502QSAH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040959_2_1_1"/>
    <dbReference type="InParanoid" id="D5GPF0"/>
    <dbReference type="OMA" id="CIEMGLY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd24142">
        <property type="entry name" value="ACL4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="90"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="371"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="24"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="38"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="378"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ86393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="404" mass="44392" checksum="94CBF8FA664D8FEF" modified="2010-06-15" version="1">MAKTRSQKSKSKSKSKSGKRERSVLNSTGAFSTNPHASTKTPSTSNSNPPIARPDESLLIEATALLHENSSPDAALPLLKKYLETNPNSPQALDLLGEAHIELGDTEAAFAAFSQSASLDPEGQASPAGTGPEKFLWLAQLSSAETAVAYYERGVEIIKRWLSTTSTTSHPVVTAERGLAKKLVSALCGLAEIYMSDLCMSPDAEPRCERYVTEALLAVPESAVALQTLASVRISQQRVGDAVAALRRAFDGWRGLPPAHEDVPPYADRVNLSKLLIETGEYEVALEVLERLRDEDDQLPDLWYLGGWCLFLLGEVEREREGGGGEWEESWGTAREWLENCLLLYNALEWEDEGIRDHAKEILSNIKKELPERMDKGEDVEDGGEDGGEEGEWESDDDDDEMED</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPH7</accession>
    <name>D5GPH7_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 10">Chitin synthase</fullName>
            <ecNumber evidence="2 10">2.4.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00011849001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Polymerizes chitin, a structural polymer of the cell wall and septum, by transferring the sugar moiety of UDP-GlcNAc to the non-reducing end of the growing chitin polymer.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[(1-&gt;4)-N-acetyl-beta-D-glucosaminyl](n) + UDP-N-acetyl-alpha-D-glucosamine = [(1-&gt;4)-N-acetyl-beta-D-glucosaminyl](n+1) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16637"/>
            <dbReference type="Rhea" id="RHEA-COMP:9593"/>
            <dbReference type="Rhea" id="RHEA-COMP:9595"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17029"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="EC" id="2.4.1.16"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cell membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the chitin synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.16" evidence="2 10"/>
    <dbReference type="EMBL" id="FN430375">
        <property type="protein sequence ID" value="CAZ86420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842229.1">
        <property type="nucleotide sequence ID" value="XM_002842183.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPH7"/>
    <dbReference type="FunCoup" id="D5GPH7">
        <property type="interactions" value="76"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GPH7"/>
    <dbReference type="GeneID" id="9186660"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011849001"/>
    <dbReference type="eggNOG" id="KOG2571">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004760_3_2_1"/>
    <dbReference type="InParanoid" id="D5GPH7"/>
    <dbReference type="OMA" id="NTIMSWF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030428">
        <property type="term" value="C:cell septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045009">
        <property type="term" value="C:chitosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004100">
        <property type="term" value="F:chitin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030476">
        <property type="term" value="P:ascospore wall assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006031">
        <property type="term" value="P:chitin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000920">
        <property type="term" value="P:septum digestion after cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04190">
        <property type="entry name" value="Chitin_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004835">
        <property type="entry name" value="Chitin_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914">
        <property type="entry name" value="CHITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914:SF9">
        <property type="entry name" value="CHITIN SYNTHASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01644">
        <property type="entry name" value="Chitin_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 10" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="4 10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="312"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="352"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="387"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="418"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="470"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="597"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="636"/>
            <end position="662"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ86420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="668" mass="75606" checksum="B7F232EC2CF01E6E" modified="2010-06-15" version="1">MYNEDEILFARTMAGVFKNIHYLCSRNRSQTWGTDAWKKVVVCVVSDGRGKINPRTRSVLAGMGCYQEGIAKQKVNDKEVTAHIYEYTTQVGIDLKGDLVKLTPNGKQGPVQMLFCLKERNQKKINSHRWFFQAFGQVLDPNVCVLIDAGTKPGGSSIYHLWKAFDINPQCAGACGEIKAMLGPGGKYLLNPLVATQNFEYKMSNILDKPLESVFGFISVLPGAFSAYRYTALQNDAQDEGPLEKYFKGEKMHGGDAGIFTANMYLAEDRILCFELVAKRKANWVLQYVKSSSAETDVPDEMSELILQRRRWLNGSFFAAVYALVHMFDIWRSDHSMMRKLMFHVEFFYQTISMLFSWFAIGNFFLVFRILTVSLADPALNFPPGNVLSIVFEWLYLGTLLTCFVLSLGNTPQGTKKLYMTIVIFWAVLMVYLLFAAVFISVKSVTKELADNGGKFSASSIFQNQLFRDLLISLSSTYLLYFVASFMFFEPWHMFTSFVQYLLLSPSYINVLNVYAFCNTHDISWGTKGPDVPPPIKNGAVKTVDGKADMNVPTDDKDLDEQYEAEIKVLAEKFVPEPEKPNESQIQKDYYAGVRSCVVLVWMFSNFALAATILNSAGLDRISLDDEKSEKERSKIYLAIVLWTVAALSAFRFLGASWFLIIRMFRGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPV4</accession>
    <name>D5GPV4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">4a-hydroxytetrahydrobiopterin dehydratase</fullName>
            <ecNumber evidence="3">4.2.1.96</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">4-alpha-hydroxy-tetrahydropterin dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00012066001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(4aS,6R)-4a-hydroxy-L-erythro-5,6,7,8-tetrahydrobiopterin = (6R)-L-erythro-6,7-dihydrobiopterin + H2O</text>
            <dbReference type="Rhea" id="RHEA:11920"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15642"/>
            <dbReference type="ChEBI" id="CHEBI:43120"/>
            <dbReference type="EC" id="4.2.1.96"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the pterin-4-alpha-carbinolamine dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.96" evidence="3"/>
    <dbReference type="EMBL" id="FN430379">
        <property type="protein sequence ID" value="CAZ86556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842365.1">
        <property type="nucleotide sequence ID" value="XM_002842319.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPV4"/>
    <dbReference type="STRING" id="656061.D5GPV4"/>
    <dbReference type="GeneID" id="9182299"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012066001"/>
    <dbReference type="eggNOG" id="KOG4073">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081974_1_0_1"/>
    <dbReference type="InParanoid" id="D5GPV4"/>
    <dbReference type="OMA" id="EANEPQW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008124">
        <property type="term" value="F:4-alpha-hydroxytetrahydrobiopterin dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00488">
        <property type="entry name" value="PCD_DCoH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.20">
        <property type="entry name" value="Transcriptional coactivator/pterin dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036428">
        <property type="entry name" value="PCD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001533">
        <property type="entry name" value="Pterin_deHydtase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12599">
        <property type="entry name" value="PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12599:SF0">
        <property type="entry name" value="PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01329">
        <property type="entry name" value="Pterin_4a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55248">
        <property type="entry name" value="PCD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00030497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ86556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="133" mass="15114" checksum="D47EFC68DCC9EFB5" modified="2010-06-15" version="1">MSTPTFGKAQALSEANEPQWSLGTDEATKKEALCLISRGRWSLSATRMGIEREFKFKTFAKAMVRLFINQVADQCKVKKHHPEWENVYNKVAIRWTTHNPEGLSHKDVEMAKFCDDKGIELGETGEEKIERGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPZ7</accession>
    <name>D5GPZ7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00012111001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430380">
        <property type="protein sequence ID" value="CAZ86590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842399.1">
        <property type="nucleotide sequence ID" value="XM_002842353.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPZ7"/>
    <dbReference type="GeneID" id="9182333"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012111001"/>
    <dbReference type="HOGENOM" id="CLU_1518955_0_0_1"/>
    <dbReference type="InParanoid" id="D5GPZ7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ86590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="177" mass="19935" checksum="F78C605EDA1B42DD" modified="2010-06-15" version="1">MIFRRQIMILKSTGTYGSGLWVSSASGSELKYKSDKQRNVTEQSIPKSRISEADARVVVKKEERVTDNSHKFSRGWPLVFFCFCFFSLIPEVLWPASTSVLFSSCFSFENLLSLLTAVPLTSPAHPRQFPPSASFPLSTWWIVASYDRFALISAFSLTNILPITRLINDEAINGTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>D5GQ39</accession>
    <name>D5GQ39_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00012187001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430382">
        <property type="protein sequence ID" value="CAZ86632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842441.1">
        <property type="nucleotide sequence ID" value="XM_002842395.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GQ39"/>
    <dbReference type="STRING" id="656061.D5GQ39"/>
    <dbReference type="GeneID" id="9185212"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012187001"/>
    <dbReference type="eggNOG" id="ENOG502RJYG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003431_1_0_1"/>
    <dbReference type="InParanoid" id="D5GQ39"/>
    <dbReference type="OMA" id="VFEVPNM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42345:SF2">
        <property type="entry name" value="HELICASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42345">
        <property type="entry name" value="TPR_REGION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="474"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="494"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="506"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ86632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="933" mass="102617" checksum="A370D2058B38488A" modified="2010-06-15" version="1">MSREEGEGHEIVAAHNRRWHSRISKSSFLGAIRKTLGKYRYSSSTSRTHSRVPTPFTKPVPHVPTHQNDGRPDPTPLNNKLNQKGGPSIPITRDRPDQTSPTREENNPTWTAPDHNLNPEPTVLSQHEIQSLFSGAPVFAASGKKASGGLRPRVMFPFDEDAEDRRGLSDCPPIEHAAFSLCTSRPHRALSGYENTTDHGLVNEEPSMLSFQGIEPGTCGWEYFLKYPVSDSENRDEEEDDDVIAGGGRERETRRASLGNAKGGLRTVEMGYIFERLKELGEIYHETKSKLPNGRKPLQGGAQRGILEKYSSLELYTNLFTRLLYPPTRITTADLHDPYSLKVQIVALRETLAMEKLWLDFSIVGWRIRLGQILWGENPTVESPNNEDGERGAAPLGSNTEKVWLLLQILLGCELVVRMDAILSEANSGDSSVISMGLGNELLDESKTMGSRKVEWDIALARRWLENVKLVESEVGKEEKTEAPPTPAKKGWFSSRSSEPSPTDQSESEGGDDESTLGRSYDAFVWPRNHTRQLSGLLYFARRLQWPQIELLSAMLREKLSPPSSFSTPSQSMAVTPLDTPLSGAGGNGYFPPFVRPKRVRELPSSGISSDSSFGMGGRGSGFPKEMGFGGWLSRTYFSGLILPGEGLPHFLISTLLESDALAVEKLGFRANLYSGFQYDGMTWWSSSSTVGKVLAGYDGSREVGGWIGPCLGSGLGEVGIIDDGWVDAVMVQLDEEPRAKRPYAIKEESNPLGKGPKGSRAVLSSDFTMPRDEINEEENVILEGLVFKGVAREPGELAGELSRYEVELHFSILPLNEKLEVKLTYDVFFISSFPCLEPPASMNSVKTGHLLHKDFPYQSVAVDALTEESFKDRNSILVIEAGGVDGGSEVLARAWCSMIGTHAIIGRKGRTCLGCCVREASGLGIGVVIRIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>D5G438</accession>
    <name>D5G438_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00003935001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mL41 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835160.1">
        <property type="nucleotide sequence ID" value="XM_002835114.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G438"/>
    <dbReference type="FunCoup" id="D5G438">
        <property type="interactions" value="107"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G438"/>
    <dbReference type="GeneID" id="9182408"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003935001"/>
    <dbReference type="eggNOG" id="KOG4756">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131055_3_0_1"/>
    <dbReference type="InParanoid" id="D5G438"/>
    <dbReference type="OMA" id="YIIEWDK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019189">
        <property type="entry name" value="Ribosomal_mL41"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21338:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21338">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN L41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09809">
        <property type="entry name" value="MRP-L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ79281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="100" mass="11452" checksum="912AAB61E29DCC42" modified="2010-06-15" version="1">MQPTNLLHRAIRRLQLTTKQVNGGYYKGTRSGAMGRHTKHGQYIINWDKVRTYVVPDLKGFVLTPFVTRKMEPTRGEFPGSSPLDGKAYLENWKMVNGED</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4T4</accession>
    <name>D5G4T4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00000183001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429991">
        <property type="protein sequence ID" value="CAZ79527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835370.1">
        <property type="nucleotide sequence ID" value="XM_002835324.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4T4"/>
    <dbReference type="STRING" id="656061.D5G4T4"/>
    <dbReference type="GeneID" id="9183009"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000183001"/>
    <dbReference type="eggNOG" id="KOG0137">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016513_1_1_1"/>
    <dbReference type="InParanoid" id="D5G4T4"/>
    <dbReference type="OMA" id="WHEPLPA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.600">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052904">
        <property type="entry name" value="Acyl-CoA_dehydrogenase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041504">
        <property type="entry name" value="AidB_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42707:SF2">
        <property type="entry name" value="ACD11 DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42707">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18158">
        <property type="entry name" value="AidB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0274">FAD</keyword>
    <keyword evidence="2 4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Adaptive response protein AidB N-terminal" evidence="8">
        <location>
            <begin position="55"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="7">
        <location>
            <begin position="236"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="6">
        <location>
            <begin position="355"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="13"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ79527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="685" mass="75771" checksum="E735F8B1FD70D250" modified="2010-06-15" version="1">MSNSGKKSTFTGPPQSQHPSQHNQQQHPHQPQQQPNLRPSSGDTGFFQDAPRLGNQFEEDVGLKRVLSLYLPPALLKPLHKELDSLAVAALSPQVLHWVEDAEAHPPTLESHDAFANRSDTLRTSEGWRRLAELGAHQGIVAEGYEGRYGRIGQFAKYYIFSPSSALTTCPFAMTDGAARLLSQHIPHPATSIQSFFSLNCSLSSRREAEAKRQVFYNAHFRLISRDQSARWTSGQWMTERSGGSDVSRSETVAVYSPLAPSSQIEDGTEELGPWVIDGYKFFSSATDADMTILLARTPGGGGKPSAFYAPMRLANGERNGVRIMRLKRKLGTRALPTAELELKGMRAWMIGEEGEGIKQIATVLNITRVHNAVSAISFMRRGLAIAKAFSKVRVVARNRPLWTIPLHLRTLAHLELYQRATTHLAFFTIHLLSLSETSAAPSTPSPNASSPLHTPSTPHQLLRLLTPIAKALTAKLAMSTLSECAESLGGVGYCENEEPLNIARLLRDAQVLSIWEGTTNVLVTDLISSLKRTSRAGGEEQGWVPFNEFVEENLGSPATATTSLVGEVEAEILERCKVALWEEWKEFEKILETRSKEELTSGGRVVLWTLGWVLCGVLLIVDARRDGERTAVEFARRWVLEKAGIWGAWGEYGVLEAEVEKRARADCWAVFGEKLPEVKDRARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>D5G515</accession>
    <name>D5G515_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00000321001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429995">
        <property type="protein sequence ID" value="CAZ79608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835451.1">
        <property type="nucleotide sequence ID" value="XM_002835405.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G515"/>
    <dbReference type="GeneID" id="9186306"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000321001"/>
    <dbReference type="eggNOG" id="ENOG502SEXN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_694811_0_0_1"/>
    <dbReference type="InParanoid" id="D5G515"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072522" evidence="2">
        <location>
            <begin position="19"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="128"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="224"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="238"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="275"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ79608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="397" mass="39993" checksum="C25F169363D947EE" modified="2010-06-15" version="1">MHFSTSLLLSAFLTLGAAISEGVSGSRNHQYFHRRHARVDGVAPAEVMPAAVEPAVVEPAHVEPATVHGPPNNAVVDISTPPNNALITSASTIVHVTGTSIQTLVPTAIVTVVDDSTLLTSLSTPLATSGAKVAPTNGGTHELSSSYGVAPTSTSATAETVVSSTPPAKVTSSVVTTESIGVVTSITEGGSVFNCSSTSSLMPPTTLNTTSTSPSRGVNATTTPFSTITGNATIHPITSTPVTTNTTTNTTTTRKFSTKNSFTTTSTSIPTAARSSSTKTSSKHSTPALPSPSTTYKLTLATPSASAVASQSNGLDMGEFNKTKPCFDKKCEDPGSEMTFNKTVTFNVGSGGDGGIRLGRGPDVASAATGGRGGGTLRTGISVAVVVVGVSIALGVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5G583</accession>
    <name>D5G583_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tryptophan synthase</fullName>
            <ecNumber evidence="5">4.2.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00000358001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + L-tryptophan + H2O</text>
            <dbReference type="Rhea" id="RHEA:10532"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.2.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the TrpB family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the TrpA family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.20" evidence="5"/>
    <dbReference type="EMBL" id="FN429997">
        <property type="protein sequence ID" value="CAZ79676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835519.1">
        <property type="nucleotide sequence ID" value="XM_002835473.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G583"/>
    <dbReference type="STRING" id="656061.D5G583"/>
    <dbReference type="GeneID" id="9188095"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000358001"/>
    <dbReference type="eggNOG" id="KOG1395">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4175">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016734_1_2_1"/>
    <dbReference type="InParanoid" id="D5G583"/>
    <dbReference type="OMA" id="FRAWIVR"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00044"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004834">
        <property type="term" value="F:tryptophan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd04724">
        <property type="entry name" value="Tryptophan_synthase_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000001">
        <property type="entry name" value="Tryptophan synthase beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00131">
        <property type="entry name" value="Trp_synth_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006653">
        <property type="entry name" value="Trp_synth_b_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023026">
        <property type="entry name" value="Trp_synth_beta/beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018204">
        <property type="entry name" value="Trp_synthase_alpha_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002028">
        <property type="entry name" value="Trp_synthase_suA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00262">
        <property type="entry name" value="trpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48077:SF3">
        <property type="entry name" value="TRYPTOPHAN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48077">
        <property type="entry name" value="TRYPTOPHAN SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00290">
        <property type="entry name" value="Trp_syntA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00167">
        <property type="entry name" value="TRP_SYNTHASE_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00168">
        <property type="entry name" value="TRP_SYNTHASE_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="13">
        <location>
            <begin position="336"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ79676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="506" mass="55025" checksum="7E13B86A855A166C" modified="2010-06-15" version="1">MQTGGSDIIEIGLPFTDPIADGPTIQGANTIALENGMSTTAALKAVLNARGKGLKVPVLFMGYYNLVLSYGEQELVKDCREAGVNGFIICDLPPEKAVKFKRFCNNECLSYVPLIAPSTKPSRLKFLCSMADSFIYVVSRMGTTGASGTLSAGLGDLCRRVREASSDIPIAVSFGVSTRDHFVSAGQFSDGIVIGSQVINVISQSKPEEINEKVKEYCQIMVGRNGNDWTTHEVGIVEAIGEANEPLDAHPTRAIAEKVVEPGPGLIDHLNALNVNREADLQSLPSRFGEFGGQYVREPLMGCLAELEHGFVEANNDPTFWEEFQSNYPYMGRPGQLHLAENLTKKCGGANIWLKREDLNHTGSHKINNAIGQIIIAKRLGKTAIIAETGAGQHGVATATVCAKFGMKCTIYMGAEDVRRQALNVFRMKILGAQVVSVEAGSRTLRDAVNEALRSWVVNLSTTHYLIGSAIGPHPFPTIVRTFQSLAWEVDRTVQECFIPLQRILR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5C1</accession>
    <name>D5G5C1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Eukaryotic translation initiation factor 1A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Eukaryotic translation initiation factor 4C</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00004265001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 9">Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the eIF-1A family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835557.1">
        <property type="nucleotide sequence ID" value="XM_002835511.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5C1"/>
    <dbReference type="FunCoup" id="D5G5C1">
        <property type="interactions" value="736"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G5C1"/>
    <dbReference type="GeneID" id="9188133"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004265001"/>
    <dbReference type="eggNOG" id="KOG3403">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109098_0_1_1"/>
    <dbReference type="InParanoid" id="D5G5C1"/>
    <dbReference type="OMA" id="KMEDQEY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05793">
        <property type="entry name" value="S1_IF1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000071">
        <property type="entry name" value="Eukaryotic translation initiation factor 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00216">
        <property type="entry name" value="aIF_1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006196">
        <property type="entry name" value="RNA-binding_domain_S1_IF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001253">
        <property type="entry name" value="TIF_eIF-1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018104">
        <property type="entry name" value="TIF_eIF-1A_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00523">
        <property type="entry name" value="eIF-1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21668">
        <property type="entry name" value="EIF-1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01176">
        <property type="entry name" value="eIF-1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00652">
        <property type="entry name" value="eIF1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01262">
        <property type="entry name" value="IF1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50832">
        <property type="entry name" value="S1_IF1_TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7 9" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 7 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S1-like" evidence="11">
        <location>
            <begin position="22"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="16"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00032507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00067293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ79714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="145" mass="16510" checksum="A3995A79E9EE70C7" modified="2010-06-15" version="1">MPKNKGKGGKNRRRGKNENDNEKRELTFKEDGQEYAQVVKMLGNGRLEALCFDGEKRLAHIRGKMRKKVWINQGDIILLSLRDYQDEKGDVILKYTADEARSLKAYGELPENAKINETDTYGHEGADECNFEFDVEAGDIDIDAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5M7</accession>
    <name>D5G5M7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="11">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00001490001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430000">
        <property type="protein sequence ID" value="CAZ79820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835663.1">
        <property type="nucleotide sequence ID" value="XM_002835617.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5M7"/>
    <dbReference type="STRING" id="656061.D5G5M7"/>
    <dbReference type="GeneID" id="9187116"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001490001"/>
    <dbReference type="eggNOG" id="KOG0201">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_2_1_1"/>
    <dbReference type="InParanoid" id="D5G5M7"/>
    <dbReference type="OMA" id="FKEIERW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000022">
        <property type="entry name" value="Serine/threonine-protein kinase 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF10">
        <property type="entry name" value="FI20177P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="1"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="200"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="267"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="427"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="558"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="200"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="283"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="322"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="347"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="454"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="466"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="490"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="506"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="558"/>
            <end position="574"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ79820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="647" mass="72138" checksum="29030D428EE78AE4" modified="2010-06-15" version="1">MEYCSGGSCGDLMKPGVIAEDYITIIIRELLMGLEYLHSDNKLHRDIKAANVLLGSNGQVKLADFGVSGQLTATMTKKNTFVGTPFWMAPEVIKQSGYDQKADIWSLGITAIELALGEPPYSDIHPMKVLFLIPKNPPPVLEGNFSDKFKEFVSQCLQRDPRDRPTAKDLLRHPFVRRAKKTTYLTELIERHERWVIENGNKDKNMDDDASSRMDSTIDSRSTDDSDAESDLWDFGTVRAVGGSRNPGLSSLSPAAANMRYVAQSVDQDYGEQARSKVGRRPVSPPMTGPPVPPRLPGNPSPTRDSLPPTTASQDSPPPYKFQQQNLQSQPISPPRRPNFTPAQTPRQREMSVDETDRSIQAELAQDMGWMKLARNQGPVNGIVSVPASIPMSKGITGEGRIEVELNSGVSTPTNAYRDPRQVFPPLHPPLHPPQMARQSSNDIAQEQMRRQMQHQQMQPHKMPAIGPPPGLHHPRLQQPPQPPSHHHINNGINHHQLPQLPPPQQHQLHQQHLPRQMTPNHKEATPEPPELTALDGVILPALEAALQRRTYHLQSAIRKAQQSSSASARQTAQEKQQAHDRLKRLVIKAAGIFREIEEWDSTAPVGMGGEVNGFLEGFLEEVLVRVEAEEDDGNNNVVREGENRRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5V0</accession>
    <name>D5G5V0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00001587001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430002">
        <property type="protein sequence ID" value="CAZ79893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835736.1">
        <property type="nucleotide sequence ID" value="XM_002835690.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5V0"/>
    <dbReference type="GeneID" id="9187571"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001587001"/>
    <dbReference type="eggNOG" id="ENOG502S40Z">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_953736_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5V0"/>
    <dbReference type="OMA" id="TENEAWH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="225"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="179"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ79893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="292" mass="33481" checksum="8BB4921A8BC5CA48" modified="2010-06-15" version="1">MRSNTGNLGSSKRHQILHTSPSLSLPFHLHRSREDTSRPKATAASPDVETYNYEYQANQHSASLPASRKGSLHHEMPERKISVPRNKPTVSNTGREELSCAITTLQTSSQKQMQRLDTTYSSVLQKLHELSGLASHLQELGKVAKSTVQEFDAADARCQSEFRSQIRVIERFKDELWNIELLRERLVAQKERVREYHERLDKVQERIDRHKEMEMVWKKRASRRLRLLWGFISILVILWLLATAGNDLETPLQDDPLRAPPQLAIPTPELARELLDISIVAPMLSDDALNEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>D5G653</accession>
    <name>D5G653_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="11">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00001775001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9">Binds 1 zinc ion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the peptidase M3 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430007">
        <property type="protein sequence ID" value="CAZ79996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835839.1">
        <property type="nucleotide sequence ID" value="XM_002835793.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G653"/>
    <dbReference type="FunCoup" id="D5G653">
        <property type="interactions" value="709"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G653"/>
    <dbReference type="MEROPS" id="M03.009"/>
    <dbReference type="GeneID" id="9187675"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001775001"/>
    <dbReference type="eggNOG" id="KOG2089">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001805_1_2_1"/>
    <dbReference type="InParanoid" id="D5G653"/>
    <dbReference type="OMA" id="KNFQSAM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006518">
        <property type="term" value="P:peptide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06455">
        <property type="entry name" value="M3A_TOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.40:FF:000001">
        <property type="entry name" value="Thimet oligopeptidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000006">
        <property type="entry name" value="Thimet oligopeptidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.40">
        <property type="entry name" value="Endopeptidase. Chain P, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1370.10">
        <property type="entry name" value="Neurolysin, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024077">
        <property type="entry name" value="Neurolysin/TOP_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024080">
        <property type="entry name" value="Neurolysin/TOP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045090">
        <property type="entry name" value="Pept_M3A_M3B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001567">
        <property type="entry name" value="Pept_M3A_M3B_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804">
        <property type="entry name" value="PROTEASE M3 THIMET OLIGOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804:SF84">
        <property type="entry name" value="SACCHAROLYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01432">
        <property type="entry name" value="Peptidase_M3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4 9" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M3A/M3B catalytic" evidence="10">
        <location>
            <begin position="224"/>
            <end position="688"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ79996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="691" mass="79228" checksum="9712C2C95635FE48" modified="2010-06-15" version="1">MTTGYPKPPPAPEFTHTPESVIEVTNRLIATSRQLQDNIAKDVTPETANFQNVLKPLAEDENEMGLDTAILGFYQYVSTNKGLRDASSEAERLLDDFGIESSMREDIYKLVSAINKSPQSKAVDVDAESSRFLEKVYESYVRNGLGLAEKERARFKEIKKELSILGIDFSKRLNEENGGIWFTPEELAGVPEDVLSGLSKGEPNTDNKGKLRLTFKYPDLFPTMKYAINTETRKKVFLGNENKCNENSKLFKKAIELRDEKARLLGFKNHAEFILQPKMAKNPERVLAFLTDLRKKLAPGGEKEKSRLKQLKAADTKDKKITNDGHYYLWDHRYYDRLLLEKEYKLDAQKIAEYFPLDHTIQGMMKIFELLFGLEFVEVTDETKNTWHEDCKQFRVYNEKPKDGSEQTFVGWLYLDLHPREGKYGHAANFNLQPGFTGKKGERHYPATALVCNFSKPTATKPSLLKHEEVVTLFHELGHGIHDLVSITYYSRFHGTNVVRDFVEAPSQMLENWCWTQSELTSLSSHYKTGEKIPQDMVKTIINTKHVNDALFNLRQLHFAFFDMEMHSYKNHGDAVKAEPSVRYNRLRTEIALLDPPNGVGDDFGHGEATFGHLMGGYDAGYYGYLWSQVFSTDMFYTAFKKAPMDGEQGRRYRHTVLEKGGSRDEMETLKEFLGREPNSDAFFEDLGLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5G678</accession>
    <name>D5G678_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glutathione S-transferase kappa</fullName>
            <ecNumber evidence="4">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00001646001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the GST superfamily. Kappa family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="4"/>
    <dbReference type="EMBL" id="FN430008">
        <property type="protein sequence ID" value="CAZ80021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835864.1">
        <property type="nucleotide sequence ID" value="XM_002835818.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G678"/>
    <dbReference type="STRING" id="656061.D5G678"/>
    <dbReference type="GeneID" id="9187700"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001646001"/>
    <dbReference type="eggNOG" id="ENOG502RH77">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069253_1_0_1"/>
    <dbReference type="InParanoid" id="D5G678"/>
    <dbReference type="OMA" id="PFWGFDR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004602">
        <property type="term" value="F:glutathione peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000096">
        <property type="entry name" value="Glutathione S-transferase kappa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001853">
        <property type="entry name" value="DSBA-like_thioredoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051924">
        <property type="entry name" value="GST_Kappa/NadH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014440">
        <property type="entry name" value="HCCAis_GSTk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42943">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE KAPPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42943:SF13">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE KAPPA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01323">
        <property type="entry name" value="DSBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006386">
        <property type="entry name" value="HCCAis_GSTk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DSBA-like thioredoxin" evidence="6">
        <location>
            <begin position="7"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR006386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR006386-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ80021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="225" mass="25311" checksum="6A80D6EBDD42E42A" modified="2010-06-15" version="1">MASIPRIKTYLDCVSPYSYYAFTFLNKHRAALDSHGVTIEFVPIFLGGVNVATGNQPPWKLPAKAAYVAIDMGNAKRYFGLPALKRPSVFPVPTVKPQRGLIYIKSHFPEETYVEAFRLLWVSLWEQDKDISQESQLVETWSGLFNEEELNSIIGATEEEEYKNALMENTNEAVKNGAFGAPWSVVRNAKGQETRYFGSDRFHFMWEFLGIGDGTGFAPSPKAVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6D7</accession>
    <name>D5G6D7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="13">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00004439001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430009">
        <property type="protein sequence ID" value="CAZ80080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835923.1">
        <property type="nucleotide sequence ID" value="XM_002835877.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6D7"/>
    <dbReference type="FunCoup" id="D5G6D7">
        <property type="interactions" value="304"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G6D7"/>
    <dbReference type="GeneID" id="9181165"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004439001"/>
    <dbReference type="eggNOG" id="KOG0667">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_88_1_1"/>
    <dbReference type="InParanoid" id="D5G6D7"/>
    <dbReference type="OMA" id="YRPTQTT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14212">
        <property type="entry name" value="PKc_YAK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000087">
        <property type="entry name" value="Dual specificity tyrosine-phosphorylation-regulated kinase 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000380">
        <property type="entry name" value="Serine/threonine-protein kinase ppk15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050494">
        <property type="entry name" value="Ser_Thr_dual-spec_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058:SF17">
        <property type="entry name" value="HOMEODOMAIN INTERACTING PROTEIN KINASE, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="357"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="695"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="762"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="841"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="122"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="157"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="202"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="228"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="715"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="762"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="789"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="802"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="860"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="386"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ80080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="902" mass="100855" checksum="E845CC08C418DA3B" modified="2010-06-15" version="1">MDPWPSYSTDSTANRQPRYANNVLQQNPSPQHLRDHNPPQQPPPSSMAAASGPGTTQQPYQAYHGTGAGPPPAQPAPTTRLDSASDYMDVQMPDADSYGRAKYQQPQSSRQPYVSPEEPASQRYTGIGSNSNPQAGYGSSASTHSLPQVNYPPQMATSTSSSSYQQRQSPSRLNYTSSSQSYYAQQQQPPAASPRTSAPPSMQSYQHHLQQSGSGSSGSDPNNGRYFSSSGSSMPQGSMYDQMHIGHHQSAPREHPPRFSRVAALSDLKPQTNSQPAFRRAHPEGGFISPLQALTTHLPSTYRICNPTFKYESSRNPRRVLTKPSKGVKNDGYDNEDSDYILYVNDILGSEETKNRYLILDVLGQGTFGQVVKCQNLKTQEVVAVKVVKNRTAYFNQSMMEVSVLDLLNSKLDKNDDHHILRLKDTFIHRQHLCLVFELLSVNLYELIKQNQFRGLSTTLVRVFAQQLLNALCLLNKARLIHCDLKPENILLKNLESPIIKVIDFGSACDERQTVYTYIQSRFYRSPEVLLGLPYSSSIDMWSLGCIVVELFLGLPLFPGSSEYNQVARINEMLGMPPVWMLEMGKQAGEFFEKVHDEFGRKIYQLKSMEKYSREHGTKEQPSKKYFQATTLPEIIRQYAMPRKNMKPIEIEREMNNRIAFIDFVRGLLNINPVERWSPQQAKLHPFITQQKYTGPFVPPMNLKSPPPKSPMSGAEQQRAEQAMMQKQQAAMQAQAQIQAQQRAQQAQQAAHVAQSQPNYSAVSLGNFPASSGHPQPPTVYNNAYSSTQQPNQPQQQAPPAYTTSTLYSQNPNRAARQRSATMVDGVPPQIQRAASMMDPTQPIRLQPSPAYFPPPPGGIPDEPGNGRGRQQARGGQYRNRNVIRTLEGNLEDGLLSQQHWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6F7</accession>
    <name>D5G6F7_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein HGH1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00004463001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HGH1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430009">
        <property type="protein sequence ID" value="CAZ80100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835943.1">
        <property type="nucleotide sequence ID" value="XM_002835897.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6F7"/>
    <dbReference type="FunCoup" id="D5G6F7">
        <property type="interactions" value="1033"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G6F7"/>
    <dbReference type="GeneID" id="9181185"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004463001"/>
    <dbReference type="eggNOG" id="KOG2973">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037769_2_1_1"/>
    <dbReference type="InParanoid" id="D5G6F7"/>
    <dbReference type="OMA" id="MCILLTN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061770">
        <property type="term" value="F:translation elongation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039717">
        <property type="entry name" value="Hgh1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007206">
        <property type="entry name" value="Protein_HGH1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007205">
        <property type="entry name" value="Protein_HGH1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13387">
        <property type="entry name" value="PROTEIN HGH1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13387:SF9">
        <property type="entry name" value="PROTEIN HGH1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04063">
        <property type="entry name" value="DUF383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04064">
        <property type="entry name" value="DUF384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein HGH1 N-terminal" evidence="3">
        <location>
            <begin position="97"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Protein HGH1 C-terminal" evidence="4">
        <location>
            <begin position="288"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ80100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="314" mass="35192" checksum="9BAB8CF69D1E3B49" modified="2010-06-15" version="1">MPTELEELVGFLGSPRPEIRQIALEHLVGYSQGPQNSIFKPEGLKPIKNLKVLVNDIAQHKPRAKNALKILINLSSDPPILTSLATDTPFLETLLSQITNLSNPNADLASMLLANLAKSDSIQSLITLTRPLLAKEVSASDNAMDQLLDLFVRGADKKLNEQACFDFLSYFFADVSRLGKGREYFVRRREYDGVVPISKVIVFTECGSLVRRKGVASAIKNSCFNIPSHKNFLAEDGINILPYILLPIMGPEEYPEDEMLEMPSEVQLLPPDKERESDKDIIVTHLETILLLTTTREAREYLRGVNVYCIVRER</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6H2</accession>
    <name>D5G6H2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00004482001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430009">
        <property type="protein sequence ID" value="CAZ80115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835958.1">
        <property type="nucleotide sequence ID" value="XM_002835912.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6H2"/>
    <dbReference type="GeneID" id="9181200"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004482001"/>
    <dbReference type="eggNOG" id="KOG1399">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_921712_0_0_1"/>
    <dbReference type="InParanoid" id="D5G6H2"/>
    <dbReference type="OMA" id="YAATHIY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050346">
        <property type="entry name" value="FMO-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23023">
        <property type="entry name" value="DIMETHYLANILINE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13738">
        <property type="entry name" value="Pyr_redox_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ80115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="318" mass="35499" checksum="950F997EFF901B77" modified="2010-06-15" version="1">MTSTYFDVIIVGAGPFGIIAAKTYLELNPTATLALLETDTSLGGAWSKNRVYPGMYSQSPLGMIEFSDVPMKPTPQHYKNYVPAHHVHEYLALYAATHIYDGKSIKDRIRLDTRVVSVQKLRSPRDGTGSESRRRGSTWVVGTDCGQTMVCSKLMIATGITSRPYIPFFNTLDFTPPMLHTKDLARLAPMFMESEGVRNVIVIGGSKSAFDAVHMFISAGKRVDWIIRDKGSGHAGVGLAERTPVYYKNSHQILSTRLMSKMSPCIFQPHDHWMHFFHQTRVGRWATDMVWNTMQFAWLKAARCGHSGDASGARPQLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6X9</accession>
    <name>D5G6X9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00002377001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430019">
        <property type="protein sequence ID" value="CAZ80272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842537.1">
        <property type="nucleotide sequence ID" value="XM_002842491.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6X9"/>
    <dbReference type="GeneID" id="9184086"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002377001"/>
    <dbReference type="HOGENOM" id="CLU_2401275_0_0_1"/>
    <dbReference type="InParanoid" id="D5G6X9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ80272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="93" mass="10611" checksum="09C25298DAACEECF" modified="2010-06-15" version="1">MIFMIPALAFLRTRQLRPGSSSTSLHLTRRLIDPLIAEHLSICLSPRRTELNLAQFDSIELNSVGKKEREIYIYIYLPAPWRFPGLNSAKISG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>D5G704</accession>
    <name>D5G704_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">GSTUM_00004530001</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
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            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
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        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
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        <property type="protein sequence ID" value="CAZ80297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_002836060.1"/>
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    <dbReference type="KEGG" id="tml:GSTUM_00004530001"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_52_1_1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:chromatin"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="F:protein serine kinase activity"/>
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        <property type="term" value="P:cellular response to oxidative stress"/>
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        <property type="term" value="P:positive regulation of hydrogen sulfide biosynthetic process"/>
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        <property type="term" value="P:regulation of cysteine metabolic process"/>
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    </dbReference>
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        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
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    <dbReference type="Gene3D" id="2.60.40.150">
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
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        <property type="entry name" value="Pkinase_C"/>
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        <property type="entry name" value="Protein_kinase_ATP_BS"/>
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        <property type="entry name" value="Ser/Thr_kinase_AS"/>
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        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
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    </dbReference>
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    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="C2" evidence="12">
        <location>
            <begin position="336"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="13">
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            <begin position="528"/>
            <end position="789"/>
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    <feature type="domain" description="AGC-kinase C-terminal" evidence="14">
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            <begin position="790"/>
            <end position="873"/>
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            <begin position="875"/>
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            <begin position="14"/>
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            <position position="557"/>
        </location>
        <ligand>
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    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51285"/>
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    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAZ80297.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="940" mass="102192" checksum="B42E37D80728FBA3" modified="2010-06-15" version="1">MVTAFQFFRGGGTSPASSAPGTPLLTSHVTTAQSSSTAPATPIAGPSPTTATALPELSCLSPLSRRQSPVLRTGSTPLGSSGNGTTTATSHSNAVASPRPQLPLNTAAAVSSSASNMLRAVAGQSALSLVSHLNNSLNLSPIFFCCNCSFGHLASVFCLVHEMWRAISNGLRILLLFNPQSWTDGPDIEHRSDDDENLSGQTTPHPSGLDKRLPGILHGFSQVCSELLPFLRRNTTPLEPRLPPQSRKTVSFESSSCHFLSASLSDMGLEGKESKRLTGDVFSDGKDTPPATPRGTASTGSTLSGSTAATTPSTSRPNSTSASNNNTTPMSPKPVSPGSGASSNAAAHAAVVPPKGKMVVKIIEARNLRPSKDPYVVCTFESNEFISKGPKKSDDSGDGSDNEKAALAPGRGIAIPMKSRQSSSTSLSELQGGGSKKGITNPKWEHEAVFDVLGEQSEIDVSIYDRSNQECFLGHVRMCPLATREQQSGLDNWFALQPRTHHESVAGEIRLQISFERISKKHYGPDDFEVLRLIGKGTFGQVYQVRKKDTKRIYAMKVLSKKVIVQKKEVAHTLGERNILVRTATTDSPFIVGLKFSFQTPTDLYLVTDYMSGGELFWHLQKEGRFKEDRAKFYIAELILALEHLHNHDIVYRDLKPENILLDANGHIALCDFGLSKANLSANATTNTFCGTTEYLAPEVLLDELGYTKMVDFWSLGVLVFEMCCGWSPFYAEDTQQMYKNIAFGKVRFPRDALSLEGRNFVKGLLNRNPRHRLGATLDAKELKSHPFFNDVDWNELLRKNVIPPFKPTLSSETDTSNFDPEFTNAPMSQSLNARAAALASGNLAASTPLSPGMQANFRGFTFVDESSIDDHFLGRDELARMDEGEEDGDWDERHDNRRNNDDEDDARGFDGRGNRMSGIVKTGQADDEAGIFVNDGMDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7S7</accession>
    <name>D5G7S7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00004707001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430031">
        <property type="protein sequence ID" value="CAZ80570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836379.1">
        <property type="nucleotide sequence ID" value="XM_002836333.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7S7"/>
    <dbReference type="STRING" id="656061.D5G7S7"/>
    <dbReference type="GeneID" id="9181389"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004707001"/>
    <dbReference type="eggNOG" id="KOG1270">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037990_1_0_1"/>
    <dbReference type="InParanoid" id="D5G7S7"/>
    <dbReference type="OMA" id="HHFDKPD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591:SF108">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase" evidence="2">
        <location>
            <begin position="55"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="174"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ80570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="272" mass="29859" checksum="33EB49060A15C165" modified="2010-06-15" version="1">MAQGSAEVNKQYFNDNADDYDARAEASNVANIIGENILLRREWSGAEWDEATTRVLDYACGTGLISRIIAPYTKQIIGMDISEKMVEKFNEKVNNQGIPEEEMGAVVADLCADELDPSLSNPEYHNFDVIVCGFAFHHFSDVQLATKRLVERLKPNTGVLLVIDFKSHPPPSDHITNHGGHAHHGRHQHPGHGGQGKRATEIKAQDTVAHGGFSQEDMRKVFEGTGLVEVDFVDVGPNGERGKAITFLGHGGGSGKDIQMEREVFMVKGKRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7T7</accession>
    <name>D5G7T7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00004719001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430031">
        <property type="protein sequence ID" value="CAZ80580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836389.1">
        <property type="nucleotide sequence ID" value="XM_002836343.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7T7"/>
    <dbReference type="GeneID" id="9181399"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004719001"/>
    <dbReference type="HOGENOM" id="CLU_880520_0_0_1"/>
    <dbReference type="InParanoid" id="D5G7T7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055936">
        <property type="entry name" value="DUF7514"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24355">
        <property type="entry name" value="DUF7514"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7514" evidence="2">
        <location>
            <begin position="102"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ80580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="316" mass="34324" checksum="336875EB4DA7FCB8" modified="2010-06-15" version="1">MLAQPRYPLLGPVLSAASAQTTTSAVAANSRLLLGLRRGAARNPSSLPTHQHLPGLSYHVPPALPPPLPPRSHNIEETSPYLRPTPPATRIPPHPPTAASKLLSSNNAPTRRLKDRSDGVFAYRLLNPGQISNFCELAGKPLKENPFGVRVNEADSTSSSPDNNTDAGVAHLYNRLGIEYSLITRVTEGVVSQVAINHMPVLGHRGFRQLPVRETLEDPGHAHSFFGRLVRPAIVDGRRAFGEVSWGMFPAELDPAFATTSELSARVGALVAGLGSGSWHSGRRRLRRRTGRVWGPSARSMIRIWLLCTILTSSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7W9</accession>
    <name>D5G7W9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00002685001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430032">
        <property type="protein sequence ID" value="CAZ80612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836421.1">
        <property type="nucleotide sequence ID" value="XM_002836375.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7W9"/>
    <dbReference type="GeneID" id="9181431"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002685001"/>
    <dbReference type="HOGENOM" id="CLU_2252020_0_0_1"/>
    <dbReference type="InParanoid" id="D5G7W9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="104" mass="11210" checksum="3D3E7DE9EE9E7B23" modified="2010-06-15" version="1">MEGQVGSRLGRCRGGVKGVRERGNGRPATCISLEREVGCCRETKKNLVIVMLQVLVPYSDSIMMKANVGKQASKGTLDRSAAMCSQSTRSHENSTVGISIWSAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5G803</accession>
    <name>D5G803_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00002767001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430037">
        <property type="protein sequence ID" value="CAZ80646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836455.1">
        <property type="nucleotide sequence ID" value="XM_002836409.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G803"/>
    <dbReference type="STRING" id="656061.D5G803"/>
    <dbReference type="GeneID" id="9184128"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002767001"/>
    <dbReference type="eggNOG" id="KOG1840">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_125_15_1"/>
    <dbReference type="InParanoid" id="D5G803"/>
    <dbReference type="OMA" id="QRTYMTD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053137">
        <property type="entry name" value="NLR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46082:SF6">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46082">
        <property type="entry name" value="ATP/GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13374">
        <property type="entry name" value="TPR_10"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ80646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="805" mass="90591" checksum="45F34C1B31EC0E37" modified="2010-06-15" version="1">MRRFISAGMRKIPVPDHPHSLRNIDNQGVALCHQGKYDEAEVMHRRALAGREKTLGPDHQDTLQSVVNLGAALHCQGKYDEAEIACRRALAGREKTLGLDHQDTLESVDNLGTTLHSQGKCNEAEIMHRRALAGREKMLGLDHQDTIQSINNLGTTLRRQGKHDEAEIMHRRALAGREKTLGPVHQDTLRSVGDLGTTLHRQGKHDEAEIMHRRALAGREKTLGLDHQDTLRSVSNLGTTLHRQGKHDEAEIMHRRALAGRERTFGLDHQDTLRNVNNLGTTLHEQGKHAEAEIIHRRALAGREKTLGRDHRDTLESVNNVGMTLRSQGRYNEAEIMYLRALAGREKTLGLVHRDTLQSVRDLGVALYRQEKYDEAGIMHRRAFAGREKMLGLDHRDSLLSSNNPSVVLHDRKKNNEMAIVHRHAAEVGENALGPHNPAVLLGAIGLAVTLLAERKYEEAEIVYRHALRTMEKALGPDHPGALDSMNLLGETLLDLERYGEAEAMCRRALAGRKKALGLHHAATLTSADNLGRTLYHQRKYEEAEMMFRSAQAGRKTLFGLYHPDTLLSILRLGVTLLDREKYEEAETVYRHALGVMEDALGPDHPEVLDSMNTLGETLLDLKKYSEAEVMCRRALVGREKTLGPDHRDTLLSVNNLGVALRNQNKHHEAEISYRLALMGTERTLGRDHRDTLTCAYNLAGALHCQKKYDEAELMHRRALTGREKILGLGHRHTLRSLVRLGTVLYEKREYEEAEIMYRRAATRAERALGPGHPTTRKTFNRLSMFLNAGPTSVMSTLDEASWQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5G823</accession>
    <name>D5G823_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00002721001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase PH family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430038">
        <property type="protein sequence ID" value="CAZ80666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836475.1">
        <property type="nucleotide sequence ID" value="XM_002836429.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G823"/>
    <dbReference type="FunCoup" id="D5G823">
        <property type="interactions" value="136"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G823"/>
    <dbReference type="GeneID" id="9184138"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002721001"/>
    <dbReference type="eggNOG" id="KOG1068">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063514_1_2_1"/>
    <dbReference type="InParanoid" id="D5G823"/>
    <dbReference type="OMA" id="WSAVEMT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071028">
        <property type="term" value="P:nuclear mRNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050080">
        <property type="entry name" value="RNase_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953:SF2">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT MTR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0271">Exosome</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="9">
        <location>
            <begin position="36"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ80666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="336" mass="35066" checksum="F2E1A3811A2F6626" modified="2010-06-15" version="1">MTDRRRINGPLEGTIPPVFLTSSTHKSIIKRSPTTLRKIFLKTGLTPPATGSAFLELPTPSTHSTPTLKLTSSVYGPRPLPSSTTFSSNARLTAELKFSPFSTPGRRRGYIRDGVERDLSAQLSIALGKSVAVGKYPKSAIDVFVSVLDCEGGLGDAGDEAGGGVDVGLMSVLATAISCASAAIADAGIECFDLVAGGVAGLVLEQGGVVEEDRVEKSLEVGQDSDDARRGMRKNGKVTLVLDPSPIDGYIILAAAAVGYMAARDELTLVWTKGSMGWDGDSTDEVERIVDGAITASTGVRLVINEAVKERLLLGLKEMGLIRGKAVSGDGDAAMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8F6</accession>
    <name>D5G8F6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00004801001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430042">
        <property type="protein sequence ID" value="CAZ80799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836608.1">
        <property type="nucleotide sequence ID" value="XM_002836562.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8F6"/>
    <dbReference type="STRING" id="656061.D5G8F6"/>
    <dbReference type="GeneID" id="9186019"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004801001"/>
    <dbReference type="eggNOG" id="ENOG502QU4J">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034224_0_1_1"/>
    <dbReference type="InParanoid" id="D5G8F6"/>
    <dbReference type="OMA" id="RIHLVHD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018814">
        <property type="entry name" value="DUF5427"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28265">
        <property type="entry name" value="MAINTENANCE OF TELOMERE CAPPING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28265:SF1">
        <property type="entry name" value="MAINTENANCE OF TELOMERE CAPPING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10310">
        <property type="entry name" value="DUF5427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="453"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="474" mass="50247" checksum="0759F06F8495340D" modified="2010-06-15" version="1">MAHRSGADLDELFEGIEDASTSRPPPSAQDEQDLLAGLESLAAGHKTSSRPHTPRVSTPSVRSRTPMGRNSGEGYSGYGIPPIGRDSGDVRRGGDNTPTLREKGSAVTPVNAAEPPASVSSMSSTGITTGGGGGGWGWGSIWETAASTATAAVKTAEGVVKELQQSEEGKKWAQQVKENAGALRGLAGDVKSRALPTFTTLLHTLAPPISSHEQLCIHITHDLENYPFVDTVVYGVFDRVMQQVEGGDLLVVQRGGGSKPRSSLDGAFGGPWYKGMERRDLHATVGLQEGIKLASVAAESYAQEFLQTSDAFTGPASKDNPTRKSNIFLAIQPTIHSQQPSPLAPRGEDSEEQEDQAVVFAIHLFDPTHGISYSTVSQALPTQWLEWLDAPPPADAAPGEWAVPDVIRNIIDIGGIDPREWIVEWVEEAVGLAVGVVAQRYVAKRMRIGDSGSHDGLKGKEVSSGGEEARAVAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8Q7</accession>
    <name>D5G8Q7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00003099001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430050">
        <property type="protein sequence ID" value="CAZ80900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836709.1">
        <property type="nucleotide sequence ID" value="XM_002836663.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8Q7"/>
    <dbReference type="GeneID" id="9186268"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003099001"/>
    <dbReference type="eggNOG" id="ENOG502QTW2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021105_1_2_1"/>
    <dbReference type="InParanoid" id="D5G8Q7"/>
    <dbReference type="OMA" id="HYYLLIG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041664">
        <property type="entry name" value="AAA_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056808">
        <property type="entry name" value="HTH_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36168">
        <property type="entry name" value="CHROMOSOME 1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36168:SF1">
        <property type="entry name" value="ORC1-LIKE AAA ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13191">
        <property type="entry name" value="AAA_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24913">
        <property type="entry name" value="WHD_AAA_fung"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Orc1-like AAA ATPase" evidence="2">
        <location>
            <begin position="203"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="AAA protein C-terminal winged helix" evidence="3">
        <location>
            <begin position="439"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ80900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="613" mass="68912" checksum="C2DFA7614F009213" modified="2010-06-15" version="1">MAMIIPRSFQRTRRSSLFSTKSPQHGLFLPPCILQQKRWSTNQPNSVGGGNDYQFGPSTDLQPQPSTQKWGLIEDMAALGGDQTGGTRGGGTHHGSPRADDGNRGSSRRGGGKPPGGEDDTKHKLKGTAWKMFESAATTGASLAILGLAGYTYHKYYKRLVLQKIDNAFNQGDPALNMATACYSDNEEPWVMLEAQDKIDSIVSGEEQGHYYLLIGEKGTGKTSMLLAAMHKIKGEACSMMEAHADPEIFRIRLGKALDFEFHEDYIGSLFSIRGPRDTTALLDIERALNKLEKVALIRRKATGKPLIMIVNSMHLLRNDDDGKDLLELLQQKAESWAASGLVTLIFNSDDYWVYERLKQYGSRMELISVKDLSKEKAITALKNYRAKYKHEMASMDVLERVYELVGGRLAFLNKVAREEDMIAKCKEICEIEKTWLLNKCGLLGSEMDDDVMDQQKFASSAMVLVKHLVDMEQADAGEYNRYNPEIGHELPEVPLYRARQIMTRAEFIEEFDHINIFTIDSKARVRADSVPMQNAFREVCGEPGFAQYLEDTLERISDIESLGRTKELTIKDLWHGGKYNMTVKNYRGNIEKSIHFDVTCGEKVKGEEEDDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8W9</accession>
    <name>D5G8W9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00004876001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430053">
        <property type="protein sequence ID" value="CAZ80962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836771.1">
        <property type="nucleotide sequence ID" value="XM_002836725.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8W9"/>
    <dbReference type="STRING" id="656061.D5G8W9"/>
    <dbReference type="GeneID" id="9181245"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004876001"/>
    <dbReference type="eggNOG" id="ENOG502S2D0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_361001_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8W9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd12432">
        <property type="entry name" value="RRM_ACINU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.30">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034257">
        <property type="entry name" value="Acinus_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032552">
        <property type="entry name" value="RSB_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003034">
        <property type="entry name" value="SAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036361">
        <property type="entry name" value="SAP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47031">
        <property type="entry name" value="SAP DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47031:SF3">
        <property type="entry name" value="SAP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16294">
        <property type="entry name" value="RSB_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02037">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00513">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68906">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50800">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAP" evidence="2">
        <location>
            <begin position="7"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="90"/>
        </location>
    </feature>
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            <end position="204"/>
        </location>
    </feature>
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            <begin position="228"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="346"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="654"/>
            <end position="677"/>
        </location>
    </feature>
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        <location>
            <begin position="754"/>
            <end position="775"/>
        </location>
    </feature>
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        <location>
            <begin position="80"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="356"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="469"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="760"/>
            <end position="769"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ80962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="775" mass="83749" checksum="836D2BC9881F278D" modified="2010-06-15" version="1">MLDPTTLSSLKVTELKAELKKRGLPVGGLKQVLVERLAEQVIQERAAAEAEAAALAEEERAAVDDAQGEEVVNGDGIAPDPQPEPQPEPIPEVVEGEKEVVPVPQAEPEAIVVEATQAVVVEGNVIEPEVIQEEAPKPEAPTEDEPAVIETASEPAPEPATESVAATEVVEGVVFEEKVVEPEVVERASGPEAEPAPEPVEADPVETELAETELVKTEPVETGLVETEPAETGLVETEPAETGLVETEPAETKVVERVEEEETGELVLESGAKFEDEVVEEAALKEVTTKTEIASDETPGTGPIIEKKEEIPVAGQPILEFEPEAEMERMKPEGLVIAAVEETTREMVKDEDSNMVDASSLSLSPEKTIVPELTPAHDDTTAESTLKPGPETELQQPSVDLMDTSEGQTDIRKRKRRSITPTPSSARVGEPENQQSNEGATSKKPRRDSTENQPQRTRPKDARFKGLFNDSSSTVTANNPPTASHHYNQAADQAMNQLITQIEDEDDAPIPPSIHPATRALYIRNFARPLSEPALKAHLTALASPPSSTSAVTTSQPPEPPIEYLYLDTIKSHALIIFTTLTAATRVRVGIHTKVWPNERSRKPLWADFVPEESVAGWVDREKQRGPGARWEVIYDREAGEGGVRAELEEVGGRRRIGPGGNTGVEVLGAPTGPRGERRGVQITEREFGARSNFSAATTDPTITNSATQRTATTGGGGRIVVKDLDQLFRSTVTKPKLYWIPVDPDISRERLAAYERGRGNTGNAGAGSGFRRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>D5G965</accession>
    <name>D5G965_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00003165001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430055">
        <property type="protein sequence ID" value="CAZ81058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836867.1">
        <property type="nucleotide sequence ID" value="XM_002836821.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G965"/>
    <dbReference type="STRING" id="656061.D5G965"/>
    <dbReference type="GeneID" id="9184344"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003165001"/>
    <dbReference type="eggNOG" id="KOG2257">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038387_0_0_1"/>
    <dbReference type="InParanoid" id="D5G965"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052263">
        <property type="entry name" value="GPI_Anchor_Biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013717">
        <property type="entry name" value="PIG-P"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46346">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46346:SF1">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08510">
        <property type="entry name" value="PIG-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="174"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="213"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="PIG-P" evidence="7">
        <location>
            <begin position="171"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="111"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="268"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="15"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="39"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="78"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="115"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="127"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="275"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ81058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="349" mass="36628" checksum="1AACFAF8EED7B8E5" modified="2010-06-15" version="1">MPRPTSASLHPPPITTSASSPNLTSHLNSDENRRTRNSFGGGNGGIGGIGAGGNGSVGGTGGGGGNSALTDSDDDDTMSSSSSSMPLPLSGGGFNGMGGIGGGMLHPSSFAPPFYNRPPTPLPPSPSLTSLLRPPFTPSRPTTPDSSDSEAVATSSRIAQSTPRAAPKVPTYEYYGFVLYLVSSLAFLIYIIWSFSPSPLLHAVGIYYYPGRWWALALPSFLVMLVVYIYVALAAYNTGYLTRGLDHLECIVDDAAKIANVDFGGGGEAPEDESAGNSGVSVSIGSSENDGQRDWKALWNRGTDAVMDIPVGGVCEVLYGDGREREQWEIKIDEQDWGSFEVATEVDVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9A8</accession>
    <name>D5G9A8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">Arabinan endo-1,5-alpha-L-arabinosidase</fullName>
            <ecNumber evidence="4 7">3.2.1.99</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00003229001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>Endohydrolysis of (1-&gt;5)-alpha-arabinofuranosidic linkages in (1-&gt;5)-arabinans.</text>
            <dbReference type="EC" id="3.2.1.99"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Glycan metabolism; L-arabinan degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the glycosyl hydrolase 43 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.99" evidence="4 7"/>
    <dbReference type="EMBL" id="FN430057">
        <property type="protein sequence ID" value="CAZ81101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836910.1">
        <property type="nucleotide sequence ID" value="XM_002836864.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9A8"/>
    <dbReference type="STRING" id="656061.D5G9A8"/>
    <dbReference type="GeneID" id="9182852"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003229001"/>
    <dbReference type="eggNOG" id="ENOG502QTQG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009397_5_0_1"/>
    <dbReference type="InParanoid" id="D5G9A8"/>
    <dbReference type="OMA" id="GHLWAPD"/>
    <dbReference type="UniPathway" id="UPA00667"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046558">
        <property type="term" value="F:arabinan endo-1,5-alpha-L-arabinosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031222">
        <property type="term" value="P:arabinan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd18831">
        <property type="entry name" value="GH43_AnAbnA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.115.10.20">
        <property type="entry name" value="Glycosyl hydrolase domain, family 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050727">
        <property type="entry name" value="GH43_arabinanases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006710">
        <property type="entry name" value="Glyco_hydro_43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016840">
        <property type="entry name" value="Glyco_hydro_43_endo_a_Ara-ase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023296">
        <property type="entry name" value="Glyco_hydro_beta-prop_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43301">
        <property type="entry name" value="ARABINAN ENDO-1,5-ALPHA-L-ARABINOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43301:SF3">
        <property type="entry name" value="ARABINAN ENDO-1,5-ALPHA-L-ARABINOSIDASE A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04616">
        <property type="entry name" value="Glyco_hydro_43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF026534">
        <property type="entry name" value="Endo_alpha-L-arabinosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75005">
        <property type="entry name" value="Arabinanase/levansucrase/invertase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072019" description="Arabinan endo-1,5-alpha-L-arabinosidase" evidence="10">
        <location>
            <begin position="20"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="34"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="201"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalytic activity, responsible for pKa modulation of the active site Glu and correct orientation of both the proton donor and substrate" evidence="9">
        <location>
            <position position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR026534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR606710-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR606710-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ81101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="323" mass="34744" checksum="4FE27EFDA55F5FBD" modified="2010-06-15" version="1">MMLLKLILLLVAFVSTAISYGNPGACSNACNVHDPAVIRRDSDGTYFRFSTGNKIQIATSSSLSGPWTIKGSALSMGSKINLAGNQDLWAPDVTKVGDNYYLYYSVSTFGSQESAIGVATSKTMDPGTWTDYGRTGVVSHAGSAYNAIDGNVVLAGDGSYYMSFGSFWGDIYNVPMNNPPLTASGSSTRIAYNSTGSHAIEGSFIWYRSPYYYLFFSAGTCCGYDSNRPAPGMEYRINVCRSTSVNREYVDKNGVSCTAKGGSTVLASHDNVYGPGGQGVFLDNVSGGEVLYYHYVDTTIGYGDGQKRFGWNLINWSTGWPVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9P1</accession>
    <name>D5G9P1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00005002001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430064">
        <property type="protein sequence ID" value="CAZ81234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837043.1">
        <property type="nucleotide sequence ID" value="XM_002836997.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9P1"/>
    <dbReference type="GeneID" id="9185371"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005002001"/>
    <dbReference type="HOGENOM" id="CLU_2759635_0_0_1"/>
    <dbReference type="InParanoid" id="D5G9P1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="70" mass="8189" checksum="1BBA274F0CF89555" modified="2010-06-15" version="1">MNRPQYYSNPSLALCPGNMAISLLLQHFPYGTYTPRSPALPYNIHPYYDSFPRERPETTAETRQGVKWRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9X4</accession>
    <name>D5G9X4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00003418001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430065">
        <property type="protein sequence ID" value="CAZ81317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837126.1">
        <property type="nucleotide sequence ID" value="XM_002837080.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9X4"/>
    <dbReference type="FunCoup" id="D5G9X4">
        <property type="interactions" value="194"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G9X4"/>
    <dbReference type="GeneID" id="9188559"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003418001"/>
    <dbReference type="eggNOG" id="KOG2645">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017594_4_0_1"/>
    <dbReference type="InParanoid" id="D5G9X4"/>
    <dbReference type="OMA" id="MMGGKRL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047429">
        <property type="term" value="F:nucleoside triphosphate diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017111">
        <property type="term" value="F:ribonucleoside triphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009141">
        <property type="term" value="P:nucleoside triphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16018">
        <property type="entry name" value="Enpp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.180:FF:000003">
        <property type="entry name" value="Type I phosphodiesterase/nucleotide pyrophosphatase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151:SF120">
        <property type="entry name" value="BIS(5'-ADENOSYL)-TRIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151">
        <property type="entry name" value="ECTONUCLEOTIDE PYROPHOSPHATASE/PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="637"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="649"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="674"/>
            <end position="684"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ81317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="715" mass="78766" checksum="576E2C9BF7B1794A" modified="2010-06-15" version="1">MTTAQYAAVPANAAHDILSGSSDDEGSCADHEVLREEEEREKLLAGRNGLFGSIGGKPGVKIGRKVRGVRGKKKGVRDVVQMMGGKRLGMEEGGEFGLGADSDDGANSEEGGEVDLLREKEVFDRKPRKPSVSRVLLYTVSLLTLLLLLISASLSLSHGHRPRTAPLGITKDTRFSNGTHDYMPTTLLISLDGFRADFLDRAMSPELTAMASSGLSPRWLTPAFPSVTFPNHWTIVTGLYPESHGIVGNSFWDPALEKEFFYTDPARSLQGEWWGGEPLWATAERQGVKSAVHMWPGSEANDGWGISYLDHFNSTESLKRKTARILEWLDLPLQDRPQFIAAYVPNVDVTGHKFGPNTTALNLVIREVDAMLTDLLDGLNARNLTDLINIIVVSDHGMASTSHERLIYIDEIIDMSLIEHTDGWPLYGLRPHANVNITALYETLLSETLMNQAKGKKHWDVYLRDENMPPRWHFSNNNRIAPLWVVPETGYAIVTREEYDVDAPSKGRYEPAGLHGYDNLHPLMRAIFVAKGPAFRHIYGKGIEGAVDVGVVVEEFGNWEVHRIVCESLGIGEALGGTNATIKDLAAFKAVNGWPVAEGEEEGEVGTSTIAQVGETIAPTLTATVPTGAGEGVVSIQTIPADPNPQPNPNTTQLPEPNQNEPQNHPVESPEEGEEKKSDEKNPEDMNPWEYAKWKAEKLRIALEKWWEGVWVGDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>D5GA96</accession>
    <name>D5GA96_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="17">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">GSTUM_00005194001</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430075">
        <property type="protein sequence ID" value="CAZ81450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837259.1">
        <property type="nucleotide sequence ID" value="XM_002837213.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5GA96">
        <property type="interactions" value="969"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GA96"/>
    <dbReference type="GeneID" id="9185732"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005194001"/>
    <dbReference type="eggNOG" id="KOG0386">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000315_15_3_1"/>
    <dbReference type="InParanoid" id="D5GA96"/>
    <dbReference type="OMA" id="YGPGHKL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016514">
        <property type="term" value="C:SWI/SNF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140658">
        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140015">
        <property type="term" value="F:histone H3K14ac reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140008">
        <property type="term" value="F:histone H4 reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031492">
        <property type="term" value="F:nucleosomal DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000182">
        <property type="term" value="F:rDNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061629">
        <property type="term" value="F:RNA polymerase II-specific DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035973">
        <property type="term" value="P:aggrephagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042148">
        <property type="term" value="P:DNA strand invasion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900189">
        <property type="term" value="P:positive regulation of cell adhesion involved in single-species biofilm formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000219">
        <property type="term" value="P:positive regulation of invasive growth in response to glucose limitation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031496">
        <property type="term" value="P:positive regulation of mating type switching"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045815">
        <property type="term" value="P:transcription initiation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17996">
        <property type="entry name" value="DEXHc_SMARCA2_SMARCA4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000008">
        <property type="entry name" value="Chromatin structure-remodeling complex subunit snf21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000843">
        <property type="entry name" value="Chromatin structure-remodeling complex subunit snf21"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014978">
        <property type="entry name" value="Gln-Leu-Gln_QLQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014012">
        <property type="entry name" value="HSA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029295">
        <property type="entry name" value="SnAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10799">
        <property type="entry name" value="SNF2/RAD54 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07529">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08880">
        <property type="entry name" value="QLQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14619">
        <property type="entry name" value="SnAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="QLQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01314">
        <property type="entry name" value="SnAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51204">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51666">
        <property type="entry name" value="QLQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 10" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="QLQ" evidence="16">
        <location>
            <begin position="86"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="HSA" evidence="15">
        <location>
            <begin position="311"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="490"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="802"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="12">
        <location>
            <begin position="1194"/>
            <end position="1264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="145"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="292"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="423"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1094"/>
            <end position="1171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1285"/>
            <end position="1362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="22"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="41"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="145"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="297"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="437"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1134"/>
            <end position="1149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1300"/>
            <end position="1324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1332"/>
            <end position="1349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1351"/>
            <end position="1362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAZ81450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1362" mass="155165" checksum="D3F272C2A4951CF8" modified="2010-06-15" version="1">MQQQQLHLQQQQQQQQRAQQLPVSQNGAAQLNGRSPAHPQHANNIASSLQQRPAADCPPSTNGVGVPQPSPVPSALPKNASSPNSTFTQDQLGILRNQIMAFKMISKNLAVPQLIRSAIFSSSVPKPDAVGSEVAAANAVVDPTAKEEVEAEKPEAPKKRRSGYETFTSPWASLQPKISYHDHGSRAKRLMIPSIMPSGIDVSLLQDERERVIFNRISARKAELENLPSNIGEVDTRKTSAVFTDSLKLKALIEYKALCLLPKQRALRQEMVNSMVHAENLAVTSNRAMFRRRKKQSLREARITEKLEKQQRDQREQRERRKHQEYLQGVIAHGQEIKAEARARDAKAQKLGRMMLQQHQHMEKEEQKRMERTAKQRLLALKANDEVAYLKLLDQAKDTRITHLLRQTDSFLESLAASVRQQQRDAVETYGKPEGYQEPESEDEEEDSTGEKIDYYAVAHRIQENVTEQPTILTGGKLKDYQLKGLQWMISLYNNNLNGILADEMGLGKTIQTISLITYLIERKKQNGPFLVIVPLSTLTNWNLEFEKWAPGVGKIVYKGPPAVRKNQQYDIKFSNWQVLLTTYEYIIKDRPLLSKVKWNYMIIDEGHRMKNSQSKLSATLTTYYNCRYRLILTGTPLQNNLPELWALLNFVLPTIFKSVKSFDEWFNTPFANTGGQDKMELTEEEALLVIRRLHKVLRPFLLRRLKKDVESELPDKVERVIKCKFSALQQKLYQQMMNNGILYVNEPDKGGKLGVRGLSNMIMQLRKLCNHPFVFEEVESAINPTKVNNDALWRTAGKFELLDRLLPKFFATRHRVLMFFQMTQIMNIMEDFLHLRGFRYLRLDGSTKADDRSALLKEFNAPDSPYFIFLLSTRAGGLGLNLQTADTVIIYDSDWNPHQDLQAQDRAHRIGQKNEVRILRLITSNSVEERILERAQYKLDIDGKVIQAGKFDNKSTNEERDALLRVMLEADEKEVGDSEELDDDELNEIISRNDNELTLFKQMDIDREKNSLYGKGKPLDRLYTEAELPEIYLHDDLIPIEEPTGPVGRGARERKVTNYDDGLTEEQWLDAIDNDDDTIEDAVRRKRDRIERRQVNKAKRAGELSESSPPPESPGVEDTPPAVQPKRKRGRKPAAEKRRIEEVEEPKIHPRKRQRTVKGYAPPDAVSPGNRAMLQRSCEAIFDAVHSLEDPETERVRSEIFEKLPSKKLFPDYYHIIGRPISFNEIKKRIRNGDYLDLEGFHADFKLMFKNARTYNEEGSKVYEDANAMDAEFDRRYAEEKKTLDALTNPEHSSVAGDGTSTGPISSAGTPAAVANSTGNNGTPRIRIKSRATAAVATTTTTVASRASPPEDDEGDSDDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAG0</accession>
    <name>D5GAG0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00005272001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430075">
        <property type="protein sequence ID" value="CAZ81503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837312.1">
        <property type="nucleotide sequence ID" value="XM_002837266.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAG0"/>
    <dbReference type="GeneID" id="9187443"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005272001"/>
    <dbReference type="HOGENOM" id="CLU_2016888_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAG0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ81503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="123" mass="13599" checksum="E3986DC87AE65B7D" modified="2010-06-15" version="1">MQGEKRQEMNDTHLRGTMQLTSNAGSYWGKSRRKVQICRTPEFGQPIPQTNLIDNVRSEAKIITLRVRGLGEFFFSEDFFPSARINLPAKEGQSSVMSKIFFPAGGSDIMPLGGITGAGMILC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAG1</accession>
    <name>D5GAG1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00005273001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430075">
        <property type="protein sequence ID" value="CAZ81504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837313.1">
        <property type="nucleotide sequence ID" value="XM_002837267.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAG1"/>
    <dbReference type="STRING" id="656061.D5GAG1"/>
    <dbReference type="GeneID" id="9187444"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005273001"/>
    <dbReference type="eggNOG" id="ENOG502RXR7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_624367_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAG1"/>
    <dbReference type="OMA" id="WITVPWS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42032">
        <property type="entry name" value="YALI0E30679P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42032:SF1">
        <property type="entry name" value="YALI0E30679P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="136"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="215"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="413"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="45"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="416"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ81504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="439" mass="47498" checksum="D5EB934AC105CBE2" modified="2010-06-15" version="1">MTSRFSASSAATAATAAAAQSPLVYRSTAEGGGVLGGGGGGGNGMRSPRNNMNGGASSAGTPRKWPAGSPPMNAPPESPLQSTSRRNSLLPENLQDATKVLVPLPTDADSSPWHNVPLAFALLPALGGILFTDGSYFFTDVILIFLVAVFLHWLVKFPWEWYGSSQALRYERDAFEALATPVEFLKDGDAPDATRSAHEERLRAAAARELRMNEVLALLACFVGPCVGGSLLHAIRSQLSRPSEGLVSNFNLTIFVLAAELRPAAQVVKLIRNRSLYLHSVVHHPPMTRIDALTARLEELSAEVRDLTLMTTKAVDRGSHLDALTRAVRRYEKRETVHSAQTENRILEIDNRLNDVLTLTAAASARQNHTTFSMILFEWACAAIVIPFSLTWKLISLPAKAVETLAGFGKNQKLLKSGRRQQPRLKGRSKMGKSKDAGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBB1</accession>
    <name>D5GBB1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00000398001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat MET30/SCONB/SCON-2 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430090">
        <property type="protein sequence ID" value="CAZ81804.1"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="209"/>
            <end position="255"/>
        </location>
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            <begin position="701"/>
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        <source>
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        </source>
    </evidence>
    <sequence length="717" mass="79506" checksum="AB4D9603A6787330" modified="2010-06-15" version="1">MTVLTPSSTAPPKLPLPRLPSAPTRTPTSLPAVPPPPLKNTCEDARAARPSKKRKISSIFNSNTCTEEMDESEPELFVPAIPADATENVKKGVGPFLSKHIPEQYAPQGPRGQQDTQQDESEKGNTRYCYRHRPDIKCRRRADEPTMEQLQKQMETLPAADQQAISHVWSLFSAAPAIHRNMMLQGILTQCCFPQLSFISAALSTLIRIDFLTALPPEIAFKILCYLDTASLCKAAQVSHRWRMLADDDVVWHKMCEQHIDRKCDKCGWGLPLLERKRLRATKRQMELRAKEPDLIFTAIVPPPSKTRPWKDVYSERYKVESNWRRGRCTKKIFQGHSDGIMCLQFDDNTLATGSYDRTIKIWDIESGQEIRTLVGHDLGVRALMFDDQKLISGSLDKSLKIWNWRTGECISTLRGHAAGVICVHFNENLLASGSVDKTIKIWNFADKSRFALKGHTDWVNSVRLDSGSRTVFSASDDQTVRMWDLDTKACIRVFEGHVGHVQQVIPLQLLNHQSPRGGAAATDGEDSSSSTFSPPTNPQIPPSRHHPNHPLEEYREANASDSPNRPRARAPPPKQMITAALDSQVKFWDVATGKCTKTLFGHVEGVWALAADSLRVISGAQDRMVKVWDVRTGRCQRTITGHSGPVTCVGLSDSKMVTGGEDGEARLYCFKSEGSSANGWTTPPEMVGSSDSGSAIAYSDGQAGDYYDGNGDGDSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBW1</accession>
    <name>D5GBW1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00005613001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ81961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002837724.1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ81961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="501" mass="59020" checksum="E1CC7005B69B9AC9" modified="2010-06-15" version="1">MPIIVDPLRQFFEQFDFERYTYDPDAPAHEEFDRLCEARQWGSSKIRRYRAEFLLALPEEQDPLSQFFEQFDFERYTYDPDIPAHEEFERLCEARQWGPSKIRRYKAEFLLALSQEQDPLSQFFEQFDFERYTYDPDIPPRKEFDRLCEARQWGSSKISHHETEFFLALSRELDRSGSSVGIEVIKFFRRYEYSLFTYDIDEPVQSEFQRLVGLRGWGNARLSKVTREFNDAVALDARGQSGYSTSEPTTSENPGTLEEDLLADWLRARECPGYEYSGDLHELEFKKLADAKRREWLRCLSLERGRPTSIEERRGWKISPEFLKLRNGFYAVVEKVFNLQLGNFCRVTGLTPWQVFVELYGNGQQNVGKRDAKTILHRVFINIFDFLDAFGETLKNPRTTNGKKALRKLKPLARELQFPSNLMLGVYSALTNRVFPIDVVDRQGTLVLLLQPIKVYLRSFDDVMREFEEEAVDELQEAEEEGRVGIRRLLLSREWDSLDSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBX0</accession>
    <name>D5GBX0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00005624001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ81970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837779.1">
        <property type="nucleotide sequence ID" value="XM_002837733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBX0"/>
    <dbReference type="STRING" id="656061.D5GBX0"/>
    <dbReference type="GeneID" id="9185405"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005624001"/>
    <dbReference type="HOGENOM" id="CLU_1171347_0_0_1"/>
    <dbReference type="InParanoid" id="D5GBX0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.50.70">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="98"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="182"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="237" mass="23488" checksum="72D87AB31B886EF3" modified="2010-06-15" version="1">MNCAAVRITGSGYSTLEDYDDMWVGDMNLPGRIKTGECRSKVGSALVYPNPGPFITIKEVPGIPFGPPTGGTCFVGPGKGKSTGSGSGNSGQGTGIVSSSTPSTTTRISKGKTTAGGAAATSIAGGGSGGAKTTDPANGPMFPTTEEEVVPPLRTLTFPMTGTKTKASAGYKSTSSSCAVGGSEGSGAGTGTGGSTTWGSGSGSGSGADNTTPATTQKPWTKIYTPTTFQRRFASVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GC33</accession>
    <name>D5GC33_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="15">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">GSTUM_00000544001</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430099">
        <property type="protein sequence ID" value="CAZ82076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837885.1">
        <property type="nucleotide sequence ID" value="XM_002837839.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GC33"/>
    <dbReference type="STRING" id="656061.D5GC33"/>
    <dbReference type="GeneID" id="9181345"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000544001"/>
    <dbReference type="eggNOG" id="KOG0158">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_14_11_1"/>
    <dbReference type="InParanoid" id="D5GC33"/>
    <dbReference type="OMA" id="WMPLDTW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902181">
        <property type="term" value="P:verruculogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11061">
        <property type="entry name" value="CYP67-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000063">
        <property type="entry name" value="Cytochrome P450 monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050121">
        <property type="entry name" value="Cytochrome_P450_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305:SF237">
        <property type="entry name" value="CYTOCHROME P450 MONOOXYGENASE ATNE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12 13" id="KW-0349">Heme</keyword>
    <keyword evidence="9 12 13" id="KW-0408">Iron</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 13" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="8 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="12">
        <location>
            <position position="442"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAZ82076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="508" mass="57420" checksum="21612FD52E2D3186" modified="2010-06-15" version="1">MTFLDLARLLFTSFAVYISSLVVYRLFFHPLARIPGPFLAKITDWYTVYHAYRGDRHLALYRSHEKYGPVIRFAPNLISFNSASSLKAIYGHTALSRSLQKSQFYSAFPAVKGVHNTHNAISKAEHGRKRRVLSAAFSDAALKSMEDLVLSNIDVFSAKVNDAFKHGVSVDIGDLFSWLTFDVMGELCFGRSFGMLTEETTRFVTNLISQAAHNHYINGNYLPLTTLKLSKILFPTISRDRWKFIEHSRAYADERMSLGRGYKKDFFYYLLDAKDPETGEGFETKELWGEANVLMIAGSDTTATAMSAAMFYLCRNPQTLQTLKNEIRSTFTDKEQIIGGKELNDCHYLKACIDEAMRLAPPVPGLLPREVIAKGGIEIDGLYIPEGTVVGTPIYTLHHNPAYFPDAFSFKPERWLSTCTSEEEAEAARSAFTPFSIGPRGCIGKSVAYMELRLTLARLLFEYDCEEVETEGKGALWKEGHAMVDGEYRLMDHFTSRKEGPVMRFSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>D5GC41</accession>
    <name>D5GC41_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00000553001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430100">
        <property type="protein sequence ID" value="CAZ82084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837893.1">
        <property type="nucleotide sequence ID" value="XM_002837847.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GC41"/>
    <dbReference type="GeneID" id="9181373"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000553001"/>
    <dbReference type="HOGENOM" id="CLU_405003_0_0_1"/>
    <dbReference type="InParanoid" id="D5GC41"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="14"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="679" mass="78168" checksum="F966136D15C17881" modified="2010-06-15" version="1">MGLQARSKRNSIPFDMIGNLPEDLVAEVLSYLPDMSDMRRLIHWQTVSKTWRRILHSPMTKVYHQLLLHNVHLRALLPPPHYYGGNHSFYSYATFRRALRTDLAIRKGNFRSATPWMYSACGGYVKEERYGAVDGGPFFSDGFLVWLERSSNLMTGGKTVFVRYLDFSNVTRTSAKQEPIAGSITVAQFERPGESEAQLETLFRNTSNHWVALGYGSGIVAFWSFMDKYITVRGVHESNKLRWKLPLSALLPPPDGGNLYVREIFCSKDYLVISISRSATLQVFSMSSRKRIHLINLRGVYHNKETTDFYYDTVASGNAEYAAGIGDNRYFMYANTEPTAFYHNIIPGYREPPPEFYFYCWDLKPQSPDTESRCSWDDQPESLEAEELLSNIQEPQFNIPIDPLASNQSISSADAPKPPKYNPPLTFLRPISRHPISLSWKAPVRFTSPKIKLNTSTRELLFYCPQHCHPPHIYYRFSYPDPPETLRIEEKPWCDHCVRNPYRHTARGYTPALREDDRQFAHLELKYNTELVFSDQSRRYRLALSPISVLRGGKSLRGAITGRKEHRCRLQMLRPNPPPPALEDRRRASVGGGIPGEVKAHWDMSMGKKEPWRSAQGYADRVWVGGGWEGVAVLFNGKFAVWRWGDYEAEEEEVFKEVAVTRRLLKRQSRRQSWLPGKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GC54</accession>
    <name>D5GC54_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 10">Peroxisomal membrane protein PEX14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 10">Peroxin-14</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00000569001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the PEX13-PEX14 docking complex, a translocon channel that specifically mediates the import of peroxisomal cargo proteins bound to PEX5 receptor. The PEX13-PEX14 docking complex forms a large import pore which can be opened to a diameter of about 9 nm. Mechanistically, PEX5 receptor along with cargo proteins associates with the PEX14 subunit of the PEX13-PEX14 docking complex in the cytosol, leading to the insertion of the receptor into the organelle membrane with the concomitant translocation of the cargo into the peroxisome matrix.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9 10">Peroxisome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the peroxin-14 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430100">
        <property type="protein sequence ID" value="CAZ82097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837906.1">
        <property type="nucleotide sequence ID" value="XM_002837860.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GC54"/>
    <dbReference type="FunCoup" id="D5GC54">
        <property type="interactions" value="58"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GC54"/>
    <dbReference type="GeneID" id="9181366"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000569001"/>
    <dbReference type="eggNOG" id="KOG2629">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045718_0_0_1"/>
    <dbReference type="InParanoid" id="D5GC54"/>
    <dbReference type="OMA" id="YNQWQPP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990429">
        <property type="term" value="C:peroxisomal importomer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016560">
        <property type="term" value="P:protein import into peroxisome matrix, docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025655">
        <property type="entry name" value="PEX14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006785">
        <property type="entry name" value="Pex14_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23058">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23058:SF0">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04695">
        <property type="entry name" value="Pex14_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="3 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0811">Translocation</keyword>
    <keyword evidence="2 10" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Peroxisome membrane anchor protein Pex14p N-terminal" evidence="12">
        <location>
            <begin position="2"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="246"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="319"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU367032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF04695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ82097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="343" mass="36004" checksum="D7EDE87F346801F2" modified="2010-06-15" version="1">MREDLISSAVILSDPAVAAAPLEKRIAFLQSKNLTKDEVDAALLRAGGTPGPVSTSLVPSGTYPPVASPPAPGYNTGYYAPQQYQHIAAGPRDRDWRDWFIMATVTTTIGFGLYHLAKRYVAPMISPPTPPQLETDKALIDAKFAEAFDLLDSLKNDTAALKTAEEERKNRVDAALAEVENVIQGLKEAGKRREDESKRVGDEVRGLRDLIPKALEGQKELQKKSLEDLQTELKSLKQLVMNRTGAARPSFLPNGPTGTPPLDPGSATGGAGQTLGSAVTQQVLGTGGNALGGLQRKEGSALPFGARSAIPAWQMAAAAKPSPAVPAVGASSSTGEGAEASSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>D5GC62</accession>
    <name>D5GC62_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00000600001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the bZIP family. YAP subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430103">
        <property type="protein sequence ID" value="CAZ82105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837914.1">
        <property type="nucleotide sequence ID" value="XM_002837868.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GC62"/>
    <dbReference type="SMR" id="D5GC62"/>
    <dbReference type="FunCoup" id="D5GC62">
        <property type="interactions" value="2688"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GC62"/>
    <dbReference type="GeneID" id="9184797"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000600001"/>
    <dbReference type="eggNOG" id="ENOG502RPD7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011807_0_0_1"/>
    <dbReference type="InParanoid" id="D5GC62"/>
    <dbReference type="OMA" id="LNMACGN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14688">
        <property type="entry name" value="bZIP_YAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000067">
        <property type="entry name" value="BZIP transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.100">
        <property type="entry name" value="YAP1 redox domain. Chain B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050936">
        <property type="entry name" value="AP-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013910">
        <property type="entry name" value="TF_PAP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023167">
        <property type="entry name" value="Yap1_redox_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40621:SF6">
        <property type="entry name" value="AP-1-LIKE TRANSCRIPTION FACTOR YAP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40621">
        <property type="entry name" value="TRANSCRIPTION FACTOR KAPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08601">
        <property type="entry name" value="PAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111430">
        <property type="entry name" value="YAP1 redox domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="6">
        <location>
            <begin position="182"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="29"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="118"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="330"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="428"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="37"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="49"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="137"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="158"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="198"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="330"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="347"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="357"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="367"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="435"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ82105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="615" mass="65918" checksum="6FCF96537C6EA625" modified="2010-06-15" version="1">MTTAAPDSLYLSPDQQDLLLAALTSNSSSSPMFNTPSLSHHLSQSISSNRRSLPPQSHDTPDSVGSLYGNGTPPPTAALTGSFSDGTPLMGELDYQDWGEDLDVDNWDYDLGLPAGDADNNMDSHAGTPASASNSGGERDGEKRKNPPEAEDGSPSADPHDAEPKRRDDKTAKKPGRKPLTSEPTSKRKAQNRAAQRAFRERKEKHLKDLEQKVADLEKASESANHENSRLRGQVERLQIELKEYRRRLHTVGQSGSPLLGGASKFLSKNSVGTGGFQFEFPMFGNGLFIRNSENNSGLKSGALLDRLNAAGGTLAASEELKKSITNTNNVFNQNQNNDLSSSLQNTPRLNGGQNYASSPSASSVSQHGPGSSTGTSPEPTNGTVEGTITTSHNGDKYICKSGSLDGETETTFCEKLSMACGNPHNPIPKAAQLNSSSSSSSATTTTNTNNNTSSNTSLGWLTQQNGGNFDPVLFSDYRDPVNDINSGINMSFFDDAFAIPHTFNTDLGSPLNLAQTPKKLDLLAEIDQINDADLDEDEEEVVPADDPKQMLSCNKIWDRISNHPRFASGELDMDGLCSELRSKAKCSETGVVVAETDVQEVLTKVGVAHKDILG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCS7</accession>
    <name>D5GCS7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00006000001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430120">
        <property type="protein sequence ID" value="CAZ82320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838129.1">
        <property type="nucleotide sequence ID" value="XM_002838083.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCS7"/>
    <dbReference type="GeneID" id="9187939"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006000001"/>
    <dbReference type="HOGENOM" id="CLU_689273_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCS7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38846">
        <property type="entry name" value="C3H1-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38846:SF1">
        <property type="entry name" value="C3H1-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="400" mass="46401" checksum="EA266999623DDC9B" modified="2010-06-15" version="1">MTTQLSTAAIAIFFKSYNSPYYTYDPTLAVDDEFLRLQHEQQWGPEEVQEHRRLLYRAVQLAHFFWKHEWGGYAFREDAEYDHEFWRLCEKKRWDEGRISRAVDEFLKLSGGSVESLTAAGHTMPEETAPEEEAEAIPEQEPETTFTEQEDAETVHPEDPEAPGVQSAIAVFFVGHSCPGYTYAGRSPRTEFRNLIGARRRAWRAHDPDGTFNQDFQETEEFRTLDRDYHEAVEERFDGLLGMRGIVAGGYAMRPWESILELFRLGDIPISKNKASKLIKRIYVNIYDFLEFLEESNDSSTAEWHITGDEEYERACLLRHRSRGELAQYSRNNGKVYPLKMAKEDGTLALFLQPLWERAVLGWALQFGEGLDCQKGERVRGQRMLGTQEQDSHHTYQDSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCU6</accession>
    <name>D5GCU6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00000753001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430123">
        <property type="protein sequence ID" value="CAZ82339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838148.1">
        <property type="nucleotide sequence ID" value="XM_002838102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCU6"/>
    <dbReference type="GeneID" id="9182444"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000753001"/>
    <dbReference type="HOGENOM" id="CLU_2499508_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCU6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="86" mass="9422" checksum="546D2187F5E54A54" modified="2010-06-15" version="1">MILTILRPLPPSSPSLLSSSISPLPEDPKVLLRPSVIQTFLSFTEMTQCTAPLPSRADPGKNKRRFYHSCGSPGERITVEDGREGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCY1</accession>
    <name>D5GCY1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00000864001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430129">
        <property type="protein sequence ID" value="CAZ82374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838183.1">
        <property type="nucleotide sequence ID" value="XM_002838137.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCY1"/>
    <dbReference type="STRING" id="656061.D5GCY1"/>
    <dbReference type="GeneID" id="9183844"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000864001"/>
    <dbReference type="eggNOG" id="ENOG502ST8T">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029950_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCY1"/>
    <dbReference type="OMA" id="FAYRSIC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056929">
        <property type="entry name" value="Znf_RING-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25080">
        <property type="entry name" value="zf_RING-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RING zinc finger-like" evidence="2">
        <location>
            <begin position="354"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="179"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="295"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="258"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="319"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ82374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="413" mass="45387" checksum="47E42374BCF2A23A" modified="2010-06-15" version="1">MPAMAKSPLNSKNPTTVAKKIYAGAGIVGNGGHGEVVCPLFNADGSICRKKCVGNFAYRSICEHIRRAHPDNWIPKLPASPETFAKMVNLPPRGLAASAVNAQFHMRNPQHIPATPAMPVFRKAGKALPKHEDEQYVDGMSPPSPANESETLPSISLSTNPVTFHSNFPAVISDYGEHDCEDNHDFMGEDDLEEEMRSRRSDIDTEDDSDDGRRNPHRHQLLHHNHHRNHLQSQLPPLRTGKHPTSLKRKRQPYAASSPETGTIADNSSTQSSNASRWDELIEAATTRAVAIDPKPFHHHRPSHHHEYSHAPQTPLSPPATASSSSHHSLAPTASPPSTSASEFPMSGPRRLLCATCDNLTPIHEAFACTECMSGFCDECAFNSGRRGSCLRRMQSCWREIQAPQNCCPIKAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDB1</accession>
    <name>D5GDB1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00006131001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the KNR4/SMI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430142">
        <property type="protein sequence ID" value="CAZ82504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838313.1">
        <property type="nucleotide sequence ID" value="XM_002838267.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDB1"/>
    <dbReference type="STRING" id="656061.D5GDB1"/>
    <dbReference type="GeneID" id="9183968"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006131001"/>
    <dbReference type="eggNOG" id="ENOG502QTAZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024700_0_0_1"/>
    <dbReference type="InParanoid" id="D5GDB1"/>
    <dbReference type="OMA" id="ESLMVHD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097708">
        <property type="term" value="C:intracellular vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070880">
        <property type="term" value="P:fungal-type cell wall beta-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1580.10">
        <property type="entry name" value="SMI1/KNR4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009203">
        <property type="entry name" value="Knr4/Smi1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018958">
        <property type="entry name" value="Knr4/Smi1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037883">
        <property type="entry name" value="Knr4/Smi1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051873">
        <property type="entry name" value="KNR4/SMI1_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47432">
        <property type="entry name" value="CELL WALL ASSEMBLY REGULATOR SMI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47432:SF1">
        <property type="entry name" value="CELL WALL ASSEMBLY REGULATOR SMI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09346">
        <property type="entry name" value="SMI1_KNR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017023">
        <property type="entry name" value="KNR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00860">
        <property type="entry name" value="SMI1_KNR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160631">
        <property type="entry name" value="SMI1/KNR4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Knr4/Smi1-like" evidence="3">
        <location>
            <begin position="141"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="209"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="352"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="478"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="32"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="55"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="73"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="362"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ82504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="497" mass="54485" checksum="58FF4523AE956E9E" modified="2010-06-15" version="1">MTSNDRHANHDSPYRSHSNMGSQHIGVGRNPGLQSVSSPGADTSLNLGYRDENHSTTALASQNGVYSPRPLNGNIQGPNSPYSPGMRSSALQSDGSPGGIALQDFGTDGLPPPPPVSHSWSRIDRWCEDNYPELFDQLCTPASVNDINELEYNLDCSLPIEVRDSLQIHDGQERGGMPTGLIFGAMLLDCEEMLDEWKNWRKVEKEYLANPTLTPPTPNGEAGPSRLEAGSIGRQNLQARQDSQPEGAVVKAYAHPGWIPMVRDWGGNNIAVDLAPGPNGTWGQVILFGRDYDTKFVVAKSWPHFLAMVADDLASPHWWVDEETQELKLKDPRAPRSEPPYMEILRVRNERKYGRRRVRKSMSPTQSPHPSTMSPTAFGVVNGQGPRGGLTRPSREESTMSDNHLKPPSKKLSKPLRSVTEELPSPIPVHTKAQNSSSKGPAANLLDEPLLEEKPSDTIQRLEVVALDDSGKLKRAATDIVSHPPPSVAVQRSSLEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDN3</accession>
    <name>D5GDN3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00006197001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430153">
        <property type="protein sequence ID" value="CAZ82626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838435.1">
        <property type="nucleotide sequence ID" value="XM_002838389.1"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GDN3"/>
    <dbReference type="GeneID" id="9181631"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006197001"/>
    <dbReference type="eggNOG" id="KOG4159">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_568819_0_0_1"/>
    <dbReference type="InParanoid" id="D5GDN3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03105">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="460"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="SPX" evidence="2">
        <location>
            <begin position="1"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ82626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="480" mass="54396" checksum="F9DB023CB95C1735" modified="2010-06-15" version="1">MKFAHSFSQVLQEEDFPADWQAAAIRYRQLKKCIKKVQLELLDLGLTVEVLKGLAEERESPSFEYMFDGTVHSFQPKLVLTIDAVDLIPLDLNLSEDTRRSLECLVKKKLGREEVETKDAAVEGSTEMCKSIRTIEITLCSDSEFFHLLTSEITSLEALQSRSGSEVRSHILQLGQSVTAVAAPKSRNNKSDLYPWREIFRIYLETGIFFSNLETESHQERTIEEATERLTFFAGEIRRLGIQAKFKNPYSPSLFKMFLSVNMDVLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRGGKRTPAQGAEGNVCLVPIREIGIGGCIISGRSISPRRSRSSKPRMSTKSRWSIGRIFMANVLFREYGSFLFIYIPFSLFWRLAGVLFLAFSFFSFFSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDT3</accession>
    <name>D5GDT3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00006261001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430153">
        <property type="protein sequence ID" value="CAZ82676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838485.1">
        <property type="nucleotide sequence ID" value="XM_002838439.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDT3"/>
    <dbReference type="STRING" id="656061.D5GDT3"/>
    <dbReference type="GeneID" id="9184471"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006261001"/>
    <dbReference type="eggNOG" id="KOG2758">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031132_1_1_1"/>
    <dbReference type="InParanoid" id="D5GDT3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016650">
        <property type="entry name" value="eIF3e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019010">
        <property type="entry name" value="eIF3e_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10317">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09440">
        <property type="entry name" value="eIF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01186">
        <property type="entry name" value="eIF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Eukaryotic translation initiation factor 3 subunit E N-terminal" evidence="4">
        <location>
            <begin position="17"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ82676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="190" mass="21819" checksum="E327600C4CDB8020" modified="2010-06-15" version="1">MAENNAEGANDMAVSYDLIPKLLPHLDRHMIFPLLGFMGEQGRLTDQELAQARYDLLKNSNMTDFICDLYKEIHGTDEVPEELNKRQEVVEKLQGLGERSELLLNLLGNTEVLSNLRADKLQNLQYLQEKHDVTVEMVNVLYDYGQHQYSCGNYGEASDILYRFRVLSTDNEKTTQATWGKLAWFHGGDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5GE55</accession>
    <name>D5GE55_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00006385001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430164">
        <property type="protein sequence ID" value="CAZ82798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838607.1">
        <property type="nucleotide sequence ID" value="XM_002838561.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GE55"/>
    <dbReference type="FunCoup" id="D5GE55">
        <property type="interactions" value="25"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GE55"/>
    <dbReference type="GeneID" id="9183130"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006385001"/>
    <dbReference type="eggNOG" id="ENOG502QV5S">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044030_1_0_1"/>
    <dbReference type="InParanoid" id="D5GE55"/>
    <dbReference type="OMA" id="WEVAVWD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043007">
        <property type="term" value="P:maintenance of rDNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007096">
        <property type="term" value="P:regulation of exit from mitosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018819">
        <property type="entry name" value="Nur1/Mug154"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28293">
        <property type="entry name" value="NUCLEAR RIM PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28293:SF1">
        <property type="entry name" value="NUCLEAR RIM PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10332">
        <property type="entry name" value="DUF2418"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="237"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="342"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="376"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="395"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="437"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="464"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ82798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="484" mass="53045" checksum="4DFA289DDEEC07FF" modified="2010-06-15" version="1">MPPQRFVRRRPLAERLSSLVNPFDLFLALSIWLESHDWDNLQNTVKNPLGLALNLLCLISRANSNGGHRRSGAEDVLRRPAGGYSGGISSGGGAGLSYFFWILSWVLAAGSVFNAIYCFSRKKRYRLFESNIEVEPTTPSARRVPVDSSPTTTSPLRLFGNIMESFTGTPESRSHPDETRDVWEVSVWDPTPLSLRLFAIFSPVHVLIYFLVLPITSNATLSSSGFVKFPTPPTTNTVGTILMMLATQFILSVQLIHTQMSFAQQAKDTTLIHKEVLHEYDQKFVHPRLNVLKRDVAVQTANRESGEAASVSFFTPNLNRQGFRTAPNPNYADLTMRDKVGSMRHTPSTSARSPFSPMPLKGSMRQPQFGPVRRPASTSMIDSGSFGTDVDEDYGGSSDSAAMASTTTGRAEGRYGPGTPRMPKSSNVGMQGLVNMSMENSRNLSPSKLNSPLRRGYAARGSSYAGSLGTSLGSSVRRSANNDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>D5GE61</accession>
    <name>D5GE61_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00006395001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">tRNA nucleus export receptor which facilitates tRNA translocation across the nuclear pore complex. Involved in pre-tRNA splicing, probably by affecting the interaction of pre-tRNA with splicing endonuclease.</text>
    </comment>
    <dbReference type="EMBL" id="FN430164">
        <property type="protein sequence ID" value="CAZ82804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838613.1">
        <property type="nucleotide sequence ID" value="XM_002838567.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GE61"/>
    <dbReference type="FunCoup" id="D5GE61">
        <property type="interactions" value="1176"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GE61"/>
    <dbReference type="GeneID" id="9183136"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006395001"/>
    <dbReference type="eggNOG" id="KOG2081">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005996_0_0_1"/>
    <dbReference type="InParanoid" id="D5GE61"/>
    <dbReference type="OMA" id="LECITSW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000266">
        <property type="entry name" value="mRNA transport regulator MTR10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013598">
        <property type="entry name" value="Exportin-1/Importin-b-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051345">
        <property type="entry name" value="Importin_beta-like_NTR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057941">
        <property type="entry name" value="TPR_TNPO3_IPO13_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057942">
        <property type="entry name" value="TPR_TNPO3_IPO13_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058537">
        <property type="entry name" value="TPR_TNPO3_IPO13_4th"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12363:SF53">
        <property type="entry name" value="MRNA TRANSPORT REGULATOR MTR10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12363">
        <property type="entry name" value="TRANSPORTIN 3 AND IMPORTIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24138">
        <property type="entry name" value="TPR_TNPO3_IPO13_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24140">
        <property type="entry name" value="TPR_TNPO3_IPO13_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24139">
        <property type="entry name" value="TPR_TNPO3_IPO13_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08389">
        <property type="entry name" value="Xpo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="3">
        <location>
            <begin position="33"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00025147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ82804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="968" mass="108965" checksum="3925EBD7BE8A27F6" modified="2010-06-15" version="1">MASPNGPSDQFAPVLAALATMQGNVDREQKYQATQFLEHFQKSPEAWTVVHAILQSENAGVEAQLFAATTLKGKITYDIHQLPRDALVDLRNSLLSLLVAHRNGSRPIRTQLCVCLASLALQLLEWKDVIGLVVSTLGNDVESSVCLLEFLKILPEEVTEGRKVSLTEEELETRSRELLTDNATEVLRLLVQYAQSTAGSPPNPALISCVNSWLREIPVLDVIGTQLIDVIISALSSNAALDPAVECLCNIFRETREVDASQEAIRILYPRVMNLRPKIREAAEAEDPEKFRGYTRIFAEAGEAWVVLIARMPMDFRELVSAIAECAALDAERDVISLTFNFWYELKNYLVLEKYIEARVKIADIYSQLVDIMIRHLHYPEGSEADPFDGDRETEEKFREFRHSMGDVLKDCCEVIGSAACLGKAYSEIQTWMQRYATTAQKPVNGQVEHWQSLEAPLFSLRAMGRMIPADEEQVLPQIMTSLVQLPEHDKVRFAATLVLGRYTEWTSKHPAYLEPQLNYITRGFEHNSKDVARAAAMALKFFCQDCGKLLVDHVGQLHNFYEQVAGDLPIASLYEVTDGVAHVVAAQPLDKIYDALRLFCEPIAKRLMEKANSANDDKAKCELADYIQLLTIFVQVVHPHVPRGTLNPMIRFWSGLIPVLGTILDNFVEFSPVCERVSRCYRTMLVSYRTDMLPLLPQLAEKLVACFQKSHQGCFLWVTGAVIREFADEELVDEATRASVYQFLEQQCLNMFRLLNTKAATEIPDVIEDFFRLLVDGVMFHPYKLILSNLLQPIFEASLHSLSLEQVDPLVAVLQFLRDVLAYGRSSPPTSAYPDNPKEVQEAVKAMALSKGELITQKILSGLMYSFPRDCVPDSSGVLLALVELCPEPWLQWMKHTLELLPAGSISPAEAQKFLTTLEGAVSTRDFKKIRYSLQDFTNWYRRKNVTPRTAITGIEGIEGSRFSFSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5GE97</accession>
    <name>D5GE97_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Spindle pole body component</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00001234001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
            <location evidence="5">Cytoskeleton</location>
            <location evidence="5">Microtubule organizing center</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the TUBGCP family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430168">
        <property type="protein sequence ID" value="CAZ82840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838649.1">
        <property type="nucleotide sequence ID" value="XM_002838603.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GE97"/>
    <dbReference type="FunCoup" id="D5GE97">
        <property type="interactions" value="648"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GE97"/>
    <dbReference type="GeneID" id="9185495"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001234001"/>
    <dbReference type="eggNOG" id="KOG2001">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007738_0_1_1"/>
    <dbReference type="InParanoid" id="D5GE97"/>
    <dbReference type="OMA" id="QNMSGDP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000930">
        <property type="term" value="C:gamma-tubulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043015">
        <property type="term" value="F:gamma-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051011">
        <property type="term" value="F:microtubule minus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1900:FF:000011">
        <property type="entry name" value="Spindle pole body component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1900">
        <property type="entry name" value="Gamma-tubulin complex, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007259">
        <property type="entry name" value="GCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040457">
        <property type="entry name" value="GCP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042241">
        <property type="entry name" value="GCP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041470">
        <property type="entry name" value="GCP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302">
        <property type="entry name" value="GAMMA TUBULIN COMPLEX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302:SF13">
        <property type="entry name" value="GAMMA-TUBULIN COMPLEX COMPONENT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04130">
        <property type="entry name" value="GCP_C_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17681">
        <property type="entry name" value="GCP_N_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3 5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma tubulin complex component protein N-terminal" evidence="8">
        <location>
            <begin position="11"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="Gamma tubulin complex component C-terminal" evidence="7">
        <location>
            <begin position="352"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="150"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ82840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="743" mass="84266" checksum="754FF417D19A23D5" modified="2010-06-15" version="1">MPLSLQEASILEDLLFVFMGYEGQYIRFRSDYNPASESDRLAGPQFKILPGLDPSLRDLANTMLKMATHYGAVEAFAEVHSREEFGAVNHALCASIRKLLKEYLVLIAQLEHQFLTSPSFTLHVLHLRTLPTSHMMFQLYSLAREMLRRNSMLEDDLEDSVDDLDDMENILESLREGGDIAGSGGVPGSRICKGGSVLGLLTLRLASMAGDPAARSLLSLLLREASGPYMNMLNEWLHHGMIRDPHAEFLIKEQKSIRRERLEEDYTDEYWEKRYTIRDGDVPPQLEGVKEKVLLAGKYLNVVRECGGVDVSKDVGDVPKSFEDIRFLDNVNSAYAHANESLLNLLLTTHALPARLRSLKHYFFLDRSDFFSYFLELGATELQKQAKKVNVGKLQSLLDLALRQPGSIAASDPFKEDVKVQMNEVGLTKWLMRVVSVSGIDEGATTFEESHRTPAISSIDDDKSVIGFNALELDYSVPFPLSLVISRKTVLRYQLLFRYLLSLRHLETLLVQAWQDHNTSYTWKHKSKYPQVELWKRRAWTLRARMLVFVQQLLYFCTSEVVEPNWNGLMKRLGEVRTVDELMQDHVDFLDTCLKECMLTNSKLLKIHAKIMMTCTMFASYTSHLSKALITADPDLASPSAKYLGDEHAAFDQVRLDKLSDNLSKYEENFSRHLKILLDALNYYAATETVVLLGLCARLSTASNDAETHGRRILSYEEGILTNPVVPPTPRCANPELSKLLQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEN8</accession>
    <name>D5GEN8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00001294001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430181">
        <property type="protein sequence ID" value="CAZ82981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838790.1">
        <property type="nucleotide sequence ID" value="XM_002838744.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEN8"/>
    <dbReference type="STRING" id="656061.D5GEN8"/>
    <dbReference type="GeneID" id="9184901"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001294001"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019787_0_0_1"/>
    <dbReference type="InParanoid" id="D5GEN8"/>
    <dbReference type="OMA" id="FNAKAMY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000190">
        <property type="entry name" value="C2H2 finger domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051059">
        <property type="entry name" value="VerF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40626:SF8">
        <property type="entry name" value="C2H2 FINGER DOMAIN TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40626">
        <property type="entry name" value="MIP31509P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="26"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="54"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="80"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="155"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="182"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="792"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="183"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="204"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="223"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="795"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="807"/>
            <end position="816"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ82981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="816" mass="91150" checksum="487F23F90D4DB5D4" modified="2010-06-15" version="1">MHEVMTVPTPATQSTKKAVSVQERKYKCAYCSRAFSRSEHRSRHERSHTKERPFKCLKCRSTFVRRDLLLRHDRTVHAKDGGVPLHSEVKRRSTKNSASAGPTKPSIAIEQATIEQLEGDGGMDDLETAAALMTELHHAAAAAIVDHEQEMLLQEKSRRSMSSNPESVFDPNVSYASGAIPVQNMSTWDPPTPSSTAGPEPPRSHYMSHSQSSQDSFVSWGSNSLQIPSSASSVMERSLSADPGHSATVTPTSYASPHPLFTGPPSPANHSGPSASQAAPPPAPPSVDSDHERNMVLDNIREIDVERAVLDKFRLPSRSGLNRYLAAYFNLFHHHLPFLHTGSFSPSTLAPPLLLAVLSIGALYTFEKEQAYTLHMSSKVLVNNFIARHHEFNSRNCPLWTTQTLLLNMVFASWSGDPKGLDWACSMRDLLANIVAGGRYELQVRMDQRGDEMPTREEWIFEEGSKRTYYAIYIFFGLLTLTYNHIPAITYHEFDTLSLPCSETLWNLDGSDDQIWREHLSNASMPTFNEAHTRLINGDIPQYSVFAARVIISALFLDVLGLKRDTEIVDSVVSEYREKLSVALETWHRALEYCTPETMIVSLTAPQKGHPLVFNSMAIYRCAMARLEVDLVSIQETLRYHIPEDVASVMTSASGLIPRSEPMTKVIQQCFECFQIPALMGIRWVARTFALNWSVEHPLCGFELMLILSLWLYRLEEEMETDPPTEEEEAMLTKVRNLFDDDSVELYGSRLSAAVAMVWGGMIDEVVVWGITKLLGESFKLHSHTLALSDDIGMTSSSSPSPSATPEAEDLDSEME</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5GES2</accession>
    <name>D5GES2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00006580001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430186">
        <property type="protein sequence ID" value="CAZ83015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838824.1">
        <property type="nucleotide sequence ID" value="XM_002838778.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GES2"/>
    <dbReference type="GeneID" id="9184926"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006580001"/>
    <dbReference type="HOGENOM" id="CLU_2279494_0_0_1"/>
    <dbReference type="InParanoid" id="D5GES2"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ83015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="102" mass="11477" checksum="B7DBC155413DC2D9" modified="2010-06-15" version="1">MPYGTEPVREQKLQESLLTLFRAEALARGIEEPIGHEQVKSWLRFVVPEIEGAFGVPVYTAQEEMLARALDEIRRIFSQLGESPDKVLGYVFTGKRGGSRAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5GF68</accession>
    <name>D5GF68_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="11">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00006748001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the metaxin family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430208">
        <property type="protein sequence ID" value="CAZ83161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838970.1">
        <property type="nucleotide sequence ID" value="XM_002838924.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GF68"/>
    <dbReference type="STRING" id="656061.D5GF68"/>
    <dbReference type="GeneID" id="9188022"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006748001"/>
    <dbReference type="eggNOG" id="KOG3028">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032751_0_0_1"/>
    <dbReference type="InParanoid" id="D5GF68"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001401">
        <property type="term" value="C:SAM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03078">
        <property type="entry name" value="GST_N_Metaxin1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033468">
        <property type="entry name" value="Metaxin_GST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050931">
        <property type="entry name" value="Mito_Protein_Transport_Metaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019564">
        <property type="entry name" value="Sam37/metaxin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12289:SF41">
        <property type="entry name" value="FAILED AXON CONNECTIONS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12289">
        <property type="entry name" value="METAXIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17171">
        <property type="entry name" value="GST_C_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10568">
        <property type="entry name" value="Tom37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Mitochondrial outer membrane transport complex Sam37/metaxin N-terminal" evidence="9">
        <location>
            <begin position="20"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Metaxin glutathione S-transferase" evidence="10">
        <location>
            <begin position="203"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="329"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF10568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ83161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="472" mass="50496" checksum="388B7FBAA6F9EB95" modified="2010-06-15" version="1">MLELHIWGPSFTLPSISPQSLSTIAYLNLTQPSTSWTLIPSSNPLLSPSRELPALRDGATWASGFRGVVEHLRRKNGSSLDDKLAGAKRAEALAFGTYIESEGGKLLDLSMYLTDENFDVLRGVLAKLLPVPARYYFPGVLRREARRRCDGLISASSSASAVDAATPLPQASAMTAMALPAAKEKISAKAVAEKIKQVAIAANFLRTMQSLLGGRTYFFGDKPSSLDCLAAGYLSLALYAELPNGWLREEMLARHHGLCKYVDGVRGQMLGDGVDVAAVISGQAAMATPSGKIAGGGLPWGVVERQDVPWVAGFFLNRAMEIVGITPTERYGGGENEEERTEEKEGRRKGTKSLQRWATAKNVAVIAGCISTLIGYCVWSGLITMEFSGRAKDGAVGGVGGNEGEDNDDDGKDAENEMELLEGFGSAEAILGLGGRGTFSPKEEDEIDEVIDAEFCRGVNEEEGRGGIRPEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFA4</accession>
    <name>D5GFA4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Prohibitin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00006799001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Prohibitin probably acts as a holdase/unfoldase for the stabilization of newly synthesized mitochondrial proteins. Involved in mitophagy. Required for the switch to necrotrophic growth.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">The mitochondrial prohibitin complex consists of two subunits (PHB1 and PHB2). The subunits assemble into a membrane-associated ring-shaped supercomplex of approximately 1 mDa. Interacts with ATG24/SNX4; the interaction is direct and plays a role in mitophagy.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the prohibitin family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430208">
        <property type="protein sequence ID" value="CAZ83197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839006.1">
        <property type="nucleotide sequence ID" value="XM_002838960.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFA4"/>
    <dbReference type="FunCoup" id="D5GFA4">
        <property type="interactions" value="846"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GFA4"/>
    <dbReference type="GeneID" id="9188053"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006799001"/>
    <dbReference type="eggNOG" id="KOG3090">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047969_0_2_1"/>
    <dbReference type="InParanoid" id="D5GFA4"/>
    <dbReference type="OMA" id="NEGTHFQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035632">
        <property type="term" value="C:mitochondrial prohibitin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03401">
        <property type="entry name" value="SPFH_prohibitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.479.30:FF:000001">
        <property type="entry name" value="Prohibitin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="entry name" value="Band 7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000163">
        <property type="entry name" value="Prohibitin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23222">
        <property type="entry name" value="PROHIBITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23222:SF1">
        <property type="entry name" value="PROHIBITIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00679">
        <property type="entry name" value="PROHIBITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="Band 7/SPFH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="Band 7" evidence="9">
        <location>
            <begin position="54"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00053189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00066275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ83197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="302" mass="33308" checksum="7F05CA16C725B9E9" modified="2010-06-15" version="1">MDSNNPRETWNRIQAAITRVQRQGGPGRGPPKGVLGGGAALVLLGAAAVTFNSAIFNVDGGHRAIKYTRLGGVKKEIYNEGTHFVIPWFETPITYDVRAKPRNVASLTGTKDLQMVNITCRVLSRPHVDALPTIYRTLGVDYDERVLPSIVNEVLKSVVAQFNASQLITQRESVSRLVRDNLVKRAARFNIMLDDVSLTHLAFSPEFTAAVEAKQVAQQEAQRAAFIVDKARQEKQAMIVRAQGEARSAELIGDAIKKSKSYVELRKIENARNIATILQESGGRNKLYLDTDGLGLNVMEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFX8</accession>
    <name>D5GFX8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00001902001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.</text>
            <dbReference type="EC" id="3.2.1.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>alpha-D-galactosyl-(1-&gt;3)-1D-myo-inositol + sucrose = raffinose + myo-inositol</text>
            <dbReference type="Rhea" id="RHEA:20161"/>
            <dbReference type="ChEBI" id="CHEBI:16634"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
            <dbReference type="ChEBI" id="CHEBI:17505"/>
            <dbReference type="ChEBI" id="CHEBI:17992"/>
            <dbReference type="EC" id="2.4.1.82"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolases 36 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430224">
        <property type="protein sequence ID" value="CAZ83421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839230.1">
        <property type="nucleotide sequence ID" value="XM_002839184.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFX8"/>
    <dbReference type="STRING" id="656061.D5GFX8"/>
    <dbReference type="GeneID" id="9181766"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001902001"/>
    <dbReference type="eggNOG" id="ENOG502QPVE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006630_0_0_1"/>
    <dbReference type="InParanoid" id="D5GFX8"/>
    <dbReference type="OMA" id="HIFANAH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004557">
        <property type="term" value="F:alpha-galactosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047274">
        <property type="term" value="F:galactinol-sucrose galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008811">
        <property type="entry name" value="Glycosyl_hydrolases_36"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31268">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31268:SF32">
        <property type="entry name" value="GALACTINOL--SUCROSE GALACTOSYLTRANSFERASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05691">
        <property type="entry name" value="Raffinose_syn"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00049426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ83421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="918" mass="101445" checksum="9B8B7FCC3728AEAF" modified="2010-06-15" version="1">MTENCCCRHCGARLNDTSKLTLLFLSHFLATPITIRSQVITSTIRSPHSVGLYYSPKKYLLKRATAAPVSSLLQLIYCSMYLNAILSPALGTTTVQSHTKEEIDFVAVLETSQDSRYEQTRWIVQLWYQESEGPWKSADFSPSDGLIPPILSTAAAPNITRRRYSLHLKCRNTNPIQFTLRLKSVLRENAPWIWSNEQNGLGDGRVIFLGPSTGLPTFKSLFHGSDSNIEATCAKSQVPEVELFDVTAPAPPITNGSSTIALGIPAGLELYYALVKLSSPWMGPRQGNSHFSIDLDGLLTGFLRSDGRHVVVLGVSGINGCTTYVRSEAGKVLLKTRNDAGNSQHHRAIIATGWKYQDAVNAAFYRARELIQALTPLSNTVKKSSSIPSWYETWYDGLAYCTWNGLGRELSEEKLLSALQELTDTGIYVTTLIIDDNWQSLRDGSRWDMFEANSKFPLGLGHTTSEIRRRFRNIRHIAVWHSLFGYWDGIAPGGWIDTNYKCINVKWRGGKDICVVDASDVALMYNDFYSFLSKNGIDSIKCDAQYGIDDFDDPKVRQSLGPAYQEAFKINSLKYFSRRVIYSMAHIPYILFRELLPHDASRVLFRNSDDFFPDIPSSHVWHVFANSMNNIYTSNLNCLPDWDMFQSALPTYAGFHAAARCISGGPIYITDTPGHHNISLIKQISAYSPQGYTVALRPSCISLPTDPFVAYNSNRLLKVGNFSGGRGGSSILAVFNVSESQNSELIPMDDFPGLLPGYTYVIRAHTSGGVTAVTPGTGSLMPITLPQYGWELLTAVPVVEITHLKSTGHFTFGVLGIISTMAGVSAIIQQSVNIGLAHITVTITLKALGTIGLYISDLASQDITKFLVTIAGNVVPFHTVKKSSQSNTVLEIDLETAWNELNLSTGWSNAIVIGFHLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>D5GG11</accession>
    <name>D5GG11_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007147001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430231">
        <property type="protein sequence ID" value="CAZ83454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839263.1">
        <property type="nucleotide sequence ID" value="XM_002839217.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GG11"/>
    <dbReference type="GeneID" id="9181799"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007147001"/>
    <dbReference type="HOGENOM" id="CLU_2777734_0_0_1"/>
    <dbReference type="InParanoid" id="D5GG11"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="7"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="69" mass="7133" checksum="0611F7D33CA2079D" modified="2010-06-15" version="1">MSEKHTSGAASTTSTSTTSTIKSAFQKLLAKAPSSSPVRDPGTDMAGPGPAEEKTRALEARVAYLAHRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGA1</accession>
    <name>D5GGA1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007288001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430253">
        <property type="protein sequence ID" value="CAZ83544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839353.1">
        <property type="nucleotide sequence ID" value="XM_002839307.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGA1"/>
    <dbReference type="FunCoup" id="D5GGA1">
        <property type="interactions" value="164"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GGA1"/>
    <dbReference type="GeneID" id="9184606"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007288001"/>
    <dbReference type="eggNOG" id="KOG3433">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080628_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGA1"/>
    <dbReference type="OMA" id="YSMESWI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040453">
        <property type="entry name" value="Mnd1_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003034">
        <property type="entry name" value="SAP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03962">
        <property type="entry name" value="Mnd1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50800">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAP" evidence="1">
        <location>
            <begin position="93"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="177" mass="19767" checksum="B420AB539C6EDEFE" modified="2010-06-15" version="1">MPPKSLSPAKLSQVLAHIHSLNTAHTLKDLEKIIPAATGVSGMLVKDYLTALVDEGLLRVEKIGSGNWYWAFRSEETNTKRRVLKNCLEEKEKLCLAISELKRAIGEIGADEGGEKEELVGRVQELKVVRAGIAAELMGYEEGNVSRLKREIDEIKARVNLTIGKSHPRRRRFRLIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGS6</accession>
    <name>D5GGS6_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">DNA polymerase epsilon catalytic subunit</fullName>
            <ecNumber evidence="17">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">GSTUM_00007495001</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">DNA polymerase II participates in chromosomal DNA replication.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 17">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 17">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 17">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="17"/>
    <dbReference type="EMBL" id="FN430275">
        <property type="protein sequence ID" value="CAZ83698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839507.1">
        <property type="nucleotide sequence ID" value="XM_002839461.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5GGS6">
        <property type="interactions" value="663"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GGS6"/>
    <dbReference type="GeneID" id="9187258"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007495001"/>
    <dbReference type="eggNOG" id="KOG1798">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000556_0_1_1"/>
    <dbReference type="InParanoid" id="D5GGS6"/>
    <dbReference type="OMA" id="MLDQCRY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008622">
        <property type="term" value="C:epsilon DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008310">
        <property type="term" value="F:single-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006287">
        <property type="term" value="P:base-excision repair, gap-filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045004">
        <property type="term" value="P:DNA replication proofreading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006297">
        <property type="term" value="P:nucleotide-excision repair, DNA gap filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05779">
        <property type="entry name" value="DNA_polB_epsilon_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05535">
        <property type="entry name" value="POLBc_epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.60:FF:000003">
        <property type="entry name" value="DNA polymerase epsilon catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000010">
        <property type="entry name" value="DNA polymerase epsilon catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1600.10:FF:000006">
        <property type="entry name" value="DNA polymerase epsilon catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="entry name" value="DNA polymerase family B, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.342.10">
        <property type="entry name" value="DNA Polymerase, chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006133">
        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013697">
        <property type="entry name" value="DNA_pol_e_suA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029703">
        <property type="entry name" value="POL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055191">
        <property type="entry name" value="POL2_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054475">
        <property type="entry name" value="Znf-DPOE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10670">
        <property type="entry name" value="DNA POLYMERASE EPSILON CATALYTIC SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10670:SF0">
        <property type="entry name" value="DNA POLYMERASE EPSILON CATALYTIC SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08490">
        <property type="entry name" value="DUF1744"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22634">
        <property type="entry name" value="POL2_thumb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22912">
        <property type="entry name" value="zf-DPOE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23250">
        <property type="entry name" value="zf_DPOE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01159">
        <property type="entry name" value="DUF1744"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 17" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 17" id="KW-0235">DNA replication</keyword>
    <keyword evidence="14 17" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11 17" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="12 17" id="KW-0408">Iron</keyword>
    <keyword evidence="13 17" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 17" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15 17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 17" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 17" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNA polymerase epsilon catalytic subunit A C-terminal" evidence="19">
        <location>
            <begin position="1459"/>
            <end position="1851"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="18">
        <location>
            <begin position="19"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU365029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM01159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CAZ83698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="2165" mass="246826" checksum="C17797984AEBCEBF" modified="2010-06-15" version="1">MYSSRGGSSSTRFKRGGYRRGGNSGGGRFQKTRTITANSLLATDGTSGEEKFESLRLANDIDERMGFARFEAGPKKVGWLVNMKSTILEDGQVTGGKAGVDYYFLDDYGGSFKATVLYDPYFLIATKRGNEPEVEEWLRRKFEGIIKKTSRLTKEDLRMPNHLLGYRRTFIKLEFVNVTNLLAARKVLMPIAEKNRKNVNAMDAYAEVASASAGFDFIDEGNDLRRSTNMVEACDFIIDIREYDVPYHVRVAIDQEIRIGKWYTVEAKHGIVSLTCIEDRLKQAEPVVLAWDIETTKLPLKFPDASIDQIMMISYMIEGSGFLITNREIVSQDIEDFEYTPRPEYEGHFMVFNEPDEKAVLERFFDHIKEAKPTVMVTYNGDFFDWPFVEARASQLGIDVYREIGFRKNSEDVYYSNYSAHMDAFAWVKRDSYLPQGSQGLKAVTTAKLGYDPDELDPELMTRYAMEKPQVLAEYSVSDAVATYYLYMKYVHPFVFALCTIIPLGPDDVLRKGTGTLCEMLLMVQAYRKEIVLPNKHKQVEEAFFDGHLLGSETYVGGHVESIEAGVFRSDIPANFVIDTGAIDKLIEDLDSALQFSIVVEGKKMEDVTNYDEVKEQIRSKLQQLRDLPARNERPSIYHLDVASMYPNIMTTNRLQPDSMISESDCAACDFNRPGKTCDRRLPWAWRGEYLPAKRDEYNMIRYSLENEKFPPKHPKSPQRTWDDLTSEEQASAIKKRLADYSKKVYHKIHETKTIEKEAIICQRENPFYVDTVKNFRDRRYDFKGKQKAWKRKADDLKASGATAAEIDEANKMTVLFDSMQLAHKVILNSFYGYVMRKGSRWYSMEMAGVTCLTGAHIIQLAKEIVERIGRPLELDTDGIWCILPGTFPENFAFTLEGGKKLPISYPCVMLNHLVHAKFTNHQYSELVDPATHRYKTHSENTIFFEVDGPYRAMILPTSKEEDKNLKKRYAVFNHDGSLAELKGFEVKRRGELKLIKIFQSQIFKFFLEGTSLEECYGAVAKTANRWLDVLHLRGRTLADEELIDLICENRSMSKTLAEYGSQKSTSITTAKRLAEFLGEQMVKDKGLNCKYIVSAKPRNAPKVDNPVPRVPHPDWLQRRIDIEDDRFKQKKMTDMFQKRTELEIVPPGVSMHLDDMEDFGGTTTMPLNRPMLATAKRLGKRKAPDTPAPVGGDPFASLPAVMPPMTEDYKGWLQYQKKKWKIQKHARTRRRQLFGDRSSLQADSIGGFFRNQTEFMLSNTWEILQLRPTDMPGEVRAFILVGRKIITLKIKVPRQIFINFKSEELPDVTVKGCIVERVNHTLPNGHPSGNLFKLVVPEDTYISEQKAFSIIFSHASVEGVYETQVPMSIRALLELGNSCTFDESQRGVLGKGLDHGFDLSSLKSKEAKRPYLQIGGMDYLFLYHVVAGDRQVFGLFPPGGEAHVVVYNATREAQGLPNMDKIYAEQFTRRMETANGPGDSMIEYPATLHFQTTYVNTKKKLEKELTDLLKGIRNEESKPTTLVIQSPQQRTLVDAVSILSEFPILTLRSDPADNSLPLGWQTFVAKRIVTHYLALGAWILHLVELARYGRIPLCNLERDDPTFLIDIDYARRLQKNNVVLWWSPSPKPDHGGYEKDDILGSMETVDMPAINNPGSYSSVCIEIEVRNLAINTILTSSLINELEGSDSVAMNPAAPQGDDPTDGTNIIFANNAFTTAGVAVLREMVKAWSALATRGQPVAEIMVEHLVRWVESPDSFLYDRALHYYVQTMSKKAFLQLLTDFRRVGSHIVFANVNKLLVQTTKTDVGNAYAYSEYIIKAIRAKPLFNFLDLKINEYWDYLVWYDEMNHGGKACEKIVEAENQELNIIMHWQFAKFLPQTLQTIFHDWVVEFVEAIHAQKNPSASGPNASTPWPTQIFFKMSEDSGDKVILGNEFKKPLRKQIGQLIRRQQESMIHPEIDVDFEFPSLPGAYRDMKNPVLQLVRSLTAVFGLAKEINTEARILRKELLALLDIKEFSKEGTFENPSETLKFSQVICKNCALPRDMDLCKDEDLMPEVTADGQLRGRAWKCKFDFCGGEFDRLAFEEKMISQLHQFVTAYNVQDLKCGKCQTLKVNEFMEHCSCSGPWVLTAEREVLVKRLHVYKSVAEHYSFRMLEGVVGDIMGCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGW7</accession>
    <name>D5GGW7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00007554001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430286">
        <property type="protein sequence ID" value="CAZ83760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839569.1">
        <property type="nucleotide sequence ID" value="XM_002839523.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGW7"/>
    <dbReference type="FunCoup" id="D5GGW7">
        <property type="interactions" value="11"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GGW7"/>
    <dbReference type="GeneID" id="9181731"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007554001"/>
    <dbReference type="eggNOG" id="KOG4711">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004486_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGW7"/>
    <dbReference type="OMA" id="FWGWAAN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018823">
        <property type="entry name" value="ArAE_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023244">
        <property type="entry name" value="Brefeldin_A-sensitivity_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052430">
        <property type="entry name" value="IVT-Associated"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47804">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47804:SF3">
        <property type="entry name" value="PROTEIN BRE4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10337">
        <property type="entry name" value="ArAE_2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02047">
        <property type="entry name" value="BREFELDNASP4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="242"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="268"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="299"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="683"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="737"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="762"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="800"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="domain" description="Putative ER transporter 6TM N-terminal" evidence="7">
        <location>
            <begin position="163"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="21"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="629"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="652"/>
            <end position="661"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF10337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ83760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="850" mass="94069" checksum="D815D50D55385292" modified="2010-06-15" version="1">MSLRRTLSRHVQLDEQALRRMEHNGRERPSIYSRPRNSQSSFRSGQSRPSRATSGARTPIDPYSPRASVSTLQLQHLLENLELDQYSTYGVEELRDGFFDASFYRPIEQNSDQGGEAEPLLPPEVSTFKRPWSSPRTLIDSLVEDVKFFFRLTFRNEHGFSLAKSFLAYFVGYILCLVPKSQGWLGRYPYWITVATLFNHPGRTLGAQIDATVACSIGAGFGLAVGSLALEVASSTRNAQVGYGGVIAVFWVTFICAASWIRCSLVKLYQLMISVGLAIIFICLVGSEAIDNTGSWDRGILWEFGIPWVVGLGICLIINVIIRPDAGGKAVAAALHRAVISAVEGLVLPRPYSPTAHQNMNLQLVYLSEAIRDMRGEIVISYLRPDNAEQLRNLMQAVIRDIMAIKPDGNLFGHTSPHNTGARGDDSRPSYSIVIEIEPPGARSSGASSLASSTEAHLKIVRDAMEVPARELVSAMTKVLLGCDKELMNYAGHKKLLGSLEDYRDINLRSSRDRLLEAMRVFDESDISLIDHQSLPSAYSAHPELVEMFLFIHPLRQTADSVGNLAGNVLRIVGTQRRKRILLPSYPLQKALYRTNPQVRHDRGGLTAGYYFKSKQNIEKAMETYQAKPFIPSPTSPTVGDEGAPETEPKAMSSNPPTTTEPQTIRYRAWRVLHRLQQWETRFAFKVVFVTIALGIPAWVESSLGGNAQDLITRSITTAVGSAWAGLGHAAGGGDPYVLALFTAIFMVPGMFRFTSSSHPRSGLMGCISYSVVSLSTYTLRDSTVDIPSPAKVAWTRGLSLIVGIVTAVVVNWVIWPFVARHELRKSLSFMMLNLGISYRGVVARYELFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGZ2</accession>
    <name>D5GGZ2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00007626001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430297">
        <property type="protein sequence ID" value="CAZ83785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839594.1">
        <property type="nucleotide sequence ID" value="XM_002839548.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGZ2"/>
    <dbReference type="GeneID" id="9185039"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007626001"/>
    <dbReference type="eggNOG" id="ENOG502QUGJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001743_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGZ2"/>
    <dbReference type="OMA" id="HKTMRPQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000183">
        <property type="term" value="P:rDNA heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030466">
        <property type="term" value="P:silent mating-type cassette heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000059">
        <property type="entry name" value="Similar to transcription factor Zn, GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057725">
        <property type="entry name" value="Ams2-SPT21_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042403">
        <property type="entry name" value="Spt21/Ams2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39147">
        <property type="entry name" value="PROTEIN SPT21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39147:SF1">
        <property type="entry name" value="PROTEIN SPT21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25823">
        <property type="entry name" value="Ams2-SPT21_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GATA-type" evidence="2">
        <location>
            <begin position="510"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="574"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="640"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="930"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="277"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="297"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="329"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="345"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="404"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="421"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="447"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="471"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="575"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="589"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="932"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="959"/>
            <end position="969"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ83785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="992" mass="106464" checksum="D9E01B944BAD29A5" modified="2010-06-15" version="1">MYASRRTAMKVSYAFDDKNKSNCLARWPHLITTRVVELDKDTVVGAVEFKTCILSMISASPELVATLGQDYSVYAYDYSEVGVPLVGCGMLSWAMATVNSNNPSTNASMITGRVVSNSFGFFSNDGVKETLNVKLKLTPVNTFTQAQFVESVHAYNALSKVIPGGFDASAWTNYLNANPHILAPPGGLTSATSKVSHKGATLQPPPSGMGDCNPTSGEENGEPPQKKAKTGPAKARKPSNGNRKKKVAATAAPGASLESIPEMLPLPPTPQVKEDLSPPPPPSQPSPTPTPPELIQTMPNNNPVTPMPTTAFSPVYANSPSRTIDSRTMRSSPPTQLEPSRNEISPTPTSPVLPSLCPDVPSPDGLERRLNALFEEEEEELKSPPVSPEQPITVETTPEEGNPVDNQSMNSSMLELPKISIPTSLPDNVNMSAAPSPDSTRVIAKPKSQKRKYPRKPKRLMSDAAASSELGEERESVKRSRSERPQPKPRTAAHVKERIEMQLVQAVTQGKMPNFCMNCGAIETPTWRKVMIARDDNDEDIKDKGIKTEKGEKEILLCNPCGLWHASHKTMRPQDYWDGKKEEPSAPVKKPRVRKRKQPATAKQMPKEAQQPTSTQLPGPLTGPIVVLDEEETPRHAPATVRKVLAMTPRGTRSTKGSNPEWEAAYEAAKRVVQSSPIKPGTVDSPIDLDGPESKSKSPRRLLFTKPKVNGATNLRPQGSGDGDLTSKSFQGVIAAGDVEKENCAPESLEPRAPSDIAALDKPTTPQKRKVVPLYPRTPIRTGSRTSLGSPSPWNAAIFSSAKKVASGLSPGHRHLSSPQRRNLSPTSAFLEKLLEGADIDTASRNLEEMSTDILGTSPHHHLPGSAFNFGDDIFGTDMTMPSSPPLFNLDGVVDNDTVNNALWSEFLPSTPDMGNMTCGLDIFEDIHGVEGRGGGQGGGAGAGDDDDGDGDGRRRSESSSVVPTPTTSGGRVDFSSFIDDVSKAVAAGIGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>D5GH47</accession>
    <name>D5GH47_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00007700001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430297">
        <property type="protein sequence ID" value="CAZ83840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839649.1">
        <property type="nucleotide sequence ID" value="XM_002839603.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GH47"/>
    <dbReference type="STRING" id="656061.D5GH47"/>
    <dbReference type="GeneID" id="9185094"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007700001"/>
    <dbReference type="HOGENOM" id="CLU_2851351_0_0_1"/>
    <dbReference type="InParanoid" id="D5GH47"/>
    <dbReference type="OMA" id="TRRDFPV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006144">
        <property type="term" value="P:purine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3330.10">
        <property type="entry name" value="Oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018020">
        <property type="entry name" value="OHCU_decarboxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036778">
        <property type="entry name" value="OHCU_decarboxylase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09349">
        <property type="entry name" value="OHCU_decarbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158694">
        <property type="entry name" value="UraD-Like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0659">Purine metabolism</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase" evidence="2">
        <location>
            <begin position="15"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ83840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="65" mass="7592" checksum="F02D74BFB6873A50" modified="2010-06-15" version="1">MRSTRRDFPVNKSFFTNGHGRQIIIKDMERRISRCDIEAEKEEAIKAVCDIARDRANKLTPKKTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5GH94</accession>
    <name>D5GH94_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00007803001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430308">
        <property type="protein sequence ID" value="CAZ83919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839728.1">
        <property type="nucleotide sequence ID" value="XM_002839682.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GH94"/>
    <dbReference type="STRING" id="656061.D5GH94"/>
    <dbReference type="GeneID" id="9182624"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007803001"/>
    <dbReference type="eggNOG" id="KOG2483">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_763311_0_0_1"/>
    <dbReference type="InParanoid" id="D5GH94"/>
    <dbReference type="OMA" id="HIGATHH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10328:SF15">
        <property type="entry name" value="BHLH TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10328">
        <property type="entry name" value="PROTEIN MAX MYC-ASSOCIATED FACTOR X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BHLH" evidence="4">
        <location>
            <begin position="302"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="18"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="247"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="22"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="41"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="88"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="130"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="148"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="259"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="281"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ83919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="363" mass="38681" checksum="F6C15C1FC036955C" modified="2010-06-15" version="1">MRSTLSYRGAKVVRARLEHNQHGSTRQHAQDTSNPPFSYNHSPTSSTFAASTTSLHTTPPAGVYQATDVLPAPSPSASRRSSFDSRLGNLNLSSPVNGTPAVSQVSLASTLQRERGIHSNSPASHRAENGDTNGSSTSGPYGGIARRYTSTTDSSNGYTPSHKSNGPTRVAPPIIGAPRFPYINGPHPHPNAPSPTKGYPYAFPDPDFAGPPSRSSRDGIPGRNSTSSLVGSTASSIYSTNSGLLHPNLADNGNGNFSGGQHAQAANGNQHHHHHLQTRKPSAGASGDSASPPVTPYSRTPELRVSHKLAERKRRKEMKDLFDELRDQLPAERGGKSSKWEVLTKGRLQFLAIHATIRGCSRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHB0</accession>
    <name>D5GHB0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00007729001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 4-hydroxybenzoyl-CoA thioesterase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430308">
        <property type="protein sequence ID" value="CAZ83862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839671.1">
        <property type="nucleotide sequence ID" value="XM_002839625.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHB0"/>
    <dbReference type="STRING" id="656061.D5GHB0"/>
    <dbReference type="GeneID" id="9182567"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007729001"/>
    <dbReference type="eggNOG" id="ENOG502S411">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101141_0_1_1"/>
    <dbReference type="InParanoid" id="D5GHB0"/>
    <dbReference type="OMA" id="DNDAYGH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047617">
        <property type="term" value="F:fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00586">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000104">
        <property type="entry name" value="Thioesterase family protein (AFU_orthologue AFUA_2G16350)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050563">
        <property type="entry name" value="4-hydroxybenzoyl-CoA_TE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31793">
        <property type="entry name" value="4-HYDROXYBENZOYL-COA THIOESTERASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31793:SF27">
        <property type="entry name" value="NOVEL THIOESTERASE SUPERFAMILY DOMAIN AND SAPOSIN A-TYPE DOMAIN CONTAINING PROTEIN (0610012H03RIK)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioesterase" evidence="3">
        <location>
            <begin position="34"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ83862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="171" mass="19062" checksum="FB75549FA4EF2511" modified="2010-06-15" version="1">MSSVKSVKSVKSRTRSDYGYFLTYRTRWSDNDIYNHLNNVVYNILIDSVVNSYLITHCGMMPTTSPSIGLMVSSSCTFVSPCAFPAVLELGLRVNKLGTSSVTYEVGIFEEGVEGVKAVGGFTHVFCDSVHRRPMKLLRPYREGLESLLVRAGGEEEGDEEETVRYRSAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHI1</accession>
    <name>D5GHI1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007927001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the asaB hydroxylase/desaturase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430319">
        <property type="protein sequence ID" value="CAZ83974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839783.1">
        <property type="nucleotide sequence ID" value="XM_002839737.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHI1"/>
    <dbReference type="GeneID" id="9183628"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007927001"/>
    <dbReference type="HOGENOM" id="CLU_042688_2_0_1"/>
    <dbReference type="InParanoid" id="D5GHI1"/>
    <dbReference type="OMA" id="DMAMTDG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044053">
        <property type="entry name" value="AsaB-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041278">
        <property type="entry name" value="CmcJ_NvfI_EfuI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34598">
        <property type="entry name" value="BLL6449 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34598:SF3">
        <property type="entry name" value="OXIDOREDUCTASE AN1597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="306" mass="35783" checksum="0706A5BB496E1028" modified="2010-06-15" version="1">MDESAQFFYLKFLPLYEIEKPYHNFTDGASNITTEKRPLQPVLDIRGREREFTLAIHGFEYRTRALPVVNWKDEEEIKKTYVDDLKVFVHELVPETVQRCEMFDFRRQLRSVEAIQSKIPIHPGLCGTYKLSPALLVHIGESLLMSGKWGGGGWDYMHVADQTPEGVLEWLKRELGREETAEIVKKYRVRVINIWRPLVDVVEDNPLAMCDARTVKASDLVSSVHVSSDYTRRNYIVKYSPDFKFYYLSKMMKEEVCAFMVFDSCGVGEERIRTPPHSAFWHSEKWRSDKYPRESIEVRMLVLSAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHQ9</accession>
    <name>D5GHQ9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nucleotide exchange factor SIL1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00007999001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Interacts with KAR2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIL1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430320">
        <property type="protein sequence ID" value="CAZ84020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839829.1">
        <property type="nucleotide sequence ID" value="XM_002839783.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHQ9"/>
    <dbReference type="FunCoup" id="D5GHQ9">
        <property type="interactions" value="131"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GHQ9"/>
    <dbReference type="GeneID" id="9183674"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007999001"/>
    <dbReference type="eggNOG" id="KOG2160">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034955_0_0_1"/>
    <dbReference type="InParanoid" id="D5GHQ9"/>
    <dbReference type="OMA" id="GLDIRMN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050693">
        <property type="entry name" value="Hsp70_NEF-Inhibitors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031884">
        <property type="entry name" value="Sil1_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19316:SF34">
        <property type="entry name" value="NUCLEOTIDE EXCHANGE FACTOR SIL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19316">
        <property type="entry name" value="PROTEIN FOLDING REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16782">
        <property type="entry name" value="SIL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072795" description="Nucleotide exchange factor SIL1" evidence="10">
        <location>
            <begin position="22"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="100"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ84020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="424" mass="46347" checksum="735F09EEB096F529" modified="2010-06-15" version="1">MRSLLLPCLVAAPAFVTAVTGQRTHNQPKHALHEDSPDLICHSGTCYPRVFQPTHEFQVVHDDQELPRGLHYRLNLETGLKEAKINVDDSSETISGVVVLPDQGAESLPPDDHSRQRVPSSDDGAIKPPSSAPGDSVTLSESLEIILDTSSRSTPDIIAALLSLEDSAHEIYWGLQISAPRYVASLLRLISSAPDASVRSSSALVLGNALSNNPKALSSATSDPLVPLIRSLLSSLGTENDTGAKARILYALNKAIKSPLTRSEFLIGGGMRTLQDLFAQGNAEFMAKTATFVEDNFLNDDMRAESELKAEPGVKKQDNIPNRYRPDRVLNDERSFYRPFEDALLNRSNQKDLENDEFKSKVFSALSALKRKHPGDEACTPSANFLIWLEGQADLRGKTLLELEEDGDQLTKVLAVENRGLFVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHW1</accession>
    <name>D5GHW1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00008143001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 81 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="3"/>
    <dbReference type="EMBL" id="FN430321">
        <property type="protein sequence ID" value="CAZ84104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839913.1">
        <property type="nucleotide sequence ID" value="XM_002839867.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHW1"/>
    <dbReference type="FunCoup" id="D5GHW1">
        <property type="interactions" value="204"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GHW1"/>
    <dbReference type="GeneID" id="9184197"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008143001"/>
    <dbReference type="eggNOG" id="KOG2254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005482_2_1_1"/>
    <dbReference type="InParanoid" id="D5GHW1"/>
    <dbReference type="OMA" id="DTMFAYA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052861">
        <property type="term" value="F:endo-1,3(4)-beta-glucanase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1170">
        <property type="entry name" value="glycoside hydrolase family 81 endo-[beta] glucanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.30">
        <property type="entry name" value="Golgi alpha-mannosidase II, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005200">
        <property type="entry name" value="Endo-beta-glucanase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040720">
        <property type="entry name" value="GH81_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040451">
        <property type="entry name" value="GH81_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31983">
        <property type="entry name" value="ENDO-1,3(4)-BETA-GLUCANASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31983:SF0">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-D-GLUCOSIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17652">
        <property type="entry name" value="Glyco_hydro81C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03639">
        <property type="entry name" value="Glyco_hydro_81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52008">
        <property type="entry name" value="GH81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 81 N-terminal" evidence="9">
        <location>
            <begin position="39"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 81 C-terminal" evidence="10">
        <location>
            <begin position="373"/>
            <end position="723"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ84104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="733" mass="82638" checksum="CB390A85D236EBD4" modified="2010-06-15" version="1">MSDFDGRGGRLDRNQLVDLFSEPISTKVPSQQFSSFSEHPVRRSFFPKTSSREPLPTNKFYSNLFLGNQTQGVWTFPYMIWWNKTGQIPGLSVSHTEQHQFVTGPVPSADPYQYYISPLGIASITFSAKEFAERHSNISLTNPHSRSVNLNLHANTNKYGPIGHKITFPLVQGMGFITGRYDQLTPIISSYSEFVKIEAFSGPYNGTQKWRLGLSDGRMWLLYVFPAVGSESPEFTYNPGEGLVGSHEFTGVIQIAKADAEGQKGGLEEITDSAAGVYPATVEMTGGVVGDVGSYKFEYKGWRDEECEGALMYALPHHIASFDDNTLRGRTGLKLQSPTKGLMEAVRGNSWTMVERNLPTSVAWLATRGQRLNRHTTELIERTLVSEIKSNIIGETNLNSMYFSGKAGCSRPNIPHDVVKKENLIREALGKLITALDPYVNNRQNQPLVYDETWKGLITSAIKQGQMADFGNGMYNDHHFHYGYFVYTAAVIVRLEQSLNKGKSPWLERNRDWVDILIRDVANPSTLDPYFPESRSFDWFHGHSWAKGLFESADGKDQESSSEDMNYGYSQKLWGKVTGDKSMEARADLMLSVMKRSFNQYFLLKSGSRNHPPGFINNKVTGILFENKVDHTTYFGHVNERIHGIHMIPITPISPWYRDPVFCREEWETWFSNGRVNKSEGGWNGILQANRAIFDPKGSWAFFTNPDFVKEYLDPGASKSWYLAFVAGLGGED</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHW5</accession>
    <name>D5GHW5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00008150001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430321">
        <property type="protein sequence ID" value="CAZ84108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839917.1">
        <property type="nucleotide sequence ID" value="XM_002839871.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHW5"/>
    <dbReference type="STRING" id="656061.D5GHW5"/>
    <dbReference type="GeneID" id="9184201"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008150001"/>
    <dbReference type="eggNOG" id="ENOG502S2W1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040908_3_2_1"/>
    <dbReference type="InParanoid" id="D5GHW5"/>
    <dbReference type="OMA" id="WYDNFAG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071966">
        <property type="term" value="P:fungal-type cell wall polysaccharide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000207">
        <property type="entry name" value="Glycoside hydrolase family 128 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053183">
        <property type="entry name" value="ASL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024655">
        <property type="entry name" value="Asl1_glyco_hydro_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34154">
        <property type="entry name" value="ALKALI-SENSITIVE LINKAGE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34154:SF3">
        <property type="entry name" value="ALKALI-SENSITIVE LINKAGE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11790">
        <property type="entry name" value="Glyco_hydro_cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072745" evidence="1">
        <location>
            <begin position="18"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="Asl1-like glycosyl hydrolase catalytic" evidence="2">
        <location>
            <begin position="34"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ84108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="298" mass="33065" checksum="A947FCB19C5FE4F3" modified="2010-06-15" version="1">MFLTPLLFLLLTPTIIAQTTSSKRGLVHIPSSDHPGDDSIWITPPTTLTWYYNYGSRPSSSLSSGNLSFVPMLWGDHSNTFVDDVRGQKGVKWVLGFNEPDMGSNVGGSNIRPERAAELWKRDIEPLKGDGKELGAPSISGSPTGMRWLKEFFKACNGCSVDFIPIHWYGNFEGFASHVGEVRATFPNKTIWVTELGWADQPLASTQSFFNATLKYLDRLDYVTHYSWFGSFRSDVSNVGPNGAMLNSAGKLTDIGAWYLGRQGEGIKPDTNNSAARVGRGDWVLWRIVYFIISVRAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHX7</accession>
    <name>D5GHX7_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">acetylornithine transaminase</fullName>
            <ecNumber evidence="5">2.6.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00008176001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 4/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.11" evidence="5"/>
    <dbReference type="EMBL" id="FN430322">
        <property type="protein sequence ID" value="CAZ84120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839929.1">
        <property type="nucleotide sequence ID" value="XM_002839883.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHX7"/>
    <dbReference type="FunCoup" id="D5GHX7">
        <property type="interactions" value="248"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GHX7"/>
    <dbReference type="GeneID" id="9184213"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008176001"/>
    <dbReference type="eggNOG" id="KOG1401">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016922_10_1_1"/>
    <dbReference type="InParanoid" id="D5GHX7"/>
    <dbReference type="OMA" id="MVPGFKY"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00109"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003992">
        <property type="term" value="F:N2-acetyl-L-ornithine:2-oxoglutarate 5-aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000004">
        <property type="entry name" value="Acetylornithine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01107">
        <property type="entry name" value="ArgD_aminotrans_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004636">
        <property type="entry name" value="AcOrn/SuccOrn_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049704">
        <property type="entry name" value="Aminotrans_3_PPA_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050103">
        <property type="entry name" value="Class-III_PLP-dep_AT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00707">
        <property type="entry name" value="argD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002325">
        <property type="entry name" value="PRK01278.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986:SF79">
        <property type="entry name" value="ACETYLORNITHINE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986">
        <property type="entry name" value="AMINOTRANSFERASE CLASS III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="9 10" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ84120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="464" mass="50408" checksum="F527A074A8DDF52F" modified="2010-06-15" version="1">MALRQASRKPLRTWLIRTPIIRGFASVSNLPKSVKDEIAHEQLLPNYDPPTDSATGSLLSKQAPYMVRTYVRPPQMFVRGVGSVLYDVENRKYIDFTAGIAVNSLGHGDEHLCNLIFDQAKQVIHTSNLYHNPWTGELAALLVNKTKEHGAFHNASQVFVCNSGSEANEAALKFARKVGKVIGGEKKIGVVSFTNSFHGRTMGALSATPNPKYQKPFSPMVPGYTHGVYNDIAGINDLVTGETCGVIVEPIQGEGGVNVGSPEFLIALSKRCKEVGAVLIYDEIQCGLGRTGDLWAHQFLPKEAHPDILTMAKALGNGFPIGATMVNNFVGEKIMTGDHGTTFGGNPLASRIGHHVFSRLSEHSLLKGVEAKSGVFVKRLTAIKDKYPEVATEVRGKGLILGLQLTRDPTSLVKEARERGLLIITAGTNTVRFVPPLVIEEEIINEGLDIFEKAMKVFVESGNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>D5GI20</accession>
    <name>D5GI20_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">26S proteasome regulatory subunit 6B homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00008234001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430322">
        <property type="protein sequence ID" value="CAZ84163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839972.1">
        <property type="nucleotide sequence ID" value="XM_002839926.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GI20"/>
    <dbReference type="FunCoup" id="D5GI20">
        <property type="interactions" value="1211"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GI20"/>
    <dbReference type="GeneID" id="9183496"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008234001"/>
    <dbReference type="eggNOG" id="KOG0727">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_2_0_1"/>
    <dbReference type="InParanoid" id="D5GI20"/>
    <dbReference type="OMA" id="QDIGGMD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045899">
        <property type="term" value="P:positive regulation of RNA polymerase II transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070682">
        <property type="term" value="P:proteasome regulatory particle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd19502">
        <property type="entry name" value="RecA-like_PAN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000020">
        <property type="entry name" value="26S protease regulatory subunit 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000049">
        <property type="entry name" value="26S protease regulatory subunit 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000033">
        <property type="entry name" value="26S protease regulatory subunit 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032501">
        <property type="entry name" value="Prot_ATP_ID_OB_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16450">
        <property type="entry name" value="Prot_ATP_ID_OB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0647">Proteasome</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="13">
        <location>
            <begin position="190"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="50"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00068703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ84163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="413" mass="46224" checksum="E85C5F62A40BE42B" modified="2010-06-15" version="1">MGDVAVQKRPAILPLTDPSYGTLDGSLGDNNDYAKLKRLQRHLEYITLQEEYIKDEQRSLKRELVRAQEEIKRIQSVPLVIGQFMEAIDQNTGIVQSSTGSNFVVRILSTLDRELLKPSSSVALHRHSNSLVDILPPEADSSIAMLGAHEKPDVTYADVGGLDMQKQEIREAVELPLTHFDLYKQIGIDPPRGVLLYGPPGTGKTMLVKAVANSTTASFIRVVGSEFVQKYLGEGPRMVRDVFRMARENSPAIIFIDEIDAIATKRFDAQTGADREVQRILLELLNQMDGFDQTSNVKVIMATNRADTLDPALLRPGRLDRKIEFPSLRDRRERRLIFTTIASKMSLSPEVDLDSLIIRNDPLSGAIIAAIMQEAGLRAVRKNRYNIIQADLEDAYSSQVKGGNEADKFEFYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5GI57</accession>
    <name>D5GI57_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">histidinol-phosphate transaminase</fullName>
            <ecNumber evidence="4">2.6.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Imidazole acetol-phosphate transaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00008285001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-histidinol phosphate + 2-oxoglutarate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:23744"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57766"/>
            <dbReference type="ChEBI" id="CHEBI:57980"/>
            <dbReference type="EC" id="2.6.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 7/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.9" evidence="4"/>
    <dbReference type="EMBL" id="FN430323">
        <property type="protein sequence ID" value="CAZ84200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840009.1">
        <property type="nucleotide sequence ID" value="XM_002839963.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GI57"/>
    <dbReference type="FunCoup" id="D5GI57">
        <property type="interactions" value="168"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GI57"/>
    <dbReference type="GeneID" id="9181918"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008285001"/>
    <dbReference type="eggNOG" id="KOG0633">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_3_1_1"/>
    <dbReference type="InParanoid" id="D5GI57"/>
    <dbReference type="OMA" id="NFVQFGR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004400">
        <property type="term" value="F:histidinol-phosphate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01023">
        <property type="entry name" value="HisC_aminotrans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001917">
        <property type="entry name" value="Aminotrans_II_pyridoxalP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005861">
        <property type="entry name" value="HisP_aminotrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01141">
        <property type="entry name" value="hisC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42885:SF2">
        <property type="entry name" value="HISTIDINOL-PHOSPHATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42885">
        <property type="entry name" value="HISTIDINOL-PHOSPHATE AMINOTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00599">
        <property type="entry name" value="AA_TRANSFER_CLASS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="9" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="12">
        <location>
            <begin position="37"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ84200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="402" mass="43760" checksum="0573449C21EE4733" modified="2010-06-15" version="1">MAPTSAFNLQTCARKNILQLVPYRCARDDYKDDGSNILLDANENAYGPSLPFNCPDESEAGIGYDYTHLNRYPDPHQEDLKRLFCNYRNKNPTKPLTPANLFLGVGSDEAIDAVLRCFCIPGRDKILTCPPTYGMYSVSAQVNDVEVIREPLDLVSGEFELRPDAINKRLSEDPTIKLVYICSPGNPTAGTVDPEAVRKVLAHPTWNGVIVVDEAYIDFAPEGSSLAPCVVDHPNLVVMQTLSKAFGLAGIRLGAAFTAPEIARLLNNLKAPYSVSSTTSSLAMAALSPAGLAVKNENVRRLLVQRDRIISELPELPGIGRFLGGFDSNFLLVEILDNNAVPNSGVAKKVYEQLAEEHGVIVRYRGGEAGCTGCLRITVGTEAEVGRLLKKLRETLNAVRGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIA4</accession>
    <name>D5GIA4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="12">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00008366001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tim17/Tim22/Tim23 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430324">
        <property type="protein sequence ID" value="CAZ84247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840056.1">
        <property type="nucleotide sequence ID" value="XM_002840010.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIA4"/>
    <dbReference type="FunCoup" id="D5GIA4">
        <property type="interactions" value="1102"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GIA4"/>
    <dbReference type="GeneID" id="9181965"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008366001"/>
    <dbReference type="eggNOG" id="KOG1652">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087811_3_0_1"/>
    <dbReference type="InParanoid" id="D5GIA4"/>
    <dbReference type="OMA" id="FDCTFQY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10485:SF0">
        <property type="entry name" value="AT05822P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10485">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM-17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02466">
        <property type="entry name" value="Tim17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="143"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="153"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ84247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="162" mass="16443" checksum="25626E083A519D02" modified="2010-06-15" version="1">MRQSNLVDHSRDPCPWVVLSDFGGAFAMGAVGGAIWHGVKGFKNSPSGERSIGALTSIKARAPVLGGNFGVWGGLFSSFDCAVKGVRQKEDPYNAIIAGFFTGGALAVRGGVKAARNSAIGCACLLAVIEGVGIGLNRVTAGGSRPQNPGLPSESSTEALPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIN3</accession>
    <name>D5GIN3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00008582001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430327">
        <property type="protein sequence ID" value="CAZ84376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840185.1">
        <property type="nucleotide sequence ID" value="XM_002840139.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIN3"/>
    <dbReference type="STRING" id="656061.D5GIN3"/>
    <dbReference type="GeneID" id="9183310"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008582001"/>
    <dbReference type="eggNOG" id="KOG3130">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030204_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIN3"/>
    <dbReference type="OMA" id="LNARGMW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019212">
        <property type="term" value="F:phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024325">
        <property type="entry name" value="DUF3835"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039553">
        <property type="entry name" value="Prefoldin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052255">
        <property type="entry name" value="RNA_pol_II_subunit5-mediator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15111">
        <property type="entry name" value="RNA POLYMERASE II SUBUNIT 5-MEDIATING PROTEIN NNX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15111:SF0">
        <property type="entry name" value="UNCONVENTIONAL PREFOLDIN RPB5 INTERACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12927">
        <property type="entry name" value="DUF3835"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13758">
        <property type="entry name" value="Prefoldin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3835" evidence="3">
        <location>
            <begin position="527"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="179"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="301"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="354"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="429"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="6"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="219"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="308"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="354"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="394"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="439"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ84376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="607" mass="67052" checksum="526942BF29857791" modified="2010-06-15" version="1">MPIEVERSLELHRQRLEASLEKLRKALRHWQISSAEYEALREELQVLPGDATREDMLKVGSGFGGTVVDEEDVKALLGDNVGIRRTVDQAIAAVGHRINYVQENVDKVQKQIDQGETELAPLLVVNEPDLKAEDGLPIMDIREELDEDGNIINTTISGADPSSAKALDTTPLEQLQKMVGTDEDAKEQKLPVEASEETSENKPAEASKPSPQVPSPSARKSDKTPAKGELDGVKTISPKDEEGGGFVRVLDDPVLNEEDFVKEVPTDSPEEAEMRKQMLNYNLQEIGAVVAEINLEDEGIYYGHEGDSNEETDELDSEDEDRYGRTATRVITLEYQKEMEDLARKIKERGVVARAAEAKLREEESQRRKDEAKTRKPKKKKGVRFATEPEISTAPPPMPDFPAPPSAHNTEDALPLLVDLLVMDAMRRSGTVPGNTAPVKEKGPSIFKSEKTKVQNKKIEETPERVVPTSVISLDVMERSADDGPTSLEPPASKSRKVSRFKAAMAIPQDDDVDNDASPPKTSTNGSVIEREVSESAPVQPPDELNPLTHRSEVAVEYHRLRNKMIQQQGVFVGSGEEMAKVLLDDNGETQKVSRFKAARMKGLLPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIQ5</accession>
    <name>D5GIQ5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00008609001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430328">
        <property type="protein sequence ID" value="CAZ84398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840207.1">
        <property type="nucleotide sequence ID" value="XM_002840161.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIQ5"/>
    <dbReference type="STRING" id="656061.D5GIQ5"/>
    <dbReference type="GeneID" id="9183332"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008609001"/>
    <dbReference type="eggNOG" id="ENOG502S7ZI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056562_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIQ5"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14705">
        <property type="entry name" value="bZIP_Zip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000075">
        <property type="entry name" value="BZIP transcription factor (MetR)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13044">
        <property type="entry name" value="ACTIVATING TRANSCRIPTION FACTOR ATF 4/5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13044:SF14">
        <property type="entry name" value="CRYPTOCEPHAL, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07716">
        <property type="entry name" value="bZIP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="7">
        <location>
            <begin position="179"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="108"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="236"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="131"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="171"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="199"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="256"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ84398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="289" mass="31438" checksum="D4A4F773746870DE" modified="2010-06-15" version="1">MAGTYSGRRSGVNVSSYIENLNSITADEPLPDLQDDLALFTNISWIDYDMCRPLDDGTSDALKRSEDFAALDQKPSDEQMNFMNDIDSSFVLSKFALDGALETQPTTDIVNPFAPDGTSSIGPVDSVMHSPASNSPTAYPQANNGTRTSAKRKGSAVSVAYTSPQADSFEEASRLAAEEDKRRRNTAASARFRVKKKQREQALERSAKEMSEKVRLLEKKIVQLETENKWLKSLIVEKQTPNSGKGAQDPSEDFAAKSPAAMKAGASSNEPNTNQNKDGVGTMPEVNSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIW9</accession>
    <name>D5GIW9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008721001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840271.1">
        <property type="nucleotide sequence ID" value="XM_002840225.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIW9"/>
    <dbReference type="GeneID" id="9185680"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008721001"/>
    <dbReference type="HOGENOM" id="CLU_3126097_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIW9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061928">
        <property type="term" value="F:glutathione specific gamma-glutamylcyclotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006751">
        <property type="term" value="P:glutathione catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006840">
        <property type="entry name" value="ChaC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04752">
        <property type="entry name" value="ChaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="50" mass="5783" checksum="B0D15D5E689E856C" modified="2010-06-15" version="1">MNHSTEFWLFGYGSLIWKPPPDYDRRVPGYIKGKLLDTCLMDILGDGAFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJ31</accession>
    <name>D5GJ31_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008812001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJ31"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008812001"/>
    <dbReference type="eggNOG" id="ENOG502SH1E">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052682_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJ31"/>
    <dbReference type="OMA" id="LVHTTNM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="53"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="230" mass="24395" checksum="D5B0FF3D36E64708" modified="2010-06-15" version="1">MAIGRTLTKKPSKYSFSTTSPTEAQSKRGSISKIQISAPLELISTTNMISYTSPSLRSSISHPSPSTSSISTSPTSSRFPPSSPSSSSASSNSGSDSEISSPDEPSPPPNHLSMYFPPAVPARSKSRRTVPPPPPPAHPSPAPVAGLNLTRTPSRSQQKMHSRSVRASNPFGVELAKVAEIAEDIGVQVRDAEEEFMMERGLQKFSANDYAAEISGVFDDVLPNLGAGWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJ59</accession>
    <name>D5GJ59_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00008849001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the POMP/UMP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840361.1">
        <property type="nucleotide sequence ID" value="XM_002840315.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJ59"/>
    <dbReference type="FunCoup" id="D5GJ59">
        <property type="interactions" value="390"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJ59"/>
    <dbReference type="GeneID" id="9185920"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008849001"/>
    <dbReference type="eggNOG" id="KOG3061">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100687_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJ59"/>
    <dbReference type="OMA" id="MSMRIVP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008012">
        <property type="entry name" value="Ump1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12828:SF3">
        <property type="entry name" value="PROTEASOME MATURATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12828">
        <property type="entry name" value="PROTEASOME MATURATION PROTEIN UMP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05348">
        <property type="entry name" value="UMP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00043974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ84552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="204" mass="23069" checksum="93B2084F21C6561D" modified="2010-06-15" version="1">MPPSYHSTFPQLELHHTSPPSTHNTAFIHRSIYRTAIIDNNPINQSNQTRVSQTSKTEMSLRFIPPATSTPATLPAPTASAPSAASIPDTLRAGGPTSLASTLNSRHPLEARLAQWDETQEKLKMENLRRMFGVQEVIRRGMEIKIAGSDWRPAQLGGPSNFHLDILRGVDERIEWEDVFKGNDNTFEMPDFHVEMERKLRMNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJY0</accession>
    <name>D5GJY0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00009263001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UTP5 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430334">
        <property type="protein sequence ID" value="CAZ84823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840632.1">
        <property type="nucleotide sequence ID" value="XM_002840586.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJY0"/>
    <dbReference type="STRING" id="656061.D5GJY0"/>
    <dbReference type="GeneID" id="9186859"/>
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    <dbReference type="eggNOG" id="KOG4547">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038849_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJY0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007148">
        <property type="entry name" value="SSU_processome_Utp12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052414">
        <property type="entry name" value="U3_snoRNA-assoc_WDR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44267">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44267:SF1">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04003">
        <property type="entry name" value="Utp12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Small-subunit processome Utp12" evidence="5">
        <location>
            <begin position="137"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="263"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="298"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="68"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="267"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="360"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="376"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="395"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038335"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF04003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ84823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="406" mass="43389" checksum="7E5124AB16831E05" modified="2010-06-15" version="1">MVWSSTETSTLALAGHTNISRHAGISATVSGAMNGPRHAVDSSRAAVVRGGDLRDIEIGDLSSGEESAAEKETDREGDGIGDGVEQTFGEKAQALKVSEASKAPRSKATREITNAAVKAPATGSLTTVLTQALQTEDNSLLESCLHSTDDEAILATIRRLNSSLAVILLKRLAERIARHPGRARPLGTWVKWIAVAHGGYLVTLPDLVSQISELHQIVSARASSMPKLLSLQGRLEMLSAQMQFRSSPAGASKQDEDNKVLYMEGTGDSDSESESDDEPENVGLEGFHIEDASFINAEGSTCDDDSANGSETSDSDAELSDAEGYVDSGDDGHGDSEFPNGYRTSDFLDTEAIESSAREEPDEEEPADLDSEVDNGESRDDKGSDYGSEDDQRIVKRIRRSKKASR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5GK67</accession>
    <name>D5GK67_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00009398001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="CAZ84910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840719.1">
        <property type="nucleotide sequence ID" value="XM_002840673.1"/>
    </dbReference>
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    <dbReference type="KEGG" id="tml:GSTUM_00009398001"/>
    <dbReference type="HOGENOM" id="CLU_2456401_0_0_1"/>
    <dbReference type="InParanoid" id="D5GK67"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="89" mass="9967" checksum="40F294CD7EAACE4C" modified="2010-06-15" version="1">MLEWKEGGKERTGRLNRLPGSNRGERTPVLGVERVVVVGTSGNCEPPHISRNPPGAPRHEPTKSSRPSPISSGNTRVLVWVWRPYNFHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>D5GK82</accession>
    <name>D5GK82_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">General transcription and DNA repair factor IIH</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">GSTUM_00009420001</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to TFIIK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module TFIIK controls the initiation of transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the GTF2H2 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430337">
        <property type="protein sequence ID" value="CAZ84925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840734.1">
        <property type="nucleotide sequence ID" value="XM_002840688.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GK82"/>
    <dbReference type="FunCoup" id="D5GK82">
        <property type="interactions" value="953"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GK82"/>
    <dbReference type="GeneID" id="9186954"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009420001"/>
    <dbReference type="eggNOG" id="KOG2807">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028556_2_0_1"/>
    <dbReference type="InParanoid" id="D5GK82"/>
    <dbReference type="OMA" id="INWVEVP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01453">
        <property type="entry name" value="vWA_transcription_factor_IIH_type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000477">
        <property type="entry name" value="General transcription and DNA repair factor IIH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000015">
        <property type="entry name" value="General transcription factor IIH subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007198">
        <property type="entry name" value="Ssl1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004595">
        <property type="entry name" value="TFIIH_C1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012170">
        <property type="entry name" value="TFIIH_SSL1/p44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00622">
        <property type="entry name" value="ssl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12695">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIH SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12695:SF2">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIH SUBUNIT 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07975">
        <property type="entry name" value="C1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04056">
        <property type="entry name" value="Ssl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015919">
        <property type="entry name" value="TFIIH_SSL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01047">
        <property type="entry name" value="C1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="8 12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="VWFA" evidence="15">
        <location>
            <begin position="107"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="13">
        <location>
            <begin position="332"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="30"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR015919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR015919-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAZ84925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="469" mass="50762" checksum="2F0B0ECC8D0D70CA" modified="2010-06-15" version="1">MADSDEDYIATDDEEIGAHNISKSGRHRGAGGAAATGGSGARSKNGSKPGGGAQKRERWEDIKRSWDNVVEGEDGSLSSTVAGLLEAGKRKRLLKDTTPLQRGIIRHLILILDLSSAMMEKDFRPTRYLLSLRYACDFVREYFEQNPISQLGVLGMRDGLAVRISDLSGNPVDHIEAIGKLRLDEGKGAPSLQNALEMSRAALFHAPSHGTREVVIVFGALLSSDPGDIHQTISHLVEDKIRVSVVGLSAQVAVCRELCKRTNAGDEGVYGVALDDKHFRELLLDTTIPPVTRSAKMSASSLLMMGFPSRMVEKTASLCACHSTLTKGGYLCSRCSTKVCALPMECPACGLTLILSTHLARSYHHLFPLKNWVEVPWKAASKSTHCYACQIPFPHPPAKPEELSAPAKTATGTSVSSRYACTICHNHFCIDCDVFCHETLYNCPACQSSVPKGRISNGARGRSDPMEVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKA9</accession>
    <name>D5GKA9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00009453001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Transcription factor involved in RNA polymerase II transcription regulation. May function in both SPT15/TBP post-recruitment and recruitment steps of transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IWS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430337">
        <property type="protein sequence ID" value="CAZ84952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840761.1">
        <property type="nucleotide sequence ID" value="XM_002840715.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKA9"/>
    <dbReference type="FunCoup" id="D5GKA9">
        <property type="interactions" value="334"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GKA9"/>
    <dbReference type="GeneID" id="9181972"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009453001"/>
    <dbReference type="eggNOG" id="KOG1793">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045275_1_0_1"/>
    <dbReference type="InParanoid" id="D5GKA9"/>
    <dbReference type="OMA" id="MPAYNIQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.10">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051037">
        <property type="entry name" value="RNAPII_TF_IWS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035441">
        <property type="entry name" value="TFIIS/LEDGF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017923">
        <property type="entry name" value="TFIIS_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46010">
        <property type="entry name" value="PROTEIN IWS1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46010:SF1">
        <property type="entry name" value="PROTEIN IWS1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08711">
        <property type="entry name" value="Med26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47676">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51319">
        <property type="entry name" value="TFIIS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TFIIS N-terminal" evidence="5">
        <location>
            <begin position="213"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="81"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="305"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="91"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="101"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="311"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00037349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00037992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ84952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="395" mass="44909" checksum="2E5FDFC63D17D316" modified="2010-06-15" version="1">MSELELLEHRGSPPPNALTEENLNTLSPAHEEASKASDNESVLSDIDEGIFQEFDDSLIGRIVPIDEETVHTIGKYKKKLDPNQLAEKSGKKEKRRRDIKRRRDDVEERTGESEPQEQELTAEEKARRELDQRMDAALKGPKKKKKKGEDLEEMNDDLIIALKQRMIDAANRDQEAIVNGQQAVHKLAMLEEVKDILQRPNLMATAMDNNLLEAMKRWLEPLPNKALPAYGIQKVMFEIMGKMKITTDYLRESGIGKVVVFYTKDKRPQLHIKREANRLVRDWSRPILGRSDDYHSREIPMAHNGLVYPTRDPHSSQRGDNEEANPLAPPDRNKNRTRVPQAMPRSYEIAPASRVVQGHNAYARPIGAAGDDIIRRMKAKKQAANKGKKSGMIIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKP6</accession>
    <name>D5GKP6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00009687001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430340">
        <property type="protein sequence ID" value="CAZ85089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840898.1">
        <property type="nucleotide sequence ID" value="XM_002840852.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKP6"/>
    <dbReference type="GeneID" id="9184751"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009687001"/>
    <dbReference type="HOGENOM" id="CLU_2998157_0_0_1"/>
    <dbReference type="InParanoid" id="D5GKP6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ85089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="57" mass="6185" checksum="19322F1ECCABB342" modified="2010-06-15" version="1">MAMVEDVGRGERRKGGVLTLILPVRYSPAVLVRLLRVIAAVYYTSPGGCARLIRTGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GL43</accession>
    <name>D5GL43_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00009985001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Regulates mitochondrial small subunit maturation by controlling 15S rRNA 5'-end processing. Localizes to the 5' precursor of the 15S rRNA in a position that is subsequently occupied by mS47 in the mature yeast mtSSU. Uses structure and sequence-specific RNA recognition, binding to a single-stranded region of the precursor and specifically recognizing bases -6 to -1. The exchange of Ccm1 for mS47 is coupled to the irreversible removal of precursor rRNA that is accompanied by conformational changes of the mitoribosomal proteins uS5m and mS26. These conformational changes signal completion of 5'-end rRNA processing through protection of the mature 5'-end of the 15S rRNA and stabilization of mS47. The removal of the 5' precursor together with the dissociation of Ccm1 may be catalyzed by the 5'-3' exoribonuclease Pet127. Involved in the specific removal of group I introns in mitochondrial encoded transcripts.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Binds to mitochondrial small subunit 15S rRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCM1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430346">
        <property type="protein sequence ID" value="CAZ85236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841045.1">
        <property type="nucleotide sequence ID" value="XM_002840999.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GL43"/>
    <dbReference type="STRING" id="656061.D5GL43"/>
    <dbReference type="GeneID" id="9182074"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009985001"/>
    <dbReference type="eggNOG" id="KOG4197">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002863_0_0_1"/>
    <dbReference type="InParanoid" id="D5GL43"/>
    <dbReference type="OMA" id="PQACLYQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000217">
        <property type="entry name" value="Pentatricopeptide repeat domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47447">
        <property type="entry name" value="OS03G0856100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47447:SF17">
        <property type="entry name" value="OS12G0638900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13812">
        <property type="entry name" value="PPR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="5">
        <location>
            <begin position="207"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="5">
        <location>
            <begin position="351"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="5">
        <location>
            <begin position="955"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="5">
        <location>
            <begin position="990"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="132"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00044493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00044511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ85236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1262" mass="139879" checksum="B5D62D61B60AAC22" modified="2010-06-15" version="1">MVFKPFTTLARQSISKHLVNGCAQSVVAATQSSYASSTVQLSRLGQQNAPARLHTAFGGANSGRAAPGKDGQGSDSLGAYYAAQSQAVDEKEEKENRKYLFSRKILWSKAQHQQQQKALLENPPSLELGSPRSRSSSITQVEAQLPSKDIVKGEEEEAVFVESVAEDSEILSPEEVAPVEDAKAEFAPSPTPSEETLVPASPEVSGVTAQYNDQLRDLRTAGKYEEILALFEHMIQEGVQPSTTTYNHVLVALIQTGAGGIPRVLSVYQDMLRNKVLPSTVTYSILIDYLAASGKSSLQFARRIEDDILRFGFVVPSRRRELDEIRSQKSLDMALDIFYASTEVRVERTFSEPVYSILIEACAENGREEDMLKVYTHMELHDVLPSLDTTLSLIKGFGKAENICSAVETYNNYCALDTSKKPANVDERYLVYKALILAYMEAGDPAGALSFLEKIVGTSKDPHRSQWLAEAIIKGFLQRSDLGSAKEWIAGRMAGTSEYIGLSRVLIGVADLGDYGLADRLYSEFMDYVRLHSINHSSTSIAQMAFLNLCVKERKIGRARAVWNDLSERVLSSGPDVEAAVVYSMMLFENRLTNEALVVLKQFSEFFMEKPLGVPLALKAEYLQEAFESLIDRLAAKDLLTHGVALDISGFSMRHCGRMGAPASRIVLGLFGEQRILGLTLPQLDLLLQIQAGVFQQPGELVSGDMEKFEQMLTLALNAGVPLSKAMKISVDGVIAARGIDEGLLAKWTAFVQSQKVWENMPMAPLTEDVVASPTLVPSEGETAVAEDNHDPYWAKTDVKVSQAIDNALEGTKAARARLMDARRMYQQTRKAGRLVRMTTLARMVGAASRAGQPEFMEEIYRNARMDTPLLMEFRSVRYVWCVLLDAMVAGQLSIGNRQKAAVYHEEMLKMRASPSANTYGLYIVSLKGSNQTYDEASEALKIFERAKAENVLPSSFLYNALIGKLAKARRVDDCLFYFAEMRTLGIKPTSVTYGTMINALTRVGDEHFAEELFQEMEAMPNYKPRPAPYNSLMQFFVNTKRDRSKVMSYFNRMTSLGIEATSHTYKLLIEAYATLDPPDMPAAEGVLDLIRAKNQPVESSHHAALIHAKGCVLQDVEAAISHFKSLLHPTTATPQPQLDNTIYQALFESLVANHRVQDTTLWLSDMGRRGVRMTPYIANTLIHGWALEKDIGKAEEVYESLSKDENARLKREPSTYEAMTRAYLAVEDRAGALRVVDEMSERGYPSAVMVRVADLVRGRGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>D5GL47</accession>
    <name>D5GL47_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">Phosphomannomutase</fullName>
            <ecNumber evidence="5 13">5.4.2.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00009989001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>alpha-D-mannose 1-phosphate = D-mannose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:11140"/>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
            <dbReference type="ChEBI" id="CHEBI:58735"/>
            <dbReference type="EC" id="5.4.2.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 13">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the eukaryotic PMM family.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.8" evidence="5 13"/>
    <dbReference type="EMBL" id="FN430346">
        <property type="protein sequence ID" value="CAZ85240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841049.1">
        <property type="nucleotide sequence ID" value="XM_002841003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GL47"/>
    <dbReference type="FunCoup" id="D5GL47">
        <property type="interactions" value="684"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GL47"/>
    <dbReference type="GeneID" id="9185834"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009989001"/>
    <dbReference type="eggNOG" id="KOG3189">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065642_0_1_1"/>
    <dbReference type="InParanoid" id="D5GL47"/>
    <dbReference type="OMA" id="ISHRVYT"/>
    <dbReference type="UniPathway" id="UPA00126">
        <property type="reaction ID" value="UER00424"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004615">
        <property type="term" value="F:phosphomannomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009298">
        <property type="term" value="P:GDP-mannose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006013">
        <property type="term" value="P:mannose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02585">
        <property type="entry name" value="HAD_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1240.20:FF:000001">
        <property type="entry name" value="Phosphomannomutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1240.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005002">
        <property type="entry name" value="PMM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043169">
        <property type="entry name" value="PMM_cap"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10466">
        <property type="entry name" value="PHOSPHOMANNOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10466:SF0">
        <property type="entry name" value="PHOSPHOMANNOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03332">
        <property type="entry name" value="PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00445">
        <property type="entry name" value="alpha-phosphomannomutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01143">
        <property type="entry name" value="C2.B.3:_Phosphomannomutase_Lik"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01140">
        <property type="entry name" value="C2.B:_Phosphomannomutase_and_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="10">
        <location>
            <position position="24"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="10">
        <location>
            <position position="26"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR605002-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR605002-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR605002-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ85240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="263" mass="29701" checksum="ADCF54F108C90C9D" modified="2010-06-15" version="1">MSAAASQFPPLTQRPIANTICLFDVDGTLTPARRTVSPEMLALLQKLRAKVAIGFVGGSDLVKQQEQLGVNGLNVIDIFDYCFAENGLTAYKLGEALPSNSFITWLGEEKYKKIANFILHYIADLDIPLKRGTFIEFRNGMINVSPIGRNASIQERHDFEAYDKIHNVRRDFVEALKMNFADYGLTYSIGGQISFDVFPTGWDKTYCLQHVEKEGVFSQIHFFGDKTYKGGNDYEIYEDSRTIGHSVTKPEDTIKMLKELFDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>D5GL59</accession>
    <name>D5GL59_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00010019001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430346">
        <property type="protein sequence ID" value="CAZ85252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841061.1">
        <property type="nucleotide sequence ID" value="XM_002841015.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GL59"/>
    <dbReference type="STRING" id="656061.D5GL59"/>
    <dbReference type="GeneID" id="9182046"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010019001"/>
    <dbReference type="eggNOG" id="ENOG502S95Z">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055856_0_0_1"/>
    <dbReference type="InParanoid" id="D5GL59"/>
    <dbReference type="OMA" id="HESTPQQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040015">
        <property type="entry name" value="UBL3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039540">
        <property type="entry name" value="UBL3-like_ubiquitin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13169:SF0">
        <property type="entry name" value="UBIQUITIN-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13169">
        <property type="entry name" value="UBIQUITIN-LIKE PROTEIN 3 HCG-1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13881">
        <property type="entry name" value="Rad60-SLD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBL3-like ubiquitin" evidence="2">
        <location>
            <begin position="168"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="231"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="92"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ85252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="267" mass="29179" checksum="CBC4C10A10207473" modified="2010-06-15" version="1">MSAKTSTQPAQDEPALDVPDTRTHDETPTSLSLGTTEINHTSQEHLAPGQPPFATPITSNPSQSTEGPNVRSPSPPMLPIVALEPTLSPVNSAATSSPGSTTDDSTPPPPPSEMKPPSSSLPITASKPSLSITLLLISGVRHQFVINESYLESHSVTGGTGKDSLKDPFEMSVWQLKECIWKDWRDEWDQRPASALFVRLIHFGRMLDDKQHLKDCKLSHDSPNVVHMTIRPAETGDDEMTQRSTKGGFGSRQRDDQRSPRCRCVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5GL85</accession>
    <name>D5GL85_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00010057001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430346">
        <property type="protein sequence ID" value="CAZ85278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841087.1">
        <property type="nucleotide sequence ID" value="XM_002841041.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GL85"/>
    <dbReference type="GeneID" id="9182072"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010057001"/>
    <dbReference type="HOGENOM" id="CLU_2689600_0_0_1"/>
    <dbReference type="InParanoid" id="D5GL85"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="74" mass="8345" checksum="4432CEC5EC420EC5" modified="2010-06-15" version="1">MKERKINTSHYSLLNPILITPLIPLGSLTAPHSQSCAADNSSGNRDRNRTRTRINRGRDRGRYLLLGSYMFPCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLK6</accession>
    <name>D5GLK6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="12">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00010250001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430349">
        <property type="protein sequence ID" value="CAZ85399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841208.1">
        <property type="nucleotide sequence ID" value="XM_002841162.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLK6"/>
    <dbReference type="FunCoup" id="D5GLK6">
        <property type="interactions" value="333"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GLK6"/>
    <dbReference type="GeneID" id="9182702"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010250001"/>
    <dbReference type="eggNOG" id="KOG0756">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015166_5_0_1"/>
    <dbReference type="InParanoid" id="D5GLK6"/>
    <dbReference type="OMA" id="KDWYKGG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005469">
        <property type="term" value="F:succinate:fumarate antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000021">
        <property type="entry name" value="SFC1p Mitochondrial succinate-fumarate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049563">
        <property type="entry name" value="TXTP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45788">
        <property type="entry name" value="SUCCINATE/FUMARATE MITOCHONDRIAL TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45788:SF2">
        <property type="entry name" value="SUCCINATE_FUMARATE MITOCHONDRIAL TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="16"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="119"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="221"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ85399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="326" mass="35808" checksum="14B34E73B6D9AAD4" modified="2010-06-15" version="1">MSSKSGNGNGNVKKKPTAAVHLIAGGGAGLCEALACHPLDTIKVRMQLSRRNRAPGVKRKGFFTVGKEIVKRETPLGLYKGLGAVVTGIVPKMAIRFSSFEFYKSLAKVHPGTGNISAKAVFISGLAAGATEAVAVVTPMEVIKIRLQAQHHSMADPLDIPKYRNAAHAAYTVVREEGFRTLYRGVTLTALRQSTNQAVNFTAYTYLKQYALRIQPNISELPSYQHLLLGLVSGAMGPLSNAPIDTIKTRLQRSEARPGESALRRITFIAKDMFKHEGFRAFYQGITPRIMRVAPGQAVTFMVYEFIRGHIERFQVSGDNDNQYRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLP4</accession>
    <name>D5GLP4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00010299001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430350">
        <property type="protein sequence ID" value="CAZ85437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841246.1">
        <property type="nucleotide sequence ID" value="XM_002841200.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLP4"/>
    <dbReference type="STRING" id="656061.D5GLP4"/>
    <dbReference type="GeneID" id="9182740"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010299001"/>
    <dbReference type="eggNOG" id="ENOG502SJN2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_507337_0_0_1"/>
    <dbReference type="InParanoid" id="D5GLP4"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="391"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="435"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="435"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="537" mass="57210" checksum="4573E040F1305DA8" modified="2010-06-15" version="1">MTTEAVRMLSYTLPCPLPTASAYSATPTPIPSNLGSNPDRLPASAAAAPAVAAAAGQQFSPAITARCHSRAQADSGSSTPNGLIGADDGITTALHTLTRNIQRRCSSDGTKRVYIHVSHAISPGISLNRLPSTPPANGCVGSPGGFFGGGFDTSPGGCADGGGGYFAPTVFNSIVVAPENVPTAPSMSFHRTHTIPPPNPILPPFSLHMTLLERYIPPTSNAEDISMFSTQSSVILDRLHELSPRGGSLLFIYPTKTGAKHFDKQYLGPVLDPLLRKLMVLYMLREDLLWGIRNMAAIEKMNEFEGLRWKLDNFCTRFSHAINDNSMWGKSGSGSQASRLQLDSSYPKTRVRLVHSQKAMIQLNDNSWREWWSQQEQLRIREAVKRHFSSLQPPAEGQTPVSPNASGPHGSHHAKSASMSSPKLGSFPSLAIKVTTASSTPSSPTGPSSPQSKYSFGYGAPGDLAREVLDGVRAPAVRPSSRGMSEAVVASALAGPGTFGVGGVVGDYEISSVPSDRRVKERGIEVGVFVLRREIVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLU4</accession>
    <name>D5GLU4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00010423001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430351">
        <property type="protein sequence ID" value="CAZ85511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841320.1">
        <property type="nucleotide sequence ID" value="XM_002841274.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLU4"/>
    <dbReference type="STRING" id="656061.D5GLU4"/>
    <dbReference type="GeneID" id="9186495"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010423001"/>
    <dbReference type="eggNOG" id="ENOG502SHYC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_377759_0_0_1"/>
    <dbReference type="InParanoid" id="D5GLU4"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055936">
        <property type="entry name" value="DUF7514"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39611:SF2">
        <property type="entry name" value="HYDROXYPROLINE-RICH GLYCOPROTEIN DZ-HRGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39611">
        <property type="entry name" value="HYDROXYPROLINE-RICH GLYCOPROTEIN DZ-HRGP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24355">
        <property type="entry name" value="DUF7514"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7514" evidence="2">
        <location>
            <begin position="224"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="199"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="401"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="470"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="561"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="47"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="78"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="203"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="432"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="482"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="567"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="592"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="662"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="686"/>
            <end position="702"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ85511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="734" mass="81648" checksum="5BB9AA42930F5151" modified="2010-06-15" version="1">MLTSRSSRLGQGHGRFLPGPVSGSGHGPGYHHSHGGGGGGPPNFFASYSNPYRNSSSYPHPRTPSSPTHTTRSKSRSHSSSSSQPTSPCLDNFSTTACLGSVAEDEEVPVSGLHHHHHHPSHTSTRLKDHHHPAPSPPLRLACTTSKRAPPSLPFLRTDTNLDDFVLTPIASTPEPLNSWFERRKFEELELIQRRRFRPKMSPPQQQQQQQQPPSVDPGQYWGYLISSDHPTQPTPLFRNLLLSIANYIIDCLEPRDIHCLTPEKISGFYRLVGGDLDELFLGTPAEKLSGIYTTLGCSHTLQPIPDDDFVPPSIPALTPRGFAIWQTIQLLLDPEEHVPYLQEAVRKFQLANPETGELFPKSIPNGAFPSKPDEETVKWHDDMFEKKSLADSAAAAAAAAMPVPGAVPPEEPVWNGYHHGNSHQQHTSSSKSKRKSRKSGSRHRATVEEVVDEGLYARVPPIISPADSYTTHHYGHHAHTHHVEPFNSDTEHHHGSRNHSRRGREYPVSYVSQNGLVTPVVPVYPNGTATLIPEAIHMQPEELSPTLPYGRAARYASTPMTVDPTSSCSSSYSDLHNSNSSPYHHSSTSSPHHHSSSRHHTRRSPSPLYREPHLRKSASAYCYTSRPVAPVPPSRSRSGGSSRGRRRSHMISDDSDSAEEWVIKGERMREDDEEERRRRKSDHARRRELERERERVREGGRSDTWGGYGGKSMSGGGRYADSHYLHTGSGVFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>D5GM29</accession>
    <name>D5GM29_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">MICOS complex subunit MIC12</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">Altered inheritance of mitochondria protein 5, mitochondrial</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9 11">Found in mitochondrial proteome protein 51</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00010498001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the mitochondrial contact site and cristae organizing system (MICOS) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Mitochondrion inner membrane</location>
            <topology evidence="11">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the MICOS complex subunit Mic12 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430351">
        <property type="protein sequence ID" value="CAZ85572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841381.1">
        <property type="nucleotide sequence ID" value="XM_002841335.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GM29"/>
    <dbReference type="GeneID" id="9187356"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010498001"/>
    <dbReference type="eggNOG" id="ENOG502SC1K">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134520_2_0_1"/>
    <dbReference type="InParanoid" id="D5GM29"/>
    <dbReference type="OMA" id="WNAELEN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061617">
        <property type="term" value="C:MICOS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044284">
        <property type="term" value="C:mitochondrial crista junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042407">
        <property type="term" value="P:cristae formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031463">
        <property type="entry name" value="Mic12"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17050">
        <property type="entry name" value="AIM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ85572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="109" mass="12238" checksum="B7DDAA330A2F3819" modified="2010-06-15" version="1">MGIIGGFLGGTILTGSIAYLALRHLNANTRAHSTSLRESRQIADSILLPKQVPKNRDPTLERPSFVETLKDTWNWEIEKIVRRVQGWDLRSTREGVEDTIGNVVEKLKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>D5GME8</accession>
    <name>D5GME8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00010675001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the DEAD box helicase family. DEAH subfamily. DDX15/PRP43 sub-subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="FN430354">
        <property type="protein sequence ID" value="CAZ85691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841500.1">
        <property type="nucleotide sequence ID" value="XM_002841454.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GME8"/>
    <dbReference type="FunCoup" id="D5GME8">
        <property type="interactions" value="1408"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GME8"/>
    <dbReference type="GeneID" id="9182117"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010675001"/>
    <dbReference type="eggNOG" id="KOG0925">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001832_5_11_1"/>
    <dbReference type="InParanoid" id="D5GME8"/>
    <dbReference type="OMA" id="MKVYPLY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005684">
        <property type="term" value="C:U2-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000390">
        <property type="term" value="P:spliceosomal complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17973">
        <property type="entry name" value="DEXHc_DHX15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000007">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000003">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000578">
        <property type="entry name" value="probable ATP-dependent RNA helicase DHX35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044756">
        <property type="entry name" value="DHX15_DEXHc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF109">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX15 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="127"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="300"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00055599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ85691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="761" mass="86787" checksum="19D3B0EB41802ADB" modified="2010-06-15" version="1">MSTTEDRKRKKPQEKESQTNSPKRHKSEPVSPPSGNTKKPEIDPSKNPYLAHTYPAEENKSDWGPLAGFKRHRTTTKMANIAEDGPFNPFSGNKLSDRYFNILEGRRELPIAAQRDEFLNMFHKSQTMTTQIPQYVLFDDLPHLNGYKQVACTQPRRVAAMSVAKRVADEMDVELGHEVGYSIRFEDKTSPNTILKYMTDGMLLREAMHDHLLERYSCIILDEAHERTLATDILMGLLKNVVERRPDLKLIIMSATLDAQKFQHYFNDAPLLAVPGRTHPVEIYYTPEPERDYLEAAIRTVLQIHTTEPEGDILLFLTGEEEIEDACRKINAEVDLLVREADVGPLKSYALYGTLPPQQQQRIFEPAPSPFKPGGPPGRKVVVSTNIAETSLTIDGIVYVVDPGFSKQKVYNPRIRVESLLVSPISKASAQQRAGRAGRTRPGKCFRLYTESAFKKELIDQTYPEILRSNLANTVLELKKLGIDDLVHFDFMDPPAPETMMRALEELNYLACLDDDGNLTTLGKLAAEFPLDPALAVMLISSPEFFCSNEILSLTALLSVPQLFVRPLNNRQRADEMKKLFAHEDGDHLTMLNVYHAFKQQDSQPAWCREHFLSYRALQSADNVRAQLQRIMERLEIPLVSTDFSEKSYYENIRRALVSGFFMQVAMKNSPGKAYITVKDNQDVLIHPSTALEKDSPWLVYNEFVLTTKSFIRTVTHIRPEWLLEIAPGYYDVKDFREGPVRTGLERTMDKVRKRNALKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMF0</accession>
    <name>D5GMF0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Aspartate-semialdehyde dehydrogenase</fullName>
            <ecNumber evidence="4">1.2.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">GSTUM_00010677001</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the NADPH-dependent formation of L-aspartate 4-semialdehyde (L-ASA) by the reductive dephosphorylation of 4-phospho-L-aspartate. Mediates the second step in the biosynthesis of amino acids that derive from aspartate (the aspartate family of amino acids), including methioinine and threonine, the latter of which is a precursor to isoleucine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-aspartate 4-semialdehyde + phosphate + NADP(+) = 4-phospho-L-aspartate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24284"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57535"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="1.2.1.11"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="11">
            <dbReference type="Rhea" id="RHEA:24286"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 2/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homotetramer; dimer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the aspartate-semialdehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.11" evidence="4"/>
    <dbReference type="EMBL" id="FN430354">
        <property type="protein sequence ID" value="CAZ85693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841502.1">
        <property type="nucleotide sequence ID" value="XM_002841456.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMF0"/>
    <dbReference type="FunCoup" id="D5GMF0">
        <property type="interactions" value="322"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GMF0"/>
    <dbReference type="GeneID" id="9182119"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010677001"/>
    <dbReference type="eggNOG" id="KOG4777">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049966_1_0_1"/>
    <dbReference type="InParanoid" id="D5GMF0"/>
    <dbReference type="OMA" id="CEEEMKM"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00016"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00463"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00464"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004073">
        <property type="term" value="F:aspartate-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071266">
        <property type="term" value="P:'de novo' L-methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009090">
        <property type="term" value="P:homoserine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd18130">
        <property type="entry name" value="ASADH_C_arch_fung_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02315">
        <property type="entry name" value="ScASADH_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000200">
        <property type="entry name" value="Aspartate-semialdehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000016">
        <property type="entry name" value="Probable aspartate-semialdehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051823">
        <property type="entry name" value="ASADH-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000319">
        <property type="entry name" value="Asp-semialdehyde_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005676">
        <property type="entry name" value="Asp_semi-ald_DH_pep-lack"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000534">
        <property type="entry name" value="Semialdehyde_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012280">
        <property type="entry name" value="Semialdhyde_DH_dimer_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00978">
        <property type="entry name" value="asd_EA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006416">
        <property type="entry name" value="PRK08664.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46718">
        <property type="entry name" value="ASPARTATE-SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46718:SF1">
        <property type="entry name" value="ASPARTATE-SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01118">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02774">
        <property type="entry name" value="Semialdhyde_dhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000148">
        <property type="entry name" value="ASA_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00859">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01103">
        <property type="entry name" value="ASD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="domain" description="Semialdehyde dehydrogenase NAD-binding" evidence="15">
        <location>
            <begin position="9"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="14">
        <location>
            <position position="155"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00050041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000148-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAZ85693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="361" mass="38933" checksum="4CD3182CC35FEFE9" modified="2010-06-15" version="1">MSSSVPAKKAGVLGCTGSVGQRFILLLSQHPYLALHAVGASSRSAGKKYSEATKWKQDQLMPNRVRDMIVKDCKAAEFRDCDVVFSGLDAEVAGEIEMEFIKNDLVVFSNSKNYRRDPITPLIVPLVNTSHFSIIPHQRSQNYLKKGFLVTNANCSTTGLVVPLKALQDAFGGIEFVTVTTLQAISGGGYPGVPSMDILDNTVPLIGGEEEKIEWETGKILGSVNPTSSGFDMLSTIKVSAQCNRVAVMDGHTACVSLRFAKRPPPSIEQVKAALRDYVSEPQQLGCPSAPRNAIVVMEEEDRPQPRLDRNTDGGYAVSVGRIRPDNIFDIKFVTLSHNTVIGAAGSSILNAEAAVVKGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMG5</accession>
    <name>D5GMG5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00010694001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430354">
        <property type="protein sequence ID" value="CAZ85708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841517.1">
        <property type="nucleotide sequence ID" value="XM_002841471.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMG5"/>
    <dbReference type="GeneID" id="9182134"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010694001"/>
    <dbReference type="eggNOG" id="ENOG502S00W">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_337706_0_0_1"/>
    <dbReference type="InParanoid" id="D5GMG5"/>
    <dbReference type="OMA" id="WIVNASE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072150" evidence="3">
        <location>
            <begin position="25"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="199"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="235"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="317"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="345"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="688"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="696"/>
            <end position="708"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ85708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="764" mass="84132" checksum="8334B5361AE469E8" modified="2010-06-15" version="1">MGSTTSTLRASTIILLALTPQALAADGADFSNNLFSDLAPVLALFGEQVSKQFLSQSLTWLEDVIFAMAPLGIVTAIVSAVRVGGPNWLKAIVGRARESRAVAEVELMSSTSHEVCELWNGEAIVRVMGSPEVAEILYFEDETDGQGGPRFLTLEAAKVAGILENVEELKSDPNLQGDASISAPNISLNICEANEQGELWLFAILGIVLQFGCLIFIGITRYHPTINWKDQNSEYAYPLMASGTLLVVMGMMACSRAVQASSEEDIWVSKRSFRILWVQKSSRVNDQHFGSYALFGDGERKKLITSRRAYKYSSRNLLRTLSIVGSFVCIAGFVTQFVGLRALHWSASVAQLVVTLAMTAARTWIRRKMSERPRAMTLPVDKDGLDGLTLTFGTSPDKLWGAENSSIRQNSNSNWEVITPHKYLGYEDAQEAHNAPYKDAQNIIRMRMELEHSTSWKGSSHSFVQELDACLRSLLACLMGSKDITLVDSFERSEVFRWAMPVKVDRVVQCITMTAEKSYPSTWIVNASELRAVISLWCYGENPSRIKNPYRLPCLGDDPRAVLRVLGPATHRFIRDCERWVDRGGLFAVVENGKLRGNTNQVRVTAQVLSETIARMRGHTDDTTGGVDMYFILCDHVLGTEPVDSDGCNNSLTLVSTVPLRQMCAQEILSSFLWAIVGTVLSINGDTQSRSATPLGDHESMNDERPGSKLENSFFRELAAIITNSGVTSNIQDAYALIIPPFAAAGKLPRGPSSELAQDEIESI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMQ7</accession>
    <name>D5GMQ7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00010888001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SEN54 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430359">
        <property type="protein sequence ID" value="CAZ85800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841609.1">
        <property type="nucleotide sequence ID" value="XM_002841563.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMQ7"/>
    <dbReference type="FunCoup" id="D5GMQ7">
        <property type="interactions" value="52"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GMQ7"/>
    <dbReference type="GeneID" id="9185136"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010888001"/>
    <dbReference type="eggNOG" id="KOG4772">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028449_0_1_1"/>
    <dbReference type="InParanoid" id="D5GMQ7"/>
    <dbReference type="OMA" id="MYMRLRH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032473">
        <property type="term" value="C:cytoplasmic side of mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000214">
        <property type="term" value="C:tRNA-intron endonuclease complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000379">
        <property type="term" value="P:tRNA-type intron splice site recognition and cleavage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024337">
        <property type="entry name" value="tRNA_splic_suSen54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024336">
        <property type="entry name" value="tRNA_splic_suSen54_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21027">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21027:SF1">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12928">
        <property type="entry name" value="tRNA_int_end_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA-splicing endonuclease subunit Sen54 N-terminal" evidence="4">
        <location>
            <begin position="70"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ85800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="395" mass="45023" checksum="42DC91BA42ABEA26" modified="2010-06-15" version="1">MDEPEDGNIRNAQNNIQDADLSDETQDFRFLNLLNKTGTTALPRRGEKDFEPDGTDLQDRVLNESRQAMHEALLGERKHSSKQHVSATWRPSQRMAEVHVARGPHFKTVGKADRRGVLWLLPEETIYLVERGNLECWWEEGVPMSLQGVYASALDGCGGLERYQVYTYLKRAGYILARAPTFFHDDDKVVEREERGEGIIGTIRPATTTPPGIFKQLFTSLFLTRTKPPPFGPVIAQGTYRTYDSIYSRLSIIPTHHPPHTPTNMVAVASETPFRIAYKVWKPQPHFRKSDPPPADFLVAVASARETSLPTLSQLSALFDSVPVDEKTGGKSQFRRLKDGHRKCLVSQSWDTEVTTFIKFAHVGFWVGPIFYGKREGEGWREIGRWCRRERTRQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMR1</accession>
    <name>D5GMR1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00010892001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430359">
        <property type="protein sequence ID" value="CAZ85804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841613.1">
        <property type="nucleotide sequence ID" value="XM_002841567.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMR1"/>
    <dbReference type="FunCoup" id="D5GMR1">
        <property type="interactions" value="930"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GMR1"/>
    <dbReference type="GeneID" id="9185140"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010892001"/>
    <dbReference type="eggNOG" id="KOG1426">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005210_4_0_1"/>
    <dbReference type="InParanoid" id="D5GMR1"/>
    <dbReference type="OMA" id="IFVWGTG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051553">
        <property type="entry name" value="Ran_GTPase-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982:SF1">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00625">
        <property type="entry name" value="RCC1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="38"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="RCC1-like" evidence="5">
        <location>
            <begin position="40"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="95"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="171"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="224"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="279"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="338"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="402"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="113"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="122"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ85804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="465" mass="50064" checksum="7EB8BB85C6A03ED9" modified="2010-06-15" version="1">MVEEAEREKSVEPARKKGRAKAKALADPKITSVPTRRLNIYAFGTGDNAELGLGPEVNAKVVKRPRLNTHLLPEKVGIVAVAIGGMHGLALSHEGKVYSWGVNDQYALGRETKYTPPVGDVGSDEDSDSDDEEPLNPLESMPMLITAFPEGTVITNIAAGDSVSIAVTDTGKVYGWGTFRCSDGILGFNEKTRIQSVPVLLPTLKNIVQVSVGTDHVLGLTREGNVFAWGNGQQFQLGRRVVERTRLNGLIPREFGLPRHQVRYVATGSYHSFAITEDGKVWAWGLNQFGQCGIYDPKHAGEDNTVVPVPTVVQGLIEHKIVQISAGEHHSAAVTEDGELLVWGRLDGGQLGLDPETLPAEDVVRDVSGKPRYLSTPRAVPDIKFSFIGCGTHHNIAISREGQAYSWGFGGSYQTGLGPEADDEIKTPTKIENTATRGVKMVFSDAGGQFSILAGIPPKLTNGTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMY0</accession>
    <name>D5GMY0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00011017001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430361">
        <property type="protein sequence ID" value="CAZ85893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841702.1">
        <property type="nucleotide sequence ID" value="XM_002841656.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMY0"/>
    <dbReference type="STRING" id="656061.D5GMY0"/>
    <dbReference type="GeneID" id="9183461"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011017001"/>
    <dbReference type="eggNOG" id="KOG1840">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_300380_0_0_1"/>
    <dbReference type="InParanoid" id="D5GMY0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025676">
        <property type="entry name" value="Clr5_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053137">
        <property type="entry name" value="NLR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46082:SF6">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46082">
        <property type="entry name" value="ATP/GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14420">
        <property type="entry name" value="Clr5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13374">
        <property type="entry name" value="TPR_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Clr5" evidence="2">
        <location>
            <begin position="20"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="196"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="434"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="196"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="375"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="501"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ85893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="997" mass="107639" checksum="ED301718FE91287D" modified="2010-06-15" version="1">MSDSMSWESRFRTREIAACEWEGIRGVFEDLYIEQGQTLKMIRQHLAEEYGFYANETQYKKKIRKWDIQKNLSRDKMVAIVRLLDHRTAQGLCTKFSFNGKPLSHERIDRSRKRFNMLGPDAPIEGEIDYEIEGVEYWDPKADPLASQPDAGFPAQPELQASGQPAPVDMQKTQSPQLPEMRVPAHDGVVYVAQTEIPTTRNPEQNGNDGAAYQLPNDDECYAKPSSKPTLVNVGGLKQASKRRRSDSDADDTVDNRKSRRTSRHGDNGDPSDALAQPANLIDKDDGEENGSSSMPTPTPIPMERRRSLSSTSGEAGSPGLGFQVPSIRSAGSPSPEPLPSGPILGGGLPPLPPPERRDSNISLPSFSELEAYSPPQSLPLQAGLSSGTLPPPLVQWGTSPPSPAVVWGPGQPTQRKSSILALALGESPAPILNDIYWPEPPSAGDFSGGDTVQGPGPAEVLSEPITRNPEDGPLSCTSRESYEGAPRNEYIPGATNAGGSSLRSSSPPSLMSLTSVLSSPASSHHSPSLSQPLRHNSPSPEPPATPFQATISQEITEWVAAAIRYSTKSWWNDLRDLEVQEVQNLINRLGRKHLDTLDAVSALATLYTTTGKDTRAMGLHKYVYDQLHKFFGSTHPLTVRAQHNLAGVYLAKGDYPAALAMAKESVEHSKIVHGADHAETLSSLVQLGHVLSRLGELDKAMQIANSVLEAQQRKPEGVANRHTTISANKLLGTLLEKKGCYQPAKAILEEVLALQEELFGKDHPETLSVMDCLGTTYLSLGEPAKSEEILRRTVDLSTEKLGGKHPDTLSSKHNLVNALLKSRDFAAAKDLAGTVYAETRQVLGDEHVDTAFSMVNQASALMGIGDVAGAVNILRATVAKLAVILPAGNSHVLLVKEKLAQGLVDSFRFPEAERVMREVLVERRCALGDAHPETIRVEEWLGMCRARMGGAEAVAGSGESAATATAGEWTGKGGVSRGGCRNGGIRARPSWWCGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>D5GN80</accession>
    <name>D5GN80_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00011142001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430362">
        <property type="protein sequence ID" value="CAZ85973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841782.1">
        <property type="nucleotide sequence ID" value="XM_002841736.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GN80"/>
    <dbReference type="GeneID" id="9185899"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011142001"/>
    <dbReference type="HOGENOM" id="CLU_1023752_0_0_1"/>
    <dbReference type="InParanoid" id="D5GN80"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="171"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="224"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="150"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ85973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="272" mass="28407" checksum="428939E7A3C61A0B" modified="2010-06-15" version="1">MGAIVTPPPHPDLVKRATANFYCTGVMADVQCPSVRGFKGNCVVVSSTGQCCYETNIARCARGGDCRDFTAGCGTACCTEAFPYCNTEVVPNDAVYPHCEQTSNPTYTNSYYLNGNTPGIGVLTSRRSDLGLTGTTKPGSSPSIVTVTHSTAPPSATTSRGTDSEPMSGGAIAGIAIGGAAGLGALALGVFFLFFKKKPTAPAQSGGMAEYTQQQHLQQYVPQPIQSPGFAHGHDDPSTPKPYPEQQLAEAPANETPIVGYRPAWELPASNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNC7</accession>
    <name>D5GNC7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00011224001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430363">
        <property type="protein sequence ID" value="CAZ86020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841829.1">
        <property type="nucleotide sequence ID" value="XM_002841783.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNC7"/>
    <dbReference type="STRING" id="656061.D5GNC7"/>
    <dbReference type="GeneID" id="9182220"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011224001"/>
    <dbReference type="eggNOG" id="ENOG502QVPZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_816815_0_0_1"/>
    <dbReference type="InParanoid" id="D5GNC7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050358">
        <property type="entry name" value="RSE1/DDB1/CFT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004871">
        <property type="entry name" value="RSE1/DDB1/CPSF1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10644">
        <property type="entry name" value="DNA REPAIR/RNA PROCESSING CPSF FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03178">
        <property type="entry name" value="CPSF_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RSE1/DDB1/CPSF1 C-terminal" evidence="1">
        <location>
            <begin position="7"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ86020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="340" mass="37798" checksum="E92783CEE8A8F2C5" modified="2010-06-15" version="1">METEGKIYRYIVVGTSYINVTASSLLKGRVLVLSVILGPSGGVEVRRRYQIQCDAPVYSLAPYSSSSLVYCSGHSINMRTLNAQTKKFDVVAEKKLRSPAVQITVAEPWIHLSCGEDSMLVLKFEDDKFNELFSDEVARNGFHHFQLLQGFFLATDKELGVVGLWQPPDRRNVESLVTIIEAELTSSISRIRQGNLRPPWYMKGTPPGTKASGKDLIASGIDGSMFHLTLLEEDSLHLLRYIQDLYRTKAGIGRHSPQDTENGVRDPRMGHVDGDILVAVLKLGNEWLREMLMKLDETGGSALAGFTELVGRVQGIFQDESDPVNAVMKYMNELVHGVVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNG2</accession>
    <name>D5GNG2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00011278001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-lysyl(27)-[histone H3] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(27)-[histone H3] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60292"/>
            <dbReference type="Rhea" id="RHEA-COMP:15535"/>
            <dbReference type="Rhea" id="RHEA-COMP:15548"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="2.1.1.356"/>
        </reaction>
    </comment>
    <dbReference type="EMBL" id="FN430364">
        <property type="protein sequence ID" value="CAZ86055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841864.1">
        <property type="nucleotide sequence ID" value="XM_002841818.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNG2"/>
    <dbReference type="STRING" id="656061.D5GNG2"/>
    <dbReference type="GeneID" id="9182255"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011278001"/>
    <dbReference type="eggNOG" id="KOG1079">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074460_1_0_1"/>
    <dbReference type="InParanoid" id="D5GNG2"/>
    <dbReference type="OMA" id="MANCTHR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140951">
        <property type="term" value="F:histone H3K27 trimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10519">
        <property type="entry name" value="SET_EZH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026489">
        <property type="entry name" value="CXC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045318">
        <property type="entry name" value="EZH1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041355">
        <property type="entry name" value="Pre-SET_CXC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45747">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE E(Z)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45747:SF4">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE E(Z)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18264">
        <property type="entry name" value="preSET_CXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51633">
        <property type="entry name" value="CXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CXC" evidence="9">
        <location>
            <begin position="12"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="SET" evidence="8">
        <location>
            <begin position="142"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ86055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="258" mass="28956" checksum="CD9EF29919B4501D" modified="2010-06-15" version="1">MPDSEPKQGTKRKKFPVSALPGYPPDCDRTHTHERRTTFQACRHLGQCTNTCPCVKSHVTCEKSCICSPSCPRRWRGCTCKREGRACTLENKCICAKANRECDVDLCHTCGAAEVLDPKNRYDDAFSNNCCQNVALQRDMPKRTLLGRSAVAGFGLFVGEDVKGGVFLGEYKGEVITTEEADRRGKLYDRRGVSFLFNLNSNQVIDATRAGNKLRYVNHSAKKPNCYARVFMANCTHRIGMFARRDLIAGEELFFDYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNG5</accession>
    <name>D5GNG5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00011281001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430364">
        <property type="protein sequence ID" value="CAZ86058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841867.1">
        <property type="nucleotide sequence ID" value="XM_002841821.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNG5"/>
    <dbReference type="STRING" id="656061.D5GNG5"/>
    <dbReference type="GeneID" id="9182258"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011281001"/>
    <dbReference type="eggNOG" id="ENOG502S90D">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096188_0_0_1"/>
    <dbReference type="InParanoid" id="D5GNG5"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02231">
        <property type="entry name" value="cupin_BLL6423-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047142">
        <property type="entry name" value="OryJ/VirC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36156:SF2">
        <property type="entry name" value="CUPIN TYPE-2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36156">
        <property type="entry name" value="SLR2101 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin type-2" evidence="1">
        <location>
            <begin position="97"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ86058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="194" mass="21306" checksum="BB28AA56FE9094FA" modified="2010-06-15" version="1">MSLPTSTPIEGQLSTGLPETFSHITTHDPITKQAVFHSSRPASWKVFDGHQMAFNVVYTTSTFPVDLSNDADVKAHDKMMEEYKLGLVNPGGTVLRMVDFAPGYECIMHRTESLDYGIVLEGKVEALLDSGEKRVVTRGDVVVQRGTMHAWKNPSETEWARMIFVLQESKEVVIGGEVLGENLGAGVEGLPSSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNT4</accession>
    <name>D5GNT4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00011472001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the polyadenylate-binding protein type-1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430368">
        <property type="protein sequence ID" value="CAZ86177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841986.1">
        <property type="nucleotide sequence ID" value="XM_002841940.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNT4"/>
    <dbReference type="STRING" id="656061.D5GNT4"/>
    <dbReference type="GeneID" id="9182812"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011472001"/>
    <dbReference type="eggNOG" id="KOG0123">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_829471_0_0_1"/>
    <dbReference type="InParanoid" id="D5GNT4"/>
    <dbReference type="OMA" id="DIGGHEG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034450">
        <property type="term" value="F:ubiquitin-ubiquitin ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1900.10:FF:000004">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1900.10">
        <property type="entry name" value="c-terminal domain of poly(a) binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036053">
        <property type="entry name" value="PABP-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002004">
        <property type="entry name" value="PABP_HYD_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46276">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46276:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00658">
        <property type="entry name" value="MLLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00517">
        <property type="entry name" value="PolyA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63570">
        <property type="entry name" value="PABC (PABP) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51309">
        <property type="entry name" value="PABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PABC" evidence="3">
        <location>
            <begin position="225"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="70"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="183"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="304"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="89"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="208"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="320"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ86177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="335" mass="34715" checksum="9151E7BE995ED820" modified="2010-06-15" version="1">MNQRMVNGKPLYVALAQRKDVRKSQLEASIQARNQIRIQQQAAAAGMQPAGFLSAQMYYGPGQQAGFMPQSGRGLPFPQPSMMPMTSGPQGGPGGPGSRGPQAGNFVPNAGRPGPQGPIPPGIPQQMYGPPAMGPGAGPGGPGFSPYPPNTLAAAQAQAVAASQGRGRGSVPPAGMAGIQQGIANMNMGPRGPVQPGRGGPRGNLPPQTQPGRPPAPPQIPHVSTSGIDPTLFAASPPQAQKQLLGESLYPKIQRQHPELAGKITGMLLEMDNMELLALLDDEQALIAKVDEALSVYDEYVKTRTGDDIGGHEGATTNDNENKGDEGKKEGEIKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPB2</accession>
    <name>D5GPB2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00011786001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="FN430373">
        <property type="protein sequence ID" value="CAZ86377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842186.1">
        <property type="nucleotide sequence ID" value="XM_002842140.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPB2"/>
    <dbReference type="FunCoup" id="D5GPB2">
        <property type="interactions" value="585"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GPB2"/>
    <dbReference type="GeneID" id="9186617"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011786001"/>
    <dbReference type="eggNOG" id="KOG1165">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019279_1_1_1"/>
    <dbReference type="InParanoid" id="D5GPB2"/>
    <dbReference type="OMA" id="GRGWDYK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14127">
        <property type="entry name" value="STKc_CK1_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000160">
        <property type="entry name" value="Casein kinase I 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000538">
        <property type="entry name" value="Putative Casein kinase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="13"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="310"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="340"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="314"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="353"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="397"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ86377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="454" mass="50658" checksum="66F59961F72EE3F0" modified="2010-06-15" version="1">MSSSSSNVVGVHYRVGKKIGEGSFGVIFEGVNLLNNQHVAIKFEPRKSDAPQLRDEYRTYKILVGCPGIPNVYYFGQEGLHNILVIDLLGPSLEDLFDHCGRRFSIKTVVMVAKQMLSRVQTIHEKNLIYRDIKPDNFLIGRPGTKSANIIHVVDFGMAKQYRDPKTKQHIPYRERKSLSGTARYMSINTHLGREQSRRDDLEALGHVFMYFLRGGLPWQGLKAATNKQKYEKIGEKKQTTAIKELCDGFPEEFNQYLSYVRNLGFEDTPDYDLLRDLFTQALKNTGEVEDGEYDWMKLNNGKGWEAISKHPSSHNYHTAHNQAHGTNGELRLLPPAQVRQPPQLTPSRLNAPHPPPPSPGKPMGPTRGDRQNAPGAMPTKRPSGPAGGLQKPVVTPTASTQAQFQASNTNLPPTTRGPSSQVLNSQQNQPRNNPAEPRRSFVQKLAAAFCCGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>D5GQ05</accession>
    <name>D5GQ05_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00012121001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430380">
        <property type="protein sequence ID" value="CAZ86598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842407.1">
        <property type="nucleotide sequence ID" value="XM_002842361.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GQ05"/>
    <dbReference type="GeneID" id="9185176"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012121001"/>
    <dbReference type="eggNOG" id="ENOG502S9VS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_342616_0_0_1"/>
    <dbReference type="InParanoid" id="D5GQ05"/>
    <dbReference type="OMA" id="NICGREM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="401"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="594"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="677"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="741"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="401"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="415"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="603"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="629"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="748"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="770"/>
            <end position="784"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ86598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="827" mass="86238" checksum="7254447D3CC83F77" modified="2010-06-15" version="1">MDEMEIASSASSRGPEILSNPVIIESILPALEHMNDILSLLLVSRQTYLLVNPLHPFSARKLSFADLAHVKNRHIIEFLYRPLGGVQINSRRRLLEDGRTVTVSGGLEKGYVGAWRFLNSIDLSGTGITTRLVKLLIAATAGGKIPGLLPDRSLDRAPTNEMEHDTYTSRTGLFSKEFESISGGAYRLLADQSLRLEKLCVTGCPGVEIGEINSFLKKILVVVTAPKRRELRLKATQQLAAAQQQQNGGNAGAATNNAGFAAIQQNPQVHAQAQAQAQQQNEDLSTVPSPLPATVEELEEFGLPCLSLRRLELANVAGLYVDPPEKYRGKKEWQYAKECPFGRNVGGLNSIAGCLGIDVDVLFCQGAGCATSFDNSRWRLSHAEGEETHFIYDDEFDVSTLASNPNNQTENLSQASGSSAAAGMAGDSSHHNNGSRNPRKTYHYPLPRLPGERILILDDGRVFRIPRHAIPAPPTAQQPQQQQNVPAPVAPAQLLAAPGLGQGQGNALNVGPGGGGGGAAVGGVAGGGGGIAPSNSQGWSAGQFLQDPAQYGLGAVGSAMPNTSALAHISQIAGLHGIQGLTAVADAASQIITGGGTGAPDDPATLGQQQGQPPLTPSPATDGVLSPNSSPDTPAAGPSATATAQPANTPGDVANASPGDGMNTALLDLMAAVQASTPPFGPTSADPSSPGGGIAPAATGVGGFLPQIPHNHQNIFAQSNFAQQQQPMAQANMHNHFHHPAIPPPPQQTHNQNQPAPGIGVSLGPRRRNNSPGGRTGSGAAAGRPGRRIAMLDGPRGLCEGCGREVWLCENCNRYWVVTCGGCASRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5GQ23</accession>
    <name>D5GQ23_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00012160001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430381">
        <property type="protein sequence ID" value="CAZ86616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842425.1">
        <property type="nucleotide sequence ID" value="XM_002842379.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GQ23"/>
    <dbReference type="GeneID" id="9185196"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012160001"/>
    <dbReference type="HOGENOM" id="CLU_2962576_0_0_1"/>
    <dbReference type="InParanoid" id="D5GQ23"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ86616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="59" mass="6670" checksum="034244D35A39F72A" modified="2010-06-15" version="1">MSRYGDDLERRFKQRLGLADGVSLKPWAIVAVLLVVVFGFMLMRYQNTGDGRTRTGCGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>D5GQ26</accession>
    <name>D5GQ26_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00012166001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430382">
        <property type="protein sequence ID" value="CAZ86619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GQ26"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012166001"/>
    <dbReference type="HOGENOM" id="CLU_2759657_0_0_1"/>
    <dbReference type="InParanoid" id="D5GQ26"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ86619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="70" mass="8130" checksum="89FE4961DE19E986" modified="2010-06-15" version="1">MNAAKSLDCRIPKRWIPPNRPSMQIVLALVSQVSWGERKERARHTTGDKPNNPNRKNNEPRVHLLKHLID</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GQ30</accession>
    <name>D5GQ30_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00012176001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430382">
        <property type="protein sequence ID" value="CAZ86623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GQ30"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012176001"/>
    <dbReference type="HOGENOM" id="CLU_2689602_0_0_1"/>
    <dbReference type="InParanoid" id="D5GQ30"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ86623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="74" mass="8519" checksum="24A542D1BAB68968" modified="2010-06-15" version="1">MDHWTCSSHTTQPLCRRNLVSFLPSFPFPPSLPPSLLLYLLFSRGFFPIFQFRIFHFAAAGGARTCLFEYSMTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5G3W6</accession>
    <name>D5G3W6_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cysteine synthase 1</fullName>
            <ecNumber evidence="5">2.5.1.47</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">O-acetylserine (thiol)-lyase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">O-acetylserine sulfhydrylase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">O-succinylserine sulfhydrylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">GSTUM_00003830001</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the conversion of O-succinyl-L-serine into cysteine, the last step in the cysteine biosynthesis pathway. Can also use O-acetyl-L-serine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate</text>
            <dbReference type="Rhea" id="RHEA:14829"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:58340"/>
            <dbReference type="EC" id="2.5.1.47"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>O-succinyl-L-serine + hydrogen sulfide = L-cysteine + succinate</text>
            <dbReference type="Rhea" id="RHEA:53816"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:136856"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cysteine synthase/cystathionine beta-synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.47" evidence="5"/>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835088.1">
        <property type="nucleotide sequence ID" value="XM_002835042.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G3W6"/>
    <dbReference type="STRING" id="656061.D5G3W6"/>
    <dbReference type="GeneID" id="9187147"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003830001"/>
    <dbReference type="eggNOG" id="KOG1481">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021018_1_0_1"/>
    <dbReference type="InParanoid" id="D5G3W6"/>
    <dbReference type="OMA" id="VVTVFWD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004124">
        <property type="term" value="F:cysteine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01561">
        <property type="entry name" value="CBS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000011">
        <property type="entry name" value="Cysteine synthase (o-acetylserine)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050214">
        <property type="entry name" value="Cys_Synth/Cystath_Beta-Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001216">
        <property type="entry name" value="P-phosphate_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007989">
        <property type="entry name" value="PRK10717.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10314">
        <property type="entry name" value="CYSTATHIONINE BETA-SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00901">
        <property type="entry name" value="CYS_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="19">
        <location>
            <begin position="36"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00050981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00058228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00072087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00078262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00079147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00081847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="CAZ79209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="367" mass="39646" checksum="E911B5CFF78BBB58" modified="2010-06-15" version="1">MFRFGLRRFATAAEAASPYGIKVSKAQGSVNGFVGAIGNTPLIRLGRLSEETGCDIYGKAEFMNPGGSVKDRAALYVVKDAEEKGLIKPGGTIVEGTAGNTGIGLAHVCRSKGYKCVIYMPNTQSRGKIDLLRLLGAEVFPVPAVAFENPENYNHQAKRHAEKTENSVWTNQFDNVANRRAHIETTGPEIWAQLGGKLDGFTCATGTGGTLAGVTRYLKEVSDGRTECWLADPPGSVLFSYITSGGKLNERTGGSITEGIGQGRVTDNLKPEIKLINNALHISDEKSIAMVYRLLDEEGLYMGASSALNVVAAYELAKKLGPGKSVATILCDGAYRYADRLFSKKWLDSKGLYEAIPEHLRKYIVLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4E4</accession>
    <name>D5G4E4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00004072001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class myotubularin subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835266.1">
        <property type="nucleotide sequence ID" value="XM_002835220.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4E4"/>
    <dbReference type="STRING" id="656061.D5G4E4"/>
    <dbReference type="GeneID" id="9185012"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004072001"/>
    <dbReference type="eggNOG" id="KOG1089">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1099167_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4E4"/>
    <dbReference type="OMA" id="ANDIWIL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004438">
        <property type="term" value="F:phosphatidylinositol-3-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030564">
        <property type="entry name" value="Myotubularin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010569">
        <property type="entry name" value="Myotubularin-like_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048994">
        <property type="entry name" value="PH-GRAM_MTMR6-9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807">
        <property type="entry name" value="MYOTUBULARIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807:SF128">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-3,5-BISPHOSPHATE 3-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06602">
        <property type="entry name" value="Myotub-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21098">
        <property type="entry name" value="PH-GRAM_MTMR6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51339">
        <property type="entry name" value="PPASE_MYOTUBULARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myotubularin phosphatase" evidence="2">
        <location>
            <begin position="140"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ79387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="253" mass="28921" checksum="3F6F72C088208B1F" modified="2010-06-15" version="1">MEYIRDFKVENVTILHHGQPTVGTLHLTPHHLIFRIDPEAESSSQSQAPPAQTKPAKRPREIWITYPVVSTLFRHPASAASPAHLRLRNRDFSFLTFQFKTERECRDVFESIKALTIVKGVESLYAYSYAPGSIEKKFNGWKIYNPLKEYERMGIGTEKCKGWRMSKINKDFAFSPTYPALLVVPATISDTTLSHAKNYRSRARIPALTYIHPLNNCSITRCSQPLTGVRGNRSIQDEKLNPLQKYTGRNKTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4M8</accession>
    <name>D5G4M8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">UDP-galactose transporter homolog 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00000016001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleotide-sugar transporter family. SLC35B subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN429989">
        <property type="protein sequence ID" value="CAZ79471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835314.1">
        <property type="nucleotide sequence ID" value="XM_002835268.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4M8"/>
    <dbReference type="FunCoup" id="D5G4M8">
        <property type="interactions" value="450"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G4M8"/>
    <dbReference type="GeneID" id="9188408"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000016001"/>
    <dbReference type="eggNOG" id="KOG1581">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036019_0_2_1"/>
    <dbReference type="InParanoid" id="D5G4M8"/>
    <dbReference type="OMA" id="CGAIGQV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005459">
        <property type="term" value="F:UDP-galactose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005460">
        <property type="term" value="F:UDP-glucose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120112">
        <property type="term" value="P:UDP-glucose transmembrane transport into endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013657">
        <property type="entry name" value="SCL35B1-4/HUT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10778">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10778:SF10">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08449">
        <property type="entry name" value="UAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="140"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="207"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="245"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="285"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="323"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ79471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="375" mass="40956" checksum="12E45CF9F8EEA19D" modified="2010-06-15" version="1">MARRKNPPPEDANDPIVAIAGSVAHKAESSKDHGSSIFQLIICVGGIYASFLTWALLQERITTTPYGADKKIFRSPLVLNTVQSLFAALTGYAYFSYSSSPGQPSHVFPNFSISLRLLLVALTQSLASPFGYASLQHIDYITYILAKSCKLLPVMFLHITIFRRRYPLYKYAVVFLVTAGVAVFTLYPAHPKKIKKSASSNGEKKFYGMLLLGVNLLFDGLTNTIQDDIFSRTPKGAVSGPQMMTALNTISSVLTIGFLLLNPWSTELNDALAFVKEHPKVGMDIMGFAVCGGLGQVFIFHTLSSFGSLVLVTVTVTRKMLSMIFSVVAFGHSLSSMQWLGVGLVFGGIGAEAEMKRQSEATKKMTKQNGHTKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>D5G500</accession>
    <name>D5G500_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00000217001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EBP family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429994">
        <property type="protein sequence ID" value="CAZ79593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835436.1">
        <property type="nucleotide sequence ID" value="XM_002835390.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G500"/>
    <dbReference type="STRING" id="656061.D5G500"/>
    <dbReference type="GeneID" id="9186301"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000217001"/>
    <dbReference type="eggNOG" id="KOG4826">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072128_1_0_1"/>
    <dbReference type="InParanoid" id="D5G500"/>
    <dbReference type="OMA" id="VYLWLYL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047750">
        <property type="term" value="F:cholestenol delta-isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016125">
        <property type="term" value="P:sterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007905">
        <property type="entry name" value="EBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033118">
        <property type="entry name" value="EXPERA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14207:SF1">
        <property type="entry name" value="EMOPAMIL-BINDING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14207">
        <property type="entry name" value="STEROL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05241">
        <property type="entry name" value="EBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51751">
        <property type="entry name" value="EXPERA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="52"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="158"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="196"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="EXPERA" evidence="8">
        <location>
            <begin position="47"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ79593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="246" mass="27615" checksum="9F1AA1DD10754111" modified="2010-06-15" version="1">MTVGAAPPSLLQVIDGTTILSLLFFTSLLLSSYLSTKYFLPVGTSAKTRFLYGWHIFDALIHILFECPFLYISFFSSAPTGLTGNSANVLWGDLSRAYGAEYATGLFGHLWREYAKADRRWAHADVGVLALELPTGLLGGPLAWWVSELIRRKNRSAWFWMLVLAVAELYGGWMTFAPEWLTGSQNLNTSNPMHTWLYLFFMNVIWVIVPGWICFEAYTEMVVTPSKGRITTIHHDDTSAAGKKSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5C9</accession>
    <name>D5G5C9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00004273001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SF3A3 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835565.1">
        <property type="nucleotide sequence ID" value="XM_002835519.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5C9"/>
    <dbReference type="FunCoup" id="D5G5C9">
        <property type="interactions" value="1188"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G5C9"/>
    <dbReference type="GeneID" id="9188141"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004273001"/>
    <dbReference type="eggNOG" id="KOG2636">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027160_1_0_1"/>
    <dbReference type="InParanoid" id="D5G5C9"/>
    <dbReference type="OMA" id="GPKAFQK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000690">
        <property type="entry name" value="Matrin/U1-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051421">
        <property type="entry name" value="RNA_Proc_DNA_Dmg_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031774">
        <property type="entry name" value="SF3A3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024598">
        <property type="entry name" value="SF3a60/Prp9_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021966">
        <property type="entry name" value="SF3a60_bindingd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022755">
        <property type="entry name" value="Znf_C2H2_jaz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12786:SF2">
        <property type="entry name" value="SPLICING FACTOR 3A SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12786">
        <property type="entry name" value="SPLICING FACTOR SF3A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16837">
        <property type="entry name" value="SF3A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12108">
        <property type="entry name" value="SF3a60_bindingd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11931">
        <property type="entry name" value="SF3a60_Prp9_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12171">
        <property type="entry name" value="zf-C2H2_jaz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50171">
        <property type="entry name" value="ZF_MATRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Matrin-type" evidence="9">
        <location>
            <begin position="406"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="271"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ79722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="501" mass="58574" checksum="65F65130FABA2601" modified="2010-06-15" version="1">MGILDEQRSLCEDLERLEQACADRILEEPKSARERLTRDHQIAKFVDRFQQQSARLLETQADRNGTRQKEVDSLSSKDPFEEFYRQLGQIKDFHRRYPNEPVENLERAYKRRQATDGEYVSEIDTMFTGEEAHGKFFDLTKNHEDYLNLPGVKRITYLSYLSLFDKFDKFARNQKMNDKYFKYVSALAEYLESFLSRTRPLENPEGIVEQTGKDFEKAWEEDNVVGWGRLEKSDGDIGPRQEEMRVPKEFYCDVCAKGFKNNNVFDHHFNGKQHKKAAAKQAEGSAENGEARKAGGSSGAASRLKDKAIAEREWRIVKLTEVMSKERDETMTNVERKQSLTERERQMELDALYEENSADPNIEAETHTDDENDDEKIYNPLKLPLAWDGKPIPYWLYKLHGLGVEFSCEICGNFVYMGRRAFDKHFNEYRHTYGLRCLGITNTTLFREITKIDEAQRLWEKLENDKKKTKMANETVEEMEDGQGNVMPRKVYEDLQKQGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5N1</accession>
    <name>D5G5N1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00001496001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430000">
        <property type="protein sequence ID" value="CAZ79824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835667.1">
        <property type="nucleotide sequence ID" value="XM_002835621.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5N1"/>
    <dbReference type="GeneID" id="9187501"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001496001"/>
    <dbReference type="HOGENOM" id="CLU_2905791_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5N1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ79824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="62" mass="7033" checksum="4041EF219C3DCE2F" modified="2010-06-15" version="1">MIQYKKSMVTAVWCERSHETQRLDEPYPWNSPRLGSLPQPIIPVVQRAPGTSQPGPRSSDVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5N4</accession>
    <name>D5G5N4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ER membrane protein complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00001500001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the ER membrane protein complex (EMC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
            <orientation evidence="4">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the EMC2 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430000">
        <property type="protein sequence ID" value="CAZ79827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835670.1">
        <property type="nucleotide sequence ID" value="XM_002835624.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5N4"/>
    <dbReference type="FunCoup" id="D5G5N4">
        <property type="interactions" value="169"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G5N4"/>
    <dbReference type="GeneID" id="9187504"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001500001"/>
    <dbReference type="eggNOG" id="KOG3060">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052388_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5N4"/>
    <dbReference type="OMA" id="LMEMLFY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039856">
        <property type="entry name" value="EMC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055217">
        <property type="entry name" value="TPR_EMC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12760">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22890">
        <property type="entry name" value="TPR_EMC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="EMC2 TPR-like" evidence="5">
        <location>
            <begin position="98"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="155"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF22890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ79827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="309" mass="34778" checksum="A274641EB35E7E06" modified="2010-06-15" version="1">MAMLKLLLSYLSIHPSPLRNPSTSLHLSQQAPAFLSSSESTKDTQETWLIYEQLLLSCLRTGDDSSARVCLQRLSGRFGVDSARVKALTGLYDEATSNSRGELESVLQRYQDILKEDPTNMPIEKRRISLLLSLGRTEDGIEALTSLLSHSPNDAESWAHLSSLYFTQGLYQQSVFCLEEVLLILPNAYNIFARIGEVTYISASAGASGTNQTEAFEEAIKYFARSVELSEWYLRGWYGLKLVTGKFIQAKPSDGTLRKMESLNQLATEKLLQIVGRFRRNEKGWMGFDEAEILAAKNLIDRDEQKFSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5Q1</accession>
    <name>D5G5Q1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Altered inheritance of mitochondria protein 41</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">AIM41</name>
        <name evidence="2" type="ORF">GSTUM_00001429001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AIM41 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430001">
        <property type="protein sequence ID" value="CAZ79844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835687.1">
        <property type="nucleotide sequence ID" value="XM_002835641.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5Q1"/>
    <dbReference type="FunCoup" id="D5G5Q1">
        <property type="interactions" value="81"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G5Q1"/>
    <dbReference type="GeneID" id="9187522"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001429001"/>
    <dbReference type="eggNOG" id="ENOG502S6DQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079430_0_1_1"/>
    <dbReference type="InParanoid" id="D5G5Q1"/>
    <dbReference type="OMA" id="AMGAVMK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016884">
        <property type="term" value="F:carbon-nitrogen ligase activity, with glutamine as amido-N-donor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1510.10">
        <property type="entry name" value="Uncharacterised protein YqeY/AIM41 PF09424, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003789">
        <property type="entry name" value="Asn/Gln_tRNA_amidoTrase-B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019004">
        <property type="entry name" value="YqeY/Aim41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042184">
        <property type="entry name" value="YqeY/Aim41_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28055">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 41, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28055:SF1">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 41, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09424">
        <property type="entry name" value="YqeY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89095">
        <property type="entry name" value="GatB/YqeY motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU365099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="176" mass="19012" checksum="A7E7936E7D840372" modified="2010-06-15" version="1">MLSRAILRSIRVPVLRVYSTAQPPSLILKLRNDLKDAMRNKDTNRLNVIRGILSDISNASKTTNPPSTDLDILALINETKTKSAASIAEFREAGREDLVEKETGQVTILDIYAGMVQRVGKEEITAVVNGVIRAAKEAGEKVDIGNVIKKALAELAGKPLVKAEVVAIAKEAIKTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5R3</accession>
    <name>D5G5R3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00001446001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL9 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430001">
        <property type="protein sequence ID" value="CAZ79856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835699.1">
        <property type="nucleotide sequence ID" value="XM_002835653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5R3"/>
    <dbReference type="STRING" id="656061.D5G5R3"/>
    <dbReference type="GeneID" id="9187534"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001446001"/>
    <dbReference type="eggNOG" id="ENOG502SA1R">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067878_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5R3"/>
    <dbReference type="OMA" id="RNRWFPA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.10">
        <property type="entry name" value="Ribosomal protein L9, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000244">
        <property type="entry name" value="Ribosomal_bL9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020070">
        <property type="entry name" value="Ribosomal_bL9_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036935">
        <property type="entry name" value="Ribosomal_bL9_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21368">
        <property type="entry name" value="50S RIBOSOMAL PROTEIN L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01281">
        <property type="entry name" value="Ribosomal_L9_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein L9" evidence="4">
        <location>
            <begin position="37"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ79856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="220" mass="24184" checksum="C96FB41202B15AFC" modified="2010-06-15" version="1">MAFFSIPSCPTCIRRLAGGALQQIRGKKRTRKVPTITVQLTRDVPKFGKKGTVLQASRGRMRVVFRFPQGVADYVTRHMLKSMPAGTLKPRDPMFMNVAVEKKKPKVAIELLTPTQTIALLDSYLPPNLMFSRSTIAPNESAIHGSVTLTDIATAVRNIAVANGKEGSRIVVTPENVKFAGKGVEGDKLKSLGTFDFEISVKGANDFVRRKVMIVKQEEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5R5</accession>
    <name>D5G5R5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00001448001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430001">
        <property type="protein sequence ID" value="CAZ79858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835701.1">
        <property type="nucleotide sequence ID" value="XM_002835655.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5R5"/>
    <dbReference type="ESTHER" id="tubmm-d5g5r5">
        <property type="family name" value="Fusarinine_C_esterase_sidJ"/>
    </dbReference>
    <dbReference type="GeneID" id="9187536"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001448001"/>
    <dbReference type="eggNOG" id="KOG4840">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049633_3_0_1"/>
    <dbReference type="InParanoid" id="D5G5R5"/>
    <dbReference type="OMA" id="PPWVNKE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013744">
        <property type="entry name" value="SidJ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31591:SF7">
        <property type="entry name" value="DUF1749-DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31591">
        <property type="entry name" value="UPF0613 PROTEIN PB24D3.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08538">
        <property type="entry name" value="DUF1749"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ79858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="301" mass="32910" checksum="2DC35860933DC801" modified="2010-06-15" version="1">MDQEFFYPSSRATILHHYLPRLIAIEHPAAINGNMKNTLLFVGGLGDGIHTIPFVDPVIDAATGAGWSMVEVLLSSSYKGWGIGSIAQDAEEISACVKYFRTIRGGKLVLMGHSTGCQDALEYLTKHSAVVNGVIFQASVSDRESATLNRSPDHLSASLTHARSLIDSGRGAEIIPSHFRSPGYTDTPVCAQRWWDLNAFAGADDYFSSDLNEEMLTRTFGMIMRGTNVLFLFSAMDQFVPAWVDKEYLLKRWYLACALGGAVVDKVESGVINGANHNFEGVDANVLADMIGRVIRFLGKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5T2</accession>
    <name>D5G5T2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00001468001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430001">
        <property type="protein sequence ID" value="CAZ79875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5T2"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001468001"/>
    <dbReference type="HOGENOM" id="CLU_3419472_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5T2"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ79875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="25" mass="3065" checksum="B5AC84B611CE88F1" modified="2010-06-15" version="1">MILIIKHVPAEQILPRNKYVRSCRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5V4</accession>
    <name>D5G5V4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00001591001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430002">
        <property type="protein sequence ID" value="CAZ79897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835740.1">
        <property type="nucleotide sequence ID" value="XM_002835694.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5V4"/>
    <dbReference type="STRING" id="656061.D5G5V4"/>
    <dbReference type="GeneID" id="9187575"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001591001"/>
    <dbReference type="eggNOG" id="ENOG502RZZS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038616_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5V4"/>
    <dbReference type="OMA" id="RYADVQP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="190"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="377" mass="41552" checksum="A2859A0278C55F06" modified="2010-06-15" version="1">MANTTPPPPALLELLPPLLAHILIAFTSPQPPPSLYPLLSPILRQRLRLLRVPPSSTPSVEETWLSLLTWSSTAGPMLSAHLATQDFAPHPASGELELGEIDFKGFQQLDKETIKACVQLPERGIEAVYLWITNDPDGEDDGWRIMDLHLSSDSEQTEWCPTVREADEAFYSRSAKPVLPVTNPIPSYANDALRADNDDDDDGYWDMYDRTPARSPMLPQKVDQMADDDYYSQYDHTQPAMEPEDSSANSPLEYSRYTNPLTMQHSSSHSPTTVSSTPPTAMSASFLIDKEALAGVVTHAPCPVSAGPSSSSAESSVGKLEEAAGLTMQAEMGVKQHISTSIKSLYRLSKSVGISREEFERLLQTEIAFLEMMDEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>D5G694</accession>
    <name>D5G694_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Histone acetyltransferase GCN5</fullName>
            <ecNumber evidence="3">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">GSTUM_00001663001</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acetyltransferase family. GCN5 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="3"/>
    <dbReference type="EMBL" id="FN430008">
        <property type="protein sequence ID" value="CAZ80037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835880.1">
        <property type="nucleotide sequence ID" value="XM_002835834.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G694"/>
    <dbReference type="FunCoup" id="D5G694">
        <property type="interactions" value="419"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G694"/>
    <dbReference type="GeneID" id="9187250"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001663001"/>
    <dbReference type="eggNOG" id="KOG1472">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015741_1_1_1"/>
    <dbReference type="InParanoid" id="D5G694"/>
    <dbReference type="OMA" id="HQPPKEW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140671">
        <property type="term" value="C:ADA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046695">
        <property type="term" value="C:SLIK (SAGA-like) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140068">
        <property type="term" value="F:histone crotonyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036408">
        <property type="term" value="F:histone H3K14 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043993">
        <property type="term" value="F:histone H3K18 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140129">
        <property type="term" value="F:histone H3K56ac reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043992">
        <property type="term" value="F:histone H3K9 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140011">
        <property type="term" value="F:histone H4K12ac reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140046">
        <property type="term" value="F:histone H4K16ac reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010515">
        <property type="term" value="P:negative regulation of induction of conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045815">
        <property type="term" value="P:transcription initiation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05509">
        <property type="entry name" value="Bromo_gcn5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000046">
        <property type="entry name" value="Histone acetyltransferase GCN5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000004">
        <property type="entry name" value="Histone acetyltransferase KAT2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037800">
        <property type="entry name" value="GCN5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45750">
        <property type="entry name" value="GH11602P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45750:SF3">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0010">Activator</keyword>
    <keyword evidence="12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8 14" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="17">
        <location>
            <begin position="54"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="16">
        <location>
            <begin position="296"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="25"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAZ80037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="392" mass="45052" checksum="7B8AAC6CD9A2BC7E" modified="2010-06-15" version="1">MPAAKNGDIKRKSEDTGDLSPKRIKVSESPEESKKPKALFRESNAVLEERNGDIEFRVVNNDNSSQSTIILTGLKNIFQKQLPKMPRDYIARLVYDRTHLSMAIVKKPLEVVGGITYRPFKGRQFAEIVFCAISSDQQVRGYGAHLMSHLKDYVKATSDVMHFLTYADNYAIGYFKKQGFTKEITLDKHKWMGYIKDYEGGTIMQCSMIPRIRYLEAGKMLAKQKEAVHLKTRSISKSHIIHPAPKAFKNGPCKIDPTSIAAIKDAGWSPEMDELARQPKHGPHYGQLLHILNEMQNHQASWPFQKPVSQDEVPDYYNVITEPMDLETMEKRLEQDAYGSPEDFVRDAKLIFTNCRRYNNESTSYWKNANKLEKFMNSKLKEIPEWSVRNPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6E1</accession>
    <name>D5G6E1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 15">Squalene synthase</fullName>
            <shortName evidence="15">SQS</shortName>
            <shortName evidence="15">SS</shortName>
            <ecNumber evidence="4 15">2.5.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">GSTUM_00004443001</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes the condensation of 2 farnesyl pyrophosphate (FPP) moieties to form squalene.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>2 (2E,6E)-farnesyl diphosphate + NADH + H(+) = squalene + 2 diphosphate + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:32299"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15440"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
            <dbReference type="EC" id="2.5.1.21"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>2 (2E,6E)-farnesyl diphosphate + NADPH + H(+) = squalene + 2 diphosphate + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:32295"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15440"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
            <dbReference type="EC" id="2.5.1.21"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="15">Terpene metabolism; lanosterol biosynthesis; lanosterol from farnesyl diphosphate: step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the phytoene/squalene synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.21" evidence="4 15"/>
    <dbReference type="EMBL" id="FN430009">
        <property type="protein sequence ID" value="CAZ80084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835927.1">
        <property type="nucleotide sequence ID" value="XM_002835881.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6E1"/>
    <dbReference type="FunCoup" id="D5G6E1">
        <property type="interactions" value="283"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G6E1"/>
    <dbReference type="GeneID" id="9181169"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004443001"/>
    <dbReference type="eggNOG" id="KOG1459">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031981_2_1_1"/>
    <dbReference type="InParanoid" id="D5G6E1"/>
    <dbReference type="OMA" id="GEACQLM"/>
    <dbReference type="UniPathway" id="UPA00767">
        <property type="reaction ID" value="UER00751"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055056">
        <property type="term" value="F:D-glucose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051996">
        <property type="term" value="F:squalene synthase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045338">
        <property type="term" value="P:farnesyl diphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902767">
        <property type="term" value="P:isoprenoid biosynthetic process via mevalonate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00683">
        <property type="entry name" value="Trans_IPPS_HH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.600.10:FF:000003">
        <property type="entry name" value="Farnesyl-diphosphate farnesyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002060">
        <property type="entry name" value="Squ/phyt_synthse"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006449">
        <property type="entry name" value="Squal_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019845">
        <property type="entry name" value="Squalene/phytoene_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044844">
        <property type="entry name" value="Trans_IPPS_euk-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033904">
        <property type="entry name" value="Trans_IPPS_HH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01559">
        <property type="entry name" value="squal_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11626">
        <property type="entry name" value="FARNESYL-DIPHOSPHATE FARNESYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11626:SF2">
        <property type="entry name" value="SQUALENE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00494">
        <property type="entry name" value="SQS_PSY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01018">
        <property type="entry name" value="Squalene/Phytoene_Synthase_Lik"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01044">
        <property type="entry name" value="SQUALEN_PHYTOEN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01045">
        <property type="entry name" value="SQUALEN_PHYTOEN_SYN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="14" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="10" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="13" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="6 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="415"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU368088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAZ80084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="472" mass="53755" checksum="B7C5440F511993AA" modified="2010-06-15" version="1">MKTSDILYLLAHPNQLRAIIQWKVWHNPLHQRKIENESLTLRRCFEFLDLTSRSFSAVIQELCPELCVSVTLFYLILRGLDTIEDDMTIPLEIKEPLLRSFDKVLDKEGWTFDGNGPKEKDKQLLVEFSNVITEFALLSESHRAIIKDITKKMGNGMADYANNAEHNVNGVNTIADYDLYCHYVAGLVGDGLTRLFVDTGKANPALLERPHLINSMGLFLQKTNIIRDYREDLDDKRRFWPKEIWTKHIEKYEELHLPENEEAALNCISEMCLNSLQHVDECLFYLAGIKDQSVFNFTAIPQVMAIATLALVFRNKDVFHKNVKIPKGEACELMIEAGNLRSTCDIFRKYMKIIHKKNTPKDPNFLNISVTIGKVEQFIETIFPTAVEPRVVAQRAQMRATGETPEQAAAAKKEMMFLICAIVATLSVISGVMIFAAWLMGARFDIAFQELRKGNFGPPGYNPVADARHGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6H8</accession>
    <name>D5G6H8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00004488001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430009">
        <property type="protein sequence ID" value="CAZ80121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835964.1">
        <property type="nucleotide sequence ID" value="XM_002835918.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6H8"/>
    <dbReference type="FunCoup" id="D5G6H8">
        <property type="interactions" value="100"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G6H8"/>
    <dbReference type="GeneID" id="9181206"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004488001"/>
    <dbReference type="eggNOG" id="KOG0724">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019916_1_0_1"/>
    <dbReference type="InParanoid" id="D5G6H8"/>
    <dbReference type="OMA" id="RNHTWSE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0101031">
        <property type="term" value="C:protein folding chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051083">
        <property type="term" value="P:'de novo' cotranslational protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000054">
        <property type="term" value="P:ribosomal subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006452">
        <property type="term" value="P:translational frameshifting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23953">
        <property type="entry name" value="zuotin_NTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.840">
        <property type="entry name" value="Ribosome-associated complex head domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032003">
        <property type="entry name" value="RAC_head"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042569">
        <property type="entry name" value="RAC_head_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054076">
        <property type="entry name" value="ZUO1-like_ZHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044634">
        <property type="entry name" value="Zuotin/DnaJC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058871">
        <property type="entry name" value="Zuotin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43999">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43999:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16717">
        <property type="entry name" value="RAC_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21884">
        <property type="entry name" value="ZUO1-like_ZHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26185">
        <property type="entry name" value="Zuotin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="5">
        <location>
            <begin position="100"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="167"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="243"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="321"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="167"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="321"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ80121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="442" mass="50731" checksum="38AD02DE25B95EA6" modified="2010-06-15" version="1">MASVKIPKPLPTLPEDWNSESNFIVHAPLSPAVHRNLSPAGPHFLAHARRKRHSRTFSEDDRIQAQQNVKNVEDEDAGEISEAESPVLLSRDPKDWKNQDHYAVLGLSKYRYKATEEQIKKAHRKKVLKHHPDKKAAVGAVDDDSFFKCIQKAMEILSDPVKRRQFDSVDEGANRDPPSKKSKGNFYKGWGPVFESEGRFSRKQPVPKLGDDKSIKAEVEEFYNFWYNFDSWRTFEYLDEEVPDDNENRDQKRYIQRKNNASRAKRKTEDTARLRKLVDDALSLDTRIKKFREEEKKLRNAKKDAREAEEKRLAEEARIKAEEDKKRKEEEEAREKAQKADSKKAREAAKNAVKKNRRVLKGSVKDVNYFASGDATPAVIDRVLGEVELVISKIDNEDLQTLARNLSGTRGADAIKSTWVEAVGELVKKGNAKVEDFKVFTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6I0</accession>
    <name>D5G6I0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">IMP-specific 5'-nucleotidase 1</fullName>
            <ecNumber evidence="12">3.1.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00004490001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">IMP-specific 5'-nucleotidase involved in IMP (inositol monophosphate) degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>IMP + H2O = inosine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:27718"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17596"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="EC" id="3.1.3.99"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3 12">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the ISN1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.-" evidence="12"/>
    <dbReference type="EMBL" id="FN430009">
        <property type="protein sequence ID" value="CAZ80123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835966.1">
        <property type="nucleotide sequence ID" value="XM_002835920.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6I0"/>
    <dbReference type="FunCoup" id="D5G6I0">
        <property type="interactions" value="85"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G6I0"/>
    <dbReference type="GeneID" id="9181208"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004490001"/>
    <dbReference type="eggNOG" id="ENOG502QR24">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031816_1_0_1"/>
    <dbReference type="InParanoid" id="D5G6I0"/>
    <dbReference type="OMA" id="WGVLACQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008253">
        <property type="term" value="F:5'-nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006190">
        <property type="term" value="P:inosine salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071590">
        <property type="term" value="P:nicotinamide riboside biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071592">
        <property type="term" value="P:nicotinic acid riboside biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009453">
        <property type="entry name" value="ISN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28213">
        <property type="entry name" value="IMP-SPECIFIC 5'-NUCLEOTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28213:SF1">
        <property type="entry name" value="IMP-SPECIFIC 5'-NUCLEOTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06437">
        <property type="entry name" value="ISN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028836">
        <property type="entry name" value="ISN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 12" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR028836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ80123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="424" mass="47604" checksum="9211AE5486DD3F7C" modified="2010-06-15" version="1">MTTRYRVEYALKAHRRDALIEFIKGLLAVPFVLHSQPTTVAGDASFRVMAVEAGRRYREIMKDVEELINDHITYQRAGTPERSKLRFLVPSVGTFFTPLLLEKAFVAQDARRSISNRRFVAPSFNDIRLILNTAQIMSLTTLDGPLKLVTFDGDVTLYDDGQCLVPTNPVIPRILQLMQRGLYIGIVTAAGYTDSVKYMERLHGLLDAIKFSKDLTEGQKSNLIILGGESSYLYKSCPTSPTLLVAEPRENWELDEMRTWSETDIQRLLDIAETALRESISTMKLEAIVLRKERAVGIIPAIPDVKFEREQLEEAVLAAQKTLELSDIGRRLPFCAFNGGNDVFVDIGDKRLGVRCCQRFFGNISGSQTLHIGDQFLSAGHNDFKARMAGTTVWVASPAETVSSLDELWDLLEHGMPKGYDHDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6I3</accession>
    <name>D5G6I3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00004493001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430009">
        <property type="protein sequence ID" value="CAZ80126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835969.1">
        <property type="nucleotide sequence ID" value="XM_002835923.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6I3"/>
    <dbReference type="GeneID" id="9181211"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004493001"/>
    <dbReference type="HOGENOM" id="CLU_263243_0_0_1"/>
    <dbReference type="InParanoid" id="D5G6I3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="294"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="451"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="516"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="643"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="714"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="294"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="333"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="476"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="529"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="668"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="793"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="815"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="834"/>
            <end position="843"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1279" mass="139464" checksum="6B72A15B52205F91" modified="2010-06-15" version="1">MAVKGDNKAGAVNAPRPSPKPPVAYSDHVHSIAHSKERNDWRNRRPLLTTHGTRTAPREAKAELSQLYDEAGDSKHGGGVSLEIPGPKASVADTLPPNFHATGPKVDVASNTSNATRADNNARSISTGPAKTACQARPATRDSSGYGSSSSGGCGFRNSRAFPPATADPERVMTSDSKHLMEVSQTISGVIEPKPPISSSLKEFLKTPCAEGFGCNMEEDDLFQRSSGGVRLLDNFARQYASDPDAIQRKYGRLCFLLRADPGRGFPRLRYIVETHRSMFWQLDPVRLRGVDSGKIKPEANPRDETTGALVRKTSSGISIPDQDSLGWESRDVSQNLHHSHNQVVRTTSEYTVSTGRAPPRPAEAAPKEHEGGYSSKNLPSHGDRVCATPGGRRGAHSGNRCNGQSQAILMDVDRMPLGRKGGPFSDYSGATTVVSTRTLTGRDKAATVESLDLTTSKHEGLLPENNATFYPNARPPERANDESSKESEEGGIQLDPVVKKQVARDVYTSCGAPGIAAGAVLPPSPKDSAPSTSSGPSSLPSSPPSGSAPPLEVRHQPTGPGDGGSLTAGKTGDCIESAEGELIKSRSSVAKEFSTGEITDYAGMAVPQISEKLLGPPGGSRGSCFASRDEAKMALAVSLQKPGQFATKGEPDPQQSDPEIQEVPSWMNESDSGNETPSVWEWEWDKKSSQTGLTDDEWNVGVPLDRAIIENFDGAAENKTESPVSLGERVFELPEDPGSVPNPRNHEAGKSRYAKKYRAKESKGSSGASEGKDCESPMLANHASEPQAPGDHVVKQPVPEHKASKPPAPENKSRKPFVSHHKVSKPPVPVNRANESTVSAGVNGNLVASGGHSRDPPRSRNNGDAGAPTFRGRPSGGCGGIKKLPEEILHAIFSYAADGVLPEVQVKTRDLTYGSSFEVPSSFNKTRYKGLQSVLRTCRHWRTLGQEILYKYVNLNEWSNLELFARTLSHDRDIGALVRSLRVSVPPFGLRASYQSYNNRDRNSRAVVAEQVDAFHLLPDIMQGCSLIHVMSIDMPNAVPVFNKLVSAQKFPNLREIRMKDRGEKIATGERVWENIIRNAPELRRLLITQDQDLSLRNAPTYIRIPGNIFHCEKASTFKLTQIHLTRSYQISDDILLLLCRKLSNLNDLDIVDCPLVTSRGIARVLEKMPNQLTRLSFWIWINHYGNVSKREQEIGVDLCHALSKYGQSLQHMDIKIFLACHHLWQDGFRELQRCRIVPVHYHDCVKQENIRSSSFFKNVLDKGRSAGKFPALTECRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6L6</accession>
    <name>D5G6L6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00002097001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430011">
        <property type="protein sequence ID" value="CAZ80159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836002.1">
        <property type="nucleotide sequence ID" value="XM_002835956.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6L6"/>
    <dbReference type="STRING" id="656061.D5G6L6"/>
    <dbReference type="GeneID" id="9187185"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002097001"/>
    <dbReference type="eggNOG" id="KOG1209">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_9_1"/>
    <dbReference type="InParanoid" id="D5G6L6"/>
    <dbReference type="OMA" id="CNLYNAS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05374">
        <property type="entry name" value="17beta-HSD-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051911">
        <property type="entry name" value="SDR_oxidoreductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43976">
        <property type="entry name" value="SHORT CHAIN DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43976:SF16">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE_REDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ80159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="287" mass="31257" checksum="75A5C3D1DF44A14C" modified="2010-06-15" version="1">MSAQQRVWLVTGTTSGIGENIALEALSRGDLVVASSRNPSRLTKLTKAGAKAVKIDQNQDDATIRKEVEAALAVYGRIDILVNNAAYVHTGTIEETTDQEIKSQFQANVFGPTSIIRALLPHMRARRSGVIANVGSMASWFPVPACNLYDASKAALRLIGDGLNSELARFGIKITTIEPGMFRTELLDPNVNFIKTSDDKRIEDYREMNETTDGMFRNAHGKQMGDPKRGAEVIYEVLTQTGVAQGREIPLVLALGSDAVSTIQKFARDQADLVKSWGEVSSSTDFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6N6</accession>
    <name>D5G6N6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00002142001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430012">
        <property type="protein sequence ID" value="CAZ80179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836022.1">
        <property type="nucleotide sequence ID" value="XM_002835976.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6N6"/>
    <dbReference type="STRING" id="656061.D5G6N6"/>
    <dbReference type="GeneID" id="9187205"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002142001"/>
    <dbReference type="eggNOG" id="ENOG502S92C">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_832064_0_0_1"/>
    <dbReference type="InParanoid" id="D5G6N6"/>
    <dbReference type="OMA" id="MESSVWP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39610:SF2">
        <property type="entry name" value="BZIP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39610">
        <property type="entry name" value="BZIP DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="310"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="27"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="71"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="95"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="244"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="313"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="334" mass="35112" checksum="0A3A96B204EDCD33" modified="2010-06-15" version="1">MPDVNKLEAVAGTTTTTNASPLTVQFTTTTTTPAPPSATAAGPATATTTTTIMPRTSTEAHSPLPSPIFQTSRPTTPSPRASSSNLHHYPPQHHPSISSRRPSSSLSIGSSSSRRPSSSILSPSSVDQSLPQRLPRQPTPQDAAAFAELHQELETEQEAQVNRLLNMIRLQQLQQQSQNQGSGGSVIDDNCSTAATVDSNSTLGAAVGSGRALSPVSAYSRSGRLSRENSTTSASRGRRRRSGSRTSTATSTSSPSFPTGSFIPDNASDTSASVPWLGSSHSDMGFYIAEAQMLTRENEMLKRRIRELERQVSELSSKSSTAGSDVASPGGTTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5G775</accession>
    <name>D5G775_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00002499001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430022">
        <property type="protein sequence ID" value="CAZ80368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836177.1">
        <property type="nucleotide sequence ID" value="XM_002836131.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G775"/>
    <dbReference type="GeneID" id="9185266"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002499001"/>
    <dbReference type="HOGENOM" id="CLU_2185860_0_0_1"/>
    <dbReference type="InParanoid" id="D5G775"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ80368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="109" mass="12202" checksum="2DE2E42F0A761B7F" modified="2010-06-15" version="1">MLQHHLLPYHLYGPYTPNFTLPDLWEYHPRGPFGINAGKPARVVLGVVIAVDLGRQVFPPGKIHALNEKLFSVRKNPRNNTTGITIHTSSTLAKPLKQFPGFGLVLKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5G790</accession>
    <name>D5G790_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00002516001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430022">
        <property type="protein sequence ID" value="CAZ80383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836192.1">
        <property type="nucleotide sequence ID" value="XM_002836146.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G790"/>
    <dbReference type="GeneID" id="9185281"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002516001"/>
    <dbReference type="HOGENOM" id="CLU_2185861_0_0_1"/>
    <dbReference type="InParanoid" id="D5G790"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ80383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="109" mass="12319" checksum="5C1E813F24D6E896" modified="2010-06-15" version="1">MIAYHIATAGESAETHHQPGRYHTPIPFLAAYRPRCHTTVLVSCMVIKGQVRYSTTLPKSRISLEKGWVDEGHGRLECVFVDGSWIYFSFLKEQENGLVGNLRGTQIRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7H0</accession>
    <name>D5G7H0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00002460001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430027">
        <property type="protein sequence ID" value="CAZ80463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836272.1">
        <property type="nucleotide sequence ID" value="XM_002836226.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7H0"/>
    <dbReference type="GeneID" id="9188488"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002460001"/>
    <dbReference type="HOGENOM" id="CLU_2777728_0_0_1"/>
    <dbReference type="InParanoid" id="D5G7H0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ80463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="69" mass="7750" checksum="4BF5CA691DF7408C" modified="2010-06-15" version="1">MVGISIIPYRQVLCLFFFSLSPSFPPSPSQQQSFPPPNWFLPQIYQLFSPIANSCSGIFLSVLLRNTSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>D5G843</accession>
    <name>D5G843_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00002806001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430039">
        <property type="protein sequence ID" value="CAZ80686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836495.1">
        <property type="nucleotide sequence ID" value="XM_002836449.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G843"/>
    <dbReference type="GeneID" id="9184159"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002806001"/>
    <dbReference type="eggNOG" id="ENOG502SASY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089396_0_0_1"/>
    <dbReference type="InParanoid" id="D5G843"/>
    <dbReference type="OMA" id="YNAKVYR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018858">
        <property type="entry name" value="DUF2458"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10454">
        <property type="entry name" value="DUF2458"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="234" mass="26484" checksum="CB080BC9D07BDDBD" modified="2010-06-15" version="1">MAVNEDLTRILQILSASTQATQAVPRPLPPVRSDEDDDSYSPPPAITPDPRQTYPPPSVHVSREAAVDPSKIEEWNAALRYIMKEVCKNEAIMAGIKKMINSQHEHEKAWHVALAKGQLLVFVLTVPRFRAREEIVNKHKVRKESGKKVEDVLRKLGTLPLAQQAIVQPTPESEAEELALFDRKVYTRSEQMVAHMTREFGSMGIPFFCHPSSASPEMLDRKRRMVELLQDLSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5G865</accession>
    <name>D5G865_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00002907001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the multicopper oxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430041">
        <property type="protein sequence ID" value="CAZ80708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836517.1">
        <property type="nucleotide sequence ID" value="XM_002836471.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G865"/>
    <dbReference type="STRING" id="656061.D5G865"/>
    <dbReference type="GeneID" id="9188310"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002907001"/>
    <dbReference type="eggNOG" id="KOG1263">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006504_7_1_1"/>
    <dbReference type="InParanoid" id="D5G865"/>
    <dbReference type="OMA" id="GFWLYHC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13857">
        <property type="entry name" value="CuRO_1_Diphenol_Ox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13886">
        <property type="entry name" value="CuRO_2_MCO_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13910">
        <property type="entry name" value="CuRO_3_MCO_like_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000045">
        <property type="entry name" value="Laccase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF414">
        <property type="entry name" value="ADR239WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0186">Copper</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Plastocyanin-like" evidence="8">
        <location>
            <begin position="101"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="6">
        <location>
            <begin position="224"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="7">
        <location>
            <begin position="444"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="599"/>
            <end position="624"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ80708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="624" mass="69656" checksum="6D60367A5154E566" modified="2010-06-15" version="1">MAVYWKLPTSEGENAAVASGTPQLTRLANVLRAISHTLGSISLAFVLSVSLLGGPAAFKTLRGPGFRVEKSFRRDPSDYVLDNDWDVYSTSTTRSYNWTISEIEAAPDGILRTLIVINGQFPGPLVECNEGDTIVIDVYNGATNSTSLHWHGQYQNGTNWMDGTAGVTNCPIPPGKSFRYEFTVREQWGTYWYHAHFSTSRIDGLFGPFIIHSPKESIQGDTDRIVMVHDYYHDLSAALLPQYLAPDSENDEPVPDGALINGMGICSTCEPSLGKLATFGLDRGKTHRLRFINVGAFAEFDVGIDQHELSLVEVDGVEVEPYSLHRFRINVAQRYSVILHANQPTNSYWLRARMIAHCFAEIPEDLRPEVLGIVRYTSNEEAPTTKGWGEAMELECKDLNTSEIVPAAAMSAPEPDVLMYLRSNFEIGAYRLSRGFFNKTSWRPAEVPTLLQAVGGLARVNISFPSEGVLPVYDINRQMVYRIDGIKTVDLLIDNFDDGNHPFHLHGYKFWVLGQGRGYFDKSTYAALNTTNPLRRDTVTIEAYGWVLIRFVADNPGMWAFHCHIAWHVEAGMLMQFLTQPDIVARWEAPEDVKGFCAHPEDQRGGGIPDEDFYEQARGPSGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8B9</accession>
    <name>D5G8B9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00004729001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430042">
        <property type="protein sequence ID" value="CAZ80762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836571.1">
        <property type="nucleotide sequence ID" value="XM_002836525.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8B9"/>
    <dbReference type="FunCoup" id="D5G8B9">
        <property type="interactions" value="1091"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G8B9"/>
    <dbReference type="GeneID" id="9188364"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004729001"/>
    <dbReference type="eggNOG" id="KOG2076">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002391_0_1_1"/>
    <dbReference type="InParanoid" id="D5G8B9"/>
    <dbReference type="OMA" id="SSPNMKF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000127">
        <property type="term" value="C:transcription factor TFIIIC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043035">
        <property type="term" value="F:chromatin insulator sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000995">
        <property type="term" value="F:RNA polymerase III general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039340">
        <property type="entry name" value="Tfc4/TFIIIC-102/Sfc4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23082:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR 3C POLYPEPTIDE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23082">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIIC TFIIIC , POLYPEPTIDE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="253"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="444"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="917"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ80762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="987" mass="113079" checksum="7867C1667B9D790B" modified="2010-06-15" version="1">MEHDGSEDDGFMSEEFRAEEFDEVYHDEDSDPDYAESDEDIDPALGALDDFTDDEGQSVRSDVDDENISLNALNDEMDTFHADLRAASGFKKHRRTGGRRQIGDEIYSFEIRGLLGQANSAYAFGKLDEAMKLVKQIIHIEPGVYSAWKILGEIFKEKGDKRKCLLAWLTAAHARPKDWELWVICAKMSLGQYGTDNRAYKDQAVYCYNRAISAKPDNIDAIYDRALLLKESGSLNKAAEGFVTLNRLLPNDMSVLKELAGLYIEMDKVPEAIEYYRRSVEYFKATGNPDNAFGWSELNIFVELYCLEKQWAKAIDTIGSVGRWLCGRAVETYWDKVNGDDREWDSDDRPRRCMVREFEVDRFQKESYCLPLELRVKLGLCRLRLGNMEEAMHHFQILHTEAQNSPSYSDLFQEVGDALYGGGHFNEAISYYSVVVEGAVYLDRKLWFNMADCYKGLDNIEDAEDCYGTVLEAYPRDDEAMMQLAGIYEVTGRKADALDLVNQIIGMRREKEKAEKAMKVVTATAQQTEEAESLAFFPNQPIPERVKRKRTGILGEAEKMEMNAKRTEQTEIRYRKLEYLRSSMEAGDPEAVKQWLDTAGDLVDDFRNTRALYPHERRNKFKGFMTTAQRRALALGETRRIEKMQDRLKQSLTFEEEEIEEPEHLSKFRGLDFDTWLYIFMQYAICLAKYDNYQDACDVCNAAKDANVFYHSKKRTFTIYITWLACAIHVGDSDSCSTLSRWFMTTFQFTTEAYNLFTASITASKNGLEVFHNNANQKYLLRQIKAMDQVLTGERKTGAANLTGECPSGGASGEKYVPKEADVGLIMLYAHILASGKSYLSALNYYTRAYALVPGDALITFCIGLAYLHRSMQRQSENRHIMVLQGVSFIFDYYDIRCGRPGDQEEAERDVRPEQRQEAEYNVGRAFHHIGLSHLAIPYYERVLQISEENTVDGDLKWEAAYNLQMIYMTSGNTKLAAEVTERYLVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8T7</accession>
    <name>D5G8T7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00004831001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sphingosine N-acyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430053">
        <property type="protein sequence ID" value="CAZ80930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836739.1">
        <property type="nucleotide sequence ID" value="XM_002836693.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8T7"/>
    <dbReference type="STRING" id="656061.D5G8T7"/>
    <dbReference type="GeneID" id="9186439"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004831001"/>
    <dbReference type="eggNOG" id="KOG1607">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="HOGENOM" id="CLU_028277_2_2_1"/>
    <dbReference type="InParanoid" id="D5G8T7"/>
    <dbReference type="OMA" id="KLYCLLQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
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    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0050291">
        <property type="term" value="F:sphingosine N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016439">
        <property type="entry name" value="Lag1/Lac1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006634">
        <property type="entry name" value="TLC-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12560:SF0">
        <property type="entry name" value="LD18904P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12560">
        <property type="entry name" value="LONGEVITY ASSURANCE FACTOR 1 LAG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03798">
        <property type="entry name" value="TRAM_LAG1_CLN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00724">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50922">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="134"/>
            <end position="154"/>
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    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="175"/>
            <end position="199"/>
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    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
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            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="244"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="293"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="352"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="TLC" evidence="9">
        <location>
            <begin position="169"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="386"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="391"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="422"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="PROSITE" id="PS50922"/>
        </source>
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        <source>
            <dbReference type="EMBL" id="CAZ80930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="452" mass="51670" checksum="D4BCC702D985FF2B" modified="2010-06-15" version="1">MYAPPSDDDRPSGRHHLSKGASRGRSGTDASKKGEKGSALRRKPSAAGTRGPVLKRRKSSPSWGIKKDLSMVDRIAAFFVENQIRFSAIILAIMHGCHYLVPQLRPTTRKLLEVCYYNPVTGNYGKGPDDVYLVFYWIVMFTFLRATAVDYIFMPFARRGGISAKKDLVRFAEQAWLLVYYSIFWTLGMYLMYNSPYWMDLAQMWVDWPVRELGGTFKWYYLVQYAFWLQQIFVLNIEERRKDYHQMFAHHIVTCMLIFASYTYHMTRVGNVILCVMDVVDILLPLAKMLKYLGYNAICDCAFGVFLITWFIGRHVCYMRIVHSSWVDALTHIREGWYAPNPMGTVVFTKEILGTFVILLLALQVLTLMWFYMILRVAWKVIKGGGADDTRSDDEDDGEEEEEEDIEFEGPNPKAVEAVQMNGASMSTASFPSVIMNGGSHRHLSSQPLGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>D5G996</accession>
    <name>D5G996_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00003208001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
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    <dbReference type="GO" id="GO:0005743">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:oligopeptide export from mitochondrion"/>
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    </dbReference>
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        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18578">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D2_like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolase protein"/>
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        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
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        <property type="entry name" value="AAA+_ATPase"/>
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        <property type="entry name" value="ABC1_TM_dom"/>
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        <property type="entry name" value="ABC1_TM_sf"/>
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        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
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        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF15">
        <property type="entry name" value="ALPHA-FACTOR-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="35"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="188"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="265"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="304"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="644"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="687"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="827"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="870"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="41"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="366"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="647"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="930"/>
            <end position="1155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ81089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1170" mass="128000" checksum="5975E9FB97A36BC0" modified="2010-06-15" version="1">MEAKKLTEGVSADGHGLAKQNSLSSLFAFVRKRNFVALLPALLFSIGSGLAIPTMSLLMGRIFSSFAGYSSNNIGSEELLASVSRYTSYLSLLGATRWLVSGSFFASWLVFGELQARVAHEELFRGIVGKDMEWFDTRKGIAELVSGCQTQINELKSAVSEPLGFTVQASVTASVSLGIALYHSWSLTLVILSGAPIALLLLSLLSIRMEPFIEAQNVHMSSSANTVSRSYTAIETVKAFNAQGLELRNFSAALGKAARAYRSQANLFALQVGCIRFITFAMFVQGFWYGGVLVRRGRADAGDVMTTFWSCLMATQSFEIILPQMMLLQKGMAAGGSLKGSLKQVERGRMPLKTGDIVPDILNGEIKFTNVSFTYPSRPSQLALNMVDFSIPAHRTTFIIGHSGSGKSTIGNLVLNMYQPVSGEITIDGLPLNSLDQEWLRENITVVQQPSVLFNETIFRNISVGRKDYRNVTEDQIRDACRMAMLEDTISELPLGYNTLAGVGGKQLSGGQRQRVALARAILRDTEILILDEATSNLDHKTGSSVMESIHEWRKGKTTIIITHDITQIKPENYVFILEDGRLVQSGVRSSIEDRHGLFQSFLKASSREANRGKEEISNDMELTELPSFQSPPSRNRSSSGYRILLIFGFIAAMFHGISTPLFSFFLARLLLSFFDTTNTPLESRKWSILVLAIAGLDGINSYIMHYLLESCGQAWIDYVRIRAFSRILCQPLTWFDGHEASSITEHLGKNAEEMRNLLGRFAGYAFVATVMMISGKGNDATSRIGNIFEETVSNIRTVRALSLEGYFGGKFLKATRAAVGIGIRRAIYAGIGFGVADSTILFATALIFYSGARLISTGHYTLEEIVTVFTLLLFCLANVNAIIGSTNLLVPQINNSKDTATRVLNLTTLSFGSHDEQNGTRAPDFTGPLVFRNVTFAYPSRPSLPVIYKLNLIIHPGECVAIVGHSGSGKSTLTSLLQRFYLPLHGTIFLSSDPLSSLDLPTLRSHISLENISYGGGCSRSEVESAARSAGIHEFIDSLRDGYNTCLVNGGSELSGGQAQMIAIARALVRPCSLLIFDECTSSLDSESARVVHETIKRLAEGERKMTVIIITHSKEMMRCADRIVVMSKGGVVEDGRFEELCTGEGELNRLLVRGESRRGNRGGGLDLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9E2</accession>
    <name>D5G9E2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00003267001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430059">
        <property type="protein sequence ID" value="CAZ81135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836944.1">
        <property type="nucleotide sequence ID" value="XM_002836898.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9E2"/>
    <dbReference type="FunCoup" id="D5G9E2">
        <property type="interactions" value="10"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G9E2"/>
    <dbReference type="GeneID" id="9182886"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003267001"/>
    <dbReference type="eggNOG" id="KOG1944">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049109_8_0_1"/>
    <dbReference type="InParanoid" id="D5G9E2"/>
    <dbReference type="OMA" id="FRVMPIQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF50">
        <property type="entry name" value="VACUOLAR MEMBRANE PROTEIN YOR292C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04117">
        <property type="entry name" value="Mpv17_PMP22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="134"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ81135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="228" mass="25462" checksum="5E49CC3EAC8A99C0" modified="2010-06-15" version="1">MASSDRLRLIRSARNYHLCGRVPLVNVVVLFIEIVGLARVAMQYDLHFGSKPILTMMITNSLLNGVADTIAQTVTSIRAKTRRPRDFGKKDSISIEIHELNEKGPLPTHRAELLATSPPPFDFERLVRYMAWGMFPYCASAIQVLILSPTSLAVFFIYMTLAEGGGKKAIIRKFDMVYISALKSNYILWPAVQILNFRVVPLSLQLPFASTVGIAWTVYLSLTNDAAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>D5GA00</accession>
    <name>D5GA00_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00003473001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430068">
        <property type="protein sequence ID" value="CAZ81343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837152.1">
        <property type="nucleotide sequence ID" value="XM_002837106.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GA00"/>
    <dbReference type="STRING" id="656061.D5GA00"/>
    <dbReference type="GeneID" id="9187041"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003473001"/>
    <dbReference type="HOGENOM" id="CLU_467840_0_0_1"/>
    <dbReference type="InParanoid" id="D5GA00"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031348">
        <property type="entry name" value="PigL_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17111">
        <property type="entry name" value="PigL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Azaphilone pigments biosynthesis cluster protein L N-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="345"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="416"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="305"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ81343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="583" mass="63402" checksum="CB8D1A6611EE975B" modified="2010-06-15" version="1">MDPLSVVSGIAGTVAVGTKVLVALHGFAKGVKSAPEEVKLLTRELENLCKIMDNFRKETKGLIVNQDIEDSSGAILDIFIQLNQLITEHTVSERDGLLKKGWKRVKWHYIEKDIADLRVHLNSNKGNLLVALTLGNELQNGHSNMKLERVQGALEEVIERLQDHDMQTVATCESFTLQRWQEVPSTIGPSSRPTTPNGQISVARSESGILSEAAFSERSIPMSAYQFNDMGNRGKGVLISNGTGTKAHTVKTGMLKKASNSKPSVERVTRAGPPASPPKLQIKSTKPTPLDIIPEPEDEDEAKNDSNPRAATAAQTFIKTPGKKRHLKPRGALGRARYRLRNFPKLRSGVDPGPARGGSGDGACTFVAPGTVDKPRDASAGVITKALPLRAPARAPPTAPGSPTLAMERFLQVLNTSPNLTSGDPPGGPGNIRKPSGGTYAIWPPYRPHVSLAGKVVSDAPIFLEVIDPPALSRDDSFQLQADKRVRLISYSNGEWFYGQVYPEGVVMQFRRAEFRHAPKPRDEEMRTEEKRQMLALLRPKKSTRIGSLEDWLGIGGNHTPKKTVGDVAISSPPALMEVVSAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAJ7</accession>
    <name>D5GAJ7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00003616001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430078">
        <property type="protein sequence ID" value="CAZ81540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837349.1">
        <property type="nucleotide sequence ID" value="XM_002837303.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAJ7"/>
    <dbReference type="STRING" id="656061.D5GAJ7"/>
    <dbReference type="GeneID" id="9187480"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003616001"/>
    <dbReference type="eggNOG" id="ENOG502RY8Z">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045137_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAJ7"/>
    <dbReference type="OMA" id="AWPTLLM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021836">
        <property type="entry name" value="DUF3429"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15887">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 69"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15887:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 69"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11911">
        <property type="entry name" value="DUF3429"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="155"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="209"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="240"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="290"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="339"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="339"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="379"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ81540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="430" mass="47420" checksum="633E2BD4633FB15E" modified="2010-06-15" version="1">MFRKTPTTNVTRSLFLPNARRSFLSRSPPLRTLPVVLGQKPPLGPLRAPMPRPLIAHVQPFTRLSPRWKQASKTNKVKSETAKREAELEKVLQEKLEAHPEEVTTTSSMTPIFGGQPKVPPRGEPPDETDMLRGLKSDFHAIKETFALKEVPKEVYYIGLGGTFPYAAISASTLYLAWDINYAHHNGVGYPVSAETAEQVLNFMEPLQVGLGAVILSFLGAVHWGLEMAGYGGKHGYKRYALSILAPALAWPTILLPFDYALITQFLGFTGMYFADSTATGWGWAPPWYMTYRFILTFVVGASIVLSLVGRGQIGDSVSKVPGPASYLQDLRDSQWENLQKEEDERRRKIRRQDAEERKKKAAKTKPKDDGIGGGAGESKVDKQAVDPAERTKGGEQREQGAYSDDKPGASSARGASSTELESTEPSKEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAN9</accession>
    <name>D5GAN9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00003707001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430083">
        <property type="protein sequence ID" value="CAZ81582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837391.1">
        <property type="nucleotide sequence ID" value="XM_002837345.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAN9"/>
    <dbReference type="GeneID" id="9187749"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003707001"/>
    <dbReference type="HOGENOM" id="CLU_1284102_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAN9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="215" mass="21727" checksum="9B39C758576DD106" modified="2010-06-15" version="1">MTKTAPIAAANVAGKRPDAAPGETMMDIGGAGPSGGVSCRRGGGGEGRVDQGGINGGDDELDDRNGGSLDLGGGGGGNDISGGGSGGDGGDRARDDSRDSLNDGGDGDVGRRGRESDGRWQAGDDGGVLRNVRLADTQEVFKRLAYVGIVLAPRLDALGNLGDEFCVRTEAVGVGGIVAVDDEDPSLEAARESGWALGWWKRGCGGGGGWWWVRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAP4</accession>
    <name>D5GAP4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00003716001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430083">
        <property type="protein sequence ID" value="CAZ81587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837396.1">
        <property type="nucleotide sequence ID" value="XM_002837350.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAP4"/>
    <dbReference type="STRING" id="656061.D5GAP4"/>
    <dbReference type="GeneID" id="9187754"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003716001"/>
    <dbReference type="eggNOG" id="ENOG502SNI2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092690_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAP4"/>
    <dbReference type="OMA" id="RQVHLMP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030151">
        <property type="term" value="F:molybdenum ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.33.20">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005302">
        <property type="entry name" value="MoCF_Sase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052716">
        <property type="entry name" value="MOSC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36930">
        <property type="entry name" value="METAL-SULFUR CLUSTER BIOSYNTHESIS PROTEINS YUAD-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36930:SF1">
        <property type="entry name" value="MOSC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03473">
        <property type="entry name" value="MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51340">
        <property type="entry name" value="MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MOSC" evidence="1">
        <location>
            <begin position="19"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="191" mass="20735" checksum="446EA14469E12332" modified="2010-06-15" version="1">MPPKIHSVSLSPTHSFSKRPVASITLVQGYGVSGDSHAGATIQHLSRQRTNPSAPNLRQVHFISREFLSTLSRDSEEPVNPGDLGENITTSDIDVLRLGRGDRLRFVKDGVEGEEGTVVVEITGLRNPCPQIERFRKGLQEKCVVRQKEGGRIVQRKAGVMGVVLLGGEVEAGMRILVEPAEGGRVPLEVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAU7</accession>
    <name>D5GAU7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00005313001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430086">
        <property type="protein sequence ID" value="CAZ81640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837449.1">
        <property type="nucleotide sequence ID" value="XM_002837403.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAU7"/>
    <dbReference type="FunCoup" id="D5GAU7">
        <property type="interactions" value="624"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GAU7"/>
    <dbReference type="GeneID" id="9187808"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005313001"/>
    <dbReference type="eggNOG" id="KOG2950">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024456_1_0_1"/>
    <dbReference type="InParanoid" id="D5GAU7"/>
    <dbReference type="OMA" id="GENTNFY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039905">
        <property type="entry name" value="CD2BP2/Lin1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003169">
        <property type="entry name" value="GYF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035445">
        <property type="entry name" value="GYF-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13138:SF3">
        <property type="entry name" value="CD2 ANTIGEN CYTOPLASMIC TAIL-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13138">
        <property type="entry name" value="PROTEIN LIN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02213">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55277">
        <property type="entry name" value="GYF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50829">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GYF" evidence="2">
        <location>
            <begin position="386"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="290"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="364"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="290"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="317"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ81640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="440" mass="50010" checksum="D571D1223712BD68" modified="2010-06-15" version="1">MFSSRPKPKRPLAISDAVRSGAGLPDSKRPKFDVRNPNELAPDAPDEEDVFLEIDEGAIGKRKSNRNKVDIEGYESDSSEEGFDATHKTWRKDEPKTDKMADEGDDMFADFKSDEEDYDNKEGDSGKGRKKDVKFMDLGQIEGQDFESKREFVDIIDEDKGRGEGEEDSDDQDEQEIDPEIGAGGKKKGAPKIDAFNMQNEMDEGRFDEAGNFIRKAAEKDAAHDSWLEGVSRKDMRRAKEAMEKREAEKRVKMKEDDAVPTSQVLADLIPCLEKGETILEALARLGTARKRKKNQINKNSWRQKKKAAANSEEMEVDKGKDPIDPIEARRKERVEQITGAADRLLTRGQPEIYDESRESLMRQYRQETGEDWAESRAPVTEDTTEKQWEFRWTDGRDGGDTHGPYDKSAMASWNQHGFFDQGAEFREVGGNWTLVSDFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GB36</accession>
    <name>D5GB36_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Endoplasmic reticulum-Golgi intermediate compartment protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00005433001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Plays a role in transport between endoplasmic reticulum and Golgi.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Endoplasmic reticulum membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Endoplasmic reticulum-Golgi intermediate compartment membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Golgi apparatus membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ERGIC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="FN430086">
        <property type="protein sequence ID" value="CAZ81729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837538.1">
        <property type="nucleotide sequence ID" value="XM_002837492.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GB36"/>
    <dbReference type="FunCoup" id="D5GB36">
        <property type="interactions" value="348"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GB36"/>
    <dbReference type="GeneID" id="9187970"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005433001"/>
    <dbReference type="eggNOG" id="KOG2667">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034705_2_0_1"/>
    <dbReference type="InParanoid" id="D5GB36"/>
    <dbReference type="OMA" id="MTNHYLR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033116">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061852">
        <property type="term" value="C:retrograde transporter complex, Golgi to ER"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045888">
        <property type="entry name" value="Erv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012936">
        <property type="entry name" value="Erv_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039542">
        <property type="entry name" value="Erv_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10984">
        <property type="entry name" value="ENDOPLASMIC RETICULUM-GOLGI INTERMEDIATE COMPARTMENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10984:SF81">
        <property type="entry name" value="ER-DERIVED VESICLES PROTEIN ERV41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07970">
        <property type="entry name" value="COPIIcoated_ERV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13850">
        <property type="entry name" value="ERGIC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="288"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="Endoplasmic reticulum vesicle transporter N-terminal" evidence="7">
        <location>
            <begin position="17"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="Endoplasmic reticulum vesicle transporter C-terminal" evidence="6">
        <location>
            <begin position="154"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU369013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ81729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="341" mass="37840" checksum="D4182707BF8ECD14" modified="2010-06-15" version="1">MNEEFFGEKRTSLGESVRTFDAFPKTRATYTTRTPRGGAITLLLLLTSACLTLTELRNYLTGSESHTFMVEPGIGHDMQINLDITVAMPCSSLHLNVQDAMQDRILAGELLSREEVWFDDAGAHKLYSEGADAETERRLFSSLVGKGAGEGGGSCRIYGSMGVNRILGDFHITAKGHGYWEDGAHIDHRSFNFSHVITELSFGDYYPKLVNPLDGVVSKTDENFHKFQYFLSIVPTTYESQTSGKSLLTNQYAVTEQSRKISSHSVPGIYFKYDIEPISLKISDRRTALLAFVVRLVNIVSGILVGGGWVYGLFGTLAGYFRRQRRTTDGMLNGRALGDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBC6</accession>
    <name>D5GBC6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00000423001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430091">
        <property type="protein sequence ID" value="CAZ81819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837628.1">
        <property type="nucleotide sequence ID" value="XM_002837582.1"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GBC6"/>
    <dbReference type="GeneID" id="9184392"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000423001"/>
    <dbReference type="eggNOG" id="ENOG502QSZW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_239006_0_0_1"/>
    <dbReference type="InParanoid" id="D5GBC6"/>
    <dbReference type="OMA" id="FMTPPHL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140445">
        <property type="term" value="C:chromosome, telomeric repeat region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022031">
        <property type="entry name" value="Rif1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22928">
        <property type="entry name" value="TELOMERE-ASSOCIATED PROTEIN RIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22928:SF3">
        <property type="entry name" value="TELOMERE-ASSOCIATED PROTEIN RIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12231">
        <property type="entry name" value="Rif1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0779">Telomere</keyword>
    <feature type="domain" description="Telomere-associated protein Rif1 N-terminal" evidence="8">
        <location>
            <begin position="151"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1105"/>
            <end position="1352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1365"/>
            <end position="1722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="63"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1192"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1242"/>
            <end position="1251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1369"/>
            <end position="1378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1393"/>
            <end position="1407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1425"/>
            <end position="1441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1480"/>
            <end position="1501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1506"/>
            <end position="1517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1546"/>
            <end position="1560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1644"/>
            <end position="1660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1663"/>
            <end position="1674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1705"/>
            <end position="1721"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ81819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1764" mass="195736" checksum="B3896569B166F035" modified="2010-06-15" version="1">MVQSETTSPYFAAAKSRSVEQMQPRPPTPPRESPRSASKTKRSVEKDCSIDSATESLVEPSSREITPPSSSGDNATVKPKKRVGWSPWTKYHKPDSLLSVTPIRSAIDPKSLKSILKPYSSPIPLFDDKMSSEQPIPESLPKLLDSIIQALESTERSKKIDAYVTFSNGMKAYDNILNLALMEEKLPSLCVFIKRDFDAKLDESGKSDSQLIQQAIKVLTILAWTLRLAESMDDGVASWFLQTAIDRVQDGHTPKLIVNHLLHFLAQQRFPQRIMTSERANQIITALDSLDQRVTGKSITKERIDIYFRLLSQARQTMLTRVNDWMDNLFGGLLSSVKEVRNRALACVAEIPRALGSEKSTARCLNNIFSRDTANGKRMFELIKERLELFIKDGEGVFVARMWGVVLLLIRVTAAEQWEFFTHWLRVIEGCFNVSDKDVKVEAQIAWQKLIYAMNIGPTTTRKLLELLCKPLGQYLDPKNALSNTKRPRMAAMVNISVLLYYGFRPNAPHKQLSEIWDVVVVGVLERLALSSKDEVEGGCKILSAVFEGSIQKPWSEDRVLKPPTMSPNELPRLDPKWVRSNSAIVLRTVETALRRTPWKTSETEDGPKLLWVRFVKAVSDAGSKEIKISGDLMDAVAQLLALFRRMWKVGPVAFNETQVGSSESFIRKFVFLINAAISALGTLPFTEKLLALDEENNFAPAATPPHKYSSGNSSMIIYSPVLHMISMFLLPTEGVVLDEEYHDCVKAILWRCCSSQDSRRKKLALIASCANLLPYKDADMLGLGMWQTLADLVALSLPIPTRERILLSPSPVGGEFRDAVKVLEWGCRYDLPGWESLLSRLCETVQNEQGDVHLAIGIIDPLAEIIRHQSVDYNKGLQLCKYLVDRVHYSKAKPHGDGASRTPLVVLGRKPNTTEPLEKLYDLIDHLLRKSYSSNVEGKALSETITLLDILGDLSSRCPPTSLVALLKRTQEGVGVWVLDLDHLIKTKQGEGVVQMLSAWTKITDAIVRLPQHDGKILADLATLIKCGLESRLKPIVNISVKSWNLTFGEQESIIYPKRVRGALKRLRPIADLKLPSFPDELEGDIIPTPPQFSESQEKFEELMQEKEVWMSPTPSRSGTSPFRRRSRASSPLTGQREGLFSSRPRRSLGTRNLQNPPARPKHTDSQIEFVAIETDPAEKGSSISSSQLTERQKEMKERQSETAALFGNLNAYSTSKRGRLPNDSAKATSSLRSKRLSRSPIKDSGRSRSGESLQTRFSPADLPESPLPKYTGSPMTQAPEAQSPKGPVVDITTNPPRAQDTGEMVDIIDDAINEAETRQGSSSPSIEGDDFVDAPENRISSGEEVEEVPKNSADIVVEISSVQGSRVCDERSHIESRFIPVSSEDEESSKINEPNTESDNPQSREPSPGVQIMTEERAASKLPAKRRRACSGPRRRSLARKASESPKKISGTITVAEPDAPHSKSPMSSYPAHEEQTESSPQKSQSTSISSPSKNSSSPGKLATISTMSPPTTRSSVKKAAIAKPPASTKSTRKRKLEAVDHYGSNNDENPSFENLATPTPKRRNTASLPVRRTRSSTKESFKSNDNGPDVSFIEETQLRVNSSIDDSQDHVDELVGPTPYVGSKAHTLFTEGTPKSSRGKGRLDSTARPETAEDTPKTPKTPRRLKRKSLGAHRQSQDFTPMVDSEKEADAGPSKGIGARRSLSTRQLETLSEPTSGETAIDGLQKALRALESASLTPDEYRWAENILFDAFSQLRRKGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBR9</accession>
    <name>D5GBR9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00005543001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ81919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837728.1">
        <property type="nucleotide sequence ID" value="XM_002837682.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBR9"/>
    <dbReference type="FunCoup" id="D5GBR9">
        <property type="interactions" value="418"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GBR9"/>
    <dbReference type="GeneID" id="9182956"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005543001"/>
    <dbReference type="eggNOG" id="KOG0712">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017633_0_2_1"/>
    <dbReference type="InParanoid" id="D5GBR9"/>
    <dbReference type="OMA" id="KWHEDGD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10747">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.230.10:FF:000001">
        <property type="entry name" value="DnaJ subfamily A member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.230.10">
        <property type="entry name" value="Heat shock protein DnaJ, cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044713">
        <property type="entry name" value="DNJA1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43888">
        <property type="entry name" value="DNAJ-LIKE-2, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00684">
        <property type="entry name" value="DnaJ_CXXCXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="DnaJ/Hsp40 cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072054" evidence="7">
        <location>
            <begin position="18"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="8">
        <location>
            <begin position="19"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="CR-type" evidence="9">
        <location>
            <begin position="143"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="6">
        <location>
            <begin position="143"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ81919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="404" mass="45935" checksum="1A368551A592AD7A" modified="2010-06-15" version="1">MRLFKALFLLLLPAVMAQDYYKLLDIDRKASDRDIKRAYRKLSKKYHPDKNPGDETAKQKFVEVAEAYEALADPESRQIYDQYGAEGLKQRQNGGGGGGHHDPFDLFSRFFGGGGHYHQGDRMRRGPNMEVKVHLPLRDFYNGAEKEFTVEKQMICEECEGTGSHDGHLESCNECGGRGVRVVKHMLAPGIFQQVQSVCERCGGKGKIISHPCKVCHGNKVVKKAATHSLNIERGSPRGIRISFENEADESPEWEAGDLIVHVDEKEADDNFEEEDLKHNYNGPPDGTWFRRRGKDLFWKEVLSLREALLGDWTRELVHLDGHKVKLTRAKGQTVQPGFVEIVPNEGMPMFRSEGGDQYGNLLVEYVVILPDQMESGMRKDINAVFEKWRKKKGVDLYVEKDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBT6</accession>
    <name>D5GBT6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00005572001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ81936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837745.1">
        <property type="nucleotide sequence ID" value="XM_002837699.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBT6"/>
    <dbReference type="GeneID" id="9182973"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005572001"/>
    <dbReference type="HOGENOM" id="CLU_1826695_0_0_1"/>
    <dbReference type="InParanoid" id="D5GBT6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="141" mass="15518" checksum="1AFF1CCF495AD51F" modified="2010-06-15" version="1">MYCAVKPFTSEWLPCYYLPPQSTSAPDNATPQSTKNNAHSPPNSREGIQRLSVLILANRLDMFTKLIATDHLNLQGADRENSTTLHEGRNAAIPFPETLLARRDNDINLYVAAAVSTDPAVIHLLLEGPRVDGPARSGNRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCT0</accession>
    <name>D5GCT0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00006004001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430120">
        <property type="protein sequence ID" value="CAZ82323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838132.1">
        <property type="nucleotide sequence ID" value="XM_002838086.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCT0"/>
    <dbReference type="FunCoup" id="D5GCT0">
        <property type="interactions" value="605"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GCT0"/>
    <dbReference type="GeneID" id="9187942"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006004001"/>
    <dbReference type="eggNOG" id="ENOG502SP5N">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024934_5_2_1"/>
    <dbReference type="InParanoid" id="D5GCT0"/>
    <dbReference type="OMA" id="KHAIECD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053157">
        <property type="entry name" value="Sterol_Uptake_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47784">
        <property type="entry name" value="STEROL UPTAKE CONTROL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47784:SF5">
        <property type="entry name" value="STEROL UPTAKE CONTROL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="3">
        <location>
            <begin position="13"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="55"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ82323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="413" mass="45757" checksum="D25B5CA64E886B49" modified="2010-06-15" version="1">MPPRRTHTKSRAGCAKCKKRRIKCDEVHPSCGNCVKHAIECDFSSVVGVPPAGVVSGAPSSSSDLPASNPMTLDASPPPGNGQHQVTTPHQQGIFPQSTVATVSPVINMTDMELIHHWSLSVPSSFTTTEDAESIWRDVIPRLAFRESQGCLVHAMLAISALHLAFIEPDKRSQRMCVAADHYGEAVRGVKDSVGAPAAIGKDNCEGLFVSSTLIVVYAIANPMLTEKVSPPTEIPPWTMPAWLPLIRGVHSILKTMWAWIEAGCLSPLLLGHTYIGDSAGEDEAMNALFRICTDKRELDADEIKDSAVSQAYFGAIHELQKNFANEVALRRKMVSLIFIWPISVSDAFFALLQERRPRALIIFAHYCVLLKKLESFWWVEGRANYELGRIERTLPEPWRKWLEWPVKMIRGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCY0</accession>
    <name>D5GCY0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00000863001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430128">
        <property type="protein sequence ID" value="CAZ82373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838182.1">
        <property type="nucleotide sequence ID" value="XM_002838136.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCY0"/>
    <dbReference type="GeneID" id="9183836"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000863001"/>
    <dbReference type="HOGENOM" id="CLU_3379616_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCY0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ82373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="34" mass="3987" checksum="9A9606F10BBC9EAC" modified="2010-06-15" version="1" fragment="single">INECINERNHKLITESCLEHNDKEVRAALVLAYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>D5GD38</accession>
    <name>D5GD38_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00000954001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430139">
        <property type="protein sequence ID" value="CAZ82431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838240.1">
        <property type="nucleotide sequence ID" value="XM_002838194.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GD38"/>
    <dbReference type="GeneID" id="9183900"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000954001"/>
    <dbReference type="eggNOG" id="KOG4476">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027883_2_0_1"/>
    <dbReference type="InParanoid" id="D5GD38"/>
    <dbReference type="OMA" id="FLCYREM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018608">
        <property type="entry name" value="Gti1/Pac2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28027:SF1">
        <property type="entry name" value="CAMP INDEPENDENT REGULATORY PROTEIN (AFU_ORTHOLOGUE AFUA_3G09640)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28027">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR MIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09729">
        <property type="entry name" value="Gti1_Pac2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="210"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="253"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="415" mass="45164" checksum="90A34E0146C0D71F" modified="2010-06-15" version="1">METYHGHVRSPADAIILFEACRLGLLPRVQRRLSEKERQNIKSGSVFVWDEREAGMRRWTDGKSWSASRVSGSFLTYREMEGKRGGNAFAPPTTTLSVGGRVGKSPNPDSDSDGLDTEGPDGYRYKPDGLMKQSFSITTATHQKLHLISYYARSHPSSQNLIQPTQDSALKHIRPAKGMYPESTVHEQQNVPVVTRGPMIATNFNHVQSPNAMIQRPPGATHPNSYPQQGAYGWPPSPVSTPPTNLPPPAGQYAQQLPLPNSAASSPMAYNPPTFPSAAGSPMQYDRTPLPHLDGLPPQQSHRGSMGSPMMHIHRSPRAAVQQMQRQPSDIPMPSLPCPDQGGRFSSPASSLNGRSPPRSEKFDINPLRQSPSGGMDGGSSPGRSGLEVQDIPSEKLGFGEDARALRVLNRAFIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GD92</accession>
    <name>D5GD92_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 13">Mannosyl-oligosaccharide glucosidase</fullName>
            <ecNumber evidence="11 13">3.2.1.106</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Glucosidase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">GSTUM_00006099001</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Cleaves the distal alpha 1,2-linked glucose residue from the Glc(3)Man(9)GlcNAc(2) oligosaccharide precursor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>N(4)-(alpha-D-Glc-(1-&gt;2)-alpha-D-Glc-(1-&gt;3)-alpha-D-Glc-(1-&gt;3)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] + H2O = N(4)-(alpha-D-Glc-(1-&gt;3)-alpha-D-Glc-(1-&gt;3)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] + beta-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:55988"/>
            <dbReference type="Rhea" id="RHEA-COMP:12806"/>
            <dbReference type="Rhea" id="RHEA-COMP:14355"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15903"/>
            <dbReference type="ChEBI" id="CHEBI:59082"/>
            <dbReference type="ChEBI" id="CHEBI:132537"/>
            <dbReference type="EC" id="3.2.1.106"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="14">Glycan metabolism; N-glycan degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Endoplasmic reticulum membrane</location>
            <topology evidence="1 13">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the glycosyl hydrolase 63 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.106" evidence="11 13"/>
    <dbReference type="EMBL" id="FN430142">
        <property type="protein sequence ID" value="CAZ82485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838294.1">
        <property type="nucleotide sequence ID" value="XM_002838248.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GD92"/>
    <dbReference type="FunCoup" id="D5GD92">
        <property type="interactions" value="564"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GD92"/>
    <dbReference type="GeneID" id="9183949"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006099001"/>
    <dbReference type="eggNOG" id="KOG2161">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007380_1_0_1"/>
    <dbReference type="InParanoid" id="D5GD92"/>
    <dbReference type="OMA" id="FNWYNTT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098553">
        <property type="term" value="C:lumenal side of endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004573">
        <property type="term" value="F:Glc3Man9GlcNAc2 oligosaccharide glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070880">
        <property type="term" value="P:fungal-type cell wall beta-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006491">
        <property type="term" value="P:N-glycan processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009311">
        <property type="term" value="P:oligosaccharide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000027">
        <property type="entry name" value="Probable mannosyl-oligosaccharide glucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.110">
        <property type="entry name" value="Glycosyl hydrolase family 63, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031335">
        <property type="entry name" value="Glyco_hydro_63_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031631">
        <property type="entry name" value="Glyco_hydro_63N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038518">
        <property type="entry name" value="Glyco_hydro_63N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004888">
        <property type="entry name" value="Glycoside_hydrolase_63"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10412">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10412:SF11">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03200">
        <property type="entry name" value="Glyco_hydro_63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16923">
        <property type="entry name" value="Glyco_hydro_63N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 13" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072044" description="Mannosyl-oligosaccharide glucosidase" evidence="15">
        <location>
            <begin position="23"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 63 N-terminal" evidence="17">
        <location>
            <begin position="43"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 63 C-terminal" evidence="16">
        <location>
            <begin position="329"/>
            <end position="832"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00052431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU368089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU369107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF03200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF16923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAZ82485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="847" mass="96524" checksum="053081B7882C12A3" modified="2010-06-15" version="1">MAKLTLSLLAVCSALLAPGTTAANTDNGQWILANEISRVSNQSLVWGPYRPNLYFGVKPRIPKSMIAGLMWSKVDDFASFAQTVRHTCEQSDDMAGYGWDEYDVRTGGHQVIKDPGNSINIHTEFIKIEGGENGEIGPIKTGVGLIIILRTLGGHWGVRVKGIPRPGAPVDLKTMVIFYVGLEGLGSLSLQNEPDERGLAGDVTLEGSMPELGEFKIDITTGPGTNKPPQVGHKKYWKEKPLDRTLYRSLKVPEEDVWKAKDTFFMGLRDNFLKAKEKYGESDLPPPWQVFTIPNTFGPGNFHMVQKVFEGGFEFDILYSSASAPTSMTTDILTEKIRTVGKSFADRFENIFPREPPFNAPRYRQFARNFFSNLLGGIGHFYGTSVIDRTYAPEYEEEDEGFWEEAKAAQARPDAANLEGPTELFTSIPSRPFFPRGFLWDEGFHLLPISDWDMDLTLQIVKSWVSLVDRDGWLPREQILGDEARSKVPQEFQTQYPHYANPPTLFFVLTKFIDHSNHRSLANEYLDKESFDNLFSSGDLPTVHLENPKAASEYLRSIYPNLRRNYFWYRKSQSGEIRGWDRNAYSKKEGYRWRGRTPEHCLTSGLDDYPRARPPHTGELHVDLLSWIGMMTSCIRRIAAAIGEHDDVAEFEQYEYSIKKNLDDLHWNEKHQTYCDATIDDFEESVHVCHKGYISIFPFLLGLMEKDSKKLGKVLDLIADPEELWSNYGLRSLSKKDEYYGTGENYWRGPIWININYLAIQRLMEYGSKEGPHQKQAAELYKELRVNVVENVYKSWKDTGYAWEQYDAETGKGQRTAHFLGWTSLVVKIMNMPEAVSVVATPLKDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDG1</accession>
    <name>D5GDG1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidyl-prolyl cis-trans isomerase</fullName>
            <ecNumber evidence="5">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00000996001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="5"/>
    <dbReference type="EMBL" id="FN430143">
        <property type="protein sequence ID" value="CAZ82553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838362.1">
        <property type="nucleotide sequence ID" value="XM_002838316.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDG1"/>
    <dbReference type="FunCoup" id="D5GDG1">
        <property type="interactions" value="930"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GDG1"/>
    <dbReference type="GeneID" id="9184018"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000996001"/>
    <dbReference type="eggNOG" id="KOG3259">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090028_0_0_1"/>
    <dbReference type="InParanoid" id="D5GDG1"/>
    <dbReference type="OMA" id="DEVQCLH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140463">
        <property type="term" value="F:chromatin-protein adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0180010">
        <property type="term" value="P:co-transcriptional mRNA 3'-end processing, cleavage and polyadenylation pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060261">
        <property type="term" value="P:positive regulation of transcription initiation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.40:FF:000026">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051370">
        <property type="entry name" value="PPIase_Pin1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000297">
        <property type="entry name" value="PPIase_PpiC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10657">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10657:SF4">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00639">
        <property type="entry name" value="Rotamase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50198">
        <property type="entry name" value="PPIC_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4 5" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="WW" evidence="6">
        <location>
            <begin position="22"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="PpiC" evidence="7">
        <location>
            <begin position="84"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ82553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="195" mass="21634" checksum="455ECC5ECADA823B" modified="2010-06-15" version="1">MNGIAYCTCSLSNNQSVMTSETGLPENWTIRLSKSKGLPYYFNTTTTESRWEPPSDTDTDKLKVYMAEKFSTGNTAPPEGSGQAGKIRCAHLLVKHKDSRRPSSWKESNITRTKEDAMGIILGFETRIRDRSTSLADLAVVESDCSSARKRGDLGFFGRGEMQKEFEEAAFALRPGDMSHAVETASGIHLIERLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDJ0</accession>
    <name>D5GDJ0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Autophagy-related protein 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00000963001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in cytoplasm to vacuole transport (Cvt), pexophagy, mitophagy and nucleophagy. Recruits mitochondria for their selective degradation via autophagy (mitophagy) during starvation. Works as scaffold proteins that recruit ATG proteins to the pre-autophagosome (PAS), the site of vesicle/autophagosome formation. Required for the Cvt vesicles completion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Preautophagosomal structure membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Vacuole membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
        </subcellularLocation>
        <text evidence="7">During pexophagy, accumulates in the vacuolar membrane region, where the peroxisomes contact the vacuole.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the ATG11 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430146">
        <property type="protein sequence ID" value="CAZ82583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838392.1">
        <property type="nucleotide sequence ID" value="XM_002838346.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5GDJ0">
        <property type="interactions" value="136"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GDJ0"/>
    <dbReference type="GeneID" id="9188176"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000963001"/>
    <dbReference type="eggNOG" id="ENOG502QUQE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002803_1_0_1"/>
    <dbReference type="InParanoid" id="D5GDJ0"/>
    <dbReference type="OMA" id="GLRWYLI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990316">
        <property type="term" value="C:Atg1/ULK1 kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903599">
        <property type="term" value="P:positive regulation of autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034517">
        <property type="term" value="P:ribophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040040">
        <property type="entry name" value="ATG11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019460">
        <property type="entry name" value="Atg11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045326">
        <property type="entry name" value="ATG17-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13222">
        <property type="entry name" value="RB1-INDUCIBLE COILED-COIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13222:SF1">
        <property type="entry name" value="RB1-INDUCIBLE COILED-COIL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10377">
        <property type="entry name" value="ATG11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04108">
        <property type="entry name" value="ATG17_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0072">Autophagy</keyword>
    <keyword evidence="6 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0926">Vacuole</keyword>
    <feature type="domain" description="Autophagy protein ATG17-like" evidence="10">
        <location>
            <begin position="100"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="Autophagy-related protein 11 C-terminal" evidence="11">
        <location>
            <begin position="1010"/>
            <end position="1162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="540"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1118"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1202"/>
            <end position="1224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1275"/>
            <end position="1294"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="501"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="657"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="752"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="856"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="86"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="552"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1118"/>
            <end position="1140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1214"/>
            <end position="1224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF10377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ82583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1294" mass="143945" checksum="0C9349A514245F50" modified="2010-06-15" version="1">MSLLIYTAHTGGTLQADRHSFTSVDELRKWIGETISMAPACQIIMTARGTNVKNQSLPNEKELYLFDRRLLDASSSPPTELIPRLQTPSPFPTNLESETELSSWRTLFRKRLAWSESVLSHAQNLAATISKTDASTNVVQLSVQIAFSNLEVHSLGLQNSLVKLQEWAEGVLVQLDRVFQEWEPALKRLVRIPVHDEVKKYGQNGSGKERKVSTLMDFFEVKQVQTAAVAAKGLAQQFEKEVTDLGGTIEEICARTADLKSDIQKARWVWLVGWRCQSYYEYVQSLQGPKSVSTASKRAYASTTDYLPGLANIVNDMGRLLQIAVKQKNEISATSVRQLQIVAFVQSLSAPVNPQIQKMDSAYQEEDEHPRLLSLVVRFPKIYGALLVECVRRREWSEKFANDSKRIAEEFALLKEDEEKRRARNKIPSPTGGVTEKFQDRTVESFLSVVQNIEGLDSVLKEVRQLYQDLDRPLGRRSKRLKGFKFGSIHEASLGASSIFGRSEEEEVKILREDKQNLTERVKGYESRIRKLEDLLHRGRTSGGNMYQVAGSSTPQQPSTPQPNQTSFPTSIAGPLLSSDRPSSPAAIRRISVENADKPMVARISALEAELAAEKELTAQLQRDASSRIDLEKAMTTRMALADETKRDLVANLEDMGQQHIVERRVMEQEIEELRQKLDEAYEEMDRIEEGRNEEARRRSVAEERMKGLEGELAIMGEEVDRLRMLNEDQVKRVKELVDKVEFTRSASDHDLEGLRGELKAQKKLVEAEEEKTRDVEAALDESQGKNRELVGAIETFEAQIKQMKDSLDTLNAQYTAQQQGQESIAAAIRHVHSQLSTDPPPDNIDTVQATVQALISKALERINDLDAKVKEEEARASEVEARKALLQSRFDSRTIKAKDLTQRLYTHNARSIQLLESLGYRIVRGEDSMQIVKVPKSANTESIVLSRSAHNMEASVTGKPSPLSLAHSPTVEDINLLYWMETEDSDAETEKYTQYLKKIGAFDLDAFSDIVTSRVKKLEQDCRQLVKQCRSYREKYYRSRDEANEKIAYKSFKPGDLALFLPTRNSVTRPWAAFNVNAPHFFLKEDSSHKLANRDWLLARISKIEDRVVDLSRSTATLTLPPSASSPSSSSTDKPFSAAALDDENPFELSDGLRWYLLEAIEEKPGAPTTPGLSTSTVAAANVDAKGSLRARKGVTGAKKTLTQITTEHNSRRSSSGSNSHRNSLVVGPEVAAGLRDAVVNGGVAASAVESVPGTPITGVGEKGMEALRGIAAAASAADGNGGNDAPLGGTRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDM8</accession>
    <name>D5GDM8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00001124001</name>
    </gene>
    <organism evidence="5 6">
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        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
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            <taxon>Tuber</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5 6">Mel28</strain>
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    </evidence>
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</entry>
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    <accession>D5GDN9</accession>
    <name>D5GDN9_TUBMM</name>
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        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
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    </gene>
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        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
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            <taxon>Ascomycota</taxon>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
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                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
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                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="67" mass="7549" checksum="30B8C86D29268388" modified="2010-06-15" version="1">MVFWTLSQELDPVCRALAQYSNHLHAAQCAQQLRDVLLVRYAIIPCPSALSSINVYSWCSARISPIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDP2</accession>
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    <protein>
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        </alternativeName>
    </protein>
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        <name evidence="25" type="ORF">GSTUM_00006208001</name>
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        <lineage>
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        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25 26">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="21">ATP-dependent DNA helicase important for chromosome transmission and normal cell cycle progression in G(2)/M. May have a role in changing DNA topology to allow the loading of proteins involved in maintaining sister chromatid cohesion in the vicinity of the centromeres. Has a specific role in chromosome segregation during meiosis II.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DEAD box helicase family. DEAH subfamily. DDX11/CHL1 sub-subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.3" evidence="18"/>
    <dbReference type="EMBL" id="FN430153">
        <property type="protein sequence ID" value="CAZ82635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838444.1">
        <property type="nucleotide sequence ID" value="XM_002838398.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDP2"/>
    <dbReference type="FunCoup" id="D5GDP2">
        <property type="interactions" value="929"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GDP2"/>
    <dbReference type="GeneID" id="9181640"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006208001"/>
    <dbReference type="eggNOG" id="KOG1133">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006515_2_0_1"/>
    <dbReference type="InParanoid" id="D5GDP2"/>
    <dbReference type="OMA" id="QTHQFRD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045005">
        <property type="term" value="P:DNA-templated DNA replication maintenance of fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034085">
        <property type="term" value="P:establishment of sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031571">
        <property type="term" value="P:mitotic G1 DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18788">
        <property type="entry name" value="SF2_C_XPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001372">
        <property type="entry name" value="ATP-dependent DNA helicase chl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002774">
        <property type="entry name" value="ATP-dependent DNA helicase chl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006555">
        <property type="entry name" value="ATP-dep_Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045028">
        <property type="entry name" value="DinG/Rad3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014013">
        <property type="entry name" value="Helic_SF1/SF2_ATP-bd_DinG/Rad3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006554">
        <property type="entry name" value="Helicase-like_DEXD_c2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010614">
        <property type="entry name" value="RAD3-like_helicase_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013020">
        <property type="entry name" value="Rad3/Chl1-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00604">
        <property type="entry name" value="rad3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472:SF41">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE DDX11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472">
        <property type="entry name" value="DNA REPAIR DEAD HELICASE RAD3/XP-D SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06733">
        <property type="entry name" value="DEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13307">
        <property type="entry name" value="Helicase_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00488">
        <property type="entry name" value="DEXDc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00491">
        <property type="entry name" value="HELICc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51193">
        <property type="entry name" value="HELICASE_ATP_BIND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="23" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0347">Helicase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="14" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="24">
        <location>
            <begin position="1"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="23">
        <location>
            <begin position="71"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00029709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00044969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00044998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00045008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00045702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00048954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="CAZ82635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="807" mass="90469" checksum="BD349A4C6D59AA9F" modified="2010-06-15" version="1">MPFTQLLRMDVLVFSNRQLVEFFGKSLSLICGALTWLREHKSKSIESQLKEDGEDDGAPDWVKQFARDEKKQNLLQEKADMEARLADIREKEKREKQLANSSSNLKIKRRYIAVWLTGEQKGEIEVEVDGNGDEAQFCLEDYESGDEDGGAQGSLGGDALSPAIFYCSRTHSQLTQFINELRRVKFPPSSPGGEGSALEEEVKHLSLGSRKNLCINPKVTKLGNPTAINERCLELQRSGASEASRCEFLPGRDDQVAVRDFRDHTLAKIRDIEDLASLGRKMGICPYYASRPTIKPSEIVTLPYPLLLQKSAREALDLSLKGHIVIVDEAHNLINAISSIYSITVSLAQLQRSKSQLDIYLARFKNRLKGKNKVYVMQTLRIIASLASYLEEISKKSKEGVIAPGDLLAMQGIDQVNFYKLQTYLNESKLARKVEGYIVHKQAKEQEKNKLPNKPGGAKALQPTASIPVLTHIQGFLLALTNPSAEGKLFHGKTEDKNDTCLRYMLLDPAHHFQEIVEEARAVILAGGTMEPMNDYTNHLFPYLPKEKIRTLSCGHVIPKENLTALPVTKGPGGREFEFTFEKRMQPAMIEELGRAIVNLCQVIPHGVVCFFPSYAYLEFVVSQWRKKPTPASNTAAKSLWERLEDRKTVFRESSDGSSVEEVLREYAQAIDSGKGGLLLSVVGGKMSEGINFNDNLGRGIIMVGLPFPNSNTAEWRAKLEHAEKAAGREFYENACMRAVNQSIGRAIRHREDYAVIALFDRRYSTDRIASKLPKWIQNGLVRGPVDKVFGEVMVITSRFFKTRDAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDS9</accession>
    <name>D5GDS9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00006257001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the actin family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430153">
        <property type="protein sequence ID" value="CAZ82672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838481.1">
        <property type="nucleotide sequence ID" value="XM_002838435.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDS9"/>
    <dbReference type="FunCoup" id="D5GDS9">
        <property type="interactions" value="708"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GDS9"/>
    <dbReference type="GeneID" id="9184467"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006257001"/>
    <dbReference type="eggNOG" id="KOG0678">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027965_3_0_1"/>
    <dbReference type="InParanoid" id="D5GDS9"/>
    <dbReference type="OMA" id="GIHYPIR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051285">
        <property type="term" value="C:cell cortex of cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044396">
        <property type="term" value="P:actin cortical patch organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030041">
        <property type="term" value="P:actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd10221">
        <property type="entry name" value="ASKHA_NBD_Arp3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000029">
        <property type="entry name" value="Actin-related protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="41"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ82672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="421" mass="46024" checksum="7D0D54C627AF5343" modified="2010-06-15" version="1">MASQTPAVVMDNGTGYSKLGFAGNDSPSFVFPTAIAIKGSSGGGGGSGSGRPAIAQKPSYLTGGAGPSGHLSAKRGTEDLDYFIGDEALAQASGPGYGIHYPIRHGQVDNWDHMERFWQNSIFKYLRVEPEDHYFLLTEPPLNPPESRENTAEIMFETFNCAGLYIAVQAVLALAASWTSSKVQDRSLTGTVVDSGDGVTHVIPVAEGYVIGSSIKSIPIAGRDITFFVQSLLRDRNEPDSSLKTAEQIKEEFCYRFQKYVAEQLGGRKVTVDVGYERFLAPEIFFNPEIYSSDFLTPLPTVVDTVIQSSPIDVRRGLYKNIVLSGGSTLYKDFGRRLQRDIRHLVDARIRKSEEKSGGVKSGGLEVQVITHKRQRHGPWFGGSLLGQTPEFRSYCHTKAEYDEIGPSIVRRFALLGGPGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>D5GE30</accession>
    <name>D5GE30_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Crossover junction endonuclease MUS81</fullName>
            <ecNumber evidence="14">3.1.22.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">GSTUM_00006347001</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Interacts with EME1 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, D-loops, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication fork intermediates. May be required in meiosis for the repair of meiosis-specific double strand breaks subsequent to single-end invasion (SEI).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="14">Interacts with EME1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the XPF family.</text>
    </comment>
    <dbReference type="EC" id="3.1.22.-" evidence="14"/>
    <dbReference type="EMBL" id="FN430164">
        <property type="protein sequence ID" value="CAZ82773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838582.1">
        <property type="nucleotide sequence ID" value="XM_002838536.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GE30"/>
    <dbReference type="FunCoup" id="D5GE30">
        <property type="interactions" value="172"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GE30"/>
    <dbReference type="GeneID" id="9183105"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006347001"/>
    <dbReference type="eggNOG" id="KOG2379">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014329_1_0_1"/>
    <dbReference type="InParanoid" id="D5GE30"/>
    <dbReference type="OMA" id="PGMLQMP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048476">
        <property type="term" value="C:Holliday junction resolvase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048257">
        <property type="term" value="F:3'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008821">
        <property type="term" value="F:crossover junction DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006308">
        <property type="term" value="P:DNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd21036">
        <property type="entry name" value="WH_MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20074">
        <property type="entry name" value="XPF_nuclease_Mus81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000307">
        <property type="entry name" value="Crossover junction endonuclease MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10130:FF:000003">
        <property type="entry name" value="Crossover junction endonuclease MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.110:FF:000001">
        <property type="entry name" value="Putative Crossover junction endonuclease MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.670">
        <property type="entry name" value="Crossover junction endonuclease EME1, DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.110">
        <property type="entry name" value="DNA polymerase beta, N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027421">
        <property type="entry name" value="DNA_pol_lamdba_lyase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042530">
        <property type="entry name" value="EME1/EME2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006166">
        <property type="entry name" value="ERCC4_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010996">
        <property type="entry name" value="HHH_MUS81"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033309">
        <property type="entry name" value="Mus81"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047417">
        <property type="entry name" value="WHD_MUS81"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047416">
        <property type="entry name" value="XPF_nuclease_Mus81"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13451">
        <property type="entry name" value="CLASS II CROSSOVER JUNCTION ENDONUCLEASE MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13451:SF0">
        <property type="entry name" value="CROSSOVER JUNCTION ENDONUCLEASE MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21292">
        <property type="entry name" value="EME1-MUS81_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02732">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14716">
        <property type="entry name" value="HHH_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21136">
        <property type="entry name" value="WHD_MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00891">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47802">
        <property type="entry name" value="DNA polymerase beta, N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10 14" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="11 14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6 14" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="8 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0469">Meiosis</keyword>
    <keyword evidence="5 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 14" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ERCC4" evidence="16">
        <location>
            <begin position="455"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="82"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="226"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="376"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="284"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="317"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="346"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="376"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="398"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="421"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU369042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAZ82773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="748" mass="82161" checksum="73310736888905FB" modified="2010-06-15" version="1">MADDECANPLLLGFLRDWYDTARQQNSKRVTTYRNAYEAMRVCPIPFQHPSEAIQLKGLGPVLCERLTQALRIHCEANGLPMPKKAKRRRARDDLDGGESAAGEGSGAAPPPKKRRPTKAYIPKKRTGAYALLYALYKGDGDMLTKTELIDRARPYCDSSFDAPAEAGKFYTAWASMRTLISSNYVYFSGNPKRYMLTEGGEEVGRGILAAEEGLDLGLGDTDGGFGEAAQVGGPTARGGATASGSVPSSRSKRTPAVRNTWGGDGSEDSSDVAPSRHRRRADPRHEEDNRPILRRILEEPEANSRASEDGLELSDCASTLGRNSMPLSQNTGPINDGRRLPVVPSGSQTPSVAPLPRTSTEIDLTLSPEHPSATVLTQKSKSNANIPAVPHRHKIGNVTSQASSITSVDRGAPTSSRPQSRAGSTTASRAASGSRSTFPDFSPEIVKAGSFTVHLLLDNREVRTKNDRDYIQDNLKTCGINPITRPLQVGDAMWIACERGGRNREIALDHIAERKRLDDLVASIKDGRFHEQKFRLAKSGLKHVIYIIEDFTLKEADRAMQDAMNTAISSTQVVDGFFVKRTGKLDDTIRYLVRMTKKLEKEYETKDLSLIPDNLLDSSTYQAFRKHLNEKFPEKQFNINYSIFSTLVSKSASLNLRDVYLKMLMCVRGISLDKALQIQKAYPTPIELVEAYERCASEDEMKEMIMKQSASALGRKKVGPAVSAKVAEVWTGVGAQADPDEEAEDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5GE75</accession>
    <name>D5GE75_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00006410001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430164">
        <property type="protein sequence ID" value="CAZ82818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838627.1">
        <property type="nucleotide sequence ID" value="XM_002838581.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GE75"/>
    <dbReference type="STRING" id="656061.D5GE75"/>
    <dbReference type="GeneID" id="9185474"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006410001"/>
    <dbReference type="eggNOG" id="KOG0866">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019573_1_0_1"/>
    <dbReference type="InParanoid" id="D5GE75"/>
    <dbReference type="OMA" id="ILREMEF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000156">
        <property type="entry name" value="Ran_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045255">
        <property type="entry name" value="RanBP1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138">
        <property type="entry name" value="RAN BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138:SF142">
        <property type="entry name" value="RAN-BINDING PROTEIN 3B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00638">
        <property type="entry name" value="Ran_BP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00160">
        <property type="entry name" value="RanBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50196">
        <property type="entry name" value="RANBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RanBD1" evidence="4">
        <location>
            <begin position="424"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="26"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="93"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="159"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="217"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="285"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="299"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="343"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="354"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="388"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="416"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ82818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="570" mass="60736" checksum="6C799DEF082A7FB4" modified="2010-06-15" version="1">MTNSGVATDDPGAKSPSKTPLQLIARENDKDDNSVIHGHDMDSLEPTHKESEVADDEDSGSLSGSSDNEQVREKLKKTYIANVRPESAAASESQKEDGESKATDSAAVSETDQDMGEEKPRRPRKRSIEETDPGEDVNMGNEGNGKETDSRHRVHERKRSREISDDDMAKAARALNRVKTPPTHPEEDEIMGHNDGLHSASSAMTSPRKLERKRSRNRFDEDDKDKDADRRKKMVAERKEETDSGMGADIPTPSNELTNGKAFGKPEVARMSSTETDTKSESKTLASSGFGNTSAQSPFATASSAFGAMSGSAASPFATLGSASHPPSPFGALGNTTAKPPAFSSGFGSSYGPSGFGSLGGKAGDVISSAPKLSGPIGGTAAKPFGAPDDDDEKTDDENGEGGGVDLEAHKEASSGKETFTQQEIATGEEGEITVFKCPGKIFSFDKENKVWKERGKGTFKLNTKGSKSSISASAFGLDDTPSASVPEEKKSARILMRTDGTYTLILNVPIFKGMIFGDHAGNKPTGNQFLLLIPNSSGGLDSMTLKLKSAAHAKELYDHVRNLHDELFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5GED7</accession>
    <name>D5GED7_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 15">Chromatin-remodeling ATPase INO80</fullName>
            <ecNumber evidence="15">3.6.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">GSTUM_00006436001</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">ATPase component of the INO80 complex which remodels chromatin by shifting nucleosomes and is involved in DNA repair.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Component of the INO80 chromatin-remodeling complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="15">The DBINO region is involved in binding to DNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.-" evidence="15"/>
    <dbReference type="EMBL" id="FN430175">
        <property type="protein sequence ID" value="CAZ82880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838689.1">
        <property type="nucleotide sequence ID" value="XM_002838643.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GED7"/>
    <dbReference type="STRING" id="656061.D5GED7"/>
    <dbReference type="GeneID" id="9185536"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006436001"/>
    <dbReference type="eggNOG" id="KOG0388">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_826415_0_0_1"/>
    <dbReference type="InParanoid" id="D5GED7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001269">
        <property type="entry name" value="SNF2 family helicase/ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050520">
        <property type="entry name" value="INO80/SWR1_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45685:SF2">
        <property type="entry name" value="CHROMATIN-REMODELING ATPASE INO80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45685">
        <property type="entry name" value="HELICASE SRCAP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0010">Activator</keyword>
    <keyword evidence="7 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 15" id="KW-0227">DNA damage</keyword>
    <keyword evidence="13 15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10 15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Helicase C-terminal" evidence="17">
        <location>
            <begin position="127"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="311"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="311"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="322"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="347"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="362"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="378"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU368001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAZ82880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="404" mass="45424" checksum="16A5585E0BF0189D" modified="2010-06-15" version="1">MLRRIKKHVQKELGDKIELDVQCELTYRQRALYKGLRDKISIMDIIEKAASGADENNATLMNLVMQFRKVCNHPDLFERAETSSPIDLGLYPIPKILPEPSSEKMGYAHIKVPSMRRFVSDSGKLAKLDALLTELKAGGHRVLLYFQMTKMMDLCEEYLTYRHHRYLRLDGSSKLEDRRDMVSAWQTTPEIFVFILSTRAGGLGINLTAADTVIFYDSDWNPTIDSQAMDRAHRLGQTRQVTVYRLITRGTIEERIRTRAKQKEEVQRVVIQGGDPGAKKNTVDFKGTREVATWLFDDEEAEQLEKTLEAKEKEAEEAKAKGGAKRGKKKAKTGPAGEEPKAINLEDLYHEGEGRFDDSGKPSEAGTPAASAAPVPSSRKRGPKGGNKKKTTQERLAMIDGVTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEQ0</accession>
    <name>D5GEQ0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00001324001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430183">
        <property type="protein sequence ID" value="CAZ82993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838802.1">
        <property type="nucleotide sequence ID" value="XM_002838756.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEQ0"/>
    <dbReference type="FunCoup" id="D5GEQ0">
        <property type="interactions" value="237"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GEQ0"/>
    <dbReference type="GeneID" id="9184904"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001324001"/>
    <dbReference type="eggNOG" id="KOG3043">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054590_2_1_1"/>
    <dbReference type="InParanoid" id="D5GEQ0"/>
    <dbReference type="OMA" id="CRYLKAG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002925">
        <property type="entry name" value="Dienelactn_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17630">
        <property type="entry name" value="DIENELACTONE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17630:SF44">
        <property type="entry name" value="PROTEIN AIM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01738">
        <property type="entry name" value="DLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dienelactone hydrolase" evidence="1">
        <location>
            <begin position="31"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="244" mass="26838" checksum="B19537830861D1F2" modified="2010-06-15" version="1">MASNTPGECCMKGVMHEGTPKGEMVQFAEVETYITKPASGSSEKVILLLTDVMGICNNPKLIADNFAANGYTVVLPDIFAGDKVPLPADRKPGFQLPEWLKNHQPHHVVPIIQEVMGQIRAELKPRKIGAAGYCFGAKYVTQLLSGEIDAGYNAHPSFVTLDELCAIKAPLAISAAETDSIFTPDLRHATEAKLAEIKATYQITLFSGVEHGFAVRCDLANEKQRWAKEEAFWQAVRWFRLHLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEQ7</accession>
    <name>D5GEQ7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00001334001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430185">
        <property type="protein sequence ID" value="CAZ83000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838809.1">
        <property type="nucleotide sequence ID" value="XM_002838763.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEQ7"/>
    <dbReference type="FunCoup" id="D5GEQ7">
        <property type="interactions" value="45"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GEQ7"/>
    <dbReference type="GeneID" id="9184912"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001334001"/>
    <dbReference type="eggNOG" id="KOG3020">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031506_3_0_1"/>
    <dbReference type="InParanoid" id="D5GEQ7"/>
    <dbReference type="OMA" id="VPCFGWH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053044">
        <property type="entry name" value="Metallo-hydrolase/TatD-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001130">
        <property type="entry name" value="TatD-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47345">
        <property type="entry name" value="CUT9-INTERACTING PROTEIN SCN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47345:SF1">
        <property type="entry name" value="CUT9-INTERACTING PROTEIN SCN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01026">
        <property type="entry name" value="TatD_DNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="249"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="267"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="399" mass="44732" checksum="FD5C880A3D3F7B6F" modified="2010-06-15" version="1">MGSKDSFTESTWDLGIHDAHCHPTDTMSKAGTIPAMKAKSLTIMGTRKSDLPLVASLAKDYPEKVVPSFGYHPWFSYLIYDDTGSAGERVVDKQNHYTTVLTGAPTEDLVSALPAPRKLSSIITELRSHLETFPNALVGEIGMDRGLRIPFPENTSTEAHAVYIPGDTEDGKRLTNHRVSMDHQKKIFVAQLKLAGELNRAVSVHGVQCHGALFDIFRELWRGHEIEVESRKEMKRRRRRVKGGVDRHEYLPDIEESEDSDDEFSEEVGKQQEKKQEYTPKPYPPRICLHSFSAPAQTLQQYLTPPSPKKRYPSKVFFSFSSTINARPEKGHMEKIMETVKRVPGGRVLVESDLHTAGEEMDAALEGAVRVIAGIKGMGIDECVKVLGENWREFVFGDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>D5GF17</accession>
    <name>D5GF17_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00006699001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oligopeptide OPT transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430197">
        <property type="protein sequence ID" value="CAZ83110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838919.1">
        <property type="nucleotide sequence ID" value="XM_002838873.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GF17"/>
    <dbReference type="FunCoup" id="D5GF17">
        <property type="interactions" value="20"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GF17"/>
    <dbReference type="GeneID" id="9188648"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006699001"/>
    <dbReference type="eggNOG" id="ENOG502QQ2H">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010539_2_0_1"/>
    <dbReference type="InParanoid" id="D5GF17"/>
    <dbReference type="OMA" id="TPTAYVW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035673">
        <property type="term" value="F:oligopeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045035">
        <property type="entry name" value="YSL-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00728">
        <property type="entry name" value="OPT_sfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31645">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER YGL114W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31645:SF0">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER YGL114W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
        <property type="entry name" value="OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="86"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="180"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="238"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="275"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="372"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="490"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="544"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="570"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="613"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="137"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="159"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ83110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="644" mass="68516" checksum="45ABE6C7E157F666" modified="2010-06-15" version="1">MYFGLQTGWVSMMSMPSSLIGFAIFKSCAKHLEFPFTPVENVLVQTVAGAVGSMPLSAGFVGVVPALEKLLKPEEGGPLRIGTPQLMFWSIGVAFFGVFFAVPLRRQVIIREKLKFPSGTATALMISVLHGSGKVAARGGSGDEREGLVSGMDEEEEREGGREVQEDAGRKESWGQKNRLLVYSFALSGTCTFISYFFPILRDLPIFGSAAASTWLWTLNPSPAYVGQGIIMGTGTTLHMLLGAIIGWGVLSPFARYKEWAPGPVSDWTTGSRGWLVWVSLGIMLSDSVISLGGLILEPIIKSLWRRCKKVSRGREGYERLMDGGGGSEYEEGDEAWGLCRRRSGRSGVGEQGGESPGEDAPDEHLVPMKTVIIGLLASGLLCIIAVEVVFSMVPLYATAIAFLLALILSEMGVRALGQTDLNPVSGISKLTQLFFALITPPSHPSSIITNLVAGAISEAGAQQAGDIMQDLKTGHLLSASPRAQFHGQLIGSAFGAIISAGVYRLYTSVYPVPGKLFQIPTAYVWIDCSRLVFGQGLPEGARGFAVGFAVLFAVITALRGWATLGKGRWAGYLPGGIAVAVGMYNVPSFTLARAFGGLVQWWFGGRGQDAEAGLVVFASGLVLGEGVVSIVNLGMASFGVPHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5GF31</accession>
    <name>D5GF31_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">AP complex subunit sigma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00006722001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Adaptor protein complex 2 (AP-2) is a heterotetramer composed of two large adaptins (alpha-type subunit APL3 and beta-type subunit APL1), a medium chain (mu-type subunit APM4) and a small adaptin (sigma-type subunit APS2).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <location evidence="2">Coated pit</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the adaptor complexes small subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430197">
        <property type="protein sequence ID" value="CAZ83124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838933.1">
        <property type="nucleotide sequence ID" value="XM_002838887.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GF31"/>
    <dbReference type="FunCoup" id="D5GF31">
        <property type="interactions" value="446"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GF31"/>
    <dbReference type="GeneID" id="9187985"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006722001"/>
    <dbReference type="eggNOG" id="KOG0935">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061221_3_2_1"/>
    <dbReference type="InParanoid" id="D5GF31"/>
    <dbReference type="OMA" id="QSNFVEY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030122">
        <property type="term" value="C:AP-2 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035615">
        <property type="term" value="F:clathrin adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14833">
        <property type="entry name" value="AP2_sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000011">
        <property type="entry name" value="AP complex subunit sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016635">
        <property type="entry name" value="AP_complex_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027156">
        <property type="entry name" value="APS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11753">
        <property type="entry name" value="ADAPTOR COMPLEXES SMALL SUBUNIT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015588">
        <property type="entry name" value="AP_complex_sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0168">Coated pit</keyword>
    <keyword evidence="6" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AP complex mu/sigma subunit" evidence="13">
        <location>
            <begin position="3"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00062168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR015588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ83124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="144" mass="16950" checksum="4B318813DBADF04B" modified="2010-06-15" version="1">MVLSFILIQNRQGKTRLAKWYAPYDDEEKVKLKGEVHRLVAPRDQKNQSNFVEFRNNKIVYRRYAGLFFCVCVDANDNELAYLEAIHFFVEVLDAFFGNVCELDLVFNFYKVYAILDEVFLAGEIEETSKQVVLTRLEHLDKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFA3</accession>
    <name>D5GFA3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00006798001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430208">
        <property type="protein sequence ID" value="CAZ83196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839005.1">
        <property type="nucleotide sequence ID" value="XM_002838959.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFA3"/>
    <dbReference type="STRING" id="656061.D5GFA3"/>
    <dbReference type="GeneID" id="9188052"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006798001"/>
    <dbReference type="eggNOG" id="KOG4325">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074603_1_0_1"/>
    <dbReference type="InParanoid" id="D5GFA3"/>
    <dbReference type="OMA" id="IPGCADS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035145">
        <property type="term" value="C:exon-exon junction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903259">
        <property type="term" value="P:exon-exon junction complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039333">
        <property type="entry name" value="PYM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015362">
        <property type="entry name" value="WIBG_mago-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036348">
        <property type="entry name" value="WIBG_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22959:SF0">
        <property type="entry name" value="PARTNER OF Y14 AND MAGO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22959">
        <property type="entry name" value="PYM PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09282">
        <property type="entry name" value="Mago-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01273">
        <property type="entry name" value="Mago-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101931">
        <property type="entry name" value="Pym (Within the bgcn gene intron protein, WIBG), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WIBG Mago-binding" evidence="2">
        <location>
            <begin position="28"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="153"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ83196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="216" mass="23023" checksum="6D53F72521D13BC3" modified="2010-06-15" version="1">MSTSIPASDPAAETSTSAKSGITLTSDGQRVIPSSLRPDGSTRREIRVRPGYRPPEDVEIYKNRTAEAWKNRGSGGVPGASSPSDEDGKQPAGTSASSKNAKRKAARKRAKERVAGGQPEADGGAVGSSEKTNGSRNDDPASTTPTAGGSELEQTKTEPTPDEREKMAKAIRKKIRQANELKIRKEVGESLLPEQLEKVIKLQELTRQLGALGFDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFJ7</accession>
    <name>D5GFJ7_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00006940001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPL family. K-HECT subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="3"/>
    <dbReference type="EMBL" id="FN430209">
        <property type="protein sequence ID" value="CAZ83290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839099.1">
        <property type="nucleotide sequence ID" value="XM_002839053.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5GFJ7">
        <property type="interactions" value="1158"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GFJ7"/>
    <dbReference type="GeneID" id="9188238"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006940001"/>
    <dbReference type="eggNOG" id="KOG0168">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0170">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000366_1_1_1"/>
    <dbReference type="InParanoid" id="D5GFJ7"/>
    <dbReference type="OMA" id="AEPLSQF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045322">
        <property type="entry name" value="HECTD1/TRIP12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057948">
        <property type="entry name" value="TPR_TRIP12_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45670:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HECTD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45670">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25579">
        <property type="entry name" value="TPR_TRIP12_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="8">
        <location>
            <begin position="1453"/>
            <end position="1810"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="589"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="741"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1037"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1132"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1252"/>
            <end position="1272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1385"/>
            <end position="1404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="13"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="85"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="139"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="158"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="187"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="593"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="606"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="741"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="768"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1060"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1174"/>
            <end position="1185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1394"/>
            <end position="1404"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="1777"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ83290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1810" mass="197988" checksum="A02C8548A508E025" modified="2010-06-15" version="1">MPPRRVTRSSTRQLSDQAASLASNSNSQTPVATPPTATTSTAITATTTAAPSPSKPPRKRKNSATAAEASEPPTSQARSRKRSKAASFSSISAQQPEIPAAPQSSRRKGKGKRAELDMRDQGPAGEPSNSITTPDVATPRRRGSRSKKSAGDNPATPSSAFSSSSRRAAPKMNPEHNDPDVPMSEPNEVEEDNFDEDHDEDMADQGAGDDDDQDRDEDYEREDEDEDDVFEGSYLGGVGGSSGLSSTLRALSGMVSGIPSRLRDNLASLRQKDDPSTQLIALQELAEILLVSTEDNLSGHFSPDQFVKELVSLMEDQGPFGENPEMMLLACRCIANLMEALPAATANVVYGGAVPVLCRKLMEIQYIDLAEQALSTLEKISVEYPTSIVREGGLTACLTYLDFFATNVQRTAVTTAANCCRNIPDDCFPTVRDVMPILLNVLGSSDQKVVEQACQCVARIVESFRHYPDKLEQLMSKEMMNAILQLLLPGTTNLVGPYIHTQFLRVLSIIAKASPRLSVDMFKMNIVDTLYQILTGVSPPVAHDGAAMKNDSVMTMQALIHRPREQVSETLNVICELLPGLPHDDVFGAAFDSQDSSGGTTKAPSDEGEDDDDDPDDKRRKLLENCKGEMKRFATVLLPTLTDAYSSTVNLSVRQKVLNAQLKMISNLDADILKESLSSVQFASHLASILSQQDHPTLVLAALQAAELLLRRLPEIYQYHFYREGVISEISKLASKKDEQAALEKEAAERGDIDRESEDSRDSEDRMSSSPVSSRSSSSSRHVPASTSTGMADWITEKAKRFMEGHDKDENTETKLKAMTIMEELKSLATGLKSGDSTESLFERLATYFDNDNSLSSISSFELLNSHIVEALLAVLGSSSGMLTPFYLYVSDIRRAFLEVFMRSGAHPAASSEGDTPFSVLVHKLQDLLSRSEHFEVVTVHQNTFDGNRSSAASMLAKQLRLRLVADEDSEIPRPYRNIMVSIHAIATFKALDDYLRPRISLSERPRSGRRDPGGAGGGGLSNALAAFAAAAGLAAPNSRPGGLPGDAASSSTPAPPRPRQARRQSRNKRSNLEGASASSSRGKVPGAERENQPTQPSGQAPLECMDERPIPEDDDMDEMDTVLEDLDRDLDEEPEPEAGAVNLEVGTTGKVTARKDDGTRVATPSGAPPATRNPGSSQTSTPTNRGLPAGYPGAIPSPLDWHIEFSIGDHVISNDMTIYRAVQLGRNDTAEDQTFRNVWSTVHPIKFRRVTGPTPPEGSLSPAPDGSQISTGFPASLDKNKVSSSILQLLSILHALNANLDDVFTGDREAMKPKSPQPLSQFVNTKLTAKLNRQLEEPLIVASACLPSWSEDLARLYPFLFPFETRHLFLQSTSFGYSRSMTRWQNSQSTGDSRQDRHRDDNRPFLGRLQRQKVRISRLRILESAIKVMDLYGASPSVLEVEYFEEVGTGLGPTLEFYSSVSRAFARKKIKLWRENESSPESEFAFGQQGLFPAPMSDPMAESENGCKVLHLFTMLGKFVARSMLDSRIIDISFNPTFFRTGENVDEAVAPSLGAVKTVDKDLASSLKLLRKFALAKEEIDNNRVLTQGGKAKKAAQIMFDGVHVEELGLDFTLPGYPHIELIPGGSDVMVTIDNVKGYVDKVIDLTLGSGVRRQAEAFRSGFSQVFPYSALRAFTPDELVMLFGRNEEDWSLETLMDSIKADHGFNMDSKSVRNLLTAMSEFSAQERRDFLQFVTGSPKLPIGGFKSLTPLFTVVCKPSEPPYTSDDYLPSVMTCVNYLKLPDYSTFEILKKRLSIAIQEGQGAFHLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFM4</accession>
    <name>D5GFM4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Proteasome subunit alpha type</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00006985001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the peptidase T1A family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430209">
        <property type="protein sequence ID" value="CAZ83317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839126.1">
        <property type="nucleotide sequence ID" value="XM_002839080.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFM4"/>
    <dbReference type="FunCoup" id="D5GFM4">
        <property type="interactions" value="874"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GFM4"/>
    <dbReference type="GeneID" id="9188265"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006985001"/>
    <dbReference type="eggNOG" id="KOG0178">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035750_4_3_1"/>
    <dbReference type="InParanoid" id="D5GFM4"/>
    <dbReference type="OMA" id="YVLNDNM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034515">
        <property type="term" value="C:proteasome storage granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080129">
        <property type="term" value="P:proteasome core complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03752">
        <property type="entry name" value="proteasome_alpha_type_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000009">
        <property type="entry name" value="Proteasome subunit alpha type-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003075">
        <property type="entry name" value="PRK03996.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 5 6" id="KW-0647">Proteasome</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="7">
        <location>
            <begin position="5"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ83317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="249" mass="27574" checksum="EB53EAD1C468AAB1" modified="2010-06-15" version="1">MSRRYDSRTTIFSPEGRLYQVEYALEAISHAGTALGVLAKDGIVLAAERKVTSKLLEQDTSAEKLYILNDNMICAVAGMTADANILINYARQAAQNYLLTYNEDIPCEQLVRRLCDLKQGYTQHGGLRPFGVSFIYAGYDSQREFQLYQSNPSGNYGGWKATSVGANNASAQSLLKQDYKEDCDLKEACAIAVKVLSKTMDSTTLSNEKIEFATVGKTESGKVHHHLWSAEEIQSLLREHGLAKEEADD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFY2</accession>
    <name>D5GFY2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00001947001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430227">
        <property type="protein sequence ID" value="CAZ83425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839234.1">
        <property type="nucleotide sequence ID" value="XM_002839188.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFY2"/>
    <dbReference type="STRING" id="656061.D5GFY2"/>
    <dbReference type="GeneID" id="9181780"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001947001"/>
    <dbReference type="eggNOG" id="ENOG502QSZU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023536_1_0_1"/>
    <dbReference type="InParanoid" id="D5GFY2"/>
    <dbReference type="OMA" id="GCQDIER"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028942">
        <property type="entry name" value="WHIM1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42107:SF1">
        <property type="entry name" value="WHIM1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42107">
        <property type="entry name" value="YALI0D24453P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15612">
        <property type="entry name" value="WHIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WHIM1" evidence="4">
        <location>
            <begin position="167"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="349"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="527"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="36"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="356"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="452"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF15612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ83425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="586" mass="63801" checksum="CC20DCFA998B22DA" modified="2010-06-15" version="1">MSSGLDSPLSSLLSSPPASDDEISPPSPVAAAVTKGKGKAVSRTKPTPKKGVKGKQQQKLPFVRKASTPPRKVKKKEPPHVPTLADSPELAFIVMFRSRFTDAFKGVPNLGCQDIERGIVDSTPSEQVEQLLCKLLSLVLNRKKPVERGHNQRALEEAANAHRNQWPASWEGKSPFSGNKSFGDLDTAQRLEILEALIHWSLSSSEVIRGIINDSYKGSRRDDDLNVPLSVQPWGRDADKRRYWLIEGRDDTPFRLYRESNPALKTHTWISVAGTIEEIQTVAKELEEEDGSKHALALKEKIIGAIPRFEEGETRRKKREYRASRKAFFNQPSGVSLYEGRTRGKRIRYNFSTDEEGTKETSEQDDSRSRRSARSNKGSPAPQPLPQPEVPIFTASGRQIRKPVTGDYGEVKINGSNGTAVGSRGTPFGSENGDYNNRGLPLVGVVVYKGDDGDDNEGDDEREESEDEAGWGSGPGGVEDEDAPRKSLRIVFKIPKAPGPADGASPAAGNNGVVSPSKAHQIVRVAGGEPTPTHTPVPHGNGLKANGVHKRDAEDVTMSDIEPIKEVNPAVNGAKPPVDQKKGLSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>D5GG89</accession>
    <name>D5GG89_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00007263001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430253">
        <property type="protein sequence ID" value="CAZ83532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839341.1">
        <property type="nucleotide sequence ID" value="XM_002839295.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GG89"/>
    <dbReference type="GeneID" id="9183157"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007263001"/>
    <dbReference type="eggNOG" id="ENOG502QWFN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011148_0_0_1"/>
    <dbReference type="InParanoid" id="D5GG89"/>
    <dbReference type="OMA" id="NKACFGQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010856">
        <property type="entry name" value="Gig2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30613:SF1">
        <property type="entry name" value="DUF1479 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_5G09280)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30613">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN YBIU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07350">
        <property type="entry name" value="Gig2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ83532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="405" mass="46059" checksum="DFF2F4B5FED4E703" modified="2010-06-15" version="1">MRRTCAIATRLIAPTTATRLIATKTQSKVGGDISSVFRSLSGIPPPPLSNRYAKLKSRFTSGRRRILAEPWERLLLDLEKAIAVVKRYGIDIIPQVQYKDLACLSKEEIAEIKKRGVVVVRNVIPDEEALAMKKSLKDHIKRYQWTKAFPADSPAVYELYWSLAQRAARAHSPLVASQRFVNSLWHSSLPSSVVDTSVSLPYADRFRIRKPGDAGFALEAHIDGGSLERWEDPEYSRCYEKIWTGQWEEYDPYNAEHRVHAKMDMYDGAGSCGVWRSWQGWLSLSTTYPGEGTLLVNPLLKHTTAYTILRPFFTLSNPPTLSTTPRFPNSVQGACQEYSNTTHPHLCLADSMTHVPRVSPGDYDFWHCDTIHAVDRQVSRYILHHIPKRPTYILNHDQANEMRIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGB1</accession>
    <name>D5GGB1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00007304001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430253">
        <property type="protein sequence ID" value="CAZ83554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839363.1">
        <property type="nucleotide sequence ID" value="XM_002839317.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGB1"/>
    <dbReference type="GeneID" id="9183152"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007304001"/>
    <dbReference type="eggNOG" id="ENOG502RDYZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009306_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGB1"/>
    <dbReference type="OMA" id="PPDMNYR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd13316">
        <property type="entry name" value="PH_Boi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09535">
        <property type="entry name" value="SAM_BOI-like_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAM" evidence="3">
        <location>
            <begin position="198"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="800"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="268"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="440"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="699"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="145"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="276"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="329"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="355"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="441"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="744"/>
            <end position="753"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ83554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="944" mass="104648" checksum="14A9A442A3D9EF89" modified="2010-06-15" version="1">MEAPEDGLEDLDHSDAESIGSLSSRAQRMNEMLSDSDSSIEGRASTDGERTFTSLSSYDEPRTPKSPAPEYSAHGFPMIESSDEKNPELFRRYSVEPSPTYQRDSISTIGPKGPHLFRSYSASHVTSIAHITIPHSGIVMPPTPSASASPSSPPAAVSPSHFPAPPQSPHYAESPQVRPMSELSAVVSDYEIDEVKSWSPKQVCAWMLALGFERELVEKFERNDISGAILVDLKWEDLKELDIHSFGKRIELWSEIHHLRARTATSPPMAQEPFVRTSSRVSRNSSTRSKTSRSPKFARGSQMVVREVGHMRSSSGAKALPDLPTLLPRSDRRKEKVISEREESSDEEHASRPIRQMRKRGRKTNVRVPRKQQFSVESIDSETASSEPAFEQYGHSLRYMPSIDDIEGHVVIRARRAKSPATRRRRKSVVEVLPNEISRKPSTKHKCKKHDGKCEHKHHKKPHRCSKGDKCRKHGGNGSGSKSSGSKRVPSLEHGTILIATTPSIADANPPRISALDSGSERPTRPGSLATISVLASSDVLGPTHGSEVKLRENTLKDVARMDPLENVKQFLTLQHLQHLEERRESPPPVPTKSAEHLARARGTYMPPTPSATPSPPLFPRSDSLLPPTSSHSAKSVLVPLAPAIQLPPTPTPSPPMRSQTPSLGLRIQGMERPAPPSINNATSPISRMRSPSNLLRTTTPFSEADVPYPSATPVPRNMSNSAPPDMRFRRFPTPDFSQQHSNPPLPTTPLPLPSRRGTSASASHFRPQPLHMTVVDENAEWEPIDEDEEVKMAPSPNPSKMHSGWMKKRRTNWFRHEWADYHFVLKGTRLGYAKNTEKEDGFIEMDNYSVSCSNSASHKLSAAFKGARLFGRKTSVVGEGTGAYFFQLVPAAPEGGRNAVGKVHYFAVGSREERIDWMRELMLAKAIKQKKEGFEVEVNGERV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGS8</accession>
    <name>D5GGS8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00007497001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430275">
        <property type="protein sequence ID" value="CAZ83700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839509.1">
        <property type="nucleotide sequence ID" value="XM_002839463.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGS8"/>
    <dbReference type="FunCoup" id="D5GGS8">
        <property type="interactions" value="13"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GGS8"/>
    <dbReference type="GeneID" id="9181670"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007497001"/>
    <dbReference type="eggNOG" id="KOG0929">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013090_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGS8"/>
    <dbReference type="OMA" id="TRMLCSK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1000.11">
        <property type="entry name" value="Arf Nucleotide-binding Site Opener,domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041681">
        <property type="entry name" value="PH_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023394">
        <property type="entry name" value="Sec7_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000904">
        <property type="entry name" value="Sec7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035999">
        <property type="entry name" value="Sec7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663">
        <property type="entry name" value="GUANYL-NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663:SF373">
        <property type="entry name" value="PH AND SEC7 DOMAIN-CONTAINING PROTEIN C11E3.11C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15410">
        <property type="entry name" value="PH_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01369">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00222">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48425">
        <property type="entry name" value="Sec7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50190">
        <property type="entry name" value="SEC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SEC7" evidence="2">
        <location>
            <begin position="242"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="399"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="557"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ83700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="963" mass="106486" checksum="0C937EADADBC4439" modified="2010-06-15" version="1">MNPYLRSPVKSPGFDLVNENNESAYLQRGATIRTVASSDPISPKRPTFFGESMRHRDSGSHFNDDYNSPGNVYRKETRYARGNGNVDHSPSTRDDWDRPQTSPYSPNKQSFLQDDEEKEVRWPPLATKSILSTRRSVGNVALERERQASIASSFDKPRQGSMVAKEQPIATMQEIEAHHAKLEKQQKEARAGKALEVDTENLENGGSREAETSWLSTLTPAAIGTPTVMLQRDGPGEKAEPLRGDDPDGEEPSEEERETARRVFKGDEEVITKARATSWLGEAGATGARVRKAYMELFDWSGVNILSTLRIFCGRLVFKGETQQVDRIMDAIAKRWCECNPRHGFKLIDVVFTILYSLLLLNTDLHMADIPQSQKMTKGQFIKNTMSSIRRIVTEAPVESKPVHHNTFPPRSQTPFRPNDDDDNPGMSATPSATFPLAVRERKMSESRTSLDFKMGYDMDGGLALVKAPLGGGGGGIRAWETQIETVLKDFYSSVKASALPLHGAAQERVVETPHHSSLARTFQSLAHKARCTTRNNRDSKLGAKWTAKNRSRPRLYNGSYAGSSRTSLEDRSMWSPSASSTWSKYSLDKTQTSMSVDSLGSAFTHADYAQSIGFANALSHAIIREEAASGTSVDDREATLLDCDELELAGAPWAKEGMLKHKHHLESMDKKAKNRGWTECFAVIERGWMRLFQFNQSGKNKDAVVGVVGGGNWTENADAIGSFLLRQTIALALPPPGYSKARPNVWALSLPSGAVHFFEVGTAEIVKEFVSTANYWAARLSKEPLIGGVSNVEYGWSDNVLGEIGPGLTSSVSNTNDSRPSLQISIRSSLDHGTVVRPRLPGDRVNITDWAPPSQSMVASSLGEADQLRALETYVTNIEVELARHNDLRPTMVLTFSPRHPNSSKAMNNWEKKSSYLLREIIKFRTYIECLSSAAQLREKLQAGENGAVSGNNNESRSPVST</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGZ7</accession>
    <name>D5GGZ7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007633001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430297">
        <property type="protein sequence ID" value="CAZ83790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839599.1">
        <property type="nucleotide sequence ID" value="XM_002839553.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGZ7"/>
    <dbReference type="STRING" id="656061.D5GGZ7"/>
    <dbReference type="GeneID" id="9185044"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007633001"/>
    <dbReference type="HOGENOM" id="CLU_704360_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGZ7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="360"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="383"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="392" mass="43167" checksum="CF369285D1770BD6" modified="2010-06-15" version="1">MLETKIFKPYDKKLDTEEWPQLQLVDAVVYRHVADGPDELCDLLDTHFMGPLRITGTITKIPAKFNKMVKAAKTDLPVIGEEQASSRTAAALAKGVHGATVTVTDALTYALESYKNPKVNSTIWALGCAAWYSITPAPEYTKYFDRSLEKAHVWDIVLEFGNSGNFKQVTVEQLFARYFQKHPGCKGIDGAENVFANYRKFLNFCLWKWLTAKFTGIVKEVEGSIQAAKTARKSSTDKRAFSPGTQHGVDTLESLALEQKLKPHQLTFEWLISSIEKKSGANAEVVRSLLQGQANALSTLMTLSRQVNWVQSSLYSDLLLMSGEVPSGGNCDGSGGGEAATTANLNPTKSRLVDAFDGVNDIEQDNDPLSSPELPHHQQPKSAKGKSVLRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5GH85</accession>
    <name>D5GH85_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Exocyst complex protein EXO70</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00007792001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in the secretory pathway as part of the exocyst complex which tethers secretory vesicles to the sites of exocytosis. Also plays a role in the assembly of the exocyst.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Bud</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Bud neck</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the EXO70 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430308">
        <property type="protein sequence ID" value="CAZ83910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839719.1">
        <property type="nucleotide sequence ID" value="XM_002839673.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GH85"/>
    <dbReference type="FunCoup" id="D5GH85">
        <property type="interactions" value="115"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GH85"/>
    <dbReference type="GeneID" id="9182615"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007792001"/>
    <dbReference type="eggNOG" id="KOG2344">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010236_4_2_1"/>
    <dbReference type="InParanoid" id="D5GH85"/>
    <dbReference type="OMA" id="GIIRAGP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.170">
        <property type="entry name" value="Exocyst complex component Exo70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004140">
        <property type="entry name" value="Exo70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046364">
        <property type="entry name" value="Exo70_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12542:SF41">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12542">
        <property type="entry name" value="EXOCYST COMPLEX PROTEIN EXO70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03081">
        <property type="entry name" value="Exo70_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20669">
        <property type="entry name" value="Exo70_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex subunit Exo70 C-terminal" evidence="5">
        <location>
            <begin position="239"/>
            <end position="603"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU365026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ83910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="605" mass="67587" checksum="E0593B7452C7E406" modified="2010-06-15" version="1">MNGLRMDELSAEVEVLESKLSKTADLSTKIALSLKKLSASAQNVEQAVKPIYNKTQSLTVLSGNIDEAISAIDRVRQPTDAVSKEEGTIRQGPSKTGLTEYLASLRRINEALSSLKKSNLRSSQKAVTQMTGLLKAGSLQLEDLFRQALAEGSKSVEPLHFITKELPFPTFQPQKINMLAVLNDFLSSTLAASAGIQSNAPQVYAEVRGPYITNSLASLALATVSTTRRTSAAPYDKGSNGISVYTNALEAIFEAEYENICQLFPTPEWSRVYIATTAMPMGVFKKTVADLNVFVKQNMATDCFLAFDVIENVQPASVRLKTKTGEQKEFAEALKPLRTTAQSSFSYFLEDIKKTGQGLIALPLDNTVAEMTVNVMSRLRRMADYPNAISSLLVSLGEGNWNRPYTAPAVIPPSFDVGADGTLLLSNFCLDAIDQLIHELEQKARVMIKKNSTVAVFMVNNVHFIESNIRTSDLRKIMSNQAQAKVEKWRKDAVKMYMEQWKECAAFLMDVTYTKQQSGGRLNLNSKEKEGVKEKFKNFNTVFEELIQKHKSYTFPDKEVRTMLSKEIGFIGPLYGRFYDKYKDLMRDKYVKYDRHQLDTMLAQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHI7</accession>
    <name>D5GHI7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007935001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430319">
        <property type="protein sequence ID" value="CAZ83980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839789.1">
        <property type="nucleotide sequence ID" value="XM_002839743.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHI7"/>
    <dbReference type="STRING" id="656061.D5GHI7"/>
    <dbReference type="GeneID" id="9183634"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007935001"/>
    <dbReference type="HOGENOM" id="CLU_166294_1_0_1"/>
    <dbReference type="InParanoid" id="D5GHI7"/>
    <dbReference type="OMA" id="ACNTAYG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37475">
        <property type="entry name" value="ZYGOTE-SPECIFIC CLASS V COPY B GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37475:SF1">
        <property type="entry name" value="ZYGOTE-SPECIFIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072785" evidence="1">
        <location>
            <begin position="19"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="83" mass="7925" checksum="5AF1A63E3FC42683" modified="2010-06-15" version="1">MKLQNLLLPMSFATVALAGPISYGICQSGCAAVVCVCYSAAGAVFGTVPAAAAAAMPALAACNGAFGTCSATCAGVTLLAPIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHU2</accession>
    <name>D5GHU2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00008113001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430321">
        <property type="protein sequence ID" value="CAZ84085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839894.1">
        <property type="nucleotide sequence ID" value="XM_002839848.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHU2"/>
    <dbReference type="GeneID" id="9184178"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008113001"/>
    <dbReference type="HOGENOM" id="CLU_2814291_0_0_1"/>
    <dbReference type="InParanoid" id="D5GHU2"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ84085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="67" mass="8098" checksum="235B647EF47BA034" modified="2010-06-15" version="1">MGGKEGENHWRTHQSRVYLRTWYHTLPHFLTLSCTIILCNCHIYPKYLSTLYTCTVQYFYTHINKQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5GI33</accession>
    <name>D5GI33_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">thioredoxin-dependent peroxiredoxin</fullName>
            <ecNumber evidence="2">1.11.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00008248001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a hydroperoxide + [thioredoxin]-dithiol = an alcohol + [thioredoxin]-disulfide + H2O</text>
            <dbReference type="Rhea" id="RHEA:62620"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.11.1.24"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peroxiredoxin family. AhpC/Prx1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.24" evidence="2"/>
    <dbReference type="EMBL" id="FN430322">
        <property type="protein sequence ID" value="CAZ84176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839985.1">
        <property type="nucleotide sequence ID" value="XM_002839939.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GI33"/>
    <dbReference type="FunCoup" id="D5GI33">
        <property type="interactions" value="1009"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GI33"/>
    <dbReference type="PeroxiBase" id="11115">
        <property type="entry name" value="Tmel2CysPrx"/>
    </dbReference>
    <dbReference type="GeneID" id="9181894"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008248001"/>
    <dbReference type="eggNOG" id="KOG0852">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042529_21_1_1"/>
    <dbReference type="InParanoid" id="D5GI33"/>
    <dbReference type="OMA" id="FWYPKDF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061692">
        <property type="term" value="P:cellular detoxification of hydrogen peroxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900745">
        <property type="term" value="P:positive regulation of p38MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03015">
        <property type="entry name" value="PRX_Typ2cys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000003">
        <property type="entry name" value="Peroxiredoxin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050217">
        <property type="entry name" value="Peroxiredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024706">
        <property type="entry name" value="Peroxiredoxin_AhpC-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019479">
        <property type="entry name" value="Peroxiredoxin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10681:SF171">
        <property type="entry name" value="PEROXIREDOXIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10681">
        <property type="entry name" value="THIOREDOXIN PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10417">
        <property type="entry name" value="1-cysPrx_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000239">
        <property type="entry name" value="AHPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 9" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="7 9" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="11">
        <location>
            <begin position="3"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate; for peroxidase activity" evidence="10">
        <location>
            <position position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000239-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ84176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="196" mass="21631" checksum="380B5DD86453585E" modified="2010-06-15" version="1">MVARVQHPAPPFKGTAVVEGGFEEISLKDYEGKWLILGFIPMAWTFVCPTEIIAFSDAAKSFEERGASIVFASVDSEYSLLAWSSTERKDGGLGNINIPLFSDKNHKLAGDYGVLIEEEGVALRGLFIIDPKGTIRQITINDLPVGRSVDETLRLVDAFKFTEKYGEVCPANWNQGGETIKANPKESKEYFNKVHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5GI61</accession>
    <name>D5GI61_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00008301001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430323">
        <property type="protein sequence ID" value="CAZ84204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GI61"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008301001"/>
    <dbReference type="HOGENOM" id="CLU_2706595_0_0_1"/>
    <dbReference type="InParanoid" id="D5GI61"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ84204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="73" mass="8266" checksum="9E2F31D59672CE56" modified="2010-06-15" version="1">MYFIGGAVVPCHFHTLDENPLSLYFLPHCFRNGGAMGAKLWVQDRLLMADILVELRGIAVVLKWNAARVWGHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>D5GI67</accession>
    <name>D5GI67_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00008308001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430323">
        <property type="protein sequence ID" value="CAZ84210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840019.1">
        <property type="nucleotide sequence ID" value="XM_002839973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GI67"/>
    <dbReference type="FunCoup" id="D5GI67">
        <property type="interactions" value="71"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GI67"/>
    <dbReference type="GeneID" id="9181928"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008308001"/>
    <dbReference type="eggNOG" id="KOG4561">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034597_0_1_1"/>
    <dbReference type="InParanoid" id="D5GI67"/>
    <dbReference type="OMA" id="AQWYNGM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006634">
        <property type="entry name" value="TLC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050846">
        <property type="entry name" value="TLCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13439">
        <property type="entry name" value="CT120 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13439:SF0">
        <property type="entry name" value="TOPOISOMERASE I DAMAGE AFFECTED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03798">
        <property type="entry name" value="TRAM_LAG1_CLN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00724">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50922">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="275"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="TLC" evidence="8">
        <location>
            <begin position="68"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="311"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ84210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="364" mass="41237" checksum="599808E72908DA37" modified="2010-06-15" version="1">MASSILFAPCPPLTQLVKPYAAKIGLHSLPFHIHQVIAAVVVYSLIYVVISPILSSIFSETYRNFDKRTRVNWNVHVVSFVQSIFICALAIWAACNDPTRDAWSTDSVAILKRTFGHSKTQGAVQAYAEGYFIWDLFISAWHLDIFGLGFLAHAASAVMVFSLGFRPFVNYWASVFVLFEISSPFLNIHWFCDKTGRTGSLIQLVNGFFLLTSFFCCRLIWGTWNAFLVFGDLHTLYNNPPAPVLPPSDDSLIHLDHLETDKIVAYLFAGKNLPLWLIMVYLGSNAILNLLNYYWFYRMIQTVSARFTKDKPVKKEKKEKKEKKENTEVDADGKVWVEGTDVNVGTTAAVTKEKLKKRTVTRQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIA8</accession>
    <name>D5GIA8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00008389001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430325">
        <property type="protein sequence ID" value="CAZ84251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840060.1">
        <property type="nucleotide sequence ID" value="XM_002840014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIA8"/>
    <dbReference type="STRING" id="656061.D5GIA8"/>
    <dbReference type="GeneID" id="9184610"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008389001"/>
    <dbReference type="HOGENOM" id="CLU_674720_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIA8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.180.10">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036378">
        <property type="entry name" value="FAS1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000782">
        <property type="entry name" value="FAS1_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02469">
        <property type="entry name" value="Fasciclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82153">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50213">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072173" evidence="3">
        <location>
            <begin position="22"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="389"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="4">
        <location>
            <begin position="251"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="141"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ84251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="408" mass="42587" checksum="4B609B45A80BFE78" modified="2010-06-15" version="1">MILPIPLLAVVLLSLAPYVSCQQFTSNQTLMEVLEGMPDCQEYFKLLQRYPAAQSPPPGPLPFTVFCPSNEAVIRKLAEPSGSIFKRDDPKATSSMAANTATGVSVTKPPRPPVTPAPRARAKRQVTSSLTGRPTVIVSLPTAAGATTTGTTSATPGSSPAGSPAASTAVSTNFRTSGPDPFALDPNQLTLKTTLDNPEVVNLGPGAFLPMVSFTQAPSVLGLACGLGEVVNVNPRSIPFDRGEVRVSAGFVTLPETISQSLTFLAQRTFLEALRRFRLLSFYETTPLITAFVPIDSSLSGELSECACKHHIVRGPPLYTPDIELGRAYTTDAGGTITIEIKDGVYTLTGGAVIEKANVITPNGVVHLIKGNISGICEPMAFTGGSSRVGAWAGGLVGLVGVVVGLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIB3</accession>
    <name>D5GIB3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00008396001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430325">
        <property type="protein sequence ID" value="CAZ84256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840065.1">
        <property type="nucleotide sequence ID" value="XM_002840019.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIB3"/>
    <dbReference type="GeneID" id="9184615"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008396001"/>
    <dbReference type="HOGENOM" id="CLU_2759649_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIB3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ84256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="70" mass="8195" checksum="F1F073D17E6C4EFB" modified="2010-06-15" version="1">MVRWMKFAGWWLRGSVHEYISEDCSAILRPYSCLLKLTDASPSRKVYKTNESRAGIKYTHRLVSKLCTVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>D5GII8</accession>
    <name>D5GII8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00008519001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430327">
        <property type="protein sequence ID" value="CAZ84331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840140.1">
        <property type="nucleotide sequence ID" value="XM_002840094.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GII8"/>
    <dbReference type="GeneID" id="9183265"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008519001"/>
    <dbReference type="HOGENOM" id="CLU_1836581_0_0_1"/>
    <dbReference type="InParanoid" id="D5GII8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ84331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="140" mass="15586" checksum="848CAC99228DBA5B" modified="2010-06-15" version="1">MAQNIFFTLASVTAIILSLICVRSILLRRAKIARQERAAEILRQAEVGIQAGQWARVTHVNSILRPAPVYLHNTRSPPPAHVHPNTNEEVAIEPLPVYEAPPPKYDSVVKEAQPPVEMRELERGEPSGRAGPPEYNQERG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIL5</accession>
    <name>D5GIL5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00008551001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the import of GDP-mannose from the cytoplasm into the Golgi lumen.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homooligomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TPT transporter family. SLC35D subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430327">
        <property type="protein sequence ID" value="CAZ84358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840167.1">
        <property type="nucleotide sequence ID" value="XM_002840121.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIL5"/>
    <dbReference type="GeneID" id="9183292"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008551001"/>
    <dbReference type="eggNOG" id="KOG1441">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027744_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIL5"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021838">
        <property type="entry name" value="DUF3431"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004853">
        <property type="entry name" value="Sugar_P_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11913">
        <property type="entry name" value="DUF3431"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03151">
        <property type="entry name" value="TPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="23"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="89"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="112"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="173"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="206"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="237"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="267"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="294"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="339"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar phosphate transporter" evidence="9">
        <location>
            <begin position="23"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ84358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="654" mass="72619" checksum="5AD74AF45320B651" modified="2010-06-15" version="1">MTPAIGTPPPAALGKPRDRIHPAFYVIVWISLSSAVILFNKWILDPGTKNFAIFLTTWHLLFSSIVTQFLARTSTLLDGRKAVKMTGKVYLRAICPIGLFFSLSLVCSNKAYLYLSVSFIQMLKATTPVAVLIASWSLGVESLNLSVLRNVTFIVIGIMIASYGEILFDPSGFIFQVFGIGFEAVRLVMVQRLLSSAELKMDPLVSLYYFAPICAAMNFVLFLIFESSSLGVSELLMIGWLTFLLNALVAFGLNVSVVFLIGKTSSLVLTLCGVLKDILLVCASMIIWGNPVTILQFFGYSIALSGLLYYKLGADKINEQYAHLRGLRLGTGSLSNRRLGIGAASLFTVIVLGTMYHGRYPTRDATFVGAHTSYSSSRFITGNRVSRSRFDIVISMSQENTQTIRDRVEEILSLPQLSTLETKVVVYSMDKTADLVSLIKETGADAAESIPNISRETGAYLHHILKHWDNLADHTLFLHPDIEDFDHVKARIEDFFLPSTGMLSLGIGHAECSCNECRDPWASADTWARVPQIFSAVYGELCPGTSILLSRGSGFIVSSRRVKGTDKHIYQHLKEVLESDEKHWIHQDTRQPGMEDAPQNPYFGHALERSWMIVFKCSEMRLVKSCPKLDTRRKPDDWDDVCQCLDTGLGGHSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJ30</accession>
    <name>D5GJ30_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glutamine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Glutaminyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">GSTUM_00008811001</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>tRNA(Gln) + L-glutamine + ATP = L-glutaminyl-tRNA(Gln) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:20121"/>
            <dbReference type="Rhea" id="RHEA-COMP:9662"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.18"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.18" evidence="2"/>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84523.1"/>
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        <property type="nucleotide sequence ID" value="XM_002840286.1"/>
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        <property type="interactions" value="1243"/>
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    <dbReference type="STRING" id="656061.D5GJ30"/>
    <dbReference type="GeneID" id="9181864"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008811001"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001882_2_3_1"/>
    <dbReference type="InParanoid" id="D5GJ30"/>
    <dbReference type="OMA" id="TWCIYPM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004819">
        <property type="term" value="F:glutamine-tRNA ligase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990825">
        <property type="term" value="F:sequence-specific mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006425">
        <property type="term" value="P:glutaminyl-tRNA aminoacylation"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.240.10:FF:000007">
        <property type="entry name" value="Glutamine--tRNA ligase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Glutamine--tRNA ligase cytoplasmic"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Glutaminyl-tRNA synthetase"/>
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        <property type="entry name" value="Glutaminyl-tRNA synthetase"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glutaminyl-tRNA synthetase, non-specific RNA binding region part 1, domain 1"/>
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        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.240.10">
        <property type="entry name" value="Ribosomal Protein L25, Chain P"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
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    <dbReference type="InterPro" id="IPR004514">
        <property type="entry name" value="Gln-tRNA-synth"/>
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        <property type="entry name" value="Gln-tRNA-synth_Ib_RNA-bd_2"/>
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        <property type="entry name" value="Gln-tRNA-synth_Ib_RNA-bd_N"/>
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        <property type="entry name" value="Gln-tRNA-synth_Ib_RNA-bd_N_1"/>
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        <property type="entry name" value="Gln-tRNA-synth_Ib_RNA-bd_N_2"/>
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        <property type="entry name" value="Gln/Glu-tRNA_Ligase"/>
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    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020059">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_codon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020056">
        <property type="entry name" value="Rbsml_bL25/Gln-tRNA_synth_N"/>
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        <property type="entry name" value="Ribosomal_bL25/Gln-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049437">
        <property type="entry name" value="tRNA-synt_1c_C2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00440">
        <property type="entry name" value="glnS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43097:SF4">
        <property type="entry name" value="GLUTAMINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43097">
        <property type="entry name" value="GLUTAMINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03950">
        <property type="entry name" value="tRNA-synt_1c_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20974">
        <property type="entry name" value="tRNA-synt_1c_C2"/>
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    </dbReference>
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        <property type="entry name" value="tRNA_synt_1c_R1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="tRNA_synt_1c_R2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
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    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribosomal protein L25-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutaminyl-tRNA synthetase class Ib non-specific RNA-binding" evidence="15">
        <location>
            <begin position="10"/>
            <end position="166"/>
        </location>
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        <location>
            <begin position="170"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamyl/glutaminyl-tRNA synthetase class Ib catalytic" evidence="12">
        <location>
            <begin position="252"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamyl/glutaminyl-tRNA synthetase class Ib anti-codon binding" evidence="13">
        <location>
            <begin position="559"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA synthetases class I (E and Q) anti-codon binding" evidence="16">
        <location>
            <begin position="671"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="182"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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            <dbReference type="ARBA" id="ARBA00022917"/>
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            <dbReference type="ARBA" id="ARBA00048270"/>
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            <dbReference type="RuleBase" id="RU363037"/>
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    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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            <dbReference type="Pfam" id="PF00749"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF03950"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF04557"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF04558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF20974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAZ84523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="791" mass="89586" checksum="978F57DC698F3756" modified="2010-06-15" version="1">MSEPTPEEVLTEKFPKLGLGEKPLKEALKNKKLTTAWAQVLSNIDLPVDAKVGALLSAVVTAMKDVGGDDGKRAFVVDAVLSRKLKTNPQIEAAVKYIKGKKGDVHVAEFDKECGVGVEFTSEKIEQEVRAYIEEHKSTILEQRYKAFTPTLVTLKTSTDLKWAPPADLKKEVDAQLAALLGPKDERDAPPKKQPKAPAAAGAKQVKVENEDISKNRMFEEGFLAGLHRPGGNEQIVPTNMEAHLKATGGRPNGYLHIGHSKAITINFGFARYHGGECYLRYDDTNPEAEEERYFTAIREIVEWLGFQPYRITYASDHFQRLYELAEDLIKRGKGYICHCTAEEVNLQRGGKDNGGKRFECKHRNRPIEESLQEFRNMRGGKYKPKEAMLRMKQNILGSGNPQMWDLTAYRVLDGAPHHRTGSEWNIYPTYDFTHCLCDSFENISHSLCTTEFINSRESYDWLCDALGIYRPQQREYGRLNLTGTIMSKRKIAKLVSGGYVRGWDDPRLYTLVAIRRRGIPPGAILAFVNELGVTTAHSYVQIKRFEQTVRRYLEETVPRLMMILDPIPIIIENLPEDHLEEFSVPFMPNDSKLGEHLVPFTRKVYIDRSDFREEDSADYFRLAPGKSVGLLKVPFTIRATSYTKDKAGNVTEVRAEYENTGKFKKPKTYIQWVCDAPSKGSPVRVEARLTNQLFKSENPEDNPDSYLADINPDSEHVYKDAIIETGIHEVHRRAPWPELEGEKNSNRAGPESCRFQALRVGYFALDSDSTDDRFVLNRIVTLKEDAGKSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJ55</accession>
    <name>D5GJ55_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">amidase</fullName>
            <ecNumber evidence="3">3.5.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00008844001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a monocarboxylic acid amide + H2O = a monocarboxylate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:12020"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:35757"/>
            <dbReference type="ChEBI" id="CHEBI:83628"/>
            <dbReference type="EC" id="3.5.1.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amidase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.4" evidence="3"/>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840357.1">
        <property type="nucleotide sequence ID" value="XM_002840311.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJ55"/>
    <dbReference type="FunCoup" id="D5GJ55">
        <property type="interactions" value="47"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJ55"/>
    <dbReference type="GeneID" id="9181889"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008844001"/>
    <dbReference type="eggNOG" id="KOG1212">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009600_9_2_1"/>
    <dbReference type="InParanoid" id="D5GJ55"/>
    <dbReference type="OMA" id="HEVFFDQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004040">
        <property type="term" value="F:amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46072:SF2">
        <property type="entry name" value="AMIDASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46072">
        <property type="entry name" value="AMIDASE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001221">
        <property type="entry name" value="Amidase_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="6">
        <location>
            <begin position="72"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="128"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="205"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="5">
        <location>
            <position position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001221-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ84548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="550" mass="60029" checksum="44A1FAF63818BD8B" modified="2010-06-15" version="1">MTKNWKSIAEAKNAETKAKIPREWLIPLPPAEIRDVTGYASQFLTPAEIAITESTAHELVGKLAYGKYSSLEVTKAFCHRATIAHQLTNCLMEIFFDKAFKRAQELDDYFHEYKKISGPLHGLPVSLKDSVRIEGEQTTIGYVGWIGKNDTSESEALVTKILRNAGAVFYVKTSVPSGLMSGETVNNIVGYTWNPKNRLLSSGGSSGGEGALIAIRGSPMGVGSDLAGSIRIPAGATGIFGLKPSCGRLPYKNIAQSPNGQETVGGVVGPMTNSIADARLFVQTILEAEPWKHDPGVIELKWRPLEEDETREHAKRGLSFAVMKHNGEITPHPPILRALDIAAAKLRSQGHEVIEWTPPSHKIANSMLLEALSADGGAHTRRNHALSGEQVLPQIAASFSTTLGSLHEKSMTELWDLHVRKLTFQDAYQTYWNSTASLTKTGRPVDALILPWAPSASYPPEKFCYLGYSGFVNLLDYTASVVPVGVKVDKSIDRKAVGYEPVDALDKFVYESYDPEVYHGAPIGIQIVGRRLQEEKVLVISEVVEAALKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJ67</accession>
    <name>D5GJ67_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00008863001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840369.1">
        <property type="nucleotide sequence ID" value="XM_002840323.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJ67"/>
    <dbReference type="STRING" id="656061.D5GJ67"/>
    <dbReference type="GeneID" id="9185928"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008863001"/>
    <dbReference type="eggNOG" id="KOG2877">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035066_5_0_1"/>
    <dbReference type="InParanoid" id="D5GJ67"/>
    <dbReference type="OMA" id="GMWMYST"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016780">
        <property type="term" value="F:phosphotransferase activity, for other substituted phosphate groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1760:FF:000029">
        <property type="entry name" value="CDP-alcohol phosphatidyltransferase protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048254">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014472">
        <property type="entry name" value="CHOPT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10414:SF77">
        <property type="entry name" value="CDP-ALCOHOL PHOSPHATIDYLTRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10414">
        <property type="entry name" value="ETHANOLAMINEPHOSPHOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015665">
        <property type="entry name" value="CHOPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="64"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="99"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="238"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="304"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="332"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="371"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ84560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="427" mass="48455" checksum="432CC4512466EC17" modified="2010-06-15" version="1">MASNRVDNRPKGSRGRRRSSEFLSEDQLVHLKTYRYQSVDKSLISRYILKHYWNFMVELLPRWLAPNAVTLVGFGFILTNVICLEIWLPDLVGPGPSWLYYSFAFGLWAYSTMDNIDGKQARRTGSSSPLGELFDHGIDSLNCTLASLCETACMGLGPTRAGVFTALIPTLPMFFSTWETYHSHVLYLGYFNGPTEGLIIACSLMVMSGYYGPEIWTYRISDVFGHEWLFEDYSIRDIWIPVILGSFLLAHLPFCIYNVVIARRRQKLPVLPVFLEWTPMVVFTLSCGLWLYSPHTTLLEENRLVLFCLTMSFVFGRMTTKIILAHLTHQPFPYWTVLLAPLIGGAVLANLPSFGLAPISSFVELWYLRGYFVFAAIVYFRWAMLVIDGICGYLGIQCLSIPYTPASRPRVLNGAAHPLSPKKERAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJG6</accession>
    <name>D5GJG6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00008990001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430330">
        <property type="protein sequence ID" value="CAZ84659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840468.1">
        <property type="nucleotide sequence ID" value="XM_002840422.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJG6"/>
    <dbReference type="FunCoup" id="D5GJG6">
        <property type="interactions" value="86"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJG6"/>
    <dbReference type="GeneID" id="9182660"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008990001"/>
    <dbReference type="eggNOG" id="KOG0874">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043293_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJG6"/>
    <dbReference type="OMA" id="FFIFWDR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102772">
        <property type="term" value="F:sphingolipid C4-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042284">
        <property type="term" value="F:sphingolipid delta-4 desaturase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051999">
        <property type="term" value="P:mannosyl-inositol phosphorylceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050307">
        <property type="entry name" value="Sterol_Desaturase_Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11863">
        <property type="entry name" value="STEROL DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="131"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid hydroxylase" evidence="7">
        <location>
            <begin position="143"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="332"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ84659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="389" mass="44599" checksum="F909AE27ADDC3362" modified="2010-06-15" version="1">MNLTNSTTSPSPPASFLRPLPPLISGVPDSIVLVILPIAAYWALSLFFHFLDVHDLLASYRLHTPEEVLKRNHVTRREVVRDVIVQQIIQTIVGLAIGVSEPEEMIGGEWWEIMGLYSKFMGDWRYKLVEIVYWYLLPAARFWVAIFIMDTWQYFLHRLMHEVKWLYKTFHSRHHRLYVPYAFGALYNHPFEGLLMDTIGAGIAYKISGLRIRGAMTFFTFSTMKTVDDHCGYALPFDPLQYFFWNNAGYHDVHHQGWGIKTNFSQPFFICWDRWLGTQWTGGDVSARYASSREKAAEEPEKDGIIPEQKGNITATIPQKGANSTAYMSSGRTNGLKPRIPNGKPKVQADESRRRVLDTDCLGVSREEGNEETAVGNVRYWARVNGSVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJR6</accession>
    <name>D5GJR6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00009153001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430333">
        <property type="protein sequence ID" value="CAZ84759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002840522.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJR6"/>
    <dbReference type="STRING" id="656061.D5GJR6"/>
    <dbReference type="GeneID" id="9186829"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009153001"/>
    <dbReference type="eggNOG" id="KOG4575">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016738_1_1_1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="CAP/Cytokinesis_protein"/>
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    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002931">
        <property type="entry name" value="Transglutaminase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46333">
        <property type="entry name" value="CYTOKINESIS PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46333:SF5">
        <property type="entry name" value="TRANSGLUTAMINASE-LIKE DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
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        <property type="entry name" value="Transglut_core"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="TGc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cysteine proteinases"/>
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            <begin position="305"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="220"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="30"/>
        </location>
    </feature>
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            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
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        <location>
            <begin position="60"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
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            <begin position="132"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
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            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
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            <begin position="199"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00460"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAZ84759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="565" mass="62050" checksum="4C39126951A9BEAA" modified="2010-06-15" version="1">MNPSIESTSGGSIRERMAMLQLNQVRRNASTPPTRVGPPAPPPLPQKRVPPPLPTRKMSRSSLASCSSSSSSSGSDGGSITTIGSNPPGRSLPPPYTGEESLPVQFAEKPKVHEQLTKTPPSLPARSKTAPVLPPRGLPPPPRGPPLPSRANMSPTRNQERPQPIPRRLPPPPSSSVPPLPNRLKHPAESHPEPISSRSPPPIPRSTRPPPVPLSTRPDLSSCLSPARPCLVCYDFSGPDKHASLPQFERTRVGSLQQLAIGLISPFSSTLDRARTIFTWLHYNVSYDVHGFLNGKMASQDPEGVLQSGTAVCAGYAGLFNKLATYAGLESKVVSGHGKGYGFSPGEPYKTNHAWSAVKMDWGWHLIDCCWGAGSINGPPNPGYNKRLAPEHFISPPNVFGRRHFPEDSSWQCREDGRIIDWEEYMTPDQEEPEPLRYLPFGEEFGFSEKAVQPPVNNIDAFVGKKTVFSLGLPCEHMSRKEEWVLFLVIGEFDDENWMLMDSDGRGRYQIEVQLPNQRGLKVGLYHAVTWKGNDAKGLSAKEWQAEVGKVAWSWCPIIIWGADK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKD9</accession>
    <name>D5GKD9_TUBMM</name>
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        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
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        <name evidence="2" type="ORF">GSTUM_00009495001</name>
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        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
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            <taxon>Pezizomycetes</taxon>
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            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430338">
        <property type="protein sequence ID" value="CAZ84982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002840745.1"/>
    </dbReference>
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    <dbReference type="KEGG" id="tml:GSTUM_00009495001"/>
    <dbReference type="HOGENOM" id="CLU_2851355_0_0_1"/>
    <dbReference type="InParanoid" id="D5GKD9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="65" mass="7629" checksum="7A3B93BB1D39E557" modified="2010-06-15" version="1">MPRNGKSRLDSFHPPRTTIGTTPLQVRVQHGKTKAPPQMQYVYSSPIAYKHARRVLRPQNYFVWH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKL2</accession>
    <name>D5GKL2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00009626001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
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            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_002840818.1"/>
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    <dbReference type="FunCoup" id="D5GKL2">
        <property type="interactions" value="957"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GKL2"/>
    <dbReference type="GeneID" id="9184717"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009626001"/>
    <dbReference type="eggNOG" id="KOG0928">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001204_3_1_1"/>
    <dbReference type="InParanoid" id="D5GKL2"/>
    <dbReference type="OMA" id="CRDIRHH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Arf Nucleotide-binding Site Opener,domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056604">
        <property type="entry name" value="GBF1-like_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032691">
        <property type="entry name" value="Mon2/Sec7/BIG1-like_HUS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023394">
        <property type="entry name" value="Sec7_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000904">
        <property type="entry name" value="Sec7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035999">
        <property type="entry name" value="Sec7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663:SF388">
        <property type="entry name" value="GOLGI-SPECIFIC BREFELDIN A-RESISTANCE GUANINE NUCLEOTIDE EXCHANGE FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663">
        <property type="entry name" value="GUANYL-NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01369">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12783">
        <property type="entry name" value="Sec7-like_HUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23325">
        <property type="entry name" value="TPR_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00222">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48425">
        <property type="entry name" value="Sec7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50190">
        <property type="entry name" value="SEC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SEC7" evidence="2">
        <location>
            <begin position="521"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1437"/>
            <end position="1473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1450"/>
            <end position="1471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ85055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1486" mass="164382" checksum="DABDD5191995C8B6" modified="2010-06-15" version="1">MEEEELPTLSTPQKISVAINPIALVTTECISVTSAMRKNARWAQSSVSVILGGGGGSVASGGSGSATGSGLSTPIPGSPVRGLQRRKLGGEEVGLDMGLTSRWGLRGKRGKSIQDNPLMAAFAKLRSDLQDCKDIRRFDTPSLLHPFLQVIRSSSTSGPITSLALIAVTKFFAYSLINRDSPRLSLAMQLLSSAITHCRFEASDSAQDEVVLLRILKLMEMMISGPGGELLGDESVCEMMETGLSMCCQMRLSEMLRRSAEMSMVFMCQVVFERLKHLEIEADVLGGDLEESSKDEMESVAEEEQEVKPYSLPSIRELLRVLVELLDPHNKTHTDTMRVMAMRIIDVAFEVAGPSIAKHPSLASLAKDDLCRYLFQLVRSDTMTILQESLRVTGTLLATTRSVLKLQQELFLSYERQKLGMEGGSRRPDAREAMVESVGALARIPSFMVELYVNYDCEVDRSDLCEDVISFLSRNAFPDSATWSTTNVPPLCLDALLGYIGFISERLNNEPAAEGYPDPGKLQKQRARKQIIINGANKFNEDPKKGIQYLVAENIIDRADNPDSIARFLKGTSRINKKLLGEYLSKKSNMNILTVFMEMFDFSGKRVDEALRDMLETFRLPGESALIERIVTVFSEKYCSGVKPDDVEDKDAVFVLSYAIIMLNTDQHNPNLKSQARMKYEDFARNLRGVNGGKNFEPAYLQAIFDTIKNNEIILPEEHDNKHAFDYAWRELLLKTMSAGDLVICDTNIYDADMFAATWKPIIATLSYVFLSATDDAVFSRVIAGFDQCARVAAKYGLTEALDHIVRCLSTISTLSTETPPSTALNTEIQVNNNSVMVSELAVKFGRDFKAQLATVVLFRVVTSNEIVLSEGWKQIVRIWLNLFVNSLIPPFFSQSESGLDISSIPLQTPSVVIERSQTSKEAGLFSALSSYLSSYASDEPPEPSDEELDSTLCTVDCINACYLGDIFVNIMQLDATNLGSLVTALLSQLPNLDEDEDDSANIVIIKPDYPAQSPNGSNPVKKGPIYDPAVVYVLELATCLVLRDEETVAEHGKMLAETLTNIVRNAGLTHQIVLSRVIYYLFSLLQASHNRSFLNVPVLLHSIATLDKGLLAKSASPAVKGLSKCVKSASSSLKTEMVNSPDFWVLLRILLPNADASAEAFSVMELITSESPYVTSDNYVPVVSLLNDFASAGSVGSIFEQKQDKLLKRGKAKKIPSDAEIVARGAKAVLMVYQLTPRVPNLIKQSHLEKKEAWTTYWSPILESLSTQCINPCREIRNQAFSSLQPSLLSPDLTSDDHHEWTAIFGDVLFPLINRLLKPEVFQSDPRGMSDTRVQAATLLCKVFLHYLVMLSEWDGMLDLWLKILDIMDRLMNSGQGDHLEEAVPESLKNILLVMASGKYLVPPEEGGKNTELWNLTWKRLERFLPDLHEELFRAPSPLPELGSATAGTDEKKVEEGREEAKPRSSEEAKTTVATVATMALEDID</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKN3</accession>
    <name>D5GKN3_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Patatin-like phospholipase domain-containing protein</fullName>
            <ecNumber evidence="7">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00009668001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Lipid hydrolase.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Probable lipid hydrolase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Membrane</location>
            <topology evidence="7">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the PLPL family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="7"/>
    <dbReference type="EMBL" id="FN430340">
        <property type="protein sequence ID" value="CAZ85076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840885.1">
        <property type="nucleotide sequence ID" value="XM_002840839.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKN3"/>
    <dbReference type="GeneID" id="9184738"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009668001"/>
    <dbReference type="eggNOG" id="KOG2214">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009031_2_0_1"/>
    <dbReference type="InParanoid" id="D5GKN3"/>
    <dbReference type="OMA" id="CSWFTRG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990748">
        <property type="term" value="P:cellular detoxification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006642">
        <property type="term" value="P:triglyceride mobilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07232">
        <property type="entry name" value="Pat_PLPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050301">
        <property type="entry name" value="NTE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021771">
        <property type="entry name" value="Triacylglycerol_lipase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226">
        <property type="entry name" value="NEUROPATHY TARGET ESTERASE/SWISS CHEESE D.MELANOGASTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226:SF66">
        <property type="entry name" value="TRIACYLGLYCEROL LIPASE PTL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11815">
        <property type="entry name" value="DUF3336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6 7" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="5 6 7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="PNPLA" evidence="9">
        <location>
            <begin position="271"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="621"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="6">
        <location>
            <begin position="302"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="304"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ85076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="718" mass="82145" checksum="B35248ABF92AF9FA" modified="2010-06-15" version="1">MAGWDRSNEPRRRKQERGGEEGLPNFDTDFVNEADIAAFERAINAPDATDEDFITALNDWKPIKQRIRRKRPKMRPRRGKDETREGFVYQLLKWPLLTIVFAWILILSILYTMTRFYVSQYEYWVTWRGRRERLRRKLRSTKTYEEWKLAAKELDSYLGADTWKAEDAFAYYDYSTIRKAVANICRLRQKAEEEERAGGTLVKACVKSNFAGIESFRMYSQTYYGTKDRVQEFIRELEKSLKFLLETNSLDMEEKRVLFKHLSSNYGRTALCLSGGASFAYYHFGVVKAHLDAGLLPTVITGTSGGALVAALVCTRTDEELKKLLVPALALKITACHDDTLTWLRRWWKTGARFDSVDWARRLCWFTRGSLTFKEAYERTGRILNISCIPSDPHSPSLLLNYLTAPDCCIFSAVLASAAVPGILNPVVLMTKTPHHPFTITPFSFGHKWKDGSLRTDIPLRALNTHFNVTFSIVSQVNPHVNIFFFSPRGSVGRPVTHRKGKGWRGGFLGSALEQYLKLDMAKWLKVLRHLELLPRPLSQDWSSVFLQKFDGNITIWPRTRVSDFWYILSDPTVERLRRMLEVGQRCTFPKLEFVRNRLAVERVVERGRRITRGAGKAELVAGGQVRGRKPSPLPLPSPRGEEKGQEKKSFNWFHWPSPTGRSTNVDGNHSNTSVLEEMENHERVFFDDDYGGSSGSVSSDEEAMEKFEAAGIAGGGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLF6</accession>
    <name>D5GLF6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00010173001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430348">
        <property type="protein sequence ID" value="CAZ85349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841158.1">
        <property type="nucleotide sequence ID" value="XM_002841112.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLF6"/>
    <dbReference type="FunCoup" id="D5GLF6">
        <property type="interactions" value="68"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GLF6"/>
    <dbReference type="GeneID" id="9186093"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010173001"/>
    <dbReference type="eggNOG" id="KOG4375">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021546_0_0_1"/>
    <dbReference type="InParanoid" id="D5GLF6"/>
    <dbReference type="OMA" id="DWTAEEC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035255">
        <property type="term" value="F:ionotropic glutamate receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030160">
        <property type="term" value="F:synaptic receptor adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01786">
        <property type="entry name" value="RA_STE50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09533">
        <property type="entry name" value="SAM_Ste50-like_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051569">
        <property type="entry name" value="SHANK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24135:SF28">
        <property type="entry name" value="LD13733P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24135">
        <property type="entry name" value="SH3 AND MULTIPLE ANKYRIN REPEAT DOMAINS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00788">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00314">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAM" evidence="2">
        <location>
            <begin position="51"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-associating" evidence="3">
        <location>
            <begin position="326"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="397"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="220"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="310"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="401"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ85349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="439" mass="48663" checksum="776B96F4F4579764" modified="2010-06-15" version="1">MPSPGHHQQPSSPTDSDIPSRDGDQHTPTFHQPHYHEMDNRDMSDRHILEWDAEDVAGFIRSLVLDQYGDSFLDNEIDGQVLVHLDHEELRDVGVQSVGHRLTILKNVYNIKIAHGVSIDTGDYVPVSADVEDTEKSATQHDIQRLIGIIRQRDERISQTEGELRELQKGITKLREELLPVWRRERDKRDRDRDREERDRERDQKLILGTPKNPSPTHSTHPENVRTDYEPPPTATNGNATIANLQNSQSPGNIPSPTSPSTIYHVPGTGVGNPLNNSNLGGMNAPAATHRPADGQPPRTRPPRSDYEEPNPLSTPSNSSSSVEIFKSFRVSMEDPCYKVLPAALKRYNIQANWRQYALYIVHGDQERCLGLEEKPLILFKQLDREGRKPMFMLRRNGNFDSGGGSSNGGGPSGGGIGGSGASVYGGREGGITLPGGVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLP9</accession>
    <name>D5GLP9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00010307001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430350">
        <property type="protein sequence ID" value="CAZ85442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841251.1">
        <property type="nucleotide sequence ID" value="XM_002841205.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLP9"/>
    <dbReference type="GeneID" id="9182745"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010307001"/>
    <dbReference type="HOGENOM" id="CLU_1020110_0_0_1"/>
    <dbReference type="InParanoid" id="D5GLP9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ85442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="273" mass="29198" checksum="42686243E1C836C4" modified="2010-06-15" version="1">MRYLGSSTIGELVADSSIFPCSEKGWAGILLHHGWPEDSYTDSIGGAQGFMALATVDVYITIPPTCIYRWCAHFAEVIVKAHLHEIQGTEPVVHPPDFRSTPPHSPHQYNSGSLKHPIEHLQLLNLANQGLLSCGTGIVETKDPSTFVTPTAAWEVIMNADAASHALEKEVPGIRYYSNSVVAASIADAEVGAPSWGRAGSIIAAWPQTVAEACRATSSGEGFQPEKERLVFAHAKLSMLRASYVTIMLKAAGSVGPGLEEDSELVTGLAYMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5GM43</accession>
    <name>D5GM43_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00010519001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFB1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430352">
        <property type="protein sequence ID" value="CAZ85586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841395.1">
        <property type="nucleotide sequence ID" value="XM_002841349.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GM43"/>
    <dbReference type="FunCoup" id="D5GM43">
        <property type="interactions" value="909"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GM43"/>
    <dbReference type="GeneID" id="9187370"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010519001"/>
    <dbReference type="eggNOG" id="KOG2074">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019188_1_0_1"/>
    <dbReference type="InParanoid" id="D5GM43"/>
    <dbReference type="OMA" id="VCTCELL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13229">
        <property type="entry name" value="PH_TFIIH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005607">
        <property type="entry name" value="BSD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035925">
        <property type="entry name" value="BSD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027079">
        <property type="entry name" value="Tfb1/GTF2H1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013876">
        <property type="entry name" value="TFIIH_BTF_p62_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12856">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03909">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08567">
        <property type="entry name" value="PH_TFIIH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00751">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140383">
        <property type="entry name" value="BSD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50858">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BSD" evidence="8">
        <location>
            <begin position="141"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="BSD" evidence="8">
        <location>
            <begin position="215"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="104"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="555"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="111"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ85586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="616" mass="67726" checksum="841BC26AC26FEF94" modified="2010-06-15" version="1">MASIAKGSASYKKKDGSLVLEKDSVTWTPANAETPSLKIPVSTITNLQATPATVAKVMIKVFATKEGGAAEPYVFTFTSASSARMEADVMKEALTTSIQTFKNNSGTATPSSASVGHTTTAASSTAASGATSAKLASKLTDWSKTRLETDLELQKSLLKADQELSTTFSEAVLSGAVTADQFWSTRTHLLRAHAIEREQKRGPYNVLATIKSKTVDNVVKMSLSREAIHDIFDQHPLVKMVYDDNVPKLPEEEFWSRFFLSRLFKKLKGDKLLPTDNTDPILDRYLGRGDEENSRKRRKLEHVPRTMDIEGNEQNLSQKKGNAPDLTMRPTRTENVPIIKTLNSLSLKLVDLVTPTDTDPSQVEDQDERYIIEQRLIDLHSDPEEARIILNIRDQRQFFSASSNADKARKELYSNLKPETVLQEIQKTLGESNLNLLTALASGGTSKGSKAQLSKATTQIVAAVKDRREQMLNSSGFKNKSGGLPANVFNSVTLVHATTNEFLRHFWLAFLSGDEKRAGDIVKLVNSLKNSKERIASIAKTADEERELEKVRKKKEAQEEYKRTGVKPKRKTTEVGGGSKVVEELLAPTIVAVDKALGKYQAALGKADKGSRTAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMG8</accession>
    <name>D5GMG8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00010700001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430354">
        <property type="protein sequence ID" value="CAZ85711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMG8"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010700001"/>
    <dbReference type="HOGENOM" id="CLU_3419477_0_0_1"/>
    <dbReference type="InParanoid" id="D5GMG8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="25" mass="2925" checksum="091FB6E52784EABB" modified="2010-06-15" version="1">MDQEATKQVPQHMIRNEDQTNKIGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMM2</accession>
    <name>D5GMM2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">DNA replication complex GINS protein PSF2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00010810001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">The GINS complex plays an essential role in the initiation of DNA replication. Has a role in chromosome segregation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the GINS complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the GINS2/PSF2 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430357">
        <property type="protein sequence ID" value="CAZ85765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841574.1">
        <property type="nucleotide sequence ID" value="XM_002841528.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMM2"/>
    <dbReference type="FunCoup" id="D5GMM2">
        <property type="interactions" value="657"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GMM2"/>
    <dbReference type="GeneID" id="9185101"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010810001"/>
    <dbReference type="eggNOG" id="KOG4071">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078274_1_1_1"/>
    <dbReference type="InParanoid" id="D5GMM2"/>
    <dbReference type="OMA" id="DSLNCMY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071162">
        <property type="term" value="C:CMG complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000811">
        <property type="term" value="C:GINS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033260">
        <property type="term" value="P:nuclear DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd11712">
        <property type="entry name" value="GINS_A_psf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21694">
        <property type="entry name" value="GINS_B_Psf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.5.50:FF:000001">
        <property type="entry name" value="DNA replication complex GINS protein PSF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021151">
        <property type="entry name" value="GINS_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036224">
        <property type="entry name" value="GINS_bundle-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007257">
        <property type="entry name" value="GINS_Psf2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056784">
        <property type="entry name" value="PSF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12772">
        <property type="entry name" value="DNA REPLICATION COMPLEX GINS PROTEIN PSF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12772:SF0">
        <property type="entry name" value="DNA REPLICATION COMPLEX GINS PROTEIN PSF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25005">
        <property type="entry name" value="PSF2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05916">
        <property type="entry name" value="Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028998">
        <property type="entry name" value="GINS_Psf2_subgr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158573">
        <property type="entry name" value="GINS helical bundle-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160059">
        <property type="entry name" value="PriA/YqbF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="4 8" id="KW-0235">DNA replication</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="186"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="DNA replication complex GINS protein PSF2 N-terminal" evidence="11">
        <location>
            <begin position="13"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="GINS subunit" evidence="10">
        <location>
            <begin position="76"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR028998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF25005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ85765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="211" mass="23619" checksum="817FA4ECEB2AD379" modified="2010-06-15" version="1">MNNNNHSASGPLTPAEVAFLCEAQSVTIIPRQRLDGLDLIGGYIPPFHPPQRTTIPLWLALLLKRQRRCNIVPPPWLSAPNIERILKTETENQNLFCPDLPYRWLETAELLLEGCPDDLGGDDGELRVLLRGLREVRQGKVREGLGKLEATYLQMNNLGLLEITEVRGFAKGVVDGLRKIGASTGVGLVLGWMMMISRWGWCFISMVFWTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMP5</accession>
    <name>D5GMP5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00010867001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TfdA dioxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430358">
        <property type="protein sequence ID" value="CAZ85788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841597.1">
        <property type="nucleotide sequence ID" value="XM_002841551.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMP5"/>
    <dbReference type="GeneID" id="9185124"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010867001"/>
    <dbReference type="eggNOG" id="ENOG502QT05">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036005_0_1_1"/>
    <dbReference type="InParanoid" id="D5GMP5"/>
    <dbReference type="OMA" id="SSHMQNY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016706">
        <property type="term" value="F:2-oxoglutarate-dependent dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.130.10:FF:000003">
        <property type="entry name" value="Alpha-ketoglutarate-dependent taurine dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.10">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051323">
        <property type="entry name" value="AtsK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042098">
        <property type="entry name" value="TauD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003819">
        <property type="entry name" value="TauD/TfdA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30468">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT SULFONATE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30468:SF31">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT SULFONATE DIOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02668">
        <property type="entry name" value="TauD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TauD/TfdA-like" evidence="8">
        <location>
            <begin position="85"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="355"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="367"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="379"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ85788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="393" mass="44031" checksum="9B22C0F07A881779" modified="2010-06-15" version="1">MAPSVVEIPTPHKVPSINGTGLEKVPRFTPGYTAVEEVTTDYKYEHLKPCFPDVSWPPLEEVPYSDKGVLGDPEYKNLLAEATDVFDYTPKIGTEIHGVKLKTLTDAQKNDLARLVAYRGVVFFRDQGDFEVGDQLDLGRYWGPLHKHATTSVPRKEGLDEIHVVYHDANGLDQRALFTPTHLWHADVTYELQPPSYTLLKVLHGPPRGGGGDTLWSSQYAAYDVLSAPMQKYLEGLTALHSAEEQAIGSRATGRPVRREPVITEHPLVRTHPVTGWKSLFVNPGFVKQIVGVPKMESDAILKYLNDIVVATQELHARFRWEENTIAIWDNRICNHTATYGFSPHMRHAIRVTPHGERPVFDPNSKSQEEDLDGKAGRTSINKNGARPSNYND</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMV6</accession>
    <name>D5GMV6_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Trafficking protein particle complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00010953001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Plays a key role in the late stages of endoplasmic reticulum to Golgi traffic.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Part of the multisubunit TRAPP (transport protein particle) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Golgi apparatus</location>
            <location evidence="7">cis-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the TRAPP small subunits family. BET3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430360">
        <property type="protein sequence ID" value="CAZ85849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841658.1">
        <property type="nucleotide sequence ID" value="XM_002841612.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMV6"/>
    <dbReference type="FunCoup" id="D5GMV6">
        <property type="interactions" value="346"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GMV6"/>
    <dbReference type="GeneID" id="9183417"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010953001"/>
    <dbReference type="eggNOG" id="KOG3315">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073154_2_1_1"/>
    <dbReference type="InParanoid" id="D5GMV6"/>
    <dbReference type="OMA" id="YMVKFDD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990070">
        <property type="term" value="C:TRAPPI protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990071">
        <property type="term" value="C:TRAPPII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990072">
        <property type="term" value="C:TRAPPIII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14943">
        <property type="entry name" value="TRAPPC5_Trs31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1380.20:FF:000002">
        <property type="entry name" value="Trafficking protein particle complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1380.20">
        <property type="entry name" value="Trafficking protein particle complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016696">
        <property type="entry name" value="TRAPP-I_su5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007194">
        <property type="entry name" value="TRAPP_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20902">
        <property type="entry name" value="41-2 PROTEIN ANTIGEN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20902:SF0">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04051">
        <property type="entry name" value="TRAPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017479">
        <property type="entry name" value="TRAPP_I_complex_Trs31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="Ligand-binding domain in the NO signalling and Golgi transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 7" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="6 7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR017479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ85849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="234" mass="26248" checksum="F2886800E0DE4B15" modified="2010-06-15" version="1">MQRGDSLNSPFVPFSPNPSIQQGTPTSTPPLKHTLSSSTSSNLRYSSSRKSIYDRHLNRSQRSELSKASFAFLFGEMVQYAQKRVNGIADLEKKLNLHGYSIGQRLLELLLWREGRSAKRETRILGILQFITTTVYRSIFNKPADGLEKSRDNEDEYMLIDNDPMVNSYISVPKEMSQLNCAAFVAGIIEAVLDGCLFPGRVTAHSVPTEQLPAKTVFLIKFDESVIERESVLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMX9</accession>
    <name>D5GMX9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00010989001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430360">
        <property type="protein sequence ID" value="CAZ85872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841681.1">
        <property type="nucleotide sequence ID" value="XM_002841635.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMX9"/>
    <dbReference type="GeneID" id="9183440"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010989001"/>
    <dbReference type="HOGENOM" id="CLU_1688016_0_0_1"/>
    <dbReference type="InParanoid" id="D5GMX9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="39"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="156" mass="16675" checksum="E9CCF79DCA2D66FE" modified="2010-06-15" version="1">MQTPPAPCLQLFQPLPHSSSPYNTLLPPLPVRVLEYGTPSPSSSPYSASSPPPRSPPTSPPPSLPLLLLPLPFLSLLPFCSRCCICITFPFNLPSPVFFSFSFHSPPPLNISLTSPSAPFLPSPDLLPALPLPAYPPISPVPSSPLSHQASAWYSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>D5GN71</accession>
    <name>D5GN71_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00011120001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430362">
        <property type="protein sequence ID" value="CAZ85964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GN71"/>
    <dbReference type="STRING" id="656061.D5GN71"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011120001"/>
    <dbReference type="HOGENOM" id="CLU_940705_0_0_1"/>
    <dbReference type="InParanoid" id="D5GN71"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="26"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="88"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="127"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="183"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="296" mass="32107" checksum="411D124B65DBE7FA" modified="2010-06-15" version="1">MPHSTFHTSSPSEVVSTTQEGLTIASSTPSTSPTQTHAQAQAQGVVATPVAIGISHQHPHLLHLHSHHHHQNIHTSKQVKKRLHMRSKSTSTTTTTTLSSHSSFSSPFYHHHHQNNPQTSTTPRIFASPLLRSSSLPLAPGNSSSPPTTPRTLWMRAYPPPSPIRTSTPSPPPPSLVNEPFVSSSSSSITSSSSPSTPTSLRSRSPSISSLETIPDSPDAEEEEEKAVEALKEEWDSRRRNTPSRSASADKRKRWSVCGAEKRSDLELETIWEEASFGVEIVGRGSCDGMGEGEMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>D5GN86</accession>
    <name>D5GN86_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">FACT complex subunit POB3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00011156001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the SSRP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430362">
        <property type="protein sequence ID" value="CAZ85979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841788.1">
        <property type="nucleotide sequence ID" value="XM_002841742.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GN86"/>
    <dbReference type="FunCoup" id="D5GN86">
        <property type="interactions" value="967"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GN86"/>
    <dbReference type="GeneID" id="9185905"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011156001"/>
    <dbReference type="eggNOG" id="KOG0526">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017374_3_0_1"/>
    <dbReference type="InParanoid" id="D5GN86"/>
    <dbReference type="OMA" id="QVVTKIF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035101">
        <property type="term" value="C:FACT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006335">
        <property type="term" value="P:DNA replication-dependent chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034728">
        <property type="term" value="P:nucleosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031508">
        <property type="term" value="P:pericentric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045899">
        <property type="term" value="P:positive regulation of RNA polymerase II transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030466">
        <property type="term" value="P:silent mating-type cassette heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd13230">
        <property type="entry name" value="PH1_SSRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13231">
        <property type="entry name" value="PH2_SSRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.220:FF:000003">
        <property type="entry name" value="FACT complex subunit POB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000146">
        <property type="entry name" value="FACT complex subunit POB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.150:FF:000001">
        <property type="entry name" value="Fact complex subunit ssrp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.220">
        <property type="entry name" value="Structure-specific recognition protein (SSRP1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013719">
        <property type="entry name" value="RTT106/SPT16-like_middle_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050454">
        <property type="entry name" value="RTT106/SSRP1_HistChap/FACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048993">
        <property type="entry name" value="SSRP1-like_PH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000969">
        <property type="entry name" value="SSRP1/POB3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035417">
        <property type="entry name" value="SSRP1/POB3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024954">
        <property type="entry name" value="SSRP1_DD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038167">
        <property type="entry name" value="SSRP1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45849">
        <property type="entry name" value="FACT COMPLEX SUBUNIT SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45849:SF1">
        <property type="entry name" value="FACT COMPLEX SUBUNIT SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21103">
        <property type="entry name" value="PH1_SSRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17292">
        <property type="entry name" value="POB3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08512">
        <property type="entry name" value="Rttp106-like_middle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03531">
        <property type="entry name" value="SSrecog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00887">
        <property type="entry name" value="SSRCOGNITION"/>
    </dbReference>
    <dbReference type="SMART" id="SM01287">
        <property type="entry name" value="Rtt106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-0158">Chromosome</keyword>
    <keyword evidence="4 10" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 10" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone chaperone RTT106/FACT complex subunit SPT16-like middle" evidence="12">
        <location>
            <begin position="370"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="479"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="498"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="530"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="550"/>
            <end position="561"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ85979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="570" mass="64081" checksum="AB79696A274CEBDB" modified="2010-06-15" version="1">MPSDIETFENIFLDLSRTPGRFRFAPSGMGWRPTTGEIWKMANTELSTAQWSRAARGYELKLFTRTMGIVQLDGFELDDLDRVKECLKAYYKVNLEHREHALKGWNWGKAEFSKSELYFNVANKPAFEIPFAEVSNSNLAGKNEVAVEFATADAGGGGTNEAGNKKKKKAAAQLDQLVEMRFYVPGTVKKGDEEAGSDAEGEEKEMSAANLFYDTLKEKADIGEVAGDTYATFLDILFLTPRGRYDIDMYESSFRLRGKTYDYKIQFENVKKFFLLPKPDDVHNMIVIGLDPPLRQGQTKYPFLVMQFKREEDMEFDLNITEDLLNERYAGKLQMHYENPAHQVVSQIFKGLTGKRIITPSRDFSSHHQQSGVKCSLKANEGHLFCLDKAFIFVPKPAVFITFDKIAHITMSRVGGSVSASRTFDVTVSLKSSEGGGEYQFNNINREEQSSLEDFFKSKNLRIKNDLVDDSSALLAQALKDQDMDDDDDVAAARGSADEDEESVDEDFQAESESDVAEEYDSAHESSGGSDDEDGPGGEEDEDEDEEMRDVEVEVREDGPAKKKVKTSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNU5</accession>
    <name>D5GNU5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Synaptobrevin homolog YKT6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00011500001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endomembrane system</location>
            <topology evidence="8">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the synaptobrevin family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430369">
        <property type="protein sequence ID" value="CAZ86188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841997.1">
        <property type="nucleotide sequence ID" value="XM_002841951.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNU5"/>
    <dbReference type="STRING" id="656061.D5GNU5"/>
    <dbReference type="GeneID" id="9182822"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011500001"/>
    <dbReference type="eggNOG" id="KOG0859">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064620_1_1_1"/>
    <dbReference type="InParanoid" id="D5GNU5"/>
    <dbReference type="OMA" id="NTKLMIM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14824">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000004">
        <property type="entry name" value="Vesicle-associated membrane protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.50">
        <property type="entry name" value="Longin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010908">
        <property type="entry name" value="Longin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001388">
        <property type="entry name" value="Synaptobrevin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051097">
        <property type="entry name" value="Synaptobrevin-like_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21136">
        <property type="entry name" value="SNARE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21136:SF168">
        <property type="entry name" value="VESICLE-ASSOCIATED MEMBRANE PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13774">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00219">
        <property type="entry name" value="SYNAPTOBREVN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01270">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50859">
        <property type="entry name" value="LONGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00417">
        <property type="entry name" value="SYNAPTOBREVIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="208"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="Longin" evidence="11">
        <location>
            <begin position="10"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="12">
        <location>
            <begin position="144"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00026133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ86188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="238" mass="25984" checksum="CCD2C8008E5DB4E9" modified="2010-06-15" version="1">MSSSSSSTPLLYACVAHGTTILSEYSPSGAASTNASSLASLILPKITHTSPQKLTYTHGQHNIHYIATASSQSTPFGQTFLVISAAHIGRKLPFGYLLEIQRLFTSKYPASAVADAPPYGCAEFNSTLGATMRQFEAGGENEDVVAKAQREIESARGIMTENIERVLERGERIDLLVDKTAGLQSGAMEFRVRSRGLRRRMWWKNTKLMILLVFVVIFLVYLFVGMGCGLPGWSRCVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNV8</accession>
    <name>D5GNV8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00011532001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430370">
        <property type="protein sequence ID" value="CAZ86201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842010.1">
        <property type="nucleotide sequence ID" value="XM_002841964.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNV8"/>
    <dbReference type="STRING" id="656061.D5GNV8"/>
    <dbReference type="GeneID" id="9182835"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011532001"/>
    <dbReference type="eggNOG" id="KOG1902">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011694_2_1_1"/>
    <dbReference type="InParanoid" id="D5GNV8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990247">
        <property type="term" value="F:N6-methyladenosine-containing RNA reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21134">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.590.10">
        <property type="entry name" value="ph1033 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057720">
        <property type="entry name" value="RRM_YTH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007275">
        <property type="entry name" value="YTH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045168">
        <property type="entry name" value="YTH_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357:SF3">
        <property type="entry name" value="YTH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357">
        <property type="entry name" value="YTH YT521-B HOMOLOGY DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25701">
        <property type="entry name" value="RRM_YTH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04146">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50882">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="405"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="YTH" evidence="4">
        <location>
            <begin position="529"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="89"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="254"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="355"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="490"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="731"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="8"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="89"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="254"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="355"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="377"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="499"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="734"/>
            <end position="744"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ86201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="750" mass="81557" checksum="DF3D857EC8FBFFDA" modified="2010-06-15" version="1">MGDVQPPNELTRNLIVSSTPPGGYTAQEQTVAPERQPPATLPKNPDPYTGIPSHPLAGDMGMAAPLVQQSQGYRLNQPQYVQVPPLLSQRSHSTPHHQPTQFMHNGAHVPQSLHPHPQMSQQGTHIPPTNQRWQTGHSPAGIAYQLSQQTQQYQRGPASPTPNGQYFVPGYQHTFIDSYGQQYTQVLTQHRDPQAQVQSGSCAVCYSSSYPPHHPQSTGPTQTQFQHWYGPQMSPYYPVPPQYIVHQVPVQHLPQPPYSQAPPGPYVRRHSFPLRQLPPKPRESDSGTARTSRFAGHPGMSGCDGRAAKAAAVGSGIGASGREEAAVGGYAEPSARGPGQSYCPYPTLPLLQPALSQSNSSAETSGISTNATTPGALDSSLSSSLSLPHPSLPRGPPRKPKQSGHALWVGNLPTGAQVLDLKEYFSKDAKSDIESVFLISKSNCAFVNYRTEEACSEAMARFHDSRRNSAPSAAGGAMALALSLDPSAQSIQDAQDVQDSGDEKEEDGADGVKSPPVGPKVTTRIVGKDRIFIVKSLTVEDLDLSVRNRIWATQSHNESTLNQAFETADNVYLIFSANKSGEYYGYARMTSPILDDPTGLEWTPSSQPHEDSDLPRAIYTPATSQAPQGRIIDDSARGTIFWEAISDEEVPGPIVTTNEGEDSREKGSVAVAKAWGKPFRVEWVSTTRVPFYRTRGLRNSWNANREVKIARDGTELEENVGRRLIQLFHRNGSGPVTGNQNTHMPTLRAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5GP49</accession>
    <name>D5GP49_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00011702001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430373">
        <property type="protein sequence ID" value="CAZ86314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842123.1">
        <property type="nucleotide sequence ID" value="XM_002842077.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GP49"/>
    <dbReference type="STRING" id="656061.D5GP49"/>
    <dbReference type="GeneID" id="9186554"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011702001"/>
    <dbReference type="eggNOG" id="KOG0565">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002027_1_1_1"/>
    <dbReference type="InParanoid" id="D5GP49"/>
    <dbReference type="OMA" id="GNQNAQW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004439">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000036">
        <property type="entry name" value="Inositol polyphosphate phosphatase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046985">
        <property type="entry name" value="IP5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000300">
        <property type="entry name" value="IPPc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200">
        <property type="entry name" value="INOSITOL 5-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200:SF240">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE 5-PHOSPHATASE C9G1.10C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22669">
        <property type="entry name" value="Exo_endo_phos2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00128">
        <property type="entry name" value="IPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inositol polyphosphate-related phosphatase" evidence="2">
        <location>
            <begin position="855"/>
            <end position="1199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="155"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="288"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="364"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="431"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="448"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="500"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ86314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1263" mass="139188" checksum="C1BE1F501D517F27" modified="2010-06-15" version="1">MTTSKPKPPVPSPSGSETVQNPLRPAHTGSDPFLERNSNGGGNFHVRASLDIPREFFAGSAATVGRGGVSKPTNNNGSSNAYTLPRGFAQSSRQRPLSVHSTFQSTPTHSPPSFRFDSPKTSPNSLNLDARGPSPERPSDSPKATPAASPHSRSFRVSGCSHSRSSSPSPNTEHRPPPPPPVSARPVRHEQPPPPPIAPPSGSISGDRRASMPNNGAFRRPPPSPVLQAPQDYSEPEKRTQPEHTAPPVPMRPTKPKIPSKPSIPVKPDRASSNSGLAPAASSEEHVSPFSTPPSSANNSPSRSDNESGKQPRIMSRQTTGMPTYSPPPPLHYTTMERARERNGAGVDTGILPAKAPIPPPPRISREEIRPRMKFEDSFRGQRTSLDDAGLRPDLPPRPPGGIIGGIRARVPSLRGRSYSPPRVSSELGPAPEPPTFLPPPRRGFSSVKPQTSGGQEAVASSSRISPNTSVATNSTTYSNGAEDSYGGYGPDDSDEALSDENQSALSEYPDSSQANRRHPVSKSGIRDINCRTYVRYFAVCGKYVCTTSNATNVWNMDTGQCIMSINHGESTKVTAIAFKPSVNVEDEGKQLWIGTSHGELMEVDIPSQKITNTRASAHMKKEVVQIHRCGYDLWTLDDGGKLQVWTPDVNGVPNLKNSPTTFRIPNKHTYSLVVDGMLWIGAGKNVHVYQPSSDSRVQFNVTSRPLTPNKPTAEITCGAVVNSEPDKVYFGHSDGKVSVYSRSKFTFLDVVNVSLYKINSMSGVGDYMWAGFKTGMIYVYDVRTKPWTVLKDWQSSHGPVVQVVADRTSIWKVGRLEVLSLGVDNVIRAWDGMLEDDWLEKEMQQHDVEFCSFREIKALICTWNAGANKPQDLLTREDDKVFLENVLGSVDSPDIIVFGFQELVDLEDKKITAKSLLKGGKSKKKKAADQQEHMSHQYRLWQQQLTKSIETHMPRDQPYALLHVANLVGLFTCIFVKTSERHSIRNVCASTVKRGLGGLHGNKGALVVRFLLDDSSLCFINCHLAAGQSHTISRNNDIAAILESTALPAEPSAEARTDMFVGGGDGSMILDHEICVLNGDLNYRIDMHRDSVISRERQGDLPALLERDQLLMERRRNPGFRLRAFQESQITFSPTYKYDVGTDIYDTSDKKRSPAWCDRVLYRGLGRIKQTAYRRWEVRASDHRPVSASFLIRLKTVDQELRGHVWEQCKRRYAEVREREVWEAKLDFLVNVCGYGLEEARRSLGGKEGVVEAVRSLQKSGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPK9</accession>
    <name>D5GPK9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00011895001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430376">
        <property type="protein sequence ID" value="CAZ86452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842261.1">
        <property type="nucleotide sequence ID" value="XM_002842215.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPK9"/>
    <dbReference type="GeneID" id="9186692"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011895001"/>
    <dbReference type="HOGENOM" id="CLU_1171351_0_0_1"/>
    <dbReference type="InParanoid" id="D5GPK9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ86452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="237" mass="26666" checksum="0FFC1FCD1332C41C" modified="2010-06-15" version="1">MARSRSPPLAFLSQPPLPAFNLQQPSLPCSRPHTFSSTAASPARESDAYEAIHNQYQKLLSHEREGMELERTLWLSERQFYLEKIVKFESTIKLLREELEKAKASVSGGGPRDEEGDQDPEKDNLAVITSPPPPVTGPIPGAESPPADVPIRPTEAESPEVEEAQTPKQENGVEQFARPFSELDEKTTNLLYEQARRIVYPQRTWPEEKIVLDDDGEGDDEDIKLILKKNTNFGWAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPN5</accession>
    <name>D5GPN5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00011947001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="5">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430377">
        <property type="protein sequence ID" value="CAZ86478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842287.1">
        <property type="nucleotide sequence ID" value="XM_002842241.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPN5"/>
    <dbReference type="STRING" id="656061.D5GPN5"/>
    <dbReference type="MEROPS" id="T01.P02"/>
    <dbReference type="GeneID" id="9186718"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011947001"/>
    <dbReference type="eggNOG" id="KOG0180">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_460188_0_0_1"/>
    <dbReference type="InParanoid" id="D5GPN5"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03759">
        <property type="entry name" value="proteasome_beta_type_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000003">
        <property type="entry name" value="Proteasome subunit beta type-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033811">
        <property type="entry name" value="Proteasome_beta_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF10">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA TYPE-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0647">Proteasome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="58"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="58"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="105"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ86478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="593" mass="66229" checksum="92A126DBC7BE916A" modified="2010-06-15" version="1">MESSSPEEKKPKPKMMFKKRTFDKKTENGVVKSAGPTDDISFFSRSKDTFVSAAEIRQRDLARRGKEKAEKFYSPPLTINKGSPKGKKRRSSEELSEDDEIKLGRSTNNQSVSLHSRYKNAKIILDDSDNEASPSVQKKRDRGIELTPPPCLYYEPALPRPIPKPNFQSPVISLSDSEPDLIVTSSAPVTTIDNYDDDEPQEQNPALAAIEKKARERAARMRATQSPSVESQSAFLGPCVSILVTSDIPKTKPLICRRRVGQTLKEVRLAWCQRQGFNEDTTSDVFLTWKGNKIFDVNTCTGMGISPDEEGNFEENTDGVKDKHLHFEAMTAEIFAERERSREAQYDGLEEEPEEKVEESIGIVLKSKGYANFPTKVRPEFILLQPLIMSNPFSINGGAVVAMVGKDCVAIACDLRLGMQALTVSNDFPKIFNYGDVYLGLTGLATDVTSVSELFRYKVNMYRLREERDIEPQTMANLVSSSLYEKRFGPYFVSPVIAGINQHSGKPFICGFDSIGCINFAEDFIVSGTASDQLFGTCEGLWEPDLEPEDLFETISQALLNSVDRDALSGWGAHVYLVTKDKVVKRLLKGRQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPU4</accession>
    <name>D5GPU4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">peptidyl-tRNA hydrolase</fullName>
            <ecNumber evidence="1">3.1.1.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00012036001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>an N-acyl-L-alpha-aminoacyl-tRNA + H2O = an N-acyl-L-amino acid + a tRNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54448"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:13883"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59874"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:138191"/>
            <dbReference type="EC" id="3.1.1.29"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PTH2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.29" evidence="1"/>
    <dbReference type="EMBL" id="FN430378">
        <property type="protein sequence ID" value="CAZ86537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842346.1">
        <property type="nucleotide sequence ID" value="XM_002842300.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPU4"/>
    <dbReference type="FunCoup" id="D5GPU4">
        <property type="interactions" value="941"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GPU4"/>
    <dbReference type="GeneID" id="9182281"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012036001"/>
    <dbReference type="eggNOG" id="KOG3282">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073661_0_1_1"/>
    <dbReference type="InParanoid" id="D5GPU4"/>
    <dbReference type="OMA" id="RMDLGMT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004045">
        <property type="term" value="F:peptidyl-tRNA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02430">
        <property type="entry name" value="PTH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1490.10:FF:000001">
        <property type="entry name" value="Peptidyl-tRNA hydrolase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1490.10">
        <property type="entry name" value="Bit1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023476">
        <property type="entry name" value="Pep_tRNA_hydro_II_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002833">
        <property type="entry name" value="PTH2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00283">
        <property type="entry name" value="arch_pth2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12649">
        <property type="entry name" value="PEPTIDYL-TRNA HYDROLASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12649:SF11">
        <property type="entry name" value="PEPTIDYL-TRNA HYDROLASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01981">
        <property type="entry name" value="PTH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102462">
        <property type="entry name" value="Peptidyl-tRNA hydrolase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="40"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="66"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ86537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="214" mass="22591" checksum="846D43F564998A2F" modified="2010-06-15" version="1">MPPMTSLPPPLSTIILTLLGALTGYYIGKTSGVKTAERIKELKSSPPVSPDDSFTPASPTAKPPSEDNDDDDDDWATTSSTINPYTNLSEDCKMVFVVRTDLSMTKGKIAAQCGHATLMCYKSVREHAPGILERWERLGQAKIALKCSGGEEGGLDGEEELETLAGVAASLGVVARIVRDAGRTQIKAGSATVLGVGPAPRSIVDQITGHLKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPX6</accession>
    <name>D5GPX6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00012063001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430379">
        <property type="protein sequence ID" value="CAZ86553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842362.1">
        <property type="nucleotide sequence ID" value="XM_002842316.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPX6"/>
    <dbReference type="STRING" id="656061.D5GPX6"/>
    <dbReference type="GeneID" id="9182296"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012063001"/>
    <dbReference type="eggNOG" id="ENOG502QTT6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008227_1_0_1"/>
    <dbReference type="InParanoid" id="D5GPX6"/>
    <dbReference type="OMA" id="YHEFHED"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013946">
        <property type="entry name" value="NCA2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28234">
        <property type="entry name" value="NUCLEAR CONTROL OF ATPASE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28234:SF1">
        <property type="entry name" value="NUCLEAR CONTROL OF ATPASE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08637">
        <property type="entry name" value="NCA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ86553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="675" mass="75989" checksum="CC1DC6E84EEAA748" modified="2010-06-15" version="1">MTFVSDQLLHLDAQIDRLALDRLPLTFDSHPATPHSLPSQPPRLPPPTPRAIKLQNIIKSLSTTTPRTLLSPKRLVSLLKNATIPTHTYGSYEAELYETELEWLLLSKATIQTYGIVLNSLLEQTLPLSQDLFYWDEVAGSYSYTALYSLQTAPEKMWDLGRESLLENDNQSEREDTKGKETLSATARRFYALVRNSLRDRSLVEFQRVGALSPFSLARHEIRMKQAGIKKLREMQASALGVLIGEGLSFGFEEEEHEEWKIIVERAVLLMEKVVRNVSSVESTLDEFEETVFSLDGLGGRTDDDEEDERGSRLSLRHYTGGSGGRVATSALEKQLQEILLKHLPAQSIASRALISTHGRPSIITRYWLPATALLLSSSTVLRILVNRQAELKQWLQELGATAVDFWTNWVVEPVRKIIGTIRHREDSEVALMSRSSLKADMESLERMVVDFAADNPATSLPEGSMPSPLTDLQIQAIRQAVHEGDLTPVLKSYEQELRAPFRGAVKGELLRALLIQIQKTKVDVEVAISGIDHLLKSQELVFGMVGLTPSALACVGIFQWARHSMGKGRGRRSGQRGGEMVRTLRNIDRILTGSAAYRTGERDDDRGGVLTYKEHGLLLCEVHVLREFAKKTLPIGVWAEFVEDVEELVDVRRGVKNQFRVLERIRWAYAKWIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GQ60</accession>
    <name>D5GQ60_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00012211001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic ribosomal protein eL14 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430382">
        <property type="protein sequence ID" value="CAZ86653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842462.1">
        <property type="nucleotide sequence ID" value="XM_002842416.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GQ60"/>
    <dbReference type="FunCoup" id="D5GQ60">
        <property type="interactions" value="1003"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GQ60"/>
    <dbReference type="GeneID" id="9185233"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012211001"/>
    <dbReference type="eggNOG" id="KOG3421">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082438_3_0_1"/>
    <dbReference type="InParanoid" id="D5GQ60"/>
    <dbReference type="OMA" id="KLCFVVD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030684">
        <property type="term" value="C:preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23702">
        <property type="entry name" value="eL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000030">
        <property type="entry name" value="60S ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039660">
        <property type="entry name" value="Ribosomal_eL14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002784">
        <property type="entry name" value="Ribosomal_eL14_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11127">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11127:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01929">
        <property type="entry name" value="Ribosomal_L14e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="KOW" evidence="7">
        <location>
            <begin position="15"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein eL14" evidence="8">
        <location>
            <begin position="50"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ86653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="135" mass="15461" checksum="19C0E9970632DF72" modified="2010-06-15" version="1">MSCDVKTTQWRLVEVGRVVLLSKGPYSGKLATIVEIIDHKRVLIDSPIVPRQSYSLKNVTLTPIVIDNLPRGARNGVVKKLWENCAVDTKWAESSWAKKITSKETRRNLSDFDRFRVLVLRKQRRFEVRKAAAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>D5GQ73</accession>
    <name>D5GQ73_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tyrosine--tRNA ligase</fullName>
            <ecNumber evidence="12">6.1.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Tyrosyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00012225001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>tRNA(Tyr) + L-tyrosine + ATP = L-tyrosyl-tRNA(Tyr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10220"/>
            <dbReference type="Rhea" id="RHEA-COMP:9706"/>
            <dbReference type="Rhea" id="RHEA-COMP:9707"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78536"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.1" evidence="12"/>
    <dbReference type="EMBL" id="FN430382">
        <property type="protein sequence ID" value="CAZ86666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842475.1">
        <property type="nucleotide sequence ID" value="XM_002842429.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GQ73"/>
    <dbReference type="FunCoup" id="D5GQ73">
        <property type="interactions" value="771"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GQ73"/>
    <dbReference type="GeneID" id="9185246"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012225001"/>
    <dbReference type="eggNOG" id="KOG2144">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035267_0_1_1"/>
    <dbReference type="InParanoid" id="D5GQ73"/>
    <dbReference type="OMA" id="RKIHMLA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990825">
        <property type="term" value="F:sequence-specific mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004831">
        <property type="term" value="F:tyrosine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006437">
        <property type="term" value="P:tyrosyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00805">
        <property type="entry name" value="TyrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.240.10:FF:000004">
        <property type="entry name" value="Tyrosine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000040">
        <property type="entry name" value="Tyrosine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.240.10">
        <property type="entry name" value="Tyrosyl-Transfer RNA Synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002307">
        <property type="entry name" value="Tyr-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023617">
        <property type="entry name" value="Tyr-tRNA-ligase_arc/euk-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050489">
        <property type="entry name" value="Tyr-tRNA_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006330">
        <property type="entry name" value="PRK08560.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00234">
        <property type="entry name" value="tyrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46264:SF4">
        <property type="entry name" value="TYROSINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46264">
        <property type="entry name" value="TYROSINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006588">
        <property type="entry name" value="TyrRS_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01040">
        <property type="entry name" value="TRNASYNTHTYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8 12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="374"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="376"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ86666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="418" mass="46139" checksum="0B3B0B1F3712DEBC" modified="2010-06-15" version="1">MASTTTTIDLTPHEKYDLITSNLQEVLKPNIIKSVLAENRPLKIYWGTAPTGRPHCGYFVPMVKLAHFLRAGCEVTVLLADIHAFLDNLKAPLELVTHRAKYYELVIKSVLRAIGVPIEKLRFVLGSSYQKGAAYVMDLFRLSSIVTEHDAKKAGAEVVKQVASPLLSGLIYPLMQALDEEHLGVDAQFGGVDQRKIFTLAAENLPGIGFKERAHLMNPMVPGLAGGKMSASDPNSKIDLLDGEEVVRNKLRKSHCAPSEVEGNGVISFVEYVLFPIAELADEKGEGKFVVPRDERWGGPVTYSSVVELKKDYQEDKLSPMDLKTGVEEALNKLLAPIRRDFEQDEEFRTVAKLAYPDEVKIKKKKEKKIGTGYVPKDKKTTAEKKVADGDAKPQETVEVAVGISAKDTQDKLAQNAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>D5GQ83</accession>
    <name>D5GQ83_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00012254001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430383">
        <property type="protein sequence ID" value="CAZ86676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842485.1">
        <property type="nucleotide sequence ID" value="XM_002842439.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GQ83"/>
    <dbReference type="STRING" id="656061.D5GQ83"/>
    <dbReference type="GeneID" id="9184033"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012254001"/>
    <dbReference type="eggNOG" id="ENOG502RHAD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006329_1_0_1"/>
    <dbReference type="InParanoid" id="D5GQ83"/>
    <dbReference type="OMA" id="WDNVECI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052761">
        <property type="entry name" value="Fungal_Detox/Toxin_TFs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47425">
        <property type="entry name" value="FARB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47425:SF2">
        <property type="entry name" value="FARB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="4">
        <location>
            <begin position="63"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="106"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="726"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="791"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="22"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="109"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="133"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="745"/>
            <end position="760"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ86676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="931" mass="103491" checksum="2FBA10EED1BC0CA1" modified="2010-06-15" version="1">MALAGTTATAAAPSSAISATTNLAPQPTTDSNSAVAGQKRSAAAAGNTSGTNFRPVKRRASKACQCCRARKVRCNVVEHGAPCTNCRLDEVECIITESRRRKKLWGTKVDNTGSASDTGADTTAQRQTNASPPERKTSPRRKNETHISGSPSAMPIDLPSDKESGGHVPHLIYQAQGLRMGCDRRASYASSHESLPSLANSLSTQTSSSSSHPRRLFTIPQACIELPAFVKPTPIKIPLDDLEYLTKKGALTLPEEPLRSELLRSHFDFVHPYMPVLNRKEFLRSVTSDDGSKGKISLLLFQAVMFAGSAFVDMESLRAAGYPTRKAARKAFFTKTRALYDFDHEQDRMSLVQAILLMTYWYETPDDQKDTWHWMGVAISLSHTIGLHRDPANSNMDPDRKKLWKRIWWSCFMRDRLVALGMRRPTRIKSEDCDVPMLTLDDFSIDTGPDGEEDSMFWSYGERQRKEMESRRQRELAILCIQKAKLCKVISRVLSAQYSVLNTNQGSLAADGSTRTTMMLLPKKLDPECCQVERCDEELARWVQELPEEAIYRPVGVNIPIDEVLTLHRNLLHMCYYTTVSALHRPQVLPSAPAPWPAQRNSSELQEVSRRKVRQAASEITHLAEELVDLDLVKYLPTTGVTVMLPAVIIHLLDIKSSNALTRELSLEGFGLCMQVMQGLRQSYSAADYATHFLEAAIKKAEIQVAGHKKLSQRRRARYHHHFDHLTPQKPTAIGSPHSTQFSGNAIAIPASAPPSNAALTPPPDIENTNMPDATPLDENELQLKLETFLQSPSSPKQDDHQAPTLHSLPQNSAATFTIGSNGVLRVREELLFPSERSAPMTPYLSVPRATDDEDRDLGALLNLDGQDNDPFFGHYDEAPLVGQKRFDGGTRAFDLVGNSSTWLEEDMSRILDGVDFDIDDVIQVLETEIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4F6</accession>
    <name>D5G4F6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00004087001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835278.1">
        <property type="nucleotide sequence ID" value="XM_002835232.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4F6"/>
    <dbReference type="STRING" id="656061.D5G4F6"/>
    <dbReference type="GeneID" id="9185024"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004087001"/>
    <dbReference type="eggNOG" id="ENOG502S997">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033337_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4F6"/>
    <dbReference type="OMA" id="MAMANNH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="208"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="164"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="261"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="429"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="180"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="261"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="326"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="338"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="378"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="448"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="474"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="508"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ79399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="520" mass="53980" checksum="278E78ED6C50C5BE" modified="2010-06-15" version="1">MAATKDSLSRYHPPSTPNRPPALHIKDVPVKTPPPSSSSSHHRSSLGSSGSSHRITRRKSMSSTSGANIAAVVQAAVRDGGEAPFALSVPASGIGMGLGRSPGSTKILNGSACGGGMEGYPSPPSSLPTTGPLGGGVGGIGGAVSGIAFTRKVGSLVSGGDSAIAEGHASDVGRPARSRRASEGAHVHLGESGDGVGGRVRARSGSELKCDKCGKGYKHSSCLTKHLWEHTPEWSYTSKLLISKHQQVQLLEAASILVSMNPTTTPPASSSGASNNEGNSSTNDSSSSSDDTTPPPSVFSGSDVTSPLPTPRGIRNGAGKPGKRYSSGSHSRSYQAPSGSGALAGSAPNSTAFPNHFPHHRPSRPRAGSSAGNIRGSHMTSPATRPTSITPEDEALAAAIELLSCSFNTPVMGPTVPLGSVPRGIAGLSSPPVSGRPGFLGPPLNDVVEMKREEGEEGDDVRMEDLEERRNVMMEEDDDEDEEEDDDDDEEEEEEEEEEVWKRRGRSEEDEDGVFGQMEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4M0</accession>
    <name>D5G4M0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="11">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00000005001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SecE/SEC61-gamma family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429989">
        <property type="protein sequence ID" value="CAZ79463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835306.1">
        <property type="nucleotide sequence ID" value="XM_002835260.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4M0"/>
    <dbReference type="FunCoup" id="D5G4M0">
        <property type="interactions" value="475"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G4M0"/>
    <dbReference type="GeneID" id="9188400"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000005001"/>
    <dbReference type="eggNOG" id="KOG3498">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_167752_2_0_1"/>
    <dbReference type="InParanoid" id="D5G4M0"/>
    <dbReference type="OMA" id="KPDQKEY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.820">
        <property type="entry name" value="Preprotein translocase SecE subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00422">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023391">
        <property type="entry name" value="Prot_translocase_SecE_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008158">
        <property type="entry name" value="Translocase_Sec61-g"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001901">
        <property type="entry name" value="Translocase_SecE/Sec61-g"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00327">
        <property type="entry name" value="secE_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12309">
        <property type="entry name" value="SEC61 GAMMA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00584">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103456">
        <property type="entry name" value="Preprotein translocase SecE subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01067">
        <property type="entry name" value="SECE_SEC61G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ79463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="70" mass="7729" checksum="3D4FBB8520E06301" modified="2010-06-15" version="1">MSDQFAQIADIPREFLKDGTLFINRCTKPDKREFIKISQAVGIGFVIMGVIGFIVKLIHIPVNNILVGAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4X1</accession>
    <name>D5G4X1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="11">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00000128001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429992">
        <property type="protein sequence ID" value="CAZ79564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835407.1">
        <property type="nucleotide sequence ID" value="XM_002835361.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4X1"/>
    <dbReference type="GeneID" id="9183046"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000128001"/>
    <dbReference type="eggNOG" id="KOG0768">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015166_3_0_1"/>
    <dbReference type="InParanoid" id="D5G4X1"/>
    <dbReference type="OMA" id="DIWIAGA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45667">
        <property type="entry name" value="S-ADENOSYLMETHIONINE MITOCHONDRIAL CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="90"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="185"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ79564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="277" mass="29809" checksum="D49F5E86A82B0E0D" modified="2010-06-15" version="1">MRTADLIAGPTAGLTVCLTFYPFDTLKTRIQSPAFRKLAAQSARPRKMWLRGLYQGIGPITLAMVPGSAVFFTSYESSKSFLRTSAPQIPEPITHVLSSSLAEALGCVVATPAEVIKQNAQVMHEAKGSSSLAALKLVGRNPKDLWRGFTLLAGRNLPFVVIQFPLYERLKVMFRVRERGNGLMETAKVTGAAAGLSGGVAAWLTTPVDMVKTIVMLGANKHSGRRLGAVDVAKSVWREEGVRGLWRGGALRGIWTGAGSGLYLGIYECTLLAMGEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4X3</accession>
    <name>D5G4X3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00000137001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429992">
        <property type="protein sequence ID" value="CAZ79566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835409.1">
        <property type="nucleotide sequence ID" value="XM_002835363.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4X3"/>
    <dbReference type="STRING" id="656061.D5G4X3"/>
    <dbReference type="GeneID" id="9183048"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000137001"/>
    <dbReference type="eggNOG" id="KOG0157">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_5_11_1"/>
    <dbReference type="InParanoid" id="D5G4X3"/>
    <dbReference type="OMA" id="WKHQRRT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11069">
        <property type="entry name" value="CYP_FUM15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002402">
        <property type="entry name" value="Cyt_P450_E_grp-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050121">
        <property type="entry name" value="Cytochrome_P450_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305:SF166">
        <property type="entry name" value="CYTOCHROME P450 12A4, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00464">
        <property type="entry name" value="EP450II"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0349">Heme</keyword>
    <keyword evidence="6 8" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="8">
        <location>
            <position position="497"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR602402-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ79566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="552" mass="59819" checksum="7415A40EC350DCDD" modified="2010-06-15" version="1">MSTTNILAGCAASTILLSRHTQFGAITTFILLTSLVLTCKLLYRVILWPKLLSPTRHLPGPSSPSFLMGNFAIIQKMQSGGPHAIWLRTIPNSGLLRYLGIFNTERVFPTTPEVLKEVLHTKSYTFIKPTLLTNDIGRILGKRGLLFAEGEEHKVQRKLLLPAFSHAHIKGLVPGFWSKGVEMTEKVAEVVRASQSAGGGEQGVVVEMGKWCSLVTLDIIGSSGFGYEFRALESASVSGSPEEKSGSELADAYNTIFNMGSPSRIVAILSLIFPAFLVQSLPLKRTRDVAHAASTIRRVTAQIIAAKKSALATSQEDAESKDILSVMLKSNAYTGSDGESSMRDQMMTFLAAGHETTATSMIWAIHTLSLPENRHIQSRLRAEIHAAFPSGAPATITYDQLSSLKYLSHITSEVLRLYPPVGVTLRVAAEDTSLNGVFVPKGTSIVISPFAMNRSAALWGADAEEFRPERWACGEDGTATVESNYGFLTFLAGPRGCIGNVFAKVEFKCLLAAAIGKFEFEQDGKRELVVKGSITAKPRGGMPVTVREVVWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4Y7</accession>
    <name>D5G4Y7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00000158001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429993">
        <property type="protein sequence ID" value="CAZ79580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835423.1">
        <property type="nucleotide sequence ID" value="XM_002835377.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4Y7"/>
    <dbReference type="STRING" id="656061.D5G4Y7"/>
    <dbReference type="GeneID" id="9183062"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000158001"/>
    <dbReference type="eggNOG" id="ENOG502RXKA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029630_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4Y7"/>
    <dbReference type="OMA" id="ECTYSFG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00657">
        <property type="entry name" value="Ferritin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052965">
        <property type="entry name" value="Pigment-catalase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31694">
        <property type="entry name" value="DESICCATION-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31694:SF26">
        <property type="entry name" value="OS05G0151100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13668">
        <property type="entry name" value="Ferritin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072211" evidence="1">
        <location>
            <begin position="19"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="305" mass="32054" checksum="7F88E0158F0A48BC" modified="2010-06-15" version="1">MLFPTTLALAASFMAANAAPLATREHINDGVILNYALTLEHLEDAFYTQGLKNFSSASSFSAFPPTLFPTLQTIASDEKTHVQFLQSALAAAGIPAVKPCTYSFPVTTPEEFVLVSGVLEGVGVTAYLGAAASIADKLTLTAAGSILTIEARHSSLIRAAQNQNPVPQPFDVPLDFNQVFSLASQFITSCPPENPQLPFKAFPTLSLAKNYTGGDGHVIKTGSKISLAPGVKTQRDGLSVAFLTVTGPIFVDAVFVDDHSDGFGHFEVVVPAGVNGQSYAVLVNGKEKVSDETIVAGPVIVEISN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5G550</accession>
    <name>D5G550_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00000255001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429996">
        <property type="protein sequence ID" value="CAZ79635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835478.1">
        <property type="nucleotide sequence ID" value="XM_002835432.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G550"/>
    <dbReference type="FunCoup" id="D5G550">
        <property type="interactions" value="222"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G550"/>
    <dbReference type="GeneID" id="9186333"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000255001"/>
    <dbReference type="eggNOG" id="KOG2369">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016065_2_0_1"/>
    <dbReference type="InParanoid" id="D5G550"/>
    <dbReference type="OMA" id="GGGSNWC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005637">
        <property type="term" value="C:nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097038">
        <property type="term" value="C:perinuclear endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046027">
        <property type="term" value="F:phospholipid:diacylglycerol acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006672">
        <property type="term" value="P:ceramide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140042">
        <property type="term" value="P:lipid droplet formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055091">
        <property type="term" value="P:phospholipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003386">
        <property type="entry name" value="LACT/PDAT_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11440">
        <property type="entry name" value="LECITHIN-CHOLESTEROL ACYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02450">
        <property type="entry name" value="LCAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072213" evidence="1">
        <location>
            <begin position="21"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="493" mass="54938" checksum="E3160B6BBD7152CE" modified="2010-06-15" version="1">MIPGVISTGLLSALTQLAESWGTGEKSRPYFRKRLWGSWSMLRAMVTDRATWKAHVMLDKETGLDPPGIKLRAAQGFDATDFFVTGYWLWSKIIENLASIGYDPTSAHTASYDWRLAYQNLELRDRYFSRLKNYIETAAKLSDKKVVLVAHSMGSQLAHYFFKWVEAEGYGDGGSRWVEDNIEAFINISGCMLGAVKGVPAVLSGEMKDTAQLNRFAVYGLEKFFSREDRAEIMRAMPGISSMLPKGGNAVWGNLTWAPDDSPSQNTSNGAFIKFTKGNYTEAHANLSMSESLDYLLENSDSWFHNMIRQSYSHGVALSGAEVEANQKIPQKWVNPLESRLPFAPSLKIYCFYGIGKPTERSYYFHENNDPLSRLNVSIDTTVSGEGSDRGVVTAEGDGTVSLLSAGYMCVKGWRLKRFNPAGVKIKTYEMPHQPEMFDIRGGPNTGDHVDILGRQKLNELILRVAAGDGDSISDNIESDIVAISERVKIYDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5K3</accession>
    <name>D5G5K3_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">E1 ubiquitin-activating enzyme</fullName>
            <ecNumber evidence="4">6.2.1.45</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00004382001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine = AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine.</text>
            <dbReference type="EC" id="6.2.1.45"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the ubiquitin-activating E1 family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.45" evidence="4"/>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835639.1">
        <property type="nucleotide sequence ID" value="XM_002835593.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5K3"/>
    <dbReference type="FunCoup" id="D5G5K3">
        <property type="interactions" value="1234"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G5K3"/>
    <dbReference type="GeneID" id="9183782"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004382001"/>
    <dbReference type="eggNOG" id="KOG2012">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002556_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5K3"/>
    <dbReference type="OMA" id="GANLHAF"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004839">
        <property type="term" value="F:ubiquitin activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01490">
        <property type="entry name" value="Ube1_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2660:FF:000001">
        <property type="entry name" value="Ubiquitin-activating enzyme E1 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12550:FF:000001">
        <property type="entry name" value="Ubiquitin-activating enzyme E1 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000015">
        <property type="entry name" value="Ubiquitin-activating enzyme E1 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.60:FF:000001">
        <property type="entry name" value="Ubiquitin-activating enzyme E1 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.80:FF:000001">
        <property type="entry name" value="ubiquitin-like modifier-activating enzyme 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.180">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, FCCH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.80">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, inactive adenylation domain, subdomain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12550">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, inactive adenylation domain, subdomain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2660">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, SCCH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.60">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, UFD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042302">
        <property type="entry name" value="E1_FCCH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018965">
        <property type="entry name" value="Ub-activating_enz_E1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042449">
        <property type="entry name" value="Ub-E1_IAD_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038252">
        <property type="entry name" value="UBA_E1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019572">
        <property type="entry name" value="UBA_E1_SCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042063">
        <property type="entry name" value="Ubi_acti_E1_SCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018075">
        <property type="entry name" value="UBQ-activ_enz_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033127">
        <property type="entry name" value="UBQ-activ_enz_E1_Cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000011">
        <property type="entry name" value="UBQ/SUMO-activ_enz_E1-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01408">
        <property type="entry name" value="Ube1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF4">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1 C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09358">
        <property type="entry name" value="E1_UFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10585">
        <property type="entry name" value="UBA_E1_SCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01849">
        <property type="entry name" value="UBIQUITINACT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00985">
        <property type="entry name" value="UBA_e1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00865">
        <property type="entry name" value="UBIQUITIN_ACTIVAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-activating enzyme E1 C-terminal" evidence="11">
        <location>
            <begin position="877"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="9">
        <location>
            <position position="585"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ79796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1011" mass="111671" checksum="6A148DA6A2209D4E" modified="2010-06-15" version="1">MTDTSSKMQLDSPADTATGTIDEGLYSRQLYVLGHEAMKRMSQSDVLIIGLKGLGVEIAKNICLAGVKSVTLYDPSRIEISDLSSQYFFQASDVGKQSDEVSAPLLGELNTYTPVSVLHSNPFDDVELLGRYKAVVVAGIPLSAQLKVNEYCHKNKIPYVSAETRGLFGSIFCDFGEGFIVIDPTGENPVGGIIAGIDSSGLVTALDETRHGLADGDHVTFGEVQGMESLNGAEFKVEVKGPYTFSIGEVKKPRIINFKSQAEQLKEPELLITDFAKFERPLQLHIGFQALHQFREKGGFPRPMNEEDAAEVLKYATSIADSLGESKPELDEKLIKELAYQARGDLAPMCAVIGGWAAQEVVKSLSGKFSPIVQHVYFDSLESLPTSVPRTEESVQPLNTRYDGQVAVFGKDFQDKIANVKEFLVGAGAIGCEMLKNWAMIGLATGPEGKISVTDMDSIEKSNLNRQFLFRSQDVGRLKSECAAAAVQKMNPDLNGKINTLRDRVGADTENVFDEGFWESLDGVTNALDNIEARTYVDRRCVFFRKPLLESGTLGTKGNTQVVMPGLTESYSSSHDPPEQSFPMCTVRSFPNKIEHTIAWSRELFEQYFVQPAENVNLYLSQPNFFEATLKQAGNQKQILETIRDYLVYNKPLTFEECIVWARHEFEKQYNNNIQQLLYNFPKDSTTASGALFWSGPKRAPDPLTFSLDNDTHMVFVKAAANLHAFNYGIKGNATDEVYRKVIGDMIISEFTPSSGVKIQASDAEPDPNATQTGFDDEGEIQRIIESLPPPSALAGYRLVKVDFEKDDDSNHHMDLITAASNLRALNYGIPTADKHTTKGIAGKIIPAIATTTSMVTGLVCLELYKVIDGKNKLEDYKNGFVNLALPFAAFSEPIASPKGKYQSKEGEVTIDKIWDRFYFDHDATLQEVLDVMASKGLTCSMVSCGVSLLYGSFFPQKKLKDRLPMKLTKLVQEISKKPVPAHTKNLILEICADDETGEDVEVPYICVRIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5R7</accession>
    <name>D5G5R7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00001450001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430001">
        <property type="protein sequence ID" value="CAZ79860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835703.1">
        <property type="nucleotide sequence ID" value="XM_002835657.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5R7"/>
    <dbReference type="STRING" id="656061.D5G5R7"/>
    <dbReference type="GeneID" id="9187538"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001450001"/>
    <dbReference type="eggNOG" id="ENOG502S7GF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_702467_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5R7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="274"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="78"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="157"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="175"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="238"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="274"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="288"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="308"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="331"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="393" mass="42757" checksum="39E6F7B9C5CAD4D4" modified="2010-06-15" version="1">MNTPPRLRSRGRPKTPPTPRHGPMFSSPEPQRTGKRKGTSSMSNYSTATLSPPPSSPIAPRSPDQTRTVKGKKRATFAIEGAAGASSSEIDSDGDKGGSAGREEQDDEIEKILGKNRKTLLNPGGFGLPTPAKTPSRKRKHLDLEEIAGSARVLFPSSSKSRASSSTSLSCARRTLFKKPSSRQRKGGMGSLDLLGDERPASDSISIFTDSNARIPKHDDDPDNPFISRPGEESVTSKRQRIKKKKKAGSLGADEDREDGMVYVFRGKKIFKKFTEMEDPNERLPNIEESTSSQPATSFTEALAAMGKQKELAEKRDISRLTPDSEDDSGHSSIFNPGNKTASKRRSIFDAAGDGDELVTAPVKKNLRRKRGLENGSATLESPRRARGKRARV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5G665</accession>
    <name>D5G665_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitochondrial distribution and morphology protein 12</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Mitochondrial inheritance component MDM12</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MDM12</name>
        <name evidence="12" type="ORF">GSTUM_00001629001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the ERMES/MDM complex, which serves as a molecular tether to connect the endoplasmic reticulum (ER) and mitochondria. Components of this complex are involved in the control of mitochondrial shape and protein biogenesis, and function in nonvesicular lipid trafficking between the ER and mitochondria. MDM12 is required for the interaction of the ER-resident membrane protein MMM1 and the outer mitochondrial membrane-resident beta-barrel protein MDM10. The MDM12-MMM1 subcomplex functions in the major beta-barrel assembly pathway that is responsible for biogenesis of all mitochondrial outer membrane beta-barrel proteins, and acts in a late step after the SAM complex. The MDM10-MDM12-MMM1 subcomplex further acts in the TOM40-specific pathway after the action of the MDM12-MMM1 complex. Essential for establishing and maintaining the structure of mitochondria and maintenance of mtDNA nucleoids.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the ER-mitochondria encounter structure (ERMES) or MDM complex, composed of MMM1, MDM10, MDM12 and MDM34. A MMM1 homodimer associates with one molecule of MDM12 on each side in a pairwise head-to-tail manner, and the SMP-LTD domains of MMM1 and MDM12 generate a continuous hydrophobic tunnel for phospholipid trafficking.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Mitochondrion outer membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
            <orientation evidence="9">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Endoplasmic reticulum membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
            <orientation evidence="9">Cytoplasmic side</orientation>
        </subcellularLocation>
        <text evidence="9">The ERMES/MDM complex localizes to a few discrete foci (around 10 per single cell), that represent mitochondria-endoplasmic reticulum junctions. These foci are often found next to mtDNA nucleoids.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the MDM12 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430008">
        <property type="protein sequence ID" value="CAZ80008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835851.1">
        <property type="nucleotide sequence ID" value="XM_002835805.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G665"/>
    <dbReference type="STRING" id="656061.D5G665"/>
    <dbReference type="GeneID" id="9187425"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001629001"/>
    <dbReference type="eggNOG" id="ENOG502S3PB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026794_0_0_1"/>
    <dbReference type="InParanoid" id="D5G665"/>
    <dbReference type="OMA" id="KRAHFCF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032865">
        <property type="term" value="C:ERMES complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990456">
        <property type="term" value="P:mitochondrion-endoplasmic reticulum membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015914">
        <property type="term" value="P:phospholipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045040">
        <property type="term" value="P:protein insertion into mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd21672">
        <property type="entry name" value="SMP_Mdm12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03104">
        <property type="entry name" value="Mdm12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027532">
        <property type="entry name" value="Mdm12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28204">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28204:SF1">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26544">
        <property type="entry name" value="Mdm12"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="6" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 9" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SMP-LTD" evidence="11">
        <location>
            <begin position="1"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="70"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="192"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="70"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="98"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="123"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="192"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="213"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="233"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ80008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="417" mass="45991" checksum="A10C392D3DDDF07D" modified="2010-06-15" version="1">MSIEVNWETLTAGPDGARYAENIRSFVHEKFQSIPFPKFIKSVNVHSFSFGNVPPDVEIKELCDPYPEFYEDDDDECSDSDSADIDNGNVGGEDEQDYLLKLERERRRRERRDDGYTIGGARSPPPGLNPPPYTPDGRTGIPFRSPFTPGIPDTFPGRVGVAGVTTPLFHRGGSSSNLHYFHSALSSGFTGTNTPTLPAFQSPTSPGPTENQPRSSSTSSLPSRPSTSDSGGPISSLNITDPPSVGSSAQEPLHTPTSEDSRARGSSSSAIPRLTEPRDQDTQIMARVRYSGDIRLELTAELLLEYPITNFVALPVRLLVTGCKFDGLACLAYIRRRAHFCFIDEEQTPDLGDGEGVTRVRTKSLLKEIKVESEIGEKGKGKQVLKNVGKVERFVLEQVRRIFEEEFLFPSSWTFLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5G669</accession>
    <name>D5G669_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00001635001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Znf_NHR/GATA"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PAS" evidence="6">
        <location>
            <begin position="129"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="GATA-type" evidence="7">
        <location>
            <begin position="417"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="283"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="369"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="299"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ80012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="453" mass="49614" checksum="A6DD172516F80B70" modified="2010-06-15" version="1">MMNHPSSSSPAQSYNVPASMRSAHMYAGSMPLVNGVNGQLQNMQISPLAAQQAMSAGGLMGDPNNNPLLDPSVIMSMGMAGEDGMPLDPQLCTGGDQFGPLSVTAPPTSMMTEYGNLGGGSTLTEFTKRRNWSQRILEELQDFLHILSPSGKMVYASPSSKALTGFGPEELLGKFITEFIHPDDSGLFVREFNGAIATGQPLRLFYRFQKSDGTWAIFETNGHPHIGEAPQFSPSSIQSVCKGFFMMSRPYPTRNASLLDSFLEHKIENERLLKRISELRKEEFEDHQQQQRQRQQHHATVSRTDSGTAGSSMASNVGNDNPMATTPNSSDPMPPPAKPTHIRDKMARFEGATHMDTIEMLTGLRYREGERSKGISTGDTSPALIRGDAGVPIPIDKESRHTSEKKKKQKIADEYVCTDCGTLDSPEWRKGPKGPKTLCNACGLRWAKFVTPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6A3</accession>
    <name>D5G6A3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00001675001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430008">
        <property type="protein sequence ID" value="CAZ80046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835889.1">
        <property type="nucleotide sequence ID" value="XM_002835843.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6A3"/>
    <dbReference type="STRING" id="656061.D5G6A3"/>
    <dbReference type="GeneID" id="9187717"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001675001"/>
    <dbReference type="eggNOG" id="ENOG502R2RI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005801_0_0_1"/>
    <dbReference type="InParanoid" id="D5G6A3"/>
    <dbReference type="OMA" id="LQHENEN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="38"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="125"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="276"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="457"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="623"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="673"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="830"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="218"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="171"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="198"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="457"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="642"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="739"/>
            <end position="751"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ80046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="902" mass="98804" checksum="CE9E9B239E8504EA" modified="2010-06-15" version="1">MAFVALATLIALIWMCYRASSLFKIPIPALVKLLGLDLPDPPRVSLHGIAADTITLHWSLPEKAGSVAKHIIQINGINVGESEKRGETSVTVTGLNADHLYNVRVIAANSQNFQAPGQLIRLRTRRVSQASNGGAPLATGRDSSDDLPAIHALPPALEPQPPHHHHHHHSASGSGNSNGNNNNSTSNHRRAAKERKSSPASLGQQGQTEPPAEEQHTVESLTASLEIVRQEIAETDAQLAHTEEEFKAAEAVLRGELDLLKERKNEEDLGRQKLRAETRALEEGKRASEATRSKTERALKSKEDEIKKMRNDSLRWDEERVAALQRVKELSKAAEESRKHAQITERELQEEMKETQKAILEMEEEIRTRAGSIKVLEAQRELWKAEEEVESKKMAEDEQKEMVWKERQRHLEVRYITVYNAYQVAEFDYMRSRDAFLSSQSRRGSAGTDPIVLPAKKAKQRRNRNRKSRTQTISSPTGAVAPQDARFPEVPAGGMSIGQMNTPFSGGVNPSPFFNIANGTVGAPTEPPGPPLKASFFVDLDRMGGRAPMSPTANSLLPSNLFAFDDSLPSPKGSASLLEDGQSRYSGIDPLGFMAPQSPMSSGSVSTHVSSPRSSFHHIPMFPNMSSDGNPGDAERGNSIPLLDSGLGQLGSSEEENISGGRRFVKLFSRQRGKTIPLDGGPPLGSLKPNESHSFPKGPHPPGLDPIGTRRRSGSYGSWTTGLEFLHGGRKKPLERNDSSSSRRSGFNHFSPSFDPIEPGKLLDTTVSPRPSSIASFDNLLPAPSSDVSAAFGWPAAPPAIEQPRNRTEPGSAHFPTDLTQCKHINKQSLLLQPPLPIRTPGNSKTEPRGANLRSPRRAKGGECILRYIHQYGYVQYFKRIRDAEGLTAVQILGAKQEGQHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6F5</accession>
    <name>D5G6F5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="12">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00004461001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430009">
        <property type="protein sequence ID" value="CAZ80098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835941.1">
        <property type="nucleotide sequence ID" value="XM_002835895.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6F5"/>
    <dbReference type="STRING" id="656061.D5G6F5"/>
    <dbReference type="GeneID" id="9181183"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004461001"/>
    <dbReference type="eggNOG" id="KOG0800">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008264_3_1_1"/>
    <dbReference type="InParanoid" id="D5G6F5"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16454">
        <property type="entry name" value="RING-H2_PA-TM-RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46539">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE ATL42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46539:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE ATL42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="225"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="383"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="192"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="432"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="512"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ80098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="566" mass="60321" checksum="B8B77D13C56D6ACD" modified="2010-06-15" version="1">MFGSTRCAINRGGFCSIPFANLLVSLIAISGFSLADQIVSAQDNDNLLSRSGLVLGTTDEVRPISVPITQWLGSSGENSLKDTVRTATLAGNLNLYDSSSNTELTVADFAYIDCDKENATQAYQNLVSNRGVQGGSIPYAAIFFTTKHDYCKWQGTENFTSPYILTTLERRRAAAFQNLLLTTKTPVTVSVSYNESDDGAMPDSGTNEHRNDPQNPLGPAPSTGVALIILYSITGVITALFLLIIVTGAIRAHRHPDRYGPRSTARFGRPRQSRAKGLARAVLDTLPIVRFGGAKEGGVKSDVELQEPAAHASDVDGLERSTTQVLPSQAIPAAGTLAAVPRSDAPASAATATTGEEEATGEVGVGIGAGESQAELDVDQVRCPVCQEDFEQGQDLRVLPCRHSFHPDCIDPWLLNVAGSCPLCRIDLRPDDQRQSFETPPPEPDSPPHNRHSQGSRSTRHIDNARQGQTGEERVEALRQLREESGANGGRHRGLTPELGLSRLRHFLGRRMVSNRGSTMEGDALARGGDSPPGVPASATIPPEGGAAAAGDTSNPSDVGVSERRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6J3</accession>
    <name>D5G6J3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00004507001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RICTOR family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430009">
        <property type="protein sequence ID" value="CAZ80136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835979.1">
        <property type="nucleotide sequence ID" value="XM_002835933.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5G6J3">
        <property type="interactions" value="447"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G6J3"/>
    <dbReference type="GeneID" id="9181221"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004507001"/>
    <dbReference type="eggNOG" id="KOG3694">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001013_1_1_1"/>
    <dbReference type="InParanoid" id="D5G6J3"/>
    <dbReference type="OMA" id="EIRIHAT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031932">
        <property type="term" value="C:TORC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038203">
        <property type="term" value="P:TORC2 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.160">
        <property type="entry name" value="HR1 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036274">
        <property type="entry name" value="HR1_rpt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028268">
        <property type="entry name" value="Pianissimo_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028267">
        <property type="entry name" value="Pianissimo_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029453">
        <property type="entry name" value="Rictor_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029451">
        <property type="entry name" value="RICTOR_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029452">
        <property type="entry name" value="RICTOR_V"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13298">
        <property type="entry name" value="CYTOSOLIC REGULATOR PIANISSIMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13298:SF11">
        <property type="entry name" value="RAPAMYCIN-INSENSITIVE COMPANION OF MTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14663">
        <property type="entry name" value="RasGEF_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14666">
        <property type="entry name" value="RICTOR_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14664">
        <property type="entry name" value="RICTOR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14668">
        <property type="entry name" value="RICTOR_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01303">
        <property type="entry name" value="RasGEF_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01307">
        <property type="entry name" value="RICTOR_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01308">
        <property type="entry name" value="RICTOR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01310">
        <property type="entry name" value="RICTOR_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46585">
        <property type="entry name" value="HR1 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rapamycin-insensitive companion of mTOR N-terminal" evidence="5">
        <location>
            <begin position="229"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="domain" description="Rapamycin-insensitive companion of mTOR middle" evidence="4">
        <location>
            <begin position="671"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="domain" description="Rapamycin-insensitive companion of mTOR" evidence="6">
        <location>
            <begin position="1082"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="102"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="13"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ80136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1301" mass="145230" checksum="9D2EE05F00F9D1EE" modified="2010-06-15" version="1">MSNALTTSPKSVSGASSPGPRARGASGGDRDGLLHLRQPNGNRAGSSFGGGSGTTIVNSTLNGPGSFSASLRSTNTIVAMTPNAFVEEMSNPPSPMSSERLVEELTDQLNKAIKYKEGAENLLQVLDTKKPKEAKQARNQAEKEYNARNQEISQLMNRIEAITKPREVTGATRAANKFKGEPIRPTAANNGLAISGAPEWDPGSESPTLSLTELLKDLEEVGRRPEYYVEKANSLVALFKRHPNLKYELAWNNFGSRVQNMLLNENKEVVAAGYRIARYAMTDLQSLKTIRALYTDEITIISLVKESRANVEREQALKFVRAFIEIPGGVQEISRGVVRAIVACAEQVDDRLRGVSMETLAEILILNPSLVVSAGGVRVLTQVLSEGPYELSDTISLAFLYLLDLPATRKYIRPGNDLEIVFSVFTDSYSEKSPAPEERLRSSAKVISCMLKSWPGLLVLSMYDLRTPKSLVSALSIPSSSVRDIILELFFDIFRIKPPSWSSSFLAGRRLTTYGRVTNLKSESDAPKPPRADDAYQGNLVEHFTTLLLAVFFEADLLRSLMALVEDNSDPAIVRKTTLLLGEILKLASRLLPSTYSARLQLLPELFISAANFRVPTRFAASAAVYQIDSLNRTLHRSLAATQAPQRVSVDDEKRGQRQVEQAKLKIYMAIDDRHFSGLIVDSGVLNAKSHTKWNWDALNEMIQGPLLNPKRLDEAIKGTKFMKRLMSFYRPFKYKFSDVKNTKPNQKYVRTGCALFHTLLQTPEGVRYLSENKILRQIAECLAQLDRMSGITSPEPLFSTSRLIGTLSCGYFAMLGVLSSDPKGLAMMERWRMFNMLYHISDSLDRPDLLELFVSNMDFTLEGHPRIILSKALTMNLKNVRLFATSHLRSLISTAQSSQTPIDSKTNETAEWAIRLLITQLYDPDVEVCETAVKILEEACNITHSLEYVVKCRPALDHLGEIGAPLLLRFLSTSVGYHYLNELDYINREMDDWFHGRNDSYVLTVEASLARAFADEEPLKKRSGDINTIDRDMMGIVPPHFYRELARTSEGCILLREKGHFNDFAWYIREHGQEEEDAEIVTKVKGCLWAVGNIGSMPFGAPFLEEADIVESIVKIAEESQVLTLKGTAFFVLGLISRTMQGLEILLEYGWDGTTSTMGESLGFCIPLKLGRLLSVSSVAPWYHTGDETNQINRPSPLSSVIGDDPVDAKILTAIANLNNHILESGSTKELNRLKASHVMQFQSPDLFRRVMTLIESFRYRQHVRRYILDLFEKSVVERVFAEGRADPEVESPDTPEGFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5G724</accession>
    <name>D5G724_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00004569001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430020">
        <property type="protein sequence ID" value="CAZ80317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002836080.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G724"/>
    <dbReference type="FunCoup" id="D5G724">
        <property type="interactions" value="250"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G724"/>
    <dbReference type="GeneID" id="9182508"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004569001"/>
    <dbReference type="eggNOG" id="ENOG502QQTI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007201_1_0_1"/>
    <dbReference type="InParanoid" id="D5G724"/>
    <dbReference type="OMA" id="DWHPRNL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009074">
        <property type="term" value="P:aromatic amino acid family catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.240.10:FF:000012">
        <property type="entry name" value="C6 transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052780">
        <property type="entry name" value="AAA_Catabolism_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31644">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR ARO80-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31644:SF2">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR ARO80-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="3">
        <location>
            <begin position="48"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="127"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="203"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="815"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="158"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="215"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="256"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="815"/>
            <end position="826"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ80317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="920" mass="100617" checksum="047028F39CDF9EA0" modified="2010-06-15" version="1">MSYDPRTTDEAPPNATFSTVPPSATCATGIPANPTPPPPTIHKRTYQACIPCRQRKVRCDLGSVEAPHDPPCVRCRRESKECYFSATRRKTRTPSTDEVGIGRGRGTIRGVVRKHEDDEEVLSPIKRSRSIGHGDFANSPSYSPSFAGKPFVTPIDPRLAERDGDVDSRDGDTATAAEALLQTPVCNSHQALLALVEVAGRDAPMSTGGKSPGDTDGEGEKDVDDSLSPATRNGVGVRFSSAGDGTFSPYARRRAGSNSTTSPGTQAGRPGTARSLSISTIHEDKDGLEKAAGWFTAREAIQYVEYFYKHLHPLSPTLTSPSLQHISHPSHHPSLLRLEPFLTTIILLITSRYMVLSGPGGQSRSYAIHDMLWREVRGGCEGLMWGGGWGGLGTASSNNHSYTHSAKVARGLRTLGTVEGLLILTEWHVRGLHFPMDVEEGDWMVDSSDSEECETTHTRRKARKGGGVVEGLSDRIENILEPAYRSDRMSWMLLGNALALAYELGVFDEIDEPASTTPAHLPSREPPPPDTGYRLRFQRIQKLLLLYVLQLASRLGWTSMIPRHITQSVQHKLNSAGASPASVTITGRWDSEDELQDVIFHSWVDLTMVMKHSAETLFPSRRHTKEIMRTGRYVELLEYFQPKLKDWKRDFDALNLPNPIHHILSMEYEHVRFYINSLALQAVVDRCTVNNSTKTAMMQVNAKDVSFIKEVVDASRALLSCVVKKMCPGGYLKHAPVRIYLRILGASMFLLKTLALGAKESDVTTSIDLIHDTIRALRSASVDDVHLALRFADLLEILTNRVNARFIRINPTSSATTNATADTSANTPPPQATSPKTKVRMLPPPTPGTLQADMARGDYGYDGADIHDAEFLFSGYEWLALPIDPVTGAGFDAGVTQSQMGVDVGGVDLLELLLAGSGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>D5G729</accession>
    <name>D5G729_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00004589001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430020">
        <property type="protein sequence ID" value="CAZ80322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836131.1">
        <property type="nucleotide sequence ID" value="XM_002836085.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G729"/>
    <dbReference type="GeneID" id="9182513"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004589001"/>
    <dbReference type="HOGENOM" id="CLU_734017_0_0_1"/>
    <dbReference type="InParanoid" id="D5G729"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="232"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="300"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="365"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="377" mass="40414" checksum="A6948B49390883F7" modified="2010-06-15" version="1">MRLLPLYPFLSQFFVPLSSAPAPLFSTLRWQSFVCFLFSTLPILAPPFFLGDSTIDRVRSASRYSFSCVTDQNHPALLRQFSLSHSDNTMPRKIREKVPVMRACDLCAAHRRRCEHKVAAYLPKGGGGRDAKPRPVEPADEAMPDAASDSGAKASKRKAAQMEGDAPEVVRSVRRRSLRSGGGDFVRKVDQQDSDDGKPVSKGLSVGSAEGEEPPEAPNGLQHGEGGEGYEDSSSVSGGDPHGESSPSRSVSPPVIEIASPTFSIAPEVETRGPSPFSDDSEEEGVCVRLPPAPSPPPTRSQLPSSNASPVPESSPSTPSTPSPQQAPIRPYSRWVDPTKPASRFSGGRRYTPLTLNTPIYGGRIPPPAPTPPPTSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5G779</accession>
    <name>D5G779_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00002504001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430022">
        <property type="protein sequence ID" value="CAZ80372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836181.1">
        <property type="nucleotide sequence ID" value="XM_002836135.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G779"/>
    <dbReference type="GeneID" id="9185270"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002504001"/>
    <dbReference type="HOGENOM" id="CLU_2051367_0_0_1"/>
    <dbReference type="InParanoid" id="D5G779"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003071998" evidence="1">
        <location>
            <begin position="18"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="120" mass="13159" checksum="932ADE8D99EA0F18" modified="2010-06-15" version="1">MLVLLFFYASACTVVDGASPSHEVSLEPPAPRDAVVGRQDTVAGERHRAGISEAYSLILPVTHPPRERMRGCMGTQILEKGRHSHYRGGQPKASEGRTLASSIVPYCTRQTWVLLELPMC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7C3</accession>
    <name>D5G7C3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00002437001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430023">
        <property type="protein sequence ID" value="CAZ80416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836225.1">
        <property type="nucleotide sequence ID" value="XM_002836179.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7C3"/>
    <dbReference type="GeneID" id="9185314"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002437001"/>
    <dbReference type="eggNOG" id="KOG4569">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032957_1_0_1"/>
    <dbReference type="InParanoid" id="D5G7C3"/>
    <dbReference type="OMA" id="CTRCECT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00519">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051299">
        <property type="entry name" value="AB_hydrolase_lip/est"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002921">
        <property type="entry name" value="Fungal_lipase-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46640:SF1">
        <property type="entry name" value="FUNGAL LIPASE-LIKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46640">
        <property type="entry name" value="TRIACYLGLYCEROL LIPASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G06510)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01764">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072625" evidence="4">
        <location>
            <begin position="25"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="Fungal lipase-type" evidence="5">
        <location>
            <begin position="162"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="57"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ80416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="357" mass="37823" checksum="EE911C23AFCC8738" modified="2010-06-15" version="1">MRGATTLLAIISGLSGSYIQASSAAPTPVATSGAGDLFENIWENLVETGKDIIGGDNPSTSKGSKSLSTPTSINIKPTIEPNTNQCKALDPKLLPKILYFAEFAAAAYCDPNHVVGTEVTCAGGVCPEITTNNITTILEFANTQDADTTGLVARDDTVKSIVISIRGSSSLRNWLANIQAKLKKVPEICPGCEVHSGFYEAMQEALPAVVKSVEELKRENPGYTVVVVGHSLGGAIATLMAEEIRRGGVEVDLYTFGAPRIGNEELSTFISKSGTNFRVTHTVPRLPPVILGYQHISPEYWISKGDTNISATDIQIFEGGINTGGNSNGPISLNIPAHSHYLLPVEISSCAPSGFEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7E8</accession>
    <name>D5G7E8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00002532001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430026">
        <property type="protein sequence ID" value="CAZ80441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836250.1">
        <property type="nucleotide sequence ID" value="XM_002836204.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7E8"/>
    <dbReference type="STRING" id="656061.D5G7E8"/>
    <dbReference type="GeneID" id="9188466"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002532001"/>
    <dbReference type="eggNOG" id="ENOG502QRXF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010063_2_2_1"/>
    <dbReference type="InParanoid" id="D5G7E8"/>
    <dbReference type="OMA" id="RTFTACI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010816">
        <property type="entry name" value="Het-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052577">
        <property type="entry name" value="VWA7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14905">
        <property type="entry name" value="NG37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14905:SF7">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07217">
        <property type="entry name" value="Het-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072232" evidence="2">
        <location>
            <begin position="28"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="467"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ80441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="492" mass="53989" checksum="93459988FB825E6D" modified="2010-06-15" version="1">MAMCKRTSLLLLFTILLFLTLASPAAAFGAGNIASIAKIEGRNWRHGDVEDTLKTLLALRGGKWTAMNVRRVYFGNWLRDYSQALDVGTLKSVNKDTIRILVWVLSFLSFGYATGEFEVTEERLGVYRPEEHIDNPKDYADNMDARHYDPRLRPPVHPAELAIDPLTGMKNYIANETGGWATSTGYVKHSLTRSIHFGRLYTSGGTGIAGREGDLCEALRCLGQALHCLEDFSAHSNYVELVLRELGFHSVFTHTGRVFPLVTGTFGAVDFLHSVLGEAQDKFSQTEVDELDNVLGEAHEQQKKSERSGQKSVLQGLLQQIPNLGGLSGDADRLEAQSNAKEMENMHISPQQSRGPAGAGAVPLSGGFDPAKTARDIYPILEFRDKVMRCIEETIEQIPGLSALVEKISETLSLFILSLLAPFVRPIIAQASLELKMGSSAVVDSSAAHQYEPWTVPTCTDPTHSLLSKDHFSNKLNTPAGESTPGRTPPPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7I5</accession>
    <name>D5G7I5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA repair protein RAD51 homolog 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00002577001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430028">
        <property type="protein sequence ID" value="CAZ80478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836287.1">
        <property type="nucleotide sequence ID" value="XM_002836241.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7I5"/>
    <dbReference type="STRING" id="656061.D5G7I5"/>
    <dbReference type="GeneID" id="9186961"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002577001"/>
    <dbReference type="eggNOG" id="KOG1433">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043547_2_0_1"/>
    <dbReference type="InParanoid" id="D5G7I5"/>
    <dbReference type="OMA" id="TQMTSKV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033063">
        <property type="term" value="C:Rad51B-Rad51C-Rad51D-XRCC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033065">
        <property type="term" value="C:Rad51C-XRCC3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005657">
        <property type="term" value="C:replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008821">
        <property type="term" value="F:crossover junction DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000400">
        <property type="term" value="F:four-way junction DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000707">
        <property type="term" value="P:meiotic DNA recombinase assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01393">
        <property type="entry name" value="RecA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052093">
        <property type="entry name" value="HR_Repair_Mediator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013632">
        <property type="entry name" value="Rad51_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46239:SF1">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD51 HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46239">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD51 HOMOLOG 3 RAD51C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08423">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecA family profile 1" evidence="9">
        <location>
            <begin position="29"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="318"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="17"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="331"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ80478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="367" mass="38227" checksum="933EA74CFB659B2A" modified="2010-06-15" version="1">MASTSPLPSSSHRLPTLSASQALSTSQSTRSRISTGSQELDKAIGGGLSRGKITELCGPPGSGKTTLGLQSAANALLSGDGTRVVWIDTSHPLPVSRLGAILSRSGDPRELFPNLQTIRTPTLAHLLTLVVHPLPTFPPENTSLVVIDNVSTPFTVAFPPGMEEEGGGGGGKVKTEPVGVRRFAIMGDLISALGKLAGSKGVAVLLLNQMTTKVIPGVGAMLIPSLSSPPWTTSLNSRLLIHYNKTSPSLLADPDDTRLSQIRYVTILKSNGASHSSDSSRPVTRLQIQETGVGDVKLVSEASPAAPAMKAYGFARGKRKRGLEAEVIPGSDEEGDGESDGNEDEGSAGECTSGRRQQRVIDSAQGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7Q9</accession>
    <name>D5G7Q9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00004684001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430031">
        <property type="protein sequence ID" value="CAZ80552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836361.1">
        <property type="nucleotide sequence ID" value="XM_002836315.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7Q9"/>
    <dbReference type="GeneID" id="9187031"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004684001"/>
    <dbReference type="HOGENOM" id="CLU_2980781_0_0_1"/>
    <dbReference type="InParanoid" id="D5G7Q9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="58" mass="6486" checksum="1D5022D55FE8E647" modified="2010-06-15" version="1">MQSSVKKNPTIAKPLNFPIRQGITKTHSRTPQAERNYPADQCGSNRHNLISTSLQQPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7V8</accession>
    <name>D5G7V8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Aspartate--tRNA ligase, cytoplasmic</fullName>
            <ecNumber evidence="3">6.1.1.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Aspartyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">GSTUM_00002673001</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>tRNA(Asp) + L-aspartate + ATP = L-aspartyl-tRNA(Asp) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19649"/>
            <dbReference type="Rhea" id="RHEA-COMP:9660"/>
            <dbReference type="Rhea" id="RHEA-COMP:9678"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.12" evidence="3"/>
    <dbReference type="EMBL" id="FN430032">
        <property type="protein sequence ID" value="CAZ80601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836410.1">
        <property type="nucleotide sequence ID" value="XM_002836364.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7V8"/>
    <dbReference type="FunCoup" id="D5G7V8">
        <property type="interactions" value="1167"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G7V8"/>
    <dbReference type="GeneID" id="9181420"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002673001"/>
    <dbReference type="eggNOG" id="KOG0556">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004553_2_1_1"/>
    <dbReference type="InParanoid" id="D5G7V8"/>
    <dbReference type="OMA" id="WVHEIRD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004815">
        <property type="term" value="F:aspartate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990825">
        <property type="term" value="F:sequence-specific mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006422">
        <property type="term" value="P:aspartyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04320">
        <property type="entry name" value="AspRS_cyto_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00776">
        <property type="entry name" value="AsxRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000013">
        <property type="entry name" value="Aspartate--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000132">
        <property type="entry name" value="Aspartyl-tRNA synthetase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02075">
        <property type="entry name" value="Asp_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004523">
        <property type="entry name" value="Asp-tRNA_synthase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00458">
        <property type="entry name" value="aspS_nondisc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003483">
        <property type="entry name" value="PRK05159.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43450:SF1">
        <property type="entry name" value="ASPARTATE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43450">
        <property type="entry name" value="ASPARTYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="15">
        <location>
            <begin position="243"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="31"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAZ80601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="552" mass="61458" checksum="BA8B054DA3A1F5FD" modified="2010-06-15" version="1">MSSTEPPTDQILAEGEPSVNVPIGPDGKPLSKSAIKKLQKEREKAEKAAKRAALEAEQKAAREAANLDHATGNYGTKKMIQSTERSGTKRIQLSKLTAEDDGTEVVFRARLQNARAQGAKMCFVVLRQQSDSIQAVLTVNTEGTVSKQMVKWASGVNLESVVLVHGKIAKTPEPVTSASLSDVEVQITKFFVLAEAEPMLPIQFDDASRSLKEVEAGAATVSLATRLDNRVIDLRILSNQAIFNISSAVCTLFKEYLLKHNFVEVHTPKLIAAASEGGSNVFKVHYFNRDAYLAQSPQLYKQMLIAADFERVFEIAPVFRAENSQTHRHMTEFTGLDLEMAFEEHYHEVVEVLEGLFVYIFAGLQERFAKEIAIVRAQYPVEEFKVPKDGKVLRLTFKQGIAMLREAGHEVPDLEDLSTEMERTLGRLVAEKYGEDFYILDKFPLAVRPFYTMPDPEDPQYSNSYDFFMRGEEILSGAQRVHNSQFLAERMSSLGIDPEAPGLEDYVGAFKYGAPPHAGGGIGLERVVFLWLGLGNIRRASAFPRDPVRLRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5G832</accession>
    <name>D5G832_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00002788001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430039">
        <property type="protein sequence ID" value="CAZ80675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836484.1">
        <property type="nucleotide sequence ID" value="XM_002836438.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G832"/>
    <dbReference type="STRING" id="656061.D5G832"/>
    <dbReference type="GeneID" id="9184148"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002788001"/>
    <dbReference type="eggNOG" id="KOG0894">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041481_3_0_1"/>
    <dbReference type="InParanoid" id="D5G832"/>
    <dbReference type="OMA" id="DEENWKW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0180027">
        <property type="term" value="P:inner nuclear membrane-associated protein degradation pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd23799">
        <property type="entry name" value="UBCc_UBE2J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000058">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="275"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="UBC core" evidence="4">
        <location>
            <begin position="5"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="218"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="218"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ80675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="293" mass="31571" checksum="36292CEFAC6862D2" modified="2010-06-15" version="1">MATRAANKRLVREYKAITENPPPYIQAHPSESNILEWHYIITGPEKTPYENGQYWGTLVFPADYPFKPPAIRMMTPSGRFQTSTRLCLSISDFHPKSFNPAWQVSTILMGLLSFMTGDEMTTGSISATDQERRILAARSRWWNSTGGGSGVLDPVSGIVVPNRAPGVGIGTGGSRGGVGNGDLGRKFRGEWKDVDAENWRWLKAKNFNLASGLISAPPSASARPPAGSGVAGAAGTDTEGARRRQRSGSIGNTGTAGAAGVVVRRGGGWFERHRVLVGIVFLFVWALLARSLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8B3</accession>
    <name>D5G8B3_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA exonuclease 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00002961001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Exoribonuclease involved in ribosome biosynthesis. Involved in the processing of ITS1, the internal transcribed spacer localized between the 18S and 5.8S rRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the REXO4 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430041">
        <property type="protein sequence ID" value="CAZ80756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836565.1">
        <property type="nucleotide sequence ID" value="XM_002836519.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8B3"/>
    <dbReference type="FunCoup" id="D5G8B3">
        <property type="interactions" value="754"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G8B3"/>
    <dbReference type="GeneID" id="9188358"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002961001"/>
    <dbReference type="eggNOG" id="KOG2249">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022453_2_1_1"/>
    <dbReference type="InParanoid" id="D5G8B3"/>
    <dbReference type="OMA" id="SQKEMRF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06144">
        <property type="entry name" value="REX4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000007">
        <property type="entry name" value="Interferon-stimulated exonuclease gene 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037431">
        <property type="entry name" value="REX4_DEDDh_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047021">
        <property type="entry name" value="REXO1/3/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801:SF45">
        <property type="entry name" value="RNA EXONUCLEASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801">
        <property type="entry name" value="RNA EXONUCLEASE REXO1 / RECO3 FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Exonuclease" evidence="11">
        <location>
            <begin position="149"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="122"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="312"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="19"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="35"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ80756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="338" mass="36737" checksum="3460D6DDC824AAAF" modified="2010-06-15" version="1">MATTALSRNWKQLQETLKKSSSTTPTNSSKRKSSSIESPTSSSSSTITRAKKAKRARTPSSTAANPANPPLIPRASKPTPTSKVLKMSTAAPKPTSSALALWAEDNDISPADLQKAYNLPLTSTTLPPPTGSAIEPEASGSPSATEPKKYISLDCEMVGVGGPTNERSALARVSIVNYHGHIILDTFVRPKERVTDWRSWVSGVTPAHMIHAREFEDVQKEVSAILADRVLVGHAVKYDLEALLLSHPRRDIRDTSRHPGFRKFSAGRTPRLKKLALEVLGIEIQGGEHSSVEDARACMLLYRKFRDEIEKLHKPKAPKGASAKSGGGNGKRKRKRKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8F5</accession>
    <name>D5G8F5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00004800001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430042">
        <property type="protein sequence ID" value="CAZ80798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836607.1">
        <property type="nucleotide sequence ID" value="XM_002836561.1"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G8F5"/>
    <dbReference type="GeneID" id="9186018"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004800001"/>
    <dbReference type="HOGENOM" id="CLU_254801_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8F5"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="334"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="405"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="441"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="590"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="662"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="838"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="889"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="939"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1074"/>
            <end position="1199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1268"/>
            <end position="1326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1347"/>
            <end position="1393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="38"/>
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        </location>
    </feature>
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        </location>
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        </location>
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
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        <location>
            <begin position="445"/>
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="851"/>
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        </location>
    </feature>
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        </location>
    </feature>
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        <location>
            <begin position="948"/>
            <end position="957"/>
        </location>
    </feature>
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        <location>
            <begin position="993"/>
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        </location>
    </feature>
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            <begin position="1019"/>
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        </location>
    </feature>
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        <location>
            <begin position="1032"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1087"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1121"/>
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        </location>
    </feature>
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        <location>
            <begin position="1147"/>
            <end position="1163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1165"/>
            <end position="1174"/>
        </location>
    </feature>
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        </location>
    </feature>
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        <location>
            <begin position="1302"/>
            <end position="1315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1372"/>
            <end position="1386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1393" mass="149866" checksum="5D1FEFD7F0114D0F" modified="2010-06-15" version="1">MARRRTKSMAEASNSPPPTAIARATRSRKSLALSSLLETQQPLLMTFEQGSTGQRRSSRSAPKRGEGLALSPTTVVSPMVTRGKQAKDESDGKGKDREGEVSRDHWRSPENSTKRTATPPPILDARDDDEESLVDEADKKDEEHLDEDRQDDDSDYGHRDEDEGNVLPGPLSTNSSPDGQGSYASALNGGDGVSSPSASRNCDTASAKRRRKKTRNPHISPNKQRQLRQKQKEREEKRKGDIVSSPVSAVGSGSGAFPHLPHPPVPITTEAIHAPTSPPPPSAPPEVNRAFEEDFGPFLNGKTDTHEATKRPAMLVDVSSVDAAAEFLLGTAMGGFSEDESGKRVTGNSGAETGQNATMVSEPRDEDMPDAPVQSGASLLDVAPTNDAIVESPSNQLLAELQKNGDDATEGTLSGQASPIIAGASTPATTVNGLAGSRRALCTGGESSPRCQTSPVRRLECSGTSGATEKDISNISSGRRLSLGPGHADISERNNANTGDNTIKENNTKSRESNTRLVVRDGPPSPKLERRDTEIVVGGIGSPTKIASPRERIEIRVQLEGQDPDSGAADEVNVAASVLLQVLKSNNPVSAIRKKRSHSEISSRGRRGNSPGHGESSNKLSCPTCPITEEQILSCLERKNAGDRGMSLEEFMDKHRELCANNVQDEPLVSEGEMEEEDDYGDYDEEEDEGEEGHAGEDHEELGGEEEDYAVDGDEGLIEGDQGVIRKIAPAPSGVGTVLCKVAESLPGYLSIKPEDVDKEIEVRRSAITAGRLLEGKSVEDILLVPITPISTFQRYLENSKTPAEKFLVEEKIVEALADIQVEYLMIDEIVKRKEASTPDGESIAVSQTARGRKKGKPRVARNPLEPEDPVVWEDKKEAELYGFSYDPAIDKRGKQNADEQQAGKYSIRGRELRSRQKAVEMVLYPGYEHIDQRVQTIQPKVTRRRKPAADKEKCEESLEQLRAGSAPPPASAVPQKKRPGRPPKNAQSSLFLSASSKDQASAQALSRTHSADNVARPRAGEKPKVDPNRTPPSSISGTLVRSSPDCLKIKFRGARLAEYLNIVSQESNLASTQRDFYGRPGPSSLVPATTQSEPVSPTRFYPDNPTRQGDSFMSLVNGGVNAQRSESPIYDSTESNPGDYEGVGRGRPRPRGRGRARGRARGTRGPGRPGRGSGLRVETSSAANPVGRGEPVPANPETPALSAVEYSEAEDAAISAAASDRGHNYHSNAFQALSKAPGSAKRSIPAVMHFNATPSPMYTVFQTRLGPHPLPSTSTSPTYHENRSSVETDHGRPRVRGKTYKTPTSVSVTEAQPRTTKEEEFIAAQNGNLGKRKRVRTALAYEAPRRKARAVAGRDGAVGGGHSPGCVSGDRDDETDSSEPDDDDSGGYGNEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8K4</accession>
    <name>D5G8K4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00002983001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430047">
        <property type="protein sequence ID" value="CAZ80847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836656.1">
        <property type="nucleotide sequence ID" value="XM_002836610.1"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G8K4"/>
    <dbReference type="GeneID" id="9186215"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002983001"/>
    <dbReference type="eggNOG" id="ENOG502QUWF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_238722_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8K4"/>
    <dbReference type="OMA" id="FMIQMKS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056416">
        <property type="entry name" value="DH_2_fung"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056222">
        <property type="entry name" value="PH_23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056223">
        <property type="entry name" value="PH_24"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24340">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24344">
        <property type="entry name" value="PH_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24345">
        <property type="entry name" value="PH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DBL homology" evidence="2">
        <location>
            <begin position="596"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="818"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="4">
        <location>
            <begin position="1416"/>
            <end position="1534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="294"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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        </location>
    </feature>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1614"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
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        </location>
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        </location>
    </feature>
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            <begin position="367"/>
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        </location>
    </feature>
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        </location>
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        <location>
            <begin position="480"/>
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        </location>
    </feature>
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        <location>
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    </feature>
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        <location>
            <begin position="1258"/>
            <end position="1269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1277"/>
            <end position="1289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1315"/>
            <end position="1324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1352"/>
            <end position="1373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1614"/>
            <end position="1628"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ80847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1775" mass="193281" checksum="3A76682E29B1ED0D" modified="2010-06-15" version="1">MERHSSPVSIKQTEPESGSETESMSTTESDPESEKEPQPPTKPMFPLETNSAPTTPTRSKSRRINRPMTPEDPLLEFLGRRGTKRGTSPQRMKSERRRMWEKLASGGETSPLPYEATTPSPDPVKRRESRKVKRDDSTPISSPSSPPVKEEHLPKDEEARLPPEIPERPKRTRAEARRTTLTAPAEEPVAETGEFSVSAPATPIPHPMEDLSLRRRKAKSKRLSVQDDGQAKVVEARTDTPSPEPLSFKQAELSVDPAPRKRKSDISTGTFGPKLASATSSFFKAVKAEFQAELQAAAKAAESTSSSEGEDLDLLNHFVENSRPVESIGEQDEGSDGDHEIATDPVTAEELTPPPPTLVARATYTETETETETETETESESESETGSDSRSGSGSQATQEHQPGADPIRDTEVSSESRAQVGSQPELEASAGRKPPTTESLRPSGNVLLGDSQTLLPKRSIRSSPSPPKPKRRGSRARSQGTHTPVSSIDSGEARAYTDNGSVREPSDASLSTVKPLNIKPRDPSPEGQTSPTQAPQGQGGEIYYTPSLLTQEASLKRKYTKHADLISILSVPKPSKSLRSATRTRSHGKMSRKVSTITIQDLMAELATEEAKYLRELRTLVEDVVPVLFQTVLERADDVVRRRSLSGGKPDNYGFRTGFATNPTRPIVDMGISLERLKTVHERMPKQNPNELLIWAMDARKVYEEYLSVWRMGFQDVVVTLAPDYEDEKEFRDYQEELTRKGLEMLYNEGEKVDVAFLLKRPLVRLKVLAKLFKRMNLLQPSAPAQALTSAFHGVVAIARRKIAEEKARIEDEAAASISVANVSDIHTLLPQYDVSLDPTKRVRARDGFYMRLYHSSGRVLQRNVELILRDPPGKARPERTEILVCQFGEGEVSDKWLLFAPIPLGAISARTGDAAGEIVVMVRGMDPSSQEELREILILGSPTAAGFEWVQMLGLIPIPPEGPFTDKPYQMHTLETLTEEGTLSSRWQSPAPPHSSHGPKTPKALSQKSEQETQKKSEKSESVGDVFSFLESDRATPKQEPAPLVVEPPKTVKPPLENASGAEGTNGPKPAEHCEVLPPDFAMMPCIAGGYWFLGGHFKTPEAKTDAEQGLGAQDLLASPPLRHSASLHTLSDRYSAPSEGRSPSLQRSRKASKPSSSRRNSTINNDPDTLSLGPSEPSNYLAPAPTNTLSRTRSIHLDDEARSTAALEKPALRSPFEDAPNLSPDPKPEELGSLTPVKKGTKTDVPPPVPPHRVLTTSQSRDSNPPSRSPVILSMPSNSSPSSGKSGFNGRRRTSSPLKHEYDPSSPSGSESESEEEEEKEEINLQFKEKGNNDETADDESVSSSSEESSSEEEESDDVVSICSEEEDGDYPPPMLSIPRRVSKEPSGIISLPRQRPSSLTLASSAPTIQASAPPAYKFRAWVFAWAINQWEKLHSNECRIIITPGHIESFPSGSPPSTPGRSTTPVFSLDLSPVTPVRRGTAVDVSIRTPPNSKFSGASLMLRCRSPAECETLYNAINNSRVYPVNYQAPSTLLSSLASSEMGISEASSTTAGGSVKRGWGSWGRSKTYRNGIADSNSGSIISSGPSETSVGSLSSAFSRFRQHNRMFKSPLSSVASSSSSNAGDSRRGSPSALSMPVVDGNPVFKIRLYRRENASKWRDLGNSRLSILKPVNASQKQPNSLSTGDEKRIVVTNKAGTLVFLDEVLGESSFERVARTGIAVSVLVGEGEENGTGVPGGVGGIGAKSIVYMMQMKGEAEAAYTFSIVGKLRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8Q4</accession>
    <name>D5G8Q4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00003087001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430050">
        <property type="protein sequence ID" value="CAZ80897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836706.1">
        <property type="nucleotide sequence ID" value="XM_002836660.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8Q4"/>
    <dbReference type="GeneID" id="9186265"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003087001"/>
    <dbReference type="HOGENOM" id="CLU_2348205_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8Q4"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="97" mass="11092" checksum="DAAA0D45DC03B62C" modified="2010-06-15" version="1">MAYLASSPPSLPFFLLFLLVLFVGAILRKMFKNLCFCLSILCFRDVSLYPLLYLSILKSLFVFWYLHLYLFGNGGLVSARQTIGTEGEREGEERSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8U0</accession>
    <name>D5G8U0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="14">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00004835001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the ATP-dependent dehydration of threonylcarbamoyladenosine at position 37 (t(6)A37) to form cyclic t(6)A37 (ct(6)A37) in tRNAs that read codons beginning with adenine.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HesA/MoeB/ThiF family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430053">
        <property type="protein sequence ID" value="CAZ80933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836742.1">
        <property type="nucleotide sequence ID" value="XM_002836696.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8U0"/>
    <dbReference type="FunCoup" id="D5G8U0">
        <property type="interactions" value="177"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G8U0"/>
    <dbReference type="GeneID" id="9186442"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004835001"/>
    <dbReference type="eggNOG" id="KOG2018">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013325_9_3_1"/>
    <dbReference type="InParanoid" id="D5G8U0"/>
    <dbReference type="OMA" id="GSWVVTM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061503">
        <property type="term" value="F:tRNA threonylcarbamoyladenosine dehydratase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008641">
        <property type="term" value="F:ubiquitin-like modifier activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061504">
        <property type="term" value="P:cyclic threonylcarbamoyladenosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00755">
        <property type="entry name" value="YgdL_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000125">
        <property type="entry name" value="tRNA threonylcarbamoyladenosine dehydratase 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43267">
        <property type="entry name" value="TRNA THREONYLCARBAMOYLADENOSINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43267:SF2">
        <property type="entry name" value="TRNA THREONYLCARBAMOYLADENOSINE DEHYDRATASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="125"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="13">
        <location>
            <begin position="107"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ80933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="496" mass="54893" checksum="A8F1A04D13025D1D" modified="2010-06-15" version="1">MVYANLNLHNFNLTLHSHRAQLAYTALVSGAVVASTIFAFQRVKQISRLKRLKSLKDSIPPPSEVNVLTNFGAASARSRLQDESDAVLAANARRGDWDEDIILEQLARNRIFLGEEGLKKVRNSFVIIVGCGGVGSWVATMLLRSGVGKIRLVDFDQVTLSSLNRHAVATLADVGTPKVQALRKRLEQVAPWVEIDARNELWEMEDGAALLAGEPTFVVDAIDNIQTKVDLLFYCKTHNIPVVSSMGAGCKCDPTRICIGDISESTEDPLSRSTRRRLRQKGVSTGVPVVYSTEKPGPGKASLLPLSEDEFEKGKVDELSVLPDFRVRILPVLGTMPSIFGLCIASHILTAIAGYPTEYSKGKDRSKLYEDMIAQLAGQESRLRGNAPGIRIPLNERDAGYVADEVFKGKSVVSGFFTRLVLTRWEPLPPRDEGFWGENDHTVTTNDVVLMTKDEAKKHEKEVLKAGRSPEDVWGPHVVGVVEERRKEEDFYSKFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>D5G934</accession>
    <name>D5G934_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00004965001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430053">
        <property type="protein sequence ID" value="CAZ81027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836836.1">
        <property type="nucleotide sequence ID" value="XM_002836790.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G934"/>
    <dbReference type="GeneID" id="9184314"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004965001"/>
    <dbReference type="eggNOG" id="ENOG502QSI5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_475022_0_0_1"/>
    <dbReference type="InParanoid" id="D5G934"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="476"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="421"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="259"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ81027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="574" mass="60459" checksum="08FBB1BF6EE06924" modified="2010-06-15" version="1">MNPRRGIYQNNADMSTFLDWTLPGPGTGVIGGQKAEGASSLIPEPGMHVHHLETIDQSAFLALPVAQSAKTLSLPSDAGSGENEPHAYDAARPVGQRRSEAMGGRGGETENPGNTRGSGGAGGGAGGSDHSDSSSDGDGDGGRGNRGSNRGGNRGGNRGGNRGGNRGGNRGGNRSGGRGGGGRDGVAGGAGGSNHPDSSSDGDGDGGRGNRAGGRAGGRGGGRAGGRGGEKPGDPSSGDDDGRGSRGSRGRGGRGGRGGGRDGKEPRDFRVEDERGVWVGQFRFTGIDGEYIECIRTTLETRAHLKDLADKPRLTLADVEFLTNFINQQSTPPDQAILREIWGLAPRAERAARRTLREEAWPARGEKAAFGLVSVNSPFGILAWPWRQLWDIAFSLDDADYQLPGTNAFQDYINEKRMRTTRGGNGDDSVNPARAAQGQSGIDPEISAKRIYSTLPRRPHQTPVASILRAGPKPGAAWGILTFLGLGLVLTLAIYNSLIFLRMRSDQHSWEFANGSRSSAGPFWSVENSCWSNCLSGIWGGPSQSSRKSQQWEWLWTLLEDWVATNDEGQILPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>D5G975</accession>
    <name>D5G975_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00003182001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430056">
        <property type="protein sequence ID" value="CAZ81068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836877.1">
        <property type="nucleotide sequence ID" value="XM_002836831.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G975"/>
    <dbReference type="STRING" id="656061.D5G975"/>
    <dbReference type="GeneID" id="9184354"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003182001"/>
    <dbReference type="eggNOG" id="ENOG502RZ2M">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028123_3_1_1"/>
    <dbReference type="InParanoid" id="D5G975"/>
    <dbReference type="OMA" id="PAGWRNG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050464">
        <property type="entry name" value="Zeta_carotene_desat/Oxidored"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42923:SF42">
        <property type="entry name" value="AMINE OXIDASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42923">
        <property type="entry name" value="PROTOPORPHYRINOGEN OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13450">
        <property type="entry name" value="NAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="439" mass="48495" checksum="296E40EF16D9AB92" modified="2010-06-15" version="1">MEAQSPQKKRVIVIGSGLAGLTVAYLLHNDPKCEYDVEIFESGSHPSIDATTLPTNLEIDIPMRTFSKNYYRTLHTLYKHLNLPIHLQKFTFIFATLSSGQSRPKIYYTHPSNFHAFPAPTPGVNPLSHIMTLVVLYLAYIYFTLCIFLLHPSEDETVSSWLRRSRLPEWVVKDYFLPMFGIVGTCSSAEVLSSPVHDVIAYRRGMLFRRHYVVGGGVGVIRQRLLHGIPIQLNRRVRRVCKTPRGLSINWHGGDGEEVADAVVFAVPPDVLAGIYAPLAPSLSQLPTTKVKVYAHTDCTVLAPSYSLLSRSFSPAKDHVAVQLTTSNKRTSATEVHAGVMVSTTPQLVPEKDKVVVEVEYTRVLRTPESRRIVNALLDGDGGEGEGWRNGDGGVWVVGGWCWDGMVLLEGCVKSAVRVWEGGFGGRIEDLGRVGSGLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>D5G983</accession>
    <name>D5G983_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Arginase</fullName>
            <ecNumber evidence="2 11">3.5.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00003192001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 11">
            <text>L-arginine + H2O = urea + L-ornithine</text>
            <dbReference type="Rhea" id="RHEA:20569"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16199"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="EC" id="3.5.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="11">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9 10">Belongs to the arginase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.3.1" evidence="2 11"/>
    <dbReference type="EMBL" id="FN430056">
        <property type="protein sequence ID" value="CAZ81076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836885.1">
        <property type="nucleotide sequence ID" value="XM_002836839.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G983"/>
    <dbReference type="FunCoup" id="D5G983">
        <property type="interactions" value="1250"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G983"/>
    <dbReference type="GeneID" id="9184362"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003192001"/>
    <dbReference type="eggNOG" id="KOG2965">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039478_6_1_1"/>
    <dbReference type="InParanoid" id="D5G983"/>
    <dbReference type="OMA" id="YKEFRYA"/>
    <dbReference type="UniPathway" id="UPA00158">
        <property type="reaction ID" value="UER00270"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004053">
        <property type="term" value="F:arginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006525">
        <property type="term" value="P:arginine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000050">
        <property type="term" value="P:urea cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd09989">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.10:FF:000012">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.10">
        <property type="entry name" value="Ureohydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014033">
        <property type="entry name" value="Arginase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006035">
        <property type="entry name" value="Ureohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020855">
        <property type="entry name" value="Ureohydrolase_Mn_BS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01229">
        <property type="entry name" value="rocF_arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43782">
        <property type="entry name" value="ARGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43782:SF3">
        <property type="entry name" value="ARGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00491">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00116">
        <property type="entry name" value="ARGINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01053">
        <property type="entry name" value="ARGINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51409">
        <property type="entry name" value="ARGINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0056">Arginine metabolism</keyword>
    <keyword evidence="6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 11" id="KW-0464">Manganese</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ81076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="346" mass="36567" checksum="4E4F0C148B371895" modified="2010-06-15" version="1">MTAPSTIATKFLPAKDQLGLVAVGFSGGQCKSGVDAAPSALIESGLLADIQETLEYTLYHDGVVHGYAKHLPVPGTDLPLRTAGAKAPMINPIAVSSVTHALCDQVYEHASKGRLVLTLGGDHSIAIGSVAGTAKAIRERHNGSEMALVWVDAHADINTPESSGSGNIHGMPVSFLTGIARPPKENPDVFGWIKDEHRVNPSKIVYIGLRDVDIPEKKILKDHGIRAFSMHDIDKHGIGKVMDMALDHINCGPGQEGMPIHLSFDVDALDPYVAPSTGTPVRGGLTLREGDFICEVIHETGYLVAMDLVEVNPALSGDGPESISARETIRAGCSLVRCALGECLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9I1</accession>
    <name>D5G9I1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00003411001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430061">
        <property type="protein sequence ID" value="CAZ81174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836983.1">
        <property type="nucleotide sequence ID" value="XM_002836937.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9I1"/>
    <dbReference type="FunCoup" id="D5G9I1">
        <property type="interactions" value="238"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G9I1"/>
    <dbReference type="GeneID" id="9184247"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003411001"/>
    <dbReference type="eggNOG" id="KOG2777">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005382_2_1_1"/>
    <dbReference type="InParanoid" id="D5G9I1"/>
    <dbReference type="OMA" id="PDIKIYM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043829">
        <property type="term" value="F:tRNA-specific adenosine-37 deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002100">
        <property type="term" value="P:tRNA wobble adenosine to inosine editing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002466">
        <property type="entry name" value="A_deamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042935">
        <property type="entry name" value="Tad1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47803">
        <property type="entry name" value="TRNA-SPECIFIC ADENOSINE DEAMINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47803:SF1">
        <property type="entry name" value="TRNA-SPECIFIC ADENOSINE DEAMINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02137">
        <property type="entry name" value="A_deamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00552">
        <property type="entry name" value="ADEAMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50141">
        <property type="entry name" value="A_DEAMIN_EDITASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="A to I editase" evidence="1">
        <location>
            <begin position="55"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="408" mass="44029" checksum="A743910C245F9974" modified="2010-06-15" version="1">MDSLEDSVASAVLSAYVDLPNKSKPLGRGGINEWVPLSGIVLECGEGTSRLTAVALGTGMKCLPKDKIPFAGGMVLHDSHAEMLAIRGLNCFLLEECQSLSADRLFVSKFIRRRSQQEISQQSPQPFTLRESVLIHFYSSEAPCGDASMELTMAVQDDPTPWTILPLAPASSTSPRALGQPRTLLQGRQYFSELGFVRTKPGRTDSLPTLSKSCSDKLSVRQVISALLSPTTLLISPENAYIMTVILPTTSYCPNACARAFSTTGRMAPLVDRKFEGGYKFSPFAVRTTNLSFGFSKRVIGTGAIGSNISAVWVKGRSVETLIGGVLQGRKQFASGIKGASMICKMMIWRLAQKVAEAEGIDGVGGERYGSVKLGVKMVARNEAKEMTREVLGGWVKNGGDDFGNVRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9N5</accession>
    <name>D5G9N5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00004991001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430064">
        <property type="protein sequence ID" value="CAZ81228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837037.1">
        <property type="nucleotide sequence ID" value="XM_002836991.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9N5"/>
    <dbReference type="GeneID" id="9185365"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004991001"/>
    <dbReference type="eggNOG" id="ENOG502S8A9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_649224_0_0_1"/>
    <dbReference type="InParanoid" id="D5G9N5"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072251" evidence="2">
        <location>
            <begin position="22"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="109"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="318"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="318"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ81228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="423" mass="41891" checksum="59DE8C49F0FA8E19" modified="2010-06-15" version="1">MHTSKSLSLLAVALMQGLAAAAPQGVHYHMNGTITATGTSTGTSSSSSVASPIGTAPSHSFSIPVPMGTAPSHFSSVPVSLGTAPSHSSSVPVPMGTAPSHSFSVSVPMGTAPSHSSSVPVSMGTAPSHSSWVSTPIGTAPSHSSSVPVSMGTAPSHSSWVSAPMGTAPSHSSSVPVPIGTGTGSSRSSVPITASSSTIAHGTSMVDGFTTTLSTMTKTKTLTWTVMVPCTTVAAGTTSYYTTASVYTTYVSDLKTITITSACTKCAYATPTPAGPGGSVAPDTTIIKPADSTPTSSVKIIQEIKTVVPIVVSAGQTTTAPAASVPSNPTGPAKGTTSGFSGSGVCPAVSTVTVYNPTTIYATVTVSPGVSTPSTTVVGCSTGKCSTITNVYAKTFTNWNGTEPTVAFKPTGKGFATGLTTDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9Q1</accession>
    <name>D5G9Q1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00005017001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430064">
        <property type="protein sequence ID" value="CAZ81244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837053.1">
        <property type="nucleotide sequence ID" value="XM_002837007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9Q1"/>
    <dbReference type="GeneID" id="9185381"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005017001"/>
    <dbReference type="HOGENOM" id="CLU_598775_0_0_1"/>
    <dbReference type="InParanoid" id="D5G9Q1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="363"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="65"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="379"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="457" mass="48901" checksum="53F40AE63F7FFDA6" modified="2010-06-15" version="1">MDIPALPLFDAIRGVMAGSWPDDEYDLAESPLSYVDYRKQKLDCGEPVTITTPSITIVKQDAEPESVVVVGEPSPESGDSADPAPKAESGPKLGTETSETDIDTKGTGYVDAGVQTDGSWLNAMLGFEAPREKDISASALQDGREEDGRTVLGTEVEEGNNLPTRSRRNTLVQTEGTSHLDPLRPITSGGDSTETLSINSDGPTEVSTAVQVRIFSSPPPSSVTTLPLDRHDSGFFPTPTSRPQTPPLKSKSRRSLPPSPPPSPPSMRRQAVVVGQSLIERKSTESTTTSTSARIISIENPQPSSVSIVSHNFSTTSVTRPRFSTSSLIVPNSLYSSPKSSRFFEDLSDELRPGTAASVEELARQSEPKKAPSVRPCTTVPSVQSEKITTSLVRPATAPGPSTPSKGKHRKLKLRLLKGCGLKIGIPQWAKKKKSSNEGKRVVSYESTVLTLASEKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>D5GA64</accession>
    <name>D5GA64_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="11">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00005152001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for efficient assembly and nuclear export of the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the 66S pre-ribosomal particle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the LOC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430075">
        <property type="protein sequence ID" value="CAZ81418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837227.1">
        <property type="nucleotide sequence ID" value="XM_002837181.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GA64"/>
    <dbReference type="FunCoup" id="D5GA64">
        <property type="interactions" value="304"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GA64"/>
    <dbReference type="GeneID" id="9185700"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005152001"/>
    <dbReference type="eggNOG" id="ENOG502RY6R">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096593_0_0_1"/>
    <dbReference type="InParanoid" id="D5GA64"/>
    <dbReference type="OMA" id="RESMNTI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008298">
        <property type="term" value="P:intracellular mRNA localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037650">
        <property type="entry name" value="Loc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28028">
        <property type="entry name" value="60S RIBOSOMAL SUBUNIT ASSEMBLY/EXPORT PROTEIN LOC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28028:SF1">
        <property type="entry name" value="60S RIBOSOMAL SUBUNIT ASSEMBLY_EXPORT PROTEIN LOC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="109"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="13"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="111"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="181"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ81418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="190" mass="21271" checksum="2291777266557939" modified="2010-06-15" version="1">MAPNPPHPRKPSSSKPTTIAPSSKSAPAKQPKKKFVPKTHTTAGVAIGKNNKKHKDPKKQREALLARLEKELPKLNMVTPAGVVKPKGRKKGKVFVEDREKMMGILNRVSDGVEGRERSKLERARQLEAIREEKRKEEEKREAERNSRMEGAKDAVRKKRKRKSAGAAPEAPKSSSEKTPKGRKKMVTFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>D5GA81</accession>
    <name>D5GA81_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00005176001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430075">
        <property type="protein sequence ID" value="CAZ81435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837244.1">
        <property type="nucleotide sequence ID" value="XM_002837198.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GA81"/>
    <dbReference type="STRING" id="656061.D5GA81"/>
    <dbReference type="GeneID" id="9185717"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005176001"/>
    <dbReference type="eggNOG" id="ENOG502QPU6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003477_0_1_1"/>
    <dbReference type="InParanoid" id="D5GA81"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005740">
        <property type="term" value="C:mitochondrial envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000964">
        <property type="term" value="P:mitochondrial RNA 5'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013943">
        <property type="entry name" value="Pet127"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31014">
        <property type="entry name" value="MITOCHONDRIAL TRANSLATION SYSTEM COMPONENT PET127-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31014:SF0">
        <property type="entry name" value="MITOCHONDRIAL TRANSLATION SYSTEM COMPONENT PET127-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08634">
        <property type="entry name" value="Pet127"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="554"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="592"/>
            <end position="609"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="772" mass="86820" checksum="AC9710FAB07B1A27" modified="2010-06-15" version="1">MIRTALRRAQLSPHSAISPFVCIFCQRKGPNLERRLTTEDVGQSNSVEALPITKASASSSRKPRSLVDILEKDIPERKEGGSRKRGAKKEKAVEKKEAKKAKAGKKKGVSLSKAEAAVSLSPKKKKVFQRAHGSITPGLSISEAFQGDGSKYQLLDPNSREIEPIEREGQPEVARLSHDLDRVLFNPGVHFLQEPRSRVYNFDPYLQEIMPVTEFDFDALASYIISSEDWRLSSLAKELGKKYVGSTSSMTAVLSHFHFLLSQWRPLNFQNLSKTFKAEFQTFTQISRAPAAIFLRYKGNGTYAIDPDKEYDTDSVLSLLGRSMEKLLTSAPSNFESKYKKGNARPEEEPLTKRPETYHYSTLGDILMRSQLDAHDARLPGTGMFDLKTRAVVSVRMDVSNFHIGSGYQIKTRVGTWESFEREYYDMMRAAFLKYSLQVRMGRMDGIFVAFHNTEKIFGFQYVSLDEMDAALHEDWGAGIGDQEFRLSLQLFNKVLDNAVKKYPEQSLHLHFEARDKFRNPMIVVAEPMDEEEVERRQTGNKRDLADFLEALDGQIKSGGGPVPSFKGLEGATSSDEEEDVQNDIQEKEEVNNEEEESESEEDGDESDVGDAKEAEPNQDVEETAEGSKDVAGALKGKLGDDAGARGPVGVSGKKGVEKPIKLPEMYVQVMYIKNFVNDKAVLRPVRLKENDRWRVAVEIDELAPRDAYLINVGVVKRRKKAIEHRMVTEDDAEAGAYIRQLRKMSRAGRDWAKAQEKMFPGEPVIYKPGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>D5GA83</accession>
    <name>D5GA83_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00005178001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0357 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430075">
        <property type="protein sequence ID" value="CAZ81437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837246.1">
        <property type="nucleotide sequence ID" value="XM_002837200.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GA83"/>
    <dbReference type="FunCoup" id="D5GA83">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="GeneID" id="9185719"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005178001"/>
    <dbReference type="eggNOG" id="ENOG502S73R">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128832_1_0_1"/>
    <dbReference type="InParanoid" id="D5GA83"/>
    <dbReference type="OMA" id="NIRDGDS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018559">
        <property type="entry name" value="DUF2015"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28023">
        <property type="entry name" value="UPF0357 PROTEIN YCL012C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28023:SF1">
        <property type="entry name" value="UPF0357 PROTEIN YCL012C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09435">
        <property type="entry name" value="DUF2015"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ81437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="129" mass="14756" checksum="09575ED8FE168FF4" modified="2010-06-15" version="1">MALTDNYMLWTWALFLMLIATGLYFYRSRWLPLISNMIPEHTYRRLNPSFESDIEAGLSSDDFNLSGNLAAGDSRGGLDLASKKEVLRIMKGQRVNFDEARRIFMEQKLLKNGIAADGRPMDPRAVFFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAD4</accession>
    <name>D5GAD4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">ATP synthase subunit d, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00005250001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the ATPase d subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430075">
        <property type="protein sequence ID" value="CAZ81488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837297.1">
        <property type="nucleotide sequence ID" value="XM_002837251.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAD4"/>
    <dbReference type="FunCoup" id="D5GAD4">
        <property type="interactions" value="455"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GAD4"/>
    <dbReference type="GeneID" id="9182523"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005250001"/>
    <dbReference type="eggNOG" id="KOG3366">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080463_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAD4"/>
    <dbReference type="OMA" id="INWAGIT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015078">
        <property type="term" value="F:proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015986">
        <property type="term" value="P:proton motive force-driven ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008689">
        <property type="entry name" value="ATP_synth_F0_dsu_mt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036228">
        <property type="entry name" value="ATP_synth_F0_dsu_sf_mt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12700">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT D, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05873">
        <property type="entry name" value="Mt_ATP-synt_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005514">
        <property type="entry name" value="ATPase_F0_D_mt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161065">
        <property type="entry name" value="ATP synthase D chain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="5" id="KW-0138">CF(0)</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 12" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="8 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7 12" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0813">Transport</keyword>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="102"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR005514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ81488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="175" mass="19758" checksum="CB6934B3210AA018" modified="2010-06-15" version="1">MSAPAKSAALKINWAGITSSLGLKGQTAASLQAFKKRNEDARRKVVVLREQPMKVDFSYYRSILGNQAIIDEVENAWKRFQPVTYDVEKQVRIIEGFEAEAVRNAEVTKGRVDNELKELEKTLRNIEETRPFEDLTVDDIAAAHPDIDARTAEMVSKGRWMPAGYKEKFGELSVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAF8</accession>
    <name>D5GAF8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00005269001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430075">
        <property type="protein sequence ID" value="CAZ81501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837310.1">
        <property type="nucleotide sequence ID" value="XM_002837264.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAF8"/>
    <dbReference type="FunCoup" id="D5GAF8">
        <property type="interactions" value="50"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GAF8"/>
    <dbReference type="GeneID" id="9187441"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005269001"/>
    <dbReference type="eggNOG" id="KOG2634">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027654_1_1_1"/>
    <dbReference type="InParanoid" id="D5GAF8"/>
    <dbReference type="OMA" id="SHIEGWI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043399">
        <property type="term" value="F:tRNA adenosine(64)-2'-O-ribosylphosphate transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019988">
        <property type="term" value="P:charged-tRNA amino acid modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007306">
        <property type="entry name" value="Rit1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033421">
        <property type="entry name" value="Rit1_DUSP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033449">
        <property type="entry name" value="Rit1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31811">
        <property type="entry name" value="TRNA A64-2'-O-RIBOSYLPHOSPHATE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31811:SF0">
        <property type="entry name" value="TRNA A64-2'-O-RIBOSYLPHOSPHATE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04179">
        <property type="entry name" value="Init_tRNA_PT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17184">
        <property type="entry name" value="Rit1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007747">
        <property type="entry name" value="Ribosyl_Ptfrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rit1 N-terminal" evidence="3">
        <location>
            <begin position="66"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="Rit1 DUSP-like" evidence="2">
        <location>
            <begin position="383"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ81501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="491" mass="54156" checksum="0B8E75067ED0E54D" modified="2010-06-15" version="1">MPPSNPSHLKMTPLIATTKTELTSIEPEELDTSSPEPEDLTQYTFTLTPDLLSTIRPTHSSLKNSLRTQTRSLRNRLTSITADSQFVLSVAAAYALPLIPNARAGDWYVPPEHRGSNTSAYFKSTDGHPGQWGFSLRRLNLHILERVGRCGGVVLVDSTRRGKRFPDALSRTVPIWIAVVNWCLFGREFGGLYVSEKMVSGSERVQIERLVPGFVERFLELGVNLSTYKQTIIKPLRPIWVSPESTLPVSPPEFKDFTPIILLTASKMTRGEGMEGEYIQGAGDDHEGWATESGLTPALFWHHHTKIMSTPEQDLLEAIKNIPPLLDNTLGDSKITRIGRTNISIAPTSAIPTLPLTPTFDHIICTSPSPLPPTEGITPMPSHHPIPNNSPGAKLLIKLLPEITSSIVNPAGNILITCDSGEHVSACVALVLLCMYYDDDGEFRKLGEGKGVVDKTYIRRRLAWVTMARDEVNPARTFLNGVNAFLMGRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAM4</accession>
    <name>D5GAM4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00003683001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430079">
        <property type="protein sequence ID" value="CAZ81567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837376.1">
        <property type="nucleotide sequence ID" value="XM_002837330.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAM4"/>
    <dbReference type="GeneID" id="9187734"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003683001"/>
    <dbReference type="HOGENOM" id="CLU_2814281_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAM4"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ81567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="67" mass="7463" checksum="26923755E43325DC" modified="2010-06-15" version="1">MYPPPPPSPWCALRACDVSVPPRGIDSCPRGELNLGAWGRQVCLDRGAVIFCRWGTVRAFGFVLFWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAM9</accession>
    <name>D5GAM9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00003691001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the allantoicase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430079">
        <property type="protein sequence ID" value="CAZ81572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837381.1">
        <property type="nucleotide sequence ID" value="XM_002837335.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAM9"/>
    <dbReference type="STRING" id="656061.D5GAM9"/>
    <dbReference type="GeneID" id="9187739"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003691001"/>
    <dbReference type="eggNOG" id="KOG4145">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_987619_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAM9"/>
    <dbReference type="OMA" id="HIEPNIF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004037">
        <property type="term" value="F:allantoicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000256">
        <property type="term" value="P:allantoin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005164">
        <property type="entry name" value="Allantoicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015908">
        <property type="entry name" value="Allantoicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12045">
        <property type="entry name" value="ALLANTOICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12045:SF3">
        <property type="entry name" value="INACTIVE ALLANTOICASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03561">
        <property type="entry name" value="Allantoicase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Allantoicase" evidence="2">
        <location>
            <begin position="22"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="Allantoicase" evidence="2">
        <location>
            <begin position="113"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ81572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="282" mass="31185" checksum="DDFD816E4F396225" modified="2010-06-15" version="1">MVSRLTHLASTKTTFILTPKPFRWEGILTMRECGPSRRHIWKLSSPTTKAYSHVRLNIFPDRCNLITDSPTTNTGSTNTLLGIPRFRLFRTTVSVFSSDPDAIIDFALVSFDGLAISYSDRNSGIKAANLLLPGRGKDAGNGWETNRSREPGHVDWVRQGCTPFPWTSRNALVILTGFHHKGAPGYIEEIVVDTLHFRGNYSQAVEIYTINSSDQHIAGDASGWELIVPAHSCEADKEHAFQSTLFQNINGKTVTHVKMAIILDGGVKRSRVFGKSAIFAGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GB71</accession>
    <name>D5GB71_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00003772001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430087">
        <property type="protein sequence ID" value="CAZ81764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837573.1">
        <property type="nucleotide sequence ID" value="XM_002837527.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GB71"/>
    <dbReference type="STRING" id="656061.D5GB71"/>
    <dbReference type="GeneID" id="9181474"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003772001"/>
    <dbReference type="eggNOG" id="KOG1039">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018150_0_0_1"/>
    <dbReference type="InParanoid" id="D5GB71"/>
    <dbReference type="OMA" id="YDMIPTI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045072">
        <property type="entry name" value="MKRN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11224:SF10">
        <property type="entry name" value="IP09428P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11224">
        <property type="entry name" value="MAKORIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="84"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="113"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="84"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="113"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="180"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="301"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="394"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="544"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="25"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="333"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="394"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="545"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="558"/>
            <end position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ81764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="615" mass="65319" checksum="F131CD22AF208D2F" modified="2010-06-15" version="1">MYNSGSSNPSGSNSQGHSRPGSGRRSISASQPLSGQLPPTQNGNAVAGTASTPVPQSHAQPPGIPIGPNMPTFDASRSPPSSKNLNHVPCKFFRQGTCQAGKACPFSHSMDPTSDQAPCKYFSKGNCKFGVKCALAHILPDGRRINRPTYSSSSHLQFGRVDTGLTNQRSALHNSLLQANMLPPNAPNHSFSSQDDFPGLTSHPGGPDPNLTTSILDSRYGSPRDDGTLPMSPLASSQRTLGPLDATLPASFDRHEYSKFARFGPYASSVPSKFGMESPPPSLPGNTRPAALQSLYASAYGGNAEEDNNPGADLSHSPRANTEDSLGPRAMHSSRSLSRMKSLSSSYARSENMERMLGEFTRERSPIDPTVDGNFAFEEDFVPSSLQELLTPAERNRRLSRTDEEGVSNFRHSLTAPGTPGDSMNVIGSPPRPSSGWGPIVTRNKRDEDHAPTSGVGHVGSPLRNSYQQGGEPSPTFRPLGRPVSGDVSPFPGSPNRTSSGISALTQTLQRARLSSGSGPYGPGEAPTLSKALGSNGKQMERVISNPQSNNGKTVASIDEESDTQFPMEEEEYSTSPRLAISFGSLGAIGRGSGLGLRVSEQQWESFGTGTKKFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBA0</accession>
    <name>D5GBA0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00000534001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="FN430089">
        <property type="protein sequence ID" value="CAZ81793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837602.1">
        <property type="nucleotide sequence ID" value="XM_002837556.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBA0"/>
    <dbReference type="STRING" id="656061.D5GBA0"/>
    <dbReference type="GeneID" id="9181504"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000534001"/>
    <dbReference type="eggNOG" id="ENOG502S2CW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021264_11_3_1"/>
    <dbReference type="InParanoid" id="D5GBA0"/>
    <dbReference type="OMA" id="YWEDFEY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10951">
        <property type="entry name" value="CE4_ClCDA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.370">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002509">
        <property type="entry name" value="NODB_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46471">
        <property type="entry name" value="CHITIN DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46471:SF2">
        <property type="entry name" value="CHITIN DEACETYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51677">
        <property type="entry name" value="NODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-0170">Cobalt</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="NodB homology" evidence="8">
        <location>
            <begin position="46"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="252"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="269"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ81793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="488" mass="51389" checksum="D4FA1E611076A656" modified="2010-06-15" version="1">MSPLCQLGYGICDADTLPSGGTTEQVARPHVGTVPYNIFDDCIIPGDVALTFDDGPGVYTNGMVDLLDSFNAKATFYITGINNGKGAIDTTSAWADVIRKMNANGHQIASHTWSHADLSTLSEAGRRGEMIKLEMALRNILGFFPTYMRPPYSSCNAACVATMSALGYHITYFDLDTDDYNQNTPQKIQVAKNNFDNAINPSNPAADAFLAIAHDIHQTTAQNLTEHLLRTLQQKGYKAVTVGECMGDPKENWYRAAGPGPSFTSEIVTPTSQPTTSTSQSSTSTTSPTSSSSSSSTTSTSSSSSTTSTTSTTSTTSATSTTSTTSTTSTTSTTSTTSTSSTSSSSSSSSTARPATRSTITTTSSTSTPAPTTTTTSSPTSSTPKPPTSTTTTPSTTTSKPPSPTSSCAFLVGRWCAEPLPTYNNVEGCMASTNKCWKQGNYCWLATDSMSCRYYLGVCLAQEEHCTSCQEAGSTSCAYVTPVYPKPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBB6</accession>
    <name>D5GBB6_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Proteasome subunit alpha type</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00000405001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the peptidase T1A family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430090">
        <property type="protein sequence ID" value="CAZ81809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837618.1">
        <property type="nucleotide sequence ID" value="XM_002837572.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBB6"/>
    <dbReference type="FunCoup" id="D5GBB6">
        <property type="interactions" value="929"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GBB6"/>
    <dbReference type="GeneID" id="9181520"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000405001"/>
    <dbReference type="eggNOG" id="KOG0176">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035750_4_2_1"/>
    <dbReference type="InParanoid" id="D5GBB6"/>
    <dbReference type="OMA" id="RSMIDHA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034515">
        <property type="term" value="C:proteasome storage granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03753">
        <property type="entry name" value="proteasome_alpha_type_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000015">
        <property type="entry name" value="Proteasome subunit alpha type-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033812">
        <property type="entry name" value="Proteasome_alpha_type_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003075">
        <property type="entry name" value="PRK03996.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 5 6" id="KW-0647">Proteasome</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="7">
        <location>
            <begin position="8"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ81809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="245" mass="26805" checksum="2C4DDC7305D2621C" modified="2010-06-15" version="1">MFIARSEYDRGINTFSPEGRLFQVEYSLEAIKLGSTAIGVATGYGVIIGVEKRVTSPLLEASSVEKIVEIDRHLGCAMSGLQADARSMIEHARVEAQNHSFHYNEPLRVESCTQAICDLALRFGEGADGEESIMSRPFGVALLIAGYDEDGPQLYHAEPSGTFYRYDAKAIGSGSEGAQAELQNEYHSSLTLEEAETLVLKTLKQVMEEKLDSKNVQLASVTKEKGFKIYKDEEMAEVVARLGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBF3</accession>
    <name>D5GBF3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00000446001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430094">
        <property type="protein sequence ID" value="CAZ81846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837655.1">
        <property type="nucleotide sequence ID" value="XM_002837609.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBF3"/>
    <dbReference type="GeneID" id="9184435"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000446001"/>
    <dbReference type="HOGENOM" id="CLU_2795783_0_0_1"/>
    <dbReference type="InParanoid" id="D5GBF3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ81846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="68" mass="7604" checksum="AE3F9362E2012240" modified="2010-06-15" version="1">MPALGFLKKKKKEKDSFGTIDANLRHYTGHFPSLIQAFDCVSFLDFNCPSNDPICSLCSCRRIHGIGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBI0</accession>
    <name>D5GBI0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00000507001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ELP6 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430096">
        <property type="protein sequence ID" value="CAZ81873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837682.1">
        <property type="nucleotide sequence ID" value="XM_002837636.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBI0"/>
    <dbReference type="STRING" id="656061.D5GBI0"/>
    <dbReference type="GeneID" id="9184446"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000507001"/>
    <dbReference type="eggNOG" id="KOG4723">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059771_1_0_1"/>
    <dbReference type="InParanoid" id="D5GBI0"/>
    <dbReference type="OMA" id="CISCRPL"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018627">
        <property type="entry name" value="ELP6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16184">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16184:SF6">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ81873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="245" mass="25812" checksum="B2B69C96BFB71470" modified="2010-06-15" version="1">MPPQTPLTPYISPPKNPHTLTLLTSLLASPSSWLTHALLATHLTSDASVVLISLTQDRAFHVDGLRKLGVPLHHLDRSEKFTFLGITDSTSALTRSFSTTVLAAVESAARVSGGRGEGVVVVLESLDLLLQIGGEGGYVGVMDLVLREQEVAEHTVVSVNVDEALMRDEEHAAFVIGLGHVARRVFGLRGLETGVARDVSGVLRITAGGVGEDEGEGDGGGKEVLYFVGDGGGVEVFERGEVRGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBJ9</accession>
    <name>D5GBJ9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00005669001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ82005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837814.1">
        <property type="nucleotide sequence ID" value="XM_002837768.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBJ9"/>
    <dbReference type="STRING" id="656061.D5GBJ9"/>
    <dbReference type="GeneID" id="9185440"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005669001"/>
    <dbReference type="eggNOG" id="ENOG502QTQR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016815_4_0_1"/>
    <dbReference type="InParanoid" id="D5GBJ9"/>
    <dbReference type="OMA" id="RIGECFR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040151">
        <property type="entry name" value="Gfd2/YDR514C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048519">
        <property type="entry name" value="Gfd2/YDR514C-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28083">
        <property type="entry name" value="GOOD FOR FULL DBP5 ACTIVITY PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28083:SF1">
        <property type="entry name" value="GOOD FOR FULL DBP5 ACTIVITY PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21762">
        <property type="entry name" value="DEDDh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gfd2/YDR514C-like C-terminal" evidence="1">
        <location>
            <begin position="173"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF21762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="487" mass="55095" checksum="D2FD68624964DA28" modified="2010-06-15" version="1">MNSFVEYKTLKSFPYKFLTKADQHVVSRFFDSNPFHAYGWRIYIYETAVSETILIPWDQIDRFFKEIAAETGIHLFQNFPAKKISYKLDSNEDAPALMGDFKSKEELDELLQQFGTSSGKGPIKKNSTPKSRRNAARRLQAMETWKSQVSSARNHLKYDQQDLENGVKIAFPVFVSIDVEAFEHNHNIITEIGISTLDTAELPPTQENAMTRETVLKAIQDSFGLSNAPLRRSDAVIDLIKSYHFRVSENRNMRNGQFVTDAADYFMFGDSEFVSLTELPKRIGECFRHFDSHGEKRGIVLVGHDVKTDVDFLMAVGYNVGNISGLEVIDTTCMWKAVMGDFQSKGLGSMLHDLSIDFRHLHNAGNDAAYTLQALVKMAEIGLPIEGGNGDNVKPAPNLYRPIWQKAENIQIGFADEDDNRPANILPPKKPIAKYVAEAGSSSEGSGSEHERRWLGLTARQFPETLFCCDFHLFFFSLSKFSHYHLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBL1</accession>
    <name>D5GBL1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="11">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00005684001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
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            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="11 12">Mel28</strain>
        </source>
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            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
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        <text evidence="2">Belongs to the syntaxin family.</text>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
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        </submittedName>
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        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
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            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RRM2_Hrp1p"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="RNA-binding protein Musashi homolog 2 isoform X1"/>
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        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR48032:SF6">
        <property type="entry name" value="RNA-BINDING (RRM_RBD_RNP MOTIFS) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR48032">
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        <property type="match status" value="2"/>
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        <property type="match status" value="2"/>
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    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="168"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="252"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
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        <location>
            <begin position="468"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="27"/>
            <end position="72"/>
        </location>
    </feature>
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            <begin position="328"/>
            <end position="365"/>
        </location>
    </feature>
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        <location>
            <begin position="379"/>
            <end position="391"/>
        </location>
    </feature>
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        <location>
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            <end position="495"/>
        </location>
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        <location>
            <begin position="511"/>
            <end position="521"/>
        </location>
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        <location>
            <begin position="522"/>
            <end position="533"/>
        </location>
    </feature>
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        <location>
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            <end position="557"/>
        </location>
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        <location>
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        <location>
            <begin position="585"/>
            <end position="595"/>
        </location>
    </feature>
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        <location>
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            <end position="681"/>
        </location>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ81888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="691" mass="74277" checksum="C57F269AC53A41F2" modified="2010-06-15" version="1">MEADELMDDDLFADLYGEDEPPKTSLPAPVKVAKPASPVNTPAAAAPAVVPQRDPEASYSQAQQPQEPAAPQVTAQRAIDPRAPPPSVNVHNASTKIENPYAAEDDADNYSGWDAAAATGGAGAGGHSGQGGHGIHSNDGGSCGYDGMGAAGYGDPQATGSPAIKEDGKMFIGGLNWETTDESLKEYFSQYGEVVECTVMRDGPTGRSRGFGFLTFKDPKTVNIVMVKEHYLDGKIIDPKRAIPRDEQEKTSKIFVGGVSQEATETDFREYFTQFGRVLDATLMMDKDTGRPRGFGFVTFDSEGAVENALNCPTLAILDKPIEVKKAQPRGNIRERMNAHFDERDDRGGSFRDRGDRRDRFENRDNGNNGNGNYNGGSSQNQNAPAGQQNIYNGISPQMMAQYYQNFQRYMQAMQSFGRGGGGPMPAMNPAIMQALGRGGGAMNAMNPAMLQQLQQMQQLQLQQQLQQQGQQQQGQPQQSQMGGAGSQGPQSQQQVGYGSPNQPVGYPHTQGVQQSQQVQSYDPSSYNDQNTGYRHRNFQHHPSQRDYQPPQTQPSSWEGMYDDVPPQQQQQQQQQQQGRGRNEPFQQSWPSNYDNWDDVDQATGVGRAGNGFDGPPPNAPTGPRNAKGSGSNYRGGGRGGVASVRGVAAGSGGVGAGGAGPDRPGFVGGAGGYARGGGGYHPYARNTERY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBV6</accession>
    <name>D5GBV6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00005604001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ81956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837765.1">
        <property type="nucleotide sequence ID" value="XM_002837719.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBV6"/>
    <dbReference type="GeneID" id="9182993"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005604001"/>
    <dbReference type="eggNOG" id="ENOG502S4CM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058475_1_0_1"/>
    <dbReference type="InParanoid" id="D5GBV6"/>
    <dbReference type="OMA" id="WANAIPN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025870">
        <property type="entry name" value="Glyoxalase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40265">
        <property type="entry name" value="BLL2707 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40265:SF1">
        <property type="entry name" value="GLYOXALASE-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13468">
        <property type="entry name" value="Glyoxalase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyoxalase-like" evidence="1">
        <location>
            <begin position="6"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="289" mass="32276" checksum="CB1A92C3BC222B60" modified="2010-06-15" version="1">MAPAQIDHVVILVPYADLHNPPSWITNNFTLTPGGRHSDGKTENKLIIFEDGTYLELVAFIDDNPSLRRGHFWGHKKYGFIDFALTTNPKDTFDYAGLRGRIHQKSGGLGIDYDQPVEGGRKRTQDGKDVRWKVTFPKGEARVGAPRSVSRRGEIPFWCHDITPRNLRVDVTKERTTHLSGVKGIAQFTILVPDGQEAAYLDLYSSITDSSPVQVFGTARLPVDTPVSVPGLVKPWVFVESPTLDIERQRLRDRGPGIVEIALRLGIEGQVGVGRNSIDEKGMWIHFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBX4</accession>
    <name>D5GBX4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00005629001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ81974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837783.1">
        <property type="nucleotide sequence ID" value="XM_002837737.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBX4"/>
    <dbReference type="FunCoup" id="D5GBX4">
        <property type="interactions" value="279"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GBX4"/>
    <dbReference type="GeneID" id="9185409"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005629001"/>
    <dbReference type="eggNOG" id="KOG1287">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013661_4_1_1"/>
    <dbReference type="InParanoid" id="D5GBX4"/>
    <dbReference type="OMA" id="FIVNTEY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050598">
        <property type="entry name" value="AminoAcid_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785:SF382">
        <property type="entry name" value="LOW-AFFINITY METHIONINE PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="109"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="152"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="233"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="320"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="369"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="419"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="448"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="493"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="525"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="586"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="16"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="606"/>
            <end position="616"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ81974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="638" mass="68529" checksum="101B655D614E6CC1" modified="2010-06-15" version="1">MPGHLKPGDRGSVELSSAGGDDGSSSAPGRGAFGDFETDFPTIGATAGAANGQCDDPTVYDVQTFVIPEDRKLGYWSTAALICNQMIGTGIFSTPSTVLRATGTPAGSLLLWCVGAFLAFCGMAVFLELGTALPRSGGEKVYLERIYRKPKYIATCVYAIQFILLGNTAGNAVSCAAHFLRAIDPRRYSVNIQQHGEALPTPPPEGLSKGLAIGVITLNCLIHLYWPKVGGKYLMTAFAALKISLLVLIVIAGIAAGAGRGLDNPQSKNFYGDGAWKRDGATDAPGSWAAALLAVMLPRGWESANYVLGEVRRPAKTLRVSAPIAILGVAALYILANIAYFAALPKEVIQNGGVTVAADFFLDVFGRNAFATTIVPLCIASSAFGNVLAVTFANSRVKQELGKEGVLPWSRFWASDKPFGTPAAALLLHWVFSVVIISAPSSDSEDTYDFFICLFTSSQTWVSLFIGGGLLYLQHSPSQSEIWAAERTDYHVWWPFTLTFCLACVFLLIAPFIPNEYGDGSLLTGSIPFYVFPTVSLCVFVAGFIYWFGFAKIWPKFGFTLEVERAEDELGNEVIKYKHVKRQRETRAEASSTSALPQFRENLGTDEVRMSQRKEVSPGASSGGEDNAHGHSPSAHAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GC19</accession>
    <name>D5GC19_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00005772001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldose epimerase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430098">
        <property type="protein sequence ID" value="CAZ82062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837871.1">
        <property type="nucleotide sequence ID" value="XM_002837825.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GC19"/>
    <dbReference type="FunCoup" id="D5GC19">
        <property type="interactions" value="711"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GC19"/>
    <dbReference type="GeneID" id="9181331"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005772001"/>
    <dbReference type="eggNOG" id="KOG1604">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031753_0_1_1"/>
    <dbReference type="InParanoid" id="D5GC19"/>
    <dbReference type="OMA" id="CVAIEAQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004034">
        <property type="term" value="F:aldose 1-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033499">
        <property type="term" value="P:galactose catabolic process via UDP-galactose, Leloir pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09019">
        <property type="entry name" value="galactose_mutarotase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008183">
        <property type="entry name" value="Aldose_1/G6P_1-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047215">
        <property type="entry name" value="Galactose_mutarotase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10091:SF2">
        <property type="entry name" value="ALDOSE 1-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10091">
        <property type="entry name" value="ALDOSE-1-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01263">
        <property type="entry name" value="Aldose_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072057" evidence="4">
        <location>
            <begin position="17"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ82062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="397" mass="43604" checksum="29290A8C1AA9CCC0" modified="2010-06-15" version="1">MKTLSVLFIIFSAVQGAAIPSPGPDGKYTLTAEGIRAQVIPLRCRQLNRVDRLWQFIPFGAAITNLFVEGADGVERDIVIGFDDVSEYPAHGLGHYGAVPGRYTNRIGNAQYTMDGVTYYLDKIDGNNTLHSGRNGWGYRTFNVAALSENSITFSIHDASNSSMGMPGDVDGYITYTLSQKTWSVKMEATAPQHKTPLMLTQHTYWNLDAFGNPDTDLIWNHSYYMPFSQRLLEPDPSMLPTGRIISIPAGEINDFWSGPRLLGAGSNDPLWKGNCGSESGCEGYNNCWIVDKPTSQATAPVATLSSAWSGIKVDIYTDQAGMQVYTCYWTGSDLPIKKTQGGPGAATDGFMKPSGCVALEPHDWVDGINHPEWGRKQIYGPGETYTWEATYKFSTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GD09</accession>
    <name>D5GD09_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00000895001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430134">
        <property type="protein sequence ID" value="CAZ82402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838211.1">
        <property type="nucleotide sequence ID" value="XM_002838165.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5GD09">
        <property type="interactions" value="46"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GD09"/>
    <dbReference type="GeneID" id="9183868"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000895001"/>
    <dbReference type="eggNOG" id="KOG3628">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000737_0_0_1"/>
    <dbReference type="InParanoid" id="D5GD09"/>
    <dbReference type="OMA" id="LVWTYWT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010506">
        <property type="entry name" value="DMAP1-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056881">
        <property type="entry name" value="Mug62_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22754:SF32">
        <property type="entry name" value="DISCO-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22754">
        <property type="entry name" value="DISCO-INTERACTING PROTEIN 2 DIP2 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23024">
        <property type="entry name" value="AMP-dom_DIP2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06464">
        <property type="entry name" value="DMAP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24919">
        <property type="entry name" value="Mug62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01137">
        <property type="entry name" value="DMAP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51912">
        <property type="entry name" value="DMAP1_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DMAP1-binding" evidence="2">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1589"/>
            <end position="1623"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1665"/>
            <end position="1725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1666"/>
            <end position="1677"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ82402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1725" mass="189925" checksum="34A94F17A2F19748" modified="2010-06-15" version="1">MDDPELQARLAALDSELEEGDITEKGYQKRRTLLFSQYSVDMSDMGPSNRHVYEIRNEPASPFNRRDSGRDSVHSPGSFSTHLESGAMTSATVTQYGGISRESTTVGSTQEWGGIDAVGGGVRTGYAQGQLHVRNGSMSQQSSNPRDSIATSMHVRNPSTASGAASASPLVTYQQPQYDYNSQHPPHIVGPIEYPLNPGYGGPSSPGRESRENTLGGYFSDFTAAGYDHNMMHRYSGEQGVPFSPSVAIPPPMLSSNDLPPPGAVNHMLPLEPRDVPFAVYDPHNSNTPMSKFDNLASVLRHRAGSVPKQPAYIVLDSRGKEIAACSWEKLASRAEKVAQVIRDKSNLYRGDRVALVYRDVEVIEFAVALFGCFIAGVVAVPINNLEDYQKLNHVLTTTHSHLALTTDNNLKVFQRDLTTQKLSWPRGVEWWKTNEFGSWRPGKNQEVPALSVPDLAYIDFARSPEGELRGVVMNHRTIMHQMACLSAIISTVPHDDSKDTFHPGLRDRTGRPVSGRPAGDILVSYLDPRQGIGMIFGALLAVYGGQTTVWLSQATATTPGLYANIITRYKATLCLADYPALKLVAYNYQKEPMVTRNFQKRLTVDFSTVRFMLIDALTHGGMVISMRDWLGGEERMGCPLHQPEEHGDDKKVTELTEVLLDKEALKTNDVVVLAVGEEAQKKAGDPGAVRVGAFGYPIPDATLAVVDPETLLLCAPNVIGEIWVDSPSLSGGFWALTKQTENIFHARPYRFKEGNPHPVSLELEFLRTGLLGCVIEGKIFVLGLYEDRLRQKVEWVEEGAEGREEFRCFFVQHLVCSIMKNIPKIYDCSAFDVYVNEEHLPIILLESQAASTAPTSAGGVARQLDIALLDQLSERCMDVLLDEHHLRVYCVMITAPNTLPRVLKNGRREIGNMLCRNEFHSGTLPCVHVKFGVHRAVLNLPIGIDPNGGIWSHLATQSRQDILLMQEKQYSGVDYREVVIDDRTSTPLNNFSSIVDLLQWRVARQAEELSYCTIDARYREGKGVTWKKLDTKIAAIAVQIKNKWKVKAGDHVVLMYTHSEDFVFAVHACFCLGVIAVPIAPLDQNRLSEDIPALLHMIADFSVQAILVNTEVDHLLKGKPLSTHLKQSAVVLKINMPPIHNTAKPPKSNSGCRDLGLTMKAEWVQSEFIALVWTYWTPDQRRIAVGLGHDTIMGICKVMKETCQMTSSRPVVGCVRSTSGVGFVVSLLMGVFVGAPTYLVSPVDFAQNPLSLFSTLSRYKIKDTYATPQMLDHAISTMPSKGFTMLELKNMMITCEGRPRPDIFQRVRLHFAATELDRTAINTIYSHVLNPMVASRSYTNTEPIELWLDTKALRRGLVYSVDPETHPNALLVQDSGMVPVSTQVAVVNPETCMLCRTGEYGEIWVESEACVKSFYGSKDTFDADRFNGKTIDGDLRITYVRTGDLGFLHTVSRPIGPGGAQVEMQVLFVLGSIGETFEVNGLSHFPMDIEDSVERCHRNIVSGGSAVFQAGGLVVVLVEVRVKSYLASMVPVIVNAILNAHQLVVDIVAFVGKGDFPRSRLGEKQRGKILASWVTRKMRTIAQFGIRDTDRNENPEAGQPPTIVEASHSMTGGDGDEKHGVDLPFELADNQLSGLSPIELPTFYAGFPIEEILGEGGEGTDYHRSMTSSTCMTPTISTPPPKSARRQPISYQQNLYAVHPSDTAGGGGGVDSAAAPENQSPLGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDI1</accession>
    <name>D5GDI1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00001031001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430144">
        <property type="protein sequence ID" value="CAZ82574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838383.1">
        <property type="nucleotide sequence ID" value="XM_002838337.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDI1"/>
    <dbReference type="FunCoup" id="D5GDI1">
        <property type="interactions" value="49"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GDI1"/>
    <dbReference type="GeneID" id="9188164"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001031001"/>
    <dbReference type="eggNOG" id="KOG4533">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048479_0_0_1"/>
    <dbReference type="InParanoid" id="D5GDI1"/>
    <dbReference type="OMA" id="YPENLPW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055323">
        <property type="entry name" value="C57A10.07/YOR238W"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28110">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28110:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ82574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="216" mass="24346" checksum="01200FF7ACF870EE" modified="2010-06-15" version="1">MIEDFQKGEVDTYVRHIKAGVEAVSEDAESLLVFSGGETKSAAGPLSEAESYYRLAHTLDLLTPSLLPRTTTEVHARDSYQNLVFSIARFHELTNTYPSSIALISHAFKESRFLHNHLRAISYPEANFKFVGIDPKEGVGDLVLKGETRTRQVWDEDLYGCKSDGELRRKRQGRNPGRRWDGGYSVTCPELRELLTYCPSDGVSEFGRELPWSKST</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDU2</accession>
    <name>D5GDU2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00006275001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430153">
        <property type="protein sequence ID" value="CAZ82685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838494.1">
        <property type="nucleotide sequence ID" value="XM_002838448.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDU2"/>
    <dbReference type="STRING" id="656061.D5GDU2"/>
    <dbReference type="GeneID" id="9184480"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006275001"/>
    <dbReference type="eggNOG" id="KOG2632">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084816_0_0_1"/>
    <dbReference type="InParanoid" id="D5GDU2"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S54 rhomboid" evidence="7">
        <location>
            <begin position="4"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="147"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="147"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ82685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="179" mass="19194" checksum="17DCC058AE354510" modified="2010-06-15" version="1">MVGVALVTFPGGLYVGIEILLLGAYDSVAGASALVFTLMANEAVKTFLEKPTYSIAGYGIPSWMAPIFWLVVVSILVPGASILGHFCGLIIGYLYACHYLRLLEPSENILGKVESKLNFITRRVPFYISLENRTEMNHMELLPTSISSRTQSTSRATTSIPMESGSGFAAAGPGRVLGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDU8</accession>
    <name>D5GDU8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00006281001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430153">
        <property type="protein sequence ID" value="CAZ82691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838500.1">
        <property type="nucleotide sequence ID" value="XM_002838454.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDU8"/>
    <dbReference type="FunCoup" id="D5GDU8">
        <property type="interactions" value="922"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GDU8"/>
    <dbReference type="GeneID" id="9184486"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006281001"/>
    <dbReference type="eggNOG" id="KOG0733">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_8_3_1"/>
    <dbReference type="InParanoid" id="D5GDU8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990275">
        <property type="term" value="F:preribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000018">
        <property type="entry name" value="Cell division control 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000365">
        <property type="entry name" value="Ribosome biogenesis ATPase RIX7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF171">
        <property type="entry name" value="NUCLEAR VALOSIN-CONTAINING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="82"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="360"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="584"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="36"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ82691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="609" mass="67009" checksum="A0E4A87545F1DB6C" modified="2010-06-15" version="1">MNEQVAGNAVTGLRVPRKRKEHLPVQGGESVKRQKQNNSHKPPNNISLSDIGVREDVIERLLEFVFMPLIHPEFYLHMGVDLPRGVLLHGPPGCGKNMLANAISRELGLPFIAISAPSIVSRMYGGSEKMIREIFEDAREIAPCLIFIDEIDAIAQKRDNTEGDMEKRIVAQMLTCMDDLTLEKTGGEPVMIIGATNRPDSLDPALRRGGRFDKEIYLGVPDEVEREKILRVLCEKLRLTGDFDFKKLARATAGFVGVDLSALAREDGMVAMRRIYETLETPSASTDPLEQLDPLYITFPDFLTALTEIQPSTKREGFTTVPDVTWADVGALKSHKAELQMAIVLPIKNPEVFASVGLTAPSGVLLWGPPGCGKTLLAKAVANESGANFISIQGPELFSKYVGESEQAVRQVFSRARASIPCVIFFDELDALAPPRDNSFSECSPRVVDTLLTELDGFNGSKGIYIIAATNRPDMIDQSMLRPGRLDTLLFVDLPNAEGRVEILKNITKNTPLSNLSNFDLIAIAKHKCKNFSGADLAALVKRATTLALRQSCFTEAGKVKEGKNASNLQVVVTKEHLEKVSAKIRPSVSKDSREQYQEIATRFGPEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEB1</accession>
    <name>D5GEB1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00001244001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430172">
        <property type="protein sequence ID" value="CAZ82854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838663.1">
        <property type="nucleotide sequence ID" value="XM_002838617.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEB1"/>
    <dbReference type="STRING" id="656061.D5GEB1"/>
    <dbReference type="GeneID" id="9185511"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001244001"/>
    <dbReference type="eggNOG" id="ENOG502QW5G">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024934_6_0_1"/>
    <dbReference type="InParanoid" id="D5GEB1"/>
    <dbReference type="OMA" id="WYITIRA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053157">
        <property type="entry name" value="Sterol_Uptake_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47784">
        <property type="entry name" value="STEROL UPTAKE CONTROL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47784:SF5">
        <property type="entry name" value="STEROL UPTAKE CONTROL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="2">
        <location>
            <begin position="13"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ82854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="476" mass="52684" checksum="505BE89A85F3129A" modified="2010-06-15" version="1">MPPKRRHTKSRNGCMTCRRRRVKCDEIRPICTNCTRRELDCIYTAYCPPGRPGISRTHSSACASSSCTSSSCTSSSSCTSSLLCTSSSSSSSSLPSSQVVSSRLRILPLQQSHIPALSKPRRGGGGTSISAPTPLAALTRQLDSILIGTRSGTPSPLARISLSKKDLDNLSLLHHFTIRTYSSLTNDKSVEVYRTEIPRIALQNELTMNALLAVTALHMSITVEEPVIVSHYQMIAAGHYDQALRSLRAEIRERPKSGDHILAASTLIAFYGFACKMTSGEASQVPRVIGWIPLFDGVHAIIKEWWPLVIQGDLKPLLDQAITRTVEDGDFLTLPPSLTALVLEALPTSNVAHGDSDEVIIDAATAAVYQRALKHLKIVWNNFWATGYKLATAWQFISHAPAKYTHNLQQYKPRALVIFCYFLCMVKKLDGFWWAKGSARETFLDIEKKLDARWEPWLEWPRNIILGDCEVVGSQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEB8</accession>
    <name>D5GEB8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00001257001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430173">
        <property type="protein sequence ID" value="CAZ82861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838670.1">
        <property type="nucleotide sequence ID" value="XM_002838624.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEB8"/>
    <dbReference type="STRING" id="656061.D5GEB8"/>
    <dbReference type="GeneID" id="9185516"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001257001"/>
    <dbReference type="eggNOG" id="ENOG502S3NK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054608_0_1_1"/>
    <dbReference type="InParanoid" id="D5GEB8"/>
    <dbReference type="OMA" id="WCERWGE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SET" evidence="2">
        <location>
            <begin position="70"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="11"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ82861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="217" mass="23955" checksum="55E99B6CB14BAA56" modified="2010-06-15" version="1">MPTKGPPRAPKPLSKTTITSSSSSKLSKTPSNWPPEIIYLTRPRLSRSLPAEALTHLSLSPTSPPSIPSTLVRIRKITDLAHPAFGQLGLFAAKTLPARSFILDYLGYVHDDNDLDESSDYDLCLDKELHVGVDARSMGNEGRMVNDYRGVPGLVRANVVFETRRIGGDNEGEVRMGLWVGSRDVKKGVELCVSYGKGFWKERVGEVEDLSLNYSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEI0</accession>
    <name>D5GEI0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00006491001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the alpha-actinin family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430175">
        <property type="protein sequence ID" value="CAZ82923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838732.1">
        <property type="nucleotide sequence ID" value="XM_002838686.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEI0"/>
    <dbReference type="STRING" id="656061.D5GEI0"/>
    <dbReference type="GeneID" id="9181554"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006491001"/>
    <dbReference type="eggNOG" id="KOG0035">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005217_0_2_1"/>
    <dbReference type="InParanoid" id="D5GEI0"/>
    <dbReference type="OMA" id="QQRWITV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032432">
        <property type="term" value="C:actin filament bundle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110085">
        <property type="term" value="C:mitotic actomyosin contractile ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051764">
        <property type="term" value="P:actin crosslink formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051017">
        <property type="term" value="P:actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071520">
        <property type="term" value="P:actomyosin contractile ring assembly actin filament bundle convergence"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd21215">
        <property type="entry name" value="CH_SpAIN1-like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000138">
        <property type="entry name" value="Alpha-actinin, sarcomeric"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000097">
        <property type="entry name" value="Alpha-actinin, sarcomeric (F-actin cross linking protein)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000223">
        <property type="entry name" value="Related to alpha-actinin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000030">
        <property type="entry name" value="Related to alpha-actinin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000077">
        <property type="entry name" value="Related to alpha-actinin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001589">
        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014837">
        <property type="entry name" value="EF-hand_Ca_insen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11915">
        <property type="entry name" value="SPECTRIN/FILAMIN RELATED CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08726">
        <property type="entry name" value="EFhand_Ca_insen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01184">
        <property type="entry name" value="efhand_Ca_insen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00019">
        <property type="entry name" value="ACTININ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00020">
        <property type="entry name" value="ACTININ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="5">
        <location>
            <begin position="8"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="5">
        <location>
            <begin position="123"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="483"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ82923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="629" mass="72378" checksum="8C6709C91F39E929" modified="2010-06-15" version="1">MAQQQWITTQDKTFRKWLNTKLSARNIPPAQSLQKDLATGVLLIHLLEILGNESLGKYASNPRLRVQKCENVHKALEYIRGRGIQLFNIGAEDIVDGNLKLILGLLWTLILRFTIADINAEGLTAKEGLLLWCKRKTACYEEIDITDFSTSWNNGLAFCALLDFHRPDLIDYDALDKSDHKGNTALAFKIATEEIGIPALLDVEDVCDVAKPDERSLVTYIAYWFHAFSQLERIETAGRRVEKFVEVMQGAWEMQHGYEKRMAELLRQMHEMKQQWSTATFVGTYFDAKQQSQKFSSYKRTLKRAWVAEKADLVSLLGNIKTKLSTYRLKDYEPPAHLSLANLDLEWKKLLQDENKRSIVINEKIRDIKEGLRRSFADAANEFALCLNTVSLQISELDGDPEHQLRIVRTLHRNIIPLELELNRIEKLSDDCEEANIEENDYTVYSYDELEYEYGLAKESVAKKLSFLDNQIVARSMTNLTPIQLEEFESVFRHFDRDLSNSLQEIEFSAALASLGLVYDEEEMHEVFKEVSARGRSVTFEQFIRFMVEVTEDQNSAEQVYQSFKEVADGKPYVTEFDLRHSLVPDGMIEELVSTMPKVRAEDGEVGFDYEKYMEGLMGKKLDTNGVHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEQ5</accession>
    <name>D5GEQ5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00001330001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="FN430184">
        <property type="protein sequence ID" value="CAZ82998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838807.1">
        <property type="nucleotide sequence ID" value="XM_002838761.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEQ5"/>
    <dbReference type="FunCoup" id="D5GEQ5">
        <property type="interactions" value="343"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GEQ5"/>
    <dbReference type="GeneID" id="9184911"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001330001"/>
    <dbReference type="eggNOG" id="KOG1152">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004134_0_0_1"/>
    <dbReference type="InParanoid" id="D5GEQ5"/>
    <dbReference type="OMA" id="MDESECR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045719">
        <property type="term" value="P:negative regulation of glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14004">
        <property type="entry name" value="STKc_PASK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000320">
        <property type="entry name" value="Serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000314">
        <property type="entry name" value="Serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057213">
        <property type="entry name" value="DUF7891"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF51">
        <property type="entry name" value="PAS DOMAIN-CONTAINING SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25421">
        <property type="entry name" value="DUF7891"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="861"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="216"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="774"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="823"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="33"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="281"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="774"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="788"/>
            <end position="799"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ82998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1142" mass="125496" checksum="FEB005097D66D1C3" modified="2010-06-15" version="1">MAGLTTPDRNVSEISREGLDMMEGLVRPRSTPPIKSQSLHLSTNTGGSSYKPKIRFSVDSSMDPQQHSSGEQVTQSPVTVGESDHLYTSGLRRIRQQTPLAGTQSFVIPHSMSVPGTRVNSLSIPPTSPTLGFCEDLARFPTESLHSFSFAHQSEDLIHSRQNILKRSIEFMRDKLGWGSQSPMITSAQAKVEGDEEVAKVMELLRKANLVSEEKTPLMNGPVTGPAQFEGNPFEKSFAPEAPVSPRTQATNSALPLRGTDRAGSFNDEFRSESTSRSTTNDSISTAHSSPPSTRRASLKRTYTDTTSLTVQTKLYEALAQPYLVTDMPQSATGMGPRPIMSKSSVHGHTSRYTLASQAVFTTEAHHPWTILAANDLACLVFGVTKAEVRKIGILEVVKEERREWLANKLNSGENSSSSFPYPHSHHGSPNVTSAMLQNGPYGNGYRRVKSDAYYTGSPIRSGGCSAGVAQPSMHHSQNKSRGVILCGDVVPIQKRNGNTGAASLWVKEKRGGLIWVVEEISEDVAFLYTSNDPDLAGKVMSFSGNMTKIFGEDSIPDGGVDVSDFIPMIPRNGDGTIDLEQTAGIHQFTAMNSIGMRIPCGVEPIPCEGVLRISSFPHIAGIIVLSATTLRITSSNAVFSAALFGHLNPVGVPITEIIPQFDRILKFITSEDKIDLVDGIVVPEHSFRKAKAVLALRDGKGDAASMFVRSGGLTARHRDGAELRVDVQMRVVMSESKDEEAVIIDDPDETGAGGGEVVYALWITYSRHMHASSLTSDPSNNILRRPVTPPKRPSPGQPSTPIASDGQRSDNAKLLTRQLEAIQQSPTPPSSEDESTEPSGHVSASGPQGMSKRKKNIQDFVILEEMGQGAYGQVKLARYKRNQAKRVVLKYVTKRRILVDTWTRDRRLGTVPLEIHVLDYLRRDGLKHPNIVEMTDFFEDDINYYIEMVPHGLPGMDLFDYIELKVNMDEDECRSIFVQVARALHHLHTKAGVVHRDIKDENVVLDGEGNIKVIDFGSAAYIRNGPFDVFVGTIDYAAPEVLAGKSYKGKEQDVWALGVLLYTICYKENPFYNIDEIMDRELRIPFILSEVSLELIRLMLDRDVERRPTIEQVLAHPWFDGLPSPEGALLAESAVQASFPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEZ0</accession>
    <name>D5GEZ0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00006666001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCDC124 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430197">
        <property type="protein sequence ID" value="CAZ83083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002838846.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEZ0"/>
    <dbReference type="STRING" id="656061.D5GEZ0"/>
    <dbReference type="GeneID" id="9188621"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006666001"/>
    <dbReference type="eggNOG" id="KOG3223">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069723_1_1_1"/>
    <dbReference type="InParanoid" id="D5GEZ0"/>
    <dbReference type="OMA" id="FEERMMP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010422">
        <property type="entry name" value="Ccdc124/Oxs1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054414">
        <property type="entry name" value="Ccdc124/Oxs1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054413">
        <property type="entry name" value="LSO1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21680">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 124"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21680:SF0">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 124"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06244">
        <property type="entry name" value="Ccdc124"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22048">
        <property type="entry name" value="LSO1_2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LSO1/LSO2" evidence="5">
        <location>
            <begin position="9"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Coiled-coil" evidence="4">
        <location>
            <begin position="102"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="11"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="26"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF06244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF22048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ83083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="211" mass="22911" checksum="78FE32963CEBD9A5" modified="2010-06-15" version="1">MPKKQVENSKKAAGNAKKAAAAATKADAENSRRAAEEAQEWEKGAKSNAKKEAAEAKKRDAAAKKAAAAAALAEEERALPSKPKPARAGPAKRTKGIDSALGSLNASNVDDALDALTLATGAEKGTIDRHPERRYKAAYAAYEERRLPEFREEHKGLRLGQMKELIKKEFEKSEENPFNQVGNVRYDATKDEIALAKKAEMEKIEVRLGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFD8</accession>
    <name>D5GFD8_TUBMM</name>
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        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00006845001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430208">
        <property type="protein sequence ID" value="CAZ83231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839040.1">
        <property type="nucleotide sequence ID" value="XM_002838994.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFD8"/>
    <dbReference type="STRING" id="656061.D5GFD8"/>
    <dbReference type="GeneID" id="9188179"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006845001"/>
    <dbReference type="eggNOG" id="ENOG502S73X">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065618_1_0_1"/>
    <dbReference type="InParanoid" id="D5GFD8"/>
    <dbReference type="OMA" id="NTGTYGW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018466">
        <property type="entry name" value="Kre9/Knh1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052982">
        <property type="entry name" value="SRP1/TIP1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40633:SF1">
        <property type="entry name" value="GPI ANCHORED SERINE-THREONINE RICH PROTEIN (AFU_ORTHOLOGUE AFUA_1G03630)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40633">
        <property type="entry name" value="MATRIX PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_8G05410)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10342">
        <property type="entry name" value="Kre9_KNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072068" evidence="3">
        <location>
            <begin position="21"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="Yeast cell wall synthesis Kre9/Knh1-like N-terminal" evidence="4">
        <location>
            <begin position="27"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="115"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="115"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="159"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="193"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ83231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="245" mass="24291" checksum="B651F4EFEFE44562" modified="2010-06-15" version="1">MRFLTSSLLAIAALATSVFAAENPIIKPDGSAPLAAGDTVTITWTPTTSGPITLKFRQGPSNDLKDVTTIVAGADNSGSYSWTVPKDTPSGANYAIQIGDDKTTDVNYTPLLTVSSDAPSSVSSSSTASSSHASSSTSSSASSITTTSKPATTSKPAHHNSTMVTSTTSASNGTISVHTASSNGTATRSTVESGGKPTLPGSSGPSSTGSSPPASTAPSSGAVSLVMNSPLALVVCVLGAVMYLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFJ1</accession>
    <name>D5GFJ1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00006929001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S33 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430208">
        <property type="protein sequence ID" value="CAZ83284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839093.1">
        <property type="nucleotide sequence ID" value="XM_002839047.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFJ1"/>
    <dbReference type="STRING" id="656061.D5GFJ1"/>
    <dbReference type="ESTHER" id="tubmm-d5gfj1">
        <property type="family name" value="Proline_iminopeptidase"/>
    </dbReference>
    <dbReference type="GeneID" id="9188232"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006929001"/>
    <dbReference type="eggNOG" id="ENOG502QSNW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024518_2_1_1"/>
    <dbReference type="InParanoid" id="D5GFJ1"/>
    <dbReference type="OMA" id="TNEYEHN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002410">
        <property type="entry name" value="Peptidase_S33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051601">
        <property type="entry name" value="Serine_prot/Carboxylest_S33"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43248">
        <property type="entry name" value="2-SUCCINYL-6-HYDROXY-2,4-CYCLOHEXADIENE-1-CARBOXYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43248:SF2">
        <property type="entry name" value="PROLYL AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00793">
        <property type="entry name" value="PROAMNOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="66"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ83284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="460" mass="52145" checksum="FF9FC9AB54BECAED" modified="2010-06-15" version="1">MQKIPPATLLECEEYTITGGIKIVDRWFRTALDYSNPGAGEITLFARNALPNKKGNAECGAALPYFLYLQGGPGFECGPPENHPLTNFLFDKGFQVLYLDQRGTGMSTPISPRMLEEDRGGKKRSAKEQAEYVKLFRADNIVRDCEAVRHALLGRKEEEEDRKWGVVGQSFGGFCAVTYLSFYPEGLREVFTTGGMPPLVNDPDEVYKALWEKVRLRNRVYYKKYPLDIERVKDILRHLAKEDVRVPNGGRLTSRRFLGLGLAFGGHGGIDGVHHLVQRAHTDLKVLGHLSYKVLQSIQGNQSFDGNVLYALVHEPIYCQHRAAEWSALRTKPNDPEFNLAQSLERKGQPAYFTGEMIFPWTFEDHAELSKLHYVAKTLAEYTEWPSLYCEDVLAENRVPVYAATYIDDMYVDCTHARRTAGKIRGAKEFVTNALMHNAIRARTETVMGELWRLRSGEVD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-01-25" modified="2025-06-18" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFR0</accession>
    <name>MTAP_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">S-methyl-5'-thioadenosine phosphorylase</fullName>
            <ecNumber evidence="1">2.4.2.28</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">5'-methylthioadenosine phosphorylase</fullName>
            <shortName evidence="1">MTA phosphorylase</shortName>
            <shortName evidence="1">MTAP</shortName>
            <shortName evidence="1">MTAPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ORF">GSTUM_00007013001</name>
    </gene>
    <organism>
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-methyl-5'-thioadenosine + phosphate = 5-(methylsulfanyl)-alpha-D-ribose 1-phosphate + adenine</text>
            <dbReference type="Rhea" id="RHEA:11852"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58533"/>
            <dbReference type="EC" id="2.4.2.28"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="CAZ83353" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.28" evidence="1"/>
    <dbReference type="EMBL" id="FN430220">
        <property type="protein sequence ID" value="CAZ83353.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFR0"/>
    <dbReference type="SMR" id="D5GFR0"/>
    <dbReference type="FunCoup" id="D5GFR0">
        <property type="interactions" value="385"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GFR0"/>
    <dbReference type="eggNOG" id="KOG3985">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="D5GFR0"/>
    <dbReference type="UniPathway" id="UPA00904">
        <property type="reaction ID" value="UER00873"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017061">
        <property type="term" value="F:S-methyl-5-thioadenosine phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09010">
        <property type="entry name" value="MTAP_SsMTAPII_like_MTIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1580:FF:000008">
        <property type="entry name" value="S-methyl-5'-thioadenosine phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="entry name" value="Nucleoside phosphorylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01963">
        <property type="entry name" value="MTAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010044">
        <property type="entry name" value="MTAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018099">
        <property type="entry name" value="Purine_phosphorylase-2_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01694">
        <property type="entry name" value="MTAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42679">
        <property type="entry name" value="S-METHYL-5'-THIOADENOSINE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42679:SF2">
        <property type="entry name" value="S-METHYL-5'-THIOADENOSINE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="Purine and uridine phosphorylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01240">
        <property type="entry name" value="PNP_MTAP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0539">Nucleus</keyword>
    <keyword id="KW-0660">Purine salvage</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000415136" description="S-methyl-5'-thioadenosine phosphorylase">
        <location>
            <begin position="1"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="64"/>
            <end position="65"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="97"/>
            <end position="98"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="225"/>
            <end position="227"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="1">
        <location>
            <position position="183"/>
        </location>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="1">
        <location>
            <position position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="309" mass="33148" checksum="0D3FA92C97BF9093" modified="2012-01-25" version="2">MPLPDTFSESVRIAVIGGTGISALEAEGFTAVASLDIQTPYGAPGSPLTILKTPQNTPIAFLSRHGLHHELTPTEIPNRANIAALRSIGVRSIIAFSAVGSLQEHIRPRDFVVPSQIIDRTKGLRASTFFEGGLVGHVGFADPFDSELSKVVFDIGSDGVLNGEGVKMHKDALLICMEGPQFSTRAESNLYRSWGGDVINMSALPEAKLAKEAEIAYVMVCMSTDYDCWKTNEAAVTVETVMGNMHANGANAKHLAAAILKELAKEEHQDLVGAKHLEGLSKWACCTAPAGRKAEVVKKIEFMLPGYFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGG5</accession>
    <name>D5GGG5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00007375001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the velvet family. VeA subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430264">
        <property type="protein sequence ID" value="CAZ83608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839417.1">
        <property type="nucleotide sequence ID" value="XM_002839371.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGG5"/>
    <dbReference type="STRING" id="656061.D5GGG5"/>
    <dbReference type="GeneID" id="9183206"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007375001"/>
    <dbReference type="eggNOG" id="ENOG502QVY9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022491_2_0_1"/>
    <dbReference type="InParanoid" id="D5GGG5"/>
    <dbReference type="OMA" id="NHFVTHR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034250">
        <property type="term" value="P:positive regulation of amide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051176">
        <property type="term" value="P:positive regulation of sulfur metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043455">
        <property type="term" value="P:regulation of secondary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.3960:FF:000001">
        <property type="entry name" value="Sexual development activator VeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3960">
        <property type="entry name" value="Velvet domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021740">
        <property type="entry name" value="Velvet"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037525">
        <property type="entry name" value="Velvet_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038491">
        <property type="entry name" value="Velvet_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33572:SF14">
        <property type="entry name" value="DEVELOPMENTAL AND SECONDARY METABOLISM REGULATOR VEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33572">
        <property type="entry name" value="SPORE DEVELOPMENT REGULATOR VOSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11754">
        <property type="entry name" value="Velvet"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51821">
        <property type="entry name" value="VELVET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Velvet" evidence="9">
        <location>
            <begin position="26"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="219"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="226"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="261"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="345"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="376"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="409"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="431"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="472"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="490"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="535"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="566"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="602"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="641"/>
            <end position="652"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ83608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="696" mass="77565" checksum="36520227C800FAF0" modified="2010-06-15" version="1">MSGPKTISIHDVSETVATVSRTLQNGTKLEYRMQVLQQPERARACGQGAKSHADRRPVDPPPVVELRLFEGHEKTDVTFSHNSNFFLFATLEQARPIAQGRGMPAPAPAPVLTGVPVSGMAYLDRPNPAGYFIFPDLSVRHEGKYRLRFALYEETKEEEGYPAPQDPLEPREYCTHRLDVKSRAFTVFSAKKFPGLAENTALSRIVAEQGCRVRIRRDVRMRRRSDRPSRDSEDRHMDETAMLRASRRAATPDQYEIENSIRAQHTPQYHQSTPHPMQRSNSINRKRSGSDASMISAMGDMPQAPRHENGYSTPNQNYPPYGPPAGPPSQSAHQHLSFGSNGHAPAPPAPPAPHVHPPQELYGQDMQSRYPPPPSHAQNNVHHPSPHMPSSTRTQQHPYGAPQYHSRENSISLPNQQVQQQPRHEPHHVRRDSGEFRARRDSDPAYRQQPPQHYRPGTPVASPYGQMENGYGHQQQQQHQPYQPGPPAPQQYHHQQPQQQPQQHAPPPPQSTGNSLPPIRLPALGLPALEPKIWNSQPTTPNSTNPPAALPSPGAYPPHTNAPMPSQRPPPPQMSQPPPPPNPNQFPAYYPEPKYTTPPAPSNDSWGSSNGSAPAKESEGRGTKRPWGRVFNNAHVEGPMQNRSRPSSNSAAYGSDPPPYAQQPEVDDDAYDFGKLKMSYRRADGVEIVHRLPGED</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGK8</accession>
    <name>D5GGK8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00007541001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430275">
        <property type="protein sequence ID" value="CAZ83738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839547.1">
        <property type="nucleotide sequence ID" value="XM_002839501.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGK8"/>
    <dbReference type="STRING" id="656061.D5GGK8"/>
    <dbReference type="GeneID" id="9181708"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007541001"/>
    <dbReference type="eggNOG" id="KOG1041">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004544_4_3_1"/>
    <dbReference type="InParanoid" id="D5GGK8"/>
    <dbReference type="OMA" id="CFAQQQH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033167">
        <property type="term" value="C:ARC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990342">
        <property type="term" value="C:heterochromatin island"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031934">
        <property type="term" value="C:mating-type region heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005721">
        <property type="term" value="C:pericentric heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030958">
        <property type="term" value="C:RITS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140720">
        <property type="term" value="C:subtelomeric heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070551">
        <property type="term" value="F:endoribonuclease activity, cleaving siRNA-paired mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035197">
        <property type="term" value="F:siRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033562">
        <property type="term" value="P:co-transcriptional gene silencing by RNA interference machinery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070317">
        <property type="term" value="P:negative regulation of G0 to G1 transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990431">
        <property type="term" value="P:priRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030466">
        <property type="term" value="P:silent mating-type cassette heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990432">
        <property type="term" value="P:siRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140727">
        <property type="term" value="P:siRNA-mediated pericentric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02846">
        <property type="entry name" value="PAZ_argonaute_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04657">
        <property type="entry name" value="Piwi_ago-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.260.10">
        <property type="entry name" value="paz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014811">
        <property type="entry name" value="ArgoL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032472">
        <property type="entry name" value="ArgoL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032473">
        <property type="entry name" value="Argonaute_Mid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032474">
        <property type="entry name" value="Argonaute_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003100">
        <property type="entry name" value="PAZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036085">
        <property type="entry name" value="PAZ_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003165">
        <property type="entry name" value="Piwi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045246">
        <property type="entry name" value="Piwi_ago-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22891">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08699">
        <property type="entry name" value="ArgoL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16488">
        <property type="entry name" value="ArgoL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16487">
        <property type="entry name" value="ArgoMid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16486">
        <property type="entry name" value="ArgoN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02170">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02171">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01163">
        <property type="entry name" value="DUF1785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00950">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101690">
        <property type="entry name" value="PAZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50821">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50822">
        <property type="entry name" value="PIWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PAZ" evidence="2">
        <location>
            <begin position="224"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="Piwi" evidence="3">
        <location>
            <begin position="528"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="831"/>
            <end position="850"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ83738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="868" mass="98033" checksum="A432B18E6F1369E6" modified="2010-06-15" version="1">MATHTEGDLPKRPAFGTKGRPATIALNSYAVSRFPTLAINQYDVTIGSGNEKRALIKKIWFSDKLQSEFGAAKNTVLFDGNKLAWSVTSFPFGDRHVTSVDLDEGDGRPARRGGGPARDNKHRVVIQKSGRVPLQVVEAFVKGDYRLDNDVLVGINFLDHLMRETPSKHFITIKRSFFQSAGKTPMERGVEAWKGIFQSVRATQGGRLTINVDVATTVFWSQGTVLDIACRMTWNGSPEDLQSKIVKEHGKGVISRDLRRLRRVSFFCTHRKRDTEVRKKVYTIEGFDQNAIDYTFEMKRHNADGGVTTETITAYDYFLRQYNLRLKYPQLPLVKTRKKGEVFPMELCHIQEGQRYPFKLDDRQTAEMIKFTVQRPTIRMEQIKSNVAQLDWKKDPILTKYGMEIDTNMIKSKGRILNAPKICYGDGSTDRVFTPRDGKWDLRGKKFAKIGAPLKGWGFMIFAPQRNCDELTVKTFIRQLVSVYIGHGGQVQNKEPIIMYADPKKSVGTNIFELYKKAGNQVQAKPQMLFFVLSAKSPQPYNEIKAFCELNIGVVSQCVQSRHVAQAKAQYCSNVCMKVNAKLGGTTCFLDKSDHPLFGKEASIIVGADVSHPAPGITKASFASMVGSTDMQGSRFAAICNTNGQGKECITTNNMVKFMCTLLRAFRQETTKIPMRIFYFRDGVSEGQYKQIIDDELRDMREACKVLQADYNPKITVTICSKRHHTRFFPVERNAQDRNGNCLPGTIVERDVTHPTEYDFYLAAHNAIQGTARPVHYHVIHDENKMPVDMFQALVYNSCYTYIRASNSVSLIPAVYYAHLASSRARAHEVADEGNTITTTTSGEKRDPGDVADIRPLHDTIKHAMWYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGT3</accession>
    <name>D5GGT3_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SAGA complex subunit Spt7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00007503001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430275">
        <property type="protein sequence ID" value="CAZ83705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839514.1">
        <property type="nucleotide sequence ID" value="XM_002839468.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGT3"/>
    <dbReference type="FunCoup" id="D5GGT3">
        <property type="interactions" value="405"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GGT3"/>
    <dbReference type="GeneID" id="9181675"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007503001"/>
    <dbReference type="eggNOG" id="KOG1472">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006198_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGT3"/>
    <dbReference type="OMA" id="YRRSEAK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046695">
        <property type="term" value="C:SLIK (SAGA-like) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05510">
        <property type="entry name" value="Bromo_SPT7_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22927">
        <property type="entry name" value="HFD_SPT7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000072">
        <property type="entry name" value="Transcriptional activator spt7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000032">
        <property type="entry name" value="Transcriptional activator spt7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006565">
        <property type="entry name" value="BTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037782">
        <property type="entry name" value="Spt7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47343">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR SPT7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47343:SF1">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR SPT7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07524">
        <property type="entry name" value="Bromo_TP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 8" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Bromo" evidence="10">
        <location>
            <begin position="299"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="96"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="408"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="558"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="940"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="96"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="116"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="142"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="408"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="423"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="570"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="987"/>
            <end position="1001"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00093633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ83705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1035" mass="114938" checksum="81C264DA7B9134B6" modified="2010-06-15" version="1">MSNNNLLPASWSRQPSTRDDVTVLAERTRTNSIETGVEGGDKDDVVDPTIAAFRQRYDIFEKRIAMMFDTRRPCCAPPDMEFAALELNDDFSSANVNASGNSAADNARPPVRKIDDDDYDFEDDEDEEDTKAKAPPFLPLKSASSLSQSSEPSSGNIIDAKKSAVATREKLEADRKAAEEAVKRALPSMFFTLENDRDAMLEQQKLEESDRQVNAEANAAQANALGKLSSANLGASSLTLKHLIARIDAKREKVVVSDMELRNLMSEVRKNRSKWANEDKIGQEDLYEAAEKVVLELRALTEHSTAFLNKVNKRDAPDYTTIIKHPMDLGTVMKKLKGHHYKSKNDFVDDLNLIWQNCLTYNAEPSHYLRKHAKAMQKATQNLIPLIPDIVIRDRAEVELEEAGALEVDAEGESDDEPIMSTRGRKAPGTKKTRKGARLEGTPEVKAPVVNLLRADSNPPGNDSQVSVGINGFPTPSGSTPIPNGLVHSISQMDIDHDRVDDDTHDVEYQTWKTLTKKARAKVASERHKLFRGNRLNPEEPALLRSRDDMGRFLRNEEKHAKSTQDADDSSMQQGSGNTKLVDTEQPDVQELLPDYYDPLAAVPELPSGVSWYGEGDEVDLQDDNLRIAPRGIFTQPKSELSKRMDANIKQMQETRKLCSKIGVIKQMQVQTQMYSNQFTKYDPKEFVEADVEDFVISEEGPIMCATVARAALQKSVGKIFYHAGFEEFQPTAIDAVTDIAVDYFQKLSKTLTVYQEAPQHERKFEPEFLKMLTWLQEMLLHALYENGADVESLDTYIKDDIERLGSKLNGIHQRMKIHLTELLRPALQDVGGDGSRMFNDGSEQFVGGDFAEDIGDDFFGFKELGLDKEFGLSSLSVPLHLLQGRMHSTYQTQNTSAVALADGYKPPPPFEPVTHELLEREIGLVKNFFLAKLHAGGGEPLVEDEDLPVKQRLPKPRLPPSGKILAPRKRPMHGQGGNSKKKKKPNPPAPPPPKAAPPPSKSMAMDRSLSKISEVDGDGNGGMMSPESLVGGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGZ8</accession>
    <name>D5GGZ8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00007634001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430297">
        <property type="protein sequence ID" value="CAZ83791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839600.1">
        <property type="nucleotide sequence ID" value="XM_002839554.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGZ8"/>
    <dbReference type="GeneID" id="9185045"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007634001"/>
    <dbReference type="eggNOG" id="ENOG502QR2T">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010834_0_1_1"/>
    <dbReference type="InParanoid" id="D5GGZ8"/>
    <dbReference type="OMA" id="KLLLNWM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058934">
        <property type="entry name" value="YMC020W-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058933">
        <property type="entry name" value="YMC020W-like_ab_hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47349:SF1">
        <property type="entry name" value="AER328WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47349">
        <property type="entry name" value="CHROMOSOME 8, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26147">
        <property type="entry name" value="AB_HYDROLASE_YMC0-YMC35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YMC020W-like alpha/beta hydrolase" evidence="2">
        <location>
            <begin position="542"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="258"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="349"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="509"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="184"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="288"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="355"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="398"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ83791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="935" mass="100516" checksum="06B546F710519668" modified="2010-06-15" version="1">MAKGEKKDLPPSPKLAASPPDPPSPATGISTVHASGVPSSLPPSESNLRSTSQVSLVSTDVTPPPPTQSFSSMEPKMTGGCVPRKSSYAESLKVSSKGWFSRTGTWSRSGGTKSAAIRKVASENVSREPSIAGGPNKTEGTSGLVPDVGPLEPLPSTTGQASELPRESTPPPSLEEPLAPPAIPTSLSTTTLPTAEPFEAQPEARLPLSMEPLNSASDSGDTKGSLDPGPGNESSERPASYWFGWWYGRQAIVEQPVPKSEAIGEGPEESPVVEIEDPAKLAPQSLETQAPSTTQPQTPTTLTTRPTVTAEQPESVADAAPPAGGSGDDVATLRTKSWFGWWGGQYQELPVDPQGGDRSENREASNSECVADCALSQPRAVPGAEPSIGLEVPLKPANHSQTSSNSVTPLSQTPEASQITPKTSTWAFWSRERSKPSDNSLNSNSTTTVGELAVADTPSQLSPQKAKAREISPISIPDPKMTRTLNNLREESRPGTPKKAEANTSIAATVSAIPKPTPSPSPSSASTAKQKLQKILPANHVLPNFDSCYRRLNQPSIFCQITKIFKSQPPPNKKHLYLTPTPPRIKKAVAIGVHGFFPMRLVRTVLGEPTGTSIRFANSAAAAIKRWAEKNGVEVEIEKIALEGEGKVGDRVEMLWKLLANWMDHVKNADFVLMAAHSQGVVVGVQLLARLIEEGCVDKARVGFTAMAGINLGPFYHLPTTILTGSAKELFEFQRLESIPSKKYIQALRICLAQNVRILYVGSIDDQLVPLESSTFSNISHPYIFRAVFVDGRVHAPDFLSHLVGFAMKLRNLGISDHGLVRELSSPLAGSLYGGEGHSRIYDDNAVYDLGVRHALETTDCPDVPLGLDTFELPTNNNNPFLLPWSMRGMLEEPHVKSKLEGECAELLDQFQKWKPTSKVLKDVKFRLEGIRSKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5GH13</accession>
    <name>D5GH13_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00007651001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430297">
        <property type="protein sequence ID" value="CAZ83806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839615.1">
        <property type="nucleotide sequence ID" value="XM_002839569.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GH13"/>
    <dbReference type="STRING" id="656061.D5GH13"/>
    <dbReference type="GeneID" id="9185060"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007651001"/>
    <dbReference type="eggNOG" id="KOG0143">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010119_6_1_1"/>
    <dbReference type="InParanoid" id="D5GH13"/>
    <dbReference type="OMA" id="FWHVGRE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044283">
        <property type="term" value="P:small molecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000030">
        <property type="entry name" value="Thymine dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050231">
        <property type="entry name" value="Iron_ascorbate_oxido_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47990">
        <property type="entry name" value="2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00682">
        <property type="entry name" value="IPNSYNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="3">
        <location>
            <begin position="186"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ83806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="337" mass="37741" checksum="33A5CF9E1B9BEAC5" modified="2010-06-15" version="1">MVSSNAIGAAVDQDSLFIALIDFSLFLHGDEQERVAVAKGMVDGFKDAGFIYIKNHGIPQEAIDRVFAESATFFKRPKVEKEALAWTSPRSNRGYVSHGREKPSAVQDKAFIDSLRLVNPDLKESFEIGRDDEPEYPNNWPEDPDFVSVMKTFFLTCKDLHIDVMRAIGLGLGLKKGFFDEYTDKGDNTLRLLHYPSTPKEIFVKNKGQVRAGKHTDYGSITLLFQDDRGGLQVRSPVGTFVNAMPIPGTIVINAGDLLARWSNDIIRSTEHQVVEPPIPPESDIYPARYSCAYFCNPNFDKFIEAIPGTYVSEADKKYPGINAGDYLAQRLEQTYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHG8</accession>
    <name>D5GHG8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00007866001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430318">
        <property type="protein sequence ID" value="CAZ83961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839770.1">
        <property type="nucleotide sequence ID" value="XM_002839724.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHG8"/>
    <dbReference type="FunCoup" id="D5GHG8">
        <property type="interactions" value="577"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GHG8"/>
    <dbReference type="GeneID" id="9183606"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007866001"/>
    <dbReference type="eggNOG" id="KOG0294">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030368_0_0_1"/>
    <dbReference type="InParanoid" id="D5GHG8"/>
    <dbReference type="OMA" id="KLHQMKY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051959">
        <property type="entry name" value="PAK1-Kinase_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44675:SF1">
        <property type="entry name" value="P21-ACTIVATED PROTEIN KINASE-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44675">
        <property type="entry name" value="PAK1 INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="428"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="491"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="492"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ83961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="518" mass="55942" checksum="68D47FAB42E37C14" modified="2010-06-15" version="1">MAKRKRPTPSTAAVASTAALSNPTTVVAGNSIVPPTPPALQHASEPPHPPLNFQVITGSYDKVLHGFIVTIPSSSPTSSTPSTSLVPKENDAISLPATFTDSFLFTAHTAPIRTLTISPPSANILQKRILATSSADEHINLYTLSSTLPPINFQKLKKAASTLTASNPKNKNLGVLSHHLNTPTAIIFTPNRSKIITAGLDAQIHILRTRDWAPLSMLKCPKPKPKPINYATDYGDGYGPRIDTFNSEATYGGGAGGVNDIALHPSQKILLSVGKSTRQVRMWNLMTGRKAGVLALSREVVPPIFGSEGTKVVWSKTGEEYAIAFERGVVAFGMDSRPKSRIRTAPISKVHQIRYINLPTTITQNKKDTEEEEVLTISTEDGRVLFYSTTDPESLDSPNLIGFLGGRSIGMPGRVKDFITIAVRNNFYLITGGTDGVVRIWDLGKDSEKDIQEMKKVRIDGGEGRQIGCLVGIYETERRITCLGVMEMGGEGVEEEEDIEMSDSSSSSSGGEDDDDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHU0</accession>
    <name>D5GHU0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00008099001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430321">
        <property type="protein sequence ID" value="CAZ84083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839892.1">
        <property type="nucleotide sequence ID" value="XM_002839846.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHU0"/>
    <dbReference type="GeneID" id="9184176"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008099001"/>
    <dbReference type="HOGENOM" id="CLU_2198911_0_0_1"/>
    <dbReference type="InParanoid" id="D5GHU0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072166" evidence="2">
        <location>
            <begin position="23"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ84083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="108" mass="11483" checksum="11D9AD7D0E0A68FB" modified="2010-06-15" version="1">MPSPLQALLFLLLQSLTTPASAFPRRARLDVSGGSGGSATGRTTVEIKSPGIIAGIALGSIALVIILLAAFIWRRKVWQDRQDQEDWERAGRSMLDGAEPLAAVGRDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHW8</accession>
    <name>D5GHW8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00008154001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peroxiredoxin family. Prx5 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430321">
        <property type="protein sequence ID" value="CAZ84111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839920.1">
        <property type="nucleotide sequence ID" value="XM_002839874.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHW8"/>
    <dbReference type="FunCoup" id="D5GHW8">
        <property type="interactions" value="527"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GHW8"/>
    <dbReference type="PeroxiBase" id="11116">
        <property type="entry name" value="TmelPrxV02"/>
    </dbReference>
    <dbReference type="GeneID" id="9184204"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008154001"/>
    <dbReference type="eggNOG" id="KOG0541">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072440_3_1_1"/>
    <dbReference type="InParanoid" id="D5GHW8"/>
    <dbReference type="OMA" id="ACLLCIK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03013">
        <property type="entry name" value="PRX5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037944">
        <property type="entry name" value="PRX5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013740">
        <property type="entry name" value="Redoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10430">
        <property type="entry name" value="PEROXIREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10430:SF39">
        <property type="entry name" value="PEROXISOMAL MEMBRANE ASSOCIATED PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08534">
        <property type="entry name" value="Redoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 7" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="5 7" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="8">
        <location>
            <begin position="2"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate" evidence="6">
        <location>
            <position position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR637944-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ84111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="156" mass="16578" checksum="17E4263995E59A98" modified="2010-06-15" version="1">MISVGSSIPAAPASLWVEKDNNTVSLPSSGKYIIVGVPGAFTPPCSSHVPGYVENYDKLQAKGISAVYVVAVNDIFVVNAWKEQLAKGSSVHFLSDSKGEFTRLVGLDFDASGLLGNARSQRYVAVVEDGKVTNLQVENEAPNITVTHVDKILSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5GI24</accession>
    <name>D5GI24_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00008238001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430322">
        <property type="protein sequence ID" value="CAZ84167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839976.1">
        <property type="nucleotide sequence ID" value="XM_002839930.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GI24"/>
    <dbReference type="STRING" id="656061.D5GI24"/>
    <dbReference type="GeneID" id="9183500"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008238001"/>
    <dbReference type="eggNOG" id="KOG0266">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_57_1_1"/>
    <dbReference type="InParanoid" id="D5GI24"/>
    <dbReference type="OMA" id="CKGHDTA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000228">
        <property type="entry name" value="COMPASS-like H3K4 histone methylase component WDR5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059122">
        <property type="entry name" value="Beta-prop_WDR5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001632">
        <property type="entry name" value="WD40_G-protein_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848:SF8">
        <property type="entry name" value="F-BOX AND WD REPEAT DOMAIN CONTAINING 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25175">
        <property type="entry name" value="Beta-prop_WDR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002394">
        <property type="entry name" value="GN-bd_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00319">
        <property type="entry name" value="GPROTEINB"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="WDR5-like beta-propeller" evidence="5">
        <location>
            <begin position="36"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="37"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="79"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="121"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="163"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="206"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="294"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ84167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="334" mass="36617" checksum="961D376E7D1CB11B" modified="2010-06-15" version="1">MSTQEPSSSVQPEQADIKMTDPSNARTVPNYALTYTLKGHKMGVSSVKFSPDGAWLASCSADQTIKVWHAKTGKYEQTLEGHMAGISDIDWAPDSLTLVSGSDDKTLRLWDVVSGKMLRLLRGHHNAVYTVAFSPRGNIVASGSYDEAVRLWDIRSGKCMKTLPAHGDPVSGVHFNRDGTMIVSCSHDGLIRIWDVTTGQCLRTLVEEDNAPVMAVKFSPNGKYLLAGTQDSCVRLWDYHRGKCLKTYMGHKNDKYSIFSTFIIANGSCFVMAGSENSDVFIWDIQTKEIVHLLVGHPDVVLGVDSHPTENIVASCGLDGTVMVWMDSAEGLDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5GI35</accession>
    <name>D5GI35_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00008250001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430322">
        <property type="protein sequence ID" value="CAZ84178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839987.1">
        <property type="nucleotide sequence ID" value="XM_002839941.1"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GI35"/>
    <dbReference type="GeneID" id="9181896"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008250001"/>
    <dbReference type="eggNOG" id="KOG0988">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001366_0_1_1"/>
    <dbReference type="InParanoid" id="D5GI35"/>
    <dbReference type="OMA" id="DDYLVIW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031380">
        <property type="term" value="C:nuclear RNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003968">
        <property type="term" value="F:RNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030422">
        <property type="term" value="P:siRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007855">
        <property type="entry name" value="RDRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057596">
        <property type="entry name" value="RDRP_core"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23079">
        <property type="entry name" value="RNA-DEPENDENT RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23079:SF55">
        <property type="entry name" value="RNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05183">
        <property type="entry name" value="RdRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RDRP core" evidence="2">
        <location>
            <begin position="660"/>
            <end position="1237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="376"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1486"/>
            <end position="1542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="144"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="382"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1493"/>
            <end position="1515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ84178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1542" mass="174177" checksum="78F6E0F60F7B9C49" modified="2010-06-15" version="1">MAPPAAAMSHHRAENAQPVSSSGSTLNPRANPYLAVSSISQLALLTQQEVQQAATASGNGDRPVQSTLTNGASRPASIRTAPLRGNPISSRRGYPGPLGRGSPTRSRNNDEFHRRSYNDRALPMLTSLPSRSEILAPRPTTRNTGDRGDVGRGYHNGRENNRPSHSNSHPTPYTDPTNHSLSENTVSMASATNIQNGNNTHSSRGLPILMQNDWKKTDEISIEISGIPLPLDLYNLYDIFSEYGEIEYLKLYHNDSGVPDGNGRISLSNVERPFWEEPFRYSLGVQGPNGSLKLRLDSRRNKQRTVQSPVDRQKSYPQKYSFKLALLQFGILHQEDKMVPMFTTTGQDEASLDVDLHRRELDIVFKIWLKNDASAQTNQSQEEVEGDVNNYDDDDSNAPENKPTERFERYRFGVPFSHLEKLVEVETLSAPGRAFLIDLPSAPPFWRKLHNPRLSIDDRIKYWTSRNAWFRQTSITFDNSARKGLPISLEQRGQTINIGRWTTYRFEVGTKEAGSDGFNEMISALKDFNIKLDVQKDFKTLSEKFDPIWDYIRIKRIQSSVSFLDQLHQASSPLLSYPVRYQLEVCISHNCLSEYNITCEFIDRLAALPKEAAIPLLEHIAERRIRHYDPMDIFTIQGVTKGTINLKLPDHCQLLRKANVTPSTVYFTTPTVEITNRVVRQYSEHIDRFLRVQFVDEKSQGRINSTDKKTMHEVFTRVKAALDHGITIGDRKFEFLAFGNSQIREHGAYFFASDDHLSAADIRAWMGSFSEIRIVAKHAARLGQCFSTTRAINGVRVDVRTTEDIVNAEGYNFSDGVGKISEFLARLITTELRLRLVDKEPVPSCFQFRLGGRKGVLAVSPDANHREVYIRPSQLKFEARHNILEVIRWSKFATAALNRQLIIVLTSLGAPDHYFEDKLQFMLKSYEVAMVDPGVAVRLLCRNVDPNESTLAIAAMIRAGFMDRREPFVMSLLKLWRAWTIKYLKEKAKIMIEEGAFVLGVVDETGTLDGYYYSQDEGSTEDADNPETSLPQIFLQVTDPDRPGESRIIEGPCVIARNPSLHPGDVRKVMAVNAKELHHLRNVVVFSQKGDRDLPNMLSGGDLDGDDYIIIWDKKLTSCVNNYEPMNYSAQTPRELARDVEIGDITKFFVNYMKNDKLGTIANAHLAWADKRDEGVKSDECFRLAILHSHAVDYVKSGEPAIMPPELRRKTFPHFMEKKPEETYRSDKILGVLYDSVSKIDFVPALELEFDDRILNAYTLNEEILEVARAIKIRYDAAMRRIMAQHDIHTEFEVISIFIMHHSMATKDYKFHEDIASVCNGLKDTFKKIIYEVVASKDDIIMGPFIAAMYFVTHQEMAKAKKKFFAAKAKKAAMRAAKRASGNEKAQREGNIPSCTKDLEELAEFTIEEARNMPLISFPWLFGRTLGGIANREYPFAKLDGSPVNFKQAAAVTDELEGRMGKKEKYATYHHGIYDDAVAATTPIIRGNETDTPDNLWNGESSLGISQRGTNPEDVTTTTTDKIIPTSPDSNTGYDGGEANLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5GI84</accession>
    <name>D5GI84_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008329001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430324">
        <property type="protein sequence ID" value="CAZ84227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840036.1">
        <property type="nucleotide sequence ID" value="XM_002839990.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GI84"/>
    <dbReference type="STRING" id="656061.D5GI84"/>
    <dbReference type="ESTHER" id="9pezi-d5gi84">
        <property type="family name" value="Fungal_carboxylesterase_lipase"/>
    </dbReference>
    <dbReference type="GeneID" id="9181945"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008329001"/>
    <dbReference type="eggNOG" id="KOG1516">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006586_14_0_1"/>
    <dbReference type="InParanoid" id="D5GI84"/>
    <dbReference type="OMA" id="NTRCING"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050309">
        <property type="entry name" value="Type-B_Carboxylest/Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11559">
        <property type="entry name" value="CARBOXYLESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carboxylesterase type B" evidence="1">
        <location>
            <begin position="13"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="536" mass="58469" checksum="C003C81484BC3396" modified="2010-06-15" version="1">MATVSHTSLHSRLTGISLFGDRVVQFRGLKFASVPRRFARAELFEGYPSELDCTRHGPICPQEPTSLESDLFGIPAPLQKGKLGLTFDELECLNLAVTLPKSHVPNILSGKKLPVLVSIHGGSNRVGAGCSSLQDPAGLSAASVTEGKEVICVAINYRLNVFGYGVLPDGEGSNNGLFDQQIALVWVKRHISGFGGDPGNTTLAGESAGSLGVDAQLHASSPIGRGLFKRAIMQSGCLDTGAPKPREKMIQTTRRVADFLERGSGEKDEDWVEKLRNASVRELVSASIKAKVGMWCVSDDGTFFTSPWDQAAGIPDWCESLIIGDCDFETPTQGFVWYLQIAPASAAIIHQAFTGVQPHNTRVLEAYKVPAPAPDSASALRAAAFAFIGDSVFSYPAHKLASRWRSAGKRVYEYCFDQPNPYTPEFRRAHHGVDLLYLYPGYELPEKDRVVAEAFQKHVLEFCHSGNAWKASDGQVMGYGPAGEVRIVEKKERRRVAEWEASLEFLRRAELPKAIEYVKEYLGAAKRAIEGETVIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIN9</accession>
    <name>D5GIN9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein transport protein SEC31</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Protein transport protein sec31</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">GSTUM_00008590001</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">COPII-coated vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat SEC31 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430328">
        <property type="protein sequence ID" value="CAZ84382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840191.1">
        <property type="nucleotide sequence ID" value="XM_002840145.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIN9"/>
    <dbReference type="FunCoup" id="D5GIN9">
        <property type="interactions" value="700"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GIN9"/>
    <dbReference type="GeneID" id="9183316"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008590001"/>
    <dbReference type="eggNOG" id="KOG0307">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003033_2_0_1"/>
    <dbReference type="InParanoid" id="D5GIN9"/>
    <dbReference type="OMA" id="WLERPCG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007029">
        <property type="term" value="P:endoplasmic reticulum organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000193">
        <property type="entry name" value="Protein transport protein SEC31, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.940.10">
        <property type="entry name" value="Functional domain of the splicing factor Prp18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040251">
        <property type="entry name" value="SEC31-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13923">
        <property type="entry name" value="SEC31-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13923:SF11">
        <property type="entry name" value="SECRETORY 31, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <keyword evidence="7 15" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="100"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="237"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="464"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="782"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="847"/>
            <end position="1168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="810"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="855"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="887"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="931"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="964"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1009"/>
            <end position="1078"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="1086"/>
            <end position="1105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="1118"/>
            <end position="1135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="1142"/>
            <end position="1156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="CAZ84382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1235" mass="132592" checksum="F0160F1459DEE8CB" modified="2010-06-15" version="1">MARIRDIQRTAAFTWSPGTAPFIATGTQAGAVAADFSDNTQLELWDLGLGNAHAGGELAPVASITTDSRFYDIAWGKVSNDKPRGIIAAIVGSDPLISRTTKHMGSIKSLQFNPFKSELLATAGAKGELYVWDLNNTENPFLLGNRAARADDFDCVDWNKKVPHILVTGGNGGFVTVWDVKSKKESLTLNNLGRKAVSAVAWHPEQQTKLITAIPDDTNPVILLWDLRNSNAPERTLSGHELGVLSLSWCKQDPDLLLSCGKDNRTICWNPQTGEKLGEFPVVTNWTFKTQWNPRNPSLLATASFDGKIVVQTLQNTNPTSSQTDNLGATQPDTDDFFSRASNAQTSSFTLKQPPKWHCPPVGASFGFGGKLVSLTFPEPQAGQPRRSVVKISTFAVDSGVSSATKSFEKALSEGNLVNVCDLRSQDAKTDEEKADWKILRTLFEGNPREKLIEYLGFKEEDLEDTDAGVDTKSTDGEGENQSLDQSDGPAAHDKRLSSFFADAGADSENFLADLSSIQSTRGAKANNPFQIFTGNESEADRKITRALVLGQFEKAVDVCLKEDRISDAFMLAICGGEKCIEKVKTAYFTKNVKGPNYLRVLASIVGKNLWDVVHNADLANWREVMVALCSFADEKDFPDLCEALGDRLEQELGLAEGKKEARKHASFCYLAGSKLEKVVDIWIESLYEGEKAGLEETSDDSAFAIHARSLQNFIEKVTVFREAVKFSDEEKNLSGGWRLRVLYEKYCEYADVVASHGQLEVAERYLDLLPTNYPAATVTRNRVKEASGKGVTAAQARKPQQPVATGYGSQQHQASSSPYQPQQPAAVATGLANPYAPPAPVAAPVNPYGAPTTTPQPGPYAPPAPPAGANPYAPPTVTQGYKPGPYQSTQPAQISGQYGTGYPGYNATGFGQPPPQRNFGPATSVPPPSQASNMANWNDTPNVTKVPRRGTPSATAPVTSPFPGAPGAPSPPTTSMPFGAPPPRGSSAPPPPKNAAPPQRVQSPAQHQFRSQSPAAQAQAPYSPAAQAFPPSQPSYASAPPPTAAASRYAPAPAAADAAPQPRQGQAPPPQGAFIGARQVGPGPQYAPPPTAFGQPPAPSPYAPAPSQNQGGQYGAPPGPGQYGPPPTQRPPSQPAQLEAAPPPKAQTPVPPPSKHPAGDRTHIPSQYLPIFELLTSDFERVKQRAPPQFQRPVKDTERRLNILYDHINNEDVLSQEALESMLELSRAIPNILA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIV3</accession>
    <name>D5GIV3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00008700001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840255.1">
        <property type="nucleotide sequence ID" value="XM_002840209.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIV3"/>
    <dbReference type="GeneID" id="9185664"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008700001"/>
    <dbReference type="eggNOG" id="ENOG502S4TY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052067_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIV3"/>
    <dbReference type="OMA" id="YLYFLDI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044695">
        <property type="term" value="C:Dsc E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032933">
        <property type="term" value="P:SREBP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038967">
        <property type="entry name" value="Dsc4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013715">
        <property type="entry name" value="DUF1746"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39405">
        <property type="entry name" value="DSC E3 UBIQUITIN LIGASE COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39405:SF1">
        <property type="entry name" value="DSC E3 UBIQUITIN LIGASE COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08508">
        <property type="entry name" value="DUF1746"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="93"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1746" evidence="3">
        <location>
            <begin position="44"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="173"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ84446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="280" mass="31927" checksum="EC233A8BBFC04CD5" modified="2010-06-15" version="1">MSDARDRQPPAATQPEEDQERNQDRYFKILRYKRSEFLDHLWRNLDRITYCYFGYLYYLDCSFFRFFLRCAIQMNYLTPKPMGLPMVPQKRSDIIALFGSGGFCFLWHVITSPPSAGELTNGYLHGGLIIDFIGQEGPISKWRLVYMDFAILFLQLVMLTVIAVKHDHDAESVRIEEGRQPPQDLDSEERGEARRYSTSSHGSFGGGSARVQGDEEAVLMNGDEPVAGSAETNMQKRTFEISSGEYLIAKIDIVEHVKKQWNKGATVTTDAPAMPHLRPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJX4</accession>
    <name>D5GJX4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00009255001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutaredoxin family. Monothiol subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430334">
        <property type="protein sequence ID" value="CAZ84817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840626.1">
        <property type="nucleotide sequence ID" value="XM_002840580.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJX4"/>
    <dbReference type="FunCoup" id="D5GJX4">
        <property type="interactions" value="18"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJX4"/>
    <dbReference type="GeneID" id="9186853"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009255001"/>
    <dbReference type="eggNOG" id="KOG1752">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026126_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJX4"/>
    <dbReference type="OMA" id="PNIMINK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005796">
        <property type="term" value="C:Golgi lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004362">
        <property type="term" value="F:glutathione-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03419">
        <property type="entry name" value="GRX_GRXh_1_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000093">
        <property type="entry name" value="Glutaredoxin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011899">
        <property type="entry name" value="Glutaredoxin_euk/vir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014025">
        <property type="entry name" value="Glutaredoxin_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02180">
        <property type="entry name" value="GRX_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45694">
        <property type="entry name" value="GLUTAREDOXIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45694:SF5">
        <property type="entry name" value="GLUTAREDOXIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00160">
        <property type="entry name" value="GLUTAREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutaredoxin" evidence="2">
        <location>
            <begin position="103"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ84817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="203" mass="22076" checksum="D7D45BC363F32C82" modified="2010-06-15" version="1">MFLIVIYYNSGRDAVKRETYMQTMQKAALDRENAGVGNRLREAADAAKKAADMKAGPKPDSPRAVEAVMKGRLEQGPIGKEGGDGGGHSAIDELAGILKRSPIIIFSKTYCPHSRAAKDLFLHAYKIVPAPHVVELDSHPHGAELQDLLAKQTGRRTVPNIMISGKSIGGNDDVQQLEREGRLGDTIHSMGGKRIMKVLKLKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5GK62</accession>
    <name>D5GK62_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00009383001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="FN430336">
        <property type="protein sequence ID" value="CAZ84905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840714.1">
        <property type="nucleotide sequence ID" value="XM_002840668.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GK62"/>
    <dbReference type="STRING" id="656061.D5GK62"/>
    <dbReference type="GeneID" id="9186943"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009383001"/>
    <dbReference type="eggNOG" id="KOG0332">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_1_0_1"/>
    <dbReference type="InParanoid" id="D5GK62"/>
    <dbReference type="OMA" id="REYAIME"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="47"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="225"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ84905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="386" mass="43520" checksum="7FFC3CAEBD085AF8" modified="2010-06-15" version="1">MGVSDVDEPKYEVAVRFDDLQGDLYSPFYSVKRFEDLGLQDLLEGIYFMNFKKPNMIGQSQSGTGETAALVLTMLTRIDMSVSNVEALCLAPTRELAKRIQRVVQTMGQFTNVKAQFAIPNLVKRGEKIDAHIVVGTPSTVLDLIQRKQLAVELLKVFMLDEVDNMLDLQGLGEQCLRVKRNIPSTTQIALFSATCSDRVFKYMYRFAPNANRITSKNTQPSLAGIKQLCMYCRSEEDKYRVLLDLYHVLTVGSSIIFSKKRETASEIQRRMELDGHKVATLHSAQEGPDHDKAVHAFRSGKAKVLITTDAFARGIDLETVSMVVNYDLPLDQNRRPDPVAYLHRVARTGIFGRPGLSVNFVHDHESLYQVNEISTHFGTCMTRIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKH5</accession>
    <name>D5GKH5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Splicing factor YJU2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00009561001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Part of the spliceosome which catalyzes two sequential transesterification reactions, first the excision of the non-coding intron from pre-mRNA and then the ligation of the coding exons to form the mature mRNA. Plays a role in stabilizing the structure of the spliceosome catalytic core and docking of the branch helix into the active site, producing 5'-exon and lariat intron-3'-intermediates.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the spliceosome. Present in the activated B complex, the catalytically activated B* complex which catalyzes the branching, the catalytic step 1 C complex catalyzing the exon ligation, and the postcatalytic P complex containing the ligated exons (mRNA) and the excised lariat intron.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the CWC16 family. YJU2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430339">
        <property type="protein sequence ID" value="CAZ85018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840827.1">
        <property type="nucleotide sequence ID" value="XM_002840781.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKH5"/>
    <dbReference type="FunCoup" id="D5GKH5">
        <property type="interactions" value="676"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GKH5"/>
    <dbReference type="GeneID" id="9182037"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009561001"/>
    <dbReference type="eggNOG" id="KOG2989">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053603_1_1_1"/>
    <dbReference type="InParanoid" id="D5GKH5"/>
    <dbReference type="OMA" id="ENCDYQN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071006">
        <property type="term" value="C:U2-type catalytic step 1 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000349">
        <property type="term" value="P:generation of catalytic spliceosome for first transesterification step"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03226">
        <property type="entry name" value="YJU2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007590">
        <property type="entry name" value="Saf4/Yju2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043701">
        <property type="entry name" value="Yju2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12111">
        <property type="entry name" value="SPLICING FACTOR YJU2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12111:SF1">
        <property type="entry name" value="SPLICING FACTOR YJU2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04502">
        <property type="entry name" value="Saf4_Yju2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="115"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="115"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ85018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="264" mass="30873" checksum="97A420A40EE44581" modified="2010-06-15" version="1">MSERKVLQKYYPPDFDPSKIVRHKGPKPTGPKQQTVRLMAPFSMKCLNCGEFIYKGRKFNARKEHSGEKYYNISIFRFYIRCTRCSSEITFKTDPKNMDYTCEKGAIRNFEPWRDTREKDETEEERLNRLEEEENESAMAKLETKTLDSKREMQIADALDEIRTRNARNERFDSADVLDTLLEVPDPAELELMRQEEEDAELARKAFRTEEDKFVRRVVDDGADGTDPPKEGVKADIPSFKKTVKRRRDMGTALGIKKKLKPLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKT7</accession>
    <name>D5GKT7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00009764001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430341">
        <property type="protein sequence ID" value="CAZ85130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840939.1">
        <property type="nucleotide sequence ID" value="XM_002840893.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKT7"/>
    <dbReference type="FunCoup" id="D5GKT7">
        <property type="interactions" value="882"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GKT7"/>
    <dbReference type="GeneID" id="9183366"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009764001"/>
    <dbReference type="eggNOG" id="KOG1273">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032142_0_0_1"/>
    <dbReference type="InParanoid" id="D5GKT7"/>
    <dbReference type="OMA" id="DYEDDIM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037850">
        <property type="entry name" value="RBBP5/Swd1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44040">
        <property type="entry name" value="RETINOBLASTOMA-BINDING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44040:SF1">
        <property type="entry name" value="RETINOBLASTOMA-BINDING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="63"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="422"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ85130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="472" mass="52683" checksum="D8F3FDD497635120" modified="2010-06-15" version="1">MNLSLLDPFTLAQDYPESLSSELRSGHSTCVRFNRKGDLIASGRLDGTVVLFDVETNGLARYLRGHVRGIQSLSWSQDGIFLASASQDFKVIIWDLSKQGEGASRTIRFPAPVYCAELHPKNPNIFVCALFEDSPYLVDISDHVPRKYQLTSAPKRPLQPGAEEGEIDEKRAAADAKQMTTASVFTATGDHIITGSNKGWVNVISTTTKEMIHSTRVTSGCITYIRLTMSGRNMVVNANDRVLRSINIPDNLKEGVDGNDVRLIVEHKFQDVVNRLLWNHCTFSGNGDYLTATTFKNHDIYIWERSIGVLVKILEGPKEELGTVEWHPTRPLIAATGLESGKIFIWATSNPQRWSALAPDFRELEENIEYQEREDEFDIHLKWDLTKRKLHQENEIVDAVTIERARGEVEDGWNMPVVLDLEDTESESDSPPPSGPGERKRKSPVPSKANGNNAGANGNGTGTTRSKRRRAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKX4</accession>
    <name>D5GKX4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00009811001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430341">
        <property type="protein sequence ID" value="CAZ85167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840976.1">
        <property type="nucleotide sequence ID" value="XM_002840930.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKX4"/>
    <dbReference type="STRING" id="656061.D5GKX4"/>
    <dbReference type="GeneID" id="9183403"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009811001"/>
    <dbReference type="eggNOG" id="KOG0714">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043818_0_0_1"/>
    <dbReference type="InParanoid" id="D5GKX4"/>
    <dbReference type="OMA" id="FSAWGRY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051787">
        <property type="term" value="F:misfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051948">
        <property type="entry name" value="Hsp70_co-chaperone_J-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44360">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44360:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="154"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="190"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="3">
        <location>
            <begin position="76"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ85167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="343" mass="39266" checksum="E416B3AEABB9386B" modified="2010-06-15" version="1">MSALLSFIGWSFLPSLATSSFLSFWYRLITRAGDPRPQPGTPTYVKSYKLVNFLVIAFYLLYTIYESYNAILLEPNFYKLLSAGMRASDRELKGKFRRLTVMYHPDKVGTQGEAYFVMLKAAYDTLIDPVKRSAYDRFGPDMLEWRNCSSAYDYLIRGVTMMGPYYLGSVLVLVILNVLGKMEFGRYWRFYAFFCMMVLEAFVVTRPFHHFTQIPILNTLLPFEQLALARKILFTSFIAISQLAPLFQQDRAIAGPLTAEALDPQLDRMAALLHRTDTETSTILTQELLPFDANPVMQKMLKKKLEEWMIESAIRSAPEVRDAVGRVIQRRRTDAPAGAQGTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5GL24</accession>
    <name>D5GL24_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00009926001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430344">
        <property type="protein sequence ID" value="CAZ85217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841026.1">
        <property type="nucleotide sequence ID" value="XM_002840980.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GL24"/>
    <dbReference type="GeneID" id="9185811"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009926001"/>
    <dbReference type="eggNOG" id="KOG0703">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017628_0_0_1"/>
    <dbReference type="InParanoid" id="D5GL24"/>
    <dbReference type="OMA" id="ASWNMGI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08204">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.150:FF:000026">
        <property type="entry name" value="GTPase activating protein for Arf, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051718">
        <property type="entry name" value="ARF_GTPase-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45705:SF7">
        <property type="entry name" value="ACTIVATING PROTEIN FOR ARF, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G09120)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45705">
        <property type="entry name" value="FI20236P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Arf-GAP" evidence="4">
        <location>
            <begin position="14"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="3">
        <location>
            <begin position="199"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="134"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="332"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="402"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="534"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="138"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="166"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="333"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="409"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="424"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="545"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="561"/>
            <end position="570"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ85217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="632" mass="68463" checksum="9D253D9348AC7698" modified="2010-06-15" version="1">MSSIKNKRQQARNERILQDLIKSVPGNDRCADCGTRNPAWASWSLGIFLCIRCASLHRKLGTHISKIKSVSMDMWTNDQIDNMRSRGNVISNTIHNPDASKHPVPLNGEDSDSLMERYIRNKYEYKLFMRETAQPKNSSTSSISSGASSSTGAPPTRSVSAGYVASRNPPVRTSSPSSSSSKAPPAGPVVFPHGMVVAPPYQEQLQSLKDMGFKDERKIMEVLKSVNGKIIEASEILLRLGNNSSPSDNPPAGISIQKSGGSVNPFDALDRELPPLPPGATEVGGIKPQNVPPQIVPQQQQQQVSMGYQNSYQTRAPAPATSYGSGHQWFDTQQQSQQQVNGNTAYGQGQGYQGFAQQPQQMQTPQTNPYASFTTSAVTTSTPATAGSVNINGGIGGYNPFLSGSAPPIQKPYNPPTQPPVPQQNPYLQQQQAQQQQQAQLRQQQLQQQQQAQQQIQQQTQQQAQAQTRTQIQVQLQQQQALYGSQQQWSPAPATLSYPQQLQMNPYQSNLQAQQPPIPQRVGIDKSSILALYNYPHLAPPPNNNPAQQQQQESQVPVSNAPQSLATTFANGGAAPAPVTHGAGSNNPFLPSAPPVGAGGGRGHMSQESVDFGAWQSGRHSPDAFASLSFRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5GL80</accession>
    <name>D5GL80_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00010052001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430346">
        <property type="protein sequence ID" value="CAZ85273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841082.1">
        <property type="nucleotide sequence ID" value="XM_002841036.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GL80"/>
    <dbReference type="STRING" id="656061.D5GL80"/>
    <dbReference type="GeneID" id="9182067"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010052001"/>
    <dbReference type="eggNOG" id="KOG1477">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077693_1_0_1"/>
    <dbReference type="InParanoid" id="D5GL80"/>
    <dbReference type="OMA" id="GREACYE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd12885">
        <property type="entry name" value="SPRY_RanBP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044736">
        <property type="entry name" value="Gid1/RanBPM/SPLA_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050618">
        <property type="entry name" value="Ubq-SigPath_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12864">
        <property type="entry name" value="RAN BINDING PROTEIN 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="1">
        <location>
            <begin position="1"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="207" mass="22684" checksum="64F0AE52B2DA27E8" modified="2010-06-15" version="1">MHKIPSPSLFTDEDKAPGLIIEDNGLTISLPVRWGSAAQCARANTFIPVDHDRMYYYEMTVVDEGDGKRIGLGFCARDTSLNGMPGWEVDAWGYHADDGAKFPGRSQGLKYGPVYGTGDVVGCGLDFKRGFAFYTLNAEYLGPAFSLKEGLRIWPMIGMGSAGAKVSINFGQRKFTWDGIDDLEGRLGDNATIEYGREACYENCGLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLH9</accession>
    <name>D5GLH9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dihydrofolate reductase</fullName>
            <ecNumber evidence="2">1.5.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00010215001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the dihydrofolate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.3" evidence="2"/>
    <dbReference type="EMBL" id="FN430349">
        <property type="protein sequence ID" value="CAZ85372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841181.1">
        <property type="nucleotide sequence ID" value="XM_002841135.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLH9"/>
    <dbReference type="FunCoup" id="D5GLH9">
        <property type="interactions" value="403"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GLH9"/>
    <dbReference type="GeneID" id="9186116"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010215001"/>
    <dbReference type="eggNOG" id="KOG1324">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043966_2_1_1"/>
    <dbReference type="InParanoid" id="D5GLH9"/>
    <dbReference type="OMA" id="QYEFQMW"/>
    <dbReference type="UniPathway" id="UPA00077">
        <property type="reaction ID" value="UER00158"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004146">
        <property type="term" value="F:dihydrofolate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046452">
        <property type="term" value="P:dihydrofolate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046655">
        <property type="term" value="P:folic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00209">
        <property type="entry name" value="DHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.430.10">
        <property type="entry name" value="Dihydrofolate Reductase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012259">
        <property type="entry name" value="DHFR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024072">
        <property type="entry name" value="DHFR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017925">
        <property type="entry name" value="DHFR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001796">
        <property type="entry name" value="DHFR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48069">
        <property type="entry name" value="DIHYDROFOLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48069:SF3">
        <property type="entry name" value="DIHYDROFOLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00186">
        <property type="entry name" value="DHFR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00070">
        <property type="entry name" value="DHFR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53597">
        <property type="entry name" value="Dihydrofolate reductase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00075">
        <property type="entry name" value="DHFR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51330">
        <property type="entry name" value="DHFR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHFR" evidence="8">
        <location>
            <begin position="7"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ85372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="202" mass="22354" checksum="A6FAF9282A3273E3" modified="2010-06-15" version="1">MALQNLNLTVIVATTHSLGIGKSGTLPWHLPTDLSYFSRVTKRPPPSQSPSTSPPVLNTLIMGRKTYFSIPPKFRPLPSRRNIVISRSSRPDSFNDASTWVTSFPDAIAAAGGDGGRIFVIGGAEIYKLAMEDERTRNVLLTRVEGEFGCDRFFPVDVRDEGNTGWAQRGHEELCAFVGEAVPAGAREENGVRFWFELYQRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLN3</accession>
    <name>D5GLN3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00010283001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430350">
        <property type="protein sequence ID" value="CAZ85426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841235.1">
        <property type="nucleotide sequence ID" value="XM_002841189.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLN3"/>
    <dbReference type="FunCoup" id="D5GLN3">
        <property type="interactions" value="810"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GLN3"/>
    <dbReference type="GeneID" id="9182729"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010283001"/>
    <dbReference type="eggNOG" id="KOG2570">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023529_2_0_1"/>
    <dbReference type="InParanoid" id="D5GLN3"/>
    <dbReference type="OMA" id="NFRCNEP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd10568">
        <property type="entry name" value="SWIB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.245.10">
        <property type="entry name" value="SWIB/MDM2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019835">
        <property type="entry name" value="SWIB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036885">
        <property type="entry name" value="SWIB_MDM2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003121">
        <property type="entry name" value="SWIB_MDM2_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13844">
        <property type="entry name" value="SWI/SNF-RELATED MATRIX-ASSOCIATED ACTIN-DEPENDENT REGULATOR OF CHROMATIN SUBFAMILY D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02201">
        <property type="entry name" value="SWIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00151">
        <property type="entry name" value="SWIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47592">
        <property type="entry name" value="SWIB/MDM2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51925">
        <property type="entry name" value="SWIB_MDM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DM2" evidence="2">
        <location>
            <begin position="272"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="171"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="41"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="171"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ85426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="492" mass="56125" checksum="D4C797302CF38477" modified="2010-06-15" version="1">MEHHPRPSPHPQRVSHGQGYGQPTTARRGPGPMLGNTAVPHQSALSQQQLHQQHQTAMAERELAKRRARKPTDKMIPDGVDEIVPNVALYKELRDMERRYDATILRKRLDVQDAVNRNVKNYKTLRIFISNSAKDQPWQNSDRPLDENAFDFDTGQIPTFRVRIEGKLLEDEEDDAAEVDPDSDLNLEPTPGFTDPIQPPKKKFSHFFKGITVELDRNKDIHPEGNVIEWRKTAPNVPPGAAPQGPLEFDGFEFERKGDENVNCTIKLIRDEVPDRFRLNSALADLLDTKEDTRAGIVMGIWEYVRINGLQDPDERRTINCDENLKKIFAQDRLYFPQIPELTLAHILPLEPISINYTIRCDVPQHFSPAVYDVTVSIDDPIRAKMSALLTSPSNLQGLREISTIDEQIALLVQSINHSKAKRDFWHAMSVDPADFVKRWVSSQKRDLDTLNGEAIGVGVEDEEMRRKEFFDKVGENVYLLLSRQVQKQGGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLR9</accession>
    <name>D5GLR9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00010375001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430351">
        <property type="protein sequence ID" value="CAZ85486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841295.1">
        <property type="nucleotide sequence ID" value="XM_002841249.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLR9"/>
    <dbReference type="STRING" id="656061.D5GLR9"/>
    <dbReference type="GeneID" id="9186470"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010375001"/>
    <dbReference type="eggNOG" id="KOG1176">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_2_1"/>
    <dbReference type="InParanoid" id="D5GLR9"/>
    <dbReference type="OMA" id="CSIQQWI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016405">
        <property type="term" value="F:CoA-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05911">
        <property type="entry name" value="Firefly_Luc_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000007">
        <property type="entry name" value="4-coumarate--CoA ligase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096:SF424">
        <property type="entry name" value="ACETYL-COA SYNTHETASE-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="2">
        <location>
            <begin position="30"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="3">
        <location>
            <begin position="470"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ85486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="569" mass="62858" checksum="56F3A74A976DE1F2" modified="2010-06-15" version="1">MPISSRWSCDIPKCSLTTYLFESPTAPLPKEPLYIDPLDPKKFVSYHAYRSLVQRIATGLLAFGLRHGDRVVLFSGNNIFFPSFFNGIVAAGGIFTGANPSFLLREIEQQIRSSGARFLVAHRSNSGIAREAAENVGLDEGSVFMFDEGLDITADGKILGWGDKSSPGRTRHWSDIVEKGYDGFVWQELKTDEELDTTAAINYSSGTTGVPKGAQITHRNIVTNAHQVVSLRMQSNSAPNDSRWLAMLPMYHVYGQTYYAIIAPKLRALVYMLQKFDFGAFLTTIQKHKITTIAAVPPIMVALAKHPDVIKFDLSSLNVLGCGSAPLSRDISREVEERVMRGRAGEERVNLKQGWGMTEATCSVTGFHPDDTDEEGSVGELLPNCEGKVMDAETATRELPPNTQGEIWVRGPIIMKGYYRNKAATEDTITPDGWLKSGDIGYYNEAGRWFIVDRKKELIKVKGNQVAPAELEGMLLEHPSVADAAVVGIPHAEDERPRAYIVKKPGTTVTADEISEWVKKRAIRYKWITGGVIFIPAVPRNPSGKILRKGLRDRAKTETRDSGNVAAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>D5GM71</accession>
    <name>D5GM71_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="11">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00010551001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430352">
        <property type="protein sequence ID" value="CAZ85614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841423.1">
        <property type="nucleotide sequence ID" value="XM_002841377.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GM71"/>
    <dbReference type="GeneID" id="9187398"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010551001"/>
    <dbReference type="eggNOG" id="ENOG502S2AR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_322669_0_0_1"/>
    <dbReference type="InParanoid" id="D5GM71"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051061">
        <property type="entry name" value="Zinc_finger_trans_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46179:SF13">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46179">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="629"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="575"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="719"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="875"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="37"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="748"/>
            <end position="763"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ85614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="897" mass="98929" checksum="2A5FC59E55AD14B8" modified="2010-06-15" version="1">MLGRSNTTTPAGDPEVTKKHIDRVNSRMNPILESIEATGSRSQPLNEDQMEVDPLPGIFCDTTMSSSSTASDSWMVGEEISDNDPEVINSIDDLERAFDDCLPNSSDTMDGIEGSDKDTLLKPEDVASPLHKPLLHTSHSVHESPETLRVGLVAAQDHKHPKSDSGIGSTQGSPGNAFLYKEYKGPDYEGSGRLFFGELPSCDTGNPVKEVYSVRVEGSGPSRNGSPGSSSCTVEASETIANYNGHHESPPQLDEIHTSYGPTVFHYGEPTPPGSAIRSPSAYSFFEVRDFPKTKQLSTQSVEAMVQYILNPILLDEAFEDFWQLCEQAKRGIFQGKICWLRDLEKFLFDGIPAVRSLSSYKAFCETFLSNCQRALPYIRELDQQRSYDPVYSSAYFIDALHRAKESADAWTAEKNKVMRMANYDSQSVSEELDTSGNNKSKLKDFVPLPNIISSEGRKRKMPIDIGHDESQMKRALTPNKAAGSAQPRLAIQTTAPLGYFDGYHDALPSSPLRVENIGYPLNPRFDFPPRVLTAAQSGKFSEYTHPSSESTAANATALLPSDVSGDHRVMTRHSIGYPQAPTPADTNYSGSPAAPASFEGHSGSSHGTSRRKASVPAGKPDEEDAPIYRCDQCPREFPRPCDLTKHKKTHERPFKCDDEGCKYHIEGFPTNKERERHQNDIHVANSKEWKCTYSPCAYKSKRESNCKQHMEKAHGYNYKRMKRNPRKRPEQLTKPVTSDKKKRGAIRKASVATSRSTTVSKAIKASRSSRPEAPEATALPGQDSNVAVTTGFDMYQGYPQSPTIHGTSDEYQLLDTEMPPTSYFDGSPPTNFVPSPADAPIGYNPPMDAYQANHPMMGNSGWFSSQSSSFLDPFLSEFEQSSAPYDDGVGDDDTAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMQ1</accession>
    <name>D5GMQ1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00010877001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430358">
        <property type="protein sequence ID" value="CAZ85794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841603.1">
        <property type="nucleotide sequence ID" value="XM_002841557.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMQ1"/>
    <dbReference type="FunCoup" id="D5GMQ1">
        <property type="interactions" value="44"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GMQ1"/>
    <dbReference type="GeneID" id="9185130"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010877001"/>
    <dbReference type="eggNOG" id="KOG0927">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_45_1_1"/>
    <dbReference type="InParanoid" id="D5GMQ1"/>
    <dbReference type="OMA" id="WEIKKHI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050334">
        <property type="entry name" value="Molybdenum_import_ModC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43514">
        <property type="entry name" value="ABC TRANSPORTER I FAMILY MEMBER 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43514:SF4">
        <property type="entry name" value="ABC TRANSPORTER I FAMILY MEMBER 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="3">
        <location>
            <begin position="7"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ85794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="548" mass="59892" checksum="AEAC8DAC26F22376" modified="2010-06-15" version="1">MSRHPLISITSTSFRPITSPLTAPILFPNTTFALHPAQKWSILGSSKSTLLSILCGSHIAIPPTGRCYPGLRGTIELVSFTTGSKSTGMLAGANSGETYLSARYEAWREESDVSLRTWLGGSIAGKNPFEEGGEVKGKEALGERKKKVEDVAGTLMLREFLDQSVMTLSNGQSQRARIAQALLKRPEVLAIDEPFMGLDPPSRNLLSELLSRLPRAPQPTQILLGLRPQDPVPAWSTHIAFISNNRILAQGQKEFVISTLRRSGKTILQEGDDTQKPAPTLLDKVWSAPAVPASISQDRITLPSKSTSSPILIQMSNIQITYPPHPPFLQNFSWTIRAGERWGLFGPNGSGKTTLISLLTSDHPQTYALPITHFGHQTRIPGPGRPGVSIFDVQKRIGHASPEVHAFFPKAYTLRRTILSGLAETFRAKPPPATEYAETLLAEFEDIVAGPGGWDDTVFGEACLNVQRLALFLRAVVGKRELLVLDEAFSGMDNAVRDRCFRVLAHGFDHARQALVVVSHVGEEVPEVDMWVRLPDRGSDACAVYGYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>D5GN26</accession>
    <name>D5GN26_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00011059001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430362">
        <property type="protein sequence ID" value="CAZ85919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841728.1">
        <property type="nucleotide sequence ID" value="XM_002841682.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GN26"/>
    <dbReference type="FunCoup" id="D5GN26">
        <property type="interactions" value="443"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GN26"/>
    <dbReference type="GeneID" id="9185845"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011059001"/>
    <dbReference type="eggNOG" id="KOG0777">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014015_6_0_1"/>
    <dbReference type="InParanoid" id="D5GN26"/>
    <dbReference type="OMA" id="ANFAYFW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046165">
        <property type="term" value="P:alcohol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043386">
        <property type="term" value="P:mycotoxin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001">
        <property type="entry name" value="GERANYLGERANYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001:SF44">
        <property type="entry name" value="GERANYLGERANYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ85919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="343" mass="38836" checksum="1651DCBF35930F62" modified="2010-06-15" version="1">MTPEPSTTSRHRHSPTLKPLKPRVDSDAPVQNGTPQTRSADVLVAAGFQRQWSEEKENILLGPYNYLVSHPGKDIRQQSITAFNSWLQVPEVSLQIITKVVAMLHNASLLVDDVEDRSNLRRGFPVAHSIFGVAQTINSANYVYFQALQELLKLNNPLVLRIYTEELLNLHRGQGMDLFWRDTLTTPTEDDYLEMVSNKTGGLFRLAVKLMQAESASDKDYVPLVNIIGLLFQILDDYLNLQSNQYTKNKGFCEDLTEGKFSFPIIHAIRSDPSNRQLLNILGQRTTDEDVKMYAVAYMKDQTGTFDYTRKVIHDLHKKALEAVEEHGGNPLLVAILMKLVAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GN54</accession>
    <name>D5GN54_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00011096001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the ANP1/MMN9/VAN1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430362">
        <property type="protein sequence ID" value="CAZ85947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841756.1">
        <property type="nucleotide sequence ID" value="XM_002841710.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GN54"/>
    <dbReference type="FunCoup" id="D5GN54">
        <property type="interactions" value="88"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GN54"/>
    <dbReference type="GeneID" id="9185873"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011096001"/>
    <dbReference type="eggNOG" id="ENOG502QRPX">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017872_4_0_1"/>
    <dbReference type="InParanoid" id="D5GN54"/>
    <dbReference type="OMA" id="IPKTREG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000136">
        <property type="term" value="C:mannan polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000009">
        <property type="term" value="F:alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000017">
        <property type="entry name" value="Mannan polymerase II complex ANP1 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052086">
        <property type="entry name" value="Mannan_Polymerase_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43083:SF6">
        <property type="entry name" value="MANNAN POLYMERASE COMPLEXES SUBUNIT MNN9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43083">
        <property type="entry name" value="MANNAN POLYMERASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03452">
        <property type="entry name" value="Anp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="37"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ85947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="352" mass="39790" checksum="949FA5356CB2E338" modified="2010-06-15" version="1">MVRPLRRSSPITLLLALVLFLGFIIFILSPSNPVPSIPKSKIASSSSTRTSPNSPPPVVHYYLNNLTTSSDPIGSREKVLVLTPVARFYDEYWQNLLKLSYPRDLIELGFIVPKTREGNTALAKLEDAVRRVQTGSKKHRFLGVTIIRQDFEAPVPQNEKERHALSAQKERRAAMSRARNSLLYTTLGTETAWVLWLDADIIETPHTLIQDLASHDKAVIVPNCYQRYTEKGKEKIRPYDFNSWHDSDTALAMAGKMGPEEIILEGYHEMATYRSLMAYEMAEGGDPHQETELDGVGGTALLVKAEVHRDGAMFPPFPFYNLIETEGFAKMARRLGHQPYGLPNYLVYHYNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNB9</accession>
    <name>D5GNB9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00011213001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430363">
        <property type="protein sequence ID" value="CAZ86012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841821.1">
        <property type="nucleotide sequence ID" value="XM_002841775.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNB9"/>
    <dbReference type="STRING" id="656061.D5GNB9"/>
    <dbReference type="GeneID" id="9182212"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011213001"/>
    <dbReference type="eggNOG" id="ENOG502SBYU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076390_0_0_1"/>
    <dbReference type="InParanoid" id="D5GNB9"/>
    <dbReference type="OMA" id="HPTRELM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41805">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41805:SF1">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN FYV7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ86012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="205" mass="23224" checksum="2166F5E8BAF130D4" modified="2010-06-15" version="1">MGKPSLERPHKKAFSVGPANLPDGTYKRKGRSLIHKAKVKKSLAKTISGNAPDTDPQAIRAKRLLEEAAAERERAKSTEPKDCEDGDAIVATTEMHPERRVALDQPETARVEENPKNVDKGAWRKRGKKKKTSRFLKEEQEAARLREEREEKEREIEARKVAMEKSRKERALRKRVMSARTTKGQQKLGRQSALLLEKVKAQVGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>D5GND5</accession>
    <name>D5GND5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">GSTUM_00011235001</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DEAD box helicase family. DEAH subfamily. PRP16 sub-subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="FN430363">
        <property type="protein sequence ID" value="CAZ86028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841837.1">
        <property type="nucleotide sequence ID" value="XM_002841791.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GND5"/>
    <dbReference type="FunCoup" id="D5GND5">
        <property type="interactions" value="775"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GND5"/>
    <dbReference type="GeneID" id="9182228"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011235001"/>
    <dbReference type="eggNOG" id="KOG0924">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001832_6_1_1"/>
    <dbReference type="InParanoid" id="D5GND5"/>
    <dbReference type="OMA" id="VDVMFHR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034458">
        <property type="term" value="F:3'-5' RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000007">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000615">
        <property type="entry name" value="pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000018">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase prp16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF91">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR ATP-DEPENDENT RNA HELICASE PRP16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="539"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="724"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="82"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="469"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1179"/>
            <end position="1227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="126"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="155"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="197"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="271"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="294"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="491"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1200"/>
            <end position="1212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00070009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAZ86028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1227" mass="138154" checksum="1532C45CAC436DB6" modified="2010-06-15" version="1">MAEDIPPGLNDKYSHHVAIQISRQQNSQTVNDYLARNIVKIARDQPGPKTFILACKGLGRYPDDFLTEIFEEVRIRDRYDQVHGKSGLSESSTFGAPERATDTGSNLPGGLLLARKGDRGGGNGEEGGKKESRLGLDRLAKRIKLDLAADGEPEGDPKEKNGDEKIEFKKPAIPIGGRHIRERRVETPSHGPGLSQEARERLNAHRARRGGEGGRDDRGGDRRGYSRDYYPKDLRDRRDNSRYTPQNLRRQDPTPLRNVDVRYTERYANARPSTSRSSRFGNATPRMPSTFDRDPEDVPEINEEDDSALDRDWYASDELGHAFGDEYHNPFGSADNSWADQQREQALLEKKQGKRMSARAQQKMKDVDAWETNRMLTSGVAQRADYNDDDLDDDEEVRVHLLVHDLRPPFLDGRRVFTKQLDPVPAIRDPQSDMAIFSKKGSLLVKERRQRREREKQAQEATNIAGTALGNVLGIKEDDDGDSAAPTGGGGKEETSGSKFAEHMNKQRPGEGNSEFTKSRTLREQREFLPAFAVREDVLRVIRDNQVVVVVGETGSGKTTQLTQFLYEDGYGKIGMIGCTQPRRVAAMSVAKRVSEEMEVRLGGLVGYAIRFEDCTSDETVIKYMTDGVLLRESLVDPNLDKYSCIIMDEAHERALNTDVLMGLIKKILARRRDLKLIVTSATMNAERFSRFYGGAPEYIIPGRTFPVDVLWSKSPCEDYVDAAVKQVLQIHIGQGVGDILVFMTGQEDIEITCEVIAERLKQLNNPPKLNILPIYSQMPADLQAKIFERGEGGARKVIVATNIAETSLTVEGIMYVVDAGYSKLKVYNPRMGMDALQITPISQANASQRSGRAGRTGPGKAYRLYTEQAFRNEMYLQTIPEIQRTNLSNTVLMLKSLGVKDLLEFDFMDPPPQDTMTTSLFDLWALGALNNVGELTFLGKTMASFPMDPSLSKLIIMSGEYNCGEEMLTIVSMLSVPSVFYRPKERQEESDQAREKFFVAESDHLTLLHVYTQWKSNGYSDRWCIQHFLQPKALRRAKEIRNQLMDIMKFQKMELKSCGTDWDIIRKCICSGYYHQAAKVKGIGEYTNLRTSVTVQLHPTSSLYGLGYLPDYVVYHELILTSKEYMSTVTAVDPHWLAELGGVFYSVKEKGYSARERRTTENEINKRMEIEAKMAEDKARAEEDAANARAAASVKRPMTTMTQKRMGTQTGVVKRPSTPAMRKRGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNE3</accession>
    <name>D5GNE3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00011245001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFIIF alpha subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430363">
        <property type="protein sequence ID" value="CAZ86036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841845.1">
        <property type="nucleotide sequence ID" value="XM_002841799.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNE3"/>
    <dbReference type="FunCoup" id="D5GNE3">
        <property type="interactions" value="137"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GNE3"/>
    <dbReference type="GeneID" id="9182236"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011245001"/>
    <dbReference type="eggNOG" id="KOG2393">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025955_0_0_1"/>
    <dbReference type="InParanoid" id="D5GNE3"/>
    <dbReference type="OMA" id="IPIMTTK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005674">
        <property type="term" value="C:transcription factor TFIIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001096">
        <property type="term" value="F:TFIIF-class transcription factor complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008851">
        <property type="entry name" value="TFIIF-alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011039">
        <property type="entry name" value="TFIIF_interaction"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13011:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIF SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13011">
        <property type="entry name" value="TFIIF-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50916">
        <property type="entry name" value="Rap30/74 interaction domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="344"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="397"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="591"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="151"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="59"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="344"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="400"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="432"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="442"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="474"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="558"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="657"/>
            <end position="691"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ86036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="762" mass="83302" checksum="EF6F99081637EE0A" modified="2010-06-15" version="1">MASPAPSSAPTTGSPVPRMVARRREANSNPLVQRQKRPRAPVVNRQKLAAPPPPPPIAAAPAKTVSSTASSVSGTGSAIAVDDEGEWHEFKLTTTKASLLKGLRHHVMRMQSKREIDPTDPTQFTRPVRLHRRDPRAPLQGAKEEAAANQLAAANKAAEAGEKAKDEVEQAKKDAEKQANLDKIAPFGGAQKQKQKVFHKKTQQVFKSNDAEKKLRYEEFFPWFIEDFDNKNTWQGQLESSLSKGVFAMFVLEEGAFRMVPIEKWYKFQEKNRFPTMTSEEAELEMKKNARPPRWLMKRTEEKESKQRELEENARANKTLFTRKGDRLKGEADNDDLDFEDDQFADDEEAPIMEGEEQENKEIEKRIKKEQLSANFFDARDERDWEKEELERKKLEEVRRKHGKRVKKALMNREGNFTYESDSDANPYGTSGDEDSEDEEETARKEEEKKKEEERKKAEAAAEKEKADPRKSKTPPVATPASTSTPSAVAAPAPSQPKSKATGGTTGAPHPNAANKKDKHPAGTPLPLSDKKPQLKRAGTPPHPESGTESRKKPKKNNAATNSTTGKSTTLPILGGTDKKNIVKLKVASESLSQIASSTPSPSPPPPGVGAGNKKRKADVSAGARSGDERSGEMSDGPGSKKQRIKITTGGRSLSPAAAAAASRQGTPGASRAGSPAAAAGAGNTSAPAARGTPGPPDFITDAEIRTVLSSNPEGVTLKFIADKFKNRVRNEEVKKGLVSSLRRLSVSTDKRTYKLKEGVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNH9</accession>
    <name>D5GNH9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00011297001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430364">
        <property type="protein sequence ID" value="CAZ86072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841881.1">
        <property type="nucleotide sequence ID" value="XM_002841835.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNH9"/>
    <dbReference type="FunCoup" id="D5GNH9">
        <property type="interactions" value="82"/>
    </dbReference>
    <dbReference type="GeneID" id="9186147"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011297001"/>
    <dbReference type="eggNOG" id="ENOG502QRB5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034574_1_0_1"/>
    <dbReference type="InParanoid" id="D5GNH9"/>
    <dbReference type="OMA" id="MNWAFVI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051285">
        <property type="term" value="C:cell cortex of cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009571">
        <property type="entry name" value="SUR7/Rim9-like_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052413">
        <property type="entry name" value="SUR7_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28019">
        <property type="entry name" value="CELL MEMBRANE PROTEIN YLR413W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28019:SF3">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_6G07470)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06687">
        <property type="entry name" value="SUR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="187"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="231"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="290"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ86072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="320" mass="34468" checksum="814ED23817D5EF41" modified="2010-06-15" version="1">MGKAGRFACILTPMLLTLASLCCVVIVMIGGTNKSSSLISGLYFLKIDTRFIDTPENMDLIPGTDFDDELLKSGFNATASDLGLADFYTSSLWNYCSGQSLDDHWEVTECGKPSAGYAFDPIRILDVEAEGKDVDFPDSVKKVSKAVSAASKVMIAMYVLGAVATVVTFAVGWFGLLSRWGSCVTTIFADIAFFFLLTASIISTALYVSIREAFNKALKEFSVNAQINRQMFIITWLSVAFSLGACLFWMLSTCCCSGRRSRVMGTDHKGKGKSSSPAGGIRGGYERIAAPYKGGSGSGMKPMSEVGKKQAAGYEPFRHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNS3</accession>
    <name>D5GNS3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00011407001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430367">
        <property type="protein sequence ID" value="CAZ86139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841948.1">
        <property type="nucleotide sequence ID" value="XM_002841902.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNS3"/>
    <dbReference type="STRING" id="656061.D5GNS3"/>
    <dbReference type="GeneID" id="9188375"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011407001"/>
    <dbReference type="eggNOG" id="ENOG502SDRZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_488346_0_0_1"/>
    <dbReference type="InParanoid" id="D5GNS3"/>
    <dbReference type="OMA" id="FHTAYDE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="297"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="388"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ86139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="496" mass="55285" checksum="796E8A118B7F84FA" modified="2010-06-15" version="1">MYEGSVYEGEDRSDLSWKAERILANAKKKLDLCGQNISRARSSLILSPSTTPTSFTEQLESVAVLPKQLPSLHSQRFKFNNNNSSSHGRAASESAVPRSYSSHNSANGRSNGRSGDGLGLRTIEDVNEHLSEIERPQSEMSRSKSTQQMRVLRDQMKDLRGKITSLQQQTRSDSMKRRSISSLRSTPNPYESHDSVTPTLDNIKQERAHRGNENWRTIDHEDDIPDDYSSRSGTPRPRPFRNSASPFEERHEDREDAFSYDALFYGNGVYAGSNGTITRPMSYSSDGTNSTTTAVMEPLKASPSPPSTMKRSDSHGSIESFETAAEVPSPVPRRPTEPSLPVMASEWLTPTASSPRDDGYHSAPHTPRSPYSTNVAFSKAKPPVITNSLSGSSIYLPPPETQKSQKRKSSIAIGASESIDSVLVTNADNVSEIELKLGEGDRDLIERMIEGLGSFCCKMEMERREDMREEYRRRLECALRVLQGVEVYDDEDDETF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNT9</accession>
    <name>D5GNT9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00011478001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430368">
        <property type="protein sequence ID" value="CAZ86182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNT9"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011478001"/>
    <dbReference type="HOGENOM" id="CLU_1397254_0_0_1"/>
    <dbReference type="InParanoid" id="D5GNT9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="8"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="144"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="161"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ86182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="195" mass="21579" checksum="929DBCC1F85C59CD" modified="2010-06-15" version="1">MGLRLRQKSPSPSRSSQQQEQHRQQQPSPPPTPQPRKSLRKARKNTHRSRSRSPSGLDAAIVLPNNIIPYGVLFQDDEPEDEGYSSPPSTPTDPPPHMQMDGWRMVPERGLAMELMFGNMDADLIEEIIGASSSDDMDIDEPATPSNTRTNTRIPHTHLNSGPSSEASSSSSRDSRGRRYSGNGFGGRVVDENYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNU6</accession>
    <name>D5GNU6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00011512001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430369">
        <property type="protein sequence ID" value="CAZ86189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841998.1">
        <property type="nucleotide sequence ID" value="XM_002841952.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNU6"/>
    <dbReference type="STRING" id="656061.D5GNU6"/>
    <dbReference type="GeneID" id="9182823"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011512001"/>
    <dbReference type="eggNOG" id="KOG1292">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017959_7_1_1"/>
    <dbReference type="InParanoid" id="D5GNU6"/>
    <dbReference type="OMA" id="GLGFDWN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042907">
        <property type="term" value="F:xanthine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006043">
        <property type="entry name" value="NCS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006042">
        <property type="entry name" value="Xan_ur_permease"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00801">
        <property type="entry name" value="ncs2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42810">
        <property type="entry name" value="PURINE PERMEASE C1399.01C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42810:SF2">
        <property type="entry name" value="PURINE PERMEASE C1399.01C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00860">
        <property type="entry name" value="Xan_ur_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="163"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="218"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="249"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="288"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="385"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="413"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ86189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="465" mass="49454" checksum="C93B69D1836B8E37" modified="2010-06-15" version="1">MREPRRPAPFFGLDDQLPVLLAVLLGFQHALSMVAGIVTPPMILGGAGGANLPVAVQQYLVSTSLIVCGILSAIQITRFHIWGTPYVPGANCPGEVGTTFAIIPVAEGALDQMYANGQVRSRYANYDYFLPNLNLGAILGTGCVCALLEIALSFVPPRVLRRIFPPLVTGPAVMLVGVKLIASGFKNWAGGSGPCALMPEKGFFSLCPNTGAPHPLPWGSAQFIGLGFSVFITIIICERFGSPIMQSTSVAIGLLVGCVIAAATGYFSKMNIDLAPAVSFIWVHTFKLSIYGPLVLPTLAVYVILVCEVIGDVTATCDVSKLDVDGKMFDSRIQGGLLADGINGFLASLATMTPMSTYAQNNAWITPLPDHPGVIALTRCANRGAGYGCCFFLILMGIFSKFAASLVAIPPSVLGGMTTFLFCSVAVSGMRIASTMPFTRRSKRFFIQFPPFIPLSLFPRSVHTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5GP17</accession>
    <name>D5GP17_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">U3 small nucleolar RNA-associated protein 22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00011623001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
            <location evidence="1 5">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the NRAP family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430371">
        <property type="protein sequence ID" value="CAZ86260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842069.1">
        <property type="nucleotide sequence ID" value="XM_002842023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GP17"/>
    <dbReference type="FunCoup" id="D5GP17">
        <property type="interactions" value="934"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GP17"/>
    <dbReference type="GeneID" id="9183553"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011623001"/>
    <dbReference type="eggNOG" id="KOG2054">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003502_1_0_1"/>
    <dbReference type="InParanoid" id="D5GP17"/>
    <dbReference type="OMA" id="NPHGGKE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032545">
        <property type="term" value="C:CURI complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034456">
        <property type="term" value="C:UTP-C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006409">
        <property type="term" value="P:tRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005554">
        <property type="entry name" value="NOL6/Upt22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035082">
        <property type="entry name" value="Nrap_D1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035367">
        <property type="entry name" value="Nrap_D2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035368">
        <property type="entry name" value="Nrap_D3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035369">
        <property type="entry name" value="Nrap_D4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035370">
        <property type="entry name" value="Nrap_D5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035371">
        <property type="entry name" value="Nrap_D6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17972:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17972">
        <property type="entry name" value="NUCLEOLAR RNA-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03813">
        <property type="entry name" value="Nrap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17403">
        <property type="entry name" value="Nrap_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17404">
        <property type="entry name" value="Nrap_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17405">
        <property type="entry name" value="Nrap_D4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17406">
        <property type="entry name" value="Nrap_D5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17407">
        <property type="entry name" value="Nrap_D6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Nrap protein" evidence="7">
        <location>
            <begin position="183"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="8">
        <location>
            <begin position="330"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="9">
        <location>
            <begin position="476"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="10">
        <location>
            <begin position="647"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="11">
        <location>
            <begin position="830"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="12">
        <location>
            <begin position="971"/>
            <end position="1097"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="283"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="36"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="283"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF17407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ86260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1101" mass="123774" checksum="3F991D39C878EBBE" modified="2010-06-15" version="1">MAPATKKRRTEIEEGDEEVRAKETIGGLSSEEEMEAVSKEDDFGDRTFEAKSKPRKTPKHSSSEQEKAQAISESYESKTNVFRMETEELLSEIRVDYRKRMAPAEKMLHKLKNIIEKIPRKTGATIDKVEGEMRKCKIHIPFPEPRPGADIKYKFSYERPAYMNIAGSYALKTVAKQHEGFAIDLIIQIPSTVFQEKDYLNYRYFHKRAYYLATIAEAIQDSGLDLQLSYVDLHDDPLRPIIVAAPSGGSETDFTTSKCLIRIIPAIAKNTFSASRLRPDRNAVRPVAERDTAKNSLPPTPRYNSSILADTTYFEHLAQLHDISSEAKVFKDACLLGRTWLRQRGFGSNINQGGFGHAEWSILMSLLLKGGGSKGNSVLAKGYSNYQMFKAMLQFLATRDLIVNPLLFDVESECARWPNGPNVFDGKIGLNILFKMTEWGYRLLKHEAEITLKLLSDPVMDHFEEVFLHRLDDPRFKFDALAYIPIPECLNKNNGLDPLLSFSQKLYDMLSTGLTDRAKLIHLTLPSISDLPVRADGINRSPAPAAQIIVGLLLNAAQCNRAVDHGPPAGHKDGASYRVFWGDKAELRRFKDGSILESVVWGTKDSKQSIYNQIIQYLVAKNFGDGIGNHIKFIGDEYTTAVGGGRDVSLFQPAMAAFEKLIKELRNLEGLPLAIRQVSAAAPVLRYSSVEVPLLSSGQLMQPADVVVQFESSGRWPDDLIAIQKTKTAFLLKMSELLEKAGTVTRIGLENIDYPTMNSAFLEVVYYEGSAFRIRIYHDREQTLLERQLLNKDLPGKKRDEVTAALSEHRRRFIVGPLHTQEVQKLSHRFPALSPSIRLLKRWFQTHLLSREISEELAELMTIRTFVATSSPPNSVMSGFLRTLHFISLWDWRAEPAVIGTVAKNASETFDAIRKSDPAINRHAMVIPTEYDPTGTTFTEFGPRKVVAARVTALARSAIGASFKNMFISSTKDYDFVMYIEPKYTLPGIRSKSSKFKNLQKTEITPQDIAKLGLNVPDTFVDELNDIYGDTVIFFTDGNIIAGLWNPSSESGRRWKVNIPYSTIPQVRGNGEEVLVLNKKSILNEISRLGGEILTKIEIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPM2</accession>
    <name>D5GPM2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00011922001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430376">
        <property type="protein sequence ID" value="CAZ86465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842274.1">
        <property type="nucleotide sequence ID" value="XM_002842228.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPM2"/>
    <dbReference type="GeneID" id="9186705"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011922001"/>
    <dbReference type="HOGENOM" id="CLU_2759656_0_0_1"/>
    <dbReference type="InParanoid" id="D5GPM2"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ86465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="70" mass="8278" checksum="B9E63AEBCCF013A5" modified="2010-06-15" version="1">MGASLIFSPSSWKGHHPQRRYASASCGRHRHLVLRRFQCQNRIRCLPRPHRRRLPLRMSGLFGKCPRQTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5GQ02</accession>
    <name>D5GQ02_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">6-phosphogluconolactonase</fullName>
            <shortName evidence="6">6PGL</shortName>
            <ecNumber evidence="4 6">3.1.1.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00012117001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Hydrolysis of 6-phosphogluconolactone to 6-phosphogluconate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>6-phospho-D-glucono-1,5-lactone + H2O = 6-phospho-D-gluconate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12556"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57955"/>
            <dbReference type="ChEBI" id="CHEBI:58759"/>
            <dbReference type="EC" id="3.1.1.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 6">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. 6-phosphogluconolactonase subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.31" evidence="4 6"/>
    <dbReference type="EMBL" id="FN430380">
        <property type="protein sequence ID" value="CAZ86595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842404.1">
        <property type="nucleotide sequence ID" value="XM_002842358.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GQ02"/>
    <dbReference type="FunCoup" id="D5GQ02">
        <property type="interactions" value="251"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GQ02"/>
    <dbReference type="GeneID" id="9182337"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012117001"/>
    <dbReference type="eggNOG" id="KOG3147">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053947_0_1_1"/>
    <dbReference type="InParanoid" id="D5GQ02"/>
    <dbReference type="OMA" id="YQLFEFE"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00409"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017057">
        <property type="term" value="F:6-phosphogluconolactonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01400">
        <property type="entry name" value="6PGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1360:FF:000005">
        <property type="entry name" value="6-phosphogluconolactonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005900">
        <property type="entry name" value="6-phosphogluconolactonase_DevB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039104">
        <property type="entry name" value="6PGL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006148">
        <property type="entry name" value="Glc/Gal-6P_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01198">
        <property type="entry name" value="pgl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11054">
        <property type="entry name" value="6-PHOSPHOGLUCONOLACTONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11054:SF0">
        <property type="entry name" value="6-PHOSPHOGLUCONOLACTONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01182">
        <property type="entry name" value="Glucosamine_iso"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucosamine/galactosamine-6-phosphate isomerase" evidence="7">
        <location>
            <begin position="12"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ86595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="262" mass="28484" checksum="882C3E6C09AF1027" modified="2010-06-15" version="1">MTGQPSLYAFPDNSTLQKTLYDYILRAQSAALMRHQVFKIAVSGGSLPKLLASPDLLASSDIHWDKWEIFFADERAVPLTHPDSNYALLEQELLSKIPKTGPQPTVHPIDPGKLSDTQDLANAYEESLIRSFAAKDSVKLPVFDLLLLGCGPDGHTCSLFPGHVLLREKTAWVAAIEDSPKPPPERITLTLPVVTHAHKIAFVAAGAGKKEVLRKIFDTHPEAEAGEVLPCALVNLGAGDKVSWFCDQAAVEGVSFPRKSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4C8</accession>
    <name>D5G4C8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00004051001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835250.1">
        <property type="nucleotide sequence ID" value="XM_002835204.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4C8"/>
    <dbReference type="GeneID" id="9184996"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004051001"/>
    <dbReference type="HOGENOM" id="CLU_641219_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4C8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40622">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40622:SF1">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003071963" evidence="3">
        <location>
            <begin position="17"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="351"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="281"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ79371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="428" mass="47695" checksum="ECE5B570414CF675" modified="2010-06-15" version="1">MRYLFAAAPFVLPALGAAVATIDRPLDELLRGFKGSEESYAVRHHKGESFLTARVACNGCRAYAGSKDQNGLKYVPNMGLETDLIFDLRIPNDEPTTLKINDAGVIPFGPNLVVKAYQIPRQTRAEHFLFETAESEWKNLPIGYDLMLQSSREGLESTQKQVIVSFRVTSVGNERNDAIPELEIVVNEELATKRLTMKSVFVKELAGPPLVWLDELEEVKDLIPLLGLPPLLDAPAAFDDTTPVDVDIEIEMEKVPCGTNFRCILDHAIDNLRALKDSVVGTKAPSAPPAPPRRPCNKQNQKEPNLDDEVVSALPIDLDFPEDPHFVDFDSLRTEEKLPLGLSLLNMLSKIVFPVFIGVTAGVAVSLLGLILGQLFMLGWKRIRASRKARRHYKKCKSSCEARAMLPIEEEEDLHEYRDEGIEVVEKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4G9</accession>
    <name>D5G4G9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00004102001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429987">
        <property type="protein sequence ID" value="CAZ79412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842492.1">
        <property type="nucleotide sequence ID" value="XM_002842446.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4G9"/>
    <dbReference type="GeneID" id="9184041"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004102001"/>
    <dbReference type="HOGENOM" id="CLU_570106_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4G9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="338"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="430"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="338"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="437"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="479" mass="51070" checksum="811C9CC558ACCC63" modified="2010-06-15" version="1">MSATNTSPIFGNFQRPFPISQEAATRVALKNLVSLLHPPNATLQNLDATKPQGFQAASCGSGQGQPGDDLRGQRLGPHQPTEIQNIAPDTGSDCDNGNASTLKGVAWPAPEETTLAYHDSSQGREMVAALRMRFDAPTSATAGSESPKPLESPIPRWPGTGKDKSGFFETVEVKTELSEVPLEALQYTERLQNTQSVALVTSHQVVESAIEGLSGSDADASLPTNADGEPDMNDEALERLVAESGLHMDELLSGVMEPEAFTTSESSQPSVAQRVHGDIGDLVGHGSEESMSLDQINALLGYLTENMDLGEPVNTSSPHSTNLVSHDQVADPTQDTIAAPTNNWRNRQYPSTPSANTISRGTKRCSPDDSSSLPKRICKPPQSPIALTQLQAILQSSDHSQPISRHHVSSSYASSFGNSENMNKRLMKPPPYRPASKSPSIGITTGPGSAATIHAKSNEHEKKVKSMGFPPLMAGMKPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4U4</accession>
    <name>D5G4U4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="15">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">GSTUM_00000202001</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN429991">
        <property type="protein sequence ID" value="CAZ79537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835380.1">
        <property type="nucleotide sequence ID" value="XM_002835334.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5G4U4">
        <property type="interactions" value="85"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G4U4"/>
    <dbReference type="GeneID" id="9183019"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000202001"/>
    <dbReference type="eggNOG" id="KOG1012">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001661_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4U4"/>
    <dbReference type="OMA" id="VLMDDYM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061817">
        <property type="term" value="P:endoplasmic reticulum-plasma membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04044">
        <property type="entry name" value="C2A_Tricalbin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04052">
        <property type="entry name" value="C2B_Tricalbin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04045">
        <property type="entry name" value="C2C_Tricalbin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04040">
        <property type="entry name" value="C2D_Tricalbin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21678">
        <property type="entry name" value="SMP_TCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="C2_dom"/>
    </dbReference>
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        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037761">
        <property type="entry name" value="C2A_Tricalbin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037765">
        <property type="entry name" value="C2B_Tricalbin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037762">
        <property type="entry name" value="C2C_Tricalbin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037756">
        <property type="entry name" value="C2D_Tricalbin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056910">
        <property type="entry name" value="TCB1-3_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017147">
        <property type="entry name" value="Tricalbin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052455">
        <property type="entry name" value="Tricalbin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46980">
        <property type="entry name" value="TRICALBIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46980:SF2">
        <property type="entry name" value="TRICALBIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24920">
        <property type="entry name" value="C2_TCB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25669">
        <property type="entry name" value="SMP_MUG190-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037232">
        <property type="entry name" value="Tricalbin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="9" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="178"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="SMP-LTD" evidence="14">
        <location>
            <begin position="228"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="429"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="576"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="712"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="1056"/>
            <end position="1174"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="1339"/>
            <end position="1452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="77"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="872"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1301"/>
            <end position="1352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1464"/>
            <end position="1489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="13"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="83"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1322"/>
            <end position="1344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1464"/>
            <end position="1474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="1475"/>
            <end position="1489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAZ79537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1489" mass="162780" checksum="2DDDBA211FB76A2E" modified="2010-06-15" version="1">MTDRGVAPLKSAAEIEREAKAREHELAQESRKGGATVYEFDPNATPEEKAAQAAKAIPGELGLAKGKVTDITRAVQNSASSHRNNGATNGRNGAQSLPDAPKTPKTPGVVNGQPKAGDVEWSRTGWEPQFGSLNDAFGLDDAVAEDHQTLLESKLDDKFFGDWYHNAGVIGFACLSTWFLTLIGGGLGWVILVMACCATYYRTSIRRVRRNIHDDLTREFAKSRLDTDVESLEWLNSFTVKFWPIYQPVLAVTIINVVDQVLEGATPGFLDSLKLPTFTLGTKPPRIEFVKTYPKTEDDIIEMDWKFSFTPNDTSDLTSRQLRNKVNPKVVLEARVGKGLASKGVPIVVEDMAFSGVMKVKIKLQLAFPHIEKVDVCFLGRPTFDYVLKPLGGETFGIDIGFLPGLNGFIQEMIHTNLGPMFYAPNVFTVEVAKMLGGAPIDTAIGVLVVTIHNAHGLKNPDKFSGTPDPYTVFSINNREEIGKTKVVNEDANPKWNETKYILINNYNDSLTMTVYDWNEFRKDKELGIATFALHKLQDDPEQENIVMPVMVGGKARGQVSCDFRFFPILEGAVLEDGTKEPAPESNTGILRFTVSQAKDLDSSKSLVGFLSPYAIQTLNGKTINRTKTVKRNNNPIWEVSKEILVTNRKTAKLGLQIKDERDLAANPLLGTYMIKLDDLIDSNSKGTEWFNLSGAKTGRVKMTAQWKPVAVKGAPGGTGGYIRPIGVMRVHLQSARDLRNLEALGKSDPYVHVLLSGVEKGRTVTFINDLNPDWNEILYIPVHSPRERLTLEVMDQENMGKDRSLGHLDVNCDQYIKQGEDGLWLEHSEKINRSEGLKLDRGVKGTLNFTVAFYPCLNIADPEGEEAERKLKEGEKNEKYEQSVEQKVEKSAVDIENEKSKAQIEENTLAEAAAGSDSDGDEKKTSRIKLTPKELVKYDSGLLVFKIIEGQFAHKGCYLEVLMDDMLYPAYSTARIKSKDTKFGEIGDAFVRELEFSKVTLRLREHGKDGEQEDEILGKLTGGTLETVKQCLNNPTVLALKDKDGEVSKVTISLNYIPVMMTLDPSESINNMGTLRVDVLDAANLPSADRNGKSDPFCVFALDGKSLYKTDVQKKTLHPSWNEFFETKVSSRTAANLVVEIFDWDLAGKADFLAKGQIDLTQLEPFTPKPIVIKLTGKQGQEGRFGELRLRLLFRSGYVTRSRQGSSTFHGTFATPGKVATGVAGAPLKVGGFAAGGITKGASFLKKSAFGRKAKDTNGVAEEIDREMLAPSSAVSNGGATKGISEGGVAAYDPDGSLVALDGSEDSLDPGQRASGKRTGLSHSPSTAGSPHNRNRSVSSTLSVPGAAPGGADHGTATVRIVAASGFPTNSNVQLRFKTLDKGKELHKSKSVKSSTGELAWEESFSFNCTADQQFKVYAKDNHRLRGDEELGEGLFVVDDTGNGGDTVVPVGQGKVVLRTSFKSSETASSVSPRNRRGGLLKVRSHAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5G543</accession>
    <name>D5G543_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="13">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00000247001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429996">
        <property type="protein sequence ID" value="CAZ79628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835471.1">
        <property type="nucleotide sequence ID" value="XM_002835425.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G543"/>
    <dbReference type="STRING" id="656061.D5G543"/>
    <dbReference type="GeneID" id="9186326"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000247001"/>
    <dbReference type="eggNOG" id="KOG0138">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_8_0_1"/>
    <dbReference type="InParanoid" id="D5G543"/>
    <dbReference type="OMA" id="HMMNLES"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000062">
        <property type="term" value="F:fatty-acyl-CoA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004361">
        <property type="term" value="F:glutaryl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046949">
        <property type="term" value="P:fatty-acyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.540.10:FF:000003">
        <property type="entry name" value="glutaryl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000006">
        <property type="entry name" value="Glutaryl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052033">
        <property type="entry name" value="Glutaryl-CoA_DH_mitochondrial"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42807">
        <property type="entry name" value="GLUTARYL-COA DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42807:SF1">
        <property type="entry name" value="GLUTARYL-COA DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0274">FAD</keyword>
    <keyword evidence="4 9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="12">
        <location>
            <begin position="48"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="11">
        <location>
            <begin position="163"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="10">
        <location>
            <begin position="272"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ79628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="426" mass="46199" checksum="FCE4FDCD26153F07" modified="2010-06-15" version="1">MLRTILRPAHQGALHITTKITTAAATIRKAHSTTFDWQDPLLLTSALTDDEVTISNMAREYCQEKLLPRVLQAYRDEHYDRRILVEMGELGLLGATINGYGCAGVGNVAAGLVTREVERVDSGYRSGMSVQSSLVMGGIDEFGTQEQKERFLPGMAKGKIIGAFGLTEPNHGSDPGSMETVARPHPTKKGVCLLFGSKTWITNSPIADVLMVWAKLDCKIRGFLVERATAAPGTLSTPAIKDKTGLRASITGMIMLDGVEVPEENMFADVDGLRGPFSCLNNARYGIAWGTMGALEDCITRARIYALERKQFKGNPLAKYQLVQKKLADATTDAAFGLAACLHVGRLKDKGLCAPEMISMIKRQNCDRALANARILQEIFGGNAVSDEYGTGRHVANLFVTQTYEGQSDIHSLILGRAITGAQAFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5G577</accession>
    <name>D5G577_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00000344001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429997">
        <property type="protein sequence ID" value="CAZ79670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835513.1">
        <property type="nucleotide sequence ID" value="XM_002835467.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G577"/>
    <dbReference type="GeneID" id="9188097"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000344001"/>
    <dbReference type="HOGENOM" id="CLU_2361259_0_0_1"/>
    <dbReference type="InParanoid" id="D5G577"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ79670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="96" mass="11163" checksum="136939AC3A9766FB" modified="2010-06-15" version="1">MRSKLECYPVSHPYLSIISSALGANHFPHRQPQLPITIPYHKRTSPQYSSRCLASDLFKFISHAEYGCFCYTKKKQNISTLEDISGRENIYNFSFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5B6</accession>
    <name>D5G5B6_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Exocyst complex component SEC15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00004259001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the SEC15 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835552.1">
        <property type="nucleotide sequence ID" value="XM_002835506.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5B6"/>
    <dbReference type="FunCoup" id="D5G5B6">
        <property type="interactions" value="519"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G5B6"/>
    <dbReference type="GeneID" id="9188128"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004259001"/>
    <dbReference type="eggNOG" id="KOG2176">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009437_2_0_1"/>
    <dbReference type="InParanoid" id="D5G5B6"/>
    <dbReference type="OMA" id="FPFHSEQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090522">
        <property type="term" value="P:vesicle tethering involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.670:FF:000002">
        <property type="entry name" value="Exocyst complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.670">
        <property type="entry name" value="Dsl1p vesicle tethering complex, Tip20p subunit, domain D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.30">
        <property type="entry name" value="Exocyst complex subunit Sec15 C-terminal domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007225">
        <property type="entry name" value="EXOC6/Sec15"/>
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        <property type="entry name" value="EXOC6/Sec15_C"/>
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        <property type="entry name" value="EXOC6/Sec15_C_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048359">
        <property type="entry name" value="EXOC6_Sec15_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042044">
        <property type="entry name" value="EXOC6PINT-1/Sec15/Tip20_C_dom2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12702:SF0">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12702">
        <property type="entry name" value="SEC15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20651">
        <property type="entry name" value="EXOC6_Sec15_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04091">
        <property type="entry name" value="Sec15_C"/>
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    </dbReference>
    <dbReference type="PIRSF" id="PIRSF025007">
        <property type="entry name" value="Sec15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 5" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component EXOC6/Sec15 N-terminal" evidence="7">
        <location>
            <begin position="3"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Exocyst complex subunit EXOC6/Sec15 C-terminal" evidence="6">
        <location>
            <begin position="354"/>
            <end position="694"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR025007"/>
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    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ79709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="712" mass="82108" checksum="3BC48BB80B703BCD" modified="2010-06-15" version="1">MDELDRFSSDRESEIERLCNNNHQDFVSSVNQLLNVRKGTVDLTEEILKLNQSIQKSTDKLVEQKKALVDSRDVRQNIDEATQALRLCLEVLGLANRVGDLLKQKKHYAALRTLDELQNVHLKEVMQYDVADMIQKSVPAMQGMVKEAVMTDLNSWLYRIRESSVLLGQVAFDQTELRRRRQKERIEKSPYLRSFKLNSAIELVLDEREEFDVLDNENVNINFTPLFECLHIHEALGERDEFRVTYANVRRQQKELLLSGSLTLSNEDISSLNKLLEDICGFAIIERATMKKTMSFRSAVDVDELWDSMCKKAISIITPALDNITQADALLRIKNVLALFIQTMDSWDYSVEALDGFLLVLFAKYSQRLKTEFSADFKEIVTSDDYMPMPINSLQEYDNVVNVSWYKPDKERDQLEFPIVLPFSQMYPLCCIDIRNFLNKYYFFSDEYFTHQSAIDEELKSSLDELLCGQVCTSLVERLTSKYLGQIVQILINLEQFEYACHELEILLAEARQSNHGGSTSLKATERFRSEKKTAEKRIFELVNSKIDDLVETAEYDWMATKKLEEPSEYLQQMTLWMRNIMSSTLLGLPKDIKGFIYFDALSHIATSILALPMSDSVKKINKNAVAALDMDIRYLMEFVDSLNEPLLPTIFEELRQTIDLLQSDNAEEFYSIDTRMRRYASVNPINGPVLLEKLVSGAAPVSRLARFRQGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5G2</accession>
    <name>D5G5G2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Double-strand break repair protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">GSTUM_00004321001</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Core component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The MRN complex is involved in the repair of DNA double-strand breaks (DSBs) via homologous recombination (HR), an error-free mechanism which primarily occurs during S and G2 phases. The complex (1) mediates the end resection of damaged DNA, which generates proper single-stranded DNA, a key initial steps in HR, and is (2) required for the recruitment of other repair factors and efficient activation of ATM and ATR upon DNA damage. Within the MRN complex, MRE11 possesses both single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity. MRE11 first endonucleolytically cleaves the 5' strand at DNA DSB ends to prevent non-homologous end joining (NHEJ) and licence HR. It then generates a single-stranded DNA gap via 3' to 5' exonucleolytic degradation, which is required for single-strand invasion and recombination.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 17">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="16">Component of the MRN complex composed of two heterodimers RAD50 and MRE11 associated with a single NBS1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 17">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 17 19">Belongs to the MRE11/RAD32 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835598.1">
        <property type="nucleotide sequence ID" value="XM_002835552.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5G2"/>
    <dbReference type="FunCoup" id="D5G5G2">
        <property type="interactions" value="986"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G5G2"/>
    <dbReference type="GeneID" id="9183741"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004321001"/>
    <dbReference type="eggNOG" id="KOG2310">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009535_1_1_1"/>
    <dbReference type="InParanoid" id="D5G5G2"/>
    <dbReference type="OMA" id="ESCMFNA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140445">
        <property type="term" value="C:chromosome, telomeric repeat region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030870">
        <property type="term" value="C:Mre11 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008296">
        <property type="term" value="F:3'-5'-DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:AMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003691">
        <property type="term" value="F:double-stranded telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051880">
        <property type="term" value="F:G-quadruplex DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000014">
        <property type="term" value="F:single-stranded DNA endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043047">
        <property type="term" value="F:single-stranded telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030437">
        <property type="term" value="P:ascospore formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010791">
        <property type="term" value="P:DNA double-strand break processing involved in repair via synthesis-dependent strand annealing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990918">
        <property type="term" value="P:double-strand break repair involved in meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035753">
        <property type="term" value="P:maintenance of DNA trinucleotide repeats"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010780">
        <property type="term" value="P:meiotic DNA double-strand break formation involved in reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097552">
        <property type="term" value="P:mitochondrial double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007095">
        <property type="term" value="P:mitotic G2 DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062176">
        <property type="term" value="P:R-loop processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00840">
        <property type="entry name" value="MPP_Mre11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000011">
        <property type="entry name" value="Double-strand break repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.110">
        <property type="entry name" value="Mre11, capping domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003701">
        <property type="entry name" value="Mre11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038487">
        <property type="entry name" value="Mre11_capping_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007281">
        <property type="entry name" value="Mre11_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041796">
        <property type="entry name" value="Mre11_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00583">
        <property type="entry name" value="mre11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10139">
        <property type="entry name" value="DOUBLE-STRAND BREAK REPAIR PROTEIN MRE11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10139:SF1">
        <property type="entry name" value="DOUBLE-STRAND BREAK REPAIR PROTEIN MRE11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04152">
        <property type="entry name" value="Mre11_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000882">
        <property type="entry name" value="DSB_repair_MRE11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01347">
        <property type="entry name" value="Mre11_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9 17 19" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12 17 19" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8 17 19" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="11 17 19" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="10 17 19" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13 17 19" id="KW-0464">Manganese</keyword>
    <keyword evidence="15 17 19" id="KW-0469">Meiosis</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 17 19" id="KW-0540">Nuclease</keyword>
    <keyword evidence="14 17 19" id="KW-0539">Nucleus</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mre11 DNA-binding" evidence="21">
        <location>
            <begin position="290"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="531"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="666"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="539"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="566"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="578"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="596"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="621"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="681"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="717"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="743"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="760"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="18">
        <location>
            <position position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00064981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR000882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR000882-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU003447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM01347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="CAZ79755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="769" mass="85985" checksum="233C448D33D66188" modified="2010-06-15" version="1">MPPRSNANTLRILIATDSHVGYNERDPIRGDDSWKTFDEVMSLAKDRDVDMVLLSGDLFHDNKPSRKAMYQVMKSLRANCYGEKPCEIEILSDTSQTFQSAGGHVNYEDPDINVAIPVFSIHGNHDDPSGEGRLCALDLLSVAGLLNYFGRTPENDNITVTPVLLQKGSTKLALYGLSNVRDERLFRTFRDGKVKFLRPDVQQKEWFNLMCVHQNHHGHTETGYLPENFLQEFLDMVIWGHEHECLIEPKFNPEMGFHVIQPGSSIATSLCEGEAVTKHVGILSITGRGFELEKIRLKTVRPFVMKEIVLAEEPQMKNVWKKNTNRTAVTSFLCNTVEDLIEEAVTEWLNAQEDPEVERKDAPLPLIRLRVEYSAPEGGRFETENPQRFSNRFVGKVANVNDVIQFYRKKSAARRKVGGMLEDSGSHVQDSLLEEYGGSIDNVKVEKLVQEFLTNATLEILPSNGLGDAVGQFVDKDDKHAVEMFVEDSLKSYLTKLREIDDLNEETISEAVEQQKSYLEQLFAKGLVKPKRTRKKLKPKPDHWYSDLDGHWSDQPAALVRSGSDNDGDQNDDDGDDSAAPTRKTAARGGRGRGRGSAAASTRGRKAAPAAKGRGRKQVVEDDDDDDVEMIEDDEDEPSIPPPKTTYYPITPFPLIKYIVLTFSSRGRKTTAAPPARKPAAKPAAAPRRPAGATRAANQQASQASTARTRQAKLNFGATQNTQARESQASNSLFHRAIHPPSDDEDIDDDEDDDFEPLPSQRTTRSSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5N3</accession>
    <name>D5G5N3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00001499001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430000">
        <property type="protein sequence ID" value="CAZ79826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835669.1">
        <property type="nucleotide sequence ID" value="XM_002835623.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5N3"/>
    <dbReference type="STRING" id="656061.D5G5N3"/>
    <dbReference type="GeneID" id="9187503"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001499001"/>
    <dbReference type="eggNOG" id="ENOG502RZ22">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012644_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5N3"/>
    <dbReference type="OMA" id="SHEYQDY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054464">
        <property type="entry name" value="ULD_fung"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22893">
        <property type="entry name" value="ULD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="3">
        <location>
            <begin position="560"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="384"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="613"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="442"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="60"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="90"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="101"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="136"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="185"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="288"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="384"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="402"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="616"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="720"/>
            <end position="732"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ79826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="749" mass="81314" checksum="72BFCBA2611A3A1C" modified="2010-06-15" version="1">MSEEEGSSGVNVPYGHHPQQALPFYPQHPNSHHSQHPRTDYSDSFQDWEDLHGEELGPSDSASRPRTANRQPEQHPPHESRRSRRHSPPHHPGSIPPPPHHSSHNRHHTRQPAPSAPHTLSDSDSEDLHYPRQPQSAGPSSPWGSGPSGSPTYPPASGPYHGYDREQPPPPQPRGYQNPLALVPATPPAHPGHPGHLPGPNPLPGGSNPLVAFEHNGYPPHHQDPYSRHPAQSHPGHYPGHSPPHPAYPGQPQHSAYPPGHGSHGSRRSSSMPSYNQYGQPHGAGYHTSHHPQHGHSGHPGHSGHSGHPGHPGHPQHPTHGQPPVSNALTRHQPDPYGYYSNSPYSPYGPNPYGGGYFTSSAHPHHSMGQMTMNSMMVMGPQGYPIPYPMPTQRSPSPPPPVDDKPPEKTEEEKREEERWARLQEERKFRQEMMEEERRWHQEKLDRERKAEEDARARMEAEAAAEAAAKAEAAAKKVAEEAARAEAEAKARAEAEAASKLAAEIAAAAEKAREEATKEAEEKAAAAIAAERAKAEADAKAKAKIKPKPVKFRDAVGSGGMEELIKQAFLHVEVIGQHVIDGHYDLHGPDGEIILPQVWESVIEPDWSITMHMWPMPEPEPEPPAPPPPPPAPVEPEPAPAPEPTPVRAPTPPPPPPPPPPPPAEPEPAPALAPAPPPAPPPPPAVEVVVVEPAPEIREAAPEPAYHHPPPLPQVLAVAPEPPRPKPVPSIPPLLMWTAGRYGSKKGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5T3</accession>
    <name>D5G5T3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00001469001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430001">
        <property type="protein sequence ID" value="CAZ79876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835719.1">
        <property type="nucleotide sequence ID" value="XM_002835673.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5T3"/>
    <dbReference type="FunCoup" id="D5G5T3">
        <property type="interactions" value="516"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G5T3"/>
    <dbReference type="GeneID" id="9187554"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001469001"/>
    <dbReference type="HOGENOM" id="CLU_218497_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5T3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015157">
        <property type="entry name" value="TMA7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09072">
        <property type="entry name" value="TMA7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ79876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="43" mass="4785" checksum="9099F0FBF4E6B803" modified="2010-06-15" version="1">MRRIRFAFKEKQKREAAELKALAEKARGKGPLATGGIKKSGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5G690</accession>
    <name>D5G690_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00001659001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430008">
        <property type="protein sequence ID" value="CAZ80033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835876.1">
        <property type="nucleotide sequence ID" value="XM_002835830.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G690"/>
    <dbReference type="GeneID" id="9182551"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001659001"/>
    <dbReference type="HOGENOM" id="CLU_2639886_0_0_1"/>
    <dbReference type="InParanoid" id="D5G690"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="77" mass="8520" checksum="BCD070F2F6E371B9" modified="2010-06-15" version="1">MASTSSNIAGQSEPCRTQPTERYLSLSFPSSALLGTWRTLTLLLDIGTLIGLPVPLSLHLFRSSYPQELYRSSYQLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6C6</accession>
    <name>D5G6C6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00004423001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. SKI2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430009">
        <property type="protein sequence ID" value="CAZ80069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835912.1">
        <property type="nucleotide sequence ID" value="XM_002835866.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5G6C6">
        <property type="interactions" value="590"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G6C6"/>
    <dbReference type="GeneID" id="9187440"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004423001"/>
    <dbReference type="eggNOG" id="KOG0952">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000335_2_0_1"/>
    <dbReference type="InParanoid" id="D5G6C6"/>
    <dbReference type="OMA" id="MCSATEF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18022">
        <property type="entry name" value="DEXHc_ASCC3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000198">
        <property type="entry name" value="Activating signal cointegrator 1 complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.10:FF:000002">
        <property type="entry name" value="Activating signal cointegrator 1 complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000231">
        <property type="entry name" value="Activating signal cointegrator 1 complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000004">
        <property type="entry name" value="RNA helicase, activating signal cointegrator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000012">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000024">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.10:FF:000001">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000062">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.10">
        <property type="entry name" value="Sec63 N-terminal domain-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050474">
        <property type="entry name" value="Hel308_SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004179">
        <property type="entry name" value="Sec63-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057842">
        <property type="entry name" value="WH_MER3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961:SF4">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1 COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961">
        <property type="entry name" value="DNA POLYMERASE THETA, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05260)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02889">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23445">
        <property type="entry name" value="WHD_SNRNP200"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039073">
        <property type="entry name" value="BRR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00973">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158702">
        <property type="entry name" value="Sec63 N-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="6">
        <location>
            <begin position="280"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="480"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="6">
        <location>
            <begin position="1123"/>
            <end position="1298"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="1328"/>
            <end position="1522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ80069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1952" mass="219576" checksum="BACA3827573EB153" modified="2010-06-15" version="1">MSALPSPAFQWLRHLSRMDDALAHLLAFPGSHLPSSTEYGLDLPPHEEQVESLRTADDLFGELSRDSAPYSLSTGEAAHPPIHDHSRLLAKCNAIPTHRRSGMTAVDLGEHLLNQELQSRLVDLLGLDELEFVMDLMVHRTELAPFGLPSPSQKPLSAPDSALRLLTREERNQALRQSELEHKSLPLGPKLSQPVENYPHVYRAHDAGNTLSSWGKKYSLPVGSQTTDHKEYEEISIPATKVGTVRAGEKLVQIEDMDILCRNTFRNYKSLNRMQSLVYPVAYQKNENMLICAPTGAGKTDAALLTILHTISMDCTPSPGVTSGDAPIYCDKDQFKIVYVAPMKALAAEIVEKLGKRLRWLGIEVRELTGDMHLTKAEIVRTQIIVTTPEKWDVVTRKSTGDTELSLVARTQRQVESTQSMIRIVGLSATLPNFVDVALFLKVNLNQGLFYFDASFRPVPLEQHFLGVKGKPGTKLSNENLDKTSFEKVKEMLQRNHQVMVFVHSRKDTLKTARLFYQMAVDEMCLELFDNTGHDQYNQACKEIARSKGRELRELFKKGFGIHHAGMPRSDRNLMEKMFGDGLIKVLCCTATLAWGVNLPAAAVIIKGTQVYNAQKGQFMDLGILDVLQIFGRAGRPQFQQYGIGFICTSHDKLAHYLSAVTQQHPIESRFTERIVDNLNAEVALGTVTSIEEGIQWLGYSYMFVRWKKNPLVYGIGWQELSDDPHLYSQRKQLIVKAARRLQQTQMIIFDEKTQSLTPKNIGRIASDFYILNNSVEIFNNMMKSQATEADILTMLSLSGEFDNIQSRDTESQELENLRKTVCPCQVGGAKGSPHAKVNILLQSYISKANLDDFALVSDSAYVAQNSARICRALFHIALNRRWGNLCQILLSICKSIEKKIWSYQHPLAQFRLPPHIVQKLEELESTGSIEVLRDMDPAEASLSTQIGDLVHNQKMGPQISSLLAIFPTLSVDTEIAPLNRDVLRIHLYITPDFRWDDRIHGNSESYWIWAENSETFEIYHSEFFILNRRKLHDDHELNFTIPLSDPLPPQIYIRVVSDRWLGAETVTPISFQHLIRPDTEAIYTPLLNLQPLPITALRDERLETIYGKKFQYFNPMQTQIFHCLYHTPSNVLLGSPTGSGKTIACELAMWWAFRENPGSKVVYIAPMKALVRERVKDWNARLTAPMSLKLVELTGDNTPDTRTIHDADIIITTPEKWDGISRSWQTRNYVRQVSLVIIDEIHLLGGDRGPILEIIVSRMNYIASQTDRPVRIMGLSTACANASDLSNWLGVKDGLYNFRHSVRPVPLEIYIDGFPERRGFCPLMQSMNRPAFLAIKTHSPRKPVIIFVASRRQTRLTAKDIIAFCGLEDNPRQFLHISEEDLRGLLSQVKDQSLREALQFGIGLHHAGLIESDRQLVEELFTYNRIQVLVATSTLAWGVNLPAHLVIVKGTQFFDAKTEGYKDMDLTDVLQMLGRAGRPQFDTSGIARIFTQDAKKSFYKYFLHTGFPVESSLHKVVDDHLGAEVSSGTISKKQDALDYLTWTFFFRRLHKNPTYYGLEIPPEEQDSLSAMEEANRYLVEMVDNSVKELSKSECVIAYPNGDLESTPLGKIASYYYLSHKTIRNLVRHAKRQATLEDCLGWVCTATEYDELPVRHNEDLINVELSKALPFRAEKLGLPMWDPHIKAFLLVQAFISRIELPISDYITDQNSPFVFMANLKQASIDVLAELGYLSTTSAMITLMQSIKQARWPGDGALSFLPGIEPSIEKSLRENDTVAPLLPKDLASLSQLPAKDLRKLADRAGIPQAGRQFLLAASSIPQISISATTATTSTLSATLTRQNAPQSRDFRIYAPKFPKPQTEGWFVLLGDFRKDELHALKRVGWLTRGGAGGGKPSVKFSLGLPEGCVLEGLDGTLVVMSDGYLGLMQRTGVHLQTKQVVPGTWIEKEGGDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6P9</accession>
    <name>D5G6P9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="15">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">GSTUM_00002159001</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Mel28</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRN7/TAF1B family.</text>
    </comment>
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        <property type="protein sequence ID" value="CAZ80192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_002835989.1"/>
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    <dbReference type="eggNOG" id="KOG0003">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="InParanoid" id="D5G6P9"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:RNA polymerase I core factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="F:RNA polymerase I core promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:nucleolar large rRNA transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048538">
        <property type="entry name" value="Rrn7_cyclin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048540">
        <property type="entry name" value="Rrn7_cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033599">
        <property type="entry name" value="TAF1B/Rrn7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021752">
        <property type="entry name" value="TF_Rrn7_Zf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31576">
        <property type="entry name" value="TATA BOX-BINDING PROTEIN-ASSOCIATED FACTOR RNA POLYMERASE I SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31576:SF2">
        <property type="entry name" value="TATA BOX-BINDING PROTEIN-ASSOCIATED FACTOR RNA POLYMERASE I SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20645">
        <property type="entry name" value="Rrn7_cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20644">
        <property type="entry name" value="Rrn7_cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11781">
        <property type="entry name" value="Zn_ribbon_RRN7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="206"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="294"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="RRN7-type" evidence="12">
        <location>
            <begin position="8"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="Rrn7/TAF1B N-terminal cyclin" evidence="13">
        <location>
            <begin position="81"/>
            <end position="249"/>
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    <feature type="domain" description="Rrn7/TAF1B C-terminal cyclin" evidence="14">
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            <end position="462"/>
        </location>
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        <location>
            <begin position="120"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="411"/>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="10">
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            <begin position="528"/>
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            <begin position="127"/>
            <end position="137"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="10">
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            <dbReference type="ARBA" id="ARBA00006899"/>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF11781"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF20644"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF20645"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAZ80192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="635" mass="71704" checksum="0A5482C293FA0028" modified="2010-06-15" version="1">MEYRRLSRCGVDGCKQTLFYLDDGQWFCKNGHLREGELEVAADDEGFGTQGSRAAEATQEREKKGKVLRGRKGFELFLQCYQLILRKQVHWLVSEKRFPRELEIVVRDLWALQLQKVSTTVPTTADDPTKSRDRYDGGDEYDDGDDGRNLDGSGDVGSSSAGYSSQSSYLYTSDDSQSTAQSRNSQGTQRRRRKRRVPRAADRPKLVGSVGICYLGLIVLGVAVSLGDIHRWVEEQGIVYLRAINEIPSEMKQRLDAEYYVALDPRVCCLLPLSLTGVRPTLGLLHSTVQGLVIMYQVTFGMAFPPLNITLLVFGFMKALGLPLEIYYGFKRLANLISLPFMWNGLRYRGRVTNWPEARLMALLIISTKLLYGLDGVKRTPKSAVEPAATGLKWRAWDGYLRTSNAEHRGLLSGNVTPGSEGGERGRGVGGKDLEVDVEEKDVFDMTGEELDRYMDWFEKNWADDSNTPLTKQIIDLFPTGRAAAERAGVHQAYDGPRSGAPADKPAQHDLTDERLRELNSQFEIYPAQQQPEEEEGEEDEEQQQQGEGDNYSSPQPQPQRGELVTRPGGQYLWHSIPTELPVEQQTLLQAGADLLAITRDDLARIIKVLETQFRDLVKNQRRKEMRERRRMDVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6V4</accession>
    <name>D5G6V4_TUBMM</name>
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        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00002292001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_002836044.1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032065">
        <property type="term" value="P:maintenance of protein location in cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR053005">
        <property type="entry name" value="Nuclear_Pos-Cytoskel_Interact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024774">
        <property type="entry name" value="PH_dom-Mcp5-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28190:SF2">
        <property type="entry name" value="MIGRATION PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G07730)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28190">
        <property type="entry name" value="NUCLEAR MIGRATION PROTEIN NUM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12814">
        <property type="entry name" value="Mcp5_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pleckstrin homology" evidence="3">
        <location>
            <begin position="972"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="21"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="243"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="313"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="519"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="647"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="806"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1127"/>
            <end position="1250"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="118"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="403"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="318"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="355"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="585"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="719"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="822"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="858"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="952"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="1141"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1231"/>
            <end position="1250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        <source>
            <dbReference type="Pfam" id="PF12814"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAZ80247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1380" mass="151367" checksum="EB5C2A6EE3CCF643" modified="2010-06-15" version="1">MDDRFLSGGALSEMDADYTDYATEAPLPSPAGTPYRTPLLSRHPSRHATPSPSPSPSPYPLSSSPPTTNGRASPLRRMSMSHHHDTFDDTISILDPRRFTPTLHANLVAEILSLRRDLESKANLIDTLEHDLSAARSEHETATTSAAMAQREARDVKRQLARVENDDAVGAVARERDEAIEANAELKRQMERQAKKARSLEEELERVETIARRDIERLEDINRVQERRVHVAEGRLKTILGEISAANAMPPPPPLPMPQSESENEDPAPSEVAMSDTASIRTSIQERPSSLVMGDDVAERLGIRFSILTGSQGLSLADELDLDDGEEGDEEYEGHEEEDDDDDNRTETEAEAAGNNDSGCSSGSETGDEGDIQPMREYCEAGVQVEEEEESGEEMERPEIVLLEEARTELGRTHSELDRKSSELEKTCIELDKAQERIGELEEQVDGLAEKVAEKKRALADKEIDHGHKIKVFEARTREVEERWASIESKERALEKMEKSLDERTKNLDKKERMLDNKEAIVTPIPDSPVEKELPILPLDDDNVPAENDAHSPTLSPPVMYETIGTQTDPMKPPALQIIPTIAVTPPPPTPPLPGRPKVPMRNAAVQTRNLRQKTRGMQTDEPAFEDRIMKLPARLMPAAILANNNSALSVASPTQPEQPPPSPPKKSSKRNIKRIPTMAPALAPVPTSAPRSVPDSPGPPGTALIYSSPKAPSEFGETSEKDKDSVRRHLFNGIDLSHDLPQSSGDDDFHDDADLSGNEFKTALSAPKPKKRGDRISGLGRPSPVIEGTDGIIYEAYKKPTNIRRSAMVSSGVHAHHRTRSPSLTSNRTSESGGPKIGPPFPVPIRHSSRKVGGYVSSRTESPTPMSASLPRRALPPGRPTRQQSIRKVRSAAALPSLRTSNSRTRSPPPLSASSAAPDSPLLPPPLPRDEVLAPAFDKQKIGSSKHKHQASTNTSNTGNASIGSSIQQISVVDAIAQTMVGEWMWKYVRRRRSFGVPDSPQAWDGRDESTTGGGQRHKRWVWLAPYERAVMWSSRQPTSGNALLGKSGRKLPIQSVLDVKDDTPLPKGANPAQPLFNRSILILTPARALKFTAPNKERHYVWLTALSFLSHSAHESEGLLALPPPMPFEYDQMQQQRPQSPPLTRPPIPPVPVNRDPPFNPIRDSIRVAKGKGRSRTSRSEVNGYAGSNSLGRNSRRDVDRHGLMTTDSIAEPPSIPRFPGHSRKRSNTARPSTNRSISHGYNPSLHSSFGGSSNGDYYPPGSMGVGGIGLISGNSSLSHGIPGPSLGGWESGSIGTVRMEAFIDPKPKFEFDEEDEYLGNKPSSYRLRRDRRSSRQESYWTRGGDFYAGGSEAGKGSVIGGSIDDDFFRGDDPFREF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6X1</accession>
    <name>D5G6X1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00002366001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430019">
        <property type="protein sequence ID" value="CAZ80264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842529.1">
        <property type="nucleotide sequence ID" value="XM_002842483.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6X1"/>
    <dbReference type="STRING" id="656061.D5G6X1"/>
    <dbReference type="GeneID" id="9184078"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002366001"/>
    <dbReference type="HOGENOM" id="CLU_754769_0_0_1"/>
    <dbReference type="InParanoid" id="D5G6X1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031261">
        <property type="term" value="C:DNA replication preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013948">
        <property type="entry name" value="DNA_replication_reg_Sld3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042511">
        <property type="entry name" value="Sld3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28067">
        <property type="entry name" value="DNA REPLICATION REGULATOR SLD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28067:SF1">
        <property type="entry name" value="DNA REPLICATION REGULATOR SLD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08639">
        <property type="entry name" value="Sld3_STD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA replication regulator Sld3 C-terminal" evidence="2">
        <location>
            <begin position="228"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ80264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="367" mass="40577" checksum="82201A128BCBE3A1" modified="2010-06-15" version="1">MEPSTLDVTPSSNSVPAPSSSRKRKREVEKVVLGDSFVIGPYSSSVFDSSLKLTPAVALPRSFVPLQWLAGTPSRLFSFTSNLPVLGVPGNILVAKVDGERQLAAVEKVQDGVYAIFKLSTQLKMRDVRKTVALARKAPHEPPNLEFDGVGGGRLGSEWWSHLRISDCTVKDLLSSPDPVGTLTMGSPDTEPLRGDQAALQAPTRVPVPPSPGVEALTEESAPPTLSDILENTRKQYFKTLYIAKTSLAYFAKSTLSRARVAMRADASELPAGPGGELIIFLQSMLLPFEKMDIKFRSIIPKIALEEKVQENEVLRAGEEEYVQEWRSASFQDKLVRASDQWLKRRVEELKIREYAYARVRSLFFVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5G795</accession>
    <name>D5G795_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">General negative regulator of transcription subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">GSTUM_00002521001</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Acts as a component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430022">
        <property type="protein sequence ID" value="CAZ80388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836197.1">
        <property type="nucleotide sequence ID" value="XM_002836151.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5G795">
        <property type="interactions" value="1173"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G795"/>
    <dbReference type="GeneID" id="9185286"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002521001"/>
    <dbReference type="eggNOG" id="KOG1831">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000286_3_1_1"/>
    <dbReference type="InParanoid" id="D5G795"/>
    <dbReference type="OMA" id="IDEYHCY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20710">
        <property type="entry name" value="NOT1_connector"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000012">
        <property type="entry name" value="Ccr4-Not transcription complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.840">
        <property type="entry name" value="CCR4-NOT transcription complex subunit 1 TTP binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007196">
        <property type="entry name" value="CCR4-Not_Not1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055454">
        <property type="entry name" value="CNOT1-like_NOT1_connector"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032191">
        <property type="entry name" value="CNOT1_CAF1_bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024557">
        <property type="entry name" value="CNOT1_dom_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032194">
        <property type="entry name" value="CNOT1_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032193">
        <property type="entry name" value="CNOT1_TTP_bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038535">
        <property type="entry name" value="CNOT1_TTP_bind_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040398">
        <property type="entry name" value="Not1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13162">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13162:SF8">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25097">
        <property type="entry name" value="ARM_Cnot1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16415">
        <property type="entry name" value="CNOT1_CAF1_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16418">
        <property type="entry name" value="CNOT1_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16417">
        <property type="entry name" value="CNOT1_TTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12842">
        <property type="entry name" value="DUF3819"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04054">
        <property type="entry name" value="Not1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 HEAT repeat" evidence="13">
        <location>
            <begin position="422"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 TTP binding" evidence="12">
        <location>
            <begin position="608"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 CAF1-binding" evidence="11">
        <location>
            <begin position="824"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1" evidence="10">
        <location>
            <begin position="1089"/>
            <end position="1232"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1-like NOT1 connector" evidence="14">
        <location>
            <begin position="1395"/>
            <end position="1499"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-Not complex component Not1 C-terminal" evidence="9">
        <location>
            <begin position="1679"/>
            <end position="2047"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="772"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1279"/>
            <end position="1330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="793"/>
            <end position="803"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00059181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00074459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF12842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF16417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF16418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF25097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAZ80388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="2058" mass="233730" checksum="E01E2A144907076F" modified="2010-06-15" version="1">MEINNRTMRKLISNNYSYIMSSTSSRSSPNPNHRLLTETIKEIASSPQDAKEKFADVIAGMANDGEMARDFDLAVFLSHFGLDSLQKCILAVGLKQSQRHDLRNKAYEIIHKSFRSMLEILANPSRHPELKPEGIAFFIDQILGEPVYPFADNLKRLEISYAARSRYKNMQLPPEILHVTESIEKLRQQESLVHIFSVLGPESTSSLENCREVLKRKWKVQISESEMTDMLVLMATARNPIEWHADFFVRALQHENLPQSFNWERVVEGLDREDFVLEGTEGLHVILTALQHGNTDPDFPIHKLWGGRWKHPRSQWSVLKGYIKADNLDVKRIPGIRKVFSSADFLSSNSSLKLMVATFETHKLISLDAVDALFHLALHESIPPDVKQAAQAELDKAAKFTPELLLCGALMIPKPWPENLEHVINNLFNMFFEGHTSHQLVFWRLWHVDRPFIIGQFVDYHLGNNLNITRILDIAQELRCLSDLLEVKNAPFILDVGALAARREYLNLEKWLQEMINKHGSDFVTETYRFLRVKAEAEYIQTREGGKPTMVSLRVGPVHTFLTLLDRNHPPMTQDMQEKVTETQRLCIQAYPRLINLGCGMDSIILANNSESNTFPPIVDKEMQQNYKMMYSQETEISEMVTYLRQLKTSSIPQDQDLFACMIHGLFDEYHCYPSYPLQALATTSVLFGSIIVYNLIDDIPLRVALAMVWQAVRDHDPSSTMYKFGLQALVQFKERLKEWKSYCGLLAQVPGLQGTDIWKIAQDVIGGNADRQNELSANGNTDRKEPGAVLTNGDSNASSPESPQHPPFRSLDPTMPFRDPSAYTEPDEEVQDRVLFIVNNVSHSNLEEKLESLKEHLREEHHQWFADYLVVERAKLEPNYHKLYLDLLDKYNDKGLTAEVLRETYVNVIKLLNAEATLNSSNERTHLKNLACWLGGLTIAKDKPIKFKNISFRDLLIEGFVTERLTVVLPFTCKVLEQSTKSTVFKPPNPWLMATIRILVELYQHVELKLNLKFEIEVLCKNLDLDIKTIEPSTDIRETRDRPAEKVEESLQVVEDLPLQPQDFSPTSDPVPAGFADHVIVSSAVQHPAIKRILQLAIDRAIREIIGPVVERSVTIASISTSQLIQKDFATEGDEGKMRNAAHIVVQHLAGSLALVTCKDPLRMSMQNNIRALLAQSGYNEQVISDQTITVCVNDNIDMACQIIEKAAQERAIPDIDDGLSQAYQLRKRHRELRTNRPFVSPDVSQVALQLPDQFRLKPGGLSPQQLSMYEDFNRMARAPNPEGARNSFESAFPNDFSPSGSTIVEPPQAQPRASERSPGASDAGQKEIQSKISVKCREQHLKDLHAEHPLFQFIEAILSNASLAQPNMPRLKDAICTTVASSVCLILYTDTQNQMEVEVLGFLLQKVCELSPLTAKDVVMWLSQGQDDERMFNVPVTVMLLKTQLFALTHLDATIARSINARKPAALEFLSQLLKETVTGPNPLALRADFASSLDAVGGWLKQDPTNKAALELAGDFQDSDRGNQDVDLEDREKRDQIEYIFAEWISLCQYLLTSEKNYSSFIMQLHQTKALADTASSCEFLRTCIEFCIAEYEQSTSTNPTAATNCYIPIDALAKLVVLLVKYQGEGDQKGQAVDKADYLNSTLALIVFVFNHHHEARGEHFSQKVFFRFFSSMLYEYHLIENQMMGYHERILETFAECFLTIQPAFFPMFSFHWMTLVCHRYFMPKLLRLEGEKGWPVFAKLLETLLVYVGAPLKERSIPPVVKLLYRGILRVLLVLHHDFPEFLAEWHFSICEVIPVHCTQLRNLILSAYPSSLSDLPDPFTAGLKVDRLPEVKKAPRISGDVFRPLIDSGIKQLLDSCLGSPEGLTNAAIQRIVEMLDTEPRKVTGLGFATITVDTAIINSLVLYLGMEAIREATEKGGSTFQTQSPHTALFSKLASDLKPQARYFFLSAIANHLRYPNSHTHYFSYLLLYLFGQVQGKTNESDVRQQITRVLLERLIVHRPHPWGLIITLLELLKNPTYDFWQLPFIKTAPEIERLFGALFQHINNSPLRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7L2</accession>
    <name>D5G7L2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00004607001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="3">Binds 2 manganese ions per subunit.</text>
    </comment>
    <dbReference type="EMBL" id="FN430031">
        <property type="protein sequence ID" value="CAZ80505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836314.1">
        <property type="nucleotide sequence ID" value="XM_002836268.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7L2"/>
    <dbReference type="STRING" id="656061.D5G7L2"/>
    <dbReference type="GeneID" id="9186984"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004607001"/>
    <dbReference type="eggNOG" id="ENOG502QQ0P">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030515_2_0_1"/>
    <dbReference type="InParanoid" id="D5G7L2"/>
    <dbReference type="OMA" id="ELHWHKA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033609">
        <property type="term" value="P:oxalate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20305">
        <property type="entry name" value="cupin_OxDC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017774">
        <property type="entry name" value="Bicupin_oxalate_deCO2ase/Oxase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006045">
        <property type="entry name" value="Cupin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051610">
        <property type="entry name" value="GPI/OXD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03404">
        <property type="entry name" value="bicupin_oxalic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35848">
        <property type="entry name" value="OXALATE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35848:SF9">
        <property type="entry name" value="SLL1358 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00190">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00835">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0464">Manganese</keyword>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin type-1" evidence="5">
        <location>
            <begin position="122"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="Cupin type-1" evidence="5">
        <location>
            <begin position="299"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="400"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="340"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="342"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="347"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="386"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR617774-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR617774-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ80505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="452" mass="49693" checksum="4E5D8A4FA0A31D50" modified="2010-06-15" version="1">MSAPAPPLSQAATKTYAVSGDLRGPTSLRGYDPELPFATESANRAPVQLAPGQDEDDPEGRILNFENVTEPQPLRGTRGGTIASNHDVELEKLHPDSFAPPTTDHGAVKQAEWPLGLSHVKLGLERAGWSRQQNINVLPSATEMAGVNMRLEAGGYRELHCKSRHWALMLNGSARIQAMNVDGQTFIDDVTKGDVWFFPSGVPHSIQGLENGTEFLLVFDDGEFSEDSTFLVSETFALNPKEVLTKNFQLPERAFDDIPEGELFIFPGTEAPEDIKKQNVTGPAGIIPNKESYSYHWSLQKPIKAPGGTVKILDSNTFPIAENFAAALVTIKPGAIREIHWHPDSDEWSYFLSGTARLTIYAAQGNSRTFDFRTGDVGYIPKSQTHYIENTGTEDVIVLEVLQAKRFTDFSVGQWLALTPPQVVQDTLHLSDESISQLSKDKPFIVDGPIPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7M7</accession>
    <name>D5G7M7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00004637001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAD-dependent oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430031">
        <property type="protein sequence ID" value="CAZ80520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836329.1">
        <property type="nucleotide sequence ID" value="XM_002836283.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7M7"/>
    <dbReference type="FunCoup" id="D5G7M7">
        <property type="interactions" value="379"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G7M7"/>
    <dbReference type="GeneID" id="9186999"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004637001"/>
    <dbReference type="eggNOG" id="KOG2495">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019845_6_1_1"/>
    <dbReference type="InParanoid" id="D5G7M7"/>
    <dbReference type="OMA" id="SFYWNIA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004174">
        <property type="term" value="F:electron-transferring-flavoprotein dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43735">
        <property type="entry name" value="APOPTOSIS-INDUCING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43735:SF3">
        <property type="entry name" value="FERROPTOSIS SUPPRESSOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="5">
        <location>
            <begin position="4"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ80520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="392" mass="42018" checksum="C85C7ACC08FEB5CE" modified="2010-06-15" version="1">MTSNIVIIGASYSGLPAAHYLLKHAPESWTITLINPSTNFYFNLASPRALVEQSILGKKNGDLFIPFLPKLEKHGSRFAFLHGKASAVNVGSRTVTVVLHGGRGETSVTYDHLIVASGANTSGGAGKEWGFKHGLDGNTEEIVANTRAAVRAAKKIVVSGAGATGVEFAGEIKDFYGAKKEVVMISSSEELLPQVPRADVGASARKILTKIGVKVKTGVKVIEERPVEKGIEGAVELVLSNGEVISADLHVATWGIYPNTSFLANDLLDGAGWVKTDEYMRVSGTRNVWAIGDVTHWGNRKVTTVDGQWAVATQNIVSSINGGGVEVFKKYVHSDALLMVVPMGRRFANAVAFINGWKVWGFVGWLFKGRTYMIENARPVAEGITTAGRAKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7S5</accession>
    <name>D5G7S5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">3-phytase</fullName>
            <ecNumber evidence="2">3.1.3.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00004705001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the histidine acid phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.8" evidence="2"/>
    <dbReference type="EMBL" id="FN430031">
        <property type="protein sequence ID" value="CAZ80568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836377.1">
        <property type="nucleotide sequence ID" value="XM_002836331.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7S5"/>
    <dbReference type="FunCoup" id="D5G7S5">
        <property type="interactions" value="411"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G7S5"/>
    <dbReference type="GeneID" id="9181387"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004705001"/>
    <dbReference type="eggNOG" id="KOG1382">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020880_2_2_1"/>
    <dbReference type="InParanoid" id="D5G7S5"/>
    <dbReference type="OMA" id="RGRSDWC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016158">
        <property type="term" value="F:inositol hexakisphosphate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07061">
        <property type="entry name" value="HP_HAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033379">
        <property type="entry name" value="Acid_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000560">
        <property type="entry name" value="His_Pase_clade-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20963:SF18">
        <property type="entry name" value="ACID PHOSPHATASE PHO11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20963">
        <property type="entry name" value="MULTIPLE INOSITOL POLYPHOSPHATE PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00328">
        <property type="entry name" value="His_Phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00616">
        <property type="entry name" value="HIS_ACID_PHOSPHAT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="521"/>
            <end position="553"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ80568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="553" mass="62350" checksum="B96E0A66D4FE9035" modified="2010-06-15" version="1">MGKKMNVNFKLVAIFPKRFRYLPIQMSLLLFTISAMICLLASVVFLLNPQPSYSLPNSLQQILPAYLQTNHHGWGLGLETACRGASNCPDQNREWNILYHLGGNGPWVEKVDGVVKGGIRVPDDCEIDMAHMMSRHGERFPTKNAGARMIVLLKKIRSSGVELKGSLAFLNDWQYFTDSPEKHFEQLTTTGPYSGTLEAFTTGVKLRTRYRHLWEKVLHNHTLFWASECDRVIDTARYFGAGFFGLENRHTELQIISEDESKAGDTLTPGDTCKTYVEDPELGHDYGYEMLYKYRATYLPAIAQRLEKENPGLQFSDSEIYSMQEMCGFEITVLGNSKWCNVFTRQEWSQFEYARDVIHYYRAGPGNKYAKAMGWLWLNTTANLIAEGPESAGPLYFSFVHDGDIVPMLAAFGIFEDTQDLPVDEMMEDRKWKTSQITPMGGRIIFERLSCTAKNKSAEKEIFVRFNVNDGIVPLEGCAGGPGESCPLNGFLEHVKRRGEIAGDFRTVCGLAHDAPDRPTFLRQPSPDSTMAAAAEGSEVKGDKPKKSRFWGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7X7</accession>
    <name>D5G7X7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00002623001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430033">
        <property type="protein sequence ID" value="CAZ80620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836429.1">
        <property type="nucleotide sequence ID" value="XM_002836383.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7X7"/>
    <dbReference type="GeneID" id="9181439"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002623001"/>
    <dbReference type="HOGENOM" id="CLU_479953_0_0_1"/>
    <dbReference type="InParanoid" id="D5G7X7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="224"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="281"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="234"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="251"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="281"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="325"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="568" mass="61054" checksum="331F2FE45B1B393A" modified="2010-06-15" version="1">MITIANSRHVRIWWAMNSPKEPMDLLFCVHRADGEAGTPPPGNIVFAPRNNRGTPSDESADSDDDGSSDGHQPESSARAAKRVTRPTTKTKNTSRNTGQTLRRGTVDVDEPELDSNVSADRGRNLATRVMPALNPGFAPSPGKQVPAPSTDLGTRVLRSGGQIGRGTDRKKASRLRTQTAVNSSPKCNLAAITDADEPNSPADAAEPPRNVARRVTAINTSIESELDRVHGEVNPSRRPTTRPITPNSPPLASNPSHNLASDASEQVQVRASAEHLLMLASGTQQPADTSATQVSTPSRSHSPPDPSTSDVLVSMSAAEAGRELSPAANSPASSSRYPTPEKLRLQLPGFSSATSAASSTAVSAETDVGSPLRFVSDLRAYVRFKEFTGTRPLRNLGISLLVFVRLDPAAGPVCPDPVWLIQTRHGCYRDKGRSLDIFFGCFRLVVFSFGGLVFSDFPGSLLQFFTHRSCSFAALVRSGYLCSSSFRLVALWFCADHSSFLAVFRSQQWFAVAPFIAFPIPDFHLIPHLLGLSGSLYLLRLASQALSPSLTRICFGPPPPLADLLELD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>D5G894</accession>
    <name>D5G894_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00002937001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430041">
        <property type="protein sequence ID" value="CAZ80737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836546.1">
        <property type="nucleotide sequence ID" value="XM_002836500.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G894"/>
    <dbReference type="GeneID" id="9188339"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002937001"/>
    <dbReference type="HOGENOM" id="CLU_1124258_0_0_1"/>
    <dbReference type="InParanoid" id="D5G894"/>
    <dbReference type="OMA" id="HRMCHQW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051678">
        <property type="entry name" value="AGP_Transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002575">
        <property type="entry name" value="Aminoglycoside_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21310:SF15">
        <property type="entry name" value="AMINOGLYCOSIDE PHOSPHOTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21310">
        <property type="entry name" value="AMINOGLYCOSIDE PHOSPHOTRANSFERASE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01636">
        <property type="entry name" value="APH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoglycoside phosphotransferase" evidence="1">
        <location>
            <begin position="58"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="258" mass="29510" checksum="F5C636E9F0B38E62" modified="2010-06-15" version="1">MDEQWLSHCLDENMTHSAPDRRVYVSENYIIKKRLSRDENGHSGYVPIYSAVRQRDENEILALKLVQEHTSVPVPKVIHSGNGFTVFERFSGVTVDDKETWEKVTPKQREAIKLQVQGYIQDLVKIPHPSSGIRSLVPEGILFHNQLPYPGPFQSTQDFLKAYDREDVFHEYLIKQIDPHSIPVFSHFDWSLANIVLSPNMDVVIGVIDWERACFFPEAGRCVHRMCHKLSGWETLFDGMEFPKDVSGGGDGREGTAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8L8</accession>
    <name>D5G8L8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00003003001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430047">
        <property type="protein sequence ID" value="CAZ80861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836670.1">
        <property type="nucleotide sequence ID" value="XM_002836624.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8L8"/>
    <dbReference type="STRING" id="656061.D5G8L8"/>
    <dbReference type="GeneID" id="9186229"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003003001"/>
    <dbReference type="eggNOG" id="KOG0581">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1023750_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8L8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="272" mass="27605" checksum="8232D974CCFCDF2B" modified="2010-06-15" version="1">MSSPLEEDPETPSSPDPFGDSASIFTTTSPPPSQPHRTIPSLRSISDSKTIPHSPNPLAPLNAARRPGPSQVPFASKSGTGGLPKDVQAKLQAFQASRRGAPPAAMGGGAPTLQTGVPQVVGPAMSPIGAPFSPAGGMTGTPGGRPAAPQSWTSAPAIAGRMGPPKMSLMERRRMQGFPGGLPPAPGAVPPGPLGGGSPITNGGTGATPTDGVQGSLFDKYSEYVDAKTGSLKFAGKAVLHSKGVDFSSGSSFRAIMEQCTKLNPRPRAERE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8Q5</accession>
    <name>D5G8Q5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00003095001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430050">
        <property type="protein sequence ID" value="CAZ80898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836707.1">
        <property type="nucleotide sequence ID" value="XM_002836661.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8Q5"/>
    <dbReference type="STRING" id="656061.D5G8Q5"/>
    <dbReference type="GeneID" id="9186266"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003095001"/>
    <dbReference type="eggNOG" id="KOG2940">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046586_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8Q5"/>
    <dbReference type="OMA" id="YEVVYGH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050602">
        <property type="entry name" value="Malonyl-ACP_OMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13090">
        <property type="entry name" value="ARGININE-HYDROXYLASE NDUFAF5, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13090:SF1">
        <property type="entry name" value="ARGININE-HYDROXYLASE NDUFAF5, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="4">
        <location>
            <begin position="78"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="307"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="319"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ80898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="329" mass="36326" checksum="2322C89E8D296325" modified="2010-06-15" version="1">MPPLPIPRPSGALRTLDTAIASPNPAYQVFNRRTKCLQRSRSTQNPAHSRATDYLKSEISLRLIDRVQDIQRPLPHILDLGAGACHIARQLPLGISSRLEKLTCVDMSRELLYRDAELEFNDNLPIERVVADEEVPPFAENTFDAVISSLSMHWINDLPYTLSSINRILKPDAPFIAAILGGDTLFELRTSLQLAELERRGGVSPRVSPLADVRDVGGLMQRAGLKLLTIDVDDVVVEFPDVFALMADLNAMGEGNAVSAREVGPIGRDVLMAAEGVYRELYGGEEGRLPATFRVIYMIGWKEGENQPKPLPRGSGEIPLKDILEGEKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8Z8</accession>
    <name>D5G8Z8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00004912001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430053">
        <property type="protein sequence ID" value="CAZ80991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836800.1">
        <property type="nucleotide sequence ID" value="XM_002836754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8Z8"/>
    <dbReference type="GeneID" id="9181274"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004912001"/>
    <dbReference type="HOGENOM" id="CLU_1031311_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8Z8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="244"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="258"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="270" mass="29855" checksum="FFFFC0390E437DBB" modified="2010-06-15" version="1">MSPPTKSVLLPKVFPLSLVNLGQLLPNPLNPSFSSCIPERPITEDDYSKLEESEYTATLTLEKSGKLKASFTKLFGAFFGVRTVNTIGLYARTAFHLELIQPDAIFRKVVQESEVQAWLNEMAQYHKPVYFVVGLQVIIDGVFKQRIDRSEEAGISMHVPLEAVGLPAHGSAQATGLVKGEGYVEGKVLGENVLGILVRKVNYKIRNSGDRPTLVEESVWKYPYERVKGDREEEALVLEVSIGDHVGDDDLGGMSDGLGEEDDEDDEDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>D5G997</accession>
    <name>D5G997_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00003209001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430056">
        <property type="protein sequence ID" value="CAZ81090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836899.1">
        <property type="nucleotide sequence ID" value="XM_002836853.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G997"/>
    <dbReference type="FunCoup" id="D5G997">
        <property type="interactions" value="180"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G997"/>
    <dbReference type="GeneID" id="9184376"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003209001"/>
    <dbReference type="eggNOG" id="ENOG502SHQK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007003_0_2_1"/>
    <dbReference type="InParanoid" id="D5G997"/>
    <dbReference type="OMA" id="TFWGCFT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051615">
        <property type="entry name" value="Transcr_Regulatory_Elem"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31313">
        <property type="entry name" value="TY1 ENHANCER ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31313:SF81">
        <property type="entry name" value="TY1 ENHANCER ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="9">
        <location>
            <begin position="70"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ81090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="712" mass="79069" checksum="9FA109A33306277E" modified="2010-06-15" version="1">MPENSGGAVEQGIMEGGEGEIQAYKSRKTSEAGGTDMVRRILPKQEEGNSPPLDSIIPARSAKRRCVSSACIPCRRRKSKCDGASPACSTCLAVYQTECTYDFDGDHRRKGALKRDIEQLKEKNDSLDVIVATIRSASESEVCEIVSHIREHGSLADIARSIKENTAMPDCEVHSGSETDLSDIVEKFRTGDSKHLCVLWSGVASVDGERVGRSDSRQCSGSWTRVTKDIEFISDLMSLYFTWCHPSYVLFSKSCFLSDMSRGRTKYCSSLMVNAILALACHYCDRKEARETGSLAVMADHFFQEAKRILVAEGETPCLTSVQALALMSLREAGCGRNSSGWMYSGRAFRMALDLGLNLPLDWQGFSPTETEVRKITFWGCFTLDKMWSVFLGRVPQLPSTIVGISKPNVFPPIESEPWQPYTDHGVPHGTPQSGRVQGVAIHFAEVSEILGDVLTLLYSPKERVTLQILNATYSRLKEWKAQLPEDMAPKATSLPSTLLLHLYYHCTIGLLFRPFFAKELNFFGENPREICRQAANDSAEVFGLFREGYSLRRTVITSSHIILAMAILMLVDLPSSPAVETTLVQCLKDLRELTSVWVWCTSALQGIAKLVERLNVELPEEAWGALSAPPSSPPMREQDINVIHASYDLMSGNSLPYSKVKIEVPHDLVEISEDSCDPSWDQLGNEDFQLVESMISFPSQFEDWGCIASDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9G1</accession>
    <name>D5G9G1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00003386001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430061">
        <property type="protein sequence ID" value="CAZ81154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836963.1">
        <property type="nucleotide sequence ID" value="XM_002836917.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5G9G1">
        <property type="interactions" value="1267"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G9G1"/>
    <dbReference type="GeneID" id="9182905"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003386001"/>
    <dbReference type="eggNOG" id="KOG0392">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000315_1_0_1"/>
    <dbReference type="InParanoid" id="D5G9G1"/>
    <dbReference type="OMA" id="WYSDIAC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000228">
        <property type="term" value="C:nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042790">
        <property type="term" value="P:nucleolar large rRNA transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045898">
        <property type="term" value="P:regulation of RNA polymerase II transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000508">
        <property type="entry name" value="Probable helicase mot1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044972">
        <property type="entry name" value="Mot1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022707">
        <property type="entry name" value="Mot1_central_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36498">
        <property type="entry name" value="TATA-BINDING PROTEIN-ASSOCIATED FACTOR 172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36498:SF1">
        <property type="entry name" value="TATA-BINDING PROTEIN-ASSOCIATED FACTOR 172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12054">
        <property type="entry name" value="DUF3535"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="1176"/>
            <end position="1354"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="1423"/>
            <end position="1580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="131"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="559"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="910"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="147"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ81154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1645" mass="181609" checksum="1C391F72A7BD6514" modified="2010-06-15" version="1">MKLEKEDEDEEGLGVLMGVALLGVESGLPPAGADDVLNFDTLDIAAVIRKGKRLLGSSGKEYDYTLADLDPAERLAAQKKNVTARLGLGGEYMDEEIVSERDFATVGHTVQTPRIDTSVGGTFRQPMASPALQSAMSPHSGVPGTPNDESASGLSRRQQNALKRKKKLDARNQANKTKIAVEFTGPKAPPSPIIQTSGEGFEWPFERLCELLLVDMFDPNWEIRHGAAMGIREVVRVHGAGAGREWGKSRKENNALNRKWLDDLACRLCCIFMLDRFGDYVADTVVAPIRESTSQTMGALLIHLPPPSVHAVYRVLYRLVMQEDLNLTLPIWEVCHGGMLGLKYLVAVRTDLLMKDNELLDGVVRAVMKGLGDQDDDVRAVSAATLIPIAAEFISLRPNAVDGLINVVWGCLSHLKDDLSASTGSVMDLQSFALLVPRLYPFLRHTITSVRAAVLRALLTFLNIQGDGTKGWVDGKILRLIFQNLLVERVESVLSLSLQVWMALTEELARDNPARFATEFGPHLDPLLTLLTTPVGVSRHPQPMDPSLFIRPSGQTMVLPGLMRGPSPSQPAQEPATKRRRKSEKKEEPTASPHNVDGHMIHGDVDLVGVETIIKTKIAGATALGKAISLWPREDAIRAFEPRLVPLLQSPHSTTQLVTAMIFEEYARITSEVTDLKAQFLAALTNFLNSERPSCYRDLVSYMHVVQAQCRSLLNAFRDIGKVPSSRIPTIAAVCQGESEAGPDAFSIEKAEKIVGDEFARLKRALGPTQKLMSSQALADAKGVAVAAVEEAKAAKAQRDVRVLAAAAGAYIASGQLPKKLNPVVHGVMESVKLEENVDLQRRSALSIAALVGICSRSGRIAVADKLTKNLNSFLCLDVSEVPIFHQLEDLEDNILSLRKEEDLKDHTNQADFEREAKEAKIKRRGAKEALEQLATTFGASLFEAVPKLRDCIETPLHIAFGGDLPMDIKDPNVTLGQEVVDGLSTIRALLPRFHPDLYPTIIGLFPLIIKALQSHFTVLRYAAAKCFATICSVITIPGMTALVQQVLKMFDNAHDLKCRQGSIECVYHLIHVMETDILPYVVFLIVPVLGRMSDPDNDVRLIATTAFATLVKLVPLEAGIPDPVGLPAELLEGRATERKFISQMLDIHKVEPFELPVTIKATLRSYQQEGVNWLAFLNKFKLHGILCDDMGLGKTLQTICIIASDHYLRAEEFKVSGNPDMRRIPSLIVCPPTLSGHWQQEIAQFAPFLTALCYVGSPTERARVRSQLGTTDIVITSYDICRNDIDNISQYNWNYCVLDEGHIIKNSKAKLTQANNVLELWSLFDFLMPGFLGTEKQEAGALAIEALHKQVLPFLLRRLKEEVLDDLPPKIIQNYYCELSELQKKLYDDFAKKQSKTIFEKVSKQLAKDKSNDSGAGSENPTETNAISQHRALIFCQLKEMLDIVENDVLKKLLPSVSYLRMDGGTEARKRQDIVTKFNGDPSIDVLLLTTHVGGLGLNLTGADTVIFVEHDWNPQKDMQAMDRAHRIGQKKVVNVYRLVTRGTLEEKIMSLQRFKLDVASTVVNQQNAGLATMETDQILDLFNVGDMETASKPAAEEDMVDDTTGKVITKGSKKLLDGIGELWDDSQYNDEYDLNNFIATLQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAC4</accession>
    <name>D5GAC4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="14">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00005235001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the SecY/SEC61-alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430075">
        <property type="protein sequence ID" value="CAZ81478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837287.1">
        <property type="nucleotide sequence ID" value="XM_002837241.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAC4"/>
    <dbReference type="FunCoup" id="D5GAC4">
        <property type="interactions" value="870"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GAC4"/>
    <dbReference type="GeneID" id="9185760"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005235001"/>
    <dbReference type="eggNOG" id="KOG1373">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031763_2_1_1"/>
    <dbReference type="InParanoid" id="D5GAC4"/>
    <dbReference type="OMA" id="PMMRQMF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005784">
        <property type="term" value="C:Sec61 translocon complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904680">
        <property type="term" value="F:peptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015450">
        <property type="term" value="F:protein-transporting ATPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005048">
        <property type="term" value="F:signal sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070843">
        <property type="term" value="P:misfolded protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031204">
        <property type="term" value="P:post-translational protein targeting to membrane, translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030970">
        <property type="term" value="P:retrograde protein transport, ER to cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006616">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane, translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3370.10:FF:000002">
        <property type="entry name" value="Transport Sec61 subunit alpha isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3370.10">
        <property type="entry name" value="SecY subunit domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002208">
        <property type="entry name" value="SecY/SEC61-alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030659">
        <property type="entry name" value="SecY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023201">
        <property type="entry name" value="SecY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019561">
        <property type="entry name" value="Translocon_Sec61/SecY_plug_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00967">
        <property type="entry name" value="3a0501s007"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006341">
        <property type="entry name" value="PRK08568.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10906">
        <property type="entry name" value="SECY/SEC61-ALPHA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10559">
        <property type="entry name" value="Plug_translocon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00344">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004557">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103491">
        <property type="entry name" value="Preprotein translocase SecY subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00755">
        <property type="entry name" value="SECY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00756">
        <property type="entry name" value="SECY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 10 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="98"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="143"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="168"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="197"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="267"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="308"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="384"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="439"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="463"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="Translocon Sec61/SecY plug" evidence="13">
        <location>
            <begin position="64"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF10559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ81478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="498" mass="54656" checksum="849E51C8505AB522" modified="2010-06-15" version="1">MLGQGKITRSQLAFSASLNNFAIMSGIRFLDLIKPFVPILPEVASPERKTPFRQRMMWTGLTLLIFLVMSQMPLYGIVSSDTSDPLYWLRMMLASNRGTLMELGISPIISSGMVFQLLAGTHLIDVNLDLKSDRELYQTAQKLFAIILSLGQATVYVLTGLYGQPSDLGAGVCLLLVLQLVVAGLIVILLDELLQKGYGLGSGISLFIATNICESIIWKAFSPTTVNTGRGPEFEGAVIALFHLLLTWDNKQRALREAFYRQNLPNVMNLLATLVIFASVIYLQGFRVEIPVKSSRYRGTRGTYPVRLFYTSNMPIMLQSALSSNVFLVSQMLYNRLPDNLLVRLLGVWEPKEGSAQLYAVSGISYYMSPPLNFTDALLDPLHTAFYILFMLTACAMFSKTWIEVSGSSPRDVAKQLKEQGLVMAGHREQSMYKELKRVIPVAAAFGGACIGALSVASDLLGALGSGTGILLAVTIIYGYFEIAAKEGDMSGLGQFIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAI4</accession>
    <name>D5GAI4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00003603001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I 49 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430078">
        <property type="protein sequence ID" value="CAZ81527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837336.1">
        <property type="nucleotide sequence ID" value="XM_002837290.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAI4"/>
    <dbReference type="STRING" id="656061.D5GAI4"/>
    <dbReference type="GeneID" id="9187467"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003603001"/>
    <dbReference type="eggNOG" id="KOG2870">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015134_6_0_1"/>
    <dbReference type="InParanoid" id="D5GAI4"/>
    <dbReference type="OMA" id="GKNSEWN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006120">
        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.645.10">
        <property type="entry name" value="Cytochrome-c3 Hydrogenase, chain B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001135">
        <property type="entry name" value="NADH_Q_OxRdtase_suD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022885">
        <property type="entry name" value="NDH1_su_D/H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004739">
        <property type="entry name" value="PRK06075.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11993:SF10">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11993">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 49 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00346">
        <property type="entry name" value="Complex1_49kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="HydB/Nqo4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH-quinone oxidoreductase subunit D" evidence="2">
        <location>
            <begin position="9"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ81527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="279" mass="31210" checksum="D8A325742AFDC621" modified="2010-06-15" version="1">MSVLSHAMDVGALTPFLWGFEEREKLMEFYERVSGARLHAAYVRPGGVHQDLPVGLLDDIYQWATQFGDRIDETEELLTDNRIWIARTKGVGVVSAAEAINYSFTGVMLRGSGVAWDIRKSAPYDAYGQVDFDVPVGQAGDCYDRYLCRMEEFRQSLRIIHQCLNKMPPGPIRVEDYKISPPPRVAMKENMEALIHHFLLYTKGYAVPPGETYSAIEAPKGEMGVFVVSDGSERPYRCKIRAPGFAHLGSFDQISRGHLLADAVAIIGTMDLVFGEVDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAV0</accession>
    <name>D5GAV0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">serine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Seryl-tRNA synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Seryl-tRNA(Ser) synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00005319001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.11" evidence="2"/>
    <dbReference type="EMBL" id="FN430086">
        <property type="protein sequence ID" value="CAZ81643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837452.1">
        <property type="nucleotide sequence ID" value="XM_002837406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAV0"/>
    <dbReference type="FunCoup" id="D5GAV0">
        <property type="interactions" value="758"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GAV0"/>
    <dbReference type="GeneID" id="9187811"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005319001"/>
    <dbReference type="eggNOG" id="KOG2509">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023797_0_2_1"/>
    <dbReference type="InParanoid" id="D5GAV0"/>
    <dbReference type="OMA" id="GYTPCFR"/>
    <dbReference type="UniPathway" id="UPA00906">
        <property type="reaction ID" value="UER00895"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004828">
        <property type="term" value="F:serine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006434">
        <property type="term" value="P:seryl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00770">
        <property type="entry name" value="SerRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000026">
        <property type="entry name" value="Seryl-tRNA synthetase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.40">
        <property type="entry name" value="Serine-tRNA synthetase, tRNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002317">
        <property type="entry name" value="Ser-tRNA-ligase_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015866">
        <property type="entry name" value="Ser-tRNA-synth_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042103">
        <property type="entry name" value="SerRS_1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033729">
        <property type="entry name" value="SerRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00414">
        <property type="entry name" value="serS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11778">
        <property type="entry name" value="SERYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02403">
        <property type="entry name" value="Seryl_tRNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001529">
        <property type="entry name" value="Ser-tRNA-synth_IIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00981">
        <property type="entry name" value="TRNASYNTHSER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="tRNA-binding arm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="13">
        <location>
            <begin position="189"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="66"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="271"/>
            <end position="273"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="271"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="287"/>
            <end position="290"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="384"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for serine binding" evidence="10">
        <location>
            <position position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001529-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001529-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ81643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="444" mass="50700" checksum="715EA35F9DF380DF" modified="2010-06-15" version="1">MLDVLSFIEARGGNPEVIREAQRRRYAPVEAVDEVIALYEEGRVARFNVDNKGREINTTQKEIGAKKKAKEDASLLLQQKVKFEAEKKDLEVIAKEKEKQLYAKLGTIGNIVHNSVPVSNNEDSNELIRSWTPDGVNVEKHDCLSHHDVLARLDGYEAERGVKVVGHRGYFLKQWGVFLNQALINYGLEFLASKGYTPLQTPQFMLKDMMAKTAQLEQFDDELYKVVDGEEKNDKYLIATSEQPISALHADEWLQQKELPIRYAGFSSCYRREAGAHGKDAWGIFRVHQFEKIEQFVITDPEKSWEAFDEMINYSEEFYKSLNIPYRVVTIVSGALNNAAAKKYDLEAWFPFQGEYKELSRNLEIRYGTKAQTDTVKKYVHCLNSTLCATERSLCCILENYQTPEGINVPEALRKYIPGAPVFIPFSKELATNSTSMRRGKPKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAX3</accession>
    <name>D5GAX3_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">V-type proton ATPase subunit a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00005345001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Essential component of the vacuolar proton pump (V-ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the V-ATPase 116 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430086">
        <property type="protein sequence ID" value="CAZ81666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837475.1">
        <property type="nucleotide sequence ID" value="XM_002837429.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAX3"/>
    <dbReference type="FunCoup" id="D5GAX3">
        <property type="interactions" value="370"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GAX3"/>
    <dbReference type="GeneID" id="9187834"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005345001"/>
    <dbReference type="eggNOG" id="KOG2189">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005230_0_2_1"/>
    <dbReference type="InParanoid" id="D5GAX3"/>
    <dbReference type="OMA" id="FYLWFFL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000220">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051117">
        <property type="term" value="F:ATPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002490">
        <property type="entry name" value="V-ATPase_116kDa_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026028">
        <property type="entry name" value="V-type_ATPase_116kDa_su_euka"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629:SF63">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629">
        <property type="entry name" value="VACUOLAR PROTON ATPASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01496">
        <property type="entry name" value="V_ATPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001293">
        <property type="entry name" value="ATP6V0A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="425"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="462"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="549"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="579"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="641"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="724"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="753"/>
            <end position="776"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ81666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="820" mass="93669" checksum="E5952AA56C5DD383" modified="2010-06-15" version="1">MSGQDTLFRSAKMSLVQLYVATEISREAISELGEVGMLQFRDLNAETSAFQRTFTKDIRRLDNVERQLRFFGAQMEKNSVPVRPVPDNASLTAAPSASEIDYLATRADELEVRVSQLNASHETLQKRWVELVEFRWVLREAGGFFDRAHGQAEEIRRGSTDEPTAPLLEDVEQSTPAGSAGHVDTMFQTMNIGFVAGVIRRDRIAAFERILWRTLRGNLYMNQSEIDEPIVDPSTNEPVDKNVFVIFAHGKEILAKIRKISESLGAGLYSVDEDNTLRRDQLHEVNSRINDLDSVLHNTRNTLTSELRMIAQNLASWMVVIKKEKAIYQTLNLFNYDSARKCLIAEGWLPTNQLPLIQKRLRDVTDRAGLQVPTIVNELRTTKTPPTYHKTNKFTVGFQTIVDAYGVAKYREVNPGLAAIVTFPFLFAVMFGDIGHGLILLLTAAVMIWYEKKLEKQKVFELFDMVFFGRYIMLLMGVFSVYTGLIYNDVFSKPLTLFKSQWVYPEHFKIGEGIEAQKVPGYTYPFGLDWQWHGAENSLLFTNSLKMKLSIIMGWFHMTYSLCLSQYNHRYFKSRIDTWGNFIPGMIFFQSIFGYLVLTIVYKWSVDWVALDKNPPSILNLLIYMFLSPGTVTEPLYPGQATIQVLLLLVALACVPWMLLLKPLYLRWEHNKHHAAGYQGLGQVSREMKEEEEFEFSEVMIHQVIHTIEFCLNCISHTASYLRLWALSLAHAQLSIVLWDMTMAVGFGFEGTIGVIMMVILFSFWLNCTLAVLVIMEGTSAMLHSLRLQWVEAMSKFFIGDGIAFEPFSFKLLLEEAADQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5GB16</accession>
    <name>D5GB16_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glutathione peroxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00005402001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glutathione peroxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430086">
        <property type="protein sequence ID" value="CAZ81709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837518.1">
        <property type="nucleotide sequence ID" value="XM_002837472.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GB16"/>
    <dbReference type="FunCoup" id="D5GB16">
        <property type="interactions" value="544"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GB16"/>
    <dbReference type="PeroxiBase" id="11113">
        <property type="entry name" value="TmelGPx01"/>
    </dbReference>
    <dbReference type="GeneID" id="9187950"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005402001"/>
    <dbReference type="eggNOG" id="KOG1651">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029507_3_1_1"/>
    <dbReference type="InParanoid" id="D5GB16"/>
    <dbReference type="OMA" id="QCGLTKQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061692">
        <property type="term" value="P:cellular detoxification of hydrogen peroxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00340">
        <property type="entry name" value="GSH_Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000889">
        <property type="entry name" value="Glutathione_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11592">
        <property type="entry name" value="GLUTATHIONE PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11592:SF78">
        <property type="entry name" value="GLUTATHIONE PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00255">
        <property type="entry name" value="GSHPx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000303">
        <property type="entry name" value="Glutathion_perox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01011">
        <property type="entry name" value="GLUTPROXDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51355">
        <property type="entry name" value="GLUTATHIONE_PEROXID_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 4" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ81709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="158" mass="17882" checksum="A31054273269F996" modified="2010-06-15" version="1">MSSATTFYDLEPLDSRNQPYTFNQLKGKVVLIVNVASKYQYEGLEKLYEKYKDQGLEVLGARDRRGDPRCKRVNYGVKFPVLAKIDVNGGDASPVYKWLKDEKAGLLGIKLIKWNYEKFLVSREGKVVDRYASTTKPDSLASAIEKELNKGTPPKAEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>D5GB56</accession>
    <name>D5GB56_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00005460001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430086">
        <property type="protein sequence ID" value="CAZ81749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837558.1">
        <property type="nucleotide sequence ID" value="XM_002837512.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GB56"/>
    <dbReference type="STRING" id="656061.D5GB56"/>
    <dbReference type="GeneID" id="9181460"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005460001"/>
    <dbReference type="eggNOG" id="KOG3028">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055680_0_0_1"/>
    <dbReference type="InParanoid" id="D5GB56"/>
    <dbReference type="OMA" id="RNAWLYT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001401">
        <property type="term" value="C:SAM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03193">
        <property type="entry name" value="GST_C_Metaxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033468">
        <property type="entry name" value="Metaxin_GST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050931">
        <property type="entry name" value="Mito_Protein_Transport_Metaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012336">
        <property type="entry name" value="Thioredoxin-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12289">
        <property type="entry name" value="METAXIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12289:SF44">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN (SAM35), PUTATIVE (AFU_ORTHOLOGUE AFUA_1G13180)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17171">
        <property type="entry name" value="GST_C_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17172">
        <property type="entry name" value="GST_N_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin-like fold" evidence="2">
        <location>
            <begin position="58"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Metaxin glutathione S-transferase" evidence="1">
        <location>
            <begin position="213"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ81749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="297" mass="32969" checksum="3265CB1CF2828CD4" modified="2010-06-15" version="1">MASLPIPQFLKTLFDSFPLLTYPANSLPSRCPTFDAVPSLYVFTTSEDAVSGNPSFNPGCLKWQAYMKFNQIEFKTVPSNNHASPTGALPFVISQRRSMSESMELAVVPSNKLAAWIEKHGYSFEGMVGMDSARAEAFLALVNSQLRDAWLYSLYLEPNNSQDLASVLYCGSSVSAVSSFLLRQTKAAARQQLLQAHPGGVLDPRKIYADAGAALQAISTILGNHMWFFGGSNPGLFDASVFAYTHLILTLRWHSEENALLRAVMGHDNLLGHEERIRCEFFREAKTPNLNHWVWEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>D5GB85</accession>
    <name>D5GB85_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="11">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00003786001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430087">
        <property type="protein sequence ID" value="CAZ81778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837587.1">
        <property type="nucleotide sequence ID" value="XM_002837541.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GB85"/>
    <dbReference type="STRING" id="656061.D5GB85"/>
    <dbReference type="GeneID" id="9181488"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003786001"/>
    <dbReference type="eggNOG" id="KOG0207">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001771_0_2_1"/>
    <dbReference type="InParanoid" id="D5GB85"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043682">
        <property type="term" value="F:P-type divalent copper transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055070">
        <property type="term" value="P:copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000001">
        <property type="entry name" value="ATPase copper transporting beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017969">
        <property type="entry name" value="Heavy-metal-associated_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43520">
        <property type="entry name" value="ATP7, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43520:SF32">
        <property type="entry name" value="COPPER RESISTANCE P-TYPE ATPASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01047">
        <property type="entry name" value="HMA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="221"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="270"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="319"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="360"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="536"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="897"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="10">
        <location>
            <begin position="30"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ81778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="935" mass="101528" checksum="2EC05F3ADB50021D" modified="2010-06-15" version="1">MESTVSVNIARGLTPGTLAECVDSSGVALYEAVFSIGGMTCASCSNRITEAVESLKWVQSVRVDLLSNSATVLFDGMGCGGPEVGAEAILQQVEDTGFDCTLEELDVAGSRKRKSERGTVGAERIVSLKVDGMSCTGCADKVVEVIGFSFPDNVLEIESPPITLTSPVIHVRYKPQPPEFTVRHIASVISSISPDFVVSVYRPPTIEDRSRQLQQMEQWRLLLRLLFCLAIAIPTFLIGIVWMTFVPESDPVRQSLSQPMWTGRSTKMEWALFFLATPVMFFIADVFHKRAIRDIRALWRKGSPTPVLRRFYRFGSMNLLISLGVSISYFASLVLLVINAASKPAVQARHGLQKRRTSTYTYFDSTVFLTMFLLIGRFLEAYSKRKTAEAVNILGKLRPAEALLVDNRLWRIKAVPTDQLEVGDIVSVVRGSSPPADGTIISGVSQFDERPLTGEARLVPKIEGDVLLSGAVNQGQAVNMRVESIGSDTMLSRIVNVLREGQTRRAPIERLADLITGYFVPVYLDIRYGGWSAWSLSFAIAVFVIACPCGIGLAAPTALYVGSGLAAKFGILAKGGGEAFQEAAWLDCIVFSKTGTLTQGNDPKVTDELMLVGAGEDRRMAYAVAQQLEESSSHPLSRAIIAHCERKDKVPITMWDIEDIPGRGLCGVFKIANNKTDVFEAVLGSERFMEENGVLDMAYHDQVLSSWKSCGKSVILLAIRKQAVSGKVYDSRFRLVALYAAADIIRSEAPEVVRKLQESGIGVWMISGDNTTTAISVASMVGIPREHVISGVHPAEKVLVPFLRYLPCLPSIPFFQSSSPTRSSPTAAKSRATIALVGDGINDAPALSISSVGISMNSATDVNITSAKFILVSSNLNSLITLTQLASFVFRRVKVNFMWACVYNVLAVPVAAGALYAGAGIRVGPVWAAGIMAFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBE7</accession>
    <name>D5GBE7_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Elongation factor G, mitochondrial</fullName>
            <shortName evidence="10">EF-Gmt</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Elongation factor G 1, mitochondrial</fullName>
            <shortName evidence="10">mEF-G 1</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Elongation factor G1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">MEF1</name>
        <name evidence="12" type="ORF">GSTUM_00000435001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Mitochondrial GTPase that catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Protein biosynthesis; polypeptide chain elongation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430093">
        <property type="protein sequence ID" value="CAZ81840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837649.1">
        <property type="nucleotide sequence ID" value="XM_002837603.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBE7"/>
    <dbReference type="FunCoup" id="D5GBE7">
        <property type="interactions" value="640"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GBE7"/>
    <dbReference type="GeneID" id="9184420"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000435001"/>
    <dbReference type="eggNOG" id="KOG0465">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002794_4_0_1"/>
    <dbReference type="InParanoid" id="D5GBE7"/>
    <dbReference type="OMA" id="GQFAKVQ"/>
    <dbReference type="UniPathway" id="UPA00345"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070125">
        <property type="term" value="P:mitochondrial translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01886">
        <property type="entry name" value="EF-G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16262">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01434">
        <property type="entry name" value="EFG_mtEFG1_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04091">
        <property type="entry name" value="mtEFG1_II_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000003">
        <property type="entry name" value="Elongation factor G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.870:FF:000001">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000022">
        <property type="entry name" value="Elongation factor G, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.240:FF:000015">
        <property type="entry name" value="Elongation factor G, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000558">
        <property type="entry name" value="Elongation factor G, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00054_B">
        <property type="entry name" value="EF_G_EF_2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009022">
        <property type="entry name" value="EFG_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047872">
        <property type="entry name" value="EFG_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004540">
        <property type="entry name" value="Transl_elong_EFG/EF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005517">
        <property type="entry name" value="Transl_elong_EFG/EF2_IV"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43636">
        <property type="entry name" value="ELONGATION FACTOR G, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43636:SF2">
        <property type="entry name" value="ELONGATION FACTOR G, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03764">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00889">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="8 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Tr-type G" evidence="11">
        <location>
            <begin position="103"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="112"/>
            <end position="119"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="187"/>
            <end position="191"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="241"/>
            <end position="244"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ81840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="773" mass="85604" checksum="08BC2C0BB9C95125" modified="2010-06-15" version="1">MRAAGVKYKFRTLRLSPTSTTTGTRALRIPTTTASKPPKCLVPYSPSSCPFLRVGHPLALSKASSIRGLAAVAVAPAVPEEPDIIPQEEILAKMDPKEMARLARVRNIGIAAHIDSGKTTCTERVLFYTGRIKSIHEVRGKDAVGAKMDSMDLEREKGITIQSAATFCDWVKKENGVDEKYHINLIDTPGHIDFTIEVERALRVLDGAVMILCAVSGVQSQTITVDRQMKRYNVPRISFVNKMDRMGANPWKAIEQINSKLKIPAAAIQVPIGGEDSFQGVVDLVGMKAIFFEGHKGIDVIEKELSEIPAEVKKLAEEKREVLIAALADVDDTIAELWLEEKTPTLEQIKAAIRQATLARKFTPVLIGSALADKGVQPMLDAVCDYLPNPSDGNFGQLTYIRVYQGTLKKGMHFYNAKSGKKIKVPKIVRMHSDEMEEVPSIGAGEICAVFGVDCASGDTFTDGGLPYSMTSMFVPDPVISLSIKPKNKETAAFSKAMNRFQREDPTFRCKVDLESQETIISGMGELHLEIYVERLRREYGLDCVTGKPEVAYRETIDSRQEFDYVFKRQTGGAGDYARVMGYMEPTRDPEGKNHFEEQIVGGHISEKFLYACEKGFLKSTEKGPMIGHKVLGTRMIINDGATHMVDSSEMSFKMATEYAFRDTFKLANPIILEPIMKVAVSAPNEFQGNIVGLMNKRNAVIEDSEIGADEFTLTAMCSLNAMFGFSSQLRAATQGKGEFSMEFKDYLPTPPQLQKELIANFEKERARRNATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBK3</accession>
    <name>D5GBK3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00005674001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ82009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837818.1">
        <property type="nucleotide sequence ID" value="XM_002837772.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBK3"/>
    <dbReference type="GeneID" id="9185444"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005674001"/>
    <dbReference type="eggNOG" id="KOG4650">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043418_0_1_1"/>
    <dbReference type="InParanoid" id="D5GBK3"/>
    <dbReference type="OMA" id="WRKGGYQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010721">
        <property type="entry name" value="UstE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32251">
        <property type="entry name" value="3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32251:SF23">
        <property type="entry name" value="3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE (DUF1295)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06966">
        <property type="entry name" value="DUF1295"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="193"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="343" mass="39034" checksum="F4C8FE251362A4BB" modified="2010-06-15" version="1">MAISLPLPEVSSLSDTTDFSKTVTPFLHQLSISHIQPLLTGQVPVKEWYLSTNPLITAAHFALAMTLIVLVTSEINRNYSQVDRLWSLLPTIYIGHYTLFAHMNGLDTRRLNTLSAFSLLWSIRLTYNYWRRGGYNIGSEDYRWEIVQKSIPRWGFILLNIVFISFIQNVLLAMITAPAYIHLLASTLPTAPAWSFTDVLLSRSLLLILFVEAFADQQQWVYQSAKTAYLATGIVPRGYTKADLDRGFVITGLWSFCRHPNYTAEQIIWALVYQWSCLVCDELWNWTGLGAVAYMALFQASVRLTEEITAGKYREYKEYQKMVNKFVPGLGALTGASGFEKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBL4</accession>
    <name>D5GBL4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00005689001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ82020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837829.1">
        <property type="nucleotide sequence ID" value="XM_002837783.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5GBL4">
        <property type="interactions" value="42"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GBL4"/>
    <dbReference type="GeneID" id="9185455"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005689001"/>
    <dbReference type="eggNOG" id="ENOG502QU87">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001720_0_0_1"/>
    <dbReference type="InParanoid" id="D5GBL4"/>
    <dbReference type="OMA" id="TKVEWLW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd07609">
        <property type="entry name" value="BAR_SIP3_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13280">
        <property type="entry name" value="PH_SIP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000349">
        <property type="entry name" value="Transcription factor SipA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039463">
        <property type="entry name" value="Sip3/Lam1_BAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042067">
        <property type="entry name" value="Sip3_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031968">
        <property type="entry name" value="VASt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14248">
        <property type="entry name" value="CYCLIN Y, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16746">
        <property type="entry name" value="BAR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16016">
        <property type="entry name" value="VASt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51778">
        <property type="entry name" value="VAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="PH" evidence="6">
        <location>
            <begin position="295"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="VASt" evidence="7">
        <location>
            <begin position="887"/>
            <end position="1058"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="521"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="564"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="610"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="534"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="582"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="636"/>
            <end position="649"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ82020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1363" mass="151371" checksum="F1CDE5BBFD6C3803" modified="2010-06-15" version="1">MLLRPVSLVPVTLREAALDSPTFRGTVVHYGEQVELVEKWLEGYLKATLKLCSELDVGLEEAVNSYLGKGVPNGLSESIIDQDYTLLAIRRFAEGSRGFWGAVINGAKKVEQTVVEPLLGFQRSELRVFKDSRRSLETTQAKYDSLLARYAGLSKSKEPSSLREDAFQLYEARKLYIRACLDFCVAAPIFRASLDRTLTKVLSDQSRKQIRLRREGTAAAEKHAVEMDRIRAWSEGMEQSDKVFRKELLMARKELEEKVKRELHPARELEEYASSTVPYLSSKGPGIAPEGENISSEKQGWLFMRTLTGKPTRSLWIRRWFFVREGIFGWLMQGYKGGAMEESEKIGVLLCNIKPAFQEERRFCFEVKTKDTTILLQSETQAELTSWLAVFEQAKRTAIESASSTTSTQAFSIIPPSAPVLASEPAYVAKGHDGNNASSALASQPTGKDAISLNPLSMSRHLLVHRERDDEASGIDRSLTMPLGALGGASRGTSFDISRIDNSTLVSSGAREKIAQKLDIRRGTGYGERNQSLAVPPASPGNSNSSSGIGAGVSALISASHNALPFHPPASPPTDGKFADGPTSLAPATLASTPTPATLSKAAMVTLGQEPINDDFGSKGHRKTQSLDETAGPHLLRRDDNPPGGKDEYPSGYPPELRAQDNHFRVLFPGRRPGEAVLLVFRATWSPNDTQELPGRCYVTVENVYFYSHYLGLVFTSVIPLTSVTEVKAAPGKDCDYLFLHLDPEDGNVGVDQEGHEPHITVKIFLEPVRLLQRRLLFLVKNANASSEDEPPKLPARKVLERLIALEREVEERTGTDTESWEDVGFVDVHDELERRKRTGELVRVRTDGDILTPNPTATQGSIDGRVREVTRFKLPSVPVVYEPGGMDKKAFEREFEVSPKAMFHVMFGDKSAVFQMLYNERRARYIQQGAWTQLEGGSMRREFKYQIEYLDILRRLRISNVVDHQTIEKQDDHLCYVITDRKTPWHLPHREHFMLVSKVVITHLAKSKCKLSIWTAVEWAKEPTFSKGIVHRQALDDLDLDALDLGDVVADQARKLGSPSRIENAVHIFGGIGQETPGSHLTAGGPDGAPLAPDLDPRAPRLPIAHRSLSQMMLETGLSFLESAVSSLMMWTFTALQKAWGIASAQRIILLFLAVSLLSNTMLSSRSTVSYWNERRAANFMNAVGVSPNGIMSRAVYLKDMDEMVFNGTDLAHDGSSQCYAKFRELASFTDMDADAALASKGFFSASTRATARRLRLSRQHLGSYRHDLLVAMRVVNSLEREMVHAEWDNWLYEETSRCKQALFILNSRKQPAGGTGLNSTSEGGGRGEHGEAAKWLSEYCDSCNSERSESDTGRRLGLAFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBP2</accession>
    <name>D5GBP2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00005502001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ81892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837701.1">
        <property type="nucleotide sequence ID" value="XM_002837655.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBP2"/>
    <dbReference type="GeneID" id="9182929"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005502001"/>
    <dbReference type="HOGENOM" id="CLU_2591518_0_0_1"/>
    <dbReference type="InParanoid" id="D5GBP2"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="80" mass="9457" checksum="A836E7B4DD46E7A6" modified="2010-06-15" version="1">MIPHPKNIWKGLYCATTNKITNYSSSTRYSLFTISRQLDFFFYGTKPLKLFGPTEQVLPMAPRKRSSRSRRKREQKARDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5GC14</accession>
    <name>D5GC14_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00005761001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430098">
        <property type="protein sequence ID" value="CAZ82057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837866.1">
        <property type="nucleotide sequence ID" value="XM_002837820.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GC14"/>
    <dbReference type="STRING" id="656061.D5GC14"/>
    <dbReference type="GeneID" id="9181326"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005761001"/>
    <dbReference type="eggNOG" id="KOG0428">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041481_0_3_1"/>
    <dbReference type="InParanoid" id="D5GC14"/>
    <dbReference type="OMA" id="ISGHHED"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23799">
        <property type="entry name" value="UBCc_UBE2J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000093">
        <property type="entry name" value="Ubiquitin conjugating enzyme (UbcF), putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="3">
        <location>
            <begin position="10"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="201"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="209"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ82057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="292" mass="31974" checksum="F179882466FC1568" modified="2010-06-15" version="1">MSQTFNTKSPTIKRILREAEELSKNPAEDYHAAPLDENLFEWHFTLRGPPGTPYAEGIYHGRITLPPHYPLRPPSFRFMTPSGRFEMNREICLSISGHHEDTWQPAWGVRTAVVALRGFMETEVKGQVGGIECDDETRRGIAKETGEWRCGACGGRSNGEILEGCRILAEEMGKGKQVGEGGAEGSVEGIKIGYRDEASKAQGGGATIAESSSVTAGELDTSTAHQPTTLPNPHPHQHSRPSLLPMLQPQLQLQLPLRAPAQETQTPWLDKLILALIAVLIAHVIKKIASHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCF4</accession>
    <name>D5GCF4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Spindle pole body component</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00005865001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
            <location evidence="5">Cytoskeleton</location>
            <location evidence="5">Microtubule organizing center</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the TUBGCP family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430109">
        <property type="protein sequence ID" value="CAZ82197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838006.1">
        <property type="nucleotide sequence ID" value="XM_002837960.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCF4"/>
    <dbReference type="STRING" id="656061.D5GCF4"/>
    <dbReference type="GeneID" id="9186379"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005865001"/>
    <dbReference type="eggNOG" id="KOG2000">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006331_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCF4"/>
    <dbReference type="OMA" id="DYCFHVG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000930">
        <property type="term" value="C:gamma-tubulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043015">
        <property type="term" value="F:gamma-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051011">
        <property type="term" value="F:microtubule minus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1900:FF:000013">
        <property type="entry name" value="Spindle pole body component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1900">
        <property type="entry name" value="Gamma-tubulin complex, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007259">
        <property type="entry name" value="GCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040457">
        <property type="entry name" value="GCP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042241">
        <property type="entry name" value="GCP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041470">
        <property type="entry name" value="GCP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302">
        <property type="entry name" value="GAMMA TUBULIN COMPLEX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302:SF70">
        <property type="entry name" value="GAMMA-TUBULIN COMPLEX COMPONENT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04130">
        <property type="entry name" value="GCP_C_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17681">
        <property type="entry name" value="GCP_N_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3 5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma tubulin complex component protein N-terminal" evidence="8">
        <location>
            <begin position="206"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="Gamma tubulin complex component C-terminal" evidence="7">
        <location>
            <begin position="631"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="998"/>
            <end position="1020"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ82197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1020" mass="113366" checksum="1DDE21ECE18FD54D" modified="2010-06-15" version="1">MSTSPSSRQARTHSIMTHFSSVFPNPRPFMKYGDKGEEGKEGYPFIIPDLWKPSCFAPDDSAQPTLFPIAPLQETVLNITKPLDPYKDESEVNLFTFDHDFAEYFADPDPGSPTEVDSIASTAGEAVEQESSDTSEDIWLRPGSSDLPVNPRFQTWESFLSAGANNPANPYLSEAGSQVFDAVLTEYVDGSEEITEVVRTDVLCTCLLHLGLGRNSVLFTYDRKKAEFLSIKPRLRISGCTAGTVDSIIKSFADCGKKIKQLQKFVDDVGNDSRVATPSTIALADCITAIVESIRQRLSVDPATISSVLHLHSLMREPSLIIDTFHQIISQTAGISDDSRLLTTLFEVVQQEQHRSGWLKPLLMEALARVTRPWLESIEEWIGLGRPAGGEGIGFKGSVERGFFVRVEEETYVNERGAERTNRNFIFDKAKVPSFMSAESGESLFESGKSLRFLQKFHPSHPLSRPGAVEGARAPRLEWKFSWEDLNNLQSQAMSYERELRLAIDKYSVQGSTATAPMAGNYQSVPGGIDAFETFGKSKEEIGAMIGKSVSAMNSPPLSIEHLHKKDLLRTLVLKISRLELNPDPGHDHLTAFAPPISITPVLSFSHIFAVQAKIINTACLNMFFKGHKVREHLSLLRKFELFGDGVYSSRLSHALFGDELDGTEKRGGVHRTGGTMGLKLGSRDSWPPASSELRLALMGVLDESFDNTQTKGGIHHAGKDGDLPGGLSFGVRDMTMGELERCMDPNGLEALDFLKLQYKPPSPLDAIISTTALYQYDRLFKLLLRVLRMLFVVNKLFRDATSRGSRWHTVDPVAQKFRIEARHFVDAVCSYMFETGVGSTWRKFEDKLQDIERKLDSHDSDLGAEGIESLRVYHESILDRIMFATLSRKRQAPLMKLMEDIFTSILTFAKYSHAKAIGAQTNDEEIPVLYAGFKRRIGIFINVCRGLSEKRGYGDGVKDSEGRRAVDAIFGSGSKEEGNLLSMLLLKLEMTEYYTDTGKGEREKIKKGGRGRKGPSDPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCK2</accession>
    <name>D5GCK2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00000673001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430111">
        <property type="protein sequence ID" value="CAZ82245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838054.1">
        <property type="nucleotide sequence ID" value="XM_002838008.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCK2"/>
    <dbReference type="STRING" id="656061.D5GCK2"/>
    <dbReference type="GeneID" id="9186427"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000673001"/>
    <dbReference type="eggNOG" id="ENOG502S9CJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_537696_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCK2"/>
    <dbReference type="OMA" id="MRIYPPR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="507" mass="57223" checksum="46CA350BE21ABACF" modified="2010-06-15" version="1">MCCVFTLRLPFPILAPLHHKNHNSPSPITNSAYLLSRPAVRFLPIALATMFCNWAAVAATSIRGRDRSPSSGPEIKPPVPFTGPKIFPRYSPPQTSGSSFTAPPQERSFYTFFGRIKKATEVTPEHLNALGVLVEYDVPVEKLLPSGGASFIPVDDDKIFSERRRELLIPNDDAVEALARRRKDVKLGHMYRFYQGVDMVKTYYPTEPPSDPLTPMSSGDEAGKNGKRKAEEVVEGGVEKKGKPEGPSATAPTPLSDLPEINEEEDGRKFSMPEKFREELVKNFIEPICWGYGVRIYQSPPRAPPRVALQRSRFLVHMTNYIYLTPALPAEARSGLVEGPVMGVQVRHEFEFLKKQSDDSMDVDGEEEVGDVVGITREVAAALSIAQDRRKGPREGRPEGKTEKKIRFRAVGKDDKWRDDIFLLTSLHHHISITHISVTQPYLRFLETGQMPNLNHEYFQTEEGNDWDKLKVQKTRFWDLLDPEERTEAGRSFSGVLAWLSRNEPKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCN3</accession>
    <name>D5GCN3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="11">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00000744001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430118">
        <property type="protein sequence ID" value="CAZ82276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838085.1">
        <property type="nucleotide sequence ID" value="XM_002838039.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCN3"/>
    <dbReference type="FunCoup" id="D5GCN3">
        <property type="interactions" value="673"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GCN3"/>
    <dbReference type="GeneID" id="9187897"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000744001"/>
    <dbReference type="eggNOG" id="KOG0760">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015166_3_1_1"/>
    <dbReference type="InParanoid" id="D5GCN3"/>
    <dbReference type="OMA" id="WRPMRGM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015093">
        <property type="term" value="F:ferrous iron transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048250">
        <property type="term" value="P:iron import into the mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000029">
        <property type="entry name" value="Solute carrier family 25 member 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45758:SF4">
        <property type="entry name" value="MITOFERRIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45758">
        <property type="entry name" value="MITOFERRIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="18"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="115"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="206"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ82276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="303" mass="32792" checksum="E79E3298E77BF8FC" modified="2010-06-15" version="1">MADHAPPQDIDYESLGGGSLTSNLVAGAFAGVMEHVVMYPVDAIKTRMQIVNPTPAAMYTGITNAVAQISSTEGVRSLWRGIASVAVGAGPAHAVYFGTYEAVKQKLGGNVGSEHHPFAVATAGACATIASDALMNPFDVIKQRMQVHGSTYESITHCARSVYRNEGFRAFYISYPTTLAMTIPFTAIQFTAYESLAKVLNPTRRYDPFSHCLSGGMAGAVAAAMTTPLDVIKTLLQTRGTSHNSRIRNSKGLFDAAKIIHEREGYRGFFKGLRPRIVTTMPSTAICWTSYEMAKYYLRLNSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5GED5</accession>
    <name>D5GED5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chromatin-remodeling ATPase INO80</fullName>
            <ecNumber evidence="4">3.6.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00006434001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">ATPase component of the INO80 complex which remodels chromatin by shifting nucleosomes and is involved in DNA repair.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the INO80 chromatin-remodeling complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="4">The DBINO region is involved in binding to DNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.-" evidence="4"/>
    <dbReference type="EMBL" id="FN430175">
        <property type="protein sequence ID" value="CAZ82878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838687.1">
        <property type="nucleotide sequence ID" value="XM_002838641.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GED5"/>
    <dbReference type="STRING" id="656061.D5GED5"/>
    <dbReference type="GeneID" id="9185534"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006434001"/>
    <dbReference type="eggNOG" id="KOG0388">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_367293_0_0_1"/>
    <dbReference type="InParanoid" id="D5GED5"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020838">
        <property type="entry name" value="DBINO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050520">
        <property type="entry name" value="INO80/SWR1_helicase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45685:SF2">
        <property type="entry name" value="CHROMATIN-REMODELING ATPASE INO80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45685">
        <property type="entry name" value="HELICASE SRCAP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13892">
        <property type="entry name" value="DBINO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51413">
        <property type="entry name" value="DBINO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DBINO" evidence="6">
        <location>
            <begin position="542"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="393"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="613"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="46"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="63"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="211"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="265"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="393"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="413"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="433"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="489"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="499"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU368001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ82878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="759" mass="85107" checksum="ACAF70537383ADD1" modified="2010-06-15" version="1">MSGSQQHSPYNPNPSPSLPYRASFSPSEQHRPPLPNSAAHYPNPSILPQQTHQQFAPNGRSGPAASLPPLATSAPYVTRAGYYDPVESRPSDMSSGWSQSTTSTTNIAATTYERRHSTQSQTSRDQRYAYGGESIVRGATQSPIHPIDPNSRRSYPLPSPTSASSIHHTTAELRSSMGPGALHPDYAAGPSSPTAKKATTAHRGDAMSISALLSNADEPSTDTTTPKNVPRLTQTTKRRSPDRDSNNTSLAMSRAGLRQAPPAKQATTSASSFRSGDADATDTELSFSNHGGSGGKNGSFRGFRYHDSVNLTDDPDKTDSELDDGGFAQERAEYLKKIRKRRLEVEGRENENRKRRRVVFQNHLHDQFHAHAKSSRERARIQFYEDAMEQVMKREEEDEKERKKEQQRRRRREKAEFEKKSRAEIAAKAAVEAAEREAAEKHVRDEIARQRSAERAARDGLDGALGLGPDMHDSDASGPPMVVVDEGGKKRKPGRKRQPKETKETPEVKEVKELKPIIKEPPVQEVYVPHVSKSYNQIFDQIWKDIARRDIPKVYRVQQTSLATKQSNLKKTAQLAAKEAQRWKLRTNKNVKDTQARAKRCMREMMTFWKRNEREERDSRRRAEKEALEHAKREEENREAKRQARKLNFLISQTELYSHFIGRKIKTDEVERSTDSAGPVRSFDDLDFDAEDDDTLHRVAINNAQHAIQAAQDTARAFNAPGPGDGKTDLGNVNLDEGEMNFQNPTISIEGIELALQSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEF7</accession>
    <name>D5GEF7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00006466001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. ADCK protein kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430175">
        <property type="protein sequence ID" value="CAZ82900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838709.1">
        <property type="nucleotide sequence ID" value="XM_002838663.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEF7"/>
    <dbReference type="FunCoup" id="D5GEF7">
        <property type="interactions" value="376"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GEF7"/>
    <dbReference type="GeneID" id="9181531"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006466001"/>
    <dbReference type="eggNOG" id="KOG1234">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006533_9_2_1"/>
    <dbReference type="InParanoid" id="D5GEF7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd13970">
        <property type="entry name" value="ABC1_ADCK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034646">
        <property type="entry name" value="ADCK3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051409">
        <property type="entry name" value="Atypical_kinase_ADCK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43851">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43851:SF3">
        <property type="entry name" value="COENZYME Q8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ABC1 atypical kinase-like" evidence="6">
        <location>
            <begin position="418"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="47"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="239"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="72"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="102"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="240"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="267"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ82900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="763" mass="83804" checksum="A7486323E053BFC0" modified="2010-06-15" version="1">MASRRLSDMMAVFSASSAVARKHYLIRTQQLNLYASTSSILKSYYRSRDMTPDSPPRDGAIPPSNTSTHSSNKGERDKEYYSDRPTGVSNESLPKDGPELKPQSSKLATPKLRTSLSPEEAKRLQNESETSILSAVAEPPARAETRGIDDDTFYLRHARASPVLSNLPRVKIPRKTEGELESSGEANFDAFYATPKRDGETAEIPEKFVLPEQEGGGVGGLDGELFHSRRARSLLTQLRGAKEKTLEKAAKAPREENTEGKDEDTFYTRTSVDISPDTGTPSPAISSVPKQEKKGIEDLASEISKDVGKTSTAITEQVEEPAFQMSASRVPSSRLGRLFHYGGLAAGMAWGAAGESLRRATGGGDSGSTSGVLLNPPNVERLVKKLSMMRGAALKLGQMMSIQDSKMLPPALQEILTRVQDSADYMPPLQRDSVLESNLGPQWRDLFTEFEEVPMAAASIGQVHAATLSSNGLPVAVKIQYPGVAASISSDLSNLAILMTASRLLPKGLYLDKTIANARVELAWECDYLREAECAKRFATLLQNDTFVFKVPAIIDEACGPQVLTMERMGGIGVTKIISKLTQQERDFIGTQILKLCFREVQEFKFMQTDPNWTNFLWNMGEQKIELLDFGASRAYPESFVQTYSALLKAGARSEESQIRDLSIKLGYLTGLESQTMLRAHVGSILALSEPFSANAPEVYDFSDQTVTDRVRGFIPLMMRERLTPPPEETYSLHRKLSGAFLLCARLGSRVPCRGIFEEVLGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEF8</accession>
    <name>D5GEF8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00006467001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430175">
        <property type="protein sequence ID" value="CAZ82901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838710.1">
        <property type="nucleotide sequence ID" value="XM_002838664.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEF8"/>
    <dbReference type="GeneID" id="9181532"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006467001"/>
    <dbReference type="HOGENOM" id="CLU_2499509_0_0_1"/>
    <dbReference type="InParanoid" id="D5GEF8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ82901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="86" mass="9680" checksum="832D87651CA8E4CD" modified="2010-06-15" version="1">MDHCQSLREVLPDSCIKDIEVLLFRTYPRPPFIPSPFIPSLTLASRTTIGTNRLLNPHNSNILSICHLARDSLQPPRNSKETNTGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEI6</accession>
    <name>D5GEI6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00006509001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430175">
        <property type="protein sequence ID" value="CAZ82929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838738.1">
        <property type="nucleotide sequence ID" value="XM_002838692.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEI6"/>
    <dbReference type="STRING" id="656061.D5GEI6"/>
    <dbReference type="GeneID" id="9181560"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006509001"/>
    <dbReference type="HOGENOM" id="CLU_2607758_0_0_1"/>
    <dbReference type="InParanoid" id="D5GEI6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ82929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="79" mass="8794" checksum="9D27521E22F7AC80" modified="2010-06-15" version="1">MDILGKHRIGFNNLGVQLFADLERDLSLALTKGSSEKADLERAIAEQKELLQERQALVYTPNSPIGVALVIPYPKNYLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEX8</accession>
    <name>D5GEX8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Autophagy-related protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00006635001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Preautophagosomal structure membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="FN430197">
        <property type="protein sequence ID" value="CAZ83071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838880.1">
        <property type="nucleotide sequence ID" value="XM_002838834.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEX8"/>
    <dbReference type="STRING" id="656061.D5GEX8"/>
    <dbReference type="GeneID" id="9188609"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006635001"/>
    <dbReference type="eggNOG" id="KOG0661">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_555724_0_0_1"/>
    <dbReference type="InParanoid" id="D5GEX8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00180">
        <property type="entry name" value="PKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0072">Autophagy</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="146"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="42"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="57"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="78"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="121"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ83071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="491" mass="53730" checksum="6BC9C54B9298F2A0" modified="2010-06-15" version="1">MSNNTPPPSLQYFSGNVNSGNINIGGDVPSDAWARMMGSLPTSMMNDRANNGGPPRASQSQQTGLHVQTPPEVPNPTQSSGFQQSGGFQQSGGFQSGGLGANNVAPGNPPILSGGIIYGEPRGKTPEPAPGEKEAYGAPVREPPAWKLKYKIGSGAFGTVFLEKVKMREMESPELWAVKRIPKTLPNFPAKQYQAEVRNLQTLSKSNHEWFVKFINSYEDGHHVYIAMEYFPLGDMTKTFARGYRWNESDTKVVMEQLLHGLVAMHNDGITHRDLKPENIFLCLRDHRVLRVKIGDFGTSKRILSTTTTTTYMKTTAGTEAYMAPEVDDTSKTKTNRVDIWSLGCILYRMVAGNPLFRSRMEVFRYSLSACSPPAVVGDVGLGVLCVDFLHDVLQPSHEDRPSAEACLRMPWFTSKASGSGYAIGRDLYARLSRIQVENPDIDSFSDVTANWSSGDTPRRSFSAVATPVTGSTGNTLRSPRTFGTTGTIEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFD9</accession>
    <name>D5GFD9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00006847001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430208">
        <property type="protein sequence ID" value="CAZ83232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839041.1">
        <property type="nucleotide sequence ID" value="XM_002838995.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFD9"/>
    <dbReference type="GeneID" id="9188180"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006847001"/>
    <dbReference type="eggNOG" id="ENOG502QUB2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005577_2_1_1"/>
    <dbReference type="InParanoid" id="D5GFD9"/>
    <dbReference type="OMA" id="IWIKKAS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051293">
        <property type="term" value="P:establishment of spindle localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031578">
        <property type="term" value="P:mitotic spindle orientation checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030473">
        <property type="term" value="P:nuclear migration along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013889">
        <property type="entry name" value="Karyogamy_KAR9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37271">
        <property type="entry name" value="KARYOGAMY PROTEIN KAR9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37271:SF1">
        <property type="entry name" value="KARYOGAMY PROTEIN KAR9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08580">
        <property type="entry name" value="KAR9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="597"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="680"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="17"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="599"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="626"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="692"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="715"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="747"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="773"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="804"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="830"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="840"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="853"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="931"/>
            <end position="940"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="940" mass="102120" checksum="298A4F36179A3F76" modified="2010-06-15" version="1">MSKGRLSPSPSPHRKISSASSNGSVSPAAAAITNSTAAPKLSKELPPTPVEDDEDDEEFHTVKGDGASKTGDDDDGSSSDGEGTHNFSSALQSPNNHDGTFNHASIMMTSSPPGSARPDTSYSTWTEDSRSAARFSNDTTLETKPETQTETRYNNSPYMVSSTSLQRRASVFSLSRVSFSAQLSRLTSLSLPLSEDISSRIRQLPTAREACNALISAGDQISRWIDTAKKVLRGLDAEDDVEWAAQGRESLNEVDAAIGKFSGLVNVYVELIDELQNRDDSDEVEKVLVEILKSMEEVMDGWNEVKIILKGVKDQVETAMEWTELWTMILQDIQAELDACQTIVFEEEEKRHRSMMEDTSIVDLDALETIIEETPISALPKVTKTAGEEESSLLGLMARMQPLRVSLDFLPMRLQSFQARAEGIFPSACEDLAGRRNALEKKWKKLDSDVEGLKKELGEDKWVALFRNAGVQTANMMSSVERSLKKLRDAVTIWEESEGRIDSDLALKIQNYEAKKVHYGSAIERALGLITKGVRDRITVNGEIIRLDAAMRARWKTLEDGMADMEQDMNDLGLNVQTLRDSFSSVNSLEVRSYGGSGIITPGSSPASSVVMTSSPGGIERKRSPPSATRSPSGVVNTRPSHQPRASSIPKKSQYARLSSTGSVVAASVRSSLSPITTGMVRVSSTSGTYQTPPTTRRSSSTSSSSGKPVDNRPRWNSSTNTNDSPFGHNFKPLNLTTPSPYRKNPPNANRITSPTLSLPASPGASRIPGPSPLTSASLPGSPLLSGARRSSGGVSLKSPSPAPRLAPAAGGRQHPELRRQASMSQLRYQSRDQARHSSQESITSSTISHSTYVEEKGREREERQCLRDKENAKRSVDGRPASAMASARANRVSMLPRPTTPVSGSGAGKRNSMPPGTTLTQQQQQAAAKADGRPRWRLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFE3</accession>
    <name>D5GFE3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00006853001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430208">
        <property type="protein sequence ID" value="CAZ83236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839045.1">
        <property type="nucleotide sequence ID" value="XM_002838999.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFE3"/>
    <dbReference type="GeneID" id="9188184"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006853001"/>
    <dbReference type="eggNOG" id="ENOG502SDPH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095773_1_1_1"/>
    <dbReference type="InParanoid" id="D5GFE3"/>
    <dbReference type="OMA" id="TEHGPEW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ83236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="157" mass="17451" checksum="4C861294A5CD5B97" modified="2010-06-15" version="1">MSLPSPSPLQTAAKALCDTLSQSPPPSLPAILQHFAETATAHEHGLPQLTPFLGRELPIPDYFTILADSLSFRNMQFKEYIVDEASGKVFVRGTGRFIWTATGEAWDECFVYLLDFIKDAGGMWKVGRYQVWADSGAAYLARIGRLKRVQDGEEELV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFE7</accession>
    <name>D5GFE7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00006859001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430208">
        <property type="protein sequence ID" value="CAZ83240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839049.1">
        <property type="nucleotide sequence ID" value="XM_002839003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFE7"/>
    <dbReference type="GeneID" id="9188188"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006859001"/>
    <dbReference type="eggNOG" id="ENOG502QRIN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020021_1_1_1"/>
    <dbReference type="InParanoid" id="D5GFE7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044159">
        <property type="entry name" value="IQM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31250">
        <property type="entry name" value="IQ DOMAIN-CONTAINING PROTEIN IQM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31250:SF27">
        <property type="entry name" value="IQ DOMAIN-CONTAINING PROTEIN IQM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="253"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="448"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="487"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="284"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="448"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ83240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="551" mass="61632" checksum="90A0E5ECC3556B05" modified="2010-06-15" version="1">MAPGPEQTSPPSAPISSDRSPEEMAKAAVEIQRHYRGHRERRHLKGHGLTPMQRWNDALEEAEYRQVARPVSLERRVELNKQREEGGGGSAALEHGRKPFCLLNGHNMTTPTIPFLRAVTTAIALIAETLKVQKKGRRKKGKRKRGRNVWRGLNILKLWTCNIFWKWLIHRYGSNLRAYHSYWQQQDTKENFFYWLDRGEGREVDLSVCPREQLEKECVRYLSREERQAYLVKIDNEGRLCWAKTGKRIDTSTKYKDTPHGIVPIDSPEAAEEATDGQRYRPGSSSSISSSSSSSSSSSSSSGSDQSTVADRYIDPNMVAHHGPKKIFHVSASTVLNRLLRKTTKKNTWIFVADTSFRLYVGIKQTGTFQHSSFLHGGRISAAGLIKIRDGQLRGLSPLSGHYRPPAANFRRFVHTLKEEGADTSRMSTSKSYAVLLGLETYASLTKGKKKGKSAARKTVGKGEKTGTHAIATGVDISKGAVKGEEAKLKEVEGEERGGKGKEGKGKEGKEKGREGKGVKVEKEGGWWKFVRRLRLPGKHSRTKSLTRSAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFJ8</accession>
    <name>D5GFJ8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00006941001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430209">
        <property type="protein sequence ID" value="CAZ83291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839100.1">
        <property type="nucleotide sequence ID" value="XM_002839054.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFJ8"/>
    <dbReference type="STRING" id="656061.D5GFJ8"/>
    <dbReference type="GeneID" id="9188239"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006941001"/>
    <dbReference type="eggNOG" id="KOG0725">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_1_1"/>
    <dbReference type="InParanoid" id="D5GFJ8"/>
    <dbReference type="OMA" id="AMYKEIR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050085">
        <property type="term" value="F:mannitol 2-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050664">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019594">
        <property type="term" value="P:mannitol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000090">
        <property type="entry name" value="NADP-dependent mannitol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008">
        <property type="entry name" value="BENZIL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008:SF4">
        <property type="entry name" value="CHAIN DEHYDROGENASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G08710)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ83291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="234" mass="25338" checksum="28DBF3C23236F3D3" modified="2010-06-15" version="1">MAEALCQVGCKAIAIMDVLQEHGDEAAETLNRKYNIAAAFYKVDVREEQAVHDVIQSIALDLGSVDVLICSAGIADSNMPAETYDIGRFRRLIDINLTGTFLCAQAAGRVMIEQGTGGSIIFIASMSGHIVNWPQQQSCYNASKAAVIQLGKSLATEWAPHKIRVNSISPGYMDTALNRVPALDAQKKMWCSRTPMNRLGNVDELNNTAIFLASDASTFMTGSDIVVDGGYCVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFL4</accession>
    <name>D5GFL4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00006972001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430209">
        <property type="protein sequence ID" value="CAZ83307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839116.1">
        <property type="nucleotide sequence ID" value="XM_002839070.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFL4"/>
    <dbReference type="GeneID" id="9188255"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006972001"/>
    <dbReference type="HOGENOM" id="CLU_2428666_0_0_1"/>
    <dbReference type="InParanoid" id="D5GFL4"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ83307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="91" mass="9735" checksum="122B5EE566A649D6" modified="2010-06-15" version="1">MLMLRAAYGCGGGRGGRRDTQLCIIYRAALVHAGAAPSAATTLRCGCNTIDEYSSICGVGRDRVGVLIGVLEMGVICYSYYKYSVWLAGYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFP1</accession>
    <name>D5GFP1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00001846001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430211">
        <property type="protein sequence ID" value="CAZ83334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839143.1">
        <property type="nucleotide sequence ID" value="XM_002839097.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFP1"/>
    <dbReference type="GeneID" id="9188282"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001846001"/>
    <dbReference type="HOGENOM" id="CLU_2348209_0_0_1"/>
    <dbReference type="InParanoid" id="D5GFP1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ83334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="97" mass="11250" checksum="E7905B4001B06BF1" modified="2010-06-15" version="1">MFNCSCTTWWGTYYFPSVTFFLHFPHHNFHCFDKQLIIYRDTKAHTHLPSCARDQALAHICIVTPGSSLQFHKRPLTPLLSLWPIENSSSPGINFEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFQ8</accession>
    <name>D5GFQ8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00007011001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430220">
        <property type="protein sequence ID" value="CAZ83351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839160.1">
        <property type="nucleotide sequence ID" value="XM_002839114.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFQ8"/>
    <dbReference type="STRING" id="656061.D5GFQ8"/>
    <dbReference type="GeneID" id="9188299"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007011001"/>
    <dbReference type="eggNOG" id="KOG2171">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1046591_0_0_1"/>
    <dbReference type="InParanoid" id="D5GFQ8"/>
    <dbReference type="OMA" id="VCEISAC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040122">
        <property type="entry name" value="Importin_beta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10527">
        <property type="entry name" value="IMPORTIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="231"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ83351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="266" mass="29396" checksum="D0E37E4E95DBD62A" modified="2010-06-15" version="1">MDVALGLSKALGTQFGEIWKLVGTLLLKYASSSEAVERSTSVGVIADCIKYMEEGCTPYTSQLMKSLLHRLSDEDQETKSNAAYAVGMLCIKSQAVQEVTGNYGAILHKLEPFLQVQNHRMLDNACGCISRMIMAHPDSVPLGDVLPALAGLLPLKEDYEENEPIYKMLVQLYKNSNQIAFGMTQQFVPIIAAVLSPPEEQLTPETRKELIELVQFLFETEPGVLRGYDNLISLAVPFPFLVLISLWISLSVFHLGDRSDISGEVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFS0</accession>
    <name>D5GFS0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="15">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">GSTUM_00007042001</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Mel28</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="CAZ83363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002839126.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFS0"/>
    <dbReference type="STRING" id="656061.D5GFS0"/>
    <dbReference type="GeneID" id="9184285"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007042001"/>
    <dbReference type="eggNOG" id="KOG0519">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_50_4_1"/>
    <dbReference type="InParanoid" id="D5GFS0"/>
    <dbReference type="OMA" id="MHKSHAN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009881">
        <property type="term" value="F:photoreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009584">
        <property type="term" value="P:detection of visible light"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
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        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PAS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016132">
        <property type="entry name" value="Phyto_chromo_attachment"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013515">
        <property type="entry name" value="Phytochrome_cen-reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043150">
        <property type="entry name" value="Phytochrome_PHY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43065:SF10">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43065">
        <property type="entry name" value="SENSOR HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08446">
        <property type="entry name" value="PAS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00360">
        <property type="entry name" value="PHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55781">
        <property type="entry name" value="GAF domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50046">
        <property type="entry name" value="PHYTOCHROME_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0157">Chromophore</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 11" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="1" id="KW-0600">Photoreceptor protein</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Phytochrome chromophore attachment site" evidence="13">
        <location>
            <begin position="173"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="14">
        <location>
            <begin position="527"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="11">
        <location>
            <position position="582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
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            <dbReference type="PROSITE" id="PS50046"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAZ83363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="724" mass="81617" checksum="99AAA01D5CD227B0" modified="2010-06-15" version="1">MVVTGVSGSESIQRCEDEPIHIPRAVQGFGVLIALKGGMEGGLDVKIVSENSEQIIGYSPAALFALGSFCNILSEEQAENLYEHLDFETFELEGNHIYPLFSPAEEETPPPPEDTLNSNPSEQDLLESTLSASRPLRVLMHARKRRGQAAVMEVFNLMSQFQEQLSSASTLAVTELVDPRATKDLYKSLRFPASDIPKQARDLYLLNKVRLLYDRDQVTARLVCKSKDYLDHPLDMTHSYLRAISPIHIKYLKNMAVRASTSVSLTEFDKLWGLISCHSYGQKGMRVSFPLRKMRRLIGETASKNIERLSYASRLQTRKLINTVPTEQNPSGYIIASSDDLLKPFDVDFGLLSIREETKVLGKLEHSRESFAMLEYLRMRKFTSVLTSQNLSVDFPDLNYEPGFSIIAGLLLVPLSVGGQDFIVFFRKGQLWKVNWAGNPYEKSVKEGTKEYLEPRESFKTWSETVTKFFIEVWRQKETALQNSQLTRLLFANASHEVRTPLGAIINYLEIALESPLDNETRDNLMRPHSASKSLIYYEILVAEDNPINSKMGHGVVLTVNGEECADLYSKHGQNFDIVLMDIQASLFDFRSSMPIMDGGTSATRIRQFEGADSDQCIPTRHRINRRVPIFAVSASLAEERRAEYIDFGFDGWILKPVNFGRLKLLLDGIRDPAVRARNAYEPGHWEQGGWLSGTPKDTKLLSPKHGYISPAALKAEDKDQDGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFV1</accession>
    <name>D5GFV1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00007099001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430220">
        <property type="protein sequence ID" value="CAZ83394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFV1"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007099001"/>
    <dbReference type="HOGENOM" id="CLU_2759642_0_0_1"/>
    <dbReference type="InParanoid" id="D5GFV1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ83394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="70" mass="8123" checksum="5B22A319B06F6DBF" modified="2010-06-15" version="1">MEAAVFKALQEAFSPNNGLDWTYEIHELVIVDSSGRESTIPARVVEYLLHARRLGFVRRPVKNQRRSKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFZ2</accession>
    <name>D5GFZ2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA polymerase eta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00001942001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430229">
        <property type="protein sequence ID" value="CAZ83435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839244.1">
        <property type="nucleotide sequence ID" value="XM_002839198.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFZ2"/>
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        <property type="interactions" value="590"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GFZ2"/>
    <dbReference type="GeneID" id="9181783"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001942001"/>
    <dbReference type="eggNOG" id="KOG2095">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012348_7_1_1"/>
    <dbReference type="InParanoid" id="D5GFZ2"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="entry name" value="Polymerase (DNA directed), eta"/>
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    </dbReference>
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        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
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        <property type="entry name" value="DNA_pol_Y-fam_little_finger"/>
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    <dbReference type="InterPro" id="IPR052230">
        <property type="entry name" value="DNA_polymerase_eta"/>
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    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
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        <property type="entry name" value="UBZ3"/>
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    <dbReference type="InterPro" id="IPR001126">
        <property type="entry name" value="UmuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45873">
        <property type="entry name" value="DNA POLYMERASE ETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45873:SF1">
        <property type="entry name" value="DNA POLYMERASE ETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00817">
        <property type="entry name" value="IMS"/>
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        <property type="entry name" value="POLH-Rev1_HhH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18439">
        <property type="entry name" value="zf_UBZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036603">
        <property type="entry name" value="DPol_eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100879">
        <property type="entry name" value="Lesion bypass DNA polymerase (Y-family), little finger domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50173">
        <property type="entry name" value="UMUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51907">
        <property type="entry name" value="ZF_UBZ3"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UmuC" evidence="11">
        <location>
            <begin position="31"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="UBZ3-type" evidence="12">
        <location>
            <begin position="545"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="468"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="582"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="482"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="604"/>
            <end position="617"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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        <source>
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            <dbReference type="ARBA" id="ARBA00022771"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00044975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50173"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51907"/>
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        <source>
            <dbReference type="EMBL" id="CAZ83435.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="627" mass="70531" checksum="37DEF2A77CD4ACDB" modified="2010-06-15" version="1">MEEPIERKSKYTYRHLGQLLRYSVDTPLRVIAHIDLDAFYAQCEMKRLGTDENTPLAVQQWQSLIAVNYPAREFNVSRHSTAVDALKVCPQLKLVHVATWRSGDEHWGYRENPDIVTCKACLDPYRIESKKIMSIFRAACLRVEKASIDESFLDLSVMVHERLLKRFPVLILPPPHNDPAEKLPLPPEGLEIKWARSHLLELAEDCDEQLDWDDVGMGVAAEIVEEVRTSVRKELGYTCSAGIAQNKLLAKLGSGYKKPNQQTIVRIRAAQRFLNTFKFTKLRNLGGKLGERISEEFGTEELSSLLDTPLQALQLKLNDDTATWVYNIIRGIDKSEVNPRTLIKSMLSAKSFRPYITTADAGSKWLTIFISDIYSRMEEEGVMEGKRRPKTMTLNYRSSGGAGKGRSVSIREVTKPALTAVAQDLLKGVVDDTRAWPCAMLSLAVSGFEEREVGNLGIRRFLVSGDAAKDRNSPKSELSGVVEREPKRRRVAEDTGRFFTKELATHSETPNPEDTLPEDAILDPETEEALDNPPSFPLPVAATPRDRPMFLCDRCKTSLPFEDQEEHQDWHFAKDLAREDRTEVMKRIVSPAPPSSSRKPRKGSGSGNGKKGGGGSEKGQRKLIFRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGB7</accession>
    <name>D5GGB7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00002006001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430254">
        <property type="protein sequence ID" value="CAZ83560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002839323.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGB7"/>
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    <dbReference type="GeneID" id="9183158"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002006001"/>
    <dbReference type="eggNOG" id="KOG4668">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1950380_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGB7"/>
    <dbReference type="OMA" id="MSEDPHI"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003954">
        <property type="term" value="F:NADH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015990">
        <property type="term" value="P:electron transport coupled proton transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003945">
        <property type="entry name" value="NU5C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001516">
        <property type="entry name" value="Proton_antipo_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42829">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42829:SF2">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00662">
        <property type="entry name" value="Proton_antipo_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01434">
        <property type="entry name" value="NADHDHGNASE5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="57"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="86"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="NADH-Ubiquinone oxidoreductase (complex I) chain 5 N-terminal" evidence="6">
        <location>
            <begin position="57"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ83560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="129" mass="14218" checksum="F47554520F79F1D8" modified="2010-06-15" version="1">MYLAIITLPLLGSIASGLFGRKIGVTGAQLITSSLVILTTLLAIAAYFEAGFHFDSLTVLIVSCLVHIYSIGYMSHDPRGRHNQRFFSYLSLFTWFMIILVTSENLLLMFVGQPFSAGVGICSYLLVNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGD0</accession>
    <name>D5GGD0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00007319001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430264">
        <property type="protein sequence ID" value="CAZ83573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839382.1">
        <property type="nucleotide sequence ID" value="XM_002839336.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGD0"/>
    <dbReference type="GeneID" id="9183171"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007319001"/>
    <dbReference type="HOGENOM" id="CLU_729957_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGD0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072770" evidence="2">
        <location>
            <begin position="28"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="347"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ83573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="379" mass="42337" checksum="6126171457AC98F5" modified="2010-06-15" version="1">MLSLITLPRGLILLLLLGLTTPHTTRAFPSKVDPELLLDSDKSYETYHEKYKCLEEECYVYYGVDALYEGNCTVILHSPNAWSASVGLGGDLKHPLTLPGTLYLGPTHSYGLRTFDPNDLFLALDAEAKGFGDLGFSSINYMTSVFPAYLLNGIRGKEGRVLWDVTAETMMDDMEGDSTYVYVGGELRYSAPTPPQDDFANQLKLYWPVGWGSGLVKTGKTGRVANTRGVEKYLYSRPPVGRVPPGQPYEMKFGGIVEGETARVNFTVTDYCSRAVCEDWDDRYHFNYTVVFVGINRAGGVGRWPMPVMEIHKDRMLFTDGRTGRLEEVEGVEDREDCGAVCRVFRWLGSISGLYIGVILYGGLCLGLVLFFIRAMFFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGE0</accession>
    <name>D5GGE0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007332001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430264">
        <property type="protein sequence ID" value="CAZ83583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839392.1">
        <property type="nucleotide sequence ID" value="XM_002839346.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGE0"/>
    <dbReference type="FunCoup" id="D5GGE0">
        <property type="interactions" value="33"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GGE0"/>
    <dbReference type="GeneID" id="9183181"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007332001"/>
    <dbReference type="eggNOG" id="ENOG502QT0V">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033921_1_1_1"/>
    <dbReference type="InParanoid" id="D5GGE0"/>
    <dbReference type="OMA" id="VICYINE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03062">
        <property type="entry name" value="TRX_Fd_Sucrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009737">
        <property type="entry name" value="Aim32/Apd1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31902">
        <property type="entry name" value="ACTIN PATCHES DISTAL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31902:SF14">
        <property type="entry name" value="ACTIN PATCHES DISTAL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06999">
        <property type="entry name" value="Suc_Fer-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="312" mass="34723" checksum="7E6561C0D5FCB5E0" modified="2010-06-15" version="1">MPLAALIRRGTSFFNSGKFAGPTTKDTLFPVVSKEVDGEDCDHDCDHCPGYGRAFDKIGIETEGELWGGIKGFANHVIVATGETDWIRDVEDIKGSVMRELKKNSEMLEQGRLMISASNLHPPLEYYSAAEKGKPQPTNVIILPSFTVIENVTPNETPELIRRFINTGPTTSTPLDPPALTPPSSTLKSHPFPHSYLILICSHRRRDARCGISAPILRKEFEKHLRPLNLWRDLTDTRDGGAKVLFINHVGGHKYSANVIIYRKEDGQGIWLARVAPKHIEGIVRFTILQGKVVHPDMIRGGFNRRAGGISW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGE5</accession>
    <name>D5GGE5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00007341001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430264">
        <property type="protein sequence ID" value="CAZ83588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839397.1">
        <property type="nucleotide sequence ID" value="XM_002839351.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGE5"/>
    <dbReference type="GeneID" id="9183186"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007341001"/>
    <dbReference type="eggNOG" id="ENOG502SU5Q">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_597441_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGE5"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="437"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="145"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="202"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="239"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="277"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="329"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ83588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="458" mass="50252" checksum="5E2E7860341AC93F" modified="2010-06-15" version="1">MGVVEEVLRWGLVRVLVGLEGGAGGYRGQAVCSLSDWIAGANTGGGGRTCPSIWEGVYFMGWIWSFVECMFSWRTLRPLHHSSTSCSSTTASPTPKRFLFSRTVGRPLLGKRIRSDSTLGPSEDSSENRGLFGTFYRKRRGIQPTHRDYHHDGEEGAAQFADPAATTGAESGSSRTAYDEPILGISGGKQANVEELIPRLEEVRRGDAGDEEEGRVRAGRNEADSGDGYGDDEESDSAFSSNRSSMDSTTFRAQTPLLSSSLPYDPNQQHPHHESQALLQSSQIPVSYNTMPHLPPSPPHHQSSDHEGLFGFHPPYLAGPSSSTATITRPHRPSPSRRRPTNPEAHHFPSRRSTRKVYGVSINSLPPYLPIMWRTAALLRHLSNALIYAWAPAIVYRGRFEWWGLVILLLLAGKRGWYTVEWFGGGASRVGLAKSSIMALVLAAVVYFLGLGVWGIIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGG3</accession>
    <name>D5GGG3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00007373001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430264">
        <property type="protein sequence ID" value="CAZ83606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGG3"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007373001"/>
    <dbReference type="HOGENOM" id="CLU_2869284_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGG3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ83606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="64" mass="7373" checksum="8CCE7BE84EC07927" modified="2010-06-15" version="1">MDYTPSKKHNDLCFLPQRCSSTPLLCRLHGGNLYSRVNNRRLIVSPRKLILLFIQPCSKQGWGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGX8</accession>
    <name>D5GGX8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007604001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430297">
        <property type="protein sequence ID" value="CAZ83771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGX8"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007604001"/>
    <dbReference type="HOGENOM" id="CLU_2924398_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGX8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="61" mass="7053" checksum="3698DEB9F7F19F22" modified="2010-06-15" version="1">MIVIIVLFMRLGSVEICAGNPLVVCSFIPCFTLFTLFIFSFPFLSSPFLFLFLFLFLFLCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GH41</accession>
    <name>D5GH41_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00007692001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the XPG/RAD2 endonuclease family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430297">
        <property type="protein sequence ID" value="CAZ83834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839643.1">
        <property type="nucleotide sequence ID" value="XM_002839597.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GH41"/>
    <dbReference type="FunCoup" id="D5GH41">
        <property type="interactions" value="339"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GH41"/>
    <dbReference type="GeneID" id="9185088"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007692001"/>
    <dbReference type="eggNOG" id="ENOG502QVHA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_378548_0_0_1"/>
    <dbReference type="InParanoid" id="D5GH41"/>
    <dbReference type="OMA" id="RFYQTKV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09902">
        <property type="entry name" value="H3TH_MKT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09858">
        <property type="entry name" value="PIN_MKT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022039">
        <property type="entry name" value="MKT1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037314">
        <property type="entry name" value="MKT1_H3TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022040">
        <property type="entry name" value="MKT1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006086">
        <property type="entry name" value="XPG-I_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006084">
        <property type="entry name" value="XPG/Rad2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081">
        <property type="entry name" value="FLAP ENDONUCLEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081:SF32">
        <property type="entry name" value="POST-TRANSCRIPTIONAL REGULATOR MKT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12246">
        <property type="entry name" value="MKT1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12247">
        <property type="entry name" value="MKT1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00867">
        <property type="entry name" value="XPG_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00484">
        <property type="entry name" value="XPGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="XPG-I" evidence="3">
        <location>
            <begin position="144"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ83834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="719" mass="80264" checksum="B52D8BB04578F94E" modified="2010-06-15" version="1">MPIRSLDTWATSHNKSQTLPLSVLKDTTIGIEADHYLLRLLTTSPSKEPLVTALGGFPFGLRYAVEGDIEDFQKAGIKPIFVFSGLKIVRNEKPFSVNDDGPAKRSQAWDLYDQGLASEAVEAFGAAGTLQPHEVYKYFIKILHECAVDFQVAPYSAWPQLVYLEKNGFVDSICGNSELFFFDVEKVIVHLNPGQGGSFTWINKQKLISDLQLTNDMFIDSCMLAGSEICRAFPPFEGSPHNAFMSSVELLKRCRNVATVVSTHPEPIQQSGYLDRYRKARAAVKHHAIFTSDGKAEPLDFSEAPSDVHEFIGQRLPEELYYYLSKGVIGPQVLDMLATGELIEVAPFDNGDSDEYKKFLENLNPLRTQALGLLAQPLHRFWFSKEINVYYWFDKNSPRKLIHKEVSVSPAEQTKKWNVRESVFKPELERDGSSIGLAFAISSLKSDSFAAKTIAPKDEVKLLNTKDEILMNTYWRVLQLRGFIDSENHSLTSWGRALEAAISTLDPSDNLDEPVYLGIELLHLGVLKPDNFSPSLSGAPSRGTEEERAYIMLISRVASLVPINHKPIGYTGPLSRNLLGFNSFITKLTRTMRSFAEMALVNLLMNGDADRGERTDWAELGLGIPFIDEVSAGLGIAAKTYLDELSNETGPIPEATRRTQKEKLPLMFAQAVDIEADVDLAFRTWDAIIAAVKSLQRESQLANEVKVFLAADKWLAGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5GH74</accession>
    <name>D5GH74_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="20">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">GSTUM_00007779001</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Component of the PAN1 actin cytoskeleton-regulatory complex required for the internalization of endosomes during actin-coupled endocytosis. The complex links the site of endocytosis to the cell membrane-associated actin cytoskeleton. Mediates uptake of external molecules and vacuolar degradation of plasma membrane proteins. Plays a role in the proper organization of the cell membrane-associated actin cytoskeleton and promotes its destabilization.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the PAN1 actin cytoskeleton-regulatory complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Actin patch</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PAN1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430308">
        <property type="protein sequence ID" value="CAZ83899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839708.1">
        <property type="nucleotide sequence ID" value="XM_002839662.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5GH74">
        <property type="interactions" value="54"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GH74"/>
    <dbReference type="GeneID" id="9182604"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007779001"/>
    <dbReference type="eggNOG" id="KOG0998">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001963_1_0_1"/>
    <dbReference type="InParanoid" id="D5GH74"/>
    <dbReference type="OMA" id="GMPGQWG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
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        <property type="term" value="P:endosomal transport"/>
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    <keyword evidence="9" id="KW-0677">Repeat</keyword>
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        <location>
            <begin position="80"/>
            <end position="168"/>
        </location>
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            <begin position="112"/>
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    <sequence length="1325" mass="143497" checksum="B119F62139815161" modified="2010-06-15" version="1">MPIQYQATGQPGGLQGYPSQQGIPPVPPLPTGLSSFSPQSQQTFSSLPQQQPQQQQQQHKKAKSQTKIPTIRLSFITAADQAKFEQLFKAAVGEGQALSGDKARDILLRSQLSPSDLHQIWQLADTTKSGQLLFPEFALAMYLCNLKRGGKTLPSTLPEVVRNEVSSMVDIISFGVPDSAPLPAPRTNVPSFEVSSAPPQTPQTAISPSQPTGQPQPSNSQLLGMMAQPTGFQPQQTGIPGLQSQRTGFPTMQTQFTGFQPQQTGITSQATGYGQGPGSYGGSIPPVPPIPTGVSSLMPSGMNPLQAQPTGRPGQWGFVNTPASVLQGIDALQERMMPQPGREGGFSTQGLQGNAVIPWSITKVEKQKYDQVFEGWDGLKRGLISGDTAIEVFGQSGLPKDNLMQIWTLADSGNKGSLNKDEFAVAMHLIFRKLNGYEIPTRLPPELIPPSTKKFSESLSTVKSFLQEGRKASALQPQATGVSYLKSRSFHDTATAQERKDGTVYRFDDNQVGYKSSARHRAKGARSPSPAVGGSPSPSPGPEMTLDQLRKRVREKQILLNAIDIKDEDEAENDDILDRRDRRDADDLYRRIRRVQEDIDANPNAAVGGTDLEAERRQLRRQLQNLADRLPDLASKVRKTERGIADAKLSLFGLRDAKAHPGSASVIIGTGPGGAITESDRIKARSKAMMQQRLAALTGKPAASTGDDDEAASRRLAEETQKIKSEQESNDRMTRDVEDSVTEFRKSLEGSLKFAPGESGGISDSERRRWEEALGVEDEVKEFIFDLQRQSHSAKLRREDDRDYRRPLAPPSRTSTPSGSSYSSFKSAEERAAFIKQQAEQKMAERLAALGIKPPARSNIGETAQQRAERERKEREERLRKAEEEEAMRERNLQQRLADEQPKPPAPITSKKPPPPAPRRAKDDNQAKARAEEQARKAEESLRAAEEQALKEEQAAQRAKIEQKEEESRAEEEALLREQEAARARLQALEDKVKQGKLQKQEEKKKRQAALQATKEKEARLAAMRAEIERAREEEERLKRLQMELEDSSSDEEGPEELGGVTPTKESVSEEQFVPAPSPSSTAASPPPPPPPPPPPAPPLPPAVKAPSPPATAVPSVPPTSVGADTNNPSNNPFLKHLGANGDVPALSPAAPASTASAAPSAPSAPSSSGAVPSKNPFYKLNPDQTAEPSPRTFSRRQPEEDDWSVVDGGESSSDDDDDPPNRQGTRALAEMLFSTMAPPRPLSSMGSKETEQPISPSIGGAPPPPPPPPPPPAPPMPSGSSAPVAPPIKVDVGSLLNEISLGRALRKTETKDRSAPASGGRVVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHR9</accession>
    <name>D5GHR9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein uL30m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00008010001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uL30 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430320">
        <property type="protein sequence ID" value="CAZ84030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839839.1">
        <property type="nucleotide sequence ID" value="XM_002839793.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHR9"/>
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        <property type="interactions" value="148"/>
    </dbReference>
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    <dbReference type="KEGG" id="tml:GSTUM_00008010001"/>
    <dbReference type="eggNOG" id="ENOG502S7S3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131047_0_2_1"/>
    <dbReference type="InParanoid" id="D5GHR9"/>
    <dbReference type="OMA" id="FHPAEPQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01658">
        <property type="entry name" value="Ribosomal_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1390.20:FF:000010">
        <property type="entry name" value="Large subunit ribosomal protein L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.20">
        <property type="entry name" value="Ribosomal protein L30, ferredoxin-like fold domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036919">
        <property type="entry name" value="Ribo_uL30_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005996">
        <property type="entry name" value="Ribosomal_uL30_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016082">
        <property type="entry name" value="Ribosomal_uL30_ferredoxin-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01308">
        <property type="entry name" value="rpmD_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15892:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL30M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15892">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00327">
        <property type="entry name" value="Ribosomal_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55129">
        <property type="entry name" value="Ribosomal protein L30p/L7e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL30-like ferredoxin-like fold" evidence="8">
        <location>
            <begin position="4"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ84030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="93" mass="10563" checksum="15A0E089B71C79D9" modified="2010-06-15" version="1">MSWFKITLIRSGIGMTERQNNVLKALGLRHRMKTVYHPVSPDTAGMIMKVKELLTVSEVDKPLTQAEIHAKRQPPKGYYVEKQGVVREDIEGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>D5GI38</accession>
    <name>D5GI38_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00008253001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430323">
        <property type="protein sequence ID" value="CAZ84181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839990.1">
        <property type="nucleotide sequence ID" value="XM_002839944.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GI38"/>
    <dbReference type="STRING" id="656061.D5GI38"/>
    <dbReference type="GeneID" id="9181936"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008253001"/>
    <dbReference type="eggNOG" id="ENOG502S689">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082150_0_0_1"/>
    <dbReference type="InParanoid" id="D5GI38"/>
    <dbReference type="OMA" id="LRCISHP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072168" evidence="2">
        <location>
            <begin position="33"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ84181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="258" mass="27753" checksum="DEAB5B56D9CF7516" modified="2010-06-15" version="1">MHPWDTNTGTIPAHPLPLLLLLLLLLFPPSSSPFFYSSFLCSTVPKLIASSTMPHAEELNGASKANGSTSAQSDPASPSSYKSLSHLKSYPIVNDSISVFENHPLGQRSISLSQSCYSTFLAPITPYLSRAAPYVSRADEFADSNLGKIDSYFPILKEPTDEIKSVVAGAVGYPKKLVGEVYLRGADFAKEGREYVFKVYEDECSKSGSTDGVIPKAKATVTTGLVVSSDIMGSLAAYLGNKKEKAKAVAREKTESEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5GI59</accession>
    <name>D5GI59_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peptide hydrolase</fullName>
            <ecNumber evidence="10">3.4.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00008292001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M28 family. M28A subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M28 family. M28B subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.-.-" evidence="10"/>
    <dbReference type="EMBL" id="FN430323">
        <property type="protein sequence ID" value="CAZ84202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840011.1">
        <property type="nucleotide sequence ID" value="XM_002839965.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GI59"/>
    <dbReference type="FunCoup" id="D5GI59">
        <property type="interactions" value="33"/>
    </dbReference>
    <dbReference type="GeneID" id="9181920"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008292001"/>
    <dbReference type="eggNOG" id="KOG2195">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024336_0_0_1"/>
    <dbReference type="InParanoid" id="D5GI59"/>
    <dbReference type="OMA" id="TTMIRYE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008235">
        <property type="term" value="F:metalloexopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03876">
        <property type="entry name" value="M28_SGAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000054">
        <property type="entry name" value="Peptide hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045175">
        <property type="entry name" value="M28_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041756">
        <property type="entry name" value="M28_SGAP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147">
        <property type="entry name" value="METALLOPEPTIDASE M28 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147:SF26">
        <property type="entry name" value="PEPTIDASE M28 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="8 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 10" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0732">Signal</keyword>
    <keyword evidence="9 10" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005126592" description="Peptide hydrolase" evidence="10">
        <location>
            <begin position="20"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="11">
        <location>
            <begin position="209"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ84202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="463" mass="49197" checksum="AC98D0FCAB4BCBBC" modified="2010-06-15" version="1">MKLSTGIFLIFSLAGAAVATPLAPAPTHNVSLVESKKLRRVLNKKALYEHAKEFQSFADSTPIRNRVAGSAGHNLTVDYIYDTLVATGYYNVEKQPFTYVFSEGNTSFSALSTTYPSEFMTYSPSTGGSTLTAQLVKVANLGCDQSDYPELKGKIALISRGDCEFEGPYVPVAAISGTDGTALVAAIGAGPVKGSLLVNSINEVRYTSNVLATSKGGDQNNIVMSGGHTDSVTAGPGINDNGSGSIGNLEIALQLTKWSVNNAVRFGFWSAEEFGLIGSRYYVETLPEAELAKVALYLNFDMIASPNFGYFLYDGDGSSFNTTGPPGSDAIEHLFEDYFQKLGLPTRASDFDGRSDYAPFMEVGIPIGGIFTGAEGNKTAEEAALWGGEIGVAYDRNYHKAGDNIDNLNLGAWIQNTKAAAHAIATYARSTETIPKGTTSKRVKRDMGHTIPGACGCAPLHVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>D5GI74</accession>
    <name>D5GI74_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Diphthine methyl ester synthase</fullName>
            <ecNumber evidence="4">2.1.1.314</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Diphthamide biosynthesis methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00008315001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">S-adenosyl-L-methionine-dependent methyltransferase that catalyzes four methylations of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine methyl ester. The four successive methylation reactions represent the second step of diphthamide biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation elongation factor 2] + 4 S-adenosyl-L-methionine = diphthine methyl ester-[translation elongation factor 2] + 4 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:42652"/>
            <dbReference type="Rhea" id="RHEA-COMP:9749"/>
            <dbReference type="Rhea" id="RHEA-COMP:10173"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73995"/>
            <dbReference type="ChEBI" id="CHEBI:79005"/>
            <dbReference type="EC" id="2.1.1.314"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the diphthine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.314" evidence="4"/>
    <dbReference type="EMBL" id="FN430323">
        <property type="protein sequence ID" value="CAZ84217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840026.1">
        <property type="nucleotide sequence ID" value="XM_002839980.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GI74"/>
    <dbReference type="FunCoup" id="D5GI74">
        <property type="interactions" value="920"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GI74"/>
    <dbReference type="GeneID" id="9181935"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008315001"/>
    <dbReference type="eggNOG" id="KOG3123">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066040_1_0_1"/>
    <dbReference type="InParanoid" id="D5GI74"/>
    <dbReference type="OMA" id="HNASIMS"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141133">
        <property type="term" value="F:diphthine methyl ester synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd11647">
        <property type="entry name" value="DHP5_DphB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.950.10:FF:000004">
        <property type="entry name" value="Diphthine synthase putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1010.10:FF:000004">
        <property type="entry name" value="Putative diphthine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="entry name" value="Methyltransferase, Cobalt-precorrin-4 Transmethylase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01084">
        <property type="entry name" value="Diphthine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004551">
        <property type="entry name" value="Dphthn_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00522">
        <property type="entry name" value="dph5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10882:SF0">
        <property type="entry name" value="DIPHTHINE METHYL ESTER SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10882">
        <property type="entry name" value="DIPHTHINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036432">
        <property type="entry name" value="Diphthine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="12">
        <location>
            <begin position="1"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="112"/>
            <end position="113"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00072033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00081951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR036432-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ84217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="284" mass="31548" checksum="1D1E4567F7E9FD0C" modified="2010-06-15" version="1">MLYLIGLGLSNEKDITIAGLEIVKKASRVYLEAYTSILMIGKDKLEEFYGRSVILADRVLVESSSDTILSGADTDDVAILVVGDPFGATTHTDLLLRARALGIPHRTIHNASIMNAIGACGLQLYNFGQTVSMVFFTDTWRPSSFYDRVRENREIGLHTLLLLDIKVKEQSLENLIRGRKVYERPRYMSVAECAQQMLEIESEKGEGAYSENSLAIGVSRLGSENQQFISGTLTELSTADLGAPLHSLVLLGKRVHELEIDYIREFSIDKESFDRAFAAEHGKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIB9</accession>
    <name>D5GIB9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008417001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430326">
        <property type="protein sequence ID" value="CAZ84262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840071.1">
        <property type="nucleotide sequence ID" value="XM_002840025.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIB9"/>
    <dbReference type="GeneID" id="9184680"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008417001"/>
    <dbReference type="eggNOG" id="ENOG502TE6M">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_449057_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIB9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="21"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="562" mass="62543" checksum="842FEC7D3EBE1783" modified="2010-06-15" version="1">MRSAISLRGGQSAPPEMSVHLPLHLALLLVPYLDPSDVARARRVCKSWYPVFSSSTLAQATLRVHYPLCREIRTPSRSIDSSVLNHVGKRYAALKSGLPQQELRIPITKTRRNGCIWTFSENLIVAQTRHHPTIQLALVYLDTSGDTRHIRNGRVMISAKGETSVAVKVCRDVLAVERSEERVTNPGRSDWVAVVNFFRLSLEGEDAIVATDISTISTFEFTYIDYSHQIRSDHSGKYYAAYSRAEASRSGGMLCVWDIETGRMVRRVPAASLTGIVMITDVVVAAKHGSEGQQHVVVAGNDKRNNNANRWGRFPGEWGYLFSFKTFTIEDDSKEMEWSGFVGRTKAAAPRESNILATTFKVPRFPHAISPVDAFDTPGYEAEDVGLALWELRDRSGQATSYYYKLSPDAHPKSTTDASLTADLEYRLPEGASASGLPAPFTVKGRTGLSFAMDFTGRNEDFEGTRLMLYQLRIQGFYPSDIPGVMERDWESCLKIGGATRQARELYSPNRNVSYTNMLPAMEVVEDEMAIAFLELTQDGMDLVVAIWDEPYEGEVLGGTVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIF0</accession>
    <name>D5GIF0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008457001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430326">
        <property type="protein sequence ID" value="CAZ84293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840102.1">
        <property type="nucleotide sequence ID" value="XM_002840056.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIF0"/>
    <dbReference type="GeneID" id="9184651"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008457001"/>
    <dbReference type="HOGENOM" id="CLU_2198913_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIF0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="108" mass="12210" checksum="9FE600A9CC41C7AF" modified="2010-06-15" version="1">MEARAIRKCANWLPICGDYRTLIKDIPLGSFLFKIGRLWLAVSYNKSGLGWGRGGLSNCISCRLLKVWRFGVFFALVGLVVITRVGSSPMWLLRKYDTGTRRALMLPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIG2</accession>
    <name>D5GIG2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008486001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430326">
        <property type="protein sequence ID" value="CAZ84305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840114.1">
        <property type="nucleotide sequence ID" value="XM_002840068.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIG2"/>
    <dbReference type="GeneID" id="9184663"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008486001"/>
    <dbReference type="HOGENOM" id="CLU_2905803_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIG2"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="62" mass="6913" checksum="CEB9C462F2752CB9" modified="2010-06-15" version="1">MVRISHRQTASSVAQPCITPSGPTAPQLNQLNRLQIVKISAEGLKGQQAEFVRDTMWPFLQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIK6</accession>
    <name>D5GIK6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008540001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430327">
        <property type="protein sequence ID" value="CAZ84349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840158.1">
        <property type="nucleotide sequence ID" value="XM_002840112.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIK6"/>
    <dbReference type="GeneID" id="9183283"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008540001"/>
    <dbReference type="HOGENOM" id="CLU_2943506_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIK6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="60" mass="6930" checksum="26A53849572E8AF6" modified="2010-06-15" version="1">MGGGLSHFARTVPMERRKDRRRAGWTTRTRPYSRVRYRCAAQVLVQVPVSRTGEIFTIPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIM3</accession>
    <name>D5GIM3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00008560001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430327">
        <property type="protein sequence ID" value="CAZ84366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840175.1">
        <property type="nucleotide sequence ID" value="XM_002840129.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIM3"/>
    <dbReference type="STRING" id="656061.D5GIM3"/>
    <dbReference type="GeneID" id="9183300"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008560001"/>
    <dbReference type="eggNOG" id="KOG0379">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005349_0_1_1"/>
    <dbReference type="InParanoid" id="D5GIM3"/>
    <dbReference type="OMA" id="DMADMEF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43503">
        <property type="entry name" value="MCG48959-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43503:SF2">
        <property type="entry name" value="NEGATIVE REGULATOR OF SPORULATION MDS3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="4">
        <location>
            <begin position="495"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="139"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="532"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="655"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="695"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="18"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="141"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="532"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="695"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="705"/>
            <end position="717"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ84366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="732" mass="78481" checksum="9ADBF85B97B57070" modified="2010-06-15" version="1">MPPTMTASTSSITAPPQNASSSNSNTPRSSTPTTTQPQASQNISSLVATILKTSGREPPPLVGSSTTIIGDTLYAFGGRLLSRTKSQLTCDMYALDLITRSWSKLEARGDIPRPRYFHSVCALGGTKLVCFGGMSPAATSPPQTNGTSGTNGNTATSPGGDSQNDVMVMSDIHIYDILSQTWTYIPTLNPPEGRYAHCATILPTSATFTSTNSVAAPLVGGLGLEGFGGAEMVIVGGQDSANHYIEEINVFNLRSLKWTSTTPLDRSCGAYRSVVAPLGRGLTASSIGAGRNSPYMGPEENPPFPAAANGQSTSQVQTSETNAMLIYSNYNFLDVKLELQIRLPDGTLTERPMNGQFSPPGLRFPNGGVLDNHFVVSGTYLTSSKQEYALWALDLRTLIWSRIDAGQAFAQGSWNRGILWSKRNAFLILGNKNRSLVEDYNHRRINFSHLCMVELEAFGLYENPRMDSRSSLQRSCSVAAQELGRMVLGMKEMADMEFLAVGGERVPVNSRIVARRWGPYFLGLLNASNKELPSPTGKNHNSAATITQQSLSQSAQKSEIQPPTTAQFNPLDRPRTLYLPHTLQTLVTLVHYLYTSTLPPPNHPQSSPQILCSLLQIAQPYQVEGLLEAVVERLHQVLDGRNAAAVFNAAAMAAGGGNGGKREPVENPGGTGDEWRGGVSSVIGLQKRGLRGLMEGRRMRERGRSMGSTGTGTKSSGAQTDQGTFGVGVGAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJ06</accession>
    <name>D5GJ06_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Sorting nexin-4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Autophagy-related protein 24</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">GSTUM_00008766001</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the sorting nexin family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840308.1">
        <property type="nucleotide sequence ID" value="XM_002840262.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJ06"/>
    <dbReference type="FunCoup" id="D5GJ06">
        <property type="interactions" value="486"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJ06"/>
    <dbReference type="GeneID" id="9181840"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008766001"/>
    <dbReference type="eggNOG" id="KOG2273">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027221_2_0_1"/>
    <dbReference type="InParanoid" id="D5GJ06"/>
    <dbReference type="OMA" id="WSLHRFI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07628">
        <property type="entry name" value="BAR_Atg24p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06863">
        <property type="entry name" value="PX_Atg24p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000035">
        <property type="entry name" value="Sorting nexin-4 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000042">
        <property type="entry name" value="Vacuolar targeting protein Atg24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015404">
        <property type="entry name" value="Vps5_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45949">
        <property type="entry name" value="SORTING NEXIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45949:SF2">
        <property type="entry name" value="SORTING NEXIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09325">
        <property type="entry name" value="Vps5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0072">Autophagy</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="8" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PX" evidence="14">
        <location>
            <begin position="52"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="372"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="52"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAZ84499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="458" mass="52021" checksum="919C32CA1CB5371C" modified="2010-06-15" version="1">MDSFNSIQWRTTFDDEEEPSPPPSTSGFPNGEEPQAGRNTDKIDMTGVGNGTLETKVSDPQTENEGTKDAFVSYLVTTNTDFPSFQKPLVSVRRRFTDFVFIWTMLTREFPACAVPPLPGKHKMEYVRGDRFGPDFTNRRANSLSRFLNRLTLHPVLRRTSLLMVFLESPDWNAHMRYRPARGAPNSQGETGGVFEGLTDTFLNAFSKVHKPDRRFIEVRDRADKLDDDLMHVAAIITKVVRREADLEADYSDLSKEFLKLLDLEPELMGPVSSFAAGVQSTSSGLRKLKEHTDQDYLGSLRDLQSYIGALKTLLKAREQKQLDFEALTDYLNRATTERDQIASAPAGTTGLGGPTGFLRSKIEDVRGVDHEQSRRDRVRKLELRIKELTRESENARQVSEAFDEEVIREVTDFERIKSCEMKDTLGGLAAANVEFYKGMIENWEKMLKEVEDGERER</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJA2</accession>
    <name>D5GJA2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cysteine synthase 2</fullName>
            <ecNumber evidence="4">2.5.1.47</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Cysteine synthase-like protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">O-acetylserine (thiol)-lyase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">O-acetylserine sulfhydrylase 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">GSTUM_00008902001</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate</text>
            <dbReference type="Rhea" id="RHEA:14829"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:58340"/>
            <dbReference type="EC" id="2.5.1.47"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion outer membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cysteine synthase/cystathionine beta-synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.47" evidence="4"/>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840404.1">
        <property type="nucleotide sequence ID" value="XM_002840358.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJA2"/>
    <dbReference type="FunCoup" id="D5GJA2">
        <property type="interactions" value="206"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJA2"/>
    <dbReference type="GeneID" id="9185963"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008902001"/>
    <dbReference type="eggNOG" id="KOG1481">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021018_1_0_1"/>
    <dbReference type="InParanoid" id="D5GJA2"/>
    <dbReference type="OMA" id="WMADYGF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004124">
        <property type="term" value="F:cysteine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01561">
        <property type="entry name" value="CBS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000049">
        <property type="entry name" value="Cysteine synthase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000096">
        <property type="entry name" value="Related to cysteine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050214">
        <property type="entry name" value="Cys_Synth/Cystath_Beta-Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001216">
        <property type="entry name" value="P-phosphate_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10314">
        <property type="entry name" value="CYSTATHIONINE BETA-SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00901">
        <property type="entry name" value="CYS_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="18">
        <location>
            <begin position="55"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00072090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00078263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00078545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00079149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAZ84595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="419" mass="45030" checksum="EEA81F1861C8F106" modified="2010-06-15" version="1">MQRYSQTTILLGGIAIGAGAVLVADWLSDRPSETPRERKSPPAKATEDIRNGLEECIGNTPLIRIKCLSEATGCEILGKAEFLEPGGSVKDRVAMKLIETAEAEGLLTPHSGDKVYEGTVGSTGVSLAILCRARGYLAHICMPNDQSAEKSDLLTKLGAEVERVPPAPIVDRAQFVNKARARSEEHTKSPIIPGSGFFADQFENEANWKAHYEGTGPEIFDQCGGKLDCFVAGAGTGGTISGVARYLKPHLPKLKIVLADPQGSGLYNRIRYGVMFDVREREGTRRRQQVDTIIEGIGINRVTANFEAGRPLIDDAIRVGDEEAMAMARFLVEREGLFVGSSSAVNCVAAVRTALQMGPGHRVVTILCDSGTRHLSKFWAKAGNVGGGWKMTLEEVLSAEKTRPSLPVSPSRASPFLGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJC4</accession>
    <name>D5GJC4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00008937001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430330">
        <property type="protein sequence ID" value="CAZ84617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840426.1">
        <property type="nucleotide sequence ID" value="XM_002840380.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJC4"/>
    <dbReference type="FunCoup" id="D5GJC4">
        <property type="interactions" value="115"/>
    </dbReference>
    <dbReference type="GeneID" id="9185985"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008937001"/>
    <dbReference type="eggNOG" id="ENOG502QVDR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_814299_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJC4"/>
    <dbReference type="OMA" id="PSAWISV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028000">
        <property type="entry name" value="Pma1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14610">
        <property type="entry name" value="Psg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072782" evidence="3">
        <location>
            <begin position="21"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="244"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="304"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ84617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="341" mass="36844" checksum="BE84BD65A25894FE" modified="2010-06-15" version="1">MIPSVSLLPVLSLLLQSINAAPHDTDKLVRRSDDASTTPTPTAWVSVFGDGSVTTVTPSVKTDSSGNPTETIDAKPSETASSDGDQSDNVVARCDSSKYELGKTGNVQPWVPFCAPHNGTEWWVDGNYYVTWNPKHWTRNSTVKIILNYANPGRGGKVAKLWETANSFGLINIEPSKDWLVNRTAFDDGNKRTVENQTMYFTIADDSPSGTQPNVETGPTIILTSRPSPAPIDKSPSNRPPVSILGLAVGLPVVFVFVVGTILSLHFCMKSKRTVGPISIGGGRRHFSKRGYSGRAVRRQKAAVAVRSGDYRDEPGEEIAPAGGIPPKRDEWELTSVQGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJJ4</accession>
    <name>D5GJJ4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Large ribosomal subunit protein uL14m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00009041001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uL14 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430332">
        <property type="protein sequence ID" value="CAZ84687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840496.1">
        <property type="nucleotide sequence ID" value="XM_002840450.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJJ4"/>
    <dbReference type="FunCoup" id="D5GJJ4">
        <property type="interactions" value="342"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJJ4"/>
    <dbReference type="GeneID" id="9182688"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009041001"/>
    <dbReference type="eggNOG" id="KOG0901">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095071_2_0_1"/>
    <dbReference type="InParanoid" id="D5GJJ4"/>
    <dbReference type="OMA" id="IVCVVQK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00337">
        <property type="entry name" value="Ribosomal_uL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.150.20:FF:000005">
        <property type="entry name" value="50S ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.150.20">
        <property type="entry name" value="Ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01367">
        <property type="entry name" value="Ribosomal_uL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000218">
        <property type="entry name" value="Ribosomal_uL14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005745">
        <property type="entry name" value="Ribosomal_uL14_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019972">
        <property type="entry name" value="Ribosomal_uL14_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036853">
        <property type="entry name" value="Ribosomal_uL14_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01067">
        <property type="entry name" value="rplN_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761">
        <property type="entry name" value="50S/60S RIBOSOMAL PROTEIN L14/L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL14M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00238">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01374">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50193">
        <property type="entry name" value="Ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00049">
        <property type="entry name" value="RIBOSOMAL_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00040118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ84687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="125" mass="13613" checksum="506FA47EEEE04B9E" modified="2010-06-15" version="1">MLNCIDNSGAALVECVRVLGKKRPATVGDKIVVVIQKQRSLGHDLSAAAQAAVKVRRGDISHAVVVRTKKEYRRADGSYIRFDDNACVLINKSGDPLGTRLSGVVGRELKRKKWSKILSLAPAYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKJ1</accession>
    <name>D5GKJ1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00009588001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
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    </evidence>
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    </evidence>
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        <lineage>
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            <taxon>Pezizomycetes</taxon>
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            <taxon>Tuber</taxon>
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        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
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                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
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            <end position="46"/>
        </location>
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            <end position="317"/>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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</entry>
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            <taxon>Pezizomycotina</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
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                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
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                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
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                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="tml:GSTUM_00009962001"/>
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    </dbReference>
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            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
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            <end position="14"/>
        </location>
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            <end position="68"/>
        </location>
    </feature>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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        </source>
    </evidence>
    <sequence length="121" mass="13880" checksum="A84E312F1F8D06B0" modified="2010-06-15" version="1">MEKGEEEVEAEEAGISDQSVNESVTTTTSLFPNPETQFLNRSIRSPPMGRKEGKYPPIHRTDPKKGKIRKADPEHELFFFLLLSFWEMTREKGTRSDPECIWGFRNSRSLGRSVVTAMSWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GL55</accession>
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            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
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        <lineage>
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            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
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            <taxon>Tuber</taxon>
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    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
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                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
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                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
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                <person name="Viscomi A.R."/>
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                <person name="Zampieri E."/>
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                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
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                <person name="Wincker P."/>
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            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <text evidence="1">Belongs to the HIT family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="OMA" id="HVHINPI"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140932">
        <property type="term" value="F:5'-(N(7)-methyl 5'-triphosphoguanosine)-[mRNA] diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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        <property type="entry name" value="Scavenger mRNA decapping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.40">
        <property type="entry name" value="Scavenger mRNA decapping enzyme, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008594">
        <property type="entry name" value="DcpS/DCS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011145">
        <property type="entry name" value="Scavenger_mRNA_decap_enz_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12978">
        <property type="entry name" value="HISTIDINE TRIAD HIT PROTEIN MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12978:SF0">
        <property type="entry name" value="M7GPPPX DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05652">
        <property type="entry name" value="DcpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11969">
        <property type="entry name" value="DcpS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028973">
        <property type="entry name" value="Scavenger_mRNA_decap_enz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102860">
        <property type="entry name" value="mRNA decapping enzyme DcpS N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="244"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="235"/>
            <end position="246"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR028973-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR028973-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ85248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="315" mass="36222" checksum="0D7C2F1429DF53B1" modified="2010-06-15" version="1">MSVSAEEEARLRRFNFTRILNQDTGTKTVNLLGEIESTPAILIAEKTAFNVTGAHLTKFSHDLHLPKVTLIARNDIYHSFMASQNSEYGEVKLTLIFPATETHIQKYTHQQLRMVVETPEIYRELVKPYVEEKRGSGRLDWVHNILEHKAEKESIIVEDTDDVEGFILLPDLKWDRKTMASMYTMAIVRRRDITSIRDLKKMHVPWLKNLRRRIPEVICEKYSGIEPDQIKLYVHYQPSYYHFHLHAISISHDSGIGQATGKAILLGNIISRLESLGGDEEAGMESVELTYFLGEESELWQRVFKGINGKGKAKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLH7</accession>
    <name>D5GLH7_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Protein-S-isoprenylcysteine O-methyltransferase</fullName>
            <ecNumber evidence="3 10">2.1.1.100</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00010209001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[protein]-C-terminal S-[(2E,6E)-farnesyl]-L-cysteine + S-adenosyl-L-methionine = [protein]-C-terminal S-[(2E,6E)-farnesyl]-L-cysteine methyl ester + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:21672"/>
            <dbReference type="Rhea" id="RHEA-COMP:12125"/>
            <dbReference type="Rhea" id="RHEA-COMP:12126"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90510"/>
            <dbReference type="ChEBI" id="CHEBI:90511"/>
            <dbReference type="EC" id="2.1.1.100"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Endoplasmic reticulum membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the class VI-like SAM-binding methyltransferase superfamily. Isoprenylcysteine carboxyl methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.100" evidence="3 10"/>
    <dbReference type="EMBL" id="FN430349">
        <property type="protein sequence ID" value="CAZ85370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841179.1">
        <property type="nucleotide sequence ID" value="XM_002841133.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5GLH7">
        <property type="interactions" value="451"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GLH7"/>
    <dbReference type="GeneID" id="9186114"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010209001"/>
    <dbReference type="eggNOG" id="KOG2628">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065200_0_0_1"/>
    <dbReference type="InParanoid" id="D5GLH7"/>
    <dbReference type="OMA" id="IKREEAY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004671">
        <property type="term" value="F:protein C-terminal S-isoprenylcysteine carboxyl O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007269">
        <property type="entry name" value="ICMT_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025770">
        <property type="entry name" value="PPMT_MeTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12714">
        <property type="entry name" value="PROTEIN-S ISOPRENYLCYSTEINE O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12714:SF9">
        <property type="entry name" value="PROTEIN-S-ISOPRENYLCYSTEINE O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04140">
        <property type="entry name" value="ICMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51564">
        <property type="entry name" value="SAM_ICMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="183"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ85370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="245" mass="28041" checksum="6C3081BFBEF95BDF" modified="2010-06-15" version="1">MSTFPPRRAPSATSPRSRSSLPTSPPSTRRVYTPKPFDIPGYFYPSGIYSLSAIAARAFLLGSAFASSLLLAYLFRNAYPQLPLFITALSLFHFLEFWTTAAYNTRKAKVSSYLLTNNGMAYNLAHTAAFYXXXXXWGVARSLAMVHAGTNFSHLVAYRKEEDHVLVKSGIYRYLRHPSYFGFFWWGLGTQIMLGNPVCFMGYSLVLWKFFSSRIRGEEELLVEFFEDEYREYRARTPTCIPFIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLI0</accession>
    <name>D5GLI0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Inactive metallocarboxypeptidase ECM14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Inactive metallocarboxypeptidase ecm14</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">GSTUM_00010216001</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Inactive carboxypeptidase that may play a role in cell wall organization and biogenesis.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Vacuole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the peptidase M14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="FN430349">
        <property type="protein sequence ID" value="CAZ85373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841182.1">
        <property type="nucleotide sequence ID" value="XM_002841136.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLI0"/>
    <dbReference type="FunCoup" id="D5GLI0">
        <property type="interactions" value="798"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GLI0"/>
    <dbReference type="GeneID" id="9186117"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010216001"/>
    <dbReference type="eggNOG" id="KOG2650">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019326_1_0_1"/>
    <dbReference type="InParanoid" id="D5GLI0"/>
    <dbReference type="OMA" id="WFYHQLH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03860">
        <property type="entry name" value="M14_CP_A-B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000060">
        <property type="entry name" value="Putative metallocarboxypeptidase ecm14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057246">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705:SF147">
        <property type="entry name" value="INACTIVE METALLOCARBOXYPEPTIDASE ECM14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705">
        <property type="entry name" value="PROTEASE FAMILY M14 CARBOXYPEPTIDASE A,B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00765">
        <property type="entry name" value="CRBOXYPTASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00631">
        <property type="entry name" value="Zn_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00132">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 16" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0926">Vacuole</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072421" description="Inactive metallocarboxypeptidase ECM14" evidence="16">
        <location>
            <begin position="26"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M14" evidence="17">
        <location>
            <begin position="202"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00026187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00026213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAZ85373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="532" mass="59824" checksum="97F1A8320B14024B" modified="2010-06-15" version="1">MHYGIHLLTPAVAIVGLVIQCGTNAAIIPDSLGRRAPVEPRSVHTVQEFPNPADSAPEPPRWSSRIRDSIIGRLFGDTNRHGRRKENQRYLSETAKLASQYASDVVLRFTLKTPEEMKALAEASNILLLDVWSTTKTHADIRIAEADIPPLLDLLPASLQHSHVRLMHDLSQTARDTYPGASTASRYYGQTLDGVQSIFFQDYQPHDVIQAWMNLLRSLFPSLVEIVSIGKSYEGRDIQAIRVGNPRADGMKRKTIIVTGAVHAREWISVSTVSYLAYSLITGYGRNKDRLSDTLVDEFDWYFIPTLNIDGYVYTWEGDRLWRKNRQPTTLAFCKGIDLDRAYNFHFDGSSQSQSNPCSDMFPGASPFEATEAKAFSDWTYNITREGAEIVGLLDFHSYSQQILYPYSYSCDSMPPDLENLEELGFGLAKAIRLVSGEHYEVTSACEGTGFAKYQGTTGGGSMMDFFYAEDRIPYSYQIKLRDTGSYGFLLPRDHILPAGEESMNLLKHFGQFILDDRSPVQRHGDLKKAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLQ5</accession>
    <name>D5GLQ5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00010320001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430350">
        <property type="protein sequence ID" value="CAZ85448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841257.1">
        <property type="nucleotide sequence ID" value="XM_002841211.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLQ5"/>
    <dbReference type="STRING" id="656061.D5GLQ5"/>
    <dbReference type="GeneID" id="9182751"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010320001"/>
    <dbReference type="eggNOG" id="ENOG502QUU6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015630_1_1_1"/>
    <dbReference type="InParanoid" id="D5GLQ5"/>
    <dbReference type="OMA" id="HHRGAKN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110031">
        <property type="term" value="P:negative regulation of G2/MI transition of meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050339">
        <property type="entry name" value="CC_SR_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042:SF190">
        <property type="entry name" value="MITOSIS INHIBITOR PROTEIN KINASE MIK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="122"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="388"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="465"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="586"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="404"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="598"/>
            <end position="620"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ85448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="620" mass="68286" checksum="F42725BB34F9B0ED" modified="2010-06-15" version="1">MATLTRADPHRADLFHSMYGYSSSSVALPPPVLLTSTWWDEQKIRETATPWFVRQALRSSSSKKRLDEQLQFGEGLTESTYLDWILEHTRKFFLILVELNCAEMIFEIIDGSWDDEDLPLSLQTVERLGLGSAGVEKKFAKKQYAYLVRELVPGETVEYSDEEVVPLEVISRRVPTTSSGATKGGLVEKVYFPRQREVYYSRRRVLLSQEGDEDSGVGGNKMSPASFFEEISRLGSIDHPHIVSIFASYTQGDYGFLVLSPCIDLTLKSFLHSTPPAWKSQPKDKRRRIIFNWLHCLADALAHLHEKGISHGDIKPSSIIIEATTYKIFFADTGDLKRLDSSPTSAVGPSSFLPRKFNTSPVSADVEGYEYGAPELWQRILALHGSPSQPTNTVFSGRSYRKASTQSTNSNSSEETPSPSSTNISVGHWSSATPAHPATSDVFSLGCIYLDVLSFKQKRKTTAFISHRSAKNRRPRESGPPDASFHSNLGQVDSWIGGLLKETKKKPDEALVEALGITRRMLLRNPEARPGGRRVADEFYTVVSGACEGTEAPCCGVHNIVGGNAEGEVFGGWGIESLMLKTQTSRGSAGSEGLLSKRSTVGTRASGSSGSVGSRRWMAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>D5GM06</accession>
    <name>D5GM06_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00010345001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430351">
        <property type="protein sequence ID" value="CAZ85468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841277.1">
        <property type="nucleotide sequence ID" value="XM_002841231.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GM06"/>
    <dbReference type="STRING" id="656061.D5GM06"/>
    <dbReference type="GeneID" id="9182771"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010345001"/>
    <dbReference type="eggNOG" id="KOG4711">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001788_0_1_1"/>
    <dbReference type="InParanoid" id="D5GM06"/>
    <dbReference type="OMA" id="RFPKKQY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018823">
        <property type="entry name" value="ArAE_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018820">
        <property type="entry name" value="BRE4-related_DUF2421"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049453">
        <property type="entry name" value="Memb_transporter_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37994">
        <property type="entry name" value="ARAE_2_N DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37994:SF4">
        <property type="entry name" value="ER TRANSPORTER 6TM N-TERMINAL DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10337">
        <property type="entry name" value="ArAE_2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10334">
        <property type="entry name" value="BRE4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13515">
        <property type="entry name" value="FUSC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="52"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="168"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="571"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="643"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="689"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="domain" description="Putative ER transporter 6TM N-terminal" evidence="7">
        <location>
            <begin position="49"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="Integral membrane bound transporter" evidence="8">
        <location>
            <begin position="565"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2421" evidence="6">
        <location>
            <begin position="706"/>
            <end position="868"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF10334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF10337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ85468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="944" mass="103927" checksum="DD02F306D733E417" modified="2010-06-15" version="1">MAHPKEYSLSGLWGKLGLDVPTLVLMAKGALPSVIFTAAYQATPVAKTYSTLGYVVSIIAALSLCILPQAKLVQLTVQNSIAVALAAATSLLGVWTATRARAHYPSPPRQRYNASASAVSAAWLFVVIWLANLLKARIPSLALPVIMYTIFVSIIYTNGGLYTMPQAINAVVLMLKCFFTGYGLAFAVGMIVFPVNCRQIWWKVFAGYLLTSKQLLVEQKNFLRQHETMGTEEGKKSAAALAALEKAHADLFTKLSTELPMAKREIALGRYLTSDLQEAYRLVRATLLPLQGMTVILDIFNRAPRMDSEEDEKCPIPREEQLPEVMKTLHEPYERIVGMCGEAIEHVLLTMKLKSPPKKPGGDEEAPSMEPGEEGFCQTLEEKVQEFYNTRAGDVRTYFTPSKAAKTQDTAEGISAEVYMDEKRRSELYLVLFMEYLLYNAAKSLLGLARFAEEKKQQGVIDSTRLVYPTAKRLKKWLRSMASDCNDDIVDVSDVCAVRVPGLGGIAPKKDPEHLPARNSLQKLGNLIAVFTEGISSSESGFGLRVACATLAVALPAYFESSYIFFTEQRLVWGLIVIAIGMSPTSGASLIGFVGRVAGTVIATVLALINWYIVDGITPGVISFLWFFVAIEFYFVIKHPTLIQSTLTALVTHIIIVCYELQVRKIGNEVSARGGQPVLSINEIGPHRLLVTIGGLCVAMIFTIFPYPTTTRSLIRTSVSSSLFLLARTYSITLSLLQLRIHGTAGDESDKNSPGRRYEKARVKLLTESFLANDSLRSQIEFTGYEPSLRGRFPREQYDGVLEGVRNITSYLSIISYASRTFSMGGEWAEDLSTLLRNVDTQMDKITSLLCLLSASLASGNPLPPSLSLPAPFRLARAMEERDGGVRLDHLVEPGFVAFAVVQVAIGVMRDDLVRVIGMVEELVGRVEFPEERSSLDDLKVEVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>D5GM12</accession>
    <name>D5GM12_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00010478001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Component of the NuA4 histone acetyltransferase complex.</text>
    </comment>
    <dbReference type="EMBL" id="FN430351">
        <property type="protein sequence ID" value="CAZ85555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841364.1">
        <property type="nucleotide sequence ID" value="XM_002841318.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GM12"/>
    <dbReference type="STRING" id="656061.D5GM12"/>
    <dbReference type="GeneID" id="9187335"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010478001"/>
    <dbReference type="eggNOG" id="ENOG502SISH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_353808_0_0_1"/>
    <dbReference type="InParanoid" id="D5GM12"/>
    <dbReference type="OMA" id="IKHHIVP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13037">
        <property type="entry name" value="FORMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13037:SF24">
        <property type="entry name" value="POLYCOMB PROTEIN PCL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0945">Host-virus interaction</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="633"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="687"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="643"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="702"/>
            <end position="721"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ85555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="794" mass="87366" checksum="7F65A1917A0FAB05" modified="2010-06-15" version="1">MPVLEMRPSISDEEMGQKLPIHDQLSPLEVMLAVYTPILECLVKHLPTPSKLKLAQTSTYLRHLLLRYPPFYSHFDFRLSAYEAPGFDTYHLGTIYNLDKFISSLSLETRITSLTLDWTAVTGLFLFGPLMTRCAGTLEHLSVRGCRKVSIKHHIVPYLVYQDIVEPHSVSTPIPPKRALKSLYVYKARGVRRKPFLIDRKPADGDEPSRYLTTLAEKLGIWTDLGLCPTPKLRCPRRKEILRRGKEKYCVPFDKRWRVQDPEASQGNPTLALLSPHELQQRRKWEETEGLFTCDNCEARILDRCEACVYQMTCSNCKKAICHSCAYDRPAGPIAPAETVPNGAPGVLPPLMAPEGPWPNPTTAGISAMATMAPQIGVNANGNGNGNGPQFQVVLATLIQLDLPVPGAPTNIPLAPTFYQPTVPSATETNPVVHFLQPCCQTVTPNSADILCGSCMTSISWAHCDCCGKQICIKHELSGSRRCRGGCEKVFCSSSDAPSAPIGCGDPAEGKAGIKSCLTCGNDVCADCRLDSLHAPPSSDDGTESEVEEAALEKCACDCKVCQDNFYCTDCWPKKTTKCETRHTKFDHKVRGIINHRSIDPAKDINLYLVTFVDVPDKKWVTRARIEEDYGSDTRILSAYSPTDPPPPPPTPELTDPTTEPEPPQEDEILVGEKEWIMEKILGKAIHPCPPLPTATSSSPAPASPAPLPPRPSAAPPPPPDQQTEIYQIKWLGRSTSEATWEPKSGFAVNSREHVLMESYDIEGPISEHRGERDFGVIGEHCDITAMAGGGPFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMA4</accession>
    <name>D5GMA4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00010624001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the prokaryotic/mitochondrial release factor family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430354">
        <property type="protein sequence ID" value="CAZ85647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841456.1">
        <property type="nucleotide sequence ID" value="XM_002841410.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMA4"/>
    <dbReference type="FunCoup" id="D5GMA4">
        <property type="interactions" value="49"/>
    </dbReference>
    <dbReference type="GeneID" id="9183226"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010624001"/>
    <dbReference type="eggNOG" id="KOG2726">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089470_1_3_1"/>
    <dbReference type="InParanoid" id="D5GMA4"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003747">
        <property type="term" value="F:translation release factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052405">
        <property type="entry name" value="Mito_Transl_Release_Factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000352">
        <property type="entry name" value="Pep_chain_release_fac_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045853">
        <property type="entry name" value="Pep_chain_release_fac_I_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46203:SF1">
        <property type="entry name" value="MITOCHONDRIAL TRANSLATION RELEASE FACTOR IN RESCUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46203">
        <property type="entry name" value="PROBABLE PEPTIDE CHAIN RELEASE FACTOR C12ORF65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00472">
        <property type="entry name" value="RF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75620">
        <property type="entry name" value="Release factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Prokaryotic-type class I peptide chain release factors" evidence="6">
        <location>
            <begin position="91"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="138"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="151"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="166"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ85647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="237" mass="25619" checksum="32F9DC73C3B9A4AD" modified="2010-06-15" version="1">MILQKLSSRAPILLLPLLHPPRIPVRPIRAIALTATNFGKPMPPRPSVKEEEIEESFLKGSGPGGQKSESQYPLHCMLLRTHVTPGNISAVNKTSSAVQLKHLPTGIVVKSQATRSRSQNRKIARKILAEKLEFMERGTESRVGALAERDRRRKASKTKKAKRKYAKLEADKGASDGEGEAGSGEEGHIEGGEGESADEQVDRAVGPKLGTSEPRSASAIEEGTATIPIPVNDGLKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMI1</accession>
    <name>D5GMI1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00010738001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430356">
        <property type="protein sequence ID" value="CAZ85724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841533.1">
        <property type="nucleotide sequence ID" value="XM_002841487.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMI1"/>
    <dbReference type="STRING" id="656061.D5GMI1"/>
    <dbReference type="GeneID" id="9182150"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010738001"/>
    <dbReference type="eggNOG" id="KOG1657">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045277_7_3_1"/>
    <dbReference type="InParanoid" id="D5GMI1"/>
    <dbReference type="OMA" id="DIVQCSG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008623">
        <property type="term" value="C:CHRAC"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23645">
        <property type="entry name" value="HFD_Dpb3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050568">
        <property type="entry name" value="Transcr_DNA_Rep_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252:SF54">
        <property type="entry name" value="CHROMATIN ACCESSIBILITY COMPLEX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252">
        <property type="entry name" value="HISTONE-LIKE TRANSCRIPTION FACTOR CCAAT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="4">
        <location>
            <begin position="20"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="128"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="154"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ85724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="170" mass="18645" checksum="2BE699975AE0A35E" modified="2010-06-15" version="1">MPYNTSAIPPSEEVTGHSALPLSRVKKIIRLDDDVNGCSNNAAFLVTIAAEMFVQYLAEQGLKMTYGDRKQRKTMQYKDLATAVARVENLEFLADVIPPTVPYKQVRERKNNKTAKDTETGQLTLNGQRILPIGSSNGRKKSSAESDTIAVASGRERGVNGDKGNEIEMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMJ1</accession>
    <name>D5GMJ1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00010750001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430356">
        <property type="protein sequence ID" value="CAZ85734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841543.1">
        <property type="nucleotide sequence ID" value="XM_002841497.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMJ1"/>
    <dbReference type="STRING" id="656061.D5GMJ1"/>
    <dbReference type="GeneID" id="9182160"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010750001"/>
    <dbReference type="eggNOG" id="KOG1200">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_3_1"/>
    <dbReference type="InParanoid" id="D5GMJ1"/>
    <dbReference type="OMA" id="TCMITGG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000173">
        <property type="entry name" value="3-oxoacyl-[acyl-carrier protein] reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760:SF133">
        <property type="entry name" value="3-OXOACYL-[ACYL-CARRIER-PROTEIN] REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASES/REDUCTASES FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketoreductase" evidence="5">
        <location>
            <begin position="9"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ85734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="244" mass="25171" checksum="F428D42F4DCF2579" modified="2010-06-15" version="1">MMTNSLSKKLVLVTGASRGIGLSISQKFAHTGANLILISRTKSHLDAALSTLPPGGEHQCIAGDVGDPELWEEVRGGCGKKLDVLVNAAGITHYSLLMATKSETIQEVIRTNLVGSVLGCRAVLKGMIRARSGCIINISSVLGIKAGMGSVAYAASKAGVIGLTRSLAAEVGSSGVRVNAILPGYIDTDMTGGMAEGARRDALEFTPVKRMGTVEEVADAAMFLVLNEFANGSQVVLDGGLSAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>D5GML8</accession>
    <name>D5GML8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00010804001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430357">
        <property type="protein sequence ID" value="CAZ85761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841570.1">
        <property type="nucleotide sequence ID" value="XM_002841524.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GML8"/>
    <dbReference type="STRING" id="656061.D5GML8"/>
    <dbReference type="GeneID" id="9182187"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010804001"/>
    <dbReference type="eggNOG" id="ENOG502SK85">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039952_0_0_1"/>
    <dbReference type="InParanoid" id="D5GML8"/>
    <dbReference type="OMA" id="KACEEYM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="263" mass="30212" checksum="3FDAF6A820ABAC6B" modified="2010-06-15" version="1">MPNLYSYVPGGFTQQQPEYFPAPTPGPQSPPPRERTRSRVHSHRREATWSEPSPRSTKPSMPSRSNTDPAAIDADEIRRKAASLGIPADYSLKNWDPNERPVILLGSVFDASSLGEWIFNWTKYHHGRSHDATKTAGGLWGLLIELSSKLKRSREFYHQIKAKENREILDDFMESGERLWSKLKDLLKACEEYMWRGARGVASPRPEEGKTVQMGKKSGAEFVDAMFNGVKEWERTERLMRGMVMWCQRFEVNCEDILRRPDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMY3</accession>
    <name>D5GMY3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00011022001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C48 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430361">
        <property type="protein sequence ID" value="CAZ85896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841705.1">
        <property type="nucleotide sequence ID" value="XM_002841659.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMY3"/>
    <dbReference type="STRING" id="656061.D5GMY3"/>
    <dbReference type="GeneID" id="9183464"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011022001"/>
    <dbReference type="eggNOG" id="KOG3246">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043678_1_1_1"/>
    <dbReference type="InParanoid" id="D5GMY3"/>
    <dbReference type="OMA" id="GFYFEYL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019784">
        <property type="term" value="F:deNEDDylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000338">
        <property type="term" value="P:protein deneddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.395.10:FF:000008">
        <property type="entry name" value="Ulp1 protease family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044613">
        <property type="entry name" value="Nep1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46468">
        <property type="entry name" value="SENTRIN-SPECIFIC PROTEASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46468:SF1">
        <property type="entry name" value="SENTRIN-SPECIFIC PROTEASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="6">
        <location>
            <begin position="80"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="279"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="19"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="292"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ85896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="308" mass="34544" checksum="864D04BF7228EC9C" modified="2010-06-15" version="1">MPFRDAIREMSGFHRDEVTTSNPPTTSSAPTSSSSPPPPRSSGGGRGMMDRMADAGGNLRLRRNEMKLRPEDPYLSYYDVCVTKEDIDCLKGDWLTDNNISFWEEYLEHEELKSPNNKVMLLRPSMVFLLKNTKDPLTLESALPDVKKASHIFLPINDCRNPSIPEGGTHWSLLVVGVSDRVAFHYDSLSPANCGEAREVCKKLGVLLGFSLQFFDLEDTPQQDNGSDCGVHVCWAMKHLLVKRLLAVEREKAVQMTLRGKRVDASGYRKEMFKICEGLRKKASRSTSPRTSKHDGKDSPPRIGDDQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GN21</accession>
    <name>D5GN21_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00011054001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430362">
        <property type="protein sequence ID" value="CAZ85914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841723.1">
        <property type="nucleotide sequence ID" value="XM_002841677.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GN21"/>
    <dbReference type="GeneID" id="9184767"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011054001"/>
    <dbReference type="HOGENOM" id="CLU_2639895_0_0_1"/>
    <dbReference type="InParanoid" id="D5GN21"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ85914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="77" mass="8412" checksum="198B3516B0F1260D" modified="2010-06-15" version="1">MSLSFTAFTNTDTVIHSCSSGRRKIQKAISAHTPPLITLITLHVPVFWNHFSGEADSKKGGIEWGGDLALEDLVLFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5GN58</accession>
    <name>D5GN58_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cytochrome b-c1 complex subunit 8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Complex III subunit 8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00011100001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The complex plays an important role in the uptake of multiple carbon sources present in different host niches.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII), a multisubunit enzyme composed of 3 respiratory subunits cytochrome b, cytochrome c1 and Rieske protein, 2 core protein subunits, and additional low-molecular weight protein subunits. The complex exists as an obligatory dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with cytochrome c oxidase (complex IV, CIV).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Mitochondrion inner membrane</location>
            <topology evidence="1 11">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the UQCRQ/QCR8 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430362">
        <property type="protein sequence ID" value="CAZ85951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841760.1">
        <property type="nucleotide sequence ID" value="XM_002841714.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GN58"/>
    <dbReference type="FunCoup" id="D5GN58">
        <property type="interactions" value="70"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GN58"/>
    <dbReference type="GeneID" id="9185877"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011100001"/>
    <dbReference type="eggNOG" id="KOG4116">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_156007_0_1_1"/>
    <dbReference type="InParanoid" id="D5GN58"/>
    <dbReference type="OMA" id="QWAIERN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045275">
        <property type="term" value="C:respiratory chain complex III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.210:FF:000001">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.210">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004205">
        <property type="entry name" value="Cyt_bc1_su8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036642">
        <property type="entry name" value="Cyt_bc1_su8_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12119:SF2">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12119">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME C REDUCTASE COMPLEX UBIQUINONE-BINDING PROTEIN QP-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02939">
        <property type="entry name" value="UcrQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81508">
        <property type="entry name" value="Ubiquinone-binding protein QP-C of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ85951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="96" mass="10743" checksum="E681FCB3577723D5" modified="2010-06-15" version="1">MGGGEKVPGQYMGWWGSLGSPPQKGITTYTLSANRQRPLAGTMHAAVFNSWRRFKGQVLYVAPPFVVAYFVMDWAVKENHFLNSKEGRKLHGAEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNC2</accession>
    <name>D5GNC2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00011218001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430363">
        <property type="protein sequence ID" value="CAZ86015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841824.1">
        <property type="nucleotide sequence ID" value="XM_002841778.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNC2"/>
    <dbReference type="FunCoup" id="D5GNC2">
        <property type="interactions" value="84"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GNC2"/>
    <dbReference type="GeneID" id="9182215"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011218001"/>
    <dbReference type="eggNOG" id="ENOG502QR7R">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045011_11_1_1"/>
    <dbReference type="InParanoid" id="D5GNC2"/>
    <dbReference type="OMA" id="QTYMFKE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033883">
        <property type="term" value="F:pyridoxal phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01427">
        <property type="entry name" value="HAD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43885">
        <property type="entry name" value="HALOACID DEHALOGENASE-LIKE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43885:SF1">
        <property type="entry name" value="SUPERFAMILY HYDROLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G13290)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ86015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="236" mass="25986" checksum="AB788F78BE2E1CC1" modified="2010-06-15" version="1">MATPARLRFAPLDTSKALSAEAPKLQGIVFDVDGTLCEPQTWMFARMRAALGIAKSVDILDHVCSLAIEKQSAAMESIRNVEKEAMLKMIPQKGLIPLMEYLDKRCIRKAICTRNFETPVNYLISNFMQGHVFSPIVTREFRPPKPDPAGILHISKSWGLGDGGERLIMVGDSADDITAGRRAGSATVLLANDVNRSLVDHENTDCVVESLEDLIPILENGFTVVDRTVSNPQNEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNH4</accession>
    <name>D5GNH4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00011292001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430364">
        <property type="protein sequence ID" value="CAZ86067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841876.1">
        <property type="nucleotide sequence ID" value="XM_002841830.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNH4"/>
    <dbReference type="FunCoup" id="D5GNH4">
        <property type="interactions" value="450"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GNH4"/>
    <dbReference type="GeneID" id="9186143"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011292001"/>
    <dbReference type="eggNOG" id="ENOG502QQ1T">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046464_0_2_1"/>
    <dbReference type="InParanoid" id="D5GNH4"/>
    <dbReference type="OMA" id="NRWLGKG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036286">
        <property type="term" value="C:eisosome filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070941">
        <property type="term" value="P:eisosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000005">
        <property type="entry name" value="Sphingolipid long chain base-responsive pil1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028245">
        <property type="entry name" value="PIL1/LSP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31962">
        <property type="entry name" value="SPHINGOLIPID LONG CHAIN BASE-RESPONSIVE PROTEIN PIL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31962:SF1">
        <property type="entry name" value="SPHINGOLIPID LONG CHAIN BASE-RESPONSIVE PROTEIN PIL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13805">
        <property type="entry name" value="Pil1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="270"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="167"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="278"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="333"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="357"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="388"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ86067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="403" mass="45657" checksum="F404E6016EF9239B" modified="2010-06-15" version="1">MHRTYSMRQSRAPTQSQIEKPPPPPSSTKVGRLFGGGNFGHNLRKSMAGAYGPELAKKLSQLVKMEKNLMRSMELVGRERRDVARQLSIWGEECDEDVSDITDKLGVLIFEIGELEDQFIDRYDQYRVTIKSIRNIEASVQPSRDRKQKITDQIAQLKYKDPNSPKIVVLEQELVRAEAESLVAEAQLSNITREKIKSAFTYQFDALREHSEKLALIAGYGKHLLELIDDTAVTPGETREAYDGYEASKSIIQDCEDALTSWVESKAAVKPRLSVRGSRQRRTHGRGTRSGNIGEPVSLTDQDRPMRGSGVWVPADEHERHRTHGAPALNRADDDDQYEDITDDEVDRGHHRGSRRHERDHEHDHGADRRQSPRNRDGKNADGLNGDAHSRDREDERGAVPQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNI4</accession>
    <name>D5GNI4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00011303001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430365">
        <property type="protein sequence ID" value="CAZ86077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841886.1">
        <property type="nucleotide sequence ID" value="XM_002841840.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNI4"/>
    <dbReference type="STRING" id="656061.D5GNI4"/>
    <dbReference type="GeneID" id="9186153"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011303001"/>
    <dbReference type="eggNOG" id="ENOG502SA8A">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_752695_0_0_1"/>
    <dbReference type="InParanoid" id="D5GNI4"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14688">
        <property type="entry name" value="bZIP_YAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42070">
        <property type="entry name" value="FILAMENT ASSOCIATED PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_8G06630)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42070:SF1">
        <property type="entry name" value="FILAMENT ASSOCIATED PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_8G06630)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="273"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="207"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="251"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="282"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ86077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="368" mass="39819" checksum="DB2628B975A6A320" modified="2010-06-15" version="1">MSNTIHTPSPSETSPPYATATGTKDNASRLRENQRRSRARKKEYLASLEARFQACQRAGVEANVEIQNAAKKVVRENLLLREMLRSRGVRDDEINRVIREGGEGSANGESAVEGVMKVLGRRPCGGEPLPVAKLGDERFNDRRNSTGMLAARDAGDRVSRCSETSCPPATGSGTCASPKCSSQLPLSPPSPVLQLPIHRPPPPPPSSAFTPQHYPSPSGTPTSAVPTYALPVSTPQLNPYYTFSSPPPPTSQSYTATSPTYPPANAAATGYQQFYPAPPQNASPQSSSNCCYPQQQQQQQQQQQQQQQQYQNPCTSSTTPCHLAQTFIHQLHPQDGGQLVNEICPPEAMGSGNCHVENSVLFDLLDKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNJ2</accession>
    <name>D5GNJ2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00011312001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430365">
        <property type="protein sequence ID" value="CAZ86085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841894.1">
        <property type="nucleotide sequence ID" value="XM_002841848.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNJ2"/>
    <dbReference type="FunCoup" id="D5GNJ2">
        <property type="interactions" value="463"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GNJ2"/>
    <dbReference type="GeneID" id="9186161"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011312001"/>
    <dbReference type="eggNOG" id="KOG1489">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011747_2_6_1"/>
    <dbReference type="InParanoid" id="D5GNJ2"/>
    <dbReference type="OMA" id="VGEWAFT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01898">
        <property type="entry name" value="Obg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.210.12:FF:000001">
        <property type="entry name" value="GTPase Obg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.210.12">
        <property type="entry name" value="GTP1/OBG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014100">
        <property type="entry name" value="GTP-bd_Obg/CgtA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006169">
        <property type="entry name" value="GTP1_OBG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036726">
        <property type="entry name" value="GTP1_OBG_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045086">
        <property type="entry name" value="OBG_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11702">
        <property type="entry name" value="DEVELOPMENTALLY REGULATED GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11702:SF31">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOME-ASSOCIATED GTPASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01018">
        <property type="entry name" value="GTP1_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002401">
        <property type="entry name" value="GTP_bd_Obg/CgtA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82051">
        <property type="entry name" value="Obg GTP-binding protein N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51883">
        <property type="entry name" value="OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Obg" evidence="5">
        <location>
            <begin position="95"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="OBG-type G" evidence="4">
        <location>
            <begin position="263"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ86085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="533" mass="59030" checksum="4DE4692F59442E61" modified="2010-06-15" version="1">MPLRLVQGLPQSSRIWITQLNTWTPFLYPFLAPGIAARPLTPTLSRPHRRALSTTTEQPELRSNVNSVQHQLVEEDVELVSVQQPQHPYAEYSTAPFADMQELTLIAGSGGNGCVSFLREKFIPYGPANGGNGGSGGSIFVQAVYGETSLHKLSREGTIRAKPGRNGKGSSINGKRGDDVIIRVPVGTTHHLSASTMLEITHPRNVYLDLSKPTMEPILLVPGAPGGLGNPNFVTDGRRRPMFATKGGKGIRMKIQLELKMLADIGLVGLPNAGKSSFLRAVSGRKARVGEWAFTTLAPNIGTVVMEERHLPPPRPGETQKRLPRFTIADIPGLIADAHLNRGLGHGFLRHIERAKALCFVIDLSREDPVEDLKGLWREIKAYEQGFVGDEGESTVRGMMSEMSGETHSDDRFIEPMAEACEPERRRTPTTMHIRFNEEDGTFEYFEPESGFVFSQKPRNEPPRMTSKRWFVIANKADLPNTEGKFVALQRYLETEKKRRGDREDIGLLPISALKQEGVEAAVDWMKSILGFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNR5</accession>
    <name>D5GNR5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00011441001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430367">
        <property type="protein sequence ID" value="CAZ86162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841971.1">
        <property type="nucleotide sequence ID" value="XM_002841925.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNR5"/>
    <dbReference type="GeneID" id="9182797"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011441001"/>
    <dbReference type="eggNOG" id="ENOG502S7E3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103432_0_0_1"/>
    <dbReference type="InParanoid" id="D5GNR5"/>
    <dbReference type="OMA" id="AFYGYAM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39605">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39605:SF1">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ86162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="182" mass="19791" checksum="87B8BD8FCF75CDAD" modified="2010-06-15" version="1">MDVYYCFNYLSSAWMLLEALPLITTPTIIISMLSSEVREPTQLESYLSRSLGLALIAFATLNLLLTGSIPLVSSFAATATVKGTSTDLEDPTAPYAVPSLSVTLTYHIAVGFYCYQQWALGRSATYAIGMIAHGFLAAMGVWVMMFASSNGHIYRKTGADDKSSSFPMTNMDGKKEKLGKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPT7</accession>
    <name>D5GPT7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00012026001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430378">
        <property type="protein sequence ID" value="CAZ86530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842339.1">
        <property type="nucleotide sequence ID" value="XM_002842293.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPT7"/>
    <dbReference type="GeneID" id="9182274"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012026001"/>
    <dbReference type="HOGENOM" id="CLU_2639898_0_0_1"/>
    <dbReference type="InParanoid" id="D5GPT7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ86530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="77" mass="8685" checksum="6528497043C18ADE" modified="2010-06-15" version="1">MTFTRYSYLITRDAPLCVALAPKNPRNSIAPPVRAAKSKKRAIEHSHLKHSNFPRMYSYSRVLGTSTTLEHSRAMGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPU1</accession>
    <name>D5GPU1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00012031001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430378">
        <property type="protein sequence ID" value="CAZ86534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842343.1">
        <property type="nucleotide sequence ID" value="XM_002842297.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPU1"/>
    <dbReference type="STRING" id="656061.D5GPU1"/>
    <dbReference type="GeneID" id="9182278"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012031001"/>
    <dbReference type="eggNOG" id="KOG1674">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018149_3_2_1"/>
    <dbReference type="InParanoid" id="D5GPU1"/>
    <dbReference type="OMA" id="VIRCEAH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20557">
        <property type="entry name" value="CYCLIN_ScPCL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013922">
        <property type="entry name" value="Cyclin_PHO80-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615:SF10">
        <property type="entry name" value="PHO85 CYCLIN-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin N-terminal" evidence="2">
        <location>
            <begin position="66"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="245"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="358"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="247"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="366"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ86534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="381" mass="42282" checksum="EDBCFE8CAFFC859E" modified="2010-06-15" version="1">MSRKDALEEFIRLPVSKEMISYLAVKAANVIKCESNAALPPTPPSTPPNGGVEPDFSALKPTLPTLEEFITSTVDRSRVQVPTLMSSLVYLQRLQKRLPAVAKGMRCTVHRIFLASLILAAKNLNDSSPKNKHWARYSVVKGFDGFGFSLTEVNLMEKQLLFLLDWDLRITTEDLYEHLDPFLQPIITQHEKEMEEKLEKEREFERHQSLMELRMEINSKSRPLLTSSMSSSSAYYLPVVTYGGDTPPSQHSTPPSSSGRSSRASERSVDSYYPRSSRSQRRSITLSDYSYPTPPSTAPSSTIIPGLVRSGTASSIASSEASSPNTPSVVMMRNLLPVDSCKKKSGTTASILTRFLHGGSSHEKSVSMSRSSSSRSYQQHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPW6</accession>
    <name>D5GPW6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00012080001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430379">
        <property type="protein sequence ID" value="CAZ86568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842377.1">
        <property type="nucleotide sequence ID" value="XM_002842331.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPW6"/>
    <dbReference type="GeneID" id="9182311"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012080001"/>
    <dbReference type="eggNOG" id="KOG3178">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005533_1_4_1"/>
    <dbReference type="InParanoid" id="D5GPW6"/>
    <dbReference type="OMA" id="DYFYTED"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016461">
        <property type="entry name" value="COMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001077">
        <property type="entry name" value="COMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012967">
        <property type="entry name" value="COMT_dimerisation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43712:SF5">
        <property type="entry name" value="O-METHYLTRANSFERASE ASQN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43712">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_4G14580)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08100">
        <property type="entry name" value="Dimerisation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00891">
        <property type="entry name" value="Methyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51683">
        <property type="entry name" value="SAM_OMT_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="O-methyltransferase dimerisation" evidence="5">
        <location>
            <begin position="74"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="O-methyltransferase C-terminal" evidence="4">
        <location>
            <begin position="188"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ86568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="418" mass="45729" checksum="33827C467464A020" modified="2010-06-15" version="1">MATRTEDLTELSNIISTSIKNYLSHVPTPPTLRPVPPIPVTDEVAVEVADELLVACRRVISLVEGPIRTALMMSSGFQDSIAMKLAYDMKLAQNVPPDGSPIALAELASKTGVPEETIVRVMRQLTSKNVFVETAPGYIAHTGGSAAIGLPGVGSMVAHLVDEGLTSAPHVSDILRENEFVVPEDSRKSAFSRAFNTNKSYFEYICTDNKPLGTRFGQAMKAMNVVDGLPNLVALYEPLQSAPKGTILVDIGGGLGHAAIAAAQKFPNLKCIVQDIGMVVSEGRDELPEELKDRVEFHENDFFEGQPIGGPGVYYYLKYILHDHPDPASKKILSHIVRAMDSSSRLLIDEHIVEERMGPDSHKLIPFLDFHMLLLFNSKERTEAQWAALLKGVDERLVVERVWRAKGTGNGIIEVRLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5G412</accession>
    <name>D5G412_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Sphingolipid delta(4)-desaturase</fullName>
            <ecNumber evidence="8">1.14.19.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00003900001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Delta(4)-fatty-acid desaturase which introduces a double bond at the 4-position in the long-chain base (LCB) of ceramides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>an N-acylsphinganine + 2 Fe(II)-[cytochrome b5] + O2 + 2 H(+) = an N-acylsphing-4-enine + 2 Fe(III)-[cytochrome b5] + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:46544"/>
            <dbReference type="Rhea" id="RHEA-COMP:10438"/>
            <dbReference type="Rhea" id="RHEA-COMP:10439"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:31488"/>
            <dbReference type="ChEBI" id="CHEBI:52639"/>
            <dbReference type="EC" id="1.14.19.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the fatty acid desaturase type 1 family. DEGS subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.14.19.17" evidence="8"/>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835134.1">
        <property type="nucleotide sequence ID" value="XM_002835088.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G412"/>
    <dbReference type="STRING" id="656061.D5G412"/>
    <dbReference type="GeneID" id="9182382"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003900001"/>
    <dbReference type="eggNOG" id="KOG2987">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032156_0_0_1"/>
    <dbReference type="InParanoid" id="D5G412"/>
    <dbReference type="OMA" id="GATCNQN"/>
    <dbReference type="UniPathway" id="UPA00222"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042284">
        <property type="term" value="F:sphingolipid delta-4 desaturase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046512">
        <property type="term" value="P:sphingosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03508">
        <property type="entry name" value="Delta4-sphingolipid-FADS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011388">
        <property type="entry name" value="DES1/DES2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013866">
        <property type="entry name" value="Sphingolipid_d4-desaturase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12879">
        <property type="entry name" value="SPHINGOLIPID DELTA 4 DESATURASE/C-4 HYDROXYLASE PROTEIN DES2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12879:SF8">
        <property type="entry name" value="SPHINGOLIPID DELTA(4)-DESATURASE DES1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08557">
        <property type="entry name" value="Lipid_DES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017228">
        <property type="entry name" value="Sphnglp_dlt4_des"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01269">
        <property type="entry name" value="Lipid_DES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="154"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="Sphingolipid delta4-desaturase N-terminal" evidence="10">
        <location>
            <begin position="9"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR017228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ79255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="367" mass="41958" checksum="0CFE0979AB2391E0" modified="2010-06-15" version="1">MAPAKASKQAAHDWLWTYQEEPHRTRRHQIIKAHPEVTKLCGPEPLTKYVVLFVVSLQIFCAFILQSTPVFSCQFFLMAYIIGATANQNLFLAIHEISHNLAFRSAFANRMIAIFANLPIGLPYSAAFRPYHLTHHKALGVDGLDTDLPTKIEAIFLDSVLGKAFFCTFQILFYALRPMMVYQLPFTKLHFFNVTAQLIFDAILVKACGWNALAYLIMSSFLAGSLHPCAGHFIAEHYVFERPKASPKDAISDGPAPVETYSYYGPLNFFTYNVGLHNEHHDFPAIPWTRLWTLREVAKEFYEPLPRHNSWSYVIWQFIWDSDVSLWSRVKREQQVGSGRRVTAGWTKEEIEDINGAVKNRGLEVVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4E2</accession>
    <name>D5G4E2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00004068001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835264.1">
        <property type="nucleotide sequence ID" value="XM_002835218.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4E2"/>
    <dbReference type="FunCoup" id="D5G4E2">
        <property type="interactions" value="1491"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G4E2"/>
    <dbReference type="GeneID" id="9185010"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004068001"/>
    <dbReference type="eggNOG" id="KOG2294">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007090_1_0_1"/>
    <dbReference type="InParanoid" id="D5G4E2"/>
    <dbReference type="OMA" id="EKYAFYR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00059">
        <property type="entry name" value="FH_FOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22701">
        <property type="entry name" value="FHA_FKH1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000030">
        <property type="entry name" value="Forkhead box protein K2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018122">
        <property type="entry name" value="TF_fork_head_CS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030456">
        <property type="entry name" value="TF_fork_head_CS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45881">
        <property type="entry name" value="CHECKPOINT SUPPRESSOR 1-LIKE, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45881:SF1">
        <property type="entry name" value="FORK HEAD PROTEIN HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00657">
        <property type="entry name" value="FORK_HEAD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="FHA" evidence="8">
        <location>
            <begin position="103"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="Fork-head" evidence="9">
        <location>
            <begin position="304"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="6">
        <location>
            <begin position="304"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="228"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="395"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="508"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="549"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="228"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="244"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="399"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="574"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="606"/>
            <end position="615"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ79385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="624" mass="68307" checksum="619DB90DD2832516" modified="2010-06-15" version="1">MPTSAIRRTRNRRDSTPDQHAQNSSPSRPRSKRRKVQATETSPDPPLDQINAVIASLAMPDPNVVVAVKYANDENYRQSAQGITAYAKVAGASWTYYVKELTIRIGRPPDARPGTAGSPTPPPQQKAEDIVHIDLGPSKLVSRSHAIITYDMAGDRNWQLRVLGRNGLKVNEEPYKKDAVIVLQSGSIIEIGGVQMMFVLPDRPASIHPSFLQRARVLAPIEDDLQLQTTQSNDSSNYDTATMPSSQGPRPSSSQQQLLPPAVITDNPSQPEPARRKDEPTFPRGVVLTAENIDYSDDSLKDMKPPYSYAMMIAQAILSSEGEQLTLSAIYSFITEKYAFYRHSNTGWQNSIRHNLSLNKAFRKIPRRTDEPGKGMKWELLAEHRDEYIKKIQKVTKTGSGRPGSAPGSPSSPKPPPSQALRLDDPHDLGTGYPTIKNSRSVTPPPVASYNRVAPLEAYTPDRGSRLPALRAQDNALNDLSTNPHHPRRSSIITATHTNDMISAVTPAPQRQHPRLAPPSTGQLPSSYLPTSSPAPFWKYVQFGSTPAKPGDYSPLKDLHSSSPPPVSGPEGSSRIRELGSPLKERGGGFHLAGSRGGTSLIPDIKEDDDEDELGDMQGVDLAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4L7</accession>
    <name>D5G4L7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00000002001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429989">
        <property type="protein sequence ID" value="CAZ79460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835303.1">
        <property type="nucleotide sequence ID" value="XM_002835257.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4L7"/>
    <dbReference type="STRING" id="656061.D5G4L7"/>
    <dbReference type="GeneID" id="9188419"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000002001"/>
    <dbReference type="eggNOG" id="KOG1176">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_5_1"/>
    <dbReference type="InParanoid" id="D5G4L7"/>
    <dbReference type="OMA" id="CGAPIVY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43859">
        <property type="entry name" value="ACYL-ACTIVATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43859:SF4">
        <property type="entry name" value="BUTANOATE--COA LIGASE AAE1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="5">
        <location>
            <begin position="34"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="6">
        <location>
            <begin position="475"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ79460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="567" mass="62450" checksum="08A6617338045FD8" modified="2010-06-15" version="1">MSSPQNRLKKIAGHFSPDRPVNRAELSPTYFLPRAAAIEPNVEAIVHLTANGKTVGRTYQQFADRARGLGYYLRAKGYKRVGILSPNTPAFLESIFGIGGAGAVQVAINYRLKRDDIHYIFDHAEVDSIIVDREYVHLLNGFKPEIPKIVDEDSGNDLEGDFNKAVLEGLEIDRRSGGRGWEGLAMEAGSEDDMIALAYTSGTTARPKGVEYTHRGAYLGALGNVVESGLNGGSVMPTGRAKYLTILPLFHACGWTFPWAVVSARATHYCLRKIDYHDMWRLLKDEGITHFNAAPTVNTLLCASPKAVRLPHEVRVTVAASPPTAKLFEDMINLNLVPVHVYGLVRLYGPITKGYFLPEWHSLPAADMYKMMARQGHGFITSKMIRVIKTTNDISKTGVQDVARNGQEIGEVAFTGNICCRGYYKDPEATAKLFEGGVLHSGDLAVWHPDGAIQILDRSKDIIISGGENISSLSLESMLVHHPDILECGVVAVPDAQYGERPMAFVTSRNPELKGTDVITWAKKDSQISGFMVPREVEIVKELPKTETGKVKKNVLREWSKGNRSAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4U0</accession>
    <name>D5G4U0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00000197001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429991">
        <property type="protein sequence ID" value="CAZ79533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835376.1">
        <property type="nucleotide sequence ID" value="XM_002835330.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4U0"/>
    <dbReference type="GeneID" id="9183015"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000197001"/>
    <dbReference type="HOGENOM" id="CLU_769852_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4U0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="202"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="293"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="325"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="237"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="296"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="360" mass="40004" checksum="35E875796569E9DB" modified="2010-06-15" version="1">MQRSPPPNVIVSTAATATTAVAQRSRHHRKNARVAFDDYFDEEPIPEDPEPEHEPEPEPKEEEEEEEEEEEEVGQEQKLCKREKARSKALAKLSGEYRREEDSDSETQSVFDFKAVFRNLVAPRSASPSSFLQDGDTPEGNSPPLAVRPYSGYDQRIIVPGDITGSWVLEERNRDRTDDTFDSRDSQDQKIANLKRELKAVANSDKRINSSSTNPLPLTNNQSSWINNSDTNTEHLTSISPTTSSPITAPSSSPRRYPPSGEGAYQNAHPYPPSCASPPLPDIEASMANHPLSAFDTHSSSQTTTGVRKKHRAKKFLWGGKKTQEEAQVEEVVGDGSEEKKGWKNSLKRSAIAKFLMGGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>D5G525</accession>
    <name>D5G525_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00000333001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429995">
        <property type="protein sequence ID" value="CAZ79618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835461.1">
        <property type="nucleotide sequence ID" value="XM_002835415.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G525"/>
    <dbReference type="GeneID" id="9186316"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000333001"/>
    <dbReference type="HOGENOM" id="CLU_1016329_0_0_1"/>
    <dbReference type="InParanoid" id="D5G525"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="150"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="83"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="201"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="88"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ79618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="274" mass="28621" checksum="29BE89F79030F188" modified="2010-06-15" version="1">MSSPPYSTSQLLTTVMKNPTVLVPTPSTTSTWESGDFRNITYSTIKLPAGATEDTTFQVTGPGGPTGLRRQGQEHLYTVIAIGQPLPSNESTSTTTTGNSASQPRSKPTAEPAPSPPAAPTDIPVATGGATLPTPLPAQQTGSSDNAPKVVAGVIGGLFLLTLAALLFLIFKLRKAAKDRALSPPAMVFTETSSAKGQSTISELSARNTDLMNQLEERNAQLSALQATLMRSNRIERLGHYTFQVHGTAPRSFTGAARDPDFSGSAIRSVAAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>D5G540</accession>
    <name>D5G540_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00000241001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429996">
        <property type="protein sequence ID" value="CAZ79625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835468.1">
        <property type="nucleotide sequence ID" value="XM_002835422.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5G540">
        <property type="interactions" value="84"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G540"/>
    <dbReference type="GeneID" id="9186323"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000241001"/>
    <dbReference type="eggNOG" id="ENOG502QQ5B">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002415_0_0_1"/>
    <dbReference type="InParanoid" id="D5G540"/>
    <dbReference type="OMA" id="DRSNCKR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070762">
        <property type="term" value="C:nuclear pore transmembrane ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006999">
        <property type="term" value="P:nuclear pore organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056541">
        <property type="entry name" value="Ig-like_POM152"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056542">
        <property type="entry name" value="Ig-like_POM152_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056543">
        <property type="entry name" value="Ig-like_POM152_9th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056544">
        <property type="entry name" value="Ig_POM152"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037701">
        <property type="entry name" value="Pom152"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056540">
        <property type="entry name" value="TMD_POM152"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28206">
        <property type="entry name" value="NUCLEOPORIN POM152"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28206:SF1">
        <property type="entry name" value="NUCLEOPORIN POM152"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24312">
        <property type="entry name" value="Ig-like_POM152"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24519">
        <property type="entry name" value="Ig-like_Pom152_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24527">
        <property type="entry name" value="Ig-like_Pom152_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23664">
        <property type="entry name" value="Ig_Pom152"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24097">
        <property type="entry name" value="TMD_POM152"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleoporin POM152 N-terminal transmembrane" evidence="3">
        <location>
            <begin position="82"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin POM152 first Ig-like" evidence="5">
        <location>
            <begin position="222"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin POM152 Ig-like" evidence="4">
        <location>
            <begin position="474"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin POM152 immunoglobulin-like" evidence="2">
        <location>
            <begin position="580"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin POM152 Ig-like" evidence="4">
        <location>
            <begin position="782"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin POM152 immunoglobulin-like" evidence="2">
        <location>
            <begin position="897"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin POM152 ninth Ig-like" evidence="6">
        <location>
            <begin position="1102"/>
            <end position="1180"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin POM152 Ig-like" evidence="4">
        <location>
            <begin position="1199"/>
            <end position="1269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF24519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF24527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ79625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1281" mass="140958" checksum="06B024BC8C3464EF" modified="2010-06-15" version="1">MSDGTPRIPAAFPRSPATGRARRVPHSPSPPARESPPRSVGAIVSTSMTSTIATGPKNNAPPSMVQTRDVALPPLISLDTLDAPTQRLYAVGFFVLLQAWKFYDIARLYTTDGDSISELWFCSKWLVVDGCFFWFLPLLRIPWLTFTPNFTLRTIAIFSVVDIFLSLKYEIPLTAIIGGLWKFIYDREVSISEKSVKWYDIVNNNSHIQGKYTVHILPEGAVKLNPNANCFCLGEGVSAINLPIRINGTNPIHIQLARIDFETGGADVIDISAKEIKKMLKRAEKGEDPKLQVLSYSVKQPGLYRLTKVTDVTELDVRIYRSEALVVSCPKAALKVSPTYKRNKCKGDMSDLALQVEGLAPLQITYSREMKGKPTVFTVQSIHPENFESPLLAGFSSDGTLAEQGDAALSWAQRQNIPVPLNESLGSSGDWVYSVDKVSDACGNAVDYNAAYEDGDYVVPKRGSLSQNLKVHDRPLARFLGCDPQHPMDLPKGATGVLPIQLLSGQDDAPYEVELTFTPYEKLGLASEHALGAAAGVHKLKTLSHVVSIKEPGLYSVKAISSDFCSGDVMEPASCLVITPPEPSMAMRSDEILDKCTGSSIGLTIDLTLVGAPPFELSYRLIKDNGNPAVKTLTIERTRHQVQFTPADAGHYAYEFFSLRDKNYANVKIDPKTNYVEQTVKPMAGATFVDPSPRKKVCIDQPVEFDVKMQGMPPLTLNYELIHNGKRTKMTEPNITTGIHTIRTPPLANGGEYALALTTVEDESGCQIFVGSEAKIDVRWQRPKAQFSSIEGKMSVRALERKTVDIPLRLTGESPWDVSIRNLDSNEPAKQLKFAGSNAYLTVRDPGRYKLESVHDNGCPGTITESDSTFLITWIARPMLKLAESSSTTKGDVFTRKDVCEGDEDAIELAFVGSPPFTIDYERTFRPEGYDSRRALDPIPQKLTAGLGVATVRLETSQAGVNRYKFSRIADSLYDSPRESLVQNPIILEQTIHSRPSTSFANPSKVYKYCLDAGAGDDIIPIQLHGKAPFSLTINIKHFNTGKSDVINIPHIDSNIFNFRIPSHALTLGSHAVSVLKVKDSRGCVRKTMEGPHVMVAVADMPTISPSSQQTNYCVGDRISFTLSGVPPFAVEYEWNGVLMKAHNQPSEFARVAEKPGNFTITGLQDSASDCKVNVGLTQIVREVPSVRISDGYTVVRGIPEGDQAEIHFDFYGTPPFTFTYTRSEIAKKGSKPRILEMHSQTTDGYKYSMFASQEGTYEVVSIEDSYCSYAAGKANSGRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>D5G542</accession>
    <name>D5G542_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ubiquitin-conjugating enzyme E2-16 kDa</fullName>
            <ecNumber evidence="1">2.3.2.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">E2 ubiquitin-conjugating enzyme 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Ubiquitin carrier protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Ubiquitin-protein ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00000245001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
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            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="11">Belongs to the ubiquitin-conjugating enzyme family.</text>
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        <property type="interactions" value="909"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="HOGENOM" id="CLU_030988_13_3_1"/>
    <dbReference type="InParanoid" id="D5G542"/>
    <dbReference type="OMA" id="VHFTTRI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="P:positive regulation of mitotic metaphase/anaphase transition"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="P:SREBP signaling pathway"/>
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        <property type="entry name" value="Ubiquitin-conjugating enzyme E2-16 kDa"/>
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        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="UBC_2"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 11" id="KW-0833">Ubl conjugation pathway</keyword>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="10">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00030012"/>
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    </evidence>
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        <source>
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    </evidence>
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        </source>
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        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAZ79627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="147" mass="16458" checksum="9448B6A2A5489F2F" modified="2010-06-15" version="1">MALKRINKELSDLGRDPPSSCSAGPIGEDLFHWQATIMGPADSPYSSGVFFLSIAFPTDYPFKPPKVNFTTRIYHPNINANGSICLDILRDQWSPALTISKVLLSICSMLTDPNPDDPLVPEIAHVYKTDRARYEATAREWTRKYAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5G568</accession>
    <name>D5G568_TUBMM</name>
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        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00000279001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN429996">
        <property type="protein sequence ID" value="CAZ79653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_002835450.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="9186351"/>
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    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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    <dbReference type="InParanoid" id="D5G568"/>
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    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="VerF-like"/>
    </dbReference>
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        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
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        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
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        <property type="entry name" value="MIP31509P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40626:SF32">
        <property type="entry name" value="ZINC FINGER PROTEIN RST2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="109"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="140"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="159"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="338"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="374"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="514"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="556"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="641"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="30"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="49"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="63"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="282"/>
            <end position="296"/>
        </location>
    </feature>
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            <begin position="307"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="338"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="374"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="428"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="601"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="663"/>
            <end position="677"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ79653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="732" mass="78482" checksum="1E9E017D85F55B9E" modified="2010-06-15" version="1">MPSDDQLGPPSFQAVNKMSSRSKGSLGVNGVSSSRPPTSSIVFLPNSPAPVQNVPNTGFQLPSNTSAATSSENAQAESGRSSPRSSEGRSDGEAPRSPTKNKKKKGQKFFCKGYGTCNLSFTRSEHLARHIRKHTGERPFNCHCGRAFSRLDNLRQHSSTVHADEPIPDDSLAAVSSRYQRHQRTERTRQTARTANRSKPRSNSGPKPPTSGPGLSGHSQPQTPPQPSQPLGATQAPGAFTDTPAKYQSGLQDSWGGVTGPRNYGGVSQVPQTPHNPSPHPVEMHQRHEARQRNRPEPIMLPDLTSPFENYRNNTPPESPASVLGSRENSFYQGRRASFTQPLASNMTTPTSATFTGANESPIGSPIFPSLNSASSSVINSPASAFPSTSSWGSGTRHPGRRLSVPGSGLHSDRMLPMPGPALRYRSTAAPAPAPGTAASLRARSGMAMSPEEEEEKIKRRRTWHESGPEASAARELFSPSNTSHGDRSLLGIGGQSASQSHTQLPAISSLLNLNDTSPSLRHTPPPRRAPVEPEPTHDLSIRMASLGGGLRTMSSRTLRGHSRSQSDDFMSGNSRHERRWGMHSSTDAPSVRWSGDTRDNISQSWNSPGSRREPNGHLPGILSSPRHPEPLRQAFVYPSSGSGVQSGWGRKRCHRDSVGSGEDTSGSEGVVTPSTNEELHPGIVGEGTAEVDEPHIVSPSLFFSFPPTPLDLCSDEGLEPPQADAYCSGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5F8</accession>
    <name>D5G5F8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00004315001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835594.1">
        <property type="nucleotide sequence ID" value="XM_002835548.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5F8"/>
    <dbReference type="GeneID" id="9183737"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004315001"/>
    <dbReference type="HOGENOM" id="CLU_1031310_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5F8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003071986" evidence="2">
        <location>
            <begin position="21"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="200"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ79751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="270" mass="28588" checksum="20393014DE3E212D" modified="2010-06-15" version="1">MRLLAVSAVLFLNAALAVQAAPLAHAHHPRGFELVARNGGRGGDNNRREEEKRRYDERKKQQEEEEKQQEEELQRLLEQLQQEQEKKEEDERKKVQDELKKAEELRRQNRDRYENKDVVVVVVQIIVIVDSQGNPSPPQPHTVAKVLYKQEGAVATETQYATEPGTLTLVQTEAAANATEAAGATATEITVTDATATETAAETAADGAEATATDTAGVEATGSAPTAGPTATATATAPPTAPTGNPTAPVTTTSAATAPPGGTPTAPVAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5H3</accession>
    <name>D5G5H3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00004335001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835609.1">
        <property type="nucleotide sequence ID" value="XM_002835563.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5H3"/>
    <dbReference type="GeneID" id="9183752"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004335001"/>
    <dbReference type="HOGENOM" id="CLU_3320328_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5H3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ79766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="39" mass="4094" checksum="CB7DBBF90128E103" modified="2010-06-15" version="1">MEVEGGGNDWGGVVADRARLEVRTGGSEPCLGFLGDMRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5V1</accession>
    <name>D5G5V1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pre-mRNA-splicing factor SLU7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00001588001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in pre-mRNA splicing.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Associated with the spliceosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the SLU7 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430002">
        <property type="protein sequence ID" value="CAZ79894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835737.1">
        <property type="nucleotide sequence ID" value="XM_002835691.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5V1"/>
    <dbReference type="FunCoup" id="D5G5V1">
        <property type="interactions" value="473"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G5V1"/>
    <dbReference type="GeneID" id="9187572"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001588001"/>
    <dbReference type="eggNOG" id="KOG2560">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019317_3_1_1"/>
    <dbReference type="InParanoid" id="D5G5V1"/>
    <dbReference type="OMA" id="KYAWESQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030628">
        <property type="term" value="F:pre-mRNA 3'-splice site binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021715">
        <property type="entry name" value="Slu7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039974">
        <property type="entry name" value="Splicing_factor_SLU7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12942:SF2">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR SLU7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12942">
        <property type="entry name" value="STEP II SPLICING FACTOR SLU7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11708">
        <property type="entry name" value="Slu7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5 7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Pre-mRNA-splicing factor SLU7" evidence="9">
        <location>
            <begin position="114"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="143"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="255"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="405"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="143"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="272"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="417"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF11708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ79894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="472" mass="54765" checksum="DD65BCB7D7B81E91" modified="2010-06-15" version="1">MSSSKRNPEKQAKNEYIPHFIAKKPFYLDHDTEGDYLEHQRLQNKKDLDLANQKWYDRGKRAGPAATKFRKGACENCGAMTHKAKDCLSRPRKKGARWTGQDIQADEIIQEVELGWDGKRDRWNGYDSHEHKKVVEEHMRMEELRRESERKSAEDVEMEGGGDEDKYAEESEMPGQTYDAASRISTRNLRIREDTAKYLLNLDLDSAKYDPKTRTMVDRGATQDQAAALVEEDNFMRSSGDAAEFERLQRAAWESRERGNKTKMHLQANPTEAEHIKKKEQQAAEEKRARERKALLERYGGEQHLVDKPPVLEEEERYIEYTASGQVKGREKTTAKSKYTEDKLINNHTTVWGSWWQSFKWGFACCHSTVKNSYCTGEEGIRAAEEANKLRLGLIEPVAPRWVEQKQEEQEEVAEEDSVKDKSKGKDGEDHVDRGKRKLKEMRDGVTEEDMEQYRKQQKLYSDPMANFVDTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5Z5</accession>
    <name>D5G5Z5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="12">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00001695001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCA family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430005">
        <property type="protein sequence ID" value="CAZ79938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835781.1">
        <property type="nucleotide sequence ID" value="XM_002835735.1"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G5Z5"/>
    <dbReference type="GeneID" id="9187616"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001695001"/>
    <dbReference type="eggNOG" id="KOG0059">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001640_2_0_1"/>
    <dbReference type="InParanoid" id="D5G5Z5"/>
    <dbReference type="OMA" id="WKNWIVL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005319">
        <property type="term" value="F:lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03263">
        <property type="entry name" value="ABC_subfamily_A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001345">
        <property type="entry name" value="Related to ABC transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026082">
        <property type="entry name" value="ABCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229:SF36">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY A MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER SUBFAMILY A ABCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12698">
        <property type="entry name" value="ABC2_membrane_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="221"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="271"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="306"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="334"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="359"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="402"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="798"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="976"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1018"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1047"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1082"/>
            <end position="1107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1119"/>
            <end position="1141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1162"/>
            <end position="1184"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="451"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="1233"/>
            <end position="1459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ79938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1575" mass="172982" checksum="ACF5203D8AABDEA2" modified="2010-06-15" version="1">MGNLGRQTWALWKKNVRIVLLRHPLATIFRSVVLPIFFIGFISYARNLFVPPATYGVGTPAHVKDLQRELLAHSDKKLAFVHNNLGSEVRDLIDGIASPLIAAGIQVAILEKNEDLRIECKQTLRGSSPCFSAAVFDDSPASSDNGVWNYTLRGDSSLDGNKINVKDHDNDVQTFLMPLQYAIDRAIAGRARQGSGDVVVDEYMYTSLSQKEREDKIRRDYMTGITQYIGVAVFLGHVGIIYHQTGFQALERERGISALLESMGVSKSARILAYFLSFSWVYSGGWVVMGLTMARGVFATTNAGIILVWFILTGLSCASFSIFASSFFKRTQLSGISTTLISIVLGIGAQVCHGSSSGAIAILSLICPSSNFVLMAVILGRFEKKGQAASLTKSPPDQASTIPAIVLFVFLIIQIIGFAIGAAYVEKWLYGTESPGHRSSNPALIAPENAVEIRAFTKRYKPSILGNLLAAGKKETVTAVNKLDLDIRQGQVLVLLGANGSGKTTTLEAIAGLATVDEGEIRINTGKKGGIGVCPQKNVFWDELTVEEHVGIWNKLKCEGDSKEILHQLLRDCDLLVKKKAKSSTLSGGQKRKLQLAIMFTGGSNVCAIDEVSSGLDPLSRRKIWDIILAARGERTIILTTHFLDEADLLADHIAILSKGTLKCEGSAVALKAKMGGGYRVHVPRYFPEVSGVSAKRLYDQTVYNIPDSTSAGELVDKLETRGVDEYHVSGPTIEDVFLKVAEETADIQDQQLRQSPSTEAAEKESEDVNLYAGERLGIFKQTWVMFRKRLVIFKRNYFPIVAAIAIPILAAGLTMIFLKDYDKASCSNADQTSTADVERLTFNTKFNVTLGPRSNFPAQVLETLASTILPADIGGTQNETLMRSFNDSLHFVDSLDDFERNIKINYHNVTPGAIYLGGRDPSTNATIAFKGNRNVRNALIAQNMVDNLLLGQRIVTRYAEFDFFWSDGQGLTLQFVVYMGLALSAFPAFFALYPTVERIRNVRALHYSNGVRALPLWLAYTAFDFVAVLIISVICTIIYVASTNDVWFHIGYLFLVLLLYGLASVLLAYNVSLMAKGQLSAFAIVAGVQAVMFLLYFIAFTSVLTYAPASEMDDQINIVHFAFSVPLPIGSLARALFLALNIFSISCRGEEWATNPGEITLYGGPILYLIMQSICYFLLLLWWDSGRFRLALPWRRVSTVNSDSEPDVGAAGAEKQHINDEITRVTSSNDGLRVVGITKSFSRKLVAVESVTFGVARGEVFALLGPNGAGKTTTINMIRGDMAPDSGEVFVENIPVTRRRAEARSHLGVCPQFDAMDRMTVAEHLRFYAQIRGVKDVEHNVSQVIRAVGLEAFQTRMGEKLSGGNKRKLSLGIALMGNPTVLLLDEPSSGMDAAAKRTMWKTLAGIQKGRSLVLTTHSMEEADALASRAGILAKKMLAVGTSDDLRKRWGDGYYIHLVLKSAPASTEAEMHNVKDWVLRRFRGAVVEQRSFHGQVRYSIPIWRGEDENESDITPTGASRRAGTGVNRALGLEYYSVSQTTLDQVFLTIAGSANVAEEGYEANKKKKWFRFWNKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5G659</accession>
    <name>D5G659_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00001783001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430007">
        <property type="protein sequence ID" value="CAZ80002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835845.1">
        <property type="nucleotide sequence ID" value="XM_002835799.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G659"/>
    <dbReference type="GeneID" id="9187681"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001783001"/>
    <dbReference type="HOGENOM" id="CLU_1950378_0_0_1"/>
    <dbReference type="InParanoid" id="D5G659"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ80002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="129" mass="14566" checksum="F62CA6985151938C" modified="2010-06-15" version="1">MVTISRGVNVDAEDSTHDSLLLEPPLTLPFLLLVILTAKSNDYHPSHKIGGELLLGSTPEHPFQPRYHTSLAYHKPAVVKYHCLNPKLKLIGDRSIIDISWPYVHGRDKRPHATCRLFEYALSSWSGRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6G4</accession>
    <name>D5G6G4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00004470001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430009">
        <property type="protein sequence ID" value="CAZ80107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002835904.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6G4"/>
    <dbReference type="GeneID" id="9181192"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004470001"/>
    <dbReference type="eggNOG" id="KOG4062">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_52_5_1"/>
    <dbReference type="InParanoid" id="D5G6G4"/>
    <dbReference type="OMA" id="DEAEWWF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032132">
        <property type="term" value="F:O6-alkylguanine-DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070911">
        <property type="term" value="P:global genome nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006283">
        <property type="term" value="P:transcription-coupled nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06445">
        <property type="entry name" value="ATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052520">
        <property type="entry name" value="ATL_DNA_repair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014048">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036217">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNAb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42942">
        <property type="entry name" value="6-O-METHYLGUANINE DNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42942:SF1">
        <property type="entry name" value="ALKYLTRANSFERASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01035">
        <property type="entry name" value="DNA_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46767">
        <property type="entry name" value="Methylated DNA-protein cysteine methyltransferase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methylated-DNA-[protein]-cysteine S-methyltransferase DNA binding" evidence="2">
        <location>
            <begin position="10"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ80107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="141" mass="15138" checksum="B56517C4B5912E44" modified="2010-06-15" version="1">MPHSDEATAFFVAVYEAVQEIPYGKVTSYGHIAHLIGKRKLEQVGQALKHLPADPANTFHHGNVPWQRVIAASGVIPRRDNPSGAARHAQALRGEGVVVTTTGGGLHVDLSGEHGWFPNVLPSKGGGGDWVNEETMFPVLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6L7</accession>
    <name>D5G6L7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00002101001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430011">
        <property type="protein sequence ID" value="CAZ80160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836003.1">
        <property type="nucleotide sequence ID" value="XM_002835957.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6L7"/>
    <dbReference type="STRING" id="656061.D5G6L7"/>
    <dbReference type="GeneID" id="9187186"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002101001"/>
    <dbReference type="eggNOG" id="ENOG502SE9Z">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_562825_0_0_1"/>
    <dbReference type="InParanoid" id="D5G6L7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.60.10">
        <property type="entry name" value="Cyanovirin-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011058">
        <property type="entry name" value="Cyanovirin-N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036673">
        <property type="entry name" value="Cyanovirin-N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42076:SF1">
        <property type="entry name" value="CYANOVIRIN-N DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42076">
        <property type="entry name" value="CYANOVIRIN-N HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08881">
        <property type="entry name" value="CVNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01111">
        <property type="entry name" value="CVNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58100">
        <property type="entry name" value="Bacterial hemolysins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51322">
        <property type="entry name" value="Cyanovirin-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyanovirin-N" evidence="2">
        <location>
            <begin position="4"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="323"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ80160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="485" mass="53490" checksum="4AC64B3F9E70EADC" modified="2010-06-15" version="1">MALSYHKSSVYISLSGNQVLSALCRTCDGEWVRSTIDLNTVLGNCDGRFEWGETRFSHTAKDVKLKDNRTLSAYLKRKDGSWSEKPVEVNLTEHITNHDGALRNCQRAAEQQDQEPSVAPNFLEKLDAVSGLLRSQQIAAKNSGFELEFVSASNNRLMLEELEVAFLRLRKLMGVISGEFDQEGEGGEDTDAGRRKRAEAEAAVRPMIKAFEKVRGKGDKSLAAVSRICGATAEYETAHLPIIRRDVESLRAEIDLQVKDVQVSLETLQEAVTTSTTELESTQDVLKRSKSTKGKANSIETLIKHAGWILLPIGIGMTIPLTATALADKITAAEEKLKELTTQQAEKQQELDNLKLQESESALALRACEDLTAQFTVLEADVLRLKTTAELARENITSTLTTAKTLESLAREMEDKASMLEYRVCKKDYAAHIVRVLDEALPGFAVVGDVGELLTELLAGDDSQGSVKELNLEIEVVRRKLQVVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6V7</accession>
    <name>D5G6V7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00002295001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430017">
        <property type="protein sequence ID" value="CAZ80250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836093.1">
        <property type="nucleotide sequence ID" value="XM_002836047.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6V7"/>
    <dbReference type="GeneID" id="9182474"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002295001"/>
    <dbReference type="HOGENOM" id="CLU_2051366_0_0_1"/>
    <dbReference type="InParanoid" id="D5G6V7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ80250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="120" mass="13522" checksum="A6197FE2F5B252AE" modified="2010-06-15" version="1">MGEREYGYLSTGMVAAESSLPRIYSRALAFPSSAALGKLQPTETTAPYHTGTVQVRVLECEQTKGICTQLQYHAQRLTRRGNYNVLAHLSSIRASTLQHRYANRRYPPFVEQSLDETKTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7N8</accession>
    <name>D5G7N8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00004656001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430031">
        <property type="protein sequence ID" value="CAZ80531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836340.1">
        <property type="nucleotide sequence ID" value="XM_002836294.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7N8"/>
    <dbReference type="STRING" id="656061.D5G7N8"/>
    <dbReference type="GeneID" id="9187010"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004656001"/>
    <dbReference type="eggNOG" id="KOG4354">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1897705_0_0_1"/>
    <dbReference type="InParanoid" id="D5G7N8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050085">
        <property type="entry name" value="AGPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058924">
        <property type="entry name" value="AGPR_dimerisation_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32338:SF10">
        <property type="entry name" value="N-ACETYL-GAMMA-GLUTAMYL-PHOSPHATE REDUCTASE, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32338">
        <property type="entry name" value="N-ACETYL-GAMMA-GLUTAMYL-PHOSPHATE REDUCTASE, CHLOROPLASTIC-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22698">
        <property type="entry name" value="Semialdhyde_dhC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyl-gamma-glutamyl-phosphate reductase dimerisation" evidence="1">
        <location>
            <begin position="2"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="134" mass="14576" checksum="1753610F1AC6A35D" modified="2010-06-15" version="1">MPYSLTDHIHEHEIEARLGVPVAFIPHVAVWFQGIHHTVSVPLAKEMTSREIRNLYQDRYAGEKLIKVTGEAPLVKNISGKQGVEVGGFGVHSSGKRVVVCATIDNLLKGAATQCLQNMNLALGYGEYDGIPLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7R4</accession>
    <name>D5G7R4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00004689001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430031">
        <property type="protein sequence ID" value="CAZ80557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836366.1">
        <property type="nucleotide sequence ID" value="XM_002836320.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7R4"/>
    <dbReference type="FunCoup" id="D5G7R4">
        <property type="interactions" value="565"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G7R4"/>
    <dbReference type="GeneID" id="9181376"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004689001"/>
    <dbReference type="eggNOG" id="KOG1906">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_533389_0_0_1"/>
    <dbReference type="InParanoid" id="D5G7R4"/>
    <dbReference type="OMA" id="RIQSEIF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031499">
        <property type="term" value="C:TRAMP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990817">
        <property type="term" value="F:poly(A) RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010605">
        <property type="term" value="P:negative regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043634">
        <property type="term" value="P:polyadenylation-dependent ncRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05402">
        <property type="entry name" value="NT_PAP_TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054708">
        <property type="entry name" value="MTPAP-like_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045862">
        <property type="entry name" value="Trf4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23092:SF15">
        <property type="entry name" value="INACTIVE NON-CANONICAL POLY(A) RNA POLYMERASE PROTEIN TRF4-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23092">
        <property type="entry name" value="POLY(A) RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22600">
        <property type="entry name" value="MTPAP-like_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81631">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Poly(A) RNA polymerase mitochondrial-like central palm" evidence="2">
        <location>
            <begin position="4"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="332"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="417"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="458"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="332"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="465"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="481"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ80557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="511" mass="58355" checksum="C3BF9D8168FBC378" modified="2010-06-15" version="1">MTDRLASFVTRLCLSSEVIAGRGASIVRQRCVVHSIWPRASCYPVGSFQTRTSLKNSDVDLLVSIPGQSDEHKCMVEAATRMREAIIGRRIAEPRNCTVIGGAKVPILTYTDEKVTPPLKFDISFQKENAVRNTAYLVQTFDERPFMRDLVILMKYWLRERRLNEVYKGGLGSYAVSLTVIGFFNVNRRTLIPRDYQALITGSRHDMFVEYLKFWTRWKYNEDILVPDPGIIQKMAPGKRKRLPFMLRMVDPTDPDNDVGKAAYKIPKVITAMKKLRHGMENLGPDTGVSELDKLLSGTLGEKERYLKEKFHQDRNDRKMFRQMQKRIEEVERKAVKSGREGKSVKKSLARMRASRDEFARRRPALAAEFARPEPLPEDTGAPVHDPPGPGYDFDLDTLMIGPVEKEVSRWIPKEPTEAKSQNDETLTPIMSGGKSPEQLQLERAKHIATIEAKRVRREKTLQKRHEKLLKKKEKKAQKKIAAEKKLLEGEEELAKRPKASKVAAEKEAGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8H8</accession>
    <name>D5G8H8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00002880001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430046">
        <property type="protein sequence ID" value="CAZ80825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836634.1">
        <property type="nucleotide sequence ID" value="XM_002836588.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8H8"/>
    <dbReference type="GeneID" id="9186045"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002880001"/>
    <dbReference type="HOGENOM" id="CLU_1435415_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8H8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="189" mass="20555" checksum="47C3E1CA3B1575B1" modified="2010-06-15" version="1">MCLRRIEVYVGAGMGSNSDRNGMTGRGNTPDWPTHRCGDREGKAGILRITNILNPLREVAPTSPPSITENQACSGGPVSVLWGNDSGNDEFDPIDLNALLQFVYILDDYHHQTASWNVSDAGCKYLTGILGKQSSDSSRRFSSFIDGFCFGPLLDHSTNCLLPNQSLEVRDRGGSVERECIQGFGDVPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8N6</accession>
    <name>D5G8N6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00003032001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="FN430048">
        <property type="protein sequence ID" value="CAZ80879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836688.1">
        <property type="nucleotide sequence ID" value="XM_002836642.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8N6"/>
    <dbReference type="STRING" id="656061.D5G8N6"/>
    <dbReference type="GeneID" id="9186247"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003032001"/>
    <dbReference type="eggNOG" id="ENOG502S8H7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022194_1_0_1"/>
    <dbReference type="InParanoid" id="D5G8N6"/>
    <dbReference type="OMA" id="RIMRVDQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10917">
        <property type="entry name" value="CE4_NodB_like_6s_7s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.370">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002509">
        <property type="entry name" value="NODB_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46471">
        <property type="entry name" value="CHITIN DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46471:SF6">
        <property type="entry name" value="GLYCOSYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51677">
        <property type="entry name" value="NODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-0170">Cobalt</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072242" evidence="7">
        <location>
            <begin position="21"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="NodB homology" evidence="8">
        <location>
            <begin position="201"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ80879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="404" mass="44886" checksum="B6F0409BBB13AC25" modified="2010-06-15" version="1">MNTRNLFLLALAGFTSGVLSENIVIDDFKKQGTNNLNVYHGCDEGGGISCSWGGDGLTIRSSDVDYSYYSQFTSQRGDGGCQDISSWERRFVHIKFSGSPDFSVALQQNNGQCSTTQAPYPETWDIVYAADYSDGSDIYIPISHFSIDRSRAIGFAFKAFRNAGAETKFSLLEIVDALPGGRSVPEKKPTGPLYFACTRPNSIAFGIDDGVPEFAQQVMDIIEQENIKVTFFTVGQALDDSSQNFKQVYQDAIKKGHQVALHSYTHPKIEGLLDLSVIDNEFRLSRESTLRNLGVDSKYFRAPYGTDGALTRQRLQANVPGAKVINWSVDIEDWRWGQSPSPENLKQTEAVTRDLGKGGNLFVAHYLYPSTVEQFRTFIQLAKKTGKTIMRIDQCLEDPEAPPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8W0</accession>
    <name>D5G8W0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00004863001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430053">
        <property type="protein sequence ID" value="CAZ80953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836762.1">
        <property type="nucleotide sequence ID" value="XM_002836716.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8W0"/>
    <dbReference type="FunCoup" id="D5G8W0">
        <property type="interactions" value="103"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G8W0"/>
    <dbReference type="GeneID" id="9181236"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004863001"/>
    <dbReference type="eggNOG" id="KOG0108">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028601_0_1_1"/>
    <dbReference type="InParanoid" id="D5G8W0"/>
    <dbReference type="OMA" id="NEYEIMG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12398">
        <property type="entry name" value="RRM_CSTF2_RNA15_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.70:FF:000001">
        <property type="entry name" value="Cleavage stimulation factor subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.70">
        <property type="entry name" value="Transcription termination and cleavage factor, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025742">
        <property type="entry name" value="CSTF2_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026896">
        <property type="entry name" value="CSTF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038192">
        <property type="entry name" value="CSTF_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45735">
        <property type="entry name" value="CLEAVAGE STIMULATION FACTOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45735:SF2">
        <property type="entry name" value="CLEAVAGE STIMULATION FACTOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14327">
        <property type="entry name" value="CSTF2_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14304">
        <property type="entry name" value="CSTF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="10"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="199"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="199"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="214"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="227"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ80953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="287" mass="30240" checksum="A897CA41B8BC734B" modified="2010-06-15" version="1">MPPDRSATGKIVFVGNIPYGLTEEQITEIFSSVGRVLSFRLVYDRETGRPKGFGFAEYSDQETAASAVRNLDNYEIMGRKLRVDFSHEGSATEGMGMDDIMTQASGSGVLPALPLGADLPAGLNAPDAISRTLSALQPPQLLDILGQMKALVTADPLKATELLRQAPQLSYAIFQALLLMNLVDTSVLTQVIENAAAPSQPLPASASTTSSAYVPPPAVPAAPTPPQQQQSPYTPAPAQAAIVADPQRAALIQQVLSLTPEQIGALPADQQSQIMLLKHQLAAGGGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8Y8</accession>
    <name>D5G8Y8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00004900001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430053">
        <property type="protein sequence ID" value="CAZ80981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836790.1">
        <property type="nucleotide sequence ID" value="XM_002836744.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8Y8"/>
    <dbReference type="GeneID" id="9181264"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004900001"/>
    <dbReference type="HOGENOM" id="CLU_2028413_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8Y8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="122" mass="12833" checksum="E6472DA72C46B830" modified="2010-06-15" version="1">MGRPQPKPKRGGKKKGAHTTLVKASLPSPAKPRGRPKKSTKKDLKVQNDGDESPQGQGKSAEGGGITAGEIGVIFEILTAGADWAAIVAETNALIGLKRLGQNVSKHWKTIAKKRLLDAYGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9C3</accession>
    <name>D5G9C3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00003240001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430059">
        <property type="protein sequence ID" value="CAZ81116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836925.1">
        <property type="nucleotide sequence ID" value="XM_002836879.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9C3"/>
    <dbReference type="FunCoup" id="D5G9C3">
        <property type="interactions" value="29"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G9C3"/>
    <dbReference type="GeneID" id="9182887"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003240001"/>
    <dbReference type="eggNOG" id="ENOG502R3S1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028899_0_0_1"/>
    <dbReference type="InParanoid" id="D5G9C3"/>
    <dbReference type="OMA" id="HKLLLFW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13384">
        <property type="entry name" value="G PATCH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13384:SF16">
        <property type="entry name" value="GROWTH REGULATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="295" mass="32546" checksum="B73D9523E54BC949" modified="2010-06-15" version="1">MAATTGSASIITQVQQGGPPLTTLGDSPSEENLTLDLRGTKFVLSRDELLTLPEFVLLSLFPNGLFPDGQVTSFPDEGVHSVDFDPVCLQYMLDFFRQVSSTITASNSGHDDADMENGASPSIPTNVLQDRAGIIVLREDLDFYVIPPRRDISHPDMASVKRNAGKLLLKQDGIFSGLRRTDDAGTTEQHLIEMLTAGGFNTDDRWGHRAGEPNKAVICSLALARLRTDMRSATGEGKDSSAVGMAQKLLLFWRKPARRCWWEGMDLENVEGVDGRLKVWIRRVWTLEMSVIGLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9Z6</accession>
    <name>D5G9Z6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00003469001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430068">
        <property type="protein sequence ID" value="CAZ81339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837148.1">
        <property type="nucleotide sequence ID" value="XM_002837102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9Z6"/>
    <dbReference type="GeneID" id="9187037"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003469001"/>
    <dbReference type="HOGENOM" id="CLU_2575585_0_0_1"/>
    <dbReference type="InParanoid" id="D5G9Z6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ81339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="81" mass="9198" checksum="C37BB8CA00B35D86" modified="2010-06-15" version="1">MAVLAKQIDRPLRHQLYRYGIIPHRIARTLKKKSDQPRYIQNSTSNSSLIPALDRVKPVPGCRTPNTVTTLGRQFAVIMLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5GA29</accession>
    <name>D5GA29_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 14">Uroporphyrinogen decarboxylase</fullName>
            <ecNumber evidence="4 14">4.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">GSTUM_00003545001</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the sequential decarboxylation of four acetate groups of uroporphyrinogen-III (octacarboxyporphyrin) to yield coproporphyrinogen-III (tetracarboxyporphyrin) with the formation of intermediate hepta-, hexa- and penta-carboxylate porphyrinogens in the heme biosynthesis pathway. Acts on a number of porphyrinogens, but only coproporphyrinogen III can ultimately be converted to heme.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>uroporphyrinogen I + 4 H(+) = coproporphyrinogen I + 4 CO2</text>
            <dbReference type="Rhea" id="RHEA:31239"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:62626"/>
            <dbReference type="ChEBI" id="CHEBI:62631"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 14">
            <text>uroporphyrinogen III + 4 H(+) = coproporphyrinogen III + 4 CO2</text>
            <dbReference type="Rhea" id="RHEA:19865"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57308"/>
            <dbReference type="ChEBI" id="CHEBI:57309"/>
            <dbReference type="EC" id="4.1.1.37"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 4/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the uroporphyrinogen decarboxylase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.37" evidence="4 14"/>
    <dbReference type="EMBL" id="FN430071">
        <property type="protein sequence ID" value="CAZ81372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837181.1">
        <property type="nucleotide sequence ID" value="XM_002837135.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GA29"/>
    <dbReference type="FunCoup" id="D5GA29">
        <property type="interactions" value="847"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GA29"/>
    <dbReference type="GeneID" id="9187070"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003545001"/>
    <dbReference type="eggNOG" id="KOG2872">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040933_0_0_1"/>
    <dbReference type="InParanoid" id="D5GA29"/>
    <dbReference type="OMA" id="LWLMRQA"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00321"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004853">
        <property type="term" value="F:uroporphyrinogen decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00717">
        <property type="entry name" value="URO-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.210:FF:000004">
        <property type="entry name" value="Uroporphyrinogen decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00218">
        <property type="entry name" value="URO_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038071">
        <property type="entry name" value="UROD/MetE-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006361">
        <property type="entry name" value="Uroporphyrinogen_deCO2ase_HemE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000257">
        <property type="entry name" value="Uroporphyrinogen_deCOase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01464">
        <property type="entry name" value="hemE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21091">
        <property type="entry name" value="METHYLTETRAHYDROFOLATE:HOMOCYSTEINE METHYLTRANSFERASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21091:SF169">
        <property type="entry name" value="UROPORPHYRINOGEN DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01208">
        <property type="entry name" value="URO-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51726">
        <property type="entry name" value="UROD/MetE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00906">
        <property type="entry name" value="UROD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00907">
        <property type="entry name" value="UROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 14" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="8" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="9 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="10 14" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Uroporphyrinogen decarboxylase (URO-D)" evidence="16">
        <location>
            <begin position="26"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="Uroporphyrinogen decarboxylase (URO-D)" evidence="17">
        <location>
            <begin position="148"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00052550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00058098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS00906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS00907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAZ81372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="363" mass="40598" checksum="B9B0CB96255D7444" modified="2010-06-15" version="1">MAETFEPLSNDLILRAARGEPVERPPMWVMRQAGRYLPEYNQVKGGRDFFSVCRDPELASTITLQPILRYANLIDAAIIFSDILVIPQAMGMTVEMLEAKGPHFPNPLRKPEDLSQLKPEVDVRAELAYVFDAIRLTRRKLAGRVPLIGFVGAPWTLMSYMVEGGGSKTFMFVKTWIFKHPSATHSLLARITDVCVDFLAYQVLAGAQMLQVFDSWAGELSPHDFRTFSLPYIRQISVRVPARVRELGGEPVPMTIFAKGAWYALDELCESGYQVVGLDWTHDPESAVAIANGRVTLQGNMDPNVLYGGREAITRTVERIAKGFGGGRRGWIVNLGHGITPLVDPEDLRWFFEETRRVTGASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAX0</accession>
    <name>D5GAX0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00005341001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430086">
        <property type="protein sequence ID" value="CAZ81663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837472.1">
        <property type="nucleotide sequence ID" value="XM_002837426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAX0"/>
    <dbReference type="STRING" id="656061.D5GAX0"/>
    <dbReference type="GeneID" id="9187831"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005341001"/>
    <dbReference type="eggNOG" id="KOG2202">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059852_1_0_1"/>
    <dbReference type="InParanoid" id="D5GAX0"/>
    <dbReference type="OMA" id="MIDTRQA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000243">
        <property type="term" value="C:commitment complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0089701">
        <property type="term" value="C:U2AF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12538">
        <property type="entry name" value="RRM_U2AF35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000066">
        <property type="entry name" value="Splicing factor u2af 23 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009145">
        <property type="entry name" value="U2AF_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12620">
        <property type="entry name" value="U2 SNRNP AUXILIARY FACTOR, SMALL SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01848">
        <property type="entry name" value="U2AUXFACTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="9">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="44"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="6">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="188"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ81663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="212" mass="24592" checksum="0046459ADDB94794" modified="2010-06-15" version="1">MANYLASIFGTEQDKVNCSFYYKIGACRHGDRCSRKHVKPSYSQTILLPNLYQNPAFDPKNKMNTSQLQNHFDAFYEDFFCEMCKYGEVEEVVVCDNNNDHLIGNVYARFKYEEDAQTACDALNARWYAARPIYCELSPVTDFREACCRLNSGEGCVRGGFCNFIHRKEPSKDLERELELGMKKWLRARGRDRRSVSRSVSPNGESRRRKEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>D5GB51</accession>
    <name>D5GB51_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00005451001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430086">
        <property type="protein sequence ID" value="CAZ81744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837553.1">
        <property type="nucleotide sequence ID" value="XM_002837507.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GB51"/>
    <dbReference type="FunCoup" id="D5GB51">
        <property type="interactions" value="585"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GB51"/>
    <dbReference type="GeneID" id="9181455"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005451001"/>
    <dbReference type="eggNOG" id="KOG4177">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009666_2_0_1"/>
    <dbReference type="InParanoid" id="D5GB51"/>
    <dbReference type="OMA" id="IHHAAIM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030907">
        <property type="term" value="C:MBF transcription complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033309">
        <property type="term" value="C:SBF transcription complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048315">
        <property type="term" value="P:conidium formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030435">
        <property type="term" value="P:sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.260.10">
        <property type="entry name" value="Transcription regulator HTH, APSES-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036887">
        <property type="entry name" value="HTH_APSES_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018004">
        <property type="entry name" value="KilA/APSES_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051642">
        <property type="entry name" value="SWI6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003163">
        <property type="entry name" value="Tscrpt_reg_HTH_APSES-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43828">
        <property type="entry name" value="ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43828:SF15">
        <property type="entry name" value="TRANSCRIPTION FACTOR MBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04383">
        <property type="entry name" value="KilA-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01252">
        <property type="entry name" value="KilA-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54616">
        <property type="entry name" value="DNA-binding domain of Mlu1-box binding protein MBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51299">
        <property type="entry name" value="HTH_APSES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0183">Conidiation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0749">Sporulation</keyword>
    <feature type="domain" description="HTH APSES-type" evidence="8">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="226"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="347"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="78"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="397"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="482"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="583"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="97"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="131"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="147"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="417"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ81744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="660" mass="73609" checksum="39F9E3AFA71B8984" modified="2010-06-15" version="1">MRRRSDSWVNATQVLKVAEFDKPQRTRILEREVQRGLHEKVQGGYGKYQGTWVPLERAREIAALYNVEELLKPIFDFRPSTESPPLAPKHVTAASTKPRPSRAQPRAPAAPQVKRVVKPTPPVQAPPSIDSLDEVDVEAEDGDLSDLDPLNDPSSPSNSVSATLKRKREQMDNEDILGGFMPSSGQRKVAYSDELLDYFMANEKGLPEFLVNPPPDFDVNEVIDDEGHTAFHWAASMGDLRVIELLYQAQANIFMVNKRGETPLMRAVLFTNNYDRKSFPRLVETLRDTIFHVDSYNSTVFHHIAATTSSRNKLLAARYYFDVLLQKLGEKHSMPDIAMVLDLRDSLGDTALTIAARNGAKKCVRSLLAHNANPNTPNNEGQTADQYIVAVERQRRNGDYPPASSSSPFQSHEHHPPHIHNSRFHRHPSTQSVATVAAINYANNNYIPQPHISEAAIAATQKVIPLMAEMLEGLATAFDKELNDKEEDHSQAQRLLDSSTHEIQTCKATSEETLAPFGDIATMEAQIEALKAEADALAEQLKEVFECGQYHDLSGLVREEAEQLPPQEMEALDEDTSEAWLEKSEVARELSQLQQERAELRDTIVKAYARSGAGERMSDYRRLIALSCSLLPEDVEKLLPEILKDLEASGDGGIGDGLMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>D5GB60</accession>
    <name>D5GB60_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00005464001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430086">
        <property type="protein sequence ID" value="CAZ81753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837562.1">
        <property type="nucleotide sequence ID" value="XM_002837516.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GB60"/>
    <dbReference type="STRING" id="656061.D5GB60"/>
    <dbReference type="GeneID" id="9181464"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005464001"/>
    <dbReference type="eggNOG" id="KOG1337">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044629_0_0_1"/>
    <dbReference type="InParanoid" id="D5GB60"/>
    <dbReference type="OMA" id="AWYRSRC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10527">
        <property type="entry name" value="SET_LSMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1410.10">
        <property type="entry name" value="set domain protein methyltransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050600">
        <property type="entry name" value="SETD3_SETD6_MTase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271:SF76">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271">
        <property type="entry name" value="UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="463"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="468"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="482" mass="53867" checksum="A5381F51FC756D57" modified="2010-06-15" version="1">MHYETLPTSSLAPFIRLNNISLNGVEIARIGGEPIDAGVGVKHAPTAYDPSRPFLNIDAELVVCAKTVEGHAKADVHLAEALAAAGEFTLAPRGAIMVFLVMSMSTAAGLGIGRSGAWSEYIKFLPAVSIPTTWTETERKLLRGTSLDVAVTAKLQALEQEFEDFREATKEIKWCQKAWWDESKMTLRDWQTADSWFRSRVLQFPRLGIGMAPVLDFANHCFEANSYYSVDEDSHVVLLPREDRAGTLKHGEEITINYGQEKSAAEMVFSYGFLDPSKDMALWLVLDLQGLPDDPLGFAKQAVFKSAPTLGIYDLGHTVEWASHFIWLMCVNEEDGLSFRVLQETTGERHLQVLWDSKEVEDIAKLVDLLGDSSLWPIYQLRATALVQDRVETQLARLTESEEEIAWITDQESFNIGSQELHVATRLRTLERALLEKALKVLETQKERLLGSEVVQTYLMQVQGSSEMSDDPEAPDDEDDLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>D5GB79</accession>
    <name>D5GB79_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00003780001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430087">
        <property type="protein sequence ID" value="CAZ81772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837581.1">
        <property type="nucleotide sequence ID" value="XM_002837535.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GB79"/>
    <dbReference type="FunCoup" id="D5GB79">
        <property type="interactions" value="522"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GB79"/>
    <dbReference type="GeneID" id="9181482"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003780001"/>
    <dbReference type="eggNOG" id="KOG0776">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014015_1_0_1"/>
    <dbReference type="InParanoid" id="D5GB79"/>
    <dbReference type="OMA" id="SHNGEIW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990234">
        <property type="term" value="C:transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046165">
        <property type="term" value="P:alcohol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043386">
        <property type="term" value="P:mycotoxin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001:SF69">
        <property type="entry name" value="ALL TRANS-POLYPRENYL-DIPHOSPHATE SYNTHASE PDSS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001">
        <property type="entry name" value="GERANYLGERANYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ81772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="343" mass="37110" checksum="66AE66831273BE41" modified="2010-06-15" version="1">MSRATAVAPKRQSWRRASAELEKIFNNTVAPAEILRDTNPDLALLSSRLMPADILGPGEVAAGTTGERVQVLGTQRRLAEIVELIHTATLLHDDVLDQAETRRGNPSANSLSGNKLSILAGDYMLGRASVYLARLRNPEVIELLAEVIAELVKGELLQLQNTATGTTADFTSKSSREAHLRDSLEYYLEKTYLKSASLISKSCRATAVLGGATEDVAAKAYSYGRHLGLAFQLVDDVLDYTSSNDQIGKPAGADLELGLATAPVFYACEEFGELGELIERRFCLDGDAEKARALVHKSNGIDRTLALAGSFCDTARQAISSFPPSEAKDGLEEVLEMVLTRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>D5GB94</accession>
    <name>D5GB94_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Endo-chitosanase</fullName>
            <ecNumber evidence="10">3.2.1.132</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00000527001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Chitosanase catalyzing the endo-type cleavage of chitosan, the deacylated form of chitin. Chitosanase may be crucial in the degradation of the deacetylated portion of chitin in the fungal cell wall.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>Endohydrolysis of beta-(1-&gt;4)-linkages between D-glucosamine residues in a partly acetylated chitosan.</text>
            <dbReference type="EC" id="3.2.1.132"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the glycosyl hydrolase 75 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.132" evidence="10"/>
    <dbReference type="EMBL" id="FN430089">
        <property type="protein sequence ID" value="CAZ81787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837596.1">
        <property type="nucleotide sequence ID" value="XM_002837550.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GB94"/>
    <dbReference type="GeneID" id="9181498"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000527001"/>
    <dbReference type="eggNOG" id="ENOG502S39Y">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046555_1_0_1"/>
    <dbReference type="InParanoid" id="D5GB94"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016977">
        <property type="term" value="F:chitosanase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009939">
        <property type="entry name" value="Chitosanase_fungal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42061">
        <property type="entry name" value="ENDO-CHITOSANASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42061:SF6">
        <property type="entry name" value="ENDO-CHITOSANASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07335">
        <property type="entry name" value="Glyco_hydro_75"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8 10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 10" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ81787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="144" mass="15208" checksum="42ADD8E8803815E8" modified="2010-06-15" version="1">MDVDCDGANRSDGKCVNDPTGQGQTAFKDGVAKYGLDDLDSNKHSYIVFGNQKYSTSFDPTQYGVPELGVVVVVCAGQFFYAVWGDTNGGTLVGEASISLATACFGQGMTGNSGHPDTDVLYLVFTGNRAATNSTVDWYPLTPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBK6</accession>
    <name>D5GBK6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00005678001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ82012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837821.1">
        <property type="nucleotide sequence ID" value="XM_002837775.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBK6"/>
    <dbReference type="FunCoup" id="D5GBK6">
        <property type="interactions" value="390"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GBK6"/>
    <dbReference type="GeneID" id="9185447"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005678001"/>
    <dbReference type="eggNOG" id="KOG1172">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002289_7_2_1"/>
    <dbReference type="InParanoid" id="D5GBK6"/>
    <dbReference type="OMA" id="RRAPFYW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080139">
        <property type="term" value="F:borate efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005452">
        <property type="term" value="F:solute:inorganic anion antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006820">
        <property type="term" value="P:monoatomic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050801">
        <property type="term" value="P:monoatomic ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011531">
        <property type="entry name" value="HCO3_transpt-like_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003020">
        <property type="entry name" value="HCO3_transpt_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453">
        <property type="entry name" value="ANION EXCHANGE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453:SF82">
        <property type="entry name" value="BORON TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00955">
        <property type="entry name" value="HCO3_cotransp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="59"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="168"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="202"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="254"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="296"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="365"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="393"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="436"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="Bicarbonate transporter-like transmembrane" evidence="7">
        <location>
            <begin position="1"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Bicarbonate transporter-like transmembrane" evidence="7">
        <location>
            <begin position="172"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="500"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ82012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="520" mass="57141" checksum="894E053C6CBD02BB" modified="2010-06-15" version="1">MVNDVRRRLPYYKSDWTDAWDYRIVPSTGLSCLLSSVQYLTGWWCRFEKTKMSYGVNEVLLASVLGAVVFSFLAAQPLCIVGVTGPITVFNYTVYAIVAPRGIPYFQFMCWICLWSMLMHFALAITNACNAVKWVTRFSCDIFGFYVAFIYLQKGIQVLALQWRAGDAAAHLSISISLLVLMSGYACSWLGSSNLFRQPVRVFLKDYGTPLSIVFFTGYQYFGKIKHMDLERLPVSQAFFPTTERGWLVHFWDISVGQVFLAIPFALLLTILFYFDHNVSSLISQGTEFPLKKPAGFHWDIFLLGITTGISGVLGIPAPNGLIPQAPFHTASLCVTRRRTDGAEENKGKVEVVVDHVVEQRASNLAQGLLILGTMTGPLLVVLALIPQGVLAGLFFVMGLQALESNGIVLKLLFLLSDSSLAFGEEPLKRCRKGAVWAFVALELIGFGATFAITQTIAAIGFPVFILLLIPARTLILPKYFTPEELGVLDAPTASPFTLESVSGGNYGGAPEPSGNSTPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBM5</accession>
    <name>D5GBM5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidyl-prolyl cis-trans isomerase</fullName>
            <ecNumber evidence="6">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00005712001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PpiC/parvulin rotamase family. PIN4 subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="6"/>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ82031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837840.1">
        <property type="nucleotide sequence ID" value="XM_002837794.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBM5"/>
    <dbReference type="STRING" id="656061.D5GBM5"/>
    <dbReference type="GeneID" id="9185466"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005712001"/>
    <dbReference type="eggNOG" id="KOG3258">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090028_2_0_1"/>
    <dbReference type="InParanoid" id="D5GBM5"/>
    <dbReference type="OMA" id="IDIVTHV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043323">
        <property type="entry name" value="PIN4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000297">
        <property type="entry name" value="PPIase_PpiC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45995">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00639">
        <property type="entry name" value="Rotamase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50198">
        <property type="entry name" value="PPIC_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 6" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PpiC" evidence="8">
        <location>
            <begin position="45"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="33"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ82031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="147" mass="16016" checksum="26CB689921F27414" modified="2010-06-15" version="1">MGKSKTDNKSSSKSSNSKDKSKDASSSSSKTSAKGDSKSDKLKPANSINVRHILCEKHSKMETVLERLANGEKFDAVARELSEDKARQGGSLGWKTRGSLLKAFEDAAYALAVSTVDRPVYTNPAVKTSEGWVHTLYFEVCRAHFAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5GC07</accession>
    <name>D5GC07_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00005750001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 81 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="3"/>
    <dbReference type="EMBL" id="FN430098">
        <property type="protein sequence ID" value="CAZ82050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837859.1">
        <property type="nucleotide sequence ID" value="XM_002837813.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GC07"/>
    <dbReference type="STRING" id="656061.D5GC07"/>
    <dbReference type="GeneID" id="9181319"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005750001"/>
    <dbReference type="eggNOG" id="KOG2254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005482_2_0_1"/>
    <dbReference type="InParanoid" id="D5GC07"/>
    <dbReference type="OMA" id="WCLPHHQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052861">
        <property type="term" value="F:endo-1,3(4)-beta-glucanase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1170">
        <property type="entry name" value="glycoside hydrolase family 81 endo-[beta] glucanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.30">
        <property type="entry name" value="Golgi alpha-mannosidase II, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005200">
        <property type="entry name" value="Endo-beta-glucanase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040720">
        <property type="entry name" value="GH81_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040451">
        <property type="entry name" value="GH81_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31983">
        <property type="entry name" value="ENDO-1,3(4)-BETA-GLUCANASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31983:SF0">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-D-GLUCOSIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17652">
        <property type="entry name" value="Glyco_hydro81C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03639">
        <property type="entry name" value="Glyco_hydro_81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52008">
        <property type="entry name" value="GH81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 81 N-terminal" evidence="9">
        <location>
            <begin position="2"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 81 C-terminal" evidence="10">
        <location>
            <begin position="321"/>
            <end position="679"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ82050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="697" mass="78268" checksum="3882B3E97E8D2B6B" modified="2010-06-15" version="1">MPYNLAWMNGTRKGLLGLAADTIDLTKRKFGPKNGPGGAVEFYSNAFNPRIILGSTELRKGVSDIRVFDPAGQSARVRIYADANGYNTGIPDEGNATKIIDIPICNGMGFVSAEYHELTPVISSIVAVEDIRQLPSGNELITKYIIRFADDADSWLVYAHHRSIEEAKSTPFRLQSVGTTRLEARYLFTGLLQVAHLSLDNRGHAGREALLDYTTGSYCAGVEINAQITGPYADSGEYQMRFKRAGQRKSPLLMYALPHHVKTFHNSTVANIHRVFKLPSPANGMMYGVIADTWRLKVESLPVEIGWLPLGQEGCASFPPDVLQQIHTVARIEVGQNLTLQTHANPSMYFSGKAYAKYAFLCYSTLHVLKDPGLTRLCLSRLKNDFALFAENRQKNKMVYDTTWGGIVSIRAFEGAEPSGDIDFGNYLYNDHHFHYSYHIQAAALIVQLDKDLGNSNTWFRGNREWVNSLLRDYANPSKEDRYFPVFRNFDWYHGHSWARGLLSCGDSKDEESSSEDLNSLYAQKLWGIAIDDRSMIARANLMLGILKVSTNEYMLMSKGNQNHPKEFVGNLVTGILAESKADHKTYFGSYKEYIQGIHMIPVTAMSPYIRSAEFCELEYQTYFSKGLHYGKSGADKVWAGIIEANRAIFDPKRSWEFFNSRVFDDTMIDGGTSRTWFMILSAGMGGLNINETCHIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCC1</accession>
    <name>D5GCC1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00005811001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Golgi membrane protein involved in vesicular trafficking.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TVP18 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430109">
        <property type="protein sequence ID" value="CAZ82164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837973.1">
        <property type="nucleotide sequence ID" value="XM_002837927.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCC1"/>
    <dbReference type="FunCoup" id="D5GCC1">
        <property type="interactions" value="37"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GCC1"/>
    <dbReference type="GeneID" id="9184841"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005811001"/>
    <dbReference type="eggNOG" id="ENOG502S3AC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118698_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCC1"/>
    <dbReference type="OMA" id="IYAQWLG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019365">
        <property type="entry name" value="TVP18/Ca-channel_flower"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13314">
        <property type="entry name" value="CALCIUM CHANNEL FLOWER HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13314:SF2">
        <property type="entry name" value="CALCIUM CHANNEL FLOWER HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10233">
        <property type="entry name" value="Cg6151-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01077">
        <property type="entry name" value="Cg6151-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="113"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ82164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="152" mass="16327" checksum="98F88010B18FF9C3" modified="2010-06-15" version="1">MGIVESIGSEFKTRNFSIYGQWTGIIAILLCIAVGIANVFHASLLIIFSVICLVSGLLLIFIEIPLLLRICPTSKKFDDFVRKFHSNYGRAGIYLIMATVQWVSIVIDASSLIAAAVVLTFAGCFYGLAGLKGQEFMGSKTLGGAGVAQMIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCD2</accession>
    <name>D5GCD2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00005826001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430109">
        <property type="protein sequence ID" value="CAZ82175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837984.1">
        <property type="nucleotide sequence ID" value="XM_002837938.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCD2"/>
    <dbReference type="FunCoup" id="D5GCD2">
        <property type="interactions" value="1289"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GCD2"/>
    <dbReference type="GeneID" id="9184852"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005826001"/>
    <dbReference type="eggNOG" id="KOG1763">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042870_1_0_1"/>
    <dbReference type="InParanoid" id="D5GCD2"/>
    <dbReference type="OMA" id="AMIFKPV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.400.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032378">
        <property type="entry name" value="ZC3H15/TMA46_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12681:SF0">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12681">
        <property type="entry name" value="ZINC FINGER-CONTAINING PROTEIN P48ZNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16543">
        <property type="entry name" value="DFRP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="92"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="169"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="92"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="169"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="123"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="261"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="21"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="50"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="123"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="261"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="297"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ82175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="335" mass="37701" checksum="48FF44B4717A8797" modified="2010-06-15" version="1">MPPKKDVKNAKPSVQKVVQDKASPQQKKGGKAQKQIAQLASQASASGTPEQKRKEAEKAQREAEKKAAEAAKKEVAELFKPVQTQKVPFGVDPKSVLCVYYKNGNCEKGRKCKFSHDLSIERKSQKKNLYEDSRGEEAEDEKKKEETSDNWDEEKLRSVVLSKHGNPKTTTDKVCKHFIEAVENGKYGWFWQCPNGGNDCKYKHSLPPGFVLKTKEQRAAEKALMDKSPLATLTLEDFLEARRHELTGALTPVTEETFKKWKSERMSKKEAEEEARRAKEATGRALFEKGDWRNGSEDEESSGEDDGAFDLNALRQETQALEGEGDSNLKRYGST</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCE9</accession>
    <name>D5GCE9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00005857001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430109">
        <property type="protein sequence ID" value="CAZ82192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838001.1">
        <property type="nucleotide sequence ID" value="XM_002837955.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCE9"/>
    <dbReference type="GeneID" id="9186374"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005857001"/>
    <dbReference type="HOGENOM" id="CLU_2623789_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCE9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ82192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="78" mass="8648" checksum="1D95D0306313A0BE" modified="2010-06-15" version="1">MDGGIGTSSMGYYMAGLEVFTYQSVEEIRVGWDWVIVEMLEVGGECSRVEDVVEAVLAIWKQNSTLGLGQRGWCDIIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCF2</accession>
    <name>D5GCF2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DASH complex subunit ASK1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">GSTUM_00005863001</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
            <location evidence="3">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DASH complex ASK1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430109">
        <property type="protein sequence ID" value="CAZ82195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838004.1">
        <property type="nucleotide sequence ID" value="XM_002837958.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCF2"/>
    <dbReference type="STRING" id="656061.D5GCF2"/>
    <dbReference type="MoonProt" id="D5GCF2"/>
    <dbReference type="GeneID" id="9186377"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005863001"/>
    <dbReference type="eggNOG" id="ENOG502RZQN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_625697_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCF2"/>
    <dbReference type="OMA" id="ARFWKQF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042729">
        <property type="term" value="C:DASH complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008608">
        <property type="term" value="P:attachment of spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013964">
        <property type="entry name" value="DASH_Ask1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28200">
        <property type="entry name" value="DASH COMPLEX SUBUNIT ASK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28200:SF1">
        <property type="entry name" value="DASH COMPLEX SUBUNIT ASK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08655">
        <property type="entry name" value="DASH_Ask1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="16" id="KW-0137">Centromere</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="11" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="98"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="146"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="231"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="265"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="371"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="98"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="113"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="236"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="307"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="324"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAZ82195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="401" mass="43650" checksum="8363A973293524DD" modified="2010-06-15" version="1">MSRPPLSLTAELEKLEQSITLTLQEIDHNFSKAHRIVTTSIIPIVERYAKESEAVWEGSKFWKQFFEASANVALSNYQEEEETYEETGVATNAETYITASSPGNYGEQSTRITQEDDPRHRETQWENIESPFDALRKDDDADMTFEPTLLPATPQTSRRTRPAKGSFETPKSSPFYPSAAGAIGKKTPGGANEDQLLHRVLDKNWRLQATPLGKPPPSRYRTIGAATATTPKAQILPPPGSESPMSSPPKPHFYSADIFSSPIPGFGGFDGGKKPKPSTTSNTTVLAGDPKTPISRRYGTAKVTTTHHHELSQQGDEGRFAYGYDDDDDSDDLDLPPGLSPPVTIQFSLPPSKLLATPAREASRRIVHDILQTAGAADESGATGGSSPPVVRDVGPLDDTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCF8</accession>
    <name>D5GCF8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Carnitine O-acetyltransferase, mitochondrial</fullName>
            <ecNumber evidence="16">2.3.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">GSTUM_00005870001</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Carnitine acetylase is specific for short chain fatty acids. Carnitine acetylase seems to affect the flux through the pyruvate dehydrogenase complex. It may be involved as well in the transport of acetyl-CoA into mitochondria.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>(R)-carnitine + acetyl-CoA = O-acetyl-(R)-carnitine + CoA</text>
            <dbReference type="Rhea" id="RHEA:21136"/>
            <dbReference type="ChEBI" id="CHEBI:16347"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57589"/>
            <dbReference type="EC" id="2.3.1.7"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Matrix side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 19">Belongs to the carnitine/choline acetyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.7" evidence="16"/>
    <dbReference type="EMBL" id="FN430109">
        <property type="protein sequence ID" value="CAZ82201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838010.1">
        <property type="nucleotide sequence ID" value="XM_002837964.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCF8"/>
    <dbReference type="FunCoup" id="D5GCF8">
        <property type="interactions" value="183"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GCF8"/>
    <dbReference type="GeneID" id="9186383"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005870001"/>
    <dbReference type="eggNOG" id="KOG3717">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013513_5_1_1"/>
    <dbReference type="InParanoid" id="D5GCF8"/>
    <dbReference type="OMA" id="KMDGTPT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004092">
        <property type="term" value="F:carnitine O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009437">
        <property type="term" value="P:carnitine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.70:FF:000007">
        <property type="entry name" value="Carnitine O-acetyltransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.70">
        <property type="entry name" value="Choline/Carnitine o-acyltransferase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000542">
        <property type="entry name" value="Carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039551">
        <property type="entry name" value="Cho/carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042231">
        <property type="entry name" value="Cho/carn_acyl_trans_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589:SF103">
        <property type="entry name" value="CARNITINE O-ACETYL-TRANSFERASE, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589">
        <property type="entry name" value="CARNITINE O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00755">
        <property type="entry name" value="Carn_acyltransf"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00439">
        <property type="entry name" value="ACYLTRANSF_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00440">
        <property type="entry name" value="ACYLTRANSF_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 19" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Choline/carnitine acyltransferase" evidence="20">
        <location>
            <begin position="75"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="Choline/carnitine acyltransferase" evidence="20">
        <location>
            <begin position="407"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="18">
        <location>
            <position position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00052702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00053195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00066910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00073438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR600542-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU003801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="CAZ82201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="621" mass="70724" checksum="5CB2EEF912FD9FDD" modified="2010-06-15" version="1">MFSNVFSRGFQVKSITSASLRHRSVPQKQPAKVVSAVAKLSSTGLARQNMAPPTTYQEDPYAGPMLRFQKFLPKLPVPTLQETADRYVKSLKPLLTEQELQRTKTAVAEFIREGGVGQQLQEKLLAKAQNPEVNNWLSDWWNDAAYLSYRDPVVPYVSYFYSYKDDKKRRHPVKRAAAITTSVLKFKKMVDEKTLEPEYMRGLPICMDSYRWMFNACRIPEKPADYPVKYDYESNKHFVVARKNRFYKVLHEVDGVQLSTGELEAQFARVLELANAEKVPAVGALTSENRDTWTEVRKMMIEASPENKKALQLIESASFIVCLDDTSPVTLEERAHQYWHGDGQNRFFDKPLQFIINENGTAGFMGEHSMMDGTPTHRLNDYLNDQIFHNKIDLAVGDVRSNLPEPLKVLAYLGYGKGLIKKFKCSPDAYVQMVIQLAYYKMYGKNRPTYESAATRKFQVGRTETCRTVSEESVAWCKSMMDPDTDPKTCISNFRAAINAHVVYISTASEGKGVDRHLFGLKKLLPPGAALPKIYADPAYGYSSSWFLSTSQLSSEYFNGYGWSQVIDQGYGIAYMINENNLQFNIVSKKQGCERMQFYLTDAANDLRELMESELETKAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCX6</accession>
    <name>D5GCX6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00000854001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430128">
        <property type="protein sequence ID" value="CAZ82369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838178.1">
        <property type="nucleotide sequence ID" value="XM_002838132.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCX6"/>
    <dbReference type="GeneID" id="9183832"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000854001"/>
    <dbReference type="HOGENOM" id="CLU_2869280_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCX6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="64" mass="7404" checksum="37775AB929519CE1" modified="2010-06-15" version="1">MHSKTYRHPLSYHLQTDPLPILSLMLRNLSSSHQGYSNLDRVFCSPPHGHHPQRNSAVENSREK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>D5GD18</accession>
    <name>D5GD18_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00000936001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="FN430136">
        <property type="protein sequence ID" value="CAZ82411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838220.1">
        <property type="nucleotide sequence ID" value="XM_002838174.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5GD18">
        <property type="interactions" value="775"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GD18"/>
    <dbReference type="GeneID" id="9183880"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000936001"/>
    <dbReference type="eggNOG" id="KOG1240">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001696_0_1_1"/>
    <dbReference type="InParanoid" id="D5GD18"/>
    <dbReference type="OMA" id="ATNTCRI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071561">
        <property type="term" value="C:nucleus-vacuole junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034271">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034272">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045324">
        <property type="term" value="P:late endosome to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13980">
        <property type="entry name" value="STKc_Vps15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000497">
        <property type="entry name" value="Phosphoinositide 3-kinase regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000342">
        <property type="entry name" value="Serine/threonine-protein kinase VPS15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055231">
        <property type="entry name" value="2AA_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045162">
        <property type="entry name" value="Vps15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17583">
        <property type="entry name" value="PHOSPHOINOSITIDE 3-KINASE REGULATORY SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17583:SF0">
        <property type="entry name" value="PHOSPHOINOSITIDE 3-KINASE REGULATORY SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22956">
        <property type="entry name" value="VPS15-like_hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 10" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="26"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="9">
        <location>
            <begin position="396"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="9">
        <location>
            <begin position="558"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="1086"/>
            <end position="1127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="866"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1429"/>
            <end position="1484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="866"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="904"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="938"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1433"/>
            <end position="1449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ82411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1529" mass="169801" checksum="717E4A031607EA82" modified="2010-06-15" version="1">MGQNYSALPDRNVGGAGIDIAELSDISYEKSLGTSRFLKTIKAKHADGGLVVIKIFLKPTPSFSLEEYRIKLEREQEALADIPNALSHQRIIETERAGYLVRQYLYSSLYDRISTRPFLEEIEKRWIAFQLLCGLRDCHARGVHHGDIKTENVLVTSWNWIYLSDFAKFKPTQLPQDNPADFSYFFDTSGRRICYVAPERFLDAGEISGDASVTDEMDIFSLGCVIAELFLEGTPLFNLSQLFKHRRGEYDPSQTYLGKIEDPEIRSLVEHMINLDPTKRYSAEQYLNLWRRKAFPEYFYSFLHQYIGFITDPNPGRRAASKNGIIPVRLDIPNYQRHSSAVRRRPVSADDGTLIFLSLIASSIRNTSRATARVRACDIFLAFAERITDEAKLDRCLPYLVALLNDDSVIVRVAAVRTVTQLMELVEVVTPVNAHVFPDYILPKLQNFASHNNPLIRATYASCLSSLADSASRFLDMAQALRADGSLPTADPEAEDGSAIGSAFQALFDLSRNDLVIYFQEHSKLLLTDIDSGVRRSFLRSVSRLCVFFGTSKANDVILSHLNTYLNDKDWQLRNAFFETITGVATYVGVTALEEYIMPLMVQSLTDPEEFVVEKVLRSLANMAELGLFQRSKIWELINVVGRFMMHPNIWIRQGAVAFIATASKWLSPADIHCIVNPTVRPFLRADVINISTLTMLEHLQKPVSRHVFDMAVTWATQSKKGLFWQGAQEQRTFSFGAIGDSLPRVPVHDFQPGGFAKVPKNEEDINWIEKLRNLGMGPDDEWKVISLREYIWRMALSKPRLLSNENPPLAKNTISLQDLGITPHTVFFDESQSFFNLNMQTTDAVTGNPRPATIADALLDASRTIDNSARRTPSRLQSKRSTGVDITDIMRSRFNSEAGISHSGSGSPTTSSPAGFDTESAPVSGSSTPLAVPPVTITGSNESSTAASSVENGNPLRRRPSTLSLLARNAEASKAAPETSTVSATAFGRVEHPFAKGGDLSLPKTAETVPEPGEFDFRAAHSYEGNDPHILKLLDSMYLQNYPSDILEFGPTIVPVQRRQPIKKSSGRQAGGGIWKPEGVMVAQYGEHAAAINRVIVAPDHNFFLTASDDGTVKIWDSSRLEKNVTFKARQTYKHGTAGTVQVKALCFVENTRCFVSGASDGTIHVIKVDFQMTATAVKYGKLRLMRNWQLPEKGESAVWMEHFKADNHSILLIATNLSNIHALDLRTMRILYTLKNPVFHGTPTAFCIDRRHNWLVLGTSHGILDMWDLRFQLRLKAWGLPGSTPIHRIAIHPSKGRGKWIVVAGGTGHGELSVWDCEKTQCREVYRAGGGKDSGRGYEPWNVDDENPEQMLSRFATALNHLEPGGSGGVDRGVRAFAVGLDAYEETHETRAPPGFIVSAGADRKIRFWNCSKVEASMVVSGLDMEEPKPTFTSSHPTATLTLNTERPPQPLSGADATGVRGDPSSASGSSKKNKATVKTPRSTVISLQQQQLLKAHLDSILDVAFLEVPYGMIVSADRSGVIFIYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDC0</accession>
    <name>D5GDC0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00006143001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430142">
        <property type="protein sequence ID" value="CAZ82513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838322.1">
        <property type="nucleotide sequence ID" value="XM_002838276.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDC0"/>
    <dbReference type="GeneID" id="9183977"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006143001"/>
    <dbReference type="eggNOG" id="ENOG502T00U">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_897689_0_0_1"/>
    <dbReference type="InParanoid" id="D5GDC0"/>
    <dbReference type="OMA" id="FNDHEDE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="169"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="23"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="310" mass="33778" checksum="E537ACD407C37D38" modified="2010-06-15" version="1">MVLHKNKWDRKATKAHERKLKAAGKVVDGAAAGPVKATEKGVKDGGPVLGAPLAGSDVRGKEKEGEPVVGSRSDSEADEQSGSDEGDSAAFRKRTLQNNAWRYLEEEEVPGAEPVIEDPEPDYARLTIGKEVDFSHPSSSVAAKNNVPLPIEDIDEEFLRESFQASCTFTSNSVPSSSRARQDRDKDGGDRTGRVVRVPKSQFVDVTEKISKQTAAEAFRQRFGTKKAKPRPELEAEEGEDDLDSFFEELDRARDGGESHILSPVFLPAPWGRDLKEIIGWCGGRGFIEGLSKNKEWGLIGGGYKLGSVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDE2</accession>
    <name>D5GDE2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00006169001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I LYR family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430142">
        <property type="protein sequence ID" value="CAZ82535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838344.1">
        <property type="nucleotide sequence ID" value="XM_002838298.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDE2"/>
    <dbReference type="STRING" id="656061.D5GDE2"/>
    <dbReference type="GeneID" id="9183998"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006169001"/>
    <dbReference type="eggNOG" id="KOG3426">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111660_0_1_1"/>
    <dbReference type="InParanoid" id="D5GDE2"/>
    <dbReference type="OMA" id="FWKQTTH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045271">
        <property type="term" value="C:respiratory chain complex I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20266">
        <property type="entry name" value="Complex1_LYR_NDUFA6_LYRM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045299">
        <property type="entry name" value="Complex1_LYR_NDUFA6_LYRM6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016488">
        <property type="entry name" value="NADH_Ub_cplx-1_asu_su-6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12964:SF0">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12964">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE B14 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006643">
        <property type="entry name" value="NDUA6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ82535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="123" mass="14678" checksum="812B1B681E1DBBB9" modified="2010-06-15" version="1">MVINPTNLAKTTRVSTGWPDARSRVIKSYRSWQRAAPEIVKQYLLHLPASAIRAKIRQEFERHRYVQQLPVVDVLITKSNMELQETMNYWKQIPHIMKYFRTEEDPRTRLSSNFMNNFLEGRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDJ9</accession>
    <name>D5GDJ9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00001054001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430147">
        <property type="protein sequence ID" value="CAZ82592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDJ9"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001054001"/>
    <dbReference type="HOGENOM" id="CLU_2335168_0_0_1"/>
    <dbReference type="InParanoid" id="D5GDJ9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="98" mass="11271" checksum="C4391BB2990D4566" modified="2010-06-15" version="1">MRAEAISYYITHHPLLVTVSITICLLCIARHPGLCRSQITKAYPAFFRRVRALEDMCEAKAMEIGIVDILREEIRHERNCVSVRPGKEISRWARSVTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDM2</accession>
    <name>D5GDM2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00001092001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430150">
        <property type="protein sequence ID" value="CAZ82615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838424.1">
        <property type="nucleotide sequence ID" value="XM_002838378.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDM2"/>
    <dbReference type="GeneID" id="9181619"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001092001"/>
    <dbReference type="HOGENOM" id="CLU_592105_0_0_1"/>
    <dbReference type="InParanoid" id="D5GDM2"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ82615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="462" mass="52596" checksum="87BC16297E7DE5B1" modified="2010-06-15" version="1">MIRREVMFRAGMMLVNEMEATAWNPGNLMVGIAIHKNPSVRMVKAVESAKDLLREVAVSDHKLRKMDRINDLQPLANIHNTLLQLGGLISLKQHFYEELQGIKLRLQELQRHMTDNIGQAVAEVHDFICFVTFVNLIHPQNCVLTSKSIKDAEILASKNSSPEALQAFVELRQVYDLQTPVPGYRLSKARELPAITRCAMEQWHKGYFSHAAATGIQVIPFQIIFGWWINYQRKFVSSSQEKITPIVQELINCAYTSEASEPKDKDEKLWMQEDKEAFKAVTNLAMSMTELCKNIPLPEHVKPNPQKEKQLPIYTQFKVEPHVPPIFQEFQKLVAQIYGLFEENSCSTFTIMTKAYYPKVENYLRFSEKDQTTSSVKHLSAATYFFSSLREVPVVVLINKNVGGVVPRKAEVPLLDAVSLAGVDPTIADEELLAEITCKNLPQVGMHFLVRYRGQSHQCPRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDQ5</accession>
    <name>D5GDQ5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein uS2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">RPS0</name>
        <name evidence="10" type="ORF">GSTUM_00006226001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains about 33 different proteins and 1 molecule of RNA (18S). The 60S subunit contains about 49 different proteins and 3 molecules of RNA (25S, 5.8S and 5S). Interacts with RPS21.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6 7">Belongs to the universal ribosomal protein uS2 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430153">
        <property type="protein sequence ID" value="CAZ82648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838457.1">
        <property type="nucleotide sequence ID" value="XM_002838411.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDQ5"/>
    <dbReference type="FunCoup" id="D5GDQ5">
        <property type="interactions" value="1167"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GDQ5"/>
    <dbReference type="GeneID" id="9181653"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006226001"/>
    <dbReference type="eggNOG" id="KOG0830">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058171_0_1_1"/>
    <dbReference type="InParanoid" id="D5GDQ5"/>
    <dbReference type="OMA" id="VKNFFEP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01425">
        <property type="entry name" value="RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000010">
        <property type="entry name" value="40S ribosomal protein S0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03015">
        <property type="entry name" value="Ribosomal_S2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001865">
        <property type="entry name" value="Ribosomal_uS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032281">
        <property type="entry name" value="Ribosomal_uS2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018130">
        <property type="entry name" value="Ribosomal_uS2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027498">
        <property type="entry name" value="Ribosomal_uS2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005707">
        <property type="entry name" value="Ribosomal_uS2_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023591">
        <property type="entry name" value="Ribosomal_uS2_flav_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01012">
        <property type="entry name" value="uS2_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11489">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN SA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16122">
        <property type="entry name" value="40S_SA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00318">
        <property type="entry name" value="Ribosomal_S2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00395">
        <property type="entry name" value="RIBOSOMALS2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52313">
        <property type="entry name" value="Ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00962">
        <property type="entry name" value="RIBOSOMAL_S2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00963">
        <property type="entry name" value="RIBOSOMAL_S2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS2 C-terminal" evidence="9">
        <location>
            <begin position="204"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="241"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ82648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="288" mass="31153" checksum="D4F562780C75DFD2" modified="2010-06-15" version="1">MAPTTTNLFDATTQDIEMLLAAQAHIGAKNLQVHMDPYVWKTRPDGVNIINIGKTWEKIVLAARIIATIDNPADVCAVSARSFGQRAVLKFATHTGATAIAGRFTPGNFTNYITRSFKEPRLIIVTDPRTDAQAIKEASYVNIPVIALCDTDSPTEFVDVVIPTNNKGRHAIGLIWWMLAREVRRLRGIIPSRDAEWDVMVDLYFYRDPEAEEQKAVEEADKVPGAEEVVEGGFAASAGAGDWDAPNTGGTFVPPEATGGGNSWDAEAPAEWAADTVATEVPGATSGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDW6</accession>
    <name>D5GDW6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00006312001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430153">
        <property type="protein sequence ID" value="CAZ82709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838518.1">
        <property type="nucleotide sequence ID" value="XM_002838472.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDW6"/>
    <dbReference type="STRING" id="656061.D5GDW6"/>
    <dbReference type="GeneID" id="9184504"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006312001"/>
    <dbReference type="eggNOG" id="KOG3715">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_671194_0_0_1"/>
    <dbReference type="InParanoid" id="D5GDW6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037521">
        <property type="entry name" value="FLCN/SMCR8_DENN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021713">
        <property type="entry name" value="Folliculin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037520">
        <property type="entry name" value="Folliculin/SMCR8_longin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31441:SF2">
        <property type="entry name" value="FOLLICULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31441">
        <property type="entry name" value="FOLLICULIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11704">
        <property type="entry name" value="Folliculin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51834">
        <property type="entry name" value="DENN_FLCN_SMCR8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDENN FLCN/SMCR8-type" evidence="2">
        <location>
            <begin position="108"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="321"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="367"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="321"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="378"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ82709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="410" mass="43742" checksum="4D4CED17268B12DB" modified="2010-06-15" version="1">MTDTCRSCSMTLPGQLNERLSAPSLASSPSGGAKSPTTTKGNNPTLRTKEVIISSAPFLSSEDEDESSEGQPPSYIRRRRATIDPEHLPGSPNARSYFNSTPASDDEATGGKSLLGSSTHTHHITYLSTRAPSVPSRYSTLRQSCVRTLSCELIPAQTGPIMFGDPLAGYTIAYVFRLADSKARGERRSYALVCICPDQKIVVKSWKYVVAAFEALVHRIKLLAAKKAAEDAQVSPALSNSISSITSRGPDGFLRRRIPGEGGSQKGLAELVGREELFVEVHMCFVKLLGGLVRRYGCWAGSELGLSIGTSGNFVTPVMGRSRAGSVSSSVGDEKPEKKTVTSQTVVEGIEMTGGERAANGAKVTFPPTTAGLSVHDTVTPRAAPSTPRTPAAKREEPTHQRSRSAILIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDX2</accession>
    <name>D5GDX2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00006320001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430153">
        <property type="protein sequence ID" value="CAZ82715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838524.1">
        <property type="nucleotide sequence ID" value="XM_002838478.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDX2"/>
    <dbReference type="STRING" id="656061.D5GDX2"/>
    <dbReference type="GeneID" id="9184510"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006320001"/>
    <dbReference type="eggNOG" id="ENOG502S9GA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_510932_0_0_1"/>
    <dbReference type="InParanoid" id="D5GDX2"/>
    <dbReference type="OMA" id="DIPCTQI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="89"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="353"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="446"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="367"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="449"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ82715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="525" mass="59007" checksum="E67ECC62252BE9B0" modified="2010-06-15" version="1">MSVVSIESSIDRAKKLVRSKIRSMMDECKIRKFAMELHSGLSCYTARESGYREVLRMARKTISFLRPGQRAAASTRGGTTTSKGSRSLGALFFGLPVELQLEVVNKLCFFDLLSLRRTSRKFRELTMHNESHIVRHHIGQWVPLHIMKLYPPPTDVPPTLGYLTDLAYKQRVSTHLALSLARQIVKEMMGGRQSRRMSKEATVYVLNQLRLGMAPLIFALFHFFETYRTRRLEHLWGNDDHDSSMAGLDPLSSLADKMRLQGDIISQYPDNLLLQVHQMYHLLLHLFMRRMSSPSPSVQRTLGCWTRQRPPNQAFAKVLILGGIKEVWHIYRIKGFCRRRKALDKYLRKLDAERTRPPKRKNRSEARATTLQQNKGSSIPPNLDVTAPLHSDHNHPPPPPLSKLDVDDLTQLWTPAAEHRLIFGGIVGSLEEVRCCGQFVSQLLGGVTESDDEEDDADGDDSEDDSDDSADDEASPVLAPGGGNTVYSNTLLSADSSWDDELAEEDSGLFSDDEGLGLGSVLATV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>D5GE26</accession>
    <name>D5GE26_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00006342001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430164">
        <property type="protein sequence ID" value="CAZ82769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838578.1">
        <property type="nucleotide sequence ID" value="XM_002838532.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GE26"/>
    <dbReference type="GeneID" id="9183101"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006342001"/>
    <dbReference type="eggNOG" id="ENOG502ST5U">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_133420_1_0_1"/>
    <dbReference type="InParanoid" id="D5GE26"/>
    <dbReference type="OMA" id="QHIQDLG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031632">
        <property type="entry name" value="SVIP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15811">
        <property type="entry name" value="SVIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="1" id="KW-0519">Myristate</keyword>
    <keyword evidence="2" id="KW-0564">Palmitate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="47"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="58"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="116"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ82769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="125" mass="12562" checksum="A22DC7052C85F8FB" modified="2010-06-15" version="1">MGNLCGKESGSSLPNGRVLGSGAPPTAAASKIPLTSKKPKIASGAGRTLGGGGGSEGGSAEAGAAAGEQLDPRTAAALAAEERSKKRAGTGKLGSELDRQKRKTQNQHIQDLGKTKGQQEQLVWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEA3</accession>
    <name>D5GEA3_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Non-histone chromosomal protein 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00001197001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">DNA-binding protein that induces severe bending of DNA. Required for DNA-binding by the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. Also augments the fidelity of transcription by RNA polymerase III independently of any role in the FACT complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NHP6 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430170">
        <property type="protein sequence ID" value="CAZ82846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838655.1">
        <property type="nucleotide sequence ID" value="XM_002838609.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEA3"/>
    <dbReference type="FunCoup" id="D5GEA3">
        <property type="interactions" value="397"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GEA3"/>
    <dbReference type="GeneID" id="9185502"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001197001"/>
    <dbReference type="eggNOG" id="KOG0381">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082854_10_1_1"/>
    <dbReference type="InParanoid" id="D5GEA3"/>
    <dbReference type="OMA" id="MKNMGGK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01390">
        <property type="entry name" value="HMG-box_NHP6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000016">
        <property type="entry name" value="FACT complex subunit SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050342">
        <property type="entry name" value="HMGB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEIN DSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112:SF22">
        <property type="entry name" value="MITOCHONDRIAL TRANSCRIPTION FACTOR A, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00886">
        <property type="entry name" value="HIGHMOBLTY12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="8">
        <location>
            <begin position="23"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="6">
        <location>
            <begin position="23"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="65"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="65"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00057588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00067275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ82846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="103" mass="11680" checksum="0D505B1584DC4FBA" modified="2010-06-15" version="1">MPRTERIPQPAQAAQKKKDPNAPKRGLSAYMFFANEQRENVRNDNPGIAFGQVGKVLGERWKALSEKQRQPYEAKAAADKKRYEDEKAAYNAQGGPEEEEDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEA9</accession>
    <name>D5GEA9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribonuclease H2 subunit B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Ribonuclease HI subunit B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00001207001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Non catalytic subunit of RNase H2, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430170">
        <property type="protein sequence ID" value="CAZ82852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838661.1">
        <property type="nucleotide sequence ID" value="XM_002838615.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEA9"/>
    <dbReference type="STRING" id="656061.D5GEA9"/>
    <dbReference type="GeneID" id="9185508"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001207001"/>
    <dbReference type="eggNOG" id="ENOG502SAQ3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057573_0_0_1"/>
    <dbReference type="InParanoid" id="D5GEA9"/>
    <dbReference type="OMA" id="YYFCPKL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032299">
        <property type="term" value="C:ribonuclease H2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09270">
        <property type="entry name" value="RNase_H2-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040456">
        <property type="entry name" value="RNase_H2_suB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019024">
        <property type="entry name" value="RNase_H2_suB_wHTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041195">
        <property type="entry name" value="Rnh202_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13383">
        <property type="entry name" value="RIBONUCLEASE H2 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13383:SF11">
        <property type="entry name" value="RIBONUCLEASE H2 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09468">
        <property type="entry name" value="RNase_H2-Ydr279"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17745">
        <property type="entry name" value="Ydr279_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rnh202 triple barrel" evidence="9">
        <location>
            <begin position="11"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="Ribonuclease H2 subunit B wHTH" evidence="8">
        <location>
            <begin position="97"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="224"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="303"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00033464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF09468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ82852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="382" mass="42842" checksum="1022F9CB2D6EC211" modified="2010-06-15" version="1">MSPNKPPHIILLPSTVPSTDLKIITLPHPHTLVPTRYLVHPTHGLHEIIKLTAPTSTPRSWLLTPPSKLSSPPKWLRNGQILQDGSMYICTLMDPLFVLLPRLFPTGAVDVVNMFLPVDDLLEEITKGEEGDWEFVIKSQVTERRLAAVCESVDIGGRKAYKPSREKLLKVLHGKCVRMARGELPATMEEEFVRKPLARPVAEIMAELAEEREGARSVKELEDNVGERGGVDGTEAIRMGSPALPTPSDSQSRQPRLAEASLEISDLLRIRVASEFISNSYLPVCLAEVLSDCLRKSHDFTPLDNYLAELVKIRQEATAARNDYTLKRSHSNEEIVDRKRRKKEVEAVEKKKRNVSRAAKELQMVDKKGMPKLTAFFKKKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEF1</accession>
    <name>D5GEF1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00006454001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430175">
        <property type="protein sequence ID" value="CAZ82894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838703.1">
        <property type="nucleotide sequence ID" value="XM_002838657.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEF1"/>
    <dbReference type="GeneID" id="9181525"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006454001"/>
    <dbReference type="eggNOG" id="ENOG502RXUE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003667_0_0_1"/>
    <dbReference type="InParanoid" id="D5GEF1"/>
    <dbReference type="OMA" id="VQSYRTN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025256">
        <property type="entry name" value="TM7S3/TM198-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39469">
        <property type="entry name" value="CHROMOSOME 1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39469:SF1">
        <property type="entry name" value="DUF4203 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13886">
        <property type="entry name" value="TM7S3_TM198"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072732" evidence="7">
        <location>
            <begin position="25"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="173"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="203"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="225"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="255"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="TM7S3/TM198-like" evidence="8">
        <location>
            <begin position="125"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="61"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="389"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="439"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="593"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="786"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="61"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="77"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="466"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="492"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="600"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="684"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="738"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="853"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="877"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="946"/>
            <end position="959"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ82894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1099" mass="119823" checksum="5A95F3E922F4E288" modified="2010-06-15" version="1">MYLDRPFVLHALLVLSLFCGIALSGGENIHRRQDGNVALTTSNNRPSADAEVVDQTSISRAATNTASNAPSSTRNAQSSDPSPTSGGKNAGETIKSSPAPTMAESGDNLSLNTNPADEIYQGVAGVLLLLAGIPYCLIGIKIKFLHIFLSTALLASLGVTVLVVYVMNPPVKFAIQGAYVTAATITGLLTGSLAVVFPEVTEALGCLLGGFCVSMWFLVLKPGGLVASVYGKLILIAVFCFALFALAFHRLTKPYSLIFSLSFSGATTIVLGIDCFSRAGLKEFWLYIWALNDNIFPLETDNYPHTRGIRVEIAIIVLITGFGIMSQVKLWNILKERRDAKDAERRRQDADIEAMDADAGLRVEEEHKLEREQWEATFGRKKADEVIAEERPATGRISRTDSGLGDDVGYGKSSIERAEEFVETEEMRREREEHIQMAQMGVSRSQSISESGGGTVKSQGPAKHNQALEDVDKIEPVEGSEAPKHEGAITHGSVSRRQSSQSIAPSYSNQSTPQRLSSQPVVSQEAPVQGPDVPPPPVVIPLPFPIPVEMGNDDDEVSSLATFAGSDRVISQEIDEDAFRSVVKMVDEEDRASSVAATLDDNEDEVVDPAGDDFSPIMEPPQEDSPPHIPLVKIEVEEVLDESRNRDPDPGLVGEEAQVMVEDKSERAESPGSGKSDAPLQNNAEKEPVTEDHSKRTDSPDETTLIAPTSVSTPPSTLPPPVVSTTPVNLAQIRGVLTPASTPKSKSSSPSPSSAETQLSAKCSKVLKNYRTNEWAKHLADAELPELDKLEEAPEAIDAPDETPAPVHIEELQETAAIKPPAPPRPVSKTSSHENPYPMPVPVNRSPAQLGKSRPSIRNSSSPNPLPESVSPVERSMSPTGRATPIPFTQNTLIGKRESMVRSRQSFNALAEHGSTSPAPAPQPPPEPKHHQPMAIPAPNAPFVPQRQSTGPFAQLPTSDNKSAYKYRLNMLDDDMPLADRRNELLQHVVPQAGVAPVPPQPQRRSLPPQVDRREAMLKNWRETVKTELQTSGHATHAIQERRGEMLNERMQSIQNERHKNLKENFRDEMFDERMRQKDMLDLHKEAMRRMQASANKHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5GER5</accession>
    <name>D5GER5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00001343001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430185">
        <property type="protein sequence ID" value="CAZ83008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838817.1">
        <property type="nucleotide sequence ID" value="XM_002838771.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GER5"/>
    <dbReference type="STRING" id="656061.D5GER5"/>
    <dbReference type="GeneID" id="9184920"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001343001"/>
    <dbReference type="eggNOG" id="KOG0469">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002794_8_0_1"/>
    <dbReference type="InParanoid" id="D5GER5"/>
    <dbReference type="OMA" id="LLINNCI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01885">
        <property type="entry name" value="EF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000058">
        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908:SF10">
        <property type="entry name" value="EUKARYOTIC TRANSLATION ELONGATION FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908">
        <property type="entry name" value="TRANSLATION ELONGATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="4">
        <location>
            <begin position="5"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ83008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="346" mass="39074" checksum="984FD1C42AA7050D" modified="2010-06-15" version="1">MDHVTNVRNMSVIAHVDHGKSTLTDSLVQRAGIISAAKAGEQRFTDTRQDEQDRCITIKSTAISLYAELSEEDCKDITQKTEGNHFLINLIDSPGHVDFSSEVTAALRVTDGALVVVDCVEGVCVQTETVLRQALSERIKPVCIINKVDRALLELQVSKEDLYQSFSRTVESVNVIVATYFDKALGDVQVYPYKGTVAFGSGLHGWAFTVRQFAGRYAKKFGVDKNKMMERLWGDNFFNPKTKKWTKTEVHDGKPLERAFNQFILDPIFRIFSAVMNFKKDEIPVLLEKLEITLKSDEKDLEGKALLKVVMRKFLPAADALMEMIVMHLPSPVTAQKYRLYWYPGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5GF54</accession>
    <name>D5GF54_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00001861001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430207">
        <property type="protein sequence ID" value="CAZ83147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838956.1">
        <property type="nucleotide sequence ID" value="XM_002838910.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GF54"/>
    <dbReference type="GeneID" id="9188008"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001861001"/>
    <dbReference type="HOGENOM" id="CLU_2689595_0_0_1"/>
    <dbReference type="InParanoid" id="D5GF54"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="74" mass="8871" checksum="FA5D531A8EF8A9DD" modified="2010-06-15" version="1">MRGYSFHHSATQRRRHWPVTNEPFALPGWRGYNFGKFPISTFFPSFFNYTFPFVVVLYLSFNRNLHTSSYLELS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFR4</accession>
    <name>D5GFR4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00007019001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430220">
        <property type="protein sequence ID" value="CAZ83357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839166.1">
        <property type="nucleotide sequence ID" value="XM_002839120.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFR4"/>
    <dbReference type="GeneID" id="9187277"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007019001"/>
    <dbReference type="eggNOG" id="KOG0065">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_35_1_1"/>
    <dbReference type="InParanoid" id="D5GFR4"/>
    <dbReference type="OMA" id="AKETCEM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03233">
        <property type="entry name" value="ABCG_PDR_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002416">
        <property type="entry name" value="ABC multidrug transporter (Eurofung)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029481">
        <property type="entry name" value="ABC_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034001">
        <property type="entry name" value="ABCG_PDR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010929">
        <property type="entry name" value="PDR_CDR_ABC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19241">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14510">
        <property type="entry name" value="ABC_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06422">
        <property type="entry name" value="PDR_CDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="588"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="618"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="657"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="694"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="724"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="225"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="829"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="22"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="56"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="91"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ83357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="883" mass="97220" checksum="E2E68380986AA4F6" modified="2010-06-15" version="1">MADRPVARPSRTEEGIPGPGGWPSTPGPSSIGGDSSTTAAAPTPNTGTSSTPGTAQESLPSQLLSSTRHESSDKTLANDNILETPPHRANDFSSSSPSDNKLKKTPTTTSISSNPLVTTETIRPVLTNRQNTNLARTTSQLNEDEIMRILSRRKTNFSSVVEDHDPETNAEIEALLSSIFGSSRQQEKARHLGIVFKDVVVKGQGLGAALQPTNGDFFLGPLRAIRGLSSGRKEGGGGHAIRTLIHGFSGCVRPGEMCLVLGRPGSGCSTFLKVIGNQRDGFVEVNGDVTYGGTDSETMKKKYRGEVLYNPEDDLHYATLTVKDTLKFALETRTPGKESRREGESRKDYVKEFLRVVSKLFWIEHTLDTKVGGEIVRGVSGGEKKRVSIAEAMITKASVQCWDNSTKGLDASTAIEYVESLRTLSNMANISTFVALYQAGESLYQLFDKVLLIDEGRCAYFGPTENARAYFEGLGFVCPPRWTTADFLTSVTDRHERQVKQGWEDQVPRNAEELEQAYQQSEIATATQNDIAGFESTVVAQQEERRRLATEKARTKNYAIPFHKQVYACTKRQFLVMIGDPVSLGGKWGGVLFQSLIVGSLFYNLPQDSNGVFPRGGILFFTLLFNALLALAELTAAFYSIPILLKHKSFSFYRPSAYAIALFVVDLPLCFVQVFIWNIVVYFMSGLQRTASQFFISLLVVFVITASMYSFFRMIGAFSASLDVATRITGVAIQALIVYTGYLIPPSSMHPWFSWLRWINPIQYGFEALMANEFYNLNIQCVPPMLVPVGPNVDPRYQSCALQGSQPGTTIVRGEDYIQTQFSYSRAMEKKDVESGGKDPIVTGDEHNPKESTSGVARNESVFTWQGVTYTIPVKVDMSDLVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFS5</accession>
    <name>D5GFS5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00007051001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <dbReference type="EMBL" id="FN430220">
        <property type="protein sequence ID" value="CAZ83368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839177.1">
        <property type="nucleotide sequence ID" value="XM_002839131.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFS5"/>
    <dbReference type="STRING" id="656061.D5GFS5"/>
    <dbReference type="GeneID" id="9184782"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007051001"/>
    <dbReference type="eggNOG" id="KOG0258">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014254_1_1_1"/>
    <dbReference type="InParanoid" id="D5GFS5"/>
    <dbReference type="OMA" id="NPPRENE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000010">
        <property type="entry name" value="Alanine aminotransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045088">
        <property type="entry name" value="ALAT1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11751">
        <property type="entry name" value="ALANINE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11751:SF29">
        <property type="entry name" value="ALANINE TRANSAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ83368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="201" mass="22564" checksum="5C14A6E9B9D924DB" modified="2010-06-15" version="1">MQKASPGVYDEVELVSFQSVSKGMVGECGHRGAYFELVGFDQEVQAQIYKLISIGICPPVIGQCLVEMMVNPPAPGSPSYQLYQQEYSSIFQSLVLRSRALHEAFVRMEGVECQDPLGAMYLFPTITIPTAAVEKAQEEGRRPDEYYCMRLLEATGICVTPGSGFGQREGEWHFRTTFLAPGVDWVARLEKFHEGFMNEFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>D5GG12</accession>
    <name>D5GG12_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00007148001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430231">
        <property type="protein sequence ID" value="CAZ83455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839264.1">
        <property type="nucleotide sequence ID" value="XM_002839218.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GG12"/>
    <dbReference type="STRING" id="656061.D5GG12"/>
    <dbReference type="GeneID" id="9181800"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007148001"/>
    <dbReference type="eggNOG" id="ENOG502S4S5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_359878_0_0_1"/>
    <dbReference type="InParanoid" id="D5GG12"/>
    <dbReference type="OMA" id="LERNQAC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050815">
        <property type="entry name" value="TF_fung"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47338:SF10">
        <property type="entry name" value="TRANSCRIPTION FACTOR DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47338">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="7">
        <location>
            <begin position="74"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="113"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="185"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="260"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="617"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="13"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="44"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="55"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="113"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="185"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="272"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="624"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ83455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="778" mass="83978" checksum="987AAA95823417C2" modified="2010-06-15" version="1">MAGSPGSPPPAPAISVSSPSSAIPAKRPLSDGNRNDHGNGNAVNSHNSSSPPNSTVHASVSTPPTKKVLERNQACLSCRSRKRKCDGGKPTCGACERLERKCQYSQNSSAAVTKIHSSSSASHQTQKASQPLQQWAPNATNEDSQALQIGAPPPRADRSEHVPANTVVASTVASGVAVPNHNTFTPINLESTSQPPMVNGNSGLRHEVTQSCDSSKNSKRPYDNTASNQAGNFACQFLQASPFVGLDAINIVPWGSPAGVGAWDDRPKKNKSLSPQDIETPESTNSAITLVNNDAGGVLEELPSHDTIVELFELFFDQVHPIIPCLYRKQILADIQPGGSLSKPLPLVFVVLALASLIHPAPAVQDRSARWHAAAKRGFDAAVTRGNYSLDNVQASIYICMYCFVTAELSELWIFLGKGYRMSNPLGLHQIDSPRRGRFQGFLPHPKSEQDLEERRRAAWGLYVLDSFCSCSCGWPLAINDRDFCVNFPVDEDVFQSGEIEKMSHIVVEPFAHNLADLIGPTSASSPPRGPFHYLCKLAVLIGRIVSYHNSPIAPTDNTDFESLETTLARFRLSLPRRYRSIVELSPSEVTHVTQLQILLHVCTILLYHPTVPVPTSPLSSSSDGQLHQNRGQSPGSSIHDLSSAEPAFTRCVSAVNNILSILRTHINSPPPCSPTGSLVVIDVLQNPFITPSLFLSARILAVRWLETGRDKGVRAKIELILGVFDRIKEVWPDVASKYRCLVLADMARDGNGLGGIRGGGSGFMGKECAPACVGPDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>D5GG70</accession>
    <name>D5GG70_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00007238001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430242">
        <property type="protein sequence ID" value="CAZ83513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839322.1">
        <property type="nucleotide sequence ID" value="XM_002839276.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GG70"/>
    <dbReference type="GeneID" id="9184575"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007238001"/>
    <dbReference type="HOGENOM" id="CLU_1887265_0_0_1"/>
    <dbReference type="InParanoid" id="D5GG70"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ83513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="135" mass="14537" checksum="B0AF3B0D491C847A" modified="2010-06-15" version="1">MIPPPSCDNLFTLLWRNGGSSDLRDSSKGFSFPFPISASSTVTGIITNSCSSTKSYRPLTPLPPPQHSACSDTGNVCTCTSPSPRTTPSPPSSSPIPIRISCPNVARSRCQKSTFFFFFFLSRLARIAHPQPGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGG4</accession>
    <name>D5GGG4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007374001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430264">
        <property type="protein sequence ID" value="CAZ83607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839416.1">
        <property type="nucleotide sequence ID" value="XM_002839370.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGG4"/>
    <dbReference type="STRING" id="656061.D5GGG4"/>
    <dbReference type="GeneID" id="9183205"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007374001"/>
    <dbReference type="HOGENOM" id="CLU_380439_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGG4"/>
    <dbReference type="OMA" id="PYESTIA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="364"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="548"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="643"/>
            <end position="728"/>
        </location>
    </feature>
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        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="245"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="408"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <begin position="495"/>
            <end position="521"/>
        </location>
    </feature>
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        <location>
            <begin position="559"/>
            <end position="581"/>
        </location>
    </feature>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
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            <begin position="594"/>
            <end position="606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="728" mass="78771" checksum="2C488ACB86F7CC2D" modified="2010-06-15" version="1">MLSTPQKVLRETLRARMSTSTFDLNKVSEGKDIYEDIEPDDDEDNATEPDDPEEEQRGRQMSILTEEKREIRRIRGRESYARRKEASAKLGSTSQAQTQNGDAVLGRLECVAGSEKSTDRLTPRPYRKKRKSDADHAYRPVSSDSESDDEETGTPKRAKRKMSNRRLASTVTGEARANEHFPTPPKKQRMRASDSADTGSTVVSKFIEGSMRDRASAIPPKDIIGLLPSSSPNRHEGEIAYEPAQRQSTDSTGSAAMNSASPSLLGSIGTAAGALNPFKFAHRIKDIWEKQKAEYIAHERKKRMLADRRKRSEKAYEEMKRLGAAQGFTGNAAAVAGANQIRFRYEDYVMAGMDGNHEDIGMALGTDSGDEVSVGDDAAGASEEKGISGVTNSVRSGMAISVEQRNSGSERSESGVRGDHSMRSSLSDHSRASLTFPRASDRRSGSTSGRNARHSRTSSRMLSPPPLAEGAANKLNGVGTLVKKKSKVFVNSIIGKDDKKDDSATKGERGPTKRELKKQERLRNRVSNLEEQLERARQELEIATRGANVPPVPQIPARITNSTVRTVATTDPETPQQQNPRRSNDEATNRSDIAEGEETEVDDNEVDEGFSVVPLSPVMNTSFGGSFNATAMAGMTIIESLGEANHTSTPRNARRPYSLGSGLRTLPKRASTPVLGNLSGGRGNSASAATAGRGVGGVKGARHSPPPPVPDVGSVPPVPRLSRRASGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGG9</accession>
    <name>D5GGG9_TUBMM</name>
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        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007379001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430264">
        <property type="protein sequence ID" value="CAZ83612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839421.1">
        <property type="nucleotide sequence ID" value="XM_002839375.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGG9"/>
    <dbReference type="FunCoup" id="D5GGG9">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GGG9"/>
    <dbReference type="GeneID" id="9183210"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007379001"/>
    <dbReference type="eggNOG" id="ENOG502S6H9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044999_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGG9"/>
    <dbReference type="OMA" id="YAELPFY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052228">
        <property type="entry name" value="Sec_Metab_Biosynth_Oxidored"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47534:SF3">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE-LIKE C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47534">
        <property type="entry name" value="YALI0E05731P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="318" mass="34466" checksum="59CF1FC8E3382BBD" modified="2010-06-15" version="1">MVNITSIRSANADFASLPLAKGLVAVFVGATAGIGESALRTFVSNTVDPTVYFVGRSESAGSKITADLQALNPSSKIQFLQADTTLLSTVDKVTNQIAASEKKLNLLFMSPGYMTMQSRDESPEGIDRKMAISYYARMRFALNLLPLLSTSEPARVISVLGPGTEGALDMKDLGLAAPGSFSLAAVARHTVTMTSLTFEHLSRQYPNIGWIHATPGFVITGLTRGLPAPLRILSKITEPLLKFASTPIGDSGQGFFYLSTQEKFAKGLWLVDWKGESVDSRVEEGKNRWAKSGAQWWGDSKVEAVWRHTEEVMASVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGM9</accession>
    <name>D5GGM9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00007430001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430275">
        <property type="protein sequence ID" value="CAZ83651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839460.1">
        <property type="nucleotide sequence ID" value="XM_002839414.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGM9"/>
    <dbReference type="STRING" id="656061.D5GGM9"/>
    <dbReference type="GeneID" id="9185573"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007430001"/>
    <dbReference type="eggNOG" id="ENOG502S55E">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_321643_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGM9"/>
    <dbReference type="OMA" id="TMEKDDF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056687">
        <property type="entry name" value="DUF7785"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057199">
        <property type="entry name" value="DUF7877"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25009">
        <property type="entry name" value="DUF7785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25289">
        <property type="entry name" value="DUF7877"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7877" evidence="3">
        <location>
            <begin position="118"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7785" evidence="2">
        <location>
            <begin position="584"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="283"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="610"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="720"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="771"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="68"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="296"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="742"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="785"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="830"/>
            <end position="845"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ83651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="901" mass="95736" checksum="04B9698986C6CFA4" modified="2010-06-15" version="1">MGAANSPSPAPVAVQDAAAGNSATTTACASATGNDSAMDASSQQPQQPLFSPASANANSSTGKRKRSGSPSPSASTTSTQHPSPTKKKPDDSKKSAGDSTMITVVEDRASRRLLFMEATMRDLLTLLKSHDPTPSILRHPIPNSMPSPSGAPPIKRTKISPSSPNTLAEKLLRVTYTSLTTLLSDISAVCRELLTSADNERSNGIPTNGTSSIGSCSGLPFVAQVIHFEELAKQVITRERVRRADLFDFVEEASGDSVTTKPVGSRGKVALTVMGQHGPLFSSLQNPVNALPTGDSVPSSRSTSASASPKSSISTVPTSTATSPKAELLNERPVYTPLNESHLPPLISTVKAIPSNGQILRGGKRLPSKEGAKVSTLGEAFPAPRGLPPLLPPKPGQPSTVGLKWGGKIGVGGIRLENPLNDRPEGCGDWLSYKNNLPSNHPLARHEPPRSRAPLPADLVAAYSSFAPSRDDSGSKIPAAVRNRVWWDRAGKQIFAEEFADDFSLHQMHSRSDEKAAVVEAQTEEMDLDEEFRKVIDDWEPDKEIPIDTRLLNGIEGAEAIVDGKKKAGEEGRADVDTAGTEGDPEKILSEISNLLQSLRSQQYTRLSALPVGQPSKPGTGNAPVLGTPLAPDEEEIGLYNIIRDRLIRLISKLPPHLVTTIDGDRSSDLTLSSKLPVLGGPIFKGTLPADESHPPKLSTPQVQGAAMAAMNAAAGLSPVNSAPVAHMSPARSQHHHHHHTPNTSRPMSTLPNISVDAQYHGHQMPHYHGHTIPSVTTPHHYPSPYAPAPSPIPTPTPQGQYQPPPPRIVSPLNHRQPAPQQQFHGHSLPAQTYQNPPPAYQAQPHSRRTGVPPPQGLAAVGVGYSSPTYNNAGLRMGVQHTQQSMGPGAMSGGRYAGRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGS1</accession>
    <name>D5GGS1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">1,3-beta-glucanosyltransferase</fullName>
            <ecNumber evidence="9">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00007490001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Splits internally a 1,3-beta-glucan molecule and transfers the newly generated reducing end (the donor) to the non-reducing end of another 1,3-beta-glucan molecule (the acceptor) forming a 1,3-beta linkage, resulting in the elongation of 1,3-beta-glucan chains in the cell wall.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cell membrane</location>
            <topology evidence="1 9">Lipid-anchor</topology>
            <topology evidence="1 9">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glycosyl hydrolase 72 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="9"/>
    <dbReference type="EMBL" id="FN430275">
        <property type="protein sequence ID" value="CAZ83693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839502.1">
        <property type="nucleotide sequence ID" value="XM_002839456.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGS1"/>
    <dbReference type="FunCoup" id="D5GGS1">
        <property type="interactions" value="133"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GGS1"/>
    <dbReference type="GeneID" id="9185615"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007490001"/>
    <dbReference type="eggNOG" id="ENOG502QPST">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021855_2_1_1"/>
    <dbReference type="InParanoid" id="D5GGS1"/>
    <dbReference type="OMA" id="MTAYFNC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042124">
        <property type="term" value="F:1,3-beta-glucanosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071970">
        <property type="term" value="P:fungal-type cell wall (1-&gt;3)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000038">
        <property type="entry name" value="1,3-beta-glucanosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004886">
        <property type="entry name" value="Glucanosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012946">
        <property type="entry name" value="X8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468:SF2">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03198">
        <property type="entry name" value="Glyco_hydro_72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07983">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00768">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 9" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8 9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005126577" description="1,3-beta-glucanosyltransferase" evidence="9">
        <location>
            <begin position="22"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="domain" description="X8" evidence="10">
        <location>
            <begin position="377"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ83693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="528" mass="56121" checksum="1FC616FD70BCC5BC" modified="2010-06-15" version="1">MWARSLASALIASSLLSTVAAADLDPIVIKGSKFFFKSNGTQFFMKGVAYQASPTNSTSGTFVDPLADEAGCKRDIPLLQELGTNTIRVYAINTTLDHSTCMKLLNDAGIYVVSDLSEPTLSINRNSPEWDDRLYERYTSVVDALAPYSNVLGFFAGNEVSNEANNTNASPFVKAAVRDMKAYIKKRNYRTIPVGYATNDDAKIRSELADYFDCGSSSESVDFWGYNIYSWCGKSSYTESGYDQRTKEFENYNVPSFFAEYGCNVVQPRVFTEVEALYGPQMTPVFSGGIVYMYHQEANDYGLVETSGDGTATKLVDFNNLKSELAKVSPSGVQSNSYNPTNTAARSCPTADSNWSASTALPPTPNRELCSCMVSALSCVAATKVQEKDTGDLFNYICGEAKTDCTGITANGTTGVYGAYSMCSPLERVSWAMNAYYTKQKKASDACDFSGSAKTQTAAAASGTCKSLLSEAGGTAGTGTVTSSPNSSGTGTKGAAAGLTIPAFQVGAFKIGAGLLAAALGGATLVFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGW9</accession>
    <name>D5GGW9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00007556001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family. Cyclin AB subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430286">
        <property type="protein sequence ID" value="CAZ83762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839571.1">
        <property type="nucleotide sequence ID" value="XM_002839525.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGW9"/>
    <dbReference type="STRING" id="656061.D5GGW9"/>
    <dbReference type="GeneID" id="9181733"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007556001"/>
    <dbReference type="eggNOG" id="KOG0653">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020695_2_4_1"/>
    <dbReference type="InParanoid" id="D5GGW9"/>
    <dbReference type="OMA" id="PAIDFCK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044772">
        <property type="term" value="P:mitotic cell cycle phase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20512">
        <property type="entry name" value="CYCLIN_CLBs_yeast_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000005">
        <property type="entry name" value="G2/mitotic-specific cyclin B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046965">
        <property type="entry name" value="Cyclin_A/B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004367">
        <property type="entry name" value="Cyclin_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048258">
        <property type="entry name" value="Cyclins_cyclin-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02984">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001771">
        <property type="entry name" value="Cyclin_A_B_D_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01332">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00292">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3 5" id="KW-0195">Cyclin</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="6">
        <location>
            <begin position="7"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin C-terminal" evidence="7">
        <location>
            <begin position="100"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="6">
        <location>
            <begin position="104"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ83762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="236" mass="27462" checksum="144513CA68576EB5" modified="2010-06-15" version="1">MRGILVDWLIEVHTRFRLLPETLFLAVNIVDRFLSAKIVELDKLQLVGVTAMFIAAKYEEVFSPGVQYFRSVADDGFTEEEILQAERYILTTLNYNLSYPNPMNFLRRISKADQYDYETRTVAKYLLEISLLDHRFMGYAPSHVSAAAMYLSRMMLERGPWDADLVHYSDYTEEEVLPVFRLMIDYLARPVKHEAFFKKYASKKFMKASIHARQWAKRNAGAHGVNIESATHDDSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>D5GH32</accession>
    <name>D5GH32_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00007682001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430297">
        <property type="protein sequence ID" value="CAZ83825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839634.1">
        <property type="nucleotide sequence ID" value="XM_002839588.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GH32"/>
    <dbReference type="STRING" id="656061.D5GH32"/>
    <dbReference type="GeneID" id="9185079"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007682001"/>
    <dbReference type="eggNOG" id="ENOG502SN7Z">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041923_0_0_1"/>
    <dbReference type="InParanoid" id="D5GH32"/>
    <dbReference type="OMA" id="NIGDFTH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024319">
        <property type="entry name" value="ATPase_expression_mit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47941">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12921">
        <property type="entry name" value="ATP13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ83825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="470" mass="53964" checksum="08BBDA16A4734590" modified="2010-06-15" version="1">MQAHNQGLTYQLSAGEFSSVLLSIRPRRYLSLRPRHYIDEYGRGQVHEVNYSFKEFRSRLRIVTQAMQQQGYQLGIIEYCHLLDCARAGGDLSMADDLWRQIVAREDLTPDTWTYNCYVAAKIGTIAIGRGVRMSQWTMQSRQTVTAKRMRALASQMYAQMIREAVHPNSMTFDLLVIAMSRAGDIGGVKELLMKVWGVNVNGLARSDNNLGSEPPTVSPDSVIYPTQHTLATFSMCFGANNDIETAIRVVDHISRRYKVPISLPAWINLLNWTYALTRPPAPVMPARSVLQTWETMRAPPYNVNPTIEMYDYLVRSLISRQYVSPAEDKMKEAVEMYVDLLERFIDLDRKYDTVVGETDLDTAVQQTEVLKDIAVVKRELHRFRSMMRRWVELLILGKGMNTKYTRRKVPDIVAAWAYFLGGKAIYTIDSGYIVLELGQEREWTPIPIRRRKRPWMPLGTGAKEVDYLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>D5GH44</accession>
    <name>D5GH44_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00007695001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat SWD2 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430297">
        <property type="protein sequence ID" value="CAZ83837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839646.1">
        <property type="nucleotide sequence ID" value="XM_002839600.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GH44"/>
    <dbReference type="FunCoup" id="D5GH44">
        <property type="interactions" value="997"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GH44"/>
    <dbReference type="GeneID" id="9185091"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007695001"/>
    <dbReference type="eggNOG" id="KOG1446">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044117_3_0_1"/>
    <dbReference type="InParanoid" id="D5GH44"/>
    <dbReference type="OMA" id="HNEGYIR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016070">
        <property type="term" value="P:RNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001194">
        <property type="entry name" value="Unplaced genomic scaffold supercont1.1, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037867">
        <property type="entry name" value="Swd2/WDR82"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19861:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19861">
        <property type="entry name" value="WD40 REPEAT PROTEIN SWD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 6" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="117"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ83837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="374" mass="41663" checksum="D652C58A4E18F381" modified="2010-06-15" version="1">MAAANPLHRQPELAKLSETITRYRPSKAFSNFAQGAEVTSLDFDDTGEFCIATGDDETLQLYDCKLGKHSKTLYSKKYGCHLARFTHLQSNVIYASTKENDTIRYLSLHDNSYIRYFKGHKRPVTSLEVSPVDDQFISCSNDDTVRVWDLRSHNCAGLLNITAPSLAAFDPSGIIFAVAAQSAGAILLYDLRNYDKQPFTTFSLTDDSFLSQYSYPPCMPAWSKLEFSNDGKHMLIGTRGHAHYVVDSFAADTAQFVYRAKRPNGPTGPKKLETSGDVCFTPDARFLIGGQGERGVVVWDSWVTPDEKKTLKPFIELAENTKSMASVVSFNPKMNLFATAHKDVVCCLFSLIPIFPFSLSLSRAIELSECIVLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5GH88</accession>
    <name>D5GH88_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00007797001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the THOC3 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430308">
        <property type="protein sequence ID" value="CAZ83913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839722.1">
        <property type="nucleotide sequence ID" value="XM_002839676.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GH88"/>
    <dbReference type="STRING" id="656061.D5GH88"/>
    <dbReference type="GeneID" id="9182618"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007797001"/>
    <dbReference type="eggNOG" id="KOG1407">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045202_1_0_1"/>
    <dbReference type="InParanoid" id="D5GH88"/>
    <dbReference type="OMA" id="WNADGRH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000445">
        <property type="term" value="C:THO complex part of transcription export complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040132">
        <property type="entry name" value="Tex1/THOC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22839:SF0">
        <property type="entry name" value="THO COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22839">
        <property type="entry name" value="THO COMPLEX SUBUNIT 3 THO3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="208"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00046343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ83913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="302" mass="33345" checksum="C421C985A1164FB2" modified="2010-06-15" version="1">MEPIVYLPVLTLRSIAPSYFTAAFHPSKSREYREHRPSSHSNNAVIRSIAWNNLGNRIACGLNTNLVRVWNPEKPEAKNSTELKGHLKDVEKVVWDPMHPDKLASCCNGQVKFWDYRINRCLGTTVTAGDNVNLAYHPDSKVLAVGTKDDRIHFIDPRNASAILKTHKEGSQTNQVTFSHNGDFVLITTSLGKVVLRHWPSLEEVYSVDAHHSAVNCLQLDPRGSTLAIGGSDAALSLWDTAHWVCLRTLTRMDAPIKSMGYSFDGAYVCASSDESTNIEIVCVLNCPQSNTLADGCFAGPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHC2</accession>
    <name>D5GHC2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00007744001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Gfo/Idh/MocA family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430308">
        <property type="protein sequence ID" value="CAZ83874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839683.1">
        <property type="nucleotide sequence ID" value="XM_002839637.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHC2"/>
    <dbReference type="FunCoup" id="D5GHC2">
        <property type="interactions" value="237"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GHC2"/>
    <dbReference type="GeneID" id="9182579"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007744001"/>
    <dbReference type="eggNOG" id="KOG2742">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023194_3_1_1"/>
    <dbReference type="InParanoid" id="D5GHC2"/>
    <dbReference type="OMA" id="IWVGMDE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006740">
        <property type="term" value="P:NADPH regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055170">
        <property type="entry name" value="GFO_IDH_MocA-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42840:SF5">
        <property type="entry name" value="NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42840">
        <property type="entry name" value="NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22725">
        <property type="entry name" value="GFO_IDH_MocA_C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase N-terminal" evidence="2">
        <location>
            <begin position="5"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="GFO/IDH/MocA-like oxidoreductase" evidence="3">
        <location>
            <begin position="147"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ83874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="348" mass="37849" checksum="95C288E599B1F28B" modified="2010-06-15" version="1">MAPIGIAILGCGIFVQEEHLPAVEACSDVVLKAVYSRSEASAKKLLQAKSGVDIYSDDSENGLGVLLARSDIQAVIIALPITIQPEVIKKALGAGKHVLSEKPVAKDLAQARELISYKQSYPDLNWSVAENFRFQEAWRHAHREITALGRVINFRSRVCWLVTPGWKYYETSWRKTPDYQGGFLLDAGVHYAASMRILLSDEKIVKVSAFTASNFEHLPPVDTISAIFKTDKGVVGSFEVSVGTTFEGWEFAVACENGSVTVRPVSEVVILRRKPGGGFEDEIVKKFPDDKKGVGPEVAAFAKAVIAGAPDERQTPELAVGDLEMIEKMLRSGENDGAVMEINGGLTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHX6</accession>
    <name>D5GHX6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00008174001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430322">
        <property type="protein sequence ID" value="CAZ84119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839928.1">
        <property type="nucleotide sequence ID" value="XM_002839882.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHX6"/>
    <dbReference type="GeneID" id="9181899"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008174001"/>
    <dbReference type="eggNOG" id="KOG0159">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022195_0_1_1"/>
    <dbReference type="InParanoid" id="D5GHX6"/>
    <dbReference type="OMA" id="MAIFHTI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11041">
        <property type="entry name" value="CYP503A1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46206">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46206:SF7">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0349">Heme</keyword>
    <keyword evidence="5 6 7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="16"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="6">
        <location>
            <position position="469"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR602403-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ84119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="532" mass="59761" checksum="0E44B75C677D66D6" modified="2010-06-15" version="1">MGIFIHLVDSGEVKNLYGYPIFGTTLALLSLGIFLLLFKEWSIHGPTNLPIVGVESPGYLARVKARRKFVSHGFQIVRDAYYKHYGKNFVITTNSNEKVILTYNQVKELSNAPDDTVSMSHSSAQTMMAQHTGLSGFVGIHYVREAVKAELNKNLGNMIGDLLEEAQFALRTELPGFATDEWVSVDIPAVALRAVARISGRAFIGLPLCRNEDWLALSIAFTTDVFQEVNKLTPVPKLLRPFFAYIWNSAGSVKSNKRKAQSLLAPVIQRRLEEEGLAEKNGTVRQNHRDLLQWLTARVTPDHKNVNVLSGMQLMTGLASIHTTGTGFTNMIFDLAEHQECIQPIREEMETLIAANGGVLDRATLRKMRKTDSFLRESFRTKLSLLGFNRMIMKNMTLSDGTYLPKGTLVAAPAAIFSSDPGFVEDPEVFDGFRWYKKSLGVADRAVDNNNASNTSPTNLIFSYGRHACPGRYFVVEVMKIMLAFMLLQYDIKYPEGQSRPPNIQMGEFSYPDRRQKILFRKRPGPKKFSFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GI30</accession>
    <name>D5GI30_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00008245001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430322">
        <property type="protein sequence ID" value="CAZ84173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839982.1">
        <property type="nucleotide sequence ID" value="XM_002839936.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GI30"/>
    <dbReference type="FunCoup" id="D5GI30">
        <property type="interactions" value="96"/>
    </dbReference>
    <dbReference type="GeneID" id="9183506"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008245001"/>
    <dbReference type="eggNOG" id="KOG3574">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020502_2_0_1"/>
    <dbReference type="InParanoid" id="D5GI30"/>
    <dbReference type="OMA" id="RRKSWIM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008521">
        <property type="term" value="F:acetyl-CoA transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035348">
        <property type="term" value="P:acetyl-CoA transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000289">
        <property type="entry name" value="Acetyl-coenzyme A transporter 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024371">
        <property type="entry name" value="AcetylCoA_trans_1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004752">
        <property type="entry name" value="AmpG_permease/AT-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12778:SF9">
        <property type="entry name" value="ACETYL-COENZYME A TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12778">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 33 ACETYL-COA TRANSPORTER -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13000">
        <property type="entry name" value="Acatn"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="145"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="213"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="250"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="286"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="324"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="438"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="472"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="507"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="577"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="79"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ84173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="620" mass="68522" checksum="5F7D7B7C06DD0D34" modified="2010-06-15" version="1">MSPAPLSESRDPLLPLTPSSSSTVRAHRSSSSAEHPDDFELENIYVDDLKTHCNSDDPATPSSPMARSRKDRHHDNDRSSSANMNSSSSVEYRRKNASVDGHDLLPSSMQTEALLQRESFSLDDDHDKQASASLKDLSPQDQKNFALLVLLYFLQGVPMGLAMGSVPFLLKSRLSYGEIGVFSLASYPYSMKLLWSPIVDAIWSKRMGRRKSWIVPIQALSAIMLLWLGANADRLMAEASGKLYTFTFVFFSLVMLCATQDVAVDGWALTLLSVENLSYASTAQTVGLTAGNFLSFTVFLAFNSPDFANRYFRTTPSDVGLFTLGGYLTFWGWAYLIVTLGLAVFKKEERTSNRDGLVSVYKTMWRVIKLKHVQTFIIIHLIAKIGFQANDAVTNLKLLEKGFTQEDMALTVLIDFPFEIGLGYYAGKWCAYFPPMTLWMWAFIGRLLAAVLAHITVWIFPSDGVVESGYLMVVIAEHIFSTFTSTVMFVAVSAFHAKIADPGIGGTYMTLLATVSNLGGTFPRIFVLKLVDAFTIATCTPLPDAKTPFEPFSCALEGDKKLCKTQGGVCNIEQDGYYITNVICIIIGVVTFWGYIKPAATRLSALPLKSWREASGPRSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>D5GID7</accession>
    <name>D5GID7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008438001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430326">
        <property type="protein sequence ID" value="CAZ84280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840089.1">
        <property type="nucleotide sequence ID" value="XM_002840043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GID7"/>
    <dbReference type="GeneID" id="9184638"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008438001"/>
    <dbReference type="HOGENOM" id="CLU_1579646_0_0_1"/>
    <dbReference type="InParanoid" id="D5GID7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="169" mass="18296" checksum="7DCBFCF86CE24A7D" modified="2010-06-15" version="1">MSGTTSFSGAQTSYIDKSLVLSTPPDSESEDTYATRSKSQPTRRIPDPTPTGSYWSHQQSNSEKPAGPQPESSATINLHLGDDNDGCGIMQNSFNTTITYSGEKISEIERVMGSGPQFKSQPTIVASAGKRNKNSIQFRDVGATRIIINDGTKVEDAHQRQKRKGPDSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIM7</accession>
    <name>D5GIM7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00008570001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EMBL" id="FN430327">
        <property type="protein sequence ID" value="CAZ84370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840179.1">
        <property type="nucleotide sequence ID" value="XM_002840133.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIM7"/>
    <dbReference type="STRING" id="656061.D5GIM7"/>
    <dbReference type="GeneID" id="9183304"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008570001"/>
    <dbReference type="eggNOG" id="ENOG502S1WE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_662558_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIM7"/>
    <dbReference type="OMA" id="HPCWFIP"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045048">
        <property type="entry name" value="FBXO31/39"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10706">
        <property type="entry name" value="F-BOX FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10706:SF130">
        <property type="entry name" value="F-BOX ONLY PROTEIN 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12014">
        <property type="entry name" value="Cyclin_D1_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="F-box" evidence="3">
        <location>
            <begin position="10"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ84370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="415" mass="46521" checksum="C36B687A3D8CEC92" modified="2010-06-15" version="1">MASSDEGANSTGIKSCPNEVLQLVFQNLNGVSLARCSVVCSHWRELIDPSDPATPDYLWARQASSLRLKSPTPYTRFRDLYASLRDHLWLNGKIWMSNRLWTGYILLSTYNLETGAIDLTNVVAARPGGSGREDSAPWSRNESIFVSSFDPQVSLWTLPLLSFDKDTVLDPNDNEIKPPTQERYFSCSLFRASAIPPEVQVSSKKYWPPPHIPATERTSDVLSCINGPHESNLFHGDAIDSAFRIRRWVVFGGAPVPSVAVSRSGVDNQVETFATIREEHYTPDKDHPYRGIWVGNYSSHGYEFILLHQETSTKLKAVKITGDLNVPRGEITFVVDDLKTVIRIANEREWPGAKVISARAQIAFHQFQSSKMIDAEMICVGPDDIALLWHFSPLPSHISRFKRVDIEGLIYGCGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJL1</accession>
    <name>D5GJL1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00009062001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430332">
        <property type="protein sequence ID" value="CAZ84704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840513.1">
        <property type="nucleotide sequence ID" value="XM_002840467.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJL1"/>
    <dbReference type="GeneID" id="9186738"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009062001"/>
    <dbReference type="HOGENOM" id="CLU_1448719_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJL1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ84704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="187" mass="21479" checksum="53A83C50C5A380AF" modified="2010-06-15" version="1">MHDLRSPNETVARWGSFICKPIRSLHTLDSGKFLVGHSEACMLRVFDIRSKYCDLGERIPIGGEWSKYPYSWVTNFQLPGRSRPEPMDPVLALAVSPWTEKSVYAATPGMVWELNFAGTMTRAPEHVPVAPGVPAWKKMWKRSRVWRKADGEKEMVEPVTLAVKAYNGLLWGMHHQQPPRRVYQRYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJN6</accession>
    <name>D5GJN6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="16">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">GSTUM_00009088001</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the XPG/RAD2 endonuclease family. EXO1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430332">
        <property type="protein sequence ID" value="CAZ84729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840538.1">
        <property type="nucleotide sequence ID" value="XM_002840492.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJN6"/>
    <dbReference type="FunCoup" id="D5GJN6">
        <property type="interactions" value="197"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJN6"/>
    <dbReference type="GeneID" id="9186763"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009088001"/>
    <dbReference type="eggNOG" id="KOG2518">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008978_3_0_1"/>
    <dbReference type="InParanoid" id="D5GJN6"/>
    <dbReference type="OMA" id="DVQFRAM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035312">
        <property type="term" value="F:5'-3' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
        <property type="term" value="F:5'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09908">
        <property type="entry name" value="H3TH_EXO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09857">
        <property type="entry name" value="PIN_EXO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000011">
        <property type="entry name" value="exonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000002">
        <property type="entry name" value="Exonuclease 1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037315">
        <property type="entry name" value="EXO1_H3TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044752">
        <property type="entry name" value="PIN-like_EXO1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006086">
        <property type="entry name" value="XPG-I_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006084">
        <property type="entry name" value="XPG/Rad2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019974">
        <property type="entry name" value="XPG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006085">
        <property type="entry name" value="XPG_DNA_repair_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081:SF65">
        <property type="entry name" value="DNA DAMAGE-INDUCIBLE PROTEIN DIN7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081">
        <property type="entry name" value="FLAP ENDONUCLEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00867">
        <property type="entry name" value="XPG_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00752">
        <property type="entry name" value="XPG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00853">
        <property type="entry name" value="XPGRADSUPER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00484">
        <property type="entry name" value="XPGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00485">
        <property type="entry name" value="XPGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00842">
        <property type="entry name" value="XPG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0267">Excision nuclease</keyword>
    <keyword evidence="8" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XPG N-terminal" evidence="15">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="XPG-I" evidence="14">
        <location>
            <begin position="138"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAZ84729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="374" mass="42588" checksum="E3CB377BCD9671E7" modified="2010-06-15" version="1">MGISGLLVRLGSIQRKNHIKEWNGTTVGIDAYSWLHKGVIGCAIELAQGKHTRGYVEYAMSRVHMLRHFGVTPYMVFDGDYLPSKSWTEAERERRRQERRAKGLELLKLRKMREAQEQLQKSIDVTPLMARHLIDECRRIGVECIVAPYEADAQLYYLEKTGIIHGIISEDSDLLAFGCKNLITKMDKFGGCYTIDRDDFTAIRGSGFSLSGWTDTEFRYMCILSGCDYLPNIPRLGLLGAYKLVKRHKTPERIIQSVRFDATKTVPVDYLDGFRRANLTFLHQWVFCPKAEKMVMVTEPDAVVDKQYLDGCGRELDAETARGIACGDLDPVTKKPFVLESIVESAIRSAWMKPKMRVLETSQSFTSISNFFSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJV4</accession>
    <name>D5GJV4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00009215001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430334">
        <property type="protein sequence ID" value="CAZ84797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840606.1">
        <property type="nucleotide sequence ID" value="XM_002840560.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJV4"/>
    <dbReference type="STRING" id="656061.D5GJV4"/>
    <dbReference type="GeneID" id="9186833"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009215001"/>
    <dbReference type="eggNOG" id="ENOG502S9Q7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019744_2_0_1"/>
    <dbReference type="InParanoid" id="D5GJV4"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ84797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="365" mass="40799" checksum="F704539356465BBF" modified="2010-06-15" version="1">MEDIELYKLDVAGFKKDIARRDALLAEQAHALKSHPEEIEALRASHSRLLAEISRCRTEANGIVAERDKEIQRLEKVVGGLKHSISTNTRIEEAVGDEVFREMWSRIGWEVMNWCLANGAAKGGVINVESLKDDVRKELQTGIPEYEKLILGGHRVHVVQAFLARLLIREIFSDWCFGITKEQNSVLSALEKEFTRSSPQPAINTWRSSTSILLRTSLLTPPTATLTNKIISVTSTIFSAITPFFPTPPPQTSKTLPGIVEQTIRLSLHMQTQRAQFRIFPPLSFSSSTAGAQDQGGEDERGVEGEMIWNNEDMEELSGEDESELEGAKVLVVAWPGMEKWGDEMGECCHIRNCVVRARVVCDVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5GK86</accession>
    <name>D5GK86_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00009424001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430337">
        <property type="protein sequence ID" value="CAZ84929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840738.1">
        <property type="nucleotide sequence ID" value="XM_002840692.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GK86"/>
    <dbReference type="STRING" id="656061.D5GK86"/>
    <dbReference type="GeneID" id="9184858"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009424001"/>
    <dbReference type="eggNOG" id="KOG1817">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_519905_0_0_1"/>
    <dbReference type="InParanoid" id="D5GK86"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00593">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="entry name" value="Ribonuclease III domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11207:SF0">
        <property type="entry name" value="RIBONUCLEASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11207">
        <property type="entry name" value="RIBONUCLEASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00636">
        <property type="entry name" value="Ribonuclease_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00535">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="RNase III domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00517">
        <property type="entry name" value="RNASE_3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50142">
        <property type="entry name" value="RNASE_3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RNase III" evidence="3">
        <location>
            <begin position="274"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="49"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="206"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="109"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="127"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="206"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="218"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="238"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ84929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="524" mass="58742" checksum="7EB6FB23BCCDAFFD" modified="2010-06-15" version="1">MIPFVIKNFSYDVWSLDWINIQWVGLGPFFLVLIGLSYDLLPPYQQPPHSKLLEASPQNASIKMVRSRKRRHDDTDAITVSSPSRKESKRSKGCPDYLTWGIPKTKGLPQDEKRRQEQRHEADESGRAQSSIFSSDVCSARKPPALDISQKFDAFGSAFAELILAAPNVNMEKCLGDEKANFFTAVRMMFETGDLPWLDEYMKKAKEARGNSEKSKKTPPANGNNPTGPLKNAVPKASDSSPSTYTPPSTTDPALLSPSFSSWPPPLPEIHSDELRQQAFTHKSFIKGEDAGLPGIEQQHYERLEFLGDSYLQSITSRILYNRFSKLREGPLSDMRQQLVANRPLSTYATIYRFHDKVRESKGQNTAAPVALVHNSRGTYKDMGVNKTLADCFEAYIAAIVLDSPNGVDIAQRWLTELFEPKLKEMETQHASVGPVDKMAKQTLNTIVGGNRATVEYVWTDGEGGNKGGYWITVYLTGWGFNKREIGSGWGNNKGYYISLPSFFLPIIHTRVESIHLANVLRKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKF1</accession>
    <name>D5GKF1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00009508001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="FN430338">
        <property type="protein sequence ID" value="CAZ84994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840803.1">
        <property type="nucleotide sequence ID" value="XM_002840757.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKF1"/>
    <dbReference type="FunCoup" id="D5GKF1">
        <property type="interactions" value="819"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GKF1"/>
    <dbReference type="GeneID" id="9182014"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009508001"/>
    <dbReference type="eggNOG" id="KOG1870">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001060_9_0_1"/>
    <dbReference type="InParanoid" id="D5GKF1"/>
    <dbReference type="OMA" id="KPRGCTG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02674">
        <property type="entry name" value="Peptidase_C19R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2230.10">
        <property type="entry name" value="DUSP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035927">
        <property type="entry name" value="DUSP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006615">
        <property type="entry name" value="Pept_C19_DUSP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028135">
        <property type="entry name" value="Ub_USP-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF24">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06337">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14836">
        <property type="entry name" value="Ubiquitin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00695">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143791">
        <property type="entry name" value="DUSP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51283">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="DUSP" evidence="10">
        <location>
            <begin position="69"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="9">
        <location>
            <begin position="388"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="341"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1006"/>
            <end position="1104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1148"/>
            <end position="1176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="52"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1016"/>
            <end position="1032"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ84994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1176" mass="128875" checksum="89A9DAF5377B27AC" modified="2010-06-15" version="1">MPSAEEKSRREPSVEVADGSVASSEGNGGVIHIDATSTAVSIAPSTATTIGSTQPSSASATPVSSVSIPSLEDQAHFIIKSKIETKPKEGDLGYIISGPWLNQLLAKFSDSGVQLTKEGAEGEIGLIDNSDLVDTTQMQGQAKKTSDDSTVEDSAVPEDFVAIKPGILGDDFEILPEEAWNSLVSWYGLAEGSPVITRRAVNTSETNVPHIQYEIYPPTFTFYKLRDPSTNITKDVLDKEKSQSPKRITAGKTDGFQKLLRKVKKLAGVDAARKIRLWRIIESSFEHDLEPASKSSRKAGLGAGGTIVIEEQGSDGEWISEKPTKSVKKGGQQVTVALNGKNITGGPVKKKASARPDSPSGSTSTAVIRSGFLNRPAERRDGRPLGKCGLSNLGNTCYMNSALQCLRSVTELSNYFLCNKYMEELNPSNPLSHSGKVAKAYATLLGYIFSPTCPVSVSPREFKSTISRFSLSFSGYGQQDTQEFLAFLLDGLHEDLNRIQKKPYIEKPESTDKMVGDGEAIARLAQEHWTIYKRRNDSVIADLFGGLYQSTLVCPDCEKVSITFDPFMDLTLPLPVENVWGRDIYFYPSSASGGGLIKIPVEMDKNSSIKSLKEHLARKFNLDPKKTMASEVYKGKFFKHYEDFQTVSETIRQDDDAFIYELDDVPTNYPPSKSKKPRRTGFYGPSYVFGLPFFIVVNRDEVRSFDAIVKKIIAKYQVLTTRNFYESEASPSESETEEVVEVKDELEDINETEEETHDGFVDVSMKDASSMVGSDRKAVKSPRRPLPAGIEDLFTVMATKRRSGDSAVVTGWQALETAFDIRERLNHVQSASPPRRKTSDYFTNERKRKGIHLEDCLDEFAKEEVLSEEDPWFCPRCKVHRRASKKFELWKCPDILVIHLKRFSSSRNFRDKIDVLIDCPVTGLDLQERVGLKEEGKSLVYDLIAVDNHYGGLGGGHYTACAKSWIDGKWYHYDDSSVKEVGEQKVITSAAYLLFYRRRSETTLGGPRLNWMLSEPTDSPGSASSSRNTSPTRAGEGGPSGDSSATVLPNGRSSGGGTSGLSTIYSTPGNSNLWGNEPPPSYSESSGMEMILMPGTQGSPTSSKAPTVGFSFGSRGGLLRDSTDDGVFIGSLLGPAEHTDIPTDITVDDDEGLLPTLSDGNNDTAGVHDVTDPMEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKT6</accession>
    <name>D5GKT6_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed RNA polymerase</fullName>
            <ecNumber evidence="2">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">GSTUM_00009762001</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="2"/>
    <dbReference type="EMBL" id="FN430341">
        <property type="protein sequence ID" value="CAZ85129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840938.1">
        <property type="nucleotide sequence ID" value="XM_002840892.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKT6"/>
    <dbReference type="FunCoup" id="D5GKT6">
        <property type="interactions" value="1048"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GKT6"/>
    <dbReference type="GeneID" id="9183365"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009762001"/>
    <dbReference type="eggNOG" id="KOG0260">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000487_1_1_1"/>
    <dbReference type="InParanoid" id="D5GKT6"/>
    <dbReference type="OMA" id="HESFEWL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140463">
        <property type="term" value="F:chromatin-protein adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003968">
        <property type="term" value="F:RNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0180034">
        <property type="term" value="P:co-transcriptional lncRNA 3' end processing, cleavage and polyadenylation pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:intracellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019985">
        <property type="term" value="P:translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02584">
        <property type="entry name" value="RNAP_II_Rpb1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.30:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.390:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.274.100:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.140">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.100">
        <property type="entry name" value="RNA polymerase Rpb1, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045867">
        <property type="entry name" value="DNA-dir_RpoC_beta_prime"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000684">
        <property type="entry name" value="RNA_pol_II_repeat_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007066">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042102">
        <property type="entry name" value="RNA_pol_Rpb1_3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007083">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007081">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007075">
        <property type="entry name" value="RNA_pol_Rpb1_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007073">
        <property type="entry name" value="RNA_pol_Rpb1_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038593">
        <property type="entry name" value="RNA_pol_Rpb1_7_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038120">
        <property type="entry name" value="Rpb1_funnel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376:SF37">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04983">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05000">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04998">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04992">
        <property type="entry name" value="RNA_pol_Rpb1_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04990">
        <property type="entry name" value="RNA_pol_Rpb1_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05001">
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        <property type="match status" value="8"/>
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        <property type="entry name" value="RNA_POL_II_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
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    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
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        <location>
            <begin position="24"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb1" evidence="20">
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            <begin position="208"/>
            <end position="313"/>
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            <begin position="392"/>
            <end position="438"/>
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    <feature type="domain" description="RNA polymerase Rpb1" evidence="17">
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            <begin position="529"/>
            <end position="558"/>
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    </feature>
    <feature type="domain" description="RNA polymerase Rpb1" evidence="17">
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            <begin position="560"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="792"/>
            <end position="816"/>
        </location>
    </feature>
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            <begin position="832"/>
            <end position="1025"/>
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            <end position="216"/>
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            <end position="895"/>
        </location>
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            <begin position="905"/>
            <end position="951"/>
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            <begin position="962"/>
            <end position="993"/>
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    </feature>
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            <begin position="994"/>
            <end position="1004"/>
        </location>
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        <location>
            <begin position="1005"/>
            <end position="1017"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012418"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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            <dbReference type="SAM" id="Coils"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="Pfam" id="PF05000"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAZ85129.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
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    </evidence>
    <sequence length="1025" mass="111052" checksum="E4428ECF3D0B2B2B" modified="2010-06-15" version="1">MNLHVPQSEETRAEVSQLCMVPLNVVSPQKNGPLMGIVQDTLCGIYKMCRRDVFLNREQVMNILMWVPNWDGTIPTPAILKPRPRWTGKQMISLVIPPGINKVDLAGGKWSPLNDEGLLVHGGELMFGLLNKKAVGQSHLSLIHIIYNEKGAYRCLEFFNGCQTIVNYWLLHNGFSIGIGDTIPDKLTIEKIAATVKEKKDEVNAVTDKAQRNELEPLPGMNIRDTFENLVQKSLNSARDKAGSDTEKSLKDSNNAIQMARSGSKGSNINISQMTAVVGQQSVEGKRIPFGFKYRTLPHFTKDDYSPESRGFVENSYLRGLTPQEFFFHAMAGREGLIDTAVKTAETGYIQRRLVKALEDVMAKYDGTVRNSLGDIIQFIYGEDGLDGIVDEKLQLPINILRILENARTIFRIKHRARSNLHPLEVTEKVKELVKKLVSRFARAAVQPGEMVGVLAAQSIGEPATQMTLNTFHFAGVSSKNVTLGVPRLKEILNVAKNIKTPSMMVYQVPENTHEKETAKALRSAVEHTTLRSVTETTEIWYDPDPRDTVIEEDKETVFDDNAEIPNIRCRMKNADQAKSLDEESDLEEDTMLKRLENHILDELTLNGVLEVEGVDTTRTYSNTFEEILQVFGIEATRGALLRELRQVLAFDGSYVNERHLALLCDVMTSRGHLMAITRHGINRADTGALMRCSFEETVEILLEAAASGELDDCRGISENVMLGQLAPLGTGEFDVFLDEKMLAEVIVDNSRVTGGEIPGMGKGVLADGAATPYDSSSPMYGDPYVGSPDAGAAFSPMQHTGSETPGGLTDYQAPGFGSSYGGAGARSPLTYSPTSPFGTSPTSPGYSPASPAYSPTSPNMAITSPGFSAASPRFSPASPAYSPTSPGYSPTSPAYGGGLGVGGSPTSPNYSPTSPSFSPTSPTYSPTSPNYSPTSPAYSPTSPDYSPTSPAFSGGVGNSHSPTSPTSPNYSPTSPVYSPGSPAASYSPTSPTFQSPTQGSPRWSPTSPGPSYSPTSPRDPPRNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKU8</accession>
    <name>D5GKU8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="16">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">GSTUM_00009777001</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family. SWR1 subfamily.</text>
    </comment>
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        <property type="protein sequence ID" value="CAZ85141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002840904.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="9183377"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009777001"/>
    <dbReference type="eggNOG" id="KOG0391">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000315_24_2_1"/>
    <dbReference type="InParanoid" id="D5GKU8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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    <dbReference type="GO" id="GO:0000812">
        <property type="term" value="C:Swr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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    <dbReference type="GO" id="GO:0140849">
        <property type="term" value="F:ATP-dependent H2AZ histone chaperone activity"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031492">
        <property type="term" value="F:nucleosomal DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="P:recombinational repair"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045815">
        <property type="term" value="P:transcription initiation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050520">
        <property type="entry name" value="INO80/SWR1_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45685:SF1">
        <property type="entry name" value="HELICASE SRCAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45685">
        <property type="entry name" value="HELICASE SRCAP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0010">Activator</keyword>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="448"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="724"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="49"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="159"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="382"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="937"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="49"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="64"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="97"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="134"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="174"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="213"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="301"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="312"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="388"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="407"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="970"/>
            <end position="980"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAZ85141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1046" mass="118773" checksum="BFE9D9AA6BA22238" modified="2010-06-15" version="1">MAEIKRRRPLTKEEIEQEEFFENRRIYREQISQLRRKWEEVLKEVENRRRMRAEEEHRLAGKQHLNQMLEHSTQLLEARRGDRHNSMTASEGGLDMAEGEYDGEEGGEEEEEDDSKSMSELEVELDEANMSTTDSDEEEVEDDDESLTVEQLKAKYAGLGPSQASLGGKRLIQEDDLSDIEDGMLLDGRTPVPSDVPSDDENPEDVAARMDVDENQTEAVELEEVDEILMDDSDESVDMDSEDETDDSESGKDGDSEDDSEGENGFGSGLLGLYGDLNGLRASSRDLEPESEDENEFTEPVQKDRSLERSSDSMQIESLSKSSKINGSKLNAADELLEEDRIDEVSLVVYKSPPGKTVGDSYVSAPRVIGGQALEIPMLNGHSDTMDVETSTSLSHSQIDLKESGHPSSLTTSPETSPQPNIDIKTPIPFLLRGTLREYQHYGLDWLAGLYNNHTNGILADEMGLGKTIQTIALLAHLACEKEVWGPHLIVVPTSVMLNWEMEKGWFDNNLWHVCITSYQLVLQDQVTFKRRNWHYLILDEAHNIKNFRSQRWQTLLNFKAEARLLITGTPLQNNLIELWSLLYFLMPSGASSAMPAGFANLKEFQEWFAHPLHRVLRPYLLRRLKADVEKQMPAKFEHIVYCRLSKRQRYLYEEFMSMAKTRETLASGNYLSIINCLMQLRKVCNHPDLFETRQIVTSFAMQKSVVADYEVKELLYDCGKLQRLDLLLRQLQAGGHRALIFTQMTKVLDILEQFLNIHGHRYLRLDGATKVEQRQILTDRFNNDTRILVFILSTRSGGLGINLTGADTVIFYDLDWNPAMDKQCQDRCHRIGQTRDVHIYRFVSEYTIESNILRKSNQKRMLDDVVIQEGDFTTDYFNKLTIRDMLGDEVIKDLDGVDDSQTVVAPGVNMEKALEAAEDVEDVAAAKVAQREVVDADGADFAERSTPAQTPREPGTATAGTTPREPSAPVSNAAMTPQGNTVELEVVTHRGLGYDIYGDGWNRVDREPVHIDEYMIRYMEWELVDVPLVLPSKSKKKKSKKGKKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5GL87</accession>
    <name>D5GL87_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00010064001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430347">
        <property type="protein sequence ID" value="CAZ85280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841089.1">
        <property type="nucleotide sequence ID" value="XM_002841043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GL87"/>
    <dbReference type="STRING" id="656061.D5GL87"/>
    <dbReference type="GeneID" id="9182091"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010064001"/>
    <dbReference type="HOGENOM" id="CLU_2724051_0_0_1"/>
    <dbReference type="InParanoid" id="D5GL87"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ85280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="72" mass="7901" checksum="4A01D140C79A6FA4" modified="2010-06-15" version="1">MAAQIHKAFETRTCEFLTFSGLAPTVSAKILENLLSLKDPIALGLRINFIAPDGYLRIVMASRLHETAVALM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLG2</accession>
    <name>D5GLG2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00010180001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430348">
        <property type="protein sequence ID" value="CAZ85355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841164.1">
        <property type="nucleotide sequence ID" value="XM_002841118.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLG2"/>
    <dbReference type="GeneID" id="9186099"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010180001"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097643_0_0_1"/>
    <dbReference type="InParanoid" id="D5GLG2"/>
    <dbReference type="OMA" id="DECNMAS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ85355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="157" mass="17271" checksum="822AB3308670D6CC" modified="2010-06-15" version="1">MSDTLGASSSLTTACRELPIQSALCTFDCAQVLAEWIATVQERVNPYMGVLGSDECNMASLEGLVMLEHEDRKLLAKVMEMLRNAEMKMSMDWNNNPNSQGNNILRADSGLGSKVLNVSAYLLDKVVIWPATRLMAQALRAQSEHMEARVKNGGLTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLJ5</accession>
    <name>D5GLJ5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00010237001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the saccharopine dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430349">
        <property type="protein sequence ID" value="CAZ85388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841197.1">
        <property type="nucleotide sequence ID" value="XM_002841151.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLJ5"/>
    <dbReference type="GeneID" id="9186132"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010237001"/>
    <dbReference type="eggNOG" id="KOG2733">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031002_0_1_1"/>
    <dbReference type="InParanoid" id="D5GLJ5"/>
    <dbReference type="OMA" id="GPYQLYG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009247">
        <property type="term" value="P:glycolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051276">
        <property type="entry name" value="Saccharopine_DH-like_oxidrdct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005097">
        <property type="entry name" value="Sacchrp_dh_NADP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12286">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE-LIKE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12286:SF5">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE-LIKE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03435">
        <property type="entry name" value="Sacchrp_dh_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="278"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="Saccharopine dehydrogenase NADP binding" evidence="3">
        <location>
            <begin position="13"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ85388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="409" mass="46052" checksum="2E540A8797216DB6" modified="2010-06-15" version="1">MALPHVQRKYDLIVFGASGYTGRLTAEQVLQHTPSDLKWAIAGRSPHKLELLATDFNRRFPDRVPVGIFIADLDEDALEKMARATRCLVSTVGPFIRYGTAVVEACAVNGTHYVDSTGEITWVKEIVDKYHKTAKANGAIMIPQCGMESAPADLAAHTLVKIMRDSYNCPVGDVTFCLHELKAGMSGGTLESGMSLLEHSTLQEVVHATRTYALSPVRGYDLPFAWPMRRHPELGILTKWIQAVPDTVYVYRSWGTFDRGQYYGENFNFMEYRRASNWISAVFWFLMMCSITVLPIFAPFRWLARRLIQPGEGPTAEQMEKYRMEYRGVAEADDDSEDPKKVFVRLTYEKDPYTCTGALMVQVAMSILFDEGIEARKLGCGMLTTATVATPEFYRNLDRAGFCIETKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLR7</accession>
    <name>D5GLR7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00010372001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sideroflexin family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430351">
        <property type="protein sequence ID" value="CAZ85484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841293.1">
        <property type="nucleotide sequence ID" value="XM_002841247.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLR7"/>
    <dbReference type="FunCoup" id="D5GLR7">
        <property type="interactions" value="266"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GLR7"/>
    <dbReference type="GeneID" id="9186468"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010372001"/>
    <dbReference type="eggNOG" id="KOG3767">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039425_0_0_1"/>
    <dbReference type="InParanoid" id="D5GLR7"/>
    <dbReference type="OMA" id="GRVRHCA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015194">
        <property type="term" value="F:L-serine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015075">
        <property type="term" value="F:monoatomic ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071454">
        <property type="term" value="P:cellular response to anoxia"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140300">
        <property type="term" value="P:serine import into mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004686">
        <property type="entry name" value="Mtc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11153">
        <property type="entry name" value="SIDEROFLEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11153:SF6">
        <property type="entry name" value="SIDEROFLEXIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03820">
        <property type="entry name" value="SFXNs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="146"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="259"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ85484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="314" mass="33755" checksum="A78C2C0868115359" modified="2010-06-15" version="1">MSSSILGTRELPLSQYDLSTYWGRVRHSADIADPRTLFTSQKSLDAHTALLAKYKRGEIPPQMTPELWHAKKVVDATLHPDTGAPVFLPFRMSCFVFSNLVVTAGMLAPNLTTRGVLGWQIANQTLNVLINTSNANKSSPLSNGQLAASFLLAVSASCGVAVGLGRVVQKVKFRPNVKTILGRLVPFAAVATAGVMNVCLMRGGEIEKGIDIYPASDGGEKKESLGKSRKAAIFAVGETAVSRVFNATPIMVIPPLLLLIMPINCGLIFATSIFALPLALGAFPQRQAVKAESLEPYFWGKGGREDLVEFNRGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>D5GM08</accession>
    <name>D5GM08_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00010349001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="FN430351">
        <property type="protein sequence ID" value="CAZ85470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841279.1">
        <property type="nucleotide sequence ID" value="XM_002841233.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GM08"/>
    <dbReference type="FunCoup" id="D5GM08">
        <property type="interactions" value="287"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GM08"/>
    <dbReference type="GeneID" id="9182773"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010349001"/>
    <dbReference type="eggNOG" id="KOG0583">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003954_1_1_1"/>
    <dbReference type="InParanoid" id="D5GM08"/>
    <dbReference type="OMA" id="YVEPRSQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045033">
        <property type="term" value="P:peroxisome inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000011">
        <property type="term" value="P:vacuole inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14076">
        <property type="entry name" value="STKc_Kin4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000397">
        <property type="entry name" value="Serine/threonine-protein kinase KIN4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034674">
        <property type="entry name" value="STK_Kin4/ppk1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF110">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="230"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="518"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="976"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="28"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="64"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="112"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="147"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="539"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="654"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="675"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="713"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="767"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="837"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="864"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="885"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="916"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="953"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ85470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1027" mass="109124" checksum="92C0F158865007ED" modified="2010-06-15" version="1">MSSAIASQSVPAPSNPSPPRAGRPSHPRPTTYTGLSPTVHTTVESSPVNPSQLSHGYSGSVSPGGPPIPPPPRTSSQRQGPAVTSPPAASQVPASTERSKSSSGRTRGQSTDGREREKTDRDRERDRRRAEENKPRRDQPPSAATVGRSNSTRDSSRNVAGSSSGSQQPVDRSPSTRRRQDAVDGAGASTSGQSRSRREQSGNGGLAVVNPDGNGTRPAGTRKETRFGEYILGQTLGEGEFGKVKLGWRRDGGVQVAIKLIRRDSLSPHPNRLSKIHREISILRTLSHPNIVRLHEMVETDRHIGIILEYASGGELFDYILNHRFLKDPPACRLFAQLVSGVGYLHKKGIVHRDLKLENLLLDRNRNIIITDFGFANVFDPRDELGDLMATSCGSPCYAAPELVVSDGLYTGRKVDVWSCGVILYAMLAGYLPFDDDPANPEGDNINLLYKYIVSTPLTFPEYVSPHARDLLRRMLVPEPRKRADLFEVARHSWLSPFANVVSLITSSTTGVKEVEHVTINEPTLDGPSLVRSASVREPSSTNSHPHPTSLGGLGPRHHAHENVPQAAQSKPVSDAKRRTVQVEYVEPRSQTKRGSAADPDQGASSEDAAPAPFVPTNKHNDGVTRAATTAGIPSRHQRTMSSDQQQKPRDQLAQPSGSSAQPPRTRPSSSHPQAPARAATTAGDGAGSGPPSRRPSRDRPGPQFNGKVFTPAEQTSSNSARPNTANSINSNRLPSRGIYSRPVAPSLAANNAQGRIAMPKAPKPYTISNPIPQPDNSDAIGAPASAGEMGKPSTTVLPTKYSGRPSPPAEQSAKGHKRANTIGSGGASRFLGGLMGSSSSQDNTSRPTMTGAGEPRPSVDQGGRSKYEKTKEGKVRRFSLLPTSFSLRSISGSGSEGKHERRPSRSASGLRVGQFINSKSQPQTSSHPQLSAPNAGEGGSVRTGSDTSLAVGGSAPASAAGGNYARESIDLGAGNANAARRPAVLQKNRKFTEGDAGGTGGSSGPARRVMDFFRRRGVARSSKGDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>D5GM26</accession>
    <name>D5GM26_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00010495001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430351">
        <property type="protein sequence ID" value="CAZ85569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841378.1">
        <property type="nucleotide sequence ID" value="XM_002841332.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GM26"/>
    <dbReference type="FunCoup" id="D5GM26">
        <property type="interactions" value="211"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GM26"/>
    <dbReference type="GeneID" id="9187353"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010495001"/>
    <dbReference type="eggNOG" id="KOG1547">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017718_7_1_1"/>
    <dbReference type="InParanoid" id="D5GM26"/>
    <dbReference type="OMA" id="QCEFVYL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032156">
        <property type="term" value="C:septin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006698">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="5">
        <location>
            <begin position="29"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="292"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="309"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ85569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="320" mass="36343" checksum="7B291BA92057335A" modified="2010-06-15" version="1">MSQAATVFPRSHVGFDSITSQIERKLLKRGFQFNVICVGQTGLGKSTLINTIFAAHLIDSKGRLTPDEPVRSTTEIQTVSHIIEENGVRLRLNIVDTPGYGDQVNNDRCWDPIVKYIKDQHSAYLRKELTAQRERYIQDTRIHCCLFFIQPSGHALKPIDIVVLKKLSDVVNVVPVIAKEEFTFHNLKMYPYDNDELDEEERALNAQIKNIIPFAVVGSEKSIIVDGKQVRGRQNRWGVINVENENHCEFVFLRNFLTRTHLQDLIETTSQIHYETFRAKQLLALKESSAAGAGSSRPISPAAERELSRGSQHRMTINGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMC7</accession>
    <name>D5GMC7_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">serine C-palmitoyltransferase</fullName>
            <ecNumber evidence="5">2.3.1.50</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00010652001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.50" evidence="5"/>
    <dbReference type="EMBL" id="FN430354">
        <property type="protein sequence ID" value="CAZ85670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841479.1">
        <property type="nucleotide sequence ID" value="XM_002841433.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMC7"/>
    <dbReference type="FunCoup" id="D5GMC7">
        <property type="interactions" value="851"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GMC7"/>
    <dbReference type="GeneID" id="9183240"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010652001"/>
    <dbReference type="eggNOG" id="KOG1358">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015846_0_2_1"/>
    <dbReference type="InParanoid" id="D5GMC7"/>
    <dbReference type="OMA" id="ITIRHAI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004758">
        <property type="term" value="F:serine C-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046512">
        <property type="term" value="P:sphingosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000059">
        <property type="entry name" value="Serine palmitoyl CoA transferase subunit LcbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050087">
        <property type="entry name" value="AON_synthase_class-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693">
        <property type="entry name" value="CLASS II AMINOTRANSFERASE/8-AMINO-7-OXONONANOATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693:SF2">
        <property type="entry name" value="SERINE PALMITOYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="11">
        <location>
            <begin position="134"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ85670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="506" mass="56541" checksum="42E04FE3F477D794" modified="2010-06-15" version="1">MDLHDAYVLLNWSAGELSTRFQRVPGSQILLRYIKSSYQNDPVRSVVELCLFLFAVRYLLAPRYSTTQKGAVELTEDEIDELVEDWTPEPLVPGLTEVEQAENEKRPVIVGPTGPKVKLSNGRTVNNFASYNFFNFVSNELLKDRAVQVLRNYGVGACGPPNFYGTQDVHMDTERDIAKFLGMPACIIYAQAFSTISSVIPAFSKRGDIIVADKAVNFAIQKGLQISRSTIRWYEHNDMEDLGRVLEQVKRDMARKPLTRRFIVTEGLFENTGDIVDLPAIIELKQRYKYRLILDESYSFGVLGRTGRGVTEHQNVDPQSVEMLVGSLAHGICSGGGFCAGSPEVVDHQRISSSAYVFSAALPAMLAVTTSETLKLLEGNPQVLTALRENIKAFRNQMDRCEYVECTSSLDNPVCMFILKRDGLEGRIFGNEEKILMDIVDEALANSVMISRVKHIYETQGTKPFGWRCVPSFKACITIGHTKKEVEKAGSVVRQAVGKVMARLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMH3</accession>
    <name>D5GMH3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00010729001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430356">
        <property type="protein sequence ID" value="CAZ85716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841525.1">
        <property type="nucleotide sequence ID" value="XM_002841479.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMH3"/>
    <dbReference type="GeneID" id="9182171"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010729001"/>
    <dbReference type="HOGENOM" id="CLU_2074859_0_0_1"/>
    <dbReference type="InParanoid" id="D5GMH3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ85716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="118" mass="12979" checksum="54DBC80B1E6CA2E2" modified="2010-06-15" version="1">MCTVRVLCTVWHFIQTEYSEFCSHGGASTGVLLVIVFIFPGSHPRGFSEFAGKIAALLCELDHTDHLGGELPTPALPIRHGVKQAFPPLRWDCGRVPDPGIYDTGFLPPNFSVDQKQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMN1</accession>
    <name>D5GMN1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00010822001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ACBP family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430357">
        <property type="protein sequence ID" value="CAZ85774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841583.1">
        <property type="nucleotide sequence ID" value="XM_002841537.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMN1"/>
    <dbReference type="STRING" id="656061.D5GMN1"/>
    <dbReference type="GeneID" id="9185110"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010822001"/>
    <dbReference type="HOGENOM" id="CLU_2074860_0_0_1"/>
    <dbReference type="InParanoid" id="D5GMN1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000062">
        <property type="term" value="F:fatty-acyl-CoA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000582">
        <property type="entry name" value="Acyl-CoA-binding_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035984">
        <property type="entry name" value="Acyl-CoA-binding_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23310:SF62">
        <property type="entry name" value="ACYL-COA BINDING PROTEIN 1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23310">
        <property type="entry name" value="ACYL-COA-BINDING PROTEIN, ACBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00887">
        <property type="entry name" value="ACBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47027">
        <property type="entry name" value="Acyl-CoA binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51228">
        <property type="entry name" value="ACB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACB" evidence="3">
        <location>
            <begin position="7"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ85774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="118" mass="13043" checksum="11211C60ABD8F43E" modified="2010-06-15" version="1">MTSRYNPTSAFYTAAESVGNLAGYDDPGVKLLAYSLYKQTRVGDVTGSRPLLDPESKMKFDAWTLVKGMTHQEADARYLNCSEHMVQGVPLNPENSALLGAIKEKLGVNKPYLSSLWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMP3</accession>
    <name>D5GMP3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00010864001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430358">
        <property type="protein sequence ID" value="CAZ85786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841595.1">
        <property type="nucleotide sequence ID" value="XM_002841549.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMP3"/>
    <dbReference type="STRING" id="656061.D5GMP3"/>
    <dbReference type="GeneID" id="9185122"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010864001"/>
    <dbReference type="eggNOG" id="KOG4234">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_731952_0_0_1"/>
    <dbReference type="InParanoid" id="D5GMP3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052769">
        <property type="entry name" value="TPR_domain_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46014">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46014:SF1">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="378" mass="41307" checksum="4E5C8711B60BB2A4" modified="2010-06-15" version="1">MPPPFLTLLPPSHSHSRRNLASQPIIPNQLIAWHGTLHQNNNISMPPSPPFHHDGCDSDDDEDMFVDSHSHQSASPTTTGMSDTTPPPTPFQSTHEQQQQHPTMERRLGSFSAGSEALGGFVFPSTSDTAERDIREVDGDDDDGHMDTSEDCVATARVAGAREGEEDEHKPMKPPLGERFSKDEENRLITESTSYRISGNRLFNSNFLLPALDAYKLALTSCPIYLVHEVATIYANIAAVELKLQRYPAAVKAATKALEGRPRWGKALYRRARGREGIGGWAELEGALEDYRAVEASAGGAGGAAAGGIGEKELEIVREKIRWLPGRIEEVKDTETAEVMGKLKQMGEGLLKPFGINTSDFGMVKGADGAYSLQFRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMP7</accession>
    <name>D5GMP7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00010871001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430358">
        <property type="protein sequence ID" value="CAZ85790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841599.1">
        <property type="nucleotide sequence ID" value="XM_002841553.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMP7"/>
    <dbReference type="STRING" id="656061.D5GMP7"/>
    <dbReference type="GeneID" id="9185126"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010871001"/>
    <dbReference type="eggNOG" id="KOG1014">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_593379_0_0_1"/>
    <dbReference type="InParanoid" id="D5GMP7"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051019">
        <property type="entry name" value="VLCFA-Steroid_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43899">
        <property type="entry name" value="RH59310P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43899:SF13">
        <property type="entry name" value="RH59310P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ85790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="461" mass="50558" checksum="26A2938D1B734C2F" modified="2010-06-15" version="1">MGYEETNLASAKNHPEFMNRVLGVQNSDAGSARSMTLGRSFLTREWVVVKCRATPLLIFNTGTCTSNRTKRGDKECKGCQPGFRDRITRGLWGAPDNISADSLSTMTPSLTCSYITSHIYALHLLLSTTLHLILQILLSLLSLPTTLHTHLLHHGAKYLEEHYHTPDAFALITGATDGIGRAFALELADRGWNIILHGRTQARINPLLEQLREKYPRQAFLPLPIDAVDARAGGIGQALLMLEPTVRVTLLINNAGAIPPIKAFSELSDEEVIDNVAVNATFGVLVIKALMNGWFARRACVIGMGSVGGVMTLSGTPTYSASKSYTHKFYACLRAQLASPHPASSTTTSSASSAELEILPIIVGGVRTKMTPISDDGNIFLVTPQVFAQHALAKVGLGREVYGYFWHEVQVWCITIWPEEGGMRRWVNSFMMRGVEKLRTEGWRRLNNGGRMVDGRLVGGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMP9</accession>
    <name>D5GMP9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00010874001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430358">
        <property type="protein sequence ID" value="CAZ85792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841601.1">
        <property type="nucleotide sequence ID" value="XM_002841555.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMP9"/>
    <dbReference type="GeneID" id="9185128"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010874001"/>
    <dbReference type="HOGENOM" id="CLU_468667_0_0_1"/>
    <dbReference type="InParanoid" id="D5GMP9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="474"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="49"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="162"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="474"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="531"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="570"/>
            <end position="582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ85792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="582" mass="65246" checksum="9EC0C99C53343B12" modified="2010-06-15" version="1">MQLHRFVMEMAGRFMTGGGHSISGGGSGRTGYEIEDPGDFIRVREAGGKKGYIRELEQLLRESNTANSKLERELQNIERENKDRIRGLEKRLKDCDAANSELERKLQDLKHEKSTLSKEYQNALGQLGTAKDTAKQHRYKCKEISERRDKLHTQCNDLRVKNEGITMANKKQEREIKALRDNNAKITEELETRIGQLNKITGEIARQNQQVNTPAIRDDEYFAREFASLAKHIRNWSFTHLFHHSKTFVPTVVIRDLQGAIGGIASGSNLPEIFKDRAASRRVVSGLLADWLKEHIFDPPLLGLLPGSFLHFEERIKKTGNQKSPWLLQTISLFVQDEEFKRVLNEKVCISSKGLCERLGELLAESDESKVFKRNQKLQAIVERAANLAVEVSQQPYWFCFATIAPGEQFSRYAMEDVGTDMSLEEDDPGTCDDDCRVSLSVFPCVYRYELVEGENRPESIVIHRAWVTVETRDSDKCVEQTDKNITNEEANGGMEPGVECSSGRSGRTEALGGDTGAQGRERGAQEGEESGNSPDPSAPATQNVISTQSTSPESTGGMNHEADLAADVDLGHEKMERESPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMW1</accession>
    <name>D5GMW1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00010963001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430360">
        <property type="protein sequence ID" value="CAZ85854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841663.1">
        <property type="nucleotide sequence ID" value="XM_002841617.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMW1"/>
    <dbReference type="STRING" id="656061.D5GMW1"/>
    <dbReference type="GeneID" id="9183422"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010963001"/>
    <dbReference type="eggNOG" id="ENOG502QUI6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026238_0_0_1"/>
    <dbReference type="InParanoid" id="D5GMW1"/>
    <dbReference type="OMA" id="SVRFMNN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34706:SF2">
        <property type="entry name" value="RFEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34706">
        <property type="entry name" value="SLR1338 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="2">
        <location>
            <begin position="169"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="373"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="26"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="75"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="373"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="389"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="426"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="461"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ85854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="508" mass="54171" checksum="63F01BFFCA3B4D7C" modified="2010-06-15" version="1">MGLASKMAAAQTQGAPPPGGAPPYQQPYGQPAQQPGQYPGYPPAGASPQGPAYAQQQYQPYQGYPPQGQPPHGQPPYGQAPYAGAPPYGQSPAPGYGAPPSGPPPDANILLSILREAVKEKGIENMYPENRLVGIANDMAKKDPVNALCNHWKLPKEIGFDFVKLALFDVVFLLDDSGSMRFGEGLIDELKFILSNVAFATGLFDQDGFSVRFMNSNVQGDNIRTEQQAAALVDQVRFEDVTPLATSLKNKILDQFIYGQEQRNSLKKPVLVIIITDGRPTDNVHGEFQQYIQSVSSHFRGKGVVSFQIAQVGNDKGAQQFLSELDNDKAIGGLIDCTSNFELESMEFRKKGVELTKHLWYTKLLLGSIDRSYDSKDEDKKAANPPGAPGSAGYPAPGGAQPYGQPPGAYGQPPAGKPGQYPPQQPYGAPAAQQYGGGYQHPQGPQPGYGAPHGASPPAPAYGQAPYGQQPGYPPQGQQGYPPQGGYGGPAPPSQYNPNAAYGVPHKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMZ9</accession>
    <name>D5GMZ9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00010992001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430361">
        <property type="protein sequence ID" value="CAZ85874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841683.1">
        <property type="nucleotide sequence ID" value="XM_002841637.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMZ9"/>
    <dbReference type="STRING" id="656061.D5GMZ9"/>
    <dbReference type="GeneID" id="9183442"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010992001"/>
    <dbReference type="eggNOG" id="KOG1351">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097969_0_0_1"/>
    <dbReference type="InParanoid" id="D5GMZ9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046034">
        <property type="term" value="P:ATP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18118">
        <property type="entry name" value="ATP-synt_V_A-type_beta_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022879">
        <property type="entry name" value="V-ATPase_su_B/beta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43389">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43389:SF4">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit N-terminal" evidence="3">
        <location>
            <begin position="39"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ85874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="199" mass="21662" checksum="515C18F0D05EB807" modified="2010-06-15" version="1">MAPGVDDPRISAQEAFAINAAAVTRNYGVNPRINYNTVAGVNGPLVILDNVKFPRYNEIVNLTLPDGTKRAGQVLEVRGSRAIVQVFEGTPGIDVRKTTVEFTGQNLKIPVSEDMLGRIFDGSGRAIDKGPKVFAEDYLDINGSPINPYSRVYPEEMISTGISAIDTMNSPVVKKSPSSQPLAFPTTKLLRRFAVKPVW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-12-14" modified="2025-06-18" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5GN29</accession>
    <name>TRM5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA (guanine(37)-N(1))-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.228</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">M1G-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA [GM37] methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA methyltransferase 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">TRM5</name>
        <name type="ORF">GSTUM_00011062001</name>
    </gene>
    <organism>
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>guanosine(37) in tRNA + S-adenosyl-L-methionine = N(1)-methylguanosine(37) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36899"/>
            <dbReference type="Rhea" id="RHEA-COMP:10145"/>
            <dbReference type="Rhea" id="RHEA-COMP:10147"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="EC" id="2.1.1.228"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="1">Predominantly in the mitochondria and in the nucleus.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.228" evidence="1"/>
    <dbReference type="EMBL" id="FN430362">
        <property type="protein sequence ID" value="CAZ85922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841731.1">
        <property type="nucleotide sequence ID" value="XM_002841685.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GN29"/>
    <dbReference type="FunCoup" id="D5GN29">
        <property type="interactions" value="1076"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GN29"/>
    <dbReference type="EnsemblFungi" id="CAZ85922">
        <property type="protein sequence ID" value="CAZ85922"/>
        <property type="gene ID" value="GSTUM_00011062001"/>
    </dbReference>
    <dbReference type="GeneID" id="9185848"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011062001"/>
    <dbReference type="eggNOG" id="KOG2078">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022610_2_2_1"/>
    <dbReference type="InParanoid" id="D5GN29"/>
    <dbReference type="OMA" id="VGSHSQF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052906">
        <property type="term" value="F:tRNA (guanine(37)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070901">
        <property type="term" value="P:mitochondrial tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002939">
        <property type="term" value="P:tRNA N1-guanine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.110:FF:000001">
        <property type="entry name" value="tRNA (guanine(37)-N1)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.110">
        <property type="entry name" value="Met-10+ protein-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03152">
        <property type="entry name" value="TRM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030382">
        <property type="entry name" value="MeTrfase_TRM5/TYW2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056743">
        <property type="entry name" value="TRM5-TYW2-like_MTfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056744">
        <property type="entry name" value="TRM5/TYW2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025792">
        <property type="entry name" value="tRNA_Gua_MeTrfase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245:SF36">
        <property type="entry name" value="TRNA (GUANINE(37)-N1)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245">
        <property type="entry name" value="TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02475">
        <property type="entry name" value="TRM5-TYW2_MTfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25133">
        <property type="entry name" value="TYW2_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51684">
        <property type="entry name" value="SAM_MT_TRM5_TYW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-0496">Mitochondrion</keyword>
    <keyword id="KW-0539">Nucleus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000414171" description="tRNA (guanine(37)-N(1))-methyltransferase">
        <location>
            <begin position="1"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="287"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="287"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="244"/>
            <end position="245"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="272"/>
            <end position="273"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="428" mass="47946" checksum="1B31F686A1A98F1F" modified="2010-06-15" version="1">MSRLFLPPINRAMRTLDPSFFQKTIPLTAAHVLDGRNIPRVTKECARDLLRVPRLRSVITQKDDAGMERKLLLLRPEVKHDDLSTAQPKTREFVEERIIKLEPYTLHMKYESWNYEEIMNAILPEDLEEIPCAFTQAGHLAHLNLREQYLPYKHLIATVLLDKNPNVSTVVNKIEDVGTGSVYRTFPMELLAGQDNTNVEVRESGCVFRFDFAKPGEAVADVMAGVGPFAIPAAKQRVFVYANDLNPESYKSLVGNIHVNKVSQFLTPHNLDGANFIRESIRTLLSRSRNPSTSKVSIPTPGKRGQKRPPPPTVIPVPPTFSHFVMNLPASATTFLGAFRGAYRGLEDVVTGQEMLPIIHVYTFHKSSVTENAAGDICADISRHLGREMGEGDLENLENVRLVSPNKTYYCVSFRLPANVAFEDAQVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNB8</accession>
    <name>D5GNB8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00011212001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the WD repeat WDR3/UTP12 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430363">
        <property type="protein sequence ID" value="CAZ86011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841820.1">
        <property type="nucleotide sequence ID" value="XM_002841774.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNB8"/>
    <dbReference type="FunCoup" id="D5GNB8">
        <property type="interactions" value="1129"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GNB8"/>
    <dbReference type="GeneID" id="9182211"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011212001"/>
    <dbReference type="eggNOG" id="KOG0306">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005318_0_1_1"/>
    <dbReference type="InParanoid" id="D5GNB8"/>
    <dbReference type="OMA" id="MNIPLTC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034388">
        <property type="term" value="C:Pwp2p-containing subcomplex of 90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000157">
        <property type="entry name" value="WD repeat domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000178">
        <property type="entry name" value="WD repeat domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007148">
        <property type="entry name" value="SSU_processome_Utp12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051570">
        <property type="entry name" value="TBC1_cilium_biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19853">
        <property type="entry name" value="WD REPEAT CONTAINING PROTEIN 3 WDR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19853:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25173">
        <property type="entry name" value="Beta-prop_WDR3_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25172">
        <property type="entry name" value="Beta-prop_WDR3_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04003">
        <property type="entry name" value="Utp12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117289">
        <property type="entry name" value="Nucleoporin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 6" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="84"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="111"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="181"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="260"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="406"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="487"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="586"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="628"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="670"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="domain" description="Small-subunit processome Utp12" evidence="8">
        <location>
            <begin position="805"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="304"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="721"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="312"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ86011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="940" mass="104353" checksum="91B732B421A840BE" modified="2010-06-15" version="1">MVKSYLRFEHSSTFGVIASGTANTVWTAGATTPAQTKTTSAGRAVAPANEEVLTWDIKKGELLSRWRDGKCKAEVSAIAQSQADKDVFAVGYADGSIRLWDSKTSAVVISFNGHRSAVSTLAFDHSGTRLASGSKDAHVIVWDLISEVGLYRLKGHKDQVTKVGFLKGPEVVEGGSAEGWLVSTGKDTLIKLWDLTTQHCVETHVAHHGECWAMALSPDERGCITAGNGGEMKVWAVDTAGLTSRVKGKSDCLIDRGTIYRQNKDKAISITFHPNSNFVASHGADKSVEIWRIRTEDEVKKALKRKRKRKEGKANKRDKAEKTEEECDNVPYAEVADIFVSYVTVRTGGKVRSVDWTTRGGKDTVQILVACTNNTLEYYDIRKPARKEGRKEQEVPEYNKLYAVELSGHRTDIRALSLSSDDRMLASASSGSLKIWNVRTSTCIRTFECGYALSCSFLPGDKIVVVGTKTGEIELFDVASSAMIESIKAHEASIWSLQVHPDGKSLVTGSADKNAKFWSFQIAQESIPGTKRTTPRLKLVQSRALKLSDDILSVRYSPNGKLLAVALLDNTVRVFYVDTLKRLLNLYGHKLPVLSMDISSDSKLIATCSADKNVKIWGLDFGDCHKSFFAHQDSVMQVAFERNSHNFLSAGKDRLIKYWDGDKFENIIKMDSHFGEIWALAVGKVSEIVVSASHDKSIRVWEQTDDEIFLEEEREKELEELYESTLTTSLNQDETQDENGPEATSAGKQTIETLMAGEKIIEALDLGMADMTMMKEYAAAKIEKPNLAPPPRNPLYIALGGISAEMHLLNTIQKIKASHLQDALLVLPFDRVALFLIFLDIFASKQWNIPLTCRILFFLLKTHHKQIVATKTMRVLLSSVRKNLRTALKSQKDQMGFNLAAVRFVKNGVEAAGKSDFVDEEAFREMEERGKRKRAFATLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNL2</accession>
    <name>D5GNL2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein uL11m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00011350001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the universal ribosomal protein uL11 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430366">
        <property type="protein sequence ID" value="CAZ86105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841914.1">
        <property type="nucleotide sequence ID" value="XM_002841868.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNL2"/>
    <dbReference type="FunCoup" id="D5GNL2">
        <property type="interactions" value="611"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GNL2"/>
    <dbReference type="GeneID" id="9186181"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011350001"/>
    <dbReference type="eggNOG" id="KOG3257">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074237_1_0_1"/>
    <dbReference type="InParanoid" id="D5GNL2"/>
    <dbReference type="OMA" id="CKQFNAK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00349">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.250:FF:000005">
        <property type="entry name" value="Mitochondrial 54S ribosomal protein YmL19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1550.10:FF:000004">
        <property type="entry name" value="Mitochondrial 54S ribosomal protein YmL19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.250">
        <property type="entry name" value="Ribosomal protein L11, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1550.10">
        <property type="entry name" value="Ribosomal protein L11/L12, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00736">
        <property type="entry name" value="Ribosomal_uL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000911">
        <property type="entry name" value="Ribosomal_uL11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006519">
        <property type="entry name" value="Ribosomal_uL11_bac-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020783">
        <property type="entry name" value="Ribosomal_uL11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036769">
        <property type="entry name" value="Ribosomal_uL11_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020784">
        <property type="entry name" value="Ribosomal_uL11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036796">
        <property type="entry name" value="Ribosomal_uL11_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01632">
        <property type="entry name" value="L11_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL11M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00298">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03946">
        <property type="entry name" value="Ribosomal_L11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00649">
        <property type="entry name" value="RL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54747">
        <property type="entry name" value="Ribosomal L11/L12e N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46906">
        <property type="entry name" value="Ribosomal protein L11, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 8" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL11 N-terminal" evidence="10">
        <location>
            <begin position="12"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL11 C-terminal" evidence="9">
        <location>
            <begin position="75"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ86105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="154" mass="16549" checksum="8BBF73DFD2837BF0" modified="2010-06-15" version="1">MSRAVARKNQIVKLIVGAGQASPSPPVGPALGSKGVKSMDFCKEFNARTAHITPGTPTPVRITVRPDRSFTFDVRTPPTSWLLKKAAALPLDKKGRMKGASNPGKEWVGEIGLKHVYEIAKIKQTEVRLSGLSLRAIAGCVIHQARTIGIKIAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNN1</accession>
    <name>D5GNN1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00011383001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430366">
        <property type="protein sequence ID" value="CAZ86124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841933.1">
        <property type="nucleotide sequence ID" value="XM_002841887.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNN1"/>
    <dbReference type="GeneID" id="9186200"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011383001"/>
    <dbReference type="eggNOG" id="ENOG502S41P">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_553421_0_0_1"/>
    <dbReference type="InParanoid" id="D5GNN1"/>
    <dbReference type="OMA" id="IMADAYK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43102:SF2">
        <property type="entry name" value="GAF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43102">
        <property type="entry name" value="SLR1143 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55781">
        <property type="entry name" value="GAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="170"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ86124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="493" mass="54592" checksum="1B31A840D5710A42" modified="2010-06-15" version="1">MKPLRSSKPSMRSIGNMLTEMRALGLTCCCQMSRWLYWMLQRCVTIKVMCRPTLIIPQDWRLKGNPLVKSAPYIRFYAGAPIITFDGRTIGAFSVFDDTPRDSFGVGERRKLMDFARLAMTEIENVMEERDMLSRRTPTPIIDLRVLHEQDKQSLVGDWPKTSRDGTPVSPKSSSPKLAQNPASSRTARKRDAPTPVNRPAAIRPGGRLPTINIPVEPEDARNSRTSAKISDMPTPPHTPSRPFSFTTNNSRPLSLTAPPGSVDVASSPDSPEGWSADRRAHYKQRKPPPIQTPGPREVPLHIPFSSGPITSLAESSFATSIIARSLNYDFVYLLRVSPVTSQEPRILPFEQEYAGDNFHTKVLVAHGLPNPLPVFDANLHLRALRSVGGLVYQNPAKSQDEDDVGFSSGILLPLLRDGIDNSPDEGYSSATTTIGGGFQQSETTCTGGIVLAAFTRKDEGDHRPPFTADEVRCLREFGEAMKDILLRVDRKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPD5</accession>
    <name>D5GPD5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="11">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00011683001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Vacuole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the laat-1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430373">
        <property type="protein sequence ID" value="CAZ86297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842106.1">
        <property type="nucleotide sequence ID" value="XM_002842060.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPD5"/>
    <dbReference type="FunCoup" id="D5GPD5">
        <property type="interactions" value="294"/>
    </dbReference>
    <dbReference type="GeneID" id="9186537"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011683001"/>
    <dbReference type="eggNOG" id="KOG2913">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019699_1_0_1"/>
    <dbReference type="InParanoid" id="D5GPD5"/>
    <dbReference type="OMA" id="DMCIFIQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098588">
        <property type="term" value="C:bounding membrane of organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015174">
        <property type="term" value="F:basic amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015101">
        <property type="term" value="F:organic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034488">
        <property type="term" value="P:basic amino acid transmembrane export from vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034490">
        <property type="term" value="P:basic amino acid transmembrane import into vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000011">
        <property type="entry name" value="PQ loop repeat protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000012">
        <property type="entry name" value="Vacuolar membrane PQ loop repeat protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051415">
        <property type="entry name" value="LAAT-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201">
        <property type="entry name" value="SEVEN TRANSMEMBRANE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201:SF35">
        <property type="entry name" value="VACUOLAR AMINO ACID TRANSPORTER YPQ1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00679">
        <property type="entry name" value="CTNS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="4" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="49"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="192"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="229"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="270"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ86297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="307" mass="33715" checksum="8A5C1F00E77657EA" modified="2010-06-15" version="1">MFPPAQQLDIDARAISGIAGSISIACWIVVFSPQIIANFQRGSANGLSLAFLIVWLAGDVFNVLGAVLQGVLPTMVMLAVYYTLADVVLLFQCLYYSCINSRNAAPEAESLLPVSNESAATVDGSHLSPATPLLDPAKEPIHTEPQRLMKSVYFNALSILIVCLAGVFGWWASNRYSDCDPPPNGHGDELSFSALGQTFGYLCAVLYLASRVPQILLNYRRQSCEGISLLFFLFACIGNLTYVVSILAYIPHSRDEPGQEYERYIAVNASWLLGSVGTLFLDLIIFAQFFWYRSTEEEYESSSEDDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPD8</accession>
    <name>D5GPD8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00011686001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430373">
        <property type="protein sequence ID" value="CAZ86300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842109.1">
        <property type="nucleotide sequence ID" value="XM_002842063.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPD8"/>
    <dbReference type="FunCoup" id="D5GPD8">
        <property type="interactions" value="60"/>
    </dbReference>
    <dbReference type="GeneID" id="9186540"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011686001"/>
    <dbReference type="eggNOG" id="KOG2615">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_54_5_1"/>
    <dbReference type="InParanoid" id="D5GPD8"/>
    <dbReference type="OMA" id="AWWACGI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17330">
        <property type="entry name" value="MFS_SLC46_TetA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504:SF15">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="52"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="86"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="144"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="186"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="296"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="350"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="382"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="406"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="441"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="481"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="14"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ86300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="574" mass="62047" checksum="1B8048FE989125BC" modified="2010-06-15" version="1">MAATLPRKEFPTRQLFVLSLCRICEPISFCSILPYIYYMIESFGCAKNPNEVAVYAGMVTSSFAFAEFTTGMMWGRVSDKFGRKPVLIMGLVGTMLSMLIFGFAKSFPVALIARAVGGALNGNVGVLQTTVAELATEREHQARAFAVMPFVWCLGSIVGPALGGLLADPVKQYPAMFHPGGVLEEYPYLLPNLVSANILAVGIIIGTLFLEETHEILREKPDIGVRTGRKIVKFFKRRSSGGKFEGPEVCGSPPKDGEERLLASGSSEYSTFPNALATNITEEEQVRKGPPIIKTFTAPVVLLIVSYGILAYHTMGFEQLFPVFLSTPRSAEPPHHLLKFTGGFGLDTQTIGFILSVQGAVSMAIQFLFFPPIVEHFGTLSVYRFCMFLYPISYLIVPYIDFLPPSLSMVGVCGVLLVRITFGVLAYPCNAILLTNAAPSLLVLGAINGVAASAASISRALGPTITGMIYTQGLDWGMVGLAWWVNAGVCVLGGIQCLWMKEEDFDGNNKTGEIVIDEEVCWRVAAETQAAHAHNTGLVSGEEFISECTIARKTAGEEVANSFGEEGDMGGRSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPG2</accession>
    <name>D5GPG2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00011832001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430375">
        <property type="protein sequence ID" value="CAZ86405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842214.1">
        <property type="nucleotide sequence ID" value="XM_002842168.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPG2"/>
    <dbReference type="FunCoup" id="D5GPG2">
        <property type="interactions" value="317"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GPG2"/>
    <dbReference type="GeneID" id="9186645"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011832001"/>
    <dbReference type="eggNOG" id="KOG2402">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019525_0_0_1"/>
    <dbReference type="InParanoid" id="D5GPG2"/>
    <dbReference type="OMA" id="ISGCYAR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016593">
        <property type="term" value="C:Cdc73/Paf1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990269">
        <property type="term" value="F:RNA polymerase II C-terminal domain phosphoserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.200">
        <property type="entry name" value="Plus-3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004343">
        <property type="entry name" value="Plus-3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036128">
        <property type="entry name" value="Plus3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13115">
        <property type="entry name" value="RNA POLYMERASE-ASSOCIATED PROTEIN RTF1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13115:SF8">
        <property type="entry name" value="RNA POLYMERASE-ASSOCIATED PROTEIN RTF1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03126">
        <property type="entry name" value="Plus-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00719">
        <property type="entry name" value="Plus3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159042">
        <property type="entry name" value="Plus3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51360">
        <property type="entry name" value="PLUS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Plus3" evidence="7">
        <location>
            <begin position="248"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="149"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="499"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="422"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="39"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="67"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="86"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="153"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="181"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="503"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="514"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ86405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="567" mass="63611" checksum="7DBD09D4B9C2C0CC" modified="2010-06-15" version="1">MSLDNELLMLAGEEDEDDFKPSSPPRRKRTSSPNSVSPAKKRAKPTVRKTNKKKTRRDSDDKGNEDSSGLSDAPSSSEEGFRSEAEESMDLESSSDEEDSVQDLYPLEGKYKDQADMRALLAMNEIDREALLAERAGLIERAQQDRHLRNLLKSRNTADTANGTSVSGANRRSTRTKSMPKKTEETTKRGKLDELKKIREERITRAAGSGKQTGDEDDARARRRSINEDDEDKAGYNEEIYVKEDDRDIVLADVNKARIGRTGLAKLCDYPGFEEVVTDCFVRVSLFDRETGTTSYRLGWVKGFVSGKYYNMLDGKRRTDQHLRVSQGLAERSFGMEFMSDSPITEAELNRFKKQMEFDHCAMPPLSLLEKKSRELRELDNRVLTQDEVNEMIRKRNSDKVDTVNIVLKRGRLRDARESAQLKGDEAEVARIDKELQDLGDQHGKSLVVPKSQMERLAKLNAVNRKKNVAEIRKAEIEEKRAARTAAVHNPFMRVKTVAKTRHNLDGGKKEGGDSQNASGDSQTDTESKSSPAGVGFLSSSTKGRGRLGASVDDVIANYDFGIEIDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPG7</accession>
    <name>D5GPG7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00011839001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430375">
        <property type="protein sequence ID" value="CAZ86410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842219.1">
        <property type="nucleotide sequence ID" value="XM_002842173.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPG7"/>
    <dbReference type="STRING" id="656061.D5GPG7"/>
    <dbReference type="GeneID" id="9186650"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011839001"/>
    <dbReference type="eggNOG" id="KOG4582">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007590_0_0_1"/>
    <dbReference type="InParanoid" id="D5GPG7"/>
    <dbReference type="OMA" id="NKHGHHP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14947">
        <property type="entry name" value="NBR1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02249">
        <property type="entry name" value="ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02340">
        <property type="entry name" value="ZZ_NBR1_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.90">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032350">
        <property type="entry name" value="Nbr1_FW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043145">
        <property type="entry name" value="Znf_ZZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20930">
        <property type="entry name" value="OVARIAN CARCINOMA ANTIGEN CA125-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20930:SF0">
        <property type="entry name" value="PROTEIN ILRUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16158">
        <property type="entry name" value="N_BRCA1_IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00569">
        <property type="entry name" value="ZZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00291">
        <property type="entry name" value="ZnF_ZZ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZZ-type" evidence="5">
        <location>
            <begin position="249"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="ZZ-type" evidence="5">
        <location>
            <begin position="326"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="ZZ-type" evidence="5">
        <location>
            <begin position="396"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="ZZ-type" evidence="5">
        <location>
            <begin position="459"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="101"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="729"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="790"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="107"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ86410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="845" mass="92042" checksum="10FE3A7C36F15EB7" modified="2010-06-15" version="1">MASHGTVHPDTAIKTKVTFNGNTRCFKLVLRDLGANVLLPKLRTLLAIPEDKQVVFERYSDSAGGYVILDTAEPTAFKQLYRAARAKLKLRIQATIVPAEVPAPVDPSSSTDTLTGQRNVPSTEAGPSTQPSPSNVSHFSLPPAYASKVSLVPVETPFEELATVDTTTKFAPPADLGQAVADAVSTYLSGDEYLQKLRETVREEVEKLEKSTEKLEQAPLEAPVPAPFTAPPTDISEFENFETKVAPSPFTYAIYCNSCETSIKDVHYHCGCCDGGDYDLCKGCVARGIHCKDSSHWLIKRVLSPTGVIVSSTTESVSQKDGSVQTSSRRTCNCCVVDLPSKEFVTCKVCEDYDVCISCHTSLKHGHNPSHEFVPAAPDTMLQPYAISLLAPGRAHRHYAVCDGCDKQIYGVRNKCLSCPDWDYCANCISAAPENHPGHRFVPIYEPLPVGFINTDFVRHFGIYCDGPMCADKTNRSWIVGDRYKCAVCPDTDFCASCEASPTNPHNATHPLIKLKTPVRNVHVTTMENQDSENILGDVPIPVPTTNAATQVQSVADAKPVEEPEPPFENVEEYLEKTVLAAEEGKFEPEAEPKIRAGPEPEPVVPEPEPLQATFVRDTIPDGCEVIAGDEFTQTWILNNSGSSTWPAGVSIQFVCGDEMFSNNEGSSIGATVSSTETAPGNNAAFSVNLKAPLVTNRRLITYWRLVGPDGSRFGDKLWCEIQSIERPKEDKMEQSVLESKDEFEDVSEHANDGSQASSQMIFPKLPVESPIASTENLVAGVSLKGENMPVSVPMSKTSTRMGDDESEVDVGSLGDEVESFLTDDEYDVLDASDEEAFEECEKAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPR9</accession>
    <name>D5GPR9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00011998001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL38 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430377">
        <property type="protein sequence ID" value="CAZ86512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842321.1">
        <property type="nucleotide sequence ID" value="XM_002842275.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPR9"/>
    <dbReference type="FunCoup" id="D5GPR9">
        <property type="interactions" value="802"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GPR9"/>
    <dbReference type="GeneID" id="9183575"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011998001"/>
    <dbReference type="eggNOG" id="KOG3499">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_152057_1_0_1"/>
    <dbReference type="InParanoid" id="D5GPR9"/>
    <dbReference type="OMA" id="RCHRFIY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022618">
        <property type="term" value="P:protein-RNA complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.720.90:FF:000001">
        <property type="entry name" value="60S ribosomal protein L38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002675">
        <property type="entry name" value="Ribosomal_eL38"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038464">
        <property type="entry name" value="Ribosomal_eL38_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10965">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10965:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01781">
        <property type="entry name" value="Ribosomal_L38e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ86512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="78" mass="8891" checksum="775CB6F666CBA783" modified="2010-06-15" version="1">MPKQVHDIKQFLELCKRDDAKAARIKKPTKGSNTIKFKVRCHRHLYTLVLHDAEKAEKLKQSLPPALVTTDVPATSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5G434</accession>
    <name>D5G434_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Dihydrofolate synthetase</fullName>
            <ecNumber evidence="7">6.3.2.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00003931001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>7,8-dihydropteroate + L-glutamate + ATP = 7,8-dihydrofolate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23584"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17839"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57451"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Cofactor biosynthesis; tetrahydrofolylpolyglutamate biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the folylpolyglutamate synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.12" evidence="7"/>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835156.1">
        <property type="nucleotide sequence ID" value="XM_002835110.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G434"/>
    <dbReference type="FunCoup" id="D5G434">
        <property type="interactions" value="178"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G434"/>
    <dbReference type="GeneID" id="9182404"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003931001"/>
    <dbReference type="eggNOG" id="KOG2525">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015869_1_1_1"/>
    <dbReference type="InParanoid" id="D5G434"/>
    <dbReference type="OMA" id="SIHDRIC"/>
    <dbReference type="UniPathway" id="UPA00850"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008841">
        <property type="term" value="F:dihydrofolate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004326">
        <property type="term" value="F:tetrahydrofolylpolyglutamate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="entry name" value="Mur ligase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="entry name" value="Mur-like, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001645">
        <property type="entry name" value="Folylpolyglutamate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018109">
        <property type="entry name" value="Folylpolyglutamate_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01499">
        <property type="entry name" value="folC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136:SF0">
        <property type="entry name" value="DIHYDROFOLATE SYNTHETASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136">
        <property type="entry name" value="FOLYLPOLYGLUTAMATE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001563">
        <property type="entry name" value="Folylpolyglu_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="MurD-like peptide ligases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="MurD-like peptide ligases, peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01012">
        <property type="entry name" value="FOLYLPOLYGLU_SYNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mur ligase central" evidence="8">
        <location>
            <begin position="26"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR001563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ79277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="415" mass="44088" checksum="11B9C5248CAEA8E9" modified="2010-06-15" version="1">MIDLGLKRIARLVRDHGKFSWRAIHVAGTNGKGSVCAYISSCLESSGIPSARFTSPHLVDRRDCITVNGALVNEELFLAAEAAVKQKNAQEKIGASEFEILTATAFEIFSRERVDLAVIEVGIGGRLDATNVIKSPIAAVITKIGLDHQAFLGSSLEEIAGEKGGVIKAGSRCIVDGTNEERVLSVLGKMAEEAGVESFHAVRPEMVGEKGGVDGRICEITTEAFGRQRFEGFLKGNYQPANLSCAVHVLSYLSMEYPSITASTVLAGVRNTSWPGRMDTIDLSCLFPGQERRVLLDGAHNIQAALALSDYVTKELRTGGRFVSWNIAASKGKDVKEMLGILLRPGDKVVAVEFGPVDGMPWVTATSTGDIIGAVREAFGEEIEAVDGGKDVLGGVKAALELAGDEGRTVVAGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>D5G457</accession>
    <name>D5G457_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Mediator of RNA polymerase II transcription subunit 18</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7 8">Mediator complex subunit 18</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">MED18</name>
        <name evidence="9" type="ORF">GSTUM_00003959001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Mediator complex subunit 18 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835179.1">
        <property type="nucleotide sequence ID" value="XM_002835133.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G457"/>
    <dbReference type="STRING" id="656061.D5G457"/>
    <dbReference type="GeneID" id="9182427"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003959001"/>
    <dbReference type="eggNOG" id="ENOG502S7EN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084516_0_0_1"/>
    <dbReference type="InParanoid" id="D5G457"/>
    <dbReference type="OMA" id="PVHQHHE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.320.10">
        <property type="entry name" value="Hypothetical Protein Pfu-838710-001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019095">
        <property type="entry name" value="Mediator_Med18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13321:SF2">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13321">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION, SUBUNIT 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09637">
        <property type="entry name" value="Med18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ79300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="248" mass="27636" checksum="34894DD6B9C4692C" modified="2010-06-15" version="1">MPPPSHPPYAQSTYHELSLYSHIPAARQPQILRVISGITGMLPETTLEHHLVYKPKRPRQPGQNAELYYLQLISIVPGEQKQEEEEEGKNGYDVKSQKWRMRLEDIPEVTRRPVTSRNVLGAGFGEGDALGLVDSLGFMLQTTYFMTVSTLIHNNIIITLSQVLLPPPPPPLPAAPNVQYGNPYPTSGLQPLDPAGSWVLKAAVRVQSHQDVESINTGTGELKVFKELMRGMCDLEVAERLALDTRIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>D5G484</accession>
    <name>D5G484_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00003997001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835206.1">
        <property type="nucleotide sequence ID" value="XM_002835160.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G484"/>
    <dbReference type="STRING" id="656061.D5G484"/>
    <dbReference type="GeneID" id="9184952"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003997001"/>
    <dbReference type="HOGENOM" id="CLU_1435413_0_0_1"/>
    <dbReference type="InParanoid" id="D5G484"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012942">
        <property type="entry name" value="SRR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42080">
        <property type="entry name" value="SRR1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42080:SF1">
        <property type="entry name" value="SRR1-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07985">
        <property type="entry name" value="SRR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="SRR1-like" evidence="2">
        <location>
            <begin position="6"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ79327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="189" mass="20703" checksum="B3E0186638C0CFFE" modified="2010-06-15" version="1">MLFPRARELNRLVVFGLGSFSGLTASSTYYQFALVREVAELLKIRKGEVYFQDPLFTEWDRAFLEGVGSVVEVGSGEEVVQEGTVVVAVHLEYGVLEGVLEGRPGVAVCNDLGHFLDMRIGGEVEWVRAFLEGVEGEVLKWDIGSGGAFNHSAVYWRKGAEDCREVESDKVDGENAVEAVIENIGSVNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5G486</accession>
    <name>D5G486_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Cysteine proteinase 1, mitochondrial</fullName>
            <ecNumber evidence="2 9">3.4.22.40</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00003999001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Has aminopeptidase activity, shortening substrate peptides sequentially by 1 amino acid. Has bleomycin hydrolase activity, which can protect the cell from the toxic effects of bleomycin. Has homocysteine-thiolactonase activity, protecting the cell against homocysteine toxicity.</text>
    </comment>
    <comment type="function">
        <text evidence="7">The normal physiological role of the enzyme is unknown, but it is not essential for the viability of yeast cells. Has aminopeptidase activity, shortening substrate peptides sequentially by 1 amino acid. Has bleomycin hydrolase activity, which can protect the cell from the toxic effects of bleomycin. Has homocysteine-thiolactonase activity, protecting the cell against homocysteine toxicity. Acts as a repressor in the GAL4 regulatory system, but this does not require either the peptidase or nucleic acid-binding activities.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>Inactivates bleomycin B2 (a cytotoxic glycometallopeptide) by hydrolysis of a carboxyamide bond of beta-aminoalanine, but also shows general aminopeptidase activity. The specificity varies somewhat with source, but amino acid arylamides of Met, Leu and Ala are preferred.</text>
            <dbReference type="EC" id="3.4.22.40"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homohexamer. Binds to nucleic acids. Binds single-stranded DNA and RNA with higher affinity than double-stranded DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peptidase C1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.40" evidence="2 9"/>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835208.1">
        <property type="nucleotide sequence ID" value="XM_002835162.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G486"/>
    <dbReference type="FunCoup" id="D5G486">
        <property type="interactions" value="677"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G486"/>
    <dbReference type="MEROPS" id="C01.085"/>
    <dbReference type="GeneID" id="9184954"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003999001"/>
    <dbReference type="eggNOG" id="KOG4128">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038600_0_1_1"/>
    <dbReference type="InParanoid" id="D5G486"/>
    <dbReference type="OMA" id="QSYTFFW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070005">
        <property type="term" value="F:cysteine-type aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043418">
        <property type="term" value="P:homocysteine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009636">
        <property type="term" value="P:response to toxic substance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00585">
        <property type="entry name" value="Peptidase_C1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004134">
        <property type="entry name" value="Peptidase_C1B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10363">
        <property type="entry name" value="BLEOMYCIN HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10363:SF2">
        <property type="entry name" value="BLEOMYCIN HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03051">
        <property type="entry name" value="Peptidase_C1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005700">
        <property type="entry name" value="PepC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 9" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0788">Thiol protease</keyword>
    <feature type="active site" evidence="10">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="399"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00026080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR005700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR005700-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ79329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="478" mass="53108" checksum="04A698254DD08AA7" modified="2010-06-15" version="1">MSDLTLCDTDSSEEPEPSLSLRLEAAIKWEAKLLSEPKNRLALAALSTHASTAILQKPSALLHDTHVFSDQIELEGTPVTNQRSSGRCWLFASTNVFRVPIIKKYKLTGFELSQNYLFFWDKLEKANFFLEQIINTQEADLDGRLIQYLLQSPVGDGGQWDMAVNLVEKYGLIPQAFYPDSFNSKSSGRINWLITAKLREAALTLRSLGASCKSGELEIYKAKVLQEIYGVLVLSLGVPPKPDDTFTWNFHDKDGKFRSITTTPLEFYKENTGTISYGAGVIDGVLGGGDRGGVAERFSLVNDPRNEYNRLLTVERLGNVVGGRGVKYVNVSTDVMKSAAIAMIKAQRPVFFGCDVGKFAEGGRGIMDTGLFDYELGYNITLYMNKAERLRTGESSMTHAMVLSGVNIVDGKPTKWRVENSWGAESGEKGYWLMSDAWFDEYCYQVVTDPAFVSNEVLNVLKQDPISLPIWDPMGSLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4N9</accession>
    <name>D5G4N9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00000030001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429989">
        <property type="protein sequence ID" value="CAZ79482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835325.1">
        <property type="nucleotide sequence ID" value="XM_002835279.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4N9"/>
    <dbReference type="STRING" id="656061.D5G4N9"/>
    <dbReference type="MEROPS" id="M80.A03"/>
    <dbReference type="GeneID" id="9188418"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000030001"/>
    <dbReference type="eggNOG" id="KOG4842">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025898_1_0_1"/>
    <dbReference type="InParanoid" id="D5G4N9"/>
    <dbReference type="OMA" id="VLPEHLC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07344">
        <property type="entry name" value="M48_yhfN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2010.10">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013536">
        <property type="entry name" value="WLM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053000">
        <property type="entry name" value="WSS1-like_metalloprotease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46622">
        <property type="entry name" value="DNA-DEPENDENT METALLOPROTEASE WSS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46622:SF1">
        <property type="entry name" value="DNA-DEPENDENT METALLOPROTEASE WSS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08325">
        <property type="entry name" value="WLM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51397">
        <property type="entry name" value="WLM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WLM" evidence="2">
        <location>
            <begin position="7"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="170"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="263"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="307"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="196"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="272"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ79482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="448" mass="49209" checksum="F479EA2C09DB6CFA" modified="2010-06-15" version="1">MTLGINPRKRPPNANITFITALPGEDYDVALDYLERIAAIVFPVMRDNGLAVMSLDEFPATMEFWGRNFNAGEVIQLVLKNPNTGAWLPFSFVQRVMIHELAHIKQMNHSRSFWSVNSKFSTQLQVLRAKGYTGEGFWSAGRTLLSERHTHDQPLAEDDMPKSLCGGTYRSYEKRKSTQDANYRAKKPKLTYAERQQYRKERKFGPSEGEKVGADDNTRSVLEHGKKSTAAPRIAQSARGRELRAAAALKRFEVLKVERVEGGVGDESDSNTESKGDWDEDEKKVDVGGGKYLDACGHGTAWECFDEESTGMSGKGKGREQPRGSTSNNSAKKDAVDLTNDDSQAPPPNCKPQTLNPPLALPNPQNPILSLSSRPKAKAPNEASKTTCTACSFANDPTSTLCCVCANVLNPGRMKNAWKCSCYGDLRYMNPGDAGICGVCGGRGGRGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4R7</accession>
    <name>D5G4R7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00000067001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429990">
        <property type="protein sequence ID" value="CAZ79503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835346.1">
        <property type="nucleotide sequence ID" value="XM_002835300.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4R7"/>
    <dbReference type="STRING" id="656061.D5G4R7"/>
    <dbReference type="GeneID" id="9188439"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000067001"/>
    <dbReference type="eggNOG" id="KOG1843">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3771">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025518_1_0_1"/>
    <dbReference type="InParanoid" id="D5G4R7"/>
    <dbReference type="OMA" id="MLAHYYT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031097">
        <property type="term" value="C:medial cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990528">
        <property type="term" value="C:Rvs161p-Rvs167p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051666">
        <property type="term" value="P:actin cortical patch localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097320">
        <property type="term" value="P:plasma membrane tubulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000100">
        <property type="entry name" value="SH3 domain-containing YSC84-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046982">
        <property type="entry name" value="BIN3/RVS161-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47174">
        <property type="entry name" value="BRIDGING INTEGRATOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47174:SF2">
        <property type="entry name" value="SH3 DOMAIN SIGNALLING PROTEIN (AFU_ORTHOLOGUE AFUA_5G07670)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03114">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51021">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="BAR" evidence="5">
        <location>
            <begin position="7"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="554"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="289"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="328"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="335"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="407"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="450"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="498"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="515"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="537"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ79503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="615" mass="67698" checksum="E39BA312571E8FA6" modified="2010-06-15" version="1">MTTTIHRQIGKLGKRSADEATVAMLIKEVNDAEVLLGKLIEHTKAWRDSWTEILVDQTILTDSFHDIYKTIPLPQDYNIQVAETPVPVLRKVAKLHAAQNDLKTDMTEEIERVDKMLVERLSEAKAALKPIKKAIDKRENKKLDFERFTKTVENQRGKKNKSDRDYSVLSKAELDLERAQNAYENADNHLKDWVPQMMTKITEFLPCVVESIVQTQYTLLSNTYSAIYEYANEHNFTDPDGEVVIAEWEDLFIPVQRQVESEISFIARGKAITMPMSQLVQEKSYVPSIPRIGRGKKPPPPPPPAGAPAAAIPEERSRFGKMIPNKFSREKAERPPSPPAPPTSRPTIGGARNFSSNHEKVESSQHLSSRLPNPAIGGVRKSSGGRDGGIRSRVPSLNPPATRDESPPPPLPGPRPGQPGSRQSPAGEVQPHDFRSVIKSRSATPLAGIAQNSLSPSQRSDLVRIRSAGSSDGRQPEERLLGAVSPSAIRAAERSLSMGASGSQNSRPAVPRSLTAASILSHKTTVSDASSLSSIAGKKKPPPPPPPKKKGLGKGETWVRALFPFEGQDNGDLVFNEGDRIKVLKKTESTDDWWEGEVHGRKGQFPANYTEIITS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4V4</accession>
    <name>D5G4V4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00000106001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 32 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429992">
        <property type="protein sequence ID" value="CAZ79547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835390.1">
        <property type="nucleotide sequence ID" value="XM_002835344.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4V4"/>
    <dbReference type="GeneID" id="9183029"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000106001"/>
    <dbReference type="eggNOG" id="ENOG502RH41">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021541_0_0_1"/>
    <dbReference type="InParanoid" id="D5G4V4"/>
    <dbReference type="OMA" id="VYWTGPA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901135">
        <property type="term" value="P:carbohydrate derivative metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007577">
        <property type="entry name" value="GlycoTrfase_DXD_sugar-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04488">
        <property type="entry name" value="Gly_transf_sug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ79547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="590" mass="67072" checksum="B895C86E6987392D" modified="2010-06-15" version="1">MDRRKPSSCSRFILLLRNLFIAALAYTFVVEVLHIYQTGTISPFSKGSPKKSTGRDSKGGSLSGGVNWRLRPTTLVEGTNDAFMGRRPDVDTYANYTKLIETCRGSLNGLERMRNVYDCLDYLTRREDDYFFMAENRPSAADPNYKSIEGAAPPSTHYIGDCPGPVQLYHTYWTGPATWRVELFVKAYLYTQNIPCSRLYIWLESDGFPDAVSDMMKDPLFAKFLPLVERGDLVLKAWKFPRRIPLPKGAIAEDPAYTKSLAGKGSLRGTSGEARSPKTEVYIADGVVRMEDGSEWLVLTERQMTFLPVAVSDAVRFIVLHLHGGVYMDMDVMLLRDMRPLLLTPDHNFAERWAVHSHPGDYNTAVMSLNANSSLSSYLVRGGIRMGLNFHPRIIGRMAWKDGRNEEFAMFETGLFDPIWGEFNWGREGRCTVPCFKDYGVAFQGTRGAIKDEWESYDGKPLSERVYQRELELRSRVSDSPPSSADEIERASVEALAMEKGDADVTYGGKPYTLKEDKYPPNNRTLENFFRGAWSYHIHNQWTKHPQPNSWFDIAQKAHDGFLQGTRKNVYGESWTGPDIPAYDRWPEFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5B3</accession>
    <name>D5G5B3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00004256001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835549.1">
        <property type="nucleotide sequence ID" value="XM_002835503.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5B3"/>
    <dbReference type="STRING" id="656061.D5G5B3"/>
    <dbReference type="GeneID" id="9188125"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004256001"/>
    <dbReference type="eggNOG" id="KOG1209">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_9_1"/>
    <dbReference type="InParanoid" id="D5G5B3"/>
    <dbReference type="OMA" id="GGTPDWF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000140">
        <property type="term" value="F:acylglycerone-phosphate reductase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019433">
        <property type="term" value="P:triglyceride catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44169">
        <property type="entry name" value="NADPH-DEPENDENT 1-ACYLDIHYDROXYACETONE PHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44169:SF6">
        <property type="entry name" value="NADPH-DEPENDENT 1-ACYLDIHYDROXYACETONE PHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ79706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="251" mass="28017" checksum="C32548732711BC1B" modified="2010-06-15" version="1">MADIRKTVLITGAGVAGIGGSLAKEFHLRGYRVFATARDLTTVQELSEKYSIETLYLDVTSTESVRAAAEEMSRRAGGKLDVLVNNAGLGVVTPATDLLIETVVKSMFEINVFGVMRMVQEFSGLLIAAEAFQLMKASQIVTGGVKTNIARHGTTLRPGSIYTPILDFFHKRVTDSQDGAMDRDAYARSVVAQVMKPDWRKPAWFWEGNKAKVVWFIWTFLWSTAFDFRMMRVFGLFKLKRIVSAEKSKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5C7</accession>
    <name>D5G5C7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00004271001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835563.1">
        <property type="nucleotide sequence ID" value="XM_002835517.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5C7"/>
    <dbReference type="STRING" id="656061.D5G5C7"/>
    <dbReference type="GeneID" id="9188139"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004271001"/>
    <dbReference type="eggNOG" id="ENOG502QRGK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045411_1_0_1"/>
    <dbReference type="InParanoid" id="D5G5C7"/>
    <dbReference type="OMA" id="HISGYQE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48420">
        <property type="entry name" value="NON-HAEM DIOXYGENASE N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48420:SF1">
        <property type="entry name" value="NON-HAEM DIOXYGENASE N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ79720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="300" mass="32827" checksum="97D71716AA66C4AF" modified="2010-06-15" version="1">MTYQKADPVLVRLDDLRSGNVDLKRLEKAFGPESLGIIVVSGLPEKFIGLRQRLLSFASHLGNLSQLDGSHAEKRSYQMIKEGLLVGFKETFEEFCVMVIDIAGLVARACDRYALKHISGYQEGYLEHVVKTSTSTKARLLHYFPPPALETGSIETEEDLDNWCGTHLDHGCLTGLTSAMYVDEGSLTLDISRGVDLPELESAPDPDAGLYIKDRKGGVAKVGIPRDCLAFQTGEALEVITKGKLKAVPHFVRGCRAGKAAGVSRNTIAVFTQPNLWEKVDGDRDFATFAKEIVAKNTEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5M4</accession>
    <name>D5G5M4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00001487001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for the assembly of cytochrome c oxidase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430000">
        <property type="protein sequence ID" value="CAZ79817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835660.1">
        <property type="nucleotide sequence ID" value="XM_002835614.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5M4"/>
    <dbReference type="STRING" id="656061.D5G5M4"/>
    <dbReference type="GeneID" id="9183803"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001487001"/>
    <dbReference type="HOGENOM" id="CLU_2924392_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5M4"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033108">
        <property type="term" value="P:mitochondrial respiratory chain complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051040">
        <property type="entry name" value="COX23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009069">
        <property type="entry name" value="Cys_alpha_HP_mot_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46811">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46811:SF1">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47072">
        <property type="entry name" value="Cysteine alpha-hairpin motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ79817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="61" mass="7229" checksum="4030DE01477F2468" modified="2010-06-15" version="1">MANHPRTIRKSSSQYFDPCQEAANRSIRCLNRNAGDRTMCSDYFQWVSIFWNGARVADWLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>D5G620</accession>
    <name>D5G620_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Malate dehydrogenase</fullName>
            <ecNumber evidence="3 12">1.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">GSTUM_00001731001</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 12">
            <text>(S)-malate + NAD(+) = oxaloacetate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21432"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.37"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the LDH/MDH superfamily. MDH type 1 family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.37" evidence="3 12"/>
    <dbReference type="EMBL" id="FN430007">
        <property type="protein sequence ID" value="CAZ79963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835806.1">
        <property type="nucleotide sequence ID" value="XM_002835760.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G620"/>
    <dbReference type="FunCoup" id="D5G620">
        <property type="interactions" value="781"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G620"/>
    <dbReference type="GeneID" id="9187641"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001731001"/>
    <dbReference type="eggNOG" id="KOG1494">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047181_1_0_1"/>
    <dbReference type="InParanoid" id="D5G620"/>
    <dbReference type="OMA" id="ASCAEYI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030060">
        <property type="term" value="F:L-malate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006108">
        <property type="term" value="P:malate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01337">
        <property type="entry name" value="MDH_glyoxysomal_mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000013">
        <property type="entry name" value="Malate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.110.10:FF:000001">
        <property type="entry name" value="Malate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="entry name" value="Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001557">
        <property type="entry name" value="L-lactate/malate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001252">
        <property type="entry name" value="Malate_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010097">
        <property type="entry name" value="Malate_DH_type1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01772">
        <property type="entry name" value="MDH_euk_gproteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11540">
        <property type="entry name" value="MALATE AND LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11540:SF73">
        <property type="entry name" value="MALATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00056">
        <property type="entry name" value="Ldh_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000102">
        <property type="entry name" value="Lac_mal_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="LDH C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00068">
        <property type="entry name" value="MDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10 12" id="KW-0520">NAD</keyword>
    <keyword evidence="5 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Lactate/malate dehydrogenase N-terminal" evidence="13">
        <location>
            <begin position="25"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Lactate/malate dehydrogenase C-terminal" evidence="14">
        <location>
            <begin position="170"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="200"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="30"/>
            <end position="36"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="140"/>
            <end position="142"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAZ79963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="347" mass="36683" checksum="8233CE3DE6F20721" modified="2010-06-15" version="1">MFTARSAFSAIGRRAFSVSAQQNSKVTVLGAAGGIGQPLSLLLKLNPRVSELALYDIRGGPGVAADISHVNTKSKVTGYDAVPEQLKAALEGADIVLIPAGVPRKPGMTRDDLFKTNASIVRDLAKAAANHAPNAKLLIISNPVNSTVPITAEVFKSKGVYNPKRLFGVTTLDVVRASKFISEIQGTDPANEEVTVIGGHSGVTIVPLLSQSNHPSIDGETREALVKRIQFGGDEVVEAKGGAGSATLSMAFAGSRMADSLLRASYGETGIFEPAFVQSPLYKDDGCEFFSSRIELGPEGVKEIHPVGKVSKYEEGLIATALKDLATNIKKAWHLVPHSFMPLLTPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>D5G628</accession>
    <name>D5G628_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00001744001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430007">
        <property type="protein sequence ID" value="CAZ79971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835814.1">
        <property type="nucleotide sequence ID" value="XM_002835768.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G628"/>
    <dbReference type="STRING" id="656061.D5G628"/>
    <dbReference type="GeneID" id="9187650"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001744001"/>
    <dbReference type="HOGENOM" id="CLU_1866591_0_0_1"/>
    <dbReference type="InParanoid" id="D5G628"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24987">
        <property type="entry name" value="HEAT_EF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ79971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="137" mass="15001" checksum="61A1F31D4FD11F4C" modified="2010-06-15" version="1">MAPALDTMTPSDAVVASENQKSVKVLDELLQKLSVSKTKADATYAAENLATFINGPIEEHEVPTKILNLIKKQLANKKDALAREHALTAIAAICLKQVSPKCRRCCFPEDCWHDQPQRCQGCSSCHLSLNHQGSEMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5G641</accession>
    <name>D5G641_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00001758001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430007">
        <property type="protein sequence ID" value="CAZ79984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835827.1">
        <property type="nucleotide sequence ID" value="XM_002835781.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G641"/>
    <dbReference type="GeneID" id="9187663"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001758001"/>
    <dbReference type="HOGENOM" id="CLU_2265671_0_0_1"/>
    <dbReference type="InParanoid" id="D5G641"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="103" mass="12152" checksum="FFC29B133C9768A7" modified="2010-06-15" version="1">MIPVWYSTRALYISCAAAFRLRICNPKVLKGGKITLAQLLTITILIYLLIYHTREGKKKKRMRPCFSTPQYKKRREFFFFCEFKNSSPLDKRAQKKKKIAIST</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5G674</accession>
    <name>D5G674_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed RNA polymerase</fullName>
            <ecNumber evidence="2">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00001642001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="2"/>
    <dbReference type="EMBL" id="FN430008">
        <property type="protein sequence ID" value="CAZ80017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835860.1">
        <property type="nucleotide sequence ID" value="XM_002835814.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G674"/>
    <dbReference type="FunCoup" id="D5G674">
        <property type="interactions" value="1174"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G674"/>
    <dbReference type="GeneID" id="9187696"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001642001"/>
    <dbReference type="eggNOG" id="KOG0214">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000524_5_3_1"/>
    <dbReference type="InParanoid" id="D5G674"/>
    <dbReference type="OMA" id="CSTNPNI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032549">
        <property type="term" value="F:ribonucleoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1100.10:FF:000005">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1110.10:FF:000003">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1070.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1110.10">
        <property type="entry name" value="RNA polymerase Rpb2, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007644">
        <property type="entry name" value="RNA_pol_bsu_protrusion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007642">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037034">
        <property type="entry name" value="RNA_pol_Rpb2_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007645">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007646">
        <property type="entry name" value="RNA_pol_Rpb2_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007647">
        <property type="entry name" value="RNA_pol_Rpb2_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04563">
        <property type="entry name" value="RNA_pol_Rpb2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04561">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04565">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04566">
        <property type="entry name" value="RNA_pol_Rpb2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04567">
        <property type="entry name" value="RNA_pol_Rpb2_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RNA polymerase beta subunit protrusion" evidence="9">
        <location>
            <begin position="3"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="8">
        <location>
            <begin position="139"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="10">
        <location>
            <begin position="402"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="11">
        <location>
            <begin position="501"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="12">
        <location>
            <begin position="556"/>
            <end position="592"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF04567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ80017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="620" mass="70184" checksum="537177C450765248" modified="2010-06-15" version="1">MVEMIDEDSLLTLDQNTPQSDDGIVLRRYEIMFGPCYLSRPTMTEGDGSTQPMLPQEARLRNLTYSAPMYCELKKKVMARVRYEWDIEEEDEGATKVFIGKMPVMLKSHLNECPYDQGGYFVINGSEKVLIAQERSAANIVQVFKKALPSPTPYVAEIRSAVEKGSRLISSMQIKLFSKGEARGGYNATIKSTLPYIKSDIPIVIVFRALGVVSDEDILNHVCYDRNDTQMLEMMKPCIEEAFVIQDREVALDFIGKRGSSQGINKEKRIRYARDIMQKELLPHISQEEGSETRKAFFLGYMVHRMLQCALERREVDDRDHFGKKRLDLAGPLLAGLFRTLWRKLTKDVYKYLEKCVETSKDFNLTLAVKSNTITNGLKYSLATGNWGEQKKAASSKAGVSQVLNRYTFASTLSHLRRTNTPIGRDGKIAKPRQLHNTHWGLVCPAETPEGQACGLVKNLSLMSYITIGTPGYPLVDFLSSNDMELLEEYEPQRSPNATKVFVNGVWVGTHRDPLMLAKVVQDVRRQGVVSHEVSIFTDAGRVCRPLFVIDNDPRKEEEGVMIVMTPEDLDLSRQAPINSTTHTWTHCEIHPILFRSPTIISHPVILINPQWVSKPWVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6B7</accession>
    <name>D5G6B7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00004411001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. ADCK protein kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430009">
        <property type="protein sequence ID" value="CAZ80060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835903.1">
        <property type="nucleotide sequence ID" value="XM_002835857.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6B7"/>
    <dbReference type="FunCoup" id="D5G6B7">
        <property type="interactions" value="88"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G6B7"/>
    <dbReference type="GeneID" id="9187431"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004411001"/>
    <dbReference type="eggNOG" id="KOG1236">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006533_6_0_1"/>
    <dbReference type="InParanoid" id="D5G6B7"/>
    <dbReference type="OMA" id="SMVRTHH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13971">
        <property type="entry name" value="ADCK2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044095">
        <property type="entry name" value="ADCK2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052402">
        <property type="entry name" value="ADCK_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45890:SF1">
        <property type="entry name" value="AARF DOMAIN CONTAINING KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45890">
        <property type="entry name" value="AARF DOMAIN CONTAINING KINASE 2 (PREDICTED)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC1 atypical kinase-like" evidence="2">
        <location>
            <begin position="220"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="ABC1 atypical kinase-like" evidence="2">
        <location>
            <begin position="310"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ80060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="725" mass="82333" checksum="C9A82AD8E4951011" modified="2010-06-15" version="1">MGVMALSCRRLSTIPFLRSPPKPIFTIRHTLSRSSYRSPFTTYSNPFPNQPIRWPRPNRNYTLWIGSASLAFALHLGLSPPLTLETGATGSEKTAEQLMLEESERERLEPCRALSGQPLILRILKRAQLLAIKCVIEPIATGLRFVHLVVIFLPVILSIPLACIGPRQPDRCSERIGTIWWYSFLIKSMERAGPTFIKLGQWAASRTDIFPAEMCEMMSKLHSDARAHSLRDTKRTISQAFGGRHFDEIFEEFDQKPLGIGAVAQVYRAKLSPHLLPRLHELGETDFKKNLRRKVDVLVKKAPYQGVPSSYVAVKVLHPKVDRTVHRDLRIIYFFASLLNLVPTLEWLSLPDEVDKFSEMMRLQLDLRIEANNLSRFRSNFGDRTTVTFPLPYSNYTTREVLIEEFAHGVPLSFFLESGAGPFRKEMADMGLDAFLHMLIMDNFIHADLHPGNIMVRFYKLEPLPSLSQFPLVVPGRKASARGADNHKEVTEEVISRLIPHRGNPQEWQKELNLLDNEGYRPQLIFIDTGLVTELNSVNRRNFLDLFRAIAEFDGYRVGHLMIERCRSPSAVIDNEIFTLKMQHLILAVKSRTLALGNIKIGDLLTQVLQMVRVHHVRMEGDFINVVLSILLLEGIGRSLDPNLDLLKRYTVLGRYFELLADICSSLPMLRSIGAGSGAQMLGKRGKSDLGLLKVWIALEARQFINSSVEDVENLVKYDLLSPNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6F4</accession>
    <name>D5G6F4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00004458001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FGGY kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430009">
        <property type="protein sequence ID" value="CAZ80097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835940.1">
        <property type="nucleotide sequence ID" value="XM_002835894.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6F4"/>
    <dbReference type="FunCoup" id="D5G6F4">
        <property type="interactions" value="279"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G6F4"/>
    <dbReference type="GeneID" id="9181182"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004458001"/>
    <dbReference type="eggNOG" id="KOG2517">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009281_10_1_1"/>
    <dbReference type="InParanoid" id="D5G6F4"/>
    <dbReference type="OMA" id="HKAMWHE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019150">
        <property type="term" value="F:D-ribulokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019321">
        <property type="term" value="P:pentose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07782">
        <property type="entry name" value="ASKHA_NBD_FGGY_D-RBK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000577">
        <property type="entry name" value="Carb_kinase_FGGY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018485">
        <property type="entry name" value="FGGY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018484">
        <property type="entry name" value="FGGY_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006003">
        <property type="entry name" value="FGGY_RbtK-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01315">
        <property type="entry name" value="5C_CHO_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43435:SF4">
        <property type="entry name" value="FGGY CARBOHYDRATE KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43435">
        <property type="entry name" value="RIBULOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02782">
        <property type="entry name" value="FGGY_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00370">
        <property type="entry name" value="FGGY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000538">
        <property type="entry name" value="GlpK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase FGGY N-terminal" evidence="4">
        <location>
            <begin position="12"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate kinase FGGY C-terminal" evidence="5">
        <location>
            <begin position="292"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ80097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="571" mass="61835" checksum="75EB9123029E301D" modified="2010-06-15" version="1">MATTMGTSNDHFLGVDVGTGSVRVCIIDGTGEIKGLESKDIKTWHEKADYYEQSTEDIWSAICYCSRLVLEKSGLDGASVKGVGFDATCSLAMGRGMLFVCVLLRPEKETKAVNATNHKLLQYVGGSMSIEMEIPKVLWLKNNMPKELFNKCKFYDLADVLTHMATGKETRSYCSAVCKQGFVPVGVDGSVKGWQEDFLNDIGLPELAENGFMRMGGVHGASGTMLSAGELVGGLSEKAAKELGLVAGIKIGSGVIDAYAGWIGTVGAKMEMPQNLLSGGHAGSLEQAFRRLAAVAGTSTCHLVMSRDPIFVPGVWGPYRDVLLPGYWMAEGGQSSTGSLLHNVLTTHPSYNDALEKSNELSKNIFEYLNEHLETLRKSECAPAIGYLARHLFFYGDKHGNRSPIADAAMRGAIVGLSMDSSINDLALQYFAAMEFIAQQTRHIIDTLNSRGHEITSIFMSGGQCKNDILMHLIADATGYPVVIPRYIDAAVVLGAAMLGAKAASVDSEGNTESLWAIMDRMSKPGRTIYPTENANERKLLGAKYEVFLHMSKAQQTYRDMVNAAIGDWKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6S3</accession>
    <name>D5G6S3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00002245001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430014">
        <property type="protein sequence ID" value="CAZ80216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836059.1">
        <property type="nucleotide sequence ID" value="XM_002836013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6S3"/>
    <dbReference type="STRING" id="656061.D5G6S3"/>
    <dbReference type="GeneID" id="9187241"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002245001"/>
    <dbReference type="eggNOG" id="ENOG502S92Z">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_323710_0_0_1"/>
    <dbReference type="InParanoid" id="D5G6S3"/>
    <dbReference type="OMA" id="CVREECC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013882">
        <property type="entry name" value="Ctp1_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08573">
        <property type="entry name" value="SAE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA endonuclease activator Ctp1 C-terminal" evidence="6">
        <location>
            <begin position="753"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="140"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="268"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="339"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="466"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="534"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="682"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="790"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="37"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="268"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="347"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="361"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="583"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="596"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="682"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="808"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="865"/>
            <end position="883"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ80216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="893" mass="98444" checksum="FA917970250787A3" modified="2010-06-15" version="1">MDILGRHRIAFNNLGVQLFADLERDLAPVLTKGLGEKADLERVIAEQKELLQERQALVDAYQSASEQQQQKIREQENRLQSLGYGIAKTEIENEALVERLEEIEKADRERQRRALAITKDKSLENKENIPPVFNYLAQNTPSRTLTRPVSPPPKPLNKPRIPSSTPAGGATRTLFSYKDGEVQTDPIYSLDDLKKAYQELASSYNDIHYELDQYKEAYSRLAEKYAQNKAVWKQWSEQENTRLINSKKRRLEEGTTELRAVGRRINGYTTPATSITGGSPTSRAPNISPIKPRFRPSVVHSSPKNGGLFHELLESGAGMAGEVKGSFELVGGKGLVGGGPKEGSVELPSSPLSSPPSSLSTPETQVPSRTLGATRNSKSRDDGDSTESDDEPRPSNRCNDALESVIPSRDSAFKIPVVKEETGACGRGRISQEGDSMARPVVIKSESHTSSEGLGYHMFDQESLDLDDIGQKPQTPRKRQKPRYPELGASENIGRLGWISGGFRIAPTGDTGEAQDAENDLPVPIFPPSAVGEGKPVGIWPDSQLVRPTTPVQTAGAGPRSSFPISAENEGSKTHPPLRRSSRTSATRKTLSPTTKAKKLNAHSPPKAKKRRMATSRAIASLAEDGTDGCEDINTSSIHPPMVLSEKQRKQLERDMKGTTRLDDILNSPASRTPNLARLKKSIQSRTSPVRKRTWGGDGFGAPQVRPPSSAGPSTSAWRAGGNMKSKARDISSTLDISDFRVNPEVNDGKDYAFAETVRNRDARKCLPGCMKTCCRDLVGFTEAAGLPAVSKGPRWRSSSPAPVDGCDLPRRNPRKESVSREDKAKEFTNRYGRHRDAFERRRSPPGFWDADFPTTPELEQQRVVAEEMRRKKVEDMRKEAGKGNKGRYVYRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6X0</accession>
    <name>D5G6X0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NADH dehydrogenase [ubiquinone] iron-sulfur protein 5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Complex I-15 kDa</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">NADH-ubiquinone oxidoreductase 15 kDa subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">GSTUM_00002365001</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Mammalian complex I is composed of 45 different subunits. This is a component of the iron-sulfur (IP) fragment of the enzyme.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion inner membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the complex I NDUFS5 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430019">
        <property type="protein sequence ID" value="CAZ80263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842528.1">
        <property type="nucleotide sequence ID" value="XM_002842482.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6X0"/>
    <dbReference type="STRING" id="656061.D5G6X0"/>
    <dbReference type="GeneID" id="9184077"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002365001"/>
    <dbReference type="HOGENOM" id="CLU_162182_0_0_1"/>
    <dbReference type="InParanoid" id="D5G6X0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd24141">
        <property type="entry name" value="NDUFS5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019342">
        <property type="entry name" value="NADH_UbQ_OxRdtase_FeS-su5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15224">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15224:SF1">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="75"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="75"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="15"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="25"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR619342-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAZ80263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="99" mass="11537" checksum="A3C0FB678FE1020C" modified="2010-06-15" version="1">MPYSLGAHADIMFKCFPFWQDLLSCYVVNTGSATSDEGKWKCVPQRDDYYECLHHKKEIRKTQAIKAAYNRRLKQDPNFDPRKPEGEADIRSLGLLQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6Y4</accession>
    <name>D5G6Y4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00002383001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430019">
        <property type="protein sequence ID" value="CAZ80277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842542.1">
        <property type="nucleotide sequence ID" value="XM_002842496.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6Y4"/>
    <dbReference type="GeneID" id="9184091"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002383001"/>
    <dbReference type="HOGENOM" id="CLU_3368792_0_0_1"/>
    <dbReference type="InParanoid" id="D5G6Y4"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ80277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="35" mass="4148" checksum="AC4F77AE9222F369" modified="2010-06-15" version="1">MATTPMSRKKKPKQVSDHTKFNVLLHKLYNNKLYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7T4</accession>
    <name>D5G7T4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00004715001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430031">
        <property type="protein sequence ID" value="CAZ80577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836386.1">
        <property type="nucleotide sequence ID" value="XM_002836340.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7T4"/>
    <dbReference type="STRING" id="656061.D5G7T4"/>
    <dbReference type="GeneID" id="9181396"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004715001"/>
    <dbReference type="eggNOG" id="ENOG502S8RT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_183941_2_0_1"/>
    <dbReference type="InParanoid" id="D5G7T4"/>
    <dbReference type="OMA" id="RYKYWRW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39476:SF1">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 BETA SUBCOMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39476">
        <property type="entry name" value="NADH:UBIQUINONE OXIDOREDUCTASE 6.6KD SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="76" mass="8927" checksum="B9D6AF935EAC5271" modified="2010-06-15" version="1">MAGGHYREVLHQDPALIKYANLNTNRYKYFRWNARTAKVSFIYAVVIPAALLTLAYKTEGKYNFRDKRRGDIPQDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7U4</accession>
    <name>D5G7U4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Palmitoyltransferase</fullName>
            <ecNumber evidence="11">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00002655001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DHHC palmitoyltransferase family. ERF2/ZDHHC9 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="11"/>
    <dbReference type="EMBL" id="FN430032">
        <property type="protein sequence ID" value="CAZ80587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836396.1">
        <property type="nucleotide sequence ID" value="XM_002836350.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7U4"/>
    <dbReference type="STRING" id="656061.D5G7U4"/>
    <dbReference type="GeneID" id="9181406"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002655001"/>
    <dbReference type="eggNOG" id="KOG1311">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021757_0_0_1"/>
    <dbReference type="InParanoid" id="D5G7U4"/>
    <dbReference type="OMA" id="VPVKYCK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF43">
        <property type="entry name" value="PALMITOYLTRANSFERASE APP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="323"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="463"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="506"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="13">
        <location>
            <begin position="418"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="63"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="239"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="588"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="63"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="98"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="126"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="239"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="264"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="605"/>
            <end position="624"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ80587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="624" mass="69054" checksum="4EF80D9C1340F0BD" modified="2010-06-15" version="1">MASPRGSPLSAVFLNSPTHGALPLSLHEINEDQPDGGAYSVISSRMTDVSDNASDIMGLAEAQLSQNASSRPSSLQPGIGRANTRVSVSDPQPDAFSTRPSTAMSTSTAARGWSNPPPSRRGLSSVGGSQRGRPSSSASRTHAPSLTSHAFYRPMSSTKLQAQRGKVTEEDEAEQNRIRGFAQAARGVSDRPPVLGLSMHEPMIHQHLVSDDRAQYVQSGSGSPTGHTMHTAQSITSVSPLNTSAQSSLDNRQSAGNDHRVVMNGSNGNQLPKLDNSSRGSSVAKPKQHSQPYKGKNWQYFPGKTAFCLGGRFQTARDVPMNLLTAILVTLPACLFCAYSAKWLWKHVSPALPVTFGYLYLLCMMSFLKASVSDPGVYPRNVHPLEVDDADDALAVPPPNGWASIKPLKHQVHLEVPIKYCRTCRIWRPPRCHHCRICDNCIETQDHHCVWLNNCVGRRNYRYFFVFVSTATLLGFYLLALSLVHLNEWKKQTSHSFSDAIREWQVPFGMVIYGALAAPYPLALLGYHIFLMARGETTREYLHGHKFVRSERHRPFSQINAFYNFVVVLCRPRPPTYVELKSNYQTGDQRFERQQQQRLSTNKSEAGDRADESAPVVERRISAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7X6</accession>
    <name>D5G7X6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00002622001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430033">
        <property type="protein sequence ID" value="CAZ80619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836428.1">
        <property type="nucleotide sequence ID" value="XM_002836382.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7X6"/>
    <dbReference type="GeneID" id="9181438"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002622001"/>
    <dbReference type="HOGENOM" id="CLU_924992_0_0_1"/>
    <dbReference type="InParanoid" id="D5G7X6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="301" mass="33769" checksum="F2C0D699BF235C03" modified="2010-06-15" version="1">MSATCTPRSCIPAFSPTQLLPASVLCGPSCSLGRGFWLALAVTVIVIVIIVIAMVIAIRMVLVLVLILVLVLVRLIFIVRLIFLIIIWASWTIPVFAWPIWICPRGWSTGFVGTGSMPPEKHVHRFAWRTLSPPHADMQVINENDDFRQRQGFLGGVEKQKAAVPGGAQEMTVAHGARQKAVNIREEVFERRLSQLHPENSFMGLPLDPVETAGERGVCTVSGWSIKPKPDYTRTSQTYVYDISTLTSTIMRTVRVGTYYHWKQPVRAEEQEQPEGQGYPLPSTFWNAFLQYHSIKSRNIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7Z2</accession>
    <name>D5G7Z2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00002739001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430035">
        <property type="protein sequence ID" value="CAZ80635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836444.1">
        <property type="nucleotide sequence ID" value="XM_002836398.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7Z2"/>
    <dbReference type="FunCoup" id="D5G7Z2">
        <property type="interactions" value="371"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G7Z2"/>
    <dbReference type="GeneID" id="9184108"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002739001"/>
    <dbReference type="eggNOG" id="KOG0101">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005965_0_3_1"/>
    <dbReference type="InParanoid" id="D5G7Z2"/>
    <dbReference type="OMA" id="DQVLMDH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10232">
        <property type="entry name" value="ASKHA_NBD_HSP70_ScSsz1p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000010">
        <property type="entry name" value="heat shock 70 kDa protein 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000009">
        <property type="entry name" value="Heat shock protein Hsp70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639:SF32">
        <property type="entry name" value="HEAT SHOCK PROTEIN PDR13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639">
        <property type="entry name" value="HSC70CB, ISOFORM G-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ80635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="542" mass="57689" checksum="2287217CC433B22E" modified="2010-06-15" version="1">MSGNASPSPPASPEIDDGTKTVIGIAFGNTTSSIAYTTPDGKAEVIANEEGDRQIPSVLSWVGSEEYHGTQAKSHLIRNAKNTVAYFRDFLGKKYSEIDPTNSHNSAHPLDVDGVPSFNLQQADDETVSTVSINEITTRHLIRLRDSASDFLGRKVTGAVITVPTDFSDTQKEALVAAAKAGELEVLQIIHEPVAAVLAYAARRTPEAQGVDRNILVADLGGTRCDAAVVASRGGMYTILATAHDYGLGGLRLDEVLVNHFSKEFIKKHKVDPGNERSLAKLRLEAEGTKRTLSLGTSATISIESLADGYDFHSTINRLRYELSAKKVFDQVVSLVENVVKKAELDLLDIGEVILSGGTSHTPKIASRIAALFPDSVPVNAPATLTTALNPSELSARGAAIQASLIAEFDKEDIEQSTHPAVTVAPHLSHPIGVVIGEDNSFQTILEAQTAVPARRIAQFDVQADGDVIVRICEGIREIVVEKVERQVNVIAEAAIRGAKRGGKVEVTINVGSDLGLNVAARVIGTQIGVRGEVKPEAGSAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5G806</accession>
    <name>D5G806_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00002772001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430037">
        <property type="protein sequence ID" value="CAZ80649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836458.1">
        <property type="nucleotide sequence ID" value="XM_002836412.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G806"/>
    <dbReference type="FunCoup" id="D5G806">
        <property type="interactions" value="48"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G806"/>
    <dbReference type="GeneID" id="9184122"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002772001"/>
    <dbReference type="eggNOG" id="KOG3637">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011756_0_0_1"/>
    <dbReference type="InParanoid" id="D5G806"/>
    <dbReference type="OMA" id="ADATWHA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004621">
        <property type="term" value="F:glycosylphosphatidylinositol phospholipase D activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.130">
        <property type="entry name" value="Integrin alpha, N-terminal"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001028">
        <property type="entry name" value="Gprt_PLipase_D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029002">
        <property type="entry name" value="PLPC/GPLD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23221">
        <property type="entry name" value="GLYCOSYLPHOSPHATIDYLINOSITOL PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23221:SF7">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN-SPECIFIC PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00882">
        <property type="entry name" value="Zn_dep_PLPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00718">
        <property type="entry name" value="PHPHLIPASED"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69318">
        <property type="entry name" value="Integrin alpha N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072009" evidence="1">
        <location>
            <begin position="17"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipase C/D" evidence="2">
        <location>
            <begin position="49"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ80649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="855" mass="92438" checksum="314D1042F68D4569" modified="2010-06-15" version="1">MMRVLAGLSLLGLASACGVLVHNEVFQRSADVFALPTSNEDISTHQGVFSRLLKIHTESVQAGAFFPDWGYQCLNTNDDAEAAHWPPFLIASVEHINSRYGSLTSKQRTPEEQEHLEKLIGFVFAVASHQVADATWHAIRLPTAFLSALAGVDFDGDEAAAHRVLDFGGDVVLSSRIARNGEDGKVWIGDRWWVPVEDLVAIYSRIGRDVSSFVMRYCTMRGLAALKSRYDTVASDSPMLTAELDGYYLGGINEMAARSVTCWANLTKWIAFGIDKEEKLMNGWGVCDVFRAIQARGGAGSSLHGEYRERKMTGKRMRKYPGGITFTKDRFGAESYHIPGMHPPSVEKPMSLKLSQDAPKLRDPIYTSAYQFYSHFGASLSIGEFSPFDDGQAIAIGAPWESTDSSKPGEGNVYILPLKDFAKPTLQSVPKPLPLVKQPTASTDQRFGISTTPWRIMNTTFLAVAAAGPLTYDPSTPPSLPFQGTSPAGKIHVFPKGSGSSVFSITFKGAELGSIGRRWWGETMLSADLAGDGEEKLIVSGSRSDGLRICEGRGKVQFGEGEIAVISPSRSSSSPSSSDQPIHVELSEGQDILLNATILSLPESAKATPCSKTSTYENFGAAIAFSAHSRTLFISAPGTSSVFAYRWNPISSSLKHVYTIPAPRAPRPFLPSSSSSRGNFGATVTAGYTSGIEWLAISATNEDVDGVKQSGVLRLYTLSQTGAELVAEVAPEPTGAEWARFGKALAKDGDSGLWIGSEFWDAGRGAVWWLHVGRVLDGERVVARAQVVLKEQEEAVKKISLKPVRVGQEANARFATSIASGANRELIVGIPRAGVTHPLQSERFFGYVAFFGRVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8F8</accession>
    <name>D5G8F8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00004806001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430042">
        <property type="protein sequence ID" value="CAZ80801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836610.1">
        <property type="nucleotide sequence ID" value="XM_002836564.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8F8"/>
    <dbReference type="GeneID" id="9186021"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004806001"/>
    <dbReference type="HOGENOM" id="CLU_344898_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8F8"/>
    <dbReference type="OMA" id="SEMNAPE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="344"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="646"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="186"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="219"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="344"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="374"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="409"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="429"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="489"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="515"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="668"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="691"/>
            <end position="701"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="820" mass="88820" checksum="07024106E46B2382" modified="2010-06-15" version="1">MSHSPPHSRTPPTSVSPPRRLTWRRAATSPPRRAIDYSPSRGPTNALYSYPSSSSLASLYLAHGRISPVRRSPAHSRCSSRDQDGVVLCDELRTLAAVIPRKISSLSTTPIPTGGPIPPPTPPPASPHDDIDDLESSGGSDEEIVFQRGRRRPIPNTDIDGLTTPQSRHVRSSRVLRRVRANIHEVSPSGSGSVSPPTVPAGVSAPPTPPKDAAPSGLHHPKSLKRSPKSAYFADSTEVPGLKEHLGREEQNSLDLEEFVWKDEVDHTFAPYIPRTAPAVSGDFLEPKEVEEEQVSSVGELEGSLEENVEIHEALYAEFAMAHEIGIALGSPPAVERRLEFVLDPEHDTSGDDKSVNSNKSVDSIKSEMNAPEPEPESEPESEYESEPEHSIPGAFKDEDEVLAEINGLKKKGRGKGKAKAEVVAITQEEIDSGESTKEEEDENESETGQYLPLGATIAKARINGTGGRKVTPWPTPRIISCRSSMENAQRETADPSESVREPAEEVAEKAASEEAAETESADTDENDFTDEEEDDDEVFYDAEEGGLASPPMSPISRSYSHSRHAELENLPRTPRSPKSPKSPRENNVDLVLTQSASEASAPGLGPGSPNSPKHPEAPKKLPLPLPGPLAVNVIPATPVPLMSPAAELDKQLGNPPPSLLHRKPTGGVQTLKNSHDPTSPTKPSIPVPKRSTSFSMSGTRKTARRPSISNQPKRVRESKLHPWWRPRHGGSNDAEDPGLSRTISAPHISAYTQELHRTESTTSIGRKRVTKPIKGTKFQIEFIGVGTIKEKGGVLKEKLDAGSGAMKRKFSLKKRPTDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8F9</accession>
    <name>D5G8F9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00004807001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M67A family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430042">
        <property type="protein sequence ID" value="CAZ80802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836611.1">
        <property type="nucleotide sequence ID" value="XM_002836565.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8F9"/>
    <dbReference type="FunCoup" id="D5G8F9">
        <property type="interactions" value="1269"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G8F9"/>
    <dbReference type="MEROPS" id="M67.A10"/>
    <dbReference type="GeneID" id="9186022"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004807001"/>
    <dbReference type="eggNOG" id="KOG1555">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052991_0_0_1"/>
    <dbReference type="InParanoid" id="D5G8F9"/>
    <dbReference type="OMA" id="KTGRHEM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034515">
        <property type="term" value="C:proteasome storage granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140492">
        <property type="term" value="F:metal-dependent deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000266">
        <property type="term" value="P:mitochondrial fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032436">
        <property type="term" value="P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902906">
        <property type="term" value="P:proteasome storage granule assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd08069">
        <property type="entry name" value="MPN_RPN11_CSN5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000001">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050242">
        <property type="entry name" value="JAMM_MPN+_peptidase_M67A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056263">
        <property type="entry name" value="RPN11_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10410">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01398">
        <property type="entry name" value="JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23594">
        <property type="entry name" value="RPN11_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102712">
        <property type="entry name" value="JAB1/MPN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-0647">Proteasome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MPN" evidence="8">
        <location>
            <begin position="34"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ80802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="331" mass="36987" checksum="F3B96ABD51300712" modified="2010-06-15" version="1">MDRLNQMYAAAQGLGGMSAPPGVDTPVIDNSETVYISSLALLKMLRHGRAGVPMEVMGLMLGEFVDDYTVRVVDVFAMPQSGTGVSVEAVDPVFQTKMMDMLRQTGRPETVVGWYHSHPGFGCWLSSVDINTQQSFEQLTPRAVAVVIDPIQSVKGKVVIDAFRLINPQSLMLGQEPRQTTSNLGHLNKPSIQALIHGLNRHYYSILINYRKTHLEENMLMNLHKTVWTDGLTMKDFKLLAKENQERLEKMVGLADSYEKRVKEENDLTKDDMKTRYVGKVDPKKHLEELGKRGIEENIVGVLVEMVDKEASFAADIDGSKPGHVEAMDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8I4</accession>
    <name>D5G8I4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="11">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00002887001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="8">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M20A family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430046">
        <property type="protein sequence ID" value="CAZ80831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836640.1">
        <property type="nucleotide sequence ID" value="XM_002836594.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8I4"/>
    <dbReference type="FunCoup" id="D5G8I4">
        <property type="interactions" value="337"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G8I4"/>
    <dbReference type="MEROPS" id="M20.017"/>
    <dbReference type="GeneID" id="9186051"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002887001"/>
    <dbReference type="eggNOG" id="KOG2276">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029469_3_0_1"/>
    <dbReference type="InParanoid" id="D5G8I4"/>
    <dbReference type="OMA" id="CNVKFMI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070573">
        <property type="term" value="F:metallodipeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008242">
        <property type="term" value="F:omega peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006751">
        <property type="term" value="P:glutathione catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05676">
        <property type="entry name" value="M20_dipept_like_CNDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001261">
        <property type="entry name" value="ArgE/DapE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017153">
        <property type="entry name" value="CNDP/DUG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051458">
        <property type="entry name" value="Cyt/Met_Dipeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43270">
        <property type="entry name" value="BETA-ALA-HIS DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43270:SF4">
        <property type="entry name" value="CARNOSINE DIPEPTIDASE 2, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037242">
        <property type="entry name" value="CNDP_dipeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00759">
        <property type="entry name" value="ARGE_DAPE_CPG2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="10">
        <location>
            <begin position="219"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="100"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="166"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="7">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between homodimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between homodimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between homodimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="7">
        <location>
            <position position="343"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between homodimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="7">
        <location>
            <position position="417"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between homodimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="445"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="7">
        <location>
            <position position="445"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between homodimeric partners</note>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="9">
        <location>
            <position position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR037242-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR037242-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR037242-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR037242-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ80831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="476" mass="52449" checksum="46F812F28B8B13ED" modified="2010-06-15" version="1">MAPQLDGYFKQVDLMADSFINRLRSAVAIPSVSADPGLRPKVVAMADFLEKELRALGASVERRLPGKQPGHPDLDLPPILLGRYGNDAKKRTILVYGHYDVQPALKEDGWATEPFDLTVDEQGRMYGRGSTDDKGPVLGWINSIEAHQKAGVDFPVNLLMCFEGMEEYGSEGLDDVIKEEASKFFKDAEAVCISDNYWLGTKKPCLTYGLRGCSYYSLAVSGPGQDLHSGVFGGTVTEPMTDLVRLLATLVGNKGKIHIPGLNELVAPLTDEEKELYKDISFEMSDLYQSLGSETSIYLNKEDTLMARWRFPALSIHGIEGAYSAPGAKTVIPAKVIGKFSIRTVPNMEPDKVDELVFKFVDAEWKKLGSNNTMKCELQHSGNWWVASPKHWNFSAAGKAVEKVFGVKPDFTREGGSIPVTLTFEQATGKNVLLLPMGSSTDAAHSINEKLDKRNYIEGTKLLGAYLHYVAEEEQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8S1</accession>
    <name>D5G8S1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00003044001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid/polyamine transporter 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430052">
        <property type="protein sequence ID" value="CAZ80914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836723.1">
        <property type="nucleotide sequence ID" value="XM_002836677.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8S1"/>
    <dbReference type="FunCoup" id="D5G8S1">
        <property type="interactions" value="512"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G8S1"/>
    <dbReference type="GeneID" id="9187120"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003044001"/>
    <dbReference type="eggNOG" id="KOG1304">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009646_3_1_1"/>
    <dbReference type="InParanoid" id="D5G8S1"/>
    <dbReference type="OMA" id="DTHRHIY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061459">
        <property type="term" value="F:L-arginine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005290">
        <property type="term" value="F:L-histidine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015189">
        <property type="term" value="F:L-lysine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005302">
        <property type="term" value="F:L-tyrosine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990818">
        <property type="term" value="P:L-arginine transmembrane export from vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0089708">
        <property type="term" value="P:L-histidine transmembrane export from vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0089707">
        <property type="term" value="P:L-lysine transmembrane export from vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000067">
        <property type="entry name" value="Transmembrane domain transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF666">
        <property type="entry name" value="VACUOLAR AMINO ACID TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="361"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="406"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="446"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="470"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="512"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="545"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="592"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="634"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="657"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="690"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="8">
        <location>
            <begin position="331"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="150"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="292"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="82"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="114"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="155"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="305"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ80914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="719" mass="78111" checksum="A87BBF9D6C921DD4" modified="2010-06-15" version="1">MPSSPPPESSASSYQQRAPSPAPRPGSPRSTTPALREQATVRLGSPVPSHGFPTASTPRPSSPVIGASNPLAQINWAGSGGTPPRFGTPPRLGTPTRYGTPPPANFGSGFGINIANSSSKPPSIQYGSYDSRQPIEDPEVVRRHLVTEDPSITGLVGGSSSGNRGTGGGLDDEFSSLQLQGGDVTRPIYRYVESIEASGGRRSRSRSFHVPRPEPEDDTLDINSIKQIGGFRRNYIRRNEGASPTGDGHGTQLGRPFLTRNFIEFLTLHGHFAGEDLEDDEAWTSDEALMAEEGRRDEDEEEPGEGSALLAGRARTSGRRRSRSISQGTAGSGKAVLLLLKSFVGTGVLFLPKAYSNGGMLFSNLVLLFIAALSYYCFVLLVRTRLRVAGSFGDIGGILYGEKMRILILSSIIISQIGFAAAYIVFTSENLQAFILAVTNGERLIEIKYLILTQLLVFLPMSMIRDMAKLGGTALIADFFIMLGLIYLYYYDFFTLATEGVSDIVNFNNKDWTLFIGTAIFTFEGIGLIIPIQETMKHPHKFPKVLGGVMVIITIIFVSMGALSYAAYGSGTRTVIILNLPQDDKFVNGVQFLYSLAILLSTPLQLFPAIRIMETGLFPRSGKNNPSVKWQKNVFRFITVFLTALISWGGADDLDKFVALIGSFACIPLVYIYPPLLHMRAHNTSNSMKAADIGLCIFGIVVMAYTTALTIKKWSDPNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>D5G968</accession>
    <name>D5G968_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00003173001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="FN430056">
        <property type="protein sequence ID" value="CAZ81061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836870.1">
        <property type="nucleotide sequence ID" value="XM_002836824.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G968"/>
    <dbReference type="FunCoup" id="D5G968">
        <property type="interactions" value="188"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G968"/>
    <dbReference type="GeneID" id="9184347"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003173001"/>
    <dbReference type="eggNOG" id="KOG0585">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_300563_0_0_1"/>
    <dbReference type="InParanoid" id="D5G968"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042149">
        <property type="term" value="P:cellular response to glucose starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001558">
        <property type="term" value="P:regulation of cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14008">
        <property type="entry name" value="STKc_LKB1_CaMKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000206">
        <property type="entry name" value="Serine/threonine-protein kinase Ssp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF77">
        <property type="entry name" value="SERINE THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="108"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="64"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="525"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="818"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="913"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="11"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="551"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="596"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="823"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="838"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="916"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="979"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ81061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="996" mass="111310" checksum="713E901A6BA55663" modified="2010-06-15" version="1">MECHNNHLVGHSSNNTSTTTSRHASTTSMFSLANLHQPHSRNPTPPTPMSSPGLYSPPHISPSDRTVMGPHLHPSQYRQPEHNTSVRETHLLDLDFDPISGRKIINHYSIIDEIGRGVHGKVKLGTDLDTGELVAIKIVERSQDRPRLGRRGDGESEKKVRREIAILKKCCHENVVRLLEVIDDPQSKKVYLILEYVQLGEIIWRKPGVSYVPSLTIEQARCTFRDTVLGLEYLHYHGIIHRDIKPANLLWTQDRRVKISDFGVSFLGRPIRDYEDGCDGEEATGLEQNELELAKTAGTPAFFAPELCHVDHNQPRPQITSAIDVWALGVTLYCLVFARCPFMADNEYQLLRVIAKDELVIPNRRPIDVDLKDLLKRLLTKDPTRRITLREVKRHPWVLHGIADPCAWVDETDPERMSDGNRIEVTVEDVEKAVSSAGVLNRARSAVKRAVEWGRGLRRRASSTANVISTKDMKILAEKEREVTATRCCPSAHLGDDDGELRWMGSNPQWRDEDKSLLSACSAANNTASSDPVRDGGGYCGSNGESLDPNSPRRSDSESQRLQRKCSSSLCAPSRRHAIAGNEPSGTNAGFHRTHGNLSQGGPTENPHISRSDTSLRAPSYGHHAGSLIGNGVRRVVRSMRSGGFHDNGDKVKGSVSRECSPLRVRDPLAGALRGDHSFYAYGTGSLHVQIPNGRRHSYAGIDVDGSVIDNNRLSAIDCKPRWDREISKKFASFTMKDKSCPTSPDDEVSMEKIWERDQQKAFLRHEERNKYTSPGASNADVTPGWTKESYYHPSHESPTPPIMMERRHRGFFDDHIQMPMNSGQQSHLVSSSSEEQFATTAGSSLTNSTSFPSVPSIVSENSSISSEFFNSRYSRKNSIAGVVHEEEFDMNYRPPTPFHQSRLSDIRQIDSDNEHEMDDDPDDDDDSDGGFIMEVPPPKKSVRSESIAVDKLARRPLDPIEPVPRTRRRSRSSSAALKNKKARRGRTKEKVVTGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9T1</accession>
    <name>D5G9T1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00005059001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430064">
        <property type="protein sequence ID" value="CAZ81274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837083.1">
        <property type="nucleotide sequence ID" value="XM_002837037.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9T1"/>
    <dbReference type="GeneID" id="9188517"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005059001"/>
    <dbReference type="HOGENOM" id="CLU_1344112_0_0_1"/>
    <dbReference type="InParanoid" id="D5G9T1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="47"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="102"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="174"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="204" mass="22047" checksum="18A53852CE8C9067" modified="2010-06-15" version="1">MPQGPRNPNEKAVHPPLSDATSDQPSQSQGARVQGRDLDLIQEKQQQRTLQNRQTTSPTIGIASPLEPGQDPQPYGGRQHRPAFGEPLSPGITVSDIHGRSRSSSITSSSSGDETQPQRPELRYSPMLIRHMTMGPEEPLRYEADIAIRGGLVRDGESMAHRRAGEEQLPVSESGVSESAATSSVTLGREVTEDVMSGEQSMDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9U3</accession>
    <name>D5G9U3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00005073001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_002837049.1"/>
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        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="General stress response phosphoprotein phosphatase Psr1/2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="TIM50"/>
    </dbReference>
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        <property type="entry name" value="HIF-SF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FCP1 homology" evidence="2">
        <location>
            <begin position="435"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="62"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="155"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="217"/>
            <end position="226"/>
        </location>
    </feature>
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        <location>
            <begin position="232"/>
            <end position="244"/>
        </location>
    </feature>
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            <end position="269"/>
        </location>
    </feature>
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            <end position="313"/>
        </location>
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        </location>
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            <begin position="336"/>
            <end position="352"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAZ81286.1"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9X0</accession>
    <name>D5G9X0_TUBMM</name>
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        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00005110001</name>
    </gene>
    <organism evidence="3 4">
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        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NPR2 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430064">
        <property type="protein sequence ID" value="CAZ81313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="eggNOG" id="KOG3789">
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    <dbReference type="HOGENOM" id="CLU_014995_3_2_1"/>
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    <dbReference type="OMA" id="IVMHKPD"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990130">
        <property type="term" value="C:GATOR1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010508">
        <property type="term" value="P:positive regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009348">
        <property type="entry name" value="NPR2-like"/>
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    <dbReference type="PANTHER" id="PTHR12991:SF10">
        <property type="entry name" value="GATOR COMPLEX PROTEIN NPRL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12991">
        <property type="entry name" value="NITROGEN PERMEASE REGULATOR 2/TUMOR SUPPRESSOR CANDIDATE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NPR2"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <location>
            <begin position="50"/>
            <end position="100"/>
        </location>
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            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
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            <begin position="86"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="CAZ81313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="450" mass="50295" checksum="3B87216F3B2E4975" modified="2010-06-15" version="1">MAWLGYVLXXXXXXXFPPPPPALHLSNSANLLKHLTNLYRAGPQVVHQVPSGSVTFTPPPPPPSTSTVSSSSSLTTAANNDGNPAPITPPLPPTSPPPASPPLFDFETVSEYIIPKQELCDRLVTVCTNKYRILGYPVCLEDRKYERNEFIFNFAIVLDEGVVEYSTYKSVIRKVAKLFKALEEQSGFLSNEVTAVGVFALIEQVLEDLNNYSECMIPINESNTINIKLFPTYAPPPQVKAFHVPVCTVQLERLMDVNWDLTMQKIVPFIDGINSVRRISELADADYNLTRKCIEHLLYYGCLIMVDVFQFSAIYACTAEISILVQDPSLQEECQAYVSYSSPRIPFPFLLQLYTSLTQGLTLKKWCMENATKLKGIDVRRFISFGIVKGFLYRSHKYPVPAEGKVDRALPLARYLDGGHHFDEICTDLQASENDVMGMLGGRGVQIVHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>D5GA16</accession>
    <name>D5GA16_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00003497001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430070">
        <property type="protein sequence ID" value="CAZ81359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837168.1">
        <property type="nucleotide sequence ID" value="XM_002837122.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GA16"/>
    <dbReference type="GeneID" id="9187064"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003497001"/>
    <dbReference type="HOGENOM" id="CLU_1662083_0_0_1"/>
    <dbReference type="InParanoid" id="D5GA16"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ81359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="159" mass="18324" checksum="6176CCB4569F8A43" modified="2010-06-15" version="1">MSNPLCLARRRWKETCFRHSAKCSPCFFRTLPIRLHNPYYSSTVQVHRIPSRLLSSLTRPQSTGRSTQTRTVVTTPAQLSKRIQDQQSTAVLKQSLKYPIQSYICWCLVAHTTTTLYSTSTVQRWTQKCKHVVILDNTEGSRAITLPCARQVGRREKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5GA17</accession>
    <name>D5GA17_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00003498001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430070">
        <property type="protein sequence ID" value="CAZ81360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837169.1">
        <property type="nucleotide sequence ID" value="XM_002837123.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GA17"/>
    <dbReference type="STRING" id="656061.D5GA17"/>
    <dbReference type="GeneID" id="9187058"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003498001"/>
    <dbReference type="eggNOG" id="KOG0158">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_14_4_1"/>
    <dbReference type="InParanoid" id="D5GA17"/>
    <dbReference type="OMA" id="DGMHETI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11062">
        <property type="entry name" value="CYP58-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050121">
        <property type="entry name" value="Cytochrome_P450_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305:SF157">
        <property type="entry name" value="N-ACETYLTRYPTOPHAN 6-HYDROXYLASE IVOC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0349">Heme</keyword>
    <keyword evidence="5 7 8" id="KW-0408">Iron</keyword>
    <keyword evidence="3 7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 8" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="7">
        <location>
            <position position="401"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ81360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="457" mass="51392" checksum="695DB033FCCD77F9" modified="2010-06-15" version="1">MAAVTGWYAGYWDLYRGGQMVNHLVDLHKEYGPIVRCEPNHLHFSSPEVYSTIYNATGKLTKDPNLYLSFGANDSVFTLVDPTIARTRREVISPMFSRRMVLSLQPLISGKFRKLCGKFSSYADRDEAADMASGFRSASIDIITQYCYGECLDSLDVEGFKHDILLSIKATSKAFWVVKYFPMDWILTLPANLTLRLMPELKGFLAVRDNIEAQVKRYMKNPSLLEKSSHPTVYQRFLDPDVKGGIPKASALADEAQNLFFAGSDTVGTTLAFGTYHILTTPGLQEKLFAEICQVWPVLEEEPTYDQLEKSAYLTAIIKESLRISHGVVTPLTRVVPASGMTIQDQPIPDGTVVSMDVPTVHLNPNIFPSPNTFLPTRWLDSNAKDLDKYLVAFSKGSRACIGSNLAWAEMYIGFAGVFRRFEMAVWETSKEDMEWMDCFTPITRGDLKVKFKVRGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAE1</accession>
    <name>D5GAE1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00005118001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CENP-I/CTF3 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430075">
        <property type="protein sequence ID" value="CAZ81398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837207.1">
        <property type="nucleotide sequence ID" value="XM_002837161.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAE1"/>
    <dbReference type="STRING" id="656061.D5GAE1"/>
    <dbReference type="GeneID" id="9187096"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005118001"/>
    <dbReference type="eggNOG" id="ENOG502QU9H">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023256_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAE1"/>
    <dbReference type="OMA" id="RVFKNYY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000939">
        <property type="term" value="C:inner kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034080">
        <property type="term" value="P:CENP-A containing chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000070">
        <property type="term" value="P:mitotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22647">
        <property type="entry name" value="CTF3_NTD_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012485">
        <property type="entry name" value="CENP-I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48208">
        <property type="entry name" value="CENTROMERE PROTEIN I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48208:SF2">
        <property type="entry name" value="CENTROMERE PROTEIN I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07778">
        <property type="entry name" value="CENP-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0137">Centromere</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ81398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="730" mass="81423" checksum="A7A6D5301140AEF1" modified="2010-06-15" version="1">MEVCCLIRSHNATCLLCPAASIRNSHSKNRIASVIDSISQHATVCGLANDSLETLIDVLATSPCYLDQSSVGSIVKILFPRRKVSENVVVKVVGSLGQGQSKPSLATQALLLRWLVMVYDFLESYDVLSQLYGVLFNLLDMITLRGHLCHILSSLTRRKHVKPFRIQALYVLLLAHKQSLGNEQPLLGLLQVYKDYYPDVIVGEITTARAGVFSHPNIEWMQKLLLIQESNADDYSGPSDTRSSFRIVRKIGGRAMKRQKTNHIAIPEVHTFRSIEASVTLEDVEDVSDFVKKLDKLELPNQLVAVLEDPLLRKLISLKPSDGANLRINNWLAACLSGELDSPSVSDLRSADRTEQFLSRVLEYTRSARRLLPAVEDFLKNYLRAWDGNSHTSTLLGLLCFLPIREFEELHRKFFTLVDCQLNGRDETSTIKLVAFYTELIRQWAITYGNQAQEIEFSEEESLALRNTLSHVGQICLVGLANFPGSNLIADAILCFYEGTCATPWENGLFRIVLPPDQVVYYFVFSGDVMTLSRICGILSKYKRGFELSLRQAQSGHPNGAGEYPRDYVNHFNGFLMDICNLVWRNRAFKWGGSDSKQDQNALGCTLANNVALSLQRAVDEREESLLTLFSLSHSAVLAQFSAECFRMLENANDSEMRGIRHAGPVTQKSLQLLATNGGLKITYSDFRVAVLRALGKKGLGGIWDFMHSTMISLIQLTSGADSSRAASLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAK8</accession>
    <name>D5GAK8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00003664001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430079">
        <property type="protein sequence ID" value="CAZ81551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837360.1">
        <property type="nucleotide sequence ID" value="XM_002837314.1"/>
    </dbReference>
    <dbReference type="GeneID" id="9187491"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003664001"/>
    <dbReference type="eggNOG" id="KOG2533">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_244611_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAK8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015225">
        <property type="term" value="F:biotin transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901604">
        <property type="term" value="F:dethiobiotin transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015295">
        <property type="term" value="F:solute:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905135">
        <property type="term" value="P:biotin import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000399">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000278">
        <property type="entry name" value="Putative MFS transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF33">
        <property type="entry name" value="VITAMIN H TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1114"/>
            <end position="1137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1157"/>
            <end position="1175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1211"/>
            <end position="1237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1249"/>
            <end position="1267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1279"/>
            <end position="1301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1363"/>
            <end position="1388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1400"/>
            <end position="1418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1430"/>
            <end position="1451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1457"/>
            <end position="1480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1492"/>
            <end position="1516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1536"/>
            <end position="1555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="233"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="405"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="490"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="745"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="949"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="246"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="406"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="426"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="493"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="521"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="567"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="597"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="757"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="953"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="973"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1015"/>
            <end position="1024"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ81551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1593" mass="172883" checksum="45944B21505F40F3" modified="2010-06-15" version="1">MDTANVSNSGGLVGAALRGGVADGLVQGQPSSSQAEFVPYQHQLVFGPGTNEYSAIPESFPHTEYNPYPSSEAWNAPSQPAHQQHAQHYTSLDPMSHFAPPPHAFPQQFTPHNFPTNQHPFAATGAVTTASPQIATAPITAARTKQSHKPPSIQQSFGPNVVRNVATSTPNAATLTTNAMTSTTNVTTSTPKANAETRVKGCPNVTVWSPHDPSLQLSLDFLKMISSVALGGEPSASVGPPKKNQKVASAASTTKAQPVKTAAPPKSKLPEDPVEKVRYQIEDALECDGDDDIEDIRKASKQVWDVIIGIRPEQSKLVEVAVRAILKVGDNQILRALGESILFSIRLRKWMTTEWNRDRNSPTLLYKFCVSEEDLEQSSWIKALKSIGSKSEDPKTREICMAITRSARERSAKREREEKAKEKQQKPASLAASSSLSDRTNDKEKSLGNGAAAKSEASSNVNVGIKRKSEDDARVGQNTIKKIALGEGKIPAVSSSPNPKTGLSAGNSKNAATPTAEKKLTTSTATSTSANGSSASAKPKTTTSGFFKSLQGNRPAIVTKPAEKKSTPNPESTPSTFTSIFDQLVRQQKEGTTGLRPDSDSKKREADEDQNGSNKKVRKKKTVRWKTGEDLARVKVIEWVEPEGEYYGGSSGHENHEYGNARNFDVEEGREALAALKNRNFLEDEEDLLDWYQPLSIDFGDLNHASTSADNAISRGGKKEIISEEAKIQSKRELQTLSALYTSPAAIPYSPREPDSNSDGSQEFGQQPTIIPLPDRLKPPTGLENSFGSVQLNPQPAAVADISSILSAINNFQQPAQSQTQATANSILAQIQAITQSQQQPAVPPSVPFTQPVAQQIAQAAPAPAQQPDLGSILMALGPNSSQQQQAGTSLLQNLVQQGSAAQGGTSAALTNILAQMSSNPTAFSFGQTIPNTYDTSNSQTWPTYEPQFHQPYGSTPNHSNQSSWATGSGTDEFGRTLREGDREKEGRGGKDVRGALREATGWDERKSEVERHNGSGNNGNNGNEPTRGGWSGVGEIEYGTSRQQPPKSLERKMTSAGVTPTSLPDKGKELSEGVQESRISGSDPELMEDREGGEDTVLDAKIERIYRKLDLRIVPAFWVLYFLCAAIRSNVGLSLTMNSKEKHSLSDRLKLTPHEISTGLALFYVCYVLFDLPANLIMTRLSPHVWMSRIVIGVGIVGSCHAALSAEWNFYLLRVLLGIVIAGMWPGMAYYLTLFYPPSRTGKRIGQYFTAAQVSAAVVGLVSAGFQKMDGYGGLTGFEWMFLLYGLVAVIDGIVILWWLPDRPRAPGTHVQPTGWRRYIPASQPALKGEDARVHYEDLTRVYHSKPWGVKDLVKILRDWRLYPLTIMYFGVVGVGIGVQNFGTLIIRATNPKLTSVELSLLFSPIWVCDLIAILLVTPISDRFHHYRALFFSVPVCIQISGLLVTTYAGGPDNPWPRYVGLLMVGFGLGPTVPICMTWTNEIFQRRHGEVGVAAASALVSGLGNLGSVTTTYALYTGWDSDNRKGPHQFRKSNLVMIGILCTSILSAVTMGLLMKFVDGKKTPEEQGAVMDRAARRELRGQRGFGRIKGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAR1</accession>
    <name>D5GAR1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Nuclear pore complex protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00003748001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Functions as a component of the nuclear pore complex (NPC).</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Part of the nuclear pore complex (NPC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
            <location evidence="7">Nuclear pore complex</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the nucleoporin Nup84/Nup107 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430085">
        <property type="protein sequence ID" value="CAZ81604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837413.1">
        <property type="nucleotide sequence ID" value="XM_002837367.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAR1"/>
    <dbReference type="FunCoup" id="D5GAR1">
        <property type="interactions" value="502"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GAR1"/>
    <dbReference type="GeneID" id="9187771"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003748001"/>
    <dbReference type="eggNOG" id="KOG1964">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012944_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAR1"/>
    <dbReference type="OMA" id="RYQGFCK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031080">
        <property type="term" value="C:nuclear pore outer ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000973">
        <property type="term" value="P:post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3450.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.190.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007252">
        <property type="entry name" value="Nup84/Nup107"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13003:SF2">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13003">
        <property type="entry name" value="NUP107-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04121">
        <property type="entry name" value="Nup84_Nup100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="5 7" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0811">Translocation</keyword>
    <keyword evidence="1 7" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="21"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="294"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="370"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="24"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="37"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="72"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="304"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ81604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="960" mass="106971" checksum="CC987F75D1F4C90F" modified="2010-06-15" version="1">MAPMMTSNSPALAFGSQKPAFSFDMQNSRGSNLFSSARPAAAAAGPRVQAQQMEGSWDIMGDDVSDGVRNDNDEDDDSRALTSEDDEQEETMPHSPGAPDGGIFDQEWPEVDAFAIALNSSNLLQSADPNSAQAATSGYDLIERFKNVSGAQLDEVKAAHMPELSHESTSDVVHWELEHQTWELFGTLSSHRLEGRIQSQDLPAELANNKYTSNQRLRQHLRETDAKFREWCLILQWLCAYAPDPSDLFDNIEELGGKGGWMYTKESLKAEKRLRGRRGKATLVFGSLGGGRSGNKITELDPDAPSRQRKQLENEDEESERFLFRAIWVFLRKGDLKGAKDLCAEMGEFWRAASLDGGEEAWDPMVDGQRFDNQSDEESMDMSENSQPEGVTGNRRRELWKRMCYAIAHRSGAEDWEKAVYGTLCGDFESVCAPGSLTILTRELWLAKMRKKTGPSCLCIVGGPSICPHEFTSTSFPAFDSTAFHSRVGSLAEDETVLSRIIDYLASLDHIKVESRVPLRVIQGSLMSLRFHELVDELYRQLLAFRDIPDYDPLHEEDSIGVDVTDCRTLRIVVHILLVLQLLNAGFTEDTPFHVQAEALIAAYIEFLAEAGKVKLIPLYAGQLSPQKAIDVMGTILNKIREEPLRLELLELMRAHNIDYVGCLKKGMEIALDLTKNHYESERRHVATLSVTVGEILEEDNLLIQGLEWLVLGGDELKAESLKCATVIYKRLLLSGRLAAAKRVSQTIRSTDLIPEGEDVNHFAAGGIMEDDEFRGVFDSDVDQWALAVVIEMEVFIQALDELEAWNELLGQKTNRHHLDRARKAALSEAYSSTNHKLGKVIRSWLHSTLSTVPPFPPQSHIIKPSSIVAIRNIYIPELTFALHDVYIEAGKHVSKNLLGNTLELATVVADPNGSVLKCFTETARVEEYLRALGLASRSVLGASSEKNNNNKGLGIWKVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAZ2</accession>
    <name>D5GAZ2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Clathrin heavy chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00005369001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Clathrin is the major protein of the polyhedral coat of coated pits and vesicles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasmic vesicle membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
            <orientation evidence="6">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <location evidence="6">Coated pit</location>
            <topology evidence="6">Peripheral membrane protein</topology>
            <orientation evidence="6">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the clathrin heavy chain family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430086">
        <property type="protein sequence ID" value="CAZ81685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837494.1">
        <property type="nucleotide sequence ID" value="XM_002837448.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5GAZ2">
        <property type="interactions" value="1175"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GAZ2"/>
    <dbReference type="GeneID" id="9187853"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005369001"/>
    <dbReference type="eggNOG" id="KOG0985">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002136_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAZ2"/>
    <dbReference type="OMA" id="HCYDLLH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030132">
        <property type="term" value="C:clathrin coat of coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030130">
        <property type="term" value="C:clathrin coat of trans-Golgi network vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071439">
        <property type="term" value="C:clathrin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032051">
        <property type="term" value="F:clathrin light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006898">
        <property type="term" value="P:receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000001">
        <property type="entry name" value="Clathrin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000002">
        <property type="entry name" value="Clathrin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000005">
        <property type="entry name" value="Clathrin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000082">
        <property type="entry name" value="Clathrin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.730:FF:000003">
        <property type="entry name" value="Clathrin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.110:FF:000003">
        <property type="entry name" value="Clathrin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.110">
        <property type="entry name" value="Clathrin heavy-chain terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055358">
        <property type="entry name" value="CHCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000547">
        <property type="entry name" value="Clathrin_H-chain/VPS_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016025">
        <property type="entry name" value="Clathrin_H-chain_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022365">
        <property type="entry name" value="Clathrin_H-chain_propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016341">
        <property type="entry name" value="Clathrin_heavy_chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10292:SF1">
        <property type="entry name" value="CLATHRIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10292">
        <property type="entry name" value="CLATHRIN HEAVY CHAIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00637">
        <property type="entry name" value="Clathrin"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13838">
        <property type="entry name" value="Clathrin_H_link"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01394">
        <property type="entry name" value="Clathrin_propel"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002290">
        <property type="entry name" value="Clathrin_H_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00299">
        <property type="entry name" value="CLH"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50989">
        <property type="entry name" value="Clathrin heavy-chain terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50236">
        <property type="entry name" value="CHCR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0168">Coated pit</keyword>
    <keyword evidence="5 6" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="3 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="467"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="617"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="765"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="911"/>
            <end position="1056"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="1060"/>
            <end position="1201"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="1206"/>
            <end position="1352"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="1355"/>
            <end position="1498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR002290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ81685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1602" mass="181864" checksum="86F9722B172F0D7A" modified="2010-06-15" version="1">MHWSKQIIALKAQRTLQIFNLEAKEKLKSHTMNEDVVFWKWISERSLGLVTDNSVYHWDVMDPSQAAPVKIFDRHMNLSGCQIINYRVNADEKWMVVVGISQSQGRVVGNMQLYSKDRGISQAIEGHAAAFASIRLEGASSDTKLFTFAVRTGQGAKLHVVEVDHVAANPVFQKKAVDVYFPAEAVNDFPVAMQISPRFSIIYLVTKYGFIHLYDLETGACIFMNRISSETIFITAPHEASSGIIGVNRKGQVLCVSVDENQIIPYLRENPANSTLAVKLASRAGLAGADELYQKQFQLLMDQKNFVEAAKVAANSPRGFLRTPATIEQFKSVPAPPGQLSVILQYFGMLLDKTTLNKHETIELVKPVLAQNRKHLLEKWLKENKLECTEELGDAVRVHDVNLALSVYLRANVPQKVVASFAETGQFDKIVPYAQKVGYSPDYTALLTHIVRINPEKGAEFATQLASQEGGSLVDIDRVVDVFLSQNMIQQATAFLLTALEGNKPEHAAQQTRLLEMNLLNAPQVADAILLNNMFSYYDREKIAKLCENAGLFQRALEHFDDPAAVKRVLCNNSAGLSPEFIVEYFGRMSLEQSLDCMREMLRVNIRQNLQVVIQIATKYSDLLGANRLIDLFESFKTFEGLYYYLGSIVNLSEDPDVHFKYIQAATVCGQMSEVERICRDSNFYNPEKVKNFLKEAKLTEQLPLIVVCDRFNFIHELVLYLYQNQQFKSIEVYVQRVNPARTPAVVGGLLDVDCEESIIKNLLKSVQSSAIPIDELVAEVERRNRLKLLLPFLEDTLAAGNQQPAVYNALAKIYIDSNNNPEKFLKENNQYDNLVVGKYCEKRDPYLAFIAYQKGQNDVELVRITNENSMFKQQARYLVARRDSELWGFVLSPNNIHRRSLVDQVVATAVPESTDPEDVSVAVQCFLSNDLPTELIELLEKIILEPSTFSDNSSLQNLLILTAVKADKARVMDYIHRLTNYDGPDVAHIAIQNGLYEEAFEVYKKINAHSDAINVLIENIVSIERAFGYADRVDLPEVWSRLGKAQLDGLRITDSIESYIRAKDPGNYAEVIEIATHAAKFEDLIKYLQMCRQTLREPAVDSSLVLCYARVDKLPELEEFLNSSNVANIEECGDKAYEEGLHQAAKVFFSSISNWAKLATTLVFLEEYQNAVECARKANSTKVWKQVNEACVSKKEFRLAQICGLNLIVHAEELQDLVKQYEHNGYFDELISLLEAGLGLERAHMGMFTELGIALSKYHPDRVMEHLKLFWGRINIPKMIRACEQAHLWPELIFLYCHYDEWDNAALAMMERAADSWEHQSFKDIVVKVANLEIYYRALNFYLQEQPSLLTDLLTVLTPRIDVSRVVRMFEKSDNIPLIKPFLLNVQPQNNKIVNNAINDLLIEEEDYKTLRDSVENYDNYDPIELAQRLEQHELVFFRQIAANIYRKQKRWNQSIALSKQDRLFKDAIETAAVSGKEDVAEELLRYFVDIGSRECYVGMLYACYDLIQLDVVMEISWRHGLSDYTMPFMINAMRQQTKKIATLEKDNEERKIKEATQQKVEDTTPILGNRLMLTAGPGGPMPQQSYANGIAPQATGFGGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>D5GB97</accession>
    <name>D5GB97_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00000531001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SAT4 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430089">
        <property type="protein sequence ID" value="CAZ81790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837599.1">
        <property type="nucleotide sequence ID" value="XM_002837553.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GB97"/>
    <dbReference type="STRING" id="656061.D5GB97"/>
    <dbReference type="GeneID" id="9181501"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000531001"/>
    <dbReference type="eggNOG" id="ENOG502T73W">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_824369_0_0_1"/>
    <dbReference type="InParanoid" id="D5GB97"/>
    <dbReference type="OMA" id="SCITVAW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049326">
        <property type="entry name" value="Rhodopsin_dom_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052337">
        <property type="entry name" value="SAT4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048:SF47">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33048">
        <property type="entry name" value="PTH11-LIKE INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_5G11245)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20684">
        <property type="entry name" value="Fung_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="65"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="108"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="147"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodopsin" evidence="8">
        <location>
            <begin position="6"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="242"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ81790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="337" mass="37975" checksum="1E8BC1922ADF61BC" modified="2010-06-15" version="1">MACYADLNAGIGRPLWAVSAKQFELWFKCTLASSFLYPVMMSTVRISVLLFYRRLIGPTYQTLQYCIKALILLTIPYVVTYEFLLGFMCKPPSSFWKPFQRNADCGDVYYYKLHITLYSVSLVFDILILVLPLKAIWELKMQKRQRVLVCALIALGASACFGIAYRLALWVLEMKRYPYIDPRWTANPLSKVIPPQFDRYGWTYWVPSLLESNMAIIGASLPALKPLLTQYNCLQRSKFSKFSQGGSGGMTDPSDTKRSHGIQRPGPYSEQIGSNNDSMELLHVDAVGGTFSGTYPVKHSHVTTIKADVRDDLSTDSVLPGSIVQTSTVEVRVSEKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBA1</accession>
    <name>D5GBA1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00000535001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430089">
        <property type="protein sequence ID" value="CAZ81794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837603.1">
        <property type="nucleotide sequence ID" value="XM_002837557.1"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GBA1"/>
    <dbReference type="GeneID" id="9181505"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000535001"/>
    <dbReference type="eggNOG" id="ENOG502SRE6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_251910_0_0_1"/>
    <dbReference type="InParanoid" id="D5GBA1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024983">
        <property type="entry name" value="CHAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12770">
        <property type="entry name" value="CHAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CHAT" evidence="1">
        <location>
            <begin position="219"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1440" mass="162409" checksum="3EA8F078D9DEA94B" modified="2010-06-15" version="1">MSQLRLLEDAFREFLNIEEKQEIPAVIQIREISTTLNIVGEATWKASIQCDEGRFVRMVEVKNPFNANDEENLEWYLEEFATTSPLSRSRAHEVSSNLKEYAAGLIKGFALEEVVNHIRAQNAVGEEPGITFFLDVETLPSESAKSSLHIIHWEVLENLDLWPREIKGRIVVRRKVADLELKQSSPVDLVNGAFNILLVVARNLSIGEDEIGHTLVAAELVKVLVDLPIETPVDLEIVRPGTWETFKAKLEARPRGYYRLVHFDLHGRVRVDKLAGEKASSLCFVSDKDPTGVTWKSASDVADLLYLHNVTSVILNARNSADASEESVALKFINRGVSAVVAVSYEFMASAAGSFMTGFYRSLLIDRSSFAVAAHAGRAKMMKQKMRDSSYGIRVPVEDHMVPVFYQRGELAAINWDVMRKLNMILLTNGLKAPMNLVGRELDILRLETLLLCGDGIAAVTGRRRVGKTALLKHLCWWWNITGLVNHSQYIDLEATQDTFCEILLLLYRDAIGMPTILEMEMIVVPDARETDAYWRLVNWLQLNHHLIVFDGVEHWKPEIPEKVQPADRTGPTFWNAQRRALESFLEDVSGGETIVIVTAELNSPWVDINPYALEGLSPYYMASLLPIELEKSNEETVLGPGANFMEFYAQLIELCDGLPGAVTEFSCKLPLNIKENTPEEVYCRSLLAVPAAAGRYSSDCARAEFVQSLIPPNSDEAPHSDLNRLIYIIAPFWGRIPKNIDDYLIFICFHGYFNRDDPKTLCLPIRRFDAKNPRDLSPEEIKRVMELLGRSKIRSEYHRITSSLQEKGYLTKDKFKPDQYYRVNPVLTLALRGVIFGDGHLSTVAHARACLAAFLDYYKIQTLKDNWGSRIQLEKTSIYSIMITLSCLPPTLSGLSLLPVRPMLSLISTYQAGRGGSDWHILDAISEMLINLFDSFLATERDIPLEAVFTAYKLVFHCSNYLCSRYRNADGATDKFIEQVKKSNEYSKLSEALPESLRENLLIANFMAATMSAQVADAHRELLPGAFVYETMGDVSGANREAHKLLESDPRIDFQAEGNSDAAQDFRRLQHLRTLSLEFDMKNVRGWSSKTEKAKAQKSIKEVCNELRGSAAKLGLDLTYIDGWLAVHEVRVGVITLTETLSKLAKAEANDPKYLPGSAIMEAEIKRDSGDIASAKEILLTALEHCREGGSAQGELLCHGRLQEIAYLTGQWGDTITHNERMIELVDIVYSRDTKEKRRADKAVLAAHSALAHIHLRQWDEAIECVLYTIRICQEAPVDDPNAFLLDRVQAEQLVILRYLRYHHPDHEDLPSSAEIRRQQLALQRKLDPAMAKKMDDPAVRRMEEIKTQLSLTVAMSPRAQWAAGREIWDSDVPMYDDGIPLHTRNWGVTEWWEDKEEDAEALALVKEYHDSFQYDPESALFGISLDESKALEGLLPGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBJ2</accession>
    <name>D5GBJ2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Delta(14)-sterol reductase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">C-14 sterol reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Sterol C14-reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">GSTUM_00005658001</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the ERG4/ERG24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ81998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837807.1">
        <property type="nucleotide sequence ID" value="XM_002837761.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBJ2"/>
    <dbReference type="FunCoup" id="D5GBJ2">
        <property type="interactions" value="457"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GBJ2"/>
    <dbReference type="GeneID" id="9185433"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005658001"/>
    <dbReference type="eggNOG" id="KOG1435">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015631_0_3_1"/>
    <dbReference type="InParanoid" id="D5GBJ2"/>
    <dbReference type="OMA" id="EWCELRP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050613">
        <property type="term" value="F:Delta14-sterol reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1630:FF:000008">
        <property type="entry name" value="C-14 sterol reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001171">
        <property type="entry name" value="ERG24_DHCR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018083">
        <property type="entry name" value="Sterol_reductase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21257">
        <property type="entry name" value="DELTA(14)-STEROL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21257:SF52">
        <property type="entry name" value="DELTA(14)-STEROL REDUCTASE TM7SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01222">
        <property type="entry name" value="ERG4_ERG24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01017">
        <property type="entry name" value="STEROL_REDUCT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01018">
        <property type="entry name" value="STEROL_REDUCT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 14" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10 14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="8 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="13 14" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="9 14" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="12 14" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="71"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="101"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="142"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="261"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="291"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="373"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU369120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAZ81998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="424" mass="48413" checksum="E8BEAAEE03D6D734" modified="2010-06-15" version="1">MPPPAHGYEFGGPLGAFAISLAAPAATYALYFVCNEDGCPSSSFLRDPVGNIASKWPGLSGAFDWGVTKHYFLWYFALAIMYFVLPGKIGDGVVLSNGRRLKYKFSGSASLLTVFAYCGFMTYKEGFQWPFWAYIWDNQLQLMTAALISSIILAFYCYISSFFTGELLANDTGNFIYDWFLGRPLNPRIGSFDIKVFSELRPGMILWPILNFAFMAHQYNTHGKITDSMILVNAFHLWYVCDALHNEPAVLTTMDVTTDGFGFMLSFGDLMWLPFNYSLQSRYLAMHPFELGLLGVVGVLAVQGLGYWIFRRANREKHDFRTDPNDPKLAHIKYIETKSGSRLMITGWWGIARHINYLGDWIMAWAWCLPTGYRGPLSYFYVVYFAILLLHRDRRDEAKCKLKYGADWKRYTDLVPSRIVPGIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBK0</accession>
    <name>D5GBK0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00005671001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ82006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837815.1">
        <property type="nucleotide sequence ID" value="XM_002837769.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBK0"/>
    <dbReference type="STRING" id="656061.D5GBK0"/>
    <dbReference type="GeneID" id="9185441"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005671001"/>
    <dbReference type="eggNOG" id="ENOG502QW7S">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037235_0_0_1"/>
    <dbReference type="InParanoid" id="D5GBK0"/>
    <dbReference type="OMA" id="EDWATMG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000432">
        <property type="term" value="P:positive regulation of transcription from RNA polymerase II promoter by glucose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018818">
        <property type="entry name" value="Stb3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28164">
        <property type="entry name" value="PROTEIN STB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28164:SF1">
        <property type="entry name" value="PROTEIN STB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10330">
        <property type="entry name" value="Stb3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="189"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="229"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="233"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="265"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="336" mass="35277" checksum="AA888AC8509D7B69" modified="2010-06-15" version="1">MATITTTTATSMSVAIPTSRPHRGGKATVSDNNAFTLPEPYSHFPTPPNSISPSLPPVPNPVTASADSDIDVPDIITTTSTTPVHHQLKAAPGTSLLAETTGAITSSLLAKYHLPEILLKHGPLAIRHLTAHLISAIPGFSSIPPAKQRRLVVGALEGRGGVSGSEGEAGGVNGDVVFDKVGWGRWDARVKSDPPRSHRGISPATSDHSSFVKTKAGIVGRSYTGESGIFVQSEEEGDESMDSEGVEGEEEEEDGVDRMSLDESSSSEDEEDEDDGDVTDEEDWAQMGAAMLRNQVGSPISPTGGGFDNLSTAWGASVRAREEREAVEALVKLSSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBQ3</accession>
    <name>D5GBQ3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00005521001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ81903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837712.1">
        <property type="nucleotide sequence ID" value="XM_002837666.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBQ3"/>
    <dbReference type="STRING" id="656061.D5GBQ3"/>
    <dbReference type="GeneID" id="9182940"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005521001"/>
    <dbReference type="eggNOG" id="KOG2582">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028825_1_1_1"/>
    <dbReference type="InParanoid" id="D5GBQ3"/>
    <dbReference type="OMA" id="NHYHDLV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008180">
        <property type="term" value="C:COP9 signalosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055089">
        <property type="entry name" value="COP9_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050756">
        <property type="entry name" value="CSN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10758">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 3/COP9 SIGNALOSOME COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10758:SF1">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22788">
        <property type="entry name" value="COP9_hel_rpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="COP9 signalosome complex subunit 3 N-terminal helical repeats" evidence="2">
        <location>
            <begin position="46"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ81903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="479" mass="53900" checksum="7F25C2C0F0CBE967" modified="2010-06-15" version="1">MDKLIDSFLSFPSSPNISESELQKQIQNHLSLLNRAPAATLAYQHAGTDVLALLDPSVNSLAYLYVLVARIDLGQKDAMEVVWRQVVTFLETFNPKQVRCAQDIFRRLVEYFFSFATESGKPIIAVRPIKKAILRFNPAKFSFLHPLFLRLCLEAKCYRDAAAIVDIDIVEFPCASTGKVVENARAGEVTYQDVLTYFLYAGMIYMGIKEWRRAADYLTYAIVAPGSACSQIQIDAYKKYILVGLLLDGKSLPIPRSTTSVANRAYKALSKPYESFVVAFKAGNPELLRTEVTQLVDVFRQDNNTGLAIQCLEAFRRMQIIALRDTYVTLSTEEISKKDLEVTGRGGDMGGKEGTEAVILGMIEREEISASLSHSHPNPEQSQTTVHFHNHPVDESCNLAALQVELRRIMTLNKQMKTINKKYGMSREFIQYMVKMAKMSGSSVLAGMAEPDLMDFDYGFSWAEDEPEETIMQDFEDDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCE8</accession>
    <name>D5GCE8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00005856001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430109">
        <property type="protein sequence ID" value="CAZ82191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838000.1">
        <property type="nucleotide sequence ID" value="XM_002837954.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCE8"/>
    <dbReference type="GeneID" id="9186373"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005856001"/>
    <dbReference type="HOGENOM" id="CLU_360637_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCE8"/>
    <dbReference type="OMA" id="YVQEMPP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="172"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="182"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="440"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="462"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="504"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="516"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="549"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="585"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="604"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="650"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="723"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="744"/>
            <end position="761"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="776" mass="87374" checksum="79D1CE9650AD2DDB" modified="2010-06-15" version="1">MPTDVLKPGPGNSDPLQESEEDLREYVKIIRLRDKIRDGMHPRFKPANHPRSSQATTPQPLQKNPSTSSDGAQLSQAPGLQAPQCTDFKINNVFLTKSDTLVKAELRLKREKIEKELQKRKLEVEAKVWTGAEPVEHINLWDPSTDVFSDQLESIMKKAGVSFKETIRSINSAPSKPVAEGNENSETAIRSGNPETHQDRHMTRKTWSGPVESTLPTVVEASRRPPKESLTPRQFSSDKETPTVEESTSQRLAIDTAYEPPKAQAPAVPSPKGANAPLDVNNDGYEPAPAHGRPYSPPASPRYSTTSHLKSPAAPQPVRPANMARVESQGPRGIVEIEDDGDEIPYSRYAGNPERELRRDNVSPISPVPLIKQEPRSPFPDVHPPRVTRVGGPADTYDERQRHDYPPIPPPPQRYSYQPTVHPHPPAAGHHVYDSFGYHLPPPPPPPAPAPVYTRYPPYDPEYYHAPRPYDEYDRRYAAPSAVPSRLPTRSGRRSASPVNYSQRSPRSVSPSRVRNPKDSRPVSRAEYRPPSRMTRPPSPHRLDMYSRPYPPPQPPMYRPEYPGDYASYYGPPRERPTGYYDPYRSMRFDEYPPRPESSVIPRGFDDRSYRTRDYPYVPARPSMPPPQPVTGGYAPEHRADYPSSSRASVRPEDHLERRVEYRPEASRASPRREDRPYSNGVDNYFHGREPSVRHGSVRPDPTRAYGEGEYAPRRQASVRPDAGFRDRFADPGPREPGHMQPPHLVPPYAHPAPPHPPLPPFAGSGYSEGRYRGNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCQ1</accession>
    <name>D5GCQ1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00005963001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430120">
        <property type="protein sequence ID" value="CAZ82294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838103.1">
        <property type="nucleotide sequence ID" value="XM_002838057.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCQ1"/>
    <dbReference type="GeneID" id="9187913"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005963001"/>
    <dbReference type="HOGENOM" id="CLU_2016889_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCQ1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ82294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="123" mass="13079" checksum="A3E6C2FB862CED79" modified="2010-06-15" version="1">MSASSVSSLELHRCSYHPHLILAVHPFRFYAENSAASSSSVASARCSLLAILRTTSSLIPLFSFLSPKIFLVYTPTRINGVSGGLGGVWILCLGLSDADNMLSNMYDMIPRGSAGGIKVSTLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>D5GD80</accession>
    <name>D5GD80_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00006084001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CLPTM1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430142">
        <property type="protein sequence ID" value="CAZ82473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838282.1">
        <property type="nucleotide sequence ID" value="XM_002838236.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GD80"/>
    <dbReference type="STRING" id="656061.D5GD80"/>
    <dbReference type="GeneID" id="9183937"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006084001"/>
    <dbReference type="eggNOG" id="KOG2489">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019907_2_0_1"/>
    <dbReference type="InParanoid" id="D5GD80"/>
    <dbReference type="OMA" id="TLWAHFY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008429">
        <property type="entry name" value="CLPTM1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21347">
        <property type="entry name" value="CLEFT LIP AND PALATE ASSOCIATED TRANSMEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21347:SF0">
        <property type="entry name" value="LIPID SCRAMBLASE CLPTM1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05602">
        <property type="entry name" value="CLPTM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="341"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="378"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="404"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="467"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="492"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="589"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="597"/>
            <end position="609"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ82473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="633" mass="71339" checksum="CF05282657B838EC" modified="2010-06-15" version="1">MPAPNSQIAGAGEEGSQSVIKSVINGVLMFVAIQTAMKFIIPKAGTPATTTPSSFEERTSPDDPSTYMFVPEKVFPLWPAEEPVDISIYVSELLALPPLSSEQMASSLVFNEKNFQADDWSEHRSVTVDIPLSQHVQNNGTLFAHIYVSRSGSAMDPTDPDYDSSKAFRIISLLTKYMPKKKIVKTKKLIGGDDQESGDEEVAVPDNSGSIVSYFHPNLTLDVVNNADIISFNTLPPPLRQHVVLEATGARDSTGKNGWYYPIVFVNEFWQLKDHMIELNSTVKSIPMHINLAPTSWWKFQMYSSFDASFKQTVSNPGIGGSANTGAELEELKRVLVETNIFLLGTTFVVSILHTIFEMLAFKNDISHWRNKKDNVGVSVRTILANVFMQAVIFLYLLDNSDGTSWMILAGQGFGILLEAWKITRMVNVKIRPVNSFIPYRVIFEDKHKLSNLEKETKEYDEEAFKYLYWVAVPLLLAYAVYSVLYDTHKSWYSFIITTLVGSVYAYGFLMMVPSLYINYRLKSVAHMPRKTMIYKLLNTFIDDLFAFTIKMPTLHRLATLRDDVIYFIALYQAWIYRVDYRRVNEFGQGGADDGEDDRKEIEAKKEFEGATPVGVGAKTNGADKGKKATKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDD8</accession>
    <name>D5GDD8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00006165001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430142">
        <property type="protein sequence ID" value="CAZ82531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838340.1">
        <property type="nucleotide sequence ID" value="XM_002838294.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDD8"/>
    <dbReference type="FunCoup" id="D5GDD8">
        <property type="interactions" value="481"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GDD8"/>
    <dbReference type="GeneID" id="9183994"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006165001"/>
    <dbReference type="eggNOG" id="KOG2704">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011340_5_0_1"/>
    <dbReference type="InParanoid" id="D5GDD8"/>
    <dbReference type="OMA" id="WHGTRPG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003841">
        <property type="term" value="F:1-acylglycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047184">
        <property type="term" value="F:1-acylglycerophosphocholine O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046474">
        <property type="term" value="P:glycerophospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030258">
        <property type="term" value="P:lipid modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049941">
        <property type="entry name" value="LPLAT_7/PORCN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13906:SF4">
        <property type="entry name" value="LYSOPHOSPHOLIPID ACYLTRANSFERASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13906">
        <property type="entry name" value="PORCUPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="225"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="377"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="416"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="445"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ82531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="542" mass="61657" checksum="28EF50E375C0EC12" modified="2010-06-15" version="1">MISFIDYPFIWLEGATGAAKDELKLIFTILLSYPFAGVLKRLPDAEPAKKNLFMIGVSLFYMVGVFNLWGGLRTLSVSAGGAYAIAKFVQGPYMPWIAFVYLMAHMLISHIERELHGIPGVVDVTGAQMVMVMKLTAFCWSVHDGRLPEKDLSAFQKDRALPVMPSILDYIAYVLFFPSLFAGPAFDFAEYQRWIDCTMFDVVSPGATKGSTKRKRKIPKSHRPAIWKAICGLGWIAAFMKFSSWYKTGFVLDDGFLRYSVLRRVWYIYALGFTERLKYYGVWSLTEGACILSGLGYNGLDENKRPRWDRLTNVDPWALETAENTRAYLESWNMNTNKWLKNYVYLRVTPRGKKPGFRSSLVTFGTSAIWHGISPGYYLTFLTASFVQTVAKYFRRHIRPFFVEPDGKTPGRYKRAYDIFGLLATQIGFSYIVAPFIILDFKNSLIVWGRLKFFVHIGILLSMLFFNSSGKSWLSQKLKARVGRAASSEGLHKSPSASDFRGGALGLLDPMKEIEDMQRELLAKEEEVMNRRNSMNTTSKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDU6</accession>
    <name>D5GDU6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="13">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00006279001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the ZNF593/BUD20 C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430153">
        <property type="protein sequence ID" value="CAZ82689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838498.1">
        <property type="nucleotide sequence ID" value="XM_002838452.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDU6"/>
    <dbReference type="FunCoup" id="D5GDU6">
        <property type="interactions" value="365"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GDU6"/>
    <dbReference type="GeneID" id="9184484"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006279001"/>
    <dbReference type="eggNOG" id="KOG3408">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117291_2_1_1"/>
    <dbReference type="InParanoid" id="D5GDU6"/>
    <dbReference type="OMA" id="MKDHFRS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043021">
        <property type="term" value="F:ribonucleoprotein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000299">
        <property type="entry name" value="Zinc finger protein 593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051879">
        <property type="entry name" value="C2H2-ZF_Maturation_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022755">
        <property type="entry name" value="Znf_C2H2_jaz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46095">
        <property type="entry name" value="ZINC FINGER PROTEIN 593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46095:SF1">
        <property type="entry name" value="ZINC FINGER PROTEIN 593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12171">
        <property type="entry name" value="zf-C2H2_jaz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="47"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="103"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ82689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="122" mass="14235" checksum="28CA10C138FB4514" modified="2010-06-15" version="1">MGAIRRSKTKRRTRDMDQVHSDLRDPKQLAQLKALVPDEDKPGLGQFHCVECAKYFESEWNMVQHRRGKNHKRRVRLLKEEPYSQKEADAAAGIGTAAFYQASEDRREQEKMDQQFGSMEPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDV3</accession>
    <name>D5GDV3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00006288001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430153">
        <property type="protein sequence ID" value="CAZ82696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838505.1">
        <property type="nucleotide sequence ID" value="XM_002838459.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDV3"/>
    <dbReference type="STRING" id="656061.D5GDV3"/>
    <dbReference type="GeneID" id="9184491"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006288001"/>
    <dbReference type="eggNOG" id="ENOG502S71G">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108574_0_0_1"/>
    <dbReference type="InParanoid" id="D5GDV3"/>
    <dbReference type="OMA" id="WMMVMAS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="187" mass="21962" checksum="E65E7905200A83BB" modified="2010-06-15" version="1">MSDVYMERELVPVKVRRYRWPELLLAVLLLILFMCGAIVLGSFAYFTAIQRRLFIMVPWYYYFLLAISTLTILLVFSVVALYYVHLLLPIIVMISSFIMFVLWLTGLIKASIELWGPLGSINDNCVRYVYNDQFWGGRNLDTLARIQQEGVCNLWKTTFALEMISTFVFLWLILMSWQVISRARRGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>D5GE06</accession>
    <name>D5GE06_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00001133001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430158">
        <property type="protein sequence ID" value="CAZ82749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838558.1">
        <property type="nucleotide sequence ID" value="XM_002838512.1"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GE06"/>
    <dbReference type="GeneID" id="9183085"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001133001"/>
    <dbReference type="eggNOG" id="KOG0988">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_240913_0_0_1"/>
    <dbReference type="InParanoid" id="D5GE06"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031380">
        <property type="term" value="C:nuclear RNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003968">
        <property type="term" value="F:RNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030422">
        <property type="term" value="P:siRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007855">
        <property type="entry name" value="RDRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057596">
        <property type="entry name" value="RDRP_core"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23079">
        <property type="entry name" value="RNA-DEPENDENT RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23079:SF14">
        <property type="entry name" value="RNA-DEPENDENT RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05183">
        <property type="entry name" value="RdRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RDRP core" evidence="2">
        <location>
            <begin position="851"/>
            <end position="1495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="210"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="496"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="651"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="292"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="312"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="382"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="496"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="515"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="548"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="608"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="694"/>
            <end position="705"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ82749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1700" mass="192397" checksum="DE8BAB4427A51890" modified="2010-06-15" version="1">MTSPKAQLAPATTSRGPLATVRMNPREESNDTELGRRLHKLNRVYGFHTHPKQPRPNWSGTRSSGPVTSPWVQKLDALLEFSLKEVDDSFHLLGQVVKKRGIPKDRKPGVDVRVELEKAYESILDEKLAEFRDRDESVVGLFGIGSGDCPKEEPKTWPCNERYEAQKVTQDFATLNLGSNRNREDIVFKGRGKNRCEEVISMESLAASRERKNTNMVQDMPREGSSQRRRQRKILSPMEMITGGEQRSPERPRNRKSQYELAKPKKMQRAGLAQHRYEMEVAGNEQEAPRWGWEKREEEEGEGYKHRPRQTIRKEERERLRQEEQERRFGGTRALLPRRLVGEDLGDPRQGVQEPSWRKRDRQPEPPFHTVQRPAQLVAGYEDNDTESDSDDTFHSASSSVAEPKWERKAVSTELEDEYGVSSIRVSSSKDHERRPILLGQRRRKEIRVPVKPVHGSGYSHASGSYGDPFHHSLSAASSSAPSSFVSSVFTRSITAGSFSSPKNSTFQTDITGDDSFRDGKEKPILYRSDTDQYDPQGHRTDNATEDDRTDVDEPEEDECFYPQEMIIPRPQARTSRRKQKSAALRQAESFKHTPLSPVPQSVVASETGEREERERAELVGFFDEKPLGPELLRAAEETGNVHWDFAAQHEAEQSPDGVSDKGGSWPEASRQVIPGTPQRPSPGPSEQSYEGGKVCGEGPGGGGAIDDEDPEFRVDAAFEAEMLRREEALNQGSANEPLRDIEHPLHTLPFHVQYEAARVALSNGVFPTNSDVSDSIHNYCDDLFAAKTYAEVLGIMKQYCPGAAMLEKSAESAWRGYKGRTGWTDKLYMTGRVSLKDPKARKLEDIAFDIQLNPLGRSTGNRFFNRFGSDRFLTLRLPDLTSRGQNGSTPGGMVAQRVEDWLVNEEIELANRKWRCFYAKEGKSRKRSLEDEKAFINETFVQAVLFATSGVGIGDDRDEMARLGFRGLDRKIRREMSVEDLLKWHIPLEGNEDVTVPKFWSRISLGFSTAVPSFKFEPSQVEIVRDMKSTTGEVMNDGCSVVSPKVMQEIRRILDLHETPTAVQARLGGAKGVWMIDPSIDWSSDEIFIKVTDSQLKYSGYADDQDWARMTLDILHISHEPRPATINIQLIPILENRGVPFQALKELTEEHLEEDLDELFQVIDKPVELRKWIYNRGSVGRARISFNGIQVTGSMPSTKHEMAILLLESGFLVRSCRVLMDHVRVMIDQYCEELKEKLHIRIPHSTTLLCVADPTGTLREGEVSLRFSNGFLDPKTLRRTQVITGDILVARNPAHIPSDIQKVKAVDIQPYHSLALRELQDVIVFSTQGDQSLASLLSGGDYDGDKPWVCWEERLVKPFTNSPTTYVEVDAMVAPWFNKSQTRVSGLNPSQPGFFERFLQIGLRSSFNDNFLGQCTSIWEKRCYEKNDIADLEALKLAKLCSLLVDAPKQGMSLRPGKVDEIRRKFSGAPIPAYKEKGSRVRAGATHIVDRLFQVADAKVNEKQQAFHDDIGKSEGQRDGDIVELFVREHEFAQKDETSPLWITLRHLTKELTLLRQDWADWIRNKRGGPDEFRAGVPNFLQRYRDIMPPAEQARDPVVARWIREGGASFTPWAILRASAAYSGWGGERGLVWYMAGIEICWIKCQKVSMRDSELGPRTMLDSMYAPLVTSTKFMSAMRDKVDGDVEVVDMEDEVDGWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GE14</accession>
    <name>D5GE14_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00001176001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat MET30/SCONB/SCON-2 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430161">
        <property type="protein sequence ID" value="CAZ82757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838566.1">
        <property type="nucleotide sequence ID" value="XM_002838520.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GE14"/>
    <dbReference type="STRING" id="656061.D5GE14"/>
    <dbReference type="GeneID" id="9183095"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001176001"/>
    <dbReference type="eggNOG" id="ENOG502S0AR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030037_0_0_1"/>
    <dbReference type="InParanoid" id="D5GE14"/>
    <dbReference type="OMA" id="DRRFFPR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039719">
        <property type="entry name" value="FBXO28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13252">
        <property type="entry name" value="F-BOX ONLY PROTEIN 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13252:SF9">
        <property type="entry name" value="F-BOX ONLY PROTEIN 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="F-box" evidence="3">
        <location>
            <begin position="10"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="2">
        <location>
            <begin position="125"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ82757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="481" mass="52360" checksum="0EBBA54CB97ADE21" modified="2010-06-15" version="1">MTIDMRPKKALFLLCLPDELLVEIISHLPPKDVIPLHATSRRLRALTRLDPTLWRSLCSYREGSSLTVLTPPRTPISDVECATIDWYTEYKFRNAPTRMLWVGTGSPTVGDEVKGIGAFPSRGGSTEDDMVVAATSDGSVKIWSLQDNCAAREIGKSERGLIFSQKKRTPALEAQQKIREAGIVDGVSIDAAAGRIWVAGEDGLVEIDLTSLKRISTHRFPFSIMALSQTSSPYPLTIGTTLTLHMHDSRVSPNSPPPAHAPLFQPGPLSILHSGSSVKVAGRFPSLLSYDRRGWPKLTSTIHSGARLCGLTTIGTSGLAACGEYNGRGTLEIYPGLGSPYPIVKNRQTAARSKILTVTSHGSYLVTGNAEGCISFFERDARTVIREHWVEGGGKTSSGIWNVGEDGGDVIRRVKPLRGGDGKILVWAGDRLGLLGVGKERQEIPEEAVRPESEEEEYLTKMRELLRRQGEEVGILEGMGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GE54</accession>
    <name>D5GE54_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00006384001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430164">
        <property type="protein sequence ID" value="CAZ82797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838606.1">
        <property type="nucleotide sequence ID" value="XM_002838560.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GE54"/>
    <dbReference type="STRING" id="656061.D5GE54"/>
    <dbReference type="GeneID" id="9183129"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006384001"/>
    <dbReference type="eggNOG" id="KOG2179">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003639_0_1_1"/>
    <dbReference type="InParanoid" id="D5GE54"/>
    <dbReference type="OMA" id="TWPGKTK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000111">
        <property type="term" value="C:nucleotide-excision repair factor 2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071942">
        <property type="term" value="C:XPC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.20.110">
        <property type="entry name" value="Rad4, beta-hairpin domain BHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2460">
        <property type="entry name" value="Rad4, beta-hairpin domain BHD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.260.10">
        <property type="entry name" value="Transglutaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004583">
        <property type="entry name" value="DNA_repair_Rad4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018325">
        <property type="entry name" value="Rad4/PNGase_transGLS-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018326">
        <property type="entry name" value="Rad4_beta-hairpin_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018328">
        <property type="entry name" value="Rad4_beta-hairpin_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042488">
        <property type="entry name" value="Rad4_BHD3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036985">
        <property type="entry name" value="Transglutaminase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12135:SF2">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12135">
        <property type="entry name" value="DNA REPAIR PROTEIN XP-C / RAD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10403">
        <property type="entry name" value="BHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10405">
        <property type="entry name" value="BHD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03835">
        <property type="entry name" value="Rad4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01030">
        <property type="entry name" value="BHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01032">
        <property type="entry name" value="BHD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad4 beta-hairpin" evidence="2">
        <location>
            <begin position="574"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="domain" description="Rad4 beta-hairpin" evidence="3">
        <location>
            <begin position="673"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="391"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="789"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="874"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="133"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="396"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="933"/>
            <end position="949"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ82797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="971" mass="107496" checksum="2DC42F36A1CAFA5D" modified="2010-06-15" version="1">MPARRKGRKVPQKEKLKNACKKEAPVVAMTTRKRKAMFPSLADDSADGSSSPSDGGGGGGGGDGSDVGVCVSGRKKGKGKGKGKGKGKAIAQVAIDSGSDEDQEEEESEADDEEDDMQWEDVLNVISAPEAAGTTGTGSSSTPNPKRDLELTLNRGKTELSISSGKKKKGASAAERQIRLVTHCLHVQYLVFHGSIRNRWLCDPELQAILLSHLSPSLLLDVERLQKKQGKHSAASKSPGVTDPSLQVLDGLMHWWKKRFTVTRPGLRKNGYKPMSQFVIERDEVLANRLQGLSQGEDGGFRFTTGRTDDHGEPTYTIFKNEGERVESLREFREMARECKGSKDVGAMFFTALLRALGLEARMVLSLQPLGFGFTERETFSERVLAVADGNGGGGRVEKSEEVVGEKKTKGNEPPEIEPTTTIDRDLPYPIFWTEVYSPTTSTWITIDPLVLTALGSAPDLLSKFEPKGKNAVESKRVIAYVLAYSDNGTAKDVTVRYLAKNSFPGKAKGFRILPSEVPVYDIEGNIVASYKRDWFGNVMRGYQARGEPKLERDVKEDEELVGIAATETKSFAENGKESIGWYKDHPEYILERHLKRDEAIKPGKSHVKIFVSGKGEKAKNEKVYARQDMVSCKSVENWYREGRVIKEGEQALKKRPYCKGFIFWDRQSMWSWVRLSMGRFLGIRSARVAKKLKISYADAVIGFEFKHQRAAPYIDGIVVAAENEDIVRDAWEEEQVQRKIKEDGKREKAALTLWRRFIVGLRIVQRLHEVYEDGDGVEEANPFTAKKFRGKGQPAHGAHDEGEEGGGFINDDASDGGEPGGFIRDNQDEDIEMGVGGGFLLDVEEDPSEKVLVKKNSIPTQAMSMKEMLAREQEAAQSIFDSEEEAEDTASSQEAKSSYFVNQTPPTTRKKAGKKELVVTVVVKKPAGSAKKNPKRKVAPKTGTRSKHFVHEPESAPEGFTPRRSLRNRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>D5GE74</accession>
    <name>D5GE74_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA polymerase II holoenzyme cyclin-like subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00006408001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family. Cyclin C subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430164">
        <property type="protein sequence ID" value="CAZ82817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838626.1">
        <property type="nucleotide sequence ID" value="XM_002838580.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GE74"/>
    <dbReference type="FunCoup" id="D5GE74">
        <property type="interactions" value="87"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GE74"/>
    <dbReference type="GeneID" id="9185473"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006408001"/>
    <dbReference type="eggNOG" id="KOG0834">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038278_1_0_1"/>
    <dbReference type="InParanoid" id="D5GE74"/>
    <dbReference type="OMA" id="NACAWIM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20546">
        <property type="entry name" value="CYCLIN_SpCG1C_ScCTK2-like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043198">
        <property type="entry name" value="Cyclin/Ssn8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10026">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0195">Cyclin</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="5">
        <location>
            <begin position="80"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="5">
        <location>
            <begin position="194"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="305"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="57"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="308"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="371"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ82817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="405" mass="46181" checksum="CFAE89B4D74851A2" modified="2010-06-15" version="1">MGRIPNDRLRIAQSPPEMHSTQPAPSRRSTSPSSTHSQNFSNTYIQRSRPYLTDQQIEERSRRDSQTEAKETSTRLNACAWIMQLGHALQFPIRTMATAMILYHRSRLFSRNPYSEQQYVDVAVAALFVACKIEDTLKKSREILATSYNMRHPQHEPINSDSSILDETVKRIIGIERVILESSSFDFRYRHAQPFLIKFAKKFGCSKALTQLAWDISVDVYKTLSPLKATPHVLALASLDLAMRLEDQRVEIEYEKFEASREVVLSVIDDLLELYTSHKIQTIVGLNYDSNVYLTQRIQLNKERTPYTNGHSSQNGTSSNSSSHQNNHSNNNHYIHPSRDFLNTSPAESIPPTVVPTERGTYGTIRFMIDPARERTEKEQMNDSGRSKEFASPNGVVVSPWSGRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>D5GED4</accession>
    <name>D5GED4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00006433001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430175">
        <property type="protein sequence ID" value="CAZ82877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838686.1">
        <property type="nucleotide sequence ID" value="XM_002838640.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GED4"/>
    <dbReference type="STRING" id="656061.D5GED4"/>
    <dbReference type="GeneID" id="9184881"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006433001"/>
    <dbReference type="eggNOG" id="ENOG502S39H">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_953737_0_0_1"/>
    <dbReference type="InParanoid" id="D5GED4"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016308">
        <property type="term" value="F:1-phosphatidylinositol-4-phosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.800.10">
        <property type="entry name" value="Phosphatidylinositol Phosphate Kinase II Beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002498">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027484">
        <property type="entry name" value="PInositol-4-P-5-kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023610">
        <property type="entry name" value="PInositol-4/5-P-5/4-kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086:SF126">
        <property type="entry name" value="PIPK DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01504">
        <property type="entry name" value="PIP5K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIPK" evidence="1">
        <location>
            <begin position="136"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="292" mass="34010" checksum="2988BFD5A5BA5BCB" modified="2010-06-15" version="1">MADHSKPNSSGRERGGPQSYDWAHMSNRGFFARLVLSFFSTKYGIDMTIHCLLEPFRYSLASADELQIQAEETDYLFGLPHNSQWRSPEMKWHFNLETFFSLYYAYFHRAAPLLFYHLRKDAFKLDEEYYQSQFTKKLRPVDGLGFSGSLFFYSDDKSFIVKSIGRRFEYKFLYSTLMHPLGMYYRERPGTLLSEITDVLYTFDFRLGGCLGVSPSHYIVMTNALEGLDKERGCRKWDLKPQGFFEPTRDLVPDDMKARAAKSGLADGLDEEIVLTRKQKAHFMYALPLFAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>D5GER7</accession>
    <name>D5GER7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00001345001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430185">
        <property type="protein sequence ID" value="CAZ83010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838819.1">
        <property type="nucleotide sequence ID" value="XM_002838773.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GER7"/>
    <dbReference type="STRING" id="656061.D5GER7"/>
    <dbReference type="GeneID" id="9184922"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001345001"/>
    <dbReference type="eggNOG" id="ENOG502RXTJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044092_0_0_1"/>
    <dbReference type="InParanoid" id="D5GER7"/>
    <dbReference type="OMA" id="SIHMIHG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40129">
        <property type="entry name" value="KETOPANTOATE REDUCTASE N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40129:SF2">
        <property type="entry name" value="KETOPANTOATE REDUCTASE N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ83010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="281" mass="31142" checksum="E10D779313036513" modified="2010-06-15" version="1">MSADREDPVQVLILGAGWTSTFLIPLLEDNKITYAATTRKGDDNSIPFEFDPQSDDPTPFLPLPSAKTVLIAFPIKGTGGSKLLVTLYMRTHQHVKTNFILLGTTGIWPDKGLHDRFTIPFPDNPRKIAEDELLSLGGAVLNLAGLWGGSRDPRNWVGRVAPDKEALAGKGSLHLIHGWDVARSIIAVHKAFTPGERWLITDLRIYDWWDLASAWDEGGLLAVPKVETTDGKQAEWVQELMVEKGIKSLPRPPEQLKRGLNSLHFWKHFGIMPSRSLVSGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>D5GF11</accession>
    <name>D5GF11_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00006690001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430197">
        <property type="protein sequence ID" value="CAZ83104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838913.1">
        <property type="nucleotide sequence ID" value="XM_002838867.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GF11"/>
    <dbReference type="STRING" id="656061.D5GF11"/>
    <dbReference type="GeneID" id="9188642"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006690001"/>
    <dbReference type="eggNOG" id="KOG2515">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025462_0_2_1"/>
    <dbReference type="InParanoid" id="D5GF11"/>
    <dbReference type="OMA" id="TMGIRNM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021848">
        <property type="entry name" value="HODM_asu-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11927">
        <property type="entry name" value="HODM_asu-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ83104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="405" mass="46003" checksum="9E9AA91BB016EB55" modified="2010-06-15" version="1">MALLPSVQDHLLHHLPEYLLLTASLSSFVVLILGWRRARGDGAPIRPSPVEKGPAREPGVWIPLDFESPTPPPYPDWNISTTKPLPYRPFRYGPKYSITMGLRKMPFEEWIELDNQFPAYHSIKVSRILSRGARASTTNPVAYDAAVELLEHLVDYLPARYPSLYTRTGAGMHNAYTGETFDITATPLREDPVQMAARMVQDDLVIMIEGKDGQYYLQAGSILLAGFWRLEDKLGMPLDEIHTTGDVPGYREKLAKGMNNFFARVMPGGPVIRNNYFMQADDCLAWSHSIGSEDGGQIGWYTSEANKGIEHYHFRSERQTLRRLPKSGGVVFTIRTYFLPITEMAKEPYAPGRLASAIRSWGDDVAKYKGRERWQGVLLAYLDKEHERQMAEGLEPEKEEYVYPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFW2</accession>
    <name>D5GFW2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="12">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00007113001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430220">
        <property type="protein sequence ID" value="CAZ83405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839214.1">
        <property type="nucleotide sequence ID" value="XM_002839168.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFW2"/>
    <dbReference type="FunCoup" id="D5GFW2">
        <property type="interactions" value="248"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GFW2"/>
    <dbReference type="GeneID" id="9181750"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007113001"/>
    <dbReference type="eggNOG" id="KOG1483">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013430_4_3_1"/>
    <dbReference type="InParanoid" id="D5GFW2"/>
    <dbReference type="OMA" id="CLFHQHG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006882">
        <property type="term" value="P:intracellular zinc ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1510.10:FF:000024">
        <property type="entry name" value="Solute carrier family 30 (Zinc transporter), member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027470">
        <property type="entry name" value="Cation_efflux_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036837">
        <property type="entry name" value="Cation_efflux_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45820">
        <property type="entry name" value="FI23527P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45820:SF4">
        <property type="entry name" value="ZINC TRANSPORTER 63C, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16916">
        <property type="entry name" value="ZT_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160240">
        <property type="entry name" value="Cation efflux protein cytoplasmic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="76"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="229"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="265"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="10">
        <location>
            <begin position="10"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein cytoplasmic" evidence="11">
        <location>
            <begin position="295"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="140"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="162"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="200"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ83405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="425" mass="46128" checksum="187CCA5095A95B78" modified="2010-06-15" version="1">MAFSRSARIITLLVIDSLFFLLEIIVGYSVHSLALVADSFHMLNDVFSLLVALWAIKLARQKSTSSYTYGWQRAEVLGALINGVFLLALCLSIFLEAIQRFFEPQEISTPWLVLGVGSAGLASNILGLFLFHDHGHSHGGNSHEHDLESSLVGEESAAAGHEHHKHTRGLRGHIHDPAEDDRGDIDDILPDIVGRKRAYSRSCHQNHNHAKPKENKSSHSHQNLNMRGVFLHVLGDALGNVGVMSVAGALLLLPETIWWRHLLDPSISLLITMIIFSSALPLCKSASKILLQGVPKGISLEEVKEDIASIQGVESVHELHIWQLSDVKMIASLHIQIAFDPECEGGGRYMQLANAVRTCLHAYGIHSSTIQPEYTKEEEAAASRTGSTRGGTEEEAACLLECGDGCVDGKCCAPGSGAPGDEVIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFW3</accession>
    <name>D5GFW3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00007114001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430220">
        <property type="protein sequence ID" value="CAZ83406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839215.1">
        <property type="nucleotide sequence ID" value="XM_002839169.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFW3"/>
    <dbReference type="FunCoup" id="D5GFW3">
        <property type="interactions" value="503"/>
    </dbReference>
    <dbReference type="GeneID" id="9181751"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007114001"/>
    <dbReference type="eggNOG" id="KOG3662">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011607_0_0_1"/>
    <dbReference type="InParanoid" id="D5GFW3"/>
    <dbReference type="OMA" id="MARHIEY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033308">
        <property type="entry name" value="PGAP5/Cdc1/Ted1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13315">
        <property type="entry name" value="METALLO PHOSPHOESTERASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13315:SF4">
        <property type="entry name" value="METALLOPHOSPHOESTERASE, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="440"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="522"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="6">
        <location>
            <begin position="133"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ83406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="548" mass="62823" checksum="35048B612CFE54A3" modified="2010-06-15" version="1">MSSPSSVHSLYRPSSISITMVPARNRGFLANLRAYYYRGGKRKEWKSTLLSIRRLVEAAWIVVLWWGERRVFRSAIEACQWDRWEDWPEGAMPHRAVLIADPQLVDPHTYPRRGPFLSATIFYTDQYMSRSYETIQERLSPSTTVFLGDLFDGGREWTHEQSSRYAQNHHLHEDLIPITEDPRGAKDWKDYGDSYWMGEYRRFLKIFPSWPYRRTVKTLPGNHDFGMGNGIREGVKDRFRTYFGETSGVLEAGNHTIVLIDSVSLSNDNNPKIYLPARDFLDSLPDLLTTPSPTLLPHVIEEAANPLSSPSGQDSIPQNPTILLTHVPLYRPADTPCGPHRESKDPILIHAGYQYQNVLQPALSLEILQKTNAKYVFSGDDHDYCEVEHAGNIKEVTVKSFSWAMGVRRPGFYMVSLYTGNASPDTESVQGRLCLLPDQWGVFTVYTFMLLVTILAATFDFRRSRRAALNYRGNVLPISKGGSDISSAYRQSSAKNNDWGDGEDGNFKYSKRKQYSTGWKAWVVYVTREVLLVAGVVFVWYTWLIWRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>D5GG76</accession>
    <name>D5GG76_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00007246001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430242">
        <property type="protein sequence ID" value="CAZ83519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839328.1">
        <property type="nucleotide sequence ID" value="XM_002839282.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GG76"/>
    <dbReference type="STRING" id="656061.D5GG76"/>
    <dbReference type="GeneID" id="9184581"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007246001"/>
    <dbReference type="eggNOG" id="KOG0269">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010233_1_0_1"/>
    <dbReference type="InParanoid" id="D5GG76"/>
    <dbReference type="OMA" id="RRVLWRP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061700">
        <property type="term" value="C:GATOR2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016239">
        <property type="term" value="P:positive regulation of macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037590">
        <property type="entry name" value="WDR24"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46200">
        <property type="entry name" value="GATOR COMPLEX PROTEIN WDR24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46200:SF1">
        <property type="entry name" value="GATOR COMPLEX PROTEIN WDR24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 6" id="KW-0853">WD repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="198"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="285"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="437"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="595"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="777"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="24"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="34"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="639"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="659"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="820"/>
            <end position="836"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ83519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1073" mass="115563" checksum="5BA5534AC9934A19" modified="2010-06-15" version="1">MADPFNPHYHSQGLTHHHPHHPHTSNLHPSIQSPAPPPPPPPPPPTPAPAPAVTHSRAPKAVFARLTQPFIGTSRPPSSSGPGSGLHGNLPTSAPDCNNFLLLRENRYNLNSPIFALSANIEGDQVVIAGRGILRILRVSYDEIAEASVLRVPAEQKKHPQYDVKWGTVHQKNTVATAGTNGTICVYDTKDGMLDRQLREHGRQVHKVAFNPADGRLLLSASQDGTVKLWDLREKKSRLTFVGRADAVRDVQFNAGNAVEFAAAFDNGTIQRWDYRKDNTYERSLSAHNGPAFTVDWHPDGKHCASGGRDRTVKVWDFYADPRQKAKHTISTMTSVSRISWRPLKQVTTELATCAIGNDHRVHVWDLKRPYIPTRIMDEHENAVTGILWKDEDTLWSCSKDNMFVQNDVSFASRPINSLTHGAFSWSPTGSFTFTVEQRKSRRSTSRGFDSEDDLAGHDRRRHGRSSSFRSSKPTLGGLETLDKIFVPSQASASVHIPGLFDEVAFSYLAQNYIFDLEGVMGGSKISLGEACEANARTAWMMQKYRTAQTWKVLQLALVREEKAIERRHEAKATGLSPATGVASSLMMRKQALAAAEQSNSGGATPLAGPTHIGIATPTPGRPDGSVDEALLLLPPAFGTSLGSSTTSTDGEEYTGGHVNDDETEGAKHQPPLDVTTDAMVGGEKPILTDSQNGVVNTTTANINNYNTTSATTATTIGSLALFSESTGHSPATPIPPVPPIPMYQSAGSPAVHMLHSEQYSSSASGNMEKRRERFYSLASETTTTSSSSYGDFRNRVSEDDDESTSPKTGTWMEAIVEEPPLPPPPPPPQQPPASAPPSLHLPSAKNKASQATQGKEEDQTFTLHPLPLLETLLEYYASTTDVQMHTTIFLLLSSSQTFSPSPLLKALFNTPTSTPPTTSSPTTAAITATPPLSLLESISSYLDLLRRLNLHSVAASVIKCAQALPEFTALGQTNTHLDFACGRCARPIAIATATATNTATSMKVGAGGGGLGCGKCGAVDGCVICWGKVGRWSMCQGCGHGGHDGCLREWFLGGGTRGECAAVGCGHYCLPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5GG90</accession>
    <name>D5GG90_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00007264001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430253">
        <property type="protein sequence ID" value="CAZ83533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839342.1">
        <property type="nucleotide sequence ID" value="XM_002839296.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GG90"/>
    <dbReference type="STRING" id="656061.D5GG90"/>
    <dbReference type="GeneID" id="9184595"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007264001"/>
    <dbReference type="eggNOG" id="KOG2659">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073203_0_0_1"/>
    <dbReference type="InParanoid" id="D5GG90"/>
    <dbReference type="OMA" id="KMILWAQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013144">
        <property type="entry name" value="CRA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024964">
        <property type="entry name" value="CTLH/CRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006595">
        <property type="entry name" value="CTLH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050618">
        <property type="entry name" value="Ubq-SigPath_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12864">
        <property type="entry name" value="RAN BINDING PROTEIN 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10607">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00757">
        <property type="entry name" value="CRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00668">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50897">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CTLH" evidence="2">
        <location>
            <begin position="30"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ83533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="207" mass="23412" checksum="234710A164865674" modified="2010-06-15" version="1">MNYLIIEGYKSAAVNFAQEANMSHQVDLDSSQERVDIRHAIHHGDIQTVIERINELHPELLETNLPLHFSLLRLQLIELIRNCAQSPDGDISEALAFATTHLAPRAPGNSKYLQDLERTMALLCFPMENLAPPLAELMDPALRRQVADKVNEAILEVQGVPKEAKIRRLVRLRAWAEQRMRSERRDMPNMDLGLDVESQTSDDAMGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGL0</accession>
    <name>D5GGL0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007543001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430275">
        <property type="protein sequence ID" value="CAZ83740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839549.1">
        <property type="nucleotide sequence ID" value="XM_002839503.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGL0"/>
    <dbReference type="FunCoup" id="D5GGL0">
        <property type="interactions" value="43"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GGL0"/>
    <dbReference type="GeneID" id="9181710"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007543001"/>
    <dbReference type="eggNOG" id="KOG0504">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1373103_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGL0"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="199" mass="21410" checksum="29236EE017495120" modified="2010-06-15" version="1">MTHYLQRSTRKSDLPIPLDYRVIDVLLELCRKGELKELQELLFTLANEAPPSVLPLDLLTASKGKESLNNTLHMASANGHYEVARYLLTLLPRPTPTNPNPHKPHAFPTVTNNAGHNVLFEAQSANKEDVAKFLLETCPELAAPIGRTEEVVMEGEGEGEGEGGAAEKGKGKETEGVTGGMGELEISAGDTKTNKAGNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGX3</accession>
    <name>D5GGX3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007582001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430286">
        <property type="protein sequence ID" value="CAZ83766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839575.1">
        <property type="nucleotide sequence ID" value="XM_002839529.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGX3"/>
    <dbReference type="GeneID" id="9181737"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007582001"/>
    <dbReference type="HOGENOM" id="CLU_1138711_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGX3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="174"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="181"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="219"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="244" mass="27194" checksum="A7766DD35996BFE9" modified="2010-06-15" version="1">MVGYGRWLVIILAPRNKCPETDSITIIILPRGFLTCYTNRDPGFLPITYCHVPFHQASLSPQYYPPSPPEAQVLMRRMEEMEEKWKKKGKNKVPGNKCREFPWDGADHLVACTPPLADHSHGSWPYVGEENTKEYFAATGKEMGNHCSCSESIREMRTEIGELRKDIAKLTRDRAVPKAQVGGGRTTARSGGGGGGSGGVYGQATVATRRRWPVGRYGSSEESKAAEEFVARARRSRNYGRDRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGY1</accession>
    <name>D5GGY1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GCS light chain</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Gamma-ECS regulatory subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Gamma-glutamylcysteine synthetase regulatory subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Glutamate--cysteine ligase modifier subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00007611001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterodimer of a catalytic heavy chain and a regulatory light chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the aldo/keto reductase family. Glutamate--cysteine ligase light chain subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430297">
        <property type="protein sequence ID" value="CAZ83774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839583.1">
        <property type="nucleotide sequence ID" value="XM_002839537.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGY1"/>
    <dbReference type="STRING" id="656061.D5GGY1"/>
    <dbReference type="GeneID" id="9185028"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007611001"/>
    <dbReference type="eggNOG" id="KOG3023">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055657_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGY1"/>
    <dbReference type="OMA" id="CTDILPR"/>
    <dbReference type="UniPathway" id="UPA00142">
        <property type="reaction ID" value="UER00209"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017109">
        <property type="term" value="C:glutamate-cysteine ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035226">
        <property type="term" value="F:glutamate-cysteine ligase catalytic subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006750">
        <property type="term" value="P:glutathione biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032963">
        <property type="entry name" value="Gclm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13295">
        <property type="entry name" value="GLUTAMATE CYSTEINE LIGASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13295:SF4">
        <property type="entry name" value="GLUTAMATE--CYSTEINE LIGASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0317">Glutathione biosynthesis</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="10">
        <location>
            <begin position="93"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ83774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="288" mass="31883" checksum="3AE87B444674C90E" modified="2010-06-15" version="1">MASRCILSTGNVMTAGAQVIRKPTLEKSNTELVNSLRENFKEASSTLPLSTNPNIAATSNPETRLAPEEWTKLGSDGVLYVPTESGEPHGLAEDREEYDVTVKLFYLPGVPSSSRCRHTEEAVRLVCKELGISSIDLLIVSFPNISFDAEDEDISSEEDVSEWVRTYRTLEGLHSAKKISKIGLAEFGATRLSQLLPQTNVKPSVDQINVRDCCVVPKPLILYAKERGIELLTHNDCTDILPVDTLRQLVVDEFGLFRADAKLVPQWVVKYTAVIRSRGVVENKGRVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>D5GH30</accession>
    <name>D5GH30_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007680001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430297">
        <property type="protein sequence ID" value="CAZ83823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839632.1">
        <property type="nucleotide sequence ID" value="XM_002839586.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GH30"/>
    <dbReference type="STRING" id="656061.D5GH30"/>
    <dbReference type="GeneID" id="9185077"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007680001"/>
    <dbReference type="HOGENOM" id="CLU_357949_0_0_1"/>
    <dbReference type="InParanoid" id="D5GH30"/>
    <dbReference type="OMA" id="PAIEYEY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40903">
        <property type="entry name" value="GLYCINE-RICH CELL WALL STRUCTURAL PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40903:SF1">
        <property type="entry name" value="HYPHALLY REGULATED CELL WALL PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="486"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="613"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="698"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="313"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="486"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="511"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="537"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="548"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="588"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="614"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="654"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="721"/>
            <end position="731"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="783" mass="84855" checksum="CFA11B56C4A11071" modified="2010-06-15" version="1">MSFPRPHTSQGRPGQFQDDPDPKLQFELPPFDPDHRGEFDDGPRGRDFRPQIHQDNPPRHNPVPNTSALRRASVGRGLHSEMSRGMPGAFPGGGIGGGGDGGQRGPVVIHGRLPETRYVCFKKKTSGTSWVSGRTVCEHITKDIGPEATERRIRRHENPGMGILAILSKMAYYQQQHIHLQEDRLNEAEKGPGWHWVISAVGKEKATKAFWAIYSRLPKSQKGHQHRHSIGGPLPQMPLGEPKGRPSLHQQHQHHHQRQGPDSFKVQPVQIPPPPRPSNFLSTDLPSPPGAFPQNLYGEEEPFRSGNFGGVPPLEPPRAPSRPNPNFDFSDGPRIIEVEPPIPGAFPQYQDHHQQQQQGREESFLRSNIRNHLPQGTFGAVDSYSSVPGNRSSMPAMPAMPGGLGDMGGGGMGGGMGGGMGGGMGSGMGSGMGSGMGGGMGGGMGGGMGGGMGSGMGGGMGGGMGGGMGGGMGGGMGGDMGGGMGGGMSGTGGMPNGMPGGMLGGMPNGLRGDHPPRDADIHDPRGRDSRPTSQQMPRHDPPMDPHRSNSHHRGPSSQPRRRRNSSFHNESFPPYRESHHPRRGRAATNPNRATMDSTYWDSDKMHRTDEWVDSVSNSSGVGSTSSSQDGYLGANGDYVLDTPPPARRRSISVHGERERDREYHHPLHRGRSFREPHRHSTYNTGAGRPAIEYEYAVAPRNGGSGGRPRYRTAYHGMDSRQRRRSLSRRRSGYFGDRGHEYEYESADDIANGMRDLRIDSGRGEGRRQSMYERYMDTGMVAYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5GH45</accession>
    <name>D5GH45_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00007696001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430297">
        <property type="protein sequence ID" value="CAZ83838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839647.1">
        <property type="nucleotide sequence ID" value="XM_002839601.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GH45"/>
    <dbReference type="FunCoup" id="D5GH45">
        <property type="interactions" value="49"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GH45"/>
    <dbReference type="GeneID" id="9185092"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007696001"/>
    <dbReference type="eggNOG" id="KOG0283">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004759_5_0_1"/>
    <dbReference type="InParanoid" id="D5GH45"/>
    <dbReference type="OMA" id="DTEGLKW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000697">
        <property type="entry name" value="WD repeat protein, variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040324">
        <property type="entry name" value="WDR44/Dgr2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14221">
        <property type="entry name" value="WD REPEAT DOMAIN 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14221:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="270"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="328"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="368"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="523"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="114"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="600"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="718"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="851"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="39"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="57"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="620"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="741"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="766"/>
            <end position="778"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ83838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="914" mass="98602" checksum="69C534FCA1A25CB1" modified="2010-06-15" version="1">MTSTSILPLVNISKSRATENPAQNTPSHSPITPTSDLVSGNGRSTSSTGGDGPNALSASGSIPSSFSRPSTIPAPTADPEKLQSPNIKNTGIGLDNTVPFDPLAMQILRRTGTENTLRQKLRKDSYDDSMARNPGESVGGIGERNAPDATARNTDAQPQGREKKKGVSFLSRIMGGKKKGGDMPNDEDTLEGDVRTEGMDAHVFSQPIGYIPQFPAPPKYIRVRSHNKPKRDFDQTFLAQELYRRPVYEENGKLVAGSTADVSLLSLALPKHKSGAIWAMKFSKDGRYLAAGGQDRIVRVWQVIGIYSSGGGVRLNAPVFLSEPIHEYAGHTADVLDLSWSKNNFLLSSSMDKTVRLWHVSRKECLCAFQHSDFVTAIVFHPRDDRFFLAGSLDSKLRLWSIPDKSVAFWNELPDLITAVAFTPDGRMAIAGCLSGLCLFYETEGLRYNTQVHVRSSHGRNAKGSKITGIETITFPPDDPNGEVKLLITSNDSRVRMYNARDKSLELKFKGYENTCSQIHATFSDDAKYVISGSEDRRVYIWNVGLGETEKKDKRPVEYFEAHPAIVTVGVMAPAKTRQLLGSSGDPLYDLCNPPPVTLVSRSDSVSSKKEPATSAGGGKRSEGDHATSHTPKAPEESPAYVARSAHSDGNIIVTADYTGRIKVFRQDCAHVKRKNESWETSSTFSKKIGNGVFGGRRNSLSHGSDRILSWRQSIASSGSLDDSIGGGRFGDGSSSRNRSISPRKSTGALSIGSNGVTGRFARSGSVRSRTTSIANDTVHQRNSDGGTANVRSPGRSALGGGDRPGSSATMPVTGDDRMTQEASANLAYYNPKHMPTSGASAATTRATAGLRQGSRMTTGSDDSADEVFSTDGEGDESENETVRCKRCGGTSFKAKQSRKGEHKLLCVKCNSVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHJ0</accession>
    <name>D5GHJ0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00007938001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430319">
        <property type="protein sequence ID" value="CAZ83983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839792.1">
        <property type="nucleotide sequence ID" value="XM_002839746.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHJ0"/>
    <dbReference type="GeneID" id="9183637"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007938001"/>
    <dbReference type="eggNOG" id="KOG1237">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004790_4_2_1"/>
    <dbReference type="InParanoid" id="D5GHJ0"/>
    <dbReference type="OMA" id="LVLHIIV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071916">
        <property type="term" value="F:dipeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000085">
        <property type="entry name" value="MFS peptide transporter Ptr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018456">
        <property type="entry name" value="PTR2_symporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01023">
        <property type="entry name" value="PTR2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="184"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="212"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="296"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="452"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="488"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="524"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="550"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="34"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ83983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="623" mass="68176" checksum="95C462827020CF43" modified="2010-06-15" version="1">MNAPRDPHEAPLPGTDATAVEPIAKRAEMVVGRTASAHAAREKQSVNSNDLETGAGGFADDSGGVILRREELPRGGLADDEIEPTPEELATLRRVSDSLPISAYYVVVVELCERFSYYGCNAPLQNYISYPKEGNKKNGQPGALGLGQQGATALTNFFHFWAYLTPIVGAVIADQYLGRYRTICYGCGLYIAGLFLLFMTALPASINGNHALGGLIPSLIIIGTATGIIKSNVSPLTVEQYRKTRRFVKTLETGEKVIVDPVVTAQTIFNWFYWAINVGALSPLATVFSEKKVGFWFAYLLPFLMFFVGVGALVAGRNKYIRRPPEGSVLPNAFRVVSIGIRYRSLEAAKPSNLEAKGLLHKYNVRWTDHFVDEVRRGLVACKVFVAYPFYSICANQGSNNLVSQAGAMKLGGTPNDLISKLNPLTLIIFVPIFDRVVFPMLRRAGIHFRPITRITLGFFIQSLSIVYITVLQHYIYKSPPNSINVWIQGPCYVLGAISEIFTYITALEYAFTHAPASMKSVVTSVYFFHNAIAAVIGISLTPVNKDPEILWTYASVASGAAVAGIIFWFSFRHLNEQEDEMMDLESKDIGRGSNGISSAAQGDCTGSTFLGEANIRGPTKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHL3</accession>
    <name>D5GHL3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="10">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">GSTUM_00007972001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430319">
        <property type="protein sequence ID" value="CAZ84006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839815.1">
        <property type="nucleotide sequence ID" value="XM_002839769.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHL3"/>
    <dbReference type="STRING" id="656061.D5GHL3"/>
    <dbReference type="GeneID" id="9183660"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007972001"/>
    <dbReference type="eggNOG" id="KOG0141">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_4_1_1"/>
    <dbReference type="InParanoid" id="D5GHL3"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050741">
        <property type="entry name" value="Acyl-CoA_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48083:SF15">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE APDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48083">
        <property type="entry name" value="MEDIUM-CHAIN SPECIFIC ACYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0274">FAD</keyword>
    <keyword evidence="3 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="9">
        <location>
            <begin position="71"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="8">
        <location>
            <begin position="202"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="7">
        <location>
            <begin position="310"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="CAZ84006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="381" mass="41726" checksum="8D0E25727D60F4D8" modified="2010-06-15" version="1">MCKSSLNSSVDSLAVMKYDPGLGFLRKQQDPSKQNIQANNLTLSVTLAAVLPFSEPPYPNGLPSPYYAPLHHRWQKACCAFISENLTANALEWEREELVPPEIFSKFAAANFLLPSLPAPLLIEWAKQLGLDILARGLKVEEFDYLHFLIYSDEMTGNGLSGPGASLTTGITFGVPPILKFGSKQLLERFIPDLLMGRARSCIVITEPGTGSDVAGIERTAVKSADEKEYIVNGSKKWITNGIWADFVTTAVLTGGPWPSGLSLLLIPLKNNLSIIIRKLVIMGLAASGTTYIEFDDTHVPVENLIGIEGMGIRCIMTNFNHELLTTAIGVTRQARVAVSTALEYVLKREAFGKALVEQPVVRHRLAKVGAMVESMWAWVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHP1</accession>
    <name>D5GHP1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00008070001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430320">
        <property type="protein sequence ID" value="CAZ84071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839880.1">
        <property type="nucleotide sequence ID" value="XM_002839834.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHP1"/>
    <dbReference type="GeneID" id="9183725"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008070001"/>
    <dbReference type="HOGENOM" id="CLU_1385079_0_0_1"/>
    <dbReference type="InParanoid" id="D5GHP1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ84071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="197" mass="21942" checksum="C2E21951AA8CE49E" modified="2010-06-15" version="1">MGTLTLREFRELLEAVTPVLYKYRTGREDASNLLVSPSPPSVTHTHSPPRMDFDLPCSSWPVLFCFLIPAALGRPWGIRGTYGYSGTGLGEAAPSVKLISPVITARMKLTGRYGISDQKAMSSYPSDVFQYNPPISTPFSAAHVFSLSYLFYRILISIQDQPLLFHHYFPSLLLYDILHQILVQVSGSPIIFLPPHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHS3</accession>
    <name>D5GHS3_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00008014001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the proteasome complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peptidase T1B family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430320">
        <property type="protein sequence ID" value="CAZ84034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839843.1">
        <property type="nucleotide sequence ID" value="XM_002839797.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GHS3"/>
    <dbReference type="FunCoup" id="D5GHS3">
        <property type="interactions" value="1019"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GHS3"/>
    <dbReference type="MEROPS" id="T01.984"/>
    <dbReference type="GeneID" id="9183688"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008014001"/>
    <dbReference type="eggNOG" id="KOG0177">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035750_12_0_1"/>
    <dbReference type="InParanoid" id="D5GHS3"/>
    <dbReference type="OMA" id="MKRDHDK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061133">
        <property type="term" value="F:endopeptidase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03758">
        <property type="entry name" value="proteasome_beta_type_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000008">
        <property type="entry name" value="Proteasome subunit beta type-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035206">
        <property type="entry name" value="Proteasome_beta2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF2">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA TYPE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 5" id="KW-0647">Proteasome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ84034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="175" mass="19749" checksum="943F730C34745F40" modified="2010-06-15" version="1">MRGVTILKAEDDKTRTLNPHTLMAFSGEAGDTVQFAEYIQANVQLYGMRNNSKWQLSPSAVASFVRRQLAKSLRSRNPYYVNLLIGGFDCTTGKPHLYWLDYLASSAAVPYAAHGYAQYYCLSILDKHHHPDITLEEGIKLMRMCHDELIKRLPIDFKGLTVKAVTENGVEVVQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIF7</accession>
    <name>D5GIF7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00008477001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430326">
        <property type="protein sequence ID" value="CAZ84300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840109.1">
        <property type="nucleotide sequence ID" value="XM_002840063.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIF7"/>
    <dbReference type="FunCoup" id="D5GIF7">
        <property type="interactions" value="31"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GIF7"/>
    <dbReference type="GeneID" id="9184658"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008477001"/>
    <dbReference type="eggNOG" id="ENOG502QRN9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092544_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIF7"/>
    <dbReference type="OMA" id="TYNDVHN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052657">
        <property type="term" value="F:guanine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004422">
        <property type="term" value="F:hypoxanthine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000310">
        <property type="term" value="F:xanthine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032263">
        <property type="term" value="P:GMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046100">
        <property type="term" value="P:hypoxanthine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032264">
        <property type="term" value="P:IMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032265">
        <property type="term" value="P:XMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000033">
        <property type="entry name" value="Xanthine phosphoribosyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43363">
        <property type="entry name" value="HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43363:SF1">
        <property type="entry name" value="HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ84300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="215" mass="23909" checksum="3116FC68D159FCE0" modified="2010-06-15" version="1">MVERKYITYNQVHKLCQNAAPKILDEFKPNLMIAIGGGGYVPARILRSFLKTPGSPNIPIQAIGLSLYEKLSEEGPEEEIGTKVTRTQWLDFTSLSNTDLIGKNILIVDEVDDTRTTLQYAVQELSKDVQLQAEKLSGNQPKTNFFIFVLHNKDKSKGGKLPEEMVKEGRYLAAETVGDVWCCYPWEAIDIDEHDRLAAAGEAAAQKNGTNGTNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIG5</accession>
    <name>D5GIG5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00008489001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430326">
        <property type="protein sequence ID" value="CAZ84308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840117.1">
        <property type="nucleotide sequence ID" value="XM_002840071.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIG5"/>
    <dbReference type="STRING" id="656061.D5GIG5"/>
    <dbReference type="GeneID" id="9184666"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008489001"/>
    <dbReference type="eggNOG" id="ENOG502QQ04">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037759_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIG5"/>
    <dbReference type="OMA" id="CGKWWCE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ84308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="387" mass="42190" checksum="2BD04FEBCCE42671" modified="2010-06-15" version="1">MADTPLTISTILSEDLLLSIAPYLHPKDISALSLTNKLINNLLTSAQGVYRHIDFSPEKKKFQNAQKLVSPTILSATRTLVLDFTLVAIPQLESILYSSNITLLSIRGCKGIDERALMAHLSPERGTKLKGIYYFTDPAPTTSRFVQKSVTKFPNEWALVISNCKDICFDTRVCRGLKHQTDEVGHIEPRISDVKLEGGCSGCGVNLTSEGLPNVLVAPAPLHSSTIRVACKGIDGKPIADVLRCEMCVRERWCEGCGKWWCESCAVDKKHVTRPCLECGFQCGECIAAVTLFCQSCKSGYCTTHNEGADDIQCEWCKASSARRGASATASNAFLTSAAASHYAMLRLTRPGNTFVKSPAGASYKGGIGGRNFQNETIARRRALMNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIH0</accession>
    <name>D5GIH0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00008495001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thioesterase PaaI family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430326">
        <property type="protein sequence ID" value="CAZ84313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840122.1">
        <property type="nucleotide sequence ID" value="XM_002840076.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIH0"/>
    <dbReference type="STRING" id="656061.D5GIH0"/>
    <dbReference type="GeneID" id="9184671"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008495001"/>
    <dbReference type="eggNOG" id="KOG3328">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089876_12_2_1"/>
    <dbReference type="InParanoid" id="D5GIH0"/>
    <dbReference type="OMA" id="CCAKIMA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047617">
        <property type="term" value="F:fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03443">
        <property type="entry name" value="PaaI_thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000033">
        <property type="entry name" value="acyl-coenzyme A thioesterase 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039298">
        <property type="entry name" value="ACOT13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003736">
        <property type="entry name" value="PAAI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00369">
        <property type="entry name" value="unchar_dom_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21660:SF57">
        <property type="entry name" value="PAAI THIOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21660">
        <property type="entry name" value="THIOESTERASE SUPERFAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioesterase" evidence="3">
        <location>
            <begin position="40"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ84313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="131" mass="14082" checksum="CDDD5B9B0E6E03F2" modified="2010-06-15" version="1">MLRGCCAKIMARSQLKLINVEPGKVDFELLIEKMHTNRLGTLHGGAIASMVDLGGSLAVASKGLFSTGVSTDLNVTYLSSGGEVGHVIRGEATCDKFGKTLAYTTVLFRNKDDRLVARGSHTKFVYSFSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GIW4</accession>
    <name>D5GIW4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00008713001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840266.1">
        <property type="nucleotide sequence ID" value="XM_002840220.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GIW4"/>
    <dbReference type="GeneID" id="9185675"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008713001"/>
    <dbReference type="HOGENOM" id="CLU_2887466_0_0_1"/>
    <dbReference type="InParanoid" id="D5GIW4"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ84457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="63" mass="7088" checksum="FFBED61CFFCCD6CB" modified="2010-06-15" version="1">MQRSTNSSDSPEHRQVSQGKIRKNVKKGTVSNRSARLARTARDISAREKRLNKEHGKQGSQVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJ46</accession>
    <name>D5GJ46_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00008835001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840348.1">
        <property type="nucleotide sequence ID" value="XM_002840302.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJ46"/>
    <dbReference type="GeneID" id="9181880"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008835001"/>
    <dbReference type="HOGENOM" id="CLU_3224907_0_0_1"/>
    <dbReference type="InParanoid" id="D5GJ46"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ84539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="44" mass="5454" checksum="94EA888AF7AF01CA" modified="2010-06-15" version="1">MIPINREEYPEWHTARQATLLENPCRAFSRFWKELLRRHKISTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJ73</accession>
    <name>D5GJ73_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00008869001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Necessary for protein synthesis in mitochondria. Functions as a ribosome recycling factor in mitochondria.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RRF family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430329">
        <property type="protein sequence ID" value="CAZ84566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840375.1">
        <property type="nucleotide sequence ID" value="XM_002840329.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJ73"/>
    <dbReference type="STRING" id="656061.D5GJ73"/>
    <dbReference type="GeneID" id="9185934"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008869001"/>
    <dbReference type="eggNOG" id="ENOG502S5HY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057161_1_0_1"/>
    <dbReference type="InParanoid" id="D5GJ73"/>
    <dbReference type="OMA" id="NVICGEE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.20">
        <property type="entry name" value="Ribosome-recycling factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002661">
        <property type="entry name" value="Ribosome_recyc_fac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023584">
        <property type="entry name" value="Ribosome_recyc_fac_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036191">
        <property type="entry name" value="RRF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20982:SF3">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOME RECYCLING FACTOR PSEUDO 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20982">
        <property type="entry name" value="RIBOSOME RECYCLING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01765">
        <property type="entry name" value="RRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55194">
        <property type="entry name" value="Ribosome recycling factor, RRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosome recycling factor" evidence="5">
        <location>
            <begin position="91"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="17"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ84566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="258" mass="28541" checksum="DF30EB5866029AD0" modified="2010-06-15" version="1">MLRTPITRSMTHSLLRPIPPQFLRPLTPLPHPKTFTTTAHPQKKAAAKSSKAHPYGDDNSAQGKTRDSPFDYAAHFESLEAQCSAHIDKLRTDIAKLRAGGRNDPATLENIQVVLDRSTGASASLRDIAHVLPKGRSFVVSVYDAANVKYVIAAIQKADLNVQPMPDPKNTQLINVPLPPPTKETRSEIARMVGKAGERVMNYLRLARQDSHKLIKGLKKERPDDIRKADKQLELVMKKINAEAKKVIDDGKKGALEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJD5</accession>
    <name>D5GJD5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00008950001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430330">
        <property type="protein sequence ID" value="CAZ84628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840437.1">
        <property type="nucleotide sequence ID" value="XM_002840391.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJD5"/>
    <dbReference type="FunCoup" id="D5GJD5">
        <property type="interactions" value="833"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJD5"/>
    <dbReference type="GeneID" id="9182629"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008950001"/>
    <dbReference type="eggNOG" id="KOG0290">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013694_3_0_1"/>
    <dbReference type="InParanoid" id="D5GJD5"/>
    <dbReference type="OMA" id="YAEDSHN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045159">
        <property type="entry name" value="DCAF7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19919">
        <property type="entry name" value="WD REPEAT CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="644"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="39"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="441"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="574"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="41"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="75"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="98"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="121"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="169"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="181"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="202"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="225"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="249"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="290"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ84628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="750" mass="78436" checksum="D32164D14080FA93" modified="2010-06-15" version="1">MPLHGLQHHPRPHSNTHTPEVSGAGSALIQAAQALTGIGQPGAASSAVSQQQQLQQQQPQQLLQQPGGGGGGSLRVQTSQSNLTSNSGPGSGVTPIYSGSTTSGGALSSAATSGLGFSNRDLPSSSLDQQKKPTNLTFQLPSPTKGGNQGDLYSSPQELHYPHRPSDPVQAQQLSSSGLFSQHQPQPQQQYQAYHSSQPPSNTSSKPQPHTTATTPGSIPRINDPLQQQQSTMHRTNTNASLSHSSKYSPQLNTSSSSLSNRDNSAVSAGGDPGRKYTHPGALTPTYPPSSQHSSSHSHSGAAHLAMGLSGLTSPATSTREREETGVVGAVGNGKWMSPWSLYALDWCKWQPTNGGYGRVAIGSYAEDSHNYIQILDTRLASVSSTHSDTPPPAPTIEFSKVAEASHTYPITRILWEPAGSTKASTELLATSGDHLRLWSLPNSPSTGSSNSATPQGNSITRSNTFHTQPIQKLTPLALLSNSKSTDFTAPLTSLDWNPLSPSLIITSSIDTTCTIWDIPTLTAKTQLIAHDKEVFDVRFMSGSVDVFASCGADGSVRMFDLRSLEHSTIIYEPGVGKGSGADPNTNSKEDGGSPVGGAGASPPPLLRLAASPHDQHLIATFSQDSSLVRILDVRQPGQALIELKGHTGSVNCIEWNPTKRGIIAGGADDSLVLIWDLVNNTTSQSTGSNGTSVTQEKIPVASWKCDYEVNNISWSPTSAGASGAGGGGAGGIGDWLGVLGGRGIWGVKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJG5</accession>
    <name>D5GJG5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Translationally-controlled tumor protein homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00008989001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the TCTP family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430330">
        <property type="protein sequence ID" value="CAZ84658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840467.1">
        <property type="nucleotide sequence ID" value="XM_002840421.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5GJG5">
        <property type="interactions" value="824"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GJG5"/>
    <dbReference type="GeneID" id="9182659"/>
    <dbReference type="KEGG" id="tml:GSTUM_00008989001"/>
    <dbReference type="eggNOG" id="KOG1727">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095877_0_1_1"/>
    <dbReference type="InParanoid" id="D5GJG5"/>
    <dbReference type="OMA" id="CAMITEG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010507">
        <property type="term" value="P:negative regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007026">
        <property type="term" value="P:negative regulation of microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.10">
        <property type="entry name" value="Metal Binding Protein, Guanine Nucleotide Exchange Factor, Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011323">
        <property type="entry name" value="Mss4/transl-control_tumour"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034737">
        <property type="entry name" value="TCTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018105">
        <property type="entry name" value="Translational_control_tumour_p"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11991">
        <property type="entry name" value="TRANSLATIONALLY CONTROLLED TUMOR PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11991:SF0">
        <property type="entry name" value="TRANSLATIONALLY-CONTROLLED TUMOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00838">
        <property type="entry name" value="TCTP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01653">
        <property type="entry name" value="TCTPROTEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="Mss4-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51797">
        <property type="entry name" value="TCTP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TCTP" evidence="3">
        <location>
            <begin position="1"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00014759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ84658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="206" mass="23201" checksum="7FD17EB5087501A9" modified="2010-06-15" version="1">MIIYKDVISGDEMISDAFDMTLVHDVVYEVNCSMITVRKGADVDIGANASTEEDAEVVEDGQEVVNNIVHSFRLVQTGFDFNSYMVHIKAYLKKSQEHLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNGSKLTRGSIEGKNRQWDFYIGEDMNVDGMVVLLGYREDGSTPYAVFWKHGLKEEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GJY5</accession>
    <name>D5GJY5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00009270001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="FN430335">
        <property type="protein sequence ID" value="CAZ84828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840637.1">
        <property type="nucleotide sequence ID" value="XM_002840591.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GJY5"/>
    <dbReference type="STRING" id="656061.D5GJY5"/>
    <dbReference type="GeneID" id="9186937"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009270001"/>
    <dbReference type="eggNOG" id="ENOG502QPS4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009630_1_1_1"/>
    <dbReference type="InParanoid" id="D5GJY5"/>
    <dbReference type="OMA" id="WYMLFAV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00035">
        <property type="entry name" value="ChtBD1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd11618">
        <property type="entry name" value="ChtBD1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02851">
        <property type="entry name" value="E_set_GO_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.10">
        <property type="entry name" value="Endochitinase-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.80">
        <property type="entry name" value="Galactose oxidase/kelch, beta-propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001002">
        <property type="entry name" value="Chitin-bd_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036861">
        <property type="entry name" value="Endochitinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011043">
        <property type="entry name" value="Gal_Oxase/kelch_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037293">
        <property type="entry name" value="Gal_Oxidase_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009880">
        <property type="entry name" value="Glyoxal_oxidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015202">
        <property type="entry name" value="GO-like_E_set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32208:SF21">
        <property type="entry name" value="LOW QUALITY PROTEIN: ALDEHYDE OXIDASE GLOX-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32208">
        <property type="entry name" value="SECRETED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00187">
        <property type="entry name" value="Chitin_bind_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07250">
        <property type="entry name" value="Glyoxal_oxid_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09118">
        <property type="entry name" value="GO-like_E_set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00270">
        <property type="entry name" value="ChtBD1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50965">
        <property type="entry name" value="Galactose oxidase, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57016">
        <property type="entry name" value="Plant lectins/antimicrobial peptides"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50941">
        <property type="entry name" value="CHIT_BIND_I_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072796" evidence="5">
        <location>
            <begin position="21"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-1" evidence="6">
        <location>
            <begin position="80"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-1" evidence="6">
        <location>
            <begin position="145"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-1" evidence="6">
        <location>
            <begin position="238"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="192"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="103"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="121"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="165"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="185"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="258"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ84828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="815" mass="84562" checksum="838CB97A267D12E3" modified="2010-06-15" version="1">MVTTKTALILSALFAHGVFANIPFPDPYIECDPGAAEEKCLRGQYCSEEDELCKSPAPHELFRRIARPNPIPAPGDFSVDGRCGPANGGLICNPNSPNYVGGCCSQAGWCGNTPAYCGAGCISGCTSTSAGGGTPAPTGGAARPDGRCGTEFGGATCAGSAFGTCCSQYGWCGTGDAYCLPANGCQSGCTGTTPTTSTPPSVPPATAPTTTSSTGEPVLGAPSSTGVNPNPTGIPTTDGTCGATNAGRTCVGWDKGECCSMYGFCGNTDAHCGDGCQSGPCKAGGSPPAPGASPAPITNGGEFTIIGQSGVPAMHAGLLPNGRAIFLDKVENYTQLKLPNGQFAYSSEYNPDDNTVVPLAYNTNAFCAGGIFLANGDFVSLGGNAPLDFIDPTVTNGFDAIRYITRSSSDASFNGQNWREPGNKLASNRWYASAQILADGRIFVASGSLNGLDPTVPANNNPTYEILSAAGVSNGVNVPMDILVAHQPYYMYPFMHLLKNGELFVFTAKSSQIFNIGTNSITRQMPDLPGDFRTYPNTGGSVMFPLTSANGWNTKVMICGGGPYQDITAPTDPSCGVIAPEDANPTWEMDAMPEGRGMVEGVLLPDGSVLWLNGANRGAQGFELATNPTLAALLYEPTKAKGARWTQLASSTIPRLYHSVALLLLDGTVLVTGSNPHEMPILETRPGVAFITDFRVERFTPPYLQGAKATQRPSAMALSTKNLPANGSTFTISFNAATTTQGIKVALYYGGFVTHSVHMGHRMLFLDNTGFVAGTAAQTITVTMPPNKNVVPPGPYVVYVVADGTPSVGQFVTVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKD6</accession>
    <name>D5GKD6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00009492001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the APC3/CDC27 family.</text>
    </comment>
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        <property type="protein sequence ID" value="CAZ84979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_002840742.1"/>
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    <dbReference type="eggNOG" id="KOG1126">
        <property type="taxonomic scope" value="Eukaryota"/>
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        <property type="entry name" value="TPR-like_helical_dom_sf"/>
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        <property type="entry name" value="TPR_rpt"/>
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        <property type="entry name" value="CELL DIVISION CYCLE 16,23,27"/>
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    </dbReference>
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        <property type="entry name" value="CELL DIVISION CYCLE PROTEIN 27 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12895">
        <property type="entry name" value="ANAPC3"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 6" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
            <begin position="128"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
            <begin position="491"/>
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    </feature>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
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    </feature>
    <feature type="repeat" description="TPR" evidence="6">
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    </feature>
    <feature type="repeat" description="TPR" evidence="6">
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            <begin position="627"/>
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    </feature>
    <feature type="repeat" description="TPR" evidence="6">
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    <feature type="region of interest" description="Disordered" evidence="7">
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            <end position="441"/>
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            <end position="242"/>
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        </location>
    </feature>
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            <begin position="318"/>
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        </location>
    </feature>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="793" mass="88958" checksum="9CBF5D9A85A57686" modified="2010-06-15" version="1">MMPSIPQPHVIAHFRSLVWQSLDNDMLPTALFAAERLNAYDPKGGDSVHLLGLCLYRDGQYLAAESLTESWVRHLGCAYIYAQCCLKLGDGRESQGINALETCRRLWNGTSTWNQHSDTARRSIPDAATVHFLLGRLWHNAGDVKKSVDSYVAALKLNPFAWEAFTGLCDTGILSRNYPFEGSYANDRLGVNLRPNAVFKQSQEMLESLKMPSYPSQPSNIPATIPEESSGLNGLRNSSENLPSDPFSSNGGSSRDRSDLSYNNHPSFFNRLNEGVGSSLEAETPTSQPTGSSSHELFGGANGVNVTDKPPPVRKTRATTTEITTRKLTSRSTREVGNDGKRSAASSQESGPQAPARRSTRLTSKFTSKLGVGTERETRLGTKEREREAKKARTVANRSRSLHLTGTSASAKERQKERERENSEDINMSDATSKPTFAPGHQIDIKKQEAQLFILDLFKKAGAGYFSLSRFRCKEALHALNSLPLSQKDTPWAQSQLGRAYYEMANYIEAERCFLRVRHLSPVRTRDMEVFSTILWHQRKEVDLSYLAHELVELDRLSPEAWCALGNCFSLQRDHDQALKCFKRATQLNPKLAYAFTLQGHEHVSNEEYEKALASYRSAITADSRHYNAWYGLGKVFEKMGKFDTAEKHFRTASKINPTNAVLVCCVGMVMEKNKDFRGALHQYNIACEMSPTSALSRFKKARTLMLLKSYQPALKELRVLKDLAPDEANVHYLLGRLHKLLHDKKSAIKHFTIALNLDPKAGHLIKEAIENLDEDEEDEYSGDAEENRFLDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKM9</accession>
    <name>D5GKM9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00009648001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="CAZ85072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002840835.1"/>
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    <dbReference type="InParanoid" id="D5GKM9"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
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    </dbReference>
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        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="F-box_dom"/>
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        <property type="entry name" value="PSMD4"/>
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        <property type="entry name" value="UIM_dom"/>
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        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10223">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10223:SF2">
        <property type="entry name" value="F-BOX AND WD DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_6G11400)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00726">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="150"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="632"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="868"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="957"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1016"/>
            <end position="1105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1124"/>
            <end position="1153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="961"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="971"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1056"/>
            <end position="1066"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1140"/>
            <end position="1153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ85072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1153" mass="125287" checksum="EFE09336FFC5C152" modified="2010-06-15" version="1">MQTEHTQAFQDTAPSTEPLPTSRAPTAQHGIHSFSTAPPSLDDLSQGAGAGGVRGIGGERAGNDEAEDTGMSSNSNGLGDMTHPKGDWDTISPSKRKKDIISQYEARGTSPPNRGTGFHVKKTNWQGRLDSPISRFPNGTASILLFAQNSGLTLDISTEVLIHSLSFLDPQTLLVASLVSRRFHSLISSPDAWRSAFSRHFPTTSLDQSSSDLHESAAFSSSQDRRFFTRLSAGGSAGDLWRKEYILRTRLLRDLVKGKPHKSASHSNPMSKNSQGSVVITYNALTSPMSVSHIAAGFSPRGVRLLHASLDTITVTASDATVGKVERPPLAAAMLGTPSRPDLHQPVLGAAGFDVATDMDTVGVMDLSEDVGWVYGENVPNGKCYVHPYSSPYRCASEQGFFLRQDQVASRCLEPAITAVWITKKRTGGVLGTSEGQVGVMIGNSRGFVSIYGLSFGKEYRVTPTKMFCICPGIPIVSIKVDEDYSVKRKRQKSIWAFIVNALGEIYYLADPKVGWEIIPQTARIPTVVHSSIFQPPSKTIAREGMGKKEIEEKELLLDIEYTRLKQLWEGWGMDWFIELDWPGTNIILGRKRSSNRPSTGNEQTSKLIRYHLSYTPNIGAEEFVREVMTSTPEGTGAGTTSRSVFGGESQSQSSEKVGTIEALVQSSQLCERLEKWVATDFLFGDENVGMVTALAIDISNLALLSPGEDPGITADVPGGNARLFAVGTNMGDVFIWNIRHQAPSTGGDGTPSISLLPQRVVRTDSPRISTLALSSLYLVHGGNDGLVQAWDLLASTYSPVRSIHSRFSTRARRRLAQADAATLGLGNDALGDNQFAARCLVLDPDPTRLRGAVALGTHIRHWCFSSSSGSNTPKRSRKPRVGGRVCGTPSSDRGDIKGIIKSDSKNLLKERERENKERTELEKRYGISSGWAALSEEEMLEYARMISMETYEIEGGGSIESGNNSNTITPRDSSGATSSHRTTRGVEDTKEEIEDLELAQALRLSLERAWMETPAVPGDTLDSGIWGDASEYPDREVPPSPVVAGPGHQRRSTPHHTNSNRSSDFATPGGRPGAEREWPSISGITEKGRDKRQRHTSRTEEEDLEMAIQLSMKEEECRGIISAFENDDGQNQAIGKGKATGKGKGKGKGKGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5GKS7</accession>
    <name>D5GKS7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00009745001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430341">
        <property type="protein sequence ID" value="CAZ85120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002840929.1">
        <property type="nucleotide sequence ID" value="XM_002840883.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GKS7"/>
    <dbReference type="STRING" id="656061.D5GKS7"/>
    <dbReference type="GeneID" id="9183356"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009745001"/>
    <dbReference type="eggNOG" id="KOG1840">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_125_15_1"/>
    <dbReference type="InParanoid" id="D5GKS7"/>
    <dbReference type="OMA" id="YNEAEVM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053137">
        <property type="entry name" value="NLR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46082:SF6">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46082">
        <property type="entry name" value="ATP/GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13374">
        <property type="entry name" value="TPR_10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ85120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="755" mass="85649" checksum="15BE02CDBD961A2B" modified="2010-06-15" version="1">MHRRALAGRERNAPGPHHPDVLLVALRRAVKLLDERKYEEAEIMYQHALGVMEKWRGLDRPDILDSLNTLGETLLNLERYDEAEAMCRHALAGREKALGVDHAATLTSADNLGRTLYHQRKHEEAEMIFRSTQASRKEVLGLYHPDTLLSIASLGVTLLCQRKYGEAEIVYRQALGAMEKILGPDHPDVLDSVNTLGETLLDLEKYGEAEAMCRRALEGREKILGLDHRDTLLSANNLGVALRNQDKHHEAENMYRRALEGAEKTLGPDHCDTLMCIYNLAGALHKQRKYDEAEMMHRQALAGREKTLGLGHRHTIRNLTKLGALLYQEGKYDEAKIMYQRAVMGAKKFLGPGHPMTLKSVNHLTLLDERKYEEAEIIYRHALGTMEKALGPDHQDVLDSVNILGETLLDLKMYNEAEVMCRRALAGREKTFGPDHRDTLLTANNLGVALRNQNKHHEAEITYRRAVIGTGRTLGPDHRDTLMCVYNLAGLLQRQRKYDEAEMMHRRALAGREKTLGPGHRHTLRSLIKLGALLDERKYEEAEIIYRHALGTMEKALGPDHQDVLDSVNILGEALLDLKMYNEAEVMCRRALAGREKTFGPDHRDTLLTANNLGVALRNQNKHHEAEIMYRRAVIGTGRTLGPDHRDTLMCVYNLAGLLQRQRKYDEAEMMHRRALAGREKTLGPGHRHTLRSLIKLGAVLHEKRKYDESEIMYRRAVTEAEKSLGPNHPATLRSFNQLSMFLYARATSTLDEVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>D5GL26</accession>
    <name>D5GL26_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 5B</fullName>
            <ecNumber evidence="3">3.6.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Translation initiation factor IF-2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">GSTUM_00009928001</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.3" evidence="3"/>
    <dbReference type="EMBL" id="FN430344">
        <property type="protein sequence ID" value="CAZ85219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841028.1">
        <property type="nucleotide sequence ID" value="XM_002840982.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GL26"/>
    <dbReference type="FunCoup" id="D5GL26">
        <property type="interactions" value="433"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GL26"/>
    <dbReference type="GeneID" id="9185813"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009928001"/>
    <dbReference type="eggNOG" id="KOG1144">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002656_1_0_1"/>
    <dbReference type="InParanoid" id="D5GL26"/>
    <dbReference type="OMA" id="EFAVMLC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03703">
        <property type="entry name" value="aeIF5B_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01887">
        <property type="entry name" value="IF2_eIF5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000013">
        <property type="entry name" value="eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000026">
        <property type="entry name" value="Eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10050:FF:000002">
        <property type="entry name" value="Eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000112">
        <property type="entry name" value="Eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10050">
        <property type="entry name" value="Translation initiation factor IF- 2, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029459">
        <property type="entry name" value="EFTU-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015760">
        <property type="entry name" value="TIF_IF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023115">
        <property type="entry name" value="TIF_IF2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036925">
        <property type="entry name" value="TIF_IF2_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003078">
        <property type="entry name" value="PRK04004.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381:SF4">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR IF-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14578">
        <property type="entry name" value="GTP_EFTU_D4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11987">
        <property type="entry name" value="IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52156">
        <property type="entry name" value="Initiation factor IF2/eIF5b, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="15">
        <location>
            <begin position="481"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="59"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="125"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="207"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="217"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="314"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="324"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="374"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="404"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="431"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAZ85219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1075" mass="118954" checksum="7B70CD7DF2D4CDBF" modified="2010-06-15" version="1">MAKGKKGKKAHDDSWEDDIDTQAPVDPIAAAAATDSKPGTAQHDDIGGGGLMAAIKKNRDNKKKKGKAVKPLNDEVTEDGDDAATPPVDAKAPTEVIEIGEDDFGPVKKGGKKGKNAPAAAAPVEKTEELGEDEVRGADGEVRVKTKKEKEKEKKEREKQRKKEQAARKKQTEPTKAEEKKEEVAAKPMAPSAAVPDPAGGKKKKIPAALAALQKQQELRRKQEEEAARIAEEERKREEEEERIRQEEERAKAEAKEKKKEKEKERIERLKKEGKYMTKAEKERQRMAQLRLQQMVESGMKVEGLEGGPAVEKKKPKYEKKPRKGDGGKSKAEGDEEKKKEEERLVSERKAKEEAERRKEAEEAAAAAAKKEEEDVPDSWEEAFDQDGNIKESWDMSSEEEEVETSKKPAKEPKAAPPKDGKGPSKKSPDADEEEDSDSSDEVLVDASVAKRLAEQRKQEQHARRQKQIDEAKAAASVENLRSPICCILGHVDTGKTKLLDKIRQTNVQEGEAGGITQQIGATYFPMDAIKQKTAVVNKDGKMEFKVPGLLVIDTPGHESFTNLRSRGSSLCNIAILVVDIMHGLEPQTIESLNLLRDRKTPFIVALNKIDRLYDWKAIPNNGFQDSLAKQKKSVQNEFKDRLEKTIVAFQENGLNAALFYENKNVGKYVSFVPTSAHTGEGIPDMLKLLVTLTQQRMAGKLMYLSELESTVLEVKVIEGLGTTIDVILSNGVLREGDRIVMCGLNGPISTNIRALLTPQPLKELRIKSQYVHHKEVKAALGVKIAANDLEHAIAGSRLLVVGPDDDEEDLEMEVMSDLEQLFSKVSKSGAGVAVQASTLGSLEALLAFLKSSSIPVSTISIGPVYKRDVMRAGTMLEKHKEYAVMLCFDVKVDKEAQAYADEVGVKLFTAEIIYHLFDAFTAYKKEMSEQRKRESAANAVFPCILNPVAVFNKKDPIVVGVDVVEGSLRIGTPIAAVKKNPITGEMETISLGRVTSIELNHKAIPICKRGQPSVAVKIEGPNQPLYGRQLEEKDTLYSLISRVTIDTLKTHFREEVPKDDWQLIINLKRIFNVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5GL28</accession>
    <name>D5GL28_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 15">Dipeptidyl peptidase 3</fullName>
            <ecNumber evidence="4 15">3.4.14.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13 15">Dipeptidyl aminopeptidase III</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14 15">Dipeptidyl peptidase III</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">GSTUM_00009940001</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>Release of an N-terminal dipeptide from a peptide comprising four or more residues, with broad specificity. Also acts on dipeptidyl 2-naphthylamides.</text>
            <dbReference type="EC" id="3.4.14.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 17">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="15 17">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the peptidase M49 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.14.4" evidence="4 15"/>
    <dbReference type="EMBL" id="FN430344">
        <property type="protein sequence ID" value="CAZ85221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841030.1">
        <property type="nucleotide sequence ID" value="XM_002840984.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GL28"/>
    <dbReference type="FunCoup" id="D5GL28">
        <property type="interactions" value="607"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GL28"/>
    <dbReference type="GeneID" id="9185815"/>
    <dbReference type="KEGG" id="tml:GSTUM_00009940001"/>
    <dbReference type="eggNOG" id="KOG3675">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011977_1_0_1"/>
    <dbReference type="InParanoid" id="D5GL28"/>
    <dbReference type="OMA" id="QRYWIRD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008235">
        <property type="term" value="F:metalloexopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.540.30:FF:000001">
        <property type="entry name" value="Dipeptidyl peptidase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.540.30:FF:000002">
        <property type="entry name" value="Dipeptidyl peptidase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.30">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005317">
        <property type="entry name" value="Dipeptidyl-peptase3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039461">
        <property type="entry name" value="Peptidase_M49"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23422:SF11">
        <property type="entry name" value="DIPEPTIDYL PEPTIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23422">
        <property type="entry name" value="DIPEPTIDYL PEPTIDASE III-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03571">
        <property type="entry name" value="Peptidase_M49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007828">
        <property type="entry name" value="Dipeptidyl-peptidase_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 15" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="7 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 15 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 15" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="8 15" id="KW-0645">Protease</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 15 17" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="active site" evidence="16">
        <location>
            <position position="456"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="455"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="460"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="514"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR007828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR007828-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR007828-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAZ85221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="707" mass="78692" checksum="170359B3E8805B96" modified="2010-06-15" version="1">MSDEASIAPSPNKPPEPTSPPAALLADSPPTICRLEVAPHFAALTPKEKLYAHHISRASFLGTRVTLRQHSPESEAIYDLILALYAACAGDWKKLASEGPVSEKDVQYWLEYATVFLASLGNYKSFGDLKFVPRIPKAELRKLVEYADEEASKLFYGVEEVVYSFLPEERNLIGYIDAGHVSAYYPDSPSISKAEIEEVQAVTAANGILVENTRLKKVSDSEFHLLFASAEVTAPEGQKTEFVFGEGRKKLKLVYGDHAELMKKISAECRAAAERSANELQRKMWDEYARSFDIGSIQAHKESQKYWVQDKGPRVEANIGFVETYRDPAGVRAEWEGFAAMVNEGRTRTFGELVKRAEDFIARLPWGKDFEKDHFLKPDFTSLEVLTFAGAGIPAGINIPNYDDIRQEIGFKNVSLGNILSARSPNEVITFLKPEDVALYSELQGPAFEVQVGVHELLGHGTGKLLQETSPGEYNFDHANPPINPITNEPVGTWYGPNETWGSIFGGLAASYEECRAELVAMYLGVEKGLLSIFGHVTDQEAEDLLYIEYLQMARAGLLSVETWDPKSRKWGQAHSQARYFILQVFISAGDGFVTFSHSLPDMSDLTISIDRSKILTHGRKAVGEYLTKLHVYKSIADVGAGSELYKRMTEVTDQFKGYREVVMRLKQPRKVFVQANTVLSGDGGVELREYEDSVEGMVKSYAERGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GLQ9</accession>
    <name>D5GLQ9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00010360001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430351">
        <property type="protein sequence ID" value="CAZ85476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_002841239.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GLQ9"/>
    <dbReference type="STRING" id="656061.D5GLQ9"/>
    <dbReference type="GeneID" id="9186460"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010360001"/>
    <dbReference type="eggNOG" id="ENOG502S98B">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022048_5_2_1"/>
    <dbReference type="InParanoid" id="D5GLQ9"/>
    <dbReference type="OMA" id="TEHCIIC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="25"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ85476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="224" mass="25056" checksum="99ACDBFB5388792B" modified="2010-06-15" version="1">MGFSIRRGVTNLIRRGQRLAGNARCSVCMTYFKNALVKTIPACGHSYCKTCLQKTYTRAMNDPEGILPPRCCGQELTMAFAGDFLAGYIPKELEMPDSYGHTDFRRRYLEILAERTSDPIYCAHCKSLGKQVFIPPEEINEEEDSAVCSTCFQGTCTLCKRSNPHTTSNPCPVDSDESKQEIRYQRLSGRAGWRSCEGCGMMVSRDVGCAHVVCRCGQDVWLAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5GM41</accession>
    <name>D5GM41_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00010517001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430352">
        <property type="protein sequence ID" value="CAZ85584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841393.1">
        <property type="nucleotide sequence ID" value="XM_002841347.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GM41"/>
    <dbReference type="FunCoup" id="D5GM41">
        <property type="interactions" value="1064"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GM41"/>
    <dbReference type="GeneID" id="9187368"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010517001"/>
    <dbReference type="eggNOG" id="KOG3117">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065858_0_0_1"/>
    <dbReference type="InParanoid" id="D5GM41"/>
    <dbReference type="OMA" id="RHTKSER"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007146">
        <property type="entry name" value="Sas10/Utp3/C1D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13237:SF9">
        <property type="entry name" value="NEUROGUIDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13237">
        <property type="entry name" value="SOMETHING ABOUT SILENCING PROTEIN 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04000">
        <property type="entry name" value="Sas10_Utp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="191"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="267"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="196"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="270"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="324"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="380" mass="41525" checksum="C5BFEE9CD97F9AB5" modified="2010-06-15" version="1">MSPPPDLLETLTTLTASLTTTSTSIQSAEFPTLTSPENGISLLGLKNELLLSYVHNIVFLVLVRLRSGTLSNGIGADAVKELVKIRVLLERGVKPLEGKLKYQIDKVIAAASSSMPAGTAPATTKEEIEVRNGKGSGEDSSDEDEDVTANNCDYYPLQEKPRSDVAAASAPVSALTRKELAFRPNPAALVKPSAPALSSSTTNLSDGIYRPPRISATAMPEPNRSAEKGKPPRRKSHMLDEFVMEELSTAPTVESSVGSTIIDSGRVVKTARDRREERERQEYEENNFVRLPKMGKKEARELKRRGGREVNGMGGEDWSGFTGDIERLTKEVDRAGKSARLLEKSRKRKGDGDNEEGGSSRVVGDSFERKKERLSKRRKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5GM93</accession>
    <name>D5GM93_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00010593001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430353">
        <property type="protein sequence ID" value="CAZ85630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841439.1">
        <property type="nucleotide sequence ID" value="XM_002841393.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GM93"/>
    <dbReference type="GeneID" id="9187414"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010593001"/>
    <dbReference type="HOGENOM" id="CLU_2943510_0_0_1"/>
    <dbReference type="InParanoid" id="D5GM93"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ85630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="60" mass="6848" checksum="DBDE979EE3641B02" modified="2010-06-15" version="1">MAVWSIPNYYKIVRKVPGAWTISPAARAIIPPGNPIIRPKKISQVRPDRRKACESERQVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>D5GME1</accession>
    <name>D5GME1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00010668001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="FN430354">
        <property type="protein sequence ID" value="CAZ85684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841493.1">
        <property type="nucleotide sequence ID" value="XM_002841447.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5GME1">
        <property type="interactions" value="1245"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GME1"/>
    <dbReference type="GeneID" id="9183254"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010668001"/>
    <dbReference type="eggNOG" id="KOG0951">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000335_1_0_1"/>
    <dbReference type="InParanoid" id="D5GME1"/>
    <dbReference type="OMA" id="MNPKEFN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000004">
        <property type="entry name" value="RNA helicase, activating signal cointegrator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003287">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein 200 kDa helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000024">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.10:FF:000001">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000062">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.10">
        <property type="entry name" value="Sec63 N-terminal domain-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041094">
        <property type="entry name" value="Brr2_helicase_PWI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048863">
        <property type="entry name" value="BRR2_plug"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050474">
        <property type="entry name" value="Hel308_SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004179">
        <property type="entry name" value="Sec63-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057842">
        <property type="entry name" value="WH_MER3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961:SF4">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1 COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961">
        <property type="entry name" value="DNA POLYMERASE THETA, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05260)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21188">
        <property type="entry name" value="BRR2_plug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18149">
        <property type="entry name" value="Helicase_PWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02889">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23445">
        <property type="entry name" value="WHD_SNRNP200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00973">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158702">
        <property type="entry name" value="Sec63 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="487"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="674"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="1317"/>
            <end position="1430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="26"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="191"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="42"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="69"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="195"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ85684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1478" mass="167816" checksum="27DED5C001272D4A" modified="2010-06-15" version="1">MADQSQYKYANMSNLVLQVDKRFTHRRTDEATGDPESLAGKVDIKQMGDRALRDNAPKEKHIKKKKQLKEKDIDGLEDGKEEKRKRKRDAATSHGGSILSATDDVLGLVYHPRTAETREAYSLILTLVSTVLGDVSQSVVRSAADAILEILKDEDHSKKDFDKKREIDDILGEKMPNDKFTDLMNLSKKINDWNADGDESDEDESDESEDMEDKPKEEETESEDELVIGGARTRPGDRGKPTVDEYAVPAHDIDAFWLQRQISTKYPDPHLASSKTTIVFEILSDSEKTQRDVENDLMEVFDYDHFELVKLLMKNQPKIVWCTRLARAGESSEERVAIENEMVRDGCRRILDELRGREAEDGKEGKGKGKADLMEIDVPASAVPSASAAERKPGGLIGDLQPRGVVDLESFIFEQGSHLMTNASVKLPQGSTKRTFKGYEEIHVPAPKRKLDPNEPKLIPISDLPDWAKTPFVESKELNRIQTRCYPSAFEDDGNLLICAPTGSGKTNVAMLTILREIGKHRNPVTGNIKLDDFKIVYIAPLKALVQEQVGNFGKRLAPYGIKVSELTGDRQLTKQQIVETQIIVTTPEKWDVITRKATDLSYTNLVRTIRKMQTTQEPVRLIGLSATLPNYRDVANFLRVDMKTGLFHFDATFRPCPLRQEFIGVTDKKAIKQLQAMNDICYTKVIEQAANNQMLIFVHSRKETAKTAKYIRDKALELETIQRILKSDAGTREILQHEADQVADPNLKDLLPYGFAIHHAGMSRTDRSTVEDLFSDKAIQVLVCTATLAWGVNLPAHTVIIKGTQVYSPEKGDWVELSPQDVLQMLGRAGRPQYDTYGEGIIITKQAEMQYYLSLLNQQLPIESQFMRKLADNLNAEIVLGTVRTRYEAVEWLGYTYLYVRMLRSPALYSVGADYADDVALEQKRVDLIHSAAAVLEKCNLIKYDRKTGKLQSTELGRIASHYYISHNSMFTYNIHLQPALTTIELFRIFALSEEFKYIPVRPEERLELRKLLTRVPVPVKESIEEPQAKINVLLQAYISRLKLEGLALMADMVYVTQSAGRILRAIFEICLKRGWASVTKTALDLCKMAEKRMWPTMTPFRQFTAAPTNQLAPIVTKAERAAEFTWSKYFTMDPPHLGELFNSARAGKLAHSLLERFPRLDVQAQVQPVTRSLLKVELTITPDFIWDDAYHGTAETFWILVEDCDGEEILFHDQFILRKQYAESENNDHLVEFTVPMTEPMPPNYFITLVSDRWMHSETKLAVSFRKLILPEKFPPHTPLLGLVPLPVEALKKPEFVNLYPEWREFNKIQTQVFKSLFDTDDNMFIGAPTGSGKTVCAEFALLRHWSKDEESGRAVYIAPFQELVDQRHADWKARLSKLGGGKEIVKLTGETSADLKLLERGDLILATPTQWDVLSRQWQRRKNIQTVELFVRRSSTCGGTNGLHLRDGCIQNALHRCTDRKPNENRCSQRLIIKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMM9</accession>
    <name>D5GMM9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00010819001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430357">
        <property type="protein sequence ID" value="CAZ85772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841581.1">
        <property type="nucleotide sequence ID" value="XM_002841535.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMM9"/>
    <dbReference type="GeneID" id="9185108"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010819001"/>
    <dbReference type="eggNOG" id="ENOG502S712">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_162857_1_0_1"/>
    <dbReference type="InParanoid" id="D5GMM9"/>
    <dbReference type="OMA" id="SEGNHYC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ85772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="78" mass="8069" checksum="3F851AAC0F014FB9" modified="2010-06-15" version="1">MSSNNQGYSYKSSGTNSEGNHYCARDYGSGAANSNAYHYSNSDGSYYYSNANGSTYHSSSSGNSTYTAPSGGSGSGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMP8</accession>
    <name>D5GMP8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00010872001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430358">
        <property type="protein sequence ID" value="CAZ85791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMP8"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010872001"/>
    <dbReference type="HOGENOM" id="CLU_2039775_0_0_1"/>
    <dbReference type="InParanoid" id="D5GMP8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="85"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ85791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="121" mass="13088" checksum="DCA6106027A04491" modified="2010-06-15" version="1">MSIHKTSTSTLSTTYSSSSFTTHSPPRQCSTLDPLKPEATSRTPVYPRARPRQRASPCSRAGTTPARSCPQTQTAKPKKSLPKKILSGTGQVLGFCAWVLCFSCVLSGGRGFDEKHPHQPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5GMQ8</accession>
    <name>D5GMQ8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">1,3-beta-glucanosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00010889001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Splits internally a 1,3-beta-glucan molecule and transfers the newly generated reducing end (the donor) to the non-reducing end of another 1,3-beta-glucan molecule (the acceptor) forming a 1,3-beta linkage, resulting in the elongation of 1,3-beta-glucan chains in the cell wall.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Cell membrane</location>
            <topology evidence="1 5">Lipid-anchor</topology>
            <topology evidence="1 5">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the glycosyl hydrolase 72 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="5"/>
    <dbReference type="EMBL" id="FN430359">
        <property type="protein sequence ID" value="CAZ85801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841610.1">
        <property type="nucleotide sequence ID" value="XM_002841564.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GMQ8"/>
    <dbReference type="FunCoup" id="D5GMQ8">
        <property type="interactions" value="50"/>
    </dbReference>
    <dbReference type="GeneID" id="9185137"/>
    <dbReference type="KEGG" id="tml:GSTUM_00010889001"/>
    <dbReference type="eggNOG" id="ENOG502QRZZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021855_1_2_1"/>
    <dbReference type="InParanoid" id="D5GMQ8"/>
    <dbReference type="OMA" id="HDKCMTI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042124">
        <property type="term" value="F:1,3-beta-glucanosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071970">
        <property type="term" value="P:fungal-type cell wall (1-&gt;3)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004886">
        <property type="entry name" value="Glucanosyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468:SF4">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03198">
        <property type="entry name" value="Glyco_hydro_72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005126582" description="1,3-beta-glucanosyltransferase" evidence="5">
        <location>
            <begin position="19"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="452"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="361"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="418"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="425"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ85801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="472" mass="50893" checksum="A6A5B8D5FA56A36A" modified="2010-06-15" version="1">MKLQLSVLAAFLAGSASAVRPLIAKGGNFVNSAGDRFVIVGLAYQPGGASGYDPASGKDPLSDPDICLRDAAVMQRLGINTIRVYNVNPEVNHDMCMSIFNSVGIYVALDVNSPLAGDSIHRHEPESSYHEGYIKRIYSVVDAFRGYPNLLSFFAGNEVVNDALSASLVPPYIRAIHRDLKKYIAKHAPRTIPVGYSAADDPTRRPMWAYLSCGDDADSRTDFYGLNSYQWCGDSTWESSGYQGLVDTFKNTSVPLFFSEFGCNKIRPRPFGEIAALYGERMISSWSGGLVYEYSQEKSDYGIVQIDSNGDARLLKDFDNLQSQYNNINLNLIKTKSAATTTARHPKCEDTFILQGHSEKFNTSTTLPPNPATNLLEKGSGNTNVGKLVPVTQTKTPHKVFTSTGVQIDGIEIKMLKDDESNTPSGENTSSSTASSGSGGGSGDEKDATGGLRVSAVLVVGVAVVVGFWGLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>D5GN96</accession>
    <name>D5GN96_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="13">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00011168001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the ABC transporter superfamily. ABCB family. Heavy Metal importer (TC 3.A.1.210) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430362">
        <property type="protein sequence ID" value="CAZ85989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841798.1">
        <property type="nucleotide sequence ID" value="XM_002841752.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GN96"/>
    <dbReference type="STRING" id="656061.D5GN96"/>
    <dbReference type="GeneID" id="9187352"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011168001"/>
    <dbReference type="eggNOG" id="KOG0056">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_6_1_1"/>
    <dbReference type="InParanoid" id="D5GN96"/>
    <dbReference type="OMA" id="SWWEYLK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044604">
        <property type="term" value="F:ABC-type phytochelatin transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036249">
        <property type="term" value="P:cadmium ion import into vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098849">
        <property type="term" value="P:cellular detoxification of cadmium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071996">
        <property type="term" value="P:glutathione transmembrane import into vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036246">
        <property type="term" value="P:phytochelatin 2 import into vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18583">
        <property type="entry name" value="ABC_6TM_HMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03253">
        <property type="entry name" value="ABCC_ATM1_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000186">
        <property type="entry name" value="ATP-binding cassette sub-family B member 7, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000050">
        <property type="entry name" value="Vacuolar ABC heavy metal transporter (Hmt1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221:SF651">
        <property type="entry name" value="HEAVY METAL TOLERANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="117"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="182"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="238"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="345"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="386"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="466"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="491"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="12">
        <location>
            <begin position="348"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="669"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="52"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="262"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="909"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="977"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1078"/>
            <end position="1107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="270"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="909"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="921"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="977"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="1024"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1036"/>
            <end position="1058"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1081"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1094"/>
            <end position="1107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ85989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1107" mass="121608" checksum="FBBAF2BC499E9BA2" modified="2010-06-15" version="1">MENNGNIVSLGDTYHPGTSLAPDVVFYIHYAYPVSLFFFFLFTLTWWGISTSESSKPTPPPVTGIYDNGKRKKRRQRSGRANSGLFGRIAERFPGGAKAKGKKVADDKLTPLRKAAFNWLLVGVIFTLVANSTNVILHALTKKGWWCGKDYVICTVSLLFIYAIILISQLDNILYPTLPHLASWFFGLVGEVVLLVANIFTYGEKHGPLIGGSGVGVGASKNRFSLHNWDIVDLSIDVLRIVFLVLLVGLYVFLIILSGQESDDSDSEESSSLLASSSSSTVDGAPDYGSVPAHRKHGVPEPSTHAPAGWGRRAVVGKQSWWEYVQGYTIFFPYLWPSKDRRLQFLMIICFILMVLQRGINVMVPIQLGKITNILGGSNGELPRLPYVQILLYIVYRFLQGNMGILGAIRSMLWIPIGQYSYQALSTSAFEHVHSLSLDFHLGKKTGEVLSALSKGNAINTFLEQVTFQVVPMLFDLVVAVVYFLVSFDAYYALIVAIVTLCYLYITVRMAQWRADIRREMVNRSRDEDAVKNDSMVAYETVKYFNAEGYEFGRYRDAVRAYQKAEYKVLFSLNIMNVTQNLVFTIGLVVACFLSAWQVSTGETSVGSFVSLLAYLAQLQGPLNFFGTFYRSIQSSMINSERMLELFKEQPTVVDAPHAEKLTKCEGEISFKDVHFSYDARKPALRGLDFVAKPGTTTAFVGESGGGKTTVLRLLFRFYNIEEGSIEVDGKDVRDITIDSLRSHIGVVPQDTVLFNESVMYNLKYANPNATDEMVFEACRAASIHDKIMSFPDRYNTKVGERGLRLSGGEKQRVAIARTILKNPKIILLDEATAALDSETEQHIQAALRELAKGRTTLVIAHRLSTITAANQILCLHEGQVAEAGTHDELLAKNGRYAMMWRKQIRAEQKQKKAAMKKDDESESSTEDTDESLIDLAPSHPSGFPGNGSMLENEAVTPEMIDTPPGELSLMPVIASAQNTAGGGPSTTEIEVTPKTAAAERVVEESPVLSPVTSPLASPEPPSRKGKGKNRGGLRRSGSALSASVGGSSGGPPLSSHGKWSTRGIKRALSFKDSILHRRSAGVAGSGSPSGSERGSERGSGRSIDNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNC1</accession>
    <name>D5GNC1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00011217001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RRN3 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430363">
        <property type="protein sequence ID" value="CAZ86014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841823.1">
        <property type="nucleotide sequence ID" value="XM_002841777.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNC1"/>
    <dbReference type="FunCoup" id="D5GNC1">
        <property type="interactions" value="616"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GNC1"/>
    <dbReference type="GeneID" id="9182214"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011217001"/>
    <dbReference type="eggNOG" id="KOG2434">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010579_1_1_1"/>
    <dbReference type="InParanoid" id="D5GNC1"/>
    <dbReference type="OMA" id="VCSPAIV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001042">
        <property type="term" value="F:RNA polymerase I core binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001181">
        <property type="term" value="F:RNA polymerase I general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006361">
        <property type="term" value="P:transcription initiation at RNA polymerase I promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007991">
        <property type="entry name" value="RNA_pol_I_trans_ini_fac_RRN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12790:SF0">
        <property type="entry name" value="RNA POLYMERASE I-SPECIFIC TRANSCRIPTION INITIATION FACTOR RRN3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12790">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IA RRN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05327">
        <property type="entry name" value="RRN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="27"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="581"/>
            <end position="618"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ86014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="618" mass="69929" checksum="04FA42C899936BA6" modified="2010-06-15" version="1">MPAPLRGTLFYRFGSKLPAMGGSEARSVIGVKREQPISESDSENKGPSSKRIKKNAGMKAKATEINATNSLFRKFVNNALDERAAGSNANFELLRRKFSADPGNEDAPSSLELQNTILALTNTVSRLNGSCSQLVEDIINCQWVARESGFVVSYVKLLGNLVSAHSNYMDLIVNMLVQHLVWLRSSAGQLPNHTPVKRSVIYERVHYALQFILDLVPTAAFSTLFPALISEFPHKSESKKAHVTYVTNLLRVIEYAPSLRNKLLTFITDRVIKIDTEIQADLDDLEDEQGEELEAEITETEMERNGDMVTNIKDTVDKLDVMLDLLFSYYSKSFPNKSVESPTQESIENFTLIMRLFNSIILPTYRSRYTQFLVFWAAQKAPRFSDTFCAAAITNALDNALQPGTRQAAAAYVASFIARAKLMPGQYVQMATNVMCRWLGTFVDERSAECEGPDVSKWGGFYSVCQAVMYIFCFRWKDLRVVEEDEFGIIGERWMGFLQDLNKAIISKFNPLKVCSPAVVETFAKIASYFQLVYCFTIIEQNKRKGLSSQEELDSYFPFDPYNLRRSKQWIQGCYNEWEPIEGLENEEEEDNDSSGESDNDNESIVEEEVDDESEAHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNK4</accession>
    <name>D5GNK4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">chitinase</fullName>
            <ecNumber evidence="2">3.2.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00011326001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.14"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the glycosyl hydrolase 18 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.14" evidence="2"/>
    <dbReference type="EMBL" id="FN430365">
        <property type="protein sequence ID" value="CAZ86097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841906.1">
        <property type="nucleotide sequence ID" value="XM_002841860.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNK4"/>
    <dbReference type="FunCoup" id="D5GNK4">
        <property type="interactions" value="143"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GNK4"/>
    <dbReference type="GeneID" id="9186173"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011326001"/>
    <dbReference type="eggNOG" id="KOG4701">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007818_2_1_1"/>
    <dbReference type="InParanoid" id="D5GNK4"/>
    <dbReference type="OMA" id="NSVQTWS"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008843">
        <property type="term" value="F:endochitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001579">
        <property type="entry name" value="Glyco_hydro_18_chit_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050542">
        <property type="entry name" value="Glycosyl_Hydrlase18_Chitinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45708">
        <property type="entry name" value="ENDOCHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45708:SF49">
        <property type="entry name" value="ENDOCHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01095">
        <property type="entry name" value="GH18_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0146">Chitin degradation</keyword>
    <keyword evidence="6 8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072172" description="chitinase" evidence="10">
        <location>
            <begin position="23"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="GH18" evidence="11">
        <location>
            <begin position="40"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ86097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="325" mass="34961" checksum="5A78E795733E55B6" modified="2010-06-15" version="1">MFSSMLFSTFCLASALLSIVHAAALPVCGARQEPAISKAGKTVAYFGQSQSQSTDSLAKFCADTSVDIIVLAFVNVIKGRRGLPGLNLSGYCWDPIDGTDLHRCPEFGTAIKDCQARGKLVLMSLQGASGMQTLADDAAACEYADNLWRLFGEGTGLESMRPFGDARVDGFDIDNENNNSNGWPKFISELRNKFASASKKYYISAAPQCVRPDKSIGSAIYLVDYLFIQFYNNYCATTGLVSCFNNWSEDIAANGIAGAKVFAGFLGAPDKGQGYAAHSEMIGYVSQIRSKSNFGGVSVWDATCANNNVDADGRTFLKAMRDSLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNQ3</accession>
    <name>D5GNQ3_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mitochondrial import inner membrane translocase subunit TIM54</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00011426001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TIM54 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430367">
        <property type="protein sequence ID" value="CAZ86150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002841959.1">
        <property type="nucleotide sequence ID" value="XM_002841913.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNQ3"/>
    <dbReference type="STRING" id="656061.D5GNQ3"/>
    <dbReference type="GeneID" id="9182785"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011426001"/>
    <dbReference type="eggNOG" id="ENOG502QPMQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039097_0_0_1"/>
    <dbReference type="InParanoid" id="D5GNQ3"/>
    <dbReference type="OMA" id="KRNIAKW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021056">
        <property type="entry name" value="Mt_import_IM_translocase_Tim54"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11711">
        <property type="entry name" value="Tim54"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="142"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="142"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="156"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ86150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="345" mass="38044" checksum="A08AE49A9F4D18ED" modified="2010-06-15" version="1">METQPPPPAAPPPPPPQGNPAFRAMGLPNIRAKFPSRNWSIFISLSTAFAAAVYYDRRETKRVQKHWCSVVEHLAREPLSTLELPRKVTVYLSAPPGDTITPSREYFREYVKPVFTAAALEFDVGLEGGMVLFGLEFKDPNATPPPQEFKPPPTSKLDRALAETPDPDKKAREEENKKKASIPSPHILPHAYNTSPTPSSLPETFDPVGFIPHAHILGFLNTPIRLYRFLNHRKTADLAGREAAAIALGIYSRPFNLALEPSSTMMTNEDVKGGLGAGREGGEIGRACGREEEAEWPKRVWKEEGTGTGEITEEVVVDHRIGGRMRRFVLPEGAEAVVDAEKGKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>D5GNX3</accession>
    <name>D5GNX3_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">arginine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Arginyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00011569001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>tRNA(Arg) + L-arginine + ATP = L-arginyl-tRNA(Arg) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:20301"/>
            <dbReference type="Rhea" id="RHEA-COMP:9658"/>
            <dbReference type="Rhea" id="RHEA-COMP:9673"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78513"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.19"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.19" evidence="2"/>
    <dbReference type="EMBL" id="FN430371">
        <property type="protein sequence ID" value="CAZ86216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842025.1">
        <property type="nucleotide sequence ID" value="XM_002841979.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GNX3"/>
    <dbReference type="FunCoup" id="D5GNX3">
        <property type="interactions" value="956"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GNX3"/>
    <dbReference type="GeneID" id="9183509"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011569001"/>
    <dbReference type="eggNOG" id="KOG1195">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006406_6_2_1"/>
    <dbReference type="InParanoid" id="D5GNX3"/>
    <dbReference type="OMA" id="YEFKWER"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004814">
        <property type="term" value="F:arginine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006420">
        <property type="term" value="P:arginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07956">
        <property type="entry name" value="Anticodon_Ia_Arg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00671">
        <property type="entry name" value="ArgRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000006">
        <property type="entry name" value="Arginyl-tRNA synthetase 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000058">
        <property type="entry name" value="Mitochondrial arginyl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.70">
        <property type="entry name" value="Arginyl tRNA synthetase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00123">
        <property type="entry name" value="Arg_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001278">
        <property type="entry name" value="Arg-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005148">
        <property type="entry name" value="Arg-tRNA-synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036695">
        <property type="entry name" value="Arg-tRNA-synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035684">
        <property type="entry name" value="ArgRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008909">
        <property type="entry name" value="DALR_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00456">
        <property type="entry name" value="argS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956:SF11">
        <property type="entry name" value="ARGININE--TRNA LIGASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956">
        <property type="entry name" value="ARGINYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03485">
        <property type="entry name" value="Arg_tRNA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05746">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00750">
        <property type="entry name" value="tRNA-synt_1d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01038">
        <property type="entry name" value="TRNASYNTHARG"/>
    </dbReference>
    <dbReference type="SMART" id="SM01016">
        <property type="entry name" value="Arg_tRNA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00836">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55190">
        <property type="entry name" value="Arginyl-tRNA synthetase (ArgRS), N-terminal 'additional' domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arginyl tRNA synthetase N-terminal" evidence="12">
        <location>
            <begin position="49"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="DALR anticodon binding" evidence="11">
        <location>
            <begin position="516"/>
            <end position="633"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ86216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="633" mass="71472" checksum="421EDF410880C812" modified="2010-06-15" version="1">MATESTTGPITGVPPSPSLESLNKQLNNCGISEPLTPFPGSNPTTNPVDVYRCYIAHVLAPITGVELELVYPALEWTQNLEKGDLILAAPRLRIPGKNPSELTKEWAEKFPENPYVRSPTAAGTFLQFYFKKDVLSKLVLSSILSRKSAYGPIDTGKGKRVIVEFSSPNIAKPFHAGHLRSTIIGGFLSNLYESCGWDVIRMNYLGDWGKQFGILGVGYKRFGSEEKLLEDPINHLFEVYVAINKVVEQEKEAEKNKAAEKGEKQISNGPTDQEALEFFFKMEHGSEECIAMWKRFRELSIEKYKATYSRLNISYDVYSGESQVSKESMERAGEMMREMGISSESDGAIVVDFKPHAKKLGKAVVQKKDGTTLYLTRDIGAAMERYEKYKFDKMIYVVACQQDLHLQQLFMILKLLKLDWADRLTHVNFGMVLGMSSRRGTVVFLDDILADARDKMHEVMRKNEEKYKQVEDPEMVADVVGRTAVMIQDMSGKRINNYTFDMARMTSFEGDTGPYLQYAHSRLCSIQRKSGIPIPELLNADFSLLTEDHAINLIRCLAQYPDVLQNTLKTLEPTTVVTYLFKMTHLLSSSYDVLRVIGEEPRLSAARMALYESARQVLNNGMRLLGLTPVERM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>D5GPN2</accession>
    <name>D5GPN2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00011944001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHEK2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430377">
        <property type="protein sequence ID" value="CAZ86475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842284.1">
        <property type="nucleotide sequence ID" value="XM_002842238.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GPN2"/>
    <dbReference type="FunCoup" id="D5GPN2">
        <property type="interactions" value="425"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GPN2"/>
    <dbReference type="GeneID" id="9186715"/>
    <dbReference type="KEGG" id="tml:GSTUM_00011944001"/>
    <dbReference type="eggNOG" id="KOG0615">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_171_0_1"/>
    <dbReference type="InParanoid" id="D5GPN2"/>
    <dbReference type="OMA" id="IGRHVEC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22670">
        <property type="entry name" value="FHA_MEK1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001380">
        <property type="entry name" value="Checkpoint kinase 2-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHA" evidence="6">
        <location>
            <begin position="151"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="242"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="116"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="561"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="54"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="116"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="137"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="561"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="577"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="271"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ86475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="621" mass="69074" checksum="E6009F3AA3894862" modified="2010-06-15" version="1">MTPRVEGAAVRKRSQPSSPASNKNSPKKVKRQPDDDVDQVDGSAIVEDRESLESLLNPQGDVTITRTPASSPPPQDTQVASQFYYPPLSEQGVDEDDETIWGYLWPLTQKNQSVIKLNKTPQVSTPNKDSPKSAEKKKPKNNNKKHGTGGFVIGRHVECDLVIDHNVISNRHCVIYKENAGSHPVAVLEDLSSNGTWVAGVIIGRNRSRTLENGDEIEFPGGFQYTFRYPPHMQSSAFRNAYDLGQQLGSGHFATVHLAVDKKSGQEFAVKIFRKRRNEDKSKTSGLQQEIAVLMSVNHPNILCLQGTYDEDDGVYLVLELAREGELFNYVIRNTKLSEVDTRKVFVQLFNGLKYLHERNIVHRDIKPENILLCDTDLTVKLADFGLAKIIGEDSFTTSLCGTPSYVAPEILEPSRNRKYTRAVDMWSLGVVLYICLCGFPPFSDELYTPDNPYNLSQQIREAKYDFPSPYWDDIADPALYLIESMLEVNPNKRITVDMALKHAWTVEGTFNPAESCDSLVGALETMGLRRSVGRERTLLAEHPEIRPTRAVETAVVMSPVTSPSVVPESEGGGEEVVTRETEVREGNPSDIPAVMTAFMHVGGRGGDETLYPDDSFAESA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>D5GQ11</accession>
    <name>D5GQ11_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Guanine nucleotide-exchange factor SEC12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00012127001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Guanine nucleotide-exchange factor (GEF) required for the formation or budding of transport vesicles from the ER.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Endoplasmic reticulum membrane</location>
            <topology evidence="10">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Golgi apparatus membrane</location>
            <topology evidence="10">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the WD repeat SEC12 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430380">
        <property type="protein sequence ID" value="CAZ86604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842413.1">
        <property type="nucleotide sequence ID" value="XM_002842367.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GQ11"/>
    <dbReference type="FunCoup" id="D5GQ11">
        <property type="interactions" value="153"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GQ11"/>
    <dbReference type="GeneID" id="9185182"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012127001"/>
    <dbReference type="eggNOG" id="KOG0771">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_523761_0_0_1"/>
    <dbReference type="InParanoid" id="D5GQ11"/>
    <dbReference type="OMA" id="EPQLAIF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003400">
        <property type="term" value="P:regulation of COPII vesicle coating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045260">
        <property type="entry name" value="Sec12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23284">
        <property type="entry name" value="PROLACTIN REGULATORY ELEMENT BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23284:SF0">
        <property type="entry name" value="PROLACTIN REGULATORY ELEMENT-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 10" id="KW-0813">Transport</keyword>
    <keyword evidence="2 10" id="KW-0853">WD repeat</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="416"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU369019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ86604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="498" mass="53283" checksum="CCA30BF5D04B9513" modified="2010-06-15" version="1">MRWLAKNEAQISGTRRQLLFPPRVGLVFFLPQKAPLPNDLTYNMAPSITSEKLTLSYPLFGASFIDADRLLVAGGGGEGRSGVGNKITVLDTLTKPGKPLTVTSEIELSKDEDAVMSLSALSTSSGVKALAGINSSQKAKSNEHFRVFDVPKEGEISLASKHALFSHPDAETYQRLVRVDGEGKAAVIASGSGLAPASASEVVIIDVSSLSVKRRIRLPGKEEVGDVDISRDGKRVAYCSPGDIYIASTEKEEQRLEPIALKWKSSDRPKGSFRSVRFSNNGGLAVVYNLFGRSGAIILLIDPSGEVISRRNVHCGVKAVTSVDSLMLSPTSTLLAVAGADRSVQLFLVGGQIKAVKTFKDVHPFQITKLAFSPLPSSGTTVKLATTSMGNTVVVFYIPVFETEGKWQLRQTSVKLTAFLVLISLVGVVVFAIALQLMFNARAGFETEQERTQELERPGKSGLEAAEAAGEWVLGEGMEVDVGGMEDTLEEAIQGVVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>D5GQ33</accession>
    <name>D5GQ33_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00012180001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430382">
        <property type="protein sequence ID" value="CAZ86626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842435.1">
        <property type="nucleotide sequence ID" value="XM_002842389.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GQ33"/>
    <dbReference type="STRING" id="656061.D5GQ33"/>
    <dbReference type="GeneID" id="9185206"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012180001"/>
    <dbReference type="eggNOG" id="KOG0235">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033323_13_0_1"/>
    <dbReference type="InParanoid" id="D5GQ33"/>
    <dbReference type="OMA" id="RCFIVRH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050278">
        <property type="term" value="F:sedoheptulose-bisphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046390">
        <property type="term" value="P:ribose phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050275">
        <property type="entry name" value="PGM_Phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48100">
        <property type="entry name" value="BROAD-SPECIFICITY PHOSPHATASE YOR283W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48100:SF15">
        <property type="entry name" value="SEDOHEPTULOSE 1,7-BISPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ86626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="200" mass="22210" checksum="F68686EB57260879" modified="2010-06-15" version="1">MRIKATGEALIGEGKLICPSLLAYVYVSPRLRAKRTLELLGLFEKCPHLCGQSTETDQLVEWDYGDYEGLTSPEIRERRRINGVLGDEPWDIWRDGCVGGESPEDVERRCDALIKTIREQWHSPAMRDGNIRGDVVCVAHGHLLRAFAMRWVGKSISSNTVLLILEAGGVGVLSYEHHSIDEPAILLGGSFVVKAESPKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>D5GQ46</accession>
    <name>D5GQ46_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00012195001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430382">
        <property type="protein sequence ID" value="CAZ86639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842448.1">
        <property type="nucleotide sequence ID" value="XM_002842402.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GQ46"/>
    <dbReference type="STRING" id="656061.D5GQ46"/>
    <dbReference type="GeneID" id="9185219"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012195001"/>
    <dbReference type="eggNOG" id="KOG0158">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_14_7_1"/>
    <dbReference type="InParanoid" id="D5GQ46"/>
    <dbReference type="OMA" id="PRICPRE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050121">
        <property type="entry name" value="Cytochrome_P450_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305:SF166">
        <property type="entry name" value="CYTOCHROME P450 12A4, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="3 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602403-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ86639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="262" mass="29173" checksum="2075A4B441DFA058" modified="2010-06-15" version="1">MTEALATNLLENGNTTGKRNMSHDLLQCRNDDNTPLSKKELVGEMLAFTTAGSDPTAYEISAILDRICRHGDVREKLLQELRAVGELEQSSAEGVVTYAQTLRLPYLLAVVKETMRLNPAFQGQFSRVAPEGGCGGEVGGLEVLPGVVVPGGVWLSINTYVSQRDKLVFGEDAEEFKPERWLPIGGDRYHAMSKYLSVFGYGSTACMGQHLASQKINKTVVEILRRFNVELKDPKTPLKERNIIQMFISDLFMTFTEREKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5GQ81</accession>
    <name>D5GQ81_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00012250001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430383">
        <property type="protein sequence ID" value="CAZ86674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842483.1">
        <property type="nucleotide sequence ID" value="XM_002842437.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GQ81"/>
    <dbReference type="STRING" id="656061.D5GQ81"/>
    <dbReference type="GeneID" id="9184031"/>
    <dbReference type="KEGG" id="tml:GSTUM_00012250001"/>
    <dbReference type="eggNOG" id="KOG0137">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0537">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_4_4_1"/>
    <dbReference type="InParanoid" id="D5GQ81"/>
    <dbReference type="OMA" id="EQPVMRQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050741">
        <property type="entry name" value="Acyl-CoA_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48083:SF28">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_6G10880)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48083">
        <property type="entry name" value="MEDIUM-CHAIN SPECIFIC ACYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00363">
        <property type="entry name" value="CYTOCHROMEB5"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="6">
        <location>
            <begin position="3"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ86674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="511" mass="57041" checksum="4ABB3680468E7B33" modified="2010-06-15" version="1">MATQTFTREQVSKNNNADSLHVIIDSKVYDLTDFTDAHPGGVHALLQVAGQDATSEFYSMHRHEILTKYANLVIGTIQGESPQVIEPKAGDLCPIPYTEPMWLSPDFKSPYYDETHRRLQKEVRVFVDEYVRPEALRIEEAGERPSAEIIKRMAENGINAMRLGPGRHLEGYNLIGGIEPKEFNYFHELVLNQELVRTHGRACNDGFLGGMVIGLPPVLNFCRNTALRDRVMRECFSGEKSICLAVSEAFAGSDVASIRTTAVKSADGTHYVVNGTKKWITNGNWADYFTTAVKTEKGYSMLLIERGEGVETEQIKTSYSSAAGTAFITFDNVNVPVENLLGKEHQGFPVIMSNFNHERWVMACASIRASRVVVEECLKWANQRKVFGRRLVDQPVIRGKLAKMISLVESGQSWLEAITYQMCNMTYQQQAKSLAGPIALLKNFITRAAHEIADESVQVFGGRGITKSGMGRVIENFHRTYKFDAVLGGSEEILGDLAVRQALKNFPKSML</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>D5G416</accession>
    <name>D5G416_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00003905001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835138.1">
        <property type="nucleotide sequence ID" value="XM_002835092.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G416"/>
    <dbReference type="STRING" id="656061.D5G416"/>
    <dbReference type="GeneID" id="9182386"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003905001"/>
    <dbReference type="eggNOG" id="ENOG502SCE7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1679240_0_0_1"/>
    <dbReference type="InParanoid" id="D5G416"/>
    <dbReference type="OMA" id="PEEFLIQ"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ79259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="157" mass="18194" checksum="EBC46D93E920C769" modified="2010-06-15" version="1">MLPSPPSSPLEFLAPKRLFKPIPTSHSREEGREARRNLFLKKVRDARDEKLMKVRGGEDEMMRLIFVSEKKRWEASQARTAASIPTIYEEEEFGIGNEELDFQAEESLFMDQDEPGPSEDLDEMLDRDGKELEDLLSQLDLQLHQVMPEANREMMPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4A4</accession>
    <name>D5G4A4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00004020001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429986">
        <property type="protein sequence ID" value="CAZ79347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835226.1">
        <property type="nucleotide sequence ID" value="XM_002835180.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4A4"/>
    <dbReference type="STRING" id="656061.D5G4A4"/>
    <dbReference type="GeneID" id="9184972"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004020001"/>
    <dbReference type="eggNOG" id="KOG1208">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_44_6_1"/>
    <dbReference type="InParanoid" id="D5G4A4"/>
    <dbReference type="OMA" id="CKVYLAC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320:SF148">
        <property type="entry name" value="NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320">
        <property type="entry name" value="RETINOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ79347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="338" mass="35993" checksum="73DB4F2D19CF568E" modified="2010-06-15" version="1">MPIQLKESDLPSLPSKTALVTGATSGIGLQTAILLASHGATVHIASRNPAKGEETVTGLRAQYPSHTYHHHPLDLSSAASAAASAAHFARENARLDILVANAGVAFVPVDELSADGLDMAFAVNHVGHFVFVRGLLPLLRRTAEGSGDVRVVVTSSAAYSFVDKVDLSDVACRVPGDGGSVRHLPAAGRRYGRSKRANILFAMELDRRLRAPDGAGAGVRVNVCHPGTIGGTALGDNGAFGVPAWGSRLLHGLISTVSLTPREGAMTQTLLAAADRVREEDVHGCFFAPRTGWNMRYTHSEEVDLGEWARNEEDAKVLWEWTEEWVDKVLGKGNWGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>D5G4H7</accession>
    <name>D5G4H7_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Peroxisomal ATPase PEX1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Peroxin-1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">GSTUM_00004111001</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN429987">
        <property type="protein sequence ID" value="CAZ79420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842500.1">
        <property type="nucleotide sequence ID" value="XM_002842454.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G4H7"/>
    <dbReference type="FunCoup" id="D5G4H7">
        <property type="interactions" value="191"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G4H7"/>
    <dbReference type="GeneID" id="9184049"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004111001"/>
    <dbReference type="eggNOG" id="KOG0735">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_1_0_1"/>
    <dbReference type="InParanoid" id="D5G4H7"/>
    <dbReference type="OMA" id="LSASWCA"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016558">
        <property type="term" value="P:protein import into peroxisome matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19526">
        <property type="entry name" value="RecA-like_PEX1_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000149">
        <property type="entry name" value="Nuclear valosin-containing protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029067">
        <property type="entry name" value="CDC48_domain_2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015342">
        <property type="entry name" value="PEX1-N_C-lobe"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF12">
        <property type="entry name" value="PEROXISOMAL ATPASE PEX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09262">
        <property type="entry name" value="PEX-1N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54585">
        <property type="entry name" value="Cdc48 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="14">
        <location>
            <begin position="531"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="14">
        <location>
            <begin position="816"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="202"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="281"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1079"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1140"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="209"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="295"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1081"/>
            <end position="1098"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAZ79420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1165" mass="124729" checksum="EFB600BDE54E4759" modified="2010-06-15" version="1">MSRKPSPPATPAIVSLTPLKNCLLNLPVALVNVLVSANTPAQNVIVELSFRASVGGGSTAATTRSVYAGWTGMQSKRKPGAGSGRSEEAVVEMDPAFARNVGLSEGSKVSIHLHLDPTPAHTFHIEPLTASDWEIIELHADFLELNLISQIRCVSPAHPISVYLSPTSTASIRVTKVEPEDALSSPNGFAKVSPEAEIIVAPKKRQRRQSQGAKSATSTVKTSMKKRSDSGGAGAMFFRGVSEEVAAGGKGFSVYLDPTLLMSSRHLRSVSHVVVSVVRPPAIAPPQDPSQQQQQEEKDKKDKKDATPEVVPASKIVAVVRPWDSAPDGKHVGLSKLLSTALYLRPGQVGDVIRLEPAPQQVPQKLLSKVKVFPFHGTEKQDGLKWGGGTKRLEEMAIARLQEVLGPHRQRNGTKAMIEEDKTSVSPGESVLEGALTDGLILPAIRDSPIAAGGVLKIEVSAGEGGEAPQYIKWVLVADKKPPLEIGSLVPKPTPPPAVVSETLFVPGTDSDQPIVGVDDILSSVTDHLLSSSSVLLSGAHGSGKTSILRLIAKRLSGAPHFHRIIDPGSLSKLSEERVGTVKENVSRWFTEAIWAAGKTGHSILLLDDLDRLCASESENQDNSRVRQIAEVVVAAVRKYTSPGGAGGRGGVIILASCQAKESMHSLVVSGHVFREALSLKAMSKEGRRAVLDGVVGATSGVLEKGLELRDIASKTEGYMPGDLVLLVGRARHEAVVRMVDAGESKESPILTLRRIDFDSALRGFVPAGLRGVKLQTSGASWKDIGGLTETRRILLETLEWPTKYAPIFASCPLRLRSGLLLYGYPGCGKTLLASAVAGECGLNFISVKGPEILNKYIGASEKSVRDLFERASGAKPCVLFFDEFDSIAPKRGHDSTGVTDRVVNQMLTQMDGAEGLDGVYVLAATSRPDLIDPALLRPGRLDKSLLCDLPNLEDRLDIFRALVGEGGKLRVDPDVSLEELAARTEGYSGADLQAVLYNAHLEAVQGIISEELRKREALEEEQEGAGAAGKDSKEELDFLEFVWGDSRKGKGRAPPVVSASRHRERIALLEKLESLKKKSSNNSTFSNSNNNSVSVTKTKQDAGTAEVTISWSHVESSLYSTRPSISPQERKRLQGIYTEFVGQRNGEMPSGQGSTEVGGRSSLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5G571</accession>
    <name>D5G571_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00000282001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN429996">
        <property type="protein sequence ID" value="CAZ79656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835499.1">
        <property type="nucleotide sequence ID" value="XM_002835453.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G571"/>
    <dbReference type="STRING" id="656061.D5G571"/>
    <dbReference type="GeneID" id="9186354"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000282001"/>
    <dbReference type="eggNOG" id="KOG0051">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_419315_0_0_1"/>
    <dbReference type="InParanoid" id="D5G571"/>
    <dbReference type="OMA" id="DNSVWIP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051651">
        <property type="entry name" value="DMTF1_DNA-bind_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46380">
        <property type="entry name" value="CYCLIN-D-BINDING MYB-LIKE TRANSCRIPTION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46380:SF2">
        <property type="entry name" value="CYCLIN-D-BINDING MYB-LIKE TRANSCRIPTION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH myb-type" evidence="6">
        <location>
            <begin position="287"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="5">
        <location>
            <begin position="287"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="5">
        <location>
            <begin position="334"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="6">
        <location>
            <begin position="387"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="58"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="128"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="523"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="631"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="85"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="129"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="177"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="192"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ79656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="654" mass="72340" checksum="B4153C859222E68E" modified="2010-06-15" version="1">MTVAGGGDDLPDSQRIISQHLAAHMSKITPASASAGAALHEDDDDLLMMETELGYTAVSSPPPLDADDVLSSSAFHTSPRKKGRAVYEKKKNKRTDPIEDANRLVDPALMEFDEATAAAAAAVSIPPDMLRDGAEEPKKRKRRLPTGKLDDGPEKKKKSHKKKASEGIVNSEAASLVQPSSSAPLQQLPIQATPSTPGPISTTATATPSGGSAGGTFSVEEKQAIDVHLARYAEDNGLAHADLCRRVWANERRKDDFWEGVCSVLPQRSRASIYKHVRRQYHVFSSRAKWTESEDEHLATLVKEKGSAWKAVGDAMGRMGEDCRDRWRNYGKCGKDRGKDRWDESEESELKKVITKVLTGIRTRRGITDPFDLEANTIDYDGESEINWTVISDLMDNKRSRIQCRYKWNKMKQQKARSGGVAVVKSPGTWKKKAKAFVEDMLVGDHIWLLKQIRDSGATTEKTIPWENIALNDKIWSAKELERAYKKLRQTVPHQRLPLSEIVNKVLKDLEDLSEDVKGQRYLPEVNGTPAAADGPQSMAEDQLSQSSATPHIPDQFDASNYTYEQQPMIQNVAPVASMSIADGEYDPNRAAEDMALVDPELIGQGIEHGVQQDLEKTAGEAIMIAAAAAARERERSGEDETERELRRRLGVEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5F4</accession>
    <name>D5G5F4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00004310001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835590.1">
        <property type="nucleotide sequence ID" value="XM_002835544.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5F4"/>
    <dbReference type="GeneID" id="9183733"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004310001"/>
    <dbReference type="eggNOG" id="ENOG502SE9H">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007974_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5F4"/>
    <dbReference type="OMA" id="WANLESM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027974">
        <property type="entry name" value="DUF4470"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14737">
        <property type="entry name" value="DUF4470"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4470" evidence="1">
        <location>
            <begin position="16"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="875" mass="98091" checksum="E2E93246C7F5BDBA" modified="2010-06-15" version="1">MSHKTIYWPEPFFYAIGNTVASCLTQDLPPEEPAKVLSLGCGDPRSILFSVQCAKMDFDYDFTCCDIESGILARNVILLALIFEGNKSEARSEKLWNIFYHIKLDRESLSALSDQCEKLVNVSADISSWNNSRYASFMRMCDKRTLSKLRAQWQTYIKNAKLVGREKKEYGEKFNKALAGTLLKVPKHDISSTLASGPLTMEAVESTQSLLRHYWVKGTYSMDPKVIATTTIPNPTLSHSFSGKVLALHYGSDPLLGIHIGPAFCGLKSEPKVYKKKVEPSHVVKVARREFFEWVKSFKDALANKKNKLTIRFFNGDAMSFCKALNYCRVQKSIDTGYYAAPWTTSPLRLDGGDYNEDSENRAPVMFNAIDTSNLTDHLGLINLLLMTTPLLIRSPLSSVYSETKFRPGNTPIDALSDRICGDIPAVSLLFDLAPISYLMHVNSRSSTHAVLLTLVGDPKDGGDGTFQERITWKLASLSDPLAVKVDGIQTKILEYESTQLARFIFGVYQKMFKPDEHMKDINLIHYTRGTLVLLLKLVKDRVKTDWDDFIRQFNELVVSDTNLSLGKSNYQELAAQLHVMGLHTFDFLGNYRQVYPDAQPNVGVFKDWKDVPPVVCIVLTVSRGALKPLIETGGGAGNPFLHCETWNRGGQNYYSCFHPVFGKARREGDRVVIDEAPDGWHGTSPLVVSFWVPSWTLITHPNDTIVAFRAVPMAPRTTGALALLLRLGQMMRLSETNLISKNVIVTRTRPYISSEMDKVRTLSFQRETFVPSPLRSVEISPVRVKLDESCQKIVSLTIRGDLLVKIAKDKLSSGAEVTVIQSSPCTLQVQFGGLRQTMAFPFPVDVAQAKTKIARTSSYIEVSPHYQIQQHVPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5G1</accession>
    <name>D5G5G1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00004320001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN429998">
        <property type="protein sequence ID" value="CAZ79754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835597.1">
        <property type="nucleotide sequence ID" value="XM_002835551.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5G1"/>
    <dbReference type="GeneID" id="9183740"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004320001"/>
    <dbReference type="HOGENOM" id="CLU_1644952_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5G1"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ79754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="161" mass="18176" checksum="F797B100AC8FAD82" modified="2010-06-15" version="1">MAYCAIVAKNPLWFLSRVKKNISRNALAIVIDFQFSSPIQPSLSKDSPQTTLFHSSLAIARVSLRSNSLSYPEHHSRAFIFGDCCLLQQLSRQLTPDRARSSYQHLVALPLRVCGFEVLLYIPPPQPPTVPWAALSKLYQRHLPPPPQSPGLAQHVFRRHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5S6</accession>
    <name>D5G5S6_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA polymerase II holoenzyme cyclin-like subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00001461001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family. Cyclin C subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430001">
        <property type="protein sequence ID" value="CAZ79869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835712.1">
        <property type="nucleotide sequence ID" value="XM_002835666.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5S6"/>
    <dbReference type="STRING" id="656061.D5G5S6"/>
    <dbReference type="GeneID" id="9187547"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001461001"/>
    <dbReference type="eggNOG" id="KOG0834">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022000_7_0_1"/>
    <dbReference type="InParanoid" id="D5G5S6"/>
    <dbReference type="OMA" id="MYDMMKY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20545">
        <property type="entry name" value="CYCLIN_SpCG1C-like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20546">
        <property type="entry name" value="CYCLIN_SpCG1C_ScCTK2-like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043198">
        <property type="entry name" value="Cyclin/Ssn8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10026">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036580">
        <property type="entry name" value="Cyclin_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0195">Cyclin</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="5">
        <location>
            <begin position="42"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="5">
        <location>
            <begin position="155"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="259"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="284"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="358"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ79869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="367" mass="41507" checksum="81F13BF8E204E02F" modified="2010-06-15" version="1">MSERQNLESQWIFTEEELLRTPSVLDGISPELEREQRGKGCNFILQMGIQLKLPQLTLATASVFLHRFYMQNSLKKHHYYETAATALFVATKVEENMRKFGELVAACVRAAQKNHALEVHRDDKEFWKWKDCILTKEDYLLESICFDLSVEAPYNLLLQYTNKLGVQTRQLIRTAWTFINDSTLTMLCLLYPSKTIAAAALYCAAKHCGIEFPDQDGKAWWDVIGVSIKDIKRACNYMAMVYEKNPLRGEDGVRYVATPENGAETTRTRVLAVNNSSPPAESVSRSRTESRSGSEGGKRMRDQSRTDEEISEEAVKKKLKLHEEAVGNGVEVPENNSSMRGKEKAPPPPPPPEKPESGELEEGEVDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5W9</accession>
    <name>D5G5W9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00001606001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430002">
        <property type="protein sequence ID" value="CAZ79912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835755.1">
        <property type="nucleotide sequence ID" value="XM_002835709.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5W9"/>
    <dbReference type="STRING" id="656061.D5G5W9"/>
    <dbReference type="GeneID" id="9187590"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001606001"/>
    <dbReference type="eggNOG" id="ENOG502SD2N">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_326523_0_0_1"/>
    <dbReference type="InParanoid" id="D5G5W9"/>
    <dbReference type="OMA" id="TFRECFF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="466"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ79912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="910" mass="102150" checksum="4F535FD23FEF8181" modified="2010-06-15" version="1">MSGDESDSPPKETYIAYKIKTIFEFTPGAIHKLLRTYKEKQSRQLIPVDCFFDSKLKLFVLWGDQEACYGAFKTFMDVLSVAAKDAITTIRYGDKVEAPIKRSPFSHLNLGAVQWTEDVSDDDEPDSHDERGDDDFLLPVSSKDLVTSSSKPIAEHKYTETWDPKLSTLDFSYVLHPVDGYHFIKQISRVTGCTLSPDKVKKVVKIGAGSPASIKLALKKLRKVLERYCRGFESIEATLVNPDEKKKFEIRLVSLEKQSELNRTTLYPHGSKWAEIPVENKFLIRMAEYSPEAGKFLNCKIEPRSLQERFACLTDPRHRWERYLESRGVWDGDAGDSLAPSTFILGSALNRAGGFLMGPESNSGDDDGTPTLDLCDPQLNFDSRLGTQPPYEMRANAAPTVKPTIRTVRAKKSAEQEKGIGFAIKGNSDLLDGEITLESPTLSPTPAPTRNQDHVFSFEGAFSEIQRPPPTVIPTTRTVRKPRAKKTDAGAPEVGTKTLHRTMNQQAPVRTKEGRSDLTDGVDDSKRYALGCIVARIKVHSVSRVTKYNVQKIQKIFKSWYGFTFLSVTVANFAISFTHVRGWRGHVKFEASLGRIVLTGVPRTISRRGIEWGDWESSVEKLKEIKTHFTSVYAHLLLFSGGTQYLPFYRVTREAHDVEHVLDLKLSRFEAMFSGDLRSEEITYEFHCDVESKNRIFVMNAQTFDVTCYNDVSDFGTVYWAHPTRAWDARIRLAGCKVLDLETPGFAEFLASISVRGGGSPPAVGFDVKGTGITVNKVQYRHMLRYQVNKKVCLGHEIDLAVTELQDLQVNRRSGPGRRYEAISTGKPAMAADERLSYLFSLVPVEIEKHLVENYNLEVGEEVTWNTEVLADRGGGDGILKALISVVNTLVQKTDDVGFDNNNMPYQRSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>D5G5Z3</accession>
    <name>D5G5Z3_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Ribokinase</fullName>
            <shortName evidence="12">RK</shortName>
            <ecNumber evidence="2 12">2.7.1.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">GSTUM_00001691001</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the phosphorylation of ribose at O-5 in a reaction requiring ATP and magnesium. The resulting D-ribose-5-phosphate can then be used either for sythesis of nucleotides, histidine, and tryptophan, or as a component of the pentose phosphate pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>D-ribose + ATP = D-ribose 5-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13697"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:47013"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.15"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="12">Requires a divalent cation, most likely magnesium in vivo, as an electrophilic catalyst to aid phosphoryl group transfer. It is the chelate of the metal and the nucleotide that is the actual substrate.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="12">Activated by a monovalent cation that binds near, but not in, the active site. The most likely occupant of the site in vivo is potassium. Ion binding induces a conformational change that may alter substrate affinity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="12">Carbohydrate metabolism; D-ribose degradation; D-ribose 5-phosphate from beta-D-ribopyranose: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the carbohydrate kinase PfkB family. Ribokinase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the carbohydrate kinase pfkB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.15" evidence="2 12"/>
    <dbReference type="EMBL" id="FN430005">
        <property type="protein sequence ID" value="CAZ79936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835779.1">
        <property type="nucleotide sequence ID" value="XM_002835733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G5Z3"/>
    <dbReference type="FunCoup" id="D5G5Z3">
        <property type="interactions" value="1029"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G5Z3"/>
    <dbReference type="GeneID" id="9187614"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001691001"/>
    <dbReference type="eggNOG" id="KOG2855">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027634_2_0_1"/>
    <dbReference type="InParanoid" id="D5G5Z3"/>
    <dbReference type="OMA" id="DIVLIQQ"/>
    <dbReference type="UniPathway" id="UPA00916">
        <property type="reaction ID" value="UER00889"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004747">
        <property type="term" value="F:ribokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019303">
        <property type="term" value="P:D-ribose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01174">
        <property type="entry name" value="ribokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01987">
        <property type="entry name" value="Ribokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002139">
        <property type="entry name" value="Ribo/fructo_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011877">
        <property type="entry name" value="Ribokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10584:SF166">
        <property type="entry name" value="RIBOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10584">
        <property type="entry name" value="SUGAR KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00990">
        <property type="entry name" value="RIBOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00584">
        <property type="entry name" value="PFKB_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 12" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 12 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10 12" id="KW-0630">Potassium</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase PfkB" evidence="14">
        <location>
            <begin position="7"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="276"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="15"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="43"/>
            <end position="47"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="241"/>
            <end position="246"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="275"/>
            <end position="276"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="311"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAZ79936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="323" mass="34563" checksum="EB6A4255DB92F7B6" modified="2010-06-15" version="1">MAPIPRTILVIGSLNIDLTTRTRRLPNPGETIAAQTFSTAPGGKGANQAVACCRLARASSDAPLDNILVKMVGAVGDDEFGGRLKDALRQNRIDVEGVVVKGGVSSGVAVIIVDEDTAENRILYTAGANEALTAQDFPENFFSSFTPLPSFLVLQLETPLQTVLHLLTLASNAGVETVLNPAPARHIPLQFYSKIDHLILNESEAAYLTGEEVTFLDDDDGVKLTIDRFLERGVKRSVVITMGARGCYFKSPDRQEGWIRPEVRAQDVVDTTGAGDAFVGAFAVARVEGNDVGQSLNFATRAASIKVQNMGAQAGIPWRGEVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>D5G682</accession>
    <name>D5G682_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00001650001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430008">
        <property type="protein sequence ID" value="CAZ80025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835868.1">
        <property type="nucleotide sequence ID" value="XM_002835822.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G682"/>
    <dbReference type="GeneID" id="9187704"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001650001"/>
    <dbReference type="eggNOG" id="ENOG502QQNH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1397250_0_0_1"/>
    <dbReference type="InParanoid" id="D5G682"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ80025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="195" mass="21644" checksum="358626AF6533FB79" modified="2010-06-15" version="1">MGLLVQTKIPQWTAKPQQLGSLGRIIPRGREKVLKNLKMHDPGRSLLGEFSAIRRRRETQESVLRVNRAKEGSKEFASDDPRARQLGRTLSDGFAVIKEKYRAPDNPIVLCHGLLGFDELRLAGNYLPGIRYWRGVTEAFNANNIEVILTSVPASGSIEVRAKALAECIRLRAKGRNVNMIGHSMVGFGVGDRGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6F0</accession>
    <name>D5G6F0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pre-mRNA-splicing factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00004453001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Involved in mRNA splicing where it associates with cdc5 and the other cwf proteins as part of the spliceosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Belongs to the 40S cdc5-associated complex (or cwf complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CWF11 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430009">
        <property type="protein sequence ID" value="CAZ80093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835936.1">
        <property type="nucleotide sequence ID" value="XM_002835890.1"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G6F0"/>
    <dbReference type="GeneID" id="9181178"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004453001"/>
    <dbReference type="eggNOG" id="KOG1806">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001195_0_0_1"/>
    <dbReference type="InParanoid" id="D5G6F0"/>
    <dbReference type="OMA" id="YRVWLDC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005684">
        <property type="term" value="C:U2-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd17935">
        <property type="entry name" value="EEXXQc_AQR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000507">
        <property type="entry name" value="Pre-mRNA-splicing factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048966">
        <property type="entry name" value="Aquarius_b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048967">
        <property type="entry name" value="Aquarius_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032174">
        <property type="entry name" value="Aquarius_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026300">
        <property type="entry name" value="CWF11_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045055">
        <property type="entry name" value="DNA2/NAM7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887:SF5">
        <property type="entry name" value="RNA HELICASE AQUARIUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16399">
        <property type="entry name" value="Aquarius_N_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21143">
        <property type="entry name" value="Aquarius_N_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21144">
        <property type="entry name" value="Aquarius_N_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038901">
        <property type="entry name" value="AQR_cwf11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0347">Helicase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="2" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNA helicase aquarius N-terminal" evidence="6">
        <location>
            <begin position="28"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="RNA helicase aquarius beta-barrel" evidence="7">
        <location>
            <begin position="494"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="domain" description="RNA helicase aquarius insertion" evidence="8">
        <location>
            <begin position="709"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="4">
        <location>
            <begin position="807"/>
            <end position="1105"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase-like C-terminal" evidence="5">
        <location>
            <begin position="1116"/>
            <end position="1308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="733"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="769"/>
            <end position="779"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR038901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF16399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF21143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF21144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ80093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1386" mass="158546" checksum="7DF264C45A35E246" modified="2010-06-15" version="1">MEKPDKTMVGAVEEARPTVADLHGDNLFAQAAKKYWLTEKPVRFKPEALKAEFYDPLEKDGFRFRSLLILENLQFLEKLLWPNFTETASNYHVVLIALMVNVKRRENLKIWEYISLLPDHFPPLFRRILFMTIDRGTPQPIRNHLLTFIVTAFQSLDSGLVRKECAPLVSISIWNNLSSEEVRNARLAKYPQTKKAWRAAGKRFDSADDSTKDRLRFDRLWLFEMILDFLNMMYKPLDQQEGDILNYCERFIELLTDLESQMPTRRYVNFLLKDLHVLTAIKLSPLYTSSENGLFRDLYALLFHYINFSIDDHTGLQLSQEESRQIHNADLAKLQRTAFKHFKDKLTILALANYGSIGQRDELASHIAELGDGEVVELCHLLGLRTEYPQKVVMSVDRAFLTEAILLCHEKRKTFQEQAKELPVLPNQDTLFESSLLRNEYYDGSGPLAIPKLNLQYLTVGDFLWRSFILYRCEAFFAIRRDIEDSLHRLAPKLLHPSMTTQFHGFSKMALLITRPSILETAPPKVGEEKPAYVRAEISLDYARLSEDVRRDWDSLRPDDVVFLLAVKGVDEGDRVITNGSPDKLNTAEKFGVKYLRAAEVIQLLDQDGRLIKAGDGQARRAGGRRRLHVRLDPEMYKIDADDLKAGKPDAYDSINVLIRRKGRENNFKPVLESIQELTQADVPMPIWLQEVFLGYGDPAGAHYSKLPNQPRLVDFRDTFLDWQHLVESFPGKKLKPKEGSQEGCDPPYVISQKTAAEQPKPARKRRRGQEDNPDKEESLEVSTYKLPNMGPYPIDVPRSNHIKFTPTQVEAIRSGTNPGLTVIVGPPGTGKTDVATQIISNLYHNFPAQRTLLIAHSNQALNQLFEKITALDIDERHLLRLGHGEEELKTDTNYSKQGRVESFMDNRVKLLAEVDRLAATLGAPGAHGDSCETADYFNLVYVKPAWARYQKVLSEGGLALEKLYDSFPFHQYFSNAPKPMFSQGLSLEEAIEVAEGGYRHIGKLFTELEDIRPFELLKTSRDRQNYLLTKEARIVAMTSTHAAMKRADIAKLGFEYDNVVMEEAAQITEVETFIPLALQSPRGGELPLQRVVLCGDHYQNSPIVQNIAFRQYANLEQSLFARLVRLGAPTVNLDQQGRARASISRLYNWRYRELGNLPLLESQPEYQTANAGFRHEFQFINVKDYKGQGESEPTPHFIQNLGEAEYAVAIYQYMRLLGYPAEKITILTTYLGQRSLIRDVLNRRCAKISLFGMPASLTTVDKYQGEQNDYVILSLVRTNRVGYLRDIRRMTVALSRARLGLYVVGRREVFESCYELSEAFSRLLENKSDKLEIVTGEMWPTDRREKTKEGTEMENVEHMGQYVYEMTKTRGEHEAGQAVAASLVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6F9</accession>
    <name>D5G6F9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00004465001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430009">
        <property type="protein sequence ID" value="CAZ80102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835945.1">
        <property type="nucleotide sequence ID" value="XM_002835899.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6F9"/>
    <dbReference type="GeneID" id="9181187"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004465001"/>
    <dbReference type="eggNOG" id="ENOG502SRM2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1278449_0_0_1"/>
    <dbReference type="InParanoid" id="D5G6F9"/>
    <dbReference type="OMA" id="PPKETFN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021641">
        <property type="entry name" value="DUF3245"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11595">
        <property type="entry name" value="DUF3245"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="200"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="216" mass="23698" checksum="169AB5717C8BA5FE" modified="2010-06-15" version="1">MSTPTSTFTPTSTSETHQARISLHLSHTRSLVSSWLPPPPTDLAAAQKPEEEIFREDEEFFAPIPPRLGLGAPIPREFLNGESRVRTNDLLKKKIMGRGGGGRGGSKPMPEGSKRKEGGVVWSDEEEEGRSALGRKKRVKVLQEKEKEYFNNSGVTIASSATLTADTPPPLAPPKETFNKRSPTPPTRFGTFLDAYQAQKAKKKKKKRKSASTGQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6G5</accession>
    <name>D5G6G5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00004472001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430009">
        <property type="protein sequence ID" value="CAZ80108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002835951.1">
        <property type="nucleotide sequence ID" value="XM_002835905.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6G5"/>
    <dbReference type="GeneID" id="9181193"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004472001"/>
    <dbReference type="eggNOG" id="ENOG502SF0B">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1415112_0_0_1"/>
    <dbReference type="InParanoid" id="D5G6G5"/>
    <dbReference type="OMA" id="EREYLWD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="188" mass="21476" checksum="0D14B85F5892C911" modified="2010-06-15" version="1">MASDLPVSDLTADLRGPAVERQRGKERRIKYRNVNMALLGQPKTLFWPDGTKPAPVWNDRNKFRMPQQDSRSCHCHGCRSWRAREQNKKKAVEKLHWDDEICTARQYPLSPSAEEREYLWDDSSSDSGSIEGVAYSFDRPGPSTGTDTLSYAVTEAVKKFENKELATLIKNEYEVIDASDSDEDFELV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6M0</accession>
    <name>D5G6M0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cullin-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">GSTUM_00002124001</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">Mel28</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11 12">Belongs to the cullin family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430012">
        <property type="protein sequence ID" value="CAZ80163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836006.1">
        <property type="nucleotide sequence ID" value="XM_002835960.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6M0"/>
    <dbReference type="FunCoup" id="D5G6M0">
        <property type="interactions" value="983"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G6M0"/>
    <dbReference type="GeneID" id="9187227"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002124001"/>
    <dbReference type="eggNOG" id="KOG2166">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004747_6_1_1"/>
    <dbReference type="InParanoid" id="D5G6M0"/>
    <dbReference type="OMA" id="IREWDRY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902531">
        <property type="term" value="P:regulation of intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000014">
        <property type="entry name" value="Cullin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000007">
        <property type="entry name" value="Cullin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000011">
        <property type="entry name" value="Cullin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000026">
        <property type="entry name" value="Cullin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.130:FF:000003">
        <property type="entry name" value="Cullin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000029">
        <property type="entry name" value="Cullin homolog 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1310.10">
        <property type="entry name" value="Cullin Repeats"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.130">
        <property type="entry name" value="Cullin, Chain C, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045093">
        <property type="entry name" value="Cullin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059120">
        <property type="entry name" value="Cullin-like_AB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016157">
        <property type="entry name" value="Cullin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016158">
        <property type="entry name" value="Cullin_homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036317">
        <property type="entry name" value="Cullin_homology_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001373">
        <property type="entry name" value="Cullin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019559">
        <property type="entry name" value="Cullin_neddylation_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11932">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00888">
        <property type="entry name" value="Cullin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26557">
        <property type="entry name" value="Cullin_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10557">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00182">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00884">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75632">
        <property type="entry name" value="Cullin homology domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01256">
        <property type="entry name" value="CULLIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50069">
        <property type="entry name" value="CULLIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Cullin family profile" evidence="13">
        <location>
            <begin position="409"/>
            <end position="643"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00069612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="CAZ80163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="768" mass="88026" checksum="D50DF866675F98BF" modified="2010-06-15" version="1">MQPKAPNREDLGQTWSYLEAGVEKIMKNLQTGVDMTTYMGVYTAVHNFCTSQKAVNSSPQALHNSGAVHRGGAVAHLLGEDLYKNLIGYLSKHLTALKDEASGHADEALLALYIRDWDRYTTAAKYINHLFRYLNRHWVKREMDEGKKNIYDVYTLHLVRWRVDLFDHVQKYVMDGVLKLVEKQRNGETIETAMVKSIVDSFVSLGLDESDSSKSTLDVYRQFFEKPFLEATTAYYQMESKQFVAENSVVEYMKKAETRLAEEEGRVQMYLHPDIYGPLMKTCQKVLIQEHAPLLREEFQVLLDNDRQSDLQRMYNLLSRIPDGLEPLRTKFEAHVRRAGTSAVDKIADEGGDNLDPKVYVDALLEVHTQYQNLVNVAFKGEAEFVRSLDNACREFVNRNKVCKSASTKSPELLAKYADSLLRKSAKSAEESDLENKLTAIMTVFKYVEDKDVFQKFYSKMLAKRLVNFTSASDDAETSMIGKLKEACGFEYTNKLQRMFQDMQISKDLNDNYKAWLEGKGESASNGVDFSCQVLGTSFWPLNPPTTPFNIPEVIVQTYTRFVEFYNGKHNGRKLTWLWHLCKGELKASYCKATKTPYTFQVSTYQMAMLLLFNDATKISYEEFEKSTGLSKEYMEPALAVFLKAKVLTISPPGSKIGPGTQYSLNFDFKSKKIRVNLNMAVRAEQKQEVEETHKTIEEDRKLLMQSAIVRIMKARKVLKHVVLVQETIGQIKSRFTPKIPDIKKCIDILLEKEYLERLDGERLGYLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6P5</accession>
    <name>D5G6P5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00002154001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430012">
        <property type="protein sequence ID" value="CAZ80188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836031.1">
        <property type="nucleotide sequence ID" value="XM_002835985.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6P5"/>
    <dbReference type="STRING" id="656061.D5G6P5"/>
    <dbReference type="GeneID" id="9187214"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002154001"/>
    <dbReference type="eggNOG" id="KOG1001">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000315_2_7_1"/>
    <dbReference type="InParanoid" id="D5G6P5"/>
    <dbReference type="OMA" id="YYVFHGP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18008">
        <property type="entry name" value="DEXDc_SHPRH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050628">
        <property type="entry name" value="SNF2_RAD54_helicase_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626:SF52">
        <property type="entry name" value="SINGLE-STRANDED DNA-DEPENDENT ATPASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="4">
        <location>
            <begin position="238"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="5">
        <location>
            <begin position="701"/>
            <end position="859"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ80188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="876" mass="96339" checksum="8AFDC3CB65564C13" modified="2010-06-15" version="1">MYVTAHSLPAPIPLEDRFTTHWPRIPVSLKCHGGGSSLSVGVIDPFGKEFGSLDTNSARVLVPLLERNLVRLQTIVLPRKKQPGHLPGPETDGKCKTPLEMQANVYGPVMNMGAIAKILADKNGRLKRPSQTDGLEYVPPMGTPGVSRVLPVQVDTEAAVFTMLEELQKQVENLPNAEADPRITTPLLKHQKQGLHFMMSKERDRDYTDSKGNTSLWRTFGHGSPTFYENVITCDQREEKPDEVYGGILADVMGLGKTLQVICLIVGSLDAATAFAAPAEGERPSKRRRVKTTLVVSPLSTIGNWEGQIKAHVKFGTLSVYVYHGPKRVLSIEKLAQYDVILTTYQIVGGEFAKHTTGGGGASASKGSCPFQKLHFFRIVLDEAHMIRSPSIMLTRAMLSLNAQRRWAVTGTPIQNRLGDIATLVKFLRIAPFDDSTAWNKYIAAPFKNANIESIANLRRILHSVTLRRSKGIINLPPRKDEVVFLDFSSSEQQLYEATLRMSRRKLDLVLRDGHIGGQNYVHVLQSILRLRLICAHGSELVGDSDTAGITSSHAINVDEIGGNASDLPWSVKDGYQIFRLMYDANDDICALCEAKVGINSTAGSVGDEDSPSNKKVVVIGHLTACAHLLCKTCGPRFTEEFNNASISAAGNKPLHGDCPLCGACVSSALLDIKSDYNEDSVQDLQRRKKLKGRYGGPSTKVKALISSLLENKKASTTSSPIKSVVFSCWTSHMDLIEIAFKDNGINFVRLDGSMTRTQRNRVMEDFERAPEISVILISIMAGGLGLNLTAACKAYVMEPQFNPAAESQAIDRIHRLGQTRPVTTTRYIMRDSFEMKIVELQKKKTELANLSMSSGRLSGKDAMAKKLEDLKTLFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>D5G6X5</accession>
    <name>D5G6X5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00002373001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sorting nexin family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430019">
        <property type="protein sequence ID" value="CAZ80268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002842533.1">
        <property type="nucleotide sequence ID" value="XM_002842487.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G6X5"/>
    <dbReference type="FunCoup" id="D5G6X5">
        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G6X5"/>
    <dbReference type="GeneID" id="9184082"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002373001"/>
    <dbReference type="eggNOG" id="KOG2101">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002131_1_0_1"/>
    <dbReference type="InParanoid" id="D5G6X5"/>
    <dbReference type="OMA" id="AMYVVEV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06876">
        <property type="entry name" value="PX_MDM1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003114">
        <property type="entry name" value="Phox_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013937">
        <property type="entry name" value="Sorting_nexin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22775">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22775:SF3">
        <property type="entry name" value="SORTING NEXIN-13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08628">
        <property type="entry name" value="Nexin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02194">
        <property type="entry name" value="PXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00313">
        <property type="entry name" value="PXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51207">
        <property type="entry name" value="PXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072618" evidence="4">
        <location>
            <begin position="22"/>
            <end position="1230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="249"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="PXA" evidence="7">
        <location>
            <begin position="99"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="RGS" evidence="5">
        <location>
            <begin position="435"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="domain" description="PX" evidence="6">
        <location>
            <begin position="893"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="586"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="694"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="790"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="592"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="602"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="624"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="656"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="701"/>
            <end position="721"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ80268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1230" mass="137483" checksum="0D4FA1BAA38C7ECC" modified="2010-06-15" version="1">MHRRSLAITAVILLLLLNTTAQFFPSLRLIVWAFFTGSTASIILLLVSGLFTVHRRPSDNNVLKTIKPLAFASPALWSKEVEAIALEDSFYRLPLYPPSFVLSDSIDVFLENVLRDFVSSWYSKISSDPSFTIQVDRTIRHALGVLRDRIQGLDLVEVIVGKIVPLLTAHLHDFSAAERAVRGKHLNKDLTESEELDLAIAGKYREGKLHPAASLKFSDTKLAQQDHLRLLVDNILPVLLPERERKSRAVATIVKEIVACGILFPVMGILSDPDTWNQFIEAIGRSTLQDRKTVRKLRAALDQHATPRHPNSLGHSRNYSQVELAPSQPFIRLSAKDDERTFERFIRSIRLCNNLSDARRLRNEVSAQLKRDERVEPKEGWAVYLRRLETGKREVDKRVAQLSASVAGPGGGPIRTHSEVLVPKQATTSHLEFATPQQVLRDSSGLSYFMEHMDRRRRLPLVQFWLVVDGLRNPLEEDVSSSDDEQETSTSTAHAAWTLSDRADLAQIHEAYLSMPELQIRERTNLAVKEFLKAGSNATQEQYIRARAAVLRAQTAVFEEMQQKDFPEFRASDLFYKYLASDETAASQVRPVSDRTSREMVRSSSWANASHPSETSDPMISSRHSLDGARPAERSLDDWDFDPLSNSRQSLDEEPPQYSQYLSQPQQEPGRHSPEDGIVEAMEAALNDIIEDRPSLDESRSSPFQNSPVLTGPNSPRSSIDVSRDSNSNGTGSGNGNGQKFTAVSAVKEKAKAKPPSISSLGLVGTGPSGGVFADDELFPDEAAVVDCHSSGEEAEDKDGPDGGSGSDDEIRQAAPGDLGLAEAIAALTFDIEKLSTQEAVVDSLYRKAELTNNTVELRILGKSKASLHREIRRKELQRQQYIVQESDNSLYGRAKIRIKSTRVGRENGQEYALYIIEVHRPAGDQMPPATWTVPRRYSEFLNLHQTLRSTFPSVKPLDFPRRRVVMKFQKDFLEKRRVSLETYLCSLLLLPDVCRSRTFHYRRDIMSRLYNSIADGVEDLQHQSNPPSIIPGETIDAAEAEAELSAFENRESTPFVKPICDIFLEVFQLNRGSNWLRGRAVVVVLQQLLGGTIERKVREQVRGLVDEEEIIRYLDMLKATLWPEAVATGQQQQQAGRGAKEKVRARNEAKIILATLIPDLAGSVVGRENARAAGRRLFAGLNNGRLKCVPPFSSLDKVGWADVCVVGFSSSIVYTILDELVGIVFDERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7E6</accession>
    <name>D5G7E6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00002194001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430025">
        <property type="protein sequence ID" value="CAZ80439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836248.1">
        <property type="nucleotide sequence ID" value="XM_002836202.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7E6"/>
    <dbReference type="GeneID" id="9188464"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002194001"/>
    <dbReference type="eggNOG" id="ENOG502RZ72">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035633_0_0_1"/>
    <dbReference type="InParanoid" id="D5G7E6"/>
    <dbReference type="OMA" id="AWGFEDP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="174"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="204"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ80439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="308" mass="33913" checksum="6D4970A3292A0EEC" modified="2010-06-15" version="1">MKPKRQRISGTSPTSAVLSLDECHHPGPLRSPQPPVRSSQLHSAPASCPQDISEHDKVVFAPVTDLAFELISHPATFISPKVLSAYVTLQSVIKDPTPIPAVFSFYARKSYIPPGTAKVISPNANQTVNAVPRDVADAALSIAIEAKDMALCLDIIDTSYALPAFRRAKLIREATPMAILASLMPPVAYVFGDILARYQDRLDYSHALGFSFAGILAYITFTAGLGFVAVTTANDQMARVTWAVGTPLRLRWLREEERAAYDRVAQAWGFEDPVKRGFEEGEAWELLREVVFRKNMILDNPDLMDGME</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7M8</accession>
    <name>D5G7M8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00004638001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430031">
        <property type="protein sequence ID" value="CAZ80521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836330.1">
        <property type="nucleotide sequence ID" value="XM_002836284.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7M8"/>
    <dbReference type="GeneID" id="9187000"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004638001"/>
    <dbReference type="eggNOG" id="ENOG502S5YS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100672_0_0_1"/>
    <dbReference type="InParanoid" id="D5G7M8"/>
    <dbReference type="OMA" id="PIEWQDR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013869">
        <property type="entry name" value="DUF1757"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38636:SF1">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN CLC-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38636">
        <property type="entry name" value="PROTEIN CBG20488"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08560">
        <property type="entry name" value="DUF1757"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ80521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="174" mass="18723" checksum="F90C48A8F3C563F9" modified="2010-06-15" version="1">MDRFFLRHDYAEDQPYSRTILTTHVLHRGANLGAVFGALYGGARYGLSTHARSLPLVASIVRASGVGVVVTTGITAVMLSARMYGKQEIEWQDRSWRLLENKPQNRIDEWSASGALVGGVLGGVKKGLGWRGITGSAGIGSVVGIVGWAVYNKTRGPGEQVKVAKNLEKTIVKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5G7N0</accession>
    <name>D5G7N0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glutamine synthetase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00004641001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the glutamine synthetase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430031">
        <property type="protein sequence ID" value="CAZ80523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836332.1">
        <property type="nucleotide sequence ID" value="XM_002836286.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G7N0"/>
    <dbReference type="STRING" id="656061.D5G7N0"/>
    <dbReference type="GeneID" id="9187002"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004641001"/>
    <dbReference type="eggNOG" id="KOG0683">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017290_0_1_1"/>
    <dbReference type="InParanoid" id="D5G7N0"/>
    <dbReference type="OMA" id="NIMTFRL"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamine synthetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006576">
        <property type="term" value="P:biogenic amine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006542">
        <property type="term" value="P:glutamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.70:FF:000013">
        <property type="entry name" value="Glutamine synthetase bacteria"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.10:FF:000005">
        <property type="entry name" value="Probable glutamine synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.70">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.10">
        <property type="entry name" value="Glutamine synthetase/guanido kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036651">
        <property type="entry name" value="Gln_synt_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43785">
        <property type="entry name" value="GAMMA-GLUTAMYLPUTRESCINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43785:SF12">
        <property type="entry name" value="TYPE-1 GLUTAMINE SYNTHETASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01230">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51987">
        <property type="entry name" value="GS_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GS catalytic" evidence="8">
        <location>
            <begin position="121"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ80523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="467" mass="51924" checksum="959910CA93ED1D9A" modified="2010-06-15" version="1">MAAPYTLESITAALSGDDKVKLAGVDVDGIHRGKLISKKKFLSIAESGFGFCSVIFGWDMHDRSYFRELGISNSANGYRDLLAKVDFQSFRRIPWEDQRGVPFFLISFYDPDTKEPIAPCPRGLLFNVARKFEEKGWSAMAGAEYEFFNFREDPHSLVANKGSTLEHMTPGMFGYSLTRPVQNQDFYYGVFNACKNFRVDIEGWHTESGPGVYEAALEFGEVKETADRAGLFKYVVKSLGSQYGITPCFMAKPKEGLPGNSGHMHISLVDSDGNNLFARSECDPEAGRDLAYLSDIGRHFLAGVLEGLPSIMPLLAPTVNSYKRLVENFWAPVTVSWGLEHRAASIRLISPSLASPKATRFEIRTCGADANPYYVLAAVLSTGLRGVEKKLEIPVSPLGVGEETGGGERLARNLMEAVKKMAAKGSVAREVFGDAFVDHYTGTREHEWRLWEEAVTDWEIRRYIETV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>D5G836</accession>
    <name>D5G836_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00002793001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIP5 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430039">
        <property type="protein sequence ID" value="CAZ80679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836488.1">
        <property type="nucleotide sequence ID" value="XM_002836442.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G836"/>
    <dbReference type="FunCoup" id="D5G836">
        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G836"/>
    <dbReference type="GeneID" id="9184152"/>
    <dbReference type="KEGG" id="tml:GSTUM_00002793001"/>
    <dbReference type="eggNOG" id="KOG2789">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009068_2_0_1"/>
    <dbReference type="InParanoid" id="D5G836"/>
    <dbReference type="OMA" id="FSTSWAE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd24139">
        <property type="entry name" value="SIP5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039301">
        <property type="entry name" value="Sip5/DA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31315">
        <property type="entry name" value="PROTEIN SIP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31315:SF1">
        <property type="entry name" value="PROTEIN SIP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="171"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="321"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="377"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="456"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="542"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="603"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="25"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="46"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="76"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="216"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="232"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="329"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="377"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="456"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="542"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="566"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="618"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="649"/>
            <end position="662"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ80679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="662" mass="70867" checksum="8C07975D1ACE420C" modified="2010-06-15" version="1">MGNTSTKERSLTPPHGSHHQSSANLPSSSRRGGPSGTGRLSNTLLGGPGDNGGGAGGGYSGRGRSRHGDLFGLNGRETRERDREAEREARELRKAERERERLKERERSLREESVDGGFLVTQGVYTGVEDFRDKIVRQLMIERRLAPFFKGLSDHDSNWTDSQLVAAVKGLPIPSPSATPSIHDPLQSPTPPRSPGPSDSTNNLTIPIATRSRSHSYTSETSNRSETSTPTLNFGSRSRAKTISISSPHNPTLASIASPLETNDPSRIVDGRPIEAVLYKSAVECPICFLYYPPYLNKTRCCDQEICSECFVQIKRPDPHLPEHHDDPNNPSSSNSSPDQLISEPATCPFCVEPEFGVIYSPPPFWRGIQYVTNTATTSSGSNSTSSTPPQTVPLSPSHPPQSPGRRPVLSVTSPEVVTTDRIRPDWSQKLATARSHAARRAAAATALHTAAYLMSPSPASSSSSATPGNNPPSTFSSRRLIRRAQAELLAPSQPPPPSTLASSSNNTSNAIATDYLSRRSRMVDLEEMMLLEAIRLSLQEEEQRKNKPAEGKGKSVERTDSSDTAAKAGCSSGADGDSGAEENASGGLESIFNFRSFEQMIAGQEARRQRPRLCTEEGGGGGGGSDVGKGKGKDGSSSSSGGDAGNVEDLRSAKGKIVDRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8C0</accession>
    <name>D5G8C0_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pre-mRNA-splicing factor CWC22</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Pre-mRNA-splicing factor cwc22</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00004730001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CWC22 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430042">
        <property type="protein sequence ID" value="CAZ80763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836572.1">
        <property type="nucleotide sequence ID" value="XM_002836526.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8C0"/>
    <dbReference type="FunCoup" id="D5G8C0">
        <property type="interactions" value="876"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G8C0"/>
    <dbReference type="GeneID" id="9188365"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004730001"/>
    <dbReference type="eggNOG" id="KOG2140">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006308_0_2_1"/>
    <dbReference type="InParanoid" id="D5G8C0"/>
    <dbReference type="OMA" id="ILTEDMR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000004">
        <property type="entry name" value="pre-mRNA-splicing factor CWC22 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050781">
        <property type="entry name" value="CWC22_splicing_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003891">
        <property type="entry name" value="Initiation_fac_eIF4g_MI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003890">
        <property type="entry name" value="MIF4G-like_typ-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18034">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN CWF22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18034:SF3">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR CWC22 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02847">
        <property type="entry name" value="MA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02854">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00544">
        <property type="entry name" value="MA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00543">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51366">
        <property type="entry name" value="MI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="MI" evidence="10">
        <location>
            <begin position="426"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="13"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="386"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="617"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="25"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="391"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="633"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="708"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="744"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="792"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="864"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="891"/>
            <end position="903"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00069506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ80763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="910" mass="103620" checksum="22BE0872421293D8" modified="2010-06-15" version="1">MDIDEADEVVVQSVIRLPSPSPPPSKRKRSPNSRSRSPRRRRSLPRRPSRLDSFGGVDSGRARERDRQLNDRLRKLDVRAERTEKEVVEKPKFDAKAEYEKLLSMRSGGTYIPPARLRALQAQITDKTSKEYQRMAWEALKKSINGLINKVNVSNIKHIVPELFGENLIRGRGLYCRSIMKAQAASLPFTPIYAAMTAIVNTKLPQVGELLLNRLVVQFRKAFKRNDKAVCLSSTTFIAQLCNQQVADEILAIEILMLLLNKPTDDSVEIAVGLMREVGAHLEEMAPRANNLVFEQFRTILHEAGIDKRVQYMIEVLFQVRKEKYKDNPIVKEELDLVEEEDQITHKTELDDEIDVQDGLNIFKFDADWEEHEELYRKTKAEILGEGSDVGSDEEDEDESSDEEDEEKQEDKKMEILDQSNTNLVNLRRTIYLTIMSSIDFEECCHKLMKITLPPGLENELCSMVIECCSQERTYTKFYGLIGERFAKLNRFWTGLFEESFGTYYDTIHRYETNRLRNIARFFGHQLSTDALGWHVLSVVHINEEETTASSRIFIKILFQDLAEAMGMKRLQERLKDPFLQEAFSGLFPKDNPRNTRFSINYFTSIGMGGVTEDMREHLKNNVPPPTAIVAPSARSESLSSRSSYSSRSGSSSSGRSRSRSRSRSFSRSVSRSPLRTRDGGRKRGRSYSSSRDSRSRSHSYDSYSSRSRSRSYSRSRSRSRSFSNTRPTKRARGRSYTRSITGSRSPSGSLTRSPSSRRSVSYTPPPRKSERARSRSRSYSSAGSYRRRRSATYSSRSRSGSRSPTPPRKSDGNKSRSSRSPPAYRGRSYTPDRSQSRSRSRSRGYGGKGSRYDFSGRNGNRSRGRERYRGDARPRDDDGGDGRRERPLAAGGGYGGRGGGGRVRAADFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8F3</accession>
    <name>D5G8F3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00004795001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL34 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430042">
        <property type="protein sequence ID" value="CAZ80796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836605.1">
        <property type="nucleotide sequence ID" value="XM_002836559.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8F3"/>
    <dbReference type="FunCoup" id="D5G8F3">
        <property type="interactions" value="854"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5G8F3"/>
    <dbReference type="GeneID" id="9186016"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004795001"/>
    <dbReference type="eggNOG" id="KOG1790">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118652_1_1_1"/>
    <dbReference type="InParanoid" id="D5G8F3"/>
    <dbReference type="OMA" id="RCHKCVR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.340.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.370.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008195">
        <property type="entry name" value="Ribosomal_eL34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038562">
        <property type="entry name" value="Ribosomal_eL34_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018065">
        <property type="entry name" value="Ribosomal_eL34_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10759">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01199">
        <property type="entry name" value="Ribosomal_L34e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01250">
        <property type="entry name" value="RIBOSOMALL34"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01145">
        <property type="entry name" value="RIBOSOMAL_L34E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ80796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="114" mass="12993" checksum="31A7E2BD8FED8C70" modified="2010-06-15" version="1">MVSTRLTYRRRNPYNTRSNRVRVIKTPGGKLRYLHIKKRGSPPKCGDCGIKLPGIPALRPREYSQVSKPTKTVQRAYGGSRCANCVKDRVVRAFLIEEQKIVKKVLKEAAAKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8S9</accession>
    <name>D5G8S9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00004822001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="FN430053">
        <property type="protein sequence ID" value="CAZ80922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836731.1">
        <property type="nucleotide sequence ID" value="XM_002836685.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8S9"/>
    <dbReference type="STRING" id="656061.D5G8S9"/>
    <dbReference type="GeneID" id="9186282"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004822001"/>
    <dbReference type="eggNOG" id="KOG0590">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_63_33_1"/>
    <dbReference type="InParanoid" id="D5G8S9"/>
    <dbReference type="OMA" id="CHVMEYC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030003">
        <property type="term" value="P:intracellular monoatomic cation homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343">
        <property type="entry name" value="SERINE/THREONINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343:SF191">
        <property type="entry name" value="SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="308"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="84"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="209"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="210"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="257"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ80922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="603" mass="65651" checksum="E1AECD4F25FEA573" modified="2010-06-15" version="1">MAFMNTSHSDSCPSTCSSSIASLSSLPMTPETYPEPNVGVVPPLDPELSPPLFGLGHADLDSKPRFIIGPGEFEEHHHGCHTVPEEGEDDETASEMHEPLTPTSDSHSGVFISSSTEGFHAVSNPPAIVNTPATTPVVAKPAPSVEPSTTTPRGKLIRHASGKLHGLIRRVHSSSGNGIPLDQSMGPYTPQPTKSKGFFSTRDTPEQFQVGTPVVSGSSTSTCETPIPRPASTFDEKAAGTTRRPSLFPRRSRSNSVNGIKNITGNAITHPATSGAGSKSRKMSNAVPVMDVPVISLSSKYANHSHVPGKSKICGEGVSAIVKVMHKISGPNTQLYAVKEFRKRGRDESKEEYIEKVNSEYCISKSLNHPNIVLTADLCISSSDRWCHVMEYCTGGDLFTLISKSFMKEAEKLCCFKQLLRGFRNKPGIVGSAPYISPEVQNKDGKCPYDARKLDVWSCAMVYFVLSFGGPLWAKASTTDSPHYVKYLECFNKWLARNPGGEMTKDGDYPKFFPTGQFKPATKRLLYRMMHLDPSKRITSQEALADRWVQSIECCNVDDHTDPNVKQVDAGSKSACKQAGKVGVHRLHAHLPIQTGTTFGREY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>D5G8Z6</accession>
    <name>D5G8Z6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00004910001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Gfa family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430053">
        <property type="protein sequence ID" value="CAZ80989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836798.1">
        <property type="nucleotide sequence ID" value="XM_002836752.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G8Z6"/>
    <dbReference type="GeneID" id="9181272"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004910001"/>
    <dbReference type="eggNOG" id="ENOG502S13F">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038839_1_0_1"/>
    <dbReference type="InParanoid" id="D5G8Z6"/>
    <dbReference type="OMA" id="AWLEWWT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016846">
        <property type="term" value="F:carbon-sulfur lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1590.10">
        <property type="entry name" value="glutathione-dependent formaldehyde- activating enzyme (gfa)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006913">
        <property type="entry name" value="CENP-V/GFA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33337:SF40">
        <property type="entry name" value="CENP-V_GFA DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33337">
        <property type="entry name" value="GFA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04828">
        <property type="entry name" value="GFA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="Mss4-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51891">
        <property type="entry name" value="CENP_V_GFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CENP-V/GFA" evidence="5">
        <location>
            <begin position="185"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ80989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="362" mass="40485" checksum="C52EE48F765F887A" modified="2010-06-15" version="1">MSEGTDKQETVTGRCHCQAVTYSFAFTGPLAAFYTHEAFIRRQTGFPINLYIPMNADCDFQFPSDSPNLQSFKYGDKTSFFCKTCGTTLFFTKRSWEPPCILAPTIDVLSGKQLSDYVYIQAHIHLSEAGGEVGGIADIIEDGLPHYMEDAGKYLDSETIAMVERPVVDESQVMSEHCQGVPDVLDGRCCCGALRFSIARPPEDYSSDPTLLRWVKPGGKFAASFCFCTSCRTVSGAPLFGWCFTPTKQLRIYPQSTPRLSVYRSSDKSTRRFCASCGATAFFALDSLNNEMWDIAIGLLPLGKFRSGDWCVWKYDGGDQFVDSYIHGWKDGTMSYERNGEDYFRGLVEQVRFGRNRSGLVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>D5G907</accession>
    <name>D5G907_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00004927001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="FN430053">
        <property type="protein sequence ID" value="CAZ81000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002836809.1">
        <property type="nucleotide sequence ID" value="XM_002836763.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G907"/>
    <dbReference type="STRING" id="656061.D5G907"/>
    <dbReference type="GeneID" id="9181283"/>
    <dbReference type="KEGG" id="tml:GSTUM_00004927001"/>
    <dbReference type="eggNOG" id="KOG0922">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001832_5_11_1"/>
    <dbReference type="InParanoid" id="D5G907"/>
    <dbReference type="OMA" id="FHEVMET"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001075">
        <property type="entry name" value="ATP dependent RNA helicase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF136">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX35-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="5">
        <location>
            <begin position="34"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="6">
        <location>
            <begin position="215"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ81000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="593" mass="66242" checksum="F041A5614E46F961" modified="2010-06-15" version="1">MASADRATESEECLVSLYRPSTILPITRHRDALLYLVENNPVTIVVGQTGSGKTTQLPQYLHQAGWTEEGKVIACTQPRRVAATTVATRVAEEMGVRLGEEVGYSIRFEDMTSPRTRIKYMTDGMLLREALVDPLLSRYSVVMIDEAHERSLSTDVLLGILKKIRRRREDLRVVVSSATLQAEEFARFFGDEGETKIISLEGRCYPVDVLYLEEPTENYVEKAVQTVFDIHLKARSMNIHFLDKLLNEGEACRGSSPTSPATFTAPQLAVFDPAPENHRKVIVSTNIAEASVTIENIVFVIDCGFVKLRTFDPRTGIEALSAVPVSRASATQRAGRAGRTKAGKCFRLYTQRAFNDIMAEMGIPEIQRSNLAPVLLQLKALGIDNVVRFDFITPPPSELMVRALELLFSLGALDDYARLTKPLGLRMAEMPVDPMIAKILLNSVTFQCTDQILSIAAMTSVQNVFISHEGSKKPAESAKPFAPKANKPNAAKWCKDHYLNFRALSRAVSGIRRCLTTGYFAHVARMQPDGSFRSVNGMQLWAHPSSVMFNRKADWVVFHEVVETGKKTFIRDVTVIEKDWLLEYAPGFHQLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9W8</accession>
    <name>D5G9W8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00005108001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430064">
        <property type="protein sequence ID" value="CAZ81311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837120.1">
        <property type="nucleotide sequence ID" value="XM_002837074.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9W8"/>
    <dbReference type="GeneID" id="9188554"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005108001"/>
    <dbReference type="eggNOG" id="ENOG502RRZT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029564_0_0_1"/>
    <dbReference type="InParanoid" id="D5G9W8"/>
    <dbReference type="OMA" id="FWTRREN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040410">
        <property type="entry name" value="UPF0658_Golgi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34391">
        <property type="entry name" value="UPF0658 GOLGI APPARATUS MEMBRANE PROTEIN C1952.10C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34391:SF1">
        <property type="entry name" value="UPF0658 GOLGI APPARATUS MEMBRANE PROTEIN C1952.10C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072660" evidence="3">
        <location>
            <begin position="22"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="186"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="235"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="264"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="302"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="343"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ81311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="368" mass="41930" checksum="B2874277F95BF1F1" modified="2010-06-15" version="1">MFYRPTSTWSWSFLLLAIVQSCVVLGLEGFVFAAFQNNLHQDFINKSKNEFTKAQTRTIPTYLTLFIFGHIFLVVLCYDALRLKNTIQIIGICIFNAALLLYAAIQMDQIHDANTFLTVLGVGPDPNAVGIKENIWPVIQPSLIAIPCVIALSTVLMCVICWKLYDEFAWTIYKHISADLKMKERYLKFQIFIALLKFDFFFFVGFTVQFIVIVASTSNYLKGGGSKLGNTDKDYEFFLTIAVLPLTIAFLVLAAWCTRREKKIPMVFIIIIFFAALAYFIFKLVRMYHGSFVESYKPAKRSLTTFAAITLILIILTIANAIACVMNFDKGLKPHITRRKMTDEEEKHSMTEMTNQAAQPIPSRMTID</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>D5G9Y8</accession>
    <name>D5G9Y8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00003443001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430067">
        <property type="protein sequence ID" value="CAZ81331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837140.1">
        <property type="nucleotide sequence ID" value="XM_002837094.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5G9Y8"/>
    <dbReference type="GeneID" id="9188573"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003443001"/>
    <dbReference type="HOGENOM" id="CLU_3351397_0_0_1"/>
    <dbReference type="InParanoid" id="D5G9Y8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="37" mass="3619" checksum="E8F994660B1A29C6" modified="2010-06-15" version="1">MMIVRGAREVGEMVRNQDGVNVGGQGGGGGATMGGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAH3</accession>
    <name>D5GAH3_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00003583001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430077">
        <property type="protein sequence ID" value="CAZ81516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837325.1">
        <property type="nucleotide sequence ID" value="XM_002837279.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAH3"/>
    <dbReference type="STRING" id="656061.D5GAH3"/>
    <dbReference type="GeneID" id="9187456"/>
    <dbReference type="KEGG" id="tml:GSTUM_00003583001"/>
    <dbReference type="eggNOG" id="KOG0548">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_401670_0_0_1"/>
    <dbReference type="InParanoid" id="D5GAH3"/>
    <dbReference type="OMA" id="WSKAYRR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058209">
        <property type="entry name" value="TPR_BSK1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22904:SF523">
        <property type="entry name" value="STRESS-INDUCED-PHOSPHOPROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22904">
        <property type="entry name" value="TPR REPEAT CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25575">
        <property type="entry name" value="TPR_BSK1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="Serine/threonine-protein kinase BSK1-like TPR repeats" evidence="4">
        <location>
            <begin position="6"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="11"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ81516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="617" mass="68461" checksum="306FC02C59C4E673" modified="2010-06-15" version="1">MAPTEAQVEESDDLKQQGNTSFGKKEFSAAYALYTQAIHLNPTSPALYSNRSAALLSMNKLPLALNDANQAIKLDPTWSKAYRRKASVLEAQKELDKAKGVFEESLKVVLADLRASPAQKEKEEAEIKRSIEAIDKKIAERPSYHKHLIKDNETFVGQRVMREFEQRCAAGEPMGSWPPNGTCLRRIWIAEMQFQNALSNLLQYHRRQIPGAAPGVLGIYGRVQTLEELTSALLEDSRVLRLDGVAIEKLQLCYQLEQHQRNAVGPNLSPKETIAEYNRRLSQPPQNGPLINSYGASFSIGSPGPLAGWDLVRPALQLSVRSALMNGCLAGIVDPGNEEKEFRRAVELIEEAWKVWPNVDGATRGRTLEETFLRGVKILLAQSIARSYGDSPTSRNAQVKKLKELKAIGQWLVDSFNGKPKPPNANGVRDDADPWWNIYYAHYVAPVACGHAFIAYYHTQMGIDWLEGEEQKASLRKGAEEYAIAAGWMSPDDPDRIARIWTAMFSLFNSGEAYYKSDFAACLEMGENAKEWTMPFFGTRFGIEEGHIGQKAGMAAVGMDQPPIGGEDVLVITKIMRDVWATRVSKYGEKEEAVGGTRIWGALGRGIMADLEADGLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5GAW7</accession>
    <name>D5GAW7_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Exocyst complex component EXO84</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Exocyst complex component exo84</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">GSTUM_00005338001</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in the secretory pathway as part of the exocyst complex which tethers secretory vesicles to the sites of exocytosis. Plays a role in both the assembly of the exocyst and the polarization of this complex to specific sites of the plasma membrane for exocytosis. Also involved in assembly of the spliceosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the exocyst complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Secretory vesicle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EXO84 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430086">
        <property type="protein sequence ID" value="CAZ81660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837469.1">
        <property type="nucleotide sequence ID" value="XM_002837423.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GAW7"/>
    <dbReference type="FunCoup" id="D5GAW7">
        <property type="interactions" value="173"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GAW7"/>
    <dbReference type="GeneID" id="9187828"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005338001"/>
    <dbReference type="eggNOG" id="KOG2215">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012488_2_0_1"/>
    <dbReference type="InParanoid" id="D5GAW7"/>
    <dbReference type="OMA" id="AAWLPNR"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030133">
        <property type="term" value="C:transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000264">
        <property type="entry name" value="Potential exocyst complex component Exo84"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1220">
        <property type="entry name" value="Exo84p, C-terminal helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1210">
        <property type="entry name" value="Exo84p, N-terminal helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033961">
        <property type="entry name" value="Exo84"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032403">
        <property type="entry name" value="Exo84_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042561">
        <property type="entry name" value="Exo84_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042560">
        <property type="entry name" value="Exo84_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21426">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21426:SF12">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16528">
        <property type="entry name" value="Exo84_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25345">
        <property type="entry name" value="PH_EXO84"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08700">
        <property type="entry name" value="VPS51_Exo84_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst component Exo84 C-terminal" evidence="14">
        <location>
            <begin position="436"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="136"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="26"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="200"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00057052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00065378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00071741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF16528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAZ81660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="658" mass="74140" checksum="29B6784C0E0DF6E7" modified="2010-06-15" version="1">MDKDRSKGLSLRKKRSEGKRSAKPVIGPPRQVPMPPMPGAKLGPASGPPQVRVRDKQMVADLVKRRMSTRFPMPSEPGANIPAMPNLPSGPNMIAAGAGARLPVKQPERSDGVNLDLDTFRDPNFNPDAFVATVLANASDEEIRDYQRRLKDIRNRTSSDLQKNVFVNRTQFILISKEIDKLKSEMRVLRNLLNDLHNTTSSLKTDSENSSTTSDLKARKAANRSSVADLTALHMSHLQALWKQVEGAQKFLPSVPGRHIILESRHWVELNAATWKPRRIVHIVLLNDHLLVATVKKKRTDNGSSSSPQIKNVAERCWPLAEIEMVDLSPNDSQNAKRAQVLNAVNLRVGKESFVYQNEDLSKKSELLRQFKKSADELRKALRAKSEDNLRRRDSMTFLAGRDPRLLENGGLLNSLSAHGSTSALFTVEGQTRNLRWVEAQMDELDEKIAHRQFEEAVVGVEKLRGLGRALTSNNALVAELINFKVDERASRLATIITADLTENPAKKSAVKANVQWLVRLDFEDRAREAFLEAKSDVIKKRTRQVRFEGDVPLYISQVALVQFTLIKNTVEIYNSCFEYRLASALVRWAKERADEYMDIFERQLHAIDQDSNVYGDCIEITRLHSTMLVDCGLDFKELKDPRLRARKDDGAAGLELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>D5GB99</accession>
    <name>D5GB99_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00000533001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430089">
        <property type="protein sequence ID" value="CAZ81792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837601.1">
        <property type="nucleotide sequence ID" value="XM_002837555.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GB99"/>
    <dbReference type="STRING" id="656061.D5GB99"/>
    <dbReference type="GeneID" id="9181503"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000533001"/>
    <dbReference type="eggNOG" id="KOG1231">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_714089_0_0_1"/>
    <dbReference type="InParanoid" id="D5GB99"/>
    <dbReference type="OMA" id="RINEITY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.462.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050416">
        <property type="entry name" value="FAD-linked_Oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42973">
        <property type="entry name" value="BINDING OXIDOREDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G17690)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42973:SF54">
        <property type="entry name" value="FAD-BINDING PCMH-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072269" evidence="5">
        <location>
            <begin position="25"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="6">
        <location>
            <begin position="89"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ81792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="387" mass="41411" checksum="59E5B6BDF99B8EAE" modified="2010-06-15" version="1">MFLKPPLTTFFLLLLSQTFQPADSRNTESTVLRLSPAKIACLDSRITGWGYSYISTDPEFEYLRTINLYSPPPNLQGEILTNPLSDHSAWATPECILSPRSAAEVSSALKAITAAGAPFAVRSGGHSPNLGWANIEGGVLVELSRINEITYDPANTNVVVGTGSRWGGVYSALEAYGRTAVGARVSPVGVGGFLLGGGLSHVSNEYGFGCDNVVEYEVVLANGTIVNANADAHPELYWALKGGTSNFGTPPPLPQPPTHQLTNCPHAGIVTRFTLKTIPMSKIWGGVITYNATQAVAVMDAIHAYHAEHISDLKSAISVQFILNAGVVLVTLMYAENVGSSPAAFAPFEELPHVASSMGSKSFVEFHDEWLVGDAANRYVFREREGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBF6</accession>
    <name>D5GBF6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00000449001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430094">
        <property type="protein sequence ID" value="CAZ81849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837658.1">
        <property type="nucleotide sequence ID" value="XM_002837612.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBF6"/>
    <dbReference type="GeneID" id="9184423"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000449001"/>
    <dbReference type="HOGENOM" id="CLU_1143258_0_0_1"/>
    <dbReference type="InParanoid" id="D5GBF6"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ81849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="243" mass="26429" checksum="918C3E5340661F00" modified="2010-06-15" version="1">MEKNGELDSSFMAVDPVSPASNNSKDLLDEPAAPRRNSTDDNDPTRPRKRLAAMAIHDDMKDVEVAVESPREASSPLQDRASQLALGDRHSGEEEDRSNLGDEEGAVAPAAGGVGKSITIHLRSSVLHQSEENQSQDGYASDRVNGSTQSTIQKSEQLQPLDSPQATSPNGSIRTPDVEVEEVEEMDEGEDHLSSHITIVNNYLNNPVNTGGPDRVKDLMDSMVSKLERGKRCLNYDPAKLWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBG1</accession>
    <name>D5GBG1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="11">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00000458001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the glutamate 5-kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430094">
        <property type="protein sequence ID" value="CAZ81854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837663.1">
        <property type="nucleotide sequence ID" value="XM_002837617.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBG1"/>
    <dbReference type="FunCoup" id="D5GBG1">
        <property type="interactions" value="446"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GBG1"/>
    <dbReference type="GeneID" id="9184428"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000458001"/>
    <dbReference type="eggNOG" id="KOG1154">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025400_1_1_1"/>
    <dbReference type="InParanoid" id="D5GBG1"/>
    <dbReference type="OMA" id="NLAFPPH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004349">
        <property type="term" value="F:glutamate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901607">
        <property type="term" value="P:alpha-amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04242">
        <property type="entry name" value="AAK_G5K_ProB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21157">
        <property type="entry name" value="PUA_G5K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.130.10:FF:000008">
        <property type="entry name" value="Glutamate 5-kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1160.10:FF:000020">
        <property type="entry name" value="Glutamate 5-kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00456">
        <property type="entry name" value="ProB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041739">
        <property type="entry name" value="G5K_ProB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001057">
        <property type="entry name" value="Glu/AcGlu_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011529">
        <property type="entry name" value="Glu_5kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005715">
        <property type="entry name" value="Glu_5kinase/COase_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019797">
        <property type="entry name" value="Glutamate_5-kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01027">
        <property type="entry name" value="proB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43654">
        <property type="entry name" value="GLUTAMATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43654:SF3">
        <property type="entry name" value="GLUTAMATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000729">
        <property type="entry name" value="GK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00474">
        <property type="entry name" value="GLU5KINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00902">
        <property type="entry name" value="GLUTAMATE_5_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0641">Proline biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PUA" evidence="10">
        <location>
            <begin position="316"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ81854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="420" mass="45605" checksum="AC577D87F857E61A" modified="2010-06-15" version="1">MNLSSEFDRFGGYSTSMKGKRALTIVIKLGTSSICDERTHDPLLSNLSLLVETAVRLRRDGHRVILVSSGAIGIGLRRLDIEKRPKHLSAAQAIAAVGQCRLIALWDALFTQLQQPVAQVLLTRNDIADRTQYLNASNTFAELLSMGIIPIVNENDTLAVNEIKFGDNDTLSAITAGMVNADYLFLMTDVDCLYTDNPRTHPNAQPIEVVEDISTLKVDVSSTGSTLGTGGMATKLTAARLATSAGVTTIITRSSIPSNIFHIVAYIQSLKEADPNTQPQTPTIPPSHPLHTRFLAKPRALRDRYFWILHGLAPHGTVYIDEGAYGALTRRDKAGLLPVGVVDVAGTFAQQEAVNLAVVQRIVGGGYVIKGPEVGKALVNYSSTEIARIMGQQSSKIQELLGYAGMYFHLLSSPFILPRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBG9</accession>
    <name>D5GBG9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00000495001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430095">
        <property type="protein sequence ID" value="CAZ81862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837671.1">
        <property type="nucleotide sequence ID" value="XM_002837625.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBG9"/>
    <dbReference type="STRING" id="656061.D5GBG9"/>
    <dbReference type="GeneID" id="9184436"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000495001"/>
    <dbReference type="eggNOG" id="ENOG502SWS4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_866512_0_0_1"/>
    <dbReference type="InParanoid" id="D5GBG9"/>
    <dbReference type="OMA" id="AMMCKNN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00048">
        <property type="entry name" value="DSRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="entry name" value="Ribonuclease III domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00636">
        <property type="entry name" value="Ribonuclease_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="RNase III domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50142">
        <property type="entry name" value="RNASE_3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RNase III" evidence="2">
        <location>
            <begin position="16"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ81862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="321" mass="35057" checksum="491DDA47A4E647D1" modified="2010-06-15" version="1">MSAPAPWYAPIPIPQFNTPIARYDFSSKELEQLLYASVVHERSFASSPEQRDTFEALTWVGGSVLRGLTTVLLASMFPTSKKNVLNDIRQALEDKTFYAHLSRRLGLHNHISLTYPAGESDHILSGLFTAYVGAIQRELGVDGFPTISGTFCQLLRPYAEAYKSHHDKYIAANPHLPPTPIISPVSTAPSSSLAAQQQQKVYPILQTAATTDSGAAKLRRQHVSVYTSRLMEYAAKCKLPQPSFNFTDNGCQGAAIQWCATINMNGADIASAVASTKLEAKHLASKDALQIIIGSNVPWPVTVSGRRQKKLIARRSGGMLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBM9</accession>
    <name>D5GBM9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00005471001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430097">
        <property type="protein sequence ID" value="CAZ81879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837688.1">
        <property type="nucleotide sequence ID" value="XM_002837642.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBM9"/>
    <dbReference type="GeneID" id="9184453"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005471001"/>
    <dbReference type="eggNOG" id="KOG2922">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012349_5_1_1"/>
    <dbReference type="InParanoid" id="D5GBM9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3730.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008521">
        <property type="entry name" value="Mg_trans_NIPA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570:SF92">
        <property type="entry name" value="SPICHTHYIN, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05653">
        <property type="entry name" value="Mg_trans_NIPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="36"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="84"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="112"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="205"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="243"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="274"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="419"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="499"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="583"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="595"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="613"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="717"/>
            <end position="727"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ81879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="744" mass="79421" checksum="14F1734BACE30454" modified="2010-06-15" version="1">MSSAIASATASALASATSKAASGGHGLGGAGGRPPVYKAIGVALAVSSGVFIGVSFVLKKKGLLAANLKDGKEAGEGYGYLKNAWWWSGMILMILGEICNFCAYAFVEAILVTPLGALSVVITAILSSIFLGERLSFVGKIGCFMCIIGSIVIVINAPEQSSVNSIQDMKHFIISPGFLSYAGVVILGCIGVVVWVAPKYGNKSMMVYISICSLIGGLSVVATQGLGAAVVKQASGTPQFNQWFLYVLLVFVVVTLLVEIVYLNKALNIFNAALVTPTYYVCFTSSTIVTSAILFRGFKGTPSSITTVVMGFLQICSGVVLLQLSKSAKDVPDTEIFRGDLDQVRTVAEQSEPESEPKADAIRGTAAIIRRISIARQKAEVEEARRIREEKINEMLHGPNQNVEWDGVRRRVTFSAHGAPRRRNTMSGSHPPLGMSRMPDLEEGVESSTPAADVGPGRRRSMSVDEAMRAQVYGIQPNEPDQQPASIVERVKSLFIPRQRSSPSLRSEAGIATPHRDFRDSSAPPLAPSHTSSEHPPPLPMVDMSAPPGTPHLAVTPPVYEHSTSTRDFAPGQSSSSHRRTTSRGSEYTALAPQTSPPTPPPAPSTPDLRPSSGSSGARRQFSFQSMFGRHGHTHSRSNSSTVHVEEPVSYSAPHSQLHLPRLGLGSRGSSAPGAIKTEEEMVGLVKGDSRNNALPPYDSDEEKGKAPTASVRYVESDPEEEQETEREESLLKKWEAGGGPGRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBZ5</accession>
    <name>D5GBZ5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00005721001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430098">
        <property type="protein sequence ID" value="CAZ82038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837847.1">
        <property type="nucleotide sequence ID" value="XM_002837801.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5GBZ5">
        <property type="interactions" value="915"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GBZ5"/>
    <dbReference type="GeneID" id="9181307"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005721001"/>
    <dbReference type="eggNOG" id="KOG0920">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001832_4_0_1"/>
    <dbReference type="InParanoid" id="D5GBZ5"/>
    <dbReference type="OMA" id="LFRVCNM"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17917">
        <property type="entry name" value="DEXHc_RHA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23827">
        <property type="entry name" value="RWD_YLR419W-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001214">
        <property type="entry name" value="DExH-box ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056328">
        <property type="entry name" value="DSRM_DHX29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059023">
        <property type="entry name" value="RNA_hel_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF267">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE YLR419W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24385">
        <property type="entry name" value="DSRM_DHX29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26026">
        <property type="entry name" value="RNA_hel_CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05773">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50908">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0347">Helicase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RWD" evidence="4">
        <location>
            <begin position="419"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="5">
        <location>
            <begin position="622"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="545"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="58"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ82038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1303" mass="143941" checksum="9581A72EA49BDC9F" modified="2010-06-15" version="1">MPPKKKSSRSSVAESPEASPPPPSPAGSSAKKSKAKPGTNKASRTTVGSKGKAPVVTSSEAGSSASASKGGESRTLTVQQLIGGLSWTGKLPQTLFYEHCIKAGWNKPDYYVKDATKGFVSSVTVTYSNPKSSHTESMRYTPPFEMPDGTPINLQMASALEARHFAATYALHRVSSMKNIHMMLPPTHKSYWAIFDEIKRMEFAKNHAWMYVADPFLDKREWLAAEAAKAKALAEVKKEKPAEVVDEKMKGWYRVPVVDMGISQRREVEGLVRKYHVWNPVGVSMTRDLMSKVVSELVRLDFKRAHVEEACDWVKDKEEALEWLLIHVPEDDLPVRFLPGNYATGVTFSSGDLPMEYAAKRMAPHPQLSLALLTLFQDYLSLRAAEALMQLLVYGCQKTPQGTREESLISFSDTDLWTEEIGTLAAIWGPERFTQITPTHFKVLLSNPPIQGSKLPKKIFLEIRKPTSATWELEYPQTIPTFTLSTEDDGDDKLSAQVKLSIIKQTAKHASTLLGEQMIFTAVDWLESNIARIVQSPGRLCDVTSGVTGSNEDDVPPPNKASRQTFRHRNSRNSGAIDWTPGKEASMRLLATKKAKYSLPDMQNMLQVRMRLPAWDLKEEIIGAVNDCQVTIISGETGSGKSTQAVQFILDDMIDQHLGHITNIICTQPRRISALGLADRVSDERCSRVGQEVGYAIRGESKQNPATTRITFVTTGVLLRRLQMGDTLDEVTHIFVDEVHERSLDTDFLLILLKRKLAARKDLRVVLMSATLDAKIFSDYFGGEGKVRRVEIKGRTFPVEDYYLDQMLGSHDGGILIFLPGNPITFSHSILLLTDAGTMEIQRCIEAIKTLPCGIERFHALPLHASLTPNDQRKVFPQPPSGKRKVIAATNKLHTIPPVVSSNFSKHGPPAQHASNLYTRNAENKMAERPEPELKRVPLEQTCLAVKAMEDDVRGFLAGALTPPDTMAVEGAMRLLERMGAIADDDLTALGRHLVLIPADLRCSKLMVYGAIFGCLDVSVTIASILTTRSPFINTPATRDACQLARASFAHGQGDLLADCHAHQEWTRLRQTTSVRDVKRWCEENHLSHHTLLDISSNRAQYISSLKEIGFLPLSSSFSDFASLNQNQEYAVLHRALIAAAFNPQIARIALPEQRFAGSASGALAVDPEARTIKYYTHSSGRAFVHPSSTLFGATGFTGDARFLSYFSKMETNNKVYLKDVTPTNAYSLLLFGGALEVDTTGRGITVDGWLKLRGWGRIGILVARLRAMLDQVLQDKIDDPGLELAGNEVVACVARLVGSNGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>D5GBZ8</accession>
    <name>D5GBZ8_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Palmitoyltransferase</fullName>
            <ecNumber evidence="10">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">GSTUM_00005728001</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="10"/>
    <dbReference type="EMBL" id="FN430098">
        <property type="protein sequence ID" value="CAZ82041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002837850.1">
        <property type="nucleotide sequence ID" value="XM_002837804.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GBZ8"/>
    <dbReference type="FunCoup" id="D5GBZ8">
        <property type="interactions" value="420"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GBZ8"/>
    <dbReference type="GeneID" id="9181310"/>
    <dbReference type="KEGG" id="tml:GSTUM_00005728001"/>
    <dbReference type="eggNOG" id="KOG1315">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024136_0_1_1"/>
    <dbReference type="InParanoid" id="D5GBZ8"/>
    <dbReference type="OMA" id="GEFRFCK"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12246">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="169"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="214"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="12">
        <location>
            <begin position="122"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="361"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="361"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="382"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="400"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="449"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="467"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="484"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAZ82041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="493" mass="55453" checksum="4A775DF4ECBE8141" modified="2010-06-15" version="1">MDSPMLSPAMGSPRRKARFSCRRTDKLCCTLATYFPLLFVYSTLSWAVYTYSYSICWQNVGGMKGGVLSFMGFALCALANWSYTTAVFTNPGSPMDTDKHAYSHLPTTETAYHSSITVKSSGQERFCKKCECRKPDRTHHCSTCRKCVLKMDHHCPWLSNCLGLYNYKAFLLFLIYTSVFSLLCFVVSCIYVYQELFSTGEKKYSPEDLTPVNWVLLAVVAGVIGLVLSGFTIWHLTLVASGMTTIESLEKVRYNSPTLSRRCPPPPEDAHHLYDDPNYQARQENIEAFQRYNTYIMEEASNNLPHAFHLGRRENFQQVFGGKDQWMRWFIPVFSGIGDGWNWETSSEWKTAVEAMNKERERLAREQGERERRAGWGCEAPGSGGNWKGGNPNGLVKHATANSSAHLQQSKAERVLGRIPGSYSDHDMTGTGSGGERVPLRRMNKSSEEVDFSDDDDVDCNVDLEQGTHNTGTSWVSWGPGSAGTETNTGRWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>D5GC91</accession>
    <name>D5GC91_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00000579001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430106">
        <property type="protein sequence ID" value="CAZ82134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GC91"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000579001"/>
    <dbReference type="HOGENOM" id="CLU_2160246_0_0_1"/>
    <dbReference type="InParanoid" id="D5GC91"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="24"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="111" mass="12047" checksum="5FC0E757B42CA04A" modified="2010-06-15" version="1">MRIPLLTSLSPKSVRTRFGKRSRTSSTSSTSTTSSSASAAAGKPKLQRKPSGLSLYELYSGSRGGSTGMVASSSVGYWSYEQEQERKEEEGLSVLEPRPRHCVGLFEVLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCK0</accession>
    <name>D5GCK0_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00000665001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430111">
        <property type="protein sequence ID" value="CAZ82243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838052.1">
        <property type="nucleotide sequence ID" value="XM_002838006.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCK0"/>
    <dbReference type="STRING" id="656061.D5GCK0"/>
    <dbReference type="GeneID" id="9186429"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000665001"/>
    <dbReference type="HOGENOM" id="CLU_092869_3_0_1"/>
    <dbReference type="InParanoid" id="D5GCK0"/>
    <dbReference type="OMA" id="VWTTEVV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005199">
        <property type="term" value="F:structural constituent of cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038843">
        <property type="entry name" value="Sed1/Spi1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35523">
        <property type="entry name" value="CELL WALL PROTEIN SED1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35523:SF1">
        <property type="entry name" value="CELL WALL PROTEIN SED1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="104"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="143" mass="14799" checksum="D1C1B81CFEDB0007" modified="2010-06-15" version="1">MVLNKLPLRFHQNYCVYCPLPTTIVENNKTYTVTKATTLTITNCPCTRTKVYTTILPTTICPGKCSSVPVGTGPTHYAPTASYTPPVYPNGTIPTSKPSAPGNSTKPAPTSAPTAPPFTGSANKATVAGAALAGLIGAVAYIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCK9</accession>
    <name>D5GCK9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00000706001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430114">
        <property type="protein sequence ID" value="CAZ82252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838061.1">
        <property type="nucleotide sequence ID" value="XM_002838015.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCK9"/>
    <dbReference type="STRING" id="656061.D5GCK9"/>
    <dbReference type="GeneID" id="9187887"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000706001"/>
    <dbReference type="eggNOG" id="KOG4324">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009486_1_1_1"/>
    <dbReference type="InParanoid" id="D5GCK9"/>
    <dbReference type="OMA" id="CCEFTGY"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051286">
        <property type="term" value="C:cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070319">
        <property type="term" value="C:Golgi to plasma membrane transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21044">
        <property type="entry name" value="Rab11BD_RAB3IP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040351">
        <property type="entry name" value="RAB3IL/RAB3IP/Sec2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009449">
        <property type="entry name" value="Sec2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14430">
        <property type="entry name" value="RABIN3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14430:SF0">
        <property type="entry name" value="SEC2P DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25555">
        <property type="entry name" value="RAB3A-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06428">
        <property type="entry name" value="Sec2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144284">
        <property type="entry name" value="Sec2 N-terminal region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GDP/GTP exchange factor Sec2 N-terminal" evidence="3">
        <location>
            <begin position="58"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="517"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="23"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="210"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="602"/>
            <end position="618"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ82252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="686" mass="74177" checksum="53F240AD2BC98E3C" modified="2010-06-15" version="1">MTADRPNLCKSASESALRVPTPDGTSSGRSTPGENGSMDLNVEVAALSNKLINAINHQTQLDDSLSQTRHELEISRARVKQLEAVARENTDMLAKGLLVKKSDIEAETKQLKDRLNEERRLRTKAEEEKRVIERELEGLTAALFGEANEMVSVARQKEEAVDKKNEQLRSQLADTQLLLASHQEQLAELKQVIQQMTAEKEEAETTMSTGTPSTPALGSRTSKESLGRVFEALHLSPDLATIEDVQPCPPTSLTSLIYPVLRHDIPAYLDFCEVLHSPKGGTKTSPIPRLSSGSFSPIQAMGIGMGISYGSSSTSNSSTGLSSALFGARVDKGSDGRPASPAGVGSAINGNAQQQIGLALKETKFYKRVLVEDIEPTLRVDLAPGLSWLARRNVSSAIIDGSLQIDPLPMNSKSSAVSCSMCGESRLDEQHVRTHQMRTSDSATALRHMLCTYCVNRTRSVCDFLAFLRTLKEGMWKCDSEGDEKHAWEECIKLRERMFWCRVGGGVIPAFIHRESARNSEDHGGAGSLRPAGDETPRDLQSPRTPELRGCEGQVKLEVPRRRISRELPPIVPGKASVSETPSPIAPSPVDKELPPPPRISLDSTANEEATIENQEPSWENGPAVGMTSEHSPPAVSKQPIEPESLAAPIAKTPGPPATTTKKEPTAPAGAAGSDMEIPQAVPGAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCN2</accession>
    <name>D5GCN2_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="9">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00000743001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peroxiredoxin family. Prx5 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="FN430118">
        <property type="protein sequence ID" value="CAZ82275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838084.1">
        <property type="nucleotide sequence ID" value="XM_002838038.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCN2"/>
    <dbReference type="FunCoup" id="D5GCN2">
        <property type="interactions" value="527"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GCN2"/>
    <dbReference type="PeroxiBase" id="11114">
        <property type="entry name" value="TmelPrxV"/>
    </dbReference>
    <dbReference type="GeneID" id="9187898"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000743001"/>
    <dbReference type="eggNOG" id="KOG0541">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072440_3_0_1"/>
    <dbReference type="InParanoid" id="D5GCN2"/>
    <dbReference type="OMA" id="SAWGKQH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03013">
        <property type="entry name" value="PRX5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037944">
        <property type="entry name" value="PRX5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013740">
        <property type="entry name" value="Redoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10430">
        <property type="entry name" value="PEROXIREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10430:SF39">
        <property type="entry name" value="PEROXISOMAL MEMBRANE ASSOCIATED PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08534">
        <property type="entry name" value="Redoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 7" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="5 7" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Redoxin" evidence="8">
        <location>
            <begin position="37"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate" evidence="6">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR637944-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="CAZ82275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="189" mass="20616" checksum="6C6BDD6C802F911C" modified="2010-06-15" version="1">MFSLLRPWTIAFPSTHRRLSTASIHGFRASLGAFIEVGDLVPNIQLRENTPGKRVSIAEELKGKKKALILGVPGAFSPACSAAHIAKYIEAKFDVPTYVVAINDPFVTKAWKDSLTSRDDENFRFLADPSSEFTDAVGMKFDATPIFGGPRSKRYALIVEDGKVSKVFAEPDNTGVSVTSADNILPELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCT9</accession>
    <name>D5GCT9_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00000825001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430121">
        <property type="protein sequence ID" value="CAZ82332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838141.1">
        <property type="nucleotide sequence ID" value="XM_002838095.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCT9"/>
    <dbReference type="STRING" id="656061.D5GCT9"/>
    <dbReference type="GeneID" id="9182436"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000825001"/>
    <dbReference type="eggNOG" id="ENOG502S0ES">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_432922_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCT9"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047092">
        <property type="entry name" value="AFUB_07903/YDR124W-like_hel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021264">
        <property type="entry name" value="AFUB_079030/YDR124W-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36102">
        <property type="entry name" value="CHROMOSOME 10, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36102:SF1">
        <property type="entry name" value="YDR124W-LIKE HELICAL BUNDLE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11001">
        <property type="entry name" value="AFUB_07903_YDR124W_hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Subtelomeric hrmA-associated cluster protein AFUB-079030/YDR124W-like helical bundle" evidence="2">
        <location>
            <begin position="161"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="203"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="438"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="472"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="508"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ82332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="632" mass="69322" checksum="9DD04753CEEF4104" modified="2010-06-15" version="1">MKKGIQRHHRNDYDKDHQSPNRGSNGVDIDGDMEDEDDGNEGDIDGGQWADAEGDEDNHDSSSIHQQSTPPEDSDSLSSPKAMRANGGSGMLLPPPPADQRRIIRANGANRKRPVQPELQTTPAKKRRSTVKRSVRRADSSSGESNDVVLHEEIEYKGLDITNEGEVNTFFETRLRQMQQLVCKVVAKCWIKVIEPKKQSNFPYNRGEESKPSWWPPNARHKEPDHLMKPERLRLLLTMLRCRKVPVAKLEAATAEATAHIPPEKAPLLEEIYRVAKMEERYRSGGLAPGTLCFVAASEKIVKSPNQKVPSPKVNGDSSSPIIASSGALRDMGRRMSSAQSPGQPQLSPIIANGESGLRAAPRLNTDIVTGSIPHIQPQNPFRGNRGQSNVQPLNTQAGLGYSTNGYVSQTSPVPDEQYMDQNQLPLSYTHHYLVNPQQQQPAPHRNSIATSHGYPGANPPPAHSSLRRAHTSPNISQQSQSYAGWPPQMSNTPLVNGMYSPYSPNTQPQHSPHGSHSGHPSHGPQSASNPPTPVTATSYQQLPHPLPPMHQHQQSQLVTSPSHDIPGGDERGEQQFETQAMAGSIRSGSLQHQHHIGHVSYSDFLENEHDLGAVQENAVSVEDGQQGGRSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>D5GCU5</accession>
    <name>D5GCU5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00000752001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430123">
        <property type="protein sequence ID" value="CAZ82338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GCU5"/>
    <dbReference type="STRING" id="656061.D5GCU5"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000752001"/>
    <dbReference type="HOGENOM" id="CLU_934439_0_0_1"/>
    <dbReference type="InParanoid" id="D5GCU5"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019327">
        <property type="entry name" value="WKF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22306">
        <property type="entry name" value="CHROMOSOME 7 OPEN READING FRAME 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22306:SF2">
        <property type="entry name" value="CHROMOSOME 7 OPEN READING FRAME 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10180">
        <property type="entry name" value="WKF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WKF" evidence="2">
        <location>
            <begin position="174"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="268"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="268"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ82338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="298" mass="32674" checksum="782278403C27060F" modified="2010-06-15" version="1">MPDDQSDGRKRNARSSPDEENSEKDKKRKKATSLEDVKEQEKKCTTSPSPPPQPEKHHTTPPSIKTVTTPKSLLKKSNNKTPTPRTKTVTFADDAKSTDGDSIANTIRSPGGTGGRSNIPRLPVSPQPHPALLEQQSFSPAATTATTQQGVLSHSPNLSEDHSNQQQNTKPQIAYLLNYYQARSSWKFSKTKQNWIIRNIWNLSEFSAEVLQSALWAYVNGLAAQGPKDRLLTEAKEVAKTRREELGAVDAEDDPKYRRAKLVLTALGDDDIPSEDEGGDADGTDGDNKENVCAEGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDC6</accession>
    <name>D5GDC6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="8">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">GSTUM_00006149001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 32 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430142">
        <property type="protein sequence ID" value="CAZ82519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838328.1">
        <property type="nucleotide sequence ID" value="XM_002838282.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDC6"/>
    <dbReference type="FunCoup" id="D5GDC6">
        <property type="interactions" value="33"/>
    </dbReference>
    <dbReference type="GeneID" id="9183983"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006149001"/>
    <dbReference type="eggNOG" id="ENOG502QS3D">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036369_3_0_1"/>
    <dbReference type="InParanoid" id="D5GDC6"/>
    <dbReference type="OMA" id="NRHSWSF"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000030">
        <property type="term" value="F:mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051999">
        <property type="term" value="P:mannosyl-inositol phosphorylceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.20:FF:000001">
        <property type="entry name" value="MIPC synthase subunit (SurA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051706">
        <property type="entry name" value="Glycosyltransferase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007577">
        <property type="entry name" value="GlycoTrfase_DXD_sugar-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32385">
        <property type="entry name" value="MANNOSYL PHOSPHORYLINOSITOL CERAMIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32385:SF20">
        <property type="entry name" value="MANNOSYL PHOSPHORYLINOSITOL CERAMIDE SYNTHASE CSH1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04488">
        <property type="entry name" value="Gly_transf_sug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="270"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="CAZ82519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="330" mass="38880" checksum="8AA1DE85C1B80D7B" modified="2010-06-15" version="1">MRKGLILFLVCNLIIIGFLVNFLFTLITLLFEDCSADAIHSVDIPAPNSQLIDSRPQLIPKIIHQTWQNETVPEKWREAQQSCLDLHGDYEYKLWTNEKARDFVAKEYPWFIGTYDSYPYPIMRADAIRYFVLAHFGGIYIDLDDGCNRRLDPLLSYRAWVRKTIPTGISNDAMGAVPQHPFFLRVIESLQTYNRNWHVPYITVMYGTGPLFLSVIWKEYIRWGTTEEGRVRVLMPEEYNKNPWSFFAVYKGSSWHSSDAKTIFWMGQHWLFLTVCGFSLAITLYLTGFWIYKRLHLRNTNYTEQPSPWWWPRRETVVGAGFKRLPKHEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDG4</accession>
    <name>D5GDG4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="3">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">GSTUM_00000999001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430143">
        <property type="protein sequence ID" value="CAZ82556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838365.1">
        <property type="nucleotide sequence ID" value="XM_002838319.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDG4"/>
    <dbReference type="GeneID" id="9184015"/>
    <dbReference type="KEGG" id="tml:GSTUM_00000999001"/>
    <dbReference type="HOGENOM" id="CLU_2265675_0_0_1"/>
    <dbReference type="InParanoid" id="D5GDG4"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="23"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="CAZ82556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="103" mass="11318" checksum="48FE8C6BCFEC1603" modified="2010-06-15" version="1">MGLEEVCSPSDGSDDLSVPPSPRRSTSSLPDPATAAQPLPAAEKFHPAVYVVVWIILSSAVIIFNKWVLFNKKFLCHSNIPYNMAFGVCNDRYPDPRTDDTPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDL5</accession>
    <name>D5GDL5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00001117001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430149">
        <property type="protein sequence ID" value="CAZ82608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838417.1">
        <property type="nucleotide sequence ID" value="XM_002838371.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDL5"/>
    <dbReference type="FunCoup" id="D5GDL5">
        <property type="interactions" value="18"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GDL5"/>
    <dbReference type="GeneID" id="9181614"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001117001"/>
    <dbReference type="eggNOG" id="KOG2919">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022731_0_1_1"/>
    <dbReference type="InParanoid" id="D5GDL5"/>
    <dbReference type="OMA" id="IRTWILP"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051150">
        <property type="entry name" value="SWT21/TCAB1_mRNA_Telomere"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13211">
        <property type="entry name" value="TELOMERASE CAJAL BODY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13211:SF0">
        <property type="entry name" value="TELOMERASE CAJAL BODY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ82608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="391" mass="41860" checksum="606E7264983B3C13" modified="2010-06-15" version="1">MSLLKPPKLVASTGTTYNCTVAETSARELSVQKWSGLGNPHESNLFKSAQWSPDGTCVLSNSEDNILRTFVLPVDLLDPSDAPKSLTPYSQTASAEPVSCLAIYPSMSLADPATCCFLKSTRDHPIHLHDLLVSNTRASYPLINPQTEKYLSPQSMLFTPKDANKFIAGATSQISFFDLQRPGEGPYLDLKTIPTRRSVQTGETMKGVVSALAIDNGVLAAGTLSRQVGLYDDCGSGSTIGVFTVDGEGGGITQLLWSKCGRYLHVVERKSDVISVYDVRVAGEKVESLEGRMAQTNQRIGVDIAYSLEGEVVSGGIGGMVSVWGTGGKGEKGFEWKAHDDVVSGVAIHPGGVVMATCSGTRKLSSLGEDSDDDESSEERKWDNGLMIWAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>D5GDZ4</accession>
    <name>D5GDZ4_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cytochrome c oxidase subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">GSTUM_00001112001</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>4 Fe(II)-[cytochrome c] + O2 + 8 H(+)(in) = 4 Fe(III)-[cytochrome c] + 2 H2O + 4 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:11436"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="7.1.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:11437"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="11">Binds a copper A center.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Mitochondrion inner membrane</location>
            <topology evidence="11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the cytochrome c oxidase subunit 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430155">
        <property type="protein sequence ID" value="CAZ82737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838546.1">
        <property type="nucleotide sequence ID" value="XM_002838500.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GDZ4"/>
    <dbReference type="FunCoup" id="D5GDZ4">
        <property type="interactions" value="190"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GDZ4"/>
    <dbReference type="GeneID" id="9184532"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001112001"/>
    <dbReference type="eggNOG" id="KOG4767">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036876_2_3_1"/>
    <dbReference type="InParanoid" id="D5GDZ4"/>
    <dbReference type="OMA" id="WSYEYTD"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045187">
        <property type="entry name" value="CcO_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002429">
        <property type="entry name" value="CcO_II-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011759">
        <property type="entry name" value="Cyt_c_oxidase_su2_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036257">
        <property type="entry name" value="Cyt_c_oxidase_su2_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027434">
        <property type="entry name" value="Homing_endonucl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22888:SF9">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22888">
        <property type="entry name" value="CYTOCHROME C OXIDASE, SUBUNIT II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00116">
        <property type="entry name" value="COX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02790">
        <property type="entry name" value="COX2_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01166">
        <property type="entry name" value="CYCOXIDASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81464">
        <property type="entry name" value="Cytochrome c oxidase subunit II-like, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55608">
        <property type="entry name" value="Homing endonucleases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50857">
        <property type="entry name" value="COX2_CUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50999">
        <property type="entry name" value="COX2_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0186">Copper</keyword>
    <keyword evidence="7 11" id="KW-0249">Electron transport</keyword>
    <keyword evidence="9 11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome oxidase subunit II transmembrane region profile" evidence="14">
        <location>
            <begin position="15"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome oxidase subunit II copper A binding" evidence="13">
        <location>
            <begin position="113"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="CAZ82737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="206" mass="23709" checksum="D47509CFF0A3107B" modified="2010-06-15" version="1">MFQLLLNYIHEYCDAPRPWGLYFQDSASPQMEGLVELHDNIMFYLIIILFGVGWVLVSVITNYNSVKSPISHKYLTHGTLIELFADINNIIIPLFNKYPLIGVKKLNFQDFCIIAELMKNKAHLTTKGYQYSDFLNDDNENIEFDSYMIPEEDLEDGGLRMLEVDNRVILPELTHVRFIITSADVIHSFASPALAIKCDAYPGFNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>D5GE77</accession>
    <name>D5GE77_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 18">3-isopropylmalate dehydratase</fullName>
            <ecNumber evidence="6 18">4.2.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16 18">Alpha-IPM isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17 18">Isopropylmalate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">GSTUM_00006413001</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 18">Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 18">
            <text>(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate</text>
            <dbReference type="Rhea" id="RHEA:32287"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:35121"/>
            <dbReference type="EC" id="4.2.1.33"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 18">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 18">Belongs to the aconitase/IPM isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.33" evidence="6 18"/>
    <dbReference type="EMBL" id="FN430164">
        <property type="protein sequence ID" value="CAZ82820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838629.1">
        <property type="nucleotide sequence ID" value="XM_002838583.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GE77"/>
    <dbReference type="FunCoup" id="D5GE77">
        <property type="interactions" value="207"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GE77"/>
    <dbReference type="GeneID" id="9185476"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006413001"/>
    <dbReference type="eggNOG" id="KOG0454">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006714_0_1_1"/>
    <dbReference type="InParanoid" id="D5GE77"/>
    <dbReference type="OMA" id="EDNEPHT"/>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00071"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009316">
        <property type="term" value="C:3-isopropylmalate dehydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003861">
        <property type="term" value="F:3-isopropylmalate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01583">
        <property type="entry name" value="IPMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01577">
        <property type="entry name" value="IPMI_Swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000007">
        <property type="entry name" value="3-isopropylmalate dehydratase large subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.19.10:FF:000003">
        <property type="entry name" value="3-isopropylmalate dehydratase small subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="entry name" value="Aconitase, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="entry name" value="Aconitase, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01026">
        <property type="entry name" value="LeuC_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01031">
        <property type="entry name" value="LeuD_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004430">
        <property type="entry name" value="3-IsopropMal_deHydase_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004431">
        <property type="entry name" value="3-IsopropMal_deHydase_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012235">
        <property type="entry name" value="3-IsopropMal_deHydtase_ssu/lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050067">
        <property type="entry name" value="IPM_dehydratase_rel_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033941">
        <property type="entry name" value="IPMI_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033940">
        <property type="entry name" value="IPMI_Swivel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00170">
        <property type="entry name" value="leuC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00171">
        <property type="entry name" value="leuD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002458">
        <property type="entry name" value="PRK01641.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004016">
        <property type="entry name" value="PRK05478.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009116">
        <property type="entry name" value="PRK12466.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43822:SF9">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43822">
        <property type="entry name" value="HOMOACONITASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001418">
        <property type="entry name" value="ACN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="Aconitase iron-sulfur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="10 18" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="15 18" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0408">Iron</keyword>
    <keyword evidence="13" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8 18" id="KW-0432">Leucine biosynthesis</keyword>
    <keyword evidence="14 18" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha" evidence="19">
        <location>
            <begin position="14"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase A/isopropylmalate dehydratase small subunit swivel" evidence="20">
        <location>
            <begin position="541"/>
            <end position="662"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00014371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00031631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00033368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRNR" id="PIRNR001418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="CAZ82820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="778" mass="84352" checksum="BE151EAF98E1FA3C" modified="2010-06-15" version="1">MPSVQEKGPTTLYDKIYAHHLVDGQTIYIDRHLVHEVTSPQAFEGLRNAGRIVRRPDCTLATVDHNIPTSSRANYKNAKTFIEEEDSRIQCLTLEDNVKAFGLTYFGLGDSRQGIVHIVGPEQGFTLPGSTVVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLINKKSKNMKIEVNGALLPGVTSKDIILHIIGKIGTAGGTGAVIEFTGSTIRSLSMEARMSMCNMSIEGGARAGLIAPDETTFEYLKGKPLAPKYESPEWNSALKYWRTLKSDPDAVWDIEVYVDARDVVPTVSWGTSPQDVVPITGKVPDPSEFGDSVARQRVVRALEYMGLAPNTPMQEITVDKVFIGSCTNSRIEDLRAAAKVVKGRRIAPNIMRAMVVPGSGLIKKQAEREGLDKVFLDAGFEWREAGCSMCLGMNPDILSPQERCASTSNRNFEGRQGAGGRTHLMSPAMAASAAISGKLADVRGFTFGAAEPEGAGGVPKTIIEAEHPDPVTDEDMDRIDDIPIDEEASNADAAAREDTSSASAVGVGIPKFLTLKGIAAPLPMANIDTDAIIPKQFLKTIKRTGLGSALFFALRFNPDGTENPNFVLNKEPYRNTKILVVTGPNFGCGSSREHAPWALLDFGIKCIIAPSFADIFFNNTFKNGMLPIAIDDSAALDKIARVAEMGKEVEVDLPNQVIKDGDGNELAKFDVEEFPKHCLVNGLDDIGLTLLIDDKIKEFEKKRTLEYPWLDGSGYLARRKRGNGPVKITAAKVPTTNRGFEKEITEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEA1</accession>
    <name>D5GEA1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Transcription initiation factor IIA subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00001216001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">TFIIA is a heterodimer composed of the large TOA1 and the small TOA2 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TFIIA subunit 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430169">
        <property type="protein sequence ID" value="CAZ82844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838653.1">
        <property type="nucleotide sequence ID" value="XM_002838607.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEA1"/>
    <dbReference type="FunCoup" id="D5GEA1">
        <property type="interactions" value="448"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GEA1"/>
    <dbReference type="GeneID" id="9185499"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001216001"/>
    <dbReference type="eggNOG" id="KOG3463">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112964_3_1_1"/>
    <dbReference type="InParanoid" id="D5GEA1"/>
    <dbReference type="OMA" id="MTTFDKC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005672">
        <property type="term" value="C:transcription factor TFIIA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10014">
        <property type="entry name" value="TFIIA_gamma_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10145">
        <property type="entry name" value="TFIIA_gamma_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.190:FF:000001">
        <property type="entry name" value="Transcription initiation factor IIA subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.18.10:FF:000003">
        <property type="entry name" value="Transcription initiation factor IIA subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.18.10">
        <property type="entry name" value="Transcription factor IIA (TFIIA), beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.190">
        <property type="entry name" value="Transcription factor IIA gamma subunit, alpha-helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009083">
        <property type="entry name" value="TFIIA_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009088">
        <property type="entry name" value="TFIIA_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003194">
        <property type="entry name" value="TFIIA_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015871">
        <property type="entry name" value="TFIIA_gsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015872">
        <property type="entry name" value="TFIIA_gsu_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10966">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIA SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02751">
        <property type="entry name" value="TFIIA_gamma_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02268">
        <property type="entry name" value="TFIIA_gamma_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009415">
        <property type="entry name" value="Hum_TFIIA_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47396">
        <property type="entry name" value="Transcription factor IIA (TFIIA), alpha-helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50784">
        <property type="entry name" value="Transcription factor IIA (TFIIA), beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription initiation factor IIA gamma subunit N-terminal" evidence="10">
        <location>
            <begin position="7"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription initiation factor IIA gamma subunit C-terminal" evidence="11">
        <location>
            <begin position="64"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00063181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR009415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ82844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="114" mass="13047" checksum="FF0FF5B61A22DD05" modified="2010-06-15" version="1">MSDQTYYYELYRRSCIGVALMDSLDDMISKGSITPQLALKVLSNFDRSIAETLANRVKANFSFKGHLDTYRFCDDVWTFLVTDVTFKFPQKNKPTISTNKIKIVSCNSKKPGEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEH7</accession>
    <name>D5GEH7_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="5">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">GSTUM_00006488001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430175">
        <property type="protein sequence ID" value="CAZ82920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838729.1">
        <property type="nucleotide sequence ID" value="XM_002838683.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEH7"/>
    <dbReference type="STRING" id="656061.D5GEH7"/>
    <dbReference type="GeneID" id="9181551"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006488001"/>
    <dbReference type="eggNOG" id="KOG0772">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014033_2_0_1"/>
    <dbReference type="InParanoid" id="D5GEH7"/>
    <dbReference type="OMA" id="KGDQYIT"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051858">
        <property type="entry name" value="WD_repeat_GAD-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16017">
        <property type="entry name" value="GASTRULATION DEFECTIVE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16017:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="92"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="196"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="286"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="357"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="529"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="44"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="72"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="529"/>
            <end position="539"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="CAZ82920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="557" mass="61335" checksum="553FE31072F404AF" modified="2010-06-15" version="1">MSLKPIPGEEEYAGSDNEPTIEEPGLAPDDPMRAFLPKSFGKQTMERDVKARVEQTRRAAPPPPKAKPPQDSDSDSDDSEEEEYPLSHELTIPAHTKPISSITLDPSGTRFVTSSHDTTIKFFDFHAMSTTHLHSFKTLEPSEAHHIHSVAFSPLDNGQHLLVVPAAPQAKILTRDGDTALEFVKGDPYLRDMHNTKGHVSEITGGCWSPVQRGVVVTAGTDSTVRIWDVQSRRGHRDVMVHKSRGKGGRSRICAVACIWNAGGPYSRPIMEVRDAHAKETWTGGLTFSSDGRLIATMGEDQYIKLWDTRKLKTPITTRSNFPTPTPTPIIFSPTNNTTLLTGDTSNTLHILSSATLTSEQTHPLSTPTPTPTTPSPLTSITWHSHINQILTGSGHGHLHILYSPTISRKGATTLLARAPRKRHIDDYNNSSITTTDLTTISGDSVILPNALLGKKGGVMDKRRPVMPAQTPWGKSQPDQAHIERSIGLSSMRDEDPREALLKYADVAEREPVFTAAWRETQPRTIYAEVEEEEGEDGEQDRVRGKVGAGTNKRVRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEI4</accession>
    <name>D5GEI4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="12">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">GSTUM_00006503001</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430175">
        <property type="protein sequence ID" value="CAZ82927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838736.1">
        <property type="nucleotide sequence ID" value="XM_002838690.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEI4"/>
    <dbReference type="STRING" id="656061.D5GEI4"/>
    <dbReference type="GeneID" id="9181558"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006503001"/>
    <dbReference type="eggNOG" id="KOG1015">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001161_1_0_1"/>
    <dbReference type="InParanoid" id="D5GEI4"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18007">
        <property type="entry name" value="DEXHc_ATRX-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044574">
        <property type="entry name" value="ARIP4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45797:SF1">
        <property type="entry name" value="HELICASE ARIP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45797">
        <property type="entry name" value="RAD54-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="502"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="916"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="77"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="221"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="392"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="28"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="87"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="117"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="168"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="394"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="CAZ82927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1100" mass="123910" checksum="DD1D93C3E98DE3F9" modified="2010-06-15" version="1">MERKTPPERPPETTPKGNKSLAEKSDDVGEDCGGDENDSDDESLGSGDGEPDYESEDEDLDGFIVSDDVRDYAGGRAIQVEGEDEVNEHLRQQEEGRLEALNELVDSSSEGEAPIPARKRRERGRRVVQDDEPDEEVEAPILAHGRSGFSDAELQGVRDGPGDKHSVTSRSGSGSSLSPVPKDKCKGGIRSPTPTVKQEPLLSQQIPNEKGPIPIIDLTVEDPPPGSKKQSRAPTMPTQRKNVEVEIQELSDDEETPLALLTRLIKEIEPQTRAALFYRMEENKIGKIFGLVISVARQIHVEKLKYYTGLDRYNSEIYRILTRLYLSWLLGKELSKHPKPSPEEFKKAETAAHFKEFRTILRKLTHVDDVPIVGGGGVSDYDQLGNGGRINIVGSRGSASGASSSTQSPVPQPTSSQKTKVRKPVEEDPHARALRERALKNQRNLMNRIRSQKRSSIAPDPEKVAINLGKYKRYNDIYFHPDFAGTLKPHQVDGVRFLWQQLVQSGEGRGALLAHTMGLGKTLQVITFLYTLATAAASKQEATFGQIPEALRESKTLILSPPGLVENWWDEFQKWLPQSPHERDQLDLSAIGQIYRADAIVSLTTRLSTITKWAKEGGVLLMGYTSFRMEISKAMNLNNHANYQHKQISDYLLNSPNIIIADEAHSLKNPKAQISQATRLFRSKSRVAMTGSPLSNNLMEYWTMIDWIDPGFLGPSKEFEAKFLHPIEDGLYADSTLSQRRNCLKMLTVLKKDIGPKVHRADVRVIEKDLPQKTEFLVKVPLTPWQVEMYTKFVTDPEVTGAIEGSDGKAKSGTRFFDIVHLLSLICNHPMCFVDTIEERGQKAQAAVRKNRQSKQDIFSLEDEELQELIDLDDLSPNEELLEGASKKYAWAKEMASKTQNPRALFHSYKMQLLKSIVEHSIRLGDKILIFSHGVYTLNYLDKLLEDWKIDFLRLDGKTRMSTRQSATKSFNSGRSDVFLVSTEAGGLGLNLPGANRIIIFDFKWSPMWEEQAVGRAYRMGQKKHVFVYRFHAVGTFEDLKRNKILFKSQLQSRVVDKQDPLRTAHKDVRQYLLEPANIPKEDLTEYRGKDPVLDKIMKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEP5</accession>
    <name>D5GEP5_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00001306001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430181">
        <property type="protein sequence ID" value="CAZ82988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838797.1">
        <property type="nucleotide sequence ID" value="XM_002838751.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEP5"/>
    <dbReference type="STRING" id="656061.D5GEP5"/>
    <dbReference type="GeneID" id="9184899"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001306001"/>
    <dbReference type="eggNOG" id="KOG0562">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1587690_0_0_1"/>
    <dbReference type="InParanoid" id="D5GEP5"/>
    <dbReference type="OMA" id="PRLEHHE"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033699">
        <property type="term" value="F:DNA 5'-adenosine monophosphate hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990165">
        <property type="term" value="F:single-strand break-containing DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000012">
        <property type="term" value="P:single strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019808">
        <property type="entry name" value="Histidine_triad_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032566">
        <property type="entry name" value="Znf-C2HE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12486:SF4">
        <property type="entry name" value="APRATAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12486">
        <property type="entry name" value="APRATAXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11969">
        <property type="entry name" value="DcpS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16278">
        <property type="entry name" value="zf-C2HE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00892">
        <property type="entry name" value="HIT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aprataxin C2HE/C2H2/C2HC zinc finger" evidence="1">
        <location>
            <begin position="91"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ82988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="168" mass="18697" checksum="8378D05EE216184B" modified="2010-06-15" version="1">MLHPFEAFKDEAFLARVRTDAEELKGLVARELRRKICGGGTAAGGKGVDWGAEVRVGVHAGPSMNHLHVHVISREGFSDRVKKRAHYNSFNTAFFVPLEELPLRDDDPRLEHHERARLLKMDYTCWRCGRGFENSFVGIKQHVAEEFVEWKKALIEAGGGGGGKATSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>D5GEW6</accession>
    <name>D5GEW6_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00006619001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430197">
        <property type="protein sequence ID" value="CAZ83059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002838868.1">
        <property type="nucleotide sequence ID" value="XM_002838822.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GEW6"/>
    <dbReference type="GeneID" id="9188597"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006619001"/>
    <dbReference type="HOGENOM" id="CLU_152050_1_0_1"/>
    <dbReference type="InParanoid" id="D5GEW6"/>
    <dbReference type="OMA" id="CAAMACC"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="98" mass="10479" checksum="AFFB19ADCAD59FCF" modified="2010-06-15" version="1">MTHGIDTKSTSPPPQYPQPTFQPPPPNQEYYPPPQGAYNQQPPPPQQYNQPYPPPQQPMVYQMPPGNGKVSSGGTATGGILAGLCAAMACCCCLDCIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="90" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFI1</accession>
    <name>D5GFI1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="13 15">DNA replication licensing factor MCM4</fullName>
            <ecNumber evidence="3 15">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">GSTUM_00006910001</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">Mel28</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Component of the MCM2-7 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the MCM family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3 15"/>
    <dbReference type="EMBL" id="FN430208">
        <property type="protein sequence ID" value="CAZ83274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839083.1">
        <property type="nucleotide sequence ID" value="XM_002839037.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFI1"/>
    <dbReference type="FunCoup" id="D5GFI1">
        <property type="interactions" value="917"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GFI1"/>
    <dbReference type="GeneID" id="9188222"/>
    <dbReference type="KEGG" id="tml:GSTUM_00006910001"/>
    <dbReference type="eggNOG" id="KOG0478">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000995_7_2_1"/>
    <dbReference type="InParanoid" id="D5GFI1"/>
    <dbReference type="OMA" id="AFFKCNV"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031261">
        <property type="term" value="C:DNA replication preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097373">
        <property type="term" value="C:MCM core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005656">
        <property type="term" value="C:nuclear pre-replicative complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902975">
        <property type="term" value="P:mitotic DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006279">
        <property type="term" value="P:premeiotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17755">
        <property type="entry name" value="MCM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.10:FF:000003">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1640.10:FF:000011">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000217">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.10">
        <property type="entry name" value="mini-chromosome maintenance (MCM) complex, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008047">
        <property type="entry name" value="MCM_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF66">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21128">
        <property type="entry name" value="WHD_MCM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01660">
        <property type="entry name" value="MCMPROTEIN4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 15" id="KW-0235">DNA replication</keyword>
    <keyword evidence="10 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 15" id="KW-0347">Helicase</keyword>
    <keyword evidence="7 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="17">
        <location>
            <begin position="377"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00073498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU368062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="CAZ83274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="797" mass="88445" checksum="698BD5071341C6C6" modified="2010-06-15" version="1">MSSASVLRSRHGGEIEGSTPLRSRRGDLQFSDVNATPTRRRLLYVDEQGMPVRSGAGGGDVVHPLSDAATYSGGPTSDLPDGPRRLIWGTNVSVEESTQAFNKFLREFKRRYRMRMDGEFVAPGEGDELVYVEMLKQLRELGTSNMNLDVQNLKSFPPTKRFYHQLHAYPQEIIPIMDTCVKDTMLEMLEGAGASRAEYDACLERIYKARPFNLEKTVNMRDLNPADIDKVISIKGLVIRVTSIIPDMNKAFFRCHVCGHTVTVEIDRGKIAEPTVCPREVCKTPNSMQIIHNRSDFSDKQVIKLQETPDSVPDGQTPHSVSLCCYDELCDVAKAGDRVEVTGIFRSVPKVDKRRMVRKLTDEEVEKIKAIAARYDVYELLSRSLAPSIWEMEDVKKGVLLQLFGGANKTFERGGAPRYRGDINILLCGDPSTSKSQLLQYVHKIAPRGVYTSGKGSSAVGLTAYVTRDPETRQLVLESGALVLSDGGVCCIDEFDKMNEATRSVLHEVMEQQTVSIAKAGIITTLNARTSLLASANPIGSKYNPNLPVPSNIDLPPTLLSRFDLVYLVLDRVDETHDRRLARHMLGMYLEDNPENASGNLEIVPIEQLTAYISYARQNIHPKLTEEAGEELVRAYVDLRKLGEDVRAAERRITATTRQLESMIRLSEAHAKMRLSEEVTIDDVHEAVRLIRSAIKESATDPVTGRIDMDLLGGISNSERRRKGDMKNAIIGLLDGMAKGAGGARYNDVLKRLQEQSTGITVASGEFADACRTLEAEGLIRVSGEGARRAIRKISSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>D5GFT4</accession>
    <name>D5GFT4_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">GSTUM_00007066001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430220">
        <property type="protein sequence ID" value="CAZ83377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839186.1">
        <property type="nucleotide sequence ID" value="XM_002839140.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GFT4"/>
    <dbReference type="GeneID" id="9187288"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007066001"/>
    <dbReference type="HOGENOM" id="CLU_1929124_0_0_1"/>
    <dbReference type="InParanoid" id="D5GFT4"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="CAZ83377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="131" mass="14495" checksum="B62FB8D40C0D3E2D" modified="2010-06-15" version="1">MAQVLGDPVSPAGDANDCLSNVKPTTCRSERCDTLLQHLTDEPNNLTTPKPTGTLGSTHLNGQTEKQFKKIHRVILPQENSQQSLGYLTPFQKILIHPIPINLPVPCPFLATPSLTVTRATKIRCEFREPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>D5GG24</accession>
    <name>D5GG24_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">alpha-1,2-Mannosidase</fullName>
            <ecNumber evidence="13">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">GSTUM_00001932001</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N(4)-(alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 9A1,2,3B1,2,3) + 4 H2O = N(4)-(alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2) + 4 beta-D-mannose</text>
            <dbReference type="Rhea" id="RHEA:56008"/>
            <dbReference type="Rhea" id="RHEA-COMP:14356"/>
            <dbReference type="Rhea" id="RHEA-COMP:14367"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28563"/>
            <dbReference type="ChEBI" id="CHEBI:59087"/>
            <dbReference type="ChEBI" id="CHEBI:139493"/>
            <dbReference type="EC" id="3.2.1.113"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>N(4)-(alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 8A1,2,3B1,3) + 3 H2O = N(4)-(alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2) + 3 beta-D-mannose</text>
            <dbReference type="Rhea" id="RHEA:56028"/>
            <dbReference type="Rhea" id="RHEA-COMP:14358"/>
            <dbReference type="Rhea" id="RHEA-COMP:14367"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28563"/>
            <dbReference type="ChEBI" id="CHEBI:59087"/>
            <dbReference type="ChEBI" id="CHEBI:60628"/>
            <dbReference type="EC" id="3.2.1.113"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the glycosyl hydrolase 47 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="13"/>
    <dbReference type="EMBL" id="FN430232">
        <property type="protein sequence ID" value="CAZ83467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839276.1">
        <property type="nucleotide sequence ID" value="XM_002839230.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GG24"/>
    <dbReference type="FunCoup" id="D5GG24">
        <property type="interactions" value="814"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GG24"/>
    <dbReference type="GeneID" id="9181811"/>
    <dbReference type="KEGG" id="tml:GSTUM_00001932001"/>
    <dbReference type="eggNOG" id="KOG2431">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003818_3_0_1"/>
    <dbReference type="InParanoid" id="D5GG24"/>
    <dbReference type="OMA" id="AAFKHSW"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004571">
        <property type="term" value="F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001382">
        <property type="entry name" value="Glyco_hydro_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050749">
        <property type="entry name" value="Glycosyl_Hydrolase_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036026">
        <property type="entry name" value="Seven-hairpin_glycosidases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742:SF55">
        <property type="entry name" value="ENDOPLASMIC RETICULUM MANNOSYL-OLIGOSACCHARIDE 1,2-ALPHA-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE ALPHA-1,2-MANNOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01532">
        <property type="entry name" value="Glyco_hydro_47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00747">
        <property type="entry name" value="GLYHDRLASE47"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48225">
        <property type="entry name" value="Seven-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0106">Calcium</keyword>
    <keyword evidence="7 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="595"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="182"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="314"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="441"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="480"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="570"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="381"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="CAZ83467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="619" mass="70552" checksum="5D599BEA7E4BA348" modified="2010-06-15" version="1">MSFSIPKNVPSFTSPNREFEDGAVGGLFGANRDLPLYKDKPYYQRSGRDRRKMYRRKRFLGGLIAIFGLFYWFGYFPGTGTSILPIKKKTKEIVNWEDRREKVKEAFTLSWNAYEKHAWGNDQFFPVTKAGRQMIPKGMGWIIVDALDTMMIMNLTKPLEKAQHWVEHSLTFEQDSEVNTFETTIRMLGGLLSAHYLSQDLKLVPVKKAVEDMYLNKATDLADRLLGAFDSPSGVPFASVNLKSREGVPSHADGGASSTAEAATLQLEMKYLSKLIGEALYWKKAEKDGLVPIFVYADTGNFRGHEIRLGSRGDSYYEYLIKQYLQTKNQEPIYLDMYNAAMSGVKKHLIAKSHPSHLTFIAELPNGIGGELSPKMDHLVCFMPGLFALGATEGLTVEEARKAEKGVKWGVRQEEDLKLARELMRTCYEMYNVTATGLAPEIAWFNLKHGKSGDGEFLKEDPSKDIIVKPRDAHNLQRPETVESLFVMWRITGDEIYREWGWKIFEAFLEHTAVGEGAKDGFTSLDNVNEIPPKQRDNMESFWLAETLKYLYLLFSPNDILPLNEVVFNTEAHPFPKFEMGHLFETGWKRLPRDKEGVIIKEGPKEEDAENPKKDKDEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGA5</accession>
    <name>D5GGA5_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">L-lactate dehydrogenase (cytochrome)</fullName>
            <ecNumber evidence="15">1.1.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">GSTUM_00007293001</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">Mel28</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(S)-lactate + 2 Fe(III)-[cytochrome c] = 2 Fe(II)-[cytochrome c] + pyruvate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:19909"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16651"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.1.2.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:19910"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">In the C-terminal section; belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14">In the N-terminal section; belongs to the cytochrome b5 family.</text>
    </comment>
    <dbReference type="EC" id="1.1.2.3" evidence="15"/>
    <dbReference type="EMBL" id="FN430253">
        <property type="protein sequence ID" value="CAZ83548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839357.1">
        <property type="nucleotide sequence ID" value="XM_002839311.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGA5"/>
    <dbReference type="STRING" id="656061.D5GGA5"/>
    <dbReference type="GeneID" id="9183146"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007293001"/>
    <dbReference type="eggNOG" id="KOG0537">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0538">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020639_1_0_1"/>
    <dbReference type="InParanoid" id="D5GGA5"/>
    <dbReference type="OMA" id="WDITEFI"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004460">
        <property type="term" value="F:L-lactate dehydrogenase (cytochrome) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02922">
        <property type="entry name" value="FCB2_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000062">
        <property type="entry name" value="Cytochrome b2, mitochondrial, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000262">
        <property type="entry name" value="FMN-dep_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037396">
        <property type="entry name" value="FMN_HAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037458">
        <property type="entry name" value="L-MDH/L-LDH_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578:SF104">
        <property type="entry name" value="CYTOCHROME B2, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578">
        <property type="entry name" value="S -2-HYDROXY-ACID OXIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01070">
        <property type="entry name" value="FMN_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00363">
        <property type="entry name" value="CYTOCHROMEB5"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51349">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0288">FMN</keyword>
    <keyword evidence="5" id="KW-0349">Heme</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="17">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="FMN hydroxy acid dehydrogenase" evidence="18">
        <location>
            <begin position="97"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00052399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00061137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00061589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00066458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00068515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="CAZ83548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="481" mass="52232" checksum="02D12EFEC65A1229" modified="2010-06-15" version="1">MYTLKDVAVHENPGDCWILIKGRVYDVTEFLPQHPGGAEIILSYAGADATGAYESVHTSSLLETPPPGFRLLGPLSPSELPPVSRNPGLDVELHAKPPLDTIINTFDFEAVAERTVTPKTWAFYSSAATDLLSMKLNRRAFDRVLFRPRLMRNVKSVDTRTKILGFSTGVPFFIAPTAMQGMINPDGEKAVAMGAGEEKVIHIISTNSSHPISDIVSSGKGPEQQTHFLQLYVNTDRQKTAQLLANAKSCGLKAVFVTVDAHISGKREADERLKVDVPVRSAVSGAISHNDKKGGGMGRLMGLYIDRTLNWEDIPWIKSVAGGLPIVLKGIQTAADARLAAEYGVQGIVLSNHGGRNLDTSPPALYTLLEIHKVCPEIFNSLEVYIDGGIRRGTDIFKALCLGATAVGVGRPYLYALNYGAEGVAHLTQILKDELETTMRMCGVTDLSGVHPGLVNTREIDALVEDGIEHSYVKWRPKSKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGC8</accession>
    <name>D5GGC8_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="4">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">GSTUM_00007317001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430264">
        <property type="protein sequence ID" value="CAZ83571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839380.1">
        <property type="nucleotide sequence ID" value="XM_002839334.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGC8"/>
    <dbReference type="STRING" id="656061.D5GGC8"/>
    <dbReference type="GeneID" id="9183169"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007317001"/>
    <dbReference type="eggNOG" id="KOG0504">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_610018_0_0_1"/>
    <dbReference type="InParanoid" id="D5GGC8"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF165">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="25"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="48"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="73"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="CAZ83571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="449" mass="49726" checksum="EDE53988380F0820" modified="2010-06-15" version="1">MLPYNPYTNSKGLFNPRVMEVRPQAPSTPVQQVSAPQGGQPQVKHPQGSAQQAGVTQSNHPRRSKPTHICPEPPRKLSGEKHHVEMTSGDISMPCLPARKRSRIEELDTANTEASATEIILVSKRRISIQNVPSWVLMKVLEWQVKGESTAVTVREIANLVLVSKEFRILFHPELVKEGIRHAARTKNIVLLQHLCPKAQTLNFPAWTSGKGCKSQGARNASLSPVGAPLHESVGANDVTCVAFLLKAGADPNFLDCRHETALHRAAKSAGPLILVLLVKAGGNPEIRNLQLWTPFDIAIASRNFAIMYSLWRLGHQVDGRRDPTGSSPLHKAIDTDCLPTLQLVLAFEPKLNVKDWSGATPLIYAICQGKSSKVIDAIMRLNPNALAKDRAGKTALYYMYVRNDAVIKGLFARYYCGSEFNKGLKDAQKKSYRRYETTPGEFSFIYHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGF1</accession>
    <name>D5GGF1_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Vacuolar-sorting protein SNF7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Vacuolar protein-sorting-associated protein 32</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">GSTUM_00007350001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF7 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430264">
        <property type="protein sequence ID" value="CAZ83594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839403.1">
        <property type="nucleotide sequence ID" value="XM_002839357.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGF1"/>
    <dbReference type="FunCoup" id="D5GGF1">
        <property type="interactions" value="617"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GGF1"/>
    <dbReference type="GeneID" id="9183192"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007350001"/>
    <dbReference type="eggNOG" id="KOG1656">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071097_1_0_1"/>
    <dbReference type="InParanoid" id="D5GGF1"/>
    <dbReference type="OMA" id="MKQIHGG"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000815">
        <property type="term" value="C:ESCRT III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904669">
        <property type="term" value="P:ATP export"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070676">
        <property type="term" value="P:intralumenal vesicle formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1060">
        <property type="entry name" value="ESAT-6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761:SF10">
        <property type="entry name" value="GH13992P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0967">Endosome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="13"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00042586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="CAZ83594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="208" mass="23441" checksum="077B4C01B657469C" modified="2010-06-15" version="1">MWSWFGGGAAKKKDSAKDAILKLREQLEMLQKKEKHLEGEIAEQDAIARKMVQTNKNAAMTALRRKKTHETHLASTVAQINTIEQQMHSIETANLNFETLKVMQSASDAMKKIHNGMNLDKVDMTMEEIREQQQIAEEIGNAIVNMSEPTDESELLKELDNIKQEQLDTQMLSAPSAPITATPQTTKGKMIEEDEDAELMKLKAEMAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>D5GGR2</accession>
    <name>D5GGR2_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat and J domain-containing co-chaperone DNJ1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00007480001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="FN430275">
        <property type="protein sequence ID" value="CAZ83684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839493.1">
        <property type="nucleotide sequence ID" value="XM_002839447.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GGR2"/>
    <dbReference type="STRING" id="656061.D5GGR2"/>
    <dbReference type="GeneID" id="9185606"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007480001"/>
    <dbReference type="eggNOG" id="KOG0624">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015935_0_1_1"/>
    <dbReference type="InParanoid" id="D5GGR2"/>
    <dbReference type="OMA" id="PFAHFQH"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051787">
        <property type="term" value="F:misfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000224">
        <property type="entry name" value="DnaJ and TPR domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051727">
        <property type="entry name" value="DnaJ_C3_Co-chaperones"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44140">
        <property type="entry name" value="LD25575P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44140:SF2">
        <property type="entry name" value="LD25575P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 9" id="KW-0732">Signal</keyword>
    <keyword evidence="4 7" id="KW-0802">TPR repeat</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003072777" description="Tetratricopeptide repeat and J domain-containing co-chaperone DNJ1" evidence="9">
        <location>
            <begin position="23"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="30"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="64"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="10">
        <location>
            <begin position="401"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="466"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00073740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="CAZ83684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="521" mass="57720" checksum="23361BEBC51D3EF1" modified="2010-06-15" version="1">MIVRLGTLALAAALFSVHFVACDTSFNQPVQSLIESANALLAKGDMHGALDHFEAAIKKDPTNYLTIFKRGATYLSLGRSNQASADFDAVLSLKPDFEAALLQRAKLKSRTGDWNAARRDYKKAGGVTGKERIAELEEAERAVKTAMEAEKKGDYEACVTHAGTAIMVASGLHSLRSLRARCRLKRGEVHEAVGDLTHLVQLLPGNIDPHLQIANLLYFSVNDYDRAVAQLRKCLHSDPDSKPCSKRFRRIKDLEKSVAKVRTLVEKRQYTSATRLLVGHSGETGLIDEVKEEVADIRKAGYYTSACPEDLLLWLLETTCEVYTETKNNKKATPHCAAALSLNPNSLPALLSKATTQLAEDLFEEAIRTLEHAKQTHPDSQPLLQKLQEAHTLLRRSKAKDYYKVLSVPRDASDREIKRAYRALTKKYHPDKYRGELSQEEILKNMAAINEAYEVLSNEELRARFDAGDDPNEQDGPGRAWGHGGQNFMFRGGPGGGGGQQFMFRGDGGQGDPFGGFKFQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>D5GH02</accession>
    <name>D5GH02_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">GSTUM_00007639001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430297">
        <property type="protein sequence ID" value="CAZ83795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GH02"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007639001"/>
    <dbReference type="HOGENOM" id="CLU_3425155_0_0_1"/>
    <dbReference type="InParanoid" id="D5GH02"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="CAZ83795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="22" mass="2455" checksum="34E2DD37F81D13CF" modified="2010-06-15" version="1">MYHSIASHDLTISLHAITNAMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>D5GH05</accession>
    <name>D5GH05_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="11">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">GSTUM_00007642001</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">Mel28</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SF3B1 family.</text>
    </comment>
    <dbReference type="EMBL" id="FN430297">
        <property type="protein sequence ID" value="CAZ83798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839607.1">
        <property type="nucleotide sequence ID" value="XM_002839561.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="D5GH05"/>
    <dbReference type="FunCoup" id="D5GH05">
        <property type="interactions" value="980"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GH05"/>
    <dbReference type="GeneID" id="9185052"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007642001"/>
    <dbReference type="eggNOG" id="KOG0213">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002242_0_1_1"/>
    <dbReference type="InParanoid" id="D5GH05"/>
    <dbReference type="OMA" id="LVMNYVW"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="C:U2-type prespliceosome"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140727">
        <property type="term" value="P:siRNA-mediated pericentric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="entry name" value="Splicing factor 3B subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000069">
        <property type="entry name" value="Splicing factor 3B subunit 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Splicing factor 3B subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054573">
        <property type="entry name" value="PP2A/SF3B1-like_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015016">
        <property type="entry name" value="SF3b_su1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038737">
        <property type="entry name" value="SF3b_su1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12097">
        <property type="entry name" value="SPLICING FACTOR 3B, SUBUNIT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22646">
        <property type="entry name" value="PPP2R1A-like_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08920">
        <property type="entry name" value="SF3b1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Splicing factor 3B subunit 1" evidence="9">
        <location>
            <begin position="236"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatase PP2A regulatory subunit A/Splicing factor 3B subunit 1-like HEAT repeat" evidence="10">
        <location>
            <begin position="1002"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="13"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="58"/>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="8">
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            <begin position="113"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
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            <begin position="193"/>
            <end position="256"/>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
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            <begin position="68"/>
            <end position="78"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
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            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005754"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
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            <dbReference type="ARBA" id="ARBA00022737"/>
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            <dbReference type="ARBA" id="ARBA00023187"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="Pfam" id="PF22646"/>
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        <source>
            <dbReference type="EMBL" id="CAZ83798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="1217" mass="135973" checksum="361A372C8C892A8C" modified="2010-06-15" version="1">MADAEFEEIKRMQQERNAMKSKSKAFDPSTQRKDNSTKVALTDTFDTTLYDRANGDKFAGYNTSIPANDDDDDVDMDGGDGTRRLVGQYTASKEIISEFANAGDDDMDLMSAKAKGGQIADRETDYQKRRFQRQLTPTRKDAFASDGVEGEGRSYREVMLEKELEREEERVRRQIEEKLKNGDGKMDGVEHEATLKDKEADVENRDGATDGTRKRRRRWDVGVDDNAVPVKEEDASKKRSSRWDVAAPPDATPVVKRSRWDQAPSLTAATPVGNQGLVTPIHPTVNATPVASLPITFGHGDRNAPLSDEELDEMLPKEGYKILEPPPGYAPIRTPARKLVSTPAPMASSSGIGGFMMQEPENARILGKQMPTDIPGVGDLQFFKAEDMVYFGKLVDGGDENSMTVEELKERKIMRLLLKVKNGTPPMRKTALRQLTDNARQFGAGPLFNQILPLLMEKTLEDQERHLLVKVIDRILYKLDDLVRPYVHKILVVIEPLLIDQDYYARVEGREIISNLSKAAGLAHMISTMRPDIDHVDEYVRNTTARAFAVVASALGIPALLPFLRAVCRSKKSWQARHTGVKIVQQIPILMGCAILPHLKGLVDCIGPNLEDEQAKVRTVTSLAIAALAEAASPYGIESFDTILNPLWTGAKKQRGKGLAGFLKAVGYIIPLMDEEYANYYTSEVMEILLREFQSPDEEMKKVVLKVISQCAGTEGVTAAYLKENVLPEFFKAFWVRRMALDKRNYRQVVETTVDLGQKVGCGEIIERIVDHLKDESEPYRKMTLESVEKVIASLGAADIGERLEERLVDGVLFAFQEQTMEDVVMLNGFGTVVNALGTRCKPYLPQIVSTILWRLNNKSSAVRQQSADLISRIAVVMKQCGEDALMGKLGVVLYEYLGEEYPEVLGSILGALRSIVTVVGINSMQPPIKDLLPRLTPILRNRHEKVQENTIDLVGRIADRGAEFVSAREWMRICFELLDMLKAHKKGIRRAANNTFGFIAKAIGPQDVLATLLNNLRVQERQSRVCTAVAIGIVAETCAPFTVLPALMNEYRVPELNVQNGVLKSLSFLFEYIGEMAKDYVYAVTPLLEDALIDRDQVHRQTAASVVKHVALGVVGLGCEDAMMHLLNLLYPNMFETSPHVIDRIIEAIDAIRMAIGPGLVMNYVWAGLFHPARKVRTPYWRLYNNAYVQSADCLVPFYPNLDEEKLQRNDLMIVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHB1</accession>
    <name>D5GHB1_TUBMM</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(Perigord truffle) hypothetical protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">GSTUM_00007730001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">Mel28</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="FN430308">
        <property type="protein sequence ID" value="CAZ83863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_002839672.1">
        <property type="nucleotide sequence ID" value="XM_002839626.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="D5GHB1">
        <property type="interactions" value="456"/>
    </dbReference>
    <dbReference type="STRING" id="656061.D5GHB1"/>
    <dbReference type="GeneID" id="9182568"/>
    <dbReference type="KEGG" id="tml:GSTUM_00007730001"/>
    <dbReference type="eggNOG" id="KOG1910">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000592_0_0_1"/>
    <dbReference type="InParanoid" id="D5GHB1"/>
    <dbReference type="OMA" id="IQLKWNN"/>
    <dbReference type="Proteomes" id="UP000006911">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019441">
        <property type="entry name" value="FMP27/BLTP2/Hobbit_GFWDK_RBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019415">
        <property type="entry name" value="FMP27_SW_RBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019449">
        <property type="entry name" value="FMP27_WPPW_RBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045167">
        <property type="entry name" value="Hobbit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15678">
        <property type="entry name" value="ANTIGEN MLAA-22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15678:SF6">
        <property type="entry name" value="BRIDGE-LIKE LIPID TRANSFER PROTEIN FAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10344">
        <property type="entry name" value="Hobbit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01214">
        <property type="entry name" value="Fmp27_GFWDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01215">
        <property type="entry name" value="Fmp27_SW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01216">
        <property type="entry name" value="Fmp27_WPPW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="FMP27 SW motif-containing RBG unit" evidence="4">
        <location>
            <begin position="1088"/>
            <end position="1190"/>
        </location>
    </feature>
    <feature type="domain" description="FMP27/BLTP2/Hobbit GFWDK motif-containing RBG unit" evidence="3">
        <location>
            <begin position="1208"/>
            <end position="1366"/>
        </location>
    </feature>
    <feature type="domain" description="FMP27 WPPW motif-containing RBG unit" evidence="5">
        <location>
            <begin position="1621"/>
            <end position="2133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="649"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1004"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1854"/>
            <end position="1881"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1949"/>
            <end position="1976"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2475"/>
            <end position="2580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2587"/>
            <end position="2606"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2721"/>
            <end position="2789"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1004"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1049"/>
            <end position="1058"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="2488"/>
            <end position="2497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2524"/>
            <end position="2535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2549"/>
            <end position="2564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2761"/>
            <end position="2776"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="CAZ83863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000006911"/>
        </source>
    </evidence>
    <sequence length="2841" mass="320689" checksum="14D4558B90AFE595" modified="2010-06-15" version="1">MATAYHLPTFLLGVVVLAYLSTFVLFAVLRILTGISIQRIGYFSFKRMSYEPKNGIKLEVRKLGLLLHRPTFAQPTWISIAISDSQFSVDLRKTQEEEKGRGMRRNTRAWEGISKLRESLKKVHKLVKWLQMVDIVFTNTNLSIADVGSVQVGSMTMILDTRRKTAERNRMFDHTTDLKEDQNPIEWMFTARSVLFFPEKKREPIEILDHCNFNTYGLLENGLDVFRDAAMALKVGKITIPLDEILELRLGAIMEEMSLPGSATERMAETVMESKEFLESLLRGLKEVQFAIGYLLITKSVRSIQPSGKPLKVLVGMKELAMDVHRLDQKSPAHRMYFSPQDIAHQALLAAISISVSVDDGVRKDKFFYIPMVTMTSKTTLPSKTIQLVEKSESNRNTNILFANVVVTSPSIDLEPRHLPILLALVQSKPEKPPSGSRERTHLISKLLPKASINFSVHEPVIRILLPSNDPERRAAGDMDMLISSLSSISAVADSSHESEGQNDYSLSCSFRITSHHLYYQAHSGEKHDLLQTDAFDIKFQVNASPEVEVTAHAYLETFSLRLVRPEIVQGIKQTVGQFHRNVKSDKLSKPETSDDPNLLRRIPLWIDQFKMEGIDFSVEVAGVDEEISEFTRGVALQLDSWTIEYRARKSESRTKPPPRRRAASRTLSIDDTRGSKLPPAGPKNPTDCRKLSLHIRGLEGYMVDSPDSWEQEPFLNIPHFDIAFATSGDLEGPVLHISSHSKLLSLNYSLYRHYTIIVAAKVLIEAFGGTSTGGEAGYSQIKPVDTLRPGAVGDMDLVESPISDFREYVSVDFKVQHVRIKSSMPDDPPMMLEVFGMDTGRQRWGFPFLKAKSVRLYAESPKVMNCWARMISIRQFRADLKEGGHGYGDQLQEEKSIDLFADAVRLAIPHQLVLYKVTDNVINTAKACQQMHHRFKTGTNEYILEKKAESAKKLPRLSLNTKALLLELEDDPFETQLGLIYRLGLSEQKKRLAREAAFDAKVKKMGENERRKSSETTRTGLKPENPLQTSTSRFRGRTKTWGGSSHHVHAESPETRSKSAHRGPKNMRYDPESAAGPSDDASVSVEEAWERLQEHNSAAWIKRMRWAHGYHSTRMKESREQFWGHDDVPYDGEDTENILGLPMRPALMAAYFNDVEVVVDKPSFPLEQLPKFMHRVGKGLPEKTEFALLVPVSVKVDFSEGRILLRDYPLPLIHVPRMRPSQANRSPAWSLKADFVLAEELRGPESMRHVRVNIVPPTSGPGPSKGGFAVDVRRTVSPVKSYSEIAVAISTSFATRITWCTSYQPAIQDMMMVFETFTKPHFDPSDRTGFWDKIRLVLHSQISVKWKGDGDVHLTLKGSRDPYQITGIGAGFVLCWRGDVNWEIGRDEDPRKFMVVDSEEFLLAIPDFARNAKDEFDSATPAENKSVLSFSSCESAALFKKVIMKLSGRVRWLVGLMFEKEISEGQLGVCEGKTRSFEFKSHYEVTLKNPTYAKAPPGQVYDAFRGFRSHHLHLSLAILSPIDRDWSPANTKPSTSYNTIHLTPRFFTHFFSWWGLFSGVMSLPIRQGPLWPGPEKSNKKFGRHLATIKYKLCISPLFISHMYKHNDKGEAGKDVVVATGLKAKLDSLMVDIHQRREETLPNQQSTAMRINQAELDFHSADIRAVSALIAGATPDELMKQAAEGPPLGLTETIRYSGDMSQFTIPDGDYSWIDMDDFIELDWVLPNNKTPKTKIMPLAFTPRFTYFRQTDHTDLKGCVLNPCSPFGDEPTHDCLMSENNDPREIQCGLVQARLEKVNEQMNRNKEALDNLAQYTKFNPEDLELKAQSSELIEQSSILFNKLDFLQTMLRRMSSKLDGGDKPGVSKNPSHERHGSDADSELIGVDSSPFDYVSDFDNRFIVHNMQAKWNNSLRNIILKYIHQVGQRRGFVYYMSRRAVKFILDIVQEQKQSGRDNTSATGEYATSNPTSTLNSDTDSMTLESRIQQLLDDDNKYVVADEKPNDPEENGAPARTYARDDLTGDVADDYLPLNSYHIRLIAPQIQMQSEKNKGAAVLVVAQGMQLKVVSIMDRDRVGDEVSGLVQRRFALNLENTQFFVSHRQDFAPQSMSLHSANRYGAPGGSSWPPWVPLESMFDFQNFPVGFSRVVERTSATLRYDKYNSLRLKYSDQVSGEEKTFSAKGGPLDVERRIDHVWVDFPRVEASCDSSQYFAMYIIVLDLLLYAEPLEKLRNEKLEKIMLASDFSDLTGAPEMVERLQSRIHQLEEIKTYFQINSQNLDIEGWRGRVAVDEDLASCEEELFFMMKAITTAQRKHEDRTSQASGILRWYLTASEIVWHMLRDKADPLMDIRLKNAGYKRMDNADGSNFNTVEVEMMEGFNLLPDAVYPEMIAPFFDQSRTVVDVRQTKILRVYWHMLEAIAGIPVMDHFEVNLFPLKIQLEREIGDRIFEYIFPGVGTQAFENGGFSPFLVHSIKPIPDGSDTDESDEDHDSHHMDDSSSTKSDVSPVEIKLRPTLSLTADHRPGSSSSASKPRSNSFKASNAGFRGTFMPSLTPTHKSSAESLSVKSRKSTEGPAPSIMGLEKNKKLQIFNRSSNHSGRNEKRSDELSQMMSRASNFMTLAYIKIPSVVLCLSYKGRGDNNFEDVHEFVFRMPMLEYRNKTWSNLDLAIRLKKDIIRALISHTGAILQNKLTHHRPNKTQPNLLRMMANNGSLATNPSFVTTDSDHPGFDTPSEYASSTHASMPISRPSTGLHLKPPSVMRPMTSPSSASSSTSMSFPPSPLDRAEEEAGERGHGIFHNTLGRHLSNLGVVARAQNGIADESEESIKKKSRLLLGKKVFGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-06-15" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>D5GHJ9</accession>
    <name>D5GHJ9_TUBMM</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">superoxide dismutase</fullName>
            <ecNumber evidence="4">1.15.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">GSTUM_00007953001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Tuber melanosporum (strain Mel28)</name>
        <name type="common">Perigord black truffle</name>
        <dbReference type="NCBI Taxonomy" id="656061"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Pezizomycotina</taxon>
            <taxon>Pezizomycetes</taxon>
            <taxon>Pezizales</taxon>
            <taxon>Tuberaceae</taxon>
            <taxon>Tuber</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2010" name="Nature" volume="464" first="1033" last="1038">
            <title>Perigord black truffle genome uncovers evolutionary origins and mechanisms of symbiosis.</title>
            <authorList>
                <person name="Martin F."/>
                <person name="Kohler A."/>
                <person name="Murat C."/>
                <person name="Balestrini R."/>
                <person name="Coutinho P.M."/>
                <person name="Jaillon O."/>
                <person name="Montanini B."/>
                <person name="Morin E."/>
                <person name="Noel B."/>
                <person name="Percudani R."/>
                <person name="Porcel B."/>
                <person name="Rubini A."/>
                <person name="Amicucci A."/>
                <person name="Amselem J."/>
                <person name="Anthouard V."/>
                <person name="Arcioni S."/>
                <person name="Artiguenave F."/>
                <person name="Aury J.M."/>
                <person name="Ballario P."/>
                <person name="Bolchi A."/>
                <person name="Brenna A."/>
                <person name="Brun A."/>
                <person name="Buee M."/>
                <person name="Cantarel B."/>
                <person name="Chevalier G."/>
                <person name="Couloux A."/>
                <person name="Da Silva C."/>
                <person name="Denoeud F."/>
                <person name="Duplessis S."/>
                <person name="Ghignone S."/>
                <person name="Hilselberger B."/>
                <person name="Iotti M."/>
                <person name="Marcais B."/>
                <person name="Mello A."/>
                <person name="Miranda M."/>
                <person name="Pacioni G."/>
                <person name="Quesneville H."/>
                <person name="Riccioni C."/>
                <person name="Ruotolo R."/>
                <person name="Splivallo R."/>
                <person name="Stocchi V."/>
                <person name="Tisserant E."/>
                <person name="Viscomi A.R."/>
                <person name="Zambonelli A."/>
                <person name="Zampieri E."/>
                <person name="Henrissat B."/>
                <person name="Lebrun M.H."/>
                <person name="Paolocci F."/>
                <person name="Bonfante P."/>
                <person name="Ottonello S."/>
                <person name="Wincker P."/>
            </authorList>
            <dbReference type="PubMed" id="20348908"/>
            <dbReference type="DOI" id="10.1038/nature08867"
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